LOCUS       DAOTQD010000001        25673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_2000_length_25673_cov_8.088610, whole genome shotgun sequence.
ACCESSION   DAOTQD010000001 DAOTQD010000000
VERSION     DAOTQD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 25673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25673
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_25673_cov_8.088610"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..1549
                     /locus_tag="P5570_00005"
     CDS             323..1549
                     /locus_tag="P5570_00005"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRR39144.1"
                     /translation="MNFSFDVKKIRDDFPALNQKVYDKPLVYFDNAATTQKPKVVIDI
                     INKSYSEYNSNVHRGVHYLSEKMTERYEAAREKVRNFLNAKYAHEIIFTKGTTDSINA
                     VAFSFGEAYVKEGDEIIVSEMEHHSNIVPWQMLCQRKGAYLKVIPFNDDGELLLDEYK
                     KLINSKTKLVAVVHASNSLGTVNPVKEIISIAHKNNVPVLIDGTQAIQHRKIDVQDMD
                     CDFYCFSGHKIYGPTGIGILYGKEHWLKEMPPYQGGGDMIDRVTFEKTTYAQLPAKFE
                     AGTPDYIGAVGLGTALDYLNSIGLDNLYKYEEQLLKYGTNKLKEIDGLKIYGMAKDKT
                     CVFSFLLNNIPAYDTGMILDKMGIAVRTGHHCTQPIWDHYRVEGSARASLAFYNTFEE
                     IDYFCETLKQVQKMFE"
     gene            1772..2749
                     /locus_tag="P5570_00010"
     CDS             1772..2749
                     /locus_tag="P5570_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054752.1"
                     /note="catalyzes the interconversion of alanine and
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRR39145.1"
                     /translation="MLILTQKEIKKILPLKKIDIVINAVQKAFSDYGQNKVQMPAKLY
                     LYFKEFNGDLRIMPSFSETLKLAGTKIVNVHPDNYKKRLKSIMAVIVLNDARTGLPLT
                     LMDGTYITALRTGAASAVATKYLARKEAKSLGVIGAGSQAITQILAIAQVRKLSEILV
                     YDINEEAIKNLEKILANEKIKIKKASLKETAQKDILVTITPAKKPIIKKEWILPGTHI
                     NAIGADAVGKEELDPQILKKAKIIVDDWAQASHSGEINVPLKKGILKKKDVYAYLSEI
                     ICGKKIGRKNNKEITIFDSTGLAVQDLYTANIVYNLAKKKKLGKQIKII"
     gene            2754..3656
                     /gene="cysK"
                     /locus_tag="P5570_00015"
     CDS             2754..3656
                     /gene="cysK"
                     /locus_tag="P5570_00015"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRR39146.1"
                     /translation="MTKKQNNLYSVLELIGNTPLIKIGDIFAKLETVNPSGSVKDRMA
                     WYMIKKAEERKELKPGNKIIEVTSGNTGISFAMISAVRGYKFTAIMPESMSVERRKMI
                     KAFGAEIFLTPAKKDMAGAVEQYQKLIKANPDAWLPRQFENPDNIAAHREGLGKEIIK
                     QTKGKIDSFVAGVGTGGTLIGTAQALKKINPKIKIIAVEPAESAILSGKKPGLHKIQG
                     IGEGFIPKLVQENIAIIDEVLMIGSNEAIDTSKELAEKFGILVGISSGANVLAARRIK
                     EKYGFKNIVTVLPDRGERYLSEIF"
     gene            3665..4087
                     /locus_tag="P5570_00020"
     CDS             3665..4087
                     /locus_tag="P5570_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRR39147.1"
                     /translation="MTIEKIQNEIIKEFENLDDDLKKYNYLIKLGRELKPVDSKYKTD
                     NYLIRGCQVNTWYHSECKDGKMFYNIDSVSLITRGLISLLLRICSAQTPEDIKNTEFY
                     FIDKIGLRNQFSPLKDNSLFKLVNKIKADADFWSNNTK"
     gene            4099..4572
                     /locus_tag="P5570_00025"
     CDS             4099..4572
                     /locus_tag="P5570_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HRR39148.1"
                     /translation="MDDINQTKCPIPNSKHFLVYESSLKFCRTFYAVLVLISFLFRNQ
                     WLLLATIVLMFLRLFSVKLDLPYQFHALFLRKLLKDKSKPVMKEKSELNFVFGMMAIL
                     LLIGFLLIYFNKFVDFAWIFILMIDLLMFLAALVGFCVATLMYVFFKKIFKKQKK"
     gene            4597..5733
                     /locus_tag="P5570_00030"
     CDS             4597..5733
                     /locus_tag="P5570_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR39149.1"
                     /translation="MEKSEPSQKTKIELGEPNYLNKNCLLANSLGHSPYKRCQYCESN
                     FSNCIFLQYQIISLGLIVFSFVLSFIMEGRISETIIFSVFALVIVYGYFFSKSTDKII
                     ETNFKRKKAEELALREKISKEEAEKLAVQERALRKRTEKLVNEYKNLDEAKTMFLNIA
                     SHQLRTPLSAMKGYLSMILDGNYPREKEKEFIKTIYRSNEREIELVNDLLDLNKLQTG
                     KLQFNFRDDVQLEDIIEEIINEFQAEAQHKGLYLVFNKPKQPLPKITADPQKLRQVIL
                     NLIDNALKYTSKGGVTVNLKPCPSSIQISVQDTGMGMMSQEKEKLFQLFSRSEEATSV
                     YPSGIGIGLYLSSEIVKAHKGKIWAESPGKGKGSTFYVELPVKK"
     gene            5935..6315
                     /locus_tag="P5570_00035"
     CDS             5935..6315
                     /locus_tag="P5570_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR39150.1"
                     /translation="MSKKVLIIEDEEVLVDMYKVELERLGFNVKYALDGEDGLEAAKE
                     ERPDLIILDLLLPGMSGMKVLRTLKDDPETKDIPVFIFTNYEAPEEREKGQRLGAEKY
                     VLKTSITPREVGELIKERLEKNNY"
     gene            6515..8078
                     /locus_tag="P5570_00040"
     rRNA            6515..8078
                     /locus_tag="P5570_00040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            8413..8880
                     /locus_tag="P5570_00045"
     CDS             8413..8880
                     /locus_tag="P5570_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HRR39151.1"
                     /translation="MLSADYIVGLTDGEGSFTVFIHPFRKGLSKYPRIECRYYIKLRE
                     DELSLLEEVKNFFGCGSIYLQKEKRLNHRNCYRFEISSYQKISEVVIPFFQKHHPRSI
                     GRKNDFDLFCKIFEIVSDKKGKHITEKELKQIKELKLQMHKYRAPLRAGNPLA"
     gene            9444..9614
                     /locus_tag="P5570_00050"
     CDS             9444..9614
                     /locus_tag="P5570_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39152.1"
                     /translation="MAFDKERNFERKMHQGDWACSECGAKITELPFEPAPDRPLYCRD
                     CWKKKRDERFQR"
     gene            9731..11185
                     /locus_tag="P5570_00055"
     CDS             9731..11185
                     /locus_tag="P5570_00055"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRR39153.1"
                     /translation="MSKFYITTTLPYINSDPHLGFALEIVQADVIARYYRQKGYEVFF
                     NTGTDEHGLKIFRKANDLGLSPKEYCDIYSEKFRKLKDALCLSYNSFIRTTDSKHIKA
                     AQEFWKRCAKNNDIYKGKYKIKYCVGCEMEKTDSDLVNGRCFLHPNQDLEVFEEENYF
                     FRYSKYQKKLLNLYQKNPQFVLPKERMNEIINFTRKGLKDFSISRVKEKMSWGVPVPG
                     DEEQVMFVWFDALVNYISCLGWPDDLKTFNNFWPGLQVAGKDNLRQQTSMWQAMLMSA
                     GLSPSRQVFIHGFITSNGQKMSKSLGNVINPFDLVNKYSTDAVRYYLLREIPPSEDGD
                     FTYEKFEKRYNSDLAAGLGNLTARVMKIAEISHFRFSLIRPAEEFKIISKQFQNKNFQ
                     KTISDTSKKYQLSLENFKFNEALIAVWDLIGFCDKYIEKERPWEQKDQKVISDLLFAI
                     EQISYLLQPILPQTADKILKQLKTHKSEILFPKI"
     gene            11182..12438
                     /gene="nadE"
                     /locus_tag="P5570_00060"
     CDS             11182..12438
                     /gene="nadE"
                     /locus_tag="P5570_00060"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRR39154.1"
                     /translation="MKKKIFIGRFQPFHKGHLEAVKWIAKNNGEIVIVIGSAQEFATK
                     ENPFSFNERKTMVQNALLKARIKKFSIFGVPDFVEDDTLWVKKVLEITKSKPEEIEVF
                     TRNAWTEYCFKKLGAKVTLHPVFLNGLSGTMIRKKIAANQKWQNLVPEPVLKYLKKNK
                     GKERIKSYSVLPEKKIVSFIKDEIKNAGARGAIMGISGGIDSSVTACLAKKALREKIV
                     FVQMPFFKTCPLINNADLLKKHLSINTKRVYLGDVFESFLGVFSKTSEIAKENLKSRL
                     KMTTLYYFAEESQLLVIGAINKSELESGFFTKYGDGGADIFPLGDLYKTEVIEMAKRL
                     KLPQQIIETIPIIDFARDRGKKQSLIIPYQRLDTILKFLDQGNFSVKEISFLTDVSFE
                     IVKEIIKRKKNSPARTNPPIICKIKN"
     gene            12512..13489
                     /locus_tag="P5570_00065"
     CDS             12512..13489
                     /locus_tag="P5570_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39155.1"
                     /translation="MNKKNLIILIISISLAMTGILKLINKSDNERKETNREVNQDEKL
                     PYLPSEKESSTSSSEETKELMAVTKTIDSDAINLTKISTQNCYAFAPAGWANIESMQS
                     VGFGYDAFSPDETSMASYGMVGLTGDFATFYGLNSAEKFIEVDLTNLGYTNFSWLSEA
                     IDVGNDYKTRTFKAIEPQKNRPVTGIFLYRTWDLSDGGFIIVLRSAITTSDKWKENSA
                     LAFNVSLSMRCTAKLQPSSSPSASSNDEVSDMIIDSYEKRQSTLDEASSEWSEGMLGY
                     ENVYSPSTGDHYWAPLDSKWETGPSGSGYYRVVNPTTGDVEKLENGFGY"
     assembly_gap    13537..13636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13688..14107
                     /locus_tag="P5570_00070"
     CDS             13688..14107
                     /locus_tag="P5570_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRR39156.1"
                     /translation="MNKKFEKLLDELRKLDLPDGKYAILGSGPLAVRGIREARDLDII
                     VTDDLYKKLQKEYSQNDKIKLGKIEIFSANGWRSKFGDLTKMIKQAEKIQGLRFVCLE
                     DLIEWKTKLGRPKDLEDIKLIKDYLKSFNNVFGDNQS"
     gene            14190..14846
                     /locus_tag="P5570_00075"
     CDS             14190..14846
                     /locus_tag="P5570_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRR39157.1"
                     /translation="MALKIKIKDPVSGLSHLIGAVLSVFGTVILISSAVQEQSSIKII
                     SFSVFGLSMIFLYASSTIYHLFGHSPEEIDIFRKIDHAMIYILIAGTYTPICLIPLWG
                     PWGWYSLILIWALAIFGISMVFFKKFWTNVPRWVATVIYVVMGWLSVVLLYPLSQKVS
                     VSFIGWLLLGGFFYTLGAYIYAQKKPNFCKQFGFHEIFHILTILGTFCHFWGIYNFII
                     "
     gene            15085..15957
                     /locus_tag="P5570_00080"
     CDS             15085..15957
                     /locus_tag="P5570_00080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRR39158.1"
                     /translation="MLIDTHSHLNFSDFEEDRNEVIKRCFENKVSMINIGTNFETSKK
                     AIEIAQQYQGKAWAAIGLHPINLDTGLIKIKNQKSKCKNWEDLTEDKFNYQKYKELSK
                     NQRVVAIGEVGLDYYWKPKTTKKKEIFKKKQKDLLFQELKLASELNLPVIFHCRMAHQ
                     DLIEILLSQKYLSFKKRRKGVMHGFVGKKEELEAYLKMGFYIGFNGIIFKNIEGINFI
                     ENIKITPLDKILIESDCPFLMPPVFTKSAIKPGRNEPASIRFIAENIADIKRVSVEKV
                     INITTQNAKQFFNI"
     gene            16004..16579
                     /locus_tag="P5570_00085"
     CDS             16004..16579
                     /locus_tag="P5570_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRR39159.1"
                     /translation="MEQVVAKEAIKINIDKPKIKKRVVMVIAFQGFRDEEYFVPKEIL
                     ESAGVEVKTASTKLGIALGDDGGEARVDFLISEVKPENFNAVIFVGGPGCLQNLDNDD
                     SYRLARETVFQKKILAAICIAPVILARSGVLKEKKATVWTSPLDTTPIKILKDNGAEY
                     IDKNVVRDGKIITANGPSAAEEFSQKILENL"
     gene            16905..17426
                     /gene="rplJ"
                     /locus_tag="P5570_00090"
     CDS             16905..17426
                     /gene="rplJ"
                     /locus_tag="P5570_00090"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRR39160.1"
                     /translation="MLKKAQKRELVKEIEKKLRENKLAVFCNFEGLSMEKQRELKKQL
                     KNEARADVFVVKRRLLQKALSEEKIDFPEISGSVIAIFSNDEILPAKIIFNFKKEGKK
                     ERLEFIGGLLKDQFGYKILSSNELKELAVLPTKEEILASVVNILKAPMVKLNYALKGN
                     LQKLVYIFANVRE"
     gene            17512..17952
                     /gene="rplL"
                     /locus_tag="P5570_00095"
     CDS             17512..17952
                     /gene="rplL"
                     /locus_tag="P5570_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRR39161.1"
                     /translation="MAEKKEDQKEKKEIEIPENLKNIVKEIEELKVVDLAKLVKVLEE
                     KFDISAQAPMMAAPVAAGAASAGAAAEEEKTAFDLVLKNPGASKIAVIKIVKEITGKG
                     LKEAKDLVDASEKEPQTLMQGAKKEEAEETKKKLEEAGATVEIK"
     gene            complement(18032..20095)
                     /locus_tag="P5570_00100"
     CDS             complement(18032..20095)
                     /locus_tag="P5570_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39162.1"
                     /translation="MRIKELLFDVKELTVYCESFVYEPSNVEEEKLGHLFMLGRIRNS
                     DESSFYLINLLASRIKREYYNSLRRSANEAFELALKEANKVLKDNEDRINWLGNLDFF
                     VASVVQNRIYFTLLGKMRAFILRSNEVVDLVQNLIPERDVLFPFSTVIQGALKKDDVL
                     IFSTSNIFSKEKLLTQGAQLFPLEEKKLKELIETDESGVALVVETGKTAEVIERFTPA
                     LSPQKPLLKVPSLSPEKKEKIATNFQTISQNSKIFFKTTGEKIKNFFVKIPILIKKWR
                     KPTTPKQKETIPSITLEKVDTGIKFNWPKIKRTLLKKEVIIPFCFLIIGLIGFGIVQN
                     QKNFQISSAENTISTVEEKKQEAENKLIYGEKENALSLFGEALELLNTDEYLSWPAEQ
                     KEKADLLKKDLENKISELTGRKILSDISPLFEIKEGVEKWEPQGIIMVENNIYIFSNV
                     SNLVYKWDTKTKKEIFFPVNAEGVIGATVVNNVPIFLSNAKKITLENEKTFDLSFPYE
                     INLQNNPAIELDNFQNNFYIFDQSQGEIIKYPFSENEVGKSTLWFKERKAGKEGVSVA
                     IDGNIYLLQQNGIVKRFSAGSLKEEIASPNVFPKIKNATKIFTSPNNNYLYILEPEEK
                     RVIVTNKKGELIVEYQSEKFEDLIDLWVTPQDKKIYLLSINKKAEDQLSSTISQVFEI
                     EVETK"
     gene            20270..20878
                     /locus_tag="P5570_00105"
     CDS             20270..20878
                     /locus_tag="P5570_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39163.1"
                     /translation="MEELEILFGSKIRPRILKLFFQNEGEIFDAREIAGRCQINISTV
                     KKELEKLKKINLLQKKTRKEKSKTISGSSRSKYFYGLNPKFSLFSELRALILAGPVVP
                     LKDITILFKRESGVQLVVISGVLLKEEKAPVDVLIVSKKPKKAKLLKIIKKIESQAGK
                     EIRWAMMTVEEFHYRFEINDRFLKDIFSHNHKKIIDKLGVTL"
     gene            20879..20953
                     /locus_tag="P5570_00110"
     tRNA            20879..20953
                     /locus_tag="P5570_00110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20911..20913,aa:Val,seq:cac)
     gene            21235..21414
                     /locus_tag="P5570_00115"
     CDS             21235..21414
                     /locus_tag="P5570_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39164.1"
                     /translation="MDAITWVWVIVIVVVVGFIVYYLRQPEENKKEEKKEEPTEGNSQ
                     DDKTVPPSQNGTPNN"
     gene            21448..22785
                     /gene="aspS"
                     /locus_tag="P5570_00120"
     CDS             21448..22785
                     /gene="aspS"
                     /locus_tag="P5570_00120"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRR39165.1"
                     /translation="MQRTLIKNSPNLIGEKIKICGFVYSRRDHGGVVFLDVKDRSGVL
                     QVVCVPDLVTDVKDEYVVEIEGEIKKRPPKMSNSEIETGEIEMKAEKVKILNTAENLP
                     FDFKDLKCRLPKLLDYRPLTLRNDKIKAIFKVQEEIIASFRSVLKEMGFTQFESPAIV
                     PICAEGGAEVFHIDYFKNDAYLAQSPQLYKQILVPVFERVFTITKAYRAEPSITTRHL
                     TEYISLDAEMGFIDSWEELMDVCENVIKKIFLQIEKNCSKELKLHNASLPLIEKNIPR
                     LKMREAQEIISKRTGRNRRQELDLDPEDEKEICQFVKEKYQSDLVFITHYPTKKRPFY
                     TYPDPENSDYTLSFDLLFRGLEVVTGGKRIHQYQKLVDNIKKWGNKIEDFEFYLQAFK
                     YGMPPEGGFAFGAERVTKQILGLQNIREASLFPRDMTRIDTRLINNSSTSFKK"
     gene            23094..>25673
                     /locus_tag="P5570_00125"
     rRNA            23094..>25673
                     /locus_tag="P5570_00125"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         9051 a         3522 c         4961 g         8039 t
ORIGIN      
        1 cttatttctt aagtgcaatc ttaaactttg agtcctttct aaagattctg attttttgac
       61 tttttattaa agatatgtta atattttatt attaaagtaa aatttttatt tattatgttt
      121 gtcaattttg gatatattaa ttttcaaagc aaaaagtttg cacaagggca aactcaatta
      181 agagatttta aaaagggctc attctgagcc cttttgtgtt ggtttttaaa aagttattat
      241 aaaaattaat ccagctaaaa aaggcaactt ttaaaaatag attttctctg tttttaaaag
      301 atctgtcttt ctgtttaata atatgaattt ttcttttgat gtaaaaaaaa taagagatga
      361 ttttccagct cttaatcaaa aggtttatga taagcccctt gtttattttg acaatgcggc
      421 aaccactcaa aaacctaaag tagtaataga tattataaat aaatcatatt ccgaatataa
      481 tagcaatgtt catcgtggag ttcattattt aagtgaaaaa atgacagaaa gatatgaagc
      541 agcaagagag aaagtgcgaa attttttaaa tgctaaatat gctcacgaaa ttatttttac
      601 caaggggacc actgattcta ttaacgcagt ggcattttct tttggagaag catatgtaaa
      661 agagggtgat gaaattattg tttctgagat ggaacatcac agtaatattg ttccttggca
      721 gatgttgtgt caacgaaaag gagcttacct gaaggtgatt ccttttaatg atgacgggga
      781 gctattatta gatgaatata aaaaattaat taattcaaaa acaaaattag tggctgttgt
      841 gcacgcttca aattctcttg gcactgttaa tccggttaaa gaaattattt ctattgctca
      901 caaaaataat gttccggttt taatagatgg aactcaggcg atacagcata gaaaaattga
      961 tgttcaagat atggattgtg atttctattg tttttcagga cataaaatat atggaccaac
     1021 aggaatagga attttatatg ggaaagaaca ttggttaaaa gaaatgccgc catatcaagg
     1081 tggtggagat atgattgatc gggtaacgtt tgaaaaaact acttatgccc aactgcccgc
     1141 taaatttgaa gcaggtactc ctgattatat tggtgctgtc ggactgggaa ctgcacttga
     1201 ttatttaaac agtataggac tggataacct ttataaatac gaagaacaat tattgaagta
     1261 tggaacaaac aaattaaaag agatagatgg tttaaaaata tatggtatgg caaaagataa
     1321 aacttgtgtt ttttcttttt tattaaataa tattcctgct tatgacacag gaatgatttt
     1381 agacaaaatg ggtattgctg ttagaacagg gcatcattgc actcaaccta tttgggatca
     1441 ttacagggtt gaaggatcag caagggcatc tttggctttt tataatactt ttgaagaaat
     1501 agattatttt tgcgaaacat taaaacaagt tcaaaaaatg tttgaataaa aacatcaccg
     1561 gactaagcag cttgatgagt tttgataaac aaacataggt tcaaagattt aacgcaacac
     1621 cacaacggga ttgccatcgc gataataata aataagtctg tccttgcgag gagttcggga
     1681 cgacgaagca atctcgttac atattgttgc gttaaaaact tgattctacc aaacataaaa
     1741 tctgataaat ttatttaaac agaataaatt tatgcttatt ttgacccaaa aagaaatcaa
     1801 aaaaattctt cccttgaaaa agattgatat agttatcaat gctgtccaaa aagcattttc
     1861 tgattacggt caaaataaag ttcagatgcc cgctaaactc tatttatatt ttaaggagtt
     1921 taatggtgat ttaagaataa tgccttcttt ttcagaaaca ctgaaattgg caggaacaaa
     1981 gattgtaaat gttcatccag ataattataa aaagagattg aaatcaataa tggcagtgat
     2041 tgttttgaac gatgccagaa ccggtttgcc tttaacttta atggatggta cttatattac
     2101 tgctttgcga accggtgcgg cttcagccgt cgctaccaaa tatttagcta gaaaagaagc
     2161 aaaaagtttg ggggtgatag gtgctggttc gcaagcgatt actcaaattc ttgctattgc
     2221 tcaagtgaga aaattaagtg aaattttagt ttacgatatt aacgaagaag cgattaaaaa
     2281 tttagaaaaa attttggcca atgagaaaat taaaattaaa aaagcaagtt taaaggaaac
     2341 tgcccaaaaa gatattctgg taactatcac tccggctaaa aaaccaatta ttaaaaaaga
     2401 atggatttta cctggaactc atattaatgc gattggcgct gatgcagttg gtaaagaaga
     2461 attagatccc cagattttaa aaaaggcaaa aattatagtt gatgattggg cgcaagcaag
     2521 tcattcagga gagattaatg ttcctttgaa aaaagggata ttaaaaaaga aagatgttta
     2581 tgcttattta agcgagatta tttgtggcaa aaagatcggt cggaaaaata ataaagaaat
     2641 taccattttt gattcaactg gattggcagt acaagattta tatactgcca atatagttta
     2701 taatttagcc aagaagaaaa aactaggaaa acaaattaaa ataatttaat catatgacaa
     2761 aaaaacaaaa taatttatat tcagttttag agttaattgg caatactcct ttaattaaaa
     2821 taggagatat ttttgctaaa ttagaaacag ttaatcccag cggaagtgta aaagatagaa
     2881 tggcttggta tatgataaaa aaagcggagg aaagaaaaga attaaaaccg gggaataaaa
     2941 ttattgaggt caccagcggg aacacgggta tttcttttgc aatgatttct gctgtaaggg
     3001 gatataaatt tacagcaata atgccagaat caatgagtgt agaaagaaga aaaatgataa
     3061 aagcatttgg agcagaaatt tttttaacac cggctaaaaa agatatggct ggggcagtag
     3121 aacaatatca aaaactaata aaagcaaatc cagatgcttg gctacccaga caatttgaga
     3181 atccagataa tatagctgct catagagaag gtttggggaa agaaattata aaacaaacaa
     3241 aaggaaaaat agattctttt gttgctggtg ttggcacagg cggtacttta ataggcacag
     3301 cccaggcctt aaaaaaaatc aatcctaaaa taaaaattat tgctgtagaa ccagcagaat
     3361 ccgcaatttt atctggcaaa aaaccagggc tccataaaat tcagggaata ggcgagggtt
     3421 ttattccaaa attagttcag gaaaatatag ctattattga tgaggttctt atgattggta
     3481 gtaatgaagc aattgatact tcaaaagaac tggcagaaaa atttggaatt ttagttggaa
     3541 taagttcagg cgccaatgtt ttagcagcac gaagaataaa ggaaaagtat ggatttaaga
     3601 atatagttac ggtcttgcca gacagaggag aaagatattt aagtgaaatt ttttaatgat
     3661 aaatatgaca atagaaaaaa tacaaaatga aattattaaa gaatttgaaa atcttgatga
     3721 cgatcttaag aaatacaatt atttaattaa actgggaaga gaattaaagc cagttgattc
     3781 aaaatataaa acagataatt accttattag aggatgtcaa gtaaatacgt ggtatcattc
     3841 tgaatgcaaa gatggaaaaa tgttttataa tattgatagt gtttcattaa taacaagggg
     3901 gcttatttct ctgcttttaa gaatttgttc tgctcagacg ccagaagata taaaaaacac
     3961 tgaattttat tttattgata aaataggatt aaggaatcaa ttttcaccac ttaaagacaa
     4021 tagtcttttt aaattagtga ataaaataaa agcagatgct gatttttggt caaacaacac
     4081 aaaataattg ataaaaatat ggatgatatt aatcaaacaa aatgcccaat acccaattct
     4141 aaacattttt tggtttatga aagttctttg aagttttgca gaacatttta tgctgttttg
     4201 gttttgattt cttttttatt tcgtaatcag tggttactgt tggcaaccat tgttttaatg
     4261 tttctacgtc ttttttctgt taagcttgat cttccttatc aatttcatgc tttgttttta
     4321 agaaagttgc taaaagataa atcaaaacca gttatgaaag aaaaaagtga attgaacttt
     4381 gtttttggta tgatggcaat tcttctttta attggttttt tgcttattta ttttaataaa
     4441 tttgtcgatt ttgcttggat atttatttta atgatagatt tgttaatgtt tttagctgct
     4501 cttgttggct tttgcgtagc tactttaatg tatgtttttt tcaagaaaat ttttaaaaag
     4561 cagaaaaagt aaaactctga actaaaagaa ctaaaaatgg aaaaaagcga accatctcaa
     4621 aaaacaaaaa tagaattagg agaacctaat tatcttaata aaaattgttt attggctaat
     4681 tcccttggac attctcccta caaacgttgt cagtattgcg agtcaaattt ttctaattgt
     4741 atttttttac aatatcaaat aattagtttg ggtttgattg ttttttcttt tgttctttct
     4801 tttataatgg aaggaagaat ttcagaaacg ataatttttt ctgtttttgc tttagtgatt
     4861 gtttatggtt attttttcag caaaagcaca gataaaatta ttgaaaccaa ttttaaacgc
     4921 aaaaaagcag aggaattagc tttacgagaa aaaatttcaa aagaggaagc tgaaaaatta
     4981 gctgttcaag aaagagcgct aagaaaaaga acagagaagt tagttaacga gtataaaaat
     5041 ctagatgaag caaaaacaat gtttcttaat attgcttctc atcaattaag gactccgctt
     5101 tcagcaatga aaggatattt atcaatgatc ctagatggta attatccaag agaaaaagaa
     5161 aaagaattta taaaaactat ttatcgttct aatgaaagag aaatagagct tgttaacgat
     5221 cttttggacc ttaataaact tcaaacagga aaacttcaat ttaattttcg agatgatgtt
     5281 caattagaag atattattga agaaataatt aatgaatttc aagctgaagc tcaacataaa
     5341 ggtctttatc ttgtttttaa taagccaaag cagcccttgc ccaagattac agctgatcct
     5401 caaaagttac gtcaagttat tttaaatctt attgataatg ctttaaagta tacaagcaaa
     5461 ggtggagtta cagtaaatct taaaccttgc ccttcaagca ttcaaatttc tgttcaagat
     5521 acaggtatgg gtatgatgtc tcaggaaaaa gaaaaactct ttcaactgtt ctcaagatca
     5581 gaagaagcaa cctctgttta tccttctgga attggtatag ggctttacct ttcatcagaa
     5641 attgttaagg ctcataaagg caagatctgg gcagaatcac ccggaaaagg taagggttct
     5701 actttttatg ttgagttgcc agtaaagaaa taactaattt aaattaactt agtttagaat
     5761 tttacatata cacatttatt tttcttgggg ttatcttgct gaaaaattaa acctgtcaag
     5821 ataaataaat ttttataata gtattgaatt acaaatataa aatgtctata ataaacaaat
     5881 aggaagatta aatcagcaat aaaaattttc cattttgttt tttgattaaa gtttatgtcc
     5941 aaaaaagttt taattattga agacgaggaa gtgttagtag atatgtacaa agtagagttg
     6001 gagagattag ggtttaatgt taaatatgct ttagacggag aagatggtct tgaggcggct
     6061 aaagaagaaa gacctgattt aattattctt gatcttcttt tgccagggat gtcaggaatg
     6121 aaggttttaa ggaccctaaa agatgatcca gaaacaaaag atattccagt atttattttt
     6181 accaattatg aggcacccga agaaagagaa aaaggacaaa gattaggagc agaaaaatat
     6241 gttttaaaaa ccagtattac accacgagag gtaggagagt tgataaaaga gcgattggaa
     6301 aaaaataatt attaattata aacaaaagcc caaaaataga aaaaaagggt ttatttttaa
     6361 aatacagtaa ataaggttga catttttttt attttttgat atatataaaa agaataagtt
     6421 gttctttaac aattgaacaa ttgaattttt aagtagtttt gtcgaaccag tccacccgta
     6481 tgcttttggc aattgtcaat agcagacggg ttttataact gagaatttga tcttggttca
     6541 ggatgaacat tggcggcgtg gatgaggcat gcaagtcgaa cggttcagtt ttttcgcaag
     6601 aaagaactga atagtggcga acgggttagt aacacgtaga tacataccat agagtttggc
     6661 atagctcacc gaaaggtgag gtaattccaa atggtctgat agtcgattag ttatcgcaag
     6721 gatttaattc aaaattcaaa acgtaaaagt caaaataaaa gacgaaaaat tccaaatttt
     6781 gctttttgct ttttgctttt tgaatttaga gtgatttctt gtggtaagca atcggctatc
     6841 agtaaagatt tcgcaagaaa tcactctatg attggtctgc gtcctatcag cttgttggtg
     6901 aggtaatggc ccaccaaggc aatgacgggt agggggtgtg agagcacggc ccccaacagg
     6961 gaaactgaga cacggttccc actcctacgg gaggcagcag tcgagaatct tcgacaatgg
     7021 gcgaaagcct gatcgagcga cgccgcgtgc gggaagaagg tcttcggatt gtaaaccgct
     7081 tttaactggg agcaatccac ttttagtgga tgagagtacc agttgaataa gaggtgacta
     7141 aactcgtgcc agcagtagcg gtaatacgag tgcctcaaat gttatccgga attactgggc
     7201 gtaaagcgtc cgtaggtggt tttataagtt tcttgttaaa tcctttggct taaccaaaga
     7261 atcgcaagaa aaactatagg acttgagggt gttagaggtt ggtggaactc acggtgtagg
     7321 ggtaaaatcc gttgataccg tggggaacac cgaaagcgaa agcagccaac cagagcattc
     7381 ctgacactga aggacgaaag cgtgggtagc gaatgggatt agatacccca gtagtccacg
     7441 ccctaaacta tgctgactag ctatttggag tgtcgaccct ctaagtggcg aagctaacgc
     7501 gttaagtcag ccgtctggga agtacggccg caaggctaaa actcaaagga atagacgggg
     7561 gttcgcacaa gcggtggatc acgtggctta attcgacgac aagcgaagaa ccttaccagg
     7621 gcttgacatg ccagaatgta ctctaagaga aatcgaagag gaccgtcaat tcggaatctg
     7681 gcacaggtgc tgcatggttg tcgtcagctc gtgccctgag gtgtaccctt aagtggggta
     7741 acgagcgcaa ccctttccct atgttttaaa tgtcataggg gactgccttg caatagcaag
     7801 gaggaaggag gggatgacga caaatccgca tggcccttac gtcctgggct gcacacgtga
     7861 tacaatggcc ggtacaatgg gtttgccaag tcgtaagacg gagctaatct catcaaaacc
     7921 ggtctcagtt cggattgagg tctgcaactc gacctcatga agctggaatc gctagtaatc
     7981 gcggatcagc cacgccgcgg tgaatatgtc cccgaacctt gtactcacct taataaatgc
     8041 gcttggcagc ttttaaagct agatggttaa aatccattcc ttctggtagt ttgccagaaa
     8101 ggtagttgaa agataggtct tataccgcaa ggttaagact gaaggatctt aagacaaatg
     8161 atagcccaac caaatttaga aaagaggtaa tcgatctacg atttgggcgg tgaggtagag
     8221 aagattaatt taccgcctca gttgggatgt caggaaaagt tttaaaagtg acccaaggag
     8281 acccagtatt tttaaaagaa agcattgatg taaaaaaatt acatcgaaaa tactgggagt
     8341 cagctgagtt atagtacctg ccaaaatttt gaaaagtaaa atctaggcag ggaaggactc
     8401 tacctataat ttatgttatc tgcagattat atagttggat taactgatgg agagggtagt
     8461 tttactgtat ttatacatcc gtttcggaaa ggattaagca aatatcctcg gatagagtgt
     8521 cgctattata ttaaattaag agaagatgaa ttatccttgc tagaagaagt aaaaaatttc
     8581 tttggttgcg gaagtattta tttgcaaaaa gagaaaagat tgaaccatag aaactgttat
     8641 aggtttgaaa ttagcagtta tcaaaaaatt tctgaagtag ttatcccatt ttttcagaag
     8701 catcatcctc gtagcatagg aaggaaaaat gattttgatt tattctgtaa aatttttgaa
     8761 attgtttctg ataaaaaggg aaagcacata acggaaaaag agttaaagca gataaaagag
     8821 ttaaaattgc agatgcataa atatagggct ccgctgcgtg cgggaaatcc gcttgcgtag
     8881 tgggaacgca aaattagctt tcaattattg cattgcccgt cacaccaggg aagtcggcag
     8941 tacccaaagc ccctcaccta attagcgaag cgcttatgcg caaattaata aaatgtcgct
     9001 aattaggcga ggggttaagg taaggtcggc aaccaaggtg aagtcgtaac aaggcatcgg
     9061 taggggaacc tgtcgatgga ataaatcttt ttaaattttg tcgactattt ttaaaagctg
     9121 agaattctgc ttttaaaata aaaggagttg atcaaagatc cttaaagatt gatcacttgg
     9181 ggctggttcg acaaagctac ttaaaagcaa attttttaag tttgattttt taacaataat
     9241 ttgttaaaat tattagataa tttattaagt cagcgtatct taaactacgg caaatagata
     9301 tttatatcga aggcccttgt attaagaaag cgacttaagt aatgccgtgg taaattttta
     9361 ctacggagaa aggtcgccaa tataataagt ttgtagtttt ttaaatctat ataaagattc
     9421 taaaaagcta aaaagttaaa tttatggctt tcgataaaga aaggaatttt gaacgaaaaa
     9481 tgcatcaagg tgattgggct tgcagtgaat gcggagcaaa aattactgaa cttccatttg
     9541 agcctgctcc tgataggcct ctttattgca gggactgctg gaaaaagaaa agagacgagc
     9601 gttttcaacg ctaattccta aaaacaggat ttataactcc tcacccttaa aggtgggggg
     9661 tttatttttg agagaaaatt aagtaaaatt aattagaata tgttttaaag attaaaaatt
     9721 aaatattttt atgtccaagt tttatattac tactactttg ccttatataa acagtgatcc
     9781 tcatttgggg tttgcgctgg aaattgttca ggctgatgtg attgcgcgct attataggca
     9841 aaaaggatat gaggtatttt ttaacacagg cactgatgaa catggtttga agatttttcg
     9901 caaagcaaat gatcttggtt taagtcctaa ggaatactgc gatatttatt cagagaaatt
     9961 tagaaaatta aaagatgctc tctgtttaag ttataatagt tttattcgca ccactgattc
    10021 aaaacatatt aaggccgctc aggaattttg gaaaagatgt gctaaaaaca atgatattta
    10081 taagggaaaa tataaaataa aatattgtgt gggttgtgaa atggaaaaaa cagattctga
    10141 tttggtaaat ggaagatgtt ttcttcatcc caatcaagat ctggaagtgt ttgaggaaga
    10201 aaattatttt ttccgctatt caaaatacca aaaaaaactt ttaaatctat atcagaaaaa
    10261 tccacaattt gttttaccga aagaaagaat gaacgagatt attaatttta ccagaaaagg
    10321 gttaaaagat tttagcattt cacgagtgaa agaaaaaatg tcctggggtg ttccagtgcc
    10381 aggtgatgag gaacaagtaa tgtttgtttg gttcgatgct ttggttaatt atatttcttg
    10441 tcttggttgg ccagatgatt taaaaacatt caataatttt tggcctggtc ttcaagtggc
    10501 tggcaaagac aatttgcgcc agcagacatc aatgtggcaa gcaatgttaa tgtcagctgg
    10561 gttatcaccc tcccgtcaag tttttattca tggctttatt acctctaatg gtcagaaaat
    10621 gtcaaagagc ttagggaacg ttattaaccc ttttgattta gtgaataaat acagtactga
    10681 tgcagttcgt tattatcttt tgcgtgaaat tccacctagc gaagacgggg attttactta
    10741 tgaaaagttt gaaaaaagat ataatagtga cttagcagcg gggttgggca atctaacggc
    10801 aagagtgatg aagattgctg aaatttctca ttttcgattt tctctaattc ggcccgcaga
    10861 ggagtttaaa ataatttcta aacagtttca aaataaaaat tttcaaaaaa caatcagcga
    10921 tacttcaaaa aaatatcaat tatcattaga aaattttaag tttaatgaag ctttgatagc
    10981 agtatgggat ttgattggtt tttgtgataa atacattgag aaagagcgac cgtgggaaca
    11041 gaaagatcaa aaagttatta gtgatttatt atttgcgatt gaacaaataa gttatctgct
    11101 ccagccaatt ttaccccaaa cagcagataa aatattaaaa caattgaaga cccataaaag
    11161 tgaaatttta tttccaaaaa tatgaaaaag aaaattttta taggaagatt ccagccattt
    11221 cataaaggac acttagaggc tgtaaaatgg attgcaaaaa ataacggaga gattgttatt
    11281 gtaattggca gtgctcaaga atttgcaaca aaagagaatc ctttttcttt taacgaaaga
    11341 aaaacaatgg tgcaaaatgc tcttttaaaa gccagaatta aaaaattcag tatttttgga
    11401 gtgccggatt ttgttgagga tgatacattg tgggtaaaaa aagttttaga aataacaaag
    11461 tcaaagccag aagagataga agtttttacc cgaaatgcct ggacagaata ttgtttcaaa
    11521 aaattaggcg ctaaggtaac tcttcaccct gtatttttaa atggtttatc aggcacaatg
    11581 attaggaaaa aaattgctgc taatcaaaaa tggcagaatt tagtgcccga gccagtatta
    11641 aaatatttga aaaaaaataa gggtaaagag cgaattaaat cttacagcgt attaccagag
    11701 aaaaaaattg tttcttttat taaagacgaa atcaaaaatg ctggggcaag gggagcaata
    11761 atgggtatat ctggtggcat tgattcttcg gtcacagctt gtttagcaaa aaaggcgcta
    11821 agagaaaaaa ttgtttttgt ccaaatgcca ttttttaaga cctgtccctt aataaacaat
    11881 gcggatcttt taaaaaaaca tctttcaatt aacaccaaaa gggtttatct tggagatgtt
    11941 tttgagagtt ttcttggggt tttttcaaaa acaagcgaaa ttgctaaaga gaatcttaag
    12001 tcacgtttaa aaatgacaac cctttattat tttgctgaag aatcacaatt attggtaata
    12061 ggagcaataa ataaatcaga gttagaatca ggatttttta caaaatatgg agatggggga
    12121 gcagacattt tccccttagg agatttatac aaaactgaag taattgagat ggcaaaaaga
    12181 ttaaaattgc ctcagcaaat tattgaaaca atacctatca ttgattttgc aagggatcgc
    12241 ggcaaaaagc agtcgttaat aattccttat cagcggttag ataccatctt aaaattttta
    12301 gatcaaggta actttagtgt aaaagagatt tcctttttaa cagatgtttc ttttgaaata
    12361 gtaaaagaaa ttattaagag aaaaaagaat agccctgcta gaacaaatcc gcccattatt
    12421 tgtaagatca aaaattaaat ccaaaattat attaatttaa aaaagatatt ttaatgttta
    12481 atcaatttta aaatattctt tttttgtttt tatgaataaa aaaaatttaa ttattttaat
    12541 aatatcaatt agtttagcga tgacagggat ccttaaactt ataaacaaat cagacaatga
    12601 aagaaaagaa accaatagag aagtaaatca agatgaaaaa ttaccttatt tgccatcaga
    12661 aaaagaaagc tcaacaagtt cttctgaaga aacaaaagaa ttaatggcag taacaaaaac
    12721 gattgattct gatgctatta atcttactaa aatttccact caaaattgct atgcttttgc
    12781 tccagctggc tgggcaaaca tagaaagcat gcaatcagtc ggttttggtt atgatgcttt
    12841 tagtcccgat gaaacatcaa tggcttctta cggaatggtt ggcttgacag gtgactttgc
    12901 tactttttat ggtcttaatt ctgctgaaaa gtttattgag gttgatctta caaatctagg
    12961 atatactaat ttttcttggc ttagtgaggc gattgatgtt ggtaatgatt ataaaacaag
    13021 aacatttaag gcgattgagc cgcaaaaaaa cagaccagtt accggtattt ttttatatag
    13081 aacatgggat ctttcagatg gtggatttat tattgttctt cgatcagcta ttactacttc
    13141 tgataaatgg aaagaaaatt ctgctttggc ttttaatgtt tctctttcaa tgcgttgcac
    13201 agcaaaatta cagccctcta gttctccttc tgcttcttcc aatgatgaag tttcagatat
    13261 gattatagat tcttatgaaa aaagacagtc aactttagac gaagcaagca gtgaatggtc
    13321 agaaggaatg ctaggatatg aaaatgttta ttctcccagt acaggcgatc attattgggc
    13381 acccttggat tcaaaatggg agacaggacc atctggttct ggttattatc gtgtagtaaa
    13441 cccaaccaca ggcgatgtag aaaaattaga aaacggattt ggatattaaa aataaacaga
    13501 aggtggaaga tggaaaggga ttgcctcatt tctgatnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnattt ctgataactg atttctgttt aatctaatat taaaataaat
    13681 tagttttatg aataaaaagt ttgaaaagtt attagatgaa ttgaggaagt tagatttacc
    13741 cgatggaaaa tatgcaattt tgggaagtgg accattagcg gtaagaggaa ttcgagaagc
    13801 tagagatttg gatataatag tgacagatga tttgtataaa aaacttcaaa aagaatattc
    13861 tcaaaatgat aaaattaaat tagggaaaat agaaattttt tcagcaaatg gttggaggtc
    13921 taaatttgga gatttaacta aaatgataaa gcaagctgag aaaattcaag gattaaggtt
    13981 tgtatgcttg gaagatttga tagaatggaa aacaaaattg ggccgtccaa aagaccttga
    14041 agacataaaa ttaattaaag attatttaaa aagttttaat aatgtttttg gcgataatca
    14101 aagttagttt tttgttgggc attattacat ttttaagtaa gaatgtaata atgctgtaaa
    14161 atgaaaattg taatgatttt aaaactacta tggcattaaa aattaaaatt aaagatcctg
    14221 tgagcggact gagtcattta attggcgctg tgctgtctgt ttttggtaca gttattttaa
    14281 taagctcagc tgttcaagaa cagtcgtcta ttaaaataat ttctttttct gtttttggat
    14341 tgagtatgat ttttctttat gcctcgagca caatttatca tcttttcgga cattcaccag
    14401 aggaaataga tatttttaga aaaatagatc atgcaatgat ttacattttg attgcaggaa
    14461 cttatacacc aatttgtctt attcctcttt ggggcccatg gggatggtat tctctaattt
    14521 taatttgggc tttagctatt tttggtattt caatggtttt ttttaaaaaa ttctggacta
    14581 atgttcctcg gtgggtagct acagttattt atgttgtaat gggttggcta agcgttgttt
    14641 tgctttatcc gttaagccag aaggtttctg tttcctttat tggatggttg cttttaggag
    14701 gattttttta tacccttggt gcttatatat atgctcaaaa aaagcccaat ttttgtaaac
    14761 agtttggttt tcatgaaata tttcatatct taactatttt agggacgttt tgtcattttt
    14821 ggggaattta caactttatc atttaaattc taatttaatt aaaaaggcag gtttaagttt
    14881 ttaacgcaac aactctcttt tgtatttaac atgagctaag caatctcggg cgaagcttac
    14941 aactacacaa cggaatctct tccttttcac tttctattta ttatttaatt atttgtaaat
    15001 attgcattaa atttttgaac ctaagtaaat ttaaatatta atagaaaaaa attaaataaa
    15061 gactggctga tgaaaattaa aaatatgtta atagatactc atagtcattt aaattttagt
    15121 gattttgaag aagatcgaaa tgaggtgatt aaaagatgtt ttgaaaataa ggtttcaatg
    15181 attaatattg gtacgaattt tgaaacttca aaaaaagcga ttgaaatagc tcaacaatac
    15241 caaggaaagg cctgggcagc gatcggactc catcctatta atttggatac aggattgata
    15301 aaaatcaaaa atcaaaaatc aaaatgtaaa aattgggaag atttaacaga agataaattt
    15361 aattatcaaa aatacaagga acttagtaaa aatcaaagag tggtagcaat tggtgaagtg
    15421 gggttggatt attattggaa gccaaaaaca acaaagaaaa aagaaatatt taaaaaaaag
    15481 caaaaagatt tgctttttca agaattgaaa ttggcaagtg aattaaacct tccagtaatt
    15541 tttcattgtc gcatggccca tcaagatctt attgaaattt tattgtctca aaaatattta
    15601 tcatttaaaa aaagaagaaa aggagtgatg catggatttg ttggtaaaaa agaggaatta
    15661 gaagcatatc ttaagatggg tttttatatt ggttttaatg ggattatttt taaaaatatt
    15721 gaaggaatta attttataga aaatataaaa ataacacctt tggataaaat tttaatcgag
    15781 agtgattgtc cttttttaat gccgcctgtt tttacaaaaa gtgcaataaa accaggacgt
    15841 aatgagcccg cttctattag atttattgct gagaatattg ctgatataaa aagggtaagc
    15901 gtggaaaaag taataaatat tacaactcaa aatgcaaagc aattttttaa tatctagtat
    15961 tttgtttatt gcttttcaat ttaaattgtt ataaaataaa aatatggaac aagttgttgc
    16021 caaagaagca ataaagataa atattgataa gcccaaaata aaaaagagag tagtaatggt
    16081 tattgccttc cagggattta gagatgaaga atattttgtg ccaaaggaaa ttttggagag
    16141 tgccggtgtt gaagtaaaaa ccgcttctac caaattagga attgcattag gagatgacgg
    16201 aggggaagcg cgggttgatt ttttaataag cgaagtaaaa ccggaaaatt ttaatgcagt
    16261 gatttttgtt gggggacctg gttgtttgca gaatttagac aatgatgatt cttatcgttt
    16321 ggcaagagag acagtatttc aaaaaaagat tttagcagct atttgtattg ctcctgtgat
    16381 tttagcaagg tcaggagtat taaaagagaa gaaagcaaca gtttggacaa gtcctttaga
    16441 tacaacacct attaagattt taaaagacaa tggtgcagaa tatatagata aaaatgtggt
    16501 gagagatggt aagattatta ctgccaatgg tcccagcgct gctgaagaat tttctcaaaa
    16561 gatattagaa aatttataaa gtagtcgatg aagtcttcct attttggagt ctttcttcat
    16621 ctgtcgccct ttactttgat cgggcatttg acaaaagatt taaaatttta taatatacta
    16681 ttaccgtaga cagaaggcac aatcttgtgc tttggacaag tttgctgaac tgcactgatt
    16741 attttctgtg taattgcttg aaaatgacat atgttctaag atcttgttta aagtacaagg
    16801 tgataagtcc ttccgaggaa aaataattat tggaacacag gatataaata aaaataaaaa
    16861 ggtctgcggt aaaaatttcc gcagattttt ataataatta atttatgttg aaaaaagcac
    16921 aaaaaagaga acttgttaaa gaaattgaaa aaaaattaag agaaaataaa ttagctgttt
    16981 tttgtaattt tgagggacta tcaatggaga aacaaaggga attaaaaaaa caactaaaga
    17041 acgaagcaag agccgatgtt tttgttgtaa aaagaagatt gcttcaaaaa gcgctttcag
    17101 aagagaagat tgattttcca gaaatttcag ggtcagtaat agctattttt agcaatgatg
    17161 aaattttacc agcaaaaatt atttttaatt tcaaaaagga aggaaaaaaa gaacgattgg
    17221 aatttattgg tggcttgtta aaagatcaat ttggatataa gattttaagt tctaatgaat
    17281 taaaggaatt ggcagttctt cctacgaaag aagaaatttt ggcaagcgtg gtaaatattt
    17341 tgaaagcgcc gatggtaaaa ttaaattatg ctctgaaagg gaacttacaa aaattggtat
    17401 atatatttgc gaatgttcgc gaataaaaag gtccaaaaat ttaaaagtga aaagtagagg
    17461 tagaaagtag caaaagccgc gtttctacac tttacatttt taattaaact tatggcagag
    17521 aaaaaagaag atcaaaaaga aaaaaaagaa atagaaatac cagagaatct gaaaaatatt
    17581 gtaaaggaga tcgaggaatt aaaagtggta gatttggcaa aattggtaaa ggtgttggag
    17641 gaaaaatttg atatttcagc tcaagcacca atgatggccg ctccagttgc agcaggcgca
    17701 gcatcagcag gcgcagctgc cgaagaggaa aaaaccgctt ttgatctagt tcttaaaaat
    17761 cctggtgctt caaaaatcgc tgtgataaag atagtaaagg aaataacagg gaaaggttta
    17821 aaagaagcaa aggatttggt agatgctagt gagaaagaac ctcaaacact gatgcaagga
    17881 gcaaaaaagg aagaggcaga agagacaaag aaaaaacttg aagaagctgg tgcaacagtt
    17941 gaaataaaat aaaaagttaa aataaaaatt agtccgccta ttattttatc caatggcgga
    18001 ctaattttat ttttaaaagc aattcaaaat attattttgt ttcaacttct atctcaaata
    18061 cttgacttat agtagaagat aattgatctt ctgctttttt atttattgaa agcaaataaa
    18121 tctttttatc ttgaggggtt acccaaagat ctattagatc ttcaaacttt tcactttgat
    18181 attccacaat taattctcct tttttattgg taactattac tcttttttct tctggttcta
    18241 gaatataaag ataattattg tttggtgaag taaaaatctt tgttgcattt ttgattttag
    18301 gaaaaacgtt aggagaagca atctcttctt taagtgaccc cgcagagaat cttttgacaa
    18361 tgccattttg ttgtaataaa taaatgtttc catcaatagc aacagagacg ccttcttttc
    18421 cagcttttcg ttctttaaac caaagagtgc ttttgcccac ttcattttca gaaaatggat
    18481 atttgataat ttcaccttgg ctttgatcaa aaatataaaa gttattttga aaattatcta
    18541 attcaattgc tggattattt tgaagattta tttcataagg gaaagaaagg tcaaaagttt
    18601 tttcattttc aagagtaatt ttttttgcat ttgataaaaa gatcggtaca ttatttacta
    18661 ctgtagcgcc tattactcct tcagcattta ctggaaaaaa tatttctttt tttgtttttg
    18721 tatcccattt gtatactagg ttagaaacat ttgagaaaat ataaatatta ttttctacca
    18781 ttattatgcc ctgaggttcc catttttcaa ctccctcttt tatttcaaaa agaggagaaa
    18841 tatcagataa aatttttctt cctgttaatt cactaatttt gttttcaaga tcttttttca
    18901 ataaatcagc tttttctttt tgttctgcag gccacgataa atattcatct gtatttaata
    18961 gttccagtgc ctctccaaat aaagacagcg cattttcttt ttctccgtaa attaatttat
    19021 tttctgcttc ctgttttttt tcttctacgg ttgagatggt gttttcagca gaagagattt
    19081 ggaaattttt ttgattttgg acaattccaa acccaataag tccaataatt aaaaaacaaa
    19141 atggaattat cacttctttt tttaaaagag ttcttttaat ctttggccaa ttaaatttta
    19201 taccggtatc tactttttca agagtaatgc tgggtatggt ttctttttgc ttgggcgttg
    19261 tgggcttgcg ccattttttt atcaatatag gaatctttac aaagaaattt tttatttttt
    19321 caccagttgt tttaaaaaat attttggagt tctgagaaat ggtttgaaaa ttagtagcaa
    19381 ttttttcttt cttttcagga gagagagaag gcacctttaa aagaggtttt tgaggagaaa
    19441 gggctggagt aaatctttca atgacttcag ctgttttgcc tgtttctacc accaaggcaa
    19501 ccccactttc atcagtttca atcagttcct tcaatttttt ttcttcaagt ggaaataatt
    19561 gggcgccctg agtaagaagt ttttctttgg aaaaaatatt agaagtggaa aagattaaaa
    19621 catcatcctt tttaagtgct ccctgaatta ctgtagagaa cggaaataaa acatctcttt
    19681 cgggaattaa attttgaact agatctacta cttcattaga ccgcaaaata aaagcccgca
    19741 tttttcccaa tagagtaaaa tagatgcgat tttgcactac actggctaca aaaaaatcaa
    19801 gattgcccaa ccaattaatt ctatcctcgt tgtcttttaa aaccttattg gcttctttaa
    19861 gcgctagttc aaaggcctcg ttggcagaac gacgaagtga gttgtagtat tctcttttaa
    19921 tgcgtgaagc taaaagattt attaaataga aagatgattc atcgctattt cttattctgc
    19981 caagcataaa aagatggcct agtttttctt cttcaacatt gcttggttcg taaacaaagc
    20041 tttcacaata aacagtgagt tcttttacgt caaacaagag ttctttaatg cgcataaata
    20101 ttattttaaa gttaaaattt tagatttatt tttattattc tataataact ctttttacag
    20161 gttttgacaa tatgtaagtt tttttttatt attatatata gaaaataata aattttataa
    20221 tgtttaaaaa tttaatataa aaatttatta aatttttaaa agaaaatata tggaagaatt
    20281 agaaatatta tttggttcaa aaataagacc tcgcattctt aaattatttt ttcaaaatga
    20341 aggagaaatt tttgacgcaa gggaaattgc aggaagatgt caaatcaata ttagcacagt
    20401 taaaaaagaa ttggaaaaat taaaaaaaat taatcttctg caaaaaaaga cccggaagga
    20461 aaaatcaaaa acaatctcag gatcatcgcg gagtaaatat ttttatggtt taaatccaaa
    20521 attttctctt ttttcagaat tgcgagcatt gattttagca ggacctgtag ttcctttaaa
    20581 agacattact atactcttta aaagagaatc aggggtgcaa ttagtagtga taagtggtgt
    20641 tcttttaaaa gaagaaaagg caccggtgga tgttttaatt gtaagtaaaa aaccaaaaaa
    20701 ggcaaaactg ctcaaaataa taaaaaaaat tgaaagtcaa gcaggcaaag aaatcagatg
    20761 ggcaatgatg acggtagaag aatttcatta tcggtttgaa atcaatgatc gattcttaaa
    20821 agatattttt tcccataatc ataaaaaaat tattgataaa ttaggagtta ctctttaagg
    20881 acggttggct cagcggtaga gcgcttcgtt cacatcgaag aggtcgcagg ttcgatccct
    20941 gcaccgtcca ccaaaaagag tttgcaaatt tttggacaag gaaaaaatgg tttacttgtt
    21001 gtgcttgcgg gcttaacaga aaagaaagaa gctgtaaaat taattgatgg gatataatct
    21061 taagctgttt taaaaagggt tcagcatttt taattataca tatcaatttt aaaaaatcct
    21121 tttctgtgtt gaaaaggaaa aaggtcaaaa ttttaaaaag gagtttctaa attttaatta
    21181 atgagtttaa aacgtaaaag atggatttga tgtttaaagc tccttttgta atatatggat
    21241 gcaataacat gggtttgggt tattgttatt gttgtcgtgg tgggttttat tgtttattat
    21301 ctgaggcagc cggaagaaaa caaaaaagag gagaaaaaag aagagccaac agagggaaat
    21361 tctcaagatg ataaaacagt accgcccagt caaaacggaa caccaaacaa ttaatattaa
    21421 tagatagttt tttattcttt tgtatttatg caaagaacgc ttataaaaaa ttctccaaat
    21481 ttaattggag aaaaaattaa aatttgcggt tttgtttact ctcgcagaga tcatggcgga
    21541 gttgtttttt tagatgtaaa agatcggtct ggtgtcttac aagtagtttg tgtgcctgac
    21601 ttggtgacag acgtgaaaga tgagtatgtg gtggaaatag aaggtgaaat aaaaaaaaga
    21661 ccgcccaaaa tgtcaaattc cgaaatagaa accggtgaga tagaaatgaa agctgaaaaa
    21721 gtaaaaattt taaatactgc tgaaaattta ccatttgatt ttaaagacct taaatgcaga
    21781 ttgccaaaat tattagatta taggccattg accttaagga atgataagat aaaagctatt
    21841 tttaaagtcc aagaggagat tattgcaagt tttcgtagtg ttcttaagga aatgggattt
    21901 actcaatttg aatcaccggc tattgtgccc atttgtgctg aaggaggagc tgaagtattt
    21961 catatcgatt attttaaaaa tgatgcttat ttagcacaaa gcccacaact ttataaacaa
    22021 atattagtac cggtgtttga aagagttttt acaattacaa aggcatatcg ggcagaaccc
    22081 agtattacta cacgtcattt gacagaatat attagtttag atgctgaaat gggatttatt
    22141 gattcttggg aagaattaat ggatgtttgc gaaaacgtga ttaaaaaaat attccttcaa
    22201 attgagaaaa attgctcaaa agaattgaaa ttacataatg cctccttacc tttaatagaa
    22261 aaaaatattc ctcgtttaaa aatgcgtgaa gcccaagaaa ttatttcaaa aaggaccggt
    22321 agaaaccggc gacaagaatt ggatctagat ccagaggacg aaaaagaaat ttgtcaattt
    22381 gtaaaagaaa aatatcaatc agatttggtt tttattactc attatccaac aaaaaagcga
    22441 ccgttttata cttatcctga tcctgaaaat tctgattata ccttaagttt tgatttgctt
    22501 tttcgaggat tagaagtggt gactggcgga aaaagaatcc atcaatacca aaaattagtt
    22561 gataatataa aaaaatgggg aaataagata gaggattttg aattctattt acaggcattt
    22621 aaatatggta tgccacctga gggtggattt gcttttggcg cagaacgagt aacaaaacaa
    22681 atattaggcc ttcagaatat tcgcgaagct agtttatttc ctcgggacat gacaagaatt
    22741 gatacacgtc ttattaataa ttcttcaact tcgttcaaga aataacaatg ttgttatttc
    22801 ttatctttta ggagttatag gggggtcttg acttttttta ttttttttat agaataatag
    22861 tagaatgtat aaggttatgt ataataagaa ttgttttttt atatttggaa gtggctaata
    22921 tgcctgtgct ttaaattatc ttgaaaagcc gcttccaagc ggttttttaa atataaaaaa
    22981 agaaatgatt gagaaggata gttctttgac aatttaagag gaaaaaaata agttcttcaa
    23041 acattaaaag taggaagatg aatttatttt cattaacttg ctttttgatt tggagactac
    23101 agaataaggg tctatggagg atgccttggg agattaaggc gatgaaggac gtggcgtgac
    23161 tgcgataagc ctcggggagg tgtttagcaa cctttgatcc ggggatttcc gaatggggaa
    23221 acccagcact gcgaaaagca gtgctaccgc accgtttcgc agtgaattca aaatgcaaaa
    23281 gtcaaaatgc aaaattaagg tataaaattt tctaaaagaa aattttatga tttctatttt
    23341 tttgaaattt aatttgctgt gtgcgaacag tggacatttt tgatttttga tatttgattt
    23401 ttgattttac cgcgaagcgg tgtggggcac acccgctgaa gtgaaacatc ttagtaagcg
    23461 gaggaaaaga aaacaatctt gcgcttcgca caaggaaatt tcaaaaaaac acaaagcacc
    23521 aaatcacaaa attcaaaact gttttgagtt ttagtcattg gaatttaaga tttgtttgtg
    23581 atttggaatt tgtgatttga gattttctcg cgcggagcgc gaggacattc cctgagtagc
    23641 ggcgagcgaa aagggaggag cccaaacctg gccgtacctc actgcgtttg gcacggaatt
    23701 tacaaatttt caaacaaaaa tttttagaaa tttaaaattg aaaattagaa attccgcccg
    23761 agcatagcga ggtgcggctg ggggttgtaa ggagatggta tcagaacttt ctcaattttt
    23821 taaattgaga aattgctgag gaagaatatt taggtaaagt tattaatcga atgttcctgg
    23881 aaaggacaac catagaaggt aaaagtccag tagataaaaa taactttatg cttcttgcca
    23941 tctttcttga gtacctcggg aaacgaaagc cccgagggaa tctggctgga ctatcagcta
    24001 aggctaaata cttaatctca ccgatagtga actagtaccg tgagggaaag gtgaaaagca
    24061 gggaggtgat cccgttgaaa tagaacctga aaccatagac ctacaaagag acggagcctt
    24121 gtgcttcaca caagcaaact ctaaacttaa aaccctaaac tctaaagctg aatctttttt
    24181 aagtaaaata aagcgatcaa agaacgaagt tcctttcttc tctcaaaaaa gtttttgagt
    24241 tttgaaattt gaactttgaa attatttaga gtttagaaat tagagttttc ttgcgtggag
    24301 cgcgaggtga cgtcgtgctt tttgcataac gagccaacga gtttttttgt cagtttgtct
    24361 aagcccgttt tagggtgtag gcatagagca atcaagtgtg aaattgcgcg attaataact
    24421 ggcaaataaa gacccgaagc cagacgagct taccatgggc agggtgaact ctgtagaaat
    24481 acagagagag gcccgaaccc gtccgcgctg caaaacggtg ggatgacctg tggtaaggag
    24541 tgaaaagcta atcgagtctg gtaatagctg gttctcctcg aaatagcttt agggctagcg
    24601 cctattatgt tttctggagg tagagcactg gaaagaatac tgtgccttcg ggtaggtatt
    24661 cttaccaaac tccgaatgct ggaaaattaa aataggtagt tagactgcgg gggctaagct
    24721 ccgtagtcaa aagggaaaaa gcccagaccg tcatctaagg tccctaaatt tttgttaagt
    24781 ggaaaaagaa gtttctctgc taagacagat agaaggttgg ctcagaggta gccatccttt
    24841 aaagagtgtg taacaactca ctattcgact gccaaatctc atttatgaga cgaggcgcga
    24901 gaagagaact tgttctcttt gagggcagag gagcgctgaa gatttaacgg ggctcaaaca
    24961 aaataccgaa gatacggatc cttgtgcttc gcacaaggat ggtagaggag cgttttcttt
    25021 gcgttgaagc caaagcgtga gctttggtgg agcgaaggga agtgagaatg ttggcatgag
    25081 taaccgcaac tctgctgaga aagcagagca ccgaaaatct aaggtttcct tggcaatgga
    25141 tatcaaccaa gggttaggcg atcctaactt cgtcactgaa aagtgtcggg ggatgggtag
    25201 tcggttaata ttccgaccct tctttgctaa ttctgaagga gcaacgaagt gaagtagttt
    25261 gagcggatta ttggatttcc gttctttgct taagaaggtc ttattggcaa atccgtaaga
    25321 cagtttctca tttcgatgag gaacaacttc aaaagttgag aaaattcaga attgtctgaa
    25381 aattcaaatg agctcgcttc cgagaaaaac ttcgtaggat ttattagcaa agtattcgta
    25441 ccgcaaacca acacaggtag atgggtgtaa aagcaccaag gtgaacgggt gattcctcat
    25501 taaggaactc ggcaaaaaag tggtcgtaag ttcgcaataa gacctacctg ccttgtgctt
    25561 cgcacaagga aattccaagc accaagtttc aaacaatctc caagcactaa aattcaaaac
    25621 tgttttgaat tttagtcatt ggaatttaag atttgtttgt tatttggaat ttg
//
LOCUS       DAOTQD010000002        24023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_2182_length_24023_cov_7.428154, whole genome shotgun sequence.
ACCESSION   DAOTQD010000002 DAOTQD010000000
VERSION     DAOTQD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 24023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24023
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2182_length_24023_cov_7.428154"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="P5570_00130"
     CDS             complement(<1..260)
                     /locus_tag="P5570_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39166.1"
                     /translation="MPQNKLRIKDLPKEDRPREKLISKGPQNLKDEELLAILLGTGRE
                     GKSALDLAKEVLRKYSKKRLFKMNYQDLTKIKGISSAKACTI"
     gene            complement(532..1722)
                     /gene="eno"
                     /locus_tag="P5570_00135"
     CDS             complement(532..1722)
                     /gene="eno"
                     /locus_tag="P5570_00135"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRR39167.1"
                     /translation="MEKIKRIKAIEILNSRSNTTVKIEIASDNFTATAAVPEGKSVGK
                     YEALSLPAWKAIKNVEEKIAPLLIGKEVNFQLADKTMIELDGTRDKSNLGVNAILGVS
                     LAIAKLQAKIEQKPLFEFLSQFFLKGYRPGDTDFPFLLMNIVNGGIHAFGGPDIQEYL
                     IIVKGDSLKDGVFLGARIYQEMGKFFKGNIGDEGGFVPQTSNVQLPIEAISQTVSEKL
                     VRNKIKIGLDLAASQLFDGKKYNIGEDHFSPEQLIDLYLRWANRFEIIYLEDPFSEND
                     PNSFTQLKKQISNQTLVVGDDLTVTNIERLKEAKEKDLISGIIVKPNQIGTLSETIEV
                     INFAQKNDIKVIISHRSGETNDTFIADLAIASNAWGLKSGAPARGERVAKYNRLLEIE
                     EIKK"
     gene            complement(1754..3901)
                     /locus_tag="P5570_00140"
     CDS             complement(1754..3901)
                     /locus_tag="P5570_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRR39168.1"
                     /translation="MKKKKKNKNQPQLRKKNSQSRFFNLSPEAKKSIWGVIFAFVGIF
                     FVFSLLGIGGEAGNSLSKVLYLFFGNGAWLIAIIFFAIGIIFFLSERPSPYPSTISGG
                     ILFFIAIFSLLELCSEDAGGFLGKTLISLPQKYFGVPGSFAFFIALLLISISLLFNIS
                     VFKKVKIFSSKQKKEEKDLSFEELKKLQEGKAKIKVKEILPQKISKEKIEKTEEPIIK
                     KVKKVDYKTLPLALLDREENNAIAGNIRSNAQIIKRTFHNFGIDVEMGEVSIGPSVTQ
                     YTLKPAEGIKLSRILSLQNDLALALAAHPIRIEAPIPGRSLVGIEVPNQKRAQVRLAN
                     LLSSPEFLFLPPLGIALGKDVMGKPYFGDIGSMPHLLVAGATGSGKTVFLNSLILSLL
                     WRNGPQDLRLALVDPKRVEFSPYSSLPHLLCDPITQHQKIIPLLKWLTKEMEDRFSIL
                     QNAKKRDIKSYNEFVEIQIAKGNEEFEKLPYIVLIIDELADIMSSKGKEFEAVIVRLA
                     QMSRAVGIHLVLATQRPSVEVLTGLIKANITSRVAFKVASQIDSRTILDMAGAERLLG
                     QGDMLYLSGEAAKPKRLQSAFVSSEEIRRVVDFVIKHQQEEKTQKLTESLEETLNNET
                     VESDLFQGGPGGDDELYEEAKRVVMEAGKASASLLQRRLKIGYARAARLLDMLEENGI
                     IGPSQGAKPRDVYLKEDSKENNNSEEEDLNFPN"
     gene            complement(3879..7436)
                     /gene="rpoC"
                     /locus_tag="P5570_00145"
     CDS             complement(3879..7436)
                     /gene="rpoC"
                     /locus_tag="P5570_00145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRR39169.1"
                     /translation="MSIVEDFDAIKIRLASPKEILSWSHGEVTKPETINYRTQRPEKD
                     GLFCEKIFGPTRDYECYCGKYRKIRYKGVVCDKCGVEVTKSSVRRERLGHIKLAVPVA
                     HIWFLRGVPSRIGLLLGKSMQQVERVVYFMSYIIVEVDEESKKKIILQIKEEFERKKK
                     GKSKEEKQALKEELQKAKEEIASIKKGTIISENDYRRLSLKYGECFWASTGAEAIKKL
                     LESIDLKKLEAESKEELEKTSTKKYATQRLRLVRSLIRSGAKPEWMCFTYLPVLPPDL
                     RPMVQLDGGRYASSDLNDLYRRVINRNNRLKYLKEIGAPEVIVRNEKRMLQEAVDALI
                     DNSSRKTQMVQASTGQRRQLKSLADMLRGKQGRFRQNLLGKRVDYSGRSVIVVGPELD
                     IEEVGLPKKMALELFKPFIINKIIERGLAHNIRAAGILISEGRSEVWEILEEVIRGKY
                     VLLNRAPTLHRLSIQAFRPVLIEDQALHVHPLICPAFNADFDGDQMAVHLPLSKKAQA
                     EARNLMISSKGVLKPATGQPIAIPRQEMVLGCYFLTTVRPGARGEGRAFSSEEEAKIA
                     YNLDQIDLQAVIKVRIKKENNKLIETSVGRIIFNEALPKDFSYQNKVYNSKTIQKLIS
                     ELVGHYEQRTIISILDNLKNLGFEYATISCSTWGMDDLIVPKEKPDLLKEAALKEEEI
                     NKQYEMGLLSETERKVKIVEVWQGVKDKISKLVPSTLPQFGPVFSIIDSGSRGSWSQP
                     SQMAGMKGLVASPSGEIIELPVKNSYKEGLTTLEYFISTHGARKGTADTALRTSSAGY
                     LTRRLVDASHELIIREKDCGDKKGIVIYKKQAEEIGQELSQKILGRIILQDIKDGEKI
                     ILKKGDLISKEAADFIEKSKDIQEIRVRSPLSCKSKEGICQMCYGSDLTTHQLVNLGE
                     TIGVIAAQAIGEPGTQLTMRTFHTGGVAGGGDITLGLPRVEELFETRPPKGETVIFEE
                     KGKIEKIQEKKDGFSIIVKTEKGDQKEYSVPVKTELLVKKGDDVFPGMPIFKGRINLK
                     GLFKVAGIDETQRYILKEVQKIYTGEGVAIHDKHIETIIYQMFSRLKITKAGDSNFSR
                     GEIVSKAAFFEENDRLRRKGKEEAKGKIILLGLTQIALTTDSFLSAASFQETSRVLIQ
                     AAVEGKVDKLRGLKENVIIGKLIPAGTGFKALHEEKKEK"
     gene            complement(7474..10641)
                     /locus_tag="P5570_00150"
     CDS             complement(7474..10641)
                     /locus_tag="P5570_00150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRR39170.1"
                     /translation="MEKNIFSKYSFQFPYPNLIRFQIDSYQEFLEKRLNNLLKDFSPI
                     KDYSGKEFELYFDGFKLQEPKMDADKAKENNASYEASLFVQARLKIRSQGEERKGEVY
                     LCNFPLITKSGSFILNGVERVIVSQLIRSPGVFFTANYFYGKKLFGAKLIPNRGAWLE
                     FETDKNGVLWVKIDRKRRVLATTLLRAFSPLSNAEIKELFEENLKECEINYIEETLQK
                     DKAKNQDEAIMEIYNHLRPGDLVTVENARGFFEGLFFNPQRYDLSEVGRWRIQQRMRL
                     PEVKNITAKDRLLNPQDIIVVLKEIIELNKNPHAQPDNIDHLGNRRIRTLAELLESRL
                     RVGMLRMERIIKDKMSTIDASEAEPKNIINPHPFASQVKEFFTLSQLSQFMDNENPLA
                     EVEHKRRLSALGPGGLTRERAGFEVRDVQPSHYGRICPIQTPEGQNVGIITHLAAFSR
                     INRLGFLETPYFKVEKGKVTNKVDYLNGYEEEKYNIAPATTPRNANGKIIAQKIEARI
                     NGQPGVIETDKIDYIDVSPQQILSMAASLIPFLEHNDANRALMGCNMQKQSVPCLRPE
                     PPLVATGAEKKVAENSGQLIFAEEDGKVVKVDASHIAIETKRGEIKEYPLLNFIKSNQ
                     YTSISQRPVVEIGNKVKKGDLLAEGVAVANGELSLGKNLLCAFLPWRGANYEDAIILS
                     ERVAKQDVFTSLHIEDFMCDVRETKLGPETTTADIPNVSEAKLSNLDKDGIIRIGAEV
                     EAGDILVGKVSPKGEEDLSSEEMLLKKIFGEEAKEVKDTSLTLPHGKRGKVLRVKVFE
                     RNKGYKLEPGVIKRIQVEVAYLRKISVGDKLAGRHGNKGVIAKILPEEEMPFLEDGTP
                     IDIILNPLSVASRMNIGQILETHLAFAAKKLGYQAVSPAFDGATEQDIKRELKKAGLP
                     ENGKVKLFDGKTGEPFREKITVGYMYVMKLIHMVEDKMHMRSIGPYSLITQQPLGGKA
                     QFGGQRFGEMEVWALEGYGAAYTLQEMLTIKSDDVIGRSTTYEAIVKGEEIKTSHLPS
                     TFNVLLAELKSLGLNVELIKREGRK"
     gene            complement(10907..11473)
                     /locus_tag="P5570_00155"
     CDS             complement(10907..11473)
                     /locus_tag="P5570_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39171.1"
                     /translation="METKLLAYAVFVKKNGKVLFCEELFRPFVSLPGGEVKIPRVARE
                     NDHYCLCGLGSAMNPVLPEFFEAERDWVNKELSKIVESQAGMRIPTDDFFPVYPIVDQ
                     KGLVLFYPILVEENPSKKNVKWFFFKNIEIFAQEKKVSKLDYFMTIFSLATLKNDFQK
                     EAKKILEKTQEEEKKNYKRKMKEFVKNF"
     gene            11573..11686
                     /locus_tag="P5570_00160"
     CDS             11573..11686
                     /locus_tag="P5570_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRR39172.1"
                     /translation="MVVGDKEMEHKSVRVRERGKGDIGEIKLEKFLEKIKK"
     gene            complement(11725..12357)
                     /locus_tag="P5570_00165"
     CDS             complement(11725..12357)
                     /locus_tag="P5570_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39173.1"
                     /translation="MIIIVGGFSSTGSKRLKTLEEEIKKNFPEEEVLVPEYFERYGKL
                     RKYLRTKTIPEYAWNVQRETLFINNDEPILIGYSMGGIITRFLVEKIGLKAKEVILVG
                     APNRGVKINFVEDKLVGKLVSLPCVREIQPESNFLRLLNWNWHPEGYYFIGSDRDERV
                     PINSAIPMKEEGKFFILHTSHSGLIPKKAEMVPKSAIPIIIEILKNEIQT"
     gene            complement(12590..12674)
                     /locus_tag="P5570_00170"
     tRNA            complement(12590..12674)
                     /locus_tag="P5570_00170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12638..12640),aa:Leu,seq:caa)
     gene            12794..13558
                     /locus_tag="P5570_00175"
     CDS             12794..13558
                     /locus_tag="P5570_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR39174.1"
                     /translation="MTRVISIINEKGGSGKTTTAVSLGAFLTKNGKKVLLCDLDPQGN
                     ATTSLGLDLKHLSLNFYHALSQQVPLKDIIKPTSLFNFDIAPASADLAGSNIELLDKE
                     NRENILSNILSPLKEDYDFILLDPPPSLGILTINALIAADEVIVPIQCEFFALKSLDE
                     LFEIFNLLKRNLNKEFSQIFGLLTMYDKRNILSREIAKEAEKKFPGKMLETVIPRSIR
                     LAEAPKYGKTILQYAPESKGARAYESLAEEIINFKI"
     gene            13601..14437
                     /locus_tag="P5570_00180"
     CDS             13601..14437
                     /locus_tag="P5570_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRR39175.1"
                     /translation="MSGLESLIPKKINKSTSQKKESVFWIEIDKIKTNPFQPRKEFNQ
                     KELKELADSIEKYGILQPLIVRKIEKDVPNGQKVEYQLIAGERRLRASKMIGLKEVPV
                     IVKEISKENELPISLVENIQREDLNPLEKALAFKKLIEEFHLTQKEVAKMVGKSRESI
                     ANTLRLLILPEEIQKGIQRGEISEGHARALLSIPKEKQIDVYKETIKKKLPVRELEQK
                     AESKKEKKEILNLKKLQDEIFQVFKINNFKIREKNNKIELTLNFNSEKELRNFLKEIK
                     PS"
     gene            complement(14424..18083)
                     /locus_tag="P5570_00185"
     CDS             complement(14424..18083)
                     /locus_tag="P5570_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRR39176.1"
                     /translation="MKTSEKEKEILKFWKKEKIFEKGLQKSKKNPKFIFYEGPPGANG
                     RPGLHHVLSRSYKDIICRYKAMKGFYVERRAGWDTHGLPVELEVEKQLQIKTKQDIEK
                     LGIDKFVENCKNLVWRYKTEWEKMTEEIGFWLDFDNAYITYDPLYIESLWYTIKEIYK
                     KGLLYEDEKIVPWCPRCQTSLSSHEVSLGYQKVKDPAIYIKLKLKNKDSKGNKFNTQQ
                     NQSEARNKNKIFNNHSKSNIYFLAWTTTPWTLPGNMALAVNSKIDYVLMENEGEQFIL
                     AKERIKYLFKDESKFHIIKEFKGKDLVGLEYEPLYQTPNLDFQTPNQSKIYYVVAGDF
                     VSTDEGTGIVHIAPAFGEDDMAVWRQQNSNLKFSIFKPVDEQGKMKTPGYKWDKLFVK
                     DADPLIIGDLKNRNLLLKEELYEHEYPFCWRCKNPLLYYLHSSWFFATEKIKEKLIKN
                     NQKVNWVPPFIKEGRFGKWLEEIQDWNFSRERYWGTPLPIWKCNNCNNIEVIGSREDI
                     RKQKFSTNRYFILRHGLAQQNVKGILSSSLQNKFHLTNIGKLEVKKSVLKLKKLLGKE
                     KLDIIFSSDIQRCKETSEIVSRELGTKVIFSSKLRELNFGEYDGSTVEKYRNDFPFSI
                     LRFEQRLPKGESWNDCKLRMLKFINNVEKKYQNKNILIVSHGDPLWLLESVMKGLNNE
                     EILKSRDKIMMSKGELREIEMKKFPYNKKGELDFHRPFVDEIEFYCPNCKRKKMQRVK
                     EVGDVWFDSGAMPFAQNYWPFSQPKNKKSKPPELFPANFICEGIDQTRGWFYTLLAVS
                     SLLGFSTPYKNVLVLGLVLDKKGKKMSKSKGNAVFPEEMINKYGADAIRFYFFTVNQP
                     WEGKRFDEKDLRKTYNRFILTVENLLNFLLLYTKKSKASSTEIKNILDRWILSKTENL
                     KKDVDQLLSNYDVVKAARLIENFVVDDLSNWYLRRSRERLKKGDRGTIFTFNYVLLNL
                     SLLLAPFTPFLSEEIYQKVKGFNFERKKSVHLENFPQIKKEFLNKNLEEKMNEVKEIA
                     ALGLAERKKSGIKVRQPIASLKIKNQISSFATTKRDAQRILFFSRLGRSFNGRREESS
                     LRPSKIKNNEGLLNLIKDELNVKEVIFDSNINGKVELDTKITPELKNEGILREILHQI
                     QEMRKSVKLKPKDKIQIIYDFPKDFAVIFKKNEELIKNFTLAKEITTTKKPPYLIERQ
                     LNEDGKVFWLAIKKV"
     gene            complement(18114..18743)
                     /locus_tag="P5570_00190"
     CDS             complement(18114..18743)
                     /locus_tag="P5570_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39177.1"
                     /translation="MKNASFSQILIIVIVSIIIIGGGIFTWQYFGTTKQEIKAPEEMV
                     ESEEVSAPEEVSEPEGTVSEPEETIKNGTTNWQIYRNEEYGFELTFPERWQGYKVVEE
                     DSVWGIETISFQLEREESLVSDNEYYGSIFKITILSKDQWKALQQEEGPTPAYITEND
                     KYVFTISMCQDDMGFKGFPEDPGPGRFYEGPIYDVKTLILPSFKLIEAE"
     gene            complement(18758..21172)
                     /locus_tag="P5570_00195"
     CDS             complement(18758..21172)
                     /locus_tag="P5570_00195"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRR39178.1"
                     /translation="MQKQYNPQKVEKEIYSFWKKEKIYKFNPKKKGSFFAVDTPPPTV
                     SGKMHLGHAFSYSQQDFIVRYHRLKGENIFYPFGTDDNGLPTEKLVEKLNNVKIFDFE
                     REEFTHLCQKTLREIRPDFVKDWRKIGISSDFDLCYSTIDKNVQRLSQRFFIDLYRRG
                     RAYRKKSPTLWCPKCQTAIAQADLEDKSEETFFNYIYFQLENGEKIIIATTRPELLAS
                     CIAVFVNPNDKRYKKLINKNVIVPIFGQKVKIYSDRRVDPLKGTGIVMCCTFGDTTDI
                     DWYIDYQLPVQVSINKNGTMNEFAGSYKGLGIEDAREKIISDFEKKGLLFKKEKIEHI
                     VNTHERCGTKIEILDTTQWFIKYLDFKNDFLSMGRKIKWHPSYMRKRYENWVKGLRWD
                     WCISRQRYFGIPFPVWYCQKCGKVKLAQIKDLPVDPLHTKPKTKCTCGSNKFIPESDV
                     LDTWATSSLSPQIALQLIKNKNLEKKLFPMSLRPQAHDIINFWLFYTIARSKIHFNKI
                     PWKEIMISGFVLDSKGEKMSKSKGNVVAPQEVTEKYGADVLRHWAAKAGPGEDIRWSE
                     EELRSSKRTVIKLWNASRFCFLNLKNFKNKKAKILADEDKWILGAQEEALKNYFQSFD
                     NYEFKKARETVDNFFWHDFCDNYLEIIKPRLYQENFSNTSKESAQKTLYLALLSILKM
                     YSPFMPFITEKLFQMYFRGKEKEKSIHLSLLPKPQKKYLNKKEIKNVETIIEIISAIR
                     KYKSERGLSLKQEIDRLYIDTKNKNIKKYFDLLKSVMNIKEIEFCEIKKEGLEAGKNA
                     KIKILN"
     gene            complement(21473..23059)
                     /locus_tag="P5570_00200"
     CDS             complement(21473..23059)
                     /locus_tag="P5570_00200"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR39179.1"
                     /translation="MNLLAYSGFINAFLVFLIGVFVYFKGRKHQENRVFCFLCLSIVW
                     WQTSYGFWQLATNVEESLFFLKLLMIGSVFSAVFLYHLTLVLLNLVEKNKKILIGAYL
                     ITFSLLSLIFSPLFILETKIFYFYPYWPIAGIGATLNMAFGFVWAIFSIFLLAKNYFR
                     LTGERKYQIGYFLLALFIAAVGGTFNLPQWYGIELYPYGNFIIFLFPLIIAFAILRYH
                     LFDIRIILTELLVGIMGILLLVLPLIVPTNELKILATVLFLLFCIFGYLLIKTTHEQI
                     QKREELERAYNELKELDETKTMFLSIASHQLRTPLSAMKGYLSMVLSGDYGVKIEGQV
                     KEFLEKVYQSNERLINLVNDLLDITRLQMGKLQLNSQPVQIEEIVQSVVDEFSEQARQ
                     KNLSFVFKKPSVPLPKINVDPQKIRQVILNLIDNAIKYTKKGGITVKVQHNSSTNSEN
                     GTILITVKDTGIGLTTKDINNLFQLFERSDSTVKTHAVGVGIGLYAAKEIVKAHRGKI
                     WAESSGRDKGSTFYVELPVK"
     gene            complement(23066..23872)
                     /locus_tag="P5570_00205"
     CDS             complement(23066..23872)
                     /locus_tag="P5570_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR39180.1"
                     /translation="MTKENNQKNKKENLDKIQEEEQKFWKEYADLMSIKKYRDAYIYS
                     PLYQDELKKIKEFLNFNNKEKEKWLDLGCGSLPVTELILEESRGNIELWGGDINLEPA
                     KKRLEELSNSPIKLEYIDLTRKLPFPDNFFDGIVSSKVLPYISNFEGAEGKEAIKKVF
                     REIYRILKPKGVLIWTYNTKDSNNLHGIIVSIGYLLNPYEWIKRKVFLPFYAIKMMGF
                     FKGTIEKLKKGIYHSFSKEEYEDLLTSVGFRNLEWKSGFGDMVLINKAEK"
BASE COUNT         7590 a         4490 c         3308 g         8635 t
ORIGIN      
        1 aaaatcgtgc aggccttggc tgaacttatt cctttaattt ttgtcaagtc ttgataattc
       61 attttaaaaa gccgcttctt ggaatatttt cttaaaactt cttttgccaa gtccaaagcg
      121 ctttttcctt ctctaccagt tcccaataag attgctaaaa gttcttcgtc ttttaaattt
      181 tgaggacctt ttgaaattag tttttctcgc gggcgatctt ctttcggcag gtctttaatt
      241 cttaatttat tttgtggcat aggttaacta aaaaccttag taagataaca atttgataaa
      301 tgattcaaat atataactct ctttttgtgt ttttatctat acagaattat cacctcctat
      361 tttttgaatt tgcaatttgg aagtttgttt tggattgggg atttttacta ttatacctta
      421 aaaaggtgtt tattaccaag aaaataaata ttttttaaac atcaaaaaaa cgctctttaa
      481 aaagggcgtt ttttggtgtt tattgttttt ggcgtaattt ttttacgact attatttttt
      541 tatttcttct atctccagca accgattata ttttgctacc cgttcgcctc tggctggcgc
      601 gccagatttt aatccccaag catttgaagc tattgctaaa tcagcaataa aggtatcatt
      661 ggtttctccg cttcgatgag aaataatcac ttttatatca tttttttgag caaaatttat
      721 tacttctatc gtttcagaca atgtcccaat ttgatttggt tttacaataa ttcctgaaat
      781 aagatctttt tcttttgcct cttttaatct ttcaatatta gttacagtta aatcatcgcc
      841 caccactaaa gtttgattac taatttgttt ttttaattga gtaaaactat tagggtcatt
      901 ttcggaaaaa ggatcttcaa gataaattat ttcaaacctg tttgcccagc gtaaatataa
      961 atctattaat tgttctggag aaaaatgatc ttcaccaata ttatattttt taccatcaaa
     1021 aagttgtgaa gctgcgaggt ctaaacctat ttttattttg tttcttacta gtttttcgct
     1081 aacagtttgg gaaatggctt ctattggaag ttgaacatta gaggtttgag gaacaaagcc
     1141 cccttcgtca cctatattcc ctttaaagaa ttttcccatt tcttgataaa ttcttgcacc
     1201 taagaaaaca ccatctttta aactatctcc ttttactata attaaatatt cttgaatatc
     1261 agggccacca aaggcgtgaa ttccaccatt tacaatattc attagcaaaa acggaaaatc
     1321 agtatctcct ggacgataac cttttaaaaa aaactgagac aaaaattcga aaagtggttt
     1381 ttgttcaatt tttgcttgca attttgctat tgccaatgat acgcctaaaa tagcatttac
     1441 tcccaaatta gatttgtccc tggttccatc tagctcaatc attgttttgt ctgctagttg
     1501 aaaatttact tcttttccta ttaataaagg cgctattttt tcttcaacat tttttattgc
     1561 tttccaagcg ggcaaagaaa gcgcttcata ttttcccacg cttttacctt ctggcacagc
     1621 agctgttgct gtaaaattat cactagcaat ctcaatcttt actgtggtgt tgcttcttga
     1681 atttaaaatt tctatggctt taattctttt tattttttcc ataaatttga gttttaaaat
     1741 tttaataatt agattaattt gggaaattta agtcttcttc ttcggaatta ttattttctt
     1801 tactgtcttc ttttaaataa acatcccttg gttttgcacc ttgacttggt ccaattatcc
     1861 cattctcttc aagcatatca agcaatcgag cagccctagc atagccaatc tttaaacgac
     1921 gctgaagaag cgaagccgat gctttacctg cttccattac tactcgtttt gcttcctcat
     1981 agagttcatc atctccccct ggacctcctt gaaaaagatc agattcaact gtttcattgt
     2041 ttagtgtttc ttctaaactc tcagttaatt tttgggtttt ttcttcttgc tggtgtttta
     2101 ttacaaaatc cactactctt cttatctcct cagaagaaac aaaggcactt tgcaatcttt
     2161 ttggctttgc cgcttcgccc gacaaataca acatatctcc ttgacccaac aatctttctg
     2221 cgcccgccat atctaaaatg gtacgagaat caatctgcga tgccacctta aaagcaaccc
     2281 tagaagtaat atttgctttt ataagacctg ttagcacttc aactgatggc cgttgagtag
     2341 caagcactaa atgaattcct actgcccgtg acatttgagc caaccttaca attactgctt
     2401 caaattcttt tccttttgat gacattatat cagccaattc atcaataatc aatacaatat
     2461 agggaagttt ttcaaattct tcatttcctt ttgcaatttg aatttcaacg aattcattat
     2521 aacttttaat atctcttttt ttagcgtttt gtaaaatact aaatcgatcc tccatttctt
     2581 ttgtcaacca ctttaaaaga ggaataattt tttggtgttg agtaatagga tcgcataaaa
     2641 gatgaggcaa agaggaataa gggctgaatt caactctttt tggatcaacc aaagcaagac
     2701 gcaaatcttg cggaccattc ctccaaagca aagataaaat taaagagttt aaaaatacag
     2761 ttttaccgct gcctgttgca ccagctacta acaagtgagg cattgaaccg atatcaccaa
     2821 aatatggctt gcccattaca tctttcccca atgcaattcc taggggcggc aaaaaaagaa
     2881 attcgggcga agataaaaga tttgccaatc taacctgagc tcttttttga ttaggaacct
     2941 caattcctac taacgatcta ccgggaatgg gtgcttcaat tcttattgga tgagctgcca
     3001 aagccaaagc taaatcattt tgcaaagaca aaattcttga aagttttatt ccctcggctg
     3061 gttttaaggt atattgagtt accgaaggac caatactaac ctcacccatt tcaacatcta
     3121 ttccaaaatt atgaaatgtt cgtttgatga tttgagcatt acttctaata tttccagcga
     3181 ttgcattatt ctcctctcta tcaagcaacg ccaagggaag ggttttataa tcaacctttt
     3241 ttactttttt gataatcggc tcttctgtct tttcaatttt ttctttggag atcttttgag
     3301 gaagaatctc tttaactttt atcttggctt ttccttcttg aagttttttt aattcttcaa
     3361 aagaaagatc tttttcttcc tttttttgtt tagaagaaaa aatttttact tttttaaaaa
     3421 cagaaatatt aaacaaaaga gaaatagaaa ttaacagcag agcaataaaa aaagcaaagg
     3481 aaccagggac gccaaaatac ttttgaggca aagaaataag tgtttttcct aaaaacccgc
     3541 ctgcgtcttc tgaacacaat tccagtaaag aaaaaatggc gataaaaaat aatataccgc
     3601 cactaatagt agaaggataa ggagagggac gttctgaaag aaaaaatatt atcccaatag
     3661 caaaaaatat gatagcaatc agccatgcgc cgttaccgaa aaaaagataa aggacttttg
     3721 aaagactgtt accagcttcg ccccctatcc ccaataaaga gaaaacaaaa aatattccca
     3781 caaaagcaaa aataacaccc caaatacttt tttttgcttc tggagacaga ttaaaaaaac
     3841 gagattgaga gttctttttc cgcaactgcg gttgattttt atttttcttt tttttcttca
     3901 tgaagcgctt tgaaccctgt tccagcaggt attaatttcc ctattataac attttctttc
     3961 aatcctctca atttatcaac ttttccttct actgctgcct gaattaatac tcttgaagtt
     4021 tcttgaaagg aagcagcaga aagaaaacta tcagtagtaa gcgctatttg ggttagtcct
     4081 aataatataa tcttgccttt tgcttcctct tttcctttcc ttcgtaaccg atcattctct
     4141 tcaaagaaag cggctttcga aacaatctct ccacgggaaa aatttgaatc ccctgctttt
     4201 gtaattttta acctagagaa catttgataa attattgtct ctatatgctt gtcatgaata
     4261 gctacaccct caccagtata tattttttga acttctttta aaatatatcg ttgggtttca
     4321 tcaatacccg ctaccttgaa aagaccttta agatttattc tgcccttaaa tataggcatt
     4381 cctggaaaga catcatcacc ttttttaacc aacagttcag tttttactgg cacagaatat
     4441 tctttttgat ctcccttttc agttttaaca ataattgaaa atccatcttt tttctcttga
     4501 atcttttcta tttttccttt ctcttcaaaa attactgttt ctccctttgg cggtcttgtt
     4561 tcaaaaagtt cttctacccg cggtaacccc aaagtgatat cgccaccacc tgccacacca
     4621 ccagtatgaa aagttctcat tgtaagctga gtaccaggtt ctccaatggc ttgcgcggct
     4681 attacaccta tagtttctcc taaatttacc aattgatgcg tggtaagatc tgatccatag
     4741 cacatttgac aaattccttc tttgctttta cagctcaatg gtgaacgaac ccttatttct
     4801 tgaatatctt ttgatttttc aataaaatcc gctgcttctt tactaataag atctcccttt
     4861 ttaagtatta ttttttcacc gtctttaatg tcttgtaaaa taattctccc taatattttc
     4921 tgggacaatt cttgaccaat ttcttctgct tgttttttat aaattacaat tcctttttta
     4981 tcaccacaat ctttttctct tatgattaat tcgtgtgatg catcaactaa acgtctggta
     5041 agatatccag cactcgaagt tcttaaagca gtatcagctg ttccttttct tgctccgtga
     5101 gtagaaataa aatattcaag cgttgttaat ccttctttgt aactgttctt aacaggcaac
     5161 tcaataattt caccagaagg actggcgact aatcccttca ttccagccat ttgagatggt
     5221 tgagaccaag aaccccttga tccagaatct ataatagaaa atactgggcc aaactgcgga
     5281 agggtagatg gtactaattt tgaaatttta tcttttacac cctgccaaac ctcaacaatt
     5341 tttacttttc gttcggtttc tgacaaaagc cccatttcat attgtttatt aatctcttct
     5401 tcttttaacg ctgcttcttt taagagatca ggtttctctt tgggcacaat aagatcgtcc
     5461 attccccagg tgctacaaga gatagttgca tactcaaatc ctaaattctt taaattatct
     5521 aaaatagaaa tgatagttct ctgttcatag tgacctacca gctcagaaat aagcttttga
     5581 atagtttttg aattgtaaac cttattttga taagaaaaat ctttaggcag ggcttcatta
     5641 aaaataattc tgcccactga agtttcaata agtttattat tttctttttt aatccttact
     5701 tttattacag cttgtaagtc aatttgatct agattataag caatctttgc ttcttcttca
     5761 ctagaaaatg ctctaccttc tcctctggcg ccaggtcgaa cagtagttaa gaaatagcaa
     5821 cccaacacca tttcttgtcg tggaatagca atgggctgac ctgttgctgg ttttaaaaca
     5881 ccctttgaag aaatcatcaa atttcgtgct tctgcttgcg ctttttttga aaggggtaga
     5941 tgaacagcca tttggtcacc atcaaaatca gcattaaaag ccggacaaat aagaggatga
     6001 acgtgcaaag cttgatcttc aatcaatact ggtctaaagg cctgaataga aagacggtga
     6061 agggtaggcg ctctatttaa aagaacatat tttcctctta tcacctcttc caatatttcc
     6121 catacctcac ttctgccctc tgaaatcaaa ataccagctg ctctaatatt atgagcaagt
     6181 cctcgttcaa taattttgtt aataataaat ggtttaaaga gctccaaagc cattttcttt
     6241 ggcaatccta cttcttcaat atctaattct ggaccaacta caatcactga cctaccagaa
     6301 taatccactc ttttacctaa taaattttga cgaaatcttc cttgcttccc cctaagcata
     6361 tcagctaaag attttaattg tcttctttgt ccagtagatg cttggaccat ttgagtttta
     6421 cgcgatgagt tatcgattaa agcgtctact gcttcctgaa gcattctttt ttcatttcta
     6481 acaatcacct cgggcgcacc aatctctttt aaatatttta aacgattatt tctattgatt
     6541 acccgacgat aaagatcgtt taaatcagaa gaagcatatc tgccaccatc taattgaacc
     6601 attggccgta aatcaggcgg taaaaccggt aaatatgtaa aacacatcca ttctggttta
     6661 gcaccagaac gaattaatga gcgaaccaat cttaaacgtt gagtagcata ttttttagta
     6721 ctagtttttt ctaattcttc ttttgattct gcttctaatt ttttgagatc aatagattca
     6781 agtaattttt ttattgcttc agcgccagtt gatgcccaaa aacactctcc atattttaaa
     6841 gaaagccgac gataatcatt ttctgaaata atagtacctt ttttaatact tgcaatttct
     6901 tctttggctt tttgaagttc ctcttttaat gcttgttttt cttctttact tttacctttc
     6961 ttttttcttt caaattcttc tttgatctgt aaaataattt tcttttttga ctcttcatcc
     7021 acttccacaa taatataaga cataaagtaa accactcttt caacctgctg cattgatttt
     7081 cccaataaaa gaccaattct tgacggcacg ccccttaaaa accaaatatg ggctactggt
     7141 acagctagct taatatgtcc caatctttct cgccgaacag atgattttgt tacctcaaca
     7201 ccacacttat cacatactac tcctttgtaa cgaatctttc gatattttcc acaataacac
     7261 tcataatctc tcgttggacc aaaaattttt tcacaaaaca gcccatcttt ttctggacgc
     7321 tgagttcgat aattaatagt ttcaggtttt gttacttccc catgagacca cgataaaatt
     7381 tccttgggcg aagccaatct tatttttatt gcatcaaaat cttctacaat tgacataaaa
     7441 ttagaataaa gaaggtcaaa aatcgttttt atattatttt ctgccttctc tttttattaa
     7501 ttccacattt aaacctaatg attttaattc tgcaagtaaa acattaaagg tagatggtaa
     7561 atgagaagtt tttatttcct caccttttac aattgcttca taagtggttg atcttccaat
     7621 aacatcatct gattttatcg ttaacatttc ttgaagagta taagcagcac cataaccttc
     7681 tagcgcccat acttccattt ctccaaatct ctgtcctcca aactgagctt ttcctcctaa
     7741 gggttgttga gtaattaaag agtaagggcc aattgaacgc atatgcattt tatcttccac
     7801 catatgaatc aatttcatta catacatata tccaacagta attttttctc tgaatggttc
     7861 tcctgttttt ccatcaaata gtttcacctt tccattctcg ggcaaaccgg cttttttaag
     7921 ttctctttta atgtcttgtt ctgttgctcc atcaaatgcc ggagatactg cttgatatcc
     7981 caattttttt gcagcaaaag ctaaatgggt ttccaatatt tggccgatat tcattctgga
     8041 agcaactgaa agagggttta aaataatatc aataggtgtt ccatcttcca aaaatggcat
     8101 ttcttcttct ggaagaattt tagcaattac tcctttatta ccatgccgtc ctgccagttt
     8161 gtctcctact gaaatttttc ttaaataagc aacctcaacc tgaattcttt ttattacgcc
     8221 tggctccagc ttataccctt tattacgctc aaaaaccttt actctcaata ccttgcctct
     8281 ttttccatgg ggcagggtta aagaagtatc ttttacttct tttgcttctt ctccaaaaat
     8341 tttctttaaa agcatttctt cagaagataa atcttcttca cctttaggag aaacctttcc
     8401 tactaaaata tctcctgctt ctacttcggc accaattcta ataattccat ctttgtctaa
     8461 attacttaat tttgcttctg aaacattagg aatatctgct gtagttgttt ctggtcccaa
     8521 ctttgtttcc ctaacatcgc acataaagtc ttcaatatgc aaggaagtaa aaacatcttg
     8581 tttggcaact cgttcagaaa gaataattgc atcttcataa ttagcgcccc gccaaggtaa
     8641 aaaagcacaa agaagatttt tacccaaaga caattcacca ttagcaacag caacgccttc
     8701 tgctaataaa tcaccctttt tcaccttatt gccaatttct acaacaggtc tttgtgaaat
     8761 acttgtatat tggtttgatt ttataaagtt taataatgga tactctttta tttcacctct
     8821 ttttgtttca attgcaatgt gagatgcatc aaccttaaca acctttccgt cttcttcagc
     8881 gaatattaat tgaccagaat tttcagctac ttttttttct gctcctgttg ccactagtgg
     8941 tggttctggt cgtaaacaag gaacagattg cttttgcata ttacatccca ttaaagccct
     9001 gtttgcatcg ttatgctcca aaaatggtat taaagaagca gccattgata aaatttgctg
     9061 aggagagaca tcaatatagt caattttatc ggtctcgata acaccaggtt gaccattaat
     9121 tctagcttct attttctgag caataatttt gccatttgca ttgcggggtg tagtagccgg
     9181 tgcaatatta tatttttcct cctcataacc gttcaaataa tccaccttat tagtaacttt
     9241 tcctttctca actttaaaat aaggggtctc taaaaatccc aatcggttga ttcggctgaa
     9301 agcggctaaa tgagtaataa taccaacatt ttgaccttcg ggtgtttgaa taggacaaat
     9361 tcttccatag tgactgggct gaacatctct tacctcaaat ccagctcgtt ccctggtaag
     9421 accaccagga cccaaagccg aaagccggcg tttatgctct acctcggcta aaggattttc
     9481 attatccata aattgagaca actgagaaag agtgaaaaat tcttttacct gtgaagcaaa
     9541 aggatgagga ttaataatat tttttggttc tgcttcagaa gcatcaatag tagacatttt
     9601 gtcttttatt attctttcca tgcgaagcat acccactctt aatcttgatt caagcaactc
     9661 agcaagggtt cgaatacgcc tattccccaa atgatcaata ttatcgggtt gagcatgagg
     9721 atttttattt aattcaataa tttcttttaa tacaacaata atatcttgag ggtttaaaag
     9781 gcgatctttg gcagtaatat tcttaacctc tggtaacctc attcgttgtt gaattctcca
     9841 tcgccctact tcagataaat catatctttg agggttaaaa aataatcctt caaaaaatcc
     9901 tcttgcattt tctactgtta ctaaatcccc tggccttaaa tggttataaa tttccattat
     9961 cgcctcatct tgatttttcg ctttgtcttt ttgtaaagtt tcttcaatgt aattaatttc
    10021 acattctttt aaattttctt caaacaattc tttaatctct gcatttgata aaggactaaa
    10081 agctcgtaaa agagtggtag ctaataccct tctttttcta tcaattttta cccacaatac
    10141 accattttta tctgtttcaa attccaacca tgctcccctg ttaggaatta attttgctcc
    10201 aaatagtttt tttccataaa aataattagc cgtaaaaaaa acgccgggag atcgaattaa
    10261 ttgtgaaacg atgactctct cgacgccatt taaaataaaa ctgccacttt tggtaattaa
    10321 aggaaaatta caaagataaa cttctccttt tctttcttcc ccctgacttc ttattttaag
    10381 acgcgcctga acaaaaagcg atgcttcata agaagcatta ttttcctttg ctttgtcagc
    10441 gtccattttt ggctcctgca atttaaaacc atcaaagtaa agttcaaatt ctttacctga
    10501 ataatcctta ataggagaaa agtcttttag aagattattt aatctctttt ctaaaaactc
    10561 ttgataagag tcaatttgaa atcttatcaa gtttggataa ggaaattgaa aagaatattt
    10621 ggaaaagata tttttctcca tagaaaaaag aaaataacaa aaaataaacc caaatcgaaa
    10681 tgggttaaaa tccatttaaa ggttaaataa aaaagaaaag ataatcaaga taaaattatt
    10741 tttagtttat ttatatttta tatttttaaa atagaattgt caaggggttg aaaaaatttt
    10801 ccctattttt aagtatacca aatttttaaa agaaaagcaa gggttaatag aaaagcccat
    10861 caaattaaat aatgatatga tgggcaggca ctttgtttaa aaaagattaa aaatttttta
    10921 caaattcttt catctttctt ttataatttt tcttttcctc ttcttgtgtt ttttctaata
    10981 tttttttagc ctctttttga aagtcgtttt tcaaggtggc caaggaaaaa attgtcataa
    11041 aataatctag cttactcacc tttttctctt gggcaaatat ttcaatattt ttgaagaaaa
    11101 accattttac atttttttta cttggatttt cttcaacaag aattggataa aaaagaacca
    11161 atcctttctg atctactatt ggataaactg gaaaaaaatc atccgttggt attctcatgc
    11221 ccgcttgact ttcaacaatt ttgctaagct ctttattaac ccaatctctc tctgcttcaa
    11281 aaaattcagg cagtactggg ttcatagcgc ttcctagtcc acacaaacag taatgatcgt
    11341 tttccctggc tacacgggga atctttactt cccctccggg aagacttaca aagggcctaa
    11401 ataattcttc acaaaaaagt actttcccat ttttctttac aaacactgca tacgccaata
    11461 gtttcgtttc catctttaat ccctcctttt ttcaaagtgc tggaaagaat tcctccaatt
    11521 ctctccgata ctatcataac aaaaaattat tttttgtcaa taaagcgttg gtttggttgt
    11581 tggcgataaa gaaatggagc ataaaagcgt cagagtacga gagagaggaa aaggagatat
    11641 tggcgaaata aaattagaaa aatttttaga aaaaattaaa aaatagatat ttaaagaaat
    11701 aaaaaaagca gttttaagac gtgcttaggt ctgaatctcg tttttgagaa tttcaattat
    11761 aattggaatc gctgactttg gtaccatttc tgcctttttc ggaattagtc ctgaatgact
    11821 ggtatgaagg ataaaaaatt ttccttcttc tttcattggg atagctgagt tgatagggac
    11881 tctctcgtct ctatcgctcc cgataaaata ataaccttca ggatgccaat tccagtttag
    11941 taatcttaaa aaattactct ctggttgaat ctcccttaca caagggaggc tgacaagttt
    12001 accaactaac ttgtcttcta caaaattaat ctttacgccc ctgttgggag caccaactag
    12061 aattacctcc ttggctttta atcctatttt ttctactaaa aatcttgtta taattccacc
    12121 catactatag ccgattaaaa tcggctcatc attgttaata aaaagggttt ccctttgtac
    12181 gttccaggcg tactccggga ttgtttttgt tcggagatac tttcttagct ttccataccg
    12241 ttcaaagtac tcgggaacca gtacttcttc ttccggaaaa ttttttttaa tctcttcttc
    12301 aagcgtttta agacgctttg atcccgtaga ggagaatcct cccacaataa tgatcatttt
    12361 ttacacctcc catctttacg acgggaattt aactgctaat ttagtagtaa tcaaatttcc
    12421 atcgtaaaga tgctttcttt ttctattttt aaagaactat ttacctcctt tttatattat
    12481 aacatattct attatttttt gtcaataaaa cgctggttta aatgctatca tattacaaac
    12541 ttagaaacaa agcatatcta tctgaacttg gattagggca ttagtgaaat ggggatggag
    12601 ggagtcgaac cctcacggac ttgaagtcca atggtttttg agaccatttt gtctaccagt
    12661 tccaacacat ccccttaata aatttacgtg tttagtttag cagaaaatac ttcaaatttc
    12721 aacttgaaaa taaaaagttt atttgctatt attctttaat agaaaaccat ttggcatttt
    12781 ataaaattat tttatgactc gtgttatttc aatcattaat gagaaagggg gctctggaaa
    12841 aaccaccact gctgtctctt tgggtgcttt tcttacaaaa aatggcaaaa aagtgctatt
    12901 gtgtgattta gatcctcagg gcaatgccac tacgtcatta ggtcttgatc taaagcattt
    12961 atctttaaat ttttatcatg ctctttccca gcaagtgcca ttaaaagata ttattaaacc
    13021 tacttctctt tttaattttg acattgctcc ggcaagcgct gatctggcag gaagcaatat
    13081 tgaactacta gacaaagaaa acagagaaaa tattttaagc aatattttat cacccctaaa
    13141 agaagattat gattttattt tgcttgatcc tccgccctca ctgggtattt taaccatcaa
    13201 tgcgttgata gcggccgatg aagtaatcgt tcctattcag tgcgaatttt ttgctcttaa
    13261 aagtttagat gaattatttg aaatttttaa ccttttaaaa aggaatctga ataaagaatt
    13321 ctcccaaata tttgggcttt taacaatgta cgacaaaaga aatatccttt ctagagaaat
    13381 tgccaaagaa gcagaaaaaa aatttccagg aaaaatgtta gaaacagtta ttcccagaag
    13441 tattagattg gcagaagcac caaaatatgg taaaacaatc ttacaatatg ctcccgaatc
    13501 caaaggcgca agggcatatg aaagtttagc agaagaaata ataaatttta aaatttaaaa
    13561 ctttattgtt ttaaaattga aaattaaaaa ttttattgtt atgtctggtc ttgaatcttt
    13621 aattcccaaa aaaataaata aatcgacttc ccaaaaaaaa gaaagtgttt tttggattga
    13681 aattgataaa atcaaaacta atccatttca gcccagaaaa gaatttaatc aaaaagaact
    13741 aaaagagtta gcagattcta ttgaaaaata tggaatactt caacccctta ttgtaagaaa
    13801 aattgaaaaa gatgtaccca atggacaaaa ggttgaatat caattaattg ctggtgaacg
    13861 cagattgcga gcttcaaaaa tgataggatt aaaagaagtg ccagtaatag taaaagagat
    13921 atcaaaagaa aatgaactgc ctatttccct agtagaaaac attcaaagag aagatttaaa
    13981 tcccttagaa aaagcactgg ctttcaaaaa attaattgag gaatttcatt taactcaaaa
    14041 agaagtagca aaaatggttg gaaagagtag agaatcaata gcaaatacct tgcgactttt
    14101 aatattgcca gaagaaattc aaaaaggaat tcaaagagga gaaatttctg aaggacatgc
    14161 ccgtgcccta ctttcaatac caaaagaaaa acaaatagat gtttataagg aaacaatcaa
    14221 aaagaaacta ccagtgagag aattggaaca aaaggcagaa tcaaaaaaag agaaaaaaga
    14281 aattttaaat cttaaaaaac tgcaagacga aatctttcaa gtttttaaaa taaataattt
    14341 caaaataaga gagaaaaata ataaaataga attaacgctt aactttaatt ctgaaaaaga
    14401 attaaggaat tttttgaaag agattaaacc ttcttaatcg ctaaccaaaa gacctttccg
    14461 tcttcattaa gttgccgctc tattaggtat ggcggttttt ttgttgtggt aatttctttg
    14521 gcaagtgtaa aattctttat taattcttca ttctttttga aaatcacagc aaaatctttt
    14581 ggaaaatcat aaataatctg aattttatct tttggtttta atttaacact ttttctcatt
    14641 tcttgaattt gatgcaaaat ttctcttaaa attccttcat tttttaattc aggtgtaatc
    14701 ttagtatcta actctacttt tccattgatg ttagaatcaa aaattacttc tttaacattt
    14761 aactcatctt taatcaaatt taataaccct tcattatttt tgatttttga tgggcgaaga
    14821 gaactttctt ctcttcttcc gttgaagctt cggccgagac gcgaaaagaa taaaattctt
    14881 tgagcatctc gttttgttgt tgcaaaactc gatatttggt ttttgatttt gagcgaagcg
    14941 attggttgtc ttacctttat acctgatttt tttctttcag ctaatcccaa agctgcaatc
    15001 tcttttactt cattcatttt ttcttctaaa tttttattga gaaattcttt ttttatctga
    15061 ggaaagttct ctaaatgaac tgattttttt ctttcaaaat taaatccttt aactttttgg
    15121 taaatttctt cgctcaaaaa tggtgtaaat ggtgctaaca gtaatgaaag gtttaacaaa
    15181 acgtaattaa aggtaaaaat agtaccccta tcaccttttt tgagtcgttc ccttgatctc
    15241 cttaaatacc aatttgacag atcatcaacc acaaaatttt caatcaatcg tgctgccttt
    15301 actacatcat aatttgataa aagttgatca acatcttttt taagattttc cgtctttgat
    15361 agaatccatc tatctaaaat gttttttatc tctgttgaag acgcttttga tttttttgta
    15421 taaagcaaaa gaaaattaag caggttttca acagttaaaa taaaacgatt ataggttttt
    15481 cttagatctt tttcatcaaa tctttttcct tcccaaggtt gattaacagt aaaaaaataa
    15541 aatctaatag catcagcacc atatttatta atcatttctt ccggaaaaac tgcatttcct
    15601 tttgactttg acattttttt acctttttta tctagaacca atcctagaac taaaacattt
    15661 ttatatggag tggaaaaacc caaaagcgag gaaaccgcta ataacgtgta aaaccaccct
    15721 cgtgtttgat ctattccttc acaaataaaa tttgcaggaa ataattctgg tggctttgat
    15781 tttttatttt tgggctgact aaacggccaa taattttgag caaagggcat tgcaccagaa
    15841 tcaaaccaaa catcacccac ttcctttact cgttgcatct tttttctttt acaattggga
    15901 caataaaatt ctatttcatc aacaaaagga cggtgaaaat ctaattctcc ttttttgttg
    15961 taaggaaatt ttttcatttc gatctccctt agctcacctt tgctcatcat tattttgtct
    16021 cttgatttta atatttcttc attattcaat cctttcatta cgctttccaa tagccacaag
    16081 ggatcgccat gactgacaat taaaatattt ttattttggt acttcttttc aacattattt
    16141 ataaacttta acattctcaa tttacaatca ttccagcttt cacccttggg caaacgttgt
    16201 tcaaatctta aaatgctaaa aggaaaatca tttcggtatt tttcaaccgt agatccatca
    16261 tattctccaa aattcaattc tcgcaatttt gaagaaaaaa ttacttttgt tcctaattcc
    16321 cttgaaacaa tttctgatgt ttctttacac ctttgaatat cagaagaaaa aataatatct
    16381 aatttttctt tgcccaataa tttttttaat tttaaaacag attttttaac ttctaatttt
    16441 cctatgtttg ttaaatgaaa tttattttgc aaagaagaac tgagaatacc ttttacattc
    16501 tgttgcgcca acccatgcct taaaataaaa taacggtttg ttgaaaattt ttgttttcta
    16561 atatcctctc gagaaccaat tacctcaata ttattacaat tattacactt ccaaataggc
    16621 aatggcgttc cccagtatct ctctcttgaa aaattccaat cctgaatttc ttctaaccac
    16681 tttccaaatc ttccttcttt tatgaaagga ggaacccaat ttactttttg attatttttg
    16741 atcaattttt ctttaatttt ttccgtagca aaaaaccaag aggaatgaag ataatacaag
    16801 agtggatttt tacatctcca acagaaagga tattcatgct catataattc ttcctttaaa
    16861 agtaaattac ggttttttaa atctccgatt attaaaggat cagcgtcttt aacaaacaat
    16921 ttatcccatt tatatcctgg cgttttcatt ttgccctgtt catcaacggg tttaaaaatt
    16981 gaaaatttta aatttgaatt ttgctgccgc caaacagcca tatcatcctc gccaaaagcc
    17041 ggagcaatat gcacaatgcc agttccctca tcagtggaaa caaaatcacc agcaacaacg
    17101 taatagattt tggattgatt tggagtttgg aagtcgaggt ttggagtttg atataatggt
    17161 tcatattcca agccaacaag atcctttccc ttaaattctt taataatatg aaatttactt
    17221 tcatctttaa aaagatattt tattctttct ttcgctaata taaattgctc cccttcgttt
    17281 tccattagca cataatcaat cttagaattt actgccaatg ccatattgcc aggcaatgtc
    17341 cagggtgtag ttgtccaggc caaaaaataa atgttagatt ttgaatggtt attgaaaatt
    17401 ttatttttat ttcttgcctc actttgattt tgttgagtgt tgaatttgtt gccttttgaa
    17461 tctttatttt tgagttttaa ttttatgtat attgccggat cttttacttt ttgatatcct
    17521 agagaaactt catgagaaga taaagaggtt tgacatctgg gacaccaagg aacaattttt
    17581 tcatcttcat acaaaagtcc ctttttataa atttctttta ttgtgtacca taaactttca
    17641 atataaagtg gatcataagt aatataggcg ttatcaaaat caagccaaaa accaatttcc
    17701 tcggtcattt tttcccactc tgttttatag cgccagacaa ggtttttgca gttttcaaca
    17761 aatttatcaa tacccaattt ttcaatatct tgtttcgttt taatctgaag ctgcttttca
    17821 acttctaatt ccactggcaa gccatgggta tcccagccag cccttctctc aacataaaaa
    17881 cccttcatcg ctttataccg acaaataatg tctttataac ttcgcgacaa aacatgatga
    17941 agaccaggtc ttccgttggc accaggagga ccctcgtaaa aaataaattt ggggttcttt
    18001 ttgctttttt gaagtccttt ttcaaaaatt ttttcttttt tccaaaattt aagaatttct
    18061 ttttcttttt ctgatgtttt catatttttt gtcaaataaa gtatttgcgc tttttattct
    18121 gcctcaatga gtttaaaact gggtagaatt aatgtcttta catcgtaaat agggccttca
    18181 taaaatctcc ccggtcctgg atcctcagga aatcccttaa agcccatatc gtcttgacac
    18241 atagaaatag taaaaacata tttatcattc tcagttatat aagctggcgt tggaccttcc
    18301 tcttgttgta gtgccttcca ttgatcttta cttaaaatag ttattttaaa aattgatcca
    18361 taatattcat tatcagaaac caacgattcc tccctttcta attgaaaact tatagtttca
    18421 attccccaaa cactatcctc ttcaacaact ttataacctt gccatctctc tggaaaagtt
    18481 agctcaaatc cgtactcctc atttcgatag atctgccaat tagtagttcc atttttaata
    18541 gtctcttctg gttcagatac tgtcccttct ggttcagata cttcttccgg cgcacttacc
    18601 tcttctgatt cgaccatctc ttctggtgct tttatttctt gttttgttgt tccaaaatac
    18661 tgccaagtaa aaattcctcc accaataata atgatagaaa ctattactat aatcaaaatt
    18721 tgggaaaaag atgcattttt catatttttt atcaaaatta gtttaaaatt tttatttttg
    18781 cgtttttccc tgcctccaat ccttcttttt taatttcaca aaattcaatt tcttttatat
    18841 tcattactga ttttaataaa tcaaaatatt ttttgatatt tttgtttttg gtatcaatgt
    18901 ataatctatc aatttcttgc tttaaagaca accccctttc acttttatat tttctaatcg
    18961 ctgaaataat ttcaataatt gtttcaacat ttttaatttc ttttttattt aaatatttct
    19021 tttggggttt tggtagcaaa gaaagatgaa tgcttttttc tttctctttg cctcgaaagt
    19081 acatttgaaa aagtttttcg gtaataaagg gcataaaggg tgaatacatt ttaagaattg
    19141 aaagtaatgc aagataaagg gttttttgag cactttcttt tgaggtattg ctaaaatttt
    19201 cttgatacag tcgcggcttt ataatttcca aataattgtc acaaaaatcg tgccaaaaaa
    19261 aattatcaac agtctctctt gcttttttaa attcataatt atcaaaagat tgaaaataat
    19321 tttttaaagc ttcttcttga gctcccaaaa tccatttatc ttcgtctgct aaaatttttg
    19381 cctttttgtt tttaaagttt ttcaaattca aaaagcaaaa tcgagaggca ttccaaagct
    19441 taataactgt tcttttgctt gaccttaatt cttcttcgct ccaacgaata tcttctcccg
    19501 gtcccgcttt tgctgcccag tgtcttaaaa catcagctcc gtatttttca gtcacttctt
    19561 gaggtgccac cacattaccc ttactctttg acattttttc cccttttgaa tccaaaacaa
    19621 aaccagaaat cattatttct ttccaaggaa ttttattaaa gtgaattttt gaacgagcaa
    19681 tcgtatagaa aagccaaaaa ttaataatat catgggcttg agggcgcaaa gacattggaa
    19741 ataatttttt ctctaaattt ttatttttta ttaattgaag agcaatttga ggacttaaag
    19801 aacttgttgc ccaagtatca agaacatcac tctctggaat aaatttatta ctaccgcagg
    19861 tacattttgt ttttggcttg gtatgaagtg gatcaaccgg caaatctttt atttgtgcta
    19921 atttaacctt gccacatttt tgacaatacc acactggaaa aggaatacca aaatatcttt
    19981 gacgagaaat acaccaatcc caacgcaatc cctttaccca attttcatag cgctttcgca
    20041 tataacttgg gtgccatttt attttccttc ccattgaaag aaaatcgttt ttaaaatcaa
    20101 gatattttat aaaccattga gtggtatcta aaatttctat ttttgttcca caacgctcat
    20161 gagtatttac aatgtgttca attttttctt ttttaaaaag taatcctttt ttttcaaaat
    20221 cactaataat tttttcacgg gcatcttcaa ttcctaagcc cttataagaa cctgcaaatt
    20281 cattcattgt tccattttta ttaatagaaa cttgaaccgg caattgataa tcaatatacc
    20341 aatcaatgtc agttgtgtcc ccaaaagtac agcacatcac aatacccgtc ccctttagtg
    20401 gatcaactct tctatcacta taaattttaa ccttttggcc aaaaattgga acaataacgt
    20461 ttttgtttat taatttttta tatcttttat catttgggtt tacaaacaca gcaatgcaac
    20521 ttgctagcaa ttctggacga gttgtagcaa taataatttt ttctccattt tctaattgaa
    20581 aataaatata attaaaaaat gtttcttcgc ttttatcttc aagatcagct tgagcaatgg
    20641 cagtttgaca tttagggcac caaagagttg gtgatttttt acgataagcc cgcccccttc
    20701 gataaagatc aataaaaaat cgctgactta atctttgaac attcttatca atggtagaat
    20761 aacaaagatc aaaatcagat gatattccaa ttttcctcca atccttcaca aaatctggcc
    20821 ttatttctcg aagagttttt tgacataaat gggtaaattc ttctctttcg aaatcaaaaa
    20881 ttttaacatt gtttaatttt tcaaccaatt tttcagtagg taatccattg tcatcagtcc
    20941 caaagggata aaaaatattt tctcctttta aacgatgata tcttacaata aaatcttgtt
    21001 gagaataaga aaaagcgtgt cctaaatgca ttttaccgct aaccgttggc ggtggcgtat
    21061 caacagcaaa aaacgaccct tttttcttag gattaaattt ataaattttt tcttttttcc
    21121 aaaaagaata aatttctttt tcaacttttt gaggattata ttgtttttgc atttttatat
    21181 ctataaatgt tgtttatact ttatttaaat tcaaaaattt aacactgtat ccttggtcga
    21241 tgaagtttac ctcttacaga gtttttttca tcttcgcctt cgttcgcttt caacgaactt
    21301 atatgagtag aatattaata atagtcattt ttctgaatcg ggtcgaagaa tttatcttgg
    21361 acaaattgaa ccctttaaca aattcaggaa gtaaataagt attgtatcaa aaattgaatt
    21421 tgctttatat ctctattcta ccaaatttaa tttataaatt aaatattctt ttttacttta
    21481 ctggtaattc aacataaaag gtagaaccct tgtctctgcc ggaagattct gcccagatct
    21541 tgcctctatg ggctttaaca atctctttag ccgcataaag accaatacca acaccaacag
    21601 catgagtttt aacagtagaa tcgcttcttt caaataattg aaaaagatta ttgatatctt
    21661 ttgtggtaag gcctatacca gtatctttga cagtgattaa aatagtacca ttctctgaat
    21721 ttgtagagga attatgttga acttttacag tgattcctcc tttctttgtg tacttaatgg
    21781 cattatcaat aagattaaga attacttgtc tgattttttg aggatctaca ttaatcttag
    21841 gcaagggcac agatggtttt ttaaaaacaa aagaaagatt tttttggcga gcttgttcac
    21901 taaattcatc tactacactc tgaacaatct cttctatctg aacgggttga gaattaagtt
    21961 gtaatttacc catttgaaga cgagtaatat ctaaaagatc attaactaga ttaataagac
    22021 gctcattaga ttgatatacc ttttcaagaa actctttgac ttgaccctct atcttgacac
    22081 cgtaatcacc tgaaagcacc attgaaagat atcctttcat tgcagaaaga ggtgttctca
    22141 gttgatgaga ggcaatagaa agaaacattg tttttgtttc gtctaattct ttcaattcat
    22201 tatatgccct ctcaagctct tccctctttt gaatttgttc gtgagtggtc ttgattaaaa
    22261 gataaccaaa aatacaaaac aataaaaaga gaacagtagc gagaatcttt agctcattgg
    22321 taggcactat caaaggaagg accaaaagca agatacccat tattcccacc aaaagttctg
    22381 ttaagattat cctaatatca aaaaggtgat aacgaagaat ggcaaaagca ataattaaag
    22441 gaaaaagaaa gattataaag ttaccgtagg gatacagttc aataccgtac cattgaggaa
    22501 gattgaaagt gcctcctact gcagcgataa aaagagctaa aagaaaataa ccaatttgat
    22561 atttcctttc cccagttaac cggaaatagt tttttgccag taagaaaatt gagaaaatag
    22621 cccatacaaa accaaatgcc atattaagag tagctcctat tccagctatg ggccaataag
    22681 gatagaagta aaatattttt gtctctaaaa taaaaagagg tgaaaaaatt aaactaagga
    22741 gactaaaagt aattaaatat gctcctatca aaatcttttt atttttctca acgaggttta
    22801 aaagaactaa agtaagatga tatagaaata cggcagaaaa aacgctgcca atcatcaaga
    22861 gtttaagaaa aaataatgat tcttctacgt tagtggctaa ctgccaaaat ccataactag
    22921 tctgccacca aacaattgat aaacataaaa aacaaaagac tcgattttcc tgatgctttc
    22981 tacctttaaa ataaacaaaa actcctatta agaaaactaa aaaagcattt ataaaaccag
    23041 aataagcaag taaattcata taaatttatt tttctgcctt atttattaag accatatcac
    23101 caaaaccact tttccattct aaattacgaa aaccgactga agttaataaa tcttcgtatt
    23161 cctctttgga aaaagagtga tagattcctt tttttaattt ttcaatagtg cccttaaaaa
    23221 accccatcat ttttattgca taaaaaggta aaaatacttt tcgttttatc cattcatagg
    23281 gattcaagag gtaaccaata ctaacaatta ttccatgtaa attattagaa tcctttgtgt
    23341 tataagtcca aattaatact cctttcggtt ttaaaattct atatatctct cggaaaactt
    23401 tttttattgc ttcttttcct tcagcgcctt caaagtttga aatataaggc aataccttac
    23461 ttgatacaat tccatcaaaa aaattatctg gaaatggcaa tttccgagtc aaatcaatat
    23521 attctaattt tattggagaa ttacttaatt cttctaatcg ttttttagct ggttctaaat
    23581 taatatctcc tccccaaagt tcaatatttc ctcttgattc ttccaaaatc aattccgtta
    23641 ctggtaaaga cccacaaccc agatctagcc atttttcctt ttctttatta ttaaaattta
    23701 aaaattcttt aattttttta agttcatcct gataaagcgg actatagata taagcgtctc
    23761 tatatttttt aatcgacatt aaatctgcat attctttcca aaatttctgt tcttcttcct
    23821 gaattttatc taaattttct tttttatttt tttgattatt ttcttttgtc atggttttta
    23881 aaagacagtt ttttctttta aaagaaacct gcctttggtt aatagttgat tagtaaaaat
    23941 ataaataatt cattattgca actaacacaa aagggctcat tttgagccct tttgaaatac
    24001 ctcaacaacc tttgagtttt gga
//
LOCUS       DAOTQD010000003        20814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_2618_length_20814_cov_7.721952, whole genome shotgun sequence.
ACCESSION   DAOTQD010000003 DAOTQD010000000
VERSION     DAOTQD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20814
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_20814_cov_7.721952"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..950
                     /locus_tag="P5570_00210"
     CDS             228..950
                     /locus_tag="P5570_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRR39181.1"
                     /translation="MRSGKYDDKNAFLSIYAGTGGKDAEDWVSILCRMYQRYLQKKGL
                     KTKEIQKISGEGGIKSVTYEVKGKGAYGLLKGENGVHRLVRISPFSAKNLRHTSFALV
                     EVLPEIETEEIKIQDKDLKVETFRSSGAGGQYVNRRESAVRIVHLPTGISVTSQEERL
                     QGENKKRALQILQAKLLQKREEENAEKIENERGELPLADWGRQIRSYVFHPYQMVKDH
                     RTGIKTSNLKKVLDGDLDKFIK"
     gene            1017..1697
                     /gene="ftsE"
                     /locus_tag="P5570_00215"
     CDS             1017..1697
                     /gene="ftsE"
                     /locus_tag="P5570_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRR39182.1"
                     /translation="MIYFNNVTKVFSNNCVALSDISFGVSDGEFVSIAGKSGAGKSTL
                     IRLLLNEERPTQGEIIFEDINVNKMSPSYLPYYRRKIGVVYQDYKLLPQKTVFENVAF
                     ALEVLGKSESEIKRVVPQALDLVGILDKASNFPKELSGGEQQRTALARAIVIQPRVLV
                     ADEPTGNLDMITTKEIVKLFLKINQLGTAVILTTHNREIVNSIQKRLIVLDKGKIIRD
                     EEKGRYYL"
     gene            1797..2714
                     /locus_tag="P5570_00220"
     CDS             1797..2714
                     /locus_tag="P5570_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRR39183.1"
                     /translation="MFNNFFRVFKFGWQEISRNMGISIGTIFIIFIALCLVGGTFLLR
                     GMSENLITTLENKVDVSVYFKDDAKEEDVLLVKKQIEEFPEVKEVEYISKEKALETFK
                     ETHKNNQLLMESLTEIGSNPLPSSLNIKAIQASSYENLTNFLMNGEYKNIIEKINWSE
                     NQAIIERLFSISNAVKTGGFAASLILVLIAVIVTFNTIRLAIYSKREEIETMKLIGAT
                     NWFIRGPFVVQGLITGFFAGLIAFLFFYGVEMGLSSRGLGIFGELGFFNFFEENILLF
                     LIIQIGGGIIISTFASLLAVQRYLKTEKN"
     gene            complement(2765..2959)
                     /gene="rpsR"
                     /locus_tag="P5570_00225"
     CDS             complement(2765..2959)
                     /gene="rpsR"
                     /locus_tag="P5570_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRR39184.1"
                     /translation="MACYFCQRNINEIDWKNEQLLSRYISAGAKIKSRKKTGVCNAHQ
                     RKLKKAIKKAREMAILPYIK"
     gene            complement(3064..3513)
                     /gene="ssb"
                     /locus_tag="P5570_00230"
     CDS             complement(3064..3513)
                     /gene="ssb"
                     /locus_tag="P5570_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRR39185.1"
                     /translation="MNLNKVFILGRVTQDLQAKSLPSGQTVTNFSVATNRVFYDKNRE
                     KQEQTEFHNVVAFGKLAETAVQYLKKGDIVLIEGHLQTRSWEKDGIKRYRTEIITEML
                     QLGPKNQSSAFYQRPAPSVQPPQPEIKEEEIPIIEEEEEIDVKDLPL"
     gene            complement(3584..3988)
                     /locus_tag="P5570_00235"
     CDS             complement(3584..3988)
                     /locus_tag="P5570_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRR39186.1"
                     /translation="MNYELDFLTPNLDTKEKEDLLKTIEEKIKKLNGTIQDQLIKKRL
                     FAYPVKKQRVGFLGVTQFTIEGSKIKELNDFLKMNENILRIMIEKKQIGDEKNKFARK
                     RRRPILEETKTKTKKDKVKIEELDKKLDELLK"
     gene            complement(4141..4503)
                     /locus_tag="P5570_00240"
     CDS             complement(4141..4503)
                     /locus_tag="P5570_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HRR39187.1"
                     /translation="MPYINSTEFGSITIDNKEYGQVLIIGNSIIERDHSKLNKLFGTS
                     HKIGRWEVEKLLEGNPDFILIGTGQSGVMKVDKEVLNKLNKSGAKIIVDITPKIVEDY
                     NQKTKEGKRVNALIHTTC"
     gene            complement(4528..5505)
                     /gene="trpS"
                     /locus_tag="P5570_00245"
     CDS             complement(4528..5505)
                     /gene="trpS"
                     /locus_tag="P5570_00245"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRR39188.1"
                     /translation="MRAFSGIQPSGIIHIGNYFGAIKNWVQLQNEYESIFCIVNQHAI
                     TVPQDPKKLEENTYKVASIYLAFGIDPQKSIIFVQSEVKEHTELCWILNTITRLPELE
                     RMTQFKEKAKQHKSSVNVGLFDYPVLMAADILLYQTDIVPVGQDQKQHVELTITLAER
                     FNKIFGKTFAIPKALIRKEGGKIMGLDDPLKKMSKSASSLFNYIALDDSPAMIREKIK
                     RAVTDSGKEIKYDSQKPAISNLLNIYSLTTNKSTIEIEKEFEGKGYKEFKEALSEALI
                     NFLTPFQKKQKAILKDKNYIRKVLKDGKERAQSIAQKTMIEVKNKIGLV"
     gene            complement(5593..7260)
                     /locus_tag="P5570_00250"
     CDS             complement(5593..7260)
                     /locus_tag="P5570_00250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRR39189.1"
                     /translation="MQNKLKIIPLGGLEEVGKNMTLFEYEGKILIVDMGIQFPDTDMP
                     GVDYIIPNYSYLEKRTKDILGIVFTHGHYDHIGAIPYMINQIGNPPLFATKLAKGLIL
                     KRQEDFSNLAPLNIKEIKREEKISLPPFSIETFPQNHNIPDGIGLLIETPVGRVLHTG
                     DFKFDFTPVADIPTDVSKIASLASKDILLLMSDSTNAEVPGHSLSEKTIQENLGVIFE
                     KSRGRIIAATFASLLSRIQELINLAEIFGRKIIIEGYSMKANVEIAIKLGFLKVKKDT
                     IISPKQLRRLPDNKILIICTGSQGEGDAVLMRLAHKDHRFLSIKRGDSVILSSSIVPG
                     NEKAIQDMKDLLAWQGAKIFHSQMMDIHASGHAQKEELKMMINLTKPKFFMPIHGSYY
                     MREMHKELAMSLNVPEKNIVIGENGSIIELSQEKFEVQKEKAPSTYVMVDGLGIGDVG
                     EIVIRDRQAMAKDGMFVIVIVVDSATGRVKQSPDIISRGFVYLRESKELLRKTRQRVI
                     KLVEKITSSQHPANMAFVKKRIREDLGEFLFKETNRRPMVLPVVIEV"
     gene            complement(7568..7651)
                     /locus_tag="P5570_00255"
     tRNA            complement(7568..7651)
                     /locus_tag="P5570_00255"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7615..7617),aa:Leu,seq:cag)
     gene            complement(7693..9267)
                     /locus_tag="P5570_00260"
     CDS             complement(7693..9267)
                     /locus_tag="P5570_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRR39190.1"
                     /translation="MQNTEIVPADGGIFKEGIIGQPQSLNPLFSMLNDADRDISELIF
                     SGLLAYNSNGDLEKDLAKEYQLSDDGKTYQFTLKDDLLWSDGEPLTTNDIIFTIEAIK
                     DIKVQSPLRLTWQDVKVEKIDEKTIVFTLEAPYLPFLESFTLKILPQHIFQDISPQEY
                     STKPREKIIGSGPFKIKTVEKDKDDKIEKIVLERNQNFYGKIPFFEGVELTFVNSEEE
                     LLKLKNKETALANISPQSKEKLGERYNIYSLFLPRYFALFLNQENKILSQKEVREALA
                     MATPKEDIIEKALLGEGRIVDGPLLEENKIGGEIKKYNFNLEEAKKKLEESHWVDSNN
                     DGIREKILAEGEESTTLELNLLTLPQPQLQETAEILKNTWQEIGVNLNVQSVEGEKLR
                     QDYISQRQYDILLYAQGLYMIPDPSSFWHSTQKKYPGLNFSLYQQSETDDLLKKSIEE
                     INQTKRQELLQSFSKKLTEDIPAIFLYSPNFLYAIDKDVKGIQTKYIVNPSQRFINIE
                     NWYINEKRVPLSSNFE"
     gene            complement(9472..9684)
                     /gene="secG"
                     /locus_tag="P5570_00265"
     CDS             complement(9472..9684)
                     /gene="secG"
                     /locus_tag="P5570_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRR39191.1"
                     /translation="MEILSIFQIIVSILLIVSILLQQRGQGASSIFGGEGGYYFQKRG
                     MEKVFFWSTISLSVLFFILAIANFLI"
     gene            complement(9722..11059)
                     /locus_tag="P5570_00270"
     CDS             complement(9722..11059)
                     /locus_tag="P5570_00270"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRR39192.1"
                     /translation="MKTLKPSQLSSLPRQPGVYLFYGKEKSPLYIGKSSDLRERVKNH
                     FNQPNYKDNLFISQVEKIGYIPLESEIEALILESQLIKKYQPKFNVLFRDDKNYFYVG
                     FTKEDWPRVFITHQPNLKIKNLKLNIEYIGPFVGGTALKETLKILRKIFPYRTCKTLP
                     KKPCLWYELNRCPAPCLIEKELKEKPELKKQLDKRKKDYQKNIKKLKIILKEGKEKLI
                     KDLKKEMEEFAKNEEFEKAAKVRNQITALEGVFSHKIIFYPEKQMEDAGKLLKESLKL
                     PKIPKRIEGYDISHIQGKEMVGSMVVFKLSQIYANNGTNLREYKPDKKEYRRFKIKMV
                     KGQNDVACLKEVIKRRLSHPEWPYPDLIYVDGGKGQFNAVATTLKETGNSKIPVISLA
                     KKKKILYNLISIKPLSLDNLPQVVKITILCLRDEAHRFAIFYHKKLRSHQYIH"
     gene            complement(11511..14534)
                     /gene="uvrA"
                     /locus_tag="P5570_00275"
     CDS             complement(11511..14534)
                     /gene="uvrA"
                     /locus_tag="P5570_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRR39193.1"
                     /translation="MITKFIKIKGARVHNLKNINVNIPRNKLVVITGISGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGIMDKPDCDKIEGISPAIAINQKNISNNPRSTVGT
                     ITEVNDYLRLLFSRIGTPHCPHCKRKIQRQTIDQIIDQILALESSQQKKQTAELLHPL
                     KFSEEKTRQDRNNNNNNNESEIIILGPVIRQKKGEHKGVIEEIERMGFLNVRIDGKLM
                     PIEEAKEITLDKQKKHSIEVVVDRLNILNLNSPKYKIFQREQRSRIADSVETALKIGK
                     GVIIVNRKSKVENEKVTKESFSTNDIIFSEHFACPVCGISLPEMEPRFFSFNSPIGAC
                     HVCSGLGEKLEVDPNLVVPNKNLSLAEGAIYPWARASHKIGRQSYFWWKLQNLASEYN
                     FSVETPFKNLPKNIQKIILYGKDKNSKSNFEGVIPFLERKWGESEGEATREEIEQYMR
                     EKICPACQGKRLKPEVLAVTIKGKSIDDISKMNIKDAKIFFEKLIEGQQQKSKKGKKT
                     SGLTKNQTEIAQPIIKEILNRLSFLEDVGLDYLTLERRGGTLSNGEGQRIRLATQIGS
                     KLVGVTYILDEPSIGLHSRDQGKLIESLKKLRDLENNIIVVEHDPQTILKADYVIEIG
                     PRAGKDGGKIVFEGTPKELLKSSTLTGEYLSQRRKVEIEKSNIKHSISSVQKYLILKG
                     AKEHNLKNIDVKFPLKKLICVTGVSGSGKSTLINDILARALMKKLYRSKTEPGKFGKI
                     MGVENIKRAAIVDQSPIGKTPRSNPVTYIGAFNYIREIFAETEEAKVRGYLPGRFSFN
                     VRGGRCEACQGQGVKKVEMYFLPDIYVECEECKGKRYNQETLEIEYKGKNIADILSMT
                     VDEALSFFRKIPGLERKLRTLKDVGLGYVQLGQPAPSLSGGEAQRVKLAEELSKIESG
                     DTLYILDEPTVGLHSYDIQKLLLVLQRLVDRGNTVIVIEHNLDFIKNADWILDLGPEG
                     GDKGGYIIAEGPPEKIAKNKKSWTGQYLRKIIDVK"
     gene            14734..15207
                     /locus_tag="P5570_00280"
     CDS             14734..15207
                     /locus_tag="P5570_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39194.1"
                     /translation="MKQSFKTGFGFGSTSGVITTLGLIVGLHSGTNSETAVLGGILII
                     AITDALSDAFGIHLSEELNKKNTKREVWESTISTFFSKFLVAFTFIIPVLFLPLFQAI
                     IVSVLWGLVLISFLSFYIAKQQKENSQKVVLEHLSIAIFVVIIAHYLGDLVATFD"
     gene            15235..15597
                     /locus_tag="P5570_00285"
     CDS             15235..15597
                     /locus_tag="P5570_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HRR39195.1"
                     /translation="MGKNKNKEITINDIQKWERSFTKRKGITQNEETAIKIAVFKLIE
                     EVGEVSKAILEEQWDEVPAEVSDVIVFACKVANLAEKFHKTEKLNNVLQRKMNYCETR
                     KYNAKIKKFDKPKNEKFK"
     gene            15745..16926
                     /locus_tag="P5570_00290"
     CDS             15745..16926
                     /locus_tag="P5570_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRR39196.1"
                     /translation="MEKQYLLSEYIHPEEFEYIKKFSQDKETPFLLINLNKVEEKYDE
                     LRHCMPFAKIYYAIKANPNNEVLKVLNSKGSCFDIATIYELDKILALGASPERISYGN
                     TIKKEKDIAYAYKKGVRLFATDSESDLNKICRQAPGSKVFFRLLADGSGADWPLSRKF
                     GAHPDMIFRLALKCKKMNLIPYGLSFHVGSQQRDIGQWDDAIARCRYLFTSLEDADIS
                     LKMINLGGGLPAKYLRPTPSIETYTKEITRFLKEDFGENLPEILIEPGRSIVGEAGVI
                     VSEVIMISQKSTSDHYSWVYLDVGKFGGLIETMDEAIKYPIFTDISQKKLEELIEVIL
                     AGPTCDSMDILYEKFRYRLDKNIKEGDKVFIFSTGAYTASYSSIEFNGIPPLRVYTAR
                     F"
     assembly_gap    17446..17545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17607..17679
                     /locus_tag="P5570_00295"
     tRNA            17607..17679
                     /locus_tag="P5570_00295"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17640..17642,aa:Phe,seq:gaa)
     gene            17728..17799
                     /locus_tag="P5570_00300"
     tRNA            17728..17799
                     /locus_tag="P5570_00300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17761..17763,aa:Gly,seq:gcc)
     gene            17896..19173
                     /locus_tag="P5570_00305"
     CDS             17896..19173
                     /locus_tag="P5570_00305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR39197.1"
                     /translation="MKKKILMVSYRFPWPENKGGYNLRILNFAKILKEEYAVSLLTLV
                     EDKEEEKNIEHLKKENIFEEIIYFYHPRSQEYKNTLQGFFSKLPLQVAYYFSKEIKDY
                     LFKNYHHYDLLYFNTLRAAIYLYQLQSVTNNLPPSVIDLIDSIALNYYDAQKWNKNLL
                     WQLIYQIEIPRLKRFEKKIISDNCFKKVFISSEFDKKYLIQGLETEDRKSKVITIPNG
                     VKEKLFSLPFSLSNASEENWISFLGKMDTQPNQDAVIFFTKEVFPKIYSDPRFSDLYL
                     YIVGISPTRKIEKLEKIKNVKVTGYLKDPYEILKKSKIVVAPLRFGAGIQNKVLEAMA
                     MGKTVVTSPIGARGINKGIPGKHFEVVDSFDPEEWKKKIITLLLDSQKREIIGKEARK
                     LLIDEGYCWKNVGKKILKEINEIYLDYEQRNKK"
     gene            19151..20452
                     /locus_tag="P5570_00310"
     CDS             19151..20452
                     /locus_tag="P5570_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRR39198.1"
                     /translation="MNKEIKNKVLLLIGDVFFLYLSLFFVLLIRYREIPQSPSLKDFF
                     LYFSWLFLLWLFFLWMFDFYSLKLRAGSLEFFRFLSIFIFLCLLSGALYFYLQPNLPV
                     SPKAIFILDIVIFFLFFVGWRYFFDFVFQKRTKKEKVLILGNPPEIEDLLFFLKKFPS
                     YYTVKVIPEIDKEKLLEISDILKKENPQKIIIANHLEFLANISFFSHIPIESFAHFYE
                     EITQKVALSALNDVRFLEDFYKSEERSYVILKRIFDIFLSLIGLLILGVLFPFIALAI
                     KLDSPGPVFFTQERVGKERKVFCFYKFRSMYASPREKTDLWREKDKKEITKVGRFLRV
                     THLDELPQVINILKGDISFVGPRPEWKKIAEKLEKDIPFYFLRYRVQPGLSGWAQINI
                     PPSTSIQEAKEKFQYDLYYLKHRSFLFDLVIFLKSLRKIFG"
BASE COUNT         6660 a         3142 c         3325 g         7587 t
ORIGIN      
        1 tcttgaaaac cattgtggcc aaaagccacc gtgagttcga atctcacccc caccgttacg
       61 taaaaattct ttcaggattt ttacgtatcg cgtgcataat tcaaatcatt tactcgttct
      121 catcttttaa aaataaagga gggggtaata tttgtaacct taattttgtt ctaaatttcc
      181 taaaatttaa ttgactttga taagaaaatt tgctatgttt aatttctatg agatcaggaa
      241 aatatgatga caaaaacgct tttctttcaa tttatgcagg aacgggtgga aaagatgctg
      301 aggattgggt gagtattctt tgccgaatgt accaacgtta tcttcaaaaa aaaggattaa
      361 agacaaaaga aattcaaaaa atatcaggag aaggaggtat taaatcagtg acatacgagg
      421 ttaaaggaaa aggtgcttac ggacttttaa aaggagaaaa tggcgttcat cgattagtac
      481 gtatttcacc tttttcggct aaaaaccttc gtcatacctc gtttgctttg gtagaggtgt
      541 tgccagaaat agaaacagaa gaaattaaaa ttcaagacaa agatttaaaa gtagaaacat
      601 ttaggtcttc aggcgctgga ggacaatatg tgaatcgtcg agaatcagca gtaaggatag
      661 ttcatttacc aacaggcatt agtgtaactt cccaggaaga aagattacaa ggcgaaaaca
      721 aaaaaagagc ccttcaaatt ttacaagcaa aattacttca aaaaagagaa gaagagaatg
      781 cagaaaaaat agaaaacgaa agaggagaat tgccattggc tgactgggga agacaaatta
      841 gatcttatgt ttttcatccc tatcagatgg taaaagatca tcgaacaggc attaaaactt
      901 ctaatctgaa aaaagtatta gatggggact tggataaatt tataaaatag tatgttgaga
      961 gttgattgta gattgtagaa acgactacct acttttaact ttttactttc gagtttatga
     1021 tttattttaa taatgttaca aaggtttttt ctaataattg cgtggctttg tctgatataa
     1081 gttttggggt ttcagatgga gaatttgttt ctattgctgg aaaatctgga gcaggaaaat
     1141 caacactgat tagattacta ttaaacgagg aaagaccaac tcagggagag attatttttg
     1201 aagatattaa tgtaaataaa atgagtccat catatctccc ttattatcgg cggaaaatag
     1261 gtgttgtata tcaggattat aaacttcttc ctcaaaaaac ggtttttgag aatgttgctt
     1321 ttgctttgga ggtattgggg aaaagcgaga gtgaaataaa aagagtagta ccccaagcat
     1381 tggatttagt agggattttg gataaagcat caaattttcc aaaagaatta tctggaggag
     1441 aacagcaaag aacagctcta gccagagcta ttgtaattca accaagggtt cttgtggctg
     1501 atgaaccgac aggaaatctt gatatgatta ccactaaaga aatagtaaaa ttatttttaa
     1561 aaattaatca attagggaca gcagtaatat taactacaca taatcgtgaa attgttaata
     1621 gtattcaaaa aagattaata gtattagata aaggaaaaat tataagagac gaagaaaagg
     1681 gaagatacta cctttaataa aaatttttaa gcacaaaatt ctaaattcta aacttcaaat
     1741 tatttaggat ttaaaaatta ggatttagaa attttttgca gagtaaaaag ttttatatgt
     1801 ttaataattt ttttagagtt tttaaattcg gttggcagga gatttcaagg aacatgggga
     1861 tctctattgg aacaattttt attattttta ttgccttgtg tttggtgggc ggaacctttc
     1921 ttttacgcgg aatgagtgaa aatctcataa caacactgga aaataaagta gatgtaagtg
     1981 tatatttcaa agacgacgca aaagaagaag atgttctttt ggtaaaaaaa caaatagaag
     2041 aatttcctga ggtaaaagaa gtagaatata tttcaaaaga aaaagccctt gaaactttta
     2101 aagaaacaca taaaaataat caacttttaa tggaaagttt aactgagatt ggcagcaatc
     2161 ccttaccgtc ctctttaaat attaaagcta ttcaagcttc atcgtatgaa aatttaacaa
     2221 actttttgat gaatggagag tataaaaaca ttattgaaaa aataaattgg agtgaaaacc
     2281 aagctattat tgaacgactt ttttcaattt ctaatgctgt aaaaacagga ggatttgcgg
     2341 ccagtttaat tttggtttta attgctgtta ttgttacctt taacacaata agattagcta
     2401 tttattctaa acgagaagag attgagacaa tgaaacttat tggagctact aattggttta
     2461 ttagagggcc ttttgtggtg caaggattaa ttacaggatt ttttgctggt cttattgctt
     2521 ttcttttctt ttatggagta gagatgggtc tttcgtctcg aggattgggt atttttggag
     2581 aattaggatt ttttaacttt ttcgaagaaa atattttgtt gtttcttatt attcaaattg
     2641 gcggtggtat aattattagt acttttgcaa gtttacttgc ggttcaaaga tatcttaaga
     2701 cagaaaaaaa ctaacaaaat caagattttt tgttagtttt taattatata aaattagtta
     2761 taaattattt tatataaggt aaaatagcca tctctcttgc ttttttgatg gcttttttta
     2821 atttacgttg atgggcattg caaacgcctg ttttttttct tgattttatt ttggcacctg
     2881 cagaaatata acgagacagt aattgctcgt ttttccaatc aatttcatta atgtttcttt
     2941 gacaaaaata gcaagccata gttcgttttt taaattataa tgcttaatgt ttgattaagc
     3001 tttaataatt aaatgactaa aaattaaaat aggtttttta tcaattagat taattttgga
     3061 tcattatagt ggtaaatcct ttacatctat ttcttcctct tcttcaataa taggaatttc
     3121 ttcttctttt atttctggct gaggtggttg taccgaagga gcaggtcttt gatagaaagc
     3181 agaggactga ttcttgggac caagctgaag catttctgta ataatttcag ttctgtaccg
     3241 ttttattcca tctttttccc aactgcgggt ttggagatgt ccttctatta aaacaatatc
     3301 gccttttttc agatactgaa ccgctgtttc agccaatttt ccaaaagcta ctacattatg
     3361 aaattctgtt tgttcttgtt tttctctgtt tttatcataa aatactcgat ttgttgccac
     3421 gctaaaattt gttactgttt gaccagaagg aagtgatttt gcctgaagat cttgtgttac
     3481 tcgtcctaaa ataaaaactt tatttagatt catatatgta agttccgctc cacagagcgg
     3541 aacgaagcgt ggagacggag ataaacgtca tcaacttact tatttattta agtagttcgt
     3601 ctaatttttt atctaattct tcgattttta ccttgtcctt ttttgttttt gtttttgtct
     3661 cttctaatat cggtcttctc cttttccttg caaatttatt tttttcatcg ccaatttgtt
     3721 ttttctctat cataattctt aaaatatttt cattcatctt taaaaaatca tttaattctt
     3781 ttattttact tccttcaatt gtaaactgtg ttactcctaa aaaccctact ctttgttttt
     3841 ttactgggta ggcaaataat ctttttttaa ttagctgatc ttgaatcgtc ccgtttaatt
     3901 ttttaatctt ttcttctatc gttttaagaa gatcttcttt ttcttttgta tccaaattag
     3961 gtgttaaaaa atcaagttcg taattcataa atttgaattt aaaatattac tcttttttaa
     4021 acatttttac ccttaatata ttattatagc aaaaaaataa aaatttatca agaaaataga
     4081 cgatttttta aaaggttcaa aaaatgatag ggataaacac ttctgtttta atttttaata
     4141 ttagcaggta gtgtggatta gcgcattgac tctttttcct tcttttgtct tttggttata
     4201 atcctcaaca atttttggtg taatatcaac aattattttg gcaccacttt tgtttaattt
     4261 atttaaaact tctttatcta ccttcattac tccagattga cccgtgccta tcaaaataaa
     4321 atcaggattt ccttcaagta atttttccac ctcccatctt cctattttat gagatgtacc
     4381 aaataattta ttaagttttg aatgatctct ctcaatgatt gagtttccaa taattaaaac
     4441 ttgaccatat tctttattat caatggtaat agaaccaaat tcggttgaat taatgtaggg
     4501 cataaaaaca caacttaaaa ttaaaaatta aactaatcct attttatttt ttacttcaat
     4561 cattgttttc tgggcaatac tttgtgccct ttcttttcca tcttttaaaa cctttcgaat
     4621 ataattttta tctttcaaaa ttgctttttg tttcttttga aagggggtaa gaaaattaat
     4681 caatgcttca ctaagcgctt ctttaaactc tttatatcct tttccctcaa attctttttc
     4741 tatttctatg gtgcttttgt tggttgttaa actgtaaata tttaaaagat ttgagattgc
     4801 tggtttttga gagtcatatt ttatctcttt tccagaatca gtaactgccc ttttgatttt
     4861 ttctctaatc attgctggtg aatcatcaag agcaatgtag ttaaagagag aggaagcgct
     4921 ttttgacatt ttctttaagg gatcgtctag ccccataatt tttccgccct cttttctaat
     4981 aagtgctttt ggtattgcaa aggtctttcc aaatatttta ttaaatcttt ctgctagggt
     5041 tattgttaat tcaacatgtt gcttttgatc ttgacccact ggcacaatat cagtttgata
     5101 caataaaata tcagcagcca ttaaaactgg ataatcaaaa agaccaacat tgacacttga
     5161 tttgtgttgc tttgctttct ctttaaactg ggtcattcgt tcaagctcgg gtaagcgggt
     5221 aatagtattt aaaatccaac aaagttctgt atgctctttt acttctgatt gaacaaaaat
     5281 gattgatttt tggggatcaa tgccaaaagc aagataaatt gaagcaacct tataagtatt
     5341 ttcttcaagt tttttgggat cttgaggcac agtaatagcg tgttgattaa caatgcaaaa
     5401 aattgattca tactcatttt gaagttgaac ccagttttta attgccccaa aataatttcc
     5461 aatatgaata ataccgcttg gttgaatacc agaaaaagcg cgcataaata ttattttaaa
     5521 agaatcattt taagaaccat gatagcgatg gatattatac ttaaaatgaa ctttcttatt
     5581 ttattaagat ttttatactt cgattacgac cggtaaaacc attggtcgac gatttgtttc
     5641 cttgaaaaga aactctccta agtcctctct gattcttttt ttaacaaatg ccatattagc
     5701 agggtgttgt gaagaggtaa ttttttctac tagtttgatt actctttgac gggtttttct
     5761 taacaattct tttgactctc gaagataaac aaaacctctt gaaattatat caggactttg
     5821 tttaaccctt ccagttgcag agtccaccac tattactatt acaaacattc cgtcttttgc
     5881 cattgcctgt ctgtcgcgta tcactatttc tcccacatct cctattccaa gtccgtcaac
     5941 cattacataa gttgatggag ctttttcttt ttgtacttca aacttttctt ggcttagttc
     6001 gatgatgctg ccattttctc caataacaat atttttttca ggaacattca atgacattgc
     6061 tagttctttg tgcatttccc gcatataata actgccgtga atgggcataa agaattttgg
     6121 ctttgtaaga ttaatcatca ttttaagttc ttctttttga gcatgtcctg aggcatgaat
     6181 gtccatcatt tgagaatgaa aaatttttgc tccctgccaa gccaaaagat ctttcatatc
     6241 ctgaattgct ttctcattgc ctggaacaat agaagaagaa agaatgacac tatctcctct
     6301 ttttatagaa aggaatcgat ggtctttatg agcaagacgc attaatactg cgtctccttc
     6361 tccttgagat ccggtacaga taattaagat tttattatct ggaaggcgac gcaattgttt
     6421 gggagaaata atagtatctt ttttgacttt taagaatcct aattttattg caatttctac
     6481 atttgctttc attgaatatc cttcaataat gatttttcgt ccaaaaattt ctgccaagtt
     6541 tattaactct tgaattcgag aaagcaaaga agcaaaagta gcggcaataa ttcttcctcg
     6601 gcttttttca aaaataactc ccaggttttc ttgaatggtt ttttctgata aactatgtcc
     6661 gggaacctcg gcattagtag aatcagacat taaaagaaga atatcttttg atgccaagga
     6721 agcaatttta gagacgtcgg tgggaatgtc agctactggt gtaaagtcga acttaaagtc
     6781 accggtgtga agtactctgc caacaggtgt ttctattaaa agtcctattc catcaggaat
     6841 attatgattt tgcggaaagg tttcaataga aaagggagga agagaaattt tttcctctct
     6901 tttaatttct ttaatattta atggtgcaag attggaaaaa tcttcttgtc ttttcaaaat
     6961 taatcctttg gcaagttttg ttgcaaagag aggcggattt cctatttgat taatcatata
     7021 tggaatagca ccaatatgat cataatgacc atgagtaaaa acaatcccaa ggatgtcttt
     7081 ggttcttttt tcaagataag aatagttggg aataatataa tcaactcctg gcatatcagt
     7141 atcaggaaat tgaatgccca tatcaacaat taaaattttg ccctcgtatt caaaaagggt
     7201 catatttttt cctacttcct ctagtcctcc cagaggaata attttgagtt tattttgcat
     7261 aaatttataa ttctaaaatt tgtctattgt ttaatgatag accgtattaa gaatttgtta
     7321 agcaggttca agtttctaac aagcgttttt aaaaaatata ttcctttaag ccaagggttt
     7381 atattctaaa cttttttcga ggctgacacg taatggcaga tatattaatt ttgtatttct
     7441 ctaccaaaag ataacacgtt aaactaccaa tttacgctta gcgcagaaag gtattttttg
     7501 attcattaaa ctagcttagg aaatattttt ttgaattgtg ttaaattctt gaaaataaat
     7561 taagttatgc ggacggtgag agtcgaactc acaggggatt gctcccagta gctcctgaag
     7621 ctaccgcgtc tgccaattcc gccacgtccg cttgctatta atacaaatta tcgcattatt
     7681 agcaaacatt gattattcaa aatttgagga gagtggcact cttttttcat taatgtacca
     7741 attttcaata ttaataaatc tttgagaagg atttacaata tattttgttt gaatgccttt
     7801 tacatcttta tctatagcgt aaagaaaatt tggactataa agaaatatag caggaatatc
     7861 ttctgttaac tttttagaaa aggactgaag caattcttga cgtttcgttt gattgatttc
     7921 ttctattgat tttttaagca gatcatctgt ttcgctttgc tgatataatg aaaaatttaa
     7981 accaggatat tttttttggg ttgaatgcca gaaagaggaa ggatcaggaa tcatataaag
     8041 tccttgagca taaagcaaaa tatcatattg tctttggcta atataatctt gacgtaattt
     8101 ttcgccctct actgattgaa catttaaatt tacaccaatt tcttgccagg tatttttcaa
     8161 gatttctgct gtttcttgaa gctgtggctg gggaagagtt aaaagattta attcaagggt
     8221 tgttgattct tctccctctg ctaagatttt ttctcttata ccatcattat ttgaatcaac
     8281 ccaatgtgac tcttctaatt tttttttggc ttcttctaga ttaaaattat attttttaat
     8341 ttcgcctccg attttatttt cttctagaag gggtccgtct actattcgtc cttcacccaa
     8401 taatgctttt tcaataatat cttcttttgg ggtagccatt gccaatgctt ctcttacttc
     8461 tttttgagaa aggattttgt tttcttgatt taaaaataaa gcaaaataac gaggcaaaaa
     8521 aagagaataa atattatacc tctctcctaa tttttcttta ctttgaggag aaatattagc
     8581 cagagcagtt tctttatttt ttaattttaa aagttcttct tcgctattaa caaaagttaa
     8641 ttcaactccc tcaaaaaaag gaatttttcc gtaaaagttt tgatttcttt caagaacaat
     8701 tttttcaatt ttatcatctt tatctttttc tactgttttt attttaaatg gtcctgatcc
     8761 aataattttt tctcttggtt ttgtggaata ttcttgagga ctaatgtctt gaaagatgtg
     8821 ttgaggcaaa atttttaagg tgaaactttc aaggaaagga agataagggg cctctagtgt
     8881 aaagacaatt gttttctcat caattttttc tactttaaca tcttgccagg taaggcgcag
     8941 aggactttga acctttatgt ctttgatagc ttcaatagta aatatgatat cgttggtagt
     9001 aaggggttca ccatcagacc agagaagatc atcttttaaa gtaaattgat aagtttttcc
     9061 atcatcactc aattgatatt ctttggcaag gtctttttct agatcaccat tgctattgta
     9121 agcaagaagt ccagaaaaaa ttaattctga aatgtctcga tcagcatcat ttaacattga
     9181 gaagagggga tttaatgatt gaggttgtcc aataattcct tctttaaata tcccaccatc
     9241 agctggtaca atttctgtat tttgcaataa agaaagtctc caccagtaaa tacatgaagc
     9301 aataataatt attattaaaa aaataaaaat aattttttca gtttttgaaa taacgttaaa
     9361 aaaagaaaac cattgttttt ttgatggcca tttaccattt attcttttcc atgcatctaa
     9421 atgaaaaatt ctggtaaaaa aattttggga gggaatacta atatgtttat attagattaa
     9481 aaagtttgcg attgctaaaa taaaaaaaag aactgaaaga gaaatagtag accagaaaaa
     9541 cactttttcc atacctcttt tttgaaaata atatccgcct tctccgccaa aaatagaaga
     9601 ggcgccttga cctcgctgct gtagaagaat cgaaacaatc aataaaattg aaacaataat
     9661 ctgaaaaata gataaaattt ccatataaac tcacattttc actaatcatt tgcgaattaa
     9721 attagtgaat atattggtga gatcttagtt ttttatgata aaaaatagca aaacgatgtg
     9781 cttcgtctct taaacaaaga atagttattt taacgacttg aggaagattg tcaagagaga
     9841 gtggctttat agaaattaaa ttatacaaaa tttttttctt ttttgcaagg gagatcactg
     9901 gtatttttga atttcctgtt tctttaaggg ttgttgcaac agcattaaat tgccccttgc
     9961 caccatcaac atagattaga tcaggatatg gccattcagg gtgagacaat cttcttttga
    10021 taacctcttt tagacaggca acgtcatttt gtccttttac cattttaatt ttgaaacggc
    10081 gatattcttt tttatcaggt ttatattcgc gtaaatttgt tccattgttc gcgtaaattt
    10141 gtgaaagttt aaaaactacc attgaaccaa ccatctcttt tccttgaata tgagaaatat
    10201 cgtatccttc aattcttttt ggaattttag gaagtttcaa agattctttt aaaagtttcc
    10261 ctgcatcttc catttgtttt tcaggataga aaattatttt atgagaaaaa actccttcaa
    10321 gagctgttat ttggtttctc acttttgctg ctttttcaaa ctcttcattt ttggcaaatt
    10381 cttccatttc tttctttaaa tctttaatca atttttcttt gccttctttt aaaattattt
    10441 ttaatttttt aatatttttt tgataatcct tttttctttt gtctaattgt ttttttaatt
    10501 ctggtttttc ttttaattct ttttcaatca aacaaggagc agggcagcga ttgagttcgt
    10561 accaaagaca aggtttttta ggcaaggttt tgcatgttcg atagggaaaa atttttctta
    10621 atatttttaa ggtttccttc agggcagtac cacctacaaa agggccaata tattcaatgt
    10681 tcaattttaa atttttaatt tttaaattgg gctgatgggt gataaaaact cttggccagt
    10741 cttccttggt gaatccaaca taaaaataat ttttgtcatc cctgaaaaga acattaaatt
    10801 ttggctgata ttttttaatg agttgacttt ccaagattaa agcctctatt tctgattcta
    10861 aaggaatata accgattttt tcaacctggc tgataaaaag attatcctta taatttggct
    10921 gattaaaatg gttttttact ctttctctta aatcagagga cttaccaata taaagagggg
    10981 atttttcttt accataaaaa aggtaaacgc ctggttggcg cggcaaagaa gaaagttgag
    11041 aaggttttaa ggttttcatt ttcatttgat ttattttatc atattttatt gtaggcgcaa
    11101 ccttcagctc aataagtaag taaatatgcc gagaaacgtt ctcggcatat tttattttat
    11161 tacaggcgca accttttgtc ttgacaaatt aaggcgtatt tggtataata gggccggagg
    11221 tgaaaaaatg gaaatcgatt gggattggag agacgccgct attatagcgg tatttctcct
    11281 agcgggcgtg gctggcctga tggccatcat tcacgccctc ttcttccggc tgggaacagt
    11341 agggcaagct cttttatggg gaatcttttt tactcttatt tttttaagga ttctccttaa
    11401 gaagagtgga gtagggtaag taaacaagac ccgagcatgt ctgaaaactg ctcttttttt
    11461 ttatcaaaaa taattgtgcc gagaaacgtt ctcggcacaa ttctcattca ttatttaaca
    11521 tctattattt tcctaaggta ttgtccagtc caactttttt tgtttttagc aattttttct
    11581 ggtggacctt cggctataat ataaccacct ttatcaccac cctctggtcc cagatccaaa
    11641 atccaatcag catttttaat aaaatctaag ttgtgttcaa ttactattac tgtatttcct
    11701 cgatcaacca atcgttgcaa aaccaaaagc aatttttgaa tgtcataact atgaagtcca
    11761 actgttggct catctagaat ataaagcgtg tcaccgcttt caatttttga tagttcttcg
    11821 gctagtttta ctcgttgtgc ttcacctcct gacaaactgg gtgctggctg gcccaattga
    11881 acataaccca atcctacatc ttttaaggtc cttaattttc tttccagtcc cggaattttt
    11941 cgaaagaatg aaagcgcttc atcaaccgtc attgaaagaa tatcagcaat attttttcct
    12001 ttgtattcta tttctaaggt ttcttgatta tatctttttc ctttgcattc ctcgcactca
    12061 acataaatat ctggtaaaaa atacatttct acttttttta caccttgtcc ctgacaggcc
    12121 tcgcatctac cacctcttac attaaaagaa aacctgcccg gcagatatcc tcttactttt
    12181 gcttcttcgg tttcagcaaa aatctctctg atataattaa aggcaccgat ataagttacg
    12241 ggatttgagc gtggggtttt gcctatcggt gattgatcaa cgattgccgc tctcttaata
    12301 ttttccacac ccataatttt accaaacttt cccggttctg tttttgagcg ataaagtttt
    12361 ttcatcagtg cccgcgccaa aatatcattt atcaaggttg attttcctga gccagaaaca
    12421 cccgtcacgc agattaattt ttttaaggga aattttacat caatattttt aagattatgt
    12481 tcttttgcgc cttttaaaat tagatatttt tgaacgcttg atattgaatg cttgatgttg
    12541 gatttttcaa tctctacctt tcttctttga gataaatatt caccggttaa agtgcttgat
    12601 tttaaaagtt cttttggtgt cccttcaaaa acaatttttc caccgtcttt tccagctctt
    12661 ggtccaattt caattacata atctgccttt aaaatggttt ggggatcatg ttcaacaacg
    12721 atgatgttgt tttctaaatc tcttaatttt tttaaacttt caattaattt tccctgatca
    12781 cggctatgaa gaccaattga tggttcgtct aaaatataag tgacaccgac caattttgaa
    12841 ccaatctggg tggctaatct aattctttgg ccttctccat tagaaagagt cccgccgcgc
    12901 ctttccaggg tcaaataatc aagtcctaca tcttctaaaa aagacagccg attaaggatt
    12961 tccttaataa ttggttgagc aatctctgtt tggttttttg tcaagccgct agtttttttt
    13021 ccttttttag atttttgctg ttgtccttca atcagttttt caaaaaaaat tttagcatct
    13081 ttaatgttca tttttgagat atcatcaatt gattttcctt taatagtaac agctagcact
    13141 tctggtttta atcgtttgcc ttgacaagcc gggcaaattt tttcacgcat atattgttca
    13201 atttcttctc gtgttgcttc gccctctgat tctccccatt ttctttctaa aaatggaata
    13261 actccttcaa aattagattt tgaatttttg tctttgccat ataaaattat tttttgaata
    13321 ttttttggca agtttttaaa aggtgtttct acagaaaaat tatattcaga tgctaagttt
    13381 tgaagcttcc accaaaaata ggactgtctt ccgattttat gagaagcacg tgcccaggga
    13441 tagattgctc cttctgctaa agacaaattt ttattgggaa caactaggtt aggatctact
    13501 tctaattttt ctcccaatcc cgagcaaaca tgacaagcgc ctattggaga attaaaagaa
    13561 aaaaatcgtg gttccatctc tggtaaagaa ataccacaaa ctgggcaggc aaaatgttcg
    13621 gaaaaaataa tgtcgttggt agaaaatgat tctttagtta ctttttcatt ttctactttt
    13681 gattttctat ttacgattat aacgcctttt ccaattttta aagctgtttc tactgaatcc
    13741 gcaatcctcg aacgttgttc tcgctgaaat attttatatt taggactatt gagatttaaa
    13801 atatttaatc tgtcaaccac cacttcaatt gaatgctttt tttgtttatc taatgttatt
    13861 tcctttgcct cttcaatcgg catcaatttt ccatcaattc ttacatttaa aaatcccatt
    13921 ctttcaattt cttcaatcac gcctttatgc tctccttttt tttgacgaat aactggtccc
    13981 aaaattatta tttcggattc attattattg ttattgttat tgcgatcctg tctagttttt
    14041 tcttcagaaa atttgagtgg atgaagcaat tctgctgttt gttttttttg ctgagaagat
    14101 tctagtgcca aaatttgatc aatgatttga tctatggttt gccgttgaat ttttctcttg
    14161 cagtgcggac aatgtggagt gccaattctg gaaaataaaa gccggagata atcattaact
    14221 tcagtaatgg ttccaactgt tgaacgggga ttattgctaa tatttttttg attgattgca
    14281 atggcaggtg aaattccttc aatcttatca cagtcgggct tatccattat gcccaaaaat
    14341 tgtcttgcat aggcagatag gctttcaaca taccttcttt gcccttcagc atacaaggtg
    14401 tcaaaggcca atgaagattt tcctgaacca gaaataccag taattactac taatttattt
    14461 cgaggaatat taacattaat attttttaaa ttatgaaccc gtgccccttt aattttaata
    14521 aactttgtaa tcataaataa tttatatatt agaaaaaatc aatttaatta aaaaattatt
    14581 ttaccttcta ttttaacact atctaagata aattttcaac aatccctggc aatttcagtc
    14641 ttgttgacac tttaattacg cttatcttgc aaatcaaaag agattgggat aaactataac
    14701 tataaagtaa ctgataattg atatatgttc aatataaagc aatcatttaa aaccggtttt
    14761 ggttttggtt caacttcagg agttattact accctaggtt taatagttgg tttacattct
    14821 ggcacaaatt cagaaacagc agtacttgga ggtattttga ttattgctat tactgatgca
    14881 ctctctgatg ctttcggaat tcacctatcc gaagaattaa acaaaaagaa tacaaaaaga
    14941 gaagtatggg aatccactat ttctactttt ttttctaaat ttttagttgc ttttactttt
    15001 attattcctg ttttgttttt acccttattt caagcaatta ttgttagcgt tttgtgggga
    15061 ttagtattga ttagttttct tagtttttat atcgccaaac agcaaaaaga aaattctcaa
    15121 aaagttgttt tagagcatct ttcaattgct atttttgttg ttattattgc tcactatctt
    15181 ggtgatttgg tagcaacctt tgattaaaca aatctaatta aaaaataatt acatatggga
    15241 aaaaataaaa ataaagaaat cacaatcaac gatattcaga aatgggaaag gtcatttact
    15301 aaaagaaagg ggattactca aaatgaagaa accgctatta agattgctgt ttttaagtta
    15361 atagaagaag taggggaagt gtctaaggct atattagaag aacaatggga tgaagtccca
    15421 gctgaagttt ccgatgttat tgtttttgct tgtaaagtag ccaatctagc agaaaaattt
    15481 cacaaaaccg aaaagttaaa taacgtatta caaaggaaaa tgaattattg tgaaactaga
    15541 aaatacaatg ccaaaatcaa aaaattcgat aagcccaaga acgaaaaatt taaataagtg
    15601 ataaaattat atttgatctt gctgaatatt taatcttgcg ttataataat attgcatatg
    15661 gtagcgattt ttagaagtta aagtttcgac cttttactaa tctttttatt ctaccatatg
    15721 catttttatt aaaataaaaa ttttatggaa aaacaatatc ttctttctga atatattcat
    15781 cctgaagagt ttgaatatat taaaaaattt tctcaagata aagaaacacc gtttcttttg
    15841 attaacctta ataaagtaga agaaaaatat gatgaattgc gacattgtat gcctttcgca
    15901 aagatatatt atgcgattaa ggccaaccca aataatgaag ttttaaaagt tcttaattct
    15961 aaggggtctt gttttgatat agctacaatt tatgaactag ataaaatttt agccctgggt
    16021 gcttcgccgg aaagaattag ttatggcaat actataaaaa aagaaaaaga cattgcctat
    16081 gcttataaaa aaggtgttcg actgtttgct actgattcag aaagtgattt aaataaaatt
    16141 tgccgtcaag cacctggatc aaaagttttc tttcgtttgc tagccgatgg tagtggggct
    16201 gattggccac tttcgcgtaa atttggagcg catccggaca tgatatttcg tttggcgctt
    16261 aagtgtaaaa agatgaactt aattccttat ggtctttcct tccatgttgg ttctcaacaa
    16321 agagatattg gtcaatggga cgatgctatt gctcgatgtc gctatttatt tacttctttg
    16381 gaagatgcag atatatcttt aaaaatgata aatttaggag gaggattacc ggctaaatat
    16441 cttagaccaa cacccagtat agaaacttat accaaagaaa ttacaagatt tttaaaagaa
    16501 gattttggag aaaacttacc tgaaatttta atagaacctg gccgatctat tgtaggtgag
    16561 gcaggggtaa tcgtttctga ggttataatg atctctcaaa aatcaacttc agatcattat
    16621 agctgggttt atttggacgt tgggaagttt ggaggtctta ttgaaacaat ggatgaagca
    16681 ataaaatatc ctatttttac tgatatatcg caaaagaaat tagaagaatt aatagaagta
    16741 attttagctg ggccaacctg tgacagtatg gatattttat atgaaaaatt tcgttatcga
    16801 ttagataaaa atataaaaga aggagataaa gtttttatat ttagcactgg tgcttatact
    16861 gctagttata gctcaattga atttaatggt attccacctc ttcgtgtata taccgcccgt
    16921 ttttaaatgc aaagaaacgt tcatagatac ccttctttat tttttaaaaa agcaaaactt
    16981 aacaactatg aacttgtggt tattatgaat gattttatgg tacaagaaaa ttaccggaat
    17041 atttaggtta caaactagtc aaatatttag caggttgagt gtaaaatttt tccaaattta
    17101 acacttttag atattcttaa aaagaattta aaggaattgt caaaatttaa taaagttaaa
    17161 ttaaaaataa aatgaacttc aaaaaaataa tttatatttt gttgacgaca atgcttgggt
    17221 taattttatc tttaattttg catgggataa ttgagattta ttatattaat tttcttttaa
    17281 aagaagatat tttgccacag tctagcacat taattcctca atgttatttg ccttccttat
    17341 tgcagatttt tttattattg ttaggtctta ttggtggtta ttttcttggt catttttggt
    17401 ggaaaaagat atatgaaaat aaaatataaa taaacaaaaa ttaaannnnn nnnnnnnnnn
    17461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnntcaca agacttgctt taaagagata agtttgataa
    17581 aatatttgac aaataaaaag tttaaagccg tggtagctca gttagtagag catagctctg
    17641 aagaagctgg ggtcggcagt gcaattctgc cccacggcac aatcgttaat attaaaattt
    17701 tatatttgtg atttaaaatt ttaatatgcg ggtgtagtac agtggttagt acatgacatt
    17761 gccaatgtca agacggcggt tcaattccgc tcacccgcta ttttttaaaa aaaacttaga
    17821 agaaaaagaa aatgtacttt tctttagtta gaactagctt ttttaaagac tttaaaagca
    17881 aacgaaattt cttttatgaa gaaaaaaatt ttgatggttt cttatcgctt cccatggcct
    17941 gaaaataagg gagggtataa tcttcgaata ttgaactttg ccaagatatt aaaagaagaa
    18001 tatgctgttt ctcttctgac cctagtagaa gacaaagaag aagaaaaaaa tatagagcac
    18061 ttgaaaaaag agaatatttt tgaagagata atttattttt atcatccacg atctcaagaa
    18121 tataaaaata cattgcaagg ttttttttca aaattacctt tacaggttgc ctattatttt
    18181 tctaaagaga taaaagatta tctttttaag aattaccatc attacgatct tttatacttt
    18241 aacactctta gggcagctat ttatttatat cagctgcaaa gcgttacaaa caatcttcct
    18301 ccttcagtga ttgatcttat tgactctatt gctcttaatt attatgatgc tcaaaaatgg
    18361 aataagaatt tattgtggca gttgatttat caaatagaaa ttccacggtt gaaaagattt
    18421 gaaaaaaaaa ttatcagtga taattgcttt aaaaaggttt ttatttcctc tgaatttgat
    18481 aaaaaatatt taattcaagg gttagaaact gaagatagaa aatcaaaagt aataacgatt
    18541 cctaacggtg taaaagaaaa acttttctct cttccgtttt ctttatctaa tgcttcagaa
    18601 gaaaattgga tttcttttct tggtaaaatg gatactcagc caaaccaaga tgcagtaatc
    18661 ttttttacaa aagaggtatt tcccaaaatt tattcagatc cgcgtttttc tgatttgtat
    18721 ctttatattg ttggtataag tccaacaaga aaaatagaaa aattagaaaa gataaaaaat
    18781 gtaaaggtta ctggttacct caaggatcca tatgaaatat taaaaaaatc aaaaatagtt
    18841 gttgcaccat tgcgatttgg agctggaatt caaaataagg ttttagaggc aatggcaatg
    18901 ggaaaaacag tagttacttc acctattggt gcaaggggaa ttaataaagg aataccagga
    18961 aagcattttg aagtagttga ttcttttgat ccagaagagt ggaagaaaaa aattataaca
    19021 ttattattgg attctcaaaa acgcgaaata attgggaaag aggcaagaaa attattgatc
    19081 gatgaaggtt attgctggaa aaatgttgga aagaaaatat taaaagaaat aaatgagatc
    19141 tatttggact atgaacaaag aaataaaaaa taaggtcttg ctattgatag gtgatgtttt
    19201 ttttctttat ctctctctct tttttgtttt actaataaga tatagagaaa ttccacaatc
    19261 tccttctttg aaagatttct ttttatattt ttcgtggctt tttttattgt ggttattttt
    19321 tttgtggatg tttgattttt attctttaaa attgcgagcg ggaagtttag agtttttccg
    19381 atttctgtca atttttattt ttttatgtct tttatcagga gctctttatt tttatctcca
    19441 acctaatttg ccggtttctc caaaagcaat tttcatttta gatattgtaa tttttttctt
    19501 gttttttgta ggatggcgat atttttttga ttttgttttt caaaaacgaa caaaaaaaga
    19561 aaaggtatta atcttgggca atcctccaga aattgaagat ttgctgtttt ttttaaaaaa
    19621 attcccctct tattatactg taaaggtgat tccagaaatt gataaagaaa aattactaga
    19681 aatatctgat attttaaaga aggaaaaccc tcaaaaaatt attattgcca atcatcttga
    19741 atttttagca aatatttctt ttttttcaca tattcccatt gaaagttttg ctcattttta
    19801 cgaagagata actcaaaaag ttgctctttc agcgctaaat gatgtaaggt ttttagagga
    19861 tttttacaag agcgaagaac gatcatatgt aattttaaaa agaatttttg atatttttct
    19921 ttctttaatt ggtcttttga ttttaggtgt gttatttccg tttattgctt tagcgataaa
    19981 acttgattct cctgggccag ttttttttac tcaagaaagg gtaggaaagg aaagaaaagt
    20041 tttctgtttt tataaatttc gttctatgta cgcttctcca agggaaaaaa ctgatttatg
    20101 gagagaaaaa gataagaaag aaataacaaa agtaggaaga tttttgagag taacccatct
    20161 cgatgaatta ccgcaagtga ttaatatttt aaaaggagat attagttttg ttggacctcg
    20221 gccagaatgg aaaaaaattg ctgaaaagct tgaaaaagat ataccttttt attttttaag
    20281 gtatagggtt caaccgggat taagcggatg ggcacagatt aatataccac cttcaacctc
    20341 aatacaagag gcaaaagaaa aatttcaata tgatctttat tatcttaaac accgttcatt
    20401 tctatttgat ttggtaatat ttttaaagag tttaagaaaa atatttggct agttttatta
    20461 ttaagcactt ttatttttta ttcgtgttaa gcaatttaac gaggcaagtg aacagaactt
    20521 ttgttttgca atatttttgc aaataaacga ttttttaaaa agtattgttc cattttgcat
    20581 tgcattgcaa ttcttgcaaa tcttttttaa atagtttaaa ataacaaaaa ttaaaaaaaa
    20641 taataaaacc ccgcagggat tttaaataag ccccctacgg ggcttaaaat tatctcaaca
    20701 ctatgtcaaa gaaaacaacc attattctta taactatcat tgctctaatc tttattagcg
    20761 gaggagtgta tttatggtgg gaaaacaatg aaaaaacacc aagaacttat tcag
//
LOCUS       DAOTQD010000004        20323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_2701_length_20323_cov_7.534981, whole genome shotgun sequence.
ACCESSION   DAOTQD010000004 DAOTQD010000000
VERSION     DAOTQD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20323
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2701_length_20323_cov_7.534981"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /gene="rplS"
                     /locus_tag="P5570_00315"
     CDS             complement(<1..357)
                     /gene="rplS"
                     /locus_tag="P5570_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRR39199.1"
                     /translation="MEKSSKEKNKNISKKELPKMKPGDTVRVHEKILDEKKKKSQVFE
                     GILISQKHGNGINATFTLRAIVEGVGVEKTYPLHSPLIEKIEIIKKGKGRRAKLYFLR
                     TKSQKVIGKKLKSEVVK"
     gene            complement(454..526)
                     /locus_tag="P5570_00320"
     tRNA            complement(454..526)
                     /locus_tag="P5570_00320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Val,seq:gac)
     gene            complement(650..721)
                     /locus_tag="P5570_00325"
     tRNA            complement(650..721)
                     /locus_tag="P5570_00325"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(687..689),aa:Ile,seq:tat)
     gene            complement(742..3093)
                     /locus_tag="P5570_00330"
     CDS             complement(742..3093)
                     /locus_tag="P5570_00330"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HRR39200.1"
                     /translation="MVLAASVTGDTPVLVKQDGEIKLSPISKVIDACYKEDEEGIEKL
                     TPGLEVLGFEKKPAKGGFKNNLYFSNSAFKKVKSVFRHKVNEIYKIEFRGGEIKTTGN
                     HSVFVRTSEGLKTKAVANLKPGDILVNLPYKVNRTNLKKEIRAHKFNPEFNLELPLWQ
                     PLFEKFESVNFVYQYALSNTGEVSQTQLGKTFGFSQRTIGKWQQGICGPRVLSKNYYR
                     DKDVLPEKVKVTPNLMRLFGYYTAEGYARKEIDFCFNRKEKDKIEDVKRLMKTIFNLE
                     PDRVRFITPGAVNIIYYSKPVANFFSFHCGKGAKNKHLPSFIFEAPFEYFREFLRGYL
                     VGDGYIDKRGKGEITSVSKQLILELNWLFRMHGFKSYIHSFKTKKERRINNGKPLKES
                     TAWRLGFGKSQNPLNSPDSRMNGSVWRSVVKKINKIPYEGYVYDFCGCENEAFFGGES
                     PILLHNTNRPDILDPALLRPGRFDRRIVLDEPDVRGREEVLKIHSRGKPLAKDVNLRE
                     IAERTPGFSGADLANLMNEAAILAARRNKQEITQDELRESIEKVLLGPERKSHILSEK
                     EKKIAAYHEAGHALVSSFMPHSEPVQKISIVSRGMAAGYTLKLPSEERHLKTKSQFLA
                     DLATLMGGFVAERLIFKDVTTGASNDLKKATELARSLVMEYGMSRLGPMTFGKRESMV
                     FLGKEITEQRDFSEEVAEKIDKEVERFIKEAEKNAEKILKEKKDLLEKVAKALMEKET
                     IEKKEFEELIKETKAEEKKPNEDNKKSSARNNNKNIERKNLKE"
     gene            complement(3076..3660)
                     /locus_tag="P5570_00335"
                     /pseudo
     CDS             complement(3076..3660)
                     /locus_tag="P5570_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(3835..3999)
                     /locus_tag="P5570_00340"
                     /pseudo
     CDS             complement(3835..3999)
                     /locus_tag="P5570_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018218.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
     gene            complement(4008..4700)
                     /gene="pyrH"
                     /locus_tag="P5570_00345"
     CDS             complement(4008..4700)
                     /gene="pyrH"
                     /locus_tag="P5570_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRR39201.1"
                     /translation="MYTVISLGGSVIVPHLSDEGGINVSFLKQFRSLILKELKKDKKF
                     IIVAGGGKTARVYQKAAAEIVNLKKEDIDWIGIHSTRLNAHLLRTIFWKEAYPVVLDD
                     PHKKLVKGKKDLLIGSGWRPGWSTDYVTVLLAKRFKAKEIINAGDIPFVYTKDPKKFR
                     DAKPIENISWSDYQKIIPSKWMPGMSLPFDPVATRLAKELKLKVKIIKAIDLKNLKNT
                     IEGKDFKGTMIT"
     gene            complement(4764..5321)
                     /locus_tag="P5570_00350"
     CDS             complement(4764..5321)
                     /locus_tag="P5570_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRR39202.1"
                     /translation="MQKEKNYFQEKYKDFLKIPKEGDFVKGKVIQKGKGGIVLELGNF
                     KMGIIKKDDLIRSGQTPSGIKIGEEITAKIVNLENDEGFAELSLREATEDLNWKKLEE
                     LNKKGEKLSLKVYGANKGGLIFNVSGLQGFLPASQLSAKHYPKLENPSPDKVFEKLKE
                     LIGEEIEVKIITLDPKKARLILSER"
     gene            5479..6111
                     /locus_tag="P5570_00355"
     CDS             5479..6111
                     /locus_tag="P5570_00355"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRR39203.1"
                     /translation="MQIIRLNPQKINQDKLNSAIDSLIKGEVILFPTDTVYGFLANAT
                     NKKAVEKIFKIKKRPAQKPLSLFVKDIKIVKKIAKINPQQEKFLKIIWPTSPTFKKTE
                     TGKGKITAILERRKTKEKIYGIREDTIAIRIPSYKPLNLLLNQVNFPLAQTSANISGK
                     PASGKIKEIIKEFKDKKDQPDLIINAGDLPSHKPSTIIDLTTLPPKILRE"
     gene            complement(6100..6912)
                     /locus_tag="P5570_00360"
     CDS             complement(6100..6912)
                     /locus_tag="P5570_00360"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRR39204.1"
                     /translation="MKNQIILAILILTFAIFGGLFFVYQEKSIQEIQETQEMEKVSQP
                     SEEVFQLEETSQNLSSETIEIITSPPQESLPKKFKLDVPFLSQAPLEIWDDDHNEACE
                     EAAILMIDIYLKEKELIPEMVDKEILEMIDYQKENWQGHFDLSVEKIEKLAEEFYGYK
                     NTKIKYDVSIDDIKREIVSGNPVILPTAGRMLFGPLSEGKNPYYRSPGPLYHVLVAIG
                     WDDDKGVMVVNDPGTKHGKEFSFKYEVLENAIHDWNNGNVNNGKKAMVVLFP"
     gene            complement(6917..7696)
                     /locus_tag="P5570_00365"
     CDS             complement(6917..7696)
                     /locus_tag="P5570_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR39205.1"
                     /translation="MKVKKSNNLQTIYVMWLREMKRFLRAKSRVIGTLAMPFFFLFTM
                     GFGFNSGFTLPGAGPDINYLDFLVPGIIGMTMLFTSMTAGISVLWDKEFGFLKEIMVA
                     PVSRVAIVLGRIAGGVTTALIQGILILVIALFMGFKIANVGSFLLAVLFMLLIGIGFI
                     GFGVAIASMMEDTQGFSLIMNFVIFPIFLLSGTFFPITNFPNWLRPLSYIDPLTYGVD
                     GLRNSLINFSQFSAGLDFGVLAVFSIVMVILGTYLFNKTEV"
     gene            complement(7701..8690)
                     /locus_tag="P5570_00370"
     CDS             complement(7701..8690)
                     /locus_tag="P5570_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR39206.1"
                     /translation="MSIIEVKNLTKKFNGFTAVDHISFNVEEGEIFGLLGPNGAGKTT
                     TIKMLTTLLRPTEGKATVCGYDILKQRDEVRSSIGIVFQEPALDNRLTGRENLDFHAR
                     LYGLDKKTRQKRIEEVLEMVELKDKADVIVRDYSGGMQRRLEIARGLMHYPRVLFLDE
                     PTLGLDAQTRRHIWEYILSLNQREKTTIILTTHYMEEADYLCQRMAIVDFGKIVALDT
                     PANLKNILGGDVVSIEAVPSEKALPVLQKIPWAKKVTQHNGVIDLNCEEGEKKIPLLM
                     RIDEKEKEFEIKSINLRKPTLEDVFLHFTGKTIREVEGGNGEKKRMKRRTRKN"
     gene            complement(8694..8957)
                     /locus_tag="P5570_00375"
     CDS             complement(8694..8957)
                     /locus_tag="P5570_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRR39207.1"
                     /translation="MIKNKRKKNGEQFYGTATIGEKGQIVIPSEARKAMDLKKGEKLL
                     VFGMGSEMITFTKLSKMERFVSHLTDHLNTIRAVLKKAESSKK"
     gene            complement(9473..9940)
                     /locus_tag="P5570_00380"
     CDS             complement(9473..9940)
                     /locus_tag="P5570_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRR39208.1"
                     /translation="MNKYYLTNDGFKNCKREYEELKKILKEKKELKARKLEDELWRPE
                     DLNSEFEVLQTEIDFIEEKIRRIEEALKNAKIIKNSSSGSQIVNVGRTVVIENNGKIG
                     EFTIVETIEADPSNGKISNESPLGRTLLGKGIGETVTVETPSLSNTYKIINIK"
     gene            complement(10177..12567)
                     /gene="ppsA"
                     /locus_tag="P5570_00385"
     CDS             complement(10177..12567)
                     /gene="ppsA"
                     /locus_tag="P5570_00385"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HRR39209.1"
                     /translation="MRSILWFKDISIEDVPLVGGKNASLGEMIRELKEEGINVPDGFA
                     LTADAYFDFLEKGRIKDKIAKIVASIDVKNIKDLQKKSKQIQNLILKTPFPKELEEKV
                     LDFYHKLSAEYKEKETDVAVRSSATAEDLPTASFAGAHETYLNVRGEKDLLFAIKKSF
                     ASLFLPRAIFYRQEKGFDHLQIGLSSGVQKMVRSDLGSSGVIFTIDTESGFEDVILIN
                     GSWGLGEMVVKGRVITDEFIVAKRMLIEGYPAIIKKELGTKKVKMIYGTAKEKNPTKI
                     VKTTAAEQNQFTLRDEEVLKLADWAVKVERHYSKKAGEPRPMDIEWAKDGKTGQLFFV
                     QARPETVHRAQEKNIFTEYYLQGKGRIITKGIAVGKKIAQGKANVIKDIKEIDKFKKG
                     EVLVTRMTDPDWEQIMKIASAIVTDEGSRVCHAAIVSRELGIPCIVGTKKGTQVISKG
                     KDITIDCSKGEEGYVLEGKIPFKKIDHQLKEIPRTKTKVCLNIGSPDIAFRSSFLPND
                     GVGLAREEFIFASEIKIHPLALLNPKKLDKKTQKEIERITAPWSSKKEFFIEKLAQGV
                     GRIATAFYPKQVIVRFSDFKSNEYAQLLGGQIFEPKEENPMLGWRGASRYYSEEFKEA
                     FLLECEAIKKAREEYGFRNIAVMIPFCRTVKEGKETLELMQNAGLVKGKNGLKVYVMC
                     EIPSNVILAEEFLKVFDGFSIGSNDLTQLTLGLDRDSAIVAKVGDERDEAVKRLVKEA
                     IKVCREKEKYSGICGQAPSDYPEFTEFLIDCGIESISVNPDVAIKTRLIIAEKERT"
     assembly_gap    12573..12672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12693..13010)
                     /gene="rpsP"
                     /locus_tag="P5570_00390"
     CDS             complement(12693..13010)
                     /gene="rpsP"
                     /locus_tag="P5570_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRR39210.1"
                     /translation="MLIIRLQRVGKKNQPMFRVVLTEKTAPPKGRYIEKLGYLDPVTK
                     KNSFNTERINYWISKGAQPSDTVWNLLVKNEIIKEKKRPVKIKTKKEKKATKVEKPEA
                     ETK"
     gene            complement(13083..13835)
                     /gene="rnc"
                     /locus_tag="P5570_00395"
     CDS             complement(13083..13835)
                     /gene="rnc"
                     /locus_tag="P5570_00395"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRR39211.1"
                     /translation="MTTTSDNDNLEKLEEVLGVHFKNKNLLRQALIHRSYLNEHPKLP
                     LQHNERLEFLGDAVLELAVSEHLYKNLNKPEGEMTNLRAALVNTNNLSGIAKKLGIED
                     YLWLSKGEKKDTGRAREVLLANALEAIIGATYLDKGFEAARDFIKKNILIPCLPKLLQ
                     KKVIKDAKSYFQEIVQEKIGVTPSYQVIKEWGPDHKKQFEVGVYLDKKLIATGQGFSI
                     KEAEEKAAEKAIEDLNPKSQITNNKSQITNKS"
     gene            complement(13813..14256)
                     /gene="nusB"
                     /locus_tag="P5570_00400"
     CDS             complement(13813..14256)
                     /gene="nusB"
                     /locus_tag="P5570_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRR39212.1"
                     /translation="MSYRHLSRSIAMQSLYEWDFRQLPKKELSFIVERNINDFGPGME
                     ETDFTRKLISGIIENLLKIDKIIEASAPQWPVPQINIIDRNILRIGIYELLFGEKEEV
                     PPKVAINEAIELAKNFSGQASAKFVNGVLGTIYKEMNINDNDKRQ"
     gene            complement(14453..14617)
                     /gene="rpmF"
                     /locus_tag="P5570_00405"
                     /pseudo
     CDS             complement(14453..14617)
                     /gene="rpmF"
                     /locus_tag="P5570_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511468.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
     gene            complement(14735..15613)
                     /gene="secF"
                     /locus_tag="P5570_00410"
     CDS             complement(14735..15613)
                     /gene="secF"
                     /locus_tag="P5570_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRR39213.1"
                     /translation="MDFIRYSNIYFIFSGILILASIAAFFTVGLKPGIDFIGGNLLEV
                     EFKNQPPLVEELRKDLSGIIKGDFTLQETGENGIVLRAKEISDQEHQEILEIFKTKGG
                     DFEEKKFEMISSVVGNEFKNNTIIAIILSCIAIMIYIALAFRHASRLISSWQYSVAAV
                     IALLHNVLITCGVFVFLGKFINLEINIPFVAALLTVIGYSINDTIVIFDRCRENLNRG
                     GDDLQKILNRSLTETIARSIFVSFTTLLTLFAILFWGGETLFPFVLTLIVGIATGTYS
                     SIFIATPLLYKFTNRK"
     gene            complement(15622..16998)
                     /gene="secD"
                     /locus_tag="P5570_00415"
     CDS             complement(15622..16998)
                     /gene="secD"
                     /locus_tag="P5570_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRR39214.1"
                     /translation="MNKQTKNWIFVVIVLVLAIFLALFAMPGRIAERLNISVPEFMQK
                     PYKLGLDLQGGVHLLYQADLSQVSKEDWQNSMEGLRDVIERRVNLFGVQEPIVQVQKS
                     GDEYRLSVELAGITDPDEAIEMIGKTPYLEFREEVTEEEKEEILNELRKEIGPKANEL
                     VDQPFFKPTELTGKYLKGAKLEFDSTTYKPQISLEFNDEGAKLFEEITERNVGKILAI
                     YIDNQLIQAPTVQEKISGGKAQITGNFTIEEARGLVQNLNAGALPVPIELISQQSIGP
                     SLGKESIDKSVKAGMIGFGLVILFMILIYRFGGFLASIALMIYAVMLLSLFKVIPVTL
                     TLSGIAGFILSVGMAVDANILVFERLREEIKFKNQLKDKRNQLQQTDIDNAFSRAWLA
                     IRDGNFTTLIICAILFLIASGFVQGFSVTLSLGILISMFTVMIITKLFIKTFSQTGLK
                     NFPKIWTR"
     gene            complement(17085..18785)
                     /locus_tag="P5570_00420"
     CDS             complement(17085..18785)
                     /locus_tag="P5570_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRR39215.1"
                     /translation="MNSQNSHLYVLMFLFFSLGATIIGRLFYIQVNQYNHYKELADLQ
                     QIDEEDLKAERGEIFFSDESTVLATNIGTKKVFIVPREIKNIEKTSEILADVLKMEKA
                     DVFEKASQIDDPWIFLKEIPAIEGEKIKNIAGVHFESYLERFYPRERMASHVLGFYNK
                     ENKGQYGIEQYWQEELRGVDGYRKGVKDAKGKFILPLTKIQEPVDGNDLILTLDPNIQ
                     LFLEIKTKEIFEKYAPRNATIIVLAPKTGEVLGMASLPNFDPNNYEEKENISVFKNPA
                     ILAYEPGSIFKPFTAAMAIEEKKVTPQTTYYDSGEIKIGSHTIRNSDLKSHGTQTMTN
                     VIELSLNTGAVFMQQQVGNDKFLDYVKKFGFGTKTGIDLSGEETGSIANILHPQSGDG
                     AIEYANASFGQGISATPIQILTAFTAIANQGQMVKPHLVKKIIHSSDGTEEEIETEVI
                     GQVISAETASRVTAMMVSAVKNGYGKKAEVEGYLIAGKTGTAQASWSYLGENKGGYSS
                     FFIQSFVDFAPAFDPAFIFILKMDAPTKGPQFSSDSLGPFAQEINEYLFNYLGIPPEE
                     "
     gene            complement(18798..19202)
                     /locus_tag="P5570_00425"
     CDS             complement(18798..19202)
                     /locus_tag="P5570_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39216.1"
                     /translation="MPQALYTYTISKTSLSGKRKDVAKNIKTSRGSRINLSMVLSIIF
                     LLLFIACFCLYIYFTIQMVSINFDLRAKENKLLVLQDENKILETEIKEGLSLEAMEEK
                     VQSLELVRAGDIRYLQFSTSSSLSQASPKEKR"
     gene            complement(19234..20133)
                     /gene="rsmH"
                     /locus_tag="P5570_00430"
     CDS             complement(19234..20133)
                     /gene="rsmH"
                     /locus_tag="P5570_00430"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRR39217.1"
                     /translation="MVHVPVLVEEVLYYLDPRPNDNFIDCTVGEGGHTIAILEKNAPN
                     GKVLAIDADPEQIKNYKLTKRDSDNRVILVNDNFKNLKKIVENFCPDWQGKIRGILFD
                     LGMSSWHLEKSKRGFSFRYNEDLDMCYGKTATVSAKEIINQWPSQEIERIIKEYGEER
                     YAWRIADKIAKQRKQKPILTTFDLIEVIREAVPKNYERGRIHPATRTFQALRIVVNQE
                     LENLKVALPQALEILSPGGRIVVISFHSLEDRIVKNFFKEKAKEGKIKILTKKPVIPS
                     QEEIEKNKKSHSAKLRAAVKLTF"
BASE COUNT         6366 a         3983 c         2571 g         7303 t
ORIGIN      
        1 ttttactacc tcagatttta atttttttcc tataactttt tgcgattttg tccttaaaaa
       61 ataaagcttt gctcttcttc cttttccctt ttttattatt tcaatttttt caatcaacgg
      121 agagtgtaaa ggatatgttt tttccactcc tactccctca actattgctc ttaaagtaaa
      181 agtagcattg atcccattgc catgtttttg agaaatcaaa attccctcaa atacttgaga
      241 ttttttcttt ttttcatcaa gaattttctc atgaacacgc actgtatctc caggtttcat
      301 ctttggtaat tcctttttag atatattttt atttttttct ttagatgatt tttccataat
      361 ttttttattt ttattaatat atttttcgac caaacttaga ataaactatt tattattttt
      421 aatactatta atcgaaaact agaaccaaaa ctgtgggcga tataggactc gaacctataa
      481 cctttcgcac gtcaagcgaa cgctctagcc attgagctaa tcgcccctaa ttttaatctc
      541 ctattactcc tttacataat ccaaatctta atacctaaaa aacctccaac catctcttgc
      601 caaaaacttt ttgaaatttt tggttctttt tcttcggcca tatatggatt gcgcacggca
      661 gggatcgaac ctgcgacttc tactttataa gagtagcgtt ctaccactga actacgtgcg
      721 caaataaaaa ccaagagcta attattcttt taaatttttt ctttctatgt ttttattgtt
      781 atttctagcg ctactttttt tgttgtcctc gtttggtttt ttttcttctg cttttgtttc
      841 ttttattaat tcttcaaatt cctttttttc aattgtttct ttttccataa gagctttagc
      901 gactttttca agcaaatctt tcttttcttt taaaattttc tcagcatttt tttctgcctc
      961 ttttataaat ctttctactt ctttgtcaat cttttctgcc acttcttcag aaaaatctct
     1021 ttgctcagta atttccttac ctaaaaacac catactttct cttttgccaa aagtcattgg
     1081 tcccagccgc gacattccat attccattac taaacttcgg gccaattcag ttgctttttt
     1141 aagatcattt gaagcaccag tagttacatc tttaaaaatc aatctttcag caacaaaacc
     1201 acccattaag gttgctaaat ctgctaaaaa ctgtgatttt gtttttaaat gcctttcttc
     1261 acttggcaat tttaaagtat atccagcagc cattcctcgg gaaacaatcg aaattttttg
     1321 aactggctct gaatgcggca taaaagaaga taccaaagcg tgccctgctt catgatatgc
     1381 agcgattttc ttttcttttt ctgataaaat atgacttttc ctttccggac ccaaaagcac
     1441 tttttcaatt gattcacgaa gttcatcttg agtaatctct tgtttgttac gtctggctgc
     1501 taaaattgcc gcttcgttca ttaaattagc taaatctgct ccagaaaatc caggcgttct
     1561 ttcagcgatc tcccttaaat taacgtcttt tgccaatggt tttccacgag aatgaatttt
     1621 taaaacttct tctcttcccc taacgtctgg ttcatctaaa actattcttc tatcaaacct
     1681 tcccggtctt aataaagcag gatctaagat atccggacgg ttggtattgt gcaaaagtat
     1741 aggactttct cctccaaaaa aagcttcatt ttcgcaacca caaaaatcat aaacatatcc
     1801 ctcataagga attttattta tttttttaac taccgaacgc caaacagagc cattcattct
     1861 tgaatcaggt gagtttaagg gattttgact tttcccaaat cccaatctcc acgcggtact
     1921 ttcttttaag ggcttaccat tattaattcg cctttctttt ttagttttaa atgagtgaat
     1981 atagctcttg aatccgtgca ttctgaaaag ccaatttaat tctaaaatta attgtttgct
     2041 tacagaagta atctctcctt tccctctttt atctatataa ccatctccga ctaaataccc
     2101 tcttaaaaat tctctaaaat attcaaaagg tgcctcaaaa ataaaggaag gtaaatgttt
     2161 atttttagct cccttgccac aatgaaagga aaagaagtta gcaacaggtt tagaataata
     2221 aataatatta acagcaccag gagtaataaa tcttactcta tcgggttcta aattaaagat
     2281 tgtcttcatc aatcttttta catcctcaat cttatctttt tctttcctat taaaacaaaa
     2341 atcaatctct tttctagcat aaccttcggc tgtgtaatac ccaaaaagcc gcattaaatt
     2401 aggcgtcacc ttcaccttct ccggtaaaac atccttgtct cgataataat tttttgaaag
     2461 tactctcggg ccgcatattc cttgttgcca tttaccaatt gttctttgag aaaaaccgaa
     2521 tgttttacct aattgagttt gagaaacttc tccggtattg gatagggcat attgataaac
     2581 aaaattaacc gattcaaatt tttcaaataa aggttgccaa agcggaagtt caagattaaa
     2641 ttccgggtta aatttatgag ctctgatttc tttttttaaa tttgtcctat taactttata
     2701 aggaaggttt actaaaatat cgcctggttt taaattagca actgcctttg tttttagtcc
     2761 ttctgatgtt cgcacaaaaa cagaatgatt gccggttgtt ttaatctctc cacctctaaa
     2821 ctcgatctta tagatttcgt ttactttatg tctaaaaaca ctttttactt ttttaaaagc
     2881 actatttgaa aaatataaat tattcttaaa gcctccctta gcaggttttt tctcaaatcc
     2941 taacacctca aggcctggcg ttaatttttc aataccttct tcatcctcct tataacaagc
     3001 atcgatcact ttagagattg gggaaagttt aatttcccca tcttgtttta ccaaaacagg
     3061 agtatcgcca gttacgcttg ctgctagaac aataacatta gtttctcttt caaaaccatc
     3121 catttcaacc aaaatctgat ttagggtttg ttctctttcg tcgtgtcccc caccaagacc
     3181 agcaccacga tgcctaccaa tagcatccag ttcatcaata aaaacaatgc aaggtgcatt
     3241 tttcttggca gtagtaaata aatctcttac tcgagcgctt ccaacgccaa caaacatctc
     3301 aacaaactct gatccagata ttgaaaagaa aggaactccg gcttctgaag ctacagccct
     3361 ggcaagcaat gtttttcctg ttccaggcgc accaaccaat aaaacccctc taggaattct
     3421 tgcacccata ttaagaaatt tttgagggtt ttttaaaaaa tcaactactt cttttaattc
     3481 ttcttttgct tcttgaagat cagccacatc atcaaatgta accttttctt ttatttttcc
     3541 tccaccgcca tatacctttg gctgagcttt gccaaaagaa agagcttggg tggctccttg
     3601 ctgcccctgt cttaacatcc accaaaataa aaaaccaata aggataattg gcaaaatcga
     3661 aagcaaaacc cataaccaaa tatcactact ttcggcctga ggaacaatac ttacacttct
     3721 taataatgtt ggatcaagcc cataaccgtt taccaaaatt tcttccagtg gtgtgcctat
     3781 ttcttttgta gcaatctctt tagcgccatc atctaattca atatttatct gtgtcccctg
     3841 aacctcaatt ttttttacct gaccattttc aatttgctga acaagttcac caagagtaac
     3901 ttcttccact ttttgaggag gattaaataa agaaaaaaag gacgctaaaa atagaaaaat
     3961 taaaaatgta tataaaatat tcttccaaat gtttttcatg ttttatttta cgttatcatt
     4021 gttcctttaa aatcttttcc ctcaatagta ttttttaaat ttttaagatc tatagctttg
     4081 ataattttta cttttaattt taattctttt gctaacctag ttgcgacagg atcaaaaggt
     4141 aacgacatac caggcatcca ctttgaggga ataatctttt gataatcact ccaagaaata
     4201 ttttcaatgg gttttgcatc tctaaatttt ttaggatctt ttgtataaac aaagggaata
     4261 tccccagcat taataatttc tttggcttta aatctttttg ctaaaagcac tgttacataa
     4321 tcagtagacc aacctggccg ccatccagaa ccaattaaaa gatctttttt accttttacc
     4381 aattttttat gaggatcatc taatactaca ggataggcct ctttccaaaa aattgttcgc
     4441 aaaagatgag catttaacct agtagaatga ataccgatcc aatcaatgtc ttctttttta
     4501 agattgacaa tttcagccgc ggctttttga taaaccctag ctgtttttcc accgccagct
     4561 acaataataa attttttatc tttttttaat tcttttaaga ttaaacttct aaattgtttt
     4621 aaaaaagaaa cattaatacc tccctcatca gaaagatgag gaacaattac agaaccgcca
     4681 agagaaataa ctgtatacat aaatgactaa aactattgtt ttattgaact actttttgat
     4741 ttttaatatt taattttatc agattatctt tctgataaaa ttaatcttgc ttttttagga
     4801 tcgagagtaa taatttttac ctcaatttct tctcctatta attctttcaa tttttcaaat
     4861 accttatcag gggaaggatt ttctaatttt ggataatgtt ttgctgacag ttgtgaagca
     4921 ggcaaaaacc cttgcaaacc agaaacatta aagatcaacc ctcctttgtt ggctccatat
     4981 acttttaagg ataatttttc tcctttttta tttaactctt ctaatttttt ccaattcagg
     5041 tcttctgtag cttccctcaa agacaattcc gcaaatcctt catcgttttc aagatttaca
     5101 atttttgctg ttatttcttc tccaatttta attcctgaag gagtttgacc agatcgaatt
     5161 aaatcatctt ttttgataat acccatttta aaattcccta attccaaaac aatacctcct
     5221 tttccttttt gaattacttt tcccttaacg aaatctcctt cctttggaat ttttaaaaaa
     5281 tctttatatt tttcttgaaa ataatttttt tctttttgca taagtataat tccaatctat
     5341 gaataatatt ccaacaatca gaatattatt caggatgttt tttgaataaa acctacaaag
     5401 caggttatat taattataca aattttaata aaatttgcaa ataatcaaaa cgaatattat
     5461 aattattatt aaaaaaatat gcagattata agattaaatc ctcaaaaaat caaccaagat
     5521 aaattaaatt ctgcaataga ttctctaatt aagggagagg ttattctttt tcctactgat
     5581 acagtttatg gatttttagc taacgcaaca aacaaaaaag cagtagaaaa aatcttcaaa
     5641 ataaaaaaaa ggccagctca aaaacccctt tctcttttcg ttaaagacat taaaatagta
     5701 aaaaaaattg caaagattaa tccccaacaa gaaaaatttt taaaaattat ttggcctacc
     5761 agtccaactt tcaaaaaaac cgaaacaggc aagggtaaaa taacagcaat acttgaaaga
     5821 agaaagacaa aagaaaaaat ttatggcata agggaggaca ctattgccat tcgtatcccc
     5881 tcttataaac cccttaatct tttgttaaac caagttaatt ttcccctagc ccaaacatca
     5941 gccaatattt ctgggaaacc cgcttcggga aaaataaaag agattatcaa agaatttaaa
     6001 gacaagaaag atcaacctga tttgatcatt aatgccggtg atcttccctc tcacaaacca
     6061 tctacaatta ttgatttaac aactcttccg ccaaaaattt taagggaata aaacgaccat
     6121 tgcttttttt ccattgttaa catttccatt attccaatca tgaatagcat tttctaaaac
     6181 ctcgtattta aaagaaaact ctttaccgtg ttttgttcct gggtcattaa caaccattac
     6241 tcctttatca tcgtcccagc caatcgctac taacacatga taaagaggac cgggcgagcg
     6301 ataataagga tttttaccct cggaaagtgg tccgaaaagc attctaccgg ctgtcggcag
     6361 aattaccggg ttaccactca caatttctct tttaatatca tcaattgaaa catcatattt
     6421 tatctttgta tttttataac cgtaaaattc ctctgctagt ttttctatct tttcaactga
     6481 taaatcaaaa tgaccttgcc aattttcttt ttgataatca atcatttcta aaatctcctt
     6541 atcaaccatt tctggtatta attctttttc ttttagataa atatctatca ttaaaattgc
     6601 cgcttcctca catgcttcgt tgtgatcatc atcccaaatt tccaaaggcg cttgcgacaa
     6661 aaaaggcaca tctagtttaa attttttggg caaagattct tggggaggag aggttattat
     6721 ttctattgtt tctgaagata aattttgaga agtttcttct agctgaaaaa cttcttctga
     6781 tggttgagaa actttttcca tctcttgagt ctcttgaatc tcttgaatac ttttttcttg
     6841 ataaacaaaa aataaaccgc caaaaatagc aaaagttaaa attaaaattg ctaaaataat
     6901 ttgatttttc attgtattac acttctgttt tattaaataa ataagtacct aaaataacca
     6961 ttactatgct aaaaacagcg agaacaccaa agtccaaacc tgcagaaaat tgagaaaaat
     7021 taatcaaact gttacgcaat ccatctactc cataggtcaa aggatcaata taggataacg
     7081 gtcttaacca attaggaaaa ttcgttattg gaaagaaagt accagacaaa agaaaaattg
     7141 gaaaaatcac aaagttcata attagagaaa atccttgagt atcctccatc attgaagcaa
     7201 ttgccactcc aaatccaata aaaccgatgc caataagaag cataaataat actgccagca
     7261 aaaaacttcc cacattggct attttaaatc ccataaacaa tgcaataacc aaaatcaaaa
     7321 ttccttgaat taaagccgta gtaactcctc cagcaattct tccaagaaca atagcaactc
     7381 gactgaccgg tgcgaccatt atttctttta aaaaaccaaa ttctttatcc catagaaccg
     7441 aaatgccagc agtcattgaa gtaaaaagca ttgtcatacc aataataccc ggaaccaaaa
     7501 aatcaagata atttatatct ggcccagcac ctggtagagt aaaaccagaa ttaaacccaa
     7561 atcccattgt aaaaagaaaa aagaatggca ttgccaaggt gccaattacc cgactttttg
     7621 ctcttaaaaa tcttttcatt tctcttaacc acattacata aattgtttgc aagttgtttg
     7681 atttttttac tttcatattt ttaatttttt cttgttcgtc ttttcattct ttttttctcg
     7741 ccattacccc cttctacctc tctaatggtt ttgcctgtaa agtgcaaaaa gacatcttct
     7801 aaggttggct ttcttaaatt aattgattta atttcgaatt ccttttcttt ttcgtctatt
     7861 ctcattaaaa gaggaatttt cttttctcct tcttcacaat ttaaatcaat cacgccatta
     7921 tgctgagtaa ccttttttgc ccaaggaatt ttttgaagca cgggcaaggc cttctcagaa
     7981 ggtactgctt caattgaaac aacgtctcct cctaaaatat tttttaaatt ggcgggtgta
     8041 tccagggcta caatctttcc aaaatcaaca atcgccatcc gctgacatag ataatcagct
     8101 tcttccatat aatgagttgt taaaataatt gttgttttct ctctttgatt taagcttaat
     8161 atatactccc aaatatgacg tcgtgtttga gcatcaagac ctaaagttgg ctcatctaaa
     8221 aataaaactc tgggatagtg cataagaccc ctggcaattt ccaatcttct ttgcattcca
     8281 ccagaataat ctcttactat cacatcagct ttgtctttta attcaaccat ttctaacacc
     8341 tcttcaatcc ttttttgccg agttttttta tcaagaccat aaagacgagc gtgaaaatct
     8401 aaattttctc taccagttaa acgattatct aaggccggct cttgaaaaac aataccaata
     8461 gaacttctta cttcatctcg ctgttttaaa atatcatatc cacaaactgt tgctttccct
     8521 tctgtaggtc taagtaaagt tgttagcatt tttatggttg ttgttttccc agcgccatta
     8581 ggacccaata aaccaaaaat ctctccttcc tcaacattaa aggaaatatg atcaacggca
     8641 gtaaatccat taaatttttt ggttaaattt tttacttcaa ttattgacat atattatttt
     8701 tttgaagatt ctgctttttt aagaacagca cgtattgtgt ttaaatgatc tgttagatga
     8761 gaaacaaacc tttccatctt tgataatttt gtaaaggtaa tcatttcact ccccattcca
     8821 aaaaccaaaa gtttttctcc ttttttaaga tccattgctt ttcttgcttc gctgggaata
     8881 acaatttgcc ccttttcacc aatagtggct gttccataaa actgttcgcc gttctttttt
     8941 cttttgtttt ttatcataaa aacatcttca agataaaatt aaatatattt tcctacttgt
     9001 aggaaaagta tgattttatc aacaatttaa attatactct tgtttttttt aatgtcaaga
     9061 tgtttaaaaa tagttgagga ttaaaaagta aataatccag tataactaat atttataaaa
     9121 tcaaattcca aaattcaaaa acgttttttg agtttaaaat tttaggcctg gggatttaat
     9181 ttaaaatgaa atttatggaa gcaaaaagcc ttggaaatcc ccaaggcctt ctgccgactt
     9241 tagttggagt cccttgatct ctctcaggac tgcactcaca ttgttatttt aacaaaaaaa
     9301 aacaaaaaag tcaaattttt atatgatgta ctccacttag ttagatatag attatatgga
     9361 catataatac ctaatacagc cagtctagat tactggccag taaggacaaa tatcacaaat
     9421 aaagccaaaa aaatttcaaa agaatttaag atattctttt taaatcttct cattatttaa
     9481 tgtttattat tttataggtg ttgcttaaag atggcgtctc aacagtaaca gtttccccta
     9541 tgcctttccc cagtaaagtc cttcctagtg gcgattcatt ggaaatctta ccattactag
     9601 gatctgcttc aatggtttcc acaatggtaa actcaccaat tttaccattg ttttcaataa
     9661 ctactgttct tcccacattt actatctgag atcctgatga agaatttttt ataattttag
     9721 cattttttaa tgcttcttct attcttctta tcttttcttc aataaaatca atttcggttt
     9781 gaagtacttc aaactcagaa tttaaatctt ctggcctcca taattcatct tccaatttcc
     9841 ttgcttttaa ttctttcttt tcttttaata tcttttttaa ttcttcgtat tctctcttac
     9901 aatttttgaa accgtcatta gttaaataat atttattcat aattttaaaa ttaaaaaacc
     9961 cctttggtga aggggttagt gatttttaaa tttttgataa ttttaaaaaa tcaaattcct
    10021 atccccgtca ccatagggct taagcaaaca atcaattttt tagaaaacat cttaatcaac
    10081 gtttccataa attagtttaa gtgaattaat aaaaaattac tttttatgtt ttgcaatcaa
    10141 tctagtaatt atagtttaat ctttttttaa aaattgtcaa gttctttctt tttcagcaat
    10201 aattaatctg gtttttattg ccacatcagg gtttacagaa atactttcaa tgccacaatc
    10261 aattaaaaat tcagtaaatt ctggataatc acttggggct tgaccacaga taccagaata
    10321 tttttccttt tctctgcaaa ccttaattgc ttctttgact aatcgtttta ctgcttcatc
    10381 tctttcatct cctacttttg caacaatagc actatctcga tccaacccca atgttaattg
    10441 ggttagatca ttagaaccaa ttgaaaaacc atcaaatacc tttaaaaatt cttctgctaa
    10501 aatcacatta gagggaattt cacacattac atatactttc aaaccatttt ttccctttac
    10561 caggcctgca ttttgcatta attcaagagt ttctttacct tctttaacag ttcgacagaa
    10621 aggtatcata actgcaatat ttcggaaacc atattcttct cttgcttttt taattgcctc
    10681 acattcaagt aaaaacgctt ctttaaattc ctctgaataa tatcttgaag cacctctcca
    10741 acccagcatc ggattttctt cttttggttc aaaaatctga cctcctaata attgagcata
    10801 ttcatttgat ttaaaatcag aaaaacgaac aatcacctgt tttggataaa aagcggttgc
    10861 aatacgaccc actccttgag ccaatttttc aataaaaaat tcttttttac tggaccaagg
    10921 agcagtaatt ctttcaattt ctttttgtgt ttttttatct aattttttgg gatttaataa
    10981 ggccaaagga tgaattttta tttctgatgc aaaaataaat tcttcccttg caagtcccac
    11041 cccgtcattt ggcagaaaag acgacctaaa ggcaatgtca ggagaaccaa tattcaaaca
    11101 tacctttgtt ttggtacgcg gaatttcttt taattgatga tcaatttttt tgaaaggaat
    11161 ttttccttcc aatacataac cttcttctcc ttttgaacag tcaatagtaa tatcttttcc
    11221 ttttgaaatc acctgagtac ctttctttgt acctacaata caagggatac ctaattcacg
    11281 agagacaata gccgcatgac atacccttga cccttcatca gtcacaatag cagaagcaat
    11341 tttcattatt tgttcccaat ctggatcagt cattcgtgtt accaatacct ctcctttctt
    11401 aaatttatca atctctttta tgtctttaat aacattggct ttgccttgag caattttctt
    11461 gccaacagca ataccctttg taataattct tcccttgcct tgaagataat attcagtgaa
    11521 aatgtttttt tcttgtgctc gatggacagt ttctggtcgt gcttgtacaa aaaataattg
    11581 tccggtctta ccgtcctttg cccattcaat atccattggc cgaggctcgc cggctttttt
    11641 agaatagtgt ctttctactt ttacagccca atctgccaat tttaacactt cctcatctct
    11701 aagcgtgaat tgattttgtt cagctgcagt agttttaaca atttttgttg gatttttttc
    11761 ctttgctgta ccataaatca tcttcacttt ttttgtgccc aattcctttt taataatagc
    11821 aggataaccc tcaattaaca ttcttttagc aacaataaat tcatcagtta taacccgtcc
    11881 ttttaccacc atttctccta gtccccaaga accatttatt aaaatcacat cttcgaaccc
    11941 actttctgtg tcaatagtaa atatgacgcc tgaagaacca agatctgacc gtaccatttt
    12001 ttgaacaccg cttgaaagac caatttgtaa atgatcaaaa cctttttctt gacgataaaa
    12061 aatggctcgt ggtaaaaaca aagaagcgaa acttttttta atagcaaata ataaatcttt
    12121 ctctcctctt acgttaagat atgtttcgtg agcgccagca aaactagctg taggaagatc
    12181 ttcggcagta gcgctggaac gaacagctac atcagtttcc ttttctttat attctgctga
    12241 taatttatga taaaaatcca ataccttttc ttctaattct ttaggaaaag gggtttttaa
    12301 aataagattt tgaatttgct tagatttttt ctggaggtct ttaatattct taacatcaat
    12361 agaggcgaca attttagcaa ttttatcttt aattcttcct ttttctaaaa agtcaaaata
    12421 agcatctgcg gttaaagcaa atccgtcggg aacattaatt ccctcttctt ttaattccct
    12481 tatcatttcc cccaatgaag catttttccc gcccaccaaa ggaacatcct caatagaaat
    12541 atctttaaac caaagtatag aacgcataaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnaattgaaa attttataga aattattttg tttccgcttc tggtttttca
    12721 acttttgtgg ctttcttttc tttttttgtt tttattttaa ctggtctttt tttctcttta
    12781 ataatctcat ttttcaccaa taaattccaa actgtatctg atggctgcgc tccttttgaa
    12841 atccaataat ttattctttc tgtattaaaa gaattttttt tggttactgg atccaaataa
    12901 cccaattttt caatatacct cccttttgga ggagctgttt tttcagtcaa aaccactcta
    12961 aacattggtt ggttcttttt acctactctt tgaagtcgaa taattaacat aaaagtttaa
    13021 attctaaatt ccaagcgcta aattcaaaat tcaaaactgt tttgaatttt agttattgga
    13081 atttaagatt tgtttgttat ttgagatttg ttatttgtga tttgggattt gggatttaaa
    13141 tcttctatgg ctttttctgc cgctttctct tctgcctcct tgatagaaaa tccttggccc
    13201 gtagcaataa gttttttgtc caaataaaca cctacttcaa attgtttttt gtgatctggc
    13261 ccccattctt taatcacttg atacgatggc gtaactccaa ttttttcctg aacaatttct
    13321 tgaaaataac tttttgcatc ctttatcact tttttttgca ataattttgg caagcaaggt
    13381 attaatatat tttttttaat aaagtcccta gcagcttcaa atcctttatc tagataagta
    13441 gcaccgataa ttgcttctag ggcattagcc aataatactt ctcttgctcg tcctgtatct
    13501 tttttttctc cttttgacaa ccataaataa tcctcaatcc ccagtttttt agcaatacca
    13561 gaaagattat ttgtatttac caatgctgct cgtaaattgg tcatctctcc ctctggtttg
    13621 ttcaagtttt tatacaaatg ctcacttacc gcaagttcta aaactgcgtc tcctaaaaac
    13681 tctaatcttt cattgtgttg taaaggaagt tttgggtgtt cgtttaaata cgaacggtgg
    13741 attagtgctt gtcttaataa atttttattt ttaaaatgaa cacccaatac ttcctctagc
    13801 ttctctaaat tatcattgtc gcttgtcgtt gtcatttata ttcatctctt tatatattgt
    13861 tcccaataca ccattgacaa attttgctga tgcctggcca gaaaagtttt tagcaagttc
    13921 tatcgcttca tttattgcta ctttaggagg tacctcttct ttctcaccaa acagtaattc
    13981 ataaatacca atcctcaaaa tgttcctatc aataatattt atctgaggta ctggccattg
    14041 gggagcagat gcttcaatta ttttatcaat ttttaataaa ttttcaataa ttcctgaaat
    14101 caattttcgg gtaaaatcag tttcctccat accaggccca aaatcattaa tatttctttc
    14161 aacaataaaa gacaattctt ttttaggcaa ctgcctgaaa tcccactcgt atagtgattg
    14221 cattgcaatt gaacgagaaa gatgacgata agacatatat ttagcttttt agaaattagc
    14281 ttattatctt tttagaaatt tctaaaaaga taaaaagcta caagctaaga agctattttt
    14341 tacttaattc ttccattgtt aagggttttt tctgctttcc ctccttttct acttgggcaa
    14401 tttctttttc tttttgttta cgttctctct tttcaagttt tgctaaaaca tcaattgcct
    14461 ccctgctatt ataataacca cagctttgac aggcgtgatg aggcaatatc ggacttttac
    14521 attttggaca caaagaaaaa cgctctggct ttaaagccag atgggatcgt cttttattcc
    14581 ttcttgattt agtatgtctt tgttttggag caggcatatt ttaaacctcg tttaatatct
    14641 tattaattat aagaataaca aattattatc aattttgcaa gtattattag aaaaaatatt
    14701 ttctgatctc atcttgttac aaacttgaat ctgtctattt tctattagta aatttataaa
    14761 gcaagggtgt ggcaataaaa attgaagaat aagttcctgt cgcaattcca acaattaagg
    14821 taagaacaaa aggaaaaagt gtttctcctc cccaaaaaag tatagcaaag agagtaagca
    14881 aagtggtaaa agagacaaat attgatcgag caattgtttc tgttaaggac ctatttaaaa
    14941 tcttttgaag atcatcccct cctctgttta aattttccct gcatcgatca aaaataacaa
    15001 tagtatcatt aatagaataa ccaataacag ttaaaagcgc agctacaaaa ggaatgttta
    15061 tttcaagatt aataaacttt cccaagaaaa caaaaacacc acaggtaatt aaaacattat
    15121 gcaaaagagc aataacagct gccacgctat attgccaaga actaattaat cttgaagcat
    15181 gacgaaaagc caaagcaata taaatcataa tagcaataca agagagaata attgcaataa
    15241 tggtattatt tttaaattca tttcccacaa ctgaactaat catttcaaat ttcttttctt
    15301 caaaatctcc tccctttgtt ttaaaaatct ctaatatttc ttgatgttcc tgatcagaaa
    15361 tttcttttgc ccttagcaca attccattct cgcctgtttc ttgtaaagta aaatcaccct
    15421 ttattatacc cgaaagatct tttctcaact cttctaccaa aggaggttga tttttaaatt
    15481 caacttctaa taaattacca ccaataaaat caataccagg tttcaacccc acagtaaaaa
    15541 aagccgcaat acttgctaaa atcaatatac cagaaaaaat aaaatatata tttgaataac
    15601 gaataaagtc cataataatg attaacgtgt ccaaattttt ggaaaatttt ttaatccggt
    15661 ttgactaaat gtttttataa aaagctttgt aataatcatt acagtaaaca tacttattaa
    15721 aatacccaaa gacaaagtta ctgaaaaacc ttgcacaaat ccagatgcaa ttaaaaacaa
    15781 aatagcgcaa ataatcaatg tggtaaaatt accatcccta attgctaacc atgccctcga
    15841 aaaagcatta tcaatatctg tttgttgtaa ttggtttctt ttatctttta gctgattttt
    15901 aaatttaatc tcttctctta atctttcaaa aactaatatg ttagcatcca ctgccattcc
    15961 aactgacaaa ataaatcctg caattccaga caaagttaat gttactggaa tcaccttaaa
    16021 caaagaaaga agcataactg cataaatcat caaagctatt gaagccaaaa aaccaccaaa
    16081 tctataaatc aaaatcataa ataaaattac taatccaaaa ccaatcattc ctgcctttac
    16141 acttttatca atcgattctt ttcccaacga cggaccaatg ctttgctgag aaatcaattc
    16201 tattggcaca ggtagggctc cagcatttaa attttgaact aatccccttg cttcttcaat
    16261 agtaaaattt cctgtaatct gagctttgcc accagaaatt ttttcttgaa cagtaggagc
    16321 ttgaattaat tgattatcaa tataaatggc taatatttta ccaacatttc tttcagtaat
    16381 ctcttcaaac agctttgctc cctcgtcatt aaactctaag gaaatttggg gcttataagt
    16441 agtagaatca aattccaatt tcgctccctt taaatatttc ccggtaagtt cagtgggttt
    16501 aaaaaatggc tgatctacaa gttcgtttgc tttgggtcct atttccttcc ttagttcgtt
    16561 caaaatttct tctttttctt cctctgttac ttcttctcga aactcaagat aaggcgtttt
    16621 tccaatcatc tcaattgcct catctggatc tgtaattcct gccagttcca cacttaaacg
    16681 atattcatca ccagattttt gaacctgtac tatgggctct tgaacaccaa aaagattaac
    16741 tcttctttca atcacatctc tcaatccttc cattgaattt tgccagtcct cttttgaaac
    16801 ctgagaaaga tctgcttggt acaaaagatg cactcctcct tgaagatcaa gtcctaattt
    16861 atagggtttt tgcataaatt ccggtacaga gatatttaat ctttctgcta ttctcccagg
    16921 catagcaaaa agagctaaaa aaatagctaa gaccaaaaca ataactacaa aaatccaatt
    16981 ttttgtttgc ttgttcattt ttttatttaa ctataaatta tagcagttaa aaacaaaaaa
    17041 taaaagtatt ctccacaaaa atttaaaaaa tcaaaaccgc cattctattc ttctggcgga
    17101 atgcctaaat aattaaacaa atactcattt atttcttgag caaagggccc taaagaatct
    17161 gaagaaaatt gcggtccttt tgtcggcgca tccattttta aaatgaaaat aaaggcaggg
    17221 tcaaaagcag gagcaaaatc aacaaatgat tgaataaaaa aagaagaata acccccctta
    17281 ttttcaccaa gataactcca agatgcttgg gctgtaccag tttttcctgc gatcaaatat
    17341 ccctctacct cagctttttt gccatatcca ttttttacag cactcaccat cattgcagtc
    17401 accctcgatg ctgtttcagc agatattact tgaccaatta cttcggtttc aatttcttct
    17461 tctgtgccgt cagaggaatg aataattttt tttactaaat ggggttttac catttgtcct
    17521 tgattggcaa tagcagtaaa agcagttaaa atttgaatag gtgtagcact aatgccctga
    17581 ccaaaagagg cattggcata ttcaatagcc ccatcaccag attgaggatg aagaatattt
    17641 gcaatactcc ctgtttcctc tcctgaaaga tcaatcccag tttttgtgcc aaagccaaat
    17701 ttttttacat aatcaagaaa tttatcatta cccacttgct gttgcataaa aacagcaccc
    17761 gtgtttaaag aaagctcaat tacattagtc attgtttggg taccatggga ttttaaatca
    17821 gaatttctaa tcgtatggct tccaatttta atttcccctg aatcataata agttgtttgg
    17881 ggagtaactt ttttttcctc aatagccatt gctgcggtaa agggcttaaa aattgaacct
    17941 ggttcatagg ctaaaatagc aggattttta aacactgaaa tgttttcttt ttcttcataa
    18001 ttatttggat caaaattggg aagagaagcc attcccaaaa cctcaccggt cttgggtgca
    18061 agtactataa tagtagcatt gcgaggagca tatttttcaa atatctcctt tgtttttatc
    18121 tcaagaaaca attggatatt aggatctagt gttaaaataa gatcattgcc atcaaccggt
    18181 tcctgaattt tggtaagggg taagataaat tttccctttg catcttttac tcctttccga
    18241 taaccatcca ctcctcttaa ttcttcttgc cagtactgct caattccata ttgtccttta
    18301 ttctctttgt tgtaaaaacc caacacatga gaagccattc tctctcgtgg ataaaatcgt
    18361 tcaagatacg attcaaaatg aacaccggca atattcttta ttttctctcc ctcaattgcc
    18421 ggaatttctt tcaaaaatat ccaaggatca tctatttgag atgctttttc aaaaacatct
    18481 gctttttcca tttttaaaac atcagccaat atttcagacg ttttttcaat gttctttatc
    18541 tcccgaggta ctataaaaac tttttttgta ccaatattag ttgctaacac tgtactctcg
    18601 tcagagaaaa aaatttctcc tctttctgct tttaaatctt cctcgtcaat ctgctgtaaa
    18661 tcagccaatt ccttatagtg attatactga tttacttgga tgtaaaataa gcgaccaata
    18721 atggtcgctc ctaaagaaaa aaataaaaac attaaaacat aaagatgaga attttgagaa
    18781 ttcattttca aaatgaacta tcttttttct tttgggcttg cctgagataa actactactt
    18841 gtggaaaatt gaagatatct tatatcgcct gcccttacca attctaggct ttgaaccttt
    18901 tcctccattg cctccaaaga taaaccctcc ttaatttctg tttcaagaat tttattttca
    18961 tcttgaagga ctaatagttt attttctttt gcccttaaat caaaattaat acttaccatt
    19021 tgaatagtaa aataaatata aagacaaaag caagctataa aaagaagcag aaaaattatt
    19081 gataaaacca ttgataaatt aatcctactg ccacgtgagg tcttaatatt tttggcaaca
    19141 tcttttcttt ttcccgataa agatgttttt gatatagtat aagtatagag ggcttgtggc
    19201 atatatttaa atttttatta tgttaaatat tttttaaaaa gttaatttaa ctgctgctct
    19261 taattttgca ctgtgagatt ttttattttt ttcaatttct tcttgacttg gtatcacagg
    19321 cttttttgtt aatattttta tttttccttc ttttgctttc tccttaaaaa aatttttgac
    19381 aatccgatct tccaatgaat gaaaagagat cactactatt cttcctcctg gactcaaaat
    19441 ttctaatgct tggggcagtg ccacttttaa attttccaat tcttgattga ccacaatcct
    19501 taaagcttga aatgttcgtg ttgccggatg aatacgtcct ctctcataat ttttaggcac
    19561 tgcttctcga attacctcaa tcaagtcaaa agttgttaaa atgggttttt gtttccgttg
    19621 ctttgcaatc ttgtcagcaa tacgccaggc atatcgctcc tctccatatt ccttaataat
    19681 tctttctatt tcttgagaag gccattgatt tataatctcc ttagcgctta ctgtagcagt
    19741 ttttccgtag cacatatcaa gatcctcatt gtatcgaaaa gaaaatcctc tctttgattt
    19801 ttccaagtgc catgaagaca ttcccagatc aaacaaaatt ccccttatct ttccttgcca
    19861 atcaggacaa aaattttcta caattttttt taaattttta aaattatcat ttaccaaaat
    19921 aaccctatta tctgaatctc tctttgttaa tttataattt ttaatttgct ctggatcggc
    19981 atcaatagct aatacctttc cgttgggagc attcttttcc aaaattgcta ttgtatgacc
    20041 accttctccg actgtgcaat caataaaatt atcatttggt cttggatcaa gatagtaaag
    20101 aacctcttca actaaaactg gaacatgaac cataacattt attaatttct aatttctaat
    20161 ttctaaaccc tgaggatttg cctcttttcg cacttcgctt ccctttaaaa agttcggcaa
    20221 ttgaaaatta aaaatttttc acttttcatt ctaaatcaac tcactaagtc tttctgccat
    20281 atctcccact tctttttctg atcttgattg atattcttcc cac
//
LOCUS       DAOTQD010000005        19796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_2806_length_19796_cov_8.002938, whole genome shotgun sequence.
ACCESSION   DAOTQD010000005 DAOTQD010000000
VERSION     DAOTQD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19796
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_19796_cov_8.002938"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2625
                     /gene="secA"
                     /locus_tag="P5570_00435"
     CDS             91..2625
                     /gene="secA"
                     /locus_tag="P5570_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRR39218.1"
                     /translation="MSFFSKIFGDQNEKALKKMQPIVEKINNLESKIQRLSDEKLKEK
                     TVDFKDRIKKGEVLDDILPEVFAVVREAAKRTLGQRHFDVQLMGGIAMHQGKITEMKT
                     GEGKTLTATLPLYLNALEEKGCHLVTVNDYLARRDTVWMGQIYHFLGLSVGCLNHEQS
                     FVYDPEYKKSAEEKDKIRDELGSFQVVEDFLRPVSRKEAYLADITYGTNNEFGFDYLR
                     DNMVYNMDQLVQSRNNENDEDIYHFAIIDEVDSILIDEARTPLIISAPDTESSKWYQE
                     FARIIPKLNSEIDFKVDEKRKAVTLTEEGINKIEKLLGKENIYEERGIKYLHHLEQAL
                     RAEALFKRDRDYIVKNNQVIIVDEFTGRLMPGRRWSGGLHQAIEAKEGVPVQAESLTL
                     ASITFQNFFRMYKKIAGMTGTAVTSAEEFQKVYGLDTVMIPTHKPMIRKDLPDKIFKT
                     QKGKYQAVVEEVKNRHSQGQPILIGTTSIDKNEHLSRLLKAEGISHEVLNAKNNEKEA
                     AIIAQAGKRGQVTVATNMAGRGVDIILGGNPPDQKEAEEIKKVGGLCVIGTERHEARR
                     IDNQLRGRAGRQGDPGVSQFFISLEDDIVRVFGGDTIKRVFERFDFPENMPIENKMIS
                     RAIESAQARIEGYNFDIRKHLLEFDDVLNKQRIKIYKKRREILKESEKLSFATAKRDV
                     KEGEQTSFCSSNLKSQILEMAKTPEEKKALEAKEKELGENFFKVAKFIMLNTLDTLWM
                     QHLEDMETLRDSVQLRAYGQIDPLVAYRTEGHKMFQSLLEDFEEKVLDTILKAKIKPN
                     IKMTSNKITQPVSSATKNAYKKVGRNDPCPCGSGKKYKKCCYPKFG"
     gene            3033..3212
                     /locus_tag="P5570_00440"
     CDS             3033..3212
                     /locus_tag="P5570_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39219.1"
                     /translation="MPSPRALEKSGVILFFSLLSLIAAIIGFINGAECGNWLCSYFFI
                     GLAVILFFWGFEVAQ"
     gene            3227..4888
                     /locus_tag="P5570_00445"
     CDS             3227..4888
                     /locus_tag="P5570_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRR39220.1"
                     /translation="MVFNPKESERRLAIIRIQQQEKKAREMAILHNLPYIDLTVTPID
                     LDALALVPKEKAEKGLFVPFQKIEKRILFATIDPQNPLLKETTEKFKNKGFIVEILIC
                     SKRSLERALKEYSKVPTSSKEVVGKIKIKNERIGLIEKEIKNIADLKSRLENISKEGN
                     GWVLEMILGSALALRVSDIHIVSALEGANLRYRIDGILYDITLLNEPMYHSLLNRLKL
                     LSGLKVNIEDIAQDGRFSIFAKEQEIEVRLSVIPGEYGEDIVMRILNPDMILSIEELG
                     LHPWHQEDILREIRKPTGMILICGPTGSGKTTTLYACLKKVATSKVKVITIEDPIEYH
                     LENVDQTQIEPEKGYGFTNALRAAMRQDPDIILIGEIRDGETAETAIQAALTGHLVFS
                     TLHTNDAAGAIPRLIELGANPTSIAPAFNLIVSQRLLRKVCKKCAKKIIIDEKTKEEI
                     KKELESLPLEIKPNLEGNIEIFQPQGCVDCNNSGYKGRIGVFEILKKTPIVEKTILTI
                     PSISIMREIAIQEGMITIRQDGILRVLEGITTIEELERVLGPIEE"
     gene            5125..6813
                     /locus_tag="P5570_00450"
     CDS             5125..6813
                     /locus_tag="P5570_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRR39221.1"
                     /translation="MKMNLSRVKKKILIFILAIPFLVLPFELAWGANPGDVVINEVMW
                     AGADNEWIELYNNTDEEINLEGWSIEGAKTSGGSLEITCANSSCIISARGYFLITDEK
                     SGLDANLKTSISLTDDKGNGNLVLKDKNKNQIDVVKGDAWPAGKKDTTNKKYYSMERV
                     NPTISGDQICNWRTTNTETICDTYTSSQGTYNIYGTPKAKNSQYSEIVCQSLVANIQT
                     TEIISVTNNNITFDASASTGDIQSYSWNFGDGETATGKIVTHKYQYPGEYYVSLIISD
                     GEKESSDLKKVTIFSSEIFISEFLPNPKGDDSGKEWIEVVNESSNVQNISGWGLGNSS
                     EKPKFIFPSGSYLASQGLILLSSSLTKISLSNDSGSLFLFYPSGSISQEVKYENPGED
                     VSIARKDGEYFYTKNLTPGMKNIVSLSFKEVQSNSASQAQSLSQSSTSTSTSTQSQNQ
                     SQPQNPSEASTQNLTPNQESASFNSKVSEENEDLALQDQTVAVPTNTLLQDVLNKNLL
                     AEVKANQTKLILSIVCVTLCSGFFGVSLVILRRKLKNKNIGDKNIEKIEVEIEK"
     gene            6908..7639
                     /locus_tag="P5570_00455"
     CDS             6908..7639
                     /locus_tag="P5570_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR39222.1"
                     /translation="MSGHSHWAGIKHKKGLEDAKRAKIFPKMSRLISVTAREKGGDPE
                     TNAELKMAIERAKSFNMPQENIERAIKRGSGELGEEKLESFLLEGFGPGKIAVLIEGV
                     TDNKNRTIAEIRHLFENFGGKLASGGVLWMFERKGIITIAEHQEYKDKKDDLELLVIE
                     AGAEDIEWNENEVLEVYTKAEDLNQVKKNLEEKGIKIENINLGFKAKEEIRIEDKETK
                     DKIEKLFESLDERDDVQEIYSNLAV"
     gene            7669..8010
                     /locus_tag="P5570_00460"
     CDS             7669..8010
                     /locus_tag="P5570_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39223.1"
                     /translation="MPKESEIRKKVVQILENDGWIVWYPPKVRYKKTDIFGIIDLLAL
                     KGRRQKNIQLTTVSNLATRRKKIDNFLTKFEIEMTVEIWAWYAKKKEFKKEKVRFDLP
                     KKPKKKKPKII"
     gene            8034..8687
                     /locus_tag="P5570_00465"
     CDS             8034..8687
                     /locus_tag="P5570_00465"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HRR39224.1"
                     /translation="MSFLVEQIVNFGVSEGTLALILMLPVVATFIAFARQIIGIRGFG
                     IYITLIIAYAFVATKLKYGIVIFLVVVIAGTLMRRLLQKIKILYLPRMAMVLTFVALA
                     VFLMFLLGSYLNIEGLQVISIFPILIMTLVVERFVAAQMEKGQRTAIFMTLETLILAI
                     ISYYIITWTSLQQLLLEWPIVSIFVVLILNFALGHWTGFRLSEYFRFKELMEYLPKK"
     gene            8711..10063
                     /locus_tag="P5570_00470"
     CDS             8711..10063
                     /locus_tag="P5570_00470"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HRR39225.1"
                     /translation="MYGILGINARNQKYLDSSKKIKKTLDSKLLTKNILKEAGLPVPN
                     TLALIKTRSELFNFNWEILPPTFALKPNRGFGGQGIIVVYGKKKKTIEPTWIRSDGSL
                     VRKEDLEVQILDIMEGNFSLFSLPDVAFFEERIKVLKELKPYSWKGIPDIRIIVFEGV
                     PVMAMLRLPTPESRGRANLHQGAVGVGIDLGTGLTTNAVWHNKFIDYYPGTRYLLRGI
                     KIPFFKEILRFSINAQAAMGIKFLGVDVAIDRERGPVILELNARPGLSIQMANLAGLE
                     ERLKRISGLNVKSSEKGVRLAQDLFGESKEDQLIETIDDKKLEPLLGRKVLGPIEEVE
                     IVPHLDEEKTNDNYLNERRYYKTIAKIDTGAWRSAISFEIAKKLKIKEGGELRMVKSA
                     LGEEIRTITPLVFFIKGEKIETEVFLADRSQMKYEMIIGRRDLKRFLIDPAKKIDIKK
                     "
     gene            10080..11060
                     /locus_tag="P5570_00475"
     CDS             10080..11060
                     /locus_tag="P5570_00475"
                     /inference="COORDINATES: protein motif:HMM:NF023483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3541 domain-containing protein"
                     /protein_id="HRR39226.1"
                     /translation="MNISPQKVASKIESTYEKNIFSFPLKYQFHFFLRLFRITGDKKY
                     LMPILTYQKLRAIETIQMIKNRNQKLFLEKKSNNLFKKLPKRGKKNKKRREVFQKNKQ
                     ILFYFYLVESLYQWKVFKISENEFFQKYYNEGIEYLSKIDFGKIIFEKDLFLHYSAQV
                     VNQVYYLEYLGISTLKERFLKDFQKIFFQKEKESLYDFKNKIYGLTHLIITDSDYYQR
                     FVSKKKNKWILDYFEKNLSRIFKETNPDIVSEVGLSFKLCRENSPAVLKKIKNYLIFS
                     FDQKRGYIPQKDTRENNDLQKAEHRNIIALMFLKDFKKLYKGPNLIKFTF"
     gene            11159..12583
                     /locus_tag="P5570_00480"
     CDS             11159..12583
                     /locus_tag="P5570_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39227.1"
                     /translation="MKLSFLRKKHPQFIYQSFSYQLKRNNLIITFDFRIPPDFKFSPQ
                     LKIKNIDKKRFQKIKKETLENLIFNLGMVESLSYWKATLSPKINVEAGYLTKEQISWW
                     QDLILKGMGQFFYENKIDFTKSNFLKIKSSQKSLNFDGPEKLQLKDRILIPLGGGKDS
                     IVTLEILKEAEEEFKIFSLNPTEAIKKIIKIGKEKSPIVIERKIDPLLLKLNQKGYLN
                     GHTPFSAYLTFLTALCAVIFDFKYVAFSQERSSNEGNIKYQGRIINHQYSKSFEFEKK
                     FRDYLEKYLVKGVYSFSFLRPLYEIQIAKLFSCYPQYFSAFLSCNEAQKTYSGTKRPI
                     QKWCGQCSKCLFVFTILYPFMEEKTLIKIFHKNLFKDQNLLPLMQELIGIKDFKPFEC
                     VGTKKESKIAFYLAWKKKKKSGGELPFLLMWFERNIFPREKNWENISKEIMQGFDQNH
                     FLPKEFERILKKKLKKPAQTLDKF"
     gene            12626..13732
                     /locus_tag="P5570_00485"
     CDS             12626..13732
                     /locus_tag="P5570_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRR39228.1"
                     /translation="MNICTISKTESSEGSKGCKPVRKGRFKRLLLREAVVLVWLFLMF
                     FVLPEQIVLAETGSVKMSLSNIEVAQQTKTNLEKNSSAIQSLEEHFLSNQEEGIVITP
                     NTPVIKNLIQDQELLKKENKEQGQEQEQRKESVEYTIKEGDTISGIAASFGLKTQTIL
                     WANNLNDSSIIRPGDSITIPPADGIIYTVKKGDTLGKIAQDYKSDLNEILDWNQLDND
                     KIVEGQKLFLPGAKKPVIVVKTSPVSSSSSPVTSNVVPRKAPVYSSCHQFPYGYCTWY
                     VAQKRCVPWGGNAKDWPSNAQAYGYSVCWGSNCDCPAGSILVTRESWYGHVSYVIGQT
                     NTTITISEMNRYGWAKVNTRTINKGDWIIRACIY"
     gene            13737..14507
                     /locus_tag="P5570_00490"
     CDS             13737..14507
                     /locus_tag="P5570_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HRR39229.1"
                     /translation="MSNLLKLVMEGLMVGVAIFTPLYIDPHSTEGLSELNPCYAEGID
                     EMDKEEILIIDEGLEYKIEKNGGTIEEILKNAQIEINEEDIIFPAKEIVFQSGTNIII
                     QRATPVILNFYGKEQKIFTQKEKISEVLIEKGIEIKENDLINVDLEKSIFPGIEIKIW
                     QKPKPKEPEIAKTGRSQTGIGSWYNFIPGDFCASTAFKKGTKLLVTNLENGRQLVVTV
                     NDYGPVNSRIIDLEKTAFAKLAPPSKGVIKVKVEEIHH"
     gene            complement(14569..14639)
                     /locus_tag="P5570_00495"
     tRNA            complement(14569..14639)
                     /locus_tag="P5570_00495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14605..14607),aa:Pro,seq:ggg)
     gene            complement(14655..14840)
                     /locus_tag="P5570_00500"
     CDS             complement(14655..14840)
                     /locus_tag="P5570_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HRR39230.1"
                     /translation="MRKCAICGKTYIKSIPLNKLRGKYNPAPKKRKYPNLQWVHLSNG
                     KRVKACTQCIKTLSKVK"
     gene            14947..15588
                     /locus_tag="P5570_00505"
     CDS             14947..15588
                     /locus_tag="P5570_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRR39231.1"
                     /translation="MDVFILIFIFLIFYFSMTVHEVAHGAVAGMLGDPTAKLAGRLTL
                     NPLKHLDPIGSVILPAFLILTSAFNGGSGIIFGWAKPVPYNPNNLYDKKYGRAKVGAA
                     GPLANLLVAIVFGLCLRFLPMTENAFFQNLQMGFIYIVWINLLFFVFNLWPAPPFDGH
                     HILFSFFPSLETKIGFLKNPFFSMLMAILFMSIIGFPYICPWLFQLLTGMNIF"
     gene            15630..15803
                     /locus_tag="P5570_00510"
                     /pseudo
     CDS             15630..15803
                     /locus_tag="P5570_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
     gene            15900..18560
                     /gene="dnaG"
                     /locus_tag="P5570_00515"
     CDS             15900..18560
                     /gene="dnaG"
                     /locus_tag="P5570_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRR39232.1"
                     /translation="MPAIKTLRDYEGEVIDLKLRKSNEVVSLTLDHEVYVIKTRHCKY
                     KNRLTRICNWNCKKSCPAKFYQNYKIEKLPASQLSIGDYLLYPINQEIRDIEFLNLEK
                     YSTWRKGVFGFNPKTIPLKIKVDKNFLKLIGYYIAEGSNHRAYIRFSLGSHEELFAKE
                     IRELIEDIFKIKTTTYKRKKEGKTGLEITACNSKLANIFGNLCGKGAANKHIPFEFQY
                     LPSEKQKTILEAIHKGDGWTKEGKIKYIDTISLILAEQIRDILLRLNIAPTFWIHQKK
                     IDKKGTHHKKSFTVMWQEHYQLNYSQFYNEPGGTEYWLLPIENIKKKSYKGNVYNLVV
                     DKDHSYTTSNFVVGNCGKSGDIFKFIMDIEGVEFKEALEILAKKAGVELKKESPQKRT
                     ERQRQYEICEIATKFFEAQMTRTNAGKKAKDYLLSRGISEKSIKEWRLGYAPNSWQGL
                     SNFLVGRGYKREEIVKAGLAVQRENISGNSQDISNYSRGSYDRFRGRIMFPIFDIQGN
                     VIGFGGRITEEQNEKMKKEKGGAPAKYINTSNTLIYDKSRVLYGLHKAKTSIREKDEI
                     VLVEGYTDVILSSQAGAKNVVSTSGTALTPLQLKILSRYSKNLTLCFDMDIAGQAATK
                     KGIDIAQIQGFNIKIVSLPDEKDPADVIKENPQEWLNLIKESKEIISFYFEKCLSHFD
                     PGKIEDKKKIAETLVPLISRIQNKIEQGYWIQKLANAINLNEEVVWGEVKRIISTHQF
                     PSNSSNNSNEIGDSFAKISEQKKDQQRQIEERLLMMFLQTPEETKNIGPIKFSFPEGQ
                     IIFNALKEKKKIPKELEELVAELELQFDIEKEKDSTMDVKKEIEFCILRLKEFSRQKK
                     LQRIIKEIKEAESKNNKEKVSKLLEKLNKI"
     gene            18568..>19796
                     /locus_tag="P5570_00520"
     CDS             18568..>19796
                     /locus_tag="P5570_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR39233.1"
                     /translation="MKKQTKKTKKNKTSLPSSKTKNKNKKTKRTKPLSQNKLKNKTTT
                     TESKAKKLQSKKKSEAFSRSRIAIKEEALKELIKRGKSRGFVTLSEVLKFFPRAEEDI
                     AGLEEIYQKLESEGLRVEDSVDFLGDIDLASSPEQIPEIDSIQKYLKEIGRYPLLTLE
                     EEKELSQRLERGDKAAQKKLIQSNLRLVVSIAKKYLGRTPHLTLLDLIQEGNIGLFKA
                     VEKYDWRKGFRFSTYATWWIRQSITRAIADQSRTVRLPVHIVERLSKYNQIKKRLLQE
                     LGREPLAEEIAIEMHEDVKKVKNLEKVAMGTVSLEQPIGDDDKEGTLEEFIEDKKTAS
                     PAETTSLSILRDQIKEILSDLAPREQQILKMRFGIDGEIPHTLEEVGKEFNVTRERVR
                     QIQQRAIEKIKEHKKVK"
BASE COUNT         7233 a         2670 c         3726 g         6167 t
ORIGIN      
        1 tctttcaatg tggcttctgg gacgggattg cttcgcttta attacaatta ttgtaaattt
       61 ttattaggtt aatttagaat ttttattttt atgtcttttt tttctaaaat ttttggagat
      121 caaaatgaga aagcgttaaa aaaaatgcag ccgattgttg aaaaaatcaa taatctcgaa
      181 tcaaaaattc aaagattatc tgatgaaaaa ttaaaagaaa aaacagtcga ctttaaagat
      241 agaattaaaa aaggagaagt tcttgatgat atcttgcctg aggtttttgc tgtagtgcga
      301 gaggcagcaa aacgaacctt gggacagcgt cattttgatg ttcagctaat gggtggcatt
      361 gcgatgcacc agggcaagat tacagaaatg aaaactggtg aaggaaaaac tcttactgcc
      421 actttgcctc tatatttaaa tgcccttgaa gaaaaaggat gtcatttggt tacagtaaat
      481 gattatttgg caagacgtga tacagtttgg atgggacaaa tttatcattt tttgggactt
      541 tctgttggct gtttaaatca tgaacagtcg tttgtatatg atccagagta taaaaaatct
      601 gcagaggaaa aagataaaat tagagatgag ttgggtagtt ttcaagtggt tgaagatttt
      661 ttaaggcccg tttcgagaaa agaagcgtat ttagctgata ttacttatgg caccaataat
      721 gaattcggat ttgattattt gcgagataat atggtttata atatggacca gttggttcag
      781 tcacgcaaca atgagaatga tgaagatatt tatcactttg ccattattga tgaggttgat
      841 tcaatcttaa ttgatgaggc ccgaacacct ttgattattt ctgctcctga tactgaaagt
      901 tcaaagtggt atcaagaatt tgccagaatt attcccaagc ttaattctga gatcgatttt
      961 aaagttgatg agaaaagaaa agcagtaaca ttgaccgaag aaggcattaa taaaattgaa
     1021 aagcttttag gaaaagagaa tatttatgag gagcgaggga taaaatatct tcaccatcta
     1081 gagcaagcct taagagcaga ggcactgttt aaaagggaca gagattatat tgtaaagaat
     1141 aatcaggtga ttattgtaga tgaatttacg ggcagattga tgcctggcag gagatggtct
     1201 ggaggactcc accaagccat tgaagcaaaa gaaggtgttc ctgttcaggc agagtcattg
     1261 accctggcgt ctattacctt tcagaatttt tttagaatgt ataaaaaaat agctggaatg
     1321 acaggtactg ccgttacttc ggctgaggaa tttcaaaagg tttatggact ggatacagta
     1381 atgattccca ctcacaaacc aatgattaga aaggatttgc ctgataaaat ttttaaaact
     1441 caaaaaggca aatatcaagc agtggtagaa gaggtaaaaa atcgacatag ccaaggacaa
     1501 cccattctaa ttggtactac ttctattgat aaaaatgaac atttatcacg attgctaaag
     1561 gcagagggaa tatcacatga agttttaaat gctaaaaata acgagaaaga agcagctatt
     1621 attgcgcaag caggaaaaag gggacaagtg acagtggcaa ccaatatggc tggtaggggg
     1681 gtagatatta ttttaggagg aaatcctcca gatcaaaaag aagcagaaga gattaaaaaa
     1741 gtggggggac tttgtgtgat tggaactgaa cggcacgaag cgcggcggat tgataatcaa
     1801 ttacggggaa gagctggtcg gcaaggagat cctggagttt ctcaattttt tatttctctg
     1861 gaagatgata ttgtaagagt ttttggtggc gatactataa aaagagtatt tgagcgtttt
     1921 gactttcctg aaaatatgcc aattgaaaac aagatgattt ctcgagctat tgaatcggct
     1981 caggcaagaa ttgaagggta taattttgat attagaaaac atcttttaga gtttgatgat
     2041 gtattaaaca aacaacgaat aaagatttac aaaaaaagga gagagatact taaagaatct
     2101 gaaaaattga gttttgcaac agcaaaacga gatgtaaaag agggagagca aacttctttt
     2161 tgctcatcaa atttaaaatc tcaaatcttg gagatggcaa aaacgccgga agaaaaaaaa
     2221 gcccttgaag caaaagaaaa agaacttgga gaaaattttt tcaaagtggc aaagtttata
     2281 atgcttaata ccctggatac attatggatg caacatcttg aagatatgga gacattgaga
     2341 gacagtgtac agcttagggc ttatggtcag attgatcctt tggttgctta tcgaacagaa
     2401 ggacataaaa tgtttcaatc acttttggag gattttgagg aaaaagtatt ggatacaata
     2461 ttaaaagcca agataaaacc caatattaaa atgacttcta ataaaattac acagccagtc
     2521 agttctgcta caaaaaatgc ttataaaaag gtagggcgta atgatccttg tccttgtggc
     2581 tccggaaaaa aatataaaaa atgttgttat cctaaatttg gataaaaagt gatgtttatt
     2641 gattttttat taaaaataaa ttaaatttaa tattaaggta ggttcaagaa cagaacgcaa
     2701 caatcctttt ttgtctttga cacgagcgaa gcgtagcgca gcgaagtgtc gattcccttt
     2761 atgtcattgc gagcgaccga aggaagcgaa gcaatctcgt ggtaatactc tgggattgcc
     2821 acgtcctact gcggtaggac tcgcaatgac agtagacgcg cgccacggga tcgccacgcc
     2881 cttcgggctc tcaaagaaaa tttcattttc ttttcgacga gctcgcttcg ctctctcgtt
     2941 cgcgatgaca gcgggcgaag cgtttaaaaa aaattaaaaa ttaaaaatta aaaattaaaa
     3001 attaaaaatt ttcatttgag tttttgaaaa ctatgcccag tcccagagct ctagaaaaat
     3061 ccggcgtaat attgtttttt tcattgcttt ctcttatagc agcgattatt ggttttataa
     3121 atggtgctga gtgcggtaat tggttgtgct cttatttttt tattggtttg gcagttattc
     3181 tttttttctg gggatttgaa gttgctcaat aaattcaatt tgttttatgg tgttcaatcc
     3241 aaaagaaagc gagcgtcgtc ttgcgattat tcgaattcaa cagcaagaaa aaaaggcaag
     3301 ggagatggct attttgcaca atcttcctta tattgattta actgtaactc ctattgatct
     3361 tgatgccctt gctttggtgc caaaagaaaa agcagaaaag ggattatttg ttccttttca
     3421 aaaaattgaa aagaggattt tatttgctac tattgatcct caaaaccctc ttttgaaaga
     3481 gacaacagaa aaatttaaaa ataagggatt tattgttgaa attcttattt gctctaaaag
     3541 atcattagag agggctttaa aagaatatag caaggttcct acttcttcta aagaagtagt
     3601 aggcaagata aaaataaaaa acgaacgtat tggcttgatt gaaaaggaaa taaaaaatat
     3661 tgctgattta aaaagcagat tagaaaatat atctaaagag gggaatggct gggtacttga
     3721 aatgatttta ggaagcgcat tggctcttcg tgtttctgat attcatattg tatctgcttt
     3781 agagggagct aatcttcgtt atagaattga cggaattttg tatgatatta cattattaaa
     3841 tgaaccaatg tatcattcat tgttgaatcg attaaaactt ctttcagggc ttaaggtaaa
     3901 tattgaagac attgctcagg atggaagatt tagtattttt gccaaggagc aagaaatcga
     3961 agtacggctt tcagtgattc ctggtgaata cggagaagat attgtaatga ggattttaaa
     4021 tccagatatg attctttcaa ttgaggaatt aggtttacat ccttggcacc aagaggatat
     4081 attgcgagaa ataagaaaac ctacgggaat gattttaatt tgtggaccga caggatctgg
     4141 taaaactact actctttatg cttgtttaaa aaaagtagcg acatcaaagg taaaagtaat
     4201 tactattgaa gaccctattg aatatcacct tgaaaacgtt gatcaaactc aaatagaacc
     4261 tgaaaaggga tatggtttta ctaatgccct aagggcagca atgagacaag atcctgatat
     4321 tattttgatt ggagaaataa gagatggcga aacagctgaa acagctattc aagctgcttt
     4381 gacaggacac ttagtatttt caactcttca taccaatgat gctgcgggtg caattcctcg
     4441 attgattgaa ttgggagcaa atcctacttc tattgcccct gcttttaatc ttattgtgag
     4501 tcagcgtctt ttaagaaaag tttgtaaaaa atgtgcaaaa aagataataa tagatgagaa
     4561 aacaaaggag gaaataaaaa aagaactaga aagtcttcca ttagaaatta aacctaatct
     4621 tgaaggaaat attgaaattt ttcaacctca gggttgtgtt gattgcaata attctggtta
     4681 caaaggaaga attggcgttt tcgaaattct taaaaaaaca ccgatagttg aaaaaacaat
     4741 tttaacaatt ccatcgattt ctataatgag agaaattgca attcaagaag gaatgataac
     4801 aataagacag gatggtatat tgcgggtttt agaaggaatt actactattg aagaattaga
     4861 aagggtatta gggccgatcg aagaataaaa taaaaaaacc gccagtggaa taataaaaag
     4921 ttcttttata aaaaagaaaa attagaattt ttccgaggag tggtttggct ttagtccatt
     4981 gagctgggtt gccccaagtc tcaggagaaa ctaaaccatc cgaatactaa gaaaaattcc
     5041 agtgggcaat tttaaaataa ctccttttag gggttttttc taatcagccc ttattccact
     5101 tgcggtcttt attatttata gcatatgaaa atgaatttgt caagggtaaa gaaaaaaata
     5161 ttaattttta ttttagcaat tccatttttg gttttgcctt ttgaattagc gtggggagct
     5221 aatcctggtg atgtggtaat aaatgaagta atgtgggcag gagcagataa tgaatggatt
     5281 gaactttata ataatactga tgaagagatt aatttagagg gatggagtat tgaaggagct
     5341 aaaacaagtg gaggatcatt agaaattact tgtgcaaatt cttcttgtat aatttcagca
     5401 cgtggctatt ttttaatcac tgacgaaaaa tcaggacttg atgctaattt aaaaacttca
     5461 atttctttaa ctgatgataa aggaaatggt aatttagtgt taaaggataa aaataaaaat
     5521 caaattgatg tagtaaaagg agatgcatgg ccagccggta agaaagatac tactaacaaa
     5581 aaatattatt caatggagcg agtaaatcct acaatttcag gagatcaaat ttgcaattgg
     5641 agaactacca atactgaaac aatctgcgat acttatactt catctcaggg aacatataat
     5701 atttatggca cccctaaagc taaaaattct caatattctg aaattgtttg ccagtctctt
     5761 gttgctaaca ttcaaacaac agaaataata agcgtgacta ataataatat tacttttgat
     5821 gcgtcggcct caactggaga tatccaaagt tattcttgga atttcggaga tggtgaaaca
     5881 gcaacaggta aaattgttac tcataaatat caatatcccg gcgaatatta cgtttcctta
     5941 ataataagcg atggcgaaaa agaaagttct gacttaaaaa aagttactat tttttcttct
     6001 gaaattttta tttctgagtt tttgccaaac cctaaaggag atgattctgg aaaggaatgg
     6061 attgaggttg taaatgaatc aagtaatgtg caaaatattt ctggttgggg attagggaat
     6121 agttctgaaa aacctaaatt tatttttccc tctggtagct atcttgcttc gcaaggtttg
     6181 attttacttt cttcgtcttt aactaaaatt tcactttcaa acgatagtgg ctctttattt
     6241 cttttttatc cctcaggaag catttctcag gaggtaaaat atgaaaatcc aggagaggat
     6301 gtttcaattg caagaaaaga cggagaatat ttttacacga aaaatctaac ccctggaatg
     6361 aaaaatatcg tttctctttc tttcaaagaa gtacaatcaa atagtgcttc tcaagcccaa
     6421 tccttatctc aatcttcaac ttcaacttca acttcaactc aaagtcaaaa tcaaagccaa
     6481 cctcaaaatc cgagcgaggc ctcaactcaa aatttaaccc cgaatcaaga atccgcttct
     6541 tttaattcaa aagtcagtga ggaaaatgag gatctcgctt tacaagatca aacagttgct
     6601 gtaccaacaa acactttgct ccaggatgtt ttaaataaaa atcttttagc tgaagtaaaa
     6661 gcaaatcaaa caaaattaat cttatcaatc gtatgcgtta ctctttgctc tggatttttt
     6721 ggcgttagtt tggtaatatt aaggagaaaa ttaaaaaata aaaacattgg tgataaaaat
     6781 attgaaaaaa ttgaagtcga aatagaaaaa taatttttcc cgaattttcc tcgagtcgtt
     6841 tcgaattttt ctcgaatatt cgagatttat tcgttttcat tcgtagatca ttcgagagat
     6901 attttttatg tcaggtcatt cacattgggc aggtataaaa cataaaaaag gattagaaga
     6961 tgcaaagcgg gcaaagatct tccctaagat gagtcggtta atttcagtta ctgctaggga
     7021 aaaaggagga gatcctgaaa cgaatgcaga attaaaaatg gctatcgaga gagccaagtc
     7081 ctttaatatg ccccaagaaa atattgagcg ggcaataaaa agaggatccg gagaattagg
     7141 agaagagaaa ttagagagtt ttttgctgga aggttttggt ccaggaaaaa ttgcagtttt
     7201 gatagaagga gtaactgata ataaaaacag aactatagct gaaattcgac acctctttga
     7261 aaattttgga ggaaaattgg caagcggtgg agtattgtgg atgtttgaaa gaaaaggaat
     7321 tattacaata gccgaacatc aagaatataa ggacaaaaaa gatgatttgg agttattagt
     7381 tattgaagca ggagcagagg atatagaatg gaacgagaat gaagtattag aagtttacac
     7441 taaagcagaa gatttaaatc aggttaaaaa aaatttagaa gaaaaaggta ttaagattga
     7501 aaatatcaat ttgggtttta aggcaaaaga ggaaatcagg attgaagata aagaaacaaa
     7561 agataagatt gaaaaattat tcgaatccct tgatgagcgt gatgatgttc aagaaattta
     7621 ctctaattta gccgtctaac accttttttt aattttacat tctaatctat gcccaaagaa
     7681 agtgaaatta gaaaaaaagt agtgcaaata ttagaaaatg atggttggat tgtgtggtat
     7741 cctccaaagg tccggtataa aaaaacggac atttttggta ttattgattt attggcatta
     7801 aaaggtcgaa gacaaaaaaa tattcaatta acaacagttt ctaaccttgc tacaagaaga
     7861 aaaaaaattg ataatttttt gacaaaattt gaaattgaaa tgacagtaga aatctgggct
     7921 tggtacgcta aaaagaaaga gtttaaaaaa gaaaaggtac gttttgattt gcccaaaaaa
     7981 ccaaaaaaga aaaaaccaaa aattatctag caattgagtc ttaaattttt tttatgtctt
     8041 ttttagtaga acaaattgtt aattttggag tatcagaggg tacgttagcc ttaattttaa
     8101 tgttgccggt agtggctact tttatcgcct ttgcccggca aattattggt attcggggtt
     8161 ttggtattta tattaccttg attattgctt atgcctttgt tgctacaaaa ttaaaatatg
     8221 gcattgtcat atttttagta gtagttattg caggaacatt gatgcggcgg ttgcttcaaa
     8281 aaataaagat tctttatttg cctcgaatgg caatggtact tacttttgtt gctttagctg
     8341 tttttttgat gtttcttttg ggtagctatt taaatataga ggggcttcag gtaatttcta
     8401 tttttcctat tttaattatg actctagtag tggaaagatt tgttgctgcg caaatggaaa
     8461 agggtcaaag aacagcaatt tttatgacgt tagaaacttt gatattagca attatttctt
     8521 attatattat cacttggact tcgcttcagc aattattact tgaatggccc attgtttcta
     8581 tttttgttgt tttgatttta aattttgctt taggacattg gactggtttt cgactatcag
     8641 agtattttcg ttttaaagag ttaatggaat atttgcctaa aaaatagttg aagtttgtaa
     8701 attatatttt atgtatggaa ttcttggcat taatgcacgt aatcaaaaat atttagactc
     8761 ttctaagaag ataaagaaga ccctggattc aaaattattg accaaaaata ttttaaaaga
     8821 agccggtttg cctgtgccca ataccttagc tcttattaaa acacgatcag agctttttaa
     8881 ttttaactgg gaaattttac ctcctacttt tgccttgaaa ccaaacaggg gatttggagg
     8941 acaggggatt attgtggttt atggcaaaaa gaaaaaaacc atagaaccga cttggatacg
     9001 gtcagatggt tctttagtga gaaaagaaga tcttgaggta cagattcttg atattatgga
     9061 gggaaatttt tctttattct ctctgcctga tgttgctttt tttgaagaaa gaataaaagt
     9121 attaaaagaa ttaaaaccat attcttggaa aggtatacct gacattcgga taatagtatt
     9181 tgaaggagtg cctgtaatgg ctatgctccg tttgccaacg ccagaatcta gaggaagagc
     9241 caatcttcat caaggagctg ttggtgttgg gattgacctt ggcacgggcc ttaccaccaa
     9301 tgctgtttgg cataataaat ttattgatta ttatcctggt acaagatatc ttttaagagg
     9361 tataaagatt ccttttttta aagaaatttt aagattctct attaacgctc aagccgctat
     9421 gggaataaaa tttttaggag ttgatgttgc tattgatcgt gagaggggac cggtaattct
     9481 tgagcttaat gcaagacctg gtctttcaat ccaaatggca aatttagctg gactagaaga
     9541 acgtttaaaa agaatatccg gtcttaatgt aaaatctagc gaaaaaggag tgagactggc
     9601 tcaagatctt tttggtgaat caaaagaaga tcagttgata gaaacaattg atgataaaaa
     9661 attagaacca ttattaggca ggaaggtctt aggaccgata gaagaagtag agatagtacc
     9721 tcatttagac gaggaaaaaa ccaatgataa ttatttaaat gagcggcgat attataaaac
     9781 tattgctaaa attgatacag gagcttggcg tagcgctatc tcttttgaaa ttgctaaaaa
     9841 attaaaaata aaagaaggcg gagaattaag aatggtaaaa tctgcattgg gtgaggaaat
     9901 aagaacaatt acgccattag ttttttttat caaaggagaa aagattgaaa cagaagtatt
     9961 tttagccgat aggtcacaaa tgaagtatga aatgattatt ggtcgtagag acctaaaacg
    10021 atttttaatc gatcctgcca aaaaaattga tattaaaaaa taaaaattga atctttttta
    10081 tgaatatttc tccccagaaa gtggcatcaa aaatagaatc aacttacgaa aaaaacattt
    10141 ttagttttcc tttaaaatat caattccact tttttcttcg tctctttcga atcactggtg
    10201 ataaaaaata tttaatgcct attttgacct atcaaaagct tcgggcgata gaaacaattc
    10261 aaatgataaa aaataggaat caaaaattat ttttagaaaa aaaatcaaac aatcttttta
    10321 aaaaattacc aaaacgagga aaaaagaata aaaaaagaag agaagtcttt caaaaaaata
    10381 agcaaatttt attttatttt tatcttgtag aatctttgta tcaatggaaa gtttttaaga
    10441 tatctgaaaa tgaatttttt caaaaatatt ataatgaggg aatagaatat ctttcaaaaa
    10501 ttgattttgg aaagattatt ttcgaaaaag atcttttttt acattattca gctcaagtag
    10561 taaatcaagt atattactta gaatatttag gaatttcaac gttgaaagaa aggtttttga
    10621 aagattttca aaaaatattt tttcaaaaag aaaaagaatc attgtatgat tttaaaaata
    10681 agatctatgg gctgacccat cttattatta ctgattcaga ttattaccaa agatttgttt
    10741 ctaaaaagaa aaataaatgg attttagatt attttgaaaa aaatctttca aggattttta
    10801 aagagacaaa tcctgatatt gtaagtgagg tggggctttc ttttaagcta tgcagagaaa
    10861 attctccggc agtgctcaaa aaaattaaga actatttaat tttttctttt gaccaaaaac
    10921 gaggatatat tccccaaaaa gatacacgag aaaataacga tcttcaaaag gcagagcata
    10981 gaaatattat tgccttgatg tttttaaaag attttaaaaa attatacaaa gggccaaatc
    11041 ttataaaatt tacattttaa tttataataa ctgttattgt gagcaaggtt aaaggatgaa
    11101 tgtttatact ttatcatcta ttatttattt ttaattcaaa atcgctcaat attataatat
    11161 gaaactttct tttttaagaa aaaaacatcc tcagtttatt tatcaatcat tttcttatca
    11221 attaaaaaga aataatttaa ttattacttt tgattttaga atccctccag attttaaatt
    11281 ttcaccacaa ttaaaaataa aaaatattga taaaaaacga tttcaaaaaa taaaaaagga
    11341 aaccttagaa aatcttattt ttaatttggg aatggttgaa tctttaagct attggaaggc
    11401 gaccttatca ccaaaaataa atgttgaagc cggttattta acaaaagaac aaatctcttg
    11461 gtggcaggat ctaattttaa aaggaatggg tcagtttttt tacgaaaata aaattgattt
    11521 tacaaaatca aattttctta aaattaaatc ttctcaaaaa tcactaaatt ttgatggtcc
    11581 agaaaaatta caattaaaag ataggatttt gattcctctt ggcggaggaa aagattcaat
    11641 agtaacctta gaaatactaa aagaggcaga agaagaattt aaaatttttt ctttaaaccc
    11701 aaccgaagcg attaaaaaaa taataaaaat aggaaaggag aaatctccta ttgtgattga
    11761 aagaaagata gatcctttac ttttaaaatt aaatcaaaaa ggttatctaa atggtcatac
    11821 gcctttttct gcatatttaa cttttttaac tgctctttgc gcggtgattt ttgattttaa
    11881 atatgttgct ttttctcagg agcgaagttc aaatgaggga aatattaaat atcaaggcag
    11941 gattattaat caccagtatt caaaaagttt tgaatttgaa aaaaagttta gggattatct
    12001 tgaaaaatat ttagtaaaag gtgtttattc ttttagtttt ttacggccgc tttatgaaat
    12061 tcagattgct aaattatttt cttgttatcc tcaatatttt tcagcatttt tgagctgtaa
    12121 tgaagcacaa aaaacttatt ctggcacaaa aaggccaatt caaaaatggt gtggccaatg
    12181 ctccaagtgt ctttttgttt ttactattct ttatcctttt atggaggaaa aaacattaat
    12241 aaaaattttt cataagaatc tttttaaaga tcaaaattta ttacccttaa tgcaagaact
    12301 aattggaata aaagatttca agccctttga gtgtgtaggc acaaaaaagg aaagtaagat
    12361 tgctttttat ctagcttgga aaaagaaaaa aaagagtggg ggagaattac cgtttttact
    12421 gatgtggttt gaaagaaata tttttccaag agagaaaaat tgggaaaata tttcaaaaga
    12481 aataatgcag ggatttgatc aaaaccattt tttgccaaag gagtttgaga gaatattaaa
    12541 gaaaaaatta aaaaaaccag cacaaaccct tgacaaattt tgaattaggg aatataattt
    12601 tattgaaggt aataaaattg cctttttgaa tatttgtact atctccaaaa cagaatcttc
    12661 tgaaggatca aaaggttgta aaccagtaag aaaagggaga tttaaaagac tgcttttaag
    12721 agaagcagta gttttagttt ggcttttttt aatgtttttt gtcttgccgg aacaaatagt
    12781 attagctgaa actggttctg taaaaatgtc tctttctaat atagaagtgg cacaacaaac
    12841 aaaaactaat ttagaaaaaa atagtagtgc cattcaatcc ttagaagagc attttttaag
    12901 taatcaagaa gaagggatag taattactcc aaatactcct gtgataaaaa accttatcca
    12961 ggatcaagaa ttattaaaaa aagaaaataa agaacaagga caggaacaag aacagcgaaa
    13021 agaaagtgtt gaatatacaa taaaagaagg tgatactatc tctggtattg ccgcatcctt
    13081 tggattaaaa acccaaacta ttttatgggc aaataatctt aatgactcaa gtatcataag
    13141 gccaggagat tcaattacta ttccacctgc tgatggtatt atttatactg tgaaaaaagg
    13201 agatacatta ggtaaaattg ctcaagatta taaatctgat ttaaatgaaa ttttagattg
    13261 gaatcaatta gataacgata agattgttga gggtcagaaa ctgtttttgc caggcgctaa
    13321 aaaaccggtt attgtagtaa aaacttcccc tgtctcttcc tcttcttctc cagtgacttc
    13381 aaatgttgtt cctcgtaaag caccagttta ttcttcttgt catcaatttc catatggtta
    13441 ttgtacttgg tatgtagcac aaaaaagatg tgtgccttgg ggaggaaacg ccaaagattg
    13501 gccgtcaaat gctcaggcat atggttatag tgtttgttgg ggaagtaatt gtgattgtcc
    13561 agcgggtagt attttagtaa ctagagagag ttggtatgga cacgtatctt atgttattgg
    13621 tcaaacaaat accactatta ctatttcaga aatgaataga tatggttggg caaaagtaaa
    13681 tactagaacc attaacaagg gcgattggat tattagggct tgtatatact aaatcgatgt
    13741 ctaacttgct aaaattagta atggaaggat tgatggttgg ggttgctatt tttacaccgc
    13801 tttatattga tcctcattct accgaaggat tgagtgagtt gaatccatgt tatgctgaag
    13861 gaattgatga gatggacaaa gaagaaattt taattattga tgaaggatta gaatacaaga
    13921 ttgaaaagaa tggtggaaca atagaagaaa ttttaaagaa cgctcaaatt gaaattaacg
    13981 aggaagatat tattttcccc gcaaaagaga ttgtttttca atcagggaca aatattatta
    14041 ttcaacgcgc tacgccagta attttaaatt tttacggcaa agaacaaaaa atttttaccc
    14101 aaaaggaaaa aatttcagaa gttttgattg aaaaaggaat agaaataaaa gaaaatgatt
    14161 taataaatgt tgatcttgaa aaatcaattt ttccaggaat agaaattaaa atttggcaga
    14221 aaccaaagcc aaaagaaccg gagatagcaa agacaggcag atcgcagaca ggtattggta
    14281 gttggtataa ttttattcca ggagattttt gtgcctcaac tgcttttaaa aagggaacta
    14341 aattattggt taccaatctt gagaacgggc gtcaattagt ggttacagta aatgattatg
    14401 gtcctgttaa ttcaagaatt attgatcttg aaaaaactgc ttttgcaaaa cttgctcctc
    14461 cttcaaaagg agtaataaag gttaaggtag aggaaattca tcattaagaa aatttttaaa
    14521 tttttaaaaa cgcccttctg gcgttttttt agattaaaat aaaacctttc ggagtgccgg
    14581 gaattgaacc cgggtcgcac ccaccccaag ggtgtatact accattgtac tacactccgt
    14641 taaataaaaa tattttattt aacctttgaa agagttttaa tacattgagt acaggctttt
    14701 actctttttc cattagaaag atgcacccat tgaaggttgg gatattttct ttttttaggg
    14761 gcaggattgt acttgccacg aagcttattt agaggtatac tctttatata agttttaccg
    14821 caaattgcac actttcgcat aatcaacgaa tgtacgaatc tatattaata tacgaatttg
    14881 taaattcgta atgaattcgc aattcttaaa cattatactt tatttttctt ttttttgcaa
    14941 tattttatgg atgtttttat tttaattttt atttttttaa tattttattt ctcaatgact
    15001 gttcatgaag ttgctcatgg ggcagtagcg ggaatgttgg gagatcctac tgctaaattg
    15061 gcaggacggc ttactttaaa tcctttgaaa catcttgacc ctattggatc ggtaatttta
    15121 cctgcatttt tgattttaac aagtgctttt aacgggggaa gtgggatcat ttttggatgg
    15181 gcaaaaccag ttccctataa tcccaataat ctctacgata aaaaatatgg gagagcaaaa
    15241 gtaggtgcgg ctggccccct tgctaatttg cttgttgcta ttgtttttgg tctctgtctt
    15301 cgttttttgc cgatgacgga aaatgccttt tttcaaaatt tacagatggg atttatttat
    15361 attgtatgga taaatttact cttctttgtt tttaatttat ggccagctcc tccctttgat
    15421 ggtcatcaca ttctttttag tttttttccg tcattggaaa caaaaattgg ctttttaaag
    15481 aatccattct tttcaatgtt aatggcaatt ttatttatgt ctattattgg ttttccttat
    15541 atctgccctt ggttgtttca attattaaca ggaatgaata tattttaaaa taagtgtcat
    15601 tttgattttt gcttttttaa ttaaattcca tgtcccaaat tgatgaaata aaagagcggt
    15661 tagatatcgt acaggtagtt tctgaatatg tgcagcttaa aaaagtagga gcaaattatc
    15721 gggctctttg tccttttcat tctgaaaaaa caccttcttt ttatgtttca ccctcaaaac
    15781 aaatttataa atgctttggc tgctttctgc ccactacctt ggtcaaaact gaaaatggat
    15841 atcaccccat tagtggaatt caaacaggcc aaaaagttct tactcacaga ggcagatata
    15901 tgccagctat taagacattg agagactacg aaggagaagt gattgattta aaattaagga
    15961 aatcaaatga agttgtttct ttgaccttgg atcacgaagt gtacgttatt aaaacaaggc
    16021 attgtaagta taaaaataga ttgacaagaa tttgtaattg gaattgtaaa aaaagttgcc
    16081 cagcaaaatt ttatcaaaat tacaaaattg aaaaattacc agctagtcaa ctatcgatag
    16141 gtgattattt actttatcca attaatcaag agattagaga catagagttt cttaatttag
    16201 aaaagtattc tacatggaga aaaggtgttt ttggatttaa tccaaaaaca attcctctga
    16261 agataaaagt tgataaaaat ttcttaaaat taattggtta ttatatagct gagggaagta
    16321 atcatcgagc atatattaga tttagcttag gaagccatga agaattattt gcaaaagaga
    16381 taagggagtt gattgaagat atatttaaaa ttaaaacaac aacttataaa cggaaaaaag
    16441 aaggtaaaac aggattggaa attactgctt gtaattcaaa attggctaat atttttggga
    16501 atctttgtgg taaaggagca gcaaataaac atattccttt cgagtttcaa taccttcctt
    16561 cggaaaagca aaaaacaatt ttagaggcta ttcataaagg agatggttgg acaaaagaag
    16621 gaaagattaa gtatatcgat acaatttctt taatactagc ggaacaaatt agagatattt
    16681 tattgagatt aaacattgcg ccgacttttt ggattcatca aaagaaaatt gacaaaaaag
    16741 gtactcatca taaaaaaagt tttaccgtaa tgtggcaaga acattatcag ctaaattatt
    16801 ctcaatttta caacgaacct ggcgggacag aatattggct tttaccaatc gaaaatatca
    16861 agaagaaaag ttataaagga aatgtttata atttagttgt agataaagat cattcttata
    16921 caacttctaa ttttgtagta gggaattgtg gcaaatcagg ggatattttt aaatttataa
    16981 tggatattga aggggttgag tttaaagagg ccttagagat tttagcaaaa aaagctggag
    17041 tagaactgaa aaaggaatca cctcaaaaaa gaactgaaag acaacgtcaa tatgaaatat
    17101 gtgaaatagc aaccaagttt tttgaggctc aaatgacgcg gaccaatgca ggaaaaaagg
    17161 caaaagatta tcttttgtca agaggaattt ctgaaaaatc aataaaagaa tggcggttgg
    17221 gttatgcgcc taatagctgg caggggcttt ctaatttttt agtaggaagg ggatataaaa
    17281 gagaagagat tgtaaaagca gggctggcag tgcagagaga aaatattagc ggaaattcgc
    17341 aagacattag caattattct cgaggctcat atgaccgttt tcgggggaga ataatgtttc
    17401 ctatctttga tattcaaggc aatgttattg gttttggcgg tcgaatcaca gaggagcaaa
    17461 atgaaaaaat gaaaaaagaa aaaggaggag caccggcaaa atatatcaat acgtccaata
    17521 ctttaattta tgataagtca agagttcttt acggattgca caaggcgaaa acatcaattc
    17581 gagaaaaaga tgaaattgtt ttagttgaag gatatacaga tgttatttta agttcacaag
    17641 ccggagcaaa aaatgttgtt tcaacatcag gcactgccct gactcctttg caattaaaaa
    17701 ttctttcccg ttatagtaaa aatcttactt tatgttttga tatggatatt gctggtcaag
    17761 ctgctaccaa aaaaggtatt gacattgctc aaattcaggg ttttaatata aagatagtat
    17821 cattgcctga tgaaaaagat ccggccgatg ttataaaaga aaatccccaa gagtggttaa
    17881 atcttataaa agaatcaaaa gaaattataa gcttctattt tgaaaagtgt ctttcgcatt
    17941 ttgacccggg aaaaattgaa gacaagaaaa agattgctga aacactagtg ccccttattt
    18001 caagaattca aaataaaatt gaacaaggat attggattca aaagctagca aatgctataa
    18061 atttaaacga agaagttgtt tggggagaag tcaaaagaat tattagtact catcaatttc
    18121 cttcaaattc ttctaacaat agtaacgaaa ttggtgattc atttgcaaaa attagcgaac
    18181 aaaaaaaaga tcagcaaaga cagatcgagg aacgtttatt aatgatgttt ttacaaacac
    18241 cagaagaaac aaaaaatatt ggaccgatta aattttcttt tcctgagggt caaataattt
    18301 ttaatgcctt gaaagaaaag aaaaaaattc caaaggaatt agaagagctt gtagcagaac
    18361 ttgaattgca atttgatatt gaaaaagaga aagactcaac aatggacgta aaaaaagaaa
    18421 ttgaattttg tattttaaga ttaaaagagt tttcaaggca aaaaaaattg caaagaatta
    18481 ttaaagaaat aaaagaagcc gaatctaaaa ataacaaaga aaaagtttct aagcttttag
    18541 aaaaattgaa caaaatataa aataaatatg aaaaagcaaa ctaaaaaaac aaaaaagaat
    18601 aaaacatctt taccatcttc caaaaccaag aataaaaaca aaaagacaaa aaggacaaaa
    18661 cctctttctc aaaataaatt gaaaaataaa acaacaacta cagaaagcaa agcaaaaaaa
    18721 ttgcaaagca aaaaaaaatc agaggcattc tcaaggagta gaatcgctat aaaggaagag
    18781 gcattaaaag aattaataaa gagaggaaaa tctcgtggtt ttgtgactct ttcagaagtt
    18841 ttaaaatttt ttcccagagc agaagaagac atagctgggc ttgaagaaat ttatcaaaaa
    18901 ttagaaagcg aggggttgag agtagaggat tctgttgatt ttttgggaga catagatctt
    18961 gcttcttctc ctgaacagat tccagagata gactctattc aaaaatattt aaaagaaatt
    19021 ggccgttatc cgcttttgac tcttgaagaa gaaaaagagt tatctcaacg tttagaaagg
    19081 ggagacaagg cagctcagaa aaaattaatt caatcaaatc ttcgacttgt ggtctccatt
    19141 gctaaaaaat atcttggccg gactcctcat ttaacattgc ttgatttgat tcaagaggga
    19201 aatattgggc ttttcaaagc agtggaaaaa tacgactggc ggaagggatt tcgattttct
    19261 acatatgcta cttggtggat tcgtcagtcc attacaagag ctattgctga tcaatctaga
    19321 acagtgcgtt taccagtgca tattgtagaa cgactttcta aatataatca gataaaaaaa
    19381 cgacttttgc aagaattggg aagggaacct ttagcagaag agattgctat tgagatgcac
    19441 gaggatgtta aaaaagtaaa aaatcttgag aaggtggcaa tgggtactgt ttctttagag
    19501 caaccaattg gggacgacga taaagaaggg acgcttgaag aatttattga agataagaaa
    19561 actgcttctc ctgctgagac aacgagtctt tctattttaa gggaccagat aaaagaaatc
    19621 ttaagcgatc ttgcacccag ggaacagcaa attttaaaaa tgagatttgg tattgacgga
    19681 gaaatacctc ataccttaga agaggtagga aaagaattta atgttacacg agaaagagtt
    19741 cgtcagattc aacaacgagc tattgagaaa ataaaagagc ataaaaaagt aaaaga
//
LOCUS       DAOTQD010000006        19207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_2912_length_19207_cov_7.530702, whole genome shotgun sequence.
ACCESSION   DAOTQD010000006 DAOTQD010000000
VERSION     DAOTQD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 19207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19207
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_19207_cov_7.530702"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1016)
                     /locus_tag="P5570_00525"
     CDS             complement(12..1016)
                     /locus_tag="P5570_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRR39234.1"
                     /translation="MARIGIDLGTCNSIVFLQGKGVVLSEPTVVAVSQGENRVLAIGK
                     EAKEMIGRTPDTIIAYRPLRDGVIADFRVTQAMLRYFIKKVQGTFSFFKPEVLIGIPV
                     GATSTERRAVIEAALKAGAKEAFVAKEPVLSAIGAGVPINSPSGNMIVDIGGGTTEVA
                     VISLGGVVSASSVRVGGDRLDEAISDHIKEKYSLAIGEQTAERIKIQIGSALPPEEEE
                     KMEIRGRDLFSGLPKIIEISSSEICQAIQAELEEMLQCVKSVLKETPPEVSADIIDKG
                     MVLAGGGSQLRRIDEYFKMHTGVSTQIADEPLFCVAKGTGMVLEHLDAYKKSIMTKKG
                     "
     gene            complement(1176..2849)
                     /gene="murJ"
                     /locus_tag="P5570_00530"
     CDS             complement(1176..2849)
                     /gene="murJ"
                     /locus_tag="P5570_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRR39235.1"
                     /translation="MSFFNHRAKNVTLAAFILMVTTLIAKLLSLARLRVFTTLFTTEE
                     LDIYFAAFRIPDLLYNVLILGAISTAFIPVFSDYWSKSKEEAWKLCNNVLCVFLGLLI
                     ILAILLAIFAPQLMHLVAPGFDDAKMNLLIRLTRIMFLSPIILGISNIFGSLLQYFSR
                     FLIYSLAPIMYNVGIILGALFFAPKMGIEGLAWGVVLGAVFHLCVQLPAVFLSGYRFN
                     AVFDFSHQGLRKIFKLMVPRTIGLAAQQINLIIMTAIASLLAVGSITIFNISNDLQYI
                     PISLFGVSFATAVFPSLSRSFSRAKASLNKAKKEKEKFMQKFISVFSQILFFTLPLSI
                     LFFLLRAQLVRIIPGSENYTWQDTRLTAACLGIFAFSIFAQSLIPLITRAFYSFQDTK
                     TPVKISVFSIILNIIFAFLFVYIFSFNNGLYYFFQSFLKLEGISQISIIGLPLAFSLS
                     SVINLILLLSAFKKRVGDHWDLKLGNVFLRILFLSLLCGGVTFGCLHLFALFFDLQTF
                     GRIFLQAVFAGGIGLIFYIYLSKILHFPEYDLIFEPFLARKKYGNKEKK"
     gene            complement(3020..4549)
                     /gene="rny"
                     /locus_tag="P5570_00535"
     CDS             complement(3020..4549)
                     /gene="rny"
                     /locus_tag="P5570_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRR39236.1"
                     /translation="MTEYILLILGAASLGVGAILGYFTRQSVAKFQAGSIERTIQEKL
                     SEAKTEAQNIILDAKKKAFSLSEQTRKKIEDRERELMVLERQLERRQGILDQKIADFE
                     EKEKNLLREKRELARLRGEIDKIISQKKQELERISGISKEEAKKEIFAETQKEYQKEF
                     EQKLSRLEKEGEEKIQQRAKEVLSFAVQRLPSSSITELTTTIVSLPDDEIKGRIIGKE
                     GRNIKAFEEATGVELMVDETPNVVFLSSFDPLRREIAKIALERLIKDGRIQPSRIEEI
                     VKDVQEKIPEKIQKIGKEAAYKVEVFDLDPKLHQLLGRLKFRTSFGQNVLDHSIEVSI
                     LAKSLAEEIGADSKIAAKAGLLHDIGKAVDWQVEGSHIDIGIRILEKFKVENEVIKAM
                     KSHHEDYPYESIEAVLVQTADAISASRPGARKDNTENYLKRLEELENIAKRFPGVKNA
                     YALEAGREIRVFVKNDEVDDFESKKLAQEIAKNIEEELNYPGEVKVVVIRETRVTEYA
                     R"
     gene            4870..6447
                     /gene="gpmI"
                     /locus_tag="P5570_00540"
     CDS             4870..6447
                     /gene="gpmI"
                     /locus_tag="P5570_00540"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRR39237.1"
                     /translation="MKKVILIVLDGWGIAPPQPGNAPSQANIPAIKNIEEYYPATILH
                     ASGINVGLPWWEEGNSEVGHLTMGCGQIIYQYLPRIITAIQNGSFFENPALIETINYV
                     KEKNSRLHLMGLVSSGAIHSYIDHLYALLDLCKKNNIEKVFLHVFTDGRDTPQKEAVI
                     FLQNLNQRLQQGKLGEIATVCGRSFAMDRDENWDRIKKVYQCLTEGEGKHISNLTQYL
                     ENSYQMGLTDEYIEPAVLNPEGIIKKNDGVIFFNFREDRAKELTRAFVEENFKGFERK
                     IRFNIGGEKPDLCFATMTKYLENNPYIKVAFEPPKIENTLGKVISDAGFFQLRIAETE
                     KYAHVTYFFNGLKEKPFPGEDRVIIPSVGGPHYDKNPEMRALTITEKVIEEMKKEKYQ
                     FILVNFANADMVGHTGNLKAAIKAAEILDGCVKKITEESKNKYVTMITADHGNFEEMI
                     NQKTGEIIGEHSSNPVPFYLVDLNLRSEFPQKKELEFQGEAGGFLFDIAPTILEYLEI
                     KKPNEMTGISLLSTLLR"
     gene            complement(6421..7728)
                     /gene="tig"
                     /locus_tag="P5570_00545"
     CDS             complement(6421..7728)
                     /gene="tig"
                     /locus_tag="P5570_00545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRR39238.1"
                     /translation="MEVQIENLPKSKVKIKLRLTGEEFSPYLLKATENLAKTLNLPGF
                     RPGKVPAEIAKEKLNENDILEEAANIALNDSFPKIVEEKGLKVLGRPQARIEKISRQE
                     FEASIETSLFPNIELPDWKKIAKEEAQKEIKIEDKEVEDALSYLQKSRAKYVKVFEPA
                     SKGKQVEVDYEIRSGGVKIENGDIRGQKFILGENSKENGESIFIPGFEEKILGMRENE
                     EKEFSLMAPSSFWQKELQGKPLDFKVRMKGVFKVDTPEINDEFAQSLGKFKNKEDLQK
                     NISEGILEEKRQEEKIRWQSAVLGKIAKEAKIDLPDILIEEERDAMIDDLKKRIEQMG
                     ISFEQYLLHLKKSIDDLKKELLPEAEKRTRMLLCIYEIAKKENIKIEEKEIEEEINKI
                     LKQHPEMAEEIKSQEETLKSYLREQILQKKVVDLLLSQQSGEK"
     gene            complement(7803..8381)
                     /gene="clpP"
                     /locus_tag="P5570_00550"
     CDS             complement(7803..8381)
                     /gene="clpP"
                     /locus_tag="P5570_00550"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRR39239.1"
                     /translation="MNLIPTVIEKSPEGERAYDIYSRLLKERIIFLGGQITDDVANAV
                     IAQLLFLESQDSKRDIKLYLNSPGGSVTAGLAIYDTMEYIKCDVSTICVGMAASMAAV
                     LLAAGQKGKRFALPNSEIMIHQVMGEAGGQAVEIEISAKHILKIRDRINKILSKHTGQ
                     PLKKIEKDTDRDFFLTPEEAKAYGIVDKIIKK"
     gene            complement(8542..9618)
                     /gene="ychF"
                     /locus_tag="P5570_00555"
     CDS             complement(8542..9618)
                     /gene="ychF"
                     /locus_tag="P5570_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010648886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRR39240.1"
                     /translation="MSLSIGIVGLPNVGKSTLFQTLTKKQVDRANYPFCTIDPNVGVV
                     AVPDVRVDKLAELTKSKKKIYATVDFIDIAGLVKGASRGEGLGNQFLANIREVDAILY
                     VLRAFSDNKIINTQDSINPFNDKEILDTELILKDLETVEKRLKSLEGDIRRGDKEAIK
                     ENETLLKAKKILEEGKVLFEFKEKEFKEKEFEELKNYQLLTAKPRLYLLNGKDEEVPQ
                     DVIDEFKNHNWPFLIIDLLTEFEAAGLSSKERKELELPEKTELDLLIKKSYEILGLIT
                     FFTTGEDETRAWTIKKGTKAPQAGGVIHSDFEKNFIKAEVINWEDLIKAGGFAAAREK
                     GLIRTEGKDYIVQDGDVIEIKHNA"
     gene            complement(10228..10316)
                     /locus_tag="P5570_00560"
     tRNA            complement(10228..10316)
                     /locus_tag="P5570_00560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10280..10282),aa:Ser,seq:gga)
     gene            10736..11737
                     /locus_tag="P5570_00565"
     CDS             10736..11737
                     /locus_tag="P5570_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRR39241.1"
                     /translation="MFKLQRYSKNPILTPIKEHPWESKMVFNPAAVLKDNKVHLIYRA
                     MGEDEIDGYPISRLGACVLKNDGVTIEKRYEKPILEPKKWYEPAGCEDPRITEIDGKY
                     YLLYTAYLGYNAPPLFKEEKTNIAMAVSDDLFHWKRFDHLLLPDVFEPEKNGVLFPKK
                     INGYYVLFYRIEPSIYVAYTKNLTPFPPKWIGHKVIATPRENSWDSWKIGAGAPPIET
                     KDGWLLIYHGIDRRGVSRREKKKKDGKKEERIYRLGVMLLDKEDPEKILYRSKNWILE
                     PEEPYEKEGAVPNVVFTCGAVVIKDTLFVYYGGADTVIGVATCSLKELLSAIKKGEV"
     gene            11752..12624
                     /locus_tag="P5570_00570"
     CDS             11752..12624
                     /locus_tag="P5570_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HRR39242.1"
                     /translation="MLSAKKYKGNPIIVPSLEKKESSIYNPTTVVRKEKVFLLGRSEF
                     GYENKTISKINLFSSQNGFSFKPFPKNPVIKPEKKYEKMGCEDPRVIKTDKNNYFLTY
                     TAYKGKDKNGDYKIKLMGALSKDLISWQKFPLVDNEKSGAVVQNYKHKDQYVMYFGGK
                     EIKIAFSRDLKKWKFNAKPIFTARKGNFFDNYLVEPGPPPILIKKGILLIYNGKNAAG
                     KFSAGWAIFDKNNPAKLLERSRKPILEPTEYWEKYGKVNNVIFATGLVFFKGKWLLYY
                     GGADKSTGVAVLNF"
     gene            12661..13845
                     /locus_tag="P5570_00575"
     CDS             12661..13845
                     /locus_tag="P5570_00575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR39243.1"
                     /translation="MNKNSLGICIVSSYSPRKCGIATYTSSLKQNILKIAPDTNIKIV
                     AINDPQKKYHYSKRVIATISQEKKETYIKAANAINKNKEIDVVNLQHVFSLFGGKNGE
                     FIIDFLKHLKKPVVTTMHMIYSALEKPNSLEVVDKSYKTITEKIISLSSKIIVIIQPM
                     ADILESQYHCSSEKIAVIPHGVPEVKKENPKKYKKLIGFENKKIISTFGLIRPKKGLE
                     YLIWAMPEIVKKYPEAIALILGESHPNRPKEYYNFLKEEAKKTGLLDKNIYFNSHYLK
                     FKEIITYLLATDVFITPYLVPEQTSSGVIAYALGCGKVVVSTPFLYAKEMLKDGRGIF
                     INFKDSQSIFSAIDFIFSHPEERKKIERKAYITAQKMLWSKVSKEYLKVFKDGIKNYE
                     KK"
     gene            13832..15004
                     /locus_tag="P5570_00580"
     CDS             13832..15004
                     /locus_tag="P5570_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR39244.1"
                     /translation="MKKNDLKVLYLSSFPPRQCGIADFTQNTINAVYKVNPNIDQKVI
                     AINEKNAPKRDYLATVKWELEQEDLDSYQKTANYINECGADVLFLQHEYGLFGGFDGI
                     FVLKLLEKLKVPIVSFYHTIPLIPTSKRREFRLNIIKNIGNISKHIITTTQSGKEILE
                     KECKIPVRKISIVHHGGYDIPYPKQKDKEELKKKYGFQGKFIVLTYGLITKSKGIDYA
                     IEAIAKLKDKHPNILYIIAGTSHPVKIKLAEKDYYSDLQLKTKKAKATNNVKFINKYF
                     EEEELIDYLKMADLFLIPYLVREQISSGVLANAATTGNCILSTPFPYAKEIIGEDEER
                     GYFIDFENSDSIAQKIAFLIENPKIIEKKRKEMYQFGRNFTWQKMAEEFIKILQKK"
     gene            complement(15104..16720)
                     /gene="groL"
                     /locus_tag="P5570_00585"
     CDS             complement(15104..16720)
                     /gene="groL"
                     /locus_tag="P5570_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909804.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRR39245.1"
                     /translation="MAKIIKYSEKAREKLLKGVDKLANAVTITMGPKGRNVVLDKGFG
                     APEITNDGVSIAKEIELEDKFENLGAEIVKEVASKTNDVAGDGTTTATLLAQELIREG
                     FKNVTAGANPLEIKRGIDKGVEKVVQQLKSISKKVSRFEEMAQVATISAEDEKMGKLI
                     AEVFDEVGKDGVVTVEESKTFGLQKEIVKGMQFDQGYVSPYMVTNPDRMEAVYDDPYI
                     LITDKKISSLQEILPLLEKLSQSGCKDLVIIAEEVEGEALATLVINKLRGTFNTLAVK
                     APGFGDRKKEMLQDIAVLTGGQVITEELGLKLENTEINQLGRARRVVSKKENTTIIEG
                     KGKKQDIDARIEQVRKEMETTESEFDREKLQERLAKLSGGVAVIKVGAATEVEQKQKQ
                     QKIEDAVAATRAAIEEGIVPGGGVALVRCIPVLDEVKVVKEQKVGLSILKRVLESPIR
                     RISKNAGVDDGVVVQKVKDGKDGFGFNAKTNQFEDLLAAGIIDPTKVVRCALQNAASA
                     AGTLLTTECVVAEKPEEKKDQMPSPMPEGY"
     gene            complement(16784..17074)
                     /locus_tag="P5570_00590"
     CDS             complement(16784..17074)
                     /locus_tag="P5570_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRR39246.1"
                     /translation="MNIKPLADYILIEPIKKEEKTKSGILLPETAEKEEPQEGTVIAV
                     GPGKKLSSGKILTPDVKKGDKVLFKKYGPDEIKVDDKEYLIAKEEDILAILE"
     gene            complement(17320..17862)
                     /locus_tag="P5570_00595"
     CDS             complement(17320..17862)
                     /locus_tag="P5570_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39247.1"
                     /translation="MKDNDKNPIKNVDEFIKKVDEMKKKDKFDLSSDEDLSIAIMNLV
                     SIEEHFFFTGAKLQKPEYYNMIKDVREMRKELLKKIVKEPEGELWCISKHLLASSMRL
                     MEVGTKQLGKGKKDEAYNLFDKAYDLYSLFWGLNLKLIDVGDVKKIDDKALNKHDKEK
                     KGFLGKLGELVKKAIDCCIE"
     gene            complement(17867..18382)
                     /locus_tag="P5570_00600"
     CDS             complement(17867..18382)
                     /locus_tag="P5570_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39248.1"
                     /translation="MKKTLIFLFSFLVAGFLFGNKALAVCPLCTIAVGAGVGLSRWLG
                     IDDTITGLWIGGLIVSLIIWTDGWLAKKNIHFWGRTVLVVIAYFALVIIPLFYSGIIG
                     NPLNTLCFCHLDKLLFGIIVGSIAFWCGANWHYYLKEKNNGHVYFPFQKVVISILPLI
                     ILSVLYYFLTK"
     gene            complement(18385..18804)
                     /locus_tag="P5570_00605"
     CDS             complement(18385..18804)
                     /locus_tag="P5570_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39249.1"
                     /translation="MDKKIVFSTIVFIAIFIFSIFVLSEENANKIENNETSQQNQEAM
                     QQSMNNVVLFYSDDCPHCKIIEEYIEENNVQEKVSFTRKEVYYNNLNLKELQMRAEDC
                     GLSGDSIGIPFLWDGKNCLMGEQEIIKFFEQKMAEGQ"
     gene            complement(18997..19107)
                     /gene="rrf"
                     /locus_tag="P5570_00610"
     rRNA            complement(18997..19107)
                     /gene="rrf"
                     /locus_tag="P5570_00610"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6523 a         3578 c         2724 g         6382 t
ORIGIN      
        1 gcaggtttga attatccctt ttttgtcatt atgctctttt tgtaagcgtc taaatgctct
       61 aaaaccatac ctgttccttt tgcaacacaa aataatggct catcagcaat ttgagtagaa
      121 acaccggtat gcattttaaa atattcatct attcttcgca attgagaacc gccaccagcc
      181 agcaccattc ctttatcaat aatatcagca gaaacctctg gcggtgtttc ttttagaaca
      241 ctttttacac actgaagcat ttcttccaat tctgcctgaa tcgcctggca aatttcactt
      301 gaagaaatct caataatttt tggaagaccg gaaaataaat ctctgcccct aatttccatt
      361 ttttcttctt cttctggagg caaagcagaa ccaatttgaa ttttaattct ttcagcagtt
      421 tgttcaccaa tcgccaaact atatttttct ttaatatgat cactaattgc ctcgtccaaa
      481 cgatctcctc ccacccttac agaagaagca gaaaccactc cgcccaaaga aattaccgct
      541 acctcagtag ttccgccacc aatatcaaca atcatattgc cagaaggaga atttatagga
      601 actccagcac caatagctga cagaaccggc tcctttgcca caaatgcctc tttggcgccg
      661 gcttttaaag cggcttcaat cactgctctt ctttcggttg atgtggcacc gaccggaata
      721 ccaattaaga cctcgggttt aaaaaaagaa aaagtgccct gaaccttttt tataaaatat
      781 cgcaacattg cctgagtaac cctaaaatca gcaataacgc catctcttaa tggacgataa
      841 gcaataatgg tatcaggagt tctgccaatc atttcctttg cttctttgcc aatagctaaa
      901 accctgtttt ctccttgaga aacagccact actgttggtt cagacagaac cactcccttg
      961 ccctgcaaaa acacaattga attacaagtg ccaagatcaa tgccaattct agccataaaa
     1021 attaaataaa ataaactgcg cttatatcct tttttaatat aacaaaaaaa ttgctaaaaa
     1081 tcaacaaaaa ctatctgaac aatggccata aaagccacaa aatcattgaa agcatcacaa
     1141 agaacaccag cactgaaaaa ataatttgta aaatattatt ttttttcttt gttgccatat
     1201 tttttacgag ccaaaaacgg ctcaaaaatt aaatcgtatt ctggaaaatg taaaatcttt
     1261 gacaaataaa tataaaaaat caatcctatg ccacctgcaa aaaccgcttg taaaaatatc
     1321 ctgccaaagg tttgaagatc gaaaaaaaga gcaaaaagat gcaagcatcc aaaagttacc
     1381 ccgccacata acaatgataa aaataaaatt cttaaaaaga cattgcccaa ttttaaatcc
     1441 caatgatccc ccactctctt tttaaatgca ctcaacaata aaatcaaatt tatcactgac
     1501 gataaagaaa aagccaaagg aaggccaatt attgatattt gagaaattcc ttctaacttt
     1561 aaaaacgact gaaagaaata ataaagacca ttattaaacg aaaaaatata aacaaataaa
     1621 aaggcaaaaa taatattcaa aataattgaa aaaacactga ttttaacagg ggttttggtg
     1681 tcttgaaacg aataaaaagc ccgtgttatt aagggaatca aactttgagc aaaaatagaa
     1741 aaagcaaaaa ttcccagaca agcagctgtt aaacgcgtat cttgccaggt ataattttca
     1801 ctgccaggaa taattctaac aagctgagcc cgcaaaagaa aaaataaaat agaaagcggc
     1861 agggtaaaaa aaagtatctg agaaaaaact gagataaatt tttgcataaa cttttctttt
     1921 tccttttttg ctttatttaa tgaggccttg gctcttgaaa aacttcgcga aagagaagga
     1981 aaaacagccg tagcaaaaga aaccccaaaa agagaaatag gaatatattg aagatcattg
     2041 gaaatattaa aaatagtaat actacccaca gccaacagtg acgcaatggc tgtcattata
     2101 ataaggttta tttgctgggc agcaagccct attgttcggg gcaccatcaa tttaaaaatc
     2161 tttcttaatc cctggtgact aaaatcaaaa actgcattaa aacgataacc agaaagaaaa
     2221 acagccggca attgaacgca aagatgaaat acagccccta aaactacacc ccaagccaat
     2281 ccctcgatac ccatctttgg agcaaaaaac aaagcaccca aaataatacc aacattatac
     2341 attatgggtg ctagagaata aatcaaaaaa cgagaaaaat attgcaataa agaaccaaaa
     2401 atattgctga tccccaaaat aataggcgat aaaaacatta ttctagttag acgaatcagc
     2461 aaattcattt ttgcatcatc aaaccctgga gcaacaagat gcattaattg aggtgcaaaa
     2521 atagctaata atattgccaa aatgattaaa agacccagaa acacgcacag tacattattg
     2581 caaagtttcc atgcttcttc ttttgatttt gaccaataat cagaaaatac cgggataaaa
     2641 gctgtagaaa tagctcctaa aattaaaaca ttatacaaca gatctggaat gcgaaaagca
     2701 gcaaaataga tatcaagctc ttcagtagta aagagtgtag taaaaaccct taatcttgcc
     2761 aaactcaata gtttagcaat aagagttgtt accattaaaa taaaagctgc caaggtaaca
     2821 ttcttggctc gatgattaaa aaaagacata aaaatatgaa tagaatacaa ttcctttctt
     2881 tgcggttggg acaaatttca tacaaaccag ctgttctctt tttttctttt tctctaaaat
     2941 gccttggcaa gaaaaagaag cagaaatttc aaatgaattt tatgagcgac agcggacaaa
     3001 tttctataaa tattttaatt tatcttgcgt attctgttac tcttgtttcc cttatcacca
     3061 ctaccttcac ctcgccagga taatttaatt cttcctcaat attctttgca atttcctgag
     3121 ctaatttttt agattcaaaa tcgtcaactt catcattttt gacaaatacc ctaatctccc
     3181 taccagcttc aagggcataa gcatttttta cccctggaaa acgtttagca atattttcca
     3241 attcctctaa acgttttaaa taattttcag tgttgtcttt gcgagcgccg ggtcttgaag
     3301 cagaaattgc atcagcagtt tgaacaagca ctgcttcaat actttcataa ggataatctt
     3361 catgatgact cttcattgct tttatcactt cattttctac cttaaatttt tctaagatcc
     3421 gtattccaat atcaatatga gaaccctcaa cttgccaatc aacggctttt ccaatgtcgt
     3481 gcaaaagtcc tgctttagct gctatttttg aatcagcgcc tatttcttcc gctaagcttt
     3541 ttgccaagat agacacctct atactatgat ccaaaacatt ttgaccaaaa ctagttctaa
     3601 attttaatct gcctaaaagt tggtgaagtt ttggatcaag atcaaaaacc tcaactttat
     3661 aagcagcttc tttaccaatt ttttgaattt tttctggtat tttttcctgt acgtctttga
     3721 caatctcttc tattcttgac ggttgtattc taccgtcttt tataagccgt tcaagagcaa
     3781 ttttagcaat ttctcgacgt aatggatcaa aagatgagag aaaaacaaca tttggcgttt
     3841 cgtctaccat tagctcaaca ccagttgctt cttcaaacgc cttgatgttt cttccttctt
     3901 taccaataat tcttcctttt atttcatcat caggcaaaga aacaatagtt gtggttagct
     3961 ctgtaatgga tgaagacggc aatcgttgaa cagcaaaact caatacttct tttgctcttt
     4021 gttgaatttt ttcctcgcct tctttttcca atcgtgaaag tttttgttca aattcttttt
     4081 gatattcttt ttgggtttca gcaaatatct ctttttttgc ctcttctttt gaaatgccgc
     4141 ttattctttc cagttcttgc tttttttgag aaataatctt atcaatttct cctctcagtc
     4201 ttgccaattc tcttttttct cttaaaagat ttttctcttt ctcttcaaaa tcagcaattt
     4261 tctgatccaa aataccttgt ctcctctcta attgcctttc caaaaccatt aattctcttt
     4321 ctctgtcttc tatctttttt cgtgtttgct cagacagaga aaaggccttc tttttagcat
     4381 caagaataat attttgggcc tctgtctttg cctcagataa tttttcctga atggtccttt
     4441 caattgagcc tgcttgaaac ttagcaacgc tctgacgggt aaaataacca agaatagccc
     4501 caacaccaag cgaagctgct cctaaaatta aaagtatgta ttctgtcata aaacataaaa
     4561 tgattaataa ttaactagct aatttctaat tgacttaatt aagccccaac gactaatcta
     4621 aaatgactaa aataaactgg attttagata attaggactt atttagacat tagattaact
     4681 ttgacattta acattattat actatcacat tgacattttt ttgtcaaaaa agtaaaataa
     4741 agtattcaaa agtattaatt tacctgcgag cccgctcgtc ttattgaaca tatttgaata
     4801 ttgatatccg tagcaattta tgaattaagt tttcctttcg cttgcgcgcg gatatttgaa
     4861 tcacatttta tgaaaaaagt tattcttatt gtactagatg gttggggtat agcgccccct
     4921 caaccaggaa atgccccatc gcaagccaat atacctgcga ttaaaaacat tgaggaatat
     4981 tatccagcaa ccattttgca cgcttcagga attaatgttg gactcccctg gtgggaagaa
     5041 ggaaacagcg aagtaggcca tttaacaatg gggtgcggac agatcattta tcagtatctg
     5101 ccaagaatta ttactgccat tcaaaatggt tctttttttg aaaatcccgc tttgattgaa
     5161 actattaact atgtaaagga aaaaaactcc cgactgcatt taatggggtt agtttcttca
     5221 ggcgcaattc atagctatat tgatcatttg tatgccctgc tcgatctttg caaaaaaaac
     5281 aatatagaaa aagtgttttt gcatgttttt acagacggaa gagatactcc ccaaaaagaa
     5341 gcggtgattt ttttgcaaaa cctcaatcag cgacttcagc aaggcaagct gggagagatt
     5401 gctactgttt gtggcaggtc ctttgcaatg gatagagatg aaaattggga tagaattaaa
     5461 aaagtttatc aatgtcttac cgaaggagag ggaaagcata tttctaatct tactcagtat
     5521 cttgaaaact cttaccaaat gggccttact gatgaatata ttgaaccagc cgtacttaat
     5581 ccagaaggaa ttatcaaaaa aaatgatggt gtaatattct ttaattttag agaagatcga
     5641 gcaaaagagt taacaagggc atttgtagaa gaaaatttta agggctttga aagaaaaata
     5701 agatttaata ttggcggtga aaaacctgac ttgtgttttg ccacaatgac aaaatatcta
     5761 gaaaataatc cctatattaa agtggctttt gaacccccaa aaatagaaaa cactctgggc
     5821 aaggttattt cagacgctgg attttttcaa ttaagaattg ctgaaacaga aaaatatgct
     5881 catgtcactt atttttttaa cggtttaaaa gaaaaaccct ttcctggtga ggatagggtt
     5941 attattcctt cagtgggagg accacattat gataaaaatc cagaaatgcg agctctcact
     6001 attactgaaa aagtaataga agaaatgaag aaagaaaaat atcaatttat attagtaaat
     6061 tttgccaatg ctgatatggt gggacatacc ggaaatttaa aagcagccat taaggcagca
     6121 gaaatattag atggttgtgt taaaaaaata actgaagagt cgaaaaataa atatgtaaca
     6181 atgatcaccg ctgaccatgg caattttgaa gagatgatca atcaaaaaac aggagaaatc
     6241 attggcgaac atagttctaa tcctgtacct ttttacttag tagatttgaa tcttagaagc
     6301 gaatttcctc agaaaaagga attggaattt caaggagagg ccggtggatt tctttttgat
     6361 attgctccca ctattcttga atacttagaa attaaaaaac caaacgaaat gactggtatt
     6421 tcacttctct ccactttgtt gagataatag aagatcaacc accttttttt gaagaatttg
     6481 ttctcttaaa taacttttta atgtttcttc ttgagatttt atttcctctg ccatttcagg
     6541 atgttgtttc aaaattttat taatttcctc ctctatctcc ttttcttcta ttttaatatt
     6601 ttccttcttt gcaatttcat aaatgcacaa aagcattctt gttctctttt ctgcttcagg
     6661 taaaagttct tttttcaaat catctattga ttttttaaga tgaagaagat attgttcaaa
     6721 ggatattccc atttgctcga ttcttttttt caaatcatca atcattgcat ctctttcttc
     6781 ttctattaaa atatcaggta aatcaatctt ggcttctttt gcaattttac ccaatactgc
     6841 actctgccat cgaatttttt cttcttgcct tttttcttct aaaattccct cagaaatatt
     6901 tttttgaaga tcttctttgt ttttaaattt tcccaatgat tgagcaaact catcgtttat
     6961 ctctggtgta tctactttaa aaacaccctt cattctcact ttaaaatcca aaggtttgcc
     7021 ctgtaattct ttttgccaaa aacttgatgg agccattaat gaaaattctt tttcctcatt
     7081 ctccctcatt cctaaaattt tttcttcaaa tcctggaata aatattgact ctccattctc
     7141 tttagaattt tctcccaaga taaatttttg acctctaata tctccatttt caatttttac
     7201 tcctccgctt cttatctcat aatctacttc tacctgtttt ccctttgaag caggctcaaa
     7261 aaccttaaca tactttgctc ttgatttttg taagtaagaa agagcatctt caacctcttt
     7321 atcctctatt tttatttctt tttgagcctc ttcctttgca atctttttcc aatcaggaag
     7381 ttctatattg gggaataaag aagtttcaat agacgcctca aattcctgcc gagaaatttt
     7441 ttcaattctt gcttggggcc tacccaacac ttttaatcct ttttcctcta ctattttagg
     7501 aaaggaatca ttcaaagcaa tattagcagc ttcctctaaa atatcatttt catttaattt
     7561 ctctttggca atctctgcag gaactttgcc cggacgaaaa cctggcaaat ttaatgtttt
     7621 tgctagattc tcagtagctt ttagtaaata aggcgagaat tcctcgcctg ttaatcttaa
     7681 tttaattttt acttttgatt ttggtagatt ttcaatttgt acttccataa taaattttat
     7741 tcttatttaa ataaatttaa aattgcgcgt atcggtttta aagtttataa aaacaacacg
     7801 ttttattttt taatgatctt atctactatg ccataagcct ttgcttcctc tggcgtaaga
     7861 aaaaaatctc tatcggtatc tttttcaatt ttttttaatg gctgacccgt atgttttgat
     7921 aaaatcttgt ttattctgtc cctaatctta agaatgtgtt ttgctgaaat ttcaatttct
     7981 actgcctgtc cacccgcttc ccccatcact tgatgaatca taatttctga attaggcaaa
     8041 gcaaatcttt tacctttttg accagcagct aaaagaactg ctgccattga tgctgccatc
     8101 cccacacaaa ttgttgaaac atcacacttg atatattcca ttgtatcgta aatggctaat
     8161 cctgccgtaa ctgagccacc cggactattt aaataaagtt taatatccct tttagaatcc
     8221 tgactttcca aaaacaataa ttgagcaatc actgcattag ccacatcatc ggtaatttga
     8281 ccacctaaaa aaataatccg ctcctttaaa agtcgggaat aaatatcgta tgcgcgttct
     8341 ccttcaggag atttttctat aaccgttgga attagattca tattttttaa aaattaatta
     8401 tataatttat ataaagcagt ttatatatta tcttttttaa ataaaaaagt caaatttttt
     8461 gcaatatcaa caaaatttat aaaaataccc cccgtcgaga cgattaagag aattaaaacg
     8521 gaaaaaaatg taatttaatt tttaagcatt gtgctttatc tcaataacat ctccgtcttg
     8581 gactatataa tctttccctt cagtgcgaat taatcccttt tctcttgccg cggcaaatcc
     8641 accggctttg attaaatctt cccaattaat tacctcggct ttgataaaat tcttctcaaa
     8701 atcagaatga ataacaccac cggcttgagg cgcttttgtt ccttttttta ttgtccaagc
     8761 acgtgtctca tcttctccag tagtaaaaaa ggtaatcagg cccaaaattt cataagattt
     8821 tttaattaaa agatcaagtt ctgttttttc aggtaattct aattcttttc tttctttgct
     8881 ggacaaaccc gctgcttcaa actctgttaa aagatctatt attaaaaaag gccaattgtg
     8941 atttttaaat tcatcaatca catcttgagg aacttcttcg tctttaccgt ttaaaagata
     9001 aagtcgcggt tttgcagtta acagttgata gttttttaac tcttcaaatt ccttttcttt
     9061 aaactctttt tctttgaatt caaaaagaac cttgccttct tccaaaattt tttttgcttt
     9121 tagaagtgtt tcattttcct ttattgcttc tttgtctcct ctccttatgt ctccttctaa
     9181 actttttaac cttttttcta ctgtctcaag gtcttttaaa atcaactcag tgtccaaaat
     9241 ttctttatcg ttaaagggat taatgctatc ttgggtattt ataattttat tgtcagaaaa
     9301 agcacgcaaa acataaagaa tagcatcaac ctctctaata ttagctaaaa actgattacc
     9361 caaaccctct cccctgcttg cgccctttac caagccagca atatcaataa aatcaacagt
     9421 ggcatatatt ttcttttttg attttgtcaa ctcagcaagt ttatcaaccc tcacatctgg
     9481 cacagcaaca acacccacat ttggatcaat agtgcaaaaa ggataattgg ccctatccac
     9541 ctgtttttta gttagtgttt gaaataaagt tgacttgcct acattgggca aaccaacaat
     9601 tccaatagaa agagacatag caatttaatt tttaattttt aatttttaat ttttaatttt
     9661 tatttgttat ttgtcattgc gaggcgaagc cgaagcaatc ccgtggaacg tttgtaaacg
     9721 cacaacgaga tcgccacgtc gtccactcac cttttactaa gatgagggac tcctcgcgat
     9781 gacaagaagg tatgttgcgt ttaattcttg aacctaattt acattgattt ttgatttttg
     9841 atttttgtga aacatttatg ccgaatgttt ttgtttcgga aaacaatcct caaaattata
     9901 aaatctttcc aaaaaacgta acaaaataac ttcctttacg accaacatat cttccaaaag
     9961 gaacccacaa gacattataa tcgcaaatta aagtgtattc ttttgtccta tccggttgaa
    10021 ttaaccaacc catcgttccg tctttatatg ctttctgcta aaatacttta tcataaatgg
    10081 ctttattatt cattcaagga gcccaagcaa acaaaaatca aagataaaga aaaacccgct
    10141 tctggcgttc cgcattgcgg aacaaagaca aaaaattcaa ttttgcgcct aaaatcgact
    10201 ggcgagccag caacatcttt ttatgattgg cggtggggac aggactcgaa cctgcaaacc
    10261 tctttcgagg ttcacgcttt ccaggcgtgt gccttgccaa ttcggcgcac cccaccagta
    10321 aataaatttt actctaaatt tcaattttaa gcaaacatca acaattattt aatgatttgg
    10381 aaagtaacaa cattattact tatctaggcc tctaattatt acctctaatt attattagaa
    10441 aaagttaacg ataaaaatag aagcggatcc ctatgtctat tgggatataa atcggatcca
    10501 attataacct ttattcattt aaattataaa aatatcttga gcatgatata aaaatgctca
    10561 tttttatttt gcttttaaac ataaatttga tataataaaa attaggaaag atttgtgaaa
    10621 ttaactcaaa aatttaaaaa ctatatataa tttatttcct tctttgtatt gataaattac
    10681 aaacaaaaag aagagtaaaa agaatacgaa gtattttgga agtaaaataa attttatgtt
    10741 taaacttcaa cgttattcta aaaacccaat cttaacacca ataaaagaac atccctggga
    10801 atcaaagatg gtttttaatc cagctgcagt tttaaaagat aataaggtgc accttattta
    10861 tcgtgcaatg ggtgaagacg aaattgatgg gtatcctatt tcacgattag gagcgtgtgt
    10921 gttaaaaaat gatggcgtaa caatagaaaa aagatacgaa aaaccaattt tagaaccaaa
    10981 aaaatggtat gagccagcag gatgcgaaga tcctagaatt actgaaatcg atggtaaata
    11041 ttatctttta tatactgctt atcttggcta taatgctccc ccgttattta aagaagagaa
    11101 aaccaatatt gctatggctg taagcgatga tctttttcat tggaaaagat ttgatcatct
    11161 tttattacca gatgtttttg aaccagaaaa gaacggcgtt ttgttcccca aaaaaattaa
    11221 tggatattac gtgctttttt atcgcattga accatcaata tatgtggctt atacaaaaaa
    11281 tttaacacct ttcccaccta aatggattgg ccacaaagta atagcaactc ccagggaaaa
    11341 ttcttgggac tcttggaaaa ttggagcagg cgctcctcct attgaaacaa aagatggttg
    11401 gcttttaatc tatcatggta tagacagacg aggagtttct cgaagagaaa aaaagaaaaa
    11461 agatgggaaa aaagaagaac gtatttatag attaggcgta atgcttcttg acaaagaaga
    11521 tcctgaaaaa attctctatc gttcaaagaa ttggatttta gaaccggaag agccttatga
    11581 gaaagaagga gctgtcccta atgtagtttt tacttgcggt gctgtagtaa taaaagacac
    11641 tctttttgtt tattatggcg gagccgatac tgttattgga gttgctacct gcagtttaaa
    11701 agaattgctt tctgctatta agaaaggaga agtttaataa ttttttaaaa aatgttaagc
    11761 gctaaaaaat acaaagggaa tccaatcatc gtcccctctt tagaaaaaaa agagtcctcg
    11821 atttataacc ccacaactgt tgttcgaaaa gaaaaggttt ttttgctggg ccgaagtgag
    11881 tttggatacg aaaataaaac catatctaaa attaatcttt tttcaagcca gaatggcttt
    11941 tcttttaaac cattccccaa aaacccagta ataaaaccag aaaaaaaata cgaaaaaatg
    12001 ggatgcgaag acccaagagt aataaaaaca gataagaata actattttct tacttacact
    12061 gcctacaaag gaaaagataa aaatggagat tataaaataa aattaatggg ggccctttca
    12121 aaagatttga tttcctggca aaaattcccg ctagttgaca atgaaaaatc cggagcggtt
    12181 gttcaaaatt acaaacacaa agaccaatat gtaatgtatt ttggaggtaa agaaatcaaa
    12241 attgcctttt ctcgagattt aaaaaaatgg aaatttaacg ccaaaccaat ttttactgca
    12301 agaaaaggaa atttttttga taattacttg gttgaacctg gcccgcctcc cattttaatc
    12361 aaaaaaggga ttcttttaat ttataatggt aaaaatgctg ctggaaaatt ctcagcaggt
    12421 tgggctattt ttgataaaaa caaccctgcg aaattattgg agcgttctcg aaaaccaatc
    12481 cttgaaccaa ctgaatattg ggaaaaatat ggaaaagtaa ataatgtaat ttttgctact
    12541 ggccttgtat tttttaaagg aaaatggctt ttatattatg gtggagctga caaaagtacg
    12601 ggcgtagcag tacttaattt ctaattttta atcatttata attttttaat ttaaaaacat
    12661 atgaataaaa attctttagg aatatgtatt gtatcaagct attcgccaag aaaatgcggc
    12721 atcgctactt atacttctag tttgaaacaa aatatcttaa aaattgctcc tgacacaaat
    12781 atcaaaattg tggctatcaa tgatcctcaa aaaaaatatc attattctaa aagagtgata
    12841 gcaacaattt cacaagaaaa aaaagaaaca tacataaaag cagcaaatgc aataaataaa
    12901 aacaaggaaa tagatgtagt aaatcttcaa catgtcttta gtttgtttgg aggaaaaaat
    12961 ggagaattta ttattgattt tcttaaacat ttgaaaaaac ctgtagttac tacaatgcat
    13021 atgatttatt ctgcgctgga aaaaccaaat tctcttgagg ttgtagataa aagttataaa
    13081 actataacag aaaaaatcat atccctaagc agcaaaataa tagtaataat tcaaccgatg
    13141 gcagatattt tagaatcgca ataccattgt tcttctgaaa aaattgccgt aatccctcat
    13201 ggggtaccag aggttaaaaa agaaaatcct aaaaaatata aaaaattaat cggctttgaa
    13261 aacaaaaaaa ttatttctac ttttggatta ataagaccaa agaagggact cgaatattta
    13321 atttgggcga tgccagaaat tgtaaagaaa tatccagaag ctatcgcttt gatcttaggt
    13381 gagtcacatc ctaataggcc aaaagaatat tataattttt taaaagaaga ggcaaaaaaa
    13441 accggtcttt tagataaaaa tatatatttc aatagccact atttaaaatt taaagaaatt
    13501 attacttatc tcttggcaac tgatgttttt attactcctt atcttgtacc agaacaaaca
    13561 tcaagtgggg taatcgctta tgctcttggc tgtggcaagg tggttgtttc tacgcctttt
    13621 ttgtacgcca aggaaatgtt aaaagacgga agaggaatat ttattaattt taaagactct
    13681 caaagcattt tttctgctat agattttata ttttcccatc ctgaggaaag aaaaaaaatt
    13741 gagagaaaag cctatatcac tgctcaaaaa atgctttgga gtaaagtatc aaaagaatac
    13801 ctcaaagttt ttaaagatgg tataaaaaac tatgaaaaaa aatgatctta aggttttata
    13861 tctttcttct ttcccgccac gacaatgtgg aatagccgat tttactcaaa acactattaa
    13921 tgcagtttat aaagtaaacc ctaatataga tcaaaaagta attgctatta atgaaaaaaa
    13981 tgcaccaaaa agagattact tagctacagt aaaatgggaa ctagaacaag aagatttaga
    14041 ttcttatcaa aaaactgcaa attatattaa tgagtgcgga gctgatgttt tatttttaca
    14101 gcatgaatat ggcctttttg gaggatttga tggcatattc gttttaaaat tattagaaaa
    14161 attgaaggtt cctattgttt ctttttacca tacaatccct cttataccta cctcaaaaag
    14221 aagagagttt cgtttaaata ttataaaaaa cattggtaat ataagtaagc atattattac
    14281 taccacccaa agtggcaaag aaatcttgga aaaagaatgt aaaattcctg taagaaaaat
    14341 ttctatcgtt catcatggcg gatatgatat tccctatccc aagcaaaaag acaaggaaga
    14401 attaaaaaag aaatacggtt ttcaaggaaa atttattgtt ttaacttacg ggcttattac
    14461 aaaatcaaaa ggaattgatt atgccataga agcaatagca aaattgaaag ataagcatcc
    14521 aaatatactt tatattattg caggcacttc tcatccagtc aaaattaaat tagcagaaaa
    14581 agattattat agcgatcttc aattaaaaac aaaaaaggca aaagccacaa acaatgttaa
    14641 gtttattaat aaatattttg aggaagagga acttatcgat tatctaaaaa tggctgatct
    14701 ttttttaatc ccttatctag taagagaaca aatttcaagc ggagtccttg ccaatgccgc
    14761 tactactgga aactgtattt taagcacacc atttccctat gccaaagaaa taatcggcga
    14821 agacgaagaa agaggatatt ttatagattt tgaaaacagt gattctattg ctcaaaaaat
    14881 tgctttttta atagaaaatc cgaaaattat tgagaaaaaa agaaaagaaa tgtatcaatt
    14941 cggtagaaat tttacttggc aaaaaatggc agaagagttt attaaaattc ttcaaaaaaa
    15001 atgatttttg aaaggtaact tttgtcttta aaaataactt taaaaaattt aaataatcaa
    15061 aaacggccct tttaagggcc gttttataaa aatatcctac tttttaatat ccttcaggca
    15121 taggtgaagg catctgatct ttcttttcct ctggtttttc agcaaccaca cactcagttg
    15181 ttaaaagcgt acctgctgct gaagcagcgt tttgaagagc acatctcacc acctttgttg
    15241 gatcaataat tcccgcagct agcaaatctt caaactgatt ggttttagca ttaaatccaa
    15301 aaccatcttt accatctttt actttttgaa ccactacccc atcatcaaca ccagcgtttt
    15361 tagaaattct acggatagga gattcaagca ctctttttaa aatcgaaagt cctacttttt
    15421 gttcttttac tacttttacc tcatcgagta cgggaatgca acgcaccaaa gcaactcctc
    15481 ctcctggtac aattccctct tcaatcgcag ctctcgtagc agctacggca tcttcaatct
    15541 tttgctgttt ttgtttttgt tcaacctcag ttgctgctcc tacttttatc acagctactc
    15601 cacctgaaag cttagccaaa cgttcttgta atttttctct atcaaattct gattcagttg
    15661 tttccatttc ttttctaact tgctcaatcc tagcatcaat atcttgtttt ttcccttttc
    15721 cctcaataat agtagtgttt tcctttttgg aaacaactct tcttgctcta cctaattgat
    15781 taatttctgt attttctaat ttcaaaccta actcttcagt aataacctgg cctcccgtta
    15841 acacagcaat gtcttgaagc atttcttttt ttctgtctcc gaatccaggt gccttaacag
    15901 caagggtatt aaatgtacca cgtagtttgt ttattactaa agttgccagt gcttcgcctt
    15961 ctacttcttc agcaataatt acaagatcct tacaccctga ctgacttaat ttttcaagca
    16021 agggtaaaat ttcttgtaaa gaagaaattt ttttatcagt aatcaaaata taaggatcat
    16081 cataaaccgc ctccatacga tcaggattgg taaccatata aggagaaaca taaccctgat
    16141 caaattgcat tcctttaaca atttcttttt gcagtccaaa ggtttttgat tcttctacag
    16201 taactactcc atctttgccc acttcatcaa atacctcggc aattaatttt cccatttttt
    16261 cgtcttctgc tgaaatagta gctacttgag ccatctcttc aaatcttgaa acttttttgg
    16321 aaatagattt taattgttgc acaacttttt ctactccttt atcaatgcct ctttttattt
    16381 ccaaaggatt agcacctgct gttacgttct taaatccttc tctaattaat tcttgagcaa
    16441 gaagagtagc agttgtagtg ccatcaccag ctacatcatt tgtttttgaa gcgacttctt
    16501 ttacaatctc tgcacccaag ttttcaaact tatcttctag ctcaatttct ttggcaatac
    16561 tcactccatc attggtaatt tctggtgctc caaatccctt atccagaaca acatttctgc
    16621 cctttggacc cattgtaatg gtcacagcat tcgctaattt atctacacct tttaaaagtt
    16681 tttctctagc tttttctgaa tactttatta tttttgccat attttttaag ctttttagct
    16741 ttttagcttt ttaaactttt ttaaaaagtt gcaagctaag aagctattct aaaattgcta
    16801 atatatcttc ttcttttgca ataagatatt ctttgtcatc gacctttatt tcgtcaggac
    16861 catatttttt aaagagcact ttgtctcctt ttttcacatc tggtgtcaat attttaccag
    16921 atgataattt tttgcctggt cccacggcaa taactgttcc ttcttgcggt tcttctttct
    16981 cagcggtttc gggtaataaa atcccgcttt ttgtcttttc ctcttttttt attggctcaa
    17041 tcaaaatata atctgcaagt ggtttaatgt tcataaaaca tctctcgaat aaggctcgaa
    17101 ttaatcgaat gtttctcgaa tgttcgagat ttattcggtt tcattcgtgg aaaattcgag
    17161 aattatttat tagcaaactc gacctttgat tgctaatcca ttttagcaaa acaaataccc
    17221 tctgtcaaga ggggtttgat aatatattta aggtaaattt tttaattaaa ataatttact
    17281 taacagcaca gctagatgta aacgaatcat taaaaatatc tattctatac agcaatcaat
    17341 tgctttttta accagttctc ctaattttcc caaaaatccc tttttttctt tatcgtgttt
    17401 atttaacgcc ttatcatcaa ttttctttac atcaccaaca tcaatcaatt ttaaattcaa
    17461 tccccaaaaa agagaatata aatcatatgc cttatcaaaa agattatatg cctcatcttt
    17521 tttccctttt cccaactgtt ttgtgccaac ctccattaaa cgcattgaag atgcaagcaa
    17581 atgcttagaa atacaccaca gttctccctc tggttcttta actatttttt tcaaaagttc
    17641 ctttcgcatc tcacgaacat cttttatcat attataatat tcaggctttt gaagtttggc
    17701 tccagtgaaa aagaaatgct cctcaatgct caccaaattc attattgcaa tactcaaatc
    17761 ctcatcagac gataaatcaa acttatcttt ttttttcatc tcatccactt ttttaataaa
    17821 ttcatcaaca ttttttatcg gatttttatc attatctttc atatatttat tttgttaaaa
    17881 aataataaag aacacttaaa attattaaag gcaaaataga tattacaact ttttgaaaag
    17941 gaaaataaac atggccgtta tttttttctt tcaagtaata atgccaattt gcaccacacc
    18001 aaaaagctat actccctaca ataattccaa aaagcaactt gtctagatga cagaaacata
    18061 aagtattcaa aggattgcct ataattcctg agtaaaaaag aggaattatt actaacgcaa
    18121 aataagcaat aactactaaa acagtccttc cccaaaaatg aatatttttc ttcgcaagcc
    18181 atccatctgt ccaaataatt aaagaaacaa ttaatccacc aatccataat cctgttatgg
    18241 tatcatcaat ccctagccag cgagaaagac caactcctgc gcccacagca atagtacaca
    18301 atggacaaac agccaatgct ttattgccaa aaagaaatcc agccactaaa aaagaaaaaa
    18361 gaaaaataag tgtctttttc atatttattg cccctcagcc attttttgtt caaaaaattt
    18421 gataatttcc tgttcgccca tcaaacagtt ttttccatcc caaagaaatg gtatcccaat
    18481 agaatcgcct gacaaaccac aatcttccgc tcgcatctga agttctttta aatttaaatt
    18541 attataataa acctcttttc gagtaaaaga aactttttct tgaacattat tttcttcaat
    18601 atattcttct ataattttac aatgaggaca atcatcgcta taaaaaagaa caacattatt
    18661 cattgactgt tgcattgctt cttgattttg ctgcgaggtc tcattgtttt ctattttatt
    18721 tgcattttct tcagacagaa caaagattga aaaaataaaa attgcaataa aaacaatagt
    18781 agaaaaaaca atttttttgt ccatatataa ataatattaa atctctttta atctgtcaaa
    18841 taaatttatc catcttcctt tgtaacaata aattatactt gcgaaaactc tgacatcatt
    18901 aataattgag tttttttaaa aagacaggat aagacaaata agattaaaat tttttatctt
    18961 atagcaaatt taaagtaaaa aaagctcaag gtctgtatag ggcaacgatc tactttcgca
    19021 gcaaaagcta ctatcatcga ccctgaagga tttcacttct gtgttcgaaa tgggaacagg
    19081 tggagcacct tcggtatcgt cgccttatac aaacctcaag ctttaattga tatggaatat
    19141 gtttttaaat ttgattccga aaacagtttc attctctata atcattcaat aaacttaaaa
    19201 cttaaaa
//
LOCUS       DAOTQD010000007        17513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_3245_length_17513_cov_8.249169, whole genome shotgun sequence.
ACCESSION   DAOTQD010000007 DAOTQD010000000
VERSION     DAOTQD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 17513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17513
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3245_length_17513_cov_8.249169"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1257)
                     /locus_tag="P5570_00615"
     CDS             complement(97..1257)
                     /locus_tag="P5570_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HRR39250.1"
                     /translation="MAKNTITSKKTLQRSYKNKQQKNIKIAIAGIGNCSSALVQGLFY
                     YKNINSNDNLVPGLMHNVLGNYKISDIKPVAAFDIDKRKVGKDLSEAIFALPNCTKTF
                     CKNIPKIGVKVKMGPILDGVADHMKNYPEEKTFVVAKEKPVDVVKELKNSGAEILINY
                     MPVGSQKATEFYAKAALDAGCAFINCLPVFIASDKNWAEKFEKKGLPTIGDDIKSQVG
                     ATITHRALTHLFTQRGVAIDKSYQLNFGGNTDFLNMLSRERLKSKKISKTESVESQLE
                     KRLSYDNLHIGPSDWVPFLKDNKVCYIRIEGRKFGDVPVELELRLSVEDSPNSAGCVI
                     DAIRLAKLALDRKIGGPLISSSAYFMKHPIQQFTDEKAREMVEEFIKGQRER"
     gene            1483..1785
                     /locus_tag="P5570_00620"
     CDS             1483..1785
                     /locus_tag="P5570_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRR39251.1"
                     /translation="MLPFLRLKQSNTIDNLWVYILSLIRKEPMHAYQIQKEIEKKFGF
                     QPGKITPYRVLYRLENDGFVKSSIKDRKRIYQITEKGEKELKKAKKFYQKILDELY"
     gene            1788..2822
                     /locus_tag="P5570_00625"
     CDS             1788..2822
                     /locus_tag="P5570_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRR39252.1"
                     /translation="MKKRELLILLSFLIGGAIIYFVFQKIGVKEIWEAFLSFSPWGVF
                     WVLILTVLYHLTAAWRLKVILEDKKYPISTKGVFSTWLASFALAYFTPIALMADGVLR
                     TYILKEKYSVPWKENIISNFIDKLLDGTIFFLTIITGIIFFILHNLTIPIKLWMAILI
                     LLFPIGGITFFYYRAFKNQSMLKLIEKPIQKMFKKLPERAFFWESELFDFFKSNNKNM
                     WKAMVFNFVRGIINWSRCWVLLLFLGLPINWIESLIIVGFTNLAYVLPIPAAIGSHEA
                     IQAFTFSKLGFQSHNAIAFTLILRAFDVLIGIVGIGIAFRFGTKRLRKIINNPSVEQN
                     EIENSSTSHH"
     gene            2893..4656
                     /gene="polX"
                     /locus_tag="P5570_00630"
     CDS             2893..4656
                     /gene="polX"
                     /locus_tag="P5570_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HRR39253.1"
                     /translation="MANQELAKILSEIALYLEMEDVPFKPQAYEKVAMILENLSEDVE
                     EIYKRGGEKAIENIPSVGKSIADKIIEYIQTGKIQYYEDYKKRMPVKIEELTAVEGIG
                     PKKVKALWQNLKIKDLKDLEKAAREHKIQTLFGFDKKSEKNILQAIEFLKKSKGRFLL
                     GEILPKAKEIEKELKGLKEVEEVSVAGSVRRRKETIGDLDFLVTLKKPNKETANKIMD
                     FFVSLPGVIKIWSKGTTRSSIRLKNANVTVDCDLRVVNKKSYGSALQYFTGSKEHNIA
                     TRVLAIKKGLKLNEYGVFKGKNMVAGWSESGVYRSIGLPWIEPELRENRGEIEAALRQ
                     QEGKKPGLPKIISYNDIKGDLHCHSNWNGGEDSIEDLAREAMKMGYQYLGISDHTKFL
                     RIERGLDEKQLEKQRKEIDKINSKFKIQNLKFKILQGCEANILPDGSIDIQDEFLKKL
                     DYVIAGVHSAFKMPKEKMTKRIIRAMKNPNVDIISHPTGRILKKRDEYQIDFDEILRV
                     AKETNTILEINSYPERLDLNDINIKKAKEAGVKMVINTDTHQKDQLRYIEFGIAMARR
                     GWAEKNDIINTYSLEEILNNF"
     gene            4709..6013
                     /gene="murA"
                     /locus_tag="P5570_00635"
     CDS             4709..6013
                     /gene="murA"
                     /locus_tag="P5570_00635"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRR39254.1"
                     /translation="MPTEYFQINGQKPLSGEIETKGCKNAATPIIAASLLTDEPCYIQ
                     NLPLVEDIFRMLEIIKDLGAKVEFQGEREIKIEAKNLDPSKIREDLVTMLRSSVFLLA
                     PLLVRFGKVEIPQPGGCLIGVRSIDAHLQGFQQMGVEIEKEGKHYLLSKKHLAPSKVI
                     LPEFSVTATENILMAASLLPGKTIIKIAALEPHVKDLSQVLVKMGARIHWLEDHTIEI
                     EGQKKLKGFSHRLIYDPVEAGSFIILAGVTKGDVIVKNVPLENLDLVLYKLKEFGINL
                     EKISGPQKYDDQSQIVFDVRVKSPQAFLPVSKVQVLPYPGVPTDLQCAFGVLATQSPG
                     ETLIHDPMYEGRLKYLEALNKMGADISVLDPHRAIVRGPSQLKGSSIENYDLRSGAAL
                     IITGLIAQGETLISNAYQVDRGYEKIEERLREIGADIKRVSF"
     gene            6032..6694
                     /locus_tag="P5570_00640"
     CDS             6032..6694
                     /locus_tag="P5570_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR39255.1"
                     /translation="MDIIWHGHSFFKINGKGEEGKVNIAIDPFDESIGLIPPQKVGAD
                     ILLISHYHSDHCNTKIIQGSPFIIDTPGEYEIKSIFIKGIPSFHDNVQGKERGLNTIF
                     VLDFKDEEIKMCYLGDFGETQPTPKTLEDIVGVDILFIPVGGKFTISGKEAAKLINQI
                     EPKIAIPMHYKIPGLNLDLDDEKSFLGAFGIGEKERIKKLKIKKDELKREGTEIVLLD
                     RC"
     gene            6753..9200
                     /gene="gyrA"
                     /locus_tag="P5570_00645"
     CDS             6753..9200
                     /gene="gyrA"
                     /locus_tag="P5570_00645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRR39256.1"
                     /translation="MAIQKRDISNEVKEAYLDYAMSVIVSRALPDVRDGLKPVHRRIL
                     YAMYEDGLRHNAKFRKCASVIGAVLARYHPHGDQAVYDALARMAQDFSLRYPLVQGQG
                     NFGSIDGDSPAAYRYTEARLSKIGEEMLKDIEKNTVDFIPNYDGTRKEPVVLPSPLPQ
                     LLLNGSFGIAVGMATNIPPHNLSELCDGLIYLLKKEDATTEEICEIIKGPDFPTGGQV
                     FDKKAIIKAYQDGRGAILNRGKAEIIEKTKDIFEIQIIEIPYGVQKSAMLQEMAGLVK
                     DKKIQGIKNILDESDKEGLRVTIELKKDANPQKILNQLFKSTSLERVFYLNMVALVEG
                     KQPATLSLKEALENFLLHRKEVTRRRTEFDIQKAKERIHILLGLKKALLHIDEIIAII
                     KKSKDRADAFKNLILRFKFSEIQANVILETKLQALARLEQQKILDELEEKKRLVEELE
                     GILKTKGGIEKVIEKELIEIKEKYGDNRKTEVIPQKVGELREEDLIPEEENILILTED
                     GYIKRISPRVFKAQKRGGKGVLGLALREEDKVSHFLYLSSHNQVCFFTNLGKVFKIPA
                     YNIPETDRQGLGRGILNFLELSSQERITSIVAMPHLKESDKAKYLVMVTKNGVIKKTK
                     ISDFEQVRRSGLRAIKLEDGDQLVFAKVIEEGSEILLASSAGNIIRFKEEELRPMGRN
                     ATGVRGMRLNLKSQTSNLKTDDKIVGMEIIKNQNYLLTLSENGFGKRTDIQKFRLQKR
                     GGKGIMGMKITEKTGSLARIGLLTKDEEEDLIVISKKGQTIKTKIKQVPILGRVSQGV
                     RVIKLKKGDKVASAITI"
     gene            9294..9842
                     /locus_tag="P5570_00650"
     CDS             9294..9842
                     /locus_tag="P5570_00650"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR39257.1"
                     /translation="MTTFQPGTGGFLNPQNIVDNLEIKEGMIIADFGCGNGYFTLPLA
                     KKVSEYGKVFALDILPDALETLSSRAKTEGINNIQLKRCNLEKENGSGLLDNSCDMVF
                     LTNLLFQTENDENVVKEAYRILKPGGELIFIDWRPDVALGPKGKRVNPEEIKELMSKE
                     KFSFEKEIPTDKYHFGMIFKKL"
     gene            9839..10180
                     /locus_tag="P5570_00655"
     CDS             9839..10180
                     /locus_tag="P5570_00655"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39258.1"
                     /translation="MKKVIKSIIIVLGFLFLIGNLTFAVSFAVSIKNPLKYDTIPEII
                     KAICDFIFWIGLAIVPLMIIIGGIMFLTAGGEPSKVSKAKNLLLWSAVGLAVLLSARA
                     ISSIIETVIGI"
     gene            complement(10268..11161)
                     /locus_tag="P5570_00660"
     CDS             complement(10268..11161)
                     /locus_tag="P5570_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39259.1"
                     /translation="MIDDEIDWISEEADQAVETEEQTEEQTEEQTEEQTEEQEEKCVE
                     KKERSFAVVILIAAIAAIAAIGGGYMMLHPSTQEKSTPVTELTAVPTTPTPTIHIPIP
                     GIEGVNLLIRGNEVAFERSGTSVERTDFYKMDSNIVAFTKNKDKAVLYWGEPVIKIYG
                     ISEIYDVDQGIYKEVLNTTPTPSTESRGNAWEVNQKLFTGQEVKISGVLIDFNKREIR
                     AMIATDDIWWSEENPQTIKKNIFTGITPDGQTLFQVDFEKETVWVYKLINIAPITVPS
                     APVEISPIQEMTVFPAKIPSN"
     gene            11383..11976
                     /locus_tag="P5570_00665"
     CDS             11383..11976
                     /locus_tag="P5570_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39260.1"
                     /translation="MRNKKETFILIGGCFAFLLLCGGFVWAAIHGPNECCKLNHDLTD
                     IDSVCSEGAIIAAKGCDEFTCPGGNVPTCDRNTNACNCTGAGSCDDSGFKCWCDINDD
                     EINDAPCAISTSNTTKSWGTCCVIDTVYNVTDWLFYILVAAIAILFIIAGILFLTAGG
                     DPAKISKSKTMILYGLVGLALALLARIVPAIIKSIVS"
     gene            12047..13204
                     /locus_tag="P5570_00670"
     CDS             12047..13204
                     /locus_tag="P5570_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRR39261.1"
                     /translation="MEIKKIIFIFSLFFLIMGMGNKVFATACYDRDDAERIPDIYNKG
                     KCYDVQKEKWFEESCKDENTLIEYYCTNSGCQEEEKICPSGYSCSDGACRKIESCSDS
                     DPYKDIYQRGSCEEYSSSGTERFIDTCASDNKTLIEYYCTPGGKKCQSFTFDCSTIGG
                     GTCNNGKCTKYPSYEGEQKCYDSDGGVKPNIAGWCRDVEGQTNMQDSCNNGVLKEYYC
                     EKDLERCIFKTINCSQGCDGPYKCVGTGAATALEPSKPTGANECCRLSHNLTDVDSVC
                     SKDKVVGPTNPQWCDVNGDGTQDSVDNRTVKWGTCCLLDSIYTVSDWIFIMSFPLSIL
                     IFLVAGYFLLASGGDPQKVKNGKNLLTWGAIGLTIVILSKIIPAIIKAIVA"
     gene            13209..13679
                     /locus_tag="P5570_00675"
     CDS             13209..13679
                     /locus_tag="P5570_00675"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39262.1"
                     /translation="MKKSAVAYIFLMSLLVLSMPFLAKGDIHGPNECCFLEHDLTDVE
                     NGCDRGNIVGAQNPKWCDVDGDGIRDYIDGSPSKWGTCCLLDSVYTVSDWLFIIFIAL
                     TLIIFGIAGYIFLFSGGDPQKNSKAQKFLIYGAVAIALVALSKIIPAIIKAIVA"
     gene            13815..14267
                     /locus_tag="P5570_00680"
     CDS             13815..14267
                     /locus_tag="P5570_00680"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRR39263.1"
                     /translation="MMKKFFAVILTLVMVSLIAAPMIASAQTGAKECCQVKRNYTDLD
                     GNCGKGKIVGPAGGSCDIGDINCSSNDWGTICLMETVYNVTDWLFYIVLSIAVIFGVI
                     SGFMFMISSGDPTKTEKARNLLMYMAIGLVVAALAKVIPSIVRTMVGV"
     gene            complement(14332..14413)
                     /locus_tag="P5570_00685"
     tRNA            complement(14332..14413)
                     /locus_tag="P5570_00685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14377..14379),aa:Leu,seq:tag)
     gene            complement(14437..16146)
                     /locus_tag="P5570_00690"
     CDS             complement(14437..16146)
                     /locus_tag="P5570_00690"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRR39264.1"
                     /translation="MKKGLLIFCITAIVVIAVAFYILYLPKDSFARGITVGGEKIGGL
                     SKTQAENLIETKVNEFLEKDITFHFSNSEGIYSKEIKIKDLGIVFDIEKSLEEPFSVG
                     RKFSSNSSINDFFQNTKEKIASLKGNYQFSLIATIDGKEEKEEGFEKFFTENFGKFEI
                     PPQNAVVIFNEKTLDFDIEEPKEGLLFSREKIKKEIEKNAGFLIISNIYLVLEDASPE
                     ITQEMALSAANQAKEILKAGPYVLLAEEKRIEVPQKTLGNWFSFIPQKKDNQITLSPS
                     LNEELIQNYLAEISNSINIEARNPVLSFEEGVLKIIIAPKTGKILKIEESAKKIQENI
                     LAKEKQISLRFEEVEPKITEEKINELHIEKLIGTGTSNFAGSPKNRIHNIKIATAKFN
                     GMLIAPDEEFSFNQALGEVGPAQGYLPELVIKNNKTVPEYGGGVCQVSTTMFRAAVYS
                     GLEITERKSHAYPVKYYNPQGFDATVYLPSPDLKFKNNTGNWILIQAKIKDNNLTFEF
                     YGKDDGRKVIVKGPYQYDFQEDGSMKARLEEEVWKNGELILKKTFLSFYKSPNLYPTT
                     NAE"
     gene            complement(16172..16609)
                     /locus_tag="P5570_00695"
     CDS             complement(16172..16609)
                     /locus_tag="P5570_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR39265.1"
                     /translation="MSDFFEKLSNNEGGINNENLGVQNGPSTRETENSEEGQLAVDVF
                     QTDSDLVIQSTIAGVKSEDLDISIQNDMVTIRGERKKELEVNPQDYFHQECYWGAFSR
                     QIILPEEIDPSKAKAELKNGILTLRLPKLEKTKTTKIRVTEVK"
     gene            complement(16687..17403)
                     /locus_tag="P5570_00700"
     CDS             complement(16687..17403)
                     /locus_tag="P5570_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HRR39266.1"
                     /translation="MSISTFILYFVIGALPAIVWLLFYLRQDVHPESNKKIIEIFLWG
                     GIMVVPVYLAETILECFFPEKDILLQNTILLFIYYIFVVGVVEEFFKYWVVKKRVINS
                     SHLDEPIDLMLYLIISGLGFAMVENLSVILSSAALEEAIFVSLIRLLTAVFLHSLTAA
                     ITGYFLAVSLYNRKKIFIAVGILIAATFHGLYNLSIIELNSAENLFSFLLPIVIIFLM
                     AIIVYIFFKKVKCLPRGSKI"
BASE COUNT         5742 a         2556 c         3316 g         5899 t
ORIGIN      
        1 tgtcatcgcg agcgaggtca aagaccgagc gtggcgatcc cgtggcattg ttgcgttaaa
       61 tttttgaatc tacattagta aatttattga caattcttat ctttctcttt gacccttaat
      121 aaactcttca accatttctc gtgctttttc atcggtaaat tgttgaatag gatgcttcat
      181 aaaatatgct gaagaagaaa ttaaggggcc gcctattttt ctatcgagag ctaattttgc
      241 taatcttatt gcgtcaatca cgcagccggc agaatttggt gagtcctcaa ctgaaagacg
      301 caattctagt tccactggca catcgccaaa ttttcgtcct tcaatcctaa tataacaaac
      361 cttattatct tttagaaaag gaacccagtc agaaggacca atatgcaaat tatcataact
      421 caatcttttt tccaattgtg attcaacgct ttcggttttt gatatttttt ttgattttaa
      481 cctttctcgt gaaagcatat ttaaaaaatc tgtatttcca ccaaagttca actgataaga
      541 tttatctatt gctactcctc tttgagtgaa gagatgggtg agggcgcgat gggtgatagt
      601 ggcgccaacc tgagatttta tatcatcacc aattgtgggt agtccttttt tttcgaattt
      661 ttctgcccag tttttgtcag aagcaataaa aactggcaaa caattaataa aggcgcaacc
      721 cgcatcaaga gcagcctttg cgtaaaattc agtagctttt tgagatccca ctggcatata
      781 gtttattaaa atttctgctc ctgaattttt taattctttt accacatcaa ctggtttttc
      841 tttagccaca acgaaggttt tttcttcggg ataatttttc atatgatcag ccacaccgtc
      901 taggattggt cccattttta ccttgacgcc gatttttggt atatttttgc aaaaagtttt
      961 tgtgcaattg ggaagagcga aaatagcttc tgaaagatct ttcccaactt ttcttttatc
     1021 aatatcaaaa gccgctactg gtttaatatc agaaatttta taatttccaa gaacattatg
     1081 cattaatcca ggaaccaaat tatcattact gtttatattt ttataataaa aaagaccctg
     1141 aacaagagca gaagaacaat ttccaattcc agctatcgca attttgatat ttttttgctg
     1201 cttattttta taagaccgtt gcaaggtctt ttttgatgtg attgtatttt ttgccataaa
     1261 tttattttat ttattaaaat aatttctaat tttttgacct ttcctacttt tatactattt
     1321 tactcatgta aaatactttg tcaagaggca atcttgtttt ttcgtaatta gctttaaatt
     1381 aatgattggt tgtcctcctc ttttttttag cattgttttt tgatagaatt gaaagaagct
     1441 atcaagctcg taatttatta attagttaat ttggtatttt ttatgttgcc tttcttgcgt
     1501 ctcaaacaat caaacacaat tgataattta tgggtttata tcctttcgtt gattagaaaa
     1561 gagccaatgc atgcttatca aattcaaaaa gagattgaaa aaaaatttgg ttttcagccg
     1621 ggcaagatta cgccttatcg ggtattgtat cgtctagaaa acgatggttt tgttaaaagt
     1681 agcataaaag acagaaaaag aatttatcaa attactgaaa aaggagaaaa agaacttaaa
     1741 aaagcaaaaa aattttatca aaaaatttta gacgaattat attaaaaatg aaaaaaagag
     1801 aattattaat tttattaagc tttttgattg gtggcgctat tatctatttt gtttttcaaa
     1861 agattggagt aaaagaaatt tgggaggcgt ttttgtcttt ttctccatgg ggtgtttttt
     1921 gggttttaat tttaactgtg ctttatcatc taacggctgc ttggcgcctt aaggtaattt
     1981 tagaagataa aaaatatcct atttctacta aaggagtttt ttctacttgg cttgccagtt
     2041 ttgctctcgc ttattttact ccaatagcac taatggctga tggagttctg aggacgtaca
     2101 ttttaaagga aaaatattca gttccctgga aagaaaatat tatttccaat tttattgata
     2161 aattattaga tggaacaatc ttttttttaa ccattattac gggaataata ttttttatct
     2221 tacacaacct taccattcct ataaaattat ggatggcgat tttgatttta ctttttccaa
     2281 ttggtggcat tacttttttc tattatagag cttttaagaa tcagagcatg ttaaagttga
     2341 ttgaaaagcc aattcaaaaa atgtttaaaa aattaccaga aagggcattt ttttgggaaa
     2401 gcgaattatt tgattttttt aaatcaaata ataaaaatat gtggaaagca atggttttta
     2461 attttgtaag aggaattatt aattggagta ggtgctgggt actgctttta tttttaggtc
     2521 ttcctattaa ttggatagaa tctttaatta ttgttggttt tactaattta gcttatgttt
     2581 tgcctattcc agccgctatt ggaagccatg aagctattca agcatttact ttttcaaaat
     2641 taggatttca atctcacaat gctatcgcct ttactttaat tttaagagca ttcgatgttt
     2701 tgatcggaat tgttggaatt ggaattgctt ttaggtttgg aactaaacgt ttaagaaaga
     2761 ttataaacaa tccgagcgtg gagcaaaacg agatagaaaa ttcatcaaca tcgcatcatt
     2821 aatagaacga gatcatttat cgttctatta ctcaaggata attcgattta atgtgcggat
     2881 tattcatata atatggcaaa tcaagaatta gccaaaattt tatcagaaat tgctctctat
     2941 cttgagatgg aagatgttcc ttttaagccc caggcctatg aaaaagtggc aatgatttta
     3001 gagaatctaa gcgaagacgt ggaggaaatt tataagagag gaggagagaa ggcaattgag
     3061 aatattccga gcgttggaaa gagcattgct gataaaatta ttgaatatat tcaaactggt
     3121 aagattcaat attatgagga ttataaaaag agaatgccag taaaaattga agagctaact
     3181 gcggttgagg gaattggtcc taaaaaagtt aaggcgttgt ggcaaaattt aaaaattaaa
     3241 gacctaaaag atttagaaaa agcagcaaga gaacataaga tccaaactct ttttgggttt
     3301 gataaaaaaa gcgaaaaaaa tattcttcaa gcaatagaat ttttaaaaaa atcaaaaggg
     3361 agatttttgt tgggtgaaat tttaccaaaa gctaaagaaa tagagaaaga attaaaagga
     3421 ttaaaagagg tggaggaagt ctctgtggct ggttcagtaa gacgtcgaaa ggaaaccatt
     3481 ggtgatcttg attttttagt aaccttaaaa aaaccaaata aagaaacagc aaataaaata
     3541 atggattttt ttgtttcctt gcctggtgtg ataaaaattt ggagcaaggg aacaactaga
     3601 tcttcgatta gattgaaaaa tgcgaatgtg acagttgatt gtgatttaag ggtggtaaac
     3661 aaaaaatctt atggttcggc tttacaatat tttactggtt caaaagaaca taatattgct
     3721 actagggtgt tggcaataaa aaagggatta aaattgaatg aatatggagt atttaaagga
     3781 aaaaatatgg tggcaggttg gtcagaaagt ggcgtttaca gaagtatcgg tctcccttgg
     3841 atagaaccag aactaaggga aaacagggga gagattgagg cagccttgcg tcagcaagaa
     3901 ggtaaaaaac caggattgcc caagattatt agttataatg acattaaagg agatttgcat
     3961 tgccattcaa attggaatgg tggagaagat tctatcgagg acctagcaag ggaagcaatg
     4021 aaaatgggtt atcagtatct ggggatttca gatcatacca aatttttgcg tatcgagcgt
     4081 ggattggatg aaaaacaatt ggaaaaacaa agaaaagaaa tagataaaat aaattcaaaa
     4141 ttcaaaattc aaaatctgaa attcaaaata ttgcaagggt gtgaggcaaa tattctaccc
     4201 gatggttcaa ttgatattca agatgaattt ttaaaaaaat tggattatgt gattgctggt
     4261 gttcattctg cttttaaaat gccaaaagag aaaatgacta aaagaataat tcgggcaatg
     4321 aaaaatccaa atgttgatat tatttctcat cccacgggtc gaattttaaa gaaacgggat
     4381 gagtatcaga ttgattttga tgaaatttta agagtagcca aggagacaaa tacaattttg
     4441 gaaattaatt cttatcctga gcgtcttgat ttaaacgaca ttaatataaa aaaagcaaaa
     4501 gaggcaggag taaaaatggt aatcaacact gatacccatc aaaaagatca attacgatat
     4561 atagaatttg gtattgccat ggctcgtcgc ggttgggcag aaaaaaatga tattattaac
     4621 acctattctc ttgaagagat attaaataat ttttaaaaac atcgttctta gttgacaaaa
     4681 tgtctgctat tggtaaaata ttattgatat gcctacagaa tattttcaaa ttaatggtca
     4741 aaaaccgctt tctggggaga ttgaaacaaa aggatgcaaa aatgctgcta ctcctattat
     4801 tgctgcttct cttttaacag atgagccttg ttatattcaa aaccttcctt tggtagagga
     4861 tatttttcga atgttagaaa ttataaagga tttaggtgcc aaggtagaat ttcagggaga
     4921 aagagaaata aaaatagagg caaaaaatct tgatccttca aaaattagag aggacctagt
     4981 aacaatgttg cgttcatcgg tatttctttt ggccccttta ttagttcgat ttggaaaagt
     5041 ggaaattcct caacccggcg gatgtttgat tggggtacgt tctattgatg ctcacttgca
     5101 agggtttcag cagatggggg ttgaaattga aaaagaaggg aagcattatc ttttatcaaa
     5161 aaagcactta gctccatcaa aagtaatatt gccagaattc agtgtaactg caactgaaaa
     5221 tattttaatg gcagcatctc ttttgcctgg caaaaccatt attaaaattg ctgctcttga
     5281 accccatgtg aaagatctta gtcaagtgtt ggtaaaaatg ggagcgcgaa ttcattggtt
     5341 ggaggatcat actattgaaa tagaaggaca gaagaaatta aaaggatttt cccatcgtct
     5401 tatttatgat ccagttgaag caggttcatt tattattttg gcaggcgtta ccaaaggaga
     5461 tgttattgta aaaaatgtac ccctggaaaa tttagattta gtactgtata aattaaaaga
     5521 atttggtatt aatcttgaaa aaattagcgg accccaaaag tacgatgatc aatctcagat
     5581 agtttttgat gtaagggtaa agagtccgca agcgttttta cctgtttcaa aagttcaggt
     5641 acttccttat cccggtgttc caactgatct tcagtgtgct tttggggttt tagccactca
     5701 gagtccaggc gaaactttaa ttcatgatcc gatgtatgaa ggacgattaa aatatcttga
     5761 agcccttaat aagatggggg cagatatttc agtattggat cctcatcggg ctattgtaag
     5821 agggccatct caattaaaag ggagttcgat tgaaaattat gatttgagat ctggcgctgc
     5881 tttgattatc accggtttaa ttgcccaggg agaaaccctg atttctaatg cttatcaagt
     5941 agaccgaggg tatgagaaga ttgaggaacg tcttcgagaa atcggcgcag atattaaacg
     6001 agtatcattt taattatatc aatttaaaat tatggatatt atttggcatg gtcattcttt
     6061 ttttaagatc aacggaaaag gagaagaggg aaaagtaaat attgctattg atccttttga
     6121 tgaaagtatt ggtcttattc ctcctcaaaa agtaggagct gatatcctat tgatttcaca
     6181 ttatcattct gatcattgta atacaaagat tattcaaggg tctcctttta ttattgatac
     6241 gccaggcgaa tatgaaataa aaagtatttt tataaaaggg atacctagtt ttcacgataa
     6301 tgttcaagga aaagaaagag gattgaatac aatttttgta ttagatttta aagacgaaga
     6361 aattaaaatg tgctacttgg gggactttgg agaaacccag cccacgccaa aaacattaga
     6421 agatattgtg ggagtggata ttctttttat cccggtgggt ggaaaattca ctatttctgg
     6481 aaaggaagca gcaaaactta ttaatcagat cgagccaaaa attgcaatac cgatgcatta
     6541 taaaattcct ggtttaaact tagatttaga tgatgaaaaa tcttttttgg gagcttttgg
     6601 aattggtgaa aaagaaagaa taaaaaaatt aaaaataaaa aaggatgaat taaaaagaga
     6661 ggggactgaa atagtattgc tagacagatg ctaggtgatt taataatatt aatttacgag
     6721 tttacaatta atagattaaa aatttattat ttatggctat tcaaaaaagg gatatttcaa
     6781 atgaggtgaa agaggcatat cttgattatg caatgagcgt aattgtttcg cgtgctttgc
     6841 ctgatgtgcg tgatggttta aaaccggttc atcggcgaat tttgtatgca atgtatgaag
     6901 atggtttaag gcacaatgca aagtttcgaa aatgcgcttc ggttattgga gcggtattgg
     6961 cccgatatca tcctcatggc gatcaggcag tttacgatgc cctagcaaga atggctcaag
     7021 atttttcttt aagatatcct ttggttcagg gacaagggaa ctttggaagc atagatgggg
     7081 attccccagc tgcatatcgt tataccgaag cccgtctttc aaaaattgga gaagagatgt
     7141 taaaagatat tgaaaagaat acagttgatt ttataccaaa ttatgatggc acaagaaaag
     7201 agcctgttgt tttgccatcg ccccttcctc aattactatt aaatggttct tttgggattg
     7261 ctgttggtat ggcaactaat attcctcctc ataatctttc agaactttgt gatggattaa
     7321 tttatctttt gaagaaagaa gatgccacaa cagaggaaat atgtgaaatt attaaaggtc
     7381 cagatttccc tactggtggt caggtttttg ataaaaaagc cattattaag gcctatcaag
     7441 atggacgagg agcaattttg aatcggggaa aggctgaaat tattgaaaaa acaaaagata
     7501 tatttgaaat tcaaattata gaaattcctt atggtgttca aaaatcagca atgcttcaag
     7561 aaatggccgg attggtgaaa gataaaaaaa ttcaaggaat aaaaaatatt cttgatgaat
     7621 cagataaaga aggactgcgt gttactattg agcttaaaaa agatgccaac cctcaaaaga
     7681 ttttaaatca actttttaaa tcaacttctt tagaaagggt tttttatctt aatatggtgg
     7741 cgctggtaga gggaaaacag cccgctaccc tttcattaaa agaagcgctg gaaaattttc
     7801 ttttgcacag aaaagaggtg acacggcgtc ggactgaatt tgacattcaa aaggcgaaag
     7861 aaagaattca tattttgttg ggtttaaaaa aagcattact gcacattgac gagattattg
     7921 ctattatcaa aaaatcaaag gacagagcag atgcttttaa aaatctaatt ttaagattta
     7981 agttttcaga gattcaagca aatgttattt tggaaacaaa actgcaagcg cttgctcgcc
     8041 ttgaacaaca aaagattcta gatgagcttg aggagaaaaa aagattagta gaggaattag
     8101 aaggtatttt aaaaacaaaa ggaggaattg agaaagttat tgaaaaagaa ttaatagaaa
     8161 taaaagagaa atatggggat aatcggaaaa ccgaagttat tcctcaaaaa gtgggtgaac
     8221 ttcgcgagga ggatttaatt ccagaagaag aaaacatttt gattttgact gaagatggtt
     8281 atataaaaag aatttctcca cgagtattta aagctcaaaa gagaggagga aagggcgtgt
     8341 taggactagc tcttagagaa gaagacaagg taagtcattt tctttatttg tcaagtcata
     8401 atcaggtttg cttttttact aatttaggca aggtttttaa gataccggct tataatattc
     8461 ctgaaacaga tcgacaggga ttaggcaggg gtattttaaa ctttcttgaa ctttcttctc
     8521 aagaaagaat tacctcgatt gttgctatgc cgcatttaaa ggaaagcgat aaagcaaagt
     8581 atttggtgat ggttacaaaa aatggtgtta ttaaaaaaac aaaaatcagt gattttgaac
     8641 aggtacgacg atcggggcta agggctatta aattagaaga cggagatcaa ttagtgtttg
     8701 ctaaggttat tgaagaggga tcggaaatat tattagcttc ttctgcaggc aatatcattc
     8761 gttttaaaga agaagaatta agaccgatgg gaagaaatgc tactggtgta agggggatga
     8821 ggttgaatct caaatctcaa acttcaaatc tcaaaaccga tgacaaaatt gtgggaatgg
     8881 aaattatcaa aaatcaaaac tatctactga ctttatctga aaatggattt ggaaaaagga
     8941 ctgatattca aaagtttcgt ttgcaaaaaa gaggaggaaa aggtataatg ggaatgaaga
     9001 taacagaaaa aactggtagc ttggcacgga ttggtctgtt aacaaaagat gaagaagaag
     9061 atttaattgt tatttcaaaa aaaggtcaaa caattaaaac aaagattaaa caggtgccta
     9121 ttttggggcg tgtatctcaa ggagtaagag tgattaaatt aaaaaaagga gataaggtgg
     9181 catcagcgat aacaatataa aaaataaaat ctcgaatatt ctgtggatta aatcgaataa
     9241 gttttgaata ttcgatggta ttcgcggcaa tttaaaaaaa attcgagaaa cttatgacaa
     9301 cttttcagcc gggcacagga ggatttttaa atcctcaaaa tattgttgat aacttggaaa
     9361 taaaagaagg gatgattatt gctgattttg gttgtggtaa tggttatttt actttgccct
     9421 tagcaaaaaa ggtaagcgaa tatggaaaag tatttgcctt agatatctta ccagatgcgc
     9481 ttgaaactct ttcttcacga gcaaaaacag agggaattaa taatatacaa ttaaaaagat
     9541 gtaatcttga aaaagaaaat ggttcaggac tgcttgataa ttcctgtgat atggtttttt
     9601 taacaaatct tttatttcaa accgagaacg atgagaatgt tgtaaaggaa gcatacagga
     9661 ttttaaagcc cggtggagaa ttaatattta ttgattggcg accagatgtt gctttgggtc
     9721 cgaaaggaaa acgggttaat ccagaggaaa taaaagagtt aatgtcaaaa gaaaaattct
     9781 cttttgaaaa agaaattcca actgataaat atcattttgg aatgatattt aaaaaactat
     9841 gaaaaaagta attaaatcta taataatagt tttaggattt ttatttttaa tagggaattt
     9901 aacctttgct gttagttttg ctgttagtat taaaaatccc ttaaaatacg atacaattcc
     9961 agagatcatc aaagccattt gtgattttat tttttggatt ggtttggcta ttgttccttt
    10021 aatgataatt attggaggaa taatgtttct tacagctggc ggagaaccat caaaagtttc
    10081 aaaggcaaaa aatttacttt tgtggagcgc tgttggcctt gcagttttac tttcagccag
    10141 ggctataagt tcaatcattg aaacagtaat tggaatataa gtatatttta aataaaaaaa
    10201 caccaccctt cttttaaatt aaaaattaaa agggtggttt ttaaaatatc tttgcctttg
    10261 tagtctctta gttagatggg atcttggctg ggaaaaccgt catttcctgg ataggggata
    10321 tttctacagg cgctgatggt actgtgatcg gtgcaatgtt aataagttta tacacccaaa
    10381 ctgtttcttt ttcaaaatcc acttggaaaa gagtttgacc atcgggagta atccccgtaa
    10441 aaatattttt ctttattgtt tggggatttt cttcactcca ccagatatcg tcagtagcta
    10501 tcattgccct gatctctctt ttattaaaat caatcaaaac gccagagatt ttcacttcct
    10561 gtccagtaaa taatttttga ttaacttccc aagcgtttcc tctcgattcc gttgagggag
    10621 taggagtagt atttaatact tctttataaa ttccctgatc cacatcataa atttctgaga
    10681 taccgtaaat tttaattact ggttctcccc aatagagtac tgccttatct ttgtttttag
    10741 taaatgctac tatattacta tccatcttat agaaatcagt tctttcaact gaagtgccgc
    10801 ttctttcaaa tgccacttcg tttcccctta ttaaaagatt tactccttca atgccaggga
    10861 taggaatgtg gattgtcggt gtcggtgtcg ttggaaccgc cgttaattcc gttactggcg
    10921 ttgacttttc ctgtgtcgat gggtggagca tcatatatcc gcccccaatt gcagcaattg
    10981 cagcaattgc tgcaattaaa attaccactg caaaactgcg ttcctttttc tccacgcatt
    11041 tctcttcttg ttcttctgtt tgttcttctg tttgttcttc tgtttgttct tctgtttgtt
    11101 cttctgtctc tactgcttga tctgcttctt cgctgatcca atcgatttca tcatctatca
    11161 tttttacctc ctaattttta aagatttttc ctctgaaaga attcagagag caagataaga
    11221 aaaattttct tacctcacgc ctaaagtaaa tcacatttct ttttacttgt caagatttac
    11281 cattttttta atttattaga ttgttcaaaa atattacaaa atagccaaga cgttttgttt
    11341 gacattttat tatttttttt ggtataattt aaaatagagg atatgagaaa taaaaaagag
    11401 acttttattt taatcggcgg gtgttttgca tttttattgc tttgcggtgg ttttgtatgg
    11461 gctgctattc atggtcctaa tgaatgttgt aaacttaacc atgatcttac tgacatagat
    11521 agtgtttgca gtgagggtgc aataatagca gcaaagggat gtgatgaatt tacctgtcct
    11581 ggtggcaatg ttcctacttg tgacaggaac acaaacgctt gtaattgtac tggtgctggt
    11641 agttgtgacg acagtggttt taaatgttgg tgcgatatta atgatgatga gattaatgat
    11701 gcaccctgtg caatctcaac aagcaatact actaaaagtt ggggcacttg ttgtgtaatt
    11761 gatacagttt acaatgtgac agattggtta ttttatattt tagtggcagc aattgctatt
    11821 ctttttatca tagcaggaat tttattttta acagctggcg gcgatcctgc aaaaatttca
    11881 aaatcaaaaa caatgatatt atatggattg gttggattgg ctttggcatt gttagcaaga
    11941 atagttccag caataattaa gagcatagta agttaaaatt ttaaaattga aacaagtttg
    12001 tattttcatt tacgtacgct ttgcccgctt gtggcgggtt cgccctatgg agattaagaa
    12061 aattattttt atttttagtt tgttcttttt gataatggga atgggaaata aggttttcgc
    12121 aacggcttgt tatgatagag acgatgcaga gagaatacct gatatataca ataaaggaaa
    12181 atgttatgat gtgcaaaagg aaaaatggtt tgaagaatct tgtaaagatg aaaacactct
    12241 aatagaatat tattgtacta atagtggttg tcaagaagaa gaaaaaatat gcccctccgg
    12301 ttatagttgt agtgatgggg catgcagaaa gatagagagc tgcagtgatt ctgatcctta
    12361 taaagatatt tatcaacgag gtagttgcga agaatattct tcgagcggaa ctgagagatt
    12421 tattgatact tgcgccagcg acaacaagac attaatagaa tattattgta ctcctggtgg
    12481 aaaaaagtgt cagtcattta cttttgattg ttcaacaata ggtggtggta cgtgtaataa
    12541 tggtaaatgt actaaatatc caagttatga aggagagcag aaatgttatg atagtgatgg
    12601 gggagtaaaa ccaaatatag cagggtggtg tagagatgtt gagggtcaaa caaatatgca
    12661 agatagttgc aataatgggg tcttgaaaga atattattgc gagaaagatc ttgaaagatg
    12721 tatttttaaa accattaact gttcccaagg atgcgacggt ccttataagt gcgtgggaac
    12781 gggagctgca actgctcttg agccctcaaa accgactggc gctaatgaat gttgtcgttt
    12841 aagtcataat ttaactgatg tggatagtgt ttgcagtaaa gacaaggtgg tgggacccac
    12901 caatcctcag tggtgtgatg taaatggcga tggtacacaa gacagtgttg ataatagaac
    12961 tgtaaagtgg ggaacttgct gtcttttgga ttctatttat actgtttctg actggatatt
    13021 tataatgtct tttcccttat caattcttat ttttttggtg gctggttatt ttcttttagc
    13081 ttctggcggt gatcctcaaa aagtcaaaaa tggaaaaaac cttttaacct ggggagcaat
    13141 aggattaaca atagttattt tatcaaaaat aattccagca attatcaagg caattgttgc
    13201 ctaaaaatat gaaaaaatct gctgttgctt atatattttt aatgtcgcta ttagttttat
    13261 caatgccttt ccttgcaaaa ggggatattc atggtccgaa tgaatgctgt tttttagagc
    13321 atgatttaac tgatgtagag aatgggtgtg acaggggaaa tattgttggg gcccaaaatc
    13381 caaaatggtg tgatgttgac ggagatggaa tacgagatta tattgatggg tctccttcaa
    13441 agtggggaac ttgctgtctt ttggattctg tttatactgt ttctgattgg ctttttataa
    13501 tatttattgc tttaacctta attatttttg gtatagcagg ttatatcttt ttgttttctg
    13561 gcggtgatcc acaaaaaaat agtaaagccc aaaaatttct tatttatggc gcagttgcga
    13621 ttgcccttgt tgctttgtca aaaataattc cagcaattat caaggcaatt gttgcctaaa
    13681 aaaaggtcaa aaaaataatg atgagaaagt attgattttt attaattttt agattataat
    13741 attataataa agaaaggttt attgcatttt gcaatataaa aggtcggtgt ttctaataat
    13801 tttaaaacgt tattatgatg aaaaaatttt ttgcagtcat actaacccta gtaatggttt
    13861 ctttgatcgc agcgccaatg atagcatcag ctcaaaccgg tgccaaggag tgttgtcagg
    13921 taaaaagaaa ttatactgat ttagatggaa attgtgggaa aggtaaaata gttggtcctg
    13981 caggtggatc ctgcgatatt ggtgatatta attgttcaag taatgactgg ggcaccattt
    14041 gtttaatgga aactgtttat aatgttactg actggctttt ctatattgtt ttatcaatag
    14101 cagtaatctt tggagtaata tctggcttta tgtttatgat atctagtgga gatcctacca
    14161 agaccgagaa agcaagaaat ctcttaatgt atatggctat tggtttggtg gtagcagctc
    14221 tggcaaaggt tattccttcc attgttagaa caatggtcgg tgtctaacat tagcacttag
    14281 caatcctaat aaaaagagca atctttgaaa gattgctctt ttgttgtttt gtgccgcggg
    14341 agagattcga actctcatag gtttccccat tgggtcctaa gcccaacgcg tctgccagtt
    14401 ccgccaccgc ggcttttatc agaaatattg ttaaaattat tcagcgtttg tagtggggta
    14461 taagttaggg gatttataaa atgagagaaa tgttttttta agaattagtt ctccattttt
    14521 ccaaacttct tcctctaatc gcgctttcat tgatccatct tcttgaaaat catattgata
    14581 tggtcctttt acaataactt ttctaccgtc gtctttgcca taaaattcaa aggtgagatt
    14641 attgtctttg atttttgctt gaatcaatat ccaatttcca gtattgtttt tgaacttaag
    14701 gtcgggcgag ggtagataca cggttgcatc aaatccttgc ggattgtagt atttaacagg
    14761 gtaggcatgg gattttcttt ctgtgatttc aagtcctgaa tagaccgctg ccctaaacat
    14821 tgtagttgaa acctgacata ccccgcctcc gtattcggga acagttttgt tgttttttat
    14881 aaccaattct ggtagatatc cttgagctgg tccaacttct cccagggctt gattaaaaga
    14941 aaattcttca tccggtgcaa ttaacattcc attgaacttg gctgtagcga ttttaatatt
    15001 atgtattctg tttttaggtg aaccagcaaa gtttgaggtg ccagtgccga ttaatttttc
    15061 aatatggagt tcattaattt tttcttctgt aatttttggt tctacttctt caaatcttaa
    15121 tgaaatttgt ttttcttttg ctaaaatatt ttcttgtatt tttttagcac tttcttctat
    15181 ttttaaaatt tttccggtct tgggagcaat aataattttt aaaacacctt cttcgaaact
    15241 taaaacagga tttcgagctt ctatattaat actattagag atttcagcta gataattttg
    15301 aatcagttct tcattaagag aaggggacag agtgatttga ttgtctttct tttgagggat
    15361 aaatgaaaac caatttccca gtgttttttg aggaacctca atgcgttttt cttctgccaa
    15421 aagcacatag ggccctgctt tcaagatctc ttttgcttga ttcgctgcac ttaaagccat
    15481 ttcttgagtg atttcaggag atgcgtcttc taggactaga taaatattgc ttattatcaa
    15541 aaaacctgcg tttttttcaa tttctttttt tattttttct cttgaaaaaa gaaggccttc
    15601 ttttggttct tcgatatcaa aatccaaggt tttttcatta aatatcacta cggcgttttg
    15661 aggtggaatt tcaaacttgc caaagttttc agtaaaaaac ttttcaaatc cttcctcttt
    15721 ttcttctttg ccatcgattg ttgctatcaa agagaattga taattccctt tgagggaggc
    15781 aattttttct tttgtatttt gaaagaaatc attaatgctg ctatttgacg aaaattttct
    15841 tccgactgaa aacggttctt ctaaactttt ttcaatatca aaaacaatac ccaaatcttt
    15901 tatttttatt tcttttgagt aaattccttc agaattagaa aaatgaaaag taatatcttt
    15961 ttctaaaaat tcatttacct ttgtttcgat gagattttcg gcttgggtct ttgataatcc
    16021 tccaatcttt tctcctccta ctgtaattcc acgagcaaaa gaatcctttg gaagatataa
    16081 aatataaaaa gcaactgcaa taacaacaat ggctgttatg caaaaaatca ataagccctt
    16141 tttcatagcg tttacaaatc aagaatatta attattttac ctctgtgacc cttatttttg
    16201 tagttttagt tttttcgagt tttggcagtc gcagggttaa aatgccattt ttaagttctg
    16261 ctttggcttt actgggatct atttcttcag gaagaataat ttgtcgagaa aatgcccccc
    16321 aataacattc ctgatgaaag tagtcttgag gatttacttc caattctttt ttcctttcac
    16381 ccctgatcgt gaccatatcg ttttgaatac taatatcaag atcttcgctt tttactccgg
    16441 caatagtaga ttgaataacc agatcactat ctgtttggaa tacatctact gccaattgtc
    16501 cttcctcgct attctctgtt tctcttgtag agggtccatt ttgaacacca agattttcat
    16561 tgtttattcc tccttcattg ttggataatt tttcaaaaaa atcactcata aatttatttt
    16621 taatttaaaa tattttgacc ttttcccctt cccagaaata atttttattt tcttttttca
    16681 ggggtattat atttttgatc ctcttggcaa acatttcact tttttaaaaa agatataaac
    16741 aataattgcc attaagaata ttataacaat tggaagtaaa aaagaaaaga ggttctcagc
    16801 actgtttagt tctataatag agagattgta aagtccgtga aaagtggctg caattaaaat
    16861 tcccacagca ataaagattt tttttctgtt ataaagggat actgccaaaa aataacctgt
    16921 aatcgctgca gtcaaactgt gcaaaaagac agctgttaaa agacggatta aacttacaaa
    16981 aattgcttct tctagtgctg cactgcttag tattaccgag agattttcaa ccatagcaaa
    17041 tcctaaacca gaaataatca aatagagcat taaatcaatg ggttcatcaa gatgagatga
    17101 attgatgaca cgttttttaa caacccaata tttaaagaat tcttctacaa ctcccacaac
    17161 aaaaatataa taaataaaga ggaggattgt attttgcaat aatatatctt tttctggaaa
    17221 aaaacattcc aaaatagttt ctgcaagata aacaggcaca accattattc ctccccaaag
    17281 aaaaatttct attatttttt tattagattc tggatgaaca tcttgacgca aatagaaaag
    17341 aagccataca attgctggta aggcgccaat tacaaaatat aaaataaaag tagatataga
    17401 catattaaca ttttaacata taaaatattt aggttcaaaa atttaacgca acactcttta
    17461 attaatttct aatttctaat ttctaatttc taaaccctta aatccgtatt cgt
//
LOCUS       DAOTQD010000008        16969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_3372_length_16969_cov_7.339778, whole genome shotgun sequence.
ACCESSION   DAOTQD010000008 DAOTQD010000000
VERSION     DAOTQD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16969
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3372_length_16969_cov_7.339778"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="leuS"
                     /locus_tag="P5570_00705"
     CDS             complement(<1..1324)
                     /gene="leuS"
                     /locus_tag="P5570_00705"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRR39267.1"
                     /translation="MKKKCNHKKFNQDKNKQKIKVKTNSEWEEIHCQIEKKWQQIWKN
                     QGTFKAKNTSKKPKFYCLDMFPYPSAYGLHVGHLKGYTFSDLITKKKKMEGFNVLHPM
                     GFDAFGLPAENFAIKTGIHPAITTKKAISNIKKQLVFAGMGYDWEREIITCKPEYYKW
                     TQWMFLQLYKAGLAYKKKAPVNFCPSCKTVLANEQVIEEKCERCSSIVEKRYLEQWFL
                     KITDYAERLLKDLDKIDWPEKIKIMQKNWIGKSEGTEISFQLTKNNLQLKVFTTRTDT
                     IFGCTYVVIAPEHPLIEQCKSKIENFNEIQNYIQKSKNKSEIERTSESKTKTGIKIKG
                     IKAINPVNKKEIPIFVADYVLMEYGTGAVMGVPAHDERDFAFADRYQLPIKTVIVPRD
                     KSKIKNIKISKNGAFIEDGILINSGIFTGLSSNEARQAITQYLAKIGKA"
     gene            complement(1343..2311)
                     /locus_tag="P5570_00710"
     CDS             complement(1343..2311)
                     /locus_tag="P5570_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRR39268.1"
                     /translation="MQNKSKKIKWSVFFILIAILIIFVFDFENRNQTEQQNNNQDVNQ
                     DVNQDKIENPLLTMREVILPTPLILSEKQNEIDALEKEKILFWTNVYRINNNVQELTE
                     NEILNQAAQQKIKDMFEKQYFEHISPEGIGAADIVEGLSYKYLMVGENLALGNFKDEK
                     DLVDAWMASQGHRENILNSKFKEIGIAADRGIFNGERTFLAVQIFATPLSKCPLPDEE
                     LKKSIEEKEKELDNLQNEINLLEDEIESLKKEQEMVYNQGKDLVINEEIEAANQRLKE
                     LNDKINLKIQKYNQSIIEFNNLTQEVEDLISRYNLQVNNFNQCLTQ"
     gene            complement(2316..4793)
                     /locus_tag="P5570_00715"
     CDS             complement(2316..4793)
                     /locus_tag="P5570_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRR39269.1"
                     /translation="MFNPSSAPIFKTINLSRVFPLGIVRFFEIFFFLLFVVSLISIQV
                     YSIFDYHLSLLIAYWSFLLWGVFFLYRCFFNQKLKNPEIKNPENLAEFLDFESAEAVN
                     FALTKKGDFTMLLLFYLASLKKLSFVFERLGLDQKEMQKELKKRLKGTKKDTEEIEET
                     LSVILKETWRKITIFDLFGALVETEPLFKKFLDPLQLSRKDVKEVCRWQESILEREMK
                     EKRFWEMENLLKHSALFQDWAAGYTITLDNFSIDITKMEKPNLLPSFFLHKKELEKLE
                     NALIKSERSCALLVGEVGVGRKEIIKNFARKINQGKSLPSLNYQRVVEIDMASVLSAG
                     REMGDIEQFIRIIFDEAIRAGNVILVIRQIHNFIGFEFGAESIGKIDISSVLNQYLSI
                     PGFRLIGITTYEGFHRAIEKVPELQGQFEKIEVNPPSKEETLRIVEDEILKIEKRAQV
                     FFPFRSIKEIVELSDKYIQTIPFPKKAIDLLEELIIYALRIARTRKIQPEVVEAFISE
                     KTEIPVGKVAQQEKEILLNLENLIHQRLVNQEEAVSEIANALRRARAGIQERKKPIGN
                     FLFLGPTGVGKTETAKCLARIYFGSEKRIIRLDMSEYQTLSSISRLIGTAREPGYFTT
                     QIREDPFSLVLLDEIEKSHPSILNLFLQVLDEGFLTDGAGKTVDFKNTIIIATSNAGA
                     ELIRQAVLKGKDLMEYKEEFINEILMGGIFKPEFLNRFDAVVLYKPLSREHFQKIAAL
                     MIKDIQNGLLEKNIEFSFDSELVVKIAEIGYNPEFGAREMKRVVQNKLENNIAKAILA
                     GEIKEGDKITIDPANFEVKKIKKEIVF"
     gene            complement(4835..5224)
                     /locus_tag="P5570_00720"
     CDS             complement(4835..5224)
                     /locus_tag="P5570_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39270.1"
                     /translation="MMQNISLFFHWYFIEIPKKIYRIWSNYLWFFKNYFSITELIKSF
                     FSPWKRIYFEKKTKGIDLEEWFGNFIFNTFSRIIGIILRSFAIVIGIISELFAFVAGI
                     VCFVLWLIFPFLLIFCLAQGINNIVKL"
     assembly_gap    5795..5894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5986..6855)
                     /locus_tag="P5570_00725"
     CDS             complement(5986..6855)
                     /locus_tag="P5570_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39271.1"
                     /translation="MSGFVRQQEKKRKKGGLNMMTEVNLEELIEKGIVEDLFKKARKI
                     YLDEEKKYINRNHLRHKIESSIISWYRETSMPAKVYTCYGGIKITAEPLWTIFLEPLI
                     NESFFSFSIVPKEVILEGKGFYPKEVKYSVLGNLKTGEMYFSYSDHGAYYKKIYYSNE
                     KKYLPLEKKDLNNGNNPIIKIGASNHLGQRKHREKKIDKKRCKEVQLLLNQEGFVKSE
                     NPIIVDMINTIEGKIDGYIYHRKGIKNWLAFYLLAKETNHRLFINGKDIENKKIRNFF
                     KPSAVFLIKEDLL"
     gene            complement(6994..7596)
                     /locus_tag="P5570_00730"
     CDS             complement(6994..7596)
                     /locus_tag="P5570_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39272.1"
                     /translation="MNFPFEVRRKFIHIVLGILFSILIYFDLFSLPFWGGILFLGIIF
                     SFIFKKYQLPYISSFILAFERKEEIDHFPLRGALTFLFGSILSYIIFPKFIAISAIIT
                     LFLGDSIACLYGIYFGRIKAPWSPKKHLDSAMIGALCSAFLIALFFPLWKGFLAALTA
                     IFFESIVDFGKLPNNLIRLIFDDNVFIPLISGGVLFLLNL"
     gene            complement(7602..8372)
                     /locus_tag="P5570_00735"
     CDS             complement(7602..8372)
                     /locus_tag="P5570_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR39273.1"
                     /translation="MEKTQEENIKQEENKKQKKHHIDPWMITSVVLFVLIIGSIFTDG
                     FGIKNLLKSSDKIAQKTLEFINQNMLAEGYTASLVESRYLNNGLYEVKFQVEDQEFDS
                     YVSGDGKMLFLQGINMEEVTAENEDQSDETTVTGGFTETEDEICTENGKPIVYFFGSN
                     SCPHCQWEEPVLKEVVSKFGDKIVFHKNIDSSDDQEIFDKYSTGGVPTIVLGCKYYRV
                     GSGENDTKEVESQALTDLICDLTEDQPSDVCQVKAEQE"
     gene            complement(8436..8795)
                     /locus_tag="P5570_00740"
     CDS             complement(8436..8795)
                     /locus_tag="P5570_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRR39274.1"
                     /translation="MAKETKQGAGKKVAAGLLAGAIAGMIAGILVAPKKGEETRKELK
                     NRLEKNKFAKEVAKRLEKMGEVTKEKYNQAVDEVSDIYRRAKKVKDEDLKEIVEEIRD
                     RWPEIVKKIKTPTKQKK"
     gene            complement(8838..9101)
                     /locus_tag="P5570_00745"
     CDS             complement(8838..9101)
                     /locus_tag="P5570_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39275.1"
                     /translation="MIEETVFYILASVFLGLAIIFMCLVLFYWIRTLRNFQKISSNLK
                     ETSEELKEKITSFSGIAAGMAVLIKKILESQSKKKNKKGQKNL"
     gene            complement(9164..10114)
                     /locus_tag="P5570_00750"
     CDS             complement(9164..10114)
                     /locus_tag="P5570_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HRR39276.1"
                     /translation="MTIKEIHNLGIKMGIEADFRGKDGVERFLKRKQEKYNDLPEKIK
                     KDFDKEALENPFLDSRILHIANDKEIKKILVGIDIEVAEILLAKEIGNIDLIIGHHPL
                     GKALSSLSDVMELQCDVLNTYGVPINIAEGLMKERIEEVSRGLNGLNNQRTVDAARIL
                     GFNLMCLHTACDNLAAQFLKEQIEKNKFETVQELMNFLKKIPEYKEAIKLGLGPRIFA
                     GSPDRRCGKIAITEITGGTEPTSKIYEKMAQAGIGTIVGMHISEESRKEAEASHLNIV
                     IAGHMSSDSLGVNLFLDELEKERIEIIPCSGLIRISRQKF"
     gene            complement(10156..11055)
                     /locus_tag="P5570_00755"
     CDS             complement(10156..11055)
                     /locus_tag="P5570_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRR39277.1"
                     /translation="MSLYSQAEANTQKTFLLIIGFCILLIGFGWLFSYIFDNYIILII
                     ATVLAICQSFFSYWYSDKIVLSTTKAKPIEKKDNPELYRIIENLSITAGLPMPKIYIL
                     NESQPNAFATGRDKNHAVIVVTQGLLEKLERSEIEGVIAHELSHIGNKDILLSTVVVI
                     LVGIIAILSDFFLRISFWGGRRSSSDESKSGIIMFALGILAAILAPIAASLLQLAISR
                     KREFLADATGALLTRYPEGLARALEKISDDPSPLKTANTSNAHLFISSPFKGNQSKNW
                     LVKLFSTHPPVEERIKALREMTI"
     gene            complement(11068..11691)
                     /locus_tag="P5570_00760"
     CDS             complement(11068..11691)
                     /locus_tag="P5570_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRR39278.1"
                     /translation="MDIWNTLIIILIILLFVSGFFGYIEWNINIPLIIVLIIFGGISI
                     YNELVKLRNRAKEAWADIDVQLKRRYDLIPNLVETVKGYAIHERELLEKVTQARSKAM
                     EAKDLKEKGEAENILSGTLKSLFAVSENYPNLKASENFLELQRELRDTEDKIQAARRF
                     YNGVVRDLNTRIESFPANIIASMFKFKPMEMFEIEKKEEKEPVKVSF"
     gene            complement(11782..13209)
                     /locus_tag="P5570_00765"
     CDS             complement(11782..13209)
                     /locus_tag="P5570_00765"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRR39279.1"
                     /translation="MEIKNLRKINDYQWEIPKTGKMLVPGIIFGDQKLVKEMDEKVKE
                     QVQNVACLPGILKASLAMPDCHWGYGLPIGGVAAMDKDEGVISVGGIGFDSGCGLRTL
                     KTDLTFTDVKPKVKELVDFLFETVPCGLGSRGKIILSKKETDDLLKEGTKWVLKNGYG
                     VKDDLEYIEDNGSFEGANPDYVSERAKKRERGQVGTLGSGNHYEEIQYIDEIYDKEAA
                     QKFGLFKNQILVSIHCGSRALGHQIGTDYLKVLAKASRKYHIPINDRELVCAPINSKE
                     GQRYFMANLCALNYSYANRQVIVHLTRECFKKIFPGAKVETLWDLTHNSARWEKHEIN
                     GEFKKVLVHRKGATRAFGPGREELPENYRIVGQPVLIGGTMGTCSFVLKGTEVGMNLA
                     FGSASHGAGRAMSRTKAKKIWWGQSLLEELKQKGIYARAASFSGFAEEAPGAYKDVTQ
                     VIEAVDKAGLAKKVVRMKPLGVIKG"
     gene            complement(13264..13692)
                     /locus_tag="P5570_00770"
     CDS             complement(13264..13692)
                     /locus_tag="P5570_00770"
                     /inference="COORDINATES: protein motif:HMM:NF001617.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HRR39280.1"
                     /translation="MKTYEQFEHKADVGVRGYGKTIEEAFENGAKAMFSVMVNLKKIE
                     PKKEIKIECKADNLEELFIEWLNTLLSEAGIENLVFGEFKIKEIKKTKSGYKLAGVVQ
                     GEELNPEKHEAEVEVKAATYSQLKIERKGNQYIAQTIVDV"
     gene            complement(13679..14314)
                     /locus_tag="P5570_00775"
     CDS             complement(13679..14314)
                     /locus_tag="P5570_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRR39281.1"
                     /translation="MKLIFDFDHTLFSVKRFYNTLENNFQKIGINKKLLKETFEKAAK
                     EKPYNPEKQFQLIIEHNPEVSLKLLRENFKNVLHRANTFLYPDVENFFKKTEKNIDLF
                     LVSYGDKKFQRAKIENTGIVQFFEKIIITSDINKVSVLKKLLKKNEKAIFVDDNPETL
                     SAVKKEFPQIITIRMKRREGKYQKEKDNPNIDFSINNLKELEKILFQYENI"
     gene            complement(14602..15855)
                     /locus_tag="P5570_00780"
     CDS             complement(14602..15855)
                     /locus_tag="P5570_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRR39282.1"
                     /translation="MLQKFLPTQAVILAAGESSRFWPFNQKHKSLFKIMGKPLIWYTI
                     NGLKKSGIKEVIIVEGKAKDIEKELKNYRFQNLKIRYVIQPKPIGMGDALWRTKDLLK
                     DKFLVLNAERVDVNEILNLRFPNFNSKIKGILFGQKTRNPQLYGMAKLKGNRVLEIVE
                     KPQPGKEPSDIKIVGVYLLEPGFFEFYKKAKKGMYNFEETLSLYMIKNDTRIVILNEP
                     EEETPTLKYPWHLFLMEKYLMDKFLKSIIAENAKIGKNVVIEGKVQIENNVKILEGAV
                     IKGPCYIGENCVIGNNSIVREYVNLENNVLIGALAEVARSIFEEGSHTHSGFFGDSIF
                     GENCKIGAGTVTANKRLDRKEISTLVKGEKISTQLKRLGVVIGENSNIGINVSLMPGV
                     FIGVNCNVGPSLVVKKNLKDRTFLF"
     gene            complement(15862..16839)
                     /locus_tag="P5570_00785"
     CDS             complement(15862..16839)
                     /locus_tag="P5570_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRR39283.1"
                     /translation="MRKKKIVCFGGGGAMPQLILEPLKNFNFDIVSITSMVDNGGSTG
                     ALRKEFNTMPAGDIRRHLLALSEGEKWKKELWQFRFAKDIEISPQHFGHNFANVFITG
                     LESIFGNFEEALNICHQFLNVRGMALPAILDKVQLVAELEDGSLIEGEEEIDLGKNHN
                     RDLKIKNVYLKPKARAYQKAIDEIITADNIIIGPGDLYSSLIPCFLGKGWKEAMAQSH
                     AKKIFICPAMTKWGETQNYSIKDFTEAIEKYLGVPLDFVIFNNNIPSKDTIKKYQEEK
                     KEEFVLNPPIINNDLDKNKFIGEDLLLDGLEIKYDQQKLFSFLKKIINF"
BASE COUNT         5306 a         3111 c         2121 g         6331 t
ORIGIN      
        1 ttgctttgcc tatttttgct aaatactgag tgattgcttg ccgtgcttca tttgaagaca
       61 aacctgtaaa tattccagaa ttaataagta taccatcttc aataaaggct ccatttttac
      121 taatttttat gttttttatt tttgatttat ctcgaggaac aattactgtt ttaatgggca
      181 actgataacg atcagcaaaa gcaaaatctc tttcatcatg tgcgggtacg cccattactg
      241 ctcctgttcc atattccatt aaaacataat cagcaacaaa aattggaatt tcttttttgt
      301 taacaggatt aatagctttt attcctttta tttttattcc tgtttttgtt ttactttcag
      361 aggttctttc aatttctgat ttattctttg atttttgaat ataattttgt atttcgttaa
      421 aattttcaat ttttgactta cactgttcaa taagcggatg ttctggtgca ataactacat
      481 aagtgcaacc aaaaattgta tctgttcgag tagtaaatac cttaagttgt aaattatttt
      541 ttgttaattg aaaacttatt tctgttcctt cactttttcc tatccaattt ttttgcatta
      601 tttttatttt ttctggccaa tcaattttat caaggtcttt taataatctt tcagcgtaat
      661 cagtaatctt taaaaaccac tgttccaaat agcgtttttc cactatgctt gaacatcttt
      721 cacatttttc ttcaatcacc tgttcatttg ccaatactgt tttacaagac ggacaaaaat
      781 tcacaggtgc ttttttctta taagcaagtc cagctttata gagctgtaaa aacatccatt
      841 gagtccattt ataatattct ggtttacagg taataatttc tctttcccaa tcatatccca
      901 ttccagcaaa aactaattgt tttttaatat tagaaattgc cttttttgtg gtaatggcag
      961 ggtgaattcc tgttttaata gcaaaatttt ctgcagggag accaaaagca tcaaatccca
     1021 ttggatgcaa tacattaaac ccttccattt ttttcttctt tgtaataaga tcagaaaatg
     1081 tatacccctt taaatgacct acgtgtaatc cataagcaga tggataagga aacatatcca
     1141 aacaataaaa cttcggcttt tttgatgtat ttttagcttt aaatgttcct tgatttttcc
     1201 aaatctgctg ccattttttt tctatttggc aatgaatttc ttcccattca gaatttgtct
     1261 taacttttat tttttgctta tttttatctt gattaaactt tttatgatta cattttttct
     1321 tcataattga taagaaatat tcctattgag taaggcattg attaaaatta ttcacctgta
     1381 aattataacg agaaattaaa tcttccactt cttgggtaag attattaaat tctataatcg
     1441 attgattata tttttgtatt ttcaaattga ttttatcgtt taactctttt aacctctgat
     1501 tagcagcctc aatttcttca tttattacta aatccttacc ttgattatac accatttctt
     1561 gttctttttt aaggctctct atttcatctt ctaaaagatt tatttcattt tgaagattat
     1621 ctaattcttt ttctttttcc tcaatacttt ttttaagctc ttcgtcaggt aatggacatt
     1681 ttgataaagg agtagcaaaa atttgaactg ctaaaaaagt tctttcacca ttaaaaatgc
     1741 ctcgatcggc agcaatacca atttctttga attttgaatt taaaatattt tctctatggc
     1801 cctgacttgc catccaagca tcaactaaat ctttttcatc tttaaaattt ccaagagcaa
     1861 gattttcgcc taccattaaa tatttataag aaagcccctc aacaatatca gctgcgccta
     1921 tgccttctgg tgaaatatgc tcaaaatatt gtttttcaaa catatctttt attttttgct
     1981 gagcagcttg atttaaaatt tcattttctg taagttcttg aacattgtta tttattctat
     2041 aaacattagt ccagaataaa attttttctt tttctagtgc atcaatttca ttttgttttt
     2101 ctgataaaat taaaggggta ggtaaaataa cctctctcat tgttaataac ggattttcta
     2161 ttttatcttg attcacatct tgattcacat cttgattgtt gttttgctgt tctgtctggt
     2221 ttctattttc aaaatcaaag acaaaaataa ttaaaattgc aatcaaaata aaaaaaacac
     2281 tccatttaat cttttttgat ttattttgca taaagctaaa aaacaatttc tttttttatc
     2341 ttttttactt caaaattagc aggatcaatg gtaattttgt ccccttcttt aatttcacca
     2401 gcaaggattg ctttggcaat attattttct aatttatttt gaaccacacg tttcatttct
     2461 cttgctccaa actcagggtt atatcctatc tcagcaattt ttacaaccaa ttctgaatca
     2521 aaagaaaatt caatattttt ttctagaaga ccattttgaa tatcttttat cattagtgca
     2581 gcaattttct gaaaatgttc tctggaaagg ggcttataaa gaacaacagc atcaaatcta
     2641 tttaaaaatt ctggtttaaa aattcctccc attaaaattt cattaataaa ttcttctttg
     2701 tattccatta ggtccttacc ttttaaaacc gcttgtcgaa ttaactcggc tccggcatta
     2761 gaagttgcaa taataatggt atttttaaaa tctactgttt tacctgcacc atcagttaaa
     2821 aatccttcat ctaaaacctg taaaaataaa tttaaaatac taggatgtga tttttctatt
     2881 tcatcaagca acaccaatga aaatggatct tctcttattt gcgtggtaaa gtatcctggc
     2941 tcacgagcag taccaattaa acgactaata gagcttaatg tttgatattc tgacatatca
     3001 agtctaatta ttcttttctc tgagccaaaa tagatacgtg caagacactt tgctgtttca
     3061 gtttttccta caccagtggg gccaagaaat aaaaaatttc caataggttt ttttctttct
     3121 tgaatacctg ctcgcgctct tctaagggca tttgcaattt ctgaaactgc ttcttcctga
     3181 ttgaccaacc gctgatgaat aagattctcc aggtttaaaa gaatttcctt ttcttgttga
     3241 gccaccttgc ctactggaat ttctgtcttc tctgaaataa acgcctctac cacctctggc
     3301 tgaatttttc tggttctggc aattcttaaa gcatagatta ttaattcttc caagagatca
     3361 attgcttttt taggaaaagg tattgtttga atatatttat cagataattc aacaatctct
     3421 tttattgagc gaaatggaaa aaatacctgt gctctttttt caatttttaa aatttcatct
     3481 tcaacaattc ttaaagtttc ttctttagac ggagggttta cttcaatttt ttcaaactgg
     3541 ccttgaagtt caggtacctt ttcaatagca cgatgaaacc cctcataagt agtaattcct
     3601 attaacctaa atccaggtat agaaagatac tgatttaaaa cagaagaaat atcaattttt
     3661 cctatacttt ctgccccaaa ctcaaaacca ataaaattat gaatttgacg aataaccaaa
     3721 attacatttc cagcacgaat tgcttcatca aatataattc ttataaattg ttctatatca
     3781 cccatctctc ttcccgcaga aagcacagaa gccatatcaa tttctaccac tcgttgataa
     3841 tttagagaag gtaaactctt gccttgatta atttttctgg caaagttttt tattatttcc
     3901 ttgcgcccca caccaacctc tcctaccagc aaagcacaac ttctctctga ttttattaaa
     3961 gcattttcta atttttcaag ttcttttttg tgaaggaaaa acgaaggtaa aagattgggc
     4021 ttttccattt ttgtaatgtc aattgaaaaa ttgtctaagg taatagtata tccagcagcc
     4081 caatcctgaa aaagagcact gtgttttaaa agattttcca tttcccaaaa tcttttctct
     4141 ttcatttccc tttctaaaat actttcctgc catcgacaaa cttcttttac atcttttcga
     4201 gaaagttgaa gagggtcaag aaacttttta aatagcggct ctgtctcaac taacgctccg
     4261 aatagatcaa aaatggtaat ttttcgccat gtttctttta gtatcacact taatgtttct
     4321 tcaatttcct cagtgtcttt tttggtgccc tttaatcttt tttttaactc tttttgcatt
     4381 tctttttggt ctagtcccaa tcgttcaaat acaaatgata atttttttaa tgatgccaaa
     4441 taaaaaagca aaagcattgt aaaatccccc ttttttgtca aagcaaaatt aacagcctca
     4501 gctgattcaa agtccaagaa ttcagctagg ttctctgggt tttttatttc cgggtttttt
     4561 aatttttgat taaaaaaaca acggtagaga aaaaacactc cccatagaag aaaagaccaa
     4621 taagcaataa gcaatgataa atgataatca aaaattgaat aaacctgaat gctgattaaa
     4681 gagacaacaa ataaaaggaa aaaaaatatt tcaaaaaacc ttacaattcc cagaggaaaa
     4741 actcttgata aatttattgt tttaaaaata ggagctgatg agggattaaa catattttat
     4801 ttttatgaat atattttttt actttcaact tttattataa ttttacaata ttattaattc
     4861 cctgtgccaa acaaaaaatt aaaaggaacg gaaaaattaa ccataaaaca aaacaaacta
     4921 tacctgctac aaaagcaaat aactcgctaa ttataccaat aacaattgca aacgacctca
     4981 atatgattcc aatgattctt gaaaaagtat taaaaataaa attaccaaac cattcttcta
     5041 agtcaattcc ctttgtcttt ttttcaaaat aaattctttt ccagggggaa aagaatgatt
     5101 ttatcaattc tgtaattgaa aaatagtttt taaaaaacca aagataattt gaccaaatac
     5161 gataaatctt tttgggaatt tcaataaaat accaatgaaa aaaaagagaa atattttgca
     5221 tcataatatt tttataaatt cgtatctatc cttaaggata tcaaatcctg attataatgt
     5281 aaaggaattt ttaaatactg tcgctccaat atgataattt catagttgag caatttagaa
     5341 atgtcatata gttaattata aattaaccta aaatctaaga aaaaactatg gcagtactaa
     5401 ttactatgac tcaaaaagag ttatcaaggt atgagatata agaattttaa atttttgaca
     5461 aaataaacaa attattatac tatattaatg attaatgatt tttaaaaaac aaaggaggaa
     5521 cgttttggag tagccctttt gtttgctttt ttagcaaaca gtagggctac cctaaggcgt
     5581 tgacgttgtt cgggccaaaa aaggagccaa aacgtaaagg aggtgaaaaa ttatagacat
     5641 cataagagca gcaatattaa ttcttttaat ttctatcttt tcttttttag ttttatcctt
     5701 cttttttaaa atagaaccaa aaaaggaaag aaaatttaaa agatttttaa ttattttttt
     5761 tattatttta attgctgtcc tggtgtattt ttaannnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnaagatt tttaattatt ttttttatta ttttaattgc tgtcctggtg
     5941 tatttttaaa taaaaagggt aatagattat attaccctta tttttttata atagatcctc
     6001 cttaatcaaa aaaactgctg atggtttaaa aaaattccta atttttttat tttctatatc
     6061 tttgccgttt ataaaaagac ggtgatttgt ttcttttgcc agcaaataaa aagcgagcca
     6121 attctttatt ccttttctat gataaatata accgtcaatc tttccttcta ttgtgttgat
     6181 catatcaaca attattggat tctcactttt aacaaatcct tcctgattta aaagtaactg
     6241 tacttcttta catctttttt tgtctatctt tttttcccgg tgctttcttt gtcccaggtg
     6301 attgctagca cctattttaa taatggggtt atttccatta ttaaggtcct ttttttccaa
     6361 gggtagatat tttttttcat tgctgtaata tattttttta taataggcgc cgtgatcact
     6421 gtatgaaaaa tacatttctc ctgttttaag atttcctaga acactgtatt taacttcttt
     6481 aggataaaat ccttttcctt ccaaaataac ttcttttgga acaatactaa atgaaaaaaa
     6541 cgattcgtta attaagggtt ctaaaaaaat agtccaaagc ggctcagctg taatttttat
     6601 accgccatag caagtataaa cttttgctgg catacttgtt tccctgtacc aggaaattat
     6661 actactttct atcttatgac gaagatggtt tctgttaata tatttttttt cttcgtcaag
     6721 ataaattttt cttgcctttt taaaaagatc ctctacgatt cctttttcaa ttagttcttc
     6781 aaggttcacc tctgtcatca tgttaagtcc tcctttcttt ctttttttct cctgttgcct
     6841 tacaaagcca gacacggctt caatttctct tctttctttc tttcaatcac cgtttcaggt
     6901 ctcaacccca aacctgaatt gttaaagtac acctccttta attaattttt tgggttgatt
     6961 aatattaata gcaataaata aaaaatttgt caattaaaga tttaataaaa acaaaacacc
     7021 accgctaatt aagggaataa atacattatc atcaaaaatt aatctaataa gattatttgg
     7081 cagtttccca aaatcaacaa tgctttcaaa gaatatcgct gttaatgccg ctaaaaatcc
     7141 tttccacaga ggaaagaata aagcaattaa aaatgccgag caaagagcgc ctatcatagc
     7201 gctgtctaag tgtttcttgg gagaccaggg ggctttgatt cttccaaaat atattccgta
     7261 aagacaggca atcgaatcac ctaaaaataa agtaataatt gctgagatag ctataaactt
     7321 tggaaaaata atataactta agatagagcc aaaaagaaaa gtaagcgctc ctcgtaaagg
     7381 aaaatggtca atttcctctt ttctttcaaa agcaagaata aacgatgaaa tataaggaag
     7441 ttgatatttt ttaaaaataa aagaaaaaat aattcctaaa aataaaatcc ctccccaaaa
     7501 aggaagggaa aataaatcaa aataaatcaa aatggaaaat aaaattccca aaacaatatg
     7561 aataaatttt cggcgaactt caaaaggaaa attcatctta tttattcctg ctctgctttt
     7621 acctgacaca catcgctagg ttgatcttca gtaagatcac atatcaaatc agtaagggct
     7681 tgagattcta cttctttagt atcgttttct cctgatccta ctctataata cttgcaaccc
     7741 aagacaatgg taggaactcc accagtgcta tatttatcaa aaatttcttg atcatcgctt
     7801 gaatcaatat ttttgtgaaa aacaattttg tccccaaatt ttgaaactac ttcttttaat
     7861 acaggttctt cccattgaca atgagggcaa ctgtttgagc caaaaaagta tactataggc
     7921 tttccattct ccgtacatat ttcgtcttct gtctcagtaa aaccaccagt cacagttgtt
     7981 tcgtctgact gatcctcgtt ttcagcagtc acctcttcca tatttattcc ctgtaaaaac
     8041 aacatttttc catcaccaga aacatatgaa tcaaattctt gatcttcaac ttgaaacttt
     8101 acctcataaa gaccgttatt aaggtatcga ctttcaacca gtgaagctgt atagccctct
     8161 gctaacatat tttggttaat aaattccaat gttttttgag caattttgtc tgagcttttt
     8221 aacaaatttt ttattccaaa accatcggta aatattgatc cgattataag cacaaataaa
     8281 acaacacttg tgatcatcca aggatcaata tgatgttttt tctgcttttt attttcttct
     8341 tgttttatgt tctcttcttg tgttttttcc ataaatttat cttccttgac aaacgtcaat
     8401 tagaagttct tactttttaa attcaattat tatatttatt ttttttgttt ggttggagtt
     8461 tttatttttt taacaatttc tggccatctg tctcttatct cttcaacaat ctcttttaag
     8521 tcttcatctt ttaccttttt tgctcttctg taaatatcag acacttcatc tactgcttga
     8581 ttgtactttt cttttgtaac ttcgcccatc ttttctaatc tttttgccac ttcttttgca
     8641 aacttatttt tttcgagtcg gttcttcagt tcttttcttg tctcttcacc ttttttggga
     8701 gcaaccaaaa tacctgctat cattccagca atagctccag ccaataatcc tgctgctact
     8761 ttttttcctg cgccttgttt tgtttctttt gccatagttt ttttaaacct aaaaatcaaa
     8821 tatgatttta ggtttgctta taaatttttt tgaccttttt tatttttctt ttttgattgt
     8881 gattctaata tttttttaat caatactgcc atacctgctg caatacctga aaaacttgtt
     8941 attttttctt tcaattcttc actcgtctct tttaaatttg aactaatttt ttgaaaattt
     9001 ctcaatgtcc ttatccaata aaacaacacc agacacataa aaataattgc tagtcctaga
     9061 aaaacagaag ccaaaatgta aaatactgtt tcttctatca taatttattt tctaataata
     9121 aatttagcaa aaatattttt aaaagtcaat agatcccgga gacctagaat ttttgacgag
     9181 aaattcttat caatccagaa caaggaataa tctctattct ttctttttct aattcatcta
     9241 aaaataaatt tactcccaaa ctatcactgc tcatatgacc agcaattaca atattaagat
     9301 gcgatgcttc tgcctctttg cgcgattctt cacttatgtg cataccaaca atagtaccaa
     9361 ttcctgcctg agccatcttc tcataaattt ttgaagtagg ttccgtgcca ccagtaattt
     9421 ctgtaatggc aatctttcca catctgcggt cagggcttcc agcaaaaatt cttggtccca
     9481 gacccaattt tattgcttct ttgtactcgg gaattttctt caaaaaattc atcagttctt
     9541 gtactgtttc aaacttattt ttttcaatct gctcttttaa aaattgagct gccaaattat
     9601 cacaagcagt atgcaagcac attaaattaa atcctaaaat cctcgcagcg tctactgtac
     9661 gctgattatt taatccatta agtcctcgtg aaacctcttc tattctttct ttcattaatc
     9721 cttctgcaat gttgatcggt acaccataag tatttaaaac atcacattgc aattccatta
     9781 catcagacaa gctacttaat gcttttccta aaggatgatg gccgataatt aaatctatat
     9841 tgccaatctc ctttgccaat agaatttcag ccacttcaat atcaatacca accaggattt
     9901 ttttaatttc tttatcattg gcaatatgta aaatccgaga atccaaaaaa gggttttcta
     9961 gagcttcctt atcaaaatct tttttaatct tttccggaag atcattgtat ttttcctgtt
    10021 ttcgtttcaa aaatcgttcc acgccatctt tccctctgaa atctgcttca atccccattt
    10081 ttatgcccaa attatgaatt tcttttattg tcataaataa tatgtgatta atgattttta
    10141 gtatatagat ttatattata tggtcatctc tcttaaggcc tttatccgtt cctcaactgg
    10201 tggatgagtt gaaaataatt ttaccaacca atttttggat tgattgcctt taaaaggaga
    10261 tgaaataaaa agatgagcat ttgatgtgtt ggctgttttt aatggcgatg gatcatctga
    10321 aattttttcc agcgcccttg ctaacccctc tgggtatctt gttaaaagcg ctccggtagc
    10381 atcggctaaa aactctctct tacgagaaat agcaagttgt aaaagagaag ctgcaattgg
    10441 cgccaagatc gccgctaaaa tacccaaggc aaacattatt ataccgctct ttgattcatc
    10501 tgaggaactt cttctccctc cccaaaaact aattcttaga aaaaaatctg aaagaatagc
    10561 tatgatgccg accaaaatta ctactactgt gcttaaaagt atatccttgt ttccaatatg
    10621 agaaagttca tgagcaataa caccttcaat ctctgatctc tctaattttt ccagcagtcc
    10681 ttgcgtaaca actattactg catgattttt atctctgcct gttgcaaagg cattgggctg
    10741 agattcattt aaaatataaa tctttggcat tggcaaacca gccgtaatag aaagattttc
    10801 aataatacgg taaagctcag gattatcttt tttttcgatt ggcttagctt ttgttgttga
    10861 aagaacaatc ttatctgaat accaataact aaagaaactt tgacagattg ctaaaacagt
    10921 tgcaataatc aaaataatat aattatcaaa aatataacta aaaagccagc caaagccaat
    10981 caaaagaata caaaatccta taatcaataa aaatgttttt tgtgtatttg cctcagcttg
    11041 tgaatacaaa ctcataaaaa attatattta aaaacttact ttcactggtt ctttttcttc
    11101 ctttttttcg atttcaaaca tttccattgg tttaaatttg aacattgaag caataatatt
    11161 tgcaggaaaa ctttcaattc tagtattaag atctctcacc actccattat aaaatcttct
    11221 tgctgcctga atcttgtctt cggtatcacg aagttctctt tgcaattcaa gaaaattttc
    11281 agatgctttc aggttaggat aattttctga aacagcaaat aacgacttta atgttccaga
    11341 aagaatattt tctgcttctc ctttttcttt taaatccttt gcttccattg cctttgatcg
    11401 tgcttgagtt actttttcta acagttctct ttcatgaatc gcatagccct ttactgtctc
    11461 aaccaaatta ggaattaaat catatcttct ttttaattga acatcaatat ctgcccaggc
    11521 ctcttttgcc cgattgcgta atttcaccag ttcattataa atactgatgc ctccaaaaat
    11581 aatcaaaaca ataataagcg gaatatttat attccattca atgtaaccaa aaaatccact
    11641 aacaaaaagc aaaatgatta atattataat aagcgtattc caaatatcca taattttata
    11701 aatttttatt tattaaattt tttcgacctt taaggggata atatttcaca ttatatcccc
    11761 tatcttttat tataacatat tttatccttt tattacaccc aagggcttca ttcgcacaac
    11821 tttcttggca agacctgctt tgtccactgc ttcaattact tgggttacat ctttataagc
    11881 accaggcgct tcttcagcaa aaccagagaa agaagcagct cttgcataaa ttcctttttg
    11941 ttttagctct tcaagtagag actggcccca ccaaattttt ttagcttttg tcctgctcat
    12001 tgctctgcca gcgccatgcg aagcgctgcc aaaggcaaga ttcataccta cttctgttcc
    12061 ttttaagaca aaagaacaag ttcccattgt ccctccaata agaactggtt gaccaactat
    12121 acgataattt tctggcaatt cttctcttcc tggaccaaaa gcccttgttg ctccttttcg
    12181 atgcaccaaa acttttttaa attctccatt gatctcatgc ttttcccaac gagcagaatt
    12241 atgggtcaga tcccaaaggg tttcaacctt cgctccagga aaaatttttt taaaacattc
    12301 tctggttaaa tgaacaatga cctgacgatt agcataagag taatttaaag cacagagatt
    12361 tgccataaaa tatctttgcc cttcttttga atttatcggt gcgcaaacta attctcgatc
    12421 attgatcgga atatgatatt ttcgagacgc tttagccaaa acttttaaat aatcagttcc
    12481 gatttgatga cccaatgccc gtgagccaca atgaatggaa accaaaattt gatttttaaa
    12541 aagaccaaat ttttgagctg cttccttgtc ataaatttca tctatatatt gaatttcctc
    12601 ataatgatta ccagaaccaa gtgtaccaac ctgacccctt tctctctttt ttgcccgttc
    12661 tgaaacgtaa tctggatttg ctccctcaaa cgatccattg tcttcaatat attccaaatc
    12721 atctttaaca ccatatccat tttttaaaac ccattttgtt ccttctttta aaagatcgtc
    12781 agtttctttt tttgataaaa taattttccc gcgcgatcct aaaccacaag gcacagtttc
    12841 aaaaagaaaa tctaccaact cttttacttt tggtttaaca tcagtaaaag ttaaatcagt
    12901 ttttaaagtt ctgagaccgc agcccgaatc aaatccaatt cctccaacac taattactcc
    12961 ttcgtcctta tccattgctg cgacccctcc aattggtaga ccatagcccc aatgacagtc
    13021 aggcattgca agagaggcct tgaggatccc tggcaaacaa gcaacatttt gcacttgttc
    13081 tttaactttt tcatccatct ctttaactaa tttttgatcg ccaaaaatta ttcccggcac
    13141 tagcattttt ccggtttttg gaatctccca ctgataatca tttattttac gaagattttt
    13201 tatttccata ttgaacaatt tttatacgat tgtataaaaa ttacgatgct ttatgcaaaa
    13261 aaattacaca tcaacaattg tttgagcaat atattgatta ccctttcttt caatttttaa
    13321 ttggctatag gtagcggctt tcacttcaac ttctgcctcg tgtttttctg ggtttagttc
    13381 ttctccctga actacgcctg caagcttata tccggattta gtttttttaa tttcctttat
    13441 tttaaattca ccaaatacaa gattttcaat tcccgcctct gataaaagag tatttagcca
    13501 ttcaataaaa agctcttcta aattatcggc tttacattca atttttattt cttttttggg
    13561 ctctattttt tttaaattta ccattacact aaacattgct ttggcgccat tttcaaaggc
    13621 ctcttcaatt gttttgccat aaccccttac gcctacatcg gccttatgct caaattgttc
    13681 atatgttttc atattgaaat aaaattttct ctagctcttt taaattgttt atagaaaaat
    13741 caatgtttgg attgtctttt tctttttgat attttccctc tctccgtttc attctaatag
    13801 ttattatttg aggaaattct tttttcaccg ctgataacgt ttccggatta tcatcaacaa
    13861 aaatcgcttt ttcattcttt tttaataatt tttttaaaac tgatacttta ttaatatcag
    13921 aagttataat aattttttca aaaaattgaa caatgccggt gttttcaatt tttgctcgct
    13981 gaaatttttt atcaccgtaa gagaccaaaa aaagatcaat gtttttttct gtttttttaa
    14041 aaaaattttc tacatctgga tataaaaaag tatttgctct atgtaaaaca tttttaaaat
    14101 tttctcttaa taattttaaa gaaacctctg gattatgttc aattattaat tgaaattgtt
    14161 tctcgggatt atagggtttt tctttagctg ctttttcaaa tgtctctttt aacaattttt
    14221 tattaatgcc aattttttga aaattgtttt caagggtatt gtaaaatctt tttaccgaaa
    14281 ataatgtatg gtcaaaatca aaaataagtt tcatattttt ttaattttaa aatttaggta
    14341 gattcaagtt tttaacgcaa tcatacaacg agatcgccac gttcggtctt cgttgtcatt
    14401 gcgaggagcc gcagcgacga agcaatcccg tggaatgttt gtgaacgtgc aacgggatcg
    14461 ccacgcccgc tttcagcggg ctcgcgatga cagaaagttt aaaaattaaa aattagaaat
    14521 ttaatagaaa ttaaaaatta aaaattaaaa attaataaag agtgttgcat taaattcttg
    14581 aatctaagct aatttcatta gttaaaaaag aaaggtgcgg tctttgagat tctttttaac
    14641 aactaagctt ggtccaacat tacagtttac accaataaaa actccgggca ttaaagagac
    14701 atttattcca atatttgaat tttcacctat caccacccct agtcttttaa gttgagttga
    14761 aattttttct cctttgacaa gagtgcttat ttcttttcta tctaatcttt tattggcggt
    14821 aacagtgcca gcaccaattt tacaattttc tccaaaaatt gaatcgccaa aaaatccgga
    14881 atgagtatga gaaccttctt caaaaataga tcttgctact tcagccaaag cgccaattaa
    14941 aacattattt tccaagttta catattctct tacaatagaa ttattgccaa taacacaatt
    15001 ttctccaata tagcagggac cctttattac agctccttca agaattttta cattattttc
    15061 aatttgaacc tttccctcaa taaccacatt tttcccaatt tttgcattct cagcaataat
    15121 tgattttaaa aatttatcca tcaaatattt ttccattaaa aataaatgcc agggatattt
    15181 taaagtaggc gtctcttctt cgggctcgtt taggattact attcgtgtat cattttttat
    15241 catatacaaa gaaagggttt cttcaaaatt atacattccc ttttttgctt ttttataaaa
    15301 ttcaaagaaa cctggctcta aaagataaac cccaactatc tttatgtctg acggttcttt
    15361 tcctggctgg ggtttttcaa caatttctaa aaccctattg ccttttagtt ttgccattcc
    15421 ataaagttgg gggtttcttg ttttttggcc aaataaaatg cctttaattt tagaattaaa
    15481 atttggaaat cttaaattta aaatttcatt aacatctact ctttcagcat ttaaaactaa
    15541 aaatttatct tttaacaaat cttttgttcg ccaaagcgca tcgcccattc caataggttt
    15601 tggctgaatc acatatctta tttttaaatt ttgaaatcga taattcttta attccttttc
    15661 aatatctttt gcttttccct caacaataat tacttcttta atgcctgatt tttttaatcc
    15721 attgatagta taccaaatta acggtttccc cattatttta aaaagggatt tatgtttttg
    15781 attaaacggc caaaaacgag atgactcacc ggctgctaaa attactgctt gtgttggtaa
    15841 aaatttttga agcatatttt atcaaaaatt aataatcttt tttaaaaaag aaaaaagttt
    15901 ttgctggtca tattttattt ctaatccatc caacaacaga tcttctccaa taaacttatt
    15961 tttgtccaaa tcattgttta ttatcggcgg gtttaaaacg aattcttctt ttttctcttc
    16021 ttggtatttt ttaattgtgt cttttgatgg aatattatta ttaaaaatta caaaatccaa
    16081 aggaacgccc aaatattttt caattgcttc tgtaaaatcc tttatggaat aattttgagt
    16141 ttctccccat tttgtcattg ctgggcaaat aaagattttt ttagcatgag attgtgccat
    16201 tgcctctttc catcctttgc ccaaaaaaca aggaattaaa ctagaataca aatcacctgg
    16261 cccaataatg atattatctg ctgttattat ctcgtctatg gctttttgat aagcccttgc
    16321 tttgggtttt aaataaacat tttttatttt caaatcgcgg ttatgatttt tcccaagatc
    16381 aatttcttcc tcgccttcaa tcaaactgcc atcttccaat tcagctacca attgaacctt
    16441 atctaaaata gcaggcaatg ccattcccct tacatttaaa aattgatgac aaatgtttaa
    16501 tgcttcttca aaattgccaa atatactttc aagtcctgta ataaaaacgt tggcaaaatt
    16561 gtgaccaaag tgttgaggag aaatttctat atctttggca aaacgaaact gccacaattc
    16621 ttttttccat ttttctccct cagacaaggc taataagtgc ctcctgatat ctccagcagg
    16681 cattgtattg aattcttttc taagagcgcc ggttgagccg ccattgtcca ccattgaggt
    16741 tatagaaaca atatcaaaat taaagttttt aagaggctct aaaattaatt gaggcattgc
    16801 tccgccacca ccaaaacaaa caattttttt ctttctcata tattaaattt ataacagatt
    16861 ttttaaaaaa ataaagggca cttggattta attttttaat attaaaacta gcccttgtaa
    16921 tttttacagg gctagtcaaa tttcatatat acctattagt aacagccga
//
LOCUS       DAOTQD010000009        14935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_3929_length_14935_cov_8.013239, whole genome shotgun sequence.
ACCESSION   DAOTQD010000009 DAOTQD010000000
VERSION     DAOTQD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3929_length_14935_cov_8.013239"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1358
                     /locus_tag="P5570_00790"
     CDS             63..1358
                     /locus_tag="P5570_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR39284.1"
                     /translation="MSNFFSIFKKEIKETINVQLIFSLVFTVLLFIWLGQMIGSQIEE
                     ATKTMKETSIALLDLDQTPTSNKIKSILSETDGGISFGIDEITETKEKTEAVKIAKEK
                     NLTNLLLIPSGFEKKLQSGEKAEIEVYSIFKGFSLGATTSSGQIESIVQALNNSLRDT
                     YFQEILPDKNIDNILNPIEIKNSVVVNDKIQEGDPAMVANLISSQNIFIPMILMLVIV
                     YCGTIMITSMATEKENKTLETLLTMPIKRETIIIGKMAGAAVVGLLMAVVYMFGFNYY
                     FNSMTVPQEEINKSLSLADFGLVMGPLEYIILGISIFCAILVALALSMLLGVFSESTK
                     SANTMIMPIVILAMIPFFLLMFQDIGEMSSAVRIILYIIPFSHPILASRALLFGEYQT
                     AILGILYLLVVLGIIISILLKIFNTDKILTAKFSFKKKK"
     gene            1471..1544
                     /locus_tag="P5570_00795"
     tRNA            1471..1544
                     /locus_tag="P5570_00795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1503..1505,aa:Gln,seq:ttg)
     gene            complement(2001..2738)
                     /locus_tag="P5570_00800"
     CDS             complement(2001..2738)
                     /locus_tag="P5570_00800"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HRR39285.1"
                     /translation="MRKVQPKEKVVLVGVLKQKRDLEIILKERWYRIPIDYAPKRQFD
                     YLAFYQPLSFGKEGKQIKYFAEVFDFKKVKRKDLLPSEINHPRVEKDYFQIRLKNIQK
                     LSQPIKNIHPRRISFGFTTLSRFLTARDVLQLYNVPPLENIVEKGLKNVGIKVLSQYR
                     LTINKKRFRLDFAIFCRKGKIAIECDNRKAHSNSLQKEKDKIKDEYLKNDGWEIIRLK
                     ENAVLSKLDSCLLKIQKTIQKLGGIAM"
     gene            complement(2805..3437)
                     /locus_tag="P5570_00805"
     CDS             complement(2805..3437)
                     /locus_tag="P5570_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HRR39286.1"
                     /translation="MNNIVMIKNSVKYWYLVLVAGIISIGVGLWVMIFPRESITVTAL
                     VFVLTFLITGLFETIFAIANRNAMTNWGWGLALGILNLIIGVLLLLNPAISVLTLAFY
                     VGFVILFHSLAAIIIAFDLKRYLILQWGNLLAMGIIGFILAVLLLVNPSLTTMVVIIF
                     LGLALIIGGTMSIYFSLKLKKIKERAQKISSDLMSRYEAIRKEIEDIFRG"
     gene            3856..4581
                     /locus_tag="P5570_00810"
     CDS             3856..4581
                     /locus_tag="P5570_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39287.1"
                     /translation="MPNQNKFSKKKIFLLISIVIIIILFFVGFLLGGLNKTNQKIVLE
                     RLNEAVNMVDNAPEIPSGLLEVHNYVLEELKKGQIPVGDITEIVPEAKTHPEDYPAQY
                     EMVNDILYINPYLNIKSEIWLPILYHELTHKWERNTQYKNTPKEHWQNSILELESTAY
                     ATTAQSWSLVQRYYPQDKDKLTPEEKTLVQSFETISNYYNDLQTTIEKENLDLQTQAG
                     WDKLQNTDAWRNWVNLIQSQVSK"
     gene            4646..4918
                     /locus_tag="P5570_00815"
     CDS             4646..4918
                     /locus_tag="P5570_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HRR39288.1"
                     /translation="MEDEKVEKIIEEYDKYARQNGFSLNSNKKVVEGIVKGLLKNEEK
                     YGARYCPCRRVSGYLEKDKAKICPCSWHRKEIVKYGHCLCCLFVKN"
     gene            4936..6270
                     /locus_tag="P5570_00820"
     CDS             4936..6270
                     /locus_tag="P5570_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRR39289.1"
                     /translation="MSNILTQLINICLLLLVVLLPASSLSLYFNIHPAVIIIFLLFLL
                     VIIRDHKAIFSGAFWKENKKIAPLIFLFSLLFLQSLLSLIFAPQMPPSAYDFGVGGFE
                     SPNLIRYYGIFTLLKSLLLGGLMAVIIKKDDFLKIINLHILTSVIFSLFGIINFLTYC
                     VLQVSSFDFGIMGGRVKSISVEPQAFASYLLTVIPFLIFGLCAKKTMFFKKGLLWTFL
                     IIDLFAFILTFSTGGLIAFLAIIVCFYLLLTVKNFRQYLSFKNIKIFTVFLLLLFVFA
                     LTIGKEEMTLMLQKFGTKESPSFSGRWDSWQAGIEMIKSRPLFGVGPESYGYFFKEYS
                     DAPVPQPTIGGPGAPAVILPLPQNLLIGTAANIGILGAVFFLLIFLYPVIKLWQQIKS
                     KEHTNNSVPIIAVSLLLVLVGLLVQNLAFWTPYAFFLWFFIGLAGAFINSYS"
     gene            6279..7421
                     /locus_tag="P5570_00825"
     CDS             6279..7421
                     /locus_tag="P5570_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRR39290.1"
                     /translation="MRIAIDARVIERRMSGIARYLLGFLEYLSCSDNKNQYFLFCYEE
                     LPRFKKLGYKIIATGKNKFLPDKIYNLYWQEFILPSLLKEHKIDVFFNPNHYLPLRKI
                     SSRGAITIHDLAHKIDPSYKSFIYRFLYLEKILPKSIKKADLILTVSESSKKDILKYY
                     GNLILKDNIKVIYPSTDERFGSNNYLQNYELVTNIKKKYCLPEEFILYVGRIEKRKNI
                     EGIFKIAALLPEKRFVLVGEGGYSGSKELLKKIKEQKNINHLKFVDDEDLPHIYNLAK
                     IFLFPSFYEGFGLPVLEAMKSGLPVLTSNTSSLPEVVGEGGIMHGPNNYQEFVKDIRK
                     LFEDEKFYQEMKKRAIAQAGKFNWKKSTEELVEVFEKSFGKEKILC"
     gene            7444..8679
                     /gene="tyrS"
                     /locus_tag="P5570_00830"
     CDS             7444..8679
                     /gene="tyrS"
                     /locus_tag="P5570_00830"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRR39291.1"
                     /translation="MQINTDSKKINELLTRGVEQIIEKESLRKKLKSGRQLRIKFGID
                     PTAPDIHLGNAVILWKLRDFQDLGHKIILIIGDFTAQIGDPTGKLSTRKMLTQQEIEE
                     NMKNYKNQIGKILDVKKIKIVYNSDHLGKLTLADIYQIFHFFTANQILERDMFQEREK
                     KGKPIWLHEFLYPIFQAYDSAAIKADVEIGGSDQLFNMTMGRQLQPHFGQDPQDVMTM
                     KLLIGTDGEKKMSKSVGNYIGIQESPEEQYGKIMSIKDELILDYFELCTRISLKKIKE
                     LEKDLKAKNVNPRDLKSRLAKKIVEIYHGKTAAKKAEKEFNRVFKEKKVPSKMPVFIV
                     PQESYPVLKLLVELNLAQSKSEAKRLIEEGGVKIIKPTIDNKQLTIKLRDWKEEIKIQ
                     DEMILKVGKRKFAKIKKSS"
     gene            8745..9623
                     /locus_tag="P5570_00835"
     CDS             8745..9623
                     /locus_tag="P5570_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRR39292.1"
                     /translation="MKNIGLVVGETASLPQEIINKYQMAFVPYLVDWSEGNDLPGENI
                     FQKMREADKRGIKTPPKTSQPSPWTFKKIFEEELLKSENLLCITLSSKLSGGYNSAIQ
                     ARKVLTDSDQPRVHIVDCFNVSAGEGLFDLKAIDLINEGKDIEEVVKEIKKFIHNVHL
                     FGMVEDPKWLEAGGRMSHPLAVLVRQMAKIGMRPLIGIKEGVVTSVALKMQAKDVPTA
                     LFKELQKETNELREKGKKIKVAITHADNPQGAQELQDLIESNLNNVEIAFVSLIDSII
                     GVHVGPGTIICAWCEA"
     gene            9712..10044
                     /locus_tag="P5570_00840"
     CDS             9712..10044
                     /locus_tag="P5570_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39293.1"
                     /translation="MEILWTKHSLDKMKFYGLSKQRVKRVLRNPKRIEEGIAPGTIAL
                     MQPAGSKKRPSEIWLMYVLTPSSSKIIISCWRYPGVSPVNKEIPLPEGMQLEIEKELK
                     KLKVDKDR"
     gene            10087..11772
                     /locus_tag="P5570_00845"
     CDS             10087..11772
                     /locus_tag="P5570_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRR39294.1"
                     /translation="MSRYKVKTQFKEELEPEEILLDSEEKKKEEKGRLELPIKASVFS
                     TIFLLFLFLFLVLFVRVFFLTAVEGKEYAEQAEKNYLRIFYVAAPRGEIYSSDGVLLA
                     GNIEKEMTETDDQNNIFKKEYIRTYPDGIIFAHVLGYLNSVDLDDLEADSYYQLNDKI
                     GREGIESKYENFLRGEKGRIDKVVNAKGNLIEGSEKIVSQPVQGDKLVLNINAGLQKK
                     LYETIKKRVPDKNASAIALDPQTGKILALVSIPSYDNNSVPSTQGSFFNRTIAGKYPS
                     GSIIKPLIAAAGLEEDIITPQTKINCQGKILIPGTSVFKNDWKVHGITDLNKALAESC
                     NIYFFGVGGGYGDIQGLGMQKIKKYLDLFYIEDKLGIDLPGEKVGFVPTEEWFNKSRV
                     TEEGRFWSIDDVYNASIGQGFFEATPLHMAVALSAIANGGKIYQPQIVNEILSIDGIV
                     KETVEPKILNEKFISSENIKAVKEGMRQCVVSSTGSCRQLISLPVTSGGKTGTAETAG
                     DQEPHAWFVSFAPYENPEIFLLVMVENGGGGEKVSAPIAKEVLEWYFQNQSLP"
     gene            11784..12113
                     /locus_tag="P5570_00850"
     CDS             11784..12113
                     /locus_tag="P5570_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012007685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HRR39295.1"
                     /translation="MTFKEYQKLSKKTAIYPNKGCNFIYPTLGLAGEAGEVAEKIKKV
                     IRDDEGLINDLKKKEISKELGDVLWYVSQIATELNLSLQDIALGNLQKLRSRQKRNKL
                     SGDGDNR"
     gene            12125..12619
                     /locus_tag="P5570_00855"
     CDS             12125..12619
                     /locus_tag="P5570_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRR39296.1"
                     /translation="MVTEVIKNRRSVREYKANEIPEKYINEIIKAGQFAPSACNNRAV
                     EFIVIKNQKIKDEIFRIIGQKFIKEAPVLIIPATDTRKTNFPIQDLSVASENMFLEAT
                     ALGLGTVWKAVSGVWEEKIKELLGVPKEYKIINVIPVGRPKERTFPHSEADFDPKKIH
                     KEKW"
     gene            13147..13413
                     /locus_tag="P5570_00860"
     CDS             13147..13413
                     /locus_tag="P5570_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRR39297.1"
                     /translation="MKKQEFIEEVAKKTKLPKSEVAEVVNTGIEVVAKNISKGVVLTG
                     FGTFSVSKRKARNGRNPKTGETIKIPATKVPRFKAGKALKQAVK"
     gene            13646..14773
                     /gene="mnmA"
                     /locus_tag="P5570_00865"
     CDS             13646..14773
                     /gene="mnmA"
                     /locus_tag="P5570_00865"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRR39298.1"
                     /translation="MKIAILMSGGIDSSFASYLLLKQGFNVIGITFLQTGNSPKKDFL
                     RAQKIAKTLSIPHFYFNIKNDFKKEVIVPFLEDFKNGKTPNPCPICNKKIKLGLMMKK
                     AKNLGADYIATGHYARKKQLATSDKQLVTYQLLKGKDKNKDQSYFLWMLSQRDLKKLI
                     LPLGGFTKEEVWQGIENSPLKKFFQKNEYYKESQDVCFLSSSSAYTKDHSKAKLSDFL
                     KRNLKTKPGPILDKDGKIIGDHQGIHFFTIGQRSGLHIGAKSPKQNPLYVVKIDKEKN
                     AVIVGEEKDLYKKEILIKDLHLISEKEIQLPLRVKVKIRARHKAAAATILPLEKKFTG
                     QSKNLKSNTYLIFDKPQRALAPGQHAVFYQRNILLGGGIIM"
BASE COUNT         5230 a         2187 c         2708 g         4810 t
ORIGIN      
        1 tttctgacaa ctgatttctg tttagtattt agaatttgtg attttgagtt ttatttaatt
       61 ctatgtctaa ttttttttca attttcaaaa aagaaataaa agagacaatt aatgtccagt
      121 tgattttttc tttggttttt actgtattgt tatttatttg gctagggcaa atgattggaa
      181 gtcaaataga agaagcaaca aaaacaatga aagaaacatc tattgctttg cttgatttag
      241 atcaaacgcc aacctcaaat aaaataaaat caattttatc tgaaacagat ggaggcattt
      301 cttttggaat agatgagatt accgaaacca aagaaaaaac agaagcagtt aaaatagcaa
      361 aggaaaaaaa tttaacaaat ttgcttttaa ttcctagcgg gtttgagaaa aaacttcaaa
      421 gcggcgaaaa agccgagatc gaagtttatt ctatttttaa aggattcagt ttaggggcaa
      481 caacatcatc tggtcaaata gaaagcattg tgcaggcctt gaataattcg ttgagagata
      541 catattttca agaaattctt cctgataaaa atattgataa tattttaaat cctattgaga
      601 ttaaaaattc agtagtggtt aatgataaga ttcaagaagg agatccagca atggtagcta
      661 atttgatttc ttcacaaaac atctttattc caatgatctt aatgcttgtt attgtttatt
      721 gcggaacaat aatgatcact tcaatggcaa cagaaaaaga aaataaaacc cttgaaactt
      781 tacttacaat gcccataaaa agagaaacca ttattatagg aaaaatggca ggagcagctg
      841 tggttggtct tttgatggca gtggtttata tgtttggatt taattattat tttaattcaa
      901 tgaccgttcc tcaagaggaa attaataaaa gtttaagttt ggcagatttt ggccttgtaa
      961 tgggtccttt agaatatata attttaggaa tatcaatttt ttgtgctatt ttggttgcgc
     1021 ttgccttatc aatgcttctt ggagtctttt ccgaaagcac aaaaagcgcc aatacaatga
     1081 taatgcctat tgtaattttg gcaatgattc cattttttct tttgatgttc caagacattg
     1141 gcgaaatgtc cagtgccgta agaatcattt tgtatattat tcctttttct catccgattt
     1201 tggcttcaag ggctttactt tttggcgagt accaaactgc tattttggga attttgtatc
     1261 ttttggtggt tttgggaatc attatctcaa ttcttttaaa aatttttaac actgataaga
     1321 ttttgactgc taaattttcc tttaaaaaga aaaaataaaa ttagggactg atttaaagtt
     1381 ttaacaatgg aacgagaaac gatctcgttc tattgtttta atttgaaatt ttggggagat
     1441 aatgatatta taattttgca aaaacaaaaa tgcggggtcg tctaatggta ggacagtggc
     1501 ctttggagcc attaatctag gttcgaatcc tagccccgca gccactgtgc aaaaacagag
     1561 gtcttgacct ctgtttttgc tttttaaaag ggttttttac taattctaat tttattagat
     1621 attctatatt tacttatctt ataaagatgt ttattttttt ctcaaaatat gctattaaaa
     1681 tattgtctgc ccatttttta tatataagtg taaaacaact gttcattttg agaaatcccg
     1741 attgcgggtc aagatgggca gtcaacctcc acaatcggga ttttacactt accgtaaata
     1801 gtgggcagac aggttaactg cccattttaa ttttgaggaa aaaataaaaa aggccttaat
     1861 aaaaattcat aagcaagaaa agataaggta atagtttttt ccgttccggg tggtcctcag
     1921 taaaattttt ctgcttcgac ttggtccctt ttaaattctt gaacctaagt aaattaaatt
     1981 gtgattattt tttaaaaatt ttacattgct ataccaccta gtttttggat tgtcttttgg
     2041 atttttaaaa gacaactatc taattttgat aaaactgcat tttcttttaa acgaataatt
     2101 tcccaaccat cattttttaa atattcatct tttattttat ccttttcttt ttgaagggaa
     2161 tttgaatggg cttttctatt atcgcactca atcgcaatct ttccttttct gcaaaaaatc
     2221 gcaaaatcaa ggcgaaacct ctttttattt atcgtaagac gatattgact taaaacttta
     2281 atgccaacat tttttaaacc tttttcaaca atattctcaa gtggaggaac attatacagt
     2341 tgtaaaacat ctcttgctgt taaaaatcga cttaaggtgg taaatccaaa agagatccgc
     2401 cggggatgaa tattttttat tggctgggat agtttttgaa tgtttttaag acgaatttga
     2461 aaataatctt tttcaacgcg tggatgattt atttcacttg gtaataaatc ttttctttta
     2521 acttttttaa aatcaaaaac ttcagcaaaa tatttaatct gttttccttc tttgccaaaa
     2581 gaaaggggct gataaaaagc caaataatca aattggcgtt ttggcgcata atcaatagga
     2641 atgcgatacc atctctcttt taaaataatc tcgagatctc ttttttgttt taatactccc
     2701 accaaaacaa ctttttcttt tggttgaact tttctcatat tattttttaa aaaaaacggc
     2761 gagcaaaaaa atatggctcg ccgctgataa aatataaaaa gtttctatcc tctaaaaata
     2821 tcttctatct cttttctaat cgcttcgtaa cgggacatta aatcagagga aattttttgc
     2881 gcacgttctt tgatcttttt aagtttcaat gaaaagtaga tactcattgt accaccaatt
     2941 attaaggcaa gccccaaaaa gattattaca accatcgtag tcaaagacgg attaacaagt
     3001 aaaagaacgg ctaggataaa tcctattatt cccattgcca gtaaattacc ccactgtaaa
     3061 atcaagtatc tcttaagatc aaaagcgata ataatagcag ccaaggagtg aaaaagaatt
     3121 acaaatccaa cataaaaagc cagggtaaga actgaaatcg ccggattcaa cagtaaaaga
     3181 acaccaatta tcaagtttaa aatacccaag gcaagtcccc aaccccaatt agtcattgca
     3241 tttctattgg caatcgcaaa tattgtttca aaaagaccag tgattaaaaa tgtcaaaaca
     3301 aaaacaagag cagttaccgt gattgactcg cgaggaaaaa tcattaccca caacccgacg
     3361 ccgattgaaa tgatgcctgc aacaagtact aagtaccaat atttcactga attttttatc
     3421 attactatat tattcatacg ctcaaaatat taaattttta tttacttttc gacctttaat
     3481 ttataataac acacttttgt ttaaaataaa atctttactt cttgtattct ttttgccacc
     3541 tctactgttt tccgatttct gacaactgat ttctgtttag tatttggtat tttattattt
     3601 gtaaatgttg cattaaatta ttaaacccaa cttatcttat aaagatgttt atttttttct
     3661 caaaatatgc tattaaaata ttgtctgccc attttttata tataagtgta aaacaactgt
     3721 tcattttgag aaatcccgat tgcgggtcaa gatgggcagt caacctccac aatcgggaat
     3781 ttacacttac cgtaaatagt gggcagacag gttagctgcc cattttaatt ttaataaagg
     3841 gcataatgtc tgcttatgcc taatcaaaat aaattttcca aaaagaaaat ctttcttctt
     3901 attagtattg taattattat tatccttttc tttgtaggtt ttcttttagg tggattaaat
     3961 aaaactaatc aaaagattgt acttgagaga ttgaatgaag cagtaaatat ggtagataat
     4021 gctccagaga tcccctcagg gttattggaa gttcataact atgtgttaga agaacttaaa
     4081 aaaggacaaa ttcctgtagg agatatcaca gagatagttc cggaagcaaa aacccacccc
     4141 gaagactatc ctgcccaata tgagatggta aatgatattt tatatatcaa cccatacttg
     4201 aatattaaaa gtgaaatctg gctacctatc ttatatcatg aacttactca taaatgggaa
     4261 cgcaacactc aatacaaaaa caccccaaaa gaacactggc aaaacagtat tttagaactt
     4321 gagagtactg cctatgctac cactgctcag tcctggtcat tagttcaaag atactatccg
     4381 caagacaaag ataaattaac cccagaagag aaaacccttg ttcaaagttt tgaaacaatc
     4441 tctaactatt acaacgatct ccaaaccaca atagaaaaag agaatctcga cttgcaaact
     4501 caagcaggat gggataaact acaaaataca gatgcctggc gcaactgggt taatctaatt
     4561 caaagccagg tgagtaaata aaggttgaaa tgttgcgtta atttcttgaa cttaagcaag
     4621 aataagattt gataatattt ttactatgga agatgaaaaa gttgaaaaaa ttattgaaga
     4681 atatgataaa tatgcccggc aaaacgggtt tagtttaaat tccaataaaa aagtggtaga
     4741 aggcattgtg aagggattgc ttaaaaatga agaaaaatac ggtgcccgtt attgtccctg
     4801 cagaagagtg agtggctatt tagaaaaaga taaagcaaag atttgccctt gcagttggca
     4861 tcgtaaagaa attgtaaaat acggacactg tctttgttgt ttgtttgtaa agaattaaca
     4921 ttagatattt ttcttgtgtc aaatattctt actcaactaa taaatatttg tttactctta
     4981 ttggtggtgt tacttccggc aagttctttg tctctttatt ttaatattca tccggcagtg
     5041 attattattt ttttgctttt tttattggtg atcatccgcg atcataaagc gattttcagt
     5101 ggagcgtttt ggaaagaaaa caaaaaaatt gcgcctctaa tttttttgtt ttctttactc
     5161 tttttacaat ctctcctctc gttgatattt gcgcctcaga tgcctccttc tgcctatgat
     5221 tttggcgttg gcggatttga atcaccaaac cttattcgtt attatggcat ttttactctt
     5281 ttaaaatcat tgcttttggg ggggttaatg gcggtaatta taaaaaaaga tgatttttta
     5341 aagattatca atcttcatat tttaacctca gtaatttttt ccttatttgg aattattaat
     5401 tttttgacat attgtgttct tcaggtatcg tcatttgatt ttggtataat gggtggaaga
     5461 gtaaaatcaa ttagtgttga gccccaggct tttgcgagtt atcttttaac agtgattccg
     5521 tttttgattt ttggattgtg cgcaaagaaa acaatgtttt ttaaaaaagg gttattgtgg
     5581 acatttttaa ttattgatct ttttgctttt attttgacat tttcaaccgg cggtttgatt
     5641 gcgtttttgg caataattgt ttgtttttat cttttattaa ctgtaaaaaa ctttcgtcag
     5701 tatctatctt ttaaaaatat aaaaattttt acggtttttc ttttgctgtt gtttgtattt
     5761 gctttaacca taggaaaaga agagatgacc ctaatgttgc aaaagtttgg aaccaaagaa
     5821 tcgccttctt tttctggcag atgggatagt tggcaggccg gcattgaaat gataaagtcc
     5881 agacctcttt ttggagtggg accagaaagt tatggatatt ttttcaaaga atatagtgat
     5941 gctcccgtgc cccaacccac cattggcgga cctggtgctc cagctgttat tttgcctctg
     6001 ccgcaaaatt tgttaattgg tacagctgcc aatattggta ttttaggagc tgtgtttttt
     6061 cttttgattt ttttataccc tgttataaaa ttatggcagc agattaaaag taaagaacat
     6121 acaaacaata gtgttcctat aatagctgtt tccttgttgc tggtgctggt ggggttgctt
     6181 gttcaaaatc tagctttttg gacgccctat gctttcttct tgtggttttt tataggctta
     6241 gcaggggcat ttattaattc gtattcttaa ttattattat gcgtattgct attgatgcaa
     6301 gagttattga aagaagaatg agcggtatcg caaggtatct tcttggtttt ttagaatatc
     6361 tttcttgttc tgataacaaa aatcagtatt ttctattttg ttatgaggaa cttccgcggt
     6421 ttaaaaaatt gggatataaa attattgcca caggaaaaaa taaatttttg cctgataaaa
     6481 tttataatct ttattggcag gaatttattt tgccgtcgct tttaaaagaa cacaaaattg
     6541 atgttttttt caaccccaat cattatttgc ctttaagaaa aatttcatca agaggagcga
     6601 ttactattca cgacttggcg cataagattg atccatcgta taaaagtttt atttatcggt
     6661 ttttatattt agaaaaaatt ttaccaaaat caataaaaaa agcagatttg attttaacag
     6721 tttcagaaag ttctaaaaag gatattttaa aatattacgg aaatttaatc ttgaaagaca
     6781 acattaaagt gatttatcca tcgaccgatg aaaggtttgg atctaataat tacttacaaa
     6841 attacgaatt agttacgaat ataaaaaaga aatattgttt gccagaggaa tttattttgt
     6901 atgtgggaag aattgaaaaa agaaaaaaca ttgaagggat ctttaaaatt gccgctttgt
     6961 tgcctgaaaa aagatttgtt ttggtgggcg agggtggtta tagtggttcc aaggaattat
     7021 taaaaaaaat aaaagagcaa aagaatatta atcatttgaa atttgttgat gatgaagatt
     7081 taccccatat ttataacctc gcaaagatat ttctatttcc ttctttttat gagggttttg
     7141 ggttgccggt tttagaagca atgaaatctg gtttgcctgt tttgacctca aacacctctt
     7201 ctttgccgga agtagttggc gagggtggga taatgcatgg tcctaataat tatcaagaat
     7261 ttgttaaaga tattaggaag ctgtttgaag atgaaaaatt ttatcaagag atgaaaaaac
     7321 gagcaattgc tcaggctgga aaatttaatt ggaaaaaatc aacagaagaa ttagtagaag
     7381 tattcgagaa gtcatttggc aaggagaaga ttctatgtta aaatattttc taaactaaaa
     7441 aatatgcaaa ttaatactga ttccaaaaaa ataaatgaac ttttgacccg aggagtggaa
     7501 cagattattg agaaagaaag cttaagaaaa aaattaaaat ccggacgtca attacggata
     7561 aagtttggta ttgatcccac tgctcctgat attcatttgg gcaatgcagt aattttgtgg
     7621 aaattaagag attttcaaga tttaggacat aagattattt taattattgg tgattttact
     7681 gctcaaattg gtgatcctac aggaaaatta agtacaagaa aaatgcttac tcagcaagag
     7741 atcgaagaaa atatgaaaaa ctataaaaac cagataggca aaattttaga tgttaagaaa
     7801 attaaaatag tttacaatag cgatcattta ggaaaattaa cccttgcaga tatttatcag
     7861 atttttcatt ttttcacagc taatcaaata ttagaaagag acatgtttca agaaagagaa
     7921 aaaaagggaa aacccatttg gcttcatgag tttttatatc ctatatttca agcctatgat
     7981 tcggcagcta ttaaggcaga tgttgagatt ggtggtagtg atcaattgtt taatatgaca
     8041 atgggaagac agcttcaacc acattttggg caagaccctc aggatgttat gactatgaaa
     8101 ctattaattg gaactgacgg tgagaagaaa atgagtaaaa gcgttggaaa ttatattggc
     8161 attcaagaat caccagagga gcaatatgga aaaattatgt ctattaaaga tgaattgata
     8221 ttagattatt ttgaactttg caccagaatc tctttaaaaa agataaaaga gctggaaaaa
     8281 gatttaaaag caaaaaatgt caatcctcga gaccttaaaa gtcgtttggc aaaaaaaatt
     8341 gtagagatat atcacggaaa aacagcagct aaaaaagctg aaaaagaatt taatcgagtg
     8401 tttaaagaaa aaaaagttcc ttcaaaaatg cctgttttta ttgttcctca agaatcatac
     8461 ccagttttaa aacttttggt tgaattaaat ctagctcaat caaaaagcga agccaaaaga
     8521 ttaattgaag agggaggtgt taaaattata aaaccaacaa ttgataataa acaattaaca
     8581 ataaaattaa gggattggaa agaagaaatt aaaattcaag atgaaatgat tttgaaagtg
     8641 gggaaaagaa aatttgcaaa gattaaaaag tcaagttaat aatttaataa cgtgctgtgt
     8701 tttaaattat cttatagaaa aatttctaac acggtaaaaa aaatatgaag aacattggtt
     8761 tggtagtagg ggaaactgca agtttgcctc aagaaataat aaataaatat cagatggcat
     8821 ttgtgccata tttggttgat tggtctgaag gcaatgattt gccaggcgag aatatttttc
     8881 aaaaaatgag agaggccgat aaacgtggca ttaaaacccc tcccaaaacc tctcaacctt
     8941 ctccctggac ttttaaaaaa atttttgaag aggaattatt aaaaagtgaa aatcttcttt
     9001 gtatcaccct atcttcaaaa ttatccggcg gttataattc agcgatccag gcaagaaagg
     9061 tgcttactga ttctgatcag ccaagagtac acatagttga ttgttttaat gtcagtgctg
     9121 gcgaaggact gtttgattta aaagcaattg atttaatcaa tgaaggaaaa gatattgaag
     9181 aggtggtaaa agaaataaaa aaatttattc ataatgttca tttgtttggt atggtagaag
     9241 acccaaaatg gttagaggca ggaggcagga tgtcccatcc tttggcggtt ttagtaagac
     9301 aaatggcaaa gattggaatg aggccgttaa tagggatcaa agaaggcgtg gtaacctcag
     9361 ttgccctaaa aatgcaggca aaggacgtac ctacggcatt gtttaaggag cttcaaaaag
     9421 aaaccaatga gttgagggaa aaaggaaaaa agataaaagt agctattact cacgctgaca
     9481 atcctcaagg cgcccaagaa ttacaagatt tgattgaaag caatttaaac aatgttgaaa
     9541 ttgcttttgt aagcctgatt gattccatta ttggtgttca tgtggggcct gggacgatta
     9601 tctgcgcttg gtgcgaggca tagtgtttga atttactttg cagattctat ccgaaaatgc
     9661 gcattcaata atattctaat cagtcggata aattcacaga tattgtgttc tatggaaatt
     9721 ttatggacaa aacattccct tgacaagatg aaattttacg gtttatcaaa gcagcgcgta
     9781 aaaagagttt taagaaatcc aaagagaatt gaagaaggga ttgcaccagg aactattgcc
     9841 ttaatgcaac cagccggttc aaaaaaacga ccatcagaaa tttggttgat gtatgtgctc
     9901 acacccagct ctagtaagat tattatttct tgttggcgct atcccggtgt aagtccagtg
     9961 aacaaagaaa ttcctcttcc agaaggaatg cagttagaaa ttgaaaaaga attaaaaaag
    10021 ttaaaagttg ataaagacag ataatctctt ttttccattt aactccttct atttatttat
    10081 taaaaaatgt ctcgttacaa ggttaaaact caatttaaag aagaacttga accagaagaa
    10141 attctgcttg attcagaaga aaaaaagaaa gaagaaaaag gacgacttga acttcctatc
    10201 aaggcatctg tcttctctac gatttttctt ttgtttttat ttttatttct ggtgcttttt
    10261 gtaagagtat tttttttaac agcagtagaa ggaaaagaat atgctgagca ggcagaaaaa
    10321 aattatttaa ggatttttta tgtggctgct ccacgaggtg aaatttattc aagcgatgga
    10381 gtattgctgg ctggaaatat tgaaaaggaa atgacagaaa ctgacgacca gaacaatatt
    10441 tttaaaaaag aatatattcg cacttatcct gatgggatta tttttgctca tgtattgggt
    10501 tatttgaatt ctgttgactt ggatgatctt gaagcagatt cttattatca acttaatgat
    10561 aaaattggaa gagaaggcat agaaagtaag tacgagaatt ttttgcgtgg agaaaaagga
    10621 cgaatagata aagtagtcaa cgccaaggga aatcttattg aaggatcaga aaaaattgtt
    10681 tcacagccgg tgcagggaga taaacttgtt ttgaatatta atgctgggct tcaaaagaaa
    10741 ctttatgaga ccataaaaaa aagagtgcct gataaaaacg ccagtgccat tgccctggat
    10801 ccccaaaccg gcaagatttt agctctggtt tctatcccta gttacgataa taattctgtt
    10861 ccctctacac aaggttcatt ttttaatcgg actattgctg ggaaataccc ttctggttca
    10921 atcattaaac ctttaattgc tgcggctgga cttgaagagg acattattac tcctcaaaca
    10981 aaaataaatt gtcaaggaaa aattttaatt cctggcacgt ctgtttttaa aaatgattgg
    11041 aaagttcatg gtattactga tttaaataag gcactagcag aatcctgtaa tatttatttt
    11101 tttggtgttg gaggaggata tggagatatt cagggattag gaatgcaaaa aattaaaaaa
    11161 tacttagatc tgttttatat tgaagacaag ctgggaattg atttgcctgg agaaaaagta
    11221 gggtttgtgc caacagaaga gtggtttaat aagagccgcg taactgaaga agggcgtttc
    11281 tggtcaatag atgatgttta taatgcttcg atcggtcagg gattttttga agctactcct
    11341 ttacatatgg ctgttgctct ttctgctatt gccaatggtg gaaaaatcta tcaacctcag
    11401 attgttaatg agattttaag cattgacggc attgtaaaag aaaccgtaga gccaaaaatt
    11461 ttaaatgaga aatttatttc ttcagaaaat attaaggcag tgaaagaagg aatgagacaa
    11521 tgtgttgttt cttccacggg ctcttgccgt cagctaatat ccttgccagt aacctcaggc
    11581 ggaaaaaccg gtactgctga aacagcagga gatcaagaac cccatgcttg gtttgtttct
    11641 tttgctccct atgagaaccc tgaaatattt cttttggtga tggtagaaaa tggtggcggt
    11701 ggtgaaaaag tgtctgcccc cattgctaaa gaagtattag agtggtattt tcaaaatcaa
    11761 tcattgccat aaaaataaaa aatatgacat ttaaagaata tcaaaaactt tcaaaaaaaa
    11821 cagcaattta tcccaataag ggttgtaatt ttatttatcc tactttgggt ttggcgggcg
    11881 aagcaggtga ggtggcagaa aagataaaaa aggtgataag ggatgatgaa ggattaataa
    11941 atgatttgaa aaagaaagaa atttcaaaag agttaggaga tgttttatgg tatgtgtctc
    12001 aaatcgcaac agaactaaat ctttctttgc aagacattgc tttgggcaat cttcaaaaat
    12061 taagatcgcg tcagaaaaga aataaacttt caggcgatgg tgacaataga taaattttaa
    12121 atttatggtg acagaagtaa taaaaaatcg acgttcagta agggagtata aagccaatga
    12181 gattcctgaa aaatacatta atgaaattat taaggcagga caatttgctc ctagtgcgtg
    12241 caataatcgg gcagtggagt ttattgtgat taaaaatcaa aagataaaag atgaaatttt
    12301 tagaattatt ggtcaaaaat ttatcaaaga agcaccagta ttgattattc ctgcaactga
    12361 taccagaaaa accaattttc caattcagga tctgtcggtt gcttcagaaa atatgttttt
    12421 ggaagctaca gccttgggat tgggaacagt gtggaaagca gtaagcggtg tgtgggaaga
    12481 gaaaattaaa gagctgcttg gtgtgcccaa agaatacaaa attatcaatg taattccagt
    12541 aggccgtcca aaagaaagaa catttcctca tagcgaagca gattttgatc ccaaaaaaat
    12601 tcacaaagaa aagtggtaaa tgattttttc aaaataaaaa ataaatcctt aaagatttat
    12661 ttatattatt ttttaataat aaaagaatta tcgtcagcta aatatactgg tattctttat
    12721 gatagcagtg gcgtggagag cagatgagct atcggttggt aggtaaaata tttgtttcaa
    12781 ataattatcg gattgaggtt gtttcaaatg cgctgtatag ccaaaaataa taattcggag
    12841 cgctgggaat tgaacccagt ctgcaccctc ccgaagggtg tgtactgccg gtatacgacg
    12901 ctccgtataa agattaattt tattatatta aaaaagcttg caattatcaa ttttaaattt
    12961 tataataaat taatatacaa aacattgtat taatttgata atcttaaccc agtaagaagt
    13021 ttaataaata agataagttt ctaaaactgg gtgaagccct tgtgaggcat taccaaagat
    13081 taaatttgga agccatcata gggaaaggtc gatattttaa aaaaagattt gtaaaaatca
    13141 aaatatatga aaaagcaaga atttattgaa gaagttgcca aaaaaacaaa acttccaaag
    13201 agcgaagtgg cagaggtagt aaatactggc attgaggtgg ttgccaagaa tattagcaag
    13261 ggagtggtat taactggatt tggcaccttt agtgtgtcaa agagaaaagc cagaaatggt
    13321 agaaatccca aaacaggtga aacgattaaa attcctgcaa ccaaagtacc acggttcaaa
    13381 gcaggaaagg ccttaaagca ggcagttaaa taaggatttt ggaaagtaag gtttttttga
    13441 tataatcccc caaaggcata ctttcctttg gggggttatt ttaattagag agtatgaaaa
    13501 tagcgaggtc tatgaagttt gcttccttca ggatttcaca aaaatttctt ttttgcctcg
    13561 tccttctccc cctcaccttc cttaaagaag gtgaggggtt cggagtttac cttcatcttc
    13621 tcgctactct cattttcgtt tatatatgaa aatagcgata ttaatgagtg gaggaattga
    13681 ttcttctttt gcatcttacc tacttttaaa gcagggtttt aatgtaattg gaatcacctt
    13741 tttacaaacg ggcaatagcc ccaaaaaaga ttttttacga gctcaaaaaa tagcaaaaac
    13801 actttcaatt cctcattttt attttaatat caaaaatgat tttaaaaaag aggttattgt
    13861 tccgttcctt gaagatttta aaaatggtaa aacaccaaac ccttgcccaa tatgcaataa
    13921 aaaaattaaa ttaggtttga tgatgaaaaa ggcaaagaat ctaggagcag attatattgc
    13981 tactggtcac tatgccagaa aaaaacaact tgcaactagc gataaacaat tagtaactta
    14041 tcaattgctt aaaggaaaag acaaaaacaa ggaccaatcg tattttttat ggatgctttc
    14101 ccaaagagat cttaaaaaac ttattttacc cctgggcggt tttaccaaag aagaagtttg
    14161 gcaggggata gaaaattcac cactcaaaaa gttttttcaa aaaaatgaat attataaaga
    14221 aagtcaggat gtttgttttt tatccagttc cagcgcttat acaaaagatc attcaaaagc
    14281 aaaactttca gattttttga aaagaaattt gaaaacaaaa ccaggtccaa ttttagataa
    14341 agatggcaag ataatcggcg atcatcaagg aattcatttt tttactattg gccagcgaag
    14401 cggtcttcat attggcgcga aaagtccaaa acaaaatccc ttgtatgttg taaaaattga
    14461 taaggaaaaa aatgcagtga ttgtggggga ggaaaaagat ctatataaaa aagagatttt
    14521 aataaaggat ttgcatttga tttctgaaaa agaaatccaa ttgccattaa gggtaaaggt
    14581 taaaattaga gcaagacata aagcagcagc agcaactatt ttaccgttag aaaaaaaatt
    14641 tactggtcaa tccaaaaatc taaaatctaa tacctattta atatttgata agccccaacg
    14701 agctttagcg ccaggccaac acgcagtttt ttatcaaaga aatattcttt tgggaggagg
    14761 aataataatg taataaattc caatgtgaag gtgagaggga agtaatctca aggtaacatc
    14821 caaaagttac attggaaatt ccaaatcaca agtaccaaaa tacaaacaag caccaaatca
    14881 caaattccaa ttttcaatca aattccaagc ttagcgaagc caaacgaagg gcgaa
//
LOCUS       DAOTQD010000010        14633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_4022_length_14633_cov_7.723076, whole genome shotgun sequence.
ACCESSION   DAOTQD010000010 DAOTQD010000000
VERSION     DAOTQD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14633
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_14633_cov_7.723076"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1123)
                     /locus_tag="P5570_00870"
     CDS             complement(5..1123)
                     /locus_tag="P5570_00870"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRR39299.1"
                     /translation="MRILFTGGGSGGHIFPIIAVKREIDNLVPEEIKSSLFQFLGGKT
                     QETEVLLKENISFKKIVTVKWRRYFSFKNILDIFKFPISFLQALFKVWLIMPDLIFSK
                     GGPGSFPVMLVGWLYQIPIVIHESDSVPGSTSKLSSPIARKIAISFKESEDFFPKKHK
                     RKLVLTGNPIRQKLFYGSKEKAIKNFNLNGKRKIILIMGGSQGSEQINSLFLDALLKY
                     IEKYEIIHICGPNNFKNIDLFTRGALDDAQKVFYHLYPFLDEEQLADAYQASDLVISR
                     AGAGAIFELAALEKPSILIPLQGGAQDHQSKNAQCFQKYGATISVEGCNVTPNFVFGR
                     ANQILENKKTLQEMKNGCKKFAMPDAAKKVATVILEEI"
     gene            complement(1218..1805)
                     /locus_tag="P5570_00875"
     CDS             complement(1218..1805)
                     /locus_tag="P5570_00875"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HRR39300.1"
                     /translation="MFSKIIPIIIAYFLGSIPSAYLIAKNKGIDITSRVENGRLGTAS
                     VSRNVGKFAGALVGIMDFCKGALSIIIADRMSGGEGWVMVLAGAAAIIGHNWSIFLHF
                     MGGKGAATTFGNLFYLLPIPFLLAGFITAVPSYFLKQKSRFSIPGKNKDFKTSDFLTS
                     ILFLLTFIFALFFEAPLIIAFSPIIFSVPIILKKN"
     gene            complement(1798..2406)
                     /locus_tag="P5570_00880"
     CDS             complement(1798..2406)
                     /locus_tag="P5570_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR39301.1"
                     /translation="MSFQSSVVKFNNLTFGKIVQKGGLQSFFSKIYLPKNAYFLEIGC
                     KDGYALPIINQYFAPQKMIGVDIDTDSLKIAQNRILKEQIKNTEVKPADAQQLPFDSE
                     IFDAIFMFATLHHIPDWKKALNEVSRTLKSGGYFIFREPLAKFYNLPFSKYFDKPAAL
                     FDKEDLEEALKLNNFKITYFNWRGIYKRFFHSSVEVVCQKNV"
     gene            complement(2508..3203)
                     /locus_tag="P5570_00885"
     CDS             complement(2508..3203)
                     /locus_tag="P5570_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRR39302.1"
                     /translation="MEIGGLQKTTLIDYPGCIAATVFTIGCNFRCPFCYATELVLLEK
                     IKDQPRISEKDFFEFLTSKKGLLDGVCICGGEPTIHKDLPQFCQKIKKLGFLVKLDTN
                     GSNPDIVKKLIDEKLVDYIAMDIKSPKEKYEIYTNKKIDLSLIEKSINLLKENRIDYE
                     FRTTVAPGLEKEDIINIAQWIGPAKKYFLQEMNEQKAIIEPDILSLPVLKKEDLIQII
                     KLIKPKFEICQLR"
     gene            complement(3210..3770)
                     /gene="frr"
                     /locus_tag="P5570_00890"
     CDS             complement(3210..3770)
                     /gene="frr"
                     /locus_tag="P5570_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRR39303.1"
                     /translation="MDTNQLFKNTKDSFEKTLDWFKKEVLIIRGGRVSIELIDGILVE
                     YYGEKITLREVASLSLLDARTISIEPWDKSSIPNIEKALQASNLGGSVRNEGEKIFFS
                     LPMQSAEDKEKMIKLVRQKMEKAKVALRQTREESWKKIQEMERKGEIPEDEKFRQKND
                     LQELINDYEKKIEEIEEKKEKEIQGS"
     gene            complement(3796..4938)
                     /locus_tag="P5570_00895"
     CDS             complement(3796..4938)
                     /locus_tag="P5570_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39304.1"
                     /translation="MFQQLGLALISFFVAYLCFIGVFNFLENNYGVEVSMGEQLFLSE
                     DRGEEWVALKSIDKADKKASIENLVFDPKNSQNLYLASAKGVFESSTKGEKFTAKTTT
                     FKTESQPAVVSNLTTDPRNPDVFYLVSEEVGQNKLLVSYNKGADFHPLFIASNDDKIK
                     TFAIDPFYSRHFLFSNRLYLGTKQGSFLKSEDFGNSWKETYKFSQPIEKIVLNPHQSG
                     EIYILLSSIARDPFNVYSNGLPSKIEISVDSGETFKLIKEEIDTSKVENEIGELPEIK
                     DIITDPSQNRIYLISDYYLFRISRNEMDLVKIVSSSEKNKITAFTVDPKNSNILYVGM
                     GNLIYLSENDGENWQVIEPPTRGMIEKIKINPADPDTILLSVKKTF"
     gene            complement(4982..6001)
                     /locus_tag="P5570_00900"
     CDS             complement(4982..6001)
                     /locus_tag="P5570_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39305.1"
                     /translation="MIFGHQKNLKIFSKMIAENRFPHAVLFAGPEKIGKRKIALEIAK
                     YLETDHSSSFDNFFEFSQGNCSCDICKQIEEKNFYDLITIDKEKKEKKSSVKDNNFVK
                     DRQIHIKKIRDIKQQISLSSPYPFKIVIIDHSESLTEEATGSLLKILEEPRGNTIFFL
                     LTSSPNYLPTTILSRVEMFKFYPLSRIEMEAFLKSQPDQKEKEEIIGLVLGRPGLAKE
                     FLIDKNKIIYYNLLLGEIKKIRNLSILQRLKLAEDLEKTERTQDFLFLTEFWFRDLLL
                     RKQGLSSFYPSFFSKEKELEEEKENFSEIKLKEIIFKIKETKNYLYFSNTNPLLAMEN
                     LLLGI"
     gene            6565..7596
                     /locus_tag="P5570_00905"
     CDS             6565..7596
                     /locus_tag="P5570_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HRR39306.1"
                     /translation="MFNINDCKQTVDGILKEMPDRTQKVLSRRFGIDPENKSTNRCCE
                     TLESIGKDFGITRERVRQIENRGLEEFKRSSYFADVQRFFLFLRSFIDEQGGLKRQDI
                     LEKTLVPEEQLRPYLLFILKISNLFLFEPESSSFYSSWKTREEVSDIADRVVRFLIDL
                     MEKEKRLFSEEELLERGTKEVPKYLHLTIPTNHLISYIEATKKIRKDQFNNYGPVWWP
                     EVNPRGNRDYAYMILKIEKRPLHFREIAKKIEESFQRPVQAGSLHNELIKNDKFVLVG
                     RGTYGLKEWGYKNGTVKDIIEDILKENGPLTRNEVLNEVQKQRMVKRDTILLNLSSFK
                     KTPDGKYIL"
     gene            7640..8917
                     /locus_tag="P5570_00910"
     CDS             7640..8917
                     /locus_tag="P5570_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39307.1"
                     /translation="MSLYLEEFLNLLLSLWWLWLAILLFPLAKYFFVWAKKDHWFGSL
                     KYKMLEIKIPEEIEKTPKAMENVFHSMWGMYDPPSNIRDYWLDGKDMLYYSFEIVGRQ
                     DEVHFYIMAPNTHVQLVKSSIWAEYPEAEIKEVEDYISNFGKEVPNEDYDLWGTDLKL
                     AKPDAYPIRTYDYWETELTKDEKKIDPLAALFEAFGDLKEGEEVWVQLKVAPVTEDEH
                     PYLAESQKVINKIMKRPETKKKGILAPLGLSRIPSDVWTVLIEGKPIPPAMEEELPDG
                     FDIGLMKLSPGEAEALRAIEDNLGKYVYEGNVRFLYIAKYDIISKPRGITTVMSAFNQ
                     FATVNLNSLKPDRSKTSVIPWFFEGRRLYVKKRRIFRYYTGRAWPWHRSPYIFSTAEL
                     ATMYHFPGKGVAPSTAVPRVEIKRGEIPPNLPT"
     gene            8934..10361
                     /locus_tag="P5570_00915"
     CDS             8934..10361
                     /locus_tag="P5570_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRR39308.1"
                     /translation="MEEKEKVVHFAKTVYHDREKEFGIKTDDRRRHIYIVGKTGMGKS
                     CLLENMAIQDIQNGQGVGIVDPHGELAEKLLDFVPKNRINDVIYFNPADLDYPIAFNV
                     MEKVEFTHRHLVSAGLLGVFKKIWPDVWSARMEYILNNTILSLLEVPGTTLLGINRML
                     SDKDYRKMIVDRITDPAIKSFWIREFAQYPPKFREEAIAPIQNKVGQFISSPMIRNIV
                     GQVKSTINMREVMDEKKILILNLSKGRIGEDPAKLLGALLITKLQLAAMSRIDIPEEE
                     RKDFFLYVDEFQNFATEAFVTILSEARKYRLCLTLAHQYIAQMEEVVRDAVFGNVGTI
                     IAFRVGAPDAEFLEKEFEPEINLYNLVNLPKYNIYLKLMIDGVTSRPFAAVTLPPLPK
                     PEKSYKEKIIKLSRERYGTEREKVEQKIARWMGNQETKKIIEREKEEKNLQKKEISLK
                     ELSNQKREEPDKNKLKKALEEAKKK"
     gene            10382..10873
                     /locus_tag="P5570_00920"
     CDS             10382..10873
                     /locus_tag="P5570_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HRR39309.1"
                     /translation="MSDYFQSPTYGKMSLNQVIDSLIDFILKNPDDIYQLIVGTDSEG
                     KEDPEFVTAIVLHRKGKGGRFFYQRSRTGKKYTLRTRIYEETMKSLTLAVKLRELLEK
                     HLQKNHPHNLKDLEIHTDIGQAGGTREMIKEIVGMIKGNGFEVKIKPESFGATSVADR
                     CLR"
     gene            10884..11375
                     /locus_tag="P5570_00925"
     CDS             10884..11375
                     /locus_tag="P5570_00925"
                     /inference="COORDINATES: protein motif:HMM:NF039497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5673 domain-containing protein"
                     /protein_id="HRR39310.1"
                     /translation="MRFNFFQKDKQLPDQKEVIFEWKNDVKEVIDKRWQIGISIFLII
                     CFVFFIMDKNYWGAGMVIVISFLIFLFPYYQKEQSFAILKNGIKIGNEVFPWDYLESF
                     WIIEEEPFEIYIKHKRKFPGNIILPFDKKDVNKIKHILSGYLPEQEIKRNFIDKVFRK
                     IGL"
     gene            11379..11450
                     /locus_tag="P5570_00930"
     tRNA            11379..11450
                     /locus_tag="P5570_00930"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11412..11414,aa:Arg,seq:gcg)
     gene            11533..13758
                     /gene="recG"
                     /locus_tag="P5570_00935"
     CDS             11533..13758
                     /gene="recG"
                     /locus_tag="P5570_00935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRR39311.1"
                     /translation="MKLDQPIESFRYVGPVYLKRLQRLGIKTIGDLFYHFPFRYDDFS
                     NVKKINELKIGEEVTIEGKIKEIKTRRSPVRKMFLTEALIEDESGAIKCIWFNQPYLA
                     QTLPKGTKARFAGKIKESKNSIFFSSPVYEIIESPSNHSNEATLIHTEGLVPVYPETE
                     GISSRWLRRLIYQLLQKYAQEIEEFLPEEILKKKGFPKISTAFFQIHFPRTKEEAEIA
                     KKRFAFEELFLIQLFYQKQKKNLKQKSAPKIIPKIEYIEKFKTNLPFSFTEDQKKVMR
                     EILDDMQKGVPMSRLLEGEVGSGKTVVAALVSFVTAKNGYQVAIMAPTEILAEQHFKE
                     LIKLFKGLDVSLGLLTSAGTRIFDKNQETLLTPQPKDENLDKKTKEEDFLKMIKKGAI
                     DIVIGTHSLIQERVIFKNLGLVVIDEQHRFGVEQRAKLLRNPKISNSQLNNDCLNQLI
                     PHSLSMTATPIPRTLALALYGDLSISQIKQLPKGRKKIITKVVSSRDSARNKVYEFLR
                     QEVKKGHQGFVVCPLIEESKKLARPSTKSQFGVYLSLVEVKAAEKEFKRLKEKIFPDL
                     RLGLLHGRMKQKEKEEVMRAFKNGEIQILVSTPVVEVGIDVPNATVMIIEGADRFGLS
                     QLYQFRGRVGRGEEQSYCFLFTESSAKRTKQRLNAIVSAENAFELAEKDLKIRGPGDF
                     IGTRQSGIPDLAMASLLDVSLVEEVKKEVEDILKKDPDFKNYPSLQKKMEEFKEKVFL
                     E"
     gene            complement(13812..13882)
                     /locus_tag="P5570_00940"
     tRNA            complement(13812..13882)
                     /locus_tag="P5570_00940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13848..13850),aa:Glu,seq:ttc)
     gene            complement(13894..14505)
                     /locus_tag="P5570_00945"
     CDS             complement(13894..14505)
                     /locus_tag="P5570_00945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRR39312.1"
                     /translation="MFPLYDETHQYRHKTPIVTSGLIFLNILIFLFTLFNLETVVEVF
                     GMVPQDILAGKNLQTIFTSMFLHSGFAHLIGNMWFLWIFGDNLESTLGKARFLFFYLL
                     CGVLAAVGYCFVTEEKNIPVIGASGAIAGILGGYFVIFPRNQIRTLIPIGFFFTTIRV
                     PAIIFLFIWIFSQFFLTDPNVATGAHIIGFFAGMTLVKLFQKR"
BASE COUNT         5212 a         2213 c         2456 g         4752 t
ORIGIN      
        1 tttattatat ttcttctaag ataactgttg caactttttt cgctgcatcg ggcatagcga
       61 actttttaca gccatttttc atctcctgaa gtgttttttt attttcaaga atttgatttg
      121 ctcttccaaa tacaaaatta ggagtaacat tgcatccctc aactgatatt gttgctccgt
      181 atttttgaaa acattgagca ttttttgatt ggtgatcttg cgctccaccc tgaagaggta
      241 ttaaaatact gggtttttca agggccgcta gttcaaatat tgcaccagcg ccagctcttg
      301 aaataacaag atcacttgcc tggtatgcgt cagctagttg ttcttcgtca agaaaaggat
      361 ataaatgata aaaaactttt tgcgcatcat ctaaagctcc tcttgtaaaa aggtctatat
      421 ttttaaaatt attaggaccg caaatatgaa ttatttcgta tttttcaata tattttaaaa
      481 gggcatcaag aaaaagagaa ttaatttgtt cagatccctg gcttcctccc ataataagaa
      541 tgatttttcg cttcccgttt aaattaaaat tttttatagc tttttctttt gaaccgtaaa
      601 ataatttttg tctgatggga ttgccagtaa gcactaattt tcttttatgc ttttttggaa
      661 aaaaatcttc tgattcctta aaagaaatag caatttttcg ggcaatggga gaagaaagtt
      721 tgcttgtgga gccaggtacg ctgtctgatt catggatcac gataggaatt tgatacaacc
      781 aacctactaa catcacggga aatgaaccag gtcctccctt ggaaaatatc aaatcaggca
      841 taataagcca aactttaaac aaggcctgta aaaaagaaat tggaaattta aaaatatcaa
      901 gaatgttttt aaaagaaaaa tatcgtcgcc actttacagt aacaattttt ttaaaagaaa
      961 tattttcttt taaaaggacc tctgtttctt gagttttgcc acctaaaaac tgaaaaagcg
     1021 aagattttat ttcttctggg acaagattat caatttctct tttaacagcg ataattggga
     1081 aaatatgtcc cccgcttccg ccgccggtaa ataagattcg cataaataaa tagatcaaaa
     1141 ataaaatatc aaaaatcaaa aaattgtttt tattttaaca ttacaatgga cttgatgcct
     1201 ttactatcaa aaaagcttta attttttttc aatataatag gcacagaaaa aataatcggg
     1261 gaaaaagcaa ttattaacgg tgcctcaaaa aagagagcaa aaataaaggt taacaaaaac
     1321 aaaatactag ttaaaaaatc acttgtttta aaatctttat ttttacccgg tatagaaaat
     1381 cttgattttt gttttaaaaa ataacttggc actgctgtaa taaaaccagc taaaagaaat
     1441 ggtatcggca aaagataaaa aagattacca aaggtagtag ccgctccttt tcctcccata
     1501 aaatgaagaa agatagacca attatgtccg ataattgcag cagctccagc caagaccatc
     1561 acccaccctt ctccaccaga cattctatca gcaatgataa ttgaaagtgc tcccttgcaa
     1621 aaatccatta ttcctaccaa agcgccagca aatttaccaa catttctact gactgatgct
     1681 gtgcccaatc tgccgttttc tacccttgag gtgatatcaa ttcccttatt tttagcaatg
     1741 agataagcag agggaataga acctaaaaaa taagcaatga taattggaat aattttacta
     1801 aacatttttt tggcacacta cttcaactga tgagtgaaaa aatcttttat aaattcctct
     1861 ccaattaaaa taggtaattt taaaattgtt aagtttaagt gcttcttcaa gatcttcttt
     1921 atcaaaaaga gcagcgggct tatcaaaata tttactgaaa ggaagattat aaaattttgc
     1981 taacggttct ctaaaaataa aataaccgcc tgacttcaat gttctactta cttcatttaa
     2041 tgcttttttc caatcaggaa tatgatgaag tgttgcaaac ataaaaattg catcaaaaat
     2101 ttcactatca aatggcagtt gttgagcatc agcaggcttt acttctgtat ttttaatttg
     2161 ttcttttaaa attctatttt gagcaatttt taacgaatct gtatcaatat caacaccaat
     2221 cattttttga ggagcaaaat attgatttat aattggtagt gcataaccgt ctttgcaacc
     2281 aatttcaaga aaataagcgt ttttaggaag ataaatttta gaaaaaaaac tttgaagtcc
     2341 tcccttttga acaatcttgc caaaggttaa attattaaat ttaactactg atgattgaaa
     2401 gctcatatta acaattctta atctgcttta ttattagaaa acggttttta ataaataaaa
     2461 taatttgcat taaggatttt accccctatc tttaattata tcatttttta tcttaactga
     2521 caaatttcaa attttggctt aattaattta attatttgaa taagatcttc ttttttgaga
     2581 actggcaagg aaaggatgtc aggttcaata atagcttttt gctcattcat ctcttgcaaa
     2641 aaatattttt ttgccgggcc tatccattga gcaatattaa taatgtcttc tttttctagt
     2701 ccaggggcaa cagtagttct aaattcataa tcaatcctat tttccttcaa aagattaata
     2761 cttttttcaa tcaatgataa atctattttt ttgttggtgt atatttcata tttttctttt
     2821 ggtgatttta tatccattgc tatataatcc actaattttt catcaattaa tttttttaca
     2881 atatccggat ttgagccatt agtatccaat tttaccaaaa aacctaattt tttaatcttt
     2941 tgacaaaatt gaggaagatc cttgtggata gttggttctc caccgcaaat acacacacca
     3001 tcaagtaatc ccttttttga tgttaaaaat tcaaaaaaat ctttttctga aattcttggt
     3061 tggtctttta ttttttctag caatacaagt tctgttgcat aacaaaaggg gcatctaaaa
     3121 ttgcacccaa tagtaaaaac cgtggctgca atgcagccag gataatcaat aagagtggtt
     3181 ttttgaagac ctccaatttc catataaagt taagaacctt gaatctcttt ctcttttttt
     3241 tcttctatct cttctatctt tttttcataa tcattgatta attcttgaag atcgtttttt
     3301 tgtctgaatt tttcatcttc aggtatttca cctttccttt ccatttcttg aatttttttc
     3361 caggattcct cacgtgtttg ccttaaagct accttcgctt tttccatttt ctgtcttacc
     3421 agttttatca ttttttcttt gtcctcagcg ctttgcatag gaagagaaaa aaatattttt
     3481 tctccttcgt ttcttacact tcctccaaga ttagaggcct gaagcgcttt ttcaatattg
     3541 ggaatagaag atttatccca aggttcaata gaaatagttc gagcatcaag tagagaaagc
     3601 gaagcaactt ctcgaagagt aattttttct ccataatact caaccaaaat tccatcaatc
     3661 aactcaatag acactcttcc tcccctgatt attaatactt cttttttgaa ccaatcaaga
     3721 gttttttcaa aagaatcttt tgtattttta aataattgat ttgtgtccat atttttataa
     3781 taattatttt attaattaaa atgttttttt aacagaaagt aaaatagtat caggatctgc
     3841 gggattaatt tttatttttt caatcattcc cctggttggt ggttcaatta cttgccaatt
     3901 ttctccatcg ttttctgaaa gataaatcag atttcccatt cccacatata atatattaga
     3961 attttttggg tcaactgtaa aggcagtaat tttatttttt tctgacgatg aaactatctt
     4021 caccaggtcc atttcgtttc ggcttattct aaaaagataa taatctgata taagatagat
     4081 tcgattctga gaaggatcag taataatatc tttgatttca ggcagttcac caatttcatt
     4141 ttctacttta gaggtatcaa tttcttcttt gattaatttg aaggtttctc cactatccac
     4201 gctaatttct attttgctgg gaagtccgtt ggaataaaca ttaaaagggt ctcgagcaat
     4261 ggatgataat aaaatataaa tttctcctga ttgatgagga ttaagcacaa ttttttctat
     4321 gggttgagaa aatttatatg tttctttcca agaattgcca aaatcctcgc tttttaaaaa
     4381 agatccttgt tttgttccta aataaagacg attagaaaaa agaaagtgtc tggaataaaa
     4441 cggatcaata gcaaatgttt ttattttatc atcattgctg gcaataaaaa gggggtggaa
     4501 atccgccccc ttgttataag aaaccaatag tttattttga ccgacttctt cagaaacaag
     4561 ataaaacaca tcagggtttc gaggatcagt ggtaagattt gaaacaacgg ctggctggct
     4621 ttcagtttta aatgtagttg tttttgctgt aaatttttct cctttggttg agctttcaaa
     4681 aactcccttt gccgaagcaa gataaagatt ttgagagttt ttaggatcaa aaacaagatt
     4741 ttcaatactt gctttcttgt cagctttatc aatagatttc aaagcaaccc attcttctcc
     4801 cctgtcctca gagagaaaaa gttgctcgcc catggaaact tcaacaccgt aattattttc
     4861 taaaaagtta aaaacaccta taaaacaaag ataggcaaca aaaaaactaa ttagtgctaa
     4921 tccaagttgt tgaaacataa taaaaaaatc aaaaatcaaa agaattttaa aaattaatac
     4981 tttatatgcc gagcaaaagg ttttccattg ctaaaagcgg attggtatta gaaaaataaa
     5041 ggtaattttt tgtttctttt atcttgaaaa taatttcttt taattttatt tcagaaaaat
     5101 tttctttttc ctcttccaat tctttttcct ttgaaaaaaa tgaaggatag aaagatgaga
     5161 gaccttgttt tcttaaaagc aaatctcgaa accaaaattc tgttaagaaa agaaaatctt
     5221 gagttctttc ggtcttttca agatcttcag ctagttttaa tcgttgaaga atagacaaat
     5281 ttcttatttt ttttatctcc cctaataata aattataata tattattttg tttttatcaa
     5341 taagaaattc tttggcaaga cctggcctgc ccaagacaag acctattatt tcctctttct
     5401 ctttttggtc tggttggctc tttaaaaatg cttccatttc aattcgtgaa agcggataaa
     5461 atttaaacat ttctactcgg gaaaggatgg tagtaggcaa ataattagga gaagaagtta
     5521 aaagaaaaaa aatggtattg ccccggggct cctcaagtat tttaagaagc gatcctgtag
     5581 cttcttctgt taatgattca ctgtggtcaa taataacaat tttaaatgga tatggcgaag
     5641 aaagagagat ttgctgttta atatctctaa tttttttaat atgaatttgg cgatctttga
     5701 caaaattatt atccttgaca gaagattttt tttctttttt ttccttgtct attgttatta
     5761 ggtcataaaa atttttctct tctatctgtt tacaaatatc acaagaacaa ttaccctgag
     5821 aaaactcaaa aaaattatca aaagacgaag aatggtctgt ttctaaatat tttgcaattt
     5881 caagagcaat ttttctttta ccaatttttt ctggaccagc aaataaaacg gcatggggaa
     5941 accgattttc tgctatcatt ttagaaaaaa tctttagatt tttttggtga ccaaagatca
     6001 tatttaaatt ataaatgatt tttaaaaaat ttcaaaataa aaataaaagt taggttcaaa
     6061 aatttaatgc aatatttaca aataataaaa caccaaacag aaatcagttg tcagaaaaca
     6121 gaaaacagta gagaaaatag aaatcagaaa acagattttc tcttattatt tcttatattg
     6181 tcatcgcgac ccctcaccac tgtcatcgcg agcgaggtca aagaccgagc gtggcgatcc
     6241 cagggtattg cctcgggatt gcttcgtcgc tatcgctcct cgcaaagaca ataaggggga
     6301 cgaaactgct gcacttcgct gcggctcgtg tcaaagacag gctctttttt gtcatcgcga
     6361 ggagtccctc accttagtaa aaggtgagtg gacgacgtgg cgatctcgat gcattgttgc
     6421 gttaaaaatt tgaaactaaa taagatcagg gattgcttgc cttattttct tagttttttg
     6481 aagagatttt agttaattca gaaacattgt agcccagtta aaatttgaca aaatgcaaaa
     6541 ttttgagtat tctaaagaat aaatatgttt aatattaatg attgcaaaca aacagtagat
     6601 ggaattttaa aagagatgcc agatagaact caaaaggtat tatcgcgtcg gtttggcatt
     6661 gatcctgaaa ataaatcaac aaataggtgt tgtgaaaccc ttgaaagcat tggaaaagat
     6721 tttggcatta cccgggaacg agtgaggcag attgaaaata gggggctgga agagttcaaa
     6781 cgttcttcat actttgctga tgttcagcgt ttttttcttt ttcttcgaag ttttattgat
     6841 gagcaaggtg gtttaaaaag acaggatata ctggaaaaaa cccttgtacc agaagaacaa
     6901 ttaaggcctt atttattgtt tattttaaaa attagtaatt tgtttttatt tgagccagaa
     6961 tcatcttctt tctattcttc ttggaagacc agagaagagg tttctgatat tgcagatagg
     7021 gtggtgcgtt ttttaattga tttaatggaa aaagaaaaaa gattgttttc agaagaagaa
     7081 ttattagaac gaggcacaaa agaagtccca aaatacttgc atcttactat tcctactaat
     7141 catttgattt cttatattga agctacaaaa aaaataagaa aagatcaatt taataattat
     7201 ggaccggtat ggtggccaga agttaatcca agaggaaaca gagattatgc ttatatgatt
     7261 ttaaaaattg aaaaaaggcc tcttcatttt cgtgaaatag caaaaaagat tgaagaatct
     7321 tttcagagac ccgttcaagc aggcagtttg cataatgaat taataaaaaa tgataaattt
     7381 gttttagtgg gacgaggaac ctatggatta aaagagtggg gttataaaaa cggaactgtt
     7441 aaggatatta ttgaagatat tttaaaagaa aacggaccct taacaagaaa tgaggttttg
     7501 aatgaagtgc aaaaacaaag aatggtaaaa agagatacaa ttcttttaaa tctatcaagt
     7561 tttaaaaaaa caccagatgg aaaatacata ttataatcaa aaattaaaaa tcaaaagttt
     7621 tgatttttga tttttgatta tgtctcttta tcttgaagaa tttttaaatc ttttattatc
     7681 tctttggtgg ttatggctgg caattttgct atttccccta gcaaaatatt tttttgtgtg
     7741 ggcaaaaaaa gaccattggt ttggatctct taaatataaa atgctggaaa tcaaaattcc
     7801 agaagaaata gaaaaaaccc caaaagcaat ggaaaatgtt tttcattcaa tgtggggaat
     7861 gtatgatcct ccctccaaca ttagagatta ttggctggac ggaaaagata tgctttatta
     7921 cagctttgaa attgttggca gacaagacga ggttcatttt tatattatgg cgcccaatac
     7981 ccatgttcaa ttggtaaaat cttctatatg ggcagaatat ccagaggcgg aaattaagga
     8041 agtagaagat tatataagta actttggtaa agaagtgcct aacgaggatt atgatttatg
     8101 gggtacagat ttaaaattgg caaaaccaga tgcttatccc attagaacct atgattattg
     8161 ggaaacagaa ttaacaaagg atgaaaaaaa aattgatcca ctggccgctc tttttgaggc
     8221 atttggcgat cttaaagagg gagaagaagt atgggttcaa ttaaaagttg cacctgtaac
     8281 agaagatgag cacccctatc ttgcagaaag tcaaaaagtg ataaataaga taatgaaaag
     8341 acctgaaact aagaaaaaag gcattttagc tcccttgggt ctttcaagaa ttccttctga
     8401 tgtgtggaca gttcttattg aaggaaaacc tattccacca gcaatggaag aagaattgcc
     8461 agatggattt gatattggat taatgaaatt aagtcctggt gaagcagagg ccctgcgagc
     8521 aattgaagat aatctaggaa aatatgttta tgaaggaaat gtccgatttt tatatattgc
     8581 caagtacgat attatttcaa aaccaagagg aataacaaca gtaatgtctg cgtttaatca
     8641 gtttgctact gttaatctaa attccttaaa accagacaga agcaaaactt cagtaattcc
     8701 atggtttttt gagggacgtc gtctttatgt taaaaagcgc cgtattttca gatattacac
     8761 aggaagagct tggccctggc atcgatcacc ttatattttt tctacagctg aactagccac
     8821 aatgtatcat ttccctggaa aaggagttgc gccttcaacc gctgttccgc gagtagagat
     8881 aaaaagagga gaaataccac ccaaccttcc tacttaaaaa ctttaaaaaa attatggaag
     8941 aaaaagaaaa agttgttcat tttgctaaaa cagtttatca tgacagagaa aaagaatttg
     9001 gaattaaaac cgatgataga cggcgtcata tttatatcgt aggaaaaact ggaatgggaa
     9061 aaagctgtct tttggagaat atggcaattc aggatattca aaatggtcaa ggtgttggca
     9121 tcgttgatcc ccatggagag ttagctgaaa aactgcttga ttttgtaccg aaaaatagaa
     9181 tcaatgatgt aatttatttt aatccagcag atcttgatta tcctattgct tttaatgtaa
     9241 tggaaaaagt agaatttact caccgtcatt tggtttcagc aggacttctg ggtgtgttta
     9301 aaaaaatttg gcccgatgta tggagcgctc gaatggaata tattttaaat aatactattt
     9361 tatctctgct tgaggtacca ggtactaccc tgcttggaat aaatagaatg ctgtcagata
     9421 aggattatag aaaaatgatt gttgatagaa ttactgatcc tgctattaaa tcattttgga
     9481 taagggaatt tgctcaatat cctcctaagt ttagagaaga agccattgct cctattcaaa
     9541 ataaagtggg tcaatttatt tcttcgccaa tgattaggaa tattgttggt caagtaaagt
     9601 ctactattaa tatgagagaa gtaatggacg agaaaaaaat tctcatttta aatctttcta
     9661 agggtagaat tggcgaggat cctgcaaaat tattgggagc actgttgatt acaaaacttc
     9721 aattggcggc gatgtcacga attgatatac cagaagaaga aagaaaagat ttctttttat
     9781 atgtagatga atttcaaaat ttcgccactg aagcgtttgt aaccattttg tctgaagcaa
     9841 gaaaatatcg gctttgtctc acccttgctc atcaatacat tgcacaaatg gaagaagtgg
     9901 taagagatgc agtatttggt aatgtaggca ctattattgc ttttcgtgtc ggagcccctg
     9961 atgctgaatt tttagaaaaa gagtttgaac cagagattaa tctatataat cttgttaatt
    10021 taccaaaata taatatatat ttaaaattaa tgatagatgg agttacgtcc cggccttttg
    10081 ctgcggttac tcttcctccc cttccaaaac ctgaaaaaag ttacaaagaa aaaattataa
    10141 aattgtctcg agaacgatat ggaacagaaa gagaaaaagt tgaacaaaaa attgctcgat
    10201 ggatgggaaa tcaagaaaca aaaaagatta ttgaaagaga gaaagaagag aaaaatctac
    10261 aaaaaaaaga aatttcttta aaagaattat ccaaccaaaa acgagaagaa cctgataaaa
    10321 ataaattaaa aaaagcgtta gaggaagcaa agaaaaaata aaaaggataa ttattgataa
    10381 tatgtctgat tattttcaaa gtcctactta tggtaaaatg agccttaatc aagttattga
    10441 tagtttgatc gattttattt taaaaaaccc tgatgatatt tatcaattaa ttgtgggtac
    10501 tgattctgag ggaaaagaag atcctgagtt tgttacagcg attgttttgc atcgtaaagg
    10561 caaaggagga cgatttttct atcaaagatc aagaactgga aagaaataca ctttgcgaac
    10621 tagaatttat gaagaaacaa tgaaatcttt aacattagca gtaaaattaa gagaattatt
    10681 ggaaaaacat ttacaaaaaa atcatcctca caacttaaaa gatcttgaaa ttcatactga
    10741 tattggacag gcaggtggca cgagggaaat gattaaagaa atagtaggaa tgataaaagg
    10801 aaatggtttt gaagttaaaa ttaaacctga atcattcgga gcaacttcag tagcagaccg
    10861 ttgtttgaga taaatattaa tatatgagat ttaatttttt tcaaaaagat aagcaattgc
    10921 cagatcaaaa agaagtaatt tttgaatgga aaaatgatgt taaagaagtt attgataaac
    10981 gatggcaaat aggaataagt atttttttaa ttatttgttt tgtttttttt ataatggata
    11041 aaaattattg gggagcggga atggtgattg ttattagttt tttaatcttt ttattccctt
    11101 attatcaaaa agaacaatca tttgctattt tgaaaaacgg cattaaaata ggaaatgagg
    11161 tgtttccttg ggattatctt gaaagttttt ggataatcga agaagaacca tttgaaattt
    11221 acatcaaaca taaaagaaaa tttccaggca atattattct cccctttgat aaaaaagatg
    11281 ttaataaaat taaacatatt ctttcaggtt atttgccaga acaagaaatt aaaagaaatt
    11341 ttattgataa agtttttaga aaaatcggat tgtaaaatgc cctcgtagtt taacggatag
    11401 aacaggggct tgcggagcct caaatagggg ttcgattcct ctcgagggca aaattaaatg
    11461 ataggatact tccctatcat tttttattta agcatctttt aataaaattt attatagatt
    11521 ataataatta ttatgaaact tgatcaacca attgaatctt ttcgttatgt gggacctgta
    11581 tatttaaaac gattgcagcg attgggtata aaaaccattg gtgatctttt ttatcacttt
    11641 ccttttcgtt atgatgattt ttctaatgta aaaaagatca atgaattaaa gattggtgaa
    11701 gaagtaacaa ttgagggaaa aataaaagag attaaaacta gaagaagtcc tgtaagaaaa
    11761 atgtttttaa cagaggcatt aatagaagac gaaagtggag caataaaatg tatttggttc
    11821 aatcaaccct atcttgctca aaccttgccc aagggaacaa aagcgcgttt tgctggcaaa
    11881 ataaaagaat ctaaaaatag tattttcttt tctagtccgg tttatgaaat aattgaatcc
    11941 ccttctaatc attcaaacga agctactctt attcataccg aaggtttggt tccagtgtat
    12001 cctgaaacag aaggaataag ttctcgctgg ttacgccgat taatctatca attattgcaa
    12061 aaatatgctc aagaaataga agaattttta ccggaagaga tattaaaaaa gaaaggattt
    12121 cccaagatca gtacagcttt ttttcaaatc cattttccta gaaccaaaga agaagcagaa
    12181 attgctaaaa aaagatttgc gtttgaagaa ctttttttaa ttcaactttt ttaccaaaaa
    12241 cagaaaaaaa atcttaaaca aaaatcagca ccaaaaatta ttcctaaaat tgaatatatt
    12301 gaaaaattta agacaaacct tcctttttct tttactgagg atcaaaaaaa agtaatgaga
    12361 gaaattttag atgatatgca aaaaggagtt ccaatgtcac gcttattgga aggagaggtg
    12421 ggttctggaa aaactgtagt agcggccctt gtctcttttg taacagcaaa aaatggttat
    12481 caagtagcta taatggctcc cactgaaatt ttagcagaac aacatttcaa agaactaata
    12541 aaattattta agggacttga cgtttctctt ggattattaa ctagcgctgg cacaagaata
    12601 tttgataaaa atcaggaaac cttattgacg ccccagccaa aagatgaaaa ccttgataaa
    12661 aaaacaaaag aagaagattt tttaaaaatg ataaaaaagg gggcaattga tattgtgatt
    12721 ggcactcata gtttgattca agaacgggta atttttaaaa atctaggttt ggtagtgatt
    12781 gacgagcaac atagatttgg cgtggaacaa cgagcaaaac ttttacgtaa tcccaaaatt
    12841 tcaaattctc aattaaataa cgattgttta aaccaattaa ttcctcattc tttatcaatg
    12901 acagcgacac caattcctcg tactttagct ttggctcttt atggagatct ttcaatttct
    12961 caaattaaac aattgccaaa gggcagaaaa aaaattatta caaaagtagt tagttcaaga
    13021 gattcagcaa gaaataaggt atatgaattt ttaagacaag aagtaaaaaa aggccatcag
    13081 ggatttgtgg tgtgtcccct catcgaagaa tctaaaaagt tagctcgtcc atcaacaaaa
    13141 tctcagtttg gagtttatct ttcattggta gaggtaaaag cggcggaaaa agaatttaaa
    13201 cggctcaaag aaaaaatttt tccagatctt cggttaggtc ttctgcacgg cagaatgaaa
    13261 caaaaagaaa aagaagaagt aatgagggca tttaaaaacg gagaaattca aattttagta
    13321 tcgacgccag tagtggaagt aggcattgat gtacccaatg ctacagtaat gattattgaa
    13381 ggagcagatc gatttgggct atctcaatta tatcaattta ggggcagagt gggacgagga
    13441 gaagaacaaa gttattgttt tctatttaca gaatcttctg ctaaaagaac caaacaaaga
    13501 ttaaatgcta ttgtttctgc tgaaaatgct ttcgaattgg ctgaaaaaga tttaaaaatt
    13561 cgtggacctg gtgattttat cggaacacga caatcaggaa tacccgattt ggcaatggct
    13621 tctttactag atgtttcctt ggtagaagaa gtaaaaaaag aagttgaaga tattttaaaa
    13681 aaggaccctg attttaagaa ttatccttct ttacaaaaaa agatggagga attcaaagaa
    13741 aaggtattct tagaataaaa aaaaacaccc tttgcggtca aggggtgttt ttaactaatt
    13801 taataaaata acggcgcata tgggagtcga acccatcttg ccagattgaa agcctggtgt
    13861 cctgccgata gacgaatgcg ccagtaaagt attttacctt ttttgaaata attttacaag
    13921 tgtcatacca gcaaaaaatc caataatatg tgcgccagtt gcaacattag gatctgttaa
    13981 aaaaaattgg gaaaagatcc aaataaaaag aaaaataatg gctggaactc taatagtagt
    14041 aaaaaaaaat ccaataggaa ttagggttct aatttgattt cgtggaaaaa ttacaaagta
    14101 tccacctagt attccagcaa tagctcccga tgcaccaatc acaggaatat ttttttcttc
    14161 agtaacaaaa caataaccaa cagcagctaa aacaccacat aaaagataaa aaaataaaaa
    14221 acgtgctttt cctaaggttg attctaaatt atcaccaaaa atccacaaaa accacatatt
    14281 gccaataaga tgggcaaatc cactgtgtaa aaacatagag gtaaaaatgg tttgaagatt
    14341 ttttcctgcc aaaatatctt gaggtaccat tccaaatact tcaactactg tttctagatt
    14401 gaataaagtg aaaagaaaaa tgagaatatt taaaaaaatt aatccactag taacaattgg
    14461 agttttatgc ctatactgat gagtttcatc ataaagagga aacataaact tttattttgt
    14521 aaaagaagtt ccaagattaa aattctaaat acaaaataaa tcttattatc gcttgccgcg
    14581 ctgcgctacc gtttaacgtg atacggtaaa cgttggcgtt gcgcgtaaac tat
//
LOCUS       DAOTQD010000011        14259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_4170_length_14259_cov_8.704520, whole genome shotgun sequence.
ACCESSION   DAOTQD010000011 DAOTQD010000000
VERSION     DAOTQD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14259
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_14259_cov_8.704520"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="P5570_00950"
     CDS             complement(<1..551)
                     /locus_tag="P5570_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39313.1"
                     /translation="MENSKLMQNTINKIKKLFSKKINIKKYYFRPKFILAVAMVLAAG
                     VGLYQVFAQVSTVNITSPTASAKAYVKSGDQVPVYFDVTTNASGTGRLRVEIYSGSTT
                     VGDTGYYDYTFLEGANNSLLANVLINASAAEGTYNVKVSAQQPGVSGTWVYDTETGAI
                     VVDNTPPTVTISDDQTGIASDADP"
     gene            complement(1606..1947)
                     /locus_tag="P5570_00955"
     CDS             complement(1606..1947)
                     /locus_tag="P5570_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39314.1"
                     /translation="MEITKKDLKEILEEQEKKYENYLGSVAEDFKSQVQLIAEQYGSI
                     KETLDSHTETLSSHTEMIASIKEDIEIMKIDIQFIKGSLKQKVDVEEFEALARRVALL
                     EAKMSKTNPPR"
     gene            complement(2461..3792)
                     /locus_tag="P5570_00960"
     CDS             complement(2461..3792)
                     /locus_tag="P5570_00960"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRR39315.1"
                     /translation="MFLFDLIKNIYHFLWAFFSALFCGFPSRHLKVIGITGTKGKSTV
                     SEMISKILEEADFRVASLDSIKIKVDKNEEENLFKMTTPGRGYLQTFLKRATKAHCHY
                     AIIEITSEGILQHRQKFINFDTVVFTNLSPEHIERHGSFENYRAAKGKLFKENKKNHI
                     LNLDDANIDYFLQFPAEKKIGFTLKDSPKGADKIIKGKDLKIKEMGSEFFVEIYSSHY
                     EPQQSNPETPASNQNEITVKDKSSSEKIELKLPGVFNVYNALCAIAIGFSQNVSLSIC
                     KRALEKIEGVPGRMEMVIKEPFKVIVDYAHTPDSLKEVYETLRNQNSKLICVLGSCGG
                     GRDKWKRPKMGEIAAKYCDEIIITNEDPYDEDPLQIMKQVASGIKSKKHYLILDRREA
                     INKALSLANTNDVVVITGKGCEPWMCVANGKKIPWDDREIAREEFKKISHF"
     gene            complement(3859..>4322)
                     /locus_tag="P5570_00965"
     CDS             complement(3859..>4322)
                     /locus_tag="P5570_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR39316.1"
                     /translation="NIRKRSKELRYIVKCQISKVKSQIFISNLKSFMESNDYKKEFYK
                     RLYKYALKIIKFVEKLPKDTASKIIGSQLIRSGTSVTSNTIEAKSASSKKDYINYYRT
                     ALKSANESKLWICLLRDTEKLNKKESDELLKETVEIANILAASILTMKGKK"
     assembly_gap    4323..4422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4423..9173)
                     /locus_tag="P5570_00970"
     CDS             complement(<4423..9173)
                     /locus_tag="P5570_00970"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR39317.1"
                     /translation="MEKNKKTSDYNAKDIYVLEGLEPVRRRPGMYIGSTGPEGLHHLI
                     FEVADNSLDEATMGFATEIEISLLPGNRISVSDNGRGIPVDVHPQTKKSALETVMTYL
                     HAGAKFGGKTYRASGGLHGVGVSVVCALSKWMRAEVSRDGEKYFQEYERGKPITKLTK
                     MGKTDQSGTTIIFEPDPEIFKKIEFSLKKIIDHLRLQAYLIPKLKIRIKDERKTPPFI
                     YTFYFEGGIKSLLRWLCQGEKILQENIFYGKKQVNDIQVEATFCYTEDIESEEMSFAN
                     NILTREGGTHLTGFRAALTRTIQNWGRQNKLLTDKDKIEGEDVREGLTAIISVKLPEP
                     EFEGQTKQKLGNPEARSAVETMISDLLPDFLEKNPRDGRMIIEKCLLAARSRQAAKNA
                     KESVLRKGLLEGLALPGKLTDCTSKNPEESELFIVEGESAGGCLAGNTKVALTDGRNL
                     TFKELIREDKKGKKNYCYTIKKDGSIGIGLIKYPRKTRENAKVIKIILDNKEEIICTP
                     DHLFMLKDGKYKKAKDLKSNDSLMPLYRKFSRIKDGFKIDGYEMVYEQKRHKWIYTHF
                     LANKYNLKNKKCKSPKKFCIHHKDFNKLNNNPDNLVQMDKQKHLELHAGIIKETMARP
                     EIQEKIRAVHQSKEYREKVRATMLKPDMRKKLSERAKKQWENEEYKQYMVQKFLEFYQ
                     KNPEYRERNNKLLYENQEKYWSDLKNRENWAQKVKEYFKNYPEKKKNLSLKAKEQWEN
                     EELLEWRSQKTKEQWTQEFRIKRKKTYNETYKEKALSLMRKIFEKYGEIDKEKYNQER
                     LKLKDKSILRFDTICQRFFANDEEKLEEAVLNYNHKIKKIIPLKKKIDVYDLEVENTH
                     NFALSSGIFIHNSSRQGRDRRTQAILPLRGKILNVERVHLNRVLSSKEVKSLIIALGT
                     AIADDFDINKLRYHKIIIMADADSVTGDMPILLFNKETQEYFLTEVEKFIENCDDTFK
                     YQVLTYNSKTKKREQKEICETIKHPLRTPLFEIKTCCGYSIKVTSCHSIYVFENGKVI
                     TKKGSEIKKGDLLIFPKSFPRGDREYLIDLQKTILNSNYENISVKVSKDEVNQIPAMA
                     WCELNSSFWSSFQKERELVGISRKKMGKEIQVYDKVIQQWEQKIDNVMPRFYQFQSYL
                     EKLNIEPSSLKYDVYIPLNEIKKQELPQSAKFYLKNHTHEIRTQFKLDKDLAYLIGWF
                     LGDGCFSPEKKSPNRFTVVLGKEKAENYIKELSRIIKEKFGAKTIIEYKKPNAIQLHF
                     HSFEFRLILTQLGLLGKKAEEKFIPDIFFNVKKEIQEGLLQGLLQSDGFITVWQKKNN
                     IRKAIYGWRLASQKLIEGTLTIFRQWGIFPSYSVFQGKDHLRKDGKIIRSNFKSYDLA
                     ISTVDYLLQTKNIWEGHKDAQKLENYFKKVNYKKAVGKNIQPISQDFVGLPVKEVKEI
                     KNPEDKFIYDFSVVGDQNFIAGTGGILCHNTDGNHIRTLLLTLFYRYFKPIIEKGYLY
                     IAQPPLYKIQCGKKVEYAYTDEEKEEVLKKIGNQPVSIQRYKGLGEMNAEELWETTME
                     PEKRILRQVTIEDAEEADRVFDILMGKEVLP"
     gene            complement(9214..11445)
                     /gene="pheT"
                     /locus_tag="P5570_00975"
     CDS             complement(9214..11445)
                     /gene="pheT"
                     /locus_tag="P5570_00975"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRR39318.1"
                     /translation="MIFSYNWLQSFFKEKLPRPEKLAELLTMHSFEVEEVKKIPNSKF
                     QIPKSKAVKDYLLDIDVLPNRGCDCLSHLGIAREIAALLKKSKIQNPKSKPKEDKNLK
                     TKDFIKVEVKDKNACKRYTARFMAGVNISVSPQWMQNRLRACGLQSINNVVDALNYVM
                     LEMGQPLHAFDYDKISNNPPIKSNGARILENSSKKNIIIRFAAKGEKIVTLDKKNYQL
                     DEDILIIADEKQPLALAGIKGGKIPEIDKNTKNLVIESANFDQRIIRYASKKLNLRTD
                     ASTRFEQKIDPNLTEEAINRAVFLIQQLSKGKVAEGMIDIYPKKEFPKKIKLDLDYVE
                     SLLGIKISQKEIIKILKSLGLKIVNCKLKTLNVVVPTLRTDISIPEDLVEEIGRVYGY
                     EKIQPQSPILPLKVPGKNRDLLWEIKIKEMLKGLGLNEVYLYSFISERDAELFSIRKN
                     SPLPLIEIKNPASSNTKYLRPSLLSNLLKTIERNQAFLKKEIRIFEIGKTYHWNVKSG
                     KLKVGKLPSTSDSLHSNIVEKKMLAGVITGNNKFFELKGILEGIFEGLGMVDVFFDEF
                     RATPEETPQIFWQAGESAEIKIGDIEIGFLGKIRPEILNFYNINKTPIPSQNDEAVEV
                     FAFEIDFKKLLNLLEEEHEYKMPSPYPESIKDIAVLVPSQTLAGEVIQTIHQIDEENL
                     IRDVEIFDIFEGSPLPKGKKNLAFHIVFQANDRTLSSKEIDAILNKIIDGLEENEEWK
                     VRR"
     gene            complement(11494..11877)
                     /locus_tag="P5570_00980"
     CDS             complement(11494..11877)
                     /locus_tag="P5570_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRR39319.1"
                     /translation="MDETKIIYPELSYKIIGVAFKIFNEIGFGMSEKFYQKAFAKELD
                     KEKISYEKEKCIKLNYQDQNIGSYFLDFLIEDKIIVELKVRPRFGYVHIRQVLSYLRS
                     SGYKLAILIYFTRDGVKYRRIINTK"
     gene            complement(11963..12973)
                     /gene="pheS"
                     /locus_tag="P5570_00985"
     CDS             complement(11963..12973)
                     /gene="pheS"
                     /locus_tag="P5570_00985"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRR39320.1"
                     /translation="MSLILIKNQAEKEIDAAKELKDLDAVWQKYFGKKGKISLLFKGI
                     KNLPQEEKKQMGRELNEAKNEIEKKIEKKKNQLLDYLRSKSRSFKSIDVSLPGNKIPQ
                     GHLHPLTKVLFECQEIFEKLGFEVVEGPDIESEWYNFDALNIPAWHPARDMWDTFWLK
                     EPGDSKLKDNSQKYLLRTHTSPVQVRYMEKHNPPIRIVVPGRCFRFEATDASHEIQFY
                     QLEGLMVDKDISLANLKAILIEFFRNFFGREIEMRFRPGYFPFTEPSIEVDINFEGRW
                     MELLGAGMVHPNVFKNAGLNPKDCQGFAFGVGIDRLAMLKYKINDIRLFYSGDLRFLE
                     QE"
     gene            complement(13030..13102)
                     /locus_tag="P5570_00990"
     tRNA            complement(13030..13102)
                     /locus_tag="P5570_00990"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13067..13069),aa:Lys,seq:ctt)
     gene            complement(13107..14042)
                     /locus_tag="P5570_00995"
     CDS             complement(13107..14042)
                     /locus_tag="P5570_00995"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRR39321.1"
                     /translation="MQKKAIIMGFVMGVFVLLFLPNSFLLKAETPFPGACSSFPQKGS
                     DGTCSGMGGGLNIDNLCRGSINDCNDKEYDVCCKLPDGRYVHYHTSCKTTNDSTLGSI
                     CECNKNDAWYEVCSIGCSGNLCASTSGSDDGGSGSGGSGSGGGGSGSGGSGGGGSGGS
                     GVSYTKVECKPEQQGGLVPCGRSCDDPDTTINEQAPCTFCHFLVLFKRIIDFLTTIIA
                     FPVLALMIIAGGIMFLTSSGNEKQLNKAKEILKTAVIGVVIILCAWLVIDTIITILTP
                     GDSPLQSWNEINCPFCGDGSCESPENSRNCPYDCY"
     gene            complement(14137..14208)
                     /locus_tag="P5570_01000"
     tRNA            complement(14137..14208)
                     /locus_tag="P5570_01000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14173..14175),aa:Arg,seq:tcg)
BASE COUNT         4200 a         2656 c         2090 g         5213 t
ORIGIN      
        1 ggatcagcat cgctggctat accagtctgg tcatcagaaa tagttactgt tggcggagtg
       61 ttgtcaacaa ctattgcgcc tgtttcagtg tcgtaaaccc aagtgccgct aactccgggc
      121 tgttgggcag acacctttac attataagta ccttcggcag cagaagcgtt gattaaaaca
      181 ttggcgagca aactattatt tgcgccttca agaaaagtgt aatcgtaata accggtatct
      241 cccacagtag tagagccaga ataaatttct actcgtaatc ttcctgtacc ggaggcattg
      301 gtagtaacat caaaataaac aggaacttga tcgcctgatt ttacataagc ctttgcgctg
      361 gcggtcgggc tggttatatt gacagttgaa acttgagcaa agacttgata taagccaact
      421 cccgccgcca aaaccattgc aacggctaaa ataaattttg gtctaaaata atatttttta
      481 atattaattt ttttgctaaa taattttttg attttgttta ttgtattttg cataagtttt
      541 gagttttcca taagtttagt ttttttgctc ttaacgttca agtaggtcga ccgtttataa
      601 ttgtgtatgt ctttgcgagc ccgactaccc ttcttgtcat cgcgagagag atcgaagatc
      661 gagcgtggcg atcccgtttt attgttgcgg gcttcgcccg agattgcttc gtcgctcacg
      721 cgagccccgt aagaaatctt tgatttcgca cggggcgaag cgttgaaaag aagataaaat
      781 cttctttgaa cgctcctcgc aatgacagtg gtggggggga ttgcttcccc ctcacccttg
      841 tgaaggtgag gggtcgcaat gacattatga cactaatctc tacttttcta aacgctttga
      901 gccattaaaa aatcccgcac taaaacgcgg ggtttttgat gacctttaaa aaaatatttg
      961 ttgttgttat tataaaaaag aataacattt tacctccaaa aatattaatt aattataata
     1021 tattaatata ataaattttt tttaaaaaat caatattcaa tttttaatgt tcaatgttca
     1081 atcaaattct aagtttggca aaaccaaata aaaggtgaaa aaagatttat ctctttaatc
     1141 ttaagaatct tatattcatt ataattataa aaaataaaaa gtcaaatctc taaaaaattt
     1201 caatgatcaa atttctggaa tttgaaattc ttaaattctt aattctattt taatcttata
     1261 aaattttttg tcaagggaat aaaataaatt ccaatattca atttccaagc tcggcgaagc
     1321 cgagcgaagg gcgaaaagaa caaagttctt taagctctaa atcctaatgt tcaatggtgt
     1381 tgtacacttt ctgtttagaa ttttgaacat tagaatttga ttggtcattg gttattggag
     1441 cttggaaatt tatgtaataa taattttgct ctaagaatgc ttcgcattct tttcgccctt
     1501 ctctttgtct ataatttatc aatgtagaga aggaactgat ttttttattt tacgcaagtt
     1561 tgggaaaact ttaagagatt gttaagggct gtaactttta aataattatc ttggtgggtt
     1621 ggttttgctc atttttgctt caagcaaagc caccctgcgg gctaatgctt caaattcttc
     1681 tacatctact ttttgtttca acgaaccttt aataaattga atatctattt tcattatttc
     1741 tatgtcttcc tttatagaag ctatcatttc agtgtgggaa cttaatgttt cggtatgaga
     1801 gtcaagggtt tctttaattg aaccgtattg ctcggcaata agttgaacct gggatttaaa
     1861 atcctctgcc acgctaccaa gataattctc atattttttc tcttgctcct ctaaaatttc
     1921 ttttaaatct tttttagtaa tttccataaa tttattttat cacaaaaata atttatcaac
     1981 aagggctatg ataattcagt gggaattatt tcaaattcca atgttcaatt ttaaatccta
     2041 aacttaattt tcaattttaa attattaaat ttctctactg tctttgcgag aagtgttcaa
     2101 agaagatttt atcttctttt caacgcttcg ccccgtgcga aatcaaagat ttcttacggg
     2161 gctcgcgtga gcgacgaagc aatcccgtgg taatactctg ggattgccac gtcctactgc
     2221 ggtaggactc gcaatgacag tagatgcgca acgagatcgc caccccctca ccttcacaaa
     2281 ggtgagggct cgcgatgaca gtggtagttt tctaatttct aatttctgtt tagaatattg
     2341 gtcattagaa tttgattggt cattggttat tggagcttgg gattttatta taaactattt
     2401 taactatttc tttattttgc gcaaggtttt agagaaattt aaaggattgt taaggaacta
     2461 ttaaaagtga cttatttttt tgaactcttc tcttgcaatt tccctgtcgt cccagggaat
     2521 cttttttcca tttgcaacgc acatccaagg ttcacatccc ttgccagtaa taactactac
     2581 atcatttgta tttgctaaac tcaatgcttt atttatcgct tctctccggt ctaaaatcaa
     2641 atagtgcttc ttagatttaa tgcctgatgc cacttgcttc ataatttgaa gaggatcttc
     2701 atcataaggg tcttcgttgg taataataat ttcatcgcaa tactttgcgg caatttcacc
     2761 cattttgggc cttttccatt tgtctctgcc accaccacaa gaaccaagaa cacaaattag
     2821 ttttgagttt tgatttctca aggtttcata aacctcttta agtgaatctg gtgtgtgcgc
     2881 ataatcaaca ataaccttaa acggctcttt aatcaccatt tccattctgc caggaactcc
     2941 ttcaattttc tccagcgccc ttttacaaat tgaaagtgaa acattttgag aaaatccaat
     3001 ggcaatggcg caaagcgcat tataaacatt gaaaacacca ggtaatttta attcaatctt
     3061 ttctgaagat gatttatctt ttactgtaat ttcattttga ttagatgcgg gggtttcggg
     3121 attgctttgc tgtggttcgt aatggctgga ataaatttca acaaaaaatt cactccccat
     3181 ttctttgatt tttaaatctt ttccttttat tattttatca gccccttttg gagaatcttt
     3241 taaggtaaag ccaattttct tttctgctgg gaattgtaaa aaataatcaa tatttgcatc
     3301 gtctaagttt aaaatatgat tttttttatt ttctttgaaa agtttgccct ttgccgcccg
     3361 gtagttttca aaagagccgt gtctttcaat gtgctctggt gaaagattgg taaaaactac
     3421 tgtatcaaaa ttaataaact tctggcggtg ctgtaaaatt ccttcggaag taatttcaat
     3481 aatagcgtaa tgacaatgtg cctttgttgc tctttttaaa aatgtttgaa gatatcccct
     3541 gccaggcgtt gtcattttaa aaagattttc ttcttcgttt ttatcaacct taatttttat
     3601 tgagtccaaa gaagctactc tgaaatctgc ttcttctaat atttttgaga tcatttctga
     3661 aacagttgat ttgcctttgg tgccggtaat tccaattact tttaaatggc gggagggaaa
     3721 gccgcaaaaa agagcgctga aaaaagccca aagaaagtga tagatatttt ttattaaatc
     3781 aaaaaggaac atagcgtaga attaatctca aatgttaaat gtcaaatctc aaaattacat
     3841 cttaaaaatc aaatcttatt atttcttacc tttcatagta agaatacttg cggcaaggat
     3901 gttggcgatt tcaactgttt cttttaataa ttcgtctgat tcttttttgt ttaacttttc
     3961 tgtgtctctc aaaagacaga tccataactt tgactcattt gcagatttta atgctgtcct
     4021 atagtagtta atataatctt ttttagagct ggctgatttt gcttcaattg tattactggt
     4081 aactgaagta ccgcttctga tgagctgaga tcctattatt tttgaggcag tatcttttgg
     4141 taatttttct acaaatttaa taatttttaa agcgtattta taaagtcgtt tataaaattc
     4201 ttttttataa tcgttacttt ccataaaaga tttaagattt gagatgaaga tttgagattt
     4261 tacttttgag atttgacatt ttactatata tctaagttct ttacttcttt tgcgtatgtt
     4321 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttggcaa cacttctttg
     4441 cccattaaaa tatcaaaaac tctgtcggct tcttcggcat cttcaatcgt tacctggcgc
     4501 aaaatacgct tttccggctc cattgttgtt tcccaaagct cctcggcatt catttctcca
     4561 agccccttgt atctctgaat agaaactggt tggtttccta tttttttcaa aacctcttct
     4621 ttttcctcgt cggtataagc atattctacc tttttaccac actgaatttt ataaagaggg
     4681 ggctgagcaa tgtaaagata ccccttttca atgattggtt taaaatagcg gtagaaaagc
     4741 gttaaaagca aggtgcgaat atgattacca tcggtattat gacaaagaat tccgcctgtg
     4801 ccggctataa aattttgatc gccgacaact gaaaaatcgt aaataaattt atcttctggg
     4861 tttttaattt ctttaacttc ttttactggc aaaccaacaa aatcttgaga aatcggctga
     4921 atattctttc caaccgcttt tttataattc acttttttaa aataattttc taatttttga
     4981 gcgtctttat gaccttccca aatattttta gtttgaagca aataatcaac tgtagagatt
     5041 gccaagtcat aggacttaaa attagatctg ataattttgc catcctttct taaatgatct
     5101 ttcccttgaa aaactgaata tgatggaaat attccccact gtctaaagat tgtaagagtg
     5161 ccttcaatta gcttttgaga agccaagcgc caaccataaa ttgcctttct tatgttattt
     5221 ttcttttgcc aaacagtaat aaaaccatcg ctttgtaaaa gcccctgaag cagaccttct
     5281 tgaatctctt tttttacatt aaagaagata tcaggaataa atttttcctc ggctttttta
     5341 cccaagaggc caagctgagt caaaattaat ctaaactcaa aagagtgaaa atggagttga
     5401 atagcatttg gttttttata ttctataatt gtctttgccc caaatttttc tttaataatt
     5461 cgagaaagtt ctttaatata attttctgct ttttctttac caagcacaac agtaaagcga
     5521 tttggacttt tcttttctgg agagaaacag ccatcaccta aaaaccatcc aatcaaataa
     5581 gctaaatctt tgtctagttt aaattgggtt ctgatttcat gagtgtggtt ttttaagtaa
     5641 aatttagcgc tttgcggaag ttcctgcttt tttatctcgt ttaaaggaat gtagacatcg
     5701 tattttaaag aggatggttc aatattgagt ttctctaaat agctttgaaa ttgataaaat
     5761 cgcggcatca cattatcaat tttttgttcc cactgctgaa taactttatc gtagacctga
     5821 atttcctttc ccattttctt acgagagatt cctaccagtt ctcgttcttt ttggaaagaa
     5881 gaccagaaag aagaattaag ttcgcaccat gccattgctg gaatttgatt tacttcatct
     5941 tttgaaacct tcacagaaat attttcataa tttgaattta aaattgtttt ttgtaagtca
     6001 attaaatatt ctctatcccc tcttggaaaa gattttggaa agataagtaa atctcctttt
     6061 ttaatctcgc ttcctttttt agttatcacc ttgccatttt caaaaacata aatactatgg
     6121 caggaagtaa cttttattga ataaccgcaa caagttttta tttcaaaaag aggcgttcgt
     6181 aatgggtgct ttatcgtctc acaaatttct ttttgttctc tttttttggt tttggaattg
     6241 taagttaaaa cttgatattt aaaagtatca tcgcaatttt caataaattt ttccacttca
     6301 gttaaaaaat attcttgggt ttctttatta aaaagcaaaa ttggcatatc tccagtaaca
     6361 gaatccgcat cagccattat gataatttta tggtatctca gtttattaat atcaaaatca
     6421 tcggcaatgg cagtacccaa agcaataatt aaagatttaa cttcttttga agacaaaaca
     6481 cgattcaaat gaaccctttc aacgtttaaa attttccctc gcaaaggcag aattgcttga
     6541 gtgcgtctat cccggccttg gcgtgaacta ttatgaataa atatacccga agacaaagca
     6601 aaattatgag tattctcaac ctctaaatcg taaacatcta tttttttctt tagtgggata
     6661 atttttttaa ttttatgatt atagttcaaa acagcttctt ctaatttttc ctcatcattg
     6721 gcaaaaaatc tttgacaaat tgtatcaaat cttaaaatac ttttatcttt taattttaat
     6781 ctttcttgat tatatttttc tttatcaatc tctccatact tttcaaaaat tttcctcatc
     6841 aaactaaggg ctttttcttt ataagtttca ttatatgttt tctttctttt aattctaaat
     6901 tcttgagtcc attgttcttt tgttttttga gatctccatt ctaataattc ttcattttcc
     6961 cactgttctt ttgcttttaa agaaaggttt tttttcttct cgggataatt cttaaaatat
     7021 tcttttactt tttgggccca attttctcta tttttaagat cactccaata tttttcctga
     7081 ttttcgtaaa gaagtttatt gtttctctcg cgatattcag gatttttttg gtaaaactct
     7141 aaaaatttct gaaccatata ttgtttatat tcctcatttt cccattgttt ttttgctcgc
     7201 tcgctaagtt tctttcgcat atctggtttt aacatcgttg ctctaacttt ttccctatat
     7261 tctttggatt gatgaactgc tcttattttt tcttgaattt cgggtctcgc catcgtttcc
     7321 ttaattatcc ctgcgtgtaa ttctaaatgt ttttgtttat ccatctgcac taaattatca
     7381 ggattgttat ttaatttatt aaaatcctta tgatgaatac aaaatttttt gggggactta
     7441 cattttttgt tttttaaatt atatttatta gccaaaaaat gagtgtatat ccatttgtgt
     7501 cttttttgtt cgtagaccat ttcatagcca tcaattttaa aaccatcttt aattcttgaa
     7561 aacttcctat ataaaggcat caaagaatcg ttggacttaa gatcctttgc ttttttgtat
     7621 tttccatctt ttaacataaa aagatggtca ggagtacaaa taatttcctc tttattatct
     7681 aaaatgattt ttataacctt agcgttttcc cgagttttcc ttggatattt tattaaacca
     7741 attccgatac ttccatcttt ttttattgta taacaataat tcttctttcc ttttttatct
     7801 tctcttatta attctttaaa tgttaaattt cgaccatcag ttaaagcaac ttttgtattt
     7861 ccagccaaac atcctccagc actttctcct tctactatga acaattctga ttcttcagga
     7921 ttttttgaag tacaatcagt aagtttgccg ggcaatgcta atccctctaa aagtcctttt
     7981 ctcaaaactg actctttggc gtttttagct gcctgtcttg atcttgctgc caataagcat
     8041 ttttcaataa tcattctgcc gtcgcgagga tttttttcca aaaaatcagg caaaagatca
     8101 gaaatcattg tttctactgc tgaacgcgct tcgggattgc ctaatttttg tttggtttgg
     8161 ccctcaaact caggttctgg taattttact gaaataattg ctgttaatcc ttctcttaca
     8221 tcctcgccct cgattttatc tttatcagtt aatagtttat tttgccgtcc ccagttttga
     8281 atagttcttg taagggcagc gcgaaaacca gttaggtgcg ttccgccctc gcgagtaaga
     8341 atattattag caaatgacat ttcctcggac tcaatgtctt ctgtatagca aaaagtcgct
     8401 tctacttgaa tatcatttac ttgtttcttc ccataaaaaa tattttcttg taaaatcttt
     8461 tctccttgac acagccaacg aagcaatgat tttatccctc cttcaaaata aaaagtatat
     8521 ataaaggggg gagtttttct ttcatctttg atccttattt ttaatttggg aataagatat
     8581 gcctgtaaac gcaaatgatc aataatcttt tttaaagaaa attctatttt tttgaaaatt
     8641 tcaggatctg gctcaaagat tatggtggtg ccggattgat cagtctttcc catttttgtt
     8701 aattttgtaa tgggttttcc tctctcatac tcttgaaaat atttttcccc gtcccttgaa
     8761 acttctgccc tcatccattt tgatagggcg caaacaactg aaactccaac accatggagt
     8821 ccgccactgg cacgatatgt ttttcctcca aattttgccc cggcatgaag atatgtcata
     8881 actgtttcta gggctgattt ttttgtttgg ggatgaacat caactggtat tccgcggccg
     8941 ttgtcagata cactgatacg atttcctggc aagagagaaa tttcaatttc tgtagcaaac
     9001 cccatcgtcg cttcgtccaa agaattatca gcaacctcaa aaattaaatg atgcaagccc
     9061 tcagggccgg ttgaaccaat atacattccg ggccgacggc gaaccggctc aagaccttct
     9121 aaaacataaa tatctttagc attataatct gaagtttttt tatttttttc catataaaag
     9181 cggggtaaat taaaaattta aaatttttag tatttatcgt cttaccttcc attcctcgtt
     9241 ttcttccaaa ccatcaataa ttttattaag tatagcatca atttcttttg aagataaggt
     9301 cctgtcattg gcttgaaaaa caatatgaaa tgctaaattc tttttgccct tgggcaatgg
     9361 cgatccttca aaaatatcaa agatctcaac atctctaatc agattttctt catctatttg
     9421 atgaatggtt tgaattacct cgcctgccag ggtttgactt ggaactaaaa cagcaatatc
     9481 cttaatgctt tccggatagg gcgagggcat tttatattcg tgttcctctt ccaacaaatt
     9541 taacaatttt ttaaaatcaa tttcaaaagc aaatacctca acggcttcat cgttttggga
     9601 aggaatagga gttttattaa tattataaaa atttaaaatt tctggtctta tctttcctaa
     9661 aaatccaatc tcaatatcac caatttttat ctcagctgat tcgcctgctt gccaaaaaat
     9721 ttgtggggtt tcttctggtg ttgccctaaa ctcatcaaaa aagacatcaa ccattcccaa
     9781 tccctcaaaa atcccctcta atattccttt taactcaaaa aatttattat taccagtaat
     9841 aacccctgcc agcattttct tctctacaat gtttgaatgt agagaatcgg aagtggaagg
     9901 tagttttcct actttcaact ttccactttt aacattccaa tgatatgttt ttcctatctc
     9961 aaaaattcta atttcttttt ttaaaaatgc ctgatttctt tctattgttt ttagcaagtt
    10021 tgataaaagt gacggccgta aatatttagt attggaactg gcaggatttt taatttcaat
    10081 taagggtaag ggcgaatttt tacgaataga aaaaagttct gcatctctct cagaaataaa
    10141 agaataaaga taaacctcgt taagccccag tcccttcaac atttctttta tttttatttc
    10201 ccaaagtaaa tccctgtttt taccaggtac cttaagaggc aaaataggac tttggggctg
    10261 aattttttca tatccataaa ctctacctat ctcctctaca agatcctcgg gtattgaaat
    10321 atcggttctt aatgtgggta caaccacatt tagagttttc aatttacaat ttacaatttt
    10381 caaacctaat gattttaaaa ttttaattat ttccttctga gaaattttta ttccaagcaa
    10441 gctttcaaca taatctaaat ccaacttaat ctttttggga aattcttttt taggataaat
    10501 atcaatcatt ccctctgcta cttttccctt ggataattgt tggatcaaaa acacagctcg
    10561 attaatggct tcttctgtca gattggggtc aattttttgt tcaaatcttg ttgaggcatc
    10621 tgtcctcagg tttaattttt ttgaggcata acgaataatt ctttgatcaa aattggctga
    10681 ttcaattaca agatttttgg tatttttatc aatctcagga atctttccgc ccttaattcc
    10741 agccaaagcc aagggctgtt tttcatcagc gattattaaa atatcttcat ccaactgata
    10801 atttttttta tcaagcgtta caatcttttc tcctttagca gcaaaccgaa taattatatt
    10861 ttttttcgaa ctattttcaa gtattcgagc cccattcgat ttaattggtg ggttgttcga
    10921 aattttatca taatcaaaag catgaagggg ctgtcccatt tctaacatta cataattcag
    10981 ggcgtcaacc acattattta tggactgaag cccacaagcc cttaatctat tttgcatcca
    11041 ctgtggagaa acagaaatat taaccccagc cataaaacgc gctgtatatc ttttacaagc
    11101 atttttgtcc ttaacttcca cttttataaa atcttttgtt tttaaatttt tatcttcctt
    11161 cggtttggat tttggatttt ggattttgga tttttttaat aatgctgcta tttctcgtgc
    11221 aattcctaga tgagaaagac aatcacatcc tcggttgggc aaaacatcga tatctaaaag
    11281 atagtccttt actgcttttg attttggaat ttggaatttg gaatttggaa ttttttttac
    11341 ctcttcaact tcaaaactgt gcattgttaa aagttcagcc aatttttcag gccttggaag
    11401 cttttcttta aaaaatgatt gtaaccaatt ataagaaaaa atcatatgga gtaatcacga
    11461 atatacgaat atacacaaat atacgaatta cgactatttt gtatttataa ttcttcgata
    11521 cttcacacca tcacgcgtaa aataaataag tattgcaagt ttatatccac ttgaacgtaa
    11581 atatgataaa acttgtctta tatgaacata accaaatcgt ggtcttacct ttaattcaac
    11641 gataatttta tcttcaatta agaaatctaa aaaataactc ccaatatttt gatcttgata
    11701 atttaatttt atgcattttt ctttttcata agaaattttt tccttatcaa gttcttttgc
    11761 aaatgctttt tgataaaatt tctcactcat cccaaatcca atttcattaa atattttaaa
    11821 agcaacacca ataattttat aactcaactc aggataaata attttcgtct catccataaa
    11881 tttgtatatt attttaaatt cgtatattcg tgattactcc attcatgaat ttgtatatta
    11941 ttttgaattc gtatattcgt gattactcct gctctaaaaa ccttaaatct cctgaataaa
    12001 ataatctgat atcattaatc ttgtatttta acattgctaa tctgtcaatc cctactccaa
    12061 aagcaaatcc ttgacaatct ttaggattta atccagcatt tttaaaaaca ttgggatgta
    12121 ccattcctgc gcccaacaac tccatccatc gtccttcaaa attaatatct acttcaatac
    12181 tgggctcagt aaaaggaaaa taccctggac gaaaacgcat ttcaatttct ctgccaaaaa
    12241 aatttctgaa aaactcaatt aatattgctt ttaaattagc taaagaaata tctttatcca
    12301 ccattaatcc ttctagctgg taaaattgaa tttcatggga ggcatcggtt gcctcaaagc
    12361 gaaaacatct tcctggcaca acaatcctta tcggcggatt gtgtttttcc atataacgta
    12421 cctgcactgg cgaggtatgg gtacgaagta agtatttttg gctattgtct tttagtttgg
    12481 aatctcccgg ctctttgagc caaaatgtgt cccacatatc ccgagcagga tgccacgctg
    12541 gtatattaag agcatcaaaa ttataccatt cgctttctat atcagggccc tcaactactt
    12601 caaatcctaa tttttcaaag atttcctgac attcaaaaag caccttggtt aagggatgaa
    12661 gatgaccttg cggaatttta tttccaggca agcttacatc aattgattta aaagaccggg
    12721 actttgaacg caaataatct aataattgat ttttcttttt ttctattttt ttctcaattt
    12781 cattttttgc ttcatttaat tccctaccca tttgtttttt ttcttcttga ggaagatttt
    12841 taatgccctt aaaaagcaat gaaatctttc cctttttgcc aaaatatttt tgccaaaccg
    12901 catctagatc ttttaattcc ttagcagcat ctatttcttt ttccgcttga tttttgatta
    12961 aaataagaga cataaatttt gcgttaaacg cttgcataac gcatttttat taatagggta
    13021 attttatttt gcccccagca ggactcgaac ctgcaaccaa tggcttaaga ggccactgct
    13081 ctaccaattg agctatgggg gcaggattaa tagcaatcat aaggacaatt tcgggaattt
    13141 tctggtgact cacaacttcc atccccacaa aagggacaat ttatctcatt ccaactttga
    13201 agaggactat cgccaggagt taaaatagta ataatggtat caattaccaa ccaagcacat
    13261 agaataatta caacaccaat tactgctgtt tttaaaattt cttttgcctt attaagctgt
    13321 ttttcatttc cagatgatgt taaaaacatt atgccccctg ctataatcat taaagccaac
    13381 actggaaaag caataatagt agttaaaaaa tcaataatcc ttttaaacaa aactaaaaaa
    13441 tgacaaaaag tgcaaggcgc ttgctcattt atcgttgtat cgggatcatc acagctcctt
    13501 ccacaaggca ctaatcctcc ttgctgttct ggtttgcatt caacttttgt atatgatact
    13561 cctgagccgc ctgagccacc gccgccagaa ccgccacttc cagaaccacc gccgcctgag
    13621 ccgctaccgc ctgagccgct accgccatca tcacttccac ttgttgatgc gcaaagattg
    13681 ccactgcatc cgattgaaca tacttcatac caagcatcat ttttgttgca ctcacaaatt
    13741 gatcctaaag ttgaatcgtt tgttgtttta caactagtgt gatagtgaac atatctacca
    13801 tctggtaatt tacaacaaac atcatattcc ttatcattac aatcattaat acttcccctg
    13861 cataaattat caatattcag tccgccaccc attcctgaac acgttccatc ggatcctttt
    13921 tgggggaaag aagaacaagc tccagggaaa ggtgtttcag ctttcagcaa aaaactattc
    13981 ggcaaaaaca ataaaacaaa aacacccatc acaaaaccca ttattattgc ctttttttgc
    14041 atatgcttta aactcttaat taagagttta ttattttttt gaaatattgt caaataaaaa
    14101 cagggatttt aaccctgttt acttatactg ctaacttgtc cccggaggga atcgaacccc
    14161 cattactagt tccgaagact agtgctttat ccgttaagct acggggacat tcagaattaa
    14221 aaattaatta ggttcaagaa tttaacgcaa cactcttta
//
LOCUS       DAOTQD010000012        12595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_4795_length_12595_cov_9.893461, whole genome shotgun sequence.
ACCESSION   DAOTQD010000012 DAOTQD010000000
VERSION     DAOTQD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12595
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_12595_cov_9.893461"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1654)
                     /locus_tag="P5570_01005"
     CDS             complement(41..1654)
                     /locus_tag="P5570_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRR39322.1"
                     /translation="MKQETNNKIKYFLYARKSSESEDRQVQSIDDQVNRLKKLADDLN
                     LDIKKIYTEAKSAKKPNNRPFFDEMIKRIENGEADGILCWHLNRLSRNPIDSGKLSWL
                     LQQRILKSIQTIERQYLPDDNVLLFNVESGMANQYILDLSRDTKRGMLKKLNAGWQTG
                     IAPLGYLNDKENKIIIKDPERFNLIRKMWDLMLTGNYTPPKILNIANNEWGFRTRKFK
                     RIGGCPLSRSGIYKIFTNLFYAGIIENAGMQYQGAHEPMITLEEYDRVQMILGRKGKP
                     RPKHHEFAFTGSIRCGSCGCLYTAETKRKFLKSGEIKEYTYYHCTRKTTKVNCDQRYN
                     INAKDLDLMIEKEIEKYTILPEFLQWALEGLNKKNDAEIEDRTKVYEMQHKTLVETQK
                     ELDELTKMRYRQLIDDETFIREKNELQTKITEMKENLRQTETRAEKWLELTEKTFNFA
                     TYARKAFLMADGKTGLELKKEILMALGKNPIIKDKKLYIEPNEWLQPIKNDYPALEAE
                     YLRLEPAKMPINKAKTEDLTSVRSRWLRW"
     gene            complement(2117..4285)
                     /locus_tag="P5570_01010"
     CDS             complement(2117..4285)
                     /locus_tag="P5570_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRR39323.1"
                     /translation="MEKLQTITIKEYLTRKGIPFKEQGGELITHCLFNNCDNDSRDGE
                     AHLYFDTETGQYNCKKCGAKGNIITLAEYLGDNINDIVLNPQKSRENIQKYPKFDASL
                     VEICHQAIPAHIRQYLNARGITDALINDFKLGWGEFYGKWWITIPIKDINGNFVFFKL
                     RQDPNEGDEKITYPRGIEAQIYDWETLKTATDKIVICEGEFDRLILLSKGISAITSTH
                     GAMTFKQEWCKKLVNVSKIYICFDNDETGKKGAERVAKMVKNDENKTYIITLPEEVGE
                     SGDITDYFTKLNGNSDDLFDKYAKEYPEKIDTSQFKPLSSSELIKILGLTIKKDEENK
                     LITFLCELSAYTENSQFNISFNAPSSTGKSYIPTEVARLFPEEDVIEIGYCSPTAFFH
                     DIGEYQKEKNGYLVDLSRKILIFLDQPHTQLLERLRPLLSHDKKEINLKITDKTQKFG
                     LKTKNVLLRGYPSVIFCTVGLKIDEQEATRFLLLSPEINQEKIREAIHEKIKKETDAD
                     TYQKWLDDNPERKLLKERIRAIKQENIREIKIGSPEKIEERFFKKNKMLKPRHSRDIG
                     RLISLIKSFALLNLWFREKDGSAIVANDDDIEEAFKIWEATSESQELNLSPYIFDLYK
                     NVILPLFEEKNQIAATEIVNEAGILRQEIIQKNYQLNGRLIPDWELRLHIIPMLENAG
                     LIRQEADPNDKRKMLIYPTTSLTISKEQNNSELNGGVNKI"
     gene            complement(4305..4502)
                     /locus_tag="P5570_01015"
     CDS             complement(4305..4502)
                     /locus_tag="P5570_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39324.1"
                     /translation="MLSDEQITKYQTLYKNRFGKEISREEAYEQGAKLIRLIELIYKP
                     MTENEYKQLQKRRRQTGDLKI"
     gene            complement(4507..4800)
                     /locus_tag="P5570_01020"
     CDS             complement(4507..4800)
                     /locus_tag="P5570_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5659 domain-containing protein"
                     /protein_id="HRR39325.1"
                     /translation="MTKPLKNGQPEEYQYIPLDDQTNYFYTFDLGCSAALISAGFELV
                     SLDKANPRKVQFIFRRKVGIEKVADDYWTDRLEVKARTFFDNVKMLKNRIYSE"
     gene            complement(4947..7136)
                     /locus_tag="P5570_01025"
     CDS             complement(4947..7136)
                     /locus_tag="P5570_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR39326.1"
                     /translation="MWLDEKSITLTPQWAKQFGIIPEKDKRGNPFDISFKSIKKDKNK
                     ISVDFKIKDDEKKSLLKSVSYKIKELTPYQLIFIGWAYINLDDYKNNYKEIAKRYLIE
                     AIEKNVDSCLPYYLLASENLKQYENWEIMELYQATEKPDVFPEFYRLLWRKEKNRIRA
                     EATIKKGVERFPHNLKLALHYAEILFNKNKPAEALRILKKGQPFEIEQGIDYDGSDYD
                     NLIYLTSSIYVALKKYQDAQRIIQKSSLSNDEQNFLLGLVNFKQNKYKRAVKAFTKAV
                     EEDYPNSNITKASLYYLLNCYHRLGQKKEVEKIIDLLPKGGGWFIFPFHHPFAEFAEQ
                     TLRLILKNCSSNERLKSKVSGFLAAVILDNKFLQEEENLKKRKLTKTEKRLLENTINL
                     AKEAVRLHPDDGFFNLILSNLVGINGDYDNSFYQGLYALNEPIGQYDLDDNHNLEKCS
                     EKFIEEYPEKLASVYNEIHFPIKNYIDIYLGSDIDWLYKRKKYQIISSLYNWVKSKND
                     WIEFGKQLEENKKELGEYAGGGLFEIAYSLNENEERDEAKKVYEYLVQLSGETPAVMN
                     NLAMIYEKNGDLSKAKELIKKAKSLLGTKDDEIIDRNFARLIKHSKKHKNTAKNSKKE
                     EEIKNKKSLPTFNVGNGEIVFGNKKCQIPLGSNQYQLCKEIFEKPLGEYVKEIDVIFN
                     FYRYSDSQRSFYDAVRLVNQKVEKAIGIKKLIEFKAASARIRKEIFE"
     gene            7209..7436
                     /locus_tag="P5570_01030"
     CDS             7209..7436
                     /locus_tag="P5570_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39327.1"
                     /translation="MLSNKSITKNTKNNKEMPELITLQEACDILKCHHNTLRQWDKKG
                     ILVAVPFWREKRQALSQRRYFEIIKPKEKMT"
     gene            7646..9532
                     /locus_tag="P5570_01035"
     CDS             7646..9532
                     /locus_tag="P5570_01035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRR39328.1"
                     /translation="MTKKLQLQSIEKAKGRPMLQWVGKKPLDKIEYYPAQEKEIYGDK
                     NSKDFNKLFWGDNLQVLAHLLKEYRGKINLIYIDPPFDSKAEYIKKVKIRGEKLEGEQ
                     QSLLEEKQYTDIWEKDEYLQFMYERLLIMRELLADTGSICLHADWHKGEYLRLLMDEV
                     FGDENFQNEVIWYFTNGTMKNVTKAFANQHNNLFIYNKNKNLHLFNELREENISQNLE
                     TRFGKYANEEGQIFYGDVKGKEKSLLERVVPRFIREYGREPQDEDVIYELQGIKLKSV
                     WNIPQVRGAEYPTEKPPELISRIIRAFSNEGDLVADFFVGSGTTPAVAQKLSRRWIAC
                     DINLGAIQTTAKRLNQIIQEQQTPKQSKLATEQEERAKDFKGLYTFKIYNVNDYDIFK
                     NEIEAKEIVMEMYGVEPIKRSYFDGTLDNNFVKVMPMNRVLNKMDIKDVFKNIENKID
                     TFSVRQKSKSGEPVYEEGVLIICSGMEFDVSDYLKKENKTGVKVEIRDILTDKKNLIF
                     KKKPEAKIEVKVKDKKLSVEIKEFYSPILMRKLEIENAKILQKEYQAKVKDFKQIIDS
                     VAIDVDYDGKIFNAEITDLPSKKEIIKSKYDWDYEQKGKYSIAVKVIDVLGEEYFETF
                     EINI"
     gene            9537..9755
                     /locus_tag="P5570_01040"
     CDS             9537..9755
                     /locus_tag="P5570_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39329.1"
                     /translation="MFTDLLSTYFILVAWKKNPSLTKRQIGVMHQWKEDTEENNKPLT
                     KDEVEGMKSFLKKYNPTAYYKLFPEEKQ"
     gene            9758..12310
                     /locus_tag="P5570_01045"
     CDS             9758..12310
                     /locus_tag="P5570_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRR39330.1"
                     /translation="MENPFLQHIQQKVAIWRDDNYKGVEKETLNILTHIKRVGFLHKP
                     QVEALETYIYLKEIVGNKPSLEVFKSLFENKKDMLLGLDISKEQAFDLMDDDKKIAEL
                     MEDRFGVSDYPNQVYALTMGAGKTILMAVMMVYDFVLSFYHPEDKRFAKNALVFAPDT
                     TIIESLKEIKTFDYTRVIPKEYQNILLNIKYHYLEDVATPLSPIGNYNVIVSNSQKII
                     LKTQRNHNNTQRLFSDWQYMANREIENSRLQAIKQLTDLIIFVDEAHHSYGKTLEGTL
                     KKTRQTINYIHQEGKTPIVDVINLTGTPYVNNKMIADVVYHFGLKQGIEKGILKQIRF
                     FEYSKVKTEKFIEDVVDKFWSEYGENRLEGRLPKIAFYSASIDDLQKDLRPKLEKILA
                     QRKIALDKILEYHTEAEENKDEFVAVDTIDSRKQFILLVGKGTEGWNCRSLVACALYR
                     KPKSAIFVLQSSTRCLRSIGDNSTLASIFLSEENYRILDKELRNNFATDIEELSAQDQ
                     KSIEYTLKIEKKKKLKVKKTLKEILAVQNQDLDKIKIDFKNFELKNYLSFIAEGGIFL
                     DEHKEAGYKEARIEKEVREMGDFTFYEIIEIINRYTHLSCLAIEQILENNGLSKENIV
                     EKVNKNPALLLFIMQNVLAEAYQYQEKTETIEEELELAKISTEEFKLNIEKRKEALVV
                     YREDYEKEIGEKTRLGFHINPYNYDSADEKNLFGYLQKYLEENEAIIDIYFTGGLTDQ
                     THNDFYFEYYSPEKERIARYFPDFLIETNKGRYLVIEVKSNRERITYEENKQQYLAGK
                     KKIFDEVFAKEIGFREFQQANKNFEYRIIFDASFRKHQQELLESIRKSIREI"
BASE COUNT         3984 a         2034 c         2203 g         4374 t
ORIGIN      
        1 gagcggctcc ctgttaagga gatggttgta ggttcgaatc ctaccaccgg agccagcgtg
       61 aacgaacaga ggttaaatcc tctgtttttg ctttatttat gggcattttg gctggttcta
      121 accttaagta ttctgcttct aatgcaggat agtcattttt gataggttga agccactcat
      181 tcggttcaat atagagcttt ttgtctttaa ttattgggtt ttttccgagt gccattaaaa
      241 tttctttttt cagttctaac cctgttttac catctgccat taaaaacgct tttctggcat
      301 aggttgcgaa attgaatgtt ttttctgtca gctcaagcca tttctcggct cgtgtttcgg
      361 tctgtcgtag attttctttc atttcggtaa tttttgtttg aagttcgttt ttctctctaa
      421 taaaggtttc atcatcaata agctggcggt atctcatttt tgtaagttcg tctaattctt
      481 tttgagtttc aaccagagtt ttatgttgca tttcgtaaac ttttgttctg tcttcaattt
      541 cagcgtcatt ctttttattc aatccttcca atgcccattg tagaaactct ggcaagatag
      601 tgtatttttc tatttctttt tcaatcatca aatctaaatc ttttgcattg atgttgtatc
      661 tttggtcgca atttactttt gttgtttttc ttgtgcagtg ataataagtg tattctttta
      721 tttcgccaga tttaagaaat ttcctttttg tttcggcggt ataaagacaa ccacaagagc
      781 cgcatcgaat agaaccagta aaagcaaact cgtggtgttt gggtcttggt ttgccttttc
      841 taccaagaat catttgtact cgatcatatt cttcaagagt aatcatcggc tcgtgtgctc
      901 cctgatattg cattccagcg ttttcaataa ttcccgcata aaacaaattt gtaaaaattt
      961 tataaatgcc gcttcgtgaa agaggacatc caccaattct cttgaacttt cttgtcctaa
     1021 agccccactc attgtttgct atatttaaaa tctttggcgg agtgtaatta ccagtcagca
     1081 ttaaatccca cattttacgg ataagattaa atcgttctgg gtctttaatg ataattttat
     1141 tttctttgtc gttgagataa ccaagtggtg ctatccctgt ttgccacccc gcatttaatt
     1201 tttttaacat tcctcgtttg gtatcacgac ttaaatcaag aatatattgg ttagccattc
     1261 cgctttctac attaaataac aacacattat catcgggcag gtattgtcgt tctatggtct
     1321 ggattgattt aagtattctt tgttgtaaaa gccagcttaa ttttccacta tcaataggat
     1381 ttcgagataa tctatttaag tgccagcata aaatcccatc agcttcgccg ttctcaatcc
     1441 gttttatcat ctcatcaaag aaagggcggt tattcggttt ttttgctgat ttcgcttcag
     1501 tataaatttt cttaatatcc aaattcaaat catcggcgag ttttttaaga cgattgactt
     1561 gatcatcaat agattgtact tgtcggtctt cgctctccga tgattttctg gcataaagaa
     1621 aatatttaat tttgttgttt gtttcttgtt tcatataact caaagggacg gtctggtagt
     1681 ccaatcgctc caccacgagg gttagcttac tggcagaccg cccctttggg gtcagtaaac
     1741 ttataagaaa ctcgtggtta cattgcgatt tggactacat ttacattata cactaaatct
     1801 aattgtcaag caaatttttt ccctcaatta ttccttctgt tttggcaggc tcaattatat
     1861 ttattctttt taattttatc tgttaatata agcaagtttt atacggcttt agttgactta
     1921 atttttttaa ttttcctacc tttttacact tttcgcactt ttttaacagt tttttaacag
     1981 ttttaacagt tattttttaa aatcactcaa aaataattta aaacttttca tatacaactt
     2041 tttgtttatt ttgtttattt tttaaaatca gttaaaaaat tagttatttt tttatttttt
     2101 tgttgttttt tcttactcat attttattta ccccaccatt caactcacta ttgttttgtt
     2161 ctttagagat agtaagcgag gtagtgggat agattaacat ttttctttta tcatttgggt
     2221 cagcttcttg tcggattaaa cccgcatttt ccaacatcgg tataatatgc aatcttaatt
     2281 cccaatcggg tatcaatcta ccattaagtt gataattttt ttgaataatc tcttgtctta
     2341 atataccagc ttcattaact atttctgtag ccgcaatctg atttttttct tcaaaaagag
     2401 gtaaaataac atttttgtat aaatcaaaaa tatacggcga taaattcagt tcctgacttt
     2461 cagaagttgc ttcccagatt ttgaaggctt cttcaatatc gtcatcgttt gctacaatag
     2521 ccgagccatc cttttctcta aaccataagt ttaatagagc aaaagatttt attaaggaaa
     2581 taagtcgtcc gatgtctctt gagtgtctgg gcttgagcat tttatttttc ttaaaaaatc
     2641 tttcttcaat tttctctggc gagcctattt tgatttccct gatattttcc tgttttattg
     2701 cccgtattct ttctttcaag agttttcttt cgggattatc atccagccat ttctgataag
     2761 tgtcagcgtc agtttctttt ttgatttttt cgtggattgc ttctctgatt ttttcctgat
     2821 tgatttctgg acttagtaaa agaaatcttg ttgcttcttg ctcgtcaatt tttaatccaa
     2881 cggtgcagaa aattactgac ggataacctc ttaacaaaac atttttggtt tttaatccga
     2941 acttctgggt tttgtcggta atttttaaat tgatttcttt tttgtcgtga gaaagcaaag
     3001 gtcttagtcg ttccaaaagt tgagtatgtg gttggtcaag aaaaattaaa atctttctgg
     3061 ataaatcaac taaatatcca tttttttctt tttgatattc cccaatatcg tgaaaaaagg
     3121 cagtcgggga gcaatagccg atttcaatga catcttcttc tggaaacaac cgagcaacct
     3181 ctgtggggat gtaggattta ccagttgatg acggagcatt aaaactgata ttaaactggg
     3241 aattttctgt ataagcggag agttcgcata agaaagttat caatttattt tcctcgtctt
     3301 ttttaatggt tagccccagt attttaatga gttctgatga ggagagcggt ttaaactgtg
     3361 aggtatcaat tttttctgga tattcttttg cgtatttatc aaataagtca tcagagttac
     3421 cattgagttt tgtgaagtaa tcggttatgt cgccgctttc accgacttcc tctggcaatg
     3481 taattatgta ggttttattt tcatcatttt ttaccatttt tgccacccgt tccgctcctt
     3541 ttttcccagt ttcatcattg tcaaaacaga tataaatttt gctaacattt actaactttt
     3601 tacaccactc ctgcttaaaa gtcatcgctc cgtgcgtgga agtgatagct gatattcctt
     3661 ttgataataa aatcaatcta tcaaattcgc cctcgcagat tacaatttta tcagtggcgg
     3721 ttttgagtgt ttcccagtca tagatttgag cttcaattcc tctgggataa gttatttttt
     3781 catcgccttc attcgggtct tgccttagtt tgaaaaacac aaaattccca ttgatgtctt
     3841 taataggaat ggtaatccac catttgccgt aaaactcgcc ccagccgagc ttaaaatcgt
     3901 ttataagtgc gtctgtaatg cctctggcgt tgagatattg gcgaatatga gctggaatgg
     3961 cttgatggca aatttctacc agagaagcgt caaatttcgg atatttttgg atattttctc
     4021 tgcttttttg tggatttaag acaatgtcat ttatgttgtc gccaagatat tcagcaagtg
     4081 ttataatgtt gccttttgct ccacattttt tgcaattgta ttgtccagtt tcagtatcaa
     4141 agtaaagatg tgcctctcca tctctgctat cattatcgca gttattaaat agacaatgag
     4201 ttattagttc tccaccttgt tctttaaaag gaatgccctt gcgggttaaa tattctttga
     4261 ttgtaattgt ttgtaatttt tccatatttt taatattaat ttagttagat ttttaagtcg
     4321 ccagtttgtc ggcggcgttt ttgtaattgc ttgtattcgt tttcagtcat cggcttataa
     4381 atcaattcaa taagtcggat gagtttcgca ccctgctcat acgcttcctc tcggctgatt
     4441 tctttgccga agcggttttt gtaaagcgtt tgatattttg ttatttgctc atccgaaagc
     4501 atagatttat tccgagtaaa ttcggttctt taacattttc acattgtcaa agaacgtcct
     4561 cgctttaact tccaagcggt ctgtccagta gtcatcagcg accttttcaa tgccgacttt
     4621 tcggcgaaag atgaactgga cttttcgtgg attggcttta tctaaagaca caagctcaaa
     4681 acccgcactt atcaatgcgg ctgaacaccc caaatcaaag gtgtaaaaat agtttgtctg
     4741 gtcatctaaa ggaatatatt gatattcctc tggttgacca ttttttaatg gtttggtcat
     4801 ataattttgg ttaagttaat aatgatcgac ctttgcggta cttctttggt tagaaacaaa
     4861 aaagtagcct tttcttttag gctactcttt tgattgcgag tcaagttaga tttacttgac
     4921 tctaaagttg attcaattga ttcgctttat tcaaaaattt cttttcgtat gcgagctgaa
     4981 gccgctttga actcaattag tttctttatg ccaatagctt tttctacttt ttggtttact
     5041 aatcgcactg cgtcatagaa gcttctttga ctatcagagt atcggtaaaa attaaatata
     5101 acatcaattt cttttacata ttctcccaat ggtttttcaa aaatttcttt gcaaagctga
     5161 tattgatttg agccaagcgg tatttggcat tttttatttc cgaatacaat ttcgccattg
     5221 ccaacattaa aagtgggcaa tgattttttg ttttttattt cttcttcttt tttagaattt
     5281 ttggcggtgt ttttgtgttt ttttgagtgt ttaataagcc gtgcgaaatt tctgtctata
     5341 atttcatcat cttttgtacc aagtaatgac tttgcttttt taataagttc ttttgcctta
     5401 cttaaatcac cattcttttc ataaatcata gccagattat tcataacagc gggtgtctct
     5461 ccagaaagtt gcactagata ttcataaact tttttggctt catctcgttc ttcattttca
     5521 tttagtgaat aagcaatttc aaacaatcca cctccagcat attctccgag ctctttttta
     5581 ttttcttcaa gctgtttacc aaattctatc caatcattct ttgatttcac ccagttatat
     5641 aggctggaaa taatttgata cttttttctc ttatataacc aatcaatgtc agaaccgaga
     5701 taaatatcaa tataattttt tattggaaaa tgtatctcgt tatagacgct tgctaacttt
     5761 tctgggtatt cttcaataaa tttttctgaa catttctcaa gattgtgatt atcgtctaag
     5821 tcatattgtc ctattggttc atttagagcg tatagacctt gatagaaaga attgtcataa
     5881 tcaccattaa taccaacgag atttgataag atgagattga aaaatccgtc atcagggtga
     5941 agtcgcacag cttctttagc aagatttata gtattctcta ataatcgttt ttcggttttt
     6001 gtaagttttc tttttttaag attttcttct tcttgcaaaa acttgttatc taagataact
     6061 gcggcaagaa aaccgctaac ttttgatttc aaccgttcat tagaagagca gttttttaat
     6121 atcagcctca gagtctgttc ggcaaattct gcaaaaggat gatgaaaagg aaaaataaac
     6181 catcctccgc cttttggtaa taaatcaata attttttcaa cctctttttt ctgtcctaat
     6241 ctgtgatagc agttaagtaa gtaatacagg cttgcttttg taatattact gttgggatag
     6301 tcttcttcta ctgcttttgt aaatgcctta actgctcgtt tatatttatt ttgtttgaaa
     6361 tttaccaaac cgagaaggaa gttttgctca tcatttgaca aacttgattt ttgaataatt
     6421 cgttgggcgt cttgatattt tttcaatgct acataaatag aggatgtcaa ataaataaga
     6481 ttatcataat cacttccatc ataatcaata ccttgttcta tctcaaaagg ctgacctttc
     6541 ttaagaattc ttaatgcttc tgcgggttta tttttattaa aaagaatttc tgcgtaatga
     6601 agtgctaatt taagattatg aggaaatctt tcaacacctt ttttaattgt ggcttccgct
     6661 cttattcgat ttttttcttt tctccataat aaacgataaa actctggaaa gacatctggt
     6721 ttttctgtcg cttgatagag ttccattatt tcccaatttt catattgttt cagattttcg
     6781 cttgctaaaa gataataagg caaacatgag tctacgtttt tttcaattgc ttctattaag
     6841 tatctttttg ctatttcttt gtaattattt ttgtaatcat ctaaattgat gtaagcccaa
     6901 ccaataaaaa ttagttgata gggagtaagc tctttaatct tgtaagaaac ggatttcaat
     6961 aacgatttct tctcatcatc tttaatttta aaatcaaccg aaattttatt tttatctttt
     7021 ttgattgatt taaatgaaat atcaaaagga tttccccttt tgtctttttc tggaataata
     7081 ccgaattgtt ttgcccattg aggcgttaat gtaatgcttt tttcatctaa ccacatagta
     7141 atttctggta attgtcattt aattaatgtt gcagattatt tttatctact atataataat
     7201 ggtataaaat gttaagcaat aagtcaatta caaaaaatac taaaaataat aaagaaatgc
     7261 cagagttgat tacacttcaa gaagcgtgtg atattttaaa gtgtcatcat aatactttac
     7321 ggcaatggga taagaaaggc attttagttg ctgttccgtt ttggcgagag aaaagacagg
     7381 cattatcgca aagaagatat tttgaaatta ttaaaccaaa agaaaagatg acataaatca
     7441 ttaacaattt aattgcctaa ccctcatagt tttctgtgag gtatggcaca aaaaatccat
     7501 tgcgacacag agacgggctc ccgtttcact actatgtcgc aatggttatc gccgaaaggc
     7561 ggtagacagt tccgtaagga ctgtcggtga gcggaagtcc ttttctgtta aaaaggcgac
     7621 cgctcataaa atccattgcg acaatatgac caaaaaatta caattacaat ccattgaaaa
     7681 agccaaaggt agaccaatgc ttcaatgggt aggcaaaaag cctttggata aaattgaata
     7741 ctatccagct caagaaaaag aaatttatgg cgataaaaat tccaaagatt ttaataaact
     7801 tttctggggc gataaccttc aagttttagc ccacctctta aaagaatatc ggggcaaaat
     7861 taatttgatt tacattgatc cgccgtttga tagtaaagcc gagtatatta agaaagtaaa
     7921 aatcagagga gaaaaattag agggcgaaca gcaaagtttg ttggaagaaa aacaatatac
     7981 cgacatctgg gagaaagacg agtatcttca atttatgtat gagcgtcttt tgattatgcg
     8041 tgaactcctt gctgatacag gaagtatttg cttgcatgct gattggcata agggtgagta
     8101 tttaagatta ctaatggatg aagtatttgg agatgagaat ttccaaaatg aagttatttg
     8161 gtattttact aacgggacaa tgaagaatgt tacgaaggcg tttgccaacc aacacaacaa
     8221 tctctttatc tataataaaa ataaaaatct ccatttgttt aatgaactcc gtgaagaaaa
     8281 catttcacag aatttagaga caaggtttgg caaatacgcc aatgaggaag gacaaatttt
     8341 ctacggagat gtaaagggca aagaaaagtc tctccttgaa agggtagtgc caagatttat
     8401 tagagaatat ggcagagagc cacaagacga agatgtaatt tatgagcttc aaggaattaa
     8461 attaaaaagt gtttggaata taccacaagt aagaggtgct gaatatccaa ctgaaaaacc
     8521 accagaattg ataagtcgta taataagggc attttctaac gaaggcgatt tagtcgccga
     8581 cttttttgtt ggctctggga caacgcctgc ggtagcacaa aaacttagca gacgatggat
     8641 tgcttgcgac atcaatttgg gagcaataca gacaactgcc aaacggctta atcaaattat
     8701 tcaagagcaa caaactccga agcagagcaa gctcgctacg gagcaagaag aaagagccaa
     8761 agattttaag ggtttataca ctttcaaaat ttacaatgtg aacgactacg atattttcaa
     8821 aaacgagatt gaagcaaaag aaatagtgat ggagatgtat ggcgtagagc cgataaaacg
     8881 aagttatttt gatggtactc tagacaataa cttcgtgaaa gtaatgccaa tgaaccgagt
     8941 actaaacaag atggatataa aagatgtgtt taaaaacatt gaaaataaaa ttgatacttt
     9001 ctcggtaagg caaaaatcaa aatcgggtga gccagtttat gaagaaggtg ttttgattat
     9061 ttgttctgga atggagtttg atgtgtcgga ctatttgaaa aaagaaaaca agacaggcgt
     9121 gaaagtggaa atccgagata ttttaactga taagaaaaat cttattttca agaaaaagcc
     9181 agaagctaag attgaagtaa aagtcaaaga taaaaaattg tcagtggaaa ttaaagagtt
     9241 ctactcaccg attttgatga gaaagttaga aatcgaaaat gctaaaatat tgcaaaagga
     9301 atatcaagcc aaagtaaaag attttaagca aattatagat agcgtggcga ttgatgttga
     9361 ttatgatggc aaaatcttta atgctgaaat tactgatttg ccgagcaaaa aagaaataat
     9421 caaaagcaaa tatgattggg attacgaaca aaaaggcaaa tatagcatag ctgtaaaagt
     9481 catagatgtg cttggtgaag aatactttga aacatttgaa ataaacatct aaatctatgt
     9541 ttactgattt attatcaact tactttattt tagtggcgtg gaagaaaaac ccctcactta
     9601 ccaaacgaca aattggagta atgcaccaat ggaaagaaga tacagaagaa aataataaac
     9661 ccctaacaaa ggatgaggtt gagggtatga aaagtttttt gaaaaaatat aaccctactg
     9721 catattacaa attatttccc gaagaaaaac aataattatg gaaaatccat ttttacaaca
     9781 catccagcaa aaagtcgcta tctggcgaga tgacaattat aagggcgtag aaaaagaaac
     9841 gctgaatatt ttaacccata ttaaacgagt gggttttttg cataagccac aagtagaagc
     9901 actagaaact tatatttatt taaaagaaat agttggcaac aagccgtctt tggaagtgtt
     9961 taagtctctt tttgaaaata aaaaagatat gcttcttgga ttagatattt ccaaagaaca
    10021 agcttttgat ttaatggatg atgataaaaa aattgccgag ttgatggaag accgctttgg
    10081 cgtttctgat tatcccaatc aagtttacgc tctcacaatg ggagctggca agacgatttt
    10141 aatggcagtg atgatggtgt atgattttgt tttgtcattt tatcatcccg aagataaacg
    10201 ctttgccaaa aatgctctgg tgtttgctcc agataccaca attattgaga gcttaaaaga
    10261 aattaaaact tttgactaca cacgagtaat tccaaaagaa tatcagaaca ttcttttgaa
    10321 tattaaatat cactatcttg aggatgttgc tacgccgttg tctccaattg gcaattataa
    10381 tgtgattgtt tctaactctc agaaaatcat tttaaaaacc cagagaaatc acaacaacac
    10441 tcaacgatta ttttccgatt ggcagtatat ggcaaatcgt gaaatagaaa acagtcgttt
    10501 gcaagccata aaacaattaa ctgatttaat tatttttgtg gatgaagccc atcattctta
    10561 tggcaagaca ctagaaggca cattaaagaa aacccgtcag acaattaact atatccatca
    10621 agaaggtaag acaccaattg tggatgtaat aaatcttact ggcacgccgt atgtaaataa
    10681 caaaatgatt gctgatgtag tttaccactt tggattaaag caaggcattg aaaaagggat
    10741 tttgaagcag ataagatttt ttgaatacag caaagtaaaa acggagaagt ttattgagga
    10801 tgtggtggat aagttttgga gcgaatatgg agaaaatcga cttgaaggac gcctgcctaa
    10861 aatagctttt tattctgcta gtattgatga tttacagaaa gatttaagac caaaactaga
    10921 gaagatttta gcccagcgaa aaatcgctct tgataaaatt ttagaatatc atactgaagc
    10981 cgaagaaaat aaagatgagt ttgttgctgt agatacaatt gacagcagga aacaatttat
    11041 tttacttgtt ggcaaaggaa cagagggttg gaattgccgc tcactcgttg cctgtgcttt
    11101 atatcgcaaa ccgaaaagtg ccatctttgt tttacaatct tcaactcgtt gcctacggag
    11161 cattggcgac aattcaacgc tggcttcaat tttcctttca gaagaaaatt atcgcatttt
    11221 agacaaagaa cttagaaata attttgctac tgatattgaa gaattgtcag cccaagacca
    11281 gaaatccatt gagtatactc ttaaaataga gaaaaagaaa aaattaaaag tgaaaaagac
    11341 cctaaaagaa attttggcgg ttcaaaatca ggatttagat aaaatcaaaa ttgatttcaa
    11401 aaacttcgaa ttaaagaatt atttgtcttt tatcgctgaa ggtgggatat ttttggatga
    11461 acacaaagaa gctggatata aagaagcaag aatagaaaaa gaagtgaggg agatgggcga
    11521 tttcactttt tacgaaatta ttgaaattat taaccgctac acccatttat cttgtttagc
    11581 tattgagcag attttggaaa acaatggatt gtcaaaagag aacatcgtag aaaaggtaaa
    11641 caaaaatcca gcacttctat tgtttattat gcagaacgtc ttggctgagg cttatcaata
    11701 tcaagaaaag acagaaacca ttgaagaaga attggagctg gcaaaaatta gcaccgaaga
    11761 gtttaagtta aacattgaga aaagaaaaga agcattagtt gtttataggg aagattatga
    11821 aaaagaaatt ggcgaaaaaa ctcgattagg ttttcatatt aacccttata attatgatag
    11881 tgctgatgag aaaaatttat ttggctattt acaaaagtat ttagaggaaa acgaggcaat
    11941 cattgatatt tattttacgg gcggattaac agaccaaacc cataatgatt tttattttga
    12001 atactacagt ccagaaaaag aaaggattgc ccgctatttt cccgattttc ttatagaaac
    12061 taataagggg aggtatttgg tgatagaagt taaatcaaac agagaacgaa ttacttatga
    12121 agagaataaa cagcaatatc ttgctggtaa aaagaaaatt tttgatgaag tgttcgccaa
    12181 agaaatcggc tttagagagt tccaacaagc caataaaaat tttgaatatc gcattatttt
    12241 tgacgcttca tttcgtaaac accaacagga gttgttggaa agtataagaa aaagtataag
    12301 agaaatttga tataatttta ttgaaatagt gagtagcagg gtatggtaaa ataagatgaa
    12361 ataaaaacta tgaaagaaaa aattaagaaa attttaaaat atggttgata aaaatagaag
    12421 tttatccgct tttgtaaaag aatttgcaaa atattcaact agtgctgaac gaagcaattt
    12481 cttgtttgag tgtagacggg aataaaaagt tggatggtta tataataagt atgttgagaa
    12541 taactcaacg ccactccagt ccagtggtaa aataaaaaat ttttataata tgtca
//
LOCUS       DAOTQD010000013        11430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_5361_length_11430_cov_7.820835, whole genome shotgun sequence.
ACCESSION   DAOTQD010000013 DAOTQD010000000
VERSION     DAOTQD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_11430_cov_7.820835"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..114)
                     /locus_tag="P5570_01050"
     tRNA            complement(32..114)
                     /locus_tag="P5570_01050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Leu,seq:taa)
     gene            complement(122..194)
                     /locus_tag="P5570_01055"
     tRNA            complement(122..194)
                     /locus_tag="P5570_01055"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(159..161),aa:Cys,seq:gca)
     gene            complement(284..1255)
                     /locus_tag="P5570_01060"
     CDS             complement(284..1255)
                     /locus_tag="P5570_01060"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRR39331.1"
                     /translation="MKIDNKKFNRIKKEIQEAKRILILSHRAPDGDAVGSSLALHTFL
                     KRQKKKSVVFISQPPQFLKFLPGFDEIQRKALFKNDNFDLIFALDYANKERLEKPQDF
                     QLLSRKTITIDHHISGKRVGKIKIVDSKASSTTEVLYYFFKFQKTKIDKKLATLLLTG
                     ILTDTVGLSQSGQNHRTVEKIVGELAIAGGDLFKIMAAYQNFDFKRAKILAKFLERAE
                     SDEKLNLIYSYLLKSDFNGEKGLNLSEPPMFPDFLSSIGEAKVYITLIEQKDRIKVSL
                     RSRNGINVAKIAKKFGGGGHKYAAGCKIKGDIFEVLKKIKKELKKNF"
     gene            complement(1264..2727)
                     /locus_tag="P5570_01065"
     CDS             complement(1264..2727)
                     /locus_tag="P5570_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRR39332.1"
                     /translation="MFKIPVVVVLGHIDHGKSSLLEAIKKEFRITKKESGGITQHMGA
                     YEVETQGKKITFIDTPGHEAFSGMRARGASVADIAILVIAADDGVKPQTREAISAIKK
                     AEIPMVVCLNKMDKPTADPQRVKSQLQKENVLVEDFGGDVPVVEVSAKTGKGINDLLE
                     IINLLWEMSAEKERKKEDTATKGFVIESYLDSLRGPTATLILESGELKIGDIVGSSST
                     VGKIKTLEDFQGNPKKIVFPGEPAVITGIEEVPGLGEIFKKFNSIEEAQSHLEKKENL
                     SFEAAPTKEGLNIILKTDARGTLEVIEKILKDLEKEGEKVNIIKGEVGEVNFSDIKLS
                     EDLDAQIISFRSKVPLQVKNLAQQRNINILSFEIVYDLIDNVRKLVDQKKKKAEKKRE
                     EAGRVKITDCFKTQSKKGKYQQIIGGQVIDGAIKKGEVEIERQEKIIGKGRVLEIQKE
                     RKKIEKGDVGQQIGISYEGNVKVKEGDILIAYQYVVS"
     gene            complement(2859..3338)
                     /locus_tag="P5570_01070"
     CDS             complement(2859..3338)
                     /locus_tag="P5570_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HRR39333.1"
                     /translation="MFDFISQNIDIILIVIVAVFLIWNILLEIRLKKERQRVNSFFKG
                     KKTEDLEGVISEILRRERKTEIDLQKIFKKLKTLDEIALKSIQKVGIVRFNPFEGIGG
                     NQSFSIALLDQKDAGVVISSYHSKDTTRVYAKPIKNGESEYPLSKEEEEAIKRAIGS"
     gene            complement(3647..3958)
                     /locus_tag="P5570_01075"
     CDS             complement(3647..3958)
                     /locus_tag="P5570_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HRR39334.1"
                     /translation="MQLPEKKFQKRVENFTCDKCGFFVKGTGYTDHCPNCLWSKHVDI
                     NPGDRKAKCGGLMEPVGLGKKGDKYIIYYYCQRCGYKFQVKVASSDNFKEILKLNSKK
                     I"
     gene            complement(4075..4638)
                     /locus_tag="P5570_01080"
     CDS             complement(4075..4638)
                     /locus_tag="P5570_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRR39335.1"
                     /translation="MKDPVTVSDPNPVKKGSKTEENLWKAFAGEAQARNKYTYFAKIA
                     REAGFEAIAAVFEETAKNELEHAKMIWKLLGQWSSTKVNLKKAIEGEHYEWSQMYPEF
                     EKIAKEEGFKDAATFFKEVAEIEEQHEKRYQRLLDDIEKNEVFKKKKKVMWVCRNCGY
                     VHFGTEPPKECPNCRHPRSYFEVRCQY"
     gene            complement(4687..4965)
                     /gene="nrdD"
                     /locus_tag="P5570_01085"
     CDS             complement(4687..4965)
                     /gene="nrdD"
                     /locus_tag="P5570_01085"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRR39336.1"
                     /translation="MVTKTSSKIFCHDCKKELKIDKDKKIENGVRLTYDKNGEKIEIF
                     KCKNCYLEDKSLKNYQPCEVYSRIVGYLRPVQQWNEGKKQEFKERKEF"
     gene            complement(4982..5230)
                     /gene="atpC"
                     /locus_tag="P5570_01090"
     CDS             complement(4982..5230)
                     /gene="atpC"
                     /locus_tag="P5570_01090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRR39337.1"
                     /translation="MLVNILSLNKILYQGEAESLTVPGAKGTLTVLPHHAPLITFLEK
                     GKLKLKKEKELFFEIEKGILEVTPSEVNVLVSITEHSY"
     gene            complement(5315..6658)
                     /gene="atpD"
                     /locus_tag="P5570_01095"
     CDS             complement(5315..6658)
                     /gene="atpD"
                     /locus_tag="P5570_01095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRR39338.1"
                     /translation="MAKIIQVLGPVVDVEFEEKVPKIFNALKVERVNLTLEVEQNLGE
                     KRVRCLAMGPTEGLKRGDEVIDTGSPIKVPVGDKSLGRVLNVLGNPIDGKGEIEAKDL
                     WSIHRKAPKMVEQETKIEILETGLKAIDLLAPIPKGGKAGLFGGAGVGKTVLIQELIR
                     NVAEVHKGYSVFTGIGERSREGNDILLEMKNAGVLKNCALIFGQMNEAPGVRFRVPSA
                     GLTIAEYFRDVEKKDILFFADNIFRFALAGSEVSALLGRIPSQTGYQPTLFQEISELE
                     ERITSTKEGSITSIQAVYVPADDLTDPGVVSVFSHLDSSIVLSREIADLGIYPAVDPL
                     ESSSQILDPSIVGEDHYFVAMEVKRVLKKYQDLKDIISILGMEELSDEDRLTVQRARK
                     IQRFLSQPLHVAEIFTGKKGEYVSLKDTIDGFKKIISGEVDSIKEEDLYMKGKLV"
     gene            complement(6878..7795)
                     /gene="atpG"
                     /locus_tag="P5570_01100"
     CDS             complement(6878..7795)
                     /gene="atpG"
                     /locus_tag="P5570_01100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRR39339.1"
                     /translation="MKLKEVKNKINATQNIYGLTQAMELIAAMKVRKLKNATLNSRPF
                     VRKSIELLIKLIEYQKSLQEDVQNNLQDDKKNNPSVLTQKILAVVFASDKGFCGVYNK
                     NILRYAQRQIEQLKKEGEVELITIGKKTTNFFKTKKYTIKMEFPDIKEFEEKKRTDEL
                     FKYLEDFYQKGAYKKILLFATHYFTSFVQSPRTVQVFPADEKKLREMMKETAGKTVSE
                     KKIDYIFEPSFEEIFDEALSQLMAAELYHAVLEARTSEEASRMMAMKRAMDNAKEVIK
                     NLTLEYNKARQAQITSEVSEITSAKEAIE"
     gene            complement(7770..9287)
                     /gene="atpA"
                     /locus_tag="P5570_01105"
     CDS             complement(7770..9287)
                     /gene="atpA"
                     /locus_tag="P5570_01105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRR39340.1"
                     /translation="MDTFEILKKEIENLELKPGKEEIGEVVEVKDGIAMIAGLKEVES
                     FEMIKFETADVLGVALNLGEDIVGAIVLGDFSKIREGDIVKRERKTLSLPVGENLIGR
                     VIDPLGNPLDGKGEIQGTKLAPLEKIGPSVVEREPVNEPLHTGIKMIDALIPIGRGQR
                     ELFLGDRTTDKSNHALTIILNQKNEAKRPICIYCPIGKKEAEIARTVELLEKYGAFEY
                     TIVVAASASLPASLWYLAPYAACAQGEYFMEKGQDAVVIYDDLTKHGYAWRQIALILR
                     RPPGREAYPGDIFYLHSRLLERAAKMHKDKGGGSLTAIPILETQAGDITGYIPTNVIS
                     ICDGQIYFDTSLYLKGQKPEVNVGLSVSRVGSAAQIKAMKKTASTLKLELAQFTELEQ
                     FVEFIEEVDEDTKKSLKRGERIREILKQEKLKPLPIEKEIVSIYASTKGFLDNVRIED
                     VKKIENELYEVIEFKKPDIFKSIREKKELDDTTQAALDKILEDVTKKYEVEGSKK"
     gene            complement(9266..9652)
                     /locus_tag="P5570_01110"
     CDS             complement(9266..9652)
                     /locus_tag="P5570_01110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HRR39341.1"
                     /translation="MIKKLVKILLSLTQGKSDKEKQEIVRVFLKLLAEKNQLHLLPAI
                     LKELKREEKKQKIELILARNFEQEFLQKIERGVKKTLGEKEIKLRIEPEIIGGFLAKD
                     ENYLIDGSIKGILNNFKERLWTHLKF"
     gene            complement(9645..10142)
                     /gene="atpF"
                     /locus_tag="P5570_01115"
     CDS             complement(9645..10142)
                     /gene="atpF"
                     /locus_tag="P5570_01115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRR39342.1"
                     /translation="MNPLDTFGINWKILIGQIINLIILLYLLQRFAFKPFLKILSERR
                     EKIEKGVKLAEESKEKIKLAEEERAKILKEGEEKAKQILKESEERGKQKEKEILTAAE
                     VQRVKILEQAQQMAQKEIEKRKREFSQKNLELVLAVSEKILKEKINSEKDKQIIENLL
                     TSNND"
     gene            complement(10212..10439)
                     /gene="atpE"
                     /locus_tag="P5570_01120"
     CDS             complement(10212..10439)
                     /gene="atpE"
                     /locus_tag="P5570_01120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRR39343.1"
                     /translation="METEAIKDLAIALTIAIGAIAPAISIGKIGSKGLEAMGRNPEAA
                     SKIQTGMILSIAFVEAIAIYCLVVAIILKFV"
     gene            complement(10484..10738)
                     /locus_tag="P5570_01125"
     CDS             complement(10484..10738)
                     /locus_tag="P5570_01125"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRR39344.1"
                     /translation="MSSPVKNQNKDKSNNFFYAVSLGLEMGFLIALPLVIFLILGVFL
                     DKKFETFPFYLISAMILGLVLTFLDVYYFVLPFLTKKLKK"
     gene            complement(10704..>11430)
                     /gene="atpB"
                     /locus_tag="P5570_01130"
     CDS             complement(10704..>11430)
                     /gene="atpB"
                     /locus_tag="P5570_01130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRR39345.1"
                     /translation="DNFNFMDVPEISIAAEKLFDVFGFPITNTFLLTLIIGLCVVLLS
                     FFAFKKKKIIPDSLQNFFEWAFEALLGYVDSITNDRKKTLKIFPLTAVIFIIVLLSNL
                     LEILPGIGVFNFLRSPSSDLNFTLALAVFSMVSVHILSLQDLGFSNYIKKYISKNPIF
                     LFVGLLEGMGELTRTFSLAIRLFGNLLAGEVLLMIVSFMLAYVFPLPFLALEIFVGFI
                     QALIFSSLIIIFYVVTSEKPEQR"
BASE COUNT         3482 a         2243 c         1444 g         4261 t
ORIGIN      
        1 aacttacaaa cattattatt tttatcaaga gtgccgaggg cgggagtcga acccgcacga
       61 ccttgcggtc aagggatttt aagtccctcg tgtcttcctt tccaccacct cggcaggata
      121 aaggcacggg tgggaattga acccacgaat aacagttttg cagactgtcg ccttagccac
      181 ttggctaccg tgccataatt attttttaga aaaaatggtt ttacttttat ttatatttta
      241 acgccttttt tatctttttt caattatttt tttcaaaagc ccattaaaaa tttttcttta
      301 attctttctt gatttttttt aatacctcaa aaatatctcc ttttatttta caaccagcag
      361 catatttatg acctcctccg ccaaattttt tagcgatttt agcaacattt attccgttgc
      421 gtgaacgaag agaaaccttt atcctgtcct tttgttctat taaagtaata taaacctttg
      481 cctcgccaat actagaaaga aaatcaggaa acattggtgg ctcagataaa ttaagtcctt
      541 tttctccatt aaaatcagat tttaaaagat aagaataaat taaattcagt ttttcatcac
      601 tttctgccct ttcaagaaat tttgctaaga tctttgcccg tttaaaatca aaattctggt
      661 aagcagccat tattttaaaa agatctccgc cagcgattgc taattctcca acaatttttt
      721 caactgttct atgattttga ccactttgag aaaggcctac tgtatcagtt aaaattcctg
      781 ttaaaagaag agtggctaac tttttatcaa tttttgtttt ttgaaattta aaaaaataat
      841 agagaacctc agtagttgaa gatgctttcg aatctactat cttaattttc cctactcttt
      901 taccactaat atgatgatca atagtgatag tttttcgaga aagaagttga aaatcctgcg
      961 gtttctctaa tcgttcttta tttgcataat caagagcaaa aattaaatca aaattatcat
     1021 ttttaaacaa ggcttttcgt tgaatttcat caaatcctgg taaaaatttt aaaaattgag
     1081 gtggttgaga tataaaaacc actgacttct ttttttgtct tttaagaaaa gtatgtagtg
     1141 ccaaggatga acccactgcg tctccatcgg gcgctcgatg gctcaatatt aaaattcttt
     1201 ttgcttcttg aatttctttt ttaatccgat taaatttttt attgtctatt ttcacaaaat
     1261 taattaagaa accacatatt gataggcaat caaaatatct ccttctttaa cttttacatt
     1321 tccctcataa gaaataccaa tttgttgtcc cacatctcct ttttctattt tttttctttc
     1381 tttttgaatt tctaaaaccc tgccctttcc tatgattttt tcttgtcttt ctatttccac
     1441 ttctcccttt ttaattgcac catcaattac ctgaccacca ataatttgtt gatacttgcc
     1501 ttttttgctt tgagttttaa aacaatctgt aattttcact cttcctgcct cttctctctt
     1561 tttttctgct tttttctttt tttgatctac tagtttacga acattgtcaa taagatcata
     1621 aacaatctca aaacttaaaa tattaatatt cctttgttgg gctaaatttt ttacttgcag
     1681 aggcaccttg gatcgaaaac ttattatttg tgcatctagg tcttctgata gttttatatc
     1741 agaaaaattt acttctccta cttctccctt tataatattt actttttctc cttctttttc
     1801 aaggtctttt aaaattttct cgatcacctc caatgtcccc ctagcgtcag tttttaaaat
     1861 gatatttaat ccttcttttg ttggtgctgc ttcaaaactt aaattttctt ttttttcaag
     1921 atgggattgc gcttcttcaa ttgaattaaa ttttttaaaa atctccccca gtcctggcac
     1981 ttcctcaatc cctgtaatta cagctggctc tcctggaaaa actatttttt taggattgcc
     2041 ttgaaaatct tctaatgttt ttattttacc aacagttgag gaagatccta ctatatctcc
     2101 tatttttaat tctccacttt ctaaaattaa cgtggcagta ggacctctta aggaatcaag
     2161 ataactttct attacaaatc cttttgttgc cgtgtcctct ttttttcttt ctttttctgc
     2221 tgacatttcc cataaaagat ttattatttc taaaagatca ttaatgccct taccagtttt
     2281 tgctgaaact tcaactactg gcacgtctcc tccaaagtcc tcaactaaaa cattctcctt
     2341 ttgaagttgg ctttttaccc gttgaggatc agcagtgggt ttgtccattt tattcaaaca
     2401 aacaaccatc ggaatttcag cttttttgat cgctgaaatc gcctctcgag tttgaggttt
     2461 tactccatca tcagccgcta tcaccaaaat tgcaatgtca gccacgcttg ctcctcttgc
     2521 tctcattcca gaaaaagcct cgtggccagg agtatcaata aaggtaattt ttttaccttg
     2581 ggtttcaacc tcataagcac ccatatgttg agtgatgcca cctgattctt ttttagtaat
     2641 tctaaattcc ttcttgatcg cttcaagaag acttgatttt ccatggtcaa tatgacccag
     2701 gactacaaca acaggaatct taaacataaa aaattcaatt tctaatttct aatttctaaa
     2761 taaatttcaa tgaccaaaat ttaaaattaa aaatagtttt gaattttaaa cattagaatt
     2821 tagaatttgt ttagagtttg gaatttggaa tttggaattt acgagcctat ggctcgtttt
     2881 attgcttctt cttcttcttt tgaaagtgga tattctgact caccattttt aattggtttt
     2941 gcataaaccc gcgtagtatc ctttgaatgg tatgaagaaa ttactacacc agcgtctttt
     3001 tgatcaagta aagctattga aaaactctga ttaccaccaa ttccttcaaa aggattaaag
     3061 cgaactattc ctactttttg aatggatttc aaagcaattt catctaatgt tttaagtttt
     3121 ttaaaaatct tttgaagatc tatttctgtt tttctttctc ttcttaaaat ttcactaatg
     3181 acaccttcta aatcttctgt ctttttacct ttaaaaaaag agttgactcg ctgacgctct
     3241 ttttttaatc ttatttccaa aagaatattc caaatcaaaa aaactgcaac gatcacaatt
     3301 aaaataatat caatattttg agaaataaaa tcaaacataa ttataaatta gaaattttta
     3361 ttgatccttg cgaggagtct cgcctttggc gagacgacga agcaatttcg agcgaatact
     3421 ctgggattgc cacggtcgcc cgaaggcgac ctcgcaatga caccttcttt tcaacgctcg
     3481 tccatttacg ctgcggctcg cgtcaaagac agcgggcgag cattgaaaaa ggtttgaaaa
     3541 ttaaatcatt agtcatttaa tgaaaattag aaattagaaa tttttaaaag tatcttttat
     3601 tctattaaat tttcatcttt tttgcaagca aatagataat ggatttttaa atctttttag
     3661 aattcaattt taatatttcc ttaaagttgt cactggacgc aactttcact tgaaatttat
     3721 aaccgcatct ttggcaataa taataaataa tatatttgtc tccttttttt cctaagccga
     3781 caggttccat taatccacca cattttgctt ttctatctcc tggatttata tctacatgct
     3841 ttgaccaaag gcaatttgga caatgatcag tatatcctgt tccttttaca aaaaaaccgc
     3901 atttatcaca agtaaaattt tcaactcttt tttgaaattt cttttcaggc aattgcataa
     3961 taataaattt tgacgctttt taaatatagt attagatgct aaataaaaat taatattaat
     4021 atcaattata taaaaaacag ggttgagccc tgttttttat attttttcat ttctttaata
     4081 ttggcatctt acttcaaaat aactccgcgg atgacgacag ttagggcatt cttttggtgg
     4141 ttctgtgcca aaatgaacat atccacaatt gcgacaaacc cacattactt tctttttctt
     4201 tttaaaaacc tcattctttt ctatatcgtc taataatctt tgatatcttt tctcatgttg
     4261 ttcttctatt tcagctacct ctttaaaaaa tgttgctgcg tctttaaatc cttcttcttt
     4321 tgctattttc tcaaactcag gatacatttg agaccattca taatgttctc cctcaattgc
     4381 ctttttcaga tttacctttg tgcttgacca ttgacccaat aatttccaaa tcattttagc
     4441 atgttctaat tcgtttttag cagtttcttc aaacacagca gcaatagctt caaatcctgc
     4501 ctcacgggca atttttgcaa aatatgtata tttgtttcta gcttgggctt caccagcaaa
     4561 agccttccac aaattttctt cggtctttga tccttttttt actggattag gatcagaaac
     4621 agtaaccgga tctttcatat aattattttt tttaactctt tttatttttt tcgaccttta
     4681 ttatatttaa aattcttttc tctctttaaa ttcctgtttt ttgccctcgt tccattgctg
     4741 aaccggtctt aaataaccca cgattcgaga ataaacctcg cagggctggt aatttttcaa
     4801 tgatttatct tcaagataac aatttttaca tttgaaaatt tcaatttttt ctccgttttt
     4861 atcataagtt aatcttacac cattttcaat ctttttatct ttgtcaatct ttaattcttt
     4921 tttacaatca tggcaaaaaa tttttgatga agtttttgta accataaata aaaataattt
     4981 actaataaga atgttcagtt atgcttacta atacatttac ctcacttgga gtaacttcta
     5041 aaattccctt ttcaatttca aaaaataatt ctttttcttt tttcaatttt aattttccct
     5101 tttctaaaaa agtaattaaa ggagcgtgat gaggtaaaac ggtcaaggtg ccctttgctc
     5161 caggaacagt aagcgattcg gcttcgccct gataaagtat tttattcaaa gaaagaatat
     5221 taactaacat aaattagaat ttacaaattt ttaattttta aaacagtaag aaagtctgta
     5281 ataattgtaa attagtaaat ctgtaaattg aaaattaaac aagctttcct ttcatataaa
     5341 gatcttcttc ttttattgag tcaacttcac cagaaataat ttttttaaaa ccatcaattg
     5401 tatcttttaa agaaacatac tctcctttct tccctgtaaa aatttcagcc acatgaaggg
     5461 gttgagataa aaacctttga atttttcgtg ctctctgaac tgttaatcga tcctcatccg
     5521 aaagctcctc catccccaaa atagaaataa tatctttcag gtcttgatat ttctttaaaa
     5581 ctctttttac ctccatggcc acaaaataat gatcttcacc aacaatgctt gggtctagga
     5641 tttgagagct actctctaga ggatcaacag ccggataaat tccaagatcc gcaatctctc
     5701 ttgataaaac aatggaagaa tcaagatgag aaaatactga tactacacca ggatctgtta
     5761 aatcatcagc cggtacataa actgcttgaa ttgaagtaat tgatccttct ttggttgaag
     5821 taattctctc ttccaattct gatatttctt gaaacaaagt tggttgataa ccggtttgag
     5881 aaggaattct tcccaacaac gccgacacct cagatcctgc taaggcaaat ctaaaaatat
     5941 tatcagcaaa gaaaagaata tctttcttct caacatcacg aaaatattca gcaatggtta
     6001 atccagctga aggcactcta aatcttacac ctggtgcctc attcatttgt ccaaatatta
     6061 aagcacaatt tttaagcacc ccagcatttt tcatttcaag taaaatatcg tttccttccc
     6121 ttgatctctc tccaatgcca gtaaaaactg aatagccctt atgaacttca gccacatttc
     6181 gaattaattc ttgaatcaac accgtttttc caacaccagc gccaccaaat aatcctgcct
     6241 tgcctccttt gggaattggc gctaacaaat caatggcttt taatcctgtt tccaaaattt
     6301 caattttagt ttcctgctca accatttttg gagcttttcg atgaatagac caaaggtctt
     6361 ttgcttcaat ttctccttta ccatcaatag ggttacccaa cacatttaat accctgccca
     6421 gcgatttatc acctacaggc actttaatag gagaccctgt atcaattact tcatctcctc
     6481 tttttaatcc ttctgtggga cccattgcta aacatcttac ccttttttct cctaaatttt
     6541 gctccacttc taaagttaaa ttaacccttt ctacttttaa agcgttaaat attttgggta
     6601 ctttttcttc aaattctaca tcaacgacag gacctaaaac ttgtataatt tttgccatag
     6661 tagtaaattg cgtcaattgt taaaattgtt aagcggtagc gtaacgcgca acgtcaagcg
     6721 tgtaaacttt gcactttccc gctgtaagac gcatttcttt caatgtattc cttcgattcg
     6781 actcaaaaaa taattatttc ttggtaaacc cgccgtgggc ggacgtatcg aaggaccatc
     6841 ttaattaaat tagtaatgta atgtaatttg taattaatta ttctattgct tcctttgctg
     6901 aagtaatttc agaaacttca gaagtaattt gggcttgcct tgctttatta tattctaatg
     6961 tcaaattttt tattacttct tttgcattgt ccattgctct tttcattgcc atcattcgac
     7021 tcgcttcttc tgatgttcgt gcttctaaaa cagcatgata taattctgca gccattaatt
     7081 gagaaagcgc ttcatcaaaa atttcctcaa acgatggctc aaatatgtaa tctatctttt
     7141 tttcagaaac agttttacct gcagtttctt tcatcatctc tcttaatttt ttttcatcag
     7201 caggaaaaac ttgaactgtt cttggacttt gcacaaaaga agtaaaataa tgggtagcaa
     7261 aaagaaggat ttttttgtac gctccctttt gataaaaatc ttctagatat ttaaataatt
     7321 catcagtcct ttttttctct tcaaattctt taatgtctgg aaattccatc ttaattgtgt
     7381 atttttttgt tttaaaaaaa ttagttgttt tttttccaat agtaattaat tctacctctc
     7441 cttccttttt caattgttca atttgccgtt gagcatacct taaaatgttt ttattgtaaa
     7501 ctccacaaaa ccctttatca gaggcaaaaa caacagctaa aattttttgt gttaggactg
     7561 aaggattgtt ttttttatca tcttgcaaat tattttgcac atcctcttgc aaactttttt
     7621 gatactctat taattttatt aaaagctcta ttgattttct tacaaaagga cgagaattca
     7681 aggttgcgtt ttttaatttc ctcactttca ttgccgcaat caactccatc gcctgggtca
     7741 aaccataaat gttctgtgtg gcatttattt tattttttac ttccttcaac ttcatatttt
     7801 tttgttacat cttcaagtat cttatctaat gcagcttgag tagtatcatc taattctttc
     7861 ttctctctaa ttgatttgaa aatatcaggt tttttaaact caattacttc atataattca
     7921 ttttcaatct tcttaacatc ctcgatccgc acattgtcta aaaatccttt tgtactagca
     7981 tatatcgata cgatctcttt ttcaatgggc aatggtttca acttttcttg ttttaatatt
     8041 tctcttattc gttcaccccg ttttaaactt ttttttgtat cttcatcaac ctcttcaata
     8101 aattctacaa attgttctag ttcagtaaac tgagccagtt ccaattttag agtagaagcg
     8161 gtttttttca ttgctttgat ttgagcagct gatcctaccc ttgaaactga taaaccaaca
     8221 tttacttctg gtttttgacc cttcaaataa agcgatgtat caaaataaat ctgaccatca
     8281 caaatagaaa tcacattagt aggaatatat ccagtaatat caccggcttg ggtctctaga
     8341 atcggaattg cagttaaaga accaccccct ttatccttgt gcatttttgc tgctctttcc
     8401 aacaaccttg aatgaagata aaaaatatct ccaggatatg cctctctgcc tggcggtctt
     8461 cttaaaatca aagcaatttg tctccaagca taaccatgtt ttgttaaatc atcgtaaatc
     8521 acaacagcat cttgaccttt ttccataaaa tattcaccct gagcgcaagc agcatatgga
     8581 gcaagatacc ataatgaagc gggcaatgaa gcggacgcgg caacaactat tgtatactca
     8641 aaagcaccat atttttctaa taattcaact gttcttgcaa tttctgcttc ttttttgcca
     8701 ataggacaat aaatacaaat aggccttttt gcttcatttt tttgatttaa aataatggtt
     8761 agggcatgat ttgatttatc agtggttctg tctcctaaaa acaattctct ttgtcctctt
     8821 ccaataggga tcaaagcatc aatcatttta atacctgtat gcaggggctc atttacgggt
     8881 tctctttcta ccaccgaagg gccaattttt tccaagggag ccaattttgt tccctgaatt
     8941 tctcctttac catctaaggg attgcccaag ggatcaataa ctcttccaat taaattttct
     9001 cccacaggca aagaaagagt ttttctttct cttttaacaa tatcgccctc tctaatttta
     9061 gaaaaatcac ccagtacaat agcacctact atatcttctc ccaaattaag tgcaacacct
     9121 aaaacatctg ctgtttcaaa ttttatcatt tcaaaacttt caacttcttt taatcccgca
     9181 atcatagcaa taccatcttt tacttctacc acctcgccta tttcttcttt tcctggtttt
     9241 aattctaggt tttcaatttc tttttttaaa atttcaaatg tgtccataat ctttccttaa
     9301 aattatttaa aatacccttg attgaaccat caatgagata attttcgtct tttgctaaaa
     9361 atccaccaat aatctcaggt tcaatcctaa gtttaatttc tttttctcct aatgtttttt
     9421 tgacacctct ttctattttc tgtaaaaatt cttgctcaaa atttcttgcc agtatcaact
     9481 caatcttttg ctttttttct tctcttttta attcttttaa aatagccggc aaaagatgca
     9541 gttggttttt ttctgctaat agttttaaaa acacccttac aatctcttgc ttttctttgt
     9601 cgcttttacc ttgagttaat gaaagtaata tcttaactaa tttcttaatc attgttgctt
     9661 gttaaaagat tctcgataat ttgcttatct ttttcagaat taatcttttc ctttaaaatt
     9721 ttttcgctca cagccaatac taattcaagg tttttttggg aaaactctct tttgcgtttt
     9781 tcaatctctt tttgagccat ttgctgggcc tgttctaaaa tctttactct ctgtacttct
     9841 gccgcggtta aaatttcttt ttctttttgt ttccctcgct cctcactttc ttttaagatt
     9901 tgctttgctt tttcttctcc ttcttttaaa atcttggccc tttcctcttc agccagtttt
     9961 atcttttctt ttgattcctc agctaattta acgcctttct caatcttttc tcgcctttct
    10021 gagagtatct tcaaaaatgg tttaaaagca aaccgctgaa gcaaataaag cagtattatc
    10081 aagttgataa tctgaccaat taaaatcttc caattaattc cgaaggtgtc caatggattc
    10141 attgaaaaaa tttaatcaaa acggtaaaga tgtttttttc atcattacca attttgataa
    10201 taattaactt attaaacgaa ctttaaaatt attgccacta ccaaacaata aatggcaatt
    10261 gcctcaacaa aagcaataga cagaatcatt cctgtctgaa tttttgaagc ggcttctggg
    10321 tttctaccca ttgcttcaag tccctttgat cctattttgc cgatacttat tgctggggct
    10381 attgctccaa tagctattgt taaagcaatg gctaaatctt ttatagcttc tgtttccata
    10441 ttttttaaaa aaattaacta taatttctct cgacctttaa tatttatttt ttcaactttt
    10501 ttgttaaaaa cggtaataca aaataataaa catcaaggaa ggttaaaacc aatcctaata
    10561 tcatagcaga aattaaataa aaaggaaagg tctcaaactt tttatcaaga aatacaccca
    10621 atatcaaaaa aatcactaag ggtaatgcaa ttaaaaaacc catttccagt cccaatgaaa
    10681 cagcataaaa aaaattattt gatttatctt tgttctggtt tttcactggt gacgacataa
    10741 aaaataataa ttaaagaaga aaaaataagg gcttgaatga acccaacaaa aatctccaat
    10801 gccaaaaagg gcaaaggaaa cacataagcc agcataaatg aaacaatcat taataacacc
    10861 tcgcccgcca aaagattccc aaaaagccga attgccaaag agaatgtccg cgtcaactcg
    10921 cccattcctt ctaaaagtcc aacaaaaaga aaaatgggat ttttactaat atatttttta
    10981 atataattag aaaatcccaa gtcctggaga gataaaatat gaactgaaac cattgaaaaa
    11041 acagccaaag ccaatgtaaa atttaaatca gagctaggag atcttaaaaa attaaaaacg
    11101 ccgatgcccg gcaaaatttc taaaagatta gacaaaagaa caataataaa aataacagca
    11161 gtcaagggaa aaattttcaa tgtttttttt ctatcattgg taatactatc cacatatccc
    11221 aataatgctt caaaagccca ctcaaaaaaa ttttgcaaag aatcgggaat aatcttcttt
    11281 tttttaaaag caaaaaaaga taatagaacc acacacaacc ctattattag agttaataaa
    11341 aaagtattag ttataggaaa gccaaaaaca tcaaataatt tttctgcagc aattgaaatt
    11401 tctggaacat ccataaaatt aaaattatct
//
LOCUS       DAOTQD010000014        11008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_5599_length_11008_cov_8.241486, whole genome shotgun sequence.
ACCESSION   DAOTQD010000014 DAOTQD010000000
VERSION     DAOTQD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11008
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5599_length_11008_cov_8.241486"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..486
                     /locus_tag="P5570_01135"
     CDS             175..486
                     /locus_tag="P5570_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39346.1"
                     /translation="MSPFERFKKSNTEGNLWIYILALGKNEVVPDENVKKLIFEKFGF
                     LPDSFLVNRVLYRLRQGKYIETERFKGKKAYKTTEDGLRELEKMKVFCQDLLQKILKQ
                     G"
     gene            complement(487..1101)
                     /locus_tag="P5570_01140"
     CDS             complement(487..1101)
                     /locus_tag="P5570_01140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRR39347.1"
                     /translation="MLDLKRDLFKGLEKKVGRIFSYLPLSPNQWTLLSVFFALISLYF
                     LINQNLILTIVFFIFAGFLDFVDGAVARYKNISTKVGAYLDTVCDRYVEGILLFGMLF
                     LPLPEILFPSYIWIFLILFGSMMTTYAKATAKEKDLVNQELKGGILSRGERIILLIIS
                     LIIGIFNFTFMVYVLIVVAILANFTALQRIHSAIQLQKNSSSIN"
     gene            complement(1159..1917)
                     /locus_tag="P5570_01145"
     CDS             complement(1159..1917)
                     /locus_tag="P5570_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRR39348.1"
                     /translation="MDIFQAVILGIVQGLTEFLPISSSGHLVIFQKLFGFTQAPIAID
                     TLVHFGTLVSLIFFFWSDIKKVLKDKKLILLLIIGTIPVVIVGFLLQEKIEAIFNSLL
                     LVGISFLITATILFFTRFVKNSQKNIKEIGAKDAIIVGLFQALSILPGVSRSGSTISG
                     SLFCSIKKEDAFNFSFYLGMIAILGATILQIPEILNFNADEAIGGFVGFFFAAVVGFF
                     ALKGLKKVVINGKLYYFGIYCAIIGVICIISQYL"
     gene            complement(1923..2519)
                     /gene="plsY"
                     /locus_tag="P5570_01150"
     CDS             complement(1923..2519)
                     /gene="plsY"
                     /locus_tag="P5570_01150"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRR39349.1"
                     /translation="MIITFILLLIFAYLLGSIPSGYLISKAKGVDIRKVGSGNIGGAN
                     VSRAFGWRWGLVVSICDILKGVIPTFLAVKFLDSQWQVAAVAIFAVLGHLFPVWLSFK
                     GGKGVATTFGILIVLFGWKVFLVWIFIWILLLLIFKITSVTNLLMIAFVPLIFITIFD
                     SLPYFVLGLVLVGLIWWAHRQNLKRIIDGKELKMNFQY"
     gene            complement(2568..2942)
                     /locus_tag="P5570_01155"
     CDS             complement(2568..2942)
                     /locus_tag="P5570_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRR39350.1"
                     /translation="MDKSLLKELKEKLEEERIELERILSSFSKKDRKKTSDWETKFPQ
                     FGSHTSEQDENADEVEEYTNLLPLEYRLELQLLDIERAIEKIKKNKNYGICERCGKEI
                     SKERLMVIPETKFCSKCGARTS"
     assembly_gap    3087..3186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3208..7731)
                     /locus_tag="P5570_01160"
     CDS             complement(3208..7731)
                     /locus_tag="P5570_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39351.1"
                     /translation="MALFKKNKKDIKSSKGYLDKVRAEQKKRSEDELHLLEIRTMAKD
                     LARFSIPEEKLSQLQKQKQKQEQKEKEKALLALKKKREEELKKQAIEEKKMAALELQR
                     KAEREKAEREKEEKIKAEKEKMAALKEESKEEEERRKEEFRQGLLKKKKSEEEKREEE
                     KKLILKIKEQKEKISQKKENKKELIKINKQLDDEIKKIESLFQSKEKEVAKIAKIHRQ
                     YVPELSGEKTKEQYNALKAKKEEMEKDIAKIQDIKKIIENEINFSFSKNIEEEEEEEE
                     IKIVNESQSRWESDLKQLVTEEEKLNKELEDLRSQKTEKEQKIAEMKNKIQEKDAKKK
                     ELQEEINNFKKSQGFLKRIIPGNVSKKEEKTIKKEEVLSLQEELERLKENTNQQMLEI
                     KRMIEMLRKEKERLMAQKEEKEQQIKEKEKELKFETERAQANTQQNEDLLNKENIEKR
                     EEVEKEINFKKTEKEELKKAIEKIDKEVENVREELKKLEEEKQTLDDEIKPFLIEKEK
                     EEQKAQKRQKAQKRIEQLSLILENEIQKTRAIKDKTQEKLEQLNEKIKELQQREEEIE
                     SQEQEIEDQIKRLGGNRELEEKRWKVEEQRKEIKKERWQIEDEQKKTKEELKLIEEKY
                     NALMSFNDQKTRNQKIRDQIKEKEEEIEKEWQEIKNDVKDVFAGKKLAPVEEEKVEKE
                     FISETSLFSQEEKSDSKDDQLIIELEKIKKEKEELLGALEEIKRFQTEEDEESIDSLK
                     SIEEEIARLEKRIEEEEEEEEEELKIDDVIRNAQILLEEVRSLKSEIFQNQKKLKEFE
                     EKIIQEKQKEEIKKENKEAIQERKLLEEEKENILKELKNLSEEKKQKELLLKNLISQL
                     DNLRNKTQETKLEIERFKKEKEKESPEKLFSIIEKEMQKTKSQKEDMFNEIKQIENEY
                     QQILKNKDVSREMVEEVKKKKEELLSRNETLDNNYENLERQEKEIENEIDLLERRERE
                     VTDNQEKKLIEKERYQKTEKRREIEQKRWQVEDQRNKTKEELQKSEEQYQKYTKLLKR
                     KEELESQKKLLKEKYQQLESKAKDLENLFLRIQDLIEKQKTLEEEKNNLKSEEEKLEK
                     SIVDVLGVEEEEEEEEIINHKKSKEELKKQLEKEKEELKKLREEILKQKESLKRKQEL
                     AFKKKEEEKLKRKTLEEERKAALEFLKREETEKKQEKIKSDVEEIKEKREEVKKRLEE
                     ELKEEEGGKETHQSSLPKPKPAPILPTIKTAKPLSGILILIFLLLAISGILLFFFKWE
                     PSSLPPAPVLPNEQLAELPLPPAAPNPTHKECRGQKCEIVEGKGVDLCQDDTVCQPPL
                     PPMVIGVSEEIELSSLNSDVFISLLDEKFKKEYPPKTFIEIVPKYQGQLLTISQLFEG
                     LDLHPPQDIKDNLETYTLYLYSQEELFSQERRNRLGIAFKIKEGKSGVVRVAMSEWEK
                     VLPDDFLKVHTLWQRGEKASSDFLDNTYNGIRIRYTNFPVPDLSIDWAIKNDILLLTT
                     SRESMWQTINVLK"
     gene            complement(7753..9840)
                     /locus_tag="P5570_01165"
     CDS             complement(7753..9840)
                     /locus_tag="P5570_01165"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRR39352.1"
                     /translation="MLYQTKFAGRELKIETNSFASQASGCALVSFGKTVVLGTATMGG
                     KDIEADFLPLTVDYEERFYAAGRIIGSRYVRREGRPSEEAILLARMIDRTIRPYFPSN
                     LRREVQVVLTVFAFDEENDPEFPSLIAASVALSISNIPWNGPVAGARVAYLTETKELV
                     LNPTYEQRDKAKLDLFIAGIGDETDDVLFNMLDGQAKEALEEETLQAIQFSKDHLKNL
                     FSFQKEIQNKEGKPKIIVPINDKKNLEDLSKKYYNKIKEILFSRKEDNKSKAAMIEEF
                     KTETGIDSSTFEMLEEKIFHQVVLKEGIRPDGRGFDEIRKISCQAGIIPQTHGSGLFC
                     RGLTHVLSIVTLGGPGEELLVEGMEIVGKKRFLHHYNFPPYSSGEVRRMSSPGRREIG
                     HGALAEKALRPMVPDVEKFPYTIRIVSEVLSSNGSTSMASVCASSLALFDAGVPMKKA
                     VAGISCGLIMDGEDETKLISQRDYKLLTDIQGPEDHFGDMDLKVAGTSDGITAIQLDI
                     KVKGLTLEILKQGLEKAKEARNKILAKMNEAISLPRKELSPLAPKILTIKIDPEKIGD
                     VIGSGGKIINEIISETDTSIEIKEDGLVFVTAKDKEKAQKAIDRIYNITREIKVGEVF
                     QGKVKKVMDFGIFIELLPKREGLLHISEISSFRRQTKKDLMQKFKVGDIVSVKVRNID
                     EGGKVSLVLFPKK"
     gene            complement(10026..10526)
                     /locus_tag="P5570_01170"
     CDS             complement(10026..10526)
                     /locus_tag="P5570_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRR39353.1"
                     /translation="MSIIKHPEQRVGVFIDIENLYYSGKNLFKARVDFKQILKDAVGG
                     RKLIRAIAYVIRTESGEEKVFFDALTRLGIETKVKDIQIYNNFKKADWDVGITVDAIQ
                     ISSSLDVVLLLSGDGDFVPLVEHLKSRGKQVEVVAFLKSTSSRLKEAADDFFDIGQSK
                     KYLLKK"
     gene            complement(10649..10897)
                     /gene="rpsO"
                     /locus_tag="P5570_01175"
     CDS             complement(10649..10897)
                     /gene="rpsO"
                     /locus_tag="P5570_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRR39354.1"
                     /translation="MKKEEIIKKYKLHETDTGSAEIQIALLTEEIKNLTEHLKSQKKD
                     NDCRRSLLKKVAKRKKLLDWLKQEDEKRYNNIVKKLKL"
BASE COUNT         3116 a         2069 c         1311 g         4412 t
ORIGIN      
        1 cgcaacaatg ccacgggatc gccacgctcg gtctttgacc tcgctcgcga tgacagtggt
       61 gagggttcgc aatgacaaga aggtaatgtt gcattaaatt cttgaaccca agctagaata
      121 attattaatt tccaatcata aaggccgttt aatttataac ttatctgatt acttatgtca
      181 ccttttgaac gttttaaaaa atcaaatact gagggcaatc tatggattta tattttagcc
      241 ctaggcaaaa acgaagtggt gcctgatgaa aatgtaaaaa aattaatttt tgaaaagttt
      301 ggatttttac cagatagttt tttagtaaat agagttttat atcgtttaag acaagggaaa
      361 tatattgaaa cagaaagatt caaaggaaaa aaagcatata aaacaactga agatggttta
      421 agagaattag aaaaaatgaa ggtcttttgt caggatctac ttcaaaagat tttaaaacaa
      481 ggctaattaa ttaatagaag agctgttttt ttgcaattga atagcagaat gtattcgctg
      541 aagagcagta aaattagcaa gaatagcaac aactattaat acataaacca taaaagtaaa
      601 attaaaaatc cctataatta aagaaataat taataaaatt attctttctc ctcgggataa
      661 aattcctccc tttagttctt gatttaccaa atctttttct ttggcagttg cttttgcata
      721 tgttgtcatc atcgaaccaa acaaaataag aaaaatccaa atgtaagacg gaaataaaat
      781 ttcaggcaat ggtaaaaata acatgccaaa aagcaatatt ccctctacat aacgatcaca
      841 aacagtatct aaataagcac caacctttgt tgaaatattt ttatatcggg ccactgctcc
      901 gtcaacaaaa tccaaaaaac cagcaaaaat aaaaaaaaca attgttaata ttaagttttg
      961 gtttattaaa aaatacaaag aaattaacgc aaaaaaaaca gaaagcagag tccattgatt
     1021 tggagaaagc ggtaaataag aaaaaatcct gcccactttt ttttctaatc ccttaaataa
     1081 atctcttttt aaatctagca taattttaat ggcttttttt attgcttttt ttattttaag
     1141 atataaaatc agcgcgtatt ataaatattg tgaaataata caaatgactc ctattattgc
     1201 acaataaatt ccaaaataat aaagcttccc atttattact acttttttaa ggccctttaa
     1261 agcaaaaaaa ccaacaactg cagcaaagaa aaaaccaaca aagcctccta ttgcctcatc
     1321 agcattaaaa tttaaaattt caggaatttg aagtattgtc gcgcctaaaa tagcaatcat
     1381 ccccagataa aaagaaaaat taaaagcatc ttctttttta atgctgcaaa aaagtgatcc
     1441 cgaaatagta gaaccagagc gagaaacacc aggaagaatt gatagggcct gaaaaagccc
     1501 aactattatc gcatctttag caccaatttc ttttatattt ttttgagaat ttttaacaaa
     1561 acgagtaaag aataatattg ttgctgttat caaaaatgag atacccacca atagaagtga
     1621 attaaatatt gcttctattt tttcttgtaa aagaaaacca acaattacca caggaatagt
     1681 gcctatgatt aaaagtaaaa tcaatttttt atctttaagg acttttttaa tatcgctcca
     1741 aaagaaaaaa ataagggaga ccaatgttcc aaaatgaacc aaagtgtcaa tagcaatagg
     1801 tgcttgtgtg aagccgaaaa gtttttgaaa aattactaaa tgaccagatg aagagatggg
     1861 caagaattca gtaagtcctt gaacaatacc caatattaca gcttgaaaaa tatccatttt
     1921 atttagtatt gaaaattcat ttttaattct tttccatcta taattctttt aagattttga
     1981 cgatgagccc accaaataag ccctactaaa actagtccca acacaaaata aggcaaagaa
     2041 tcaaaaatcg ttataaaaat taatggaaca aatgcaatca ttaaaagatt tgttacacta
     2101 gtaattttaa aaatgagcag caataaaatc caaatgaaaa tccaaaccaa aaataccttc
     2161 caaccaaata acacaattaa aataccaaaa gtagtagcta ctccctttcc acctttaaaa
     2221 gaaagccata ctggaaaaag atggcccaaa acagcaaaaa tagcaactgc tgccacttgc
     2281 cattgagaat ctaaaaactt cactgccaaa aaagtaggaa taacaccttt taaaatatca
     2341 caaatactta cgaccaatcc ccacctccag ccaaaagcac gagaaacatt agctcctcca
     2401 atgttcccgc ttcctacttt tctaatatct acaccttttg cctttgaaat taaataaccg
     2461 ctaggaatag atcccaaaag ataagcgaat attagaagta gaataaaagt tattatcata
     2521 attttaaatt atgaatgata tccgaaaaaa tgaactcgcc tgtaaattta agatgttcga
     2581 gcgccacatt ttgaacaaaa ctttgtttcg ggaatgacca ttaatctttc tttggatatt
     2641 tcttttccgc atctttcaca tatgccgtaa ttcttattct tttttatttt ttctatggct
     2701 ctttctatat ccaataattg aagctctaat cgatactcaa gcggtaaaag gttcgtatat
     2761 tcttccactt catctgcatt ttcgtcttgc tcggaagtgt gagatccaaa ctgaggaaat
     2821 tttgtttccc aatcactggt tttttttcta tcttttttag aaaaagaaga taaaattctt
     2881 tccaattcta ttctttcttc ctccaatttc tcttttaatt cttttaaaag tgatttatcc
     2941 atatctattt taaactctaa gttttaaact ctaaactcta aaaaagaaag ttataaaccc
     3001 aaaaaacgat ttttaaggtt agaagaaaac tttgttttct tctgtcgctt tatcttactt
     3061 gagcaagatt cagcttttga gttttgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnngagt tttgggttta gagtttatta ttttaataca ttaattgttt gccacatact
     3241 ctctcgagaa gtggtaagca atagtatatc attttttatt gcccagtcaa tagataaatc
     3301 tggtacggga aaattggtgt agcggatacg gataccatta taagtattat ctaaaaaatc
     3361 agaacttgct ttctctcctc tctgccataa ggtgtgaacc tttaaaaaat catcgggtaa
     3421 aaccttttcc cattcactca ttgctaccct cacaacacct gattttcctt ctttgatttt
     3481 aaaagcaata cccaaacgat ttcttctttc ttgagagaaa agttcttctt gagagtaaag
     3541 ataaagagta taagtttcga gattatcttt tatgtcttga ggaggatgga ggtctagtcc
     3601 ttcaaaaagt tgtgaaatag tcaataattg accttgatat ttgggtacaa tttcaataaa
     3661 agtttttgga ggatattcct ttttgaattt ttcatccaac aaagatataa acacatcaga
     3721 gtttaaactg cttaattcta tctcttctga cacgccaatc accataggcg gcaatggtgg
     3781 ctggcaaaca gtatcatcct ggcataaatc aactcctttt ccttctacta tctcacattt
     3841 ttgaccccga cactctttgt gagtaggatt gggtgcagct ggtggcaaag gcaattcagc
     3901 taattgctca tttggtaaaa caggtgcagg aggaagtgaa gacggctccc atttaaaaaa
     3961 aaataataaa attccactta ttgccaataa aagaaaaatt aagattaaaa taccactcag
     4021 tggttttgct gttttgatgg ttggcaaaat aggagcaggt tttggtttgg gcaaagaact
     4081 ttgatgcgtt tcctttcctc cttcttcttc tttcaattct tcttctagtc gtttttttac
     4141 ttcttctctc ttttctttta tttcttcaac atcgcttttt attttttctt gttttttttc
     4201 cgtttcctct ctttttaaaa attccaaagc cgcctttctt tcttcttcca aggttttcct
     4261 ttttaatttc tcttcttcct tttttttaaa agcaagttcc tgttttcttt tcaaactttc
     4321 tttctgcttt aaaatctctt ctcgaagttt ttttaattct tctttttctt tttctaattg
     4381 tttttttaat tcttctttac tttttttatg attaattatt tcttcctctt cctcttcctc
     4441 ttctacgccc aaaacatcga ctatggattt ttctaatttt tcttcttctg atttcaaatt
     4501 atttttttct tcttccagtg ttttttgttt ttctatcaaa tcttgaattc ttaaaaaaag
     4561 attttccaag tccttcgctt tgctctctaa ctgctgatac ttttctttta ataacttttt
     4621 ttgactttcc aactcttctt tcctttttaa cagtttagtg tatttttgat attgttcttc
     4681 tgatttttga agttcttctt ttgttttgtt tctttgatcc tcaacctgcc atcttttttg
     4741 ttctatttct cgcctttttt ctgttttttg atatctttct ttttcgatca attttttttc
     4801 ctgattatct gttacctctc tttctcttct ttcaagaaga tcaatctcgt tttctatttc
     4861 tttttcttgt ctttcaagat tttcataatt attatctaat gtttcattac gagataaaag
     4921 ttcctctttt ttctttttaa cttcttctac catctccctt gaaacatctt tgttttttaa
     4981 tatttgctga tattcatttt caatttgctt tatttcatta aacatatcct ctttttggga
     5041 ctttgttttc tgcatctctt tttctattat tgaaaaaagc ttttctggac tttctttctc
     5101 tttttctttt ttaaatcttt ctatttctaa ttttgtttcc tgtgttttgt ttcgaagatt
     5161 atccaattgt gatattaaat ttttcaacaa caactctttt tgcttctttt cttctgaaag
     5221 atttttaagc tcttttaaaa tgttttcttt ttcttcttct agcaattttc tttcttgaat
     5281 tgcctcttta ttttcttttt tgatttcttc tttttgtttt tcttgaataa ttttttcttc
     5341 aaactctttc agcttttttt gattttggaa aatttcactc tttaaagatc ttacttcttc
     5401 aagaagaatc tgagcatttc ttataacatc atctattttt aattcttcct cttcctcttc
     5461 ctcttcctct tcaattcgtt tttccaaacg tgctatctct tcttctatac tttttaaaga
     5521 atcaatactt tcttcatctt cttcggtttg aaatcttttt atttcttcca gtgccccaag
     5581 caattcttct ttctcttttt ttatcttttc taattcaatt attaactgat catcttttga
     5641 atctgatttt tcttcctggg agaaaagaga ggtctctgat ataaattctt tttcaacttt
     5701 ctcttcttcc actggtgcta atttctttcc agcaaaaaca tccttaacat cattttttat
     5761 ttcttgccat tctttttcaa tctcttcttc tttttccttt atttgatctc ttattttttg
     5821 atttcttgtt ttttgatcat taaaagacat aagggcattg tatttttctt ctattaattt
     5881 tagctcttct tttgtttttt tctgttcatc ttcaatctgc cacctttctt tttttatttc
     5941 ttttctttgc tcttccactt tccatctttt ttcttcaagt tccctatttc ctcccaaacg
     6001 ttttatctgg tcttctattt cctgctcttg tgattcaatc tcctcttctc tttgctgtaa
     6061 ttcttttatt ttttcattta attgctctag tttttcctga gttttgtctt ttattgctct
     6121 tgtcttttga atttcatttt ccaatattaa agagagctgc tctattcttt tttgcgcttt
     6181 ttgccttttt tgcgcttttt gttcttcttt ttctttttca atcaaaaatg gcttgatttc
     6241 atcatcaagt gtttgttttt cttcttctag cttttttaat tcttctctca cattttctac
     6301 ttctttatct attttttcta tcgctttttt taattcttct ttttctgttt ttttaaaatt
     6361 aatttccttt tcaacctctt ctcttttttc aatgttttct ttatttaaaa ggtcttcatt
     6421 ttgttgcgta tttgcttgcg ctcgttctgt ctcaaacttc aactcttttt ccttttcttt
     6481 tatttgttgt tctttttctt ctttttgtgc catcaatcgt tccttctctt tccgaagcat
     6541 ttctatcatt ctttttattt ctaacatttg ctgatttgta ttttctttta atctctctaa
     6601 ttcctcctga agagatagga cttcttcttt ttttattgtc ttctcttctt tttttgaaac
     6661 attcccagga ataattcttt ttaaaaatcc ttgagacttc ttaaaattat taatttcttc
     6721 ttgaagctct ttcttttttg catctttttc ctgaatttta tttttcattt ctgcaatctt
     6781 ttgttccttt tctgtttttt gactccttaa atcttccaat tctttattta atttttcttc
     6841 ttctgttact aattgtttta aatcgctttc ccatctgctc tgagattcat taacaatttt
     6901 tatttcttcc tcttcttctt cctcctcaat attttttgaa aaagaaaaat ttatttcatt
     6961 ttcaatgatt tttttaatat cttgaatttt tgcaatgtct ttttccattt cttctttttt
     7021 tgcttttaaa gcattatatt gttcttttgt tttttctcct gaaagttctg gcacatattg
     7081 tctatgtatt tttgcaattt ttgccacttc tttttctttt gactgaaaaa gagattctat
     7141 cttttttatt tcatcgtcta gttgtttatt tatttttatt agctcttttt tgttttcttt
     7201 tttttgagaa attttctctt tttgttcttt aatttttaaa atcaattttt tctcctcttc
     7261 tcttttttct tcttctgatt ttttcttttt taaaagacct tgacgaaatt cttcctttct
     7321 tctctcctct tcctcttttg attcttcctt aagggctgcc attttttctt tttctgcctt
     7381 tattttctct tctttctctc tttctgcctt ttctctttct gcctttctct gcaattcaag
     7441 agcagccatt tttttctctt ctatagcttg tttttttaat tcttcttctc tctttttttt
     7501 caaagccaaa agtgcttttt ctttttcttt ttgctcctgt ttttgtttct gcttttgcaa
     7561 ttgagataat ttttcttcag gaatagaaaa acgggctaaa tctttagcca ttgttcgaat
     7621 ttctaaaaga tgaagttcgt cttcacttct tttcttttgt tcggccctca ctttatcaag
     7681 ataaccttta gaggacttaa tatctttttt gtttttttta aaaagcgcca tatcttaagc
     7741 gagaatcatc gactattttt taggaaaaag aaccaatgaa accttccctc cctcgtcaat
     7801 atttctcacc ttgaccgaaa caatatcacc tactttaaat ttttgcatta aatctttttt
     7861 tgtttgccgg cggaatgaag aaatttcaga aatatgtaaa agtccttctc tttttggtaa
     7921 aagttcaata aaaataccaa aatccattac cttttttact tttccctgga atacttcacc
     7981 cacttttatt tctcttgtaa tattataaat tcggtcaatt gctttttgcg ccttttcttt
     8041 atcttttgcg gtaacaaaaa ctaaaccgtc ttcttttatc tcaatagaag tatctgtttc
     8101 gctaataatt tcattaataa tcttgccacc gcttccaata acatctccaa ttttttctgg
     8161 atcaatcttt atagttaaaa ttttaggagc aagtggcgac aactctttcc gaggcaaaga
     8221 tatggcttca ttcatttttg ccaaaatttt atttcgggct tcttttgctt tttccaaacc
     8281 ctgctttaaa atttctaagg ttagtccctt taccttaata tcaagttgaa tagctgtaat
     8341 gccatcactt gtacctgcta cttttaaatc catatcacca aaatgatctt ctggtccttg
     8401 aatatcagtt aaaagtttat aatccctttg ggatattaat tttgtctcgt cttctccatc
     8461 cattattaac ccgcaagaga taccagcaac tgctttcttc attggtactc ctgcatcaaa
     8521 aagagcaagc gaggaagcac atactgaagc cattgaagtt gaaccattag atgataaaac
     8581 ctcagaaaca attcgaatgg tataaggaaa tttttcaacg tcaggaacca ttggtctcaa
     8641 tgctttctct gctaaagccc catgtccaat ttctcttctg ccaggagagc tcattcttct
     8701 tacctcgcca gaagaataag gaggaaaatt ataatgatgc aaaaaccttt ttttacccac
     8761 gatttccatt ccttcaacca aaagttcctc gcccggtcca cccagggtta caattgataa
     8821 tacatgggtt aatccccgac aaaataatcc cgaaccatgg gtttgaggaa taatgcccgc
     8881 ctgacaacta attttcctta tttcatcaaa ccctctgcca tcaggcctta ttccttcttt
     8941 taaaacaact tggtgaaaaa ttttttcttc aagcatctca aatgtcgaac tatcaatacc
     9001 tgtttctgtt ttaaattcct ctatcattgc ggctttggac ttattatcct cttttcttga
     9061 aaataaaatt tctttaattt tgttgtaata ttttttactt aaatcttcta aatttttctt
     9121 gtcgtttatt ggaacaataa tcttgggctt gccttctttg ttttgaattt ctttttgaaa
     9181 tgaaaagaga ttttttaaat ggtctttaga gaattgtatt gcctgcaatg tttcttcttc
     9241 taaggcctcc tttgcttgtc catcaagcat attaaaaaga acatcatctg tttcatcccc
     9301 tatgccagca ataaataaat ctaattttgc tttatcacgt tgttcataag tagggtttaa
     9361 tactaattct tttgtttctg ttaagtaagc aactcttgct ccagcaactg gcccattcca
     9421 aggaatatta gaaatcgaaa gggccactga agcggcaatt aatgatggaa actcaggatc
     9481 attttcttca tcaaaagcaa aaacagtaag cactacctga acctcgcgcc tcaaattgct
     9541 tggaaaataa ggacgaattg tccgatctat catccttgca agcaagattg cttcctcgct
     9601 tggccttcct tctcttctta catatcgtga tccaataatt cttcctgcag cataaaatcg
     9661 ctcttcgtaa tcaacagtaa gaggtaaaaa atccgcttct atgtcttttc cgcccattgt
     9721 agctgttcct aaaactactg tttttccaaa agagaccagc gcacaaccag atgcctgaga
     9781 agcaaatgag tttgtttcaa tttttaattc ccgacctgca aattttgttt gataaagcat
     9841 aatataaaaa cttctaaatt aacctaatag ccaagttcgt cggcagacga acaaagaata
     9901 aaaagagatt tatctctttt ttcaaagaaa tttttaattt ttaatttaag tttattatta
     9961 aggtttagaa ttttaaagag agacactact cttttaatat acaatctaat ttaattagcc
    10021 atcagtcatt tttttaaaag atactttttt gattgtccga tatcaaaaaa atcatcagct
    10081 gcctctttca atcttgaaga tgtgcttttt aaaaaagcca ctacctctac ctgcttacca
    10141 cgacttttta aatgttcaac caatggtaca aaatcaccat cacctgataa aagcaacacc
    10201 acatcaagcg aagatgatat ttgtattgca tcaactgtaa ttcctacatc ccaatctgcc
    10261 tttttaaaat tattataaat ttgaatgtct tttacttttg tttcaatgcc caatcgcgtt
    10321 agggcatcaa aaaacacttt ttcttcaccg ctttcagtac gaattacata agcaatggct
    10381 ctgatcagct tacgtccccc tactgcatcc ttcaaaattt gtttaaaatc tactcgcgcc
    10441 ttaaaaagat ttttacccga ataataaaga ttttcaatat caataaatac ccccactctt
    10501 tgttctggat gttttataat agacataaat aatattaatt ataaatttca atttataata
    10561 acttctcgcc agcgtcgcta ttaaggatgt atacgtttta atttttccta actcccattg
    10621 cccaataact aattagccct ttttaaaatt acaatttcaa ctttttaact atattattat
    10681 atctcttctc atcttcttgt tttaaccaat caagtaattt ttttcttttg gctacttttt
    10741 ttagaaggga acgacggcaa tcattgtcct ttttctgaga ttttaaatgc tctgttaaat
    10801 ttttaatttc ttctgtcaaa agagcgattt gaatctctgc tgaccctgtg tcagtttcat
    10861 gaagtttata tttcttaata atttcttctt ttttcataat ttaaatggag tagtcactaa
    10921 tatacgaatg tccacaaata tacgaatttg tatattattt taaattcgta tattcgtgat
    10981 tgctccattt tttattttaa cataattt
//
LOCUS       DAOTQD010000015        10472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_5953_length_10472_cov_7.396851, whole genome shotgun sequence.
ACCESSION   DAOTQD010000015 DAOTQD010000000
VERSION     DAOTQD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5953_length_10472_cov_7.396851"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1448
                     /gene="xseA"
                     /locus_tag="P5570_01180"
     CDS             102..1448
                     /gene="xseA"
                     /locus_tag="P5570_01180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRR39355.1"
                     /translation="MKTLQTKTERVFSVSEFLDFLNNILIPQQVVIQGEIGKKIDCRP
                     TYSIFNLLDKKDHTVLKCFCWQDKLENLGIELREGLEIQVLGHPKIYKSQYGSNFEFE
                     AEQVLLVGEGVLKEAFDALKRKLDKEGLFSLDHKRPLPRFCQKIGLITSKYGKGAKPD
                     FEKHLGKFGFEVYFYDVRVEGIFAVNEIVQALYWFNEHMPDLDVVVLIRGGGSWESLQ
                     AFNSEEVARAIFASKIPVMCGIGHESDITIADLVSDVRASTPTDAAKILNENWKMASI
                     NVLEIEKNLSTLFKKALENISQNLDFFEDYFKRTVKKEIFTVKTGLESLIKNLDFNFQ
                     NYFKEFDILSKGFKDNFLKIERLIKNKREETNKIQSDLQKNKDWWYQKIESFLKAERA
                     KIVLSSPYFRLKQGYTITLDEQGKSIKNSEKLKIDQTIKTKFYKGQALSRVKKIES"
     gene            1453..1659
                     /gene="xseB"
                     /locus_tag="P5570_01185"
     CDS             1453..1659
                     /gene="xseB"
                     /locus_tag="P5570_01185"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRR39356.1"
                     /translation="MPEKKLNFSEAYKEIEEINEWFQSEEIDLDEALNKYERGMELIN
                     NCKNRLKEAENRFEEIKKKYGEEK"
     gene            complement(1680..2606)
                     /locus_tag="P5570_01190"
     CDS             complement(1680..2606)
                     /locus_tag="P5570_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRR39357.1"
                     /translation="MAANLPPYFFKLQERLDETADLKEKISILEEMLAVCPKHKGTEK
                     VQKELKTKIAKLKKQKPKKIKSEVFYTVPKKGAGEVVIVGPANAGKSSLLNALTNAQE
                     KVADYPFTTQFPKPAMMPFENILIQLVDTPPLTKDSPGWLKSLIFESDALMAVFDLSN
                     PAVQKQIKEIKKLLEIWRFTNKKIIFVGNKFDLEISKKCFKQLKTTPKEKIVPVSAQK
                     RTGLKELKKKIFETLEIVRVYSKKIGEEVNFENPFIAPKGTKLINLAEKINKDFVLLF
                     KYARLKKKNSPRIQIVGKDYILEDEDIIEIHI"
     gene            2763..3503
                     /locus_tag="P5570_01195"
     CDS             2763..3503
                     /locus_tag="P5570_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HRR39358.1"
                     /translation="MLNTRARRRRAKRGDEGKLQRRKTSSTKVFVPRPETEFWVQKAI
                     KEIKKIKNIPSTDSGDIPFQENLSAESGFKILDIFAGSGCIGIAVLKACPESCQKVDF
                     VDIDEKALQEIKINLKENNIEKERYRIIKSNIFENIKSEKYNIIFANPPYVALERISE
                     VDEEVLENEPWTALFAGSDGMVIIERFLKEVKSHLKKNGIIYLEFDPFQKEKIKNILK
                     KQGFYFCFCKDQFQKTRWVKIYADTKKS"
     gene            3481..4800
                     /locus_tag="P5570_01200"
     CDS             3481..4800
                     /locus_tag="P5570_01200"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRR39359.1"
                     /translation="MQIPKNLNKTLKEKNVKFIRPVFVDILGRMLDFTVPVTRINNII
                     KNGNGFDGSSVEGFARIEESDLIFLPEIKTLKILPWEEKGVDYSWREAIVFGDIIDSQ
                     RKNFEGDTRTLLKNIIKKYEDIGTLKCGAEIEFFIFKDNCLPKHTDEGEYFRSGLYGE
                     IRKETQLMLNKLGIKTEFDHHEGAFSQHEIDLTYGEAIEMADSIILTKFILKRVARKY
                     GLYASFMPKPIEGINGSGMHLHLSLWKNGKNLFFDERKKDLSDMAKSYIAGLIKYGKE
                     IQLVLNQWINSYKRLYPGYEAPTYLVWGTKNRSAYIRVPEYQKGKESATRIEIRSPDP
                     ACNPYIALALIHAAGVQGIKEGLNPPSSIEFDVFHLSEKEKRQLKIEELSPSLERALD
                     FFQKSTLVKETLTPHLYHKFIENKKIECVNFNKSVTDYEIKNYFKAL"
     assembly_gap    4803..4902
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4903..6050
                     /locus_tag="P5570_01205"
     CDS             <4903..6050
                     /locus_tag="P5570_01205"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HRR39360.1"
                     /translation="MINRGIATFTLDYGKCPRWLFERMVKLSRGVIYAIVDEYGPDEF
                     IKRISDPVWFQSLGTVAAFDWNSSGLTTILTAALKEAIRGQEKSLGIFICGGKGKTSR
                     KTPDEIKQWGNVLALPDENVNNLVYNSKMSAKVDNGLIQDGFQIYHHTFFFSQSGAWS
                     VVQQGMNSQNQTARRYHWYSKDVTDLVCEPHTGIATMIKVPTLDLTSRKSEKNREIST
                     KLIQNNYLSLMKDIKILRKHSDSLSKTLALKFKDQELTFLKLENKEFSWHPLIDEDFS
                     RSKYLEKILAKVCERKPKSYEDFLAIEGVGPKTIRALALVAEVIYGAEPSYDDPARYS
                     FAHGGKDATPYPVDRFTYDKTIEFLCHFNSQKEPQKIEDYDYQEIKE"
     gene            6025..6687
                     /locus_tag="P5570_01210"
     CDS             6025..6687
                     /locus_tag="P5570_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HRR39361.1"
                     /translation="MITKKLKNDNKIKTLYKELKDKYDLSEGQWALWCKRKKSKKDKE
                     QIIIEAILTQRINWKNVELSMINLKKARADSLKDIVNIGPQKLSLLIRPSGFYQTKSY
                     YLFHLAQFVLKNYSSVEKMQKENLKKLREGLLTVKGVGKETADDILLYALEKSIFVID
                     EYTRRFVKKYKIAKDLSYDYLQNLFQKNLPQSIRVYQDFHAIIVLEGKPFARGKVENK
                     NI"
     gene            6700..6873
                     /locus_tag="P5570_01215"
     CDS             6700..6873
                     /locus_tag="P5570_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39362.1"
                     /translation="MNAKSLLFIILGIVLLIFGIYGLIFWWWPLFVKVFLACLGPFLI
                     LVGALLIFIGKEE"
     gene            6997..7683
                     /locus_tag="P5570_01220"
     CDS             6997..7683
                     /locus_tag="P5570_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRR39363.1"
                     /translation="MSNDKKIQLSPNSLNLYLECPCCFWLEKRQKIRRPQPYPYALNT
                     AVDVLLKEEFDQYRAKGEPHPLLVQNKIPAKLFSNQGLLNQWRSNFSGIRYYDQKLDA
                     ILYGAVDDIVEFDDGKLAPLDYKSTGSKVPQVYDRFQLQMDIYTFLLEMNGFETPKKG
                     YLAFYVVDKENGFKDRLPFKKELYEIETNPSDIPGIFKEAVLLLKKETPPPHNSDCEY
                     GNWINKITKL"
     gene            7754..8506
                     /locus_tag="P5570_01225"
     CDS             7754..8506
                     /locus_tag="P5570_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRR39364.1"
                     /translation="MKVQKKYILWALALIVLGILLTGFPFLFKKEFDFKEMDFYDGKA
                     VVVSDLHLDSNPRDLTCVGDYLNNENVSLLILNGDVFDKKHKDPLKKEDLDYVKERLG
                     IEDGSSFDIIYLLSSYNHDPYLKLSKDFKEENVDVLESALALKTGNENFYIFHGDYIL
                     SGYNTGIASIVNKLSANLLYEKFAKIVINEGKDNWVILSHSHVPGINQKAKVANTGSW
                     INRFVSSKNTAVLIEVTGGQKSIIDLVKIPCD"
     gene            8529..9815
                     /locus_tag="P5570_01230"
     CDS             8529..9815
                     /locus_tag="P5570_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HRR39365.1"
                     /translation="MKFIGDFHIHSKYSRATSKQMDPEDLDKWAQTKGIKVIGSGDFT
                     HPDWFKNLREKLEPAEEGLYKLSTKYQTSNTKQTTRFILTAEISCIYTKNNKVRKVHH
                     LVLAPDFKTVEKINTQLSWVGNLRSDGRPILGLDSKELLKIVLDSSPDCMLIPAHIWT
                     PWFGVFGSKSGFDSLEECFEDLTSYIYCIETGLSSDPPMNWRLSALDNITLISNSDAH
                     SCLKIGREANVFEDGELNYKSIIEAIKNGGRSTNANCRLVSTIEFFPEEGKYHFDGHR
                     TCGLRLSPQERKKYGGMCPNCGRPLTVGVLSRVDDLADRPEGEKPPNAVPYVSLVPLN
                     EIIGKVFSKDSYTRAVMEEYQKLILAFGSEFEVLMETPIEEISQVSPEVAEGIKKIRE
                     KKIKVLPGFDGEFGKVQILEEEEEDKIKKSNQETLF"
     gene            9844..10152
                     /gene="raiA"
                     /locus_tag="P5570_01235"
     CDS             9844..10152
                     /gene="raiA"
                     /locus_tag="P5570_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRR39366.1"
                     /translation="MKFQIKSTNIVLTESLRNYIEEKIGKCERFIQLENFPLKAYVEI
                     EKITDHHRKGDIFRAEVNMELPGKLLHCDARKNDIYLAINAVRNKLEREIKEYKELKK
                     "
BASE COUNT         3668 a         1319 c         2046 g         3339 t
ORIGIN      
        1 ttggaaattt atgtaataat aatttctgtt ttctgttttc tgacaactga tttctgttta
       61 gtgtttggct atattactct atttgtggta aaatgaagac aatgaaaaca ttgcaaacaa
      121 aaacagaaag ggtttttagc gtatctgaat ttttagattt tttaaataat attctaattc
      181 cgcaacaggt agtaattcag ggggaaatag gaaaaaaaat tgactgccgg cccacttatt
      241 ctatttttaa tttattagat aaaaaagacc acactgtttt aaaatgtttt tgctggcagg
      301 ataaattaga aaatttggga atagaattaa gagaaggatt agaaattcag gttttgggac
      361 atcccaagat atacaaaagt caatatggga gtaattttga gtttgaagca gaacaggtat
      421 tgctagtagg cgagggcgta ttaaaagagg cctttgatgc cctgaaaaga aaattagata
      481 aagaaggatt gtttagtttg gatcataaac gtcccttgcc cagattttgt caaaaaattg
      541 gacttataac ctcaaaatat ggcaagggcg caaaacctga ttttgaaaaa cacttgggaa
      601 aatttgggtt tgaggtctat ttttatgatg ttcgggttga gggaattttt gctgtcaatg
      661 aaatagtaca agcattgtat tggtttaacg agcacatgcc agatcttgac gttgtagttt
      721 tgattcgcgg tggaggaagt tgggagagct tacaagcatt taattcagag gaggtggcac
      781 gggcgatttt tgcttccaag attccagtca tgtgcggcat agggcatgaa agcgatatta
      841 cgattgcaga tttagtgtca gatgtgaggg catctacgcc gacagatgcg gcaaaaattt
      901 tgaatgagaa ttggaagatg gcttcaatca atgttttaga aatagaaaag aatttaagca
      961 cattgtttaa aaaggcattg gagaatatca gtcagaatct tgattttttt gaggactatt
     1021 ttaaaaggac agtaaaaaaa gagattttta cagtaaaaac aggactagag tcattaataa
     1081 aaaatttaga ttttaatttt caaaattatt ttaaagaatt tgatatttta agcaagggat
     1141 ttaaagataa ttttttaaag atagagagat tgataaaaaa taaaagagaa gaaactaata
     1201 agattcaaag cgatttgcaa aagaataagg attggtggta tcaaaaaatt gagagttttt
     1261 taaaagcaga aagggcaaag attgtgcttt cgagccctta ttttcgatta aaacagggtt
     1321 ataccattac cttagacgag caaggaaaga gcataaaaaa ttcagaaaaa ttaaagattg
     1381 atcaaacaat taaaacaaaa ttttataaag gtcaagccct gtcaagggtt aaaaagatag
     1441 aatcataaat ttatgccaga aaaaaaatta aattttagtg aggcgtataa ggagatagag
     1501 gaaattaatg agtggtttca aagcgaggaa attgatttgg atgaagctct taacaaatat
     1561 gagagaggaa tggagttaat aaataattgc aaaaaccgat tgaaggaagc tgagaataga
     1621 tttgaggaaa taaagaaaaa atacggagaa gaaaaataaa ttttttgaaa aagttattat
     1681 taaatgtgaa tttcaatgat gtcttcgtcc tctaagatgt aatcttttcc tacaatttga
     1741 attctaggag aattcttttt tttaaggcga gcatatttaa aaagcaggac aaaatctttg
     1801 tttatttttt ctgctagatt aattaatttt gttccttttg gagcaataaa aggattttca
     1861 aaatttactt cctcgccgat tttttttgag taaaccctta caatttcgag tgtttcaaaa
     1921 attttctttt ttaattcttt aagccctgtt cttttttggg ctgaaacagg tacaattttt
     1981 tcttttgggg ttgtttttaa ttgtttgaaa cattttttgg atatttcaag atcaaatttg
     2041 ttgcccacaa aaataatttt tttattggta aatcgccaga tttctaataa tttttttatc
     2101 tctttgattt gtttttgaac agcaggattt gatagatcaa aaactgccat cagggcatcg
     2161 ctttcaaaaa taagtgattt tagccatcca ggagaatcct tggtaagggg tggagtgtct
     2221 acaagttgaa tgaggatatt ttcaaacggc atcattgctg gcttgggaaa ttgggtggtg
     2281 aagggataat cagctacttt ttcctgggca ttagtgaggg cgtttaaaag agaagatttt
     2341 ccagcattgg caggccccac aattactact tcacctgcac cttttttggg cacagtataa
     2401 aaaacttcgc tttttatctt ttttggtttt tgttttttta gtttagcgat ttttgttttt
     2461 agttcttttt gaaccttttc agtgccttta tgttttgggc aaactgccag catttcctct
     2521 aaaatagaga ttttttcttt taaatcagct gtttcgtcta atctttcctg aagtttgaaa
     2581 aaataaggtg gtaaatttgc tgccataatt atatgtctct ttttagacga tcgtctattt
     2641 gtagacatct agttgtagac aaaaggaaga gcttttattt ggattttttt gtaaatttat
     2701 tgtatcataa aataagttat tttataatgg aaaatattaa atttttaaat tgcaagatta
     2761 atttgttaaa tacgcgagcc cgccgtaggc gggcgaagcg tggagatgaa ggcaaactcc
     2821 aaaggaggaa aacttcatca actaaggttt ttgttccaag accagaaact gaattttggg
     2881 tgcaaaaagc aataaaagaa attaaaaaga taaaaaatat tccttcaaca gattcagggg
     2941 atataccatt ccaagaaaat ttatcggcag agtcagggtt taaaattctt gatatttttg
     3001 cgggatcagg atgcattgga atagcagttt taaaggcatg tcctgagagt tgtcaaaaag
     3061 ttgattttgt tgatattgac gaaaaggcgc ttcaggaaat aaaaattaat ttaaaggaaa
     3121 ataatattga aaaagaaaga tacagaatta ttaaatcaaa tatttttgaa aatatcaaat
     3181 cagaaaaata taatattatt tttgctaatc ctccttatgt tgccttggaa agaatttctg
     3241 aagtagatga agaagtttta gaaaatgaac cttggacagc tctctttgcc gggagcgatg
     3301 gaatggttat aatagaaaga tttttaaaag aagtaaaaag tcatcttaaa aagaacggga
     3361 ttatttattt ggaatttgac ccttttcaaa aagaaaaaat aaagaatatt ttaaaaaaac
     3421 aaggatttta tttttgtttt tgcaaggatc aatttcaaaa aactcgttgg gtaaaaatat
     3481 atgcagatac caaaaaatct taataaaaca ttaaaggaaa aaaatgtaaa atttatcagg
     3541 ccggtttttg tagatatcct tggcaggatg cttgatttta ctgttccagt gacacggatt
     3601 aacaacataa taaaaaatgg caatggtttt gacggttcat cggttgaagg atttgccagg
     3661 attgaggaaa gcgatttaat atttcttccg gaaattaaaa cactaaaaat tttaccctgg
     3721 gaagaaaaag gagtagatta ttcttggaga gaagcaatag tttttggtga tattattgat
     3781 tcccaaagga aaaattttga aggagacaca agaactttat taaaaaatat tattaaaaaa
     3841 tatgaggata ttggcacttt aaaatgcggc gcagaaatag aattttttat ttttaaagat
     3901 aattgtttac ccaagcacac agacgaagga gaatattttc gttcaggtct ttacggagaa
     3961 ataagaaaag aaactcaact aatgttaaat aaattgggta taaaaacaga atttgatcat
     4021 catgagggtg ctttttcaca gcatgaaata gatttgacct atggcgaggc catagaaatg
     4081 gcagatagta ttattttaac taaatttatt ttaaaaagag ttgcaagaaa atatggactt
     4141 tatgcttctt ttatgccaaa gccaattgaa ggaataaacg gaagcggaat gcacctacat
     4201 ttatctttgt ggaaaaatgg gaaaaattta ttttttgatg aaagaaaaaa agatttgagc
     4261 gatatggcaa aatcttatat tgccggttta ataaaatatg gaaaagaaat acagcttgtt
     4321 ttaaatcaat ggataaattc ttacaagcgg ctttacccag ggtatgaagc gcctacttat
     4381 ttagtatggg gcaccaagaa tcgatctgcc tatataaggg tgccagagta tcaaaagggt
     4441 aaagaaagtg ctacgagaat tgagataaga agtccggatc cagcttgcaa cccttatata
     4501 gcacttgctc ttattcatgc tgctggtgtt caggggatta aagaaggatt aaatccacca
     4561 tcttcaattg aatttgatgt tttccattta tcagaaaaag aaaaaagaca attaaagata
     4621 gaagaacttt cgccaagctt agaaagggca ttggattttt ttcaaaaaag caccttggta
     4681 aaagaaacat taacccctca tctttaccat aagtttattg aaaacaaaaa gattgaatgc
     4741 gtcaatttta ataaatccgt gacagattac gaaataaaaa attactttaa ggccttatga
     4801 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttatgatt aatcgaggaa
     4921 ttgctacatt tacattggat tatggaaaat gtccacgttg gcttttcgaa cgcatggtaa
     4981 aactttcgcg cggtgtaatt tatgcaattg ttgatgaata cggtcctgat gaatttataa
     5041 aaagaatatc tgatccggtt tggtttcaat ctttgggcac agttgcggct tttgattgga
     5101 attcttctgg cttgaccaca attctaactg cagctttaaa agaagcaatt agaggccagg
     5161 aaaaatcgtt gggtattttt atttgcggag gaaaaggtaa aacatcaaga aaaacacctg
     5221 atgaaattaa gcaatgggga aatgttttag ctttgccaga tgaaaatgtt aataatttag
     5281 tttacaattc aaaaatgtct gccaaggtag ataacggatt gattcaagat ggttttcaga
     5341 tttaccacca cacctttttc ttttcacaaa gcggtgcttg gagtgtagtt caacaaggaa
     5401 tgaattctca aaatcaaaca gcccggcgtt accattggta ttcaaaagat gttactgatt
     5461 tggtttgtga accccataca ggcattgcga ccatgataaa ggtgccaacc cttgatctta
     5521 cttcacgcaa aagcgagaaa aatcgtgaaa taagcactaa attgattcag aataattatt
     5581 tatctttgat gaaggatatt aaaattttaa gaaaacactc tgatagtttg tctaaaactc
     5641 ttgctttaaa gtttaaggat caagaattaa cttttttaaa attagaaaac aaagaatttt
     5701 cgtggcatcc gctaatagat gaagattttt caaggagtaa gtatttggaa aaaattttag
     5761 ccaaggtgtg tgaaagaaaa ccaaaaagtt atgaggattt tttagcgata gaaggcgttg
     5821 gtccaaaaac cattagggct ttagctttgg tagcagaagt gatttatgga gcagagccga
     5881 gttatgatga tccagcccgt tattcatttg ctcatggcgg aaaggatgcg acaccttatc
     5941 ctgtggatcg ttttacatat gataaaacta tagaatttct ttgtcatttt aattcccaaa
     6001 aagaaccaca aaaaatagaa gattatgatt accaagaaat taaagaatga caataaaata
     6061 aaaacactgt acaaagaatt gaaagacaaa tatgatcttt ctgaaggaca atgggcgtta
     6121 tggtgtaaaa gaaaaaagag caaaaaagac aaagaacaaa ttattattga agccattttg
     6181 acccagagga tcaattggaa gaatgtagaa ctttcaatga ttaatcttaa aaaagcacga
     6241 gctgattcat taaaggatat tgtaaatatt ggacctcaaa aactttctct tcttatcagg
     6301 ccatcgggat tttatcaaac aaaatcttat tatcttttcc acttggctca gtttgtttta
     6361 aaaaattata gtagtgtgga aaaaatgcaa aaagaaaatc ttaaaaaatt aagagaagga
     6421 ttattaaccg tgaaaggggt tggcaaagaa acagcagatg atattttgct ttatgctctt
     6481 gaaaaatcta tttttgtgat tgatgaatat acacggcgtt ttgttaaaaa atataaaatt
     6541 gccaaagatc tttcttatga ttatcttcaa aatttatttc aaaaaaatct gcctcaaagc
     6601 attagggtct atcaggattt tcatgctata attgttttag agggaaaacc ctttgcaagg
     6661 ggaaaggtcg aaaataaaaa tatataactt attaaatcta tgaatgctaa aagtttatta
     6721 tttataattt taggaatagt gcttttaatt tttggaattt atgggttgat tttttggtgg
     6781 tggcctcttt ttgttaaggt gtttttggct tgtcttgggc ctttcttaat tcttgtagga
     6841 gcattgttaa tttttattgg caaagaagaa taattttaaa aaacaagttg acaagtattt
     6901 ttatttttcg tcaatttatg cggataacga tgaattcttt cagtagcaat gtaaggtttg
     6961 gttggtatta ttgtgagaat aaatacgaat aaaaatatgt caaatgataa aaaaattcaa
     7021 ctatcgccga attctttaaa tctttatttg gaatgtccat gctgtttttg gttagagaaa
     7081 agacaaaaaa ttagaaggcc tcaaccatat ccttatgcct taaatacggc agtagatgta
     7141 ttattaaaag aagaatttga tcaatatagg gcaaaagggg agccgcatcc tttattggtg
     7201 caaaataaaa ttcctgctaa gcttttttct aatcagggat tactcaatca atggcggagc
     7261 aattttagcg ggattcgtta ttatgatcaa aaattagatg ctattttata tggggcggta
     7321 gatgatatag tggaatttga tgacggcaaa ttagctcctc ttgattacaa atcaaccggc
     7381 agcaaagttc ctcaggtata tgatcgtttt caattgcaaa tggatattta tacttttctt
     7441 ttagaaatga atggatttga gacacctaaa aagggttatt tggcttttta tgttgttgat
     7501 aaggaaaacg ggtttaaaga tcgtttgccc tttaaaaaag aattatatga aatagaaacc
     7561 aatccttcag atattccggg gatttttaaa gaagctgttt tgcttttaaa aaaagagaca
     7621 ccgccacccc ataattctga ttgtgaatat ggtaattgga ttaataaaat tactaaatta
     7681 taaatattct ttttatgcca tgttcacttt ttgtttatac atattttaaa aacgatcgta
     7741 gaaaagttat gatatgaagg ttcaaaagaa atatatttta tgggcgctgg ctttgattgt
     7801 tttaggaatt ttattaactg gtttcccttt tctttttaaa aaagaatttg attttaaaga
     7861 gatggatttt tacgatggaa aagcagtggt cgtttcagat ttacatttgg attcaaatcc
     7921 tcgtgattta acttgtgtcg gtgattattt aaataatgaa aatgtgtctt tacttatttt
     7981 aaacggcgac gttttcgata aaaagcataa agatccatta aaaaaagaag acttagatta
     8041 tgtaaaagaa agattaggga ttgaggatgg ttcctctttt gatataattt atttactgtc
     8101 ttcttataat cacgatcctt atttgaagtt gagtaaagat ttcaaagaag agaatgttga
     8161 tgttttggaa agcgcccttg cacttaaaac cggtaatgaa aatttttata tttttcacgg
     8221 tgattatatt ttaagcggtt ataatacggg catagcaagt attgttaata aattaagcgc
     8281 aaatttactt tatgagaaat ttgccaagat agtaataaac gaaggaaaag ataattgggt
     8341 tattttgagt cattcccatg tgccagggat taatcaaaaa gcaaaagtag ctaataccgg
     8401 ctcttggata aatagatttg tatcaagcaa gaatacagca gttttaatag aagtcacagg
     8461 agggcaaaaa tcaataattg accttgttaa aattccttgt gattaagtga gatttattaa
     8521 aatattttat gaagtttata ggagattttc atattcattc caagtattct agagccacct
     8581 caaagcagat ggatccagaa gatcttgata agtgggcaca aacaaaggga ataaaagtga
     8641 ttggtagtgg tgattttact catcctgatt ggtttaaaaa tttaagagaa aaattagaac
     8701 cggctgagga aggattatat aaattgagta ctaaatatca aacatcaaat accaaacaaa
     8761 ccactcgatt tattttaaca gctgagatta gttgtattta tacaaaaaat aataaggtaa
     8821 gaaaggttca tcatttggta ttggcgccgg attttaaaac tgtggaaaag attaatactc
     8881 agctttcttg ggttggaaat ttaagatctg atggaaggcc aattttagga ctggattcaa
     8941 aagaattatt aaaaattgtt ttggactctt cgccagattg tatgttgatt cctgctcata
     9001 tttggactcc atggtttggg gtttttggat caaaatctgg atttgattca ttagaagaat
     9061 gttttgagga tttaacgtct tatatctatt gtattgaaac cggtctttct tcggatccac
     9121 caatgaattg gcgattgtca gcgttagata atattacctt gatttcaaac agcgatgctc
     9181 actcttgtct taaaattggt agggaagcta atgtttttga ggatggggaa ttgaattata
     9241 aaagcattat tgaagcaatt aaaaatggcg gaagatcaac aaatgcaaat tgtcgattgg
     9301 tttctaccat tgaatttttt ccagaggaag gtaaatatca ttttgatggt catcggactt
     9361 gcggattacg attatcaccg caagaaagaa aaaaatatgg cgggatgtgt ccgaattgcg
     9421 gaagaccatt gacagtggga gttctttcaa gagtggatga tttagctgac agacccgaag
     9481 gagaaaaacc acctaatgcc gttccttatg tgagcttggt gccattaaat gaaattattg
     9541 gtaaggtgtt ctcaaaggat tcatacacaa gggcagtaat ggaggaatat caaaaattga
     9601 ttttggcctt tggtagtgag tttgaggtat taatggaaac gccaatcgag gaaatctctc
     9661 aagtttcacc agaagtggct gaaggaataa aaaaaattag agaaaaaaag ataaaagtat
     9721 taccgggatt tgatggagag tttggtaagg ttcaaatttt agaagaagag gaagaagata
     9781 aaataaaaaa atcaaaccaa gaaacattat tttaatgttt tttggtgttg gtttaataaa
     9841 aatatgaaat ttcaaataaa aagtactaat attgttttaa ctgaaagttt aagaaattac
     9901 attgaagaaa aaattggaaa atgtgaaaga tttattcagt tggaaaattt tccattaaag
     9961 gcttatgttg aaatagaaaa gattacagat catcatcgca aaggagatat ttttagagca
    10021 gaggttaata tggaattacc agggaagctt ttacattgtg atgcaagaaa aaatgatatt
    10081 tatttagcaa ttaatgcggt tcgaaacaaa cttgaaagag aaataaagga atataaagag
    10141 ttaaagaaat aagattaaaa atcaattttt aattttcaaa aactgtcatc gcgaccactc
    10201 gctttttact aaggtgaggg agaaatccca ttgtgcgtgc actgtcatcg cgaacgagag
    10261 agcgaagcga gctcgtcgaa aagaaaatga aattttcttt gagagcccga agggcgtggc
    10321 gatcccgttg cattgttgcg ggcttcgccc gagattgctt cgtcgccttg gctcctcgca
    10381 aagacagtgg tgggtgggta atgacatccg tgtccactgt catcgcgagg aggtcttata
    10441 ggccgacgtg gcgatcccgt agcaaagaca gt
//
LOCUS       DAOTQD010000016        10085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_6236_length_10085_cov_8.638983, whole genome shotgun sequence.
ACCESSION   DAOTQD010000016 DAOTQD010000000
VERSION     DAOTQD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6236_length_10085_cov_8.638983"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..701
                     /gene="nusG"
                     /locus_tag="P5570_01240"
     CDS             156..701
                     /gene="nusG"
                     /locus_tag="P5570_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRR39367.1"
                     /translation="MSRQQVEQEKNWYVLHTYSGYEETVARNLKQRIESMGMEDKIFN
                     VIVPKEKKIKIKNGKREVVEEKIYPGYVLVEMIVTDDSWYVVRNTPRVTGFIGAGTVP
                     IPVSKEEIDRLQKRMGEKEPEYKIDVRPGDLVKITDGPFKDFDGKVFEIDEERGKIKV
                     LVNMFGRDTPVELDSLQIKKV"
     gene            771..1196
                     /gene="rplK"
                     /locus_tag="P5570_01245"
     CDS             771..1196
                     /gene="rplK"
                     /locus_tag="P5570_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRR39368.1"
                     /translation="MPKKILTIIKLQIPAAKANPAPPIGPALGQHGLNIAEFCQKFNA
                     ATKDKVGDIIPVEITVFEDRTYEFKLKTPPASALLKKAAGIEKGSGEPHKTKVGSVTE
                     AQIEEIAKKKMEDLNTTDLEAAKKIIKGTARSMGITVSK"
     gene            1258..>1767
                     /locus_tag="P5570_01250"
     CDS             1258..>1767
                     /locus_tag="P5570_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39369.1"
                     /translation="MQKKRKIIFAIFIVALGVGLRIFINEKINIPNFEAVTALSLLAG
                     SFLGGIFGAIIPLLIVFFSDLYFGNTSVYIFTWVAYALIGIFGALLKRSSKYYFLKIT
                     GMGILSVVFFYLFTNFGWWLTFEMYPLTLQGLIECYIAGLPFLKNQLFSSLIFVPAFA
                     LIFSLVENRE"
     assembly_gap    1768..1867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1868..2443
                     /gene="dcd"
                     /locus_tag="P5570_01255"
     CDS             <1868..2443
                     /gene="dcd"
                     /locus_tag="P5570_01255"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRR39370.1"
                     /translation="MILSDRDIKKYLKKGKIKISPEPNLEEQLGPCSIDLHLGNEFKI
                     FRPSQYPYLDLKRGTNFEDLMEEVKIKDGAPFILQPKDFLLATTKEELALDNDLMARL
                     DGRSSLGRLGIVVHSTAARFDPGWEGKAVMELGNLGIMPVVLYSGMRICALTFETLSS
                     PCETSYSKKKGQKYYKQESPKASKINQEFLK"
     gene            2448..3299
                     /locus_tag="P5570_01260"
     CDS             2448..3299
                     /locus_tag="P5570_01260"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF016124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit A"
                     /protein_id="HRR39371.1"
                     /translation="METNLNDWPPIDGRYEVGNKTSPVAVCTNASIDEIKIDPQKIAI
                     IGKCVTENIGIEKMIQNIVSNPNIRYLVLCGRESNGHFVGQAIESLIENGVDENKRII
                     GAKGNMPYLRNLDKETIETFREQVIPVNLMEETNGKKIEKVIDELLKIRPQKMKAKGI
                     KIEKVEEIKASPSYWIEDPKGYFVISLDNNRKKIVVEYYKKGKLNKKIIGNNAKEICD
                     TIARLDLIGDFKQTKEHSMYLGRELQKAEIALKQGLTFEQDQELKINKVDNLTQQEPI
                     DEYGWYD"
     gene            3353..4129
                     /locus_tag="P5570_01265"
     CDS             3353..4129
                     /locus_tag="P5570_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRR39372.1"
                     /translation="MYDTKIIEAKTLGEAWEKACILMMKEGYNRYVEAPEYKTETKDL
                     PMMIKVSEPFLEPRLSPKVSTTREQADDYSNKLIFGSGKENENSFDYTYYSRLRCYPD
                     CKVRAFLPSVTETKEELEEEVKKIISDGKCCVERIDQVEKAIKILKKDPTRRTVVMHT
                     WIPYRDLMKFGPKRENTSSPCVVLVYPQIVDGKLHLFVVMKTNDLYNAWPENAYALTN
                     LQKYMADEIGVETGTYTHFSVSMQIYQDMYSEVKNKFNLK"
     gene            4129..4734
                     /gene="tmk"
                     /locus_tag="P5570_01270"
     CDS             4129..4734
                     /gene="tmk"
                     /locus_tag="P5570_01270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRR39373.1"
                     /translation="MFKNPYPGKFIVFEGIDGSGKSTQAGLLFHYLKERKKRVHSTSE
                     PTQYLIGGLIKSWLAKDWQSTPDCLQLLFAADRAYHLDKEIIPLLKKGVTVVCDRYSL
                     STLAYGALELDLDWLITLNQNFIAPDVTFLLNTPPKICLERMQKERFFLNLFEEEKKL
                     KKIWQNYQRLTKKYKNIYTINGEKSIEEVSKEIKKILKIKN"
     gene            5046..6521
                     /gene="gatB"
                     /locus_tag="P5570_01275"
     CDS             5046..6521
                     /gene="gatB"
                     /locus_tag="P5570_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRR39374.1"
                     /translation="MLYQPVIGLEIHCELKTKTKMFCSCLNDPEETQPNFNICPICMG
                     HPGTLPTINKRAVEMVIMVGRALNCEIAQETYFERKNYFYPDLPKGYQISQYAVPLCS
                     GGYLKIPNTDRTIRITRIHLEEDTGKLLHEENSESSLIDFNRASIPLMELVTEPDIHS
                     AKEARQFAEELQLILRYLGVSDADMEKGEMRVEVNISLSQKSNLKSQNLGTKVEIKNL
                     NSFRAVEKASEYEIDRQSKILDKGEKVIQETRGWDDIKGKTFSQRVKEQANDYRYFPE
                     PDLPPFQLSNFALQLSKSMPELPQQKRERFQKEYGIGPKETEILVQNKELADYFENVI
                     SELKYWAKIKEVSRDKVSSLVKLAVNYLESDLLGIMKEKNTPIKDLKITPENFAELIT
                     ILWQGEISSRAGKDVLLEMFKTGADPTYIIKDKNLKQVSDEKAIEGIVKKIIKDNPKP
                     VEDYKKGKENALQFLVGQVMKESKGKANPDKAREILKSLLK"
     gene            6669..7022
                     /locus_tag="P5570_01280"
     CDS             6669..7022
                     /locus_tag="P5570_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39375.1"
                     /translation="MEETIRSIGMERFFLLVLRDLLNKEEDQMLLVDWLNAAFMNTSL
                     QKEDLEFLEIIINSLMEGYFTAGKFFSEKAIDLEEKKKLPNKVVLIDGQRIETRWIDK
                     KEEELMKVLEIISGQ"
     gene            complement(7425..>8040)
                     /gene="recR"
                     /locus_tag="P5570_01285"
     CDS             complement(7425..>8040)
                     /gene="recR"
                     /locus_tag="P5570_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRR39376.1"
                     /translation="MVPKQIKKVVDIFSQFPTIGERTATRFALYLLSLPKEKVEKICQ
                     EILNLKKEIKLCSFCFNPFSPRNKETLCPICLDKKREEIICVVGKETDLWQMEKMKKY
                     NGRYFILGGELEFLKENPLGKMRIEELKKRIEEFKPKEIVLALNPTAGGNLTMDYISK
                     ILKPLITEREQSIKITRLGRGLPTGGEIEYADEETIEKALENRG"
     assembly_gap    8041..8140
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8207..10000)
                     /gene="dnaB"
                     /locus_tag="P5570_01290"
     CDS             complement(8207..10000)
                     /gene="dnaB"
                     /locus_tag="P5570_01290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRR39377.1"
                     /translation="MADVIKIPPQGIEAEKALLGSLMMDKEGMIKVIDLVRPEDFYKN
                     NHGKIYQAMLDIFEKNEPIDLLTLTQRLKEKEELEEIGGAAYLSEILSSVPTPSHIMY
                     YAKIVREKKVRRGLIQVSEEISTLGYQEEKDIDLILDEAEKKIFGITQESLTQSFLPI
                     KTELDGAFERIDKIHSQKGALRGVTTGFPKLDDMLAGLQRGDFILLASRPSLGKTSLA
                     LDIARAAALKENQTVGMFSLEMSKEQLVDRLIASQAGISLWKLRTGKLSADEDFVKIQ
                     DALSALSQAPIYIDDAASSTVLQMRAMSRRLQAEKGLGLIIIDYLQLIESRSQSENPV
                     QQVTEISRSLKSLAKELNVPVLALSQLSRAVEHRPDQRPKLSDLRESGCLTGDTLIQK
                     ADSGERVSMKELAQRKFQEPIRVLALDDNNKLRPSKMTKVFYSGKKEVFELATESGRK
                     IKASANHPFFKLEGWKRLDELKIGDSIAIFNIPNIQMLATSDIYWDKIVSIKLLGVED
                     VYDATVENYHNFLANDIVVHNSLEQDADVVLFIYRPNIKESKTELIIAKHRNGPIGKV
                     DLYFNQDLASFRELEKSYEDEENERSEEEIM"
BASE COUNT         3343 a         1564 c         1905 g         3073 t
ORIGIN      
        1 acgtccttcg ggctctcaaa gaaaatttca ttttcttttc gacgagctcg cttcgctcgc
       61 tcgttcgcga tgacagtggt gaggggtcgc gatgataaga aggtaatgtt gcgttaaatt
      121 cttgaaccta atgagagttt aaaaattaga ttattatgtc tcgtcaacaa gttgaacaag
      181 aaaaaaattg gtacgtgctc cacacttatt ctggttatga agaaacagtt gctcgcaatt
      241 taaagcaacg tattgaatct atgggaatgg aagataagat ttttaatgtg attgttccta
      301 aagaaaaaaa aataaaaata aagaatggaa aaagagaggt agtggaagaa aaaatttatc
      361 ctggatatgt tttggttgaa atgattgtaa cagatgattc ttggtatgta gtgagaaaca
      421 caccgcgagt tactggtttt attggcgctg gcacagtacc tatccctgtt tcaaaagaag
      481 aaatagatcg gctccaaaag agaatgggtg aaaaagaacc agaatataag attgatgtaa
      541 ggcccggtga cttggtaaag attactgacg ggccatttaa ggattttgac ggaaaggttt
      601 ttgaaattga cgaagagaga ggaaaaataa aagttttggt aaatatgttt ggtcgagata
      661 cgccagtaga attggattct ttacaaatta aaaaagttta aaacataatt ctaatttacg
      721 atgaaaggag caaagaaata aatttcttct ttcgcttctc tatactattt atgccaaaaa
      781 aaattcttac aataattaaa cttcagattc ctgctgctaa ggcaaatcct gctcctccta
      841 ttggacctgc cttgggtcag cacggtttga acattgctga attttgtcaa aaatttaacg
      901 cagctacaaa agataaggta ggtgatatta ttccagttga aattactgtt tttgaagata
      961 ggacatatga gtttaaatta aaaacaccgc ctgcttctgc attgcttaaa aaagctgctg
     1021 gtattgaaaa aggttctggc gaaccccata aaacaaaagt aggtagtgta accgaagctc
     1081 agattgaaga aattgctaaa aagaaaatgg aggacttaaa taccactgat ttggaagcag
     1141 caaaaaaaat tataaaaggc acagcgcgat caatgggtat tacagtatct aaataatcag
     1201 gcctgctttg ctttggtacg tccgaattat tccaatgata ttgaaattgt tatttttatg
     1261 cagaaaaaaa gaaaaattat ttttgcaatt tttattgtgg cattgggcgt aggtttgcgg
     1321 atttttataa atgagaagat aaacattccc aactttgaag cagtaacagc tctttcattg
     1381 ttggctggtt catttttggg tggaattttt ggggcaatta ttcccttatt gattgttttt
     1441 ttctctgatc tttattttgg caatacttcg gtttatattt ttacctgggt ggcttatgct
     1501 ttgattggta tttttggcgc tttgcttaaa agaagctcaa agtattattt tttaaagatt
     1561 actggtatgg gaattttatc agtagtcttt ttttatcttt tcaccaattt tggttggtgg
     1621 cttacttttg aaatgtatcc attgaccttg caaggattaa ttgaatgtta tattgctgga
     1681 ttaccatttt taaaaaatca attattttca tctttgatat ttgttcccgc ttttgctttg
     1741 attttttcat tggttgaaaa cagagagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnatg attctttctg atcgcgatat taaaaaatat ttgaaaaagg gaaaaataaa
     1921 aattagcccc gaaccaaatt tagaagagca actaggacct tgttcgattg atttgcatct
     1981 gggaaatgaa tttaaaattt tcaggccctc gcagtatcct tatttagatt tgaaaagagg
     2041 aaccaatttt gaagatttaa tggaagaggt aaaaataaaa gacggagccc cctttatctt
     2101 gcagccgaaa gattttcttt tagctactac aaaagaagaa ttggctttag acaatgattt
     2161 aatggcacga cttgatggta gaagttctct gggaagattg ggaatagtag tacattcaac
     2221 agctgctcgt tttgatccag gatgggaagg gaaagcagta atggagttag gaaacctggg
     2281 gattatgcca gtggttttat attctggaat gcgtatttgc gctttaactt ttgaaaccct
     2341 ttccagtccc tgcgaaactt cctattctaa aaagaaagga cagaaatatt ataaacaaga
     2401 atcgcccaag gccagtaaaa ttaatcaaga gtttttaaaa tagatctatg gagacaaatc
     2461 ttaatgattg gccacctatc gatggaagat atgaggttgg caacaaaaca tcgccggttg
     2521 ctgtttgtac taatgccagt attgatgaaa taaaaattga tcctcaaaaa attgccatta
     2581 ttggaaagtg tgttactgaa aatattggaa ttgaaaaaat gattcaaaac attgtttcta
     2641 atcccaatat tcgctatttg gtgctttgcg gtcgcgagtc caatggtcat tttgtaggtc
     2701 aggcaattga aagtttaatt gaaaatggcg ttgatgagaa taaaagaatt attggtgcaa
     2761 agggcaatat gccttattta agaaatcttg ataaagagac aatagaaacc tttagagaac
     2821 aagtgatacc agtaaattta atggaggaaa ccaatgggaa aaaaattgaa aaggtgatag
     2881 atgaattatt aaaaatcagg cctcaaaaga tgaaagcaaa aggaataaaa attgaaaaag
     2941 tagaagagat aaaagcaagc cccagttact ggatagaaga ccccaagggt tattttgtaa
     3001 tttctcttga caacaatagg aaaaaaatcg ttgttgaata ttacaaaaaa ggaaaattga
     3061 acaaaaaaat tattggcaac aatgctaaag aaatttgtga tactatcgct cgtttggacc
     3121 tgatcggtga ttttaaacaa acaaaagaac attcaatgta tttaggtcgg gaacttcaaa
     3181 aagcagagat cgctttaaaa cagggattaa cctttgagca agatcaagaa ttaaaaataa
     3241 acaaggtcga taatttaaca caacaagaac caatcgatga atatggttgg tatgattaga
     3301 atgtagaatg tagtttacaa ctctctacat tttactttcg actatccaat ttatgtacga
     3361 tacaaaaatt attgaagcaa aaacattagg cgaggcgtgg gaaaaggcct gtattttaat
     3421 gatgaaagag ggctataatc gttatgttga agcgccggaa tacaaaaccg aaaccaaaga
     3481 tttaccaatg atgattaagg tttcagagcc atttttagag ccacggcttt ctccaaaagt
     3541 aagcactacc cgcgagcagg cagatgatta ctcaaacaaa ctcatttttg gcagtggcaa
     3601 agaaaacgaa aatagttttg attatactta ttattcacgt ttacgctgtt atccagattg
     3661 caaggtaagg gcttttttac ctagcgtaac agaaacaaaa gaagagctgg aagaagaagt
     3721 gaaaaaaatt atttctgatg gaaagtgttg cgttgagcgt attgatcagg ttgaaaaagc
     3781 cattaagatt ttgaaaaaag atcctactcg gagaacagta gtaatgcaca catggattcc
     3841 ttaccgcgat ttaatgaaat ttggacccaa aagagaaaac acttcttctc cctgtgtggt
     3901 attagtttat cctcagattg ttgatggaaa attgcattta tttgtggtga tgaaaactaa
     3961 tgatctttac aatgcttggc cagagaatgc ttacgcctta acaaatcttc aaaaatatat
     4021 ggctgatgag attggtgttg aaacaggcac ctatacccat ttttcagtca gtatgcagat
     4081 ttatcaagat atgtacagtg aggtaaaaaa taaatttaat ttaaaataat gtttaaaaat
     4141 ccttatccag gaaaatttat tgtttttgag ggaattgatg gatcaggaaa gtccactcaa
     4201 gctggtttac tgttccatta tttaaaagaa agaaaaaaaa gggtccactc tacttcagag
     4261 cccactcaat atttaatagg gggactcatt aagagttggc tggcaaagga ttggcaaagt
     4321 acaccggact gcttacaact tttgtttgct gctgacaggg catatcattt agacaaagaa
     4381 ataattcctt tattaaaaaa aggtgttact gttgtttgcg atcggtattc tctttcaaca
     4441 ttggcatatg gtgccttgga gctggattta gattggctga ttactcttaa ccaaaatttt
     4501 attgcgcctg atgttacctt tcttttaaat actcctccaa aaatctgcct agaaagaatg
     4561 caaaaagagc ggttctttct taaccttttt gaagaagaaa aaaaactaaa aaagatttgg
     4621 cagaactacc agagattgac taaaaaatat aaaaacattt atactattaa tggcgagaaa
     4681 tccattgagg aagtatcaaa agaaatcaaa aaaattttaa aaattaaaaa ttaaggtaga
     4741 tttaagtttc taacgcaaca atctttatta gaatgtagaa tgtagaatgt agtgtctttg
     4801 cgagggaggc gttagccgac cgaagcaatc tcagagtatg tttgtggacg tgccacggga
     4861 ttgccacgcc ctactgcggt agggctcgca atgacaaagt ataaaatact gtctttgcga
     4921 ggagcgtagc gacgaagcaa tctcgagcga acagggtgag ctcgttgaaa agaagataaa
     4981 atcttctttg aacgttcgtt cgcgatgaca gaaattttaa aaattaaaaa ttaaaaatta
     5041 atagtatgct ttatcagccg gttattggtc ttgaaatcca ttgtgaattg aaaaccaaaa
     5101 caaaaatgtt ttgctcttgt ttgaatgatc cggaagaaac ccaacccaat tttaatattt
     5161 gtccaatctg tatgggtcat ccaggaaccc ttccaacgat taacaagaga gcagtagaaa
     5221 tggtaataat ggtgggcagg gctttaaatt gtgaaattgc ccaggaaacc tattttgagc
     5281 gcaaaaatta tttttatcca gatttgccca aaggatatca aatatcgcag tacgccgttc
     5341 cattatgttc aggtggatat ttgaagattc ccaacactga tcgaacaatt aggataacaa
     5401 gaattcacct tgaagaagac acaggaaagc ttttgcacga ggaaaactca gaaagcagtt
     5461 tgattgattt taatcgggcg agtataccat taatggaatt ggtaactgaa ccagatattc
     5521 attctgccaa agaggcccgt caatttgccg aagaacttca gttgatttta agatacctgg
     5581 gagtttctga cgctgatatg gaaaaaggtg aaatgcgggt agaagttaac attagtttat
     5641 ctcaaaaatc aaatctcaaa agtcaaaatc taggtacgaa agttgagata aaaaacctga
     5701 actcttttcg cgcagtagaa aaagcaagtg aatatgaaat agacagacag tcaaaaattt
     5761 tggataaagg tgaaaaagtt attcaagaaa cacgtggatg ggatgatata aaaggcaaaa
     5821 ccttttctca acgcgtaaaa gaacaggcaa atgactatcg atattttccc gaaccagacc
     5881 ttccaccatt tcaactttca aatttcgccc ttcaactttc aaaatctatg ccagaactac
     5941 cccaacaaaa aagagagcgt tttcaaaaag aatatggaat tggtcccaag gaaacagaaa
     6001 tactggttca aaataaagaa ctggctgatt attttgaaaa tgtaatatca gaattaaaat
     6061 attgggcaaa gataaaagag gtctcccggg acaaggtttc ttctcttgta aaattagcag
     6121 taaattattt ggaaagcgat cttttgggaa taatgaagga gaaaaatacc ccaatcaagg
     6181 atttaaaaat tactcctgaa aattttgctg agttgataac tattttatgg caaggcgaga
     6241 tttcttcacg cgctggtaag gatgtattat tagaaatgtt caaaaccggc gctgatccaa
     6301 cttacattat aaaagataaa aatttaaaac aagtgagtga tgaaaaagcg atagaaggaa
     6361 ttgttaaaaa aattatcaag gataatccaa aaccagttga ggattataaa aaaggcaaag
     6421 aaaatgccct gcagtttttg gtaggccagg taatgaaaga aagtaaaggc aaggcaaacc
     6481 ctgataaggc aagagaaatt ttgaaatctt tgcttaaata attagaggag gttgttcttt
     6541 taaaatttaa gaaattccgg acagccgaag tgtccactaa ttagaccata gaccatagaa
     6601 agttgtcatt tgtattagcg gatatctcgg ctgttccgga aagggaatgg ccaaaaggag
     6661 gtctaagaat ggaagagacg ataagaagta ttggaatgga aagatttttt cttcttgtgt
     6721 taagagattt acttaacaaa gaagaagatc agatgttgtt agtggattgg ctgaatgcag
     6781 cttttatgaa tacttctttg cagaaagaag acctagaatt tttagaaatt attattaatt
     6841 ctttaatgga aggatacttt acagcgggaa aatttttttc agaaaaagca atagatcttg
     6901 aagaaaaaaa gaaactacca aacaaagttg ttttaattga tggacaaaga atagaaacga
     6961 gatggattga caagaaagaa gaagagttaa tgaaagtgtt agaaattatt agcgggcaat
     7021 aaatttttta cctctcgctc tatttgtcat agataaataa aggcgagagg tcttttaatt
     7081 tacagtccaa aaggaggaaa attgccttga cctattgaca ataaaaagat aatatgatat
     7141 acttccccag ggaaaacaaa aaaagaaaaa aagtggtata ttatgataaa ttaaaagctg
     7201 gaggcgctct tgtccctcgt cttacccccc cctctcttca ctcgtccttt tttaaaggac
     7261 gagtcagatg agggatagga gcacttccgg cctttaattt aaataaaaac agcccgaaag
     7321 agctgttttt aaattgattt aagggtaatt ttaagggctc aaagaatact tcgcattctt
     7381 ttcaccgaat ttacttttta aatttgaatg tggggacaaa actattatcc tctattttcc
     7441 aacgcttttt ctattgtttc ctcgtcagcg tattcaatct caccgccagt gggcaggccc
     7501 cggccgaggc gcgtaatctt tatactttgt tccctttcag tgattaaggg ttttaaaatc
     7561 tttgaaatat aatccattgt taggtttccg ccagcagttg gatttaaggc taaaacaatc
     7621 tcttttggtt taaactcttc aattcttttt tttaattctt caattcgcat tttacccaag
     7681 ggattttctt ttaaaaattc taattctccg cctaaaatga aataacggcc attgtatttt
     7741 ttcatttttt ccatttgcca aaggtcggtt tctttaccta ccacacagat aatttcttct
     7801 ctttttttat caagacatat aggacagagg gtttctttgt tgcgggggga aaaaggatta
     7861 aaacaaaaag aacaaagttt aatttctttt ttaagattca aaatctcctg gcaaattttt
     7921 tccacttttt cttttggcaa agataaaaga tataaagcaa aacgggtggc tgtgcgctca
     7981 ccaattgtag gaaactgaga aaaaatatca acaacctttt tgatttgttt aggaaccata
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atatatttaa acattggaat
     8161 ttaggatttg tttggaattt ggatattaga atttagaatt taatgtttac attatttctt
     8221 cttcgcttct ttcgttctct tcgtcttcat agcttttttc caattctcta aaacttgcta
     8281 agtcctggtt gaaatagaga tcaaccttac caatgggacc attacggtgc tttgcaataa
     8341 tgagttcggt ttttgattct tttatattag ggcggtagat aaagagcact acatcagcgt
     8401 cttgttctaa cgaattatgt acaactatat cattggcaag aaaattatga tagttttcaa
     8461 ccgttgcatc ataaacatcc tctactccta ataattttat tgaaacaatt ttatcccaat
     8521 aaatgtcaga agtagcaagc atttgaatgt taggaatatt aaaaatggca atagaatctc
     8581 caatctttaa ttcatctaat cgtttccatc cttctaattt aaaaaatgga tgattagcgc
     8641 ttgcttttat ttttcgtccg ctttctgttg caagttcaaa aacttctttt ttgccagagt
     8701 aaaaaacttt agtcatttta cttggcctta gcttgttatt gtcatcaaga gcaagcactc
     8761 taattggttc ttgaaatttt ctttgggcaa gttctttcat tgaaactctt tcgccgctat
     8821 ccgctttttg aataagggta tcgccagtta gacagccgct ttcacgaaga tcagagagct
     8881 ttggtctttg atcgggccgg tgttctactg ctcgggaaag ttgtgataaa gctaaaactg
     8941 gtacatttaa ttcttttgct aatgatttta aacttcttga gatttctgtt acttgttgaa
     9001 caggattttc gctttggctt cgtgattcaa tcaactgtaa ataatcaata atgataagtc
     9061 ctagtccttt ttcggcttga agccgtcgag acatcgcccg catttgcaat acagtagaag
     9121 aagcagcatc atcaatataa ataggggctt gagagagggc agaaagggca tcttggatct
     9181 taacgaaatc ctcatctgct gataattttc ctgttcttaa tttccataat gaaatgcctg
     9241 cctgggaagc aatcaatcgg tctaccaatt gttcttttga catttcaaga gaaaacattc
     9301 ccacggtttg gttttcttta agagcagcgg cccgggcaat atcaagggcc aaactggttt
     9361 ttcccaaact tggtcgagaa gccaataaaa taaaatctcc tctttgcagt cctgccaaca
     9421 tatcatcaag ttttggaaaa cccgttgtaa caccccgtaa ggcgcctttt tgagaatgaa
     9481 ttttatcgat tcgttcaaag gcaccatcta attctgtttt tatcggtaaa aagctttgag
     9541 ttaatgactc ttgggtaatg ccaaagattt ttttctctgc ttcatctaat attaaatcaa
     9601 tatctttttc ctcttggtat cccaaagtag agatttcttc agatacttgg atcaagcctc
     9661 ggcgcacttt tttctctcta acaattttgg catagtacat aatgtgcgag ggagtgggca
     9721 cactagaaag gatttctgag agataagccg caccgcctat ctcttctaat tcttcttttt
     9781 cctttaaacg ttgggtaaga gttaaaagat caatgggttc gtttttttca aagatgtcaa
     9841 gcattgcctg ataaatttta ccatggttgt ttttataaaa atcctctggc cttacaaggt
     9901 cgataacttt gatcatgcct tctttgtcca tcattagaga tcccagcagg gctttttctg
     9961 cctcaatgcc ttgaggcgga atttttataa catctgccat aatagaaata cgaaatcaaa
    10021 actataattg ggttcaaaaa tttaacgcaa cactctttaa ttaatttcta atttctaatt
    10081 tctaa
//
LOCUS       DAOTQD010000017         9994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_6281_length_9994_cov_7.063789, whole genome shotgun sequence.
ACCESSION   DAOTQD010000017 DAOTQD010000000
VERSION     DAOTQD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_9994_cov_7.063789"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1360)
                     /gene="ftsA"
                     /locus_tag="P5570_01295"
     CDS             complement(137..1360)
                     /gene="ftsA"
                     /locus_tag="P5570_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRR39378.1"
                     /translation="MAKGNYIFGLDVGSVNLRCVVGEILPNLPLQIVGANISEARGIR
                     KGQVIDIDDASDAIEKNLEEIERRIGERVENVIVSIGGPHIQSINSRGVVAISRADGE
                     VSEDDVSRVLKAAEAISLPKNKEVIHILPREFIVDKERGIKDPIGMHGVRLEVEAEVI
                     FGSTPYINNLIKAIEETGRSFNDVVFSTLAASEAVLSKRQKELGVAVLDIGGGSTGLS
                     VFEEESLLSSSVLPIGASHITNDIAIAFQLPIDIAEKLKLKYGIAKNDNISKKEEIKL
                     EEIGGEEGKVISRKELVEVIEARNLEILELVTKELKRIQRERLLPAGVVLVGGGAKMP
                     GMEELVKRELHLPCQIGFPKDVEGIVDKIDDPSFATAVGLLLFKLKGEKNIPGKAKIQ
                     KGFSRIKKWFEELLP"
     gene            complement(1434..2117)
                     /locus_tag="P5570_01300"
     CDS             complement(1434..2117)
                     /locus_tag="P5570_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRR39379.1"
                     /translation="MLSLTVESRSEKSSLLRKKNLLPGVVYGKDVGNLLIKLPYQEFE
                     KIYKEGGESTIINLKYQTEKGNAKEFPVLIREIQKDPLSNKFIHVDFYQLPMEKEVEV
                     TVPLEFEGKAPAEYELGGVLIKNIREINIRALPKNLITSVRVDVSGLKTFEDEIKIKD
                     LVVPNGVKILANPEEIVALVGKAEEEKIEEISKEEITPEEPELIKKERKEKEGEETEE
                     EKGEGEKEN"
     assembly_gap    2181..2280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2301..3164)
                     /locus_tag="P5570_01305"
     CDS             complement(2301..3164)
                     /locus_tag="P5570_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRR39380.1"
                     /translation="MTNRNQLLKIFKKAQKENYPIFQFNFSTVEQLKGIIEGVKKFNC
                     PIILGTSEGESSFLSLEVAVEMKKIAQKALGKNKVFLNFDHGKSFDIISQAIKAGYDM
                     VHFDGGNLDLQENIKITKKIVEYGKKRDVVVEGEVGFIEQSSVIHKGKPKIKLENLTD
                     PDDAKLFVQKTRVDSLAVSIGNVHGVYQRAENFKLDLERLSVIKREVGDDVFLVLHGG
                     SGISFNQLKKSIEKGIVKININTEIRTAWRKALEKSLKNSPNEITPYKLMSSVTEAIQ
                     KTIEKKLKLFY"
     gene            complement(3170..3712)
                     /gene="lepB"
                     /locus_tag="P5570_01310"
     CDS             complement(3170..3712)
                     /gene="lepB"
                     /locus_tag="P5570_01310"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRR39381.1"
                     /translation="MRNFKQIIIEALKIFVLIAAIVIPIRYFLFQPFFVSGASMEPNF
                     YSGEYLLLDEISYRFRDPLRGEVVVFKYPEDPSLFFIKRVVGLPGETIEISDGIVKIY
                     NDQFPQGKILDEPYVVGKTEGSLRISLKDDEYFVLGDNRYRSSDSRNWGPVSRKNIIG
                     RAWIAISTSKGFEILNAPAY"
     gene            complement(3764..>4238)
                     /gene="rpsI"
                     /locus_tag="P5570_01315"
     CDS             complement(3764..>4238)
                     /gene="rpsI"
                     /locus_tag="P5570_01315"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRR39382.1"
                     /translation="QKKSVAKVNVPQKEEKEKEEKKVLKSDYIQTIGRRKSAICQLRL
                     IKDSKEKKFLINGKDAKKYFPDFENQKNVFAPLQKTNLTATKEIYIEAKVKGGGKKSQ
                     SEAVRLALSRALVEMNSDLREILKAQGYLTCDARVKERKKFGFKKARKAPQWSKR"
     assembly_gap    4239..4338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4358..4723)
                     /gene="rplM"
                     /locus_tag="P5570_01320"
     CDS             complement(4358..4723)
                     /gene="rplM"
                     /locus_tag="P5570_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRR39383.1"
                     /translation="MKIDATNKTLGRLASEIAIILQGKNKASFKAYKVTDEIVEVENV
                     DKMKITGKKMNQKVYYHYTGYPGGIKKITLGDLFKKNPGLVLEKAVWGMLPKNKLRKE
                     RIKRLKIEDKKENIEDNKK"
     gene            complement(4791..5141)
                     /gene="rplQ"
                     /locus_tag="P5570_01325"
     CDS             complement(4791..5141)
                     /gene="rplQ"
                     /locus_tag="P5570_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033017237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRR39384.1"
                     /translation="MKHQKKGRKFHRENGQRNALLKNMGESLILNEKIVTTESRAKDL
                     RVFIEKKISIAKKGGIQSVRELRRYFSEDASQKLIKEIAPALKDRKGGYTRITKIGPR
                     KNDSAEMVQIEIIK"
     gene            complement(5275..6216)
                     /locus_tag="P5570_01330"
     CDS             complement(5275..6216)
                     /locus_tag="P5570_01330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRR39385.1"
                     /translation="MVTLPTSLKILEKTENRLLFEVEPLFPGYGTTIGNALRRVLLSS
                     IEGTAITKVKIKGVSQEFSTLPGLKEDILELTLNLKQIRLKVFGDEPQKIELKVSGKK
                     DVKAGDIKTPSQVEIVNKDLYIASLTSPKSKLEMEMEVQKGFGFVLAEKLQGEKSKVG
                     VIYLDAIFTPIKKVAFWVENIRFEKRTDFNKLKMEIESDGTILPEEALRQAVEILVQH
                     FKCIKDFLPSQEEDNKKKDKNKISSGSSAVENKIISLEDLGLSTRIVKILKENSIKSV
                     AGLLRKKEESLREIEGLGPKAIEEIKKELKKKGLSLN"
     gene            complement(6282..6911)
                     /gene="rpsD"
                     /locus_tag="P5570_01335"
     CDS             complement(6282..6911)
                     /gene="rpsD"
                     /locus_tag="P5570_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRR39386.1"
                     /translation="MQDAKCKICRRSGEKLFLKGDKCSSPKCALLRKPSPPGASPQKR
                     RRIEVSEYGKEMKEAQKIKKMYGIQDRQLKKIVKDIIEKKGQKDASETLIKELEMMLS
                     NIIFRSQLARSRAEARQLVSHGHFLLNGKKIDIPSAKTKVGDEIKLKEKSKNNSYFKN
                     ILMMVKTENIPSWLSFDKNNFSIKVVREPSIDEIGDKTNVPLILSFYSR"
     gene            complement(6987..7439)
                     /gene="rpsK"
                     /locus_tag="P5570_01340"
     CDS             complement(6987..7439)
                     /gene="rpsK"
                     /locus_tag="P5570_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRR39387.1"
                     /translation="MGKKRVIKPTEEALLKERARVEERTGKEGTVKIKSKLPKARIYI
                     NSTYNNTIITLTDQKGNVVYWTSAGRLGFKGTKKGTPFAATKIAETISQIINKLGVFQ
                     VEVFVKGIGQGREAALRGLANRGVNIVMIKDVTPIPHNGPRPPKRRRV"
     gene            complement(7721..8110)
                     /gene="rpsM"
                     /locus_tag="P5570_01345"
     CDS             complement(7721..8110)
                     /gene="rpsM"
                     /locus_tag="P5570_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRR39388.1"
                     /translation="MPRIAGVNIPENKQILYSLPYIYGIGLSLSKKILKEANIDFEKR
                     AKDLSPEEVDRLKNIVEKNYKIEGELRREEMMNIKRLKEINSWRGQRHSRRLPVRGQR
                     TRCNSRTVRGNVRRTVGSGHKPPPAPK"
     assembly_gap    8168..8267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8268..8384)
                     /gene="rpmJ"
                     /locus_tag="P5570_01350"
     CDS             complement(8268..8384)
                     /gene="rpmJ"
                     /locus_tag="P5570_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRR39389.1"
                     /translation="MKVRSSVKKICKDCQVVKRRGKVYIICKKNPKHKQRQG"
     gene            complement(8537..8746)
                     /gene="infA"
                     /locus_tag="P5570_01355"
     CDS             complement(8537..8746)
                     /gene="infA"
                     /locus_tag="P5570_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRR39390.1"
                     /translation="MQKKIREEGKVIESLPNTTFRVKISGDREILAHLSGKMRLNFIR
                     ILPGDKVTVEISEDGKRGRIVYRKK"
     gene            complement(8786..8870)
                     /locus_tag="P5570_01360"
     tRNA            complement(8786..8870)
                     /locus_tag="P5570_01360"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8834..8836),aa:Tyr,seq:gta)
     gene            complement(8963..9034)
                     /locus_tag="P5570_01365"
     tRNA            complement(8963..9034)
                     /locus_tag="P5570_01365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9000..9002),aa:Thr,seq:tgt)
     gene            complement(9146..9481)
                     /locus_tag="P5570_01370"
     CDS             complement(9146..9481)
                     /locus_tag="P5570_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39391.1"
                     /translation="MNTIFICQLILIVAFVGIVFIFLRNFPLLVEFEPRPVPKEKRIS
                     YKIKNNWNNFQKTIGNNFSHWQEKTLRRTKVWMLKIDNSITSHLDSIRKQKIHFSKKQ
                     KESKSNKKK"
     gene            complement(9524..9976)
                     /gene="tsf"
                     /locus_tag="P5570_01375"
     CDS             complement(9524..9976)
                     /gene="tsf"
                     /locus_tag="P5570_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRR39392.1"
                     /translation="MAINVDQIKKLRQLTNISVQECKKALEEAEGDFEKAQKLLKERG
                     AEMIEKKKERETKEGIIGVYLHQNQKVGAMIKISCESDFVARNKIFQDLVHDLAMQVA
                     AMDPKDIDELLEQVYIKDIKKKIKDLIEEAIVKLGENIKIEEIKRMEI"
BASE COUNT         2850 a         1850 c         1153 g         3841 t
ORIGIN      
        1 tacggtagta attcttcgat ctcctgtgaa gggtgagaag aactatggta atagttcttc
       61 gatctccttt caaagggtga gaagaactat ggtaatagct cttcgaactc ctgtaaaggg
      121 tgagaagaac tatgaactac ggtaatagtt cttcgaacca ctttttaatt cttgaaaatc
      181 ctttttgaat ctttgccttt ccaggaatgt ttttttctcc ttttaattta aataataaca
      241 accccacggc agtagcaaaa gaaggatcat caatcttatc cacaatacct tcaacatctt
      301 taggaaatcc tatttggcaa ggaagatgaa gttctctttt tactaattct tccatccctg
      361 gcatttttgc accaccacca accaaaacca cgcctgctgg caataatctt tctctttgaa
      421 ttctttttaa ttcttttgtt actaattcta aaatttcaag atttcttgct tcaattactt
      481 ctactaattc ttttctggaa attacttttc cttcttcacc gccaatttct tctagcttta
      541 tttcctcttt tttagaaata ttatcatttt tggcaatacc gtattttaat tttaattttt
      601 cagcaatatc aatgggtagt tgaaaagcaa tcgcaatatc gttagtaata tgagaagcgc
      661 caatcggcaa aaccgaagaa gaaagtaaac tttcctcttc aaacacagaa agtcctgttg
      721 atccgccacc aatatcaaga actgccactc ctaattcttt ttgacgtttg gaaagcactg
      781 cctcagaggc cgctaaagtt gaaaaaacca catcattaaa agatcttcct gtttcttcaa
      841 tggctttgat taaattattg atatagggag ttgagccaaa aatcacttct gcttcaacct
      901 ctaatcgaac accatgcatc ccaataggat cttttattcc tctttccttg tcaacaataa
      961 attctcttgg taagatatga attacttctt tatttttagg caatgaaata gcttcagctg
     1021 ccttaagcac tcttgaaaca tcgtcttctg acacctcgcc atcagccctt gaaattgcaa
     1081 caacaccgcg agaatttata gattgaatgt ggggaccacc aatactgaca attacatttt
     1141 caactctttc tccaattctt ctttctatct cttcaagatt cttttcaata gcatctgatg
     1201 catcatctat atcaataact tgaccctttc ttattccccg tgcttcacta atgttagctc
     1261 caacgatttg aaggggtaaa ttgggcaaaa tttctcccac tacacatctt aaattaacag
     1321 atccaacatc taaaccaaat atatagttac cttttgccat aaaaattata aaacctaaac
     1381 cctaattata gtttagagtt aaaaaaactt tgaattttta aattaaatct acattagttt
     1441 tctttctcgc cttctccttt ttcttcttct gtctcttcac cttctttttc ttttctttct
     1501 ttcttaatta attctggttc ttcaggtgtt atttcttctt ttgatatttc ttcaattttt
     1561 tcttcttctg ctttaccaac taatgctact atttcttctg gattagctaa aatcttaaca
     1621 ccattaggaa ctaccaaatc ttttattttt atttcatctt caaatgtttt taaaccagaa
     1681 acatcaaccc taactgaagt aataagattt ttgggtaatg ccctaatatt aatttcacga
     1741 atatttttta ttaaaacccc gcctaattca tattccgcag gagcttttcc ttcgaattcc
     1801 aaaggcactg ttacttctac ttctttttcc attggcaatt gataaaaatc tacatgaata
     1861 aatttattgg aaagcggatc tttttgaatt tctctaatca aaacaggaaa ctcctttgca
     1921 ttgccctttt cggtttgata ctttaaatta ataatagtac tttctcctcc ctctttataa
     1981 attttttcaa attcttgata gggtaacttt attaataaat ttccaacatc tttgccatag
     2041 acaacgcccg gaagcaaatt ctttttcctt aataatgatg atttttctga acgagattca
     2101 acggttaaag acaacataaa aataaatttc taattagcta atttttaatt tctaaaaatg
     2161 aattttaaaa tttgaaattt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 tttaaaattt gaaattttta ttaatagaat aatttaagtt ttttttctat tgttttttgg
     2341 atcgcttctg ttacgctact catcaatttg taaggagtta tctcgttagg agaatttttt
     2401 aaactttttt ccaatgcctt tctccatgct gttcgaatct ctgtattaat attaatctta
     2461 acaatcccct tttctatact ttttttcaat tggttaaaag aaatccctga tcccccgtga
     2521 agcactaaaa atacatcatc tcctacttct ctttttatca cagaaagccg ctcaaggtca
     2581 agtttaaaat tttctgccct ctgataaaca ccatgaacat taccaataga cacagctaaa
     2641 gaatcaaccc ttgtcttttg aacaaaaagt ttagcatcat caggatcagt taagttttct
     2701 aattttattt taggttttcc tttatgtata accgatgact gctcaataaa acctacttca
     2761 ccttccacta caacatctct ttttttaccg tactcaacaa tttttttggt aattttaata
     2821 ttctcttgaa gatcaagatt tcctccatca aaatgcacca tatcataccc tgctttgatt
     2881 gcttgagaaa ttatatcaaa ggacttacca tgatcaaaat tcaaaaacac cttgttcttt
     2941 ccaagcgctt tttgagctat ttttttcatc tccactgcta cctccaagct caaaaaacta
     3001 ctttctccct cggaagttcc caaaataata ggacaattaa acttttttac cccttcaata
     3061 atccctttaa gttgttccac tgttgaaaaa ttaaattgaa aaatgggata attttctttt
     3121 tgggcttttt taaaaatttt taataattga tttcggtttg tcattttaat taatatgctg
     3181 gtgcatttaa aatttcaaat ccctttgatg tagaaattgc aatccaggct ctaccaataa
     3241 tattttttct tgaaacgggt ccccaatttc tagaatctga agaacggtat cgattatccc
     3301 ccaaaacaaa atattcatca tcttttaaag aaatccgcaa actgccttct gttttgccaa
     3361 caacatatgg ttcatctaag attttgccct gaggaaattg atcgttgtaa atctttacta
     3421 ttccatcgct aatttctatt gtttcgccag gcaaccccac tactctttta ataaaaaaaa
     3481 gtgaaggatc ttctggatac ttaaaaacta ccacttctcc ccgcaagggg tctctaaatc
     3541 tatatgaaat ttcatctagt aaaagatatt cccctgaata aaaattgggc tccattgagg
     3601 caccacttac aaaaaatggt tgaaataaaa aatatcgaat aggaattaca atcgctgcta
     3661 ttaaaacaaa tattttcaag gcctcaataa ttatttgttt aaaatttctc ataaatatat
     3721 aatataattc caatctccat tgaagggctt acattttttt ggactatctc ttagaccact
     3781 gaggagcttt tcttgctttt ttaaatccaa atttttttct ttcttttact cgcgcatcac
     3841 aagtaagata cccttgggcc tttaaaattt ctctaaggtc tgaattcatt tcaactaatg
     3901 cccgggacaa agctaatctt actgcttcgc tttgagattt tttcccacct ccttttactt
     3961 tcgcctcaat gtaaatttct tttgtagcgg taagatttgt tttttgtaaa ggagcaaaaa
     4021 catttttttg attctcaaaa tctggaaaat atttcttagc atcctttccg ttaatcaaaa
     4081 attttttttc tttactgtcc tttatcaatc ttaattgaca aattgcactt ttacgacgac
     4141 cgatggtttg aatataatct gatttcaaaa cctttttctc ttccttttcc ttttcctctt
     4201 tttgaggaac attaactttt gcaacagatt tcttctgcnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnntc gctgtttttt tcatagatta ttttttatta tcttctatgt
     4381 tctctttttt gtcctctatt tttaatctct taattctttc ttttcttaat ttatttttag
     4441 gaagcatgcc ccaaactgct ttttctaaaa ccaaaccagg atttttttta aaaagatctc
     4501 ccagggtaat ttttttaatg ccacctggat aaccagtata atgataataa actttttgat
     4561 tcattttttt gccagtaatc ttcattttat ctacattttc tacttcaaca atctcatcag
     4621 taaccttata ggccttaaaa cttgccttat ttttaccttg aaggattatg gcaatttcac
     4681 tggctaatct tcctaatgtt ttatttgtag cgtcaatttt cataaaatta gagagttgag
     4741 agtagaaagt tgataattgt aactactttc tacattctac attctacctt ctattttatt
     4801 atttctattt gcaccatttc tgcgctatca ttttttcttg gaccaatttt tgtaattctg
     4861 gtatatcctc cttttctatc cttaagggct ggtgcaattt cttttattaa tttttgagaa
     4921 gcgtcttcag aaaaatatct tcttaattct cttactgatt gaattcctcc ttttttagct
     4981 atagaaattt tcttttcaat aaaaactctc aaatctttcg cacgagattc agtggtaaca
     5041 attttttcat tcaaaatcaa actttcaccc atattcttta aaagagcgtt tcgttgacca
     5101 ttttctctat gaaattttcg accctttttt tgatgtttca tatatttggt ttattgaaat
     5161 taagcttatt agaaaaatca atgcattacg aacctgttac gaatttacaa atgtaaattt
     5221 aaaaataatc caaaggcgca ttatgatatt cgtatattca cattaattcg taaattaatt
     5281 caagcttaat ccttttttct ttaactcttt ttttatttct tcaattgctt tggggcccaa
     5341 cccttctatc tctcttaaac tttcttcctt cttacgtaaa agcccagcta cgcttttaat
     5401 actgttttcc tttaaaattt ttacaattct tgtagaaaga cctaggtcct caagagaaat
     5461 aattttattt tccactgcag atgacccact cgaaatttta tttttgtctt tctttttgtt
     5521 gtcttcctct tgagaaggta aaaaatcttt aatacattta aagtgttgaa caaggatttc
     5581 tactgcttgc cttaatgctt cttctggcaa aattgttcca tcgctttcaa tttccatttt
     5641 tagtttatta aaatctgttc ttttttcaaa tctaatattt tccacccaaa aagccacctt
     5701 tttaataggt gtaaaaatgg catctaaata aattacaccc actttagatt tttcaccctg
     5761 aagcttttca gcaagtacaa atccaaagcc tttttgaact tccatctcca tttccaattt
     5821 tgattttgga gaagtaaggg aagcaatata taaatcctta ttaacaattt caacctgaga
     5881 cggagtttta atatctcctg ctttcacatc tttttttccg ctcaccttta attcaatttt
     5941 ttgtggttca tctccaaaaa cttttaacct tatttgttta agatttaatg taagttcaag
     6001 aatatcttct ttcaatccag gcaatgtact aaattcctga gatactcctt taattttaac
     6061 tttagtaata gctgtaccct cgatcgaaga aagcaatact cgacgcaggg catttcctat
     6121 tgtagttcca tatcctggaa aaagcggttc tacttcaaaa agaagtcgat tttcagtttt
     6181 ttctaaaatt ttgagtgatg ttggtaaagt aaccataagt atttagtatt ttagtataaa
     6241 cacacatata tgctactatg ctagcatatg atcatatttt attatcttga gtaaaatgac
     6301 aaaataagag gtacatttgt tttatctcct atttcatcga tactcggctc acgaacgacc
     6361 tttattgaaa aattattttt atcaaaagaa agccaactag gaatattttc tgttttaacc
     6421 atcattagaa tgtttttaaa ataagaatta ttctttgatt tttcttttaa ttttatttcg
     6481 tctcctacct ttgtttttgc tgaaggaata tctatttttt ttccatttaa aagaaaatga
     6541 ccatgagaaa ccaattgtct tgcttctgct ctagatcgtg ccaattgtga tctaaaaata
     6601 atattagaaa gcatcatctc tagttcttta attagcgttt ctgatgcatc tttctgtccc
     6661 tttttttcta ttatatcttt tacaatcttt ttaagttgac gatcttgaat accatacatc
     6721 tttttaattt tttgtgcttc cttcatttct tttccatatt ctgaaacctc aattcttctt
     6781 ctcttttgag gcgatgcacc aggaggagat ggttttcgta ataaagcaca ctttggagat
     6841 gaacatttat ctccttttag aaaaagcttc tcaccacttc ttcgacaaat tttacattta
     6901 gcgtcttgca taaaatattg gagtaattgc gaatatacga attttaaact aatatacgaa
     6961 tttgtatatt agtgagtatt cggatattat actcttcttc gttttggtgg tcttggacca
     7021 ttatggggaa taggcgttac atcttttatc attacaatat taacaccacg attagctagc
     7081 ccccttagag ccgcttctcg tccttgccct atacctttta caaaaacttc tacttgaaaa
     7141 actcctaatt tgttaataat ttgacttatt gtttctgcta ttttagtagc tgcaaaggga
     7201 gttccttttt tcgtgccttt aaaacccagc cgtccagcag aagtccaata aacaacatta
     7261 cctttctgat cagttaatgt aataatagtg ttattataag tagagttaat ataaatacgg
     7321 gctttaggaa gcttactttt tattttcact gttccctctt taccagttct ctcttcaacc
     7381 cttgctcttt ctttcaaaag agcctcttct gttggtttta taactcgttt tttacccata
     7441 atagtcgcct ctaaagaggc tataatttct aatttctaat ttctaatttc taatttctaa
     7501 acctaccctt aaccaccctt aaattcgtat attcgtacag attcgtaaat tcgttgattg
     7561 atattcgtag attcggataa taaattaaat ttgtgatttg gtatttattt taatgtgttg
     7621 tgttaaaaac ttgaatctgc cttatattta acttagagtt tcgatcagtt gaatttattt
     7681 ctttatttcg gatttataat tttgttactt agaattttta ttactttggt gcgggtggtg
     7741 gtttatgacc actgccaact gttcttctta cattgcctcg aacagtcctt gaattacatc
     7801 tggttctttg tcctcttacc ggtaatcttc ttgaatgtct ttgcccgcgc caactattaa
     7861 tttcttttaa tcttttaata ttcatcattt cctcgcgtcg caattctcct tcaattttat
     7921 aatttttttc aacaatattt ttcaaccgat ctacttcttc tggagataaa tcttttgccc
     7981 tcttttcaaa atcaatattt gcttctttta atattttttt tgacaaagat aaaccaattc
     8041 cataaatata tggaagcgaa tataaaattt gtttattttc tggaatatta actcctgcaa
     8101 tacgaggcat aaataaattt ttaattttta atttttaatt tttaaacctt actgtttgaa
     8161 aattgaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta cccttgtctt
     8281 tgtttatgtt ttgggttttt tttacaaata atatacacct ttcctcttct ttttacaacc
     8341 tgacaatcct tgcaaatttt ttttacagat gatcttactt tcattttatt aaactctcga
     8401 atttttcctt aaattattac gaatatttct cgaatattct aaccctaaga atattttata
     8461 ttctttttac caaatttaca aagtaaattc gagattaatt cgatttaatt tgtggaatat
     8521 ttaaaaaaga tattttttat tttttcctat acactatcct gcctctttta ccatcttctg
     8581 agatttcaac agttacttta tcgcccggca aaattctaat aaaatttagc cgcatcttac
     8641 cggacagatg ggctaaaatt tctctatccc cggaaatctt tactctaaaa gtggtattgg
     8701 gcaaagattc aattactttg ccttcttctc taattttctt ttgcatttat tattttagca
     8761 aaaatttaaa aaaaagcaag ttatttgggc cgggtaggat tcgaacctac gtagccatta
     8821 aaggcgcgtg ttttacagac acgagcattt gaccgctctg ccaccgaccc tcttttttct
     8881 attttacaaa aaatgaatta tttaataaat taatttaatt ataacaatta aaaacagcaa
     8941 aatcttactc gccgtaaatt ttagccgtcc atcggatttg aaccgataac ctactgttta
     9001 caaaacagtt gctctgccat tgagctagga cggcgagtaa atttaagaaa tgaatcgctg
     9061 agaacagcaa taacttttaa ttctaaatat acaatttctt cctctctcta attttgtttc
     9121 aattaatcct agatttagaa aatttttatt tctttttatt actcttagat tctttttgtt
     9181 ttttagaaaa atgtattttt tgtttcctta tgctgtcaag gtgcgaagtg atagaattat
     9241 caattttaag catccaaact tttgtcctgc gaagagtttt ttcttgccaa tggctaaaat
     9301 tatttccaat tgttttttga aaattattcc aattgttttt aatcttataa gatattcttt
     9361 tttcctttgg aacaggcctt ggttcaaact ctaccaataa tggaaaattt ctcaaaaaaa
     9421 taaaaacaat tcccacaaat gctacaatta aaatcaattg acaaataaag atagtattca
     9481 tttaagtatt tttatactaa aatacttcta tgtttaacat agattatatt tccattcttt
     9541 taatttcttc tattttaata ttttctccta attttactat tgcttcttca attaaatcct
     9601 ttatcttttt ctttatatct ttaatataca cttgttctaa taattcatca atgtctttgg
     9661 ggtccattgc tgcaacttgc attgccaaat catgaaccaa gtcctgaaaa attttattcc
     9721 ttgccacaaa atcactttca caagaaatct ttatcatcgc ccctaccttt tgattttgat
     9781 gtagataaac gccaataatc ccctcttttg tctcacgttc ttttttcttt tcaatcattt
     9841 cagctccccg ctcttttaat aatttctgag ctttttcaaa atctccctct gcttcttcaa
     9901 gggctttttt acattcttga acagaaatat ttgttaactg ccgaagtttt ttgatttgat
     9961 caacattaat agccataatt aaaaaattta aatt
//
LOCUS       DAOTQD010000018         9299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_6790_length_9299_cov_8.638035, whole genome shotgun sequence.
ACCESSION   DAOTQD010000018 DAOTQD010000000
VERSION     DAOTQD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6790_length_9299_cov_8.638035"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1532
                     /locus_tag="P5570_01380"
     CDS             6..1532
                     /locus_tag="P5570_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR39393.1"
                     /translation="MSFPKEVLFIIKTLEEAHFEAYLVGGCVRDLLRNLEPKDWDIAT
                     NATPKEIQQLFPRSFYDNKFFTVKVATESTDPRLKEIEITTFRSEAKYTDRRHPDEIK
                     PAKTIEEDLSRRDFTVNAMAMKLTVEQPVKNIKVVNYKIIDLFKGKEDLKNKIIRAVG
                     EPKERFAEDALRMIRAVRFAATLNNDQFSLSQQKTDSLSKLEWQIESNTLQAIKRNAS
                     LLEFVSKERIRDELIKILESPYAAEGILLLHQTGLLKYIIPELEKGVGITQNKHHIYT
                     IFKHSLMALKYAAKYHYNLNVRLAALLHDVAKPQTKRGEGLDATFYNHDVVGAAITQK
                     ILSRLKFPKKTIKKVTTLVRYHMFFYDPKTVTESSVRRLLKKVGTENIQELIQLRICD
                     RKGMGRPKAKPYKLRYLEYIISRVSKDPISVKMLKVDGQDVMKILNIKSGPKVGLILN
                     ALLSEVIDDPQKNNKKYLEKRIKELGRWSEEELREKEKRIEEKKMEIETEEKQKFHIK
                     "
     gene            1545..2108
                     /locus_tag="P5570_01385"
     CDS             1545..2108
                     /locus_tag="P5570_01385"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRR39394.1"
                     /translation="MLNPNELKIGDIILYNKELFEILEIKHLHLGRGGAVFQTKIRNL
                     KTNNVFSQNFKPGDRLEEADIEEEVIRYLYNHRGEYFFCKKENPSERFSLNEEMIGKY
                     KFFLKSGIDIEAIKFQNKIINIILPIKIDLKVTEAPPNFSGNTVDRGTKKITLETGYE
                     VSAPLFIKEGDIVRINTKTGEYVERIK"
     gene            2199..2738
                     /locus_tag="P5570_01390"
     CDS             2199..2738
                     /locus_tag="P5570_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39395.1"
                     /translation="MKLPLTDEFLLKIYNYLEKVMELDNAPHSFQEAAVTDFIWMRKN
                     YDKARRKKQFSHFLSYLKKKGYIKVKQLEAGKAVFLTQKGKEKALKTKLKFLEKDFQS
                     SKLKKRKDGKLLMVIFDIPEDKRDWRNVLRDGLMSLGYTFFQKSVWISPFDVYQKTQQ
                     LILECGLDKYCHIFLIEKI"
     gene            2791..3843
                     /gene="rsmA"
                     /locus_tag="P5570_01395"
     CDS             2791..3843
                     /gene="rsmA"
                     /locus_tag="P5570_01395"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRR39396.1"
                     /translation="MHKGKTDIGENKKGNKKGNKRGDEGGNKKENERGNKNRSENLKS
                     LINECNFEPKKHWGQNFLMDKEVLADIVKTADLKKSDVVLEIGPGFGVLTKELAKKTS
                     KVIAVEKDERLIKMLKRGLKGFKNVEIIHGDILKINIKNVIASEGAKRPSAAISDDNY
                     NNTTGLLYPTSGRTRNDKMYYKVVANLPYYITSPVIRKFLEEKNPPKLMVLMVQKEVA
                     ERICAKPPKMSLLSVACQFYATPEIVRIVKKESFWPKPKVDSAIIKLKINNLQLATEF
                     REQFFKIVKAGFSSPRKQLKNNLKRKLKLDEKTVEDWLFKNNIQPIQRSETLLVEDWI
                     NLTNSFPLSTPLQAPQ"
     gene            complement(3818..5215)
                     /locus_tag="P5570_01400"
     CDS             complement(3818..5215)
                     /locus_tag="P5570_01400"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRR39397.1"
                     /translation="MFKKQKYFVFILFLLLPLFSLFADEVDYPDLPNVPAPTPEQGLP
                     SYVNYIFNIALALGGLVAFGILIWGGVLYLTSAGNPEQVGSAKKKLTSALSGLLILFA
                     IYLILTTINPDLAVTGISFFQDISILQPTVGICLCPSTSPCTRTCDGENNFFITDSRK
                     VLDLPDKFKNEKWEVKFYTENPLIYIFDREGYQEGKWTSRKKLESGESIDFFPKSVYF
                     AKPNGVCLYDHVVTDEKNLMHFPEHFVCLTDSAPNLGLVEPFLNDKAQAVIIYGDYGA
                     ILFDKESYNGNYAMVCAGENAINVGDLGKAGEEDCWGVKGLVSNNPPIGNSTVSSVEV
                     WRDNREKNISGSITFYNRPDCKGGEYKVPNGEVSVPGVVEINDDSDQIKVFSDGKKVF
                     ENIVSIKINGTFAVILEDQPDSTGDAQLFKKTAINNCIPSLVGSAVFESTPESPNPGY
                     QTKKIRRIIVVPARE"
     gene            complement(5217..6572)
                     /locus_tag="P5570_01405"
     CDS             complement(5217..6572)
                     /locus_tag="P5570_01405"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRR39398.1"
                     /translation="MNKKTKIITILLCGLLLLPLFSLFADEVDYPDLPNVPAPTPEQG
                     LPSYVNYIFNIALALGGLVAFGVLIWGGVLYLTSAGNPEQIGSAKKKLTSAISGLLIL
                     LCVYLILTTINPDLALLSLPTISPTSGVWLVKSNGEKIYFSDNASPVSLTGISGIEFI
                     SSRDKLMGVYLYTKSNYLGGEEWVETPENIENSVSVNTSNVKSIYFLWRKPGVYLYPE
                     TNFQGRPLYVKDSVSDLGKIKSSGYNGFDNKTSSVRIINELPIRYGVVLFEMPGFYSE
                     NNCPDIYSVSKEILEIPNLSISTPVADRSYNNPPIGNEKVSSLVVFYYNWSNPSSGKI
                     TFFDKTGCSQATGSPLGINIDPYDVKKGRFSDYSYGSGSESWDGKVISFMISGSAAVI
                     LKTGDIWEGKEDGFCMYFSRDRGGFEENGCYSKLHVLTSIFDPSAGIYPRKYVILPIR
                     D"
     gene            complement(6565..>9299)
                     /locus_tag="P5570_01410"
     CDS             complement(6565..>9299)
                     /locus_tag="P5570_01410"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRR39399.1"
                     /translation="FADEVDYPDLPNVPAPTPEQGLPSYVNYIFNIALALGGLVAFGV
                     LIWGGVLYLTSAGNPEQIGSAKKKLTSALSGLLILFAIYLILTTINPDLVSLQLPEII
                     GTPPSGPLTPTEPQKLELVFKEIPLGTIAESILAKNISCFDKDRNLIDCQQSECIIDN
                     QHAGQYICQKGQVLQGAVENIFSSETDFRYCYQYDENGNKEKLLENNDQYQCLQNLID
                     AIEIKTQKLIDLSNELNSLLKNGCKCSNCSTGWCTTKKSEGCSYCDSHCSCCGGPRGD
                     KSCNPNEGIFKSGYDPCSNRKEIDIKREEIKILIENGDPTDYSNDNYEKEVYNTAKEN
                     LGVNEKFLSLNEAKRRIEGIKNDLENGLNDLKKAENLMKTPFGKRLTLAEYFVFKQQS
                     QIPVQKEEFGNYKIEKYCFYFNCNSTTNKDLCNSCGLSNQKRMCRIETANNISVEDYV
                     FLGDPATFYLIKQCKEYADENSCKRANCNWSEDGCWGDTGQEYLEETLQGKSSLNIEE
                     EKGFIKSLIPIGETVDETEVFVSKILSPVQIIGENLQNVINAGNDLYGFPEQCECSSR
                     CTNSSKCKSCGCCRRCDCDEPPCGCCASDYCCSGCTTCAPRQGGSDYYVCPINDIEAR
                     IKDISKKTSDKDNYIEASSINEQKKESKATFTINLDPNCESYAIQLLDPCDSSLWMRF
                     YKTAVLERDGENLNYQIPAPEGSYSDHSEYWEVNRKTIGWGKYSSCQDPATFYLDLLG
                     EIRWSGSSKLIITLETNYKVGSNYSFQATIKKSCSGYLQSIETAQQRLSNLTEAKNLT
                     KSDPNRCTLLDKLSLSRDRLEKCITGYGFAFKESSKKRIFSSKIGLDLVYLGKWIILP
                     DFPYPPLKGELNAYPYNSSMLTDEQKEICRNNKDSLKCSDYIKDLLENYYCLYE"
BASE COUNT         3243 a         1665 c         1561 g         2830 t
ORIGIN      
        1 tttttatgtc ttttccaaaa gaagtccttt ttatcattaa aaccctggaa gaagctcatt
       61 ttgaggcata tcttgttggc ggctgtgttc gtgatctttt gcggaatctt gaacccaagg
      121 attgggatat tgccactaat gccacgccaa aagaaattca acaacttttt ccccgctctt
      181 tttacgataa taaatttttt acagtaaagg tagcaactga aagtactgat cctcgtctaa
      241 aagaaataga aatcaccacc tttcgctcag aagcaaaata tactgataga cgccacccag
      301 acgaaataaa gccagcaaaa accattgaag aagacctatc gcgtcgtgat tttactgtaa
      361 atgcaatggc aatgaaatta acagttgaac aaccagttaa aaatattaag gttgtaaatt
      421 ataaaattat agatttattt aagggtaaag aggatttaaa aaataagatt attagggcag
      481 taggggaacc aaaagaaaga tttgctgaag atgctctacg tatgatacgg gctgtacggt
      541 ttgcagcaac ccttaacaat gatcaatttt ctctgtctca acaaaaaact gattctttga
      601 gtaaattgga atggcagatt gaatccaata ctcttcaagc aataaaaaga aatgcttctc
      661 tgctagaatt tgtttcaaaa gaaagaatta gagacgagct tattaaaatt ttagaaagcc
      721 cctatgctgc tgaaggaatt ttgcttttgc atcaaacagg actcctaaaa tatatcattc
      781 cggaattaga aaaaggggtg ggcattactc aaaacaagca ccatatttac accattttta
      841 aacattcttt aatggcttta aaatatgcgg caaaatatca ttataatctt aatgtgcgat
      901 tagccgcgct tttacatgat gttgctaaac cccaaacaaa acgtggagaa ggattagatg
      961 ccacctttta caatcacgat gtagtaggag cagcaattac ccaaaaaatc ctttcgcgat
     1021 taaagtttcc taaaaaaact attaaaaagg taacaacact ggtacgatat catatgtttt
     1081 tttatgatcc taaaactgtt actgaatcat cggtaagacg gctgcttaaa aaggtaggga
     1141 cagaaaacat tcaagaattg attcaattaa gaatttgtga tagaaaagga atgggcaggc
     1201 ccaaggccaa accctataag ctaagatatt tggaatatat tatctcaagg gtgtcaaagg
     1261 atccaatttc agtaaagatg ctcaaagtag acggtcagga cgtaatgaaa atcttaaata
     1321 tcaagtcagg accaaaggtc ggattaattc tcaatgcctt attatcagag gtgattgatg
     1381 accctcaaaa aaataacaaa aaatacttag aaaaaagaat aaaagaactt ggaagatggt
     1441 ctgaagaaga attaagagaa aaggaaaagc gaatagagga aaagaaaatg gaaatcgaaa
     1501 cagaagaaaa acaaaaattt catataaaat aatttttaaa aattatgtta aatcccaatg
     1561 aattaaaaat aggagatatt attctttata acaaagagtt gtttgaaata ttggaaataa
     1621 aacacttaca cttgggtagg ggaggagcag ttttccaaac aaaaataaga aacctgaaga
     1681 caaacaacgt tttttcgcaa aattttaaac ccggcgacag attggaagaa gcagatattg
     1741 aagaagaagt aattagatat ctttacaacc atagaggaga atatttcttc tgcaaaaaag
     1801 aaaacccttc agaaagattt tctttaaacg aagaaatgat tggcaaatat aaattttttt
     1861 tgaaatccgg cattgatatt gaggcaataa aatttcaaaa taaaattatt aatattatac
     1921 tgcctataaa aatcgattta aaagtaacag aagcacctcc aaactttagt ggcaatactg
     1981 ttgacagagg aacaaaaaaa attactcttg agactggata cgaagtaagc gcaccattat
     2041 ttattaaaga aggggacatt gtgagaataa atacaaaaac tggcgagtac gttgaaagga
     2101 taaagtaaac ccgcctacta ttacttcact attacttatt atgtctacaa atagacgatc
     2161 gtctatttgt agacataatg atttatttta tttttattat gaaattgcca cttactgatg
     2221 aatttctttt aaagatttat aattatttgg aaaaggtgat ggagttagat aatgctcccc
     2281 actcttttca agaagcagcg gtgactgatt ttatctggat gagaaaaaat tatgataaag
     2341 caagaaggaa aaaacaattt tctcactttt taagttatct taaaaagaag ggatatatca
     2401 aagtcaaaca attagaagcc ggcaaagcgg tctttttaac tcaaaaaggc aaagagaagg
     2461 ccctcaaaac aaaattaaaa tttctggaaa aagattttca gagctctaaa ttaaagaaaa
     2521 gaaaagatgg aaaacttttg atggtaattt ttgatattcc cgaagacaaa agagattgga
     2581 gaaacgttct cagagatgga ttaatgagtt tgggatatac attttttcaa aagagcgttt
     2641 ggatttctcc ttttgatgtt tatcaaaaaa cccagcaatt aatattggaa tgcggattag
     2701 ataaatattg ccatattttt ttaatagaaa aaatttaata taaatgtacc tttttagacg
     2761 atcgtctaaa aaggtacatt ggaaaatata atgcataaag gaaaaacaga tataggagag
     2821 aacaaaaaag gaaacaaaaa aggaaacaaa aggggagacg aaggggggaa taaaaaggaa
     2881 aatgaaaggg gaaataaaaa cagaagtgaa aatttaaaaa gcttaattaa tgagtgcaat
     2941 tttgaaccaa aaaaacattg gggacagaac ttcttgatgg ataaggaggt tttggcagat
     3001 attgttaaaa cagcagatct taaaaaaagc gatgttgtgc tagaaattgg gccgggtttt
     3061 ggggttttaa caaaagagtt agcaaaaaaa accagcaagg taattgctgt tgaaaaagat
     3121 gaaagattaa taaaaatgtt aaaaagggga ttaaaagggt ttaaaaatgt tgaaattatc
     3181 cacggcgata ttttaaaaat aaatattaag aacgtcattg caagtgaggg agcaaagcga
     3241 ccaagcgcag caatctcgga cgataactat aataatacta cgggattgct ttaccccact
     3301 tctggcagga ctcgcaatga caaaatgtat tataaggttg ttgcgaactt gccttattat
     3361 attacttcgc cagtgatcag aaagttctta gaagaaaaaa atccgccaaa attaatggtg
     3421 ctaatggttc aaaaagaagt agcagaaaga atttgtgcga aaccgccaaa aatgagttta
     3481 ctctcggttg cttgtcaatt ttatgcaacg ccagaaattg ttagaatagt aaaaaaagag
     3541 agtttttggc ccaaaccaaa agtggattct gcaataataa aacttaaaat taataattta
     3601 caactggcaa ctgaatttcg cgagcagttt tttaaaattg tcaaggcagg attttcttct
     3661 ccccgcaaac aactcaaaaa taatctaaag agaaaattaa aactagatga aaaaacagtt
     3721 gaagattggc tttttaaaaa taatatccag cccatccaac ggtcagagac cttgttagta
     3781 gaagactgga taaaccttac aaacagcttc cccttatcta ctcccttgca ggcaccacaa
     3841 taattcttct tatttttttt gtttgatagc ccggattggg gctttcaggt gttgattcaa
     3901 aaaccgcaga ccccaccaaa cttggaatac aattattgat tgcggttttt ttaaacaatt
     3961 gggcatcgcc tgtactgtct ggttgatcct ctaaaattac tgcaaatgtg ccgttaatct
     4021 ttattgaaac aatattttca aagacctttt ttccatctga aaaaacctta atctgatcag
     4081 agtcatcatt gatctcaacc acgcccggca cagatacttc tccattgggc actttatatt
     4141 cccctccttt gcaatcaggt ctgttataaa atgttatact tcctgatata tttttttctc
     4201 tgttgtctct ccaaacctct actgaagaca ctgtgctgtt gccaatcggt ggattattac
     4261 taaccaaccc ttttactccc cagcaatctt cttctcccgc cttccccaag tcccctacat
     4321 ttatagcatt ttctcccgca cacaccatcg cataattccc attataactt tctttgtcaa
     4381 ataaaattgc tccatagtcg ccgtaaataa tcactgcctg tgctttgtca ttcaaaaatg
     4441 gttccaccaa gcccaggtta ggcgcgctat cagtaaggca tacaaaatgc tctggaaaat
     4501 gcatcaagtt cttttcatca gtaacaacat gatcatacaa acatacccca tttggctttg
     4561 caaaataaac agactttggg aaaaaatcaa ttgattctcc gctctctaac ttttttctgc
     4621 ttgtccattt accttcctga tacccttccc tatcaaaaat ataaatcaat gggttttcag
     4681 tataaaattt tacttcccac ttttcgtttt taaatttatc gggcaaatca agaacttttc
     4741 gactgtcagt aataaaaaaa ttattctctc cgtcacaagt acgggtgcaa ggactagtag
     4801 agggacataa acaaatgccc acagttggtt gaagaatact aatatcttga aaaaaagata
     4861 ttccagtaac agccaaatca gggttaatag tagttaagat tagatagata gcaaagagaa
     4921 ttaagagtcc agaaagggca ctggtaagtt tctttttggc actgccaacc tgctcagggt
     4981 taccggcaga ggtaagatat aatacgcctc cccagattaa aatgccaaaa gccactaacc
     5041 cacccagggc cagagcaata ttaaagatat aatttacata actgggcaaa ccttgttctg
     5101 gggttggggc agggacattg ggcaaatcag gataatctac ctcatcagca aaaagagaaa
     5161 agaggggcag caataaaaat aaaataaaaa cgaaatattt ttgtttttta aacattttaa
     5221 tccctaatag gaagtattac atatttacga ggatatattc ctgcactggg atcgaaaata
     5281 ctagtaagaa catgaagttt tgaataacat ccgttttctt caaatccccc cctgtctcta
     5341 gaaaaataca tacaaaatcc atcttccttt ccttcccaaa tatcaccggt ctttaaaata
     5401 acagctgctg atccactaat cataaaagaa atcacttttc cgtcccaact ttcacttcca
     5461 cttccatagg agtaatcaga aaaacgcccc tttttaacat cataaggatc tatatttatt
     5521 ccaaggggac taccagttgc ctgtgaacat ccagttttat caaaaaaagt gattttgcca
     5581 ctactgggat tgctccaatt ataatagaaa actactaatg aagaaacttt ttcatttcct
     5641 ataggtggat tattataaga cctatccgcc acaggcgtag atatgctaag attgggaatt
     5701 tctaaaattt ctttactgac tgagtagata tcaggacaat tattctcact ataaaaaccc
     5761 ggcatttcaa aaagcactac cccataacga atgggtaact cgttaataat tcgaacagaa
     5821 ctagttttat tatcaaaccc gttgtatcca ctgcttttta tttttcctaa atctgaaact
     5881 gaatctttaa catacagtgg cctcccctga aaatttgttt caggataaag ataaacacct
     5941 ggttttctcc ataaaaaata aatagatttt acattgctgg tattaacaga aacagaattt
     6001 tcaatgtttt ctggtgtttc tacccattct tctccgccca aataattgct tttggtataa
     6061 agataaaccc ccattagctt atctcttgaa gaaataaatt ctattccgct aataccagtt
     6121 aaagaaacag gagaagcatt gtcagaaaaa taaatttttt caccattact ttttaccagc
     6181 cacacaccag atgtgggact gatggtaggc aatgaaagca aagccaaatc aggattaata
     6241 gtagttaaga tcaaataaac gcagagaaga attaagagtc cggaaatggc actggtaagt
     6301 ttctttttgg cactgccaat ctgctcagga ttgccggcag aggtaagata taatacgcct
     6361 ccccagatta aaacaccaaa agccactaat ccacccaggg ccagagcaat attaaagata
     6421 taatttacat aactgggcaa accttgttct ggggttgggg cagggacatt gggcaaatca
     6481 ggataatcta cctcatcagc aaaaagagaa aagagaggca gcaataaaag tccacaaagt
     6541 aaaatggtta taatttttgt ttttttattc atataagcaa taataatttt ctaataggtc
     6601 cttaatataa tccgagcatt ttaaactgtc tttattgttt ctgcaaatct ctttttgctc
     6661 atctgtaagc atgctgctat tataaggata agcattcaat tctcctttta atggcggata
     6721 aggaaaatcc ggcaaaataa tccacttacc caagtatacc aaatccaaac caatttttga
     6781 agaaaaaatt cttttttttg acgattcttt aaaagcaaaa ccatacccag taatacattt
     6841 ttctaatcga tctctggaaa gagaaagctt gtctaaaaga gtgcatcgat taggatcgct
     6901 cttggttaaa ttttttgctt cagttaaatt gcttaatcgc tgttgagcgg tttcaatgga
     6961 ctgaagatat ccagaacagg attttttaat agtggcttga aaactataat tactgccaac
     7021 cttataattg gtctcaaggg ttattatcaa tttagaagaa ccgctccatc taatttcccc
     7081 tagcaaatct aaataaaagg ttgctggatc ttgacaggaa gaatatttgc cccatcctat
     7141 tgtctttcta ttgacctccc aatactctga atgatcagaa taggatcctt ctggcgctgg
     7201 aatttgataa tttaaatttt ctccatctct ctctaaaaca gctgttttat aaaatcgcat
     7261 ccataaagaa ctatcacaag gatctaaaag ttgaatagca taactctcac aatttggatc
     7321 aaggtttatg gtgaatgttg ctttggattc ttttttttgt tcgttgatag aagaagcttc
     7381 aatataatta tccttatcgc ttgttttttt tgaaatatct ttaattcttg cttctatatc
     7441 gttgatagga caaacataat aatcagatcc cccctgtctt ggtgcacatg ttgtgcaacc
     7501 agaacaacaa taatcgctcg cacaacagcc acaaggtggc tcgtcgcaat cgcaacgacg
     7561 acagcaaccg caactcttac actttgaact gtttgtgcat cttgatgagc attcacattg
     7621 ctcaggaaat ccatataaat cattccccgc atttattaca ttttgtaagt tttcaccaat
     7681 aatctgaaca ggggataaaa tcttactgac aaaaacttct gtttcatcaa cggtttctcc
     7741 gatgggaatt aaacttttaa taaatccttt ttcctcttct atgtttagtg agctttttcc
     7801 ttgtaatgtt tcttctaagt attcttgacc cgtatctccc caacagccat cttctgacca
     7861 attgcagttt gctcttttgc aactattttc atctgcatat tctttacatt gttttattaa
     7921 ataaaaagta gcgggatcgc ctaaaaaaac atagtcctca acactgatat tatttgcggt
     7981 ttcaattcgg cacattcttt tttgattaga aagtccgcaa ctattacaaa ggtctttatt
     8041 ggtggtgcta ttacaattaa aataaaagca gtatttttca attttataat ttccaaactc
     8101 ttctttttga acaggtattt gactttgttg cttaaaaaca aaatactctg ccaatgttaa
     8161 tcttttgcca aaaggagttt tcattaaatt ctcagctttt tttaaatcat tcaatccatt
     8221 ctctaaatca ttttttatgc cttctatccg acgctttgcc tcgtttaaac ttaaaaattt
     8281 ttcatttaca cctaaatttt cttttgctgt attataaact tctttttcat aattatcgtt
     8341 ggaataatct gtgggatctc cgttctcaat taatatcttg atttcttctc tttttatgtc
     8401 tatttctttt ctattactac agggatcata accgctttta aagatacctt cattggggtt
     8461 gcaagattta tccccacgag gacctccaca acaactgcaa tgagaatcac aataagagca
     8521 tccttcgctc ttttttgttg tgcaccatcc tgttgaacaa tttgaacact tgcatccgtt
     8581 ttttaataaa gaatttaatt catttgatag atctattaat ttttgagttt taatttcgat
     8641 agcatcaatt agattttgga gacattgata ctgatcattg ttttctaaaa gtttttcttt
     8701 atttccgttt tcatcatatt gatagcagta acggaaatct gtttcactgc taaaaatatt
     8761 ttcaacggcc ccctgaagca cctgcccctt ttgacaaata tattggccgg catgctgatt
     8821 atcgattata cattctgatt gctgacaatc aataagattt ctgtctttat caaaacagct
     8881 aatgtttttt gctaaaatgc tttcagcaat ggtgcccaaa ggaatctcct taaaaactaa
     8941 ttcaagtttt tgaggttcgg tgggggttag aggaccagaa ggtggtgttc caataatttc
     9001 aggcagttgg agagacacca aatcagggtt aatagtggtt aagatcaaat agatagcaaa
     9061 gagaattaaa agtccggaaa gggcactggt aagtttcttt ttggcactgc caatctgctc
     9121 aggattgccg gcagaggtaa gatataatac gcctccccag attaaaacac caaaagccac
     9181 taatccaccc agggccagag caatattaaa gatataattt acataactgg gcaaaccttg
     9241 ttctggggtt ggggcaggga cattgggcaa atcaggataa tctacctcat cagcaaaaa
//
LOCUS       DAOTQD010000019         9089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_6960_length_9089_cov_8.920412, whole genome shotgun sequence.
ACCESSION   DAOTQD010000019 DAOTQD010000000
VERSION     DAOTQD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6960_length_9089_cov_8.920412"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1501)
                     /locus_tag="P5570_01415"
     CDS             complement(329..1501)
                     /locus_tag="P5570_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR39400.1"
                     /translation="MKINLQKIFIFVLILVGGFWCGYFFNQKINIEAQQGANFNLLFK
                     TWNQIENKFYKFSEEKAEELKQEMLYGAIEGMVQSLNDPYSDFLRPEESLQLKEDLSG
                     SYEGIGAEIGIRDNILTVISPIKGTPAEKAGIMAGDKILEINGESTEEMTLTQAVMKI
                     RGKAGTEVNLKINRDEEILEIKVIRAKIDIPTLDFEMLNNNIAYIQLYNFYEDAPAEF
                     KTVSQEILKSGTNKIILDLRNNPGGYLSVATDIGNLFIERGKVILKEDRGRAGIDEIL
                     SSGPGAFSQFQVIILINEGSASASEILAGAIKENNPNTTTIIGKTSFGKGTVQEFIPL
                     SDGSSLKLTIAGWLLPSGESIDGKGISPDIEVEMTEDDVKAGRDPQLEKAIEEVSK"
     gene            complement(1577..2506)
                     /locus_tag="P5570_01420"
     CDS             complement(1577..2506)
                     /locus_tag="P5570_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRR39401.1"
                     /translation="MKKIIKIILIFIGIIIVLGGLIFFKGVNTFSAISSSDSLTSLKN
                     DDRWDILILGHQPTGGLTDSIMVLSYEKDSKKVVIFSIPRDLWVDIPDHGKEKINYAY
                     VAGQKNNSDGGGLKLAKEIVSNITGLDIDFAVAADVEALEEIVDTLGGIEVKEDRYFS
                     LDFYGNYVTIRPGINNLSGSETLAYVGSRATIGSDFGRMERQQKVLIAIKDKVTSLGL
                     LSRPDKIWNIFNSIEKHIETDIPLSQVKDLIEMASSMEIENIETIVFDTSNYLYSTHS
                     NGGAYILLPKAGNFSEIQTVCQNAFIEEEGEEK"
     gene            complement(2529..2602)
                     /locus_tag="P5570_01425"
     tRNA            complement(2529..2602)
                     /locus_tag="P5570_01425"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2566..2568),aa:Lys,seq:ttt)
     gene            complement(2705..4537)
                     /gene="glmS"
                     /locus_tag="P5570_01430"
     CDS             complement(2705..4537)
                     /gene="glmS"
                     /locus_tag="P5570_01430"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRR39402.1"
                     /translation="MCGIVGYIGEQKDPWIGIEALKRLEYRGYDSAGMAVFNPEKKEI
                     FSLKAAGKISSLEEKFSKSKISGSPFILHTRWATHGEPNEINAHPHPDCKKNIWVVHN
                     GIIENYQVLKQKLESLGHHFVSETDTEVLSHLIEHFFKGNLEEAVRKALKLVKGTYGI
                     LVIAKDDPEKIVAAKNSSPLVIAINNGDIFIASDPAAILTHSKKVIFLDDKEIAVLKP
                     GHFFVTDLNNHIKQKEFSEIEWSLEQTQKGGYPHFMLKEIMEQPDSLKDSQRGRIVLS
                     EGIAKLGGLENVKDKLREIERLIFVSCGTAYYAGLVGEYMIEEYAGIPVEVDLASEFR
                     YRKPILNKKTAVFAISQSGETADTLAAIREVKEKGVLALGIVNTVGSTIARETDAGVY
                     NHAGPEIGVASTKAFTSQLEVLALLTVLLGRQRDMSLVMGQRILKEILIIPELVKKVL
                     ILSPQIKELASKYKDVNDFLFIGRKYNHPIALEGALKLKEISYIHAEGYAAGEMKHGP
                     IALISEKFPTFAIAPSDSVYEKMISNIREIKARKGPVIALATEGNEEIKKIVDDVIYI
                     PKTLEMLTPLLSVIPLQLFAYYVGVSRGYDVDKPRNLAKSVTVE"
     gene            complement(4595..5395)
                     /locus_tag="P5570_01435"
     CDS             complement(4595..5395)
                     /locus_tag="P5570_01435"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HRR39403.1"
                     /translation="MKTYKKNDDSNNLIFLKLGGALITTPKPYTPHLLKIKEIAKEIY
                     QLRQKMNFQLLLGNGGGSFPHISAKKYKTKEGIVNKKSIRGISIVENDAAKLNRILIE
                     ELIRAGENAISIQPSSVFLTDNNTIKSYYLEPLKYYLKYDMIPVIYGDVVIDIKKGCT
                     ILSTEALFNFLAKKLKPKKIIMMSKVDGVYDSNKKIIPEINKDNFSKIKNFLKGADKI
                     DVTGGMLHKVEESVEIAKEGVEVCIIGGKKGNLKKCLKGEPIGTLIRY"
     gene            complement(5392..6291)
                     /gene="pyrF"
                     /locus_tag="P5570_01440"
     CDS             complement(5392..6291)
                     /gene="pyrF"
                     /locus_tag="P5570_01440"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRR39404.1"
                     /translation="MGKENFADKLLDKINVFKNPTCIGLDPRIEGVPYFLRKKEEKID
                     KILFNFNKMIIDATCDIVPVFKIQMAFYEKYKERGIKALENTIFYLRKKQKIIIIDGK
                     RNDIGPTADAYADAYLTKEGFDADAITVNPYLGSDGINPFIEKAIKYGKGVFVLVKTS
                     NPSSKDFQDKILKNGKRLYEEVAKMVNQWGRGTKGKNGYQVVGAVVGATYKKEAMILR
                     KIMPKSIFLVPGYGAQGGSAKDVMVNFDKNQKGAIINNSREIIFAWQSSPYKEKFGPQ
                     KFDMAAREATIKMKKDLSCAFQI"
     gene            complement(6303..7001)
                     /locus_tag="P5570_01445"
     CDS             complement(6303..7001)
                     /locus_tag="P5570_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39405.1"
                     /translation="MSNKKFNQQKFNQFIIENNVIGFFQQPITLKSGRKSHCYVNWRN
                     VSNDVFLMDRLTDFVIDFVKDKNFQPDCFYGVPEGATKLAILVQYKWAKKSPLYGPFS
                     HILAMGRGKPKDHGDEKDRFFIGVPKGKVIILEDVTTTGYSLLESINTIKKIKEASII
                     AAIGLTNRMEKGLDGKSVKESVEEKGVPYFQMSKLPELLFLACQKNNPSKEIAQKVEE
                     EFQQYGIEKLKLLN"
     gene            complement(6998..7924)
                     /locus_tag="P5570_01450"
     CDS             complement(6998..7924)
                     /locus_tag="P5570_01450"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRR39406.1"
                     /translation="MEKILFAGSSNFDKMAEKIAKASHIHFGNILIQKFACGEKYIQL
                     LDDVKGKIVYIYQTANENPDEIIMETLLMANAAKENGAKKIVLISPLLLYSRQDKKTT
                     KDKREPISAKLLARLYETAGIDKIITCHLHSDLILKFYKIPILNLKVYHLFAKELKKI
                     IKKQRDWQVVSPDEGARKDGQKFAQILGGLETAFFKKERESPDKKINMVSSLNFYGNI
                     KGKNVILFDDMVDTGGTIIKAKEKLILMGAKKIILAAVHPVLSGNAKEKLEKANFFKI
                     FFSNSVPINDCSNKLMKNLEIIDLTSEIKKHL"
     gene            complement(7934..>9089)
                     /locus_tag="P5570_01455"
     CDS             complement(7934..>9089)
                     /locus_tag="P5570_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRR39407.1"
                     /translation="YWGAPIPIIYCKDCGEVPVPEKDLPVKLPRVKDFKPSGDGKSPL
                     TNSKTFVNTICPKCGKKAKRETDTLDTFVCSSWYFLRYTDPKNKRKPFEKTKIKKWLP
                     VDLYIGGAEHAVMHLLYARFFTKALKDLGYLDFSEPFSKLFNQGTIYYKGAKMSKSRG
                     NVIRPDEIIKKYGSDTIRVYELFMGPADQSTEWSDRGVVGCYRFLQRVWNLQEKIQKP
                     KSKNQNQHLQKLLHKTIKKVSEDIENFRFNTAVSSLMILTNQLEKEKEIPKEYYEILI
                     KLLSPMAPHITEELWQRLHYKNKTVKRVLSIFDEKWPTYDLKIVEEEKITLVVQVNGK
                     KRDIVEVNKNISQKEIEKLIFQRENVKKYLQGKKVKKIIFVPKRLINILV"
BASE COUNT         2760 a         1646 c         1193 g         3490 t
ORIGIN      
        1 gtttaacgtg atacggtaaa cgttggcgtt gcgcgtaaac tattaaattc caaatcacaa
       61 attccaattt tcaatcaaat tccaagctca gcgaagccga gcgaagggcg aaaagaacga
      121 agttctttaa gctctaaatc ctaatgttca atggtgttgt acacttttgt ttagaatttt
      181 gaacattaga atttgattgg tcattggtta ttggagcttg gaaatttatg taataataat
      241 ttctgttttc tgatttctga caactgattt ctatttagtg tttagtattt gtttggaatt
      301 tggagtttgg agtttggagt ttggaaattt atttagatac ttcttctatt gctttttcaa
      361 gttgaggatc tcttcctgct tttacatcat cttctgtcat ttcaacttca atatctggtg
      421 aaattccttt gccgtcaatt gattcccctg atggcaataa ccaaccagca attgttaatt
      481 ttaaagaaga accgtcgctt aaagggataa attcttgaac tgttcccttg ccaaaacttg
      541 tttttccaat aatagtggta gtgttaggat tattttcctt tatggctcct gctaaaattt
      601 cagatgcaga agcagatcct tcgttgatca aaattattac ctgaaattga gaaaaagcac
      661 ctggtcctga tgataaaatt tcatctattc ctgctcttcc tctatcttct tttaatatca
      721 cttttcctct ttcaataaat aaatttccaa tatcagttgc caccgaaaga tatcctcctg
      781 gattatttct taaatcaaga ataattttat tagtgcctga ttttaatatt tcctgagata
      841 ctgttttaaa ttctgctgga gcatcttcat aaaaattata aagttgaata taggcaatat
      901 tattatttaa catttcaaag tccaaggtgg gaatatcaat ttttgccctg ataactttta
      961 tctctaaaat ttcttcgtct ctattgattt taagattgac ttctgtacct gcttttcctc
     1021 gaattttcat tacggcttgg gtaagagtca tctcttcggt gctctcgcca ttgatttcta
     1081 aaattttatc acccgccatt atacctgctt tttcagctgg tgtgccttta ataggtgaaa
     1141 taacagttaa tatattatct ctaattccaa tttcagcccc aataccctca taactgccgc
     1201 taagatcttc tttaagttgc aatgattctt caggtcttaa aaaatctgaa taaggatcgt
     1261 ttaagctttg gaccattcct tcaattgcac cataaagcat ttcttgcttt aattcttctg
     1321 ctttctcttc agaaaattta taaaatttat tttcaatctg attccaagtt ttaaaaagaa
     1381 gattaaaatt tgctccttgc tgggcttcaa tatttatttt ttgattaaaa aaatagccac
     1441 accaaaaccc accaacaagg attaaaacaa aaataaaaat tttttgcaga ttaattttca
     1501 tagattataa tttttaaagt taaagtaatt taaaacaacc aaacttttta cagctctatt
     1561 cttaattttt ttcttcttat ttctcctccc cctcctcctc aataaatgcg ttttggcaaa
     1621 ctgtttgaat ttctgaaaaa ttccctgctt ttggcaaaag gatataggcg ccaccattac
     1681 tatgtgttga atataaataa tttgatgtgt caaaaacaat tgtttcaatg ttttcaattt
     1741 ccatagagga agccatttct atcagatctt ttacctgtga caagggaata tctgtttcta
     1801 tatgcttttc aatgctgtta aaaatattcc aaattttatc aggtcttgaa agtaatccca
     1861 atgaagttac tttatccttt atagcaatta aaactttttg ttgccgttcc attcttccaa
     1921 aatcagatcc aatagtagct cgtgatccta cataagccag tgtttcactg ccagataaat
     1981 tatttattcc tggtcttatt gtcacataat ttccataaaa atctaaagaa aagtatcgat
     2041 cttcttttac ttcaattcct cctaatgtat ctacaatttc ttctaatgct tctacgtctg
     2101 cggcaacagc aaaatcaata tcaagacccg tgatatttga aacaatttcc tttgctaatt
     2161 ttaaacctcc gccatcagaa ttatttttct gaccagccac ataagcataa tttatttttt
     2221 ctttaccatg atctggaata tcaacccata gatccctagg aatagaaaaa ataactactt
     2281 ttttgctgtc tttttcatat gaaaggacca ttatactgtc tgtaagacca cccgtgggct
     2341 ggtgtccaag tattaatata tcccatcggt catcattttt aagagaagtt aatgagtcac
     2401 tactgctaat agcagaaaag gtatttactc ctttaaaaaa aattaatcct cccaaaacaa
     2461 tgattattcc tataaaaatt aaaattattt ttataatttt tttcataatt ttcattacct
     2521 tttatatgtg cgcccgccgc gactcgaacg cggaaccaat ggcttaaaag gccactgctc
     2581 taccaaattg agctacgggc gcaattaata tattttattt taatacaact tataatttta
     2641 atttaaaaat aaaaaaaatc aagccccttt ttaaaggcaa accaaaaaaa agaaaatagt
     2701 ccttttactc aacagttact gactttgcta aattacgagg tttatcaaca tcgtatccac
     2761 gagaaacccc tacataataa gcaaataatt gcaatggaat tacgctcaaa agaggggtaa
     2821 gcatttctaa tgtttttgga atataaataa catcatctac tatctttttg atttcttcat
     2881 ttccttcggt cgctagggca attacaggtc cttttcttgc ttttatctct cttatgtttg
     2941 aaatcatttt ttcataaacg ctatctgatg gcgcaatagc aaaggtggga aatttttcag
     3001 aaataagagc aattggtcca tgtttcatct caccagccgc ataaccctca gcgtgaatat
     3061 aagaaatttc tttcagcttc agtgcacctt ctagagcaat aggatggtta tattttcttc
     3121 caatgaataa aaaatcattt acatctttgt attttgaagc cagctctttt atttgcggac
     3181 ttaaaattaa aacctttttt actaattctg gaattattaa gatctctttc aaaattcttt
     3241 gacccattac taaactcata tctctttgtc tcccaagtaa aacggttaaa agtgctaaaa
     3301 cctcaagttg agaagtaaat gctttagtgg aagcaacacc tatttcagga cctgcatgat
     3361 tataaactcc agcatctgtt tccctggcta tggttgatcc aacggtatta acaattccta
     3421 aggccaaaac gcccttctcc tttacttctc tgattgctgc taaagtatca gctgtttctc
     3481 cgctttgaga aatagcaaaa actgctgttt ttttatttaa aataggtttt cgataacgaa
     3541 attctgaagc aagatcaacc tctacaggaa tcccagcgta ttcttcgatc atatattctc
     3601 ccactaaccc ggcatagtaa gcagttccgc aagaaacaaa aattaatctt tcaatctctc
     3661 gtaatttgtc tttaacattt tcaagtcctc ccaattttgc tattccctct gagagcacaa
     3721 ttcttcctct ttgagaatcc ttcaaactgt caggctgttc cataatttct ttaagcataa
     3781 agtgaggata accgcctttt tgtgtttgtt ctaacgacca ttctatttca gaaaattctt
     3841 tttgtttaat atgattgtta agatcagtaa caaaaaaatg tcctggtttt aaaacagcaa
     3901 tttctttatc gtcaagaaaa attacttttt tagaatgtgt taaaatagct gctgggtccg
     3961 aagcgataaa aatatcccca ttattaatag caataactaa gggactggaa tttttagcag
     4021 ccacaatttt ttctggatca tcttttgcaa taaccaaaat tccatatgtt ccttttacca
     4081 attttaatgc ctttcttact gcttcttcca ggttcccttt aaaaaaatgc tctatcaaat
     4141 gcgaaagcac ctcagtatca gtttcagaaa caaaatgatg acccaatgat tcaagttttt
     4201 gttttaaaac ttgatagttt tcaataatgc cattatgaac aacccagata ttttttttac
     4261 aatcaggatg aggatgtgca ttaatttcat taggttcgcc atgagttgcc cagcgagtat
     4321 gaagaataaa tggactgcca gaaatttttg attttgaaaa cttctcttct agtgaagata
     4381 tttttcctgc tgcttttaaa gagaatattt cctttttttc tggattaaaa acagccattc
     4441 ctgctgaatc atatcctcgg tattctaaac gttttaatgc ctcaatacca atccaaggat
     4501 ctttttgttc gcctatataa ccaacaatac cacacatatt tttattatta ataatctatt
     4561 ttaaaccttt taagggtata aagtcaagga ctttttaata tcttataaga gtgccaattg
     4621 gttcaccttt aagacatttt tttaaattac ctttttttcc acctattatg caaacctcaa
     4681 cgccttcttt tgcaatttca actgattcct ctactttgtg taacatacca cctgttacat
     4741 caattttatc agcccctttt aaaaaatttt ttattttaga aaaattatct ttgtttattt
     4801 ctggaattat ttttttattg ctatcgtaaa caccatcaac cttgctcatc attataattt
     4861 ttttaggttt taattttttt gccaagaaat taaaaagtgc ttctgtggat aatattgtgc
     4921 aacctttttt tatatcaatt actacatctc cataaattac tggaatcata tcatatttta
     4981 agtaatattt aaggggctcc aaataataac tttttattgt attgttatct gttaaaaata
     5041 ctgacgatgg ctgtatagaa atggcatttt caccagccct tattaactct tctatcaaaa
     5101 ttctatttaa tttagcagca tcattttcaa caatacttat tcctcgaata ctttttttat
     5161 ttactatccc ctcttttgtt ttatattttt tggctgatat atgaggaaac gatcccccgc
     5221 cattacccaa taataattga aaattcattt tctgccttaa ttgataaatt tcttttgcaa
     5281 tttcttttat ttttaaaaga tggggcgtat aaggtttagg ggttgtaatt aaagctccgc
     5341 ccaatttcaa aaaaatgagg ttatttgaat catcattttt tttatatgtt ttcatatttg
     5401 aaaagcacaa gaaagatctt ttttcatttt tattgttgcc tctcgagctg ccatatcaaa
     5461 tttttgtgga ccaaatttct ctttataagg actgctttgc catgcaaata taatttcacg
     5521 tgagttgtta ataattgctc ctttttggtt cttatcaaag ttgaccataa catctttggc
     5581 actgccaccc tgcgccccat atcctggtac taaaaaaata ctctttggca ttatttttct
     5641 taaaatcatt gcttcttttt tgtaggtggc tcctacaacc gcaccaacga cttgataacc
     5701 attttttccc tttgttcctc ttccccattg atttaccatt tttgctactt cttcatataa
     5761 ccgttttcca tttttaagta tcttgtcttg aaaatctttt gaagaaggat ttgaagtttt
     5821 aactaaaaca aaaacacctt ttccatattt aatagctttt tcaataaagg gatttattcc
     5881 atcactgcct aaatatggat taacagtaat tgcatcagca tcaaatcctt cttttgtaag
     5941 ataggcatca gcataggcat cagccgtagg accaatatca tttcttttac catctataat
     6001 aataattttt tgtttttttc ttaaataaaa aattgtgttt tccagggctt taatgcctct
     6061 ttctttatat ttttcgtaaa aagccatttg tatcttaaaa accggaacaa tatcacaagt
     6121 ggcatcaatg atcattttat taaaattaaa taatattttg tctatctttt cttctttttt
     6181 tcttaaaaaa taaggaacac cttcgattct tggatcaaga ccaatgcagg ttgggttttt
     6241 aaatacattt attttatcaa gtaatttatc agcaaaattt tcttttccca taattgatca
     6301 tcttaattaa gcaattttaa tttttcaatg ccgtattgtt gaaattcttc ttctactttt
     6361 tgagcaattt cttttgaggg attatttttt tgacaggcaa gaaataaaag ttcaggtagt
     6421 tttgacattt gaaaataagg aacacctttt tcttcaaccg actcttttac actttttcca
     6481 tcaagcccct tttccattcg attggttagt ccaattgcag caataatact tgcttctttt
     6541 atttttttaa tagtgttgat gctttcaagg agcgaatagc cagtggtagt tacatcttca
     6601 agtatgatta cttttccttt gggaacacca ataaaaaacc tgtctttttc atcgccatga
     6661 tccttaggtt ttcctctacc cattgcaaga atatgagaaa aaggaccata aagaggagat
     6721 ttttttgccc atttatactg aaccagaata gctaattttg ttgctccttc tggtacgcca
     6781 taaaaacaat ctggttgaaa atttttatct tttacaaaat caataacaaa atctgttaat
     6841 ctatccatta aaaaaacatc attgcttaca tttcgccaat taacataaca atgagatttt
     6901 cttcctgatt taagagtaat gggctgttga aaaaaaccaa tgacattatt ttctataata
     6961 aattgattaa atttttgttg gttgaatttc ttattactca taagtgcttt tttatttcag
     7021 atgtaagatc tataatctct agatttttca ttaatttatt actgcaatca tttataggaa
     7081 cagaattaga aaaaaatatt ttaaaaaaat ttgctttttc tagtttttcc ttagcgtttc
     7141 ctgacaacac tggatgtaca gcagccaata ttattttttt tgctcccatt aaaattaatt
     7201 tttcctttgc tttaataatt gtacctcctg tgtctaccat atcatcaaac aaaatcacat
     7261 ttttcccttt aatgttacca taaaaattaa gagaagaaac catatttatt tttttatcag
     7321 ggctctctct ttctttttta aaaaaagcgg tctcaagacc acctagtatt tgggcaaatt
     7381 tttgtccgtc ttttcttgcc ccttcgtctg gagatactac ttgccaatct ctttgttttt
     7441 taataatttt tttcaattct tttgcaaata aatgataaac cttaagattt aaaataggaa
     7501 ttttataaaa ttttaaaatt aaatcactat gaagatgaca ggtaataatc ttatctatcc
     7561 ctgctgtttc atataaacga gctaataatt tggcagaaat gggttctctt ttgtccttag
     7621 tagttttttt atcttgtctt gaatacaaaa gcagtggaga aatcaaaaca atctttttcg
     7681 caccgttttc ttttgcagcg tttgccatca aaagggtttc cattattatc tcgtcaggat
     7741 tttcgttcgc tgtttgataa atataaacta tttttccctt tacatcatcc aataattgaa
     7801 tatatttttc accacaagca aatttttgaa ttaaaatatt accaaaatga atatgagatg
     7861 ctttggctat tttttcagcc attttgtcaa aattagaaga gcctgcaaat aaaattttct
     7921 ccatagtaat attttatact aatatattta ttaatctttt gggcacaaaa attatctttt
     7981 taaccttttt cccttgaaga tattttttta cattttccct ttggaaaatt aatttttcaa
     8041 tttccttttg agaaatattt ttatttacct ccactatatc acgttttttc ccattaacct
     8101 gaacaaccaa agttattttt tcttcttcaa cgattttcaa atcatatgtt ggccattttt
     8161 catcaaatat tgataaaacc cttttaacag ttttgttttt ataatgtaat cgttgccata
     8221 actcttctgt aatatgagga gccattggag ataataattt tattaatatt tcataatatt
     8281 cttttggaat ctctttttct ttttctaatt gattggttaa aatcattagg ctagaaactg
     8341 ctgtattaaa cctgaaattt tcaatatctt cactcacctt ttttattgtt ttgtgcaaaa
     8401 gtttttgcaa atgttgattt tggtttttgg attttggttt ttggattttt tcttgtaagt
     8461 tccataccct ttgtaagaat cgataacacc ctactactcc cctatctgac cattctgttg
     8521 attgatcagc tggtcccata aatagttcat aaacccgtat tgtatcagat ccatattttt
     8581 tgataatttc atctggtcta ataacgtttc ctctggattt tgacattttt gcacccttat
     8641 aataaatagt accttgatta aaaagttttg aaaatggttc tgaaaaatca agatatccta
     8701 ggtcttttaa tgctttggta aaaaaacgtg cgtataaaag atgcattact gcatgttcag
     8761 ctccaccgat ataaagatcc acaggaagcc attttttaat tttggttttc tcaaatggtt
     8821 ttctcttatt tttgggatcg gtatatctta aaaaatacca agaagagcaa acaaaggtat
     8881 caagagtatc agtttctctc tttgcttttt taccacattt gggacaaatg gtattaacaa
     8941 aagtttttga atttgtcagt ggactttttc catctccgct tggtttaaaa tcttttactc
     9001 taggaagttt tactggcaaa tctttttctg gtactggcac ctcaccgcaa tctttacaat
     9061 aaataattgg tattggcgct ccccaatat
//
LOCUS       DAOTQD010000020         9058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_6987_length_9058_cov_8.041653, whole genome shotgun sequence.
ACCESSION   DAOTQD010000020 DAOTQD010000000
VERSION     DAOTQD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6987_length_9058_cov_8.041653"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1414
                     /locus_tag="P5570_01460"
     CDS             107..1414
                     /locus_tag="P5570_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRR39408.1"
                     /translation="MNYNKQFKLIFKNSFWLGVSEVVSKLVGFLIGIWMARRFGPDLY
                     GKWSFALAFVTIFSVVADFGLSTLSVRELARNKEKTKDYFLNITLLKFILGALSLGLM
                     ALIINLIEEDPETISLVYFLGIYIVINTFAAFFESIFRAREKMKYEAICRVSQSVLLF
                     SLAAFFIFNGFPILNISFAYISGSLLGTVLALVFVLRYFYPFKKNEASNGFAFSRFFD
                     LKFCKELLLEAWPFALSTVAIFLYNYFDQTMLGVMRGDKEVGLYSAAFKVVFTFQVFG
                     DVLAVAFFPQLSKKYKEGVKSLKEIFYHFQKIMLVFVVPFTILLFVFALPILRFLYGS
                     EYTAASNVFQVLVLSIAILLLSFPFGETLKAADRQKSYLWAIGSGAILNVVLNFIFIP
                     RFGMMGAAINTLISEIYIFIFLKIKLKKLWKELESRNSSSINL"
     gene            complement(1401..2366)
                     /gene="holA"
                     /locus_tag="P5570_01465"
     CDS             complement(1401..2366)
                     /gene="holA"
                     /locus_tag="P5570_01465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRR39409.1"
                     /translation="MILFLYGPDNFRSKKKLEEIINQHRLKYKSGLNFAKIESNKDEE
                     WFDQFKKRVETVSMFQENKLIILKNIFLSSKYLQDKFKDYLNEKKIFEKKDIILVVFE
                     EGEIKKTNTLFKFLLKNAFKKQEFKNLTPSQLKIWIQKEVKKHKGEIDDEAISSLITY
                     VGNDLWQIDQEIKKLIAFTNGKTIKKENVDALCKALIDPNIFETIESISKKDKKRALL
                     LISEHLEEGENEIKILSMIVYQFRNLLKLKDLATETKSFLNIYQLQRITGLHPFVIKK
                     TLPIAQKFSIEELKNIYQKLLKLDFQIKTGKIEPRLGIEMFITNL"
     gene            complement(2395..2688)
                     /gene="rpsT"
                     /locus_tag="P5570_01470"
     CDS             complement(2395..2688)
                     /gene="rpsT"
                     /locus_tag="P5570_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRR39410.1"
                     /translation="MPITKSAKKALRQNKKRKKENRAWKNRIKDSEKEIKKLVEEKKM
                     EEAKKALPGFYKIVDKAAKNNVIKKNTASRKKSKMAKAISKVESSNSKVKSSK"
     gene            complement(3012..3470)
                     /gene="greA"
                     /locus_tag="P5570_01475"
     CDS             complement(3012..3470)
                     /gene="greA"
                     /locus_tag="P5570_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRR39411.1"
                     /translation="MEKYLMSKKGLDKLKEELDYLKNVKRREIAQGLKKAASYGDLSE
                     NAEYQEMKELQSFVEGRILELEKTIDSAVVAPKSKDKDIVQIGSSVEVSCNGKKETFI
                     IVGPGEANPLEKKISIDSPLGSAFLNKKVGNIIEISTPRGKFQYKILKIE"
     gene            3695..4240
                     /locus_tag="P5570_01480"
     CDS             3695..4240
                     /locus_tag="P5570_01480"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39412.1"
                     /translation="MQYKICVSGAADTTHCNECALRLSKEVGKEIIRQGGVLVTGATT
                     GIPYWAAVGAKEEGGLSIGLSPAASEKEHIKKYRLPVDHFDVIVYTGFNYAGRNLLMT
                     RSSDGVIVICGRIGTLNEFTIAFEDDKPIGVLEGTGGTADMIRDIVKRGRRGSGKIVY
                     DRNPKRLVAKLMELIAKEKSL"
     gene            4255..4701
                     /locus_tag="P5570_01485"
     CDS             4255..4701
                     /locus_tag="P5570_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRR39413.1"
                     /translation="MDILNIPEVGFALKILLAFFLGALVGAEREHTGKEAGMRTFSLV
                     TISSTLFTILSREGFLGISNIFDPSRIAAGIVMGIGFLGAGVIIFKENHIEGLTTAAA
                     LWSAGGIGMAVGCGFYSLAAITTLVIIVILTLMKDVKIEKKADKNK"
     gene            4703..5827
                     /locus_tag="P5570_01490"
     CDS             4703..5827
                     /locus_tag="P5570_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HRR39414.1"
                     /translation="MDVKLLLSVATFCGTIIGVGVFALPYVVSQVGFWPGIFYLILLA
                     AAFVPLYLLYGETLLRTKGSARLPGLAERYLGKWGKIIISFSTFIGLSASLICYIIAG
                     GEFLSTIIGTDFFICALIFWFLLSLGIIFGLKSISQFELFVLFLFVITLGVLFFMCAP
                     HIRLENLSGFYPENLFLPYGVILFSMYGLSAIPALRDILKGEEKKLKNAILIGTILSL
                     VFYSVFVFIVVGVSGLETTEESIQGLIVKIGDGVILLGAIFGIMSVSTSFLTVGIYVR
                     DVLFYDFKARKWLSTAFICTVPLLGLALGIEYLVPLMSFIGSILGAFESVVLIFISKR
                     SKIKGDRKPEYQIKVPDPILYLICIVFVTGFIYEIVSFIK"
     gene            5905..6399
                     /locus_tag="P5570_01495"
     CDS             5905..6399
                     /locus_tag="P5570_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39415.1"
                     /translation="MKSPHNLPSHFPKPKKEESEFGKGKKDLLICKECNAVYFYKSWH
                     HRLEDYPYLKKTKNLKFTFCPACQMIKEKKFEGEVIVKNVSSKIKVNLINLIKNFGKE
                     AFKKDPQDRVISIIDNGSQLRILTTENQLAQRLGKKLSQVFKGRLSVSHSKGESTLRA
                     QVIL"
     gene            6668..8257
                     /locus_tag="P5570_01500"
     CDS             6668..8257
                     /locus_tag="P5570_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39416.1"
                     /translation="MKNNFTKKKKEIINETLIEKLNEGSLRKKVALIGIIEKIVQTGG
                     PTVFEINDGTGHLSLKGFVAPGKRAYPEIKEGDYIKAVVSLEEFNGEIEGDIREIFKK
                     TGEEELKLKNEIEKRKRNRARIPALEFLVNDPILIKLKNRFVSAATEIRMAIFDGRPI
                     IIRHHCDTDGYSAAFALERAILPLIEKQHMSEKAPWEFFMRAPSMSPYYEIDDSIRDT
                     AFSLRNVAKFSNKIPLIVIVDNGSSEGNLMAIKLGKIHGEKFIVVDHHHYDNDVISSE
                     VIEHINPFLVDESGAKISAGILCAELARFINPEVKNIEQIPALAALADRVDLENPTTV
                     ENYLEIAKKEGYSKNLLGDISLVIEYVSSKLRFMEAREYIEVLFGEPRDKQKVLIDLL
                     APYIKELDERGLKIGKANAKLEKINDIILQLVYIEETYPGFGFFPKPGRSIGLLHDNL
                     QSEKRLEKVISIGVMETAMTFRATNKSNFSIHELIKFLNEKFPEAFVEGGGHKNAGSI
                     KFLPNKKEKILALVKEFIKNS"
     gene            8263..8955
                     /locus_tag="P5570_01505"
     CDS             8263..8955
                     /locus_tag="P5570_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRR39417.1"
                     /translation="MTEVILKVKNLSVELGDEEIIKGLSFEVKKGNVLTILGPNGAGK
                     STLLKALLGILPYKGEIKWKDNIKIGYVPQRLPFIKDIPMSITEFFQLKNVGEKEIKE
                     ILGLVGFEENFLEKGIGDLSSGQFQRVLIAWALVGDPDVLLFDEPTSGIDISGKETIY
                     EFLERLKKEKKLTMLLVTHDLSVVYKFSNYVLCLNKCPICQGEPRQVLTSDSLQKLYG
                     QEVKFYEHHKHE"
BASE COUNT         2824 a         1225 c         1706 g         3303 t
ORIGIN      
        1 tttcatcatc attagtggac ataaaactat aatattattt tatttgtgtt tatactaata
       61 taaatatagt aaattatttt taaaataaag tcaatgatag tttaatatga attataacaa
      121 acaatttaaa ttaattttta aaaactcttt ttggctggga gttagcgagg tggtttccaa
      181 gttagtaggt tttttaattg gaatttggat ggcacggaga tttggtccgg atctttatgg
      241 aaaatggtct tttgctcttg cctttgtgac cattttttcg gtagttgctg attttggatt
      301 gtctactctt agtgttaggg aattggcacg caacaaggag aaaacaaaag attatttttt
      361 gaatattaca ttattaaaat ttatcttagg ggctttgtct ttgggtttaa tggctcttat
      421 tatcaattta atagaagaag acccagaaac aatttcattg gtttattttt tgggaattta
      481 tattgtaatt aatacttttg ccgcgttttt tgaatccatt tttagagcaa gggaaaaaat
      541 gaaatatgaa gctatttgcc gtgtttctca aagtgttttg cttttttctt tagcggcttt
      601 ttttattttt aacggatttc ccatattaaa tattagtttt gcttatatca gtggttcttt
      661 attgggaact gtattggctt tagtttttgt tctgcgatat ttttatcctt tcaaaaaaaa
      721 tgaggcaagc aatggttttg ctttttctcg tttttttgat ttaaaatttt gcaaagaact
      781 tcttttagaa gcgtggccat tcgctcttag cacagtagct atatttttat ataattattt
      841 tgatcagaca atgcttggtg taatgagagg ggacaaggaa gtaggtcttt acagcgctgc
      901 ttttaaagtt gtttttactt ttcaggtttt tggtgatgta ttggctgttg cttttttccc
      961 ccaactttca aaaaagtaca aagaaggtgt taagtccctt aaagagatat tttatcattt
     1021 tcaaaaaata atgcttgttt ttgttgtccc ttttacaatt ttgctttttg tttttgcttt
     1081 gcccattttg cgttttttgt atggatcaga gtatacggca gccagtaatg tatttcaagt
     1141 attagtttta tcaatagcaa ttttattgtt atcgtttcct tttggcgaga ccctcaaggc
     1201 agcagataga caaaaatcat atctttgggc tatcggcagt ggtgctattt taaatgttgt
     1261 tctgaatttt atttttattc caagatttgg aatgatggga gcagctatta ataccttaat
     1321 ttctgaaatt tatattttta tttttttgaa gataaaatta aagaaattat ggaaagaact
     1381 ggaaagtaga aattcatcat ctataaattt gtaataaaca tttcaatgcc caatcgcggt
     1441 tcaattttgc cggttttgat ctgaaagtca agttttaaaa gtttttgata gatgtttttt
     1501 aactcttcta tcgaaaattt ttgggcaatg ggaagagtct ttttaatgac aaagggatgc
     1561 aagccagtga ttctttgaag ttggtaaata tttaaaaatg attttgtttc tgttgctaaa
     1621 tcttttaatt tcaaaaggtt tctaaactgg taaacaatca ttgataaaat cttaatttcg
     1681 ttttctccct cttcaagatg ttctgaaatt aacaaaagag ctcttttttt gtcttttttg
     1741 gagatagatt caatagtttc aaaaatattg ggatcaatca atgctttaca caaggcatca
     1801 acattttctt ttttaattgt ttttccattg gtaaaggcaa taagtttttt tatttcttga
     1861 tctatttgcc acagatcgtt cccaacatag gtgattaatg acgaaattgc ttcatcatca
     1921 atttctcctt tgtgtttttt tacttctttt tgaatccata tttttaattg tgatggtgtg
     1981 agatttttaa attcctgttt tttaaaagca ttttttaaca agaatttaaa aagcgtattt
     2041 gtctttttta tttctccttc ttcaaacaca accaagataa tgtctttttt ttcaaaaatt
     2101 tttttctcgt taagataatc tttaaattta tcttgaagat atttggagga taaaaaaata
     2161 ttttttaaaa taatcaattt gttttcttga aacattgaaa cagtttctac tcttttttta
     2221 aattgatcaa accattcctc gtctttgttg ctttcaattt tggcaaaatt aagaccgctt
     2281 ttatatttta agcggtgctg gtttataatt tcttctaatt tcttttttga acgaaaatta
     2341 tcagggccgt aaagaaacaa tatcataatt agaatgtaga atgcgttttt tttgctactt
     2401 gctactttta actttcgaat tactactttc cactttcgaa atggccttgg ccatttttga
     2461 tttttttcga gaagcagtgt ttttctttat aacattgttt ttcgcggctt tatcaacgat
     2521 tttgtaaaaa ccaggaaggg ctttttttgc ttcttccatt tttttctctt ccactagttt
     2581 ttttatttct ttttcagaat cttttattct atttttccaa gcgcggtttt cttttttcct
     2641 ttttttattt tgtcgcaggg ctttttttgc tgattttgtg attggcatag tgatatttta
     2701 gcgttaaaac attaaactaa aatgattttt cttacgtcat tgcgcgtggc atcgcacgtg
     2761 gcacggctat tgtcatcgcg agcgagggag tgaaactttc aaagaagatt tcatcttctt
     2821 ttcaacgcga aacccgctgt ggcgggttcg cgtgaccgag cgtggcgatc ccgttgtatg
     2881 gttgcgggct gcgcccgaga ttgcttcgtc gctgcggctc ctcgcaaaga cagtggtggg
     2941 tgttgtcatc gcgaggattc cctcaccttt tatattatta taacaaaaat ttcattttta
     3001 agcaaaaaac tttattcaat ttttaagatt ttgtattgaa attttcctcg gggagtgctt
     3061 atttctataa tattgcctac ttttttattc aaaaaagcac ttcctaatgg agaatcaata
     3121 gaaatttttt tctctaacgg attggcttca cctggtccca caatgataaa tgtttctttt
     3181 ttcccattac aagatacttc aacagatgag ccaatttgga ctatgtcttt atctttgctt
     3241 tttggagcaa ccactgctga atcaattgtt ttttctaatt ccaaaattct gccctcaaca
     3301 aaggactgaa gttctttcat ctcttgatat tctgcatttt cactaaggtc gccataagaa
     3361 gcagcttttt ttaatccctg agcgatctct cttcttttaa cattcttaag ataatctaat
     3421 tcttctttta atttatctaa tccctttttg ctcattaaat atttttccat ataaatattg
     3481 taaacattat taccccctta tgatatcaaa tttttttaaa aatacaagag gttgcaaaga
     3541 aattttttta atgatagaat aataaaaatt ctgaataatt aatataaaaa acctaaattt
     3601 taaacaaact caaaagctga atcttataaa gtaagataaa gcaaaaaaat gaatacaaag
     3661 ttttttgtct tttgagtttt aattatttat tattatgcag tataaaattt gtgtttcagg
     3721 ggcagctgat actactcatt gcaatgaatg cgctctgagg ttatcaaaag aagtaggtaa
     3781 ggaaattatt cggcaaggtg gggtcctggt aactggtgct accacaggta ttccgtattg
     3841 ggcagctgtt ggcgcaaagg aagagggagg tctttcaatt ggtctttcac cggctgcttc
     3901 agaaaaagaa catattaaaa aataccgttt accagttgat cattttgatg tgattgtata
     3961 tactggattt aattatgccg gacgaaatct tttaatgaca agatcttctg atggggttat
     4021 tgtgatttgc ggaagaattg gcactcttaa tgaatttact attgcttttg aagatgacaa
     4081 acctattggt gtgttagaag gaacaggggg tactgctgat atgattagag acatagtaaa
     4141 gagaggtcgt cgagggtcag gaaaaattgt ttatgatcga aatccaaaaa ggttggtagc
     4201 aaaattaatg gagttaatag caaaagaaaa atcactgtaa tcatttttta aattatggat
     4261 attttaaata ttccagaagt aggttttgcc cttaaaattc ttttagcgtt ctttttgggg
     4321 gcgttggttg gtgcggagcg ggaacataca gggaaagaag caggtatgcg gacgttttct
     4381 ttagtaacaa taagttccac tctttttact attttatcac gagaaggttt tttaggaatt
     4441 agtaatattt ttgatccttc aagaatagca gctggtattg taatgggaat tggttttttg
     4501 ggtgcagggg ttattatttt taaagaaaat catatcgagg ggcttacaac agctgcagct
     4561 ctttggagcg cgggaggtat tggaatggct gtggggtgtg gcttttattc actggctgca
     4621 attactacat tagtaataat agtaatttta accttgatga aagatgttaa aatagaaaag
     4681 aaagcagaca agaataagta atatggatgt taaattattg ttgagtgttg ctacgttttg
     4741 tgggacaatt attggagtgg gtgtgtttgc tttgccctat gtggtttccc aggttggatt
     4801 ttggcctggt attttttatt taatcttgct agcagcagct tttgttcctt tatatctttt
     4861 atatggggaa actcttttac ggacaaaggg ttcggctcgg cttcctggat tagcagaaag
     4921 atacttggga aaatggggga aaatcattat ttctttttct acctttattg gtctttctgc
     4981 ttcgttgatt tgttatataa ttgcaggagg cgagtttttg tctacgatta ttggaactga
     5041 tttttttatt tgcgctttaa ttttttggtt tcttctctct ttgggtatta tttttgggtt
     5101 gaaaagcatt tctcagtttg aactttttgt gctctttttg tttgttatta cattgggggt
     5161 gctctttttt atgtgtgcac ctcatattag acttgaaaac ctttcagggt tttatccgga
     5221 aaatcttttt ttgccatacg gtgtaatact tttttcaatg tatggattat cagctattcc
     5281 tgctttgaga gatattttaa agggagaaga aaaaaaatta aaaaatgcta ttttgattgg
     5341 aacgatactt tcattggtat tttatagtgt ttttgttttt atagtggtag gagtatctgg
     5401 tttagaaacg actgaagaat caattcaggg attgattgtt aaaataggtg atggcgtgat
     5461 attattaggc gcgatttttg gaataatgag tgtttctact agttttctca ctgttggtat
     5521 ttatgtaaga gatgtgcttt tttatgattt taaagcaaga aaatggttat caactgcttt
     5581 tatttgcaca gtaccattgc tgggtcttgc tttgggaata gaatatttag tgccactaat
     5641 gagttttatt ggctcaatat taggtgcttt tgaaagcgtg gttttgattt ttatttcaaa
     5701 aaggtctaaa ataaaagggg acaggaaacc agaataccaa ataaaggttc cagatccaat
     5761 tttatattta atttgtattg tttttgtcac gggctttatc tacgaaattg tcagttttat
     5821 taaataatta aaataattaa aaaacaacat atcataactt ttccacgatc gtagaaaaat
     5881 tatgatatgt tgataaaagt gcatatgaaa tctcctcaca atttaccatc acatttcccc
     5941 aagcctaaaa aagaagaaag tgagtttggc aaggggaaaa aagatctttt aatttgcaaa
     6001 gagtgcaatg cggtttattt ttataaatcc tggcatcacc gacttgagga ttatccttat
     6061 ttaaaaaaaa ccaaaaatct taaatttact ttttgtccag cttgccaaat gataaaggaa
     6121 aaaaaatttg agggagaagt aattgttaaa aatgtatcct caaaaataaa agtaaatctt
     6181 ataaatttaa taaaaaactt tggcaaagaa gcgtttaaaa aagatcctca ggatcgggtg
     6241 atttctatta ttgataatgg ttcgcaatta agaattttaa ctactgaaaa tcaattagct
     6301 caacggttag gaaaaaaact tagtcaggta ttcaagggtc gtctttctgt ctctcattct
     6361 aaaggagaat ctactctcag ggcacaagtc attctttaaa ttatatactt tagtataggg
     6421 tgtctatcgc aaccctttga ttgcactcaa aggactatta atattattac ttcctgatcg
     6481 ataaagttta cctcctacag ttttccttca tcttgccatt ttttctcgct ttcagcgagc
     6541 ttatgtgagt aaagcgagtt gaagggttcc caaacgcctt agaaagtatt tttttaaaaa
     6601 ataacggaga atatttctcc gttattttgt cttttaatct tacctttaat gtggtagaat
     6661 atactatatg aaaaataatt ttacaaaaaa gaaaaaagaa ataataaatg aaactttaat
     6721 agaaaaacta aacgaaggat cactgcgcaa aaaggtggct ctgattggaa ttatagaaaa
     6781 aattgttcaa accggcggac caacagtatt tgaaattaat gacgggactg gccatctttc
     6841 attaaaaggt tttgtggctc ctgggaaaag ggcttatcca gaaataaaag agggagatta
     6901 tataaaagca gttgtttctt tggaagagtt taatggtgaa atagaaggag atattcgaga
     6961 aatatttaaa aaaaccggcg aagaagaatt gaaactaaaa aacgagattg aaaagagaaa
     7021 aagaaacagg gcacgtattc cggctttaga atttttggtt aatgatccta ttttaataaa
     7081 attaaaaaat cgatttgtca gcgcagctac tgaaattcga atggctattt ttgatggaag
     7141 accaataatt ataaggcatc attgcgatac agatggttat tcagcggcct ttgctctgga
     7201 aagggcaatt ctccctctaa tagagaaaca gcatatgtca gaaaaagcgc catgggaatt
     7261 ttttatgagg gctccatcaa tgtctcctta ttatgagatt gatgattcga ttcgagatac
     7321 agccttttca ttaaggaatg tggcaaagtt ttcaaataaa atacccttaa ttgttattgt
     7381 tgacaatggt tcctctgagg gcaatctaat ggcaataaag ctggggaaaa ttcatgggga
     7441 aaaatttatt gtggtagatc atcatcacta tgacaatgat gttatttcaa gtgaggtgat
     7501 tgagcatatc aatccatttt tagtagatga gagcggtgca aaaatttcag caggtatttt
     7561 atgcgctgaa cttgccaggt ttataaatcc tgaggtaaaa aatatagaac aaattcctgc
     7621 tttagcggct ttggctgata gggttgatct tgaaaatcca acaacagttg aaaattactt
     7681 agaaattgca aaaaaagagg gatattccaa aaatttatta ggagatattt ctctagtcat
     7741 agaatatgtt tcatcaaaat taaggtttat ggaggcgcgg gaatatatag aggttttgtt
     7801 tggtgagcca cgggacaagc aaaaagtgct gattgatttg ttagctccgt atataaaaga
     7861 attagatgaa aggggattga agataggaaa agcaaatgca aaattagaga aaatcaatga
     7921 tattattctg cagttggttt atatcgaaga gacctatccc ggatttggat tttttccaaa
     7981 gccgggacga agcattggat tattgcatga taatttgcaa agtgaaaaga gattagaaaa
     8041 agtgatttcg attggggtaa tggaaacagc aatgaccttt agagcaacta ataaatcaaa
     8101 tttttctatt cacgagttga ttaaattttt aaatgaaaaa tttcctgaag catttgttga
     8161 gggcggtgga cataaaaacg ctggttcaat aaagtttttg ccgaacaaaa aagaaaagat
     8221 tttggcattg gtaaaagaat ttattaaaaa ttcttaaaat ttatgacaga ggttattttg
     8281 aaggtgaaaa atttaagtgt tgaattggga gatgaagaaa ttattaaagg cctatccttt
     8341 gaagtaaaaa aaggaaatgt tttgacgatt ttaggaccaa atggcgctgg aaaaagtact
     8401 ttgttaaagg ctctgttggg aatattgcct tataaaggag aaataaaatg gaaagataat
     8461 attaaaattg gatatgtacc tcagcgtttg ccttttatta aagatattcc gatgtcaatt
     8521 acagagtttt tccaattaaa aaacgtggga gaaaaagaaa taaaagagat tttgggatta
     8581 gtagggttcg aagaaaattt tttagaaaaa ggaataggag atttatcatc aggtcagttt
     8641 caaagagtgt tgattgcctg ggcattagta ggagatcctg atgttttatt atttgatgaa
     8701 ccaaccagcg gtattgatat ttcaggaaaa gaaaccatat atgagttttt agaaagatta
     8761 aaaaaagaaa aaaaattaac aatgttgctg gttactcatg atctgagcgt tgtttataag
     8821 ttttcaaact atgtattgtg tttgaataaa tgtcctattt gtcaaggaga gccaaggcag
     8881 gttttaacaa gcgattcttt gcaaaaactt tatggccagg aagtaaaatt ttacgaacac
     8941 cacaagcacg aatgaaaagc aaaaatcaaa aagcaaaaag caaaatttat agttcggcac
     9001 gagattgata gatacttgct cgcttcgctc gcgatattca ttgtaacaat aaatatct
//
LOCUS       DAOTQD010000021         8682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_7345_length_8682_cov_9.142344, whole genome shotgun sequence.
ACCESSION   DAOTQD010000021 DAOTQD010000000
VERSION     DAOTQD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7345_length_8682_cov_9.142344"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1510)
                     /gene="murD"
                     /locus_tag="P5570_01510"
     CDS             complement(182..1510)
                     /gene="murD"
                     /locus_tag="P5570_01510"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRR39418.1"
                     /translation="MNMKLSFLEDKKILILGMGKEGKNTLSFLRKNFPEKVLGVADQK
                     DVKCQMSNVKIYFGKDYLKAIDDYDIIIKSPGISIHLPEIEKANKEKKLTSQTDIFFE
                     NCKGTIVGVTGTKGKSTTTTLIYQVLKEGGIRAHLIGNIGKPVLSYLFNSSKKDVFCY
                     ELSCHQLWNLKHSPHIAVFLNLFPEHLDYYKNFREYASSKANICRWQKADDFLIYNAN
                     DKNVKKIAQKSKAQKIPLKDYSNILKNVGIKKIPLLGEHNLLNIAAAIEVGKIFKIPN
                     LKIKRGIENFKPLPHRLELVGTFKGITFYNDALSTIPETAIAALDALGDKVETILLGG
                     YDRNINFDNLAKKILNSKIKNIILFPTTGEKIWRAVQKEFQKKNYQSRDRIKSFFVDN
                     MKDAVRIAFENTNQGKICLLSCASTSFSLFRDYKEKGNQFKKWVKYYGKT"
     gene            1641..2714
                     /gene="dprA"
                     /locus_tag="P5570_01515"
     CDS             1641..2714
                     /gene="dprA"
                     /locus_tag="P5570_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRR39419.1"
                     /translation="MEESQKKILNALNIAFSPSPESVIKILNHVSQPEKAFYLGEEKL
                     KKYGFKNDSVSNFLKKRKEIDIEKEWQRLKKENVRFITKDEKEYPTLLKEIAKPPIGF
                     YIKGHLLSNENYLASVGTRYPSDYGKMVVSEIIGEACQYFTIVSGMARGIDSFSHKAC
                     LLRGQRTVAVVGTGLDIVFPPENKNLAKEIENKGAIISEFPLGTPSLAYNFPLRNRII
                     AGMARGTVVIEGKINSGSLITANSALEESREVFAVPGPIFSKTSEGPNELIKQGAHPV
                     TKVSDILAVFNLKDAFNQEKEIKGETAEEDLILKILKEEMASTDEIIKKTKLDAGKVN
                     SALILMEIQGKIKRSGNTYTINN"
     gene            2747..3730
                     /locus_tag="P5570_01520"
     CDS             2747..3730
                     /locus_tag="P5570_01520"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF001985.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRR39420.1"
                     /translation="MPLFQLPRKLIKEFRQPFGTIVKGEDELAKEIKSQNYQTLVCIG
                     DFSNILLQKIGIMPNISIIDGKNERKKVNKKVIKKIKAPIVLKTKNPPSFVVNNAWNT
                     VKLSFCYTVPVTIFVSGEEDLLFLPACFFAPLNSLIVYGLKDKGGVVTKITPSLKKKI
                     KEYLEMGCKNFERVNEVIAGGTFDKLHLGHKFFLLSGAEKGKKLLVGITSKKYLAQYK
                     PINKNATPFAQRKSAVNNFLSERGFDFEIFKINNPIGIALQRGQAIVVSKDTQRGAEK
                     INQERKKKGLKPLKIIKIKTILAEDKKEISSQRIKNGEIDKNGRIIKNRAL"
     assembly_gap    3767..3866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4031..6100
                     /gene="topA"
                     /locus_tag="P5570_01525"
     CDS             4031..6100
                     /gene="topA"
                     /locus_tag="P5570_01525"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRR39421.1"
                     /translation="MLLIIVESPTKAHTIQKFLSGGDFIVRSSYGHVRDLPKSELGID
                     IENNFEPKYIIPQKAKKRVAELKKYLPKAEKIILATDEDREGEAIAWHLTQALGIEKS
                     KTPNPKAKTNSKPYERIAFHEITKEAIQKALNNPREININLVNAQQARRVLDRLVGYK
                     LSPFLWKKIQRGLSAGRVQSVAVKLICDREKEIEKFKPEEYWTIVASLLKSKKQKAKS
                     KNDQFKATLYKKDGKVIPKLGIKSEKEAKKIVEDLKNAEYKISHIEKKEIKRNPLPPF
                     TTSTLQQEAWQKLHFSARFTMQVAQSLYEKGLSTYHRTDSLNLSEQSLFQAKKFIENN
                     LGKNYWAGYFRRYKTKSKSAQEAHEAIRPTFPQKTVENLKSKLKPAEEKLYDLIWRRF
                     IASQMAEARFDSTIIEIQTSNSKSQIPNKSKIQNLSSNIQKPTYTFRANGQVLKFDGF
                     LKIYPLKFTEEELPPLEEKSPLKLIKLTPNQHFTKPPSRYSEATLIKELEKNGIGRPS
                     TYAPILSTIQERNYVEKDENKKLKPTEMGSLVNNLLCEHFPQIVDIQFTANMEKDLDK
                     IAKGDIRWQEPIKKFYFPFEENLKAKEKEIEKIDLTEKTNELCPECKNPIVIKMGRYG
                     KFYACSNFPKCKFTKKYLEKIDLACPKCKKGEVIIKKTKKGKTFYGCSRYPKCDFASW
                     QKPKNKI"
     gene            6168..7082
                     /locus_tag="P5570_01530"
     CDS             6168..7082
                     /locus_tag="P5570_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRR39422.1"
                     /translation="MLKVVQSEKVIWIDIMKPTEEDVKYLEDNFFFHPFILKSIIPPI
                     RHPRFENYGDYLFLVLHYPFFEKRCRETKPRELDILVTKNTLITIHYHTILPLRAFFT
                     RLSLYDNEKEEFTDEGVGEVLYRFLNEILKASFPKLDRIDEKIDNIEKEVFQSKQKEV
                     IKEISILKHDLIDFQRIIQPQSAVFEGLKQTSGKFFGEHFYPYFNELFNCFLTIKEVL
                     QTHHQTLNELEETNSNFLSTRTNEIIKILTIFTVVLTPLALIANVFGMNTTYLPFTEN
                     ISGFWIVIGLMIFSAILMIAYIKKKKWI"
     gene            7108..7803
                     /locus_tag="P5570_01535"
     CDS             7108..7803
                     /locus_tag="P5570_01535"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRR39423.1"
                     /translation="MNIGIALLDFLFPRFCLGCNKEKTYLCPDCFSKIEIFPAPFCPY
                     CNKRTYNGEICSNCKKTLSGFAAAVPYSNKLIEKLIDAFKYRFVKELANPLAFLIWKF
                     LKENQEISFVKNPLDFIIIPIPLHKRRQRERGFNQAEELGKVLSPLLKITMKNDVLFR
                     QRYTKPQARLKTGREENIKNAFIVNTNESIIGKKIILIDDVATSLSTLEEAAKVLKNG
                     GAKEIWGLTVAKG"
     gene            complement(7804..>8682)
                     /locus_tag="P5570_01540"
     CDS             complement(7804..>8682)
                     /locus_tag="P5570_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRR39424.1"
                     /translation="IGLIIGETASLPKEIIKKFQMIFVPFATDWPEGKDLPGKDLFQK
                     MREGAKKGIKNFPKTSQPSIGNYKEAFEKALSKFEKTILITVSSKLSGAYNTACFARR
                     MLSQKMQERIEIIDSQTGSAGEGLLVFKTAQLIEKGKKEFNEIVLSIKQMIPEIHLFC
                     MLEDPKWLEVGGRLSHPLAVLLRQMQKIGMRPLIGTKNGMIKPVSLKIKAKDVPTALF
                     KELKNETRKKLESGKKIIVAISHADNLKGAKRLQKMIEKRLNNTKIAFLSLVDPVIGV
                     HSGPGTLLCAWTEEDL"
BASE COUNT         3099 a         1436 c         1344 g         2703 t
ORIGIN      
        1 gattgcttcg tcgctcacgc gagcccgctg aaagcgggcg aagcgttgaa aagaagatga
       61 aatcttcttt gaacgctcct cgcaatgaca gtgaaaaaga agttttcttc ttttcaacac
      121 ttcgctgcgc tccgctcgtg tcaatgacgg tttactaatt ttgaaaattg aaaatttcta
      181 tttaagtttt cccatagtat tttacccatt ttttgaattg attgcctttt tctttgtaat
      241 cgcgaaacaa actaaaactg gttgaggcgc aagacaaaag acaaatcttt ccctgattgg
      301 tgttttcaaa agcgatcctt acagcgtctt tcatattatc gacaaaaaat gacttaatac
      361 gatctcttga ttgataattt tttttctgaa attctttttg aacagctctc caaatttttt
      421 ctccagtggt tggaaataaa ataatatttt tgatttttga atttaaaatt ttttttgcta
      481 gattatcaaa attgatattt ctgtcgtaac cgcccagtaa aatggtttct actttatccc
      541 ccaaagcatc caaggcagcg atagcggttt caggaattgt tgataaagca tcattataaa
      601 aggtgattcc tttaaaggta ccaaccaatt ctagtcgatg cggcaagggt ttgaaatttt
      661 caatacctct tttaattttt aaattaggaa tcttaaaaat ttttcctacc tcaatcgctg
      721 cagcgatatt taaaagattg tgttcgccaa gaagaggtat ttttttaatt ccaacattct
      781 tgagaatgtt ggaataatct ttaagaggaa ttttctgagc ttttgatttt tgagcgatct
      841 tttttacatt tttatcattg gcattgtaaa tcaaaaaatc atctgctttt tgccaacgac
      901 aaatatttgc tttgcttgaa gcgtattctc gaaaattttt ataatagtcc aagtgttcag
      961 gaaacagatt caaaaaaact gcgatatggg gtgaatgttt taaattccat aattgatgac
     1021 aagataactc atagcaaaaa acatcttttt ttgaggaatt aaaaagatag gataaaaccg
     1081 gctttccaat attgccgatc aaatgcgccc taataccgcc ttcttttagt acttgataga
     1141 tcaaagtagt agtagtactt tttccctttg tgccagttac accaacaatg gtacccttac
     1201 agttttcaaa aaaaatatcg gtttgggagg ttaatttctt ttctttatta gctttttcaa
     1261 tttctggaag atgaatagaa atccctggtg attttattat aatatcgtaa tcatcaattg
     1321 cctttaaata atccttgcca aaatatattt tgacatttga catttgacat ttgacgtctt
     1381 tttgatcagc tactcccaaa accttttctg gaaaattttt tctcaaaaaa gatagggtat
     1441 ttttgccctc tttccccatt cccaaaatca aaattttttt atcctctaaa aaagaaagtt
     1501 tcatattcat aattatagca attttaaaag catataccaa aaagaaacaa ttgcaaatat
     1561 ataaatttat aatatattaa ttttgtatat tggtgatcac tccttgctat tttgaagaaa
     1621 aaaaattatt attaaaaaat atggaagaat ctcagaaaaa aatcttaaac gccttaaaca
     1681 ttgccttttc tcctagtcca gaaagcgtta taaaaattct caatcatgtt tctcaaccag
     1741 aaaaagcatt ttaccttggt gaggaaaaat taaaaaaata tggatttaaa aatgatagtg
     1801 tctctaattt tttaaaaaaa agaaaggaaa ttgatataga aaaagagtgg caacgattaa
     1861 aaaaagaaaa tgtaagattt attacaaaag acgaaaaaga atatcctacc ctcttaaaag
     1921 aaattgccaa gccccctata ggtttttata tcaaaggaca tttgctttcc aatgaaaatt
     1981 atcttgcttc ggttggtacc cgatatccgt ctgattatgg aaaaatggtt gtttctgaaa
     2041 ttattggaga agcttgtcaa tattttacaa tagtttctgg aatggcaagg ggcattgata
     2101 gcttctctca caaggcctgc cttttaagag gacaaagaac agttgcagta gttggaacag
     2161 gacttgatat tgtttttccg ccagaaaata aaaatttagc aaaggaaatt gaaaacaaag
     2221 gagcaattat ttctgaattt cccctgggca caccgtcttt agcatataat tttcctttaa
     2281 gaaatagaat tattgctgga atggcaaggg gcacagtagt gattgaagga aaaataaata
     2341 gcggttcctt gattactgcc aattcagcgt tggaagaaag tagagaagtt tttgctgtac
     2401 ctggtccaat attttctaaa acttcggaag gaccaaatga gttgattaaa caaggtgctc
     2461 atcccgtaac aaaagtcagt gatattttgg ctgtttttaa tttaaaagac gcttttaatc
     2521 aagaaaaaga aataaaagga gaaacagcag aagaagatct tattttgaaa atactcaaag
     2581 aggaaatggc ttccactgat gaaattatta aaaaaacaaa actagacgca ggcaaagtaa
     2641 attctgcttt aatcttaatg gaaatacagg gaaaaataaa aagaagcgga aatacttata
     2701 ctataaataa ttaatctaat gaagttaaaa gtagttctaa tgttttatgc ctctgtttca
     2761 actgccccga aaattaatta aagaatttcg acagcctttt ggcacaatag taaaaggaga
     2821 agatgaacta gcaaaagaaa taaaaagcca aaattatcaa acccttgttt gcattggcga
     2881 ttttagtaat atcttgttac aaaaaatagg cattatgcct aatatttcga tcattgatgg
     2941 gaaaaacgaa agaaaaaagg tgaataaaaa agtaataaaa aaaatcaaag cacctattgt
     3001 tttaaaaaca aaaaatcctc cttcttttgt tgttaataat gcatggaata cagtaaaact
     3061 atctttttgt tatactgtac ctgttacaat ttttgtaagc ggagaagaag atcttttgtt
     3121 tttgccagct tgcttttttg cgccgttaaa ctcacttatt gtatatggat taaaagataa
     3181 agggggcgta gtaacaaaaa tcacaccatc acttaaaaag aaaattaaag aatatttaga
     3241 gatgggctgt aaaaattttg aaagagtaaa tgaagtaatt gctggtggca catttgataa
     3301 attgcacctg gggcacaaat tttttttgct cagtggcgct gaaaaaggta aaaaattatt
     3361 agtaggtatt acttcaaaaa aatacctggc tcaatacaag ccaataaata aaaatgctac
     3421 gccctttgct caaagaaaaa gtgctgtaaa taatttttta tcagaaaggg gctttgattt
     3481 tgaaatattt aaaatcaata atcctattgg aattgctctc caacgtggac aagccattgt
     3541 ggtctcaaaa gatactcaaa gaggcgcaga aaaaattaat caggaaagaa agaaaaaggg
     3601 gttaaaaccg ctcaagatta taaaaataaa aactatttta gcagaagata aaaaagaaat
     3661 ttcttcccag agaataaaaa atggagaaat agataaaaac gggagaataa tcaaaaatcg
     3721 agccttgtag taacaaggtg agacgttcaa agaatttcat tcttttnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnngttc aaagaatttc attcttttcg cgtttcgact
     3901 gttcaaagaa tttcattctt tttacgtctc agttgaaact ccaacggaag aagagaacaa
     3961 gattctcttt acccgtttcg ttttgcttca ttcaactcaa cttttacatt ttgattttta
     4021 ctttattttt atgcttctta ttattgttga aagccctacc aaggcacata ccattcaaaa
     4081 attcctttcc ggaggtgatt ttattgtgcg ctcatcatac ggccatgttc gtgatttgcc
     4141 aaaaagcgaa ttgggcattg atattgaaaa taattttgaa ccaaaatata ttattcctca
     4201 aaaagcaaaa aaaagagttg ctgaattgaa aaaatatttg ccaaaagcag aaaaaataat
     4261 tttggctact gatgaagata gagaaggaga agcaatcgcc tggcatctga cccaagccct
     4321 gggtattgaa aaatccaaaa ctccaaaccc caaagccaaa acaaactcaa aaccttatga
     4381 acgaatcgcc ttccatgaaa ttaccaaaga agcaatacaa aaagccctta ataatcctcg
     4441 ggagattaat ataaatttgg ttaatgccca gcaagcaaga agagttttag accggctggt
     4501 tggttataaa ctttctccgt ttctatggaa aaaaatacaa agagggcttt cagctggccg
     4561 ggtccaatca gtagctgtaa aattgatttg cgacagagaa aaagaaatag aaaaatttaa
     4621 accagaagaa tactggacaa tcgtcgcttc gctcctcaaa agcaaaaagc aaaaagcaaa
     4681 aagcaaaaat gatcagttta aagcaacgct ttataaaaaa gacggtaagg ttatcccaaa
     4741 attaggaatt aaaagtgaaa aagaagcaaa aaaaattgtt gaagatttaa aaaacgcaga
     4801 atataaaatc agccatattg aaaaaaaaga aataaagcgc aatccccttc caccatttac
     4861 tacaagcacc ctgcaacaag aggcctggca aaaacttcat ttctcggcaa gattcacaat
     4921 gcaagttgct caatcgttat acgaaaaagg cttatctaca tatcatcgta ctgattctct
     4981 aaatttatct gaacaaagtt tattccaggc aaaaaaattt attgaaaata atttaggtaa
     5041 aaattactgg gctggttatt ttcgacgcta caaaacaaaa tcaaaatctg cccaagaagc
     5101 ccacgaagca ataagaccca ctttccctca aaaaactgtt gaaaatttaa aatcgaaatt
     5161 gaaaccagca gaagaaaaat tatatgattt aatctggaga agatttattg catcgcaaat
     5221 ggcagaagcc cgtttcgatt caaccattat tgaaatccaa acctcaaatt ccaaatcgca
     5281 aattccaaac aaatctaaaa tccaaaacct atcatctaat atccaaaaac caacttatac
     5341 ttttcgcgca aacggccagg ttttaaaatt tgacggattt ttaaaaattt atccattaaa
     5401 atttactgag gaggaattac ctcccctaga agagaaaagt ccgttaaaat taataaaact
     5461 tactcccaat caacacttta caaaaccgcc ttcacgctat agcgaagcca ccctgataaa
     5521 agaattggaa aaaaatggca ttggccgacc atcaacttat gcgccgattt tatcaacaat
     5581 tcaagaaaga aattatgtag aaaaagatga aaataaaaaa ttaaagccaa cagaaatggg
     5641 aagtttagtg aacaatcttt tgtgcgagca ttttcctcaa atagtagata ttcaatttac
     5701 tgctaatatg gaaaaagact tagacaagat cgccaagggt gatatccggt ggcaagaacc
     5761 tattaaaaaa ttttattttc cttttgaaga gaatcttaaa gccaaagaaa aagagattga
     5821 gaaaatcgat ctcacagaaa aaacaaatga actttgccct gaatgcaaaa accccattgt
     5881 tattaaaatg gggcgctatg gaaaatttta tgcttgctct aattttccaa agtgcaagtt
     5941 tacaaaaaaa tatcttgaaa agattgatct tgcctgtccc aagtgcaaga aaggagaggt
     6001 cattattaaa aaaactaaaa aaggaaaaac tttttatggt tgctctcgat atccaaaatg
     6061 cgactttgca agttggcaaa aaccaaaaaa taagatataa tattacctat attataatat
     6121 catttacaaa ttacgaattt catacaaatt tatacaaata aacttttatg cttaaagtcg
     6181 ttcagtcaga aaaagttatt tggattgata taatgaaacc aacagaagaa gatgtaaaat
     6241 atttagagga taatttcttc tttcatcctt ttattttgaa aagtattatt cctcccatac
     6301 gccatcccag atttgaaaat tatggtgatt atcttttctt agtattgcac tatccatttt
     6361 tcgaaaaaag atgccgtgaa accaaaccgc gagagcttga catcttagtg actaaaaaca
     6421 ccttaattac tattcattat catacaatat tacctctcag ggcattcttt acccgacttt
     6481 ctctttatga taacgagaaa gaagagttta ctgatgaagg cgtaggagaa gtgctttatc
     6541 gatttttaaa tgaaatctta aaagcatctt ttcccaaatt agaccgcatt gatgaaaaaa
     6601 tagataatat tgaaaaagag gtctttcaaa gcaaacaaaa agaggttatt aaagagatct
     6661 caatattaaa acacgattta attgattttc aaagaattat ccagccccag tcagctgtgt
     6721 ttgaaggatt aaaacaaaca tcaggaaaat tttttggtga acatttttat ccttacttta
     6781 atgaactttt taattgtttt ttaaccataa aagaagttct tcaaacccat catcaaaccc
     6841 ttaatgagct agaagaaaca aactctaatt ttctttctac tcgcactaat gaaattatta
     6901 aaattttaac catctttaca gttgttttaa cacccttggc ccttatagct aatgtctttg
     6961 gaatgaatac tacctatctt ccatttaccg aaaatatttc tggtttttgg attgttatag
     7021 gactaatgat tttttccgcc attttaatga ttgcatatat taaaaagaaa aaatggatat
     7081 agcagttttg ttctcggacc tttatttatg aatatcggca ttgctttatt agactttctt
     7141 ttcccaagat tttgtttggg ctgtaataaa gaaaaaacct atctttgtcc ggattgtttt
     7201 tcaaaaattg aaatctttcc tgctcccttt tgcccttatt gcaataaaag aacctacaat
     7261 ggagaaattt gtagtaattg caaaaaaacg ctttctggtt ttgctgctgc agtgccctat
     7321 agcaacaaac ttattgaaaa attaatagac gcctttaaat atcgatttgt aaaagaatta
     7381 gccaatccat tggctttttt aatttggaaa tttttaaaag aaaatcaaga aatttccttt
     7441 gttaaaaatc ctttggattt tattattatt cctattcctt tgcacaaacg acgccaaaga
     7501 gaaagaggat ttaatcaagc agaggaatta ggaaaagtgc tctcaccgct tttaaaaatt
     7561 actatgaaaa atgatgttct tttccgtcaa agatacacca aacctcaggc ccgtttgaaa
     7621 actggcagag aagaaaatat aaaaaacgcc ttcattgtca atactaatga aagcattata
     7681 ggaaagaaaa ttattcttat tgatgatgtt gcaacatcac tttccacctt agaagaagcg
     7741 gcaaaagtac ttaaaaatgg cggggcgaaa gaaatttggg gattaacggt agctaaaggg
     7801 taattaaagg tcttcttctg tccaggcgca aagcaaagtt cctggcccgc tgtgaacacc
     7861 tatcactgga tcaaccaaac ttaaaaaagc gatctttgta ttatttaatc ttttttcgat
     7921 cattttttgc aatctttttg ctccttttaa attatctgca tgagagattg ctacgataat
     7981 ttttttaccg ctttctaatt tttttcttgt ttcatttttt aactccttaa acagcgctgt
     8041 tggcacatcc tttgctttaa tctttaaaga taccggtttt atcataccat tttttgttcc
     8101 aatcaatggt ctcattccaa ttttctgcat ttgtcttagt aaaactgcta agggatgaga
     8161 caatcgtcca ccaacctcca accactttgg atcttccagc atgcaaaaca aatgaatttc
     8221 aggaatcatc tgttttattg ataaaacaat ttcattaaat tctttttttc ctttttcaat
     8281 caactgggca gttttaaaaa ctaacaatcc ttcgcccgct gaaccggttt gagaatcaat
     8341 aatttcaatt ctctcttgca ttttttgcga aagcatcctt cttgcaaaac aagccgtgtt
     8401 gtaagcccct gataattttg aagaaactgt aatcaaaatt gttttttcaa attttgaaag
     8461 cgctttttca aatgcttctt tataattacc gattgagggc tgagaggttt taggaaaatt
     8521 tttaattccc ttctttgctc cttctctcat tttttgaaac aggtccttgc caggtaaatc
     8581 ttttccttct ggccaatcag ttgcaaaagg aacaaaaatc atttgaaact tttttataat
     8641 ctcttttggc agagaagcag tttcaccaat aattaaacca at
//
LOCUS       DAOTQD010000022         8655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_7375_length_8655_cov_7.334070, whole genome shotgun sequence.
ACCESSION   DAOTQD010000022 DAOTQD010000000
VERSION     DAOTQD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7375_length_8655_cov_7.334070"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1109)
                     /locus_tag="P5570_01545"
     CDS             complement(66..1109)
                     /locus_tag="P5570_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRR39425.1"
                     /translation="METKVAVIGVGGRTGTMFAYELQNAAEVVGVARKEEIDLIKKKK
                     LYIEKSGSQRELFQGRVISEDEFPGKELPDIIFLATKNPVGLPVKSYYQKISDFLKGK
                     EEKLPSLVLSQNGIAAGKEALTALKEVFGEKGESIQIIRVCLLNPIEKKIIKNDLEAS
                     SKKDFGNVCINYFLPIRLTFGVFSGSKDTQKIEKVFKEAHIEAQKVLPEDVTNMEFSK
                     LFSNLLGVVSAVEGLSIKDGFKKKDVFVEEINVLREYIKVVKTSGGRFLNLPNAPIAF
                     LATLIEKTPLPVLGAVRSKIGSMISKGRGGKPKGNLDEIDYYIGAIVELGKTLNIDTP
                     SSKKVLKKTLKKL"
     assembly_gap    1147..1246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1253..2275)
                     /locus_tag="P5570_01550"
     CDS             complement(1253..2275)
                     /locus_tag="P5570_01550"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRR39426.1"
                     /translation="MAKKFDQSNMRQVILDFPKQFKVGVEAAKNIKTSANRSLFSNII
                     ICGMGGSALPGDVFVMINNHLLLTNLPVFIHQNYGLHPMTNKNSFVMCISYSGNTEET
                     ISAFKEARKKKLSIAAIASGGKLIELAKKNNVPFAQIAAGNQPRCALGYQFSALIKIL
                     SNFKLIKNLDKELFALEKNLAPHKLEKTGLSLAKKLKGKIPLVYSSEDNKCLARIWKI
                     KFNENSKIPSFYNSLPELNHNEMTGMGDCEIKEWRELFQVIFLSDKTDHPRIIKRMNI
                     LSEIFQKRNIKTNFVQIEGKNILDKIFNNNLIADWTSYYLAVSRNIDPTPIKLVEEFK
                     KKMADE"
     gene            complement(2295..2367)
                     /locus_tag="P5570_01555"
     tRNA            complement(2295..2367)
                     /locus_tag="P5570_01555"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2331..2333),aa:His,seq:gtg)
     gene            complement(2373..2446)
                     /locus_tag="P5570_01560"
     tRNA            complement(2373..2446)
                     /locus_tag="P5570_01560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2410..2412),aa:Arg,seq:tct)
     gene            complement(2634..3308)
                     /locus_tag="P5570_01565"
     CDS             complement(2634..3308)
                     /locus_tag="P5570_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRR39427.1"
                     /translation="MKNSNPLVIMMLGLPGSGKGTQANLFVERFKLDYIGSGDLLRER
                     KKEKDFTGKKIRQTIDVGGLVPTPVIFKLWLDQFEKLKSTKKNFNGLVIDGSPRKILE
                     AYLIDESLNWYEWDKNVKIFLIKISIKESFNRLTKRRQCKKCGRVIPWAGDFKKLKTC
                     DQCGGKLFTRPDDNIESIKKRIEKYKKETMKVIKYYKKQGKLIEINGEQGVEEVFQEI
                     AEKIKS"
     gene            complement(3348..4622)
                     /gene="secY"
                     /locus_tag="P5570_01570"
     CDS             complement(3348..4622)
                     /gene="secY"
                     /locus_tag="P5570_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRR39428.1"
                     /translation="MDFSKIIAIFKYRDLRSKVLFVLAMMVVFRMAANIPIPGVDRDA
                     LASFFETNQFFGLMNVFTGGTLENLSIVMLGLGPYITATIIMQLLTLIFPKLKEMYQE
                     SGEEGKRKFTQYGRILTVPLAALQSFAMLRLLQSQGALGQFTTSSLVNSIITITAGCV
                     FLMWIGELITEKGIGNGISLLIFAGIISSVPAQIGQTIMTWDPSHLPSYLLFLAASII
                     IIVGVVIINEARRNVPVSYAKRIRGRRMYGGAQTYLPMMINPAGVIPIIFALSIMMIP
                     GMFGSMFADTSIPFVSGFANIINNLFQNQLIYGVCYFILVVLFTYFYTAVTFDPESIA
                     DNLQKMGGFIPGLRPGKQTAQFLSHILNRVLLIGALFLGLIAVMPSIIQGFTGITTFN
                     FAVGGTSLLIIVSVILETSKQIKSQLEMREYD"
     gene            complement(4836..4955)
                     /locus_tag="P5570_01575"
                     /pseudo
     CDS             complement(4836..4955)
                     /locus_tag="P5570_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(5029..5478)
                     /locus_tag="P5570_01580"
     CDS             complement(5029..5478)
                     /locus_tag="P5570_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRR39429.1"
                     /translation="MQNKDEFESQLLDLARVTRVTAGGRRLRFRATVVVGNRAGKVGI
                     GVAKGKDVSEAVEKATTKAKKSLISVPLKNETIPYDIEVKYQSARVFLRPQRKGSGLV
                     VGGAMRTVCELAGVPNITGKLISKTNNKINIARATLKAFQEIEMRSK"
     gene            complement(5593..5949)
                     /gene="rplR"
                     /locus_tag="P5570_01585"
     CDS             complement(5593..5949)
                     /gene="rplR"
                     /locus_tag="P5570_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRR39430.1"
                     /translation="MKNFQKDIKKKRDRRHKRVRAKIKGTNMRPRLSVFRSNTHISCQ
                     LINDNEGKTIISADDKEIKEKGKKTDIAFEVGKLIAKKGKEKGIEKVVFDRGYYRYHG
                     RVKALAEGARKGGLQF"
     gene            complement(5979..6527)
                     /gene="rplF"
                     /locus_tag="P5570_01590"
     CDS             complement(5979..6527)
                     /gene="rplF"
                     /locus_tag="P5570_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRR39431.1"
                     /translation="MSKIGKQSIQILEGTKVEIKGNEIFIEGPKGKMKYVLPPEIEVE
                     KKGNEILVRFKGSRDKSKKALWGTWQRHIKNAVIGVNKEFEKELKVEGVGWRVELKGN
                     KLVLKIGFSHLVEIMPPQGIEFSVKKDIIKVKGFDKEKVGNIAAKIRTIRPPEPYKGK
                     GIRYIDEVVRKKAGKKAVASEK"
     gene            complement(6637..7200)
                     /locus_tag="P5570_01595"
     CDS             complement(6637..7200)
                     /locus_tag="P5570_01595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR39432.1"
                     /translation="MNSLEKFLYAPFGNNIIIGPHIKDQLNFLKGILPEEFKGREMFD
                     LGCGDGKVTVLLAEIFQPKKIFGCDVEESLVKKAKKRGIEARVIDLEKEVPKGELAVI
                     WGVLHHLKDPQKILEKIKNNFQFILIREPLKEQKALHHFKVLSLLEMGDPFEKEKIKR
                     ILDDVLGDYQSFERNGAVMALWSAPKM"
     gene            complement(7293..7694)
                     /gene="rpsH"
                     /locus_tag="P5570_01600"
     CDS             complement(7293..7694)
                     /gene="rpsH"
                     /locus_tag="P5570_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRR39433.1"
                     /translation="MSDPLSDMLIKIKNAGAVNKKTVSIPYSNIKYQLAKILLKEGFV
                     EDIKKKPQSVLHPQGRIVIKLNYDDKGMHKIHQIKRVSKPGRRIYLKAQDLYMPKSGY
                     GLLVVSTSQGLLTGKEARKRKIGGEAICEIW"
     gene            complement(7723..7908)
                     /locus_tag="P5570_01605"
     CDS             complement(7723..7908)
                     /locus_tag="P5570_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRR39434.1"
                     /translation="MAKKCQIIKSQKKPKYTTRKVRRCFRCGRKRGYMREFGLCRICF
                     REMANKGVLPGVKKASW"
     gene            complement(8006..8566)
                     /gene="rplE"
                     /locus_tag="P5570_01610"
     CDS             complement(8006..8566)
                     /gene="rplE"
                     /locus_tag="P5570_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRR39435.1"
                     /translation="MMLSLKEKYNKEVIPLMVKKFGYKSIMAVPKIKKVAVNVGIGKI
                     LESVDPSKRENIIKDISSNLALICGQKPVVTKARKAIAGFKIREGSPVGIKVVLRKDR
                     MYDFLERVINLALPRSRDFQGISQKSIDKKGNLTIGIKEHIVFPEVKPENSKVIFGME
                     VTVVVEAKKREEALELYKLLGFPLKV"
BASE COUNT         2655 a         1699 c         1108 g         3093 t
ORIGIN      
        1 attattttaa attcgtatat tcgtgattgc tccatttttt attttaacat aattttaaag
       61 gaatatcaaa gttttttcaa tgtttttttt aaaacctttt tactagaagg tgtatctatg
      121 tttaatgttt ttcccaattc cactattgct ccaatataat aatcaatctc gtctaggttt
      181 ccttttggct tacctccgcg acccttactt atcatagatc ctatctttga ccgtactgcc
      241 cccaaaacag gcaacggtgt tttttcaatc aaagtagcta aaaaagcaat aggagcgtta
      301 ggtaaattta aaaacctacc gccactggtt tttactacct tgatatactc tcttaacaca
      361 tttatttctt ctacaaaaac atcttttttc ttaaaaccgt cttttataga aagaccctca
      421 acagctgaaa caactccaag cagatttgaa aaaagttttg aaaattccat atttgtaaca
      481 tcctctggca aaaccttttg tgcttctata tgtgcttctt taaaaacctt ttctatcttt
      541 tgtgtatctt ttgatccaga aaatactcca aaggtcaaac gaataggcaa aaaataatta
      601 atgcaaacat tgccaaaatc ttttttagag gacgcttcca ggtcattttt aattattttt
      661 ttttcaattg gatttaaaag gcatactctg ataatttgaa tactttctcc cttctctcca
      721 aaaacctctt tcaatgcagt caatgcttct ttgccagctg caataccgtt ttgcgaaagc
      781 accaaagaag gcaatttttc ttctttgccc tttaaaaaat cacttatttt ttgataatag
      841 ctctttactg gcaaaccaac aggattctta gtggcaagaa aaataatatc cggcaattct
      901 ttgccgggaa attcgtcctc actaatcacc ctgccttgaa ataattctct ctgagaacca
      961 cttttttcta tataaagttt ctttttttta atcaaatcta tttcttcttt tctagcaaca
     1021 cctactacct cagcagcatt ttgaagttcg taagcaaaca ttgttccggt ccttccccca
     1081 accccaatta ccgctacctt tgtttccata gaaatttata ataattaacc attagcattt
     1141 attcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntagc atttattcat
     1261 cagccatttt ctttttaaat tcttccacta gttttatcgg tgttggatca atatttcgag
     1321 aaacagcaag ataatatgag gtccaatcag cgattaaatt attattgaaa attttatcaa
     1381 gaatattttt tccttcaatc tgaacaaaat ttgtttttat atttcttttt tgaaaaattt
     1441 ccgagagaat attcattctt tttataattc gagggtggtc tgttttgtca ctcaaaaaaa
     1501 taacttgaaa taattctctc cattctttaa tctcgcaatc tcccattccg gtcatttcat
     1561 tatggttaag ttcaggtaaa gaattataaa aagatggaat ttttgaattt tcattaaact
     1621 ttattttcca aattctagcc aaacatttat tgtcctctga actataaaca agaggaattt
     1681 ttccttttaa tttttttgcc aagcttaaac ctgttttttc taatttatgg ggggctaaat
     1741 ttttctccag cgcaaataat tccttgtcta agttttttat caatttaaaa ttagataaaa
     1801 ttttaatcaa tgctgaaaat tgataaccca aagcacaacg tggttgattg ccagctgcaa
     1861 tttgagcaaa tggcacattg ttcttttttg caagttctat caattttcca ccagaagcaa
     1921 tagcagcaat agataatttt ttctttcttg cttctttaaa cgcagaaatt gtttcttcgg
     1981 tattgccaga ataagagata cacattacaa aagaattttt attcgtcatt ggatgcaatc
     2041 cataattttg gtgaataaaa accggcaaat tcgttaacaa caaatgatta tttatcatta
     2101 caaaaacatc accaggcaaa gctgacccac ccataccaca aataataata ttagaaaata
     2161 aagagcgatt ggcggatgtt tttatatttt ttgctgcttc aacgcccact ttaaattgtt
     2221 ttggaaaatc caaaattacc tgacgcatat ttgattgatc aaatttttta gccataaatt
     2281 taaatattaa attgggtgag cgaggagatt cgaactccca ctaagggttc cacagaccct
     2341 tgtgcaaagc cactacacta cgctcaccgc tatgtcccca cgaggacttg aaccccgaac
     2401 ctactcctta gaagggagtt gctctatcca gttgagctat ggggactttt tattttaaaa
     2461 agggccccgg gcgggattat cttgaacaaa tccaagtggg tgcccgcacc ttgaagccac
     2521 ggccccaaga aatataggac tcccaatgaa tctattttag tcaagaaagt tccttgtacc
     2581 gggacccaat atattataca tcaagaatga aaaataagtc aaaaaatcaa gttttaagat
     2641 tttatttttt ctgcaatttc ctgaaatacc tcctctacac cttgttcacc atttatttca
     2701 attaattttc cttgcttttt gtaatatttt attactttca tagtttcttt tttatatttt
     2761 tcaattcttt tttttattga ttctatatta tcatcaggac gagtaaacaa ttttcctcca
     2821 cattgatcac aagtttttaa ttttttaaag tcaccggccc aaggaataac tcttccacat
     2881 tttttacact gtctccgttt tgttaaacga ttaaacgatt cttttataga aattttaata
     2941 agaaaaattt taacattctt gtcccattcg taccaattca aagattcatc aatcaaataa
     3001 gcttctaaaa tcttccttgg actcccatca atcacaagcc cattgaaatt ttttttagta
     3061 gattttaatt tttcaaattg atcaagccat aatttaaaaa taacaggcgt aggaactagt
     3121 cctccaacat caatggtttg tctaattttc ttaccagtaa aatctttttc tttttttctt
     3181 tccctcaaaa gatctccgct tcctatataa tctaatttaa atctttcaac aaagaggttt
     3241 gcctgagtac cctttccaga tccaggcaac cctagcatca taataactaa tggattagaa
     3301 tttttcataa gaatttataa ttcgattaaa ttcgcaattc gtagaaatta atcgtattct
     3361 cgcatttcta attgactttt tatttgtttt gatgtttcaa gtataactga aacaataata
     3421 agaagagagg tgccaccaac agcaaaatta aaggtggtaa taccagtaaa cccttgaatg
     3481 atagaaggca taacggcaat aagaccaaga aaaagggcac caataagaag aacacgatta
     3541 agaatatgac tgagaaattg agcagtctgt ttacctggac gaagaccagg aataaaccca
     3601 cccatttttt gaagattatc agcaatgctc tccggatcaa aagtaacagc tgtgtaaaaa
     3661 taggtaaaaa gaacaacaag aataaaataa caaaccccgt atattaattg gttttgaaaa
     3721 agattattga taatattggc aaacccagag acaaaaggaa tggaagtgtc agcaaacatt
     3781 gaaccaaaca ttccaggaat catcattata gataaggcaa aaatgatcgg aatcacacca
     3841 gcgggattaa tcatcattgg aagataggtt tgggcgccac catacatacg acgaccacga
     3901 atacgcttag cataagagac aggcacatta cgtcgcgcct cgtttataat aacaacacca
     3961 acaataatga taatagaagc agcaaggaaa agaagatatg atggtaggtg ggaaggatcc
     4021 caagtcataa tagtttgacc aatttgtgca ggaacgctag aaataatacc agcaaaaata
     4081 agaagagaaa taccattgcc aatgcccttc tcagtaataa gttcaccaat ccacattaaa
     4141 aaaacacaac cagcagtgat ggtgataatt gaattgacaa gagacgatgt agtaaattgt
     4201 cccaaagcac cctggctttg caataagcga agcattgcaa aggactgaag agcagccaat
     4261 ggaacagtaa gaattcgccc atattgagta aattttcttt tcccctcttc ccctgattct
     4321 tgatacattt cttttaattt tggaaaaatc aaggtcagca gttgcataat aatggttgct
     4381 gtaatatatg gacccaaacc aagcattaca atagaaagat tttcaagtgt tcctcctgta
     4441 aaaacattca ttaatccaaa aaattgattg gtctcaaaaa aagaagcaag ggcatctcta
     4501 tcaacgcctg gaatgggaat attagcagcc attcgaaaaa ccaccatcat tgccaaaaca
     4561 aataggacct tactcctaag gtcacggtat ttaaaaatag cgataatctt actaaaatcc
     4621 ataatataat tataaggttt gctttgttcg taatattaac ccttttttaa cttcctttcc
     4681 ttccttaaat ccttagatga tgaaaaatat gagttaatat ttatgaatta agtaactaac
     4741 agctattaag tccatcaaaa gaaaactgtt tgctattttc ctcttttttt aggaattttt
     4801 ttaataaaat cacgaatttc tggtcttatt tttgctccag cccgtgattt ttgacctttc
     4861 attcctttac cagaataagt tcctcgtttc cctccgcggc caacacgttt tttagttttt
     4921 ggtttatgaa taggttgaac ttcgtgaagt tgcataaaaa tggaaatttt aatctataaa
     4981 caacatcaaa tactccaagt gtttggataa ttcggttaat aattcaaatc acttggatcg
     5041 catttcaatc tcttggaatg ctttcaatgt tgctcttgca atatttattt tgttatttgt
     5101 tttggaaatc aattttcctg taatattggg cacccctgct aattcacata ctgttctcat
     5161 tgctcctccc actacaagac ctgagccttt tcgttgagga cgcaaaaaaa ccctggctga
     5221 ttgatatttc acctcaatat cataaggtat tgtttcgttt tttaaaggca cagaaatcaa
     5281 agattttttt gcttttgtag tagctttctc tactgcctcg cttacatctt ttccttttgc
     5341 tacaccgatt cctacttttc cagctcgatt tcccactaca actgttgctc gaaaacgaag
     5401 acgcctgcct ccggcagtta ctcgagtaac ccttgccaaa tctaataatt gtgattcaaa
     5461 ttcatcttta ttttgcataa aattattatt ataattctga tctgtaaatg gtattcaaat
     5521 gctaatatta tttagatgta cgttacgatg tacgttgcaa agtaagcaaa gcaaacttaa
     5581 gttgtccaaa cactagaact gcaatccgcc ttttcttgct ccttcagcta atgcttttac
     5641 tcttccatga tatcgatagt accctctatc aaatacaacc ttttctatgc ccttctcttt
     5701 gccttttttt gcaatcaatt ttcccacttc aaaagcaata tcagtttttt ttcctttttc
     5761 ttttatctct ttgtcatcag cacttataat tgtctttcct tcattatcgt ttattaattg
     5821 acacgatata tgagtattgg aacgaaaaac tgaaagccga ggacgcatat ttgttccttt
     5881 aatttttgct cttactcttt tatgtcgacg atctcttttc tttttaatat ctttttgaaa
     5941 atttttcatt tttatataaa ttaaaaataa aaagctaatt atttttctga agccactgct
     6001 tttttacctg ctttctttct taccacctcg tcaatataac gaatgccctt gcccttatat
     6061 ggttccggtg gacgaattgt tctgattttg gcagcgatat taccaacctt ttctttatca
     6121 aagcccttta cctttattat atctttttta acagaaaatt ctataccttg aggaggcatt
     6181 atttcaacca aatgagaaaa tcctattttc aaaaccaatt tatttccttt tagttccact
     6241 ctccaaccca ctccttccac ttttaattct ttctcaaact ctttatttac acctattaca
     6301 gcatttttaa tatgcctctg ccaagtaccc cacaatgcct ttttggactt atctctactg
     6361 cccttgaatc tcaccaatat ctcattccct tttttttcaa cttcaatctc tggtggcaag
     6421 acatacttca tctttccttt tggtccttca ataaaaatct catttccttt tatttccact
     6481 tttgtccctt ctaaaatttg aattgattgt ttaccaattt tagacataat tataaaataa
     6541 ttccaacacc atttaaatca tttaaatgat gttgcttata agaactacat ttacgttatt
     6601 tgtatgtgcg ttttcgaata aaacttataa agcaaatcac atttttggag cactccaaag
     6661 cgccattacc gcgccatttc tttcaaagct ttgatagtca cccaaaacat cgtctaagat
     6721 tctttttatt ttttcttttt caaaaggatc tcccatttcc aataaactta aaaccttaaa
     6781 atgatgcaat gctttctgct ctttcaatgg ttctcttatt aaaataaatt gaaaattatt
     6841 ttttatcttc tctaatattt tttgggggtc tttgaggtga tgaagtactc cccatattac
     6901 tgctaattct ccttttggaa cttctttctc aagatcaata actctagctt caattccccg
     6961 tttttttgct ttttttacta aactttcttc aacatcacaa ccaaaaattt ttttgggttg
     7021 aaatatttca gctaataaaa ctgtgacttt tccatcacca catccaagat caaacatttc
     7081 ccgtccctta aattcctctg gtaaaattcc ttttaaaaaa tttaattgat ctttaatgtg
     7141 gggaccaata ataatattat tgccaaaggg agcataaaga aatttttcaa gactattcat
     7201 aaaagtacat tttatccgaa tctactaatg acttatcaaa tctaataatt ttcattagag
     7261 tgtattcaga cagttattcg tatttctatc tactaccata tttcgcatat tgcttcaccc
     7321 cctatctttc ttttccttgc ttccttccca gttaaaagac cttgagacgt tgatacaacc
     7381 aataatccat atcctgattt tggcatatat aaatcttgtg cttttaaata aatcctccgc
     7441 ccgggctttg aaactctttt tatttgatgg atcttgtgca ttcctttatc atcataattc
     7501 aacttaataa caattcttcc ttggggatgc aaaacagact gaggtttttt ctttatgtct
     7561 tctacaaacc cttcttttaa taaaatcttc gctaattgat atttaatatt ggaataagga
     7621 atactcactg tctttttatt aacagcccca gcatttttaa tttttattaa catatctgaa
     7681 agtggatcac tcatataagt tgataaatat ttattttatt ttttaccatg atgctttttt
     7741 cactcctggc aatactcctt tgtttgccat ttctcgaaaa caaattcgac aaagaccaaa
     7801 ttccctcata tatcctcttt tcctgccaca tctaaaacac cgtctcactt tacgagtggt
     7861 atattttggt tttttttgac ttttaataat ttgacatttc tttgccataa tgctttttct
     7921 tcgaaaaata atttctaatt tttaatttct aattttaaaa cagttttgat ttttgaatta
     7981 taatttcggg tttaatgctt agattttata ctttcagtgg gaatcctaaa agtttatata
     8041 attctaatgc ctcttctctc ttctttgcct caaccactac tgttacttcc atcccaaaaa
     8101 ttacttttga gttttctggt tttacctctg gaaaaactat atgctctttt attcctattg
     8161 tcaaatttcc ttttttatca atggattttt gggatattcc ctgaaaatca cgagatcggg
     8221 gcaaggccaa attaatcact ctctctaaaa agtcatacat tcggtccttt cttaaaacta
     8281 ccttaatacc cacaggggac ccttctctta ttttaaaacc cgcgatcgct tttcgcgcct
     8341 tagtaacaac aggcttttga ccacaaatta aagccaggtt tgaagaaata tcttttatga
     8401 tattttctct tttcgatgga tcaacacttt ctaaaatttt tccaattcca acattaactg
     8461 ccactttttt aattttgggc acagccataa tacttttata tccaaacttt ttcaccatca
     8521 aaggtataac ttctttatta tatttttctt ttaaagataa cataataaaa aatcaaaatt
     8581 tatagatact tgtgccttcg gcacgatatt gatagatact tgctcgcttc gctcgcgata
     8641 ttcattgtaa caata
//
LOCUS       DAOTQD010000023         8378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_7636_length_8378_cov_7.711763, whole genome shotgun sequence.
ACCESSION   DAOTQD010000023 DAOTQD010000000
VERSION     DAOTQD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7636_length_8378_cov_7.711763"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..2989)
                     /gene="clpB"
                     /locus_tag="P5570_01615"
     CDS             complement(350..2989)
                     /gene="clpB"
                     /locus_tag="P5570_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRR39436.1"
                     /translation="MNGDKFTIKAQEALQRAQEIASEKQHQQIDALHLLYALLDDQES
                     LVNVILKRIGKDSYLIKEKVNQVLLSLPRVFGQTSFGQAYLTQDFAKVLEQAKRECQK
                     MGDEFISVEHLFLAILATQNRAREVLADVNYDQVLKILVDVRGGEKVTDQSPESKYEV
                     VKKYTRNLTEEARQKKLDPVIGREEEIRRVIQVISRRTKNNPVLIGEAGVGKTAIVEG
                     LAQRIVAGDVPESLKDKEIISLDLGAMIAGTKYRGEFEDRLKALLREINRSKGKYILF
                     IDELHTVVGAGAAEGAIDASNLLKPALARGELRTIGATTLKEYQKYIEKDPAFERRFQ
                     PVYVSEPSVEDTIAILRGLKEKYELHHGVKIKDSALVAAVKLSHRYIGDRFLPDKAVD
                     LIDEAASSLRLEVESEPVELDKIKREMMKLQIEKEALKKEKDPQSKERLAGIEKELAE
                     LQEKRDKIEIQWKKEKEIIDKIKFLKKDLEKARQEAEMAEREGDLQKVAEIRYGRIPQ
                     NEKQLKKLEKELKKFQEGGRRILKEEVTEEDIARVVSRWTGIPVSRMLEEESQKLARM
                     EEELHKRIIDQEEAVKAVSNAIRRSRAGVSEELRPMGVFLFLGPTGVGKTELARALAE
                     FLFNDENALIRLDMSEYMEKHTVSKLIGSPPGYVGYEEGGQLTEKIRRRPYSVVLFDE
                     IEKAHPDVFNILLQVFDDGRLTDAKGRVVNFKNTILIMTSNIGSEEIARSKNLGFETK
                     EEGQQKQKNNSKQDLKRKIEEKLREYFKPEFLNRIDEIIIFDFLSQKEIEKIVDLQLT
                     KIQKRLSERRIKITVTPKAKKYLSKVGFDPNLGARPLKRTIQNLVLNPLAQMIIESKI
                     KPEDRVKIDEKDGKIIITPMNKK"
     gene            complement(3088..4245)
                     /locus_tag="P5570_01620"
     CDS             complement(3088..4245)
                     /locus_tag="P5570_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRR39437.1"
                     /translation="MKPYSIIIASVILSFVVGFIGGIMGIKYYPQITTIFSEESTTVS
                     NTTGQNTPTIIEKEVTSSQEQQVIKVVDEALPSVVSILAEGEIAVPGLPFFYQQIETG
                     GTGFIISSDGLILTNKHVVQYDNIDYTVILSNGDQYQAEVLARDNTQDLAVLKIEPKK
                     GESFNILSLGDSDNLKVGQTVIAIGNALGEFQNSVSVGVVSGLARSITAAGTGVAETL
                     DQLIQTDAAINSGNSGGPLLNLSGEIIGINTAVASGAENIGFAIPINQAKKAIESVKT
                     TGKITYPFLGVRYILINQEIKELYNLSVDYGAWIIRGSGYNDVAVVANSAADKAGIKE
                     NDIILEIDGEKITTNNTLSKVILSHNVGDKIKLKILREGKEIEIEAVLEGQ"
     gene            4373..4798
                     /gene="rnpB"
                     /locus_tag="P5570_01625"
     ncRNA           4373..4798
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5570_01625"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4793..5722)
                     /locus_tag="P5570_01630"
     CDS             complement(4793..5722)
                     /locus_tag="P5570_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39438.1"
                     /translation="MLSSLEKSILKSIVYFDILSYPLKEKELLWNLANTFDVTSRDYF
                     LSVSLGEMIKKSQYLQEVIRNNNGFYFLKGREELIEERKEKERISEQNWKKLKKITPI
                     INWTPFLKGVFVSGSLAINNSNKSSDIDLMIIAEKGRIFTVRFFLTLFLSIIRERRTP
                     HKIAGKICLNHYLADNALRVNFPSVYNAYTYLHLKPIISREGVFERFRKEQDWMKDYI
                     LFWDKIFKPPFQIEKKSKTAVILEKKLSGSLGDTIEEKLKNIQLKRKEKNYPQGIKNG
                     RVILNDYLIELHPDSPEKEILKRYQKKLKELLF"
     gene            complement(5737..7335)
                     /locus_tag="P5570_01635"
     CDS             complement(5737..7335)
                     /locus_tag="P5570_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39439.1"
                     /translation="MSQQIFYIDPLDDLNKARELLKKSSKNKVVLVLPEENTILKNIE
                     NLTILKKEAQNLGKRLAVFSSDPQYKKLAEDCGIEIESSLRLADAENKEIEKEQYKKE
                     VFSKPRVSDILPPQKAEPVQPSISKKIETKDVEKSQELPIPQKATKKWVGPLLYTIFF
                     LLLVGGAVFSFTFLPRATITIVPVSEEIDFSGKFEVKKGADIDLEANIVPGTIIEKSK
                     NIEKSFLATKTEDKEEKARGKITIYNEDTSSHRFVPGTRFKSQDGKIFKSQDWINIPA
                     GSKTNPGQIEIEVVAESAGEEYNIEPTTFTVPGLSGTNLFDLIYAKSSEAMSGGFVGK
                     TKVVGEEDIEKAKKEIEAMQDNVIDQLKKEILKEISPDLQSFFEDLIQVEKEEIVFDK
                     KAGDIVETFKASTKVTVWVLSFEEDDVQEIIAKVIAEEIKEGVDFEEVVSSQKIDYEV
                     IENNMESGVLKISFEGKEKVAWKVTADQIKKAVTGMDQNAFQKYISEDMKGKIKDGEV
                     ELWPLWVKEIPQREDRIFVEIKYE"
     gene            complement(7513..8070)
                     /locus_tag="P5570_01640"
     CDS             complement(7513..8070)
                     /locus_tag="P5570_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39440.1"
                     /translation="MLEPFLAINFTPEVIELFSVENRKGNLRVLDSLKKNYKDQFSKD
                     IEWELFDLVLTMEKRLGVYFKSAIIGIKKGPVKKIISKNKFLRKYPQKPIRENEFKKM
                     IEECQRESFLKLKKETNDPFHFILISAKIKEVTIDSKKVFNPIGLIGKIISLSITNIY
                     LPIDFYQALKKVFSELNIQTSFEID"
BASE COUNT         2511 a         1588 c         1112 g         3167 t
ORIGIN      
        1 agatttcatc ttcttttcaa cgcgaaaccc gctgtggcgg gttcgcgtga ccgagtgtgg
       61 cgatcccgtg gcacgtctac aaacatacaa taggattact ttctttctgt tttctgattt
      121 ctgttttctt tactgttttc tgatttctga caactgattt ctgtttagta tttggaattt
      181 tgaatttaga atttgtttgg aatttggagt ttaatagttt acgcgcaacg ccaacgttta
      241 ccgtaagacg cattctattt cacgaatacg gatttaaggg tttagaaatt agaaattaga
      301 aattaattaa atgtgttgta ttaaattttt gaatctaaat aagttgattt tattttttat
      361 tcattggtgt aataataatt tttccatctt tttcatcaat ctttacccga tcttctggct
      421 taattttgct ttcaattatc atttgagcta aaggatttaa aaccaggttt tgaatggttc
      481 gcttcaaggg tctagcacca agatttgggt caaaaccaac ctttgaaaga tacttttttg
      541 cctttggagt aacagtaatt ttgatacgtc tctcgcttaa tcgtttttga atctttgtta
      601 gctgcaaatc tactattttt tcaatttctt tttgtgataa aaagtcgaaa ataataattt
      661 catctatcct gtttaaaaat tcaggcttaa agtattctct taatttttct tcaatctttc
      721 tttttaaatc ttgttttgaa ttattttttt gtttttgctg gccttcttct ttcgtttcaa
      781 aaccaaggtt ttttgaacga gcaatctcct cgcttccaat gtttgaagtc attattaaaa
      841 tagtattttt aaaattaact acccttccct tggcatcagt taatcgtccg tcatcaaaca
      901 cttgaagtaa aatattaaac acatcgggat gagctttctc aatttcatca aacaatacta
      961 cgctataagg acgacggcga attttttctg ttaattgtcc accttcttca tatcctacat
     1021 agcccggcgg agaaccaatc aattttgaaa ctgtatgttt ctccatatac tctgacatat
     1081 ctaatcgaat taaagcattt tcatcattaa acaaaaactc agccaatgct cttgctagtt
     1141 ctgttttacc aacacctgtt ggaccaagga ataaaaatac acccattggt cttaattctt
     1201 ctgacacacc agcgcgtgaa cgacgaatag cattagaaac tgcttttact gcttcctctt
     1261 gatcaataat tcttttgtgc aattcttctt ccatgcgagc caatttttga ctttcctctt
     1321 ctaacattcg agatactgga ataccagtcc agcgtgatac tacccgagca atatcttcct
     1381 cggttacttc ttcttttaaa attctccgac ccccttcttg aaactttttt aattcttttt
     1441 ctaatttttt taactgtttt tcattttgag gaattctccc atagcgaatc tctgctactt
     1501 tttgaagatc cccttctctt tcagccattt ctgcttcttg tctagctttt tctaaatctt
     1561 ttttaagaaa tttaatttta tcaataattt ctttctcttt tttccattga atttcaatct
     1621 tatctctttt ttcttgtaat tcagccaatt ccttttcaat ccctgctaag cgttctttag
     1681 attgtgggtc tttttctttt ttgagagctt ctttctctat ctgaagtttc atcatttctc
     1741 ttttgatttt atctaattca actggttctg attcaacttc aagacgcaag gaagaagctg
     1801 cttcatctat caaatcaaca gctttatcag gtaaaaacct atctccgata taacgatgag
     1861 aaagtttcac tgctgcaact aatgctgaat cttttatttt tactccgtga tgaagctcat
     1921 atttttcctt taatccgcgc agaattgcaa tggtgtcttc tactgatggc tctgatacat
     1981 atactggttg aaatcttctt tcaaatgccg gatctttctc aatatatttt tgatattcct
     2041 ttaaagtggt agcaccaata gttcttaact cgccccgagc aagggctggt tttaaaaggt
     2101 tggaagcatc aattgctccc tctgctgcac ctgctcccac tacagtatga agttcatcaa
     2161 taaataaaat atatttacct ttacttctat ttatttcacg caataaggct tttaatcgat
     2221 cttcaaattc tccacgatat ttggtaccag ctatcattgc tccaagatct aaagagataa
     2281 tttctttatc ttttaaactt tctggaacat cgccggctac aattctctga gccagtcctt
     2341 ctacaatagc tgtttttcct actcctgctt ctccaattaa tacaggatta ttttttgtac
     2401 ggcgtgaaat tacttgaatt actcttcgaa tttcttcttc tcttccaatt actggatcta
     2461 attttttttg tcttgcctct tcagtaagat tgcgcgtata ttttttaacg acctcgtatt
     2521 ttgattctgg actttgatca gttacttttt ctcctcctct cacatcaact aaaattttta
     2581 gcacctgatc ataattaaca tctgccaaaa cctctcgtgc tcgattctga gtagctaaaa
     2641 ttgctaaaaa taaatgctcc acggaaataa actcatctcc cattttctga cattctcttt
     2701 ttgcctgctc aagtaccttt gcaaaatcct gagtaaggta tgcttgacca aaagaagttt
     2761 gcccgaaaac acgaggcaaa gataacaata cttgattgac tttttctttt atcaaataag
     2821 aatccttacc tattcttttc aatattacat taactaaact ttcttgatcg tctaaaagag
     2881 cgtaaagcaa atgtagggca tcgatctgtt gatgctgttt ttcactagcg atttcttgag
     2941 ccctttgaag agcttcttga gcttttattg taaacttgtc accattcata aattatttta
     3001 tttagttttt atcaagttgt aacaaatgtt tcttgaatac tcgaaaaatt aatttgttaa
     3061 ttagtaaatt atttaaaaga taaattacta ttgtccttcc aatactgctt caatctctat
     3121 ttcttttccc tctcgcaata tttttaattt aattttatca cctacattat gagaaagaat
     3181 gacctttgaa agcgtattat tagtagtaat tttttcacca tcaatctcta aaataatatc
     3241 attttcttta atccctgcct tgtctgcagc actattagcc accactgcca catcattata
     3301 tccacttcct cttattatcc aagcaccata atccactgat agattatata gttcttttat
     3361 ctcttgattt attaaaatgt atcttacgcc taaaaaagga taggtaattt ttcctgttgt
     3421 ttttacactt tcaattgctt ttttagcttg attaatcgga attgcaaaac caatattctc
     3481 agcgcctgac gctacggctg tatttattcc aataatttca ccagataaat ttaaaagcgg
     3541 cccacccgaa tttcctgaat taatagcagc atcggtttga attaattgat ccaacgtttc
     3601 cgctactcct gtaccagcag cagtaatgct tcttgctaag ccagaaacca cacccactga
     3661 cacactattt tgaaattcac ccaaggcatt accaatagca ataacagttt gtcccacttt
     3721 taaattatca gaatcaccca aagatagtat attaaaagat tcgccttttt tgggctcaat
     3781 ttttaatact gccaaatctt gagtattgtc tcttgctaat acttctgcct gatactgatc
     3841 cccattagac aaaattactg tataatcaat attgtcatac tgtacaacat gtttattggt
     3901 taaaatcaat ccatcagaag aaataataaa accagtacca cctgtttcaa tctgttgata
     3961 gaaaaatggt aaaccaggca cagcaatttc tccctcagcc aaaatggaaa caacactggg
     4021 caaagcttca tctactactt ttattacttg ctgctcctga ctgcttgtca cttctttttc
     4081 aataattgtt ggagtgtttt gacctgttgt attagaaaca gtagtgcttt cttcactaaa
     4141 tatcgttgtt atttgaggat aatattttat ccccataatt ccacctatga atcccacaac
     4201 aaaggaaaga ataactgatg caataataat agaataaggt ttcatatgtg tatagtttaa
     4261 aacagaatat atcaaatttt aaaaactttg taaaggactt actaaagtgc aaaaagtgtg
     4321 tatacattac cacaaggcct tgactttttt tcaaatttaa ggtaaaataa aagaaggtag
     4381 atgagatagt cactcttttc aataaaatta aaatttgaaa agagaggaaa gtctgagcac
     4441 cctccgattt caccctctcc cctattaaat tgaggaaaat tggagaagtg ggcagtgggt
     4501 aacacccacc gtttgtttaa ttttgtttta caaattaaat aaatagggaa agtgccacag
     4561 agacaatact acccctcatc ttcataaaag atgaggggga aaggtgaaaa gttctgctta
     4621 aagcagattg actaatggag acattagtta tggcaaaccc tgcctggtgc aagttgtcct
     4681 ttgggaaaac gcttgacccc tcaagcaatt gaggggctag aaaaatgact atcaccactc
     4741 tcgcttttta caaaaacgaa gggtggtaca gaactcagct tactatctac ctttaaaata
     4801 gtaattcttt aagtttcttt tgatatcttt ttaaaatttc tttttctgga gaatctggat
     4861 gaagttcaat aagataatca tttaaaatta ccctaccatt ttttattcct tgagggtaat
     4921 ttttttcttt tctttttagt tgtatattct taagtttttc ttctattgta tctcctaatg
     4981 agcctgataa ttttttttct aatatcacag cggtttttga ttttttttct atttggaaag
     5041 gcggtttaaa aattttatcc caaaacaaaa tataatcttt catccagtct tgctcctttc
     5101 taaatctttc aaaaacacct tcccgtgaaa taatgggttt taaatgcaaa taagtataag
     5161 cattataaac actgggaaaa tttaccctca aagcgttatc tgctaaataa tgatttaaac
     5221 aaattttacc tgcaatttta tggggagtcc ggcgttctct tattattgaa agaaaaagtg
     5281 ttaaaaaaaa cctgacagta aaaattcttc ctttttctgc aataatcatt aaatcaatat
     5341 ctgatgattt attagaatta tttattgcca atgatccaga aacaaaaacc ccctttaaaa
     5401 acggggtcca atttattatt ggggttattt tttttaattt tttccaattt tgttctgata
     5461 ttctttcttt ctcttttctc tcttctatta gttcttctct accctttaaa aaatagaagc
     5521 cattattgtt tcttataact tcctgtaaat actgactttt ttttatcatt tcaccaagag
     5581 aaacgctcaa aaaataatcg cgcgacgtta catcaaatgt atttgccaaa ttccataata
     5641 attctttttc ttttaacgga tacgataaaa tatcaaaata aacaatgctt tttaaaatag
     5701 atttttctaa tgatgagagc acaaattttt aattttttat tcgtatttta tttccacaaa
     5761 gatccgatcc tctcgctgag gaatttcctt tacccacaaa ggccataatt ctacttcgcc
     5821 atctttaatt tttcccttca tatcttcact aatatatttt tggaaagcat tctgatccat
     5881 tcctgttact gcttttttta tttgatctgc tgttactttc caagccactt tttcctttcc
     5941 ctcaaagcta atttttaaaa caccactttc catattattt tctataacct cataatctat
     6001 tttctgacta gaaaccactt cttcaaaatc aacaccttct tttatttctt cggcaattac
     6061 tttagcaata atttcttgaa catcgtcttc ttcaaaactt aatacccaca ctgttacttt
     6121 tgtgcttgct ttaaatgttt caactatatc acctgctttt ttatcaaaaa caatttcctc
     6181 tttttccact tgtattaaat cttcaaaaaa agattgaagg tcaggagaaa tttcttttaa
     6241 aatctctttt ttcagttgat ctattacatt atcttgcatc gcttctattt ctttttttgc
     6301 tttttctata tcctcttctc caaccacttt tgtcttgcca acaaaaccgc cactcattgc
     6361 ctcacttgat ttagcataaa ttaaatcaaa aaggtttgta ccagataaac caggaacagt
     6421 aaaggttgtg ggttcgatgt tatattcctc tccggcactc tcagccacta cttctatttc
     6481 tatttgtcct ggatttgttt tgctacctgc tggaatatta atccagtcct gacttttaaa
     6541 aatttttcca tcttgagatt taaaacgagt accaggtaca aaacgatgag aagaagtgtc
     6601 ttcattgtaa atagtaattt tgcctctagc tttttcttct ttatcttcgg tcttggttgc
     6661 caaaaaactt ttttctatgt tttttgattt ttctattata gttccaggaa caatattcgc
     6721 ttcaagatca atgtcagcgc ccttttttac ttcaaacttg ccagaaaaat ctatttcttc
     6781 gcttacaggc acaatagtaa ttgtggcacg cggtaaaaag gtaaaagaaa aaacagcacc
     6841 accaaccagc aataagaaga atattgtata caacaatggt ccaacccatt ttttggtggc
     6901 tttttgagga ataggtaatt cttgagattt ttcaacatcc tttgtttcga ttttttttga
     6961 aatggatggc tgaactggtt cagctttttg agggggtaaa atatcactta cccttggttt
     7021 tgaaaatact tcttttttat attgctcttt ttctatttct ttattctctg catctgctag
     7081 tcttaaagaa ctctcaattt ctattccgca atcctctgcc aattttttat attgagggtc
     7141 gctggaaaaa acagccaatc gttttcctaa attctgtgct tcttttttta agatagtgag
     7201 gttttcaata tttttaagaa tggtgttctc ttcaggcaat accaaaacca ctttattttt
     7261 tgaacttttt tttaataatt cccgcgcttt atttaaatca tctaacggat caatataaaa
     7321 aatttgctga gacatatata ataaatatta aaaaactccg aatatgataa gataaattaa
     7381 tttaaattta caaaattgtt catttattta acaaacgaac ttaaattttt tataaagttc
     7441 ttttgggcaa tactcaatat atttttgttg tttaaatttt aaaacaaacc taaggttaat
     7501 ataccaaaaa tttcaatcaa tttcaaagga agtctgaata ttaagttcag aaaaaacctt
     7561 ttttaatgct tgataaaaat caattggcaa ataaatgttg gttatagata aagaaataat
     7621 ttttcctatt aaaccaatag gattaaatac cttttttgaa tcaattgtaa cttcctttat
     7681 tttagcacta attaaaataa aatggaaagg gtcatttgtt tcttttttta attttaaaaa
     7741 tgattctctt tgacactctt ctatcatttt tttaaattca ttttctctta tgggtttttg
     7801 agggtatttt cgcaaaaatt tatttttgct gataattttt ttaacaggcc ctttttttat
     7861 accaataatc gcagatttaa aataaacacc taatcttttt tccattgtca aaaccaaatc
     7921 aaacaattcc cattctatat cctttgaaaa ttgatctttg taattttttt tcaaggagtc
     7981 aagcactcta agattacctt ttctgttttc cacagaaaat aattctatca cctcaggagt
     8041 aaaatttatt gcaagaaatg gttctaacat agctttgaat tattattttt tattaaacaa
     8101 tcaacgcaac ataacttttt aaaaaccggg gatggtttgg aatttctcaa ctcgaatcaa
     8161 aaataacagt tcctgaactt atcaaaaaat tacaatagaa atagatactt actaaatcca
     8221 atcaaaggtg cttttaaaat aaggatcgtt taacaatccc aaggaataag ataaggtaga
     8281 ttcaagtttt taacgcaaca atgtcacggg atcgccacgt cgttcacgcg agccccgtaa
     8341 gaaatctttg atttcgcacg gggcgaagcg ttgaaaag
//
LOCUS       DAOTQD010000024         8327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_7690_length_8327_cov_7.665498, whole genome shotgun sequence.
ACCESSION   DAOTQD010000024 DAOTQD010000000
VERSION     DAOTQD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7690_length_8327_cov_7.665498"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1588)
                     /locus_tag="P5570_01645"
     CDS             complement(116..1588)
                     /locus_tag="P5570_01645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRR39441.1"
                     /translation="MFHSFSKKIIVLTLIFTLVTSLFLPLLPVGVFAEDSDFCNQVKN
                     ATCDDDLKSLSEDSRLKDCGDEILNYFQKKVKDCNAKLEAQKNQTQKQLNSLNSQQTT
                     AQKTLNNLTFNIKKLNTEIASLNLSLAELENAIQEREKAIKELDDALGQHKAILSEAI
                     RQIYEYNTLSYIEMLLGYRTLSDFNQKLEEIDRLQKSLKSSMEEIRKAKEQMEKEKEE
                     LTQKKKEQEQYKAMREMSKQSLALRQEQQQYLIEKLAVAKTPLEKEMARIEAELMELR
                     TAMSQIERYLAQWVLVGQVNWSTIFSAVQRASSATGVRPALLLGILQIESRFGTGLGT
                     PGRYQDYCNWSSSPYCAYSTEAAALESICQKFGYKPTEVPMSSACAIGPSQFLPCTWL
                     AYGGGGNPWNLNDAVMAMARKLNASGGAEENKVDAYNPCKKCAEGFVNGIKVSNPSYV
                     NSVITSADAWQDVIDICGLNLSCPQMQQRLQTKFSNIPTE"
     gene            complement(1648..2109)
                     /locus_tag="P5570_01650"
     CDS             complement(1648..2109)
                     /locus_tag="P5570_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR39442.1"
                     /translation="MTKMQKAKIKMQFNETIVSFHTSFFTKVKNSANDKSKFKEDFNK
                     RLVKFSLEILQFSDKLRKDRLFWPIVDQLIRSATSIGANVIEAKSSSSKKDYIKFFEI
                     ALKSANETKYWLILIRESRKELKDKVNNLLLEVNQISKVIASSILTLKGKK"
     gene            complement(2102..3229)
                     /gene="rodA"
                     /locus_tag="P5570_01655"
     CDS             complement(2102..3229)
                     /gene="rodA"
                     /locus_tag="P5570_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRR39443.1"
                     /translation="MAISSNIFLKKFDWLFLIFAVLLTILGLVCLYGLSLFSGDFSNF
                     NQQAIFFLIGIILAISLSFIDFKALRDSSLFILAIYFFSIILLLGVFFLGKEIRGSRS
                     WYHFGSFTFQPVEIVKIIFILFFAKYFSQRHVEMYQKRHIVLSFVYAFIPTALVFLQP
                     DFGSAIILILIWFSMMLVSGIKRKHLLIIFGVGVLFFLVAWNFVFTQEQKDRFTTFLE
                     PYINPEGEYIDPQGTGYHIQQSIIAVGSGGLFGKGISEPYTQAKLGFLPEAHNDFIFA
                     AFTEMFGLIGVLVLFLLFGLFFWRGIEIAKKSNNNFSRLLVSGFIILVGAEAFINIGM
                     NIGLLPISGLPLPFLSYGGSSLLSLFIGIGIIESVKVHGND"
     gene            complement(3234..3674)
                     /locus_tag="P5570_01660"
     CDS             complement(3234..3674)
                     /locus_tag="P5570_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39444.1"
                     /translation="MLTPFGRKIKQINIALGFLTFCLGIAALVWFFVAEPTSIEGEEI
                     KGLEIKDVFAEFQSDDQPSDPPFTYKVSVNIENPNDKFIAKEVAFKIEIKDENGKVIF
                     QKGETTEVKGNEQKKIEKEISIDEQGKNVSFKIVKIKWEEKEND"
     gene            complement(3774..5426)
                     /locus_tag="P5570_01665"
     CDS             complement(3774..5426)
                     /locus_tag="P5570_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39445.1"
                     /translation="MNLHWTKKKTIILVFVLLVALIGTFVYFFFFKNTFSKENIILKI
                     SGPQDIVAGKEISWVVSIKNQSEITIENLELIFEYPEGVFDNEGSVKKKEKRIIKKIA
                     GGEEKTETFSGLIFGVKEETKTAKASLTYNPEGLSAFFQNDTSFSTRISDTSIVFSMD
                     APLKVEPGEKVSISFFWQSSFPFPLKNVQIRFSPPEGFERISNKTEEERQESGKIIFD
                     IGSVNENEGGKRQIEGLLNGEVGDEKLFKIEFGIFDEKLYEFIPLASIEKIVKIKTST
                     LDASIKVNGETNYVASPGENLAFIVDFMNTGEDIYRGLSLTVELESDVLDFSTLRAPG
                     GKIEGKKITFSSDNFPDLLFLGPYGSGSVGFTINLKDYDESFYKENAVIKEIIKIGTI
                     EKSFQTKVSSKLSFKEKIYSKSEFLPQDVRSIFDINISSIIIGKENNFVIGFEIKNYG
                     NQLTDVKITGTLPANVSFLNKFFPSNTDIQFNENKRELVLNIGNISANFKKEYFLQLK
                     IVPSNMPDVIHNNFKLTGKDSWTDKNFEIIIPSQTTQALISE"
     gene            complement(5470..6321)
                     /gene="recO"
                     /locus_tag="P5570_01670"
     CDS             complement(5470..6321)
                     /gene="recO"
                     /locus_tag="P5570_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRR39446.1"
                     /translation="MEYQTMKTNAIVLKEKELGETDKIFTLYTKNFGKFEVMGKGIRK
                     IKAKLKFGLQGLNNVSLEFIRGKNFFIATDVFLKNGFWSLKREIRKYRAGLYVCDITD
                     RLITGEEKDEEIWDLLFETLERIDILPSVNYQLSMAVRYFEWNLLSFLGFEPELYHCI
                     ICQNKLSGGEFYFSAKEGGIVCNKCQMSSRAKRRKAEDKAGSHRSLNADLNESEAGRE
                     IKTNYLSSAKCQKISRDAIKILRLIILKDKNTLKRLKINPVQERELKDLSKYYLSWIL
                     EEENFVI"
     gene            complement(6331..7395)
                     /locus_tag="P5570_01675"
     CDS             complement(6331..7395)
                     /locus_tag="P5570_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39447.1"
                     /translation="MSPLAVNIIKIFSLTIFASVLAILATPALTNFLYRNKLWRKSSR
                     TKTIDGKEAEVFNKFHKKKEVSVPRFGGVLIWGSVLVTALIFWSLSQFFPDNFWLQKL
                     NFFSRSQTWLPMFALVAAALLGLVDDILQVRSRGKYIAGGLTFWQRFLVVLLIGLVGG
                     LWFYFKLDWQTIHIPSNGDAFIGPFYILLFIIVTLACWSGGVIDGLDGLTGGAFASMF
                     AAFGVIALAQEQIDLAAFCGVITGTVLSFLWFNVPPARFYMGETGSLALTTVLAVVAF
                     LTDSVLVLPIIGGLLVMESGSVILQLISKKFFKKKIFLATPIHHHFQAKGWSEAKIVM
                     RFWIIGVVLAIIGIAIRLLG"
     gene            complement(7452..>8327)
                     /locus_tag="P5570_01680"
     CDS             complement(7452..>8327)
                     /locus_tag="P5570_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRR39448.1"
                     /translation="QTINAAQVSKLLTEEEKNNISFSSFFPIKEEDFWTPQLGEKAGL
                     VVLFSPYGKENILYEKNIEEQLPIASLTKIMTAMVVIDNYPLDKEVEISEKAIKTLGK
                     SSLCSGEKMTVENLLNLALLVSSNDATSALAEIMGEERFVKSMNEKAKEIGLLNTFFV
                     NPHGLDEDDHKNNISSAYDLAIMTQYSLLHYPKIWEILGKSEKNIIGYDSLGREMVHY
                     ARNVSWELLLRDNILGGKTGYTEDAGETMIVVMKAPGIVKGNIVMVILGVGIGERIPK
                     SIQLYNWVYNAYKWE"
BASE COUNT         2858 a         1545 c         1060 g         2864 t
ORIGIN      
        1 aagttaaaaa acacaacatt taaaaaataa cggagaacag ttctccgtta ttttttttaa
       61 atctgccgag aaacgatctc ggcaggtttt gtgtttatat tattattttt tgtaattatt
      121 cagtaggaat attagaaaac tttgtttgta atctttgttg catttgagga catgaaagat
      181 ttaatccgca aatatcaata acatcttgcc aagcatcagc agaagttatt acagaattaa
      241 cataagatgg attactaact tttataccgt ttacaaaacc ttctgcacat tttttacaag
      301 gattgtaagc gtctacttta ttttcctctg ctccgccact ggcatttaat tttcttgcca
      361 ttgccattac cgcatcgttt aaattccaag gattccctcc tccgccatat gctaaccaag
      421 tgcaaggtaa aaactgagaa ggacctattg cgcaagcaga ggacattgga acttcagttg
      481 gcttgtaacc aaacttttgg caaatagatt caagggctgc agcctcagta gaataagcac
      541 aatatggaga agaagaccag ttacaataat cttggtaacg cccaggcgtt cccaatccgg
      601 taccgaatct tgattcaatc tgtaaaattc ccaaaagcaa tgctggtctt acgccagtag
      661 ctgaagacgc tctttgaacg gctgaaaaaa ttgttgacca attaacctgg ccgacaagaa
      721 cccactgagc caaataacgc tcgatttgag acatcgctgt tcgtaattcc attagttctg
      781 cctctattct tgccatttct ttctcaagtg gagtttttgc tacggctaat ttttcaatta
      841 aatattgctg ttgttcttgt cttaacgcta aactttgttt tgacatttct cgcattgctt
      901 tatattgctc ttgttctttt ttcttttgag tcaattcttc tttttctttt tccatttgct
      961 ctttagcttt tcttatctcc tccattgatg atttcaaact tttttgcaat ctatctattt
     1021 cctctaattt ttgattaaaa tcagacaagg ttctatatcc taggagcatc tctatgtaag
     1081 acaatgtatt atactcgtaa atctgacgaa ttgcttcaga taaaattgct ttatgctgtc
     1141 caagggcatc gtctaattcc tttatcgctt tctctctttc ttgaatagca ttctctaatt
     1201 ccgctaatga taaatttaaa gaagcaattt cagtatttaa ttttttaata ttaaaagtaa
     1261 gattatttag agttttttgg gcagtagttt gttggctgtt aagactatta agttgttttt
     1321 gggtttgatt tttttgagct tcaagttttg cattacaatc tttaactttt ttctgaaaat
     1381 aattaagaat ctcgtctccg caatctttta atcttgaatc ctcagataaa ctctttaaat
     1441 catcatcgca ggtagcattt tttacttgat tacaaaaatc gctatcttcg gcaaaaacac
     1501 ccactggcaa aagcgggaga aaaagagagg tcaccaaagt aaaaattaaa gttaaaacaa
     1561 taattttttt ggaaaaagaa tgaaacataa ttttaaagca aaatgcaaaa tgcaaaaagc
     1621 aaaatgtaat gtaaaatgta aaaagaatta tttcttacct ttaagggtta aaatgcttga
     1681 ggcaattact tttgatattt gattaacttc tagtaataaa ttatttactt tgtcttttaa
     1741 ctctttgcga ctttctctta ttaagattaa ccagtatttt gtttcgtttg cagattttaa
     1801 agcaatctca aagaatttaa tataatcttt cttagaactg gatgactttg cttcaataac
     1861 atttgcacct atagaagttg cacttcttat caactgatcc actattggcc agaataatct
     1921 atcttttctt aatttatcag aaaattgtaa tatttctaaa gagaacttta ctaatctttt
     1981 attaaaatcc tctttgaatt ttgatttgtc atttgctgag tttttcactt tagtgaaaaa
     2041 tgaagtgtga aatgaaacta tcgtttcatt gaactgcatt ttgatttttg ctttttgcat
     2101 tttagtcatt accgtgcact ttcacacttt ctattatacc aattcctata aaaagtgaaa
     2161 gtaaactgga accaccgtaa cttaaaaacg gcaagggcaa tccactaatt ggtaaaagtc
     2221 caatattcat tccaatatta ataaatgctt ctgcacctac caaaataata aatccagaaa
     2281 ctaataatcg agaaaaatta ttattagatt tttttgcgat ttcaatacct cgccaaaaaa
     2341 acaaaccaaa cagtaaaaat aaaactaaca cacctatcaa tccaaacatt tcagtaaaag
     2401 cagcaaaaat aaaatcatta tgggcttctg gtaaaaaccc taattttgct tgagtgtaag
     2461 gttctgaaat tcccttgcca aaaagaccac ctgaaccaac ggcaataatt gattgctgaa
     2521 tatgataacc tgtgccttgg ggatctatat actctccttc tggattaata tatggttcaa
     2581 gaaaggttgt aaatctgtct ttttgttcct gagtaaaaac aaaattccaa gcaaccaaaa
     2641 agaataatac tcctacccca aaaataatca aaagatgttt tcgtttaata cctgatacta
     2701 acatcattga aaaccatatt aaaatcaaaa taatggctga tccaaagtca ggctgtaaga
     2761 atactaaagc tgttggtata aaagcatata caaaagaaag tacaatatgt cttttttggt
     2821 acatttccac gtgcctttgt gaaaaatatt ttgcaaagaa caaaataaaa ataatcttaa
     2881 caatctctac tggttgaaaa gtaaaagatc caaaatgata ccaagatctt gaacctctaa
     2941 tttctttccc taaaaaaaat actcctaata ataatataat agaaaaaaaa tatattgcca
     3001 aaataaaaag agaactgtcc cttagggctt taaaatcaat aaaagaaagg gaaatcgcca
     3061 atatgattcc aataagaaaa aaaattgctt gttgattaaa attagaaaaa tcgcctgaaa
     3121 acaaagacaa cccgtaaaga caaacaagtc ctaatattgt tagcaaaaca gcaaagatta
     3181 aaaataacca gtcaaatttt tttaaaaaaa tattacttga aatagccatt tttttagtcg
     3241 ttttcttttt cctcccattt tattttaaca attttaaaag aaacattttt tccttgctca
     3301 tcaatgctta tttctttttc tatttttttc tgctcatttc cttttacttc agttgtctcc
     3361 cctttttgaa aaataacttt gccattctcg tcttttattt caatcttaaa agcaacttct
     3421 tttgcaataa atttatcatt tggattttca atattaacag aaaccttata ggtaaagggc
     3481 ggatctgatg gttgatcatc tgattgaaat tcagcaaaaa catcttttat ttcaagtccc
     3541 ttaatttctt ctccctcaat agaagtaggt tctgctacaa aaaaccacac caaagccgct
     3601 atgcccagac aaaatgttaa aaaaccaaga gcaatattaa tttgttttat ctttcgacca
     3661 aaaggtgtta acataaatat ataaattcca atctactaat aatatttgaa cgatccgaat
     3721 attatttaaa tgtatgtttt tttggacaga atgattttga aggaaaaatc aaactattca
     3781 gatattaatg cttgggtagt ttgagaagga ataattattt caaaattttt atcagtccaa
     3841 ctgtctttac cagttaattt aaaattatta tgaatgacat caggcatatt agatggtaca
     3901 atctttaatt gcagaaaata ttctttttta aaatttgctg aaatattgcc aatatttaaa
     3961 actaattctc ttttattttc attaaactga atatcagtat tacttggaaa aaatttattt
     4021 aaaaaagaaa cattggcggg caaggtacca gtaatcttca catctgttaa ttgattacca
     4081 taatttttta tttcaaaacc aattacaaaa ttattttctt tgccaataat tattgaagaa
     4141 atattaatat caaaaatact gcgaacatct tgaggcagaa attctgattt tgaataaatt
     4201 ttttctttaa aagacaactt tgaagatacc tttgtttgaa aagatttttc aatagttcca
     4261 attttaatta tttcttttat aacagcattc tctttataaa aagattcatc gtaatccttt
     4321 aaattaatag taaatccaac actaccactg ccatatggac ctagaaataa taaatcagga
     4381 aagttgtctg aagaaaaagt aattttttta ccttcaattt ttcctcctgg cgctcgcaaa
     4441 gtagaaaaat ccaaaacatc actttctaat tctacggtta atgaaaggcc ccgataaatg
     4501 tcttcacctg tattcataaa gtcaacaata aaagcaagat tttcaccggg agaagcaaca
     4561 taatttgttt ctccgtttac ctttattgaa gcatcgaggg tagaggtttt tatttttact
     4621 attttttcta tgctggcaag aggaataaac tcatataatt tttcatcaaa aataccaaat
     4681 tctatcttaa atagtttttc atctcctact tcaccattta ataatccctc aatttgtctt
     4741 ttacctccct cattttcatt aacacttccg atatcaaaaa taatttttcc tgactcttgt
     4801 ctttcttctt ctgttttatt tgaaattctc tcaaatcctt ctggcggtga aaatctaatt
     4861 tgaacatttt ttaaagggaa aggaaaacta gattgccaaa aaaaagaaat agaaactttt
     4921 tcacctggtt ctactttaag cggggcatcc attgaaaaaa ctatcgatgt atcagaaatt
     4981 cgcgttgaga atgaagtatc attttgaaaa aatgcagaaa gtccttccgg attataggtt
     5041 aaagatgcct tggcggtttt tgtttcttct tttacaccaa aaattaatcc tgaaaatgtc
     5101 tcagtttttt cttcgcctcc ggctatcttt ttaataattc ttttttcttt tttctttact
     5161 gaaccttcat tatcaaacac tccttcaggg tattcaaaaa ttaattcgag attctcaatg
     5221 gtaatctcgc tttggttttt tatactaact acccaactaa tttcttttcc agctactata
     5281 tcctgggggc cagaaatttt caaaataata ttttccttgg aaaaagtatt cttaaaaaag
     5341 aaaaaataga caaatgttcc tattaaggcc actaaaagaa caaaaactaa aattattgtt
     5401 ttcttttttg tccaatgaag attcatatga taaatattag attaatttta cgctcggcaa
     5461 ttaaaattat tatatcacaa aattttcctc ctccaaaatc cacgaaagat aatatttaga
     5521 aagatccttt aactctcttt cctgaacagg atttatcttt aatcttttta gagtattttt
     5581 atcttttaaa attattaacc ttaaaatttt aatcgcatca cgagaaattt tttgacattt
     5641 agcagacgat aaataattcg tttttatctc tcgccccgcc tcgctttcgt tcaaatcggc
     5701 atttaatgaa cgatgagaac ctgccttatc ttccgctttt cttcgtttcg cccgactcga
     5761 catttgacat ttattgcata ctattccccc ttcttttgct gaaaaataaa attccccccc
     5821 agacagttta ttttggcata taatacaatg atataactct ggctcaaaac ccaaaaaaga
     5881 cagcaaattc cactcaaaat agcgaacagc cattgataat tgataattaa cagatggcaa
     5941 tatatcaatt ctttcaagtg tttcaaaaag taaatcccaa atctcttcat ctttttcctc
     6001 gccagttatt aatctatcag taatgtcaca cacataaaga ccagcacggt attttctaat
     6061 ctctcttttt aaagaccaaa aaccattttt taaaaaaaca tctgttgcaa taaaaaaatt
     6121 tttaccgcga ataaattcca aagaaacatt gtttaatccc tgaagtccaa attttaattt
     6181 agcttttatt ttcctaattc cctttcccat tacctcaaac tttccaaagt ttttggtata
     6241 aagagtaaat attttatccg tctctcctaa ttccttttct ttcaaaacaa ttgcattagt
     6301 tttcattgtc tgatactcca taaaataaat ttatcctaac aaccttattg caatgccgat
     6361 aattgccaac acaaccccaa taatccaaaa cctcattacg atttttgctt cagaccatcc
     6421 tttggcttga aaatgatgat ggataggcgt agcaagaaat attttctttt taaaaaattt
     6481 ctttgatatc aattgtaaaa tcactgaacc tgattccatt accaaaagtc ctccaataat
     6541 gggtaaaacc aaaactgaat ctgttaaaaa agctaccact gcaagaactg ttgtcagggc
     6601 caaacttcct gtctccccca tataaaatct agctggcgga acattaaacc atagaaaaga
     6661 aagaactgtc cccgtaatca caccacaaaa agccgctaaa tcaatttgtt cttgagcaag
     6721 agcaataact ccaaacgcag caaacattga agcaaacgct cctcctgtca aaccatctaa
     6781 tccatcaatt acacctcctg accagcaagc caatgtaaca ataataaaaa gtaaaatata
     6841 aaatggccct ataaatgcat caccgttgct cggaatatga atagtttgcc aatcaagttt
     6901 aaaataaaac cacaatccgc caacaagacc aattagcaac accaccaaga acctttgcca
     6961 aaatgtaaga ccaccagcga tatactttcc cctacttctt acttgtaaaa tatcgtcaac
     7021 caatcctaac aaagctgcag caactaaagc aaacattggc agccaggttt gagaccgaga
     7081 aaaaaaatta agcttttgaa gccaaaaatt atctgggaaa aactgagaaa gagaccaaaa
     7141 aattaaggca gtaactaaaa cgctgcccca gattaaaaca ccaccaaacc gaggaactga
     7201 aacctctttt ttcttatgaa atttattaaa aacttctgct tctttaccat caatagtttt
     7261 tgttcgacta cttttccgcc ataatttatt acgatataaa aaatttgtaa gagcaggtgt
     7321 tgctaaaatt gctaagacac tagcaaaaat tgttaatgaa aaaatcttaa taatattaac
     7381 agcaagagga gacataatat aaaattccaa attccaaatt tttaaattct attatttgaa
     7441 aattaaaaat cttattccca tttataggca ttataaaccc agttataaag ttgaatggac
     7501 ttaggaattc gttcaccaat tcccacaccc aaaataacca ttacaatatt tccttttaca
     7561 attcctggcg ctttcattac tacaatcatt gtctcgccag catcttcggt ataacccgtt
     7621 tttcccccta aaatattatc tcttaaaagt aactcccatg aaacattacg agcgtaatgt
     7681 accatttctc ttcccaacga atcgtaacca ataatatttt tctctgattt acctaagatt
     7741 tcccatattt taggataatg caaaagagaa tattgggtca ttatagcaag atcataagcc
     7801 gatgagatat tatttttatg atcgtcctca tctaatccat gagggtttac aaaaaaagtg
     7861 tttaataatc caatttcttt tgccttttcg ttcattgatt ttacaaacct ttcttcaccc
     7921 attatttcgg ctaaagcgct tgtcgcatca ttgctagata ctaaaagagc tagatttaat
     7981 aaattttcca ctgtcatttt ttctcccgag cacaagctag attttcctaa tgttttaata
     8041 gctttctcgc ttatttccac ctctttgtcc aaaggataat tatcaatcac taccattgca
     8101 gtcattattt tggttaaact ggcaatgggt aattgttctt caatattttt ttcatataaa
     8161 atattttctt ttccataagg cgaaaataaa actaccagac ccgctttttc tcctaactga
     8221 ggtgtccaaa aatcttcttc ttttattgga aaaaaacttg aaaaagaaat attatttttt
     8281 tcttcttctg ttaacagttt tgatacttga gcagcattaa tcgtttg
//
LOCUS       DAOTQD010000025         7840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_8220_length_7840_cov_7.568401, whole genome shotgun sequence.
ACCESSION   DAOTQD010000025 DAOTQD010000000
VERSION     DAOTQD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_7840_cov_7.568401"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..403
                     /gene="trxA"
                     /locus_tag="P5570_01685"
     CDS             74..403
                     /gene="trxA"
                     /locus_tag="P5570_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRR39449.1"
                     /translation="MFVFNFTDQNFEKEVFKSQKPVLVDFWAEWCMPCKMMSPIIEEI
                     ADEFNDRIKVGKLNIDENPFIATTFSIDAIPTLILFKNGKVVQRIKALQPKEVIEEMI
                     HSVIKEN"
     gene            complement(515..585)
                     /locus_tag="P5570_01690"
     tRNA            complement(515..585)
                     /locus_tag="P5570_01690"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Gly,seq:tcc)
     gene            complement(644..1018)
                     /locus_tag="P5570_01695"
     CDS             complement(644..1018)
                     /locus_tag="P5570_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR39450.1"
                     /translation="MDQQKRVLLVEDDIFIAEIYALSLQKAGFKVIVAEDGEKGVQMA
                     KENHPDLILLDLLLPKLSGLEVLKILKNDPELKDTPVVIITNFSDSESTKKSFEMGAL
                     DYLVKVNIDSNDIVQRAKEILL"
     gene            complement(1036..1106)
                     /locus_tag="P5570_01700"
     tRNA            complement(1036..1106)
                     /locus_tag="P5570_01700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1072..1074),aa:Pro,seq:tgg)
     gene            1263..2759
                     /locus_tag="P5570_01705"
     CDS             1263..2759
                     /locus_tag="P5570_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRR39451.1"
                     /translation="MSSSRAFFYFCLCFLGGVLIASFSNPLSISIGIFVLFFALIIGV
                     AVFIVFYKKDIAIIGLCIFTLIFGIYWEEKFENEIIPSLKDIHFYNEKGNITFEGIIL
                     EKPEERMKNTRIIVESQEIIDDNDSKTSVRGKVLITLPVGVDFDYGDKIKVTGKLQTP
                     QKFTEDFDWPEYLRKERIYSTMYEPEIKKISSGNGNPVLAKIFSLKSKLKNTAELILP
                     PEGALLSAMTLGDKTRISEKLSNDLSRAGLSHMVAISGLHIMVLFSILLSCFLWIGCW
                     RKEATIFTILFLIFYILMIGAPSSAIRAGVMGGLLYLGMAFGRLNQSSRAITFAATGM
                     VISNPLILTRDVGFQLSFLASLGLIYLLPVLKEKWQVKDSMIKELLCITLAAQAFCLP
                     ILIYNFGQVPILSPLSNILVVPLLSWLLGASLLFLLLGTVFPPLALPLSFIIWLPYTF
                     IVRILDLISAVPFSTINFKVSLVVVFLCYILLFYFTVRENKKVKISNF"
     gene            3188..4105
                     /locus_tag="P5570_01710"
     CDS             3188..4105
                     /locus_tag="P5570_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR39452.1"
                     /translation="MSKKKIQFAVLVFLFLGVVLVFKAVFAFNHSSLIEVNFFDVGEG
                     DSIMIKLPGNIQVLIDGGPSGSVVEQIAKEMPFFDREIELIILTHPDNDHIAGLFEVF
                     KSFKVDKILMSDIEGKIKEKDLYLSFQEIVQEEGSEVIFAQQGKKISFLPVAGSSAEE
                     VITPQIFILWPLQDLKTEEINDFSVVAKLNFGEMDFLFTGDISNKIEYQLLANNLTLD
                     QIGDVNLQSEVIKIAHHGSKYSSNEYFLRAVSPEVAVISVGENHYGHPAPEVLDNLKK
                     YGIIIYRTDESGNIKIISNGKNYQILTNQ"
     gene            4161..5282
                     /locus_tag="P5570_01715"
     CDS             4161..5282
                     /locus_tag="P5570_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRR39453.1"
                     /translation="MEKNLHSPEEKKSLSEMKDFEFPIFKTKTEGDSHSFNLTDPQER
                     KDYFYLKAGDEIKKLQDYLKKHSFIAYLLGKKNSGKGTYSQMFREIVGEDLAVHISVG
                     DIVREVEEEIKDEGKKQELLEYLKKNYRGYIPLEETFEALSSRSTKTLLPTEFILALT
                     KREIDKHPKKTLFIDGFPRNLDQISYSLFFRDLAGYRYDPDIFILVDVPEAVIEKRIQ
                     YRVVCPVCQTPRNLKLLPTNKVGYDKEKKEFYLICDNPGCQNARMIQKEGDAQGIEPI
                     RERLKLDEQLMRKAFSLYGVSKIFLRNAVPVEVADKYVNDYEITPEFDYRWDPEKEKV
                     EIIKNPWVVLDNEGRPSYSLMPEAVVVSMIKQLVNVLGL"
     gene            5290..6309
                     /gene="recA"
                     /locus_tag="P5570_01720"
     CDS             5290..6309
                     /gene="recA"
                     /locus_tag="P5570_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRR39454.1"
                     /translation="MAKKKNQSDTNLQDTVDSIKEKFGEGAIMKLGEVRAVDVEAIPT
                     SSLSLDIALGIGGIPKGRIIEIFGPESSGKSTLALHIVAEDQKKGGIAAFVDAEHSLD
                     PEYARRIGVNVDDLLISQPDTGEQALDIVETLVRSGKISVIVVDSVAALTPRAEIEGE
                     MGDQQIGLQARLMSQALRKLTAISAKAGTSIIFLNQTRMKISTFGFGNPETTPGGKAL
                     KFYASVRIELRPIAKIKKGDEVMGNRVRAKIVKNKVAPPFRTAEFDIMWNEGISKMGD
                     LLNTGLKYGIVEKKGNTLEFKGTKLGVGTESSKKFLNENPKIAKDIKKEIIEKWQEEK
                     KTPQS"
     gene            6276..6785
                     /locus_tag="P5570_01725"
     CDS             6276..6785
                     /locus_tag="P5570_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007402889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRR39455.1"
                     /translation="MAGREKNTAVVKERSAGAIIFRKEIFLEKKDKIYYLLLHYPSGH
                     WDFVKGHIEKGESERETAKREIKEETGIKDVKFIPGFIEKINYFFYRNLNDQKDPPLS
                     FNQNNKVLVLKEVVFYLAETKEKNVEISFEHIGYEWLEYKEAIKKVTFKNAREVLKKA
                     NEFLKSYLS"
     gene            6800..7705
                     /locus_tag="P5570_01730"
     CDS             6800..7705
                     /locus_tag="P5570_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HRR39456.1"
                     /translation="MTKAVLLFSGGLDSMIASQILLKQKIKVIPVCFFSYFFDCQRAK
                     KSAESLGLKLRVIDFSEEHLKIIKSPKYGRGKGLNPCIDCHLLMAKEAKKLLKKTNTD
                     FVATGDVLGQRPFSQNLKSLKIIERESGLTGLILRPLSAQLLPETLPEKRGWVNRETL
                     FGIKGKSRSPQLLLAKKFNIKEFPTPSGGCILTDIEYSMKLKELFEKVPDCDGNDCQL
                     LRKGRVFWRKDDSNDDILIVVARNEKECLELKKMKKETDIILEPKNFAGPVVLARGFL
                     KKKKKNNKKIIQDAKELLLRYSKVR"
BASE COUNT         2659 a         1107 c         1512 g         2562 t
ORIGIN      
        1 tttttaggag ggggcttgaa atttgcaact tttttagtta tatttttaat gtaaaatatt
       61 taagaatctt tttatgtttg tttttaattt tacagatcaa aattttgaaa aagaagtatt
      121 taagagtcaa aagccggttt tagttgattt ttgggcagaa tggtgtatgc cctgtaaaat
      181 gatgtctcct attatagagg agattgctga tgaatttaat gataggatta aagtaggaaa
      241 actcaatatt gatgaaaatc catttattgc tactactttt tcaattgatg ctattccaac
      301 attgatttta tttaaaaacg ggaaagtggt tcaacggata aaagctcttc agcccaaaga
      361 ggtgattgaa gaaatgatac attcagtaat aaaagaaaat taaataaaaa attaaaaagc
      421 ccaatttaat tgggcttttt aatttgtaaa aactcagcgg tcaaagaaaa atgcgttttc
      481 ttttcccact aaatttttta aaaataaaaa atttagcggg tgatggggat cgaacccacg
      541 tacacggctt ggaaggccgt tgcactacca ctgtgctaca cccgcgcaaa gcaatcaaat
      601 aaaaagtatc cactcgaggc agctctttac cccatttttt attttagagt aatatttcct
      661 ttgctctttg gacaatatca tttgagtcga tgtttacttt taccagataa tcaagtgctc
      721 ccatctcaaa agattttttt gtgctttcac tgtctgaaaa attagtaata atgacaacag
      781 gtgtatcttt taactcagga tcatttttta atatttttaa tacttcaaga ccactcaatt
      841 ttggcaaaag caaatccagt agaattaaat cagggtgatt ttctttggcc atttgaacac
      901 ctttttctcc gtcctcagcg actattactt taaaccctgc tttttgaagg gaaagggcat
      961 agatctcagc aataaagatg tcgtcctcta ctaatagaac ccttttttgt tggtccatat
     1021 ttttgtctgt ttttatcggg gcatccagac tcgaactgga actaaatcct cccaaaggac
     1081 tcgtgctacc attacaccat gccccggatt tatagggcta gtaattaaat gcttattttt
     1141 tattagtata tactaataag agacaaagag caagacagca ggcatttttt gatttttgca
     1201 atttttttat ttttggttta aatttattta aggttttgtt ttatttatta taaaaaattt
     1261 ctatgtcttc ttcaagagca tttttttatt tttgtctttg ctttttagga ggcgtgttga
     1321 tcgcttcttt ttccaatcct ttatcaataa gtattggtat ttttgtttta ttttttgcgt
     1381 tgattatagg ggttgcagtt tttattgttt tttataaaaa agatatagca ataattggtc
     1441 tttgcatttt tactttaatc tttgggattt actgggaaga aaaatttgaa aatgaaatta
     1501 taccatcttt aaaagacatt catttttata atgaaaaggg aaatattact tttgagggaa
     1561 ttattttaga aaagccagaa gaaagaatga aaaacactcg cattatagtt gaaagccagg
     1621 aaataattga tgataatgat tctaaaacat cagtacgggg taaggtttta attactttgc
     1681 cagttggtgt tgattttgat tatggagaca agattaaagt aactggcaag cttcaaactc
     1741 cccaaaaatt tactgaggat tttgactggc cagaatattt aagaaaagaa agaatttatt
     1801 caacaatgta tgaaccagaa ataaaaaaaa tttcttctgg caatggcaat ccagtattag
     1861 caaagatttt ttctctaaaa tcaaaattaa aaaatactgc cgaacttatt ttgccaccag
     1921 agggcgctct cttgtcagca atgactttag gcgacaaaac aagaatttct gaaaaattaa
     1981 gtaatgatct ttcaagggca ggtctttctc atatggtagc aatttcaggg ctacatatta
     2041 tggttttatt tagcattttg ctttcctgtt ttttatggat cggttgctgg cgcaaagagg
     2101 caacaatttt tactatttta tttttaattt tttatatttt aatgattggt gctccatcat
     2161 ctgcaattag agcgggagtg atgggaggat tattatatct aggaatggct tttggtaggt
     2221 taaatcaatc atcaagagca attacctttg cagcaaccgg aatggtaatt tcaaatcctt
     2281 taattttaac tcgcgatgtt ggttttcagc tctctttttt agcctctctt ggacttattt
     2341 atcttttacc cgttttaaaa gaaaagtggc aggtcaagga ttcaatgatc aaagaattgc
     2401 tttgtataac ccttgctgct caggcatttt gcttgccgat tctaatttac aattttggtc
     2461 aggtgcctat tttgtctcca ctttcaaata ttttggttgt acctcttcta tcatggcttt
     2521 tgggagccag tcttttattt ttactcttgg ggacagtttt tccacccttg gcactgcctc
     2581 tttcttttat tatttggttg ccttatactt ttattgtaag aattttggat ttgatttcag
     2641 cggttccatt ttctacaatt aattttaaag tttctctcgt tgtggttttt ctttgctaca
     2701 tattattatt ttattttaca gtaagggaaa acaaaaaggt taaaatttct aatttctaat
     2761 ttctaatttt caataacgcg agatcgctga aagcgatcga agcgtcgaaa agaagataaa
     2821 atcttctttg aacgctcctc gcgatgatag tagacgtccc cccttcttgt ctttgcgagg
     2881 agtgaacgaa gtgaacgacg aagcaatccc gtggtaatac tctgggattg ccacgtccta
     2941 ctgcggtagg actcgcaatg acagtgaaga ggggatcgcc acgctcttcg ggctctcaaa
     3001 gaaaatttca ttttcttttc gacgagctcg cttcgctctc tcgttcgcga tgacagtggg
     3061 gaggggtcgt gatgacaaga aggtattgtt gcataaattt ttgaacctaa cttaggttta
     3121 gaatttagaa cattaagatt tcattgatca ttgaacattg gagtttggga atttttattt
     3181 tcattttatg tcaaagaaaa aaattcaatt tgctgttctt gtttttttat ttttaggagt
     3241 ggtcttggtt tttaaagctg tttttgcttt taatcattca tcgcttattg aggttaattt
     3301 ttttgatgtg ggcgagggtg attcaataat gattaagctt cctgggaaca ttcaggtttt
     3361 aattgatggt ggtcccagtg gttctgtggt tgaacagatt gcaaaagaga tgccgttttt
     3421 tgatagggag attgagctta taattttaac ccatcctgac aatgatcata tcgctggtct
     3481 ttttgaagtt tttaaatctt ttaaggtgga taagatttta atgtcggaca tagaaggcaa
     3541 aataaaagaa aaagatctct atcttagttt tcaagaaata gttcaagaag aaggaagcga
     3601 agtaattttt gcacaacaag gaaaaaagat ttctttttta ccagttgcag gatcatcagc
     3661 agaagaagtt atcaccccgc aaatatttat tttatggcca ttgcaagatt taaaaacaga
     3721 ggaaatcaat gacttttcgg tcgtagcaaa attaaatttt ggagagatgg attttctttt
     3781 tactggagat atttcaaata aaatagaata tcaactgtta gcaaataatc tcaccctaga
     3841 ccaaatagga gatgttaatc ttcaaagcga agttatcaaa atagcgcatc acggcagtaa
     3901 atattcaagt aatgaatatt ttttaagagc agtttcgcct gaggttgctg tgatctcagt
     3961 aggtgaaaat cattatggtc atccagcacc agaagttttg gataatttaa aaaaatatgg
     4021 tataattata tataggaccg atgaaagcgg aaatataaag attataagca atggtaaaaa
     4081 ttatcaaatt ttaactaacc agtaataaaa tttaattttt ttaaaattct gttaaatttt
     4141 atatctggat cttttaacct atggaaaaaa atttacactc accagaagaa aaaaaatcat
     4201 tatcagagat gaaagatttt gagttcccaa tttttaaaac aaaaacagag ggagattcgc
     4261 attcttttaa tttaactgat cctcaagaaa gaaaggatta tttttattta aaggcaggag
     4321 atgagatcaa aaaattgcaa gattatctta aaaaacatag ttttattgct tatctgttgg
     4381 ggaaaaagaa ctctggaaag ggtacttatt ctcaaatgtt tagagaaatc gtaggtgagg
     4441 acctggctgt gcatatttca gtgggtgata tagtaagaga ggttgaagaa gaaataaaag
     4501 acgagggaaa aaaacaagaa ctgttagaat atcttaaaaa gaactatcgt ggatatattc
     4561 cgttagaaga gacctttgag gcgttgtctt cacgcagcac aaaaactctt ttgccaacag
     4621 aatttatttt ggctcttacc aaaagagaaa tagacaaaca tcccaagaaa actcttttta
     4681 ttgatggttt tccaagaaat cttgatcaga tctcttactc tctatttttt agagacttgg
     4741 cgggttatcg ttatgatcca gatattttta ttttggtaga tgtccctgag gcagttattg
     4801 aaaaaaggat tcagtatcgt gttgtttgtc cagtctgcca aacaccccgt aaccttaaac
     4861 ttttaccaac aaataaagta ggttatgata aagaaaaaaa agaattttat ttaatttgcg
     4921 ataaccctgg ctgtcagaac gctcgaatga tccaaaaaga aggagatgct cagggcattg
     4981 aacccatcag agaaagatta aaactagatg aacaattgat gagaaaagcc ttttctcttt
     5041 acggtgtgtc taaaatattt ttgagaaatg ctgttccagt tgaggtagct gataaatatg
     5101 ttaatgatta cgaaattaca cctgagtttg attatcggtg ggatcctgaa aaggaaaaag
     5161 tagaaataat taaaaatccc tgggttgttt tggataatga aggaaggcct tcttatagtt
     5221 tgatgccaga ggcagtagtg gtttcaatga tcaaacaatt ggttaatgtt cttggattgt
     5281 aataaaacta tggcaaagaa aaaaaatcaa tcagatacaa atttgcaaga tactgttgat
     5341 agtattaaag aaaaatttgg agaaggtgca attatgaaat tgggcgaggt aagggctgtt
     5401 gatgtagaag caatacctac ctcatctttg tcattggata ttgccttggg tattggtggc
     5461 attccaaagg gtagaataat tgaaattttt ggaccagaga gctcaggaaa atcaacattg
     5521 gctttgcata ttgtggctga agatcaaaaa aaaggcggga tagccgcttt tgttgacgcc
     5581 gaacactctc ttgatcctga gtatgcgcgg cgcattggtg tcaatgtaga tgatttatta
     5641 atctctcagc cagataccgg agaacaagca ttggatattg tagaaacctt ggtaagatct
     5701 ggaaaaatat cagtgattgt ggttgattcg gtggcggctt taacaccccg ggcagaaatt
     5761 gaaggtgaaa tgggtgatca gcaaataggg cttcaggccc gtttaatgtc acaggcttta
     5821 agaaaactta cagccatttc tgccaaagca ggcacctcaa ttatcttttt gaatcaaacc
     5881 agaatgaaga tcagcacttt tggttttggt aatcccgaaa caacaccggg tggcaaggca
     5941 ttaaaatttt atgcttcggt gagaattgaa ttaagaccga ttgctaaaat taaaaaagga
     6001 gatgaggtga tgggcaatcg cgttagagca aaaatcgtta aaaataaagt tgctcctcct
     6061 tttagaactg ctgagtttga tattatgtgg aacgagggaa tttcaaaaat gggtgatctt
     6121 ttaaatactg gcctcaaata tggaatagta gagaaaaaag ggaacacttt ggaatttaag
     6181 ggcacaaaat tgggcgttgg aacagaatca tcaaaaaaat ttttaaatga gaatcctaaa
     6241 atagcaaaag acattaaaaa agaaataatt gaaaaatggc aggaagagaa aaaaacaccg
     6301 cagtcgtaaa agaaagatct gccggcgcaa taatttttag aaaagagatt tttttagaga
     6361 aaaaagataa aatttattat cttcttttgc actatccttc ggggcactgg gattttgtca
     6421 agggacacat tgaaaaagga gagagcgaaa gggaaacagc taaaagagaa ataaaagaag
     6481 aaactgggat taaagacgtt aaatttattc ctggatttat agaaaaaata aattattttt
     6541 tctacagaaa tctaaacgat caaaaagatc cacctttatc ttttaatcaa aacaacaagg
     6601 ttttagtttt aaaagaggtg gttttttatt tagcagaaac aaaagaaaaa aatgttgaaa
     6661 tatcgtttga gcatatagga tatgagtggt tagagtataa agaggcaata aagaaagtga
     6721 cttttaaaaa cgctagagag gttttaaaaa aagcaaatga gtttttaaaa tcctatttgt
     6781 cttaaatttt taaacgagta tgacaaaagc agttttactg ttttcaggtg ggttggattc
     6841 aatgatagca tcacaaattt tattaaaaca aaaaataaaa gtgatccctg tttgtttttt
     6901 tagctatttt tttgattgcc agagagcaaa aaaatcagca gaatcattag gattaaaatt
     6961 aagagtaatt gatttttcag aagagcattt aaaaattata aaatccccaa aatatgggag
     7021 gggtaaagga ttaaatcctt gcattgattg tcatttattg atggcaaaag aagcaaaaaa
     7081 attattaaaa aaaacaaata cagattttgt tgcaacaggc gatgtattag gacaaagacc
     7141 attttcacag aatttaaaat ctctaaaaat aatagaaaga gaatcagggt taacaggatt
     7201 aatattaaga ccattatcgg cccagttgtt gccagagacc cttccagaaa aaagaggatg
     7261 ggttaataga gagacattat ttggtataaa aggaaaatca agatctcctc aactattatt
     7321 agctaaaaag tttaatataa aagaatttcc aacacccagc ggcggttgta ttttaacaga
     7381 tattgaatat tcaatgaaat taaaagaact tttcgaaaag gttcctgatt gtgatggcaa
     7441 tgattgtcaa ttgttaagaa aaggaagggt gttttggaga aaagatgatt ctaatgatga
     7501 tattcttatt gtggtggcaa gaaatgaaaa agaatgtttg gaattaaaga agatgaaaaa
     7561 agaaacagat attattttgg aaccgaaaaa ttttgctggg cccgttgttt tggcaagagg
     7621 gtttttgaaa aaaaagaaga agaataataa gaagataatt caagatgcaa aagaattact
     7681 tttacgatat tcaaaagtta ggtagattca agtttttaac gcaatcatac aacgggattg
     7741 ccacgtccta ctgcggtagg actcgcaatg acaagagaaa aggatgtcat tgcgaggagt
     7801 gttcaaagaa gattttatct tcttttcaac gcttcgcccc
//
LOCUS       DAOTQD010000026         6816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_9677_length_6816_cov_7.959917, whole genome shotgun sequence.
ACCESSION   DAOTQD010000026 DAOTQD010000000
VERSION     DAOTQD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9677_length_6816_cov_7.959917"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..652
                     /locus_tag="P5570_01735"
     CDS             260..652
                     /locus_tag="P5570_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRR39457.1"
                     /translation="MRKKQKQKGGFWRIFSSKVFLFLIIFIFAGMAKATFDKYVSWRQ
                     ANSALTEVEEKIQSEQQKSKDLKKEIDEIRSEEYIERVTKERLNLVKPGEKVIYVLPE
                     TPAEQEESNEEIKTNEGLWEKIKGIFVK"
     gene            658..728
                     /locus_tag="P5570_01740"
     tRNA            658..728
                     /locus_tag="P5570_01740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:690..692,aa:Gly,seq:ccc)
     gene            803..1213
                     /gene="rpsL"
                     /locus_tag="P5570_01745"
     CDS             803..1213
                     /gene="rpsL"
                     /locus_tag="P5570_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRR39458.1"
                     /translation="MPTLHQLIKKPRKDKKTKTKVPALSFGFNPIKNRPTYYPSPFKR
                     GVCIKVFTTTPKKPNSALRKVARVRLQNGMEVTAYIPGEGHNLQEHSIVLIRGGRVKD
                     LPGVRYHIVRGVLDTEGVKNRKKKRSKYGAKKGK"
     gene            1259..>1687
                     /gene="rpsG"
                     /locus_tag="P5570_01750"
     CDS             1259..>1687
                     /gene="rpsG"
                     /locus_tag="P5570_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRR39459.1"
                     /translation="MLQPDPKYNNLIIQKFINQTMRQGKKTLAQSIVYNAFDIIQEKT
                     KKNPLEVFSQALKNAAPHMETKPRRVGGAIYQVPIEVKGDRQRTLAMRWILDAARSKK
                     GKSMDEKLAEELILASKNEGAAIRKKINVERMAEANKAFAH"
     assembly_gap    1688..1787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1788..3905
                     /gene="fusA"
                     /locus_tag="P5570_01755"
     CDS             <1788..3905
                     /gene="fusA"
                     /locus_tag="P5570_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRR39460.1"
                     /translation="YPIERYRNIGFIAHIDAGKTTVSERVLFYTGVSHKIGEVHEGQA
                     IMDWMVEERERGITITSAATTCFWLPTYLKKEEGEMEPEERKSKEYQINVIDTPGHID
                     FTVEVQRSLRILDGAVVIFDGVSGVEPQSETVWRQADKFHVPRLCFVNKIDRTGADFK
                     KDVESIKTKLTPNVVILQMPLGQEENFQGVIDLLSLKAYLFEGEHGENIKEVEIPLEL
                     KDEAISLHQQTIEKIVEQDEALTEKYLEGNIPDLNELKKVLRKSVIDFKLVPVLCGSA
                     LKNKGVQLLLDAIVDYLPSPIDLPPAKGENPKTGKEEIREPKDNAPFSALVFKVASDP
                     YVGELSYFRVYSGTLKKGSYVLNPTNNTKERIGRLLRMHSNERIEIDEVFAGGIAATL
                     GLKDTKTGDTLCDENAPILLEKITFPEPVISISVEPNTKEDQEKLALSLQKLAKEDPT
                     FKVKTDEETQQTIISGMGELHLEIILDRLRREFGVNINSGRPQVAYRETISLSSEAEG
                     KYIRQSGGRGQYGHVFLRVEPKKRGEGFEFVDEIKGGAIPKEFIPAVKKGVIEARDKG
                     IIAGFPLTDIKVTLYDGTFHEVDSSDFAFKIAGSIALSEAVKKAKPVLLEPIMKTEVR
                     CPEEFLGDVIGDLSSRRARIEETKSILNIKVIDAYVPLSEMFGYATVLRSLTQGRGTF
                     TMEFDHYQEVPQSVAQQIIEGKK"
     gene            3922..5211
                     /gene="nusA"
                     /locus_tag="P5570_01760"
     CDS             3922..5211
                     /gene="nusA"
                     /locus_tag="P5570_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRR39461.1"
                     /translation="MDLKSISYNILQLAEEKGVSEEKVIEIIEKALAAAYKKEYGNKS
                     QRYGAHFDKDSGKVEFFLIKKVVNEDMLLPANASVAQADSKKEGKEEKKSNKETTTDK
                     VIEEEGEEQKKFKFNPERHIMLNEAKKIKPDVKPGDQLEFPVPSLEKFGRIATQTAKQ
                     VLLQQIREAERESILQEFQKKEGEIVSGVIQRREGDTIFVDLGKTIGILLKEDQIPGE
                     FYKLGNRLRFFLLKVKDSSHGPEIYLSRAHPKFLSKLFELEVPEIASGSVEVKAVARQ
                     AGSRSKIAVHSKEEGIDPVGSCVGARGSRVNAVMQELGREKIDIIAFSEKPEEFIENA
                     LSPAKVVNVTINENKAVVEVPKDQLSLAIGRDGQNVKLASDLTGWKIDVVPANIDESD
                     KKEANKEKKASVKEPKEEEKKTEEKTEKSNDNKKSQK"
     gene            5269..5745
                     /locus_tag="P5570_01765"
     CDS             5269..5745
                     /locus_tag="P5570_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004578240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRR39462.1"
                     /translation="MSIKVFVEIPKGSSQKYEFDEEEKIIKLDRTLYSPMYFPFEYGF
                     IKGTMGGDGDPLDCILLASSPTFPGCLVEAEPIGLLEMEDEGGTDTKIIAVPTTKIDP
                     RFEGINDVNDISEHTKKEIKEFFENYKNLEPGKWVELKGFRPKHEAEKLIEKSSNL"
     gene            5838..6743
                     /gene="mutM"
                     /locus_tag="P5570_01770"
     CDS             5838..6743
                     /gene="mutM"
                     /locus_tag="P5570_01770"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRR39463.1"
                     /translation="MPELPEVETIVRELRKKVLKRTFFDMWTDNPKMIYFCNDFKKQV
                     KDLRDINEFKKEIKGKVINNIWRRGKNIIFDLSKNKSLLIHQKLTGHLLVGQWTLNKG
                     LWEPIDNNFLKEPVNRYIHLMFWLSGDLMLALSDLRKFAKVELWDSDKLKKSRELNEL
                     GPEPLDKNFTFQIFKNVLPKKGKIKQVLMNQTVVAGIGNIYSDEILWEARVYPLKDVS
                     KLSEKELNEIYQAIKKILKTAIKLKGESFSDFRRTSGEKGNFDRERKVYQKEGEKCFR
                     CGATIQRVKLGGRSAHFCPVCQKSD"
BASE COUNT         2422 a          942 c         1415 g         1937 t
ORIGIN      
        1 ggtttaaatc agaaaaagac aaaggtacaa ctttttattt tactctgcca atcagccatc
       61 aataataaaa acaattccaa tcacaaatta attaaaatgt tttagatttc tattgatatt
      121 ttatttaaat caatattatt ttttttgtaa aattacaatc aaactgaggt tatatattct
      181 ttttattgct cataatattt attaatattt gacttttttt tgaaaaaaga atatatttta
      241 taacagtaga taagatttaa tgagaaaaaa gcaaaaacaa aaaggtggtt tttggcggat
      301 tttttcttcc aaagtttttt tatttttgat aatttttatt tttgcgggca tggcaaaagc
      361 tacttttgat aagtatgttt cctggagaca ggcaaatagt gccttaaccg aggttgaaga
      421 aaaaattcaa agcgagcagc aaaaaagtaa ggatttaaag aaagagattg atgagattcg
      481 aagcgaagag tatattgaaa gagtgacaaa ggaaagatta aatttagtaa agccaggaga
      541 gaaagtgatt tatgtgttgc ctgagacgcc agcagaacaa gaggaatcaa atgaagaaat
      601 taaaacaaac gaggggcttt gggaaaagat aaaagggatt tttgtaaaat aatccttgcg
      661 ggtatcgtat agcggcaata cccaatcttc ccaagattgt gagaggggtt cgactcccct
      721 tacccgcttc atctttttta aatttttaaa aatccaacgt gttattaggt cacaattaga
      781 ttaataaaga tttttatttt ttatgccaac tttgcatcaa ttaataaaaa agccaagaaa
      841 agataaaaaa accaaaacaa aggttccggc tctttctttt ggttttaatc ccataaagaa
      901 caggcctact tattatccct caccatttaa aaggggtgtt tgtattaagg tttttactac
      961 gactcccaaa aagccaaact ctgctttaag aaaagtagca agggtgcgat tacaaaatgg
     1021 aatggaggta acggcttata ttcctggtga agggcataat ttgcaagaac attcgattgt
     1081 cttgattcgt ggaggaagag taaaggactt accaggtgtg cgttatcaca ttgtaagggg
     1141 tgtgttagat acagaggggg taaaaaacag aaagaaaaag cgttcaaaat acggagcaaa
     1201 aaaagggaaa taatctcttg tgtttagaaa ttaaaaattt aaaattagaa attaatttat
     1261 gcttcaaccc gatccaaaat ataataattt gattattcaa aaatttataa accagacaat
     1321 gcgtcaaggt aaaaaaacct tggctcaaag tattgtttat aacgcttttg atattattca
     1381 agaaaaaacc aaaaagaatc ctttggaggt ttttagccag gccttgaaaa atgccgctcc
     1441 tcatatggag accaaaccaa gaagagtagg cggagcaata tatcaggtac ccatagaagt
     1501 aaaaggagat aggcaaagga cattagctat gagatggatt ttggatgctg cacgatccaa
     1561 aaagggaaaa tcaatggatg aaaaattggc agaagaattg attttggctt caaaaaatga
     1621 gggtgcagct attaggaaaa aaataaatgt tgaaagaatg gcagaagcaa acaaggcctt
     1681 tgcgcatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat cctatcgaaa
     1801 gatatcgtaa cattggattt attgctcaca ttgatgctgg aaaaacaaca gtaagtgagc
     1861 gggttttatt ttacacagga gtttctcata aaattggcga ggttcatgaa ggccaggcaa
     1921 taatggattg gatggttgag gaacgtgagc ggggtattac cattacttct gcggcaacta
     1981 cttgcttttg gttacctact tatttaaaaa aagaagaagg agaaatggaa ccagaagaaa
     2041 gaaaatctaa agaataccag attaacgtta ttgatacgcc cggccatatt gattttacag
     2101 tcgaagttca aaggtcattg cggatactag atggagcagt agtaatattt gatggtgtct
     2161 ctggtgttga accccaatca gaaacagtat ggcggcaagc tgataagttc catgtgcctc
     2221 gtttatgttt tgtaaacaaa attgatcgca cgggtgctga ttttaaaaaa gatgttgaaa
     2281 gtattaaaac aaaacttacg cctaatgttg ttattcttca aatgcccctt gggcaggaag
     2341 aaaattttca gggagtgatt gatcttttat cattaaaagc atatttattt gagggagagc
     2401 atggggaaaa tattaaagaa gtagaaattc ctttggagtt aaaagacgaa gctatctctt
     2461 tgcaccaaca aaccattgaa aagattgttg agcaagacga ggcattaact gaaaaatatc
     2521 ttgaaggcaa tatacctgac ctgaatgaat taaagaaagt gttaagaaaa tcagtgattg
     2581 attttaagct ggtgccagtg ctttgcggct ccgcattaaa aaacaagggc gtgcaattat
     2641 tgcttgatgc tattgttgat tatttgccaa gtcctattga tctgcctcca gcgaagggag
     2701 agaatccaaa aacagggaaa gaagagataa gagagccaaa agacaatgct cctttttcag
     2761 ctcttgtttt taaagtggca agtgatcctt acgtgggaga gttgtcctat tttagagttt
     2821 actctggtac attgaaaaaa ggcagttatg tgcttaatcc taccaataat acaaaagaga
     2881 gaattggtcg gcttttgcga atgcattcca atgaaagaat tgagatagat gaggtttttg
     2941 ccggtggcat tgctgctact cttggactta aagataccaa aaccggtgat actctatgcg
     3001 acgaaaatgc tcctattttg cttgaaaaaa ttacctttcc agaaccagtt atttccattt
     3061 ctgttgagcc taataccaaa gaagatcaag aaaaattagc gctttccctc caaaaattag
     3121 caaaagaaga ccccaccttt aaggtaaaaa cagatgaaga aactcagcaa accattattt
     3181 ctgggatggg tgaattgcat ctggaaatta ttcttgatcg tttgagaaga gaatttggag
     3241 taaatatcaa ctctggcaga ccccaggtgg cttatagaga aacaatatca ctttccagtg
     3301 aagccgaggg taaatatatt cgtcaatccg gtggaagagg acaatatggt catgtctttt
     3361 taagggttga accaaaaaag cgaggagagg gatttgaatt tgtagatgaa attaagggtg
     3421 gagccattcc taaagaattt attccagcag tgaaaaaggg agtgattgaa gccagggaca
     3481 aaggaattat agctggtttt cctttaactg atataaaagt gacgctttac gatggaactt
     3541 ttcacgaggt tgattcttct gattttgctt ttaaaattgc tggatcaatt gctctttctg
     3601 aagcagttaa aaaagcaaaa ccagttttgc ttgaacctat tatgaaaaca gaagtgaggt
     3661 gtcctgaaga gtttttggga gatgtaattg gcgatttatc gtctcgcagg gcaaggatcg
     3721 aggagacaaa aagtatttta aatataaagg ttattgacgc ttatgtgccg ttatctgaaa
     3781 tgtttggtta tgccacagtt ttgcgctcct tgactcaggg aagaggaacc tttacaatgg
     3841 aatttgatca ttaccaagaa gttcctcaaa gcgttgctca acaaataatt gaaggaaaaa
     3901 aataatatat ttatagtatt tatggattta aagtctattt cttataatat attgcaatta
     3961 gccgaagaaa aaggagtttc tgaagaaaag gtgattgaga ttattgaaaa ggccctagcc
     4021 gctgcttata aaaaagagta tggtaataag agtcagcggt acggggctca ttttgacaaa
     4081 gatagtggta aggttgaatt tttcttaatt aaaaaggtcg ttaatgaaga tatgcttttg
     4141 cccgcaaatg cttcagtagc gcaggcagat tcaaaaaaag aaggtaagga ggaaaaaaaa
     4201 tcaaataaag aaacaactac tgataaagta attgaagaag aaggagagga acaaaaaaag
     4261 tttaaattta atcctgagcg tcatataatg ctgaacgagg caaaaaagat aaaaccagat
     4321 gtaaaaccag gagatcagtt agaatttcct gtgccttcct tggaaaaatt tggccgtatt
     4381 gctacccaga ccgcaaagca agtgttgttg caacaaatca gagaagcaga aagagaaagt
     4441 attctacaag aatttcagaa aaaagaagga gagattgtct ctggcgttat tcaacggaga
     4501 gaaggagata ctatctttgt tgatcttggt aagacaatag gaatcttgct gaaagaagat
     4561 caaattccag gtgagtttta taaattgggc aacagattga ggtttttttt attaaaagtg
     4621 aaggactctt cccatgggcc tgaaatttat ctttcacgcg cgcatcccaa atttttatct
     4681 aagctttttg agctggaagt gccagagatt gcgtctggca gtgttgaggt aaaggccgta
     4741 gcgcggcaag cgggttctcg ttcaaaaatt gcggtgcact cgaaagaaga aggcattgat
     4801 ccagtgggtt cttgtgtagg cgcaagaggc agtcgggtaa atgctgttat gcaagaatta
     4861 ggaagagaaa aaattgatat tattgctttt tctgaaaagc cagaagaatt tattgagaat
     4921 gctttgtcgc cagcaaaggt ggtaaatgtg acgattaacg aaaacaaggc cgtagtagaa
     4981 gtgcccaaag atcaattatc cttggcaatt ggcagagatg gtcagaatgt aaaactagct
     5041 tctgatttga ctggttggaa gatagatgta gttcctgcta atattgatga atctgataaa
     5101 aaagaagcaa ataaagaaaa aaaagcatca gttaaagagc caaaagaaga ggagaaaaaa
     5161 actgaagaaa aaacagagaa aagcaatgat aataaaaaaa gtcaaaagta aaataaataa
     5221 cctagaatta tcattttcat tatcgaagat gataatatct tattttttat gtcaataaaa
     5281 gtttttgttg aaataccaaa aggttcctcg caaaaatatg aatttgacga ggaagaaaaa
     5341 attataaaat tagacaggac cctttacagt ccaatgtatt ttccttttga atatggtttt
     5401 ataaagggga caatgggagg agacggtgat cccttggatt gtattttgtt ggcttcttct
     5461 cctacttttc caggatgttt ggtagaagca gaacctattg gacttttaga gatggaggac
     5521 gagggaggaa ctgataccaa gattattgca gtacccacca caaagattga tccacgattt
     5581 gaaggcatta atgacgtgaa tgatatttca gaacatacca agaaagaaat taaggagttt
     5641 ttcgagaact ataaaaattt agagccaggc aaatgggttg agctaaaggg ttttaggcct
     5701 aagcacgaag cagaaaaatt gatcgagaaa tcgtcaaatc tttaaaaact tcagagtttt
     5761 gataagtaaa aagaagttta ttttcgttct gctattacaa tactcgattt ttaattttac
     5821 attccgcttt tttatttatg cctgagctac ccgaggttga aaccattgta agagaattaa
     5881 gaaagaaggt ccttaaaagg accttctttg atatgtggac agacaatccc aaaatgatct
     5941 atttttgcaa tgattttaaa aaacaggtga aagacctaag agatattaat gagtttaaaa
     6001 aagaaattaa gggaaaagtc attaacaata tttggcggcg ggggaaaaat attatttttg
     6061 atctttcaaa aaataaatct ttgctgattc accaaaaatt aactgggcat ctgcttgtgg
     6121 ggcaatggac attaaataaa gggctttggg agccgatcga taataatttt ttaaaagagc
     6181 cagttaatcg ttatattcat ctaatgtttt ggttgagtgg agatcttatg cttgctcttt
     6241 ctgatttgcg aaagtttgcc aaggtagaat tatgggatag tgataaatta aaaaaatcaa
     6301 gagaattaaa tgaactaggt ccagagccgt tagacaagaa cttcactttt caaatattta
     6361 agaatgtttt gcctaaaaag ggtaagataa aacaagtttt aatgaatcaa accgttgttg
     6421 ccggtattgg caatatttac tcagatgaga ttttgtggga agcaagagtt tatccgctca
     6481 aagacgtatc aaaactttcc gaaaaagaac taaatgaaat ctatcaagca ataaaaaaga
     6541 ttttaaaaac agccattaaa ttgaaagggg aatcgttttc tgattttagg cgcacttcag
     6601 gagaaaaagg aaattttgat agagagagaa aggtttatca aaaagaagga gaaaaatgtt
     6661 ttcgttgcgg agcaactatt caaagagtta aattaggagg caggtctgct catttttgcc
     6721 ctgtttgtca aaagagcgat taatgattaa ttttaaaaaa cgaactttat aatattttaa
     6781 gttgcaagtt tttctaaaat tttctaaaat taaata
//
LOCUS       DAOTQD010000027         6582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_10073_length_6582_cov_8.836219, whole genome shotgun sequence.
ACCESSION   DAOTQD010000027 DAOTQD010000000
VERSION     DAOTQD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10073_length_6582_cov_8.836219"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1100)
                     /locus_tag="P5570_01775"
     CDS             complement(174..1100)
                     /locus_tag="P5570_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRR39464.1"
                     /translation="MTSWQDIQKEIAEGRAPDKPAGDFDGVRRGKYKLLSEITKRPLL
                     VYATAFHNPIKAQFAAPFLSIDLSDKDGFYEIIHNVKAKEVDVFVHSPGGSAEATESI
                     VKMLRKKFETIRFIVTGAAKSAATMLALSGDSILMDSSAELGPIDPQVRVRGRFSPAG
                     SIIEQFDRAAKELKKDPSKLPVWIPILQEFAPSLLVECENFIGLSNRLVTNWLIDYMF
                     KGEKDAKKKAKKIADYLTDEKNTLSHARRIDAEQLQKLGVKIEMLEEQDAKLQDAVRQ
                     LHFSVMATLDGTGAAKIFENSEGAVLIRMVNI"
     gene            complement(1107..1301)
                     /locus_tag="P5570_01780"
     CDS             complement(1107..1301)
                     /locus_tag="P5570_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39465.1"
                     /translation="MKRQKQAQKIGGQMKDFLKENPAIKEALRVFDISYDQYQKAIEG
                     GYGYYTDTSTSPQKVKFKTK"
     gene            complement(1356..2759)
                     /locus_tag="P5570_01785"
     CDS             complement(1356..2759)
                     /locus_tag="P5570_01785"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRR39466.1"
                     /translation="MATKITTIYASEIELTARLEPRFFYNQSLLKKFFDDYGFEELND
                     FASLKSGSTPEHFEERKNKDDLYFIKSADVKRYNLNFSTISFVSRDIHYSRKKFKVIP
                     NDVLLSNTGKYLGLACLIPKTIAEATTNQNIVRIRFREKACTRFNPFFILSFLNSLFG
                     QIEIESLLTLTGQKYLNMEKFREFKIPKINEKGIKKISKKTKAIIENEIKSLSILEQT
                     QGLFYQKLGLDFSKIEKQKFYSVKLSDFREADLWTPIYSYPFYVNILKTIQEKWETVP
                     LGEIATAKKGDEVGSDNYNKYLDKKDSDVPFIRTSDIVNYEVDQLPDFYIPEEIYQEL
                     KQDVKTGDVLFNNDGKIGLVAMLTPQDKVILQSHIKRLRLKKEAIEKYHITQEYLFLV
                     LTIKEIARCQAERYTVIQSTIPTISNRILDFEIPILDKVSIDEITKLVKEAFELKDEK
                     KKLIREVREEIDSYFGI"
     gene            complement(2769..4589)
                     /locus_tag="P5570_01790"
     CDS             complement(2769..4589)
                     /locus_tag="P5570_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRR39467.1"
                     /translation="MSKTNYKPIKEEIKAEPEKENAGYVRDYITNTWVKATPEEIEAV
                     QIFVKQLVEDYNYPKAHIQTRPQYRVKQRPSGGKDFPVDIAVFSSNNHTFDNEYILVE
                     CKKKNRKDGKSQLEDYLRFSKAELGVWFNGEERLFLRKIEKQGKIFFEEIPNIPQYGQ
                     RVEDIGKFKRQDLRPTHNLKATFKAIRNHLAANTVGATRDEVLAQQLINLIFCKIYDE
                     RFTERDDIVTFRAGVDEETKEVKERILELFEKVKRKYKEVLDESDTITLDANSIAYVV
                     GELQNYCLIEAERDIIADAFETFIGHALKGGQGQFFTPRNVVKMMVDILDPDDEDLII
                     DPACGSGGFLIEALRHVWRKLDSEGEKYHWNYENLKEEKMEFALNRIRGIDKDYFLAK
                     VAKAYMTIIGDGKSGIVCEDSLENPQNWSDATRQKIHLGNFSVLLTNPPFGSKIPVRG
                     EDKLKQFDLGHKWKLNKKTNEWEKGKLKDKEAPQILFIERCLQLLKNGGRMAIVLPDG
                     IYGNNQLGYIRSWLLERARIIAVVDVPVETFMPNTTTKTTIMVLQKLPKNKIPKDYPV
                     FMAVAETCGHDRRGNIKEDDDIARIPEEFRKWAKENKFSF"
     gene            complement(4586..5467)
                     /locus_tag="P5570_01795"
     CDS             complement(4586..5467)
                     /locus_tag="P5570_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002480163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HRR39468.1"
                     /translation="MPIFQTENNKIRQLKTSNFRNEKELQTLVENNLEEIFGVKFIAS
                     ELSTGEKHGGRIDTLGLDENNSPVIIEYKWGEKDNVINQGLFYLDWLVDHKGDFQILA
                     EKKLKKIKTDWSQPRLILIASAFNKYDKYAINRMSENIELWTYTLYENGIFEVNIEGS
                     SQASDKKGKRITKIEYAKYDLNYHLNKTSKDLQKKFGEIREKILEWSNVQEKPDQKSG
                     VTYKTTKSFVRFEFGKNYINALVRNSRYGHTIDSEGMVKDITAFEWGYKGLIKITLET
                     DVNYLLDLIRQSYEETL"
     gene            complement(5472..6155)
                     /gene="radC"
                     /locus_tag="P5570_01800"
     CDS             complement(5472..6155)
                     /gene="radC"
                     /locus_tag="P5570_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRR39469.1"
                     /translation="MSKSFTIHDLPKDERPRERLVKFGEQALSAQELLQLILGRGVAG
                     ESVAITAQKLLAQFGSLQKLAEASIEELSSIKGIGLAKAAQIKATFEISRRLSTQVPT
                     YKSKELTDPEKVYRLIKGKLKDYHKEHFYIIALNSRNYSIAEVSVGSLNTSVVHPREV
                     FAEAIKNKAASVIFAHNHPSGDPEPSEDDLEITKQLTESGKILGIEVIDHIIITKTGF
                     TSFKEKNLI"
     gene            complement(6160..6369)
                     /locus_tag="P5570_01805"
     CDS             complement(6160..6369)
                     /locus_tag="P5570_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRR39470.1"
                     /translation="MLREISQIGKNIKKLRKQKGLSQDRLSKLADISYNTVIKLESGG
                     ITNPSIDTLQKLAKALNVSVDNLLK"
BASE COUNT         1782 a         1246 c         1071 g         2483 t
ORIGIN      
        1 caattactga acgaagcgcg aacctttttt gagcaagaat ttttaccctg acgggcagaa
       61 atcccgctcg tccccatctg gggtgggaaa ccttcccgct tcgtgggcga gccgatgatg
      121 attttttatt gttggttgga ttgaggtatt tggggtggca taatttgaac tggttatata
      181 ttcaccattc ttattaaaac agccccttcc gaattttcaa aaatcttagc cgctcccgtt
      241 ccgtccaaag tagccatcac tgaaaaatgg agttgacgaa cagcgtcttg aagtttcgcg
      301 tcttgctctt ccaacatctc aatttttacc cccaattttt gtagttgttc ggcatctatc
      361 cttctcgcgt gagaaagagt gtttttctca tcagttaggt aatccgcaat ctttttggct
      421 ttcttctttg catctttctc acccttaaac atataatcta taagccagtt agtaacaagt
      481 ctgttagaga ggccgataaa attttcgcat tctaccaaaa gcgatggggc gaattcttgt
      541 agtattggta tccaaacagg caattttgaa gggtcttttt ttaattcttt tgccgctcgg
      601 tcaaactgct caataataga gccagcagga gaaaatcgcc ctcgcactct aacttgtggg
      661 tcaattggtc ctaattcagc cgaactgtcc attaaaatac tgtcgccaga taaagccaac
      721 attgtggccg cgctcttagc cgcccccgta acaataaacc taatcgtttc aaatttttta
      781 cggagcattt taacaataga ttctgttgcc tcggcagagc cgcccggact atgtacaaag
      841 acatcaactt ctttcgcttt tacattatgg attatctcat agaaaccgtc tttgtctgaa
      901 aggtcaattg atagaaatgg tgcggcgaat tgggctttaa taggattatg aaaagctgtg
      961 gcataaacaa gaagggggcg tttagtgatt tcgctcaata atttgtattt ccctcttctt
     1021 acaccatcaa aatctccggc tggtttatcc ggtgctctgc cttcggctat ttctttttga
     1081 atatcttgcc aacttgtcat atgattttac tttgtcttaa atttgacttt ctgtggtgag
     1141 gtagaggtat cggtatagta gccatacccg ccttcaatcg ctttttgata ttggtcgtag
     1201 gaaatatcaa aaacccgcaa tgcctcttta attgcagggt tttctttcaa aaaatccttc
     1261 atctgaccac ctattttttg ggcttgtttt tgtcttttca tagtgtcttt attatacctc
     1321 cattttataa tttcctcaac cttttcagtt gtttcttaaa tcccgaaata gctgtcaatt
     1381 tcttctctaa cttctctaat cagttttttc ttttcgtctt tcaattcaaa cgcttctttt
     1441 actaattttg taatttcatc aattgaaact ttatctaaaa tagggatttc aaaatctaaa
     1501 atgcgatttg agattgttgg tattgttgat tgaattaccg tatatctttc agcttggcat
     1561 ctggctattt ctttgattgt caaaactaaa aataaatact cttgtgtaat gtgatatttt
     1621 tcaatcgctt ccttttttaa tctcagtctt tttatgtggc tttgtaaaat aaccttatct
     1681 tggggagtca acatcgccac aagtccgatt ttcccatcat tattaaaaag cacatctcca
     1741 gttttaacat cttgctttaa ttcttgataa atctcttctg gaatataaaa atcagggagt
     1801 tgatcaactt cataatttac aatatcggaa gttctaataa atggaacatc gctatctttt
     1861 ttgtctaaat atttattgta attatcgctt ccgacttcat cgcccttttt ggctgtggcg
     1921 atttcgccaa gcggaacagt ttcccacttt tcctgaattg tttttaatat atttacataa
     1981 aatgggtaag aatatatggg ggtccacaaa tcggcttctc taaaatcaga aagttttaca
     2041 gaataaaatt tctgtttttc tatttttgag aaatcgaggc ctagtttttg atagaaaagg
     2101 ccttgtgttt gttctaagat tgaaagggat tttatttcgt tttcaatgat tgcttttgtt
     2161 tttttgctta tctttttaat acccttttcg tttattttag ggatcttaaa ttccctaaac
     2221 ttttccatat ttagatattt ttgacctgta agtgtaagta gtgattcaat ttcaatttgt
     2281 ccgaataatg aatttaaaaa agatagaata aaaaaaggat taaaccttgt acatgctttt
     2341 tctctaaatc taatccttac aatattttgg tttgtcgttg cttcggctat ggttttaggg
     2401 attaaacaag caagtcctaa atattttccc gtgtttgata ataaaacatc atttggaatt
     2461 actttaaatt tttttcttga gtaatgaatg tctcttgaaa caaaactaat tgttgaaaaa
     2521 tttaaattat accttttgac atcagcggat tttataaaat aaaggtcatc tttatttttt
     2581 ctttcttcaa aatgctctgg tgttgaaccg ctttttaagc ttgcaaaatc atttaattct
     2641 tcaaatccat aatcatcaaa aaattttttt aataaacttt gattataaaa aaatcttggt
     2701 tctaatcttg cagttaattc tatttcagaa gcataaattg ttgtaatttt tgttgccata
     2761 ttcaaatctt aaaaactaaa tttattttct tttgcccatt ttctaaattc ttcaggtatt
     2821 ctggcaatat catcatcttc ctttatgtta cctcttctgt cgtgtccgca agtttcggca
     2881 actgccataa acacgggata atctttagga attttatttt taggtaattt ctgcaaaacc
     2941 ataatagtag ttttagttgt agtgttgggc ataaatgtct ctacgggaac atcaactacg
     3001 gctattattc ttgctctttc aagtagccag cttctgatat atcccagctg attattgccg
     3061 taaattccat ctggtaaaac aatagccatt cttccgccat tttttaataa ctgtaaacac
     3121 ctttcaataa atagaatttg tggggcttct ttatctttta attttccttt ttcccattcg
     3181 tttgtttttt tatttagctt ccatttatgt ccaagatcaa actgttttaa tttatcttcc
     3241 ccgcgaacag gaatttttga accaaaagga ggattggtta aaagaacact aaaatttcct
     3301 aaatgaattt tttgtctggt tgcatcactc caattttgcg gattttctaa actatcttcg
     3361 cagacaattc cgcttttgcc gtcgccgata attgtcatat atgcttttgc gacttttgcc
     3421 aaaaaataat ctttgtcgat gcctctaatt ctatttaaag caaactccat tttttcttct
     3481 ttaagatttt cataattcca atgatatttt tctccctctg agtcaagttt tcgccaaaca
     3541 tgtcttaatg cttcaatcaa aaatccacca cttccacaag cagggtcaat aattaaatct
     3601 tcatcatcag gatctaaaat atccaccatc atttttacaa catttctcgg agtgaaaaat
     3661 tgtccttggc cgcctttcag ggcgtggccg atgaatgttt caaaagcatc agcaataata
     3721 tctctttctg cttcaattag gcagtaattc tgtaattcac caaccacata agcaatagaa
     3781 tttgcgtcta gtgtaatagt gtcgctctcg tctaagactt ctttatattt cctttttact
     3841 ttctcaaaaa gttctaaaat tctttctttt acttcttttg tttcctcatc aacgccggct
     3901 ctaaaagtaa caatatcatc tcgctcagta aatctttcat cgtatatttt acagaaaatt
     3961 aagtttatca attgttgagc caaaacttca tccctcgttg ccccaacagt gtttgcggct
     4021 aagtggtttc tgattgcttt gaaagtagct tttagattat gagtgggcct aagatcttgt
     4081 cttttaaatt ttccaatatc ttctactctc tgtccatact ggggaatatt tgggatttct
     4141 tcaaaaaata tctttccttg tttttcaatc tttcttaaaa ataatctttc ttctccgtta
     4201 aaccatacgc ccagttccgc ttttgaaaat cttagataat cttcgagttg tgattttcca
     4261 tcttttctat tctttttctt acattcaaca agaatgtact cattatcaaa ggtatggttg
     4321 tttgaagaaa agacggcaat atcaacagga aagtctttac cccctgatgg tctttgtttc
     4381 acgcgatatt ggggtctggt ctggatatgt gcttttggat agttatagtc ctcaactaac
     4441 tgttttacaa aaatttgaac agcttcaatt tcttcgggag tagccttaac ccacgtatta
     4501 gtaatataat cgcggacata tcccgcattt tctttttccg gttcggcttt aatttcttct
     4561 tttattggtt tgtaatttgt tttgctcata gtgtttcctc gtaagattgt ctaattaaat
     4621 ctaaaagata attaacatct gtttcgaggg ttatttttat caaaccttta tatccccatt
     4681 caaaagcagt tatatctttt accatacctt ctgaatcaat cgtgtggcca tatcttgaat
     4741 ttctgacaag ggcattaatg tagtttttgc caaattcaaa tctgacaaag ctctttgtgg
     4801 ttttgtaagt aacgccactt ttttggtccg gtttttcctg aacattagac cattctaaaa
     4861 ttttctctct tatctctcca aattttttct gtaaatcttt tgaggttttg ttgaggtgat
     4921 aattcaaatc gtatttcgca tattctattt tcgttatgcg cttgcctttt ttatcgcttg
     4981 cttgcgagct cccctcaata ttaacttcaa aaataccatt ttcgtataaa gtgtatgtcc
     5041 aaagttctat gttttcggac attctgttga tagcgtattt atcatacttg ttaaaagccg
     5101 aagcgattaa aataagtctt ggctggctcc aatctgtttt tatttttttc aatttctttt
     5161 ctgcaaggat ttggaaatct cctttatggt caaccagcca atcaagataa aagaggccct
     5221 gattgataac attatctttt tcgccccatt tgtattcaat gataactggc gaattgtttt
     5281 cgtctaatcc caatgtatca attctgccgc cgtgtttttc gccagtagaa agttcggaag
     5341 cgataaattt gaccccaaat atttcttcta aattattttc aactaaagtt tgtaactctt
     5401 tttcattcct gaaattagag gtttttaatt gtcttatttt attgttttcg gtttggaaaa
     5461 ttggcatagg attaaattaa atttttctct ttaaaacttg taaatcctgt ttttgtgata
     5521 attatatggt caattacttc aatccctaaa atcttaccgg attctgttaa ttgttttgtt
     5581 atttccaaat cgtcttccga tggttcagga tcgccagatg ggtgattatg agcgaatata
     5641 accgaggcgg ctttattttt aatggcttct gcgaatactt cgcgtggatg gacaacactt
     5701 gtattaagac tgccaaccga aacttctgca attgaatagt ttctgctgtt aagagcgata
     5761 atataaaaat gttccttgtg ataatcttta agcttacctt ttatcagtcg ataaactttt
     5821 tccgggtcgg taagctcttt gcttttataa gtcgggactt gagtagaaag ccgacggcta
     5881 atttcaaatg tggcttttat ttgagcggct tttgctaagc caatcccttt aattgaggat
     5941 agttcttcga tgcttgcctc ggctaatttt tgaagactgc caaattgcgc taatagtttt
     6001 tgggcagtaa ttgctactga ttcgccggca acgcctcggc ctaaaataag ctgtaataat
     6061 tcttgagccg agagtgcttg ctctccaaat ttaactaacc gttcgcgtgg acgctcatct
     6121 tttggcaagt cgtgaatagt aaatgatttg ctcatattct tacttcaata agttatcaac
     6181 agaaacatta agggctttgg ctaatttttg aagcgtgtca attgagggat tcgtgatacc
     6241 gcccgactcc agctttatca ccgtgttata ggaaatatca gccaatttag aaagccggtc
     6301 ttgtgacaag cccttttgtt tccttaactt ttttatattt ttacctattt gcgaaatctc
     6361 tcttagcata gtagtattaa aataattgta tgtacgtata gagtagtcct tatctataat
     6421 tatgatacaa aattattttg ataatgcaaa gagaaattta gggagtttaa tttcaaatgc
     6481 ccgcgcacgg gagggctggg gcaagggcat ttaccttttt tgttttctaa aaatcatcgg
     6541 ctcgcccgca aagcgaagcg gaggcgggcg ggcaaaaaga aa
//
LOCUS       DAOTQD010000028         6452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_10336_length_6452_cov_7.551821, whole genome shotgun sequence.
ACCESSION   DAOTQD010000028 DAOTQD010000000
VERSION     DAOTQD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10336_length_6452_cov_7.551821"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /gene="rplV"
                     /locus_tag="P5570_01810"
     CDS             complement(<1..314)
                     /gene="rplV"
                     /locus_tag="P5570_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRR39471.1"
                     /translation="MEIKASVNYLRIAPRKTRLVVDLVRGMDVDKAKAVLQFAEQKSA
                     RPILKLLNSALADAENNYQLNKEVIKNLYIKSITVDEGPKLKRWMPVSRGTAHQIQKK
                     TS"
     gene            complement(342..626)
                     /gene="rpsS"
                     /locus_tag="P5570_01815"
     CDS             complement(342..626)
                     /gene="rpsS"
                     /locus_tag="P5570_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRR39472.1"
                     /translation="MSRSLKKGVYCDPKLEKKIKEYRNKPIKTWARSTMITPEMIGID
                     FLVHNGKEFIAVRVREEMVGHRLGEFAPTTRFQKHGGKMERELSKKEGSK"
     gene            complement(668..1492)
                     /gene="rplB"
                     /locus_tag="P5570_01820"
     CDS             complement(668..1492)
                     /gene="rplB"
                     /locus_tag="P5570_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRR39473.1"
                     /translation="MMKKKKPTTPGQRGMISQDYSKLTKKKPEKRLVLSFKKNAGRSN
                     TGRITVRHQGGGEKKKYRIVDFGQEKVNIPAKIEALEYDPNRSAFLMLLLYQDGERRY
                     QLAPEGIVVGDSIICKEDAPLKEGNRLKLKNIPGGTFVFNVELIPGRGGKIAKSAGNT
                     AQILSKEEGYATLKLPSKEVRLISEECFATIGQVSNADKKTEVIGKAGKKRHRGIRPT
                     VRGTVMNPCDHPHGGGEGRTPIGLKHPKTPWGKPALGKKTRKKHKFSNRYIVKRRK"
     gene            complement(1566..2018)
                     /gene="rplW"
                     /locus_tag="P5570_01825"
     CDS             complement(1566..2018)
                     /gene="rplW"
                     /locus_tag="P5570_01825"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRR39474.1"
                     /translation="MVTLPFQRKKEERNYKEKIKNDTKKQKPSLKQKQQKKVVSKSST
                     KPSTKKTKELGKSKIAYRVLKEPIISEKGTRLAEEGKYFFKVFDNANKVEIKRAIEDV
                     YRVKVENVNIVNIPRKKKRLGRQEGWKKGYKKAVISLKKGDKIETSPR"
     gene            complement(2051..2680)
                     /gene="rplD"
                     /locus_tag="P5570_01830"
     CDS             complement(2051..2680)
                     /gene="rplD"
                     /locus_tag="P5570_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRR39475.1"
                     /translation="MKSALYNQKGEKVGEVDLPEDVFKQKINPDHIYQAVLYYENLKR
                     KPIAKTKDRSEVRGGGRKPWRQKGTGRARHGSTRSPIWKGGGVTFGPSPEKFSQIKMS
                     KKARKRAFEMVFSKKLEEKEIIFLDQLTIDKLKTKSAEKIIKNLTKIAPGIDKKKTGI
                     ALKKADENIKKAFRNIKNIEIYNVSAINAYFLLQNKYIIIEEKAVNELF"
     gene            complement(2779..3375)
                     /gene="rplC"
                     /locus_tag="P5570_01835"
     CDS             complement(2779..3375)
                     /gene="rplC"
                     /locus_tag="P5570_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRR39476.1"
                     /translation="MKFILGKKIEMSQMFDEKGRVIPLTWIEAGPCVILQIKNKEKEG
                     YEAIQIGFKKKSKNIKKTEKGKEYKYIKEFPVFESQPLMKVGEIIDVSVFQEGDKVKI
                     SGTSKGKGFQGGVKRWGFRGASKTHGTKHMWRKPGSIGATDAERVFKGKKMAGRMGGD
                     RVTLKNLEIVKIDKEKNLLGIKGAVPGKRGTIIEIIQE"
     gene            complement(3811..4170)
                     /gene="rpsJ"
                     /locus_tag="P5570_01840"
     CDS             complement(3811..4170)
                     /gene="rpsJ"
                     /locus_tag="P5570_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRR39477.1"
                     /translation="MPAKTESNIEDKKQAKEATPLRLRIKVKAYDHRVIDSSVRQIVE
                     NALRQGAQVVGPIPLPTEIKRFTVNRSTFVHKDAREQFEMRVHKRMIDILNPNRRIID
                     TLQNLNLPSGVDVEIKM"
     gene            complement(4172..5374)
                     /gene="tuf"
                     /locus_tag="P5570_01845"
     CDS             complement(4172..5374)
                     /gene="tuf"
                     /locus_tag="P5570_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRR39478.1"
                     /translation="MAEKQKFERKKPHLNVGTIGHVDHGKTTLTAAILHSLKLAGFIA
                     SEKTIEQIDAAPEERQRGLTINITHVEYESEKRHYAHIDCPGHADYIKNMITGAAQMD
                     GAILVVSAPDGPMPQTKEHILLARQVGVPSIVVFLNKIDQVDDPELVDLVEEETRELL
                     TKYGFDGKNIPVVRGSALKALEATSKDDEAIKPILELVKALDENIPEPQRPIDQPFLM
                     AVEDVFTIEGRGTVATGRIERGVVHLNDEVELVGIKPTKKTTVTGIEMFNKILDEGRA
                     GDNVGILLRGLKKEEVLRGQVLAKPGTVTPHTEFECQVYILSTKEEGGRHTPFFNGYK
                     PQFYIRTADVTGEVTLPQGTEMVMPGDTVNLTVKLITPAAMEEKQKFAIREGGKTVGA
                     GTITKIIK"
     gene            complement(5605..5970)
                     /locus_tag="P5570_01850"
     CDS             complement(5605..5970)
                     /locus_tag="P5570_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR39479.1"
                     /translation="MSKILIVEDDKFLRELLESKLKEEKDFTVVTAKDGEEGLQKARE
                     EDPDLIILDLILPGFDGFEVLEKIKSSAATVDTKVIVLSNLGQKEDIDRAMKLGAEDY
                     LIKAHFTLEEIVKKIRKNL"
     gene            complement(6013..6384)
                     /locus_tag="P5570_01855"
     CDS             complement(6013..6384)
                     /locus_tag="P5570_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRR39480.1"
                     /translation="MAQKILLVEDEKILADLLEKKLKEENYKVSIAPNGQAGLSKMKK
                     EDFDLVLLDVLMPKKGGFEVLEEMQADPRLKKIPVIIISNSGQPVEISRALEMGARDY
                     LVKTQFDPKEVIEKVKKTIGE"
BASE COUNT         1729 a         1301 c          836 g         2586 t
ORIGIN      
        1 tgacttgtct ttttttgaat ctgatgggct gtgccccttg aaaccggcat ccatcttttt
       61 aattttggtc cttcgtctac agtaatactt ttaatataaa gatttttaat aacttccttg
      121 ttcaattgat aattattttc cgcatcagcc aaagctgaat ttaataattt taaaatgggc
      181 ctggctgact tttgttcagc aaattgaagt actgcctttg ctttatcaac atccattcct
      241 ctaactaaat caactaccag ccgtgttttc cgcggtgcaa tacgtaaata atttactgat
      301 gctttgatct ccataaaatt catctaaaat tacgaattgg tttatttact tccttctttt
      361 ttcgataatt ctctttccat tttaccacca tgtttttgaa atcgtgttgt cggcgcaaac
      421 tcccctaacc gatgccccac catttcttcc cttaccctaa cagcaataaa ttctttacca
      481 ttatgtacca aaaaatcaat accaatcatt tctggtgtaa tcattgtaga acgggcccag
      541 gtttttatgg gtttgtttct gtactctttt atctttttct ctaattttgg atcgcaataa
      601 acaccttttt ttaaacttcg tgacatattt ttatatcaac gaatttacta attcataaat
      661 tcgctaatta ttttctacgt ttaactatgt atcgatttga aaatttgtgt tttttccttg
      721 tttttttacc taaagccggt tttccccatg gtgttttggg gtgttttaaa ccaatcggcg
      781 ttcgtccttc tcctcctcca tgcggatgat cacaaggatt cattactgtt cctctcacag
      841 taggcctgat tccccgatgt ctttttttgc ctgcttttcc tatcacttct gtctttttat
      901 ctgcatttga cacttgacct attgtagcaa aacattcttc tgatatcaat cgaacctctt
      961 ttgaaggaag ttttaaagta gcatatcctt cttcttttga taaaatctgc gctgtatttc
     1021 ctgcgctctt tgcaattttt cctcctctgc ctggaattaa ttccacattg aaaacaaaag
     1081 taccaccagg aatgtttttt aattttaaac gattaccttc ttttaaagga gcatcttctt
     1141 tgcaaatgat tgaatctcct accactattc cttctggtgc taattgatat cgtctctctc
     1201 catcttgata caaaagcaac atcaaaaaag ctgagcgatt gggatcatat tccaaggctt
     1261 ctatctttgc cggaatatta actttttcct gaccaaaatc cactattcga tatttctttt
     1321 tttctccacc gccttgatga cgaacagtaa tcctaccagt atttgatctg cctgcatttt
     1381 ttttaaaaga aagaaccaac cttttttcag gttttttctt tgtcagtttt gaataatctt
     1441 gactaatcat tcctctttgc cctggtgttg ttggtttctt ttttttcatc atatgaatat
     1501 gtaaataatt tattccttag atttatttta aacaaaattt aaaaacttga acctgtcttt
     1561 taatattaac gaggggaggt ttcaatcttg tctccttttt ttaaagaaat aactgctttt
     1621 ttatatcctt ttttccaacc ttcttgtctc cctaatcttt ttttctttct gggaatattc
     1681 acaatattta cattttcaac ctttaccctg taaacatctt caatcgctct tttaatttca
     1741 actttattgg cattatcaaa aaccttaaag aaatattttc cttcttctgc caatcgcgtc
     1801 cctttttctg aaataattgg ttcttttaat actcgataag caatcttact ttttccaagc
     1861 tcttttgttt tttttgttga gggctttgtt gaagatttag aaaccacctt cttttgttgt
     1921 ttttgtttta aagagggttt ttgctttttt gtgtcattct ttattttctc tttataattt
     1981 ctttcttctt ttttcctttg aaacggcaag gtaaccatat atttaatttt ttagaaataa
     2041 attaaaaaaa ctaaaaaagc tcgtttactg ctttttcttc tattataata tatttatttt
     2101 gtaataaaaa ataagcgttt atggcagaga cattataaat ctcaatattt ttgatatttc
     2161 taaatgcttt tttaatattt tcatctgctt ttttaagcgc aatgcctgtc ttctttttat
     2221 cgataccagg cgctattttt gttaaatttt taataatttt ctctgctgat tttgttttta
     2281 atttatctat tgttaattga tccaaaaaaa taatttcttt ttcttctaat tttttagaga
     2341 aaaccatttc aaaggctctt tttcgtgctt tttttgacat ttttatctga ctaaactttt
     2401 caggactagg accaaatgtt actccgcctc ctttccatat tggtgatctg gttgaaccgt
     2461 gtcttgctct gcctgttcct ttttgccgcc atggttttct tcctccgccc ctgacttcgc
     2521 ttctgtcttt tgttttggca atgggtttgc gtttaagatt ttcataatac aaaactgcct
     2581 gataaatatg atctggatta attttttgct taaaaacatc ttctggcaaa tcaacctctc
     2641 ctactttttc tcctttctga ttgtataaag cagatttcat aaatataact ccaatctatg
     2701 aatttattat ttattctgat gatccaaata agcgaatttg cttattttat agattggtac
     2761 tttatctgta gatttatatt attcttgtat gatttctatt attgttcctc tttttcctgg
     2821 cactgctcct ttgatgccta ataaattttt ctctttatca attttgacaa tctccaaatt
     2881 ttttaatgtc actctatctc cacccattct gccagccatt ttttttcctt taaaaactct
     2941 ttctgcatcc gttgcgccaa ttgaaccggg cttgcgccac atatgttttg taccatgagt
     3001 ttttgaagcg cctctaaacc cccaacgctt aacacctcct tgaaatcctt tcccctttga
     3061 agtgccagaa atcttaactt tgtctccttc ttgaaaaact gagacatcga ttatttcgcc
     3121 tactttcatc aatggttggg attcaaaaac aggaaattct tttatatatt tatattcttt
     3181 tcctttttcg gtttttttta tattttttga ttttttctta aaaccaattt gaattgcttc
     3241 atatccctct ttttctttat tttttatttg caaaataaca caaggacctg cctcaatcca
     3301 ggttaatgga atcacccttc ctttctcatc aaacatctgt gacatttcta ttttttttcc
     3361 taaaataaac ttcataaatc tataaagacg aaaataaact tcagtgatca accctaaact
     3421 catttcaggg aataaacaat aattttgtca tctcaagaaa taatagcaat gtaataacct
     3481 cgcggtattt tatttcttaa tcttatcaaa agttagaatc aagtttttaa cacaacatta
     3541 tacaacggga ttgcttcggt cggctatcgc ctccctcgca atgacatttg tggcgttgcg
     3601 ttaagttctt gaacctaaga aaagtaccaa tctgtttcag taactattat ctttgtaaat
     3661 aaaaatcggg cacagcccga tttttttcgt gctatccaaa ataaaatctt aaaaatataa
     3721 atacgatttt atcatcaata tttttattat aagtattatt aagatagatt ttaatcaagc
     3781 tgaatatctt tctgtattaa atcttaaata ctacatttta atctctacat ccactcctga
     3841 aggcaaatta agattttgaa gcgtatcaat aatccggcga ttaggattta atatatcaat
     3901 cattcttttg tgaactctca tttcaaattg ttctcttgcg tctttgtgca caaaggtaga
     3961 acgattaact gtaaaccttt ttatttctgt gggcaaggga ataggaccaa ccacttgtgc
     4021 tccttgacgc aaagcatttt caacaatttg tcttaccgaa ctatcaatta ctcgatgatc
     4081 ataggccttc accttaattc ttaaacgcaa aggtgttgcc tcttttgctt gttttttgtc
     4141 ttcaatattg ctttcagttt ttgcaggcat tttatttgat aattttggta atggttccag
     4201 ctcccactgt cttgcctcct tctctaatag caaatttctg tttttcttcc attgctgctg
     4261 gggtgattaa tttaactgtt aaattgactg tatcgcctgg cattaccatt tctgttccct
     4321 gaggcaaagt aacctctcca gtcacatctg ctgttctaat ataaaactgc ggtttgtaac
     4381 cattaaagaa cggtgtgtgt cttcctccct cttcttttgt tgataaaata taaacctgac
     4441 attcaaattc ggtgtgagga gttactgtgc cgggttttgc caatacttgc cctcttaata
     4501 cttcttcttt ttttaaccct cttaataaaa ttcctacatt atcaccagcc cttccttcat
     4561 caagtatttt attaaacatt tcaatgccag taacagttgt ttttttggtt ggctttattc
     4621 ctactaattc cacttcatca tttaaatgca ccacgcctct ttctattctt cctgttgcca
     4681 ccgttcccct tccttcaatc gtaaagacat cctcaactgc catcaaaaat ggctgatcta
     4741 ttggtctttg aggctcagga atattttcat ctaatgcctt taccaattcc aaaattggtt
     4801 ttatcgcttc atcatctttt gaagtggctt ctagcgcctt taacgctgat ccacgaacca
     4861 caggaatatt tttgccatca aaaccatatt ttgttaataa ctctcttgtt tcttcttcta
     4921 ccaagtcaac taattctggg tcatccactt gatcaatttt gttaagaaaa accacgatcg
     4981 atggcacacc cacctgacga gcaagcaaga tatgttcttt tgtctgaggc atcggaccat
     5041 caggtgctga taccaccaaa atcgctccat ccatttgagc agcgccagta atcatatttt
     5101 taatataatc agcgtgaccc ggacaatcaa tgtgggcata gtgcctcttt tcactttcat
     5161 attcaacgtg ggtaatattt attgttaatc cccgttgtct ttcttctgga gcagcatcta
     5221 tctgctctat agttttttcg ctagcaataa aaccagctaa tttcaatgaa tgcaatattg
     5281 ctgctgttag cgttgttttg ccatgatcaa catgaccaat cgttcccaca ttcaagtggg
     5341 gtttttttct ttcaaatttt tgtttctcag ccataattat tctaaatccc aagcaccaaa
     5401 aaccaaactt aaaacaatat ctaaatttca ataatcaaaa ctctaaatat gttttgaatt
     5461 ttgaatttaa aatttgtttg gagtttggaa tttggaattt ggtattttaa tttatttgta
     5521 ttatttactt tatttatttt taaaaattta ttaaggattt ttaattatat tccctttttt
     5581 aaaaaatgtc aaggataaaa ggtcttataa gttttttctt attttcttta ctatttcttc
     5641 aagcgtgaag tgcgctttta ttaagtagtc ctctgctcct aatttcattg cccgatcaat
     5701 atcttctttt tgcccaagat tggaaagcac aattactttt gtatcaacag tggcagcgct
     5761 tgattttatt ttttctagaa cctcaaaccc atcaaaccct ggtaaaataa gatctaatat
     5821 aataagatca gggtcttctt ctcttgcttt ttggagaccc tcttctccgt ctttggctgt
     5881 tacaactgta aaatcttttt cttctttcaa ctttgactcc aacagttctc ttaaaaactt
     5941 atcatcttct acaattaaaa ttttggacat aatttttata attaaacaac tgtttagttg
     6001 ctgattgtaa aattattctc cgattgtttt tttaactttt tcaatcactt ctttgggatc
     6061 aaattgggtt ttcaccaaat aatctcttgc acccatttca agcgcacggg aaatttcaac
     6121 cggctgacct gagtttgaaa taataattac aggaattttt tttaggcgtg gatctgcttg
     6181 catttcttcc agcacttcaa atcctccctt ttttggcatt aaaacatcca atagcaccaa
     6241 atcaaaatct tcttttttca ttttagaaag gcctgcttgg ccattcggag caatagaaac
     6301 tttataattt tcctctttta attttttttc caaaagatca gctaaaattt tttcatcttc
     6361 tacaagtaaa atcttctgtg ccataaataa attattattc tactattagt atcattttag
     6421 ctctcttttt caaaaagtca agagcaataa aa
//
LOCUS       DAOTQD010000029         6223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_10800_length_6223_cov_9.121757, whole genome shotgun sequence.
ACCESSION   DAOTQD010000029 DAOTQD010000000
VERSION     DAOTQD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10800_length_6223_cov_9.121757"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2009
                     /gene="acs"
                     /locus_tag="P5570_01860"
     CDS             81..2009
                     /gene="acs"
                     /locus_tag="P5570_01860"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRR39481.1"
                     /translation="MKFIKKGNLYYPPEDFKKQSLVKSKEIYKKAAEDPILFWENAAK
                     ELDWYKKWEKAFYFQPPYLQWFLGGKINLAQNALDRHLKTNKDKVALIWEPEPIEEKP
                     QIFTYNDLYQKVNQLANSLKRIGIKKGDRVAIYMPMIPEAIISMLACARIGAVHIVIF
                     SAFSPDALKIRLEISGAKVLITADGYYRRGKIIPLKINADEGAKGTLIEKVIVAKRLG
                     NKIPFNKEKDLYFDELIKNESTECEPEILDSEDPFFILPESGTTGQFLPILHTVGGYT
                     VQAHLSGKLIFDFQKDSVLWCTSDIGWVTGHTYTVYAPLLNGISTVIFEGAPDWPTPD
                     RWAQIIEKNKVSVFYTAPTAIRMFKKLDKKVLQQYQFKNLRVLGSVGEPMDEATWLWY
                     FENIGKERCPIVDTYWQTETGGIVISSLPGVGPFRPGFCGLPFPGIKVDILDSKGKPC
                     LVGERGNLVVLPPFSPGFLRGIYNDEQKYVETYWNQYGKEIYFTSDGAVRDENGLIKI
                     TGRIDDVMKISGHRLSTNDLESVILKHSDIIETAVIGVPDEVKGEVPVAFVVLKNERK
                     IDEIKKEVIDITREKIGPIATPKYIFVVKDLPKTKSGKIMRGLLKKIIMGEDLGDISA
                     LSNPESLKHIQKQINADN"
     gene            2654..3205
                     /locus_tag="P5570_01865"
     CDS             2654..3205
                     /locus_tag="P5570_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HRR39482.1"
                     /translation="MLPEFQVLLLAMAPISELRGAIPVGVAGLNLPFWEVFLISYFGN
                     LIPVVLLLLFLEPVAHFLSKNFLIFQKFFDWLFQRTRKNIAPSIEKHGKKALVIFAAI
                     PLPMTGGWTSSLAAFLFGMPFKVAFPLIAIGVLIAGSIVSVLTLSGVTIEKYFGWETL
                     LIIIALSAIIYFVIKKYHRKKKI"
     gene            3250..4449
                     /locus_tag="P5570_01870"
     CDS             3250..4449
                     /locus_tag="P5570_01870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR39483.1"
                     /translation="MKIAFISPHTFTYPGGVQKHTLSLKKEFEKKGHIVKIIYPREKI
                     HQKRGKDEILLGGSLVMPGNASRTNLSLNVTPLSIKRRLKKEKFDVLHFQNFGAFLPW
                     QVLGAVDQLEKKPLKILTFHAFLDASRIFKDVPFFMDILNDYILPRFDGVIGVSKPVL
                     SQIKYNGFTKIIPNGVDLDFFNPRGEKIEKFCAVPLNNNLCSGPIINILFVGRIEKRK
                     GLIYLVKAFEILKNISSFGGSNKESNFKNIRLIIVGDGDKKEDIERYVKKHHIPDVFF
                     EGRAKEEDLPKYYRTADICCFPSLYGEAFGIVLLEAMASGKPVVAFANQGYKEVLVDK
                     GADFLVQPKDVQGLVQRLKILIKDDKKRKEMGEWGRQEAEKYSWEEIANQTLKFYKEA
                     INYKIVS"
     assembly_gap    4657..4756
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4757..6220
                     /locus_tag="P5570_01875"
     CDS             <4757..6220
                     /locus_tag="P5570_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRR39484.1"
                     /translation="EGAKTARQAIQSPKEGTILDVIEATADSLKEESREESDIVNLFK
                     KAIEKAQEALKATTEKMEVFKKANVVDAGGFGFLIILQSYLEALEGPVFAQATETEER
                     KEDQKKRKFIQTLSYRFEIVALIKKPKISQDKLRQKLEKLGNSLEIVSVGDRMKLHIH
                     TDFPDEVKNIVRSAGTIESLREEDMSREVVGEESIKKVSIGIVTDEIADLTPKIIDHY
                     KIEVVPFKVDWPEGKDLPGDNIYQKMREADKRGITKLPKTSQAPPKVFQEAYKKQFEN
                     GFEKIICITLSSKLSGGYNSALQAKQTLPEEQRDNVFVFDSYNATCGEGLLVLRAIEL
                     VQEQREAYEILQELKDIAPKVYIYAFLADPKWLEWGGRLSRSQANWVRRLQKIGVRPL
                     IGLKDGVVEQIGFRFGSKDTPEAIFQEIEKNSRKARKEGKKIRVVITHCDNLKEAKKL
                     KERLKEIKAEVSFINLTSSVVGVHVGPGALVAGWMAI"
BASE COUNT         2058 a          906 c         1277 g         1882 t
ORIGIN      
        1 ataattattc ctcataaagc aaggggcaaa ttttttctgc gctaagcgca atacagtaat
       61 acaagcgcag tatatatatt atgaaattta taaaaaaagg aaatttatat tatccgccag
      121 aagattttaa aaaacaatca ttggtaaaga gcaaagaaat ttataaaaaa gcagccgagg
      181 atcccatttt gttttgggag aatgctgcca aggaattaga ttggtacaaa aagtgggaaa
      241 aagcatttta ttttcagcca ccttatttgc agtggttttt gggtgggaaa atcaatcttg
      301 ctcaaaatgc tcttgataga catttaaaaa caaataaaga taaggttgct cttatttggg
      361 aaccagaacc aatagaagaa aaacctcaaa tttttaccta caatgatctt tatcaaaaag
      421 tgaatcaatt agccaactct ctaaaaagaa taggaataaa aaagggagat cgagtagcaa
      481 tttatatgcc aatgattcct gaagcaatca tctcaatgct tgcttgtgct cgaattggag
      541 cagttcatat agtgattttt tctgctttct cgcccgacgc tctaaaaatc agattagaaa
      601 tttcaggggc aaaggtttta attactgctg atggttatta cagaagggga aagattattc
      661 ctttaaaaat aaatgctgac gagggggcga aaggaacatt aattgaaaag gtgattgtag
      721 caaaaagatt aggaaataaa attcctttta ataaagaaaa agatctttat tttgatgaac
      781 taataaaaaa tgaatccact gaatgcgagc cagaaatttt ggactcagaa gacccgtttt
      841 ttattttacc ggaaagcgga accacgggcc aatttttgcc tattttgcac actgtgggcg
      901 gttatacggt tcaggcgcat ttaagcggaa aacttatttt tgactttcaa aaagacagtg
      961 ttttgtggtg tacttctgat attggttggg ttactggcca tacctataca gtttatgccc
     1021 ccttattaaa cggcatcagc actgtgattt ttgaaggtgc acctgactgg cccacaccag
     1081 atcgctgggc tcaaattatt gaaaagaaca aagtatcggt tttttacaca gcgcccacag
     1141 ccattcgaat gtttaaaaaa ctagataaaa aagtgcttca acaatatcaa tttaagaatt
     1201 taagggtgtt gggatcagta ggggaaccaa tggatgaagc cacttggctt tggtattttg
     1261 aaaacattgg aaaagaaaga tgccctattg ttgataccta ttggcagacc gaaaccggtg
     1321 gaattgttat ttcatctttg cccggtgttg gaccgtttcg gccaggattt tgcggtttgc
     1381 cattccctgg tattaaagtg gatattttag atagcaaggg aaagccctgt ctagttgggg
     1441 aacgggggaa tttggttgtt ttaccacctt tttcgcctgg gtttttacgg ggaatttata
     1501 atgatgagca gaaatatgtt gaaacttatt ggaatcaata tggcaaagaa atatatttta
     1561 cttctgatgg tgctgtaagg gatgaaaatg gtttgataaa aattacaggc agaatagatg
     1621 atgttatgaa aatttctggt catcgtttgt caacaaatga tttggaatca gtgattttaa
     1681 aacattctga tataattgaa acagcagtga ttggagtacc ggatgaagtg aagggagaag
     1741 tgccggttgc ttttgttgta ttaaaaaatg aaagaaaaat agatgaaata aaaaaagaag
     1801 taattgacat cacaagagaa aaaattggac ccattgctac tccaaaatat atttttgtgg
     1861 taaaagattt acccaaaacc aaatctggaa aaattatgag aggactttta aaaaaaatca
     1921 ttatgggaga agatctgggg gatatatcag ccctgtctaa tccagaaagc ttaaagcaca
     1981 ttcaaaagca aatcaatgct gataattagt taggttaatt aaggattcaa tcatttgctg
     2041 ttgaagtcct ttgacttagt tcagagaatg ctattgtcct ctgatgaaca agccaaaaca
     2101 gaggagaact gttctccttc gccttcttgt cattaccacc cctcaccttt ccgaaggtga
     2161 ggggggggcg atcccgtggc acgcgtctac tgtcatcgcg agcccgctga aagcgggcgt
     2221 ggcgatcccg atgtatggtt gcgggctgcg cccgagattg cttcgtcgct atcgctcctc
     2281 gcaatgacag tgaagggagg agtttgcctt cttttcgaca cttcgctacg ctaccgttta
     2341 acgtgatacg gtaaacgttg gcgttgcgcg tatcgtctta cggcagagaa gtgcgtaaag
     2401 tatacgcttg ccgtttgacg cttatcgcga cgcgctacgc taccgtttaa acgttttact
     2461 gttttctgat ttctgataaa tgatttctat ttaatattgt ttggtattta aaatttagta
     2521 tttatttgga atttggtatt tggtatttgg tgcttgtttg tattttggtg cttgtgtttt
     2581 ggaatttcta ctaaagtaaa ctttatttta ttactttttt ttgataaaat taaaaggcag
     2641 ataaaaatat tttatgctgc ctgaatttca agttttgctt ttagcgatgg ctccaatttc
     2701 agagttaagg ggagcaatac ctgtaggtgt tgcaggactt aacctgcctt tttgggaagt
     2761 ttttttgatc tcatattttg gaaatcttat tccggttgtt ttgttgttgt tgtttttaga
     2821 acctgtggct cattttttat caaaaaattt tttaatattc caaaaatttt ttgattggct
     2881 ttttcagcgg accagaaaaa acattgcacc ttcaattgaa aaacatggta aaaaagctct
     2941 agtaattttt gcagccatcc ccttaccaat gactggtgga tggactagtt cacttgctgc
     3001 ttttcttttc ggtatgccat ttaaggtggc ttttccttta attgctattg gcgtgttgat
     3061 tgcagggagc attgtttctg ttttaacctt aagtggtgtt actattgaaa aatattttgg
     3121 ctgggaaaca ttattaatca ttattgcgct ttcagccata atttattttg tgataaaaaa
     3181 atatcatcgt aaaaagaaga tataatagta taatagaaag tcaaagacaa cgcaggtatc
     3241 aaacctttta tgaaaatcgc ttttatttct cctcatacct ttacttatcc cggcggagtg
     3301 caaaaacaca ccttgtcttt aaaaaaggaa tttgaaaaga aaggccatat tgttaaaatt
     3361 atttatcctc gggaaaagat tcatcaaaag cggggcaaag atgaaattct gttaggagga
     3421 tccttggtaa tgcccggcaa tgcttcgcga acaaacctga gtttgaatgt tactcctctt
     3481 tcaataaaaa gaagattaaa aaaagaaaaa tttgatgtgt tgcactttca aaattttgga
     3541 gcttttttgc catggcaagt tttgggagca gtagatcagc ttgaaaaaaa gccgcttaaa
     3601 attttgacct ttcatgcttt tttggatgct agcaggattt ttaaagacgt gccatttttt
     3661 atggatattt taaatgatta tatcttgcct cgatttgatg gtgttattgg cgtttcaaag
     3721 ccagtgcttt cccaaattaa atataatggt tttacaaaga ttattccaaa tggtgttgac
     3781 cttgattttt ttaatcccag gggagaaaaa attgaaaaat tttgcgcagt tccgcttaat
     3841 aataatctgt gctctggtcc cataatcaac attctttttg tgggacgaat tgaaaaaaga
     3901 aaaggattaa tttatttggt aaaggcattt gaaattttaa aaaacatcag ctcttttggt
     3961 ggctctaaca aagagagcaa ttttaaaaac atcagattga ttattgtagg agatggtgat
     4021 aaaaaagaag atattgaaag atatgtaaaa aaacaccata ttccagatgt gttttttgaa
     4081 ggaagagcaa aagaagaaga tttgccaaaa tattacagaa ccgctgatat ctgctgtttt
     4141 ccatcgcttt atggtgaggc ctttggaatt gttttgctag aagcaatggc ttcaggaaag
     4201 ccagtggttg cttttgctaa tcaaggttat aaagaggtgc tggtggacaa aggagcagat
     4261 tttttggttc agccaaaaga tgttcaggga cttgtgcaaa gattgaaaat tttgataaaa
     4321 gatgataaaa aaagaaaaga aatgggtgag tggggcaggc aagaagcaga aaaatattct
     4381 tgggaagaaa tcgccaatca aaccttgaaa ttttacaaag aagcgataaa ctataaaata
     4441 gtttcttagg aaacatctac gaatagcgaa tcaatacgaa tatacgaatc tttttaaccc
     4501 tcgtattcgt aaattcgtaa cagattcgta attcgttgat atatttatgc aatacatcac
     4561 taaaaaagaa ttacaagaaa tgattttaaa gtcctgtgag aatgttgaaa aaaacaaaga
     4621 gaaaattaat aaaatcaatg tttttcctgt gcctgannnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnngaag gagcaaagac cgccagacaa gccattcaaa gtccaaaaga
     4801 aggtactatt ttggacgtaa ttgaagccac ggctgacagt ttgaaagaag agtcaagaga
     4861 agaaagcgac attgtaaatc tttttaaaaa agccattgaa aaagcgcaag aggcattaaa
     4921 agcaactact gaaaaaatgg aagtatttaa aaaagccaat gtggtagatg ctggcggctt
     4981 cggtttttta atcattcttc aaagttattt agaggcctta gaggggccgg tttttgctca
     5041 ggcaacagaa actgaagaaa gaaaagaaga tcaaaagaaa agaaagttta tacaaacatt
     5101 atcttacaga tttgaaattg tcgctcttat aaaaaaacca aaaatatctc aggacaaatt
     5161 gcgtcaaaaa ttagaaaaat taggcaattc tcttgaaatt gtttcagtag gggaccgaat
     5221 gaaacttcat atccacactg attttcctga cgaagttaaa aatattgtaa gaagtgctgg
     5281 gaccattgaa agtttaagag aggaagatat gtcaagggaa gtagttggag aagaatcaat
     5341 taaaaaagtt tctattggaa ttgtaactga tgagatagct gatttgactc ccaaaattat
     5401 tgatcattat aagattgagg ttgttccttt taaagttgat tggccagaag gaaaagattt
     5461 gcccggtgat aatatttatc aaaaaatgag agaagctgac aaaagaggaa ttacaaaact
     5521 accaaaaacc tctcaagcgc cgccaaaagt ttttcaagaa gcttataaaa aacaatttga
     5581 aaatggattt gaaaaaatta tttgcatcac cctttcctca aaactttccg gtggttataa
     5641 ttcagccttg caggcaaagc aaaccttacc agaagaacaa agagataatg tttttgtttt
     5701 tgattcttat aatgccactt gcggtgaggg acttttagtt ttaagagcaa ttgaattagt
     5761 gcaagagcaa agagaggcct atgaaatttt gcaggaatta aaagatattg cgcccaaagt
     5821 ttatatttat gcttttttgg ctgatccaaa atggcttgaa tggggaggaa ggttaagccg
     5881 ttctcaagct aattgggtaa gacgcttgca aaaaatagga gtaagacctt taattggttt
     5941 gaaagacgga gtggttgaac aaattggttt tcgttttgga tccaaagata cgcccgaagc
     6001 aatttttcag gaaattgaaa aaaattcaag aaaggcgcgt aaagagggaa aaaagattag
     6061 agtagtgatt actcattgtg ataatttaaa agaagcaaaa aagttaaaag aaaggttaaa
     6121 agagattaag gcagaggttt catttattaa tttgactagt tctgttgttg gtgtccatgt
     6181 tgggccagga gcattggttg ctggatggat ggcgatataa aaa
//
LOCUS       DAOTQD010000030         5804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_11720_length_5804_cov_7.036876, whole genome shotgun sequence.
ACCESSION   DAOTQD010000030 DAOTQD010000000
VERSION     DAOTQD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11720_length_5804_cov_7.036876"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..786
                     /locus_tag="P5570_01880"
     CDS             271..786
                     /locus_tag="P5570_01880"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR39485.1"
                     /translation="MLILVCGLPGSGKTRVSKEISKKIKWSVLRTDKIRKELFSKPTY
                     TQSEKDLVYQVMFLIADELLKNNVNVILDAVFSRKDLRDKARALAKKNKTPFHLVEVR
                     CQEEILLSRIDKRSKRKNLSDADRNIYFETKKEFQPIKEKHIIIDNSYGPKKTKEEIE
                     KFLKLLRISKG"
     gene            complement(757..1761)
                     /locus_tag="P5570_01885"
     CDS             complement(757..1761)
                     /locus_tag="P5570_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRR39486.1"
                     /translation="MDISKALLDPAAYPEKVKKIILIQTHSAWVFLTGKHAYKIKKPV
                     NLGFLDFSTLEKRKFYCNQELKLNRRLAWDLYLGVLPITKEKDGKIKINGKGKIIDFA
                     VVMKEIPQERLMSTLLQKNKVKTKTVKEIAKIIAKFHQKKYPRVKKEYKSEKIIKRNW
                     NENFEQTKPFVEKTISKKDFDLIEKAIKNFFKKNNTLLQKRVRENKIKWCHGDLHSGN
                     IFITEKNKIYIFDCIEFNERFACQDVANEVAFLAMDLDFWNKKDLADCFIKSYLEYTK
                     DQELLKLLNFYKCYRAFVRGKVISLKTCDKNIEKNEQKKAAREAKKYFKLSLAYAKQF
                     "
     gene            2301..3551
                     /locus_tag="P5570_01890"
     CDS             2301..3551
                     /locus_tag="P5570_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010978302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRR39487.1"
                     /translation="MSKIKDIKLSKNGSLQIEWAETHMPVLMQIRDRFLKNKPFKNFR
                     IACCLHITKETGVLMKTLRAGGATVALAGSNPLSTQDDIAAALVKEGVEVFAWRGIGK
                     AQYYQCINKVLDTKPNLTMDDGGDLVSVLHSKRQNLIKNVVGGTEETTTGVIRFRAME
                     RDKVLRYPIVAVNDAQTKYLFDNRYGTGQSTIDGILRTTSILLAGKIFVVCGYGWCGR
                     GIAMRASGMGSKVFVCEVDPVKALEAKMDGFEVLPMIEAAKIGDIFVTATGDKDVIRK
                     MHFLKMKSGAILANAGHFNVEINVDELKALSQKRRVIRDNVEEYQLKNGKKIYLLAEG
                     RLINLAGAEGHPSEVMDMSFANQALVAQWLREKEGSLEPRVYKVPEKIDNEIATLKLR
                     ALGAKIDKLTPEQKKYLASWQEGT"
     gene            3724..4272
                     /locus_tag="P5570_01895"
     CDS             3724..4272
                     /locus_tag="P5570_01895"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRR39488.1"
                     /translation="MKKEEEIKKLNEEIEKCKKCLLAKTRKNTVPGEGPVSAKIMIIG
                     QAPGAQEDKTGKPFVGRAGKLLNQLLAISKIERKKVFITSPIKCFPPKNRKPTKKEIE
                     ICFPWLKKQIEIINPSKIILLGGVAFSIFFPKKDLKDFRGKWMKLDNKEFFITYHPAA
                     GLRFPRIKKILEKDFQLLTFKK"
     gene            4368..5726
                     /locus_tag="P5570_01900"
     CDS             4368..5726
                     /locus_tag="P5570_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRR39489.1"
                     /translation="MFSTNIFKAYDIRGVYKQDFEAKDAIKIGQAFAEFLRTNKRKPL
                     TVIVGRDNRLSSPALTKAIKEGIKLQGVNIIDIGLSTTPMFYFGVNFLKADGGIMVTA
                     SHNPPEYNGFKMVREEAIPISEDSGIKEIKKMVLGGKFTKRKPGTEIKKNITNAYIKF
                     NLDFLDQSKIAPLKIAVDTANASSGVLIPKIAEKIKKTKFYHLFSELDGRFPNHNPDP
                     LTKENLIHLQKEVLKKKADLGVAFDGDGDRTVFVDEKGKAISGDLITALIAKIILREK
                     PGEKILYDVRSSKTVGEVIKENNGKPVFCRVGHSFIKEKMRQEDIIFGGELSGHYYLR
                     ENHFCETPFFVLFKIMEEISQEKKPFSKIIKPLQRYAYSGEINFEVKDKDKTMKLIEK
                     HFKGKKCKVSHLDGTKMDFGDWWFLVRPSNTEPLLRLIVETKDKKTMKEKIKKIEGII
                     KK"
BASE COUNT         2006 a          868 c         1067 g         1863 t
ORIGIN      
        1 ataattttga tttttttgta tttgacacga gcgtaaaaat tccaaatcac aaatttcaaa
       61 taacaaacag aaaacagtaa aacgtttaaa cggtagcgta acgcgtcgcg gcaagcgtaa
      121 aacggcaagc gtatacttac gcacttctct accgtaagac gatacgcgca acgccaacgt
      181 ttatcgtatc acgttaaagt aatattgcat taaatttttg aacctaacta attatatttt
      241 tactgctaat ttaaattttt aattccatat atgctaattt tggtttgtgg cttgccaggt
      301 tctggtaaga cgagagtatc aaaagagatt tcaaaaaaaa taaaatggtc tgttttgcgg
      361 actgataaaa tcagaaaaga attgttttca aaaccaacct atactcaatc tgaaaaagat
      421 cttgtttatc aggttatgtt tttgatcgct gatgaacttt taaaaaacaa tgttaatgtg
      481 attttggatg cagtattttc aagaaaggac ttgcgagata aagcaagggc actggcaaag
      541 aaaaataaaa caccttttca tttggttgaa gtaaggtgtc aggaagaaat attgctttca
      601 agaatagata aaagatcaaa aagaaagaat ttaagcgacg ctgataggaa tatttatttt
      661 gaaacaaaaa aagaatttca gccaataaaa gaaaaacata ttattattga taattcttac
      721 ggtcctaaaa aaacaaaaga agagatagaa aaatttttaa aattgcttcg cataagcaag
      781 ggataattta aaatattttt ttgcttctct tgctgctttt ttttgctcat ttttttcaat
      841 atttttgtca caagttttta aagaaattac cttccccctg acaaaagccc gataacactt
      901 ataaaaatta agcaatttta ataattcttg atctttggtg tattccaaat aggatttaat
      961 aaaacaatca gcaagatctt ttttgttcca aaaatctaaa tccattgcta aaaaagctac
     1021 ctcattggcg acatcttgac aggcaaatct ttcattgaat tcaatacaat caaagatgta
     1081 aattttattt ttttcagtaa taaaaatatt gccggaatga agatcgccat ggcaccactt
     1141 aatcttgttt tcccttactc ttttttgcaa aagggtatta ttttttttaa aaaagttttt
     1201 tattgctttt tcaattaaat caaaatcttt ttttgaaata gttttttcaa caaacggctt
     1261 ggtttgttca aagttttcat tccagttacg ctttataatt ttttcgcttt tgtattcttt
     1321 ttttactcgg ggatattttt tttgatgaaa cttagcaata attttagcta tttccttgac
     1381 tgtttttgtc tttactttgt ttttttgtaa caaagtgctc attaatcgtt cctggggaat
     1441 ttctttcatt actactgcaa aatcaataat cttgcctttg ccattgattt taatctttcc
     1501 gtctttttct tttgtgatgg gcaagacccc aagatataaa tcccaagcaa gacggcggtt
     1561 tagtttcaac tcttgattgc agtaaaactt tcttttttcg agagtggaaa aatctaaaaa
     1621 ccccaggttt acaggctttt ttattttata agcatgcttg cctgttaaaa acacccaggc
     1681 gctgtgggtt tgaataagga taattttttt tactttttct gggtatgcag cgggatccaa
     1741 aagggccttg cttatatcca taaaatattt tatacttaat ttagcctata tgtctttttg
     1801 caagagtagg gaataaccta ttgcaaatat ttatattaaa tttataatct acttaggttc
     1861 aagaattcaa cgcaacacca caaatgtcat cgcgaggagc cgcagagaag caaccccata
     1921 tctgtcattg cgaggagtct cgcctttggc gagatgacga agcaatctcg agcgagtgaa
     1981 gcgagctcgc aacaatactc tgggattgcc acgttcggga gtttcactcc ctcgctcgca
     2041 atgacaatgt ttattcgtta tctttgacgc gagccgcagc gaagcgttga aaagaataca
     2101 ttgtcattgc gagcggtagc gaagcaatct cgagcaaccg aagggagctc gttgaaaaga
     2161 agatgaaatc ttctttgagt tcggaccgca gtccgcaact atacttcgag attgcttcgt
     2221 cggcctataa ggccttctcg caaagacaag tattgttgcg ttaaaaactt gattctaccc
     2281 tactataatt atacaaaaat atgtcaaaaa taaaagatat caaattatca aaaaatggaa
     2341 gtttacaaat tgaatgggca gaaactcata tgccggttct aatgcaaatt cgagatcggt
     2401 ttttaaaaaa caagcctttt aaaaatttta gaattgcttg ttgtctgcat attacaaagg
     2461 aaacaggtgt tttgatgaaa acattaagag ccggtggagc aacggttgct ttagctggtt
     2521 ccaatccatt atctactcaa gacgatattg cagctgcttt ggtaaaagaa ggtgttgaag
     2581 tgtttgcctg gcgagggata ggcaaagctc aatattatca atgcattaat aaagtgcttg
     2641 atacaaaacc aaatcttaca atggatgacg gtggagatct tgtaagcgtg cttcattcca
     2701 agcgtcaaaa tcttatcaaa aatgtggttg gtggtacaga agaaacaact actggcgtta
     2761 taagatttag ggcaatggag agagataagg ttttgcgcta tccgatagtg gcagttaatg
     2821 atgctcaaac caaatattta tttgacaata ggtacggtac tggccagtct acaattgatg
     2881 gaattttaag gactacttct attttgcttg ctggaaaaat ttttgtggtg tgtggttatg
     2941 gatggtgtgg taggggaatt gcaatgcgtg caagtggaat gggatctaag gtgtttgtat
     3001 gtgaggttga tcctgttaaa gccctggagg caaaaatgga cggttttgaa gttttaccca
     3061 tgatagaagc agccaagatc ggagatattt ttgttactgc tactggcgat aaagatgtta
     3121 ttagaaagat gcacttttta aaaatgaaat caggcgctat tttggcaaat gctgggcatt
     3181 ttaatgttga aattaatgtt gatgaactta aagcattatc tcaaaagaga agggttatta
     3241 gagacaatgt tgaagaatat caattaaaaa atgggaaaaa gatttattta ttggctgagg
     3301 gacgattgat taatttagct ggtgctgagg ggcacccctc agaagtaatg gatatgagct
     3361 ttgctaatca agcattagtt gctcaatggc tgagagagaa agaaggaagt ctagagccca
     3421 gggtttataa ggtgccagag aaaattgata atgaaatcgc tactttaaaa ttaagggcct
     3481 tgggggcaaa aattgataaa ttaacgcctg aacaaaaaaa ataccttgct agttggcaag
     3541 agggcacata aaaatacttt aacgttttaa ttctttttca tctttttcag attaaaaggg
     3601 gaatacaaaa taaattgttg ccccttttta taatttttta ttaatttttt ttaattttcc
     3661 ttctttgtgc tttttcgtca attttatcac taattatatt ttagattgtt ttataaaatt
     3721 tttatgaaaa aagaagaaga gattaaaaaa ttaaatgaag aaattgaaaa atgtaaaaaa
     3781 tgtcttctag caaagaccag gaaaaatact gttcccggcg agggaccggt tagtgctaaa
     3841 attatgatta taggccaggc tccaggtgcc caggaagata aaaccggaaa gccgtttgtg
     3901 gggagagctg gaaaactttt aaatcaactt ttagcaataa gcaagataga aagaaaaaaa
     3961 gtttttatca cttctccaat taaatgtttt ccacccaaaa atcggaaacc gacaaaaaaa
     4021 gaaattgaaa tctgtttccc ctggcttaaa aaacaaattg aaattattaa tccttcaaaa
     4081 attattttat taggcggggt agctttttca atttttttcc caaaaaagga tttgaaagat
     4141 tttcgaggaa aatggatgaa actggacaat aaagaatttt ttattaccta tcatccagca
     4201 gccggtcttc gtttccccag aattaaaaaa attttagaaa aagattttca attgttgact
     4261 tttaaaaagt aaaaaagtaa aatatttctt acgaagttag tgaggcgttt tattctaatc
     4321 agtcattaaa aaggcttgat tagattaatt aattagtaat taaatttatg ttttcaacaa
     4381 atatttttaa agcctacgat attcgaggtg tttataaaca agattttgaa gcaaaagacg
     4441 ccattaaaat tggtcaagct tttgctgaat ttttaagaac aaataaaagg aagcccttaa
     4501 cagttattgt tggtcgcgat aatcgacttt cttctcctgc tttgaccaaa gcaataaaag
     4561 aaggtataaa acttcagggc gttaatatta ttgatattgg tctttccact acccctatgt
     4621 tttattttgg ggtcaatttt ttaaaggcag atggtggaat aatggtaact gcttctcaca
     4681 acccaccaga atacaatgga tttaaaatgg tgagagaaga agccattcct atttctgaag
     4741 acagtggtat taaagaaatt aagaaaatgg ttcttggcgg caagtttaca aaacgaaagc
     4801 caggcactga aataaaaaag aacataacca acgcttatat taaatttaac ttggattttc
     4861 ttgatcagtc aaaaattgca cctttaaaga ttgctgttga tactgccaac gcttcttctg
     4921 gagttttaat tccaaaaatc gcggaaaaaa ttaaaaagac aaaattctat catttatttt
     4981 cagaattaga cgggcgtttc cccaatcata atcccgatcc ccttacaaaa gaaaatttaa
     5041 ttcatctcca aaaagaagtt ttaaaaaaga aagctgatct tggagtggct tttgatggtg
     5101 atggcgacag aacagttttt gttgacgaaa agggtaaagc aatctctggt gatttaatta
     5161 ctgcccttat agcaaaaatt attttaagag aaaaaccagg tgagaaaatt ttatacgatg
     5221 ttcgttcaag caaaacagtg ggagaagtga ttaaagaaaa caatggtaaa ccggtatttt
     5281 gtcgcgttgg tcattcattc atcaaagaaa aaatgcgtca agaagatatt atatttggag
     5341 gggaactttc tggtcattat tatttaagag agaatcattt ttgtgaaacg ccgttttttg
     5401 ttttgtttaa aataatggaa gaaatatctc aagaaaaaaa gcccttttca aaaattatta
     5461 agccccttca aagatatgct tattctggtg agattaattt tgaggtaaag gacaaagaca
     5521 aaacgatgaa attaattgag aaacatttta aagggaaaaa atgtaaagtt tctcatcttg
     5581 acggcacaaa aatggatttt ggcgattggt ggtttttggt ccgtccatca aatactgaac
     5641 ctcttttgcg attaattgtt gaaacaaaag acaaaaaaac catgaaagaa aagataaaga
     5701 agatcgaagg aataattaaa aaataatttt tacagtgttg tcattgccca aacagaaatc
     5761 agttgtcaga aatcagaaaa cagaaattat tattacataa attt
//
LOCUS       DAOTQD010000031         5397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_12750_length_5397_cov_8.556159, whole genome shotgun sequence.
ACCESSION   DAOTQD010000031 DAOTQD010000000
VERSION     DAOTQD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12750_length_5397_cov_8.556159"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1182)
                     /gene="dnaJ"
                     /locus_tag="P5570_01905"
     CDS             complement(76..1182)
                     /gene="dnaJ"
                     /locus_tag="P5570_01905"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRR39490.1"
                     /translation="MKDYYKILGVSEDASQEEIKKAFYRLAHKYHPDKGGSAEKFKEI
                     NEAYQILGNKDKRAKYDASKKAGFDFEGSPFGSGSQGSPFGSGFGFGRGFEDFEGFEG
                     FEDLGDIFEEFFSGGPFGGTRQKNKRGKDIMVEVEMTLEEVFTGVQKNLSLAKFIVCP
                     ACNGTGAEPGAKMETCPSCRGTGQVHQVHQTFLGSFTKSGTCPQCNGTGEFPEKKCKK
                     CAGEGRVKDIETISFFIPAGVQNNEVIKIEGKGEAGAKGEKSGDLLVKIHVKDHPYFQ
                     RRGDDIYYDLTVNFSQAALGDKVDVPTLAGQVELKIPSGTQQGKLLRLKGMGIPHLNG
                     RGKGDMYVKIHIKTPTRLTRKQKELIEKLREEGI"
     gene            complement(1266..3161)
                     /gene="dnaK"
                     /locus_tag="P5570_01910"
     CDS             complement(1266..3161)
                     /gene="dnaK"
                     /locus_tag="P5570_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRR39491.1"
                     /translation="MAKILGIDLGTTFSAMAVVEAGEPKILENAEGERTTPSVAAISK
                     TGERLIGTLARRQAITNPKNTIYSVKRLIGRRFSDPEVQRDKKLLSYEIREGSDGGVE
                     VKMGDKWYKPQEISAMILQKLKTDAEAKLGEKINDVIITCPAYFDDSQRKATKDAGEI
                     AGFNVRRVINEPTAAALAYGLQKKKDQQIVVYDFGGGTFDISVLNVTSDTIEVKATGG
                     DTHLGGDDFDQRIMDYVVENFKKDEGIDLSKDPLALQRIKEAAEKTKRELSVTMQAEI
                     NLPFITSDASGAKHLSYTITRSKLEELVSDYIERSIELVKQTVKEAGFELSDIDEVVL
                     VGGQTRMPKIQQEVKKLFGKEPNKEINPDEVVAIGAAVQGGIMQGEMKEVLLLDVTPL
                     SLGIETLGGVNTVLIPKNTTIPTSKSQIFSTAADNQTSVEIHVLQGERPMAKDCKSLG
                     RFILDGIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKSQSIRIEAQSGLSKEEVE
                     RLKKEAEIYAEEDQKKKDLIEAKNVAETLVYTSQKTLADAGDKISQEIKKEVEEKIQD
                     LQKVLNGEDINEIKQKTQELSQSIQKIGAQMYGQGQQGEGKEKKNDNENGDDKKGNAD
                     EGEFKKK"
     gene            complement(3415..3831)
                     /locus_tag="P5570_01915"
     CDS             complement(3415..3831)
                     /locus_tag="P5570_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR39492.1"
                     /translation="MAIIPWEPFRDLDKFFEDEDFFPVIPMRRTDFPAVDIAEDEKNV
                     YVEMPMAGVKPSDVEIAVEDEVLTVKGKTEEEKEEKKKNYWRKEIRKGEFERSVLLPS
                     EVKAQQAKAEFKNGMLKVTLPKKEVKKSQKVQVKVK"
     gene            complement(3897..4457)
                     /locus_tag="P5570_01920"
     CDS             complement(3897..4457)
                     /locus_tag="P5570_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRR39493.1"
                     /translation="MAEKKEKEEKENASQNIEYIEEESEKSFDVEKKIKRLKEELKHC
                     QKEKEEYLAGWQRAKADFINYKKEEQIRIKEGVLFEKKKILLEIISLLDNLERAEENL
                     SDDLKGNVWVEGILKIKDQIKSFLEKEGVKEIKVEKDFNPVFCEAVQMGDGADGEVIE
                     VLQKGYLFNDSVLRPAKVKVGKGREN"
     gene            complement(4589..5266)
                     /locus_tag="P5570_01925"
     CDS             complement(4589..5266)
                     /locus_tag="P5570_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39494.1"
                     /translation="MEKRQEKVLKCLVKEYIKSAKPVSSQMLFEKYHLGISPATIRWE
                     MVELTDSGYLYQPYASAGRVPTEKAYRFFIEKFCEPRLSPTLEQKIEEVFQGRDEREI
                     LRALGRLMSLVSRNISILFFEGEVFWQGLSYLLSQPEFDDPKESLRVIKVFEELYQDI
                     YKNDDKEKEIKVYIGRENPFGKDENLSLIMGGLEDGLVGILGPTRMDYQKNIALVEKV
                     REILEEM"
BASE COUNT         1501 a         1226 c          699 g         1971 t
ORIGIN      
        1 aaaatttaat cttacaaaag tttagaaatt aaaaattaga aattcttaat ttttgacaag
       61 atgtcaaaaa ttaagctata ttccttcttc tcttaatttt tctattaatt ctttttgttt
      121 tctggttaat cttgttgggg tttttatatg aattttcacg tacatatcac ctttcccccg
      181 tccattcaaa tgaggaatgc ccattccctt taatcttaat agttttcctt gctgagtgcc
      241 agatggaatc tttaattcta cctgaccagc caatgtggga acatctacct tgtctcccaa
      301 agctgcttga gaaaagttca ctgttaaatc atagtatata tcatctccac gtctttgaaa
      361 ataaggatga tctttaacat gaatcttaac tagaagatct cctgattttt ctccttttgc
      421 acctgcttct cccttgcctt caattttaat cacctcgtta ttttgtacac cagctggaat
      481 gaaaaaagag atggtttcaa tgtcttttac tctgccttct ccagcacatt ttttacattt
      541 tttttcagga aactctcctg taccattaca ttgaggacaa gtaccgcttt ttgtaaaact
      601 gcctaaaaaa gtttgatgaa cctgatgcac ttggcctgtg cctcgacaag aaggacacgt
      661 ttccattttg gcaccaggtt cagccccagt accattgcaa gcaggacaaa ctataaattt
      721 agcaagagag agattttttt ggacaccagt aaaaacctct tccaaagtca tctctacctc
      781 gaccataatg tctttgcccc ttttattttt ctgacgtgtg ccaccaaaag gcccgccact
      841 aaagaattct tcaaaaatat ctcccaaatc ctcaaaaccc tcaaagccct caaaatcctc
      901 aaatcctcta ccaaaaccaa atccactacc aaatggactg ccctggctac cgcttccaaa
      961 tggactgccc tcaaaatcaa aacctgcttt ttttgatgca tcatatttgg ctctcttatc
     1021 tttgtttcct aatatctgat atgcctcatt tatttctttg aatttttctg cagaaccacc
     1081 cttatcagga tgatatttat gagccaaacg ataaaacgcc ttcttaattt cctcctgaga
     1141 agcatcttct gacactccca agattttgta ataatctttc ataaaattat cagcgaatta
     1201 aaaactatat ttatcaatcc ctgataaaat acatcttcat caattcaaaa ctcgttgttt
     1261 ttatactact tctttttaaa ttctccttca tctgcgtttc cttttttatc atcgccattt
     1321 tcattatcat tctttttttc ttttccctcg ccctgctgtc cctgaccata catttgagct
     1381 cctatttttt gaatcgattg agaaagttct tgagtctttt gctttatttc atttatatct
     1441 tctccattca ggaccttttg aagatcttga atcttttctt caacttcttt ttttatttcc
     1501 tgagaaatct tatcacccgc atcagctaag gttttttgag aagtataaac cagtgtctca
     1561 gctacattct tggcctcgat aagatccttt ttcttttgat cttcctctgc gtaaatttca
     1621 gcttcttttt taaggcgttc tacctcttcc tttgaaaggc cagattgagc ctctatcctg
     1681 atcgattgtg attttccagt ggctttgtct ttagcagaaa catttaaaat gccattagca
     1741 tcaatatcaa aagtaacttc aatctggggc acaccacgtg gagctggagg aataccgtct
     1801 aaaataaatc tccccaaact cttgcagtcc ttggccattg gcctctcgcc ctgcaacaca
     1861 tgaatttcta ccgaggtttg attgtcagca gctgttgaaa aaatctgact ttttgatgtt
     1921 ggaatggttg tgttctttgg gatcaaaaca gtatttactc ctcccaaggt ttcaataccc
     1981 agcgataatg gtgttacatc taataacaac acttctttca tttctccctg cataatacct
     2041 ccctgcactg ctgcaccaat tgcaactacc tcatcggggt taatttcttt attaggttct
     2101 ttgccaaata attttttcac ttcttgttga atttttggca ttcttgtttg ccctcctacc
     2161 aaaactactt catcgatatc agaaagctca aatcctgctt cttttacggt ctgttttaca
     2221 agctcaattg atctttcaat ataatctgat accagttctt ctagtttgga acgagtaata
     2281 gtataagaca agtgctttgc gcctgaagca tctgaagtga taaatggtag attaatttcg
     2341 gcttgcattg taactgataa ttctcttttt gttttttctg ctgcttcttt tatacgttgc
     2401 agtgctaaag gatcttttga aagatcaatt ccttcgtctt ttttaaaatt ttcaacaaca
     2461 taatccataa tccgctgatc aaaatcatct cctcccaaat gagtatcacc tcctgttgcc
     2521 tttacctcaa tagtatcaga agtaacattt aaaactgaaa tatcaaaagt tccgccacca
     2581 aaatcataga caacaatttg ctgatccttc tttttttgaa gaccgtaagc caaagccgca
     2641 gcagtcggct cattaatcac tctccttaca ttaaaaccag caatctcacc tgcatccttt
     2701 gtggcttttc gctgactatc atcaaaataa gcaggacaag taataataac atcattgatt
     2761 ttttctccta attttgcctc ggcatctgtt tttaattttt gaagaatcat tgccgagatt
     2821 tcctggggtt tataccattt atcacccatt ttcacttcta ctcctccgtc tgaaccttct
     2881 cttatttcat aagaaagcaa ttttttatct ctttgaactt cgggatcaga aaaccgacga
     2941 ccaataagtc gcttaacgct ataaattgta tttttggggt tagtaatggc ttggcgacga
     3001 gccagggtgc caatcaatct ttctcctgtt tttgaaatag cagcaaccga gggagtagtt
     3061 ctttctccct cagcgttctc gagaattttt ggctcaccgg cttcaaccac agccattgct
     3121 gaaaacgttg tacccaaatc aatgcctaaa atttttgcca taagtttaaa aataataatt
     3181 ctaatattat ttgaatttat tcaaataatg taaatatata taaacctgta ttacatatcc
     3241 aaatgagatc cgaataattc aaattactac aaatttatat cacctaaagt gcttaacggt
     3301 agcgtcacgc gtatcgtctt acagcagata aatgcgtaaa acttaaggta tgtgtttgac
     3361 ttttgctgat acgcgtgacg caaacatcta ccgtatcagc tctgtttaaa tattttattt
     3421 gactttgacc tgaacttttt gggacttctt cacctctttt ttgggcaatg ttactttcaa
     3481 catcccgttc ttgaactcag cttttgcctg ttgtgccttt acttcgcttg gtaaaagaac
     3541 agatctttca aactcacctt tcctaatttc tttcctccag taatttttct ttttctcttc
     3601 tttttcttct tcggtttttc cctttacagt taaaacctca tcttccactg caatttccac
     3661 atcagatggc tttaccccgg ccattggcat ttcaacatat acattctttt catcctcagc
     3721 aatatcaaca gctggaaaat cagtccttct cattggaatt actgggaaga agtcctcatc
     3781 ttcaaaaaac ttatccaggt ctcgaaatgg ttcccaaggt ataatagcca tatgttttaa
     3841 ctttagtttc ttaggaatta attttaaaat tgcctaagaa actaaaaaac tataaattaa
     3901 ttttctcgac ctttgcccac ttttacctta gcgggcctta aaacactatc attaaaaaga
     3961 taaccttttt gtaaaacctc tattacctca ccatcagcac catcacccat ttgaactgct
     4021 tcacaaaaca caggattaaa atccttttct acctttattt ctttaactcc ctccttttct
     4081 aaaaaggatt ttatctggtc tttaattttt aatattcctt ccacccaaac atttcccttt
     4141 aaatcgtcag ataaattttc ttctgccctt tcaaggttgt ctaatagtga aattatctca
     4201 agcaagattt ttttcttttc gaataacact ccttctttta ttcgtatctg ctcttctttc
     4261 ttatagttaa taaaatctgc ttttgccctt tgccatccag ctaaatattc ttctttttcc
     4321 ttctgacaat gtttcaattc ttctttcaat ctttttatct ttttctcaac atcaaaactt
     4381 ttttcacttt cttcctctat atattctatg ttttgggatg cattttcttt ttcttctttt
     4441 tctttttttt ctgccataat gttctatgaa attactttgt tgagctcgtc agaacgagca
     4501 caagataaat tctctgtaaa taactcaaca aaatctttaa atcttaattg caaaacctta
     4561 acaacaaaat atcatttttt atcgaaattc acatttcttc taatatctct cttacttttt
     4621 ccactaacgc aatattcttt tgataatcca ttctggtcgg ccctaaaatt cctaccagcc
     4681 catcttctaa gcctcccatg attaaactca aattctcatc ttttccaaag gggttttctc
     4741 tgccaatata aaccttgatt tctttttctt tatcatcatt tttgtaaata tcctgataca
     4801 attcttcaaa caccttgatc actcttaaac tctcttttgg gtcgtcaaac tccggctgag
     4861 ataaaagata agataatccc tgccaaaaaa cctctccttc gaaaaagaga atactgatat
     4921 ttcttgaaac caatgacatc agtctaccca atgccctcag tatttccctt tcatctctcc
     4981 cttgaaaaac ctcttctatc ttttgttcca atgttggtga tagccgcggt tcacaaaact
     5041 tttcaataaa gaaccggtag gccttctctg taggcactct gcctgcagag gcatagggtt
     5101 gataaagata accgctatct gttaattcta ccatttccca tcgaatagta gccggactaa
     5161 tacccaaatg atatttttca aacagcattt gtgaactcac tggctttgca cttttaatat
     5221 attccttcac caaacacttt aatacttttt cttgtctctt ttccatatac ttattttata
     5281 ccaaaaattt ttagcagtca aggggtctga ctgctaatag aacaaaaata atagaaattg
     5341 aatatattcg ctatacgata tgtacctttt tagacgatcg tctaaaaagg tacattg
//
LOCUS       DAOTQD010000032         5364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_12841_length_5364_cov_8.268789, whole genome shotgun sequence.
ACCESSION   DAOTQD010000032 DAOTQD010000000
VERSION     DAOTQD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12841_length_5364_cov_8.268789"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..318)
                     /locus_tag="P5570_01930"
     CDS             complement(121..318)
                     /locus_tag="P5570_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39495.1"
                     /translation="MKSSTVAIIIIVLVAIGIIVWAVTSSQEGTPVEEETQVEEGTPV
                     EEETPVEEETPVEEGTEETEE"
     gene            complement(455..589)
                     /locus_tag="P5570_01935"
     CDS             complement(455..589)
                     /locus_tag="P5570_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39496.1"
                     /translation="MTQFQDQSADEEIVRRIQSGEIELFDILFERYEKNFTLCQNLKS
                     "
     gene            complement(608..1321)
                     /locus_tag="P5570_01940"
     CDS             complement(608..1321)
                     /locus_tag="P5570_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR39497.1"
                     /translation="MQRLLILHGWGSNAGRWQKVKEILEKEGIEVLIPNLPGFGFTPP
                     PKEPWGIDDYVNWVLDFLKEKEWTRFNLLGHSFGGGLATRIAETKPEMVEKLILCAAA
                     VIRDEKSKKTLFFYSLGKVGKKIFSLPGLRKFYPAAANALYTLNNSKDYYLAEGVMKD
                     TFKRVTSQDLKISLEKITAPTLILWGSKDDMVPEKDAHILHEKIKNSKLVIFPGIGHN
                     LNTQIPEKIANETLKFLKK"
     gene            complement(1446..1519)
                     /locus_tag="P5570_01945"
     tRNA            complement(1446..1519)
                     /locus_tag="P5570_01945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1483..1485),aa:Arg,seq:ccg)
     gene            complement(1531..2247)
                     /locus_tag="P5570_01950"
     CDS             complement(1531..2247)
                     /locus_tag="P5570_01950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR39498.1"
                     /translation="MDIEYANFLLKKVHQDYNTMSGQFARARGKAWEEMRFLFDDYVK
                     GKERILDLGCGNGRFYEMLEGKDIEYIGVDVSKELIDIAKNKFPGVDFRVGDAINLPF
                     IDEYFDKVYSIALLHHIPSKEIRLRVLAEAKRVLKKNGLLILTVWNLWEKKFTRDLIF
                     QQTKAKVLGKSKLDFKDILMKWEGVDDCYFHCFTERELKKLVKRAGFSIIKSGEILVG
                     TKSKKTKKLPTSNLFVVAQK"
     gene            2240..4051
                     /locus_tag="P5570_01955"
     CDS             2240..4051
                     /locus_tag="P5570_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HRR39499.1"
                     /translation="MSIDPNEIIIKNLIKINAKNQSDLAQTKREIAKHYKVPCPSNIK
                     LLEVYHNLLYTDKIEKNENLENLLIKRKIRSLSGVVVVSVLTKPYPCPGKCIYCPKEK
                     GIPKSYLSGEPAVERAKKLNFDPYLQVKKRLEMLQNEGHPTDKIELRIIGGSWSCYPK
                     DYKQWFITKCFEAANKFNKNSKFQIPNFKQVSIAEPCKSGQDARRDEFSLNSKQGVKR
                     GEASLNSKQGVKRDEASLNSKQGAKRDEASLSSNIQNLKQEQKRNETAKHRIVGISIE
                     TRPDLINEKEIKELRKLGVTLVEIGVQTIFDDVLKKCQRDHSVKEVIEATKLLKDAGF
                     KVLYQMMPNLPGSTIQKDLATFKELFENPHFKPDWLKIYPCVVCKESKLYQLWEKGKY
                     QSYSDEELINLLIKIKEILPYWVRVARLFRDIPSSKIQSGSKVSNLREVVLNKLNQDG
                     KSCHCIRCREVRENYNPKEKIYLKREDYDASGGKEIFLSFENKNRTKLFAFLRLRIPS
                     FVFSREKHFLPVLQDAAIIREIHTYGALTPFNKKTNLSPQHQGMGRALIKEAEYIVKN
                     EFRLPKIAVISGVGVRNYWRKLGYRLNNTYMVKNQIL"
     gene            complement(4069..4725)
                     /locus_tag="P5570_01960"
     CDS             complement(4069..4725)
                     /locus_tag="P5570_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39500.1"
                     /translation="MPADINIFWKSIEQFGPLLRVLIAVLIVLVGWLVSGGIGQLVTA
                     LLNKFKLNQILKRMGWEESLARAEIRLDAPKFFGEIVKWCFVIIFLMVACEVINLPQF
                     SEFLGQVIAYLPNLLVAALIFVVAVFLADFSYRIVVASSEKAKISYSKLLGSGIRWAI
                     WVFAILAILLQLGITPDIVRALIYGMIAMITIATGLAFGLGGKDLAAEILKELKDKLS
                     "
     gene            5266..5337
                     /locus_tag="P5570_01965"
     tRNA            5266..5337
                     /locus_tag="P5570_01965"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5298..5300,aa:Met,seq:cat)
BASE COUNT         1868 a          982 c          756 g         1758 t
ORIGIN      
        1 ttgaatcttc ccaagattaa atttaattta actcatttta aaatttttat caattttgat
       61 ttttaaaaat taaagcccga gatcacttct cgggctttaa ttataagaat tataattcct
      121 ttactcttct gtttcctcag ttccttcttc aactggggtc tcttcttcaa ctggagtttc
      181 ctcctctact ggagttcctt cttcaacttg agtttcttct tcaactggag ttccttcttg
      241 actgctagta acagcccaaa caataattcc aatcgctact aaaactataa ttataattgc
      301 tacggtacta gatttcatag gtatattgtt ttttttatta tttaattcga ccttttttct
      361 acttggcaaa atataatcca gccctcttat atcgccaagt atgatattta tattttatca
      421 taaattgatc ttattttgca aattttactg atatttaact ttttaaattt tggcatagcg
      481 tgaaattttt ttcatatcgt tcaaaaagta tatcaaaaag ttcaatctct ccagattgaa
      541 ttcgtcgcac aatttcttcg tctgccgatt gatcctgaaa ttgagtcata taaaatacta
      601 aattgattta ttttttcaaa aattttaatg tttcgttggc tattttttca ggaatttgag
      661 tgtttaaatt atgaccaatt ccaggaaata ttactaattt tgagttttta attttctcgt
      721 gcaaaatatg agcatctttt tcaggaacca tatcatcttt gctaccccac aaaatcaaag
      781 ttggagctgt tattttttct aaactaattt ttaaatcctg actcgtaact cttttaaagg
      841 tatctttcat taccccttct gctaaataat aatcttttga attattaagt gtgtataagg
      901 cattggcagc agcaggataa aacttacgca atccaggcaa agaaaaaatc ttcttgccca
      961 cttttcctaa ggaataaaaa aataatgttt tttttgattt ttcatctctg ataacagcag
     1021 ctgcgcataa aatcaatttt tcaaccattt ctggttttgt ttctgctatc cttgtggcta
     1081 atcctcctcc aaaagaatgc cctagtaaat taaatctcgt ccattccttt tcctttaaaa
     1141 aatctaacac ccaatttaca taatcatcaa tcccccaggg ttcttttggt ggcggtgtaa
     1201 agccaaagcc aggtagattg ggaattaaaa cctctatccc ctctttttct aaaatttctt
     1261 tcaccttttg ccaacgacca gcatttgatc cccagccatg taaaataagt aaacgttgca
     1321 taataatgat gataatttat cgcaaaggtt tatgtctatc gttgtctact ctgaaaggtt
     1381 ataaacaagg atcacaacga ttgaccagac caattactat aaatctgata gataaaaaaa
     1441 tattatgtcc ccggtgcgat ttgaacgcac aatctttagc tccggaggcc aacgctctat
     1501 ccagttgagc tacggggaca aaattttttg tcatttttga gcaacaacaa aaagattaga
     1561 agtaggaagt tttttggttt ttttactctt ggtgccaact aaaatttctc cactttttat
     1621 tatactaaaa cctgctcttt ttaccaattt ttttaattct ctctctgtaa aacaatgaaa
     1681 ataacaatca tcaactcctt cccatttcat taaaatatcc ttaaaatcta atttagattt
     1741 tcctaaaact tttgctttgg tttgttgaaa aattaaatct ctggtaaatt ttttctccca
     1801 taaattccaa actgtcaaaa tcaaaagtcc gtttttcttt aaaacccttt ttgcctctgc
     1861 taaaactctt aagcgaattt cttttgaagg aatatgatgc aacaatgcta tcgaataaac
     1921 cttatcaaaa tattcatcaa taaatggcaa gtttattgca tctcctactc gaaaatcaac
     1981 tccaggaaat ttatttttgg caatatcaat taactcttta gacacatcaa cccctatata
     2041 ttctatatct ttgccttcta acatttcata aaatctacca ttgccacaac caaggtctaa
     2101 aattctttct ttgcctttta cataatcatc aaacaaaaat ctcatttctt cccacgcctt
     2161 gccacgagcc ctcgcaaatt gcccgctcat tgtgttataa tcttgatgaa ccttttttaa
     2221 taaaaaattt gcatattcta tgtccataga tccaaatgaa attattatca aaaatttaat
     2281 caaaatcaat gctaaaaatc aatctgacct ggcacaaact aaaagggaaa tagctaaaca
     2341 ttataaagtc ccctgtcctt ctaatatcaa actattagaa gtttatcata atttgcttta
     2401 tactgacaaa atagaaaaaa atgagaactt ggaaaatctt ttaattaaaa gaaaaattcg
     2461 ttcgctatct ggagtagtgg tggtttcggt tttaaccaaa ccatatccct gtcccggaaa
     2521 atgcatttat tgtcctaaag aaaaaggaat tcctaaaagt tatctctcgg gtgaaccagc
     2581 cgtagaaaga gcaaaaaaat taaatttcga tccttattta caagtaaaaa aacgattgga
     2641 aatgctacaa aacgagggtc atccaactga taagattgaa ctgagaatta ttggaggaag
     2701 ttggagttgc tatccaaaag attataaaca atggtttatt actaaatgtt ttgaagcagc
     2761 aaacaaattt aataaaaatt ccaaattcca aattccaaat ttcaaacaag tttcaattgc
     2821 cgagccctgt aaatcaggac aagacgcgag aagagacgaa ttctctttga attccaaaca
     2881 aggggtgaaa agaggcgaag cctctttgaa ctccaagcaa ggggtgaaaa gagacgaagc
     2941 ctctttgaac tccaagcaag gcgcgaaaag agacgaagcc tctttaagct ccaatattca
     3001 aaaccttaaa caggagcaaa aacgcaatga aactgctaag catcgtattg ttggtatttc
     3061 cattgaaaca cggcctgatt taataaatga aaaagaaata aaagagttaa gaaaattagg
     3121 cgtaacccta gtggagattg gtgttcaaac catatttgat gatgttttga aaaaatgcca
     3181 acgtgatcat agtgtaaaag aggtcattga agcaactaaa cttttaaaag atgctggttt
     3241 taaggtgctt tatcaaatga tgccaaattt accaggatct acaatacaaa aagatcttgc
     3301 tacttttaaa gaattatttg aaaatcctca ttttaaacct gattggctga aaatttatcc
     3361 ttgcgttgtt tgcaaagaat caaaacttta tcaattatgg gaaaaaggta aatatcaatc
     3421 ttattctgat gaagagttaa ttaacctttt gataaaaatt aaagagattc ttccctattg
     3481 ggtaagggta gcaagattat ttcgcgatat tccttcttca aaaattcaaa gcggttcaaa
     3541 ggtttctaat ttaagagagg tagttttaaa taaattaaac caagatggca aaagttgtca
     3601 ctgcattaga tgccgcgaag taagagaaaa ttataaccca aaagaaaaaa tttatttaaa
     3661 aagagaggac tatgatgcct ctggaggcaa agaaatattt ttaagttttg aaaataaaaa
     3721 cagaacaaaa ttgtttgctt ttttaagatt acgtatcccc tcctttgttt tttcaagaga
     3781 aaaacacttt ttacctgttc ttcaagatgc agctattatt cgagaaattc acacctatgg
     3841 agcactaaca cctttcaata aaaaaacaaa cctttctcct cagcaccaag gaatgggaag
     3901 agctctaata aaagaagcgg aatacattgt aaaaaatgaa ttccgtcttc ctaaaattgc
     3961 tgtaatatct ggtgtgggtg tcagaaatta ctggcgaaaa ttgggctacc gtttaaataa
     4021 tacctatatg gtaaaaaatc aaatccttta aaaataacaa gattaaagct agctaagttt
     4081 gtcttttaac tcttttagga tttctgctgc aaggtctttt cctcccaatc caaaagccaa
     4141 tcctgttgct attgtaatca ttgcaatcat tccataaatc aaggccctca caatatctgg
     4201 tgtaattcct aattgtaaaa gaattgctaa aatagcaaaa acccaaattg cccatcgaat
     4261 gccagatccc aataatttag aataactgat ctttgctttt tcactggaag caacaacaat
     4321 tcgatatgaa aaatcagcca aaaaaactgc tactacaaaa atcaaagcag ccacaagaag
     4381 atttggaaga taagcaatca cctgtcctaa aaactcacta aattgaggaa gattaattac
     4441 ctcgcaagcc accattaaaa aaataatcac aaaacaccac tttacaattt ctccaaaaaa
     4501 ctttggcgca tctaaacgaa tctctgctct tgctaaagat tcttcccatc ccattctctt
     4561 taaaatttga ttaagcttaa atttatttaa aagagcagta actaattgcc ctattccacc
     4621 tgatactaac caaccaacta aaacaattaa tacagcaatt aaaaccctaa gcaagggccc
     4681 aaattgttca atgcttttcc aaaaaatatt aatatcagca ggcataaaat aattcattac
     4741 gaatttcaac taattacaaa tattcgtaat tagttttaat ccgtaatctg taatattttt
     4801 ttcgaccttt acctcgcaat agaaatttaa cttatctata caaagcaatt caagcaattg
     4861 ttgaaatttg ctaaaaatac cagttaaatt tcattacggg ttatttctct gcagcgaatc
     4921 tcgaatagaa aatcctgata tgaattttcg aaattctact gcaggacttg aattttgttt
     4981 tttaataata tattataaag gataacccaa aaatatacaa ggagttaata attctaagtt
     5041 ttaagataaa gaatcttgtt gtattgtcta gaattgaaag tcaaaattac caaagagaaa
     5101 cttaaatttt ttactgcact gatttcttaa ttttataact cttaaccata atattattgc
     5161 ggggtagaga agaagtatct cgtgggtccc ataagcccaa gaccctggtg caagtccagg
     5221 ccccgcgaaa gttttattaa ttttacctaa aattatggta aaataggcgg tgtagctcag
     5281 tggtagagca gtggcatcat aagccatgtg tcgtgggttc gagtccctcc accgctataa
     5341 ccttaggtag attcaatttt ttaa
//
LOCUS       DAOTQD010000033         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_13585_length_5099_cov_8.898493, whole genome shotgun sequence.
ACCESSION   DAOTQD010000033 DAOTQD010000000
VERSION     DAOTQD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13585_length_5099_cov_8.898493"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..2146)
                     /locus_tag="P5570_01970"
     CDS             complement(137..2146)
                     /locus_tag="P5570_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRR39501.1"
                     /translation="MQALNLFDLVEKTKVKKMIRLTDLKPINNLKLIFREIRDYFAGN
                     VTGITRDEKIAQNIMRLLFCKIYDEKNSDIVVEFSNRPNENYDIFYKRISNLFEKVKI
                     NYPGIFEQTEKIEIKPEELSFIVSKLEDYSIINADRDVVGDAFEELIGTSFRGGEGQF
                     FTPRNVVQMMIDVLSPQEGERVIDPACGSGGFLAYTLRHYISSNVKNFYLAGIDKDAF
                     LSKIAKIYLSLLGDDEFDIFCENSLEVPEKWSSDTKKKIELGTFDVVLTNPPFGAKIP
                     VIGKELLSQYQLGHKWENGNKWIQTNKLLDKQPPQVLFIERCLQLLSNGGRLGIVLPE
                     GIFGNPSDRYIWEYISSVASITSVVSLPQETFQPSTHTKTSVLFLKKTTERPKSIFMA
                     MANSVGHNKNGKPIYKIDKSDNKKVLDDDLPIIAKKFNSYKNNNKAGTDHLGFNISYD
                     ELNDHIFIPEYYNPEIIKEIETLKKSGKYLLLSIGNSIDKGVLQIRRGNEIGSQFYGT
                     GEVPFVRTSDIVNWEIKVDPVKAVSEEVYNQYKELQDIQENDILFVNDGTFLIGRTAM
                     VTKLDLKIIIQSHVRKIRVLDNEFIDPFYLFYLLNSKIVRKQIDSKTFVQATISTIGN
                     RLREVILPISKDQKEIKKIIKEIKDIIEKKADLREKIVKIVDNSI"
     gene            complement(2153..3160)
                     /locus_tag="P5570_01975"
     CDS             complement(2153..3160)
                     /locus_tag="P5570_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39502.1"
                     /translation="MATGRTISASFLTDLDHKVIELCDSISNTIYDSIPDGEAKKEFK
                     KEYGKISYTRDGGLGLGGGKIQRDAICTRGRQGKAPFSNRNLRWHPLIVAENKPDFAR
                     KIERIEIGGQDDGQILIFVIKDAEGKEFKFPSDKVHEMPERFVALPEHWYPHINILKH
                     WSDTLWTQNSCVIPALEACNWWDSVETYAVLGLALAVDLYRADFESLYDKIRQILAKQ
                     NIDKRVSLPTAIFPTKKEDIVKCPVCLLNISKDLEGFRTSERGKTWQPAWRSSKKEEG
                     DDSSIQIMHVNPLIESHIKHNASNVRYGHRWCNVSMTDHSLDETLDFMEFIVRAHKRC
                     K"
     gene            complement(3165..3371)
                     /locus_tag="P5570_01980"
     CDS             complement(3165..3371)
                     /locus_tag="P5570_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRR39503.1"
                     /translation="MTSGKVISENIKKLRAKLGLTQDDLAKKADIKYTTLMKVESGAV
                     NKPSVQTMAKIAKALGVSIEELIK"
     gene            3440..3757
                     /locus_tag="P5570_01985"
     CDS             3440..3757
                     /locus_tag="P5570_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39504.1"
                     /translation="MKSIKKKKSPLLAKIKFEPTFFQVLWRFAFSLPLQSEEGGRGVR
                     GEFRPPSPLMKSVRIFSNTHRQSENLVAGSPSARARQPILKEKFNFLSLFRRRRNARS
                     GLF"
     gene            3767..>5099
                     /locus_tag="P5570_01990"
     CDS             3767..>5099
                     /locus_tag="P5570_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRR39505.1"
                     /translation="MKYFIYCRKSTDSEERQILSIEAQLAELKEFAAKEKLEIVASLC
                     EAQTAKEPGRKIFGEMLDRICAGEASGILAWHPDRLARNSIDGGKIIYLLDTGKLLDL
                     KFPTFWFENTPQGKFMLSIAFGQSKYYVDNLSENIKRGHRQKLRKGIWPGFAPLGYLN
                     NHRTKEVDLDKDKAPFIKKAFELYATGDYTLKAIKEFLADNGITSYRNKLLSVSCVQR
                     MLKNHFYYGVFKFNGEVYQGIHEPIISKKLFDSVQQVMNNRGKKKRKRKHEFAFSGFM
                     HCGNCGCMITAEKQKGHNYYRCTKKKQKCDERYLREESLVEQMRNFIQKVSIPDDWAE
                     NMLAEIDKEKEQAKLESLSFVQNLHQKKAEVEQKIEKLLDLYIAGGALTLEEYQAKKQ
                     KLLNEKLDIEQKIRDFEQSGNNWLEPMKKMIFEAKQAKILLSQGDNQQILTF"
BASE COUNT         1565 a          999 c          848 g         1687 t
ORIGIN      
        1 aattcccccc gaactcccct tccgccctcc tcgctctgaa gcggaagcga aaaggacgat
       61 ttcaagtttt tcgcgggcgt agccccgcaa ggaaaatcat aactgatttt cctctttggc
      121 ggcaaattct cttttatcaa atactattat ctacaatctt aactattttt tctcttaaat
      181 cagctttttt ttcgataata tccttaattt ctttaatgat tttttttatt tctttctgat
      241 ctttacttat tggcaatata acctctctca atctgttacc aatagttgaa attgttgctt
      301 gtacaaatgt ttttgaatct atttgttttc taacaatctt actattcaaa agataaaaca
      361 agtaaaatgg atcgataaac tcattatcta atacccgaat ttttctcaca tgactttgaa
      421 taattatttt taaatctaat ttagttacca ttgccgtgcg tccaattaaa aatgtaccat
      481 catttacaaa taaaatatca ttttcttgaa tatcttgaag ttctttatat tggttataga
      541 cttcctctga aacagccttt actggatcaa ctttaatttc ccagttgaca atatcgctag
      601 tccttacaaa tggcacttct cctgtgccat aaaattgaga tccgatttca tttcctcttc
      661 tgatttgtaa aaccccttta tctattgaat tgccaataga taataataaa tattttccgc
      721 tttttttaag cgtttctatc tctttaataa tttcaggatt gtaatattcg ggaataaaaa
      781 tatgatcatt taattcgtca tatgaaatat tgaatcccaa atgatcagtg ccagctttat
      841 tattgttttt atatgaatta aactttttgg caattatagg aagatcgtcg tctaatactt
      901 ttttattatc agatttatct attttataaa tcggtttccc gtttttatta tggccaacgc
      961 tatttgccat agccataaaa attgattttg gtctttcagt tgtctttttt aggaataaaa
     1021 cacttgtttt tgtatgagtg ctcggttgaa aagtttcctg aggtaaactt acaacactag
     1081 taattgacgc aacgcttgaa atatactccc aaatatacct atcagatgga ttgccaaaga
     1141 ttccttcggg caaaacaata ccgagtctcc caccattact aagcaactgt aagcatctct
     1201 caataaataa gacttgaggg ggttgtttat caagtaattt atttgtttgt atccatttat
     1261 taccgttttc ccatttatga ccgagttgat attgacttag taattccttt ccaataactg
     1321 gtatttttgc gccgaaaggc ggatttgtga gcactacgtc aaaagtgcca agttcaatct
     1381 tttttttagt atcactgctc catttttcag gcacttctaa actattttca caaaagatat
     1441 cgaattcatc atcgccaagt aacgagagat aaatttttgc gatttttgat aaaaacgcat
     1501 ccttatcaat accggcgaga taaaaattct ttacattact tgaaatataa tgtcttaggg
     1561 tataagcaag aaagccacca ctgccacaag ccggatcgat aacccgctct ccttcttggg
     1621 gcgacaaaac atcaatcatc atttgcacaa catttcgcgg tgtaaaaaat tgcccttcgc
     1681 ctcctctaaa agaagttcct atcaattctt caaaggcatc cccaacaaca tctctatctg
     1741 cgtttataat tgaatagtct tctaattttg agactataaa agaaagttcc tctggcttta
     1801 tttctatttt ttcagtttgt tcaaaaattc ctgggtaatt tattttaact ttttcaaata
     1861 aattgcttat tcgtttgtaa aaaatatcat aattttcatt aggacgattg gaaaattcta
     1921 ctactatatc actgtttttt tcgtcataaa tcttacagaa aagaagtcgc attatatttt
     1981 gcgcaatttt ttcatccctg gtaatgcccg taacattacc agcaaaataa tctcggattt
     2041 cacgaaatat taacttgaga ttatttatcg gctttaagtc agttaacctt atcatttttt
     2101 taacttttgt tttttcaacc aaatcaaata aatttagtgc ttgcataaga aattatttac
     2161 accttttatg tgcgcgtaca ataaattcca tgaaatccaa agtttcatct aacgagtggt
     2221 cggtcataga aacattacac catctatgcc catatctaac attgcttgca ttatgtttaa
     2281 tatgactctc aataagggga tttacatgca ttatttgaat acttgaatca tcgccctctt
     2341 cttttttaga agaacgccat gctggctgcc atgttttacc tctttcagat gttctaaatc
     2401 cctctaagtc ttttgagatg tttagaagac aaacagggca cttaacaata tcttctttct
     2461 ttgttgggaa aatagctgtt ggtaatgaaa ctcttttatc aatattttgt tttgctagta
     2521 tctgcctaat tttgtcataa agtgactcaa aatcagctct gtacaaatca acagctaaag
     2581 caagacccaa aactgcataa gtctcaacag agtcccacca gttacacgct tcaagggcag
     2641 ggattacaca agagttttgt gtccatagtg tatcggacca atgttttaaa atattaatat
     2701 gcgggtacca atgttctgga agcgcaacaa atctttccgg catttcatgc accttgtctg
     2761 aaggaaattt aaactcttta ccttctgcgt ccttaataac aaaaatcaaa atctgcccat
     2821 catcttgacc gccaatttct attctttcta tttttcttgc aaaatcaggt ttattttcgg
     2881 cgacaattaa aggatgccac cttaaatttc tattcgaaaa aggagctttg ccctgccgac
     2941 ctcttgtgca tatggcgtct cgttgtattt tcccaccgcc aagtcctaac ccaccatctc
     3001 tggtataact gatttttccg tattcttttt tgaattcctt tttagcttcc ccatcaggta
     3061 tagagtcata aattgtattt gatattgaat cgcacaattc aataacttta tgatctaaat
     3121 ccgtaagaaa gcttgcactt attgttcttc ctgtagccat atttttattt tattaactcc
     3181 tcaatgctca caccaagcgc tttggcaatt tttgccatag tttgaacgct tggcttatta
     3241 accgctccgc tttcgacctt cataagcgtt gtgtatttaa tatccgcttt cttggctaaa
     3301 tcatcttgcg ttaagccaag ttttgctctt aattttttta tgttttcgct tataactttt
     3361 ccacttgtca tactaataaa gttttggtag tataatagta aatgataatt ttacatctat
     3421 tattatatta ccaaaaaata tgaaatcaat caagaaaaag aaaagcccgc ttctggccaa
     3481 aataaagttc gagccgacat tttttcaggt tctttggcgg tttgcctttt cgcttccgct
     3541 tcagagcgag gagggcggaa ggggagttcg gggggaattc cgcccgccga gcccgttaat
     3601 gaaatcggtt cggatttttt caaatacaca ccgccaatca gaaaatcttg ttgctggctc
     3661 gccctctgcg agggctcgcc agccgatttt gaaggagaaa tttaattttt tgtctttgtt
     3721 ccgccgaagg cggaacgcca gaagcgggct tttctaaact aaaactatga aatactttat
     3781 ctattgcaga aaatcaactg actcggaaga aaggcaaatt ttgtctattg aagctcagct
     3841 tgctgagcta aaagaatttg ccgccaaaga aaaacttgaa atcgtcgcct cgctttgcga
     3901 ggcgcaaacc gccaaagaac ctggccgaaa aattttcgga gaaatgttag accggatttg
     3961 cgctggcgaa gccagcggaa ttttggcttg gcatccggac aggttagcaa gaaattctat
     4021 tgatggcgga aaaattattt atctgctgga cacaggaaaa ttgcttgatt tgaaattccc
     4081 gactttctgg tttgaaaata ctccgcaggg aaaatttatg ctttctattg cttttggaca
     4141 aagcaaatac tatgtggaca atttaagcga gaatatcaaa agaggccacc gccaaaaact
     4201 gcgaaaagga atttggcccg gcttcgcgcc actcggctat ttaaacaacc atcggacaaa
     4261 agaggttgat ttggacaaag acaaagcgcc gtttatcaaa aaagcgtttg agctttacgc
     4321 taccggcgac tataccctaa aagccataaa ggagtttttg gctgataatg gaataacaag
     4381 ctaccgcaac aaactgcttt ctgtttcctg tgtccagcga atgctcaaaa atcatttcta
     4441 ttatggagtg tttaagttta acggagaagt ttatcaagga attcatgagc caattatttc
     4501 caagaaactt tttgattctg tccagcaagt aatgaataac cgaggcaaga aaaaaagaaa
     4561 acgaaaacac gaatttgcct tttccggctt tatgcattgc ggaaactgcg gctgtatgat
     4621 aaccgctgaa aaacaaaaag gccataacta ttaccgttgc accaagaaaa agcaaaagtg
     4681 cgatgaaagg tatttgagag aagagagtct tgttgaacag atgagaaatt ttattcaaaa
     4741 agtttctatt cctgacgact gggcggaaaa tatgcttgcc gagatagaca aggaaaaaga
     4801 gcaagccaag ttagagagtt tgtcttttgt tcaaaatctc caccaaaaaa aagcggaagt
     4861 tgagcagaaa attgaaaaat tgttagacct ctatattgcc ggtggagctt tgactttgga
     4921 agaataccaa gctaagaaac aaaaactgct caacgagaaa ctggacattg agcagaaaat
     4981 cagagatttt gaacaatcgg gaaataattg gctcgaaccg atgaagaaaa tgatttttga
     5041 ggcgaaacaa gccaaaattc tattgtcgca aggcgacaat cagcaaatcc taacatttc
//
LOCUS       DAOTQD010000034         4511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_15718_length_4511_cov_7.868268, whole genome shotgun sequence.
ACCESSION   DAOTQD010000034 DAOTQD010000000
VERSION     DAOTQD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15718_length_4511_cov_7.868268"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..630
                     /gene="tsaE"
                     /locus_tag="P5570_01995"
     CDS             166..630
                     /gene="tsaE"
                     /locus_tag="P5570_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRR39506.1"
                     /translation="MLEFITNSPKKTENLGKNFAEKIIRRHLKLNSQKAIIISLEGDL
                     GGGKTTFTKGFARGLGIKDNIKSPTFIIFRKFQIPHFKQKPGPKTFYHFDCYRIEDPK
                     EILDLEFKKIIENPQNIVIFEWGDKIKKILPDDCFKIKFEFLDKNKRKITIS"
     gene            897..1826
                     /locus_tag="P5570_02000"
     CDS             897..1826
                     /locus_tag="P5570_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRR39507.1"
                     /translation="MGGLLIIDSNALLHRSFHALPPLTNSHGEPTGAVYGFLLTFLKA
                     INDLNPDFIVATFDTPSPTFRHKKFKAYKAQRPKTPDDLIQQIAKTKEILTAFSVPIF
                     EKEGFEADDLIATIVKKTQQEQIDQKLKVYILTGDFDTLQLVNDQVKVYTLGRGVRDT
                     IIYDKEKIKERFGLEPRQMVDFKALAGDPSDNIPGADGIGQKTAIGLLKKYDNIENLY
                     QKIEENRADDLKPRIKNILLKNKEQVFFSQVLAKTRDDAPIDFDLKKCEFGNFDKEKA
                     EEILKKFEFYSLLKRIPEIKKDLSLPKKEGRLF"
     gene            1844..3172
                     /locus_tag="P5570_02005"
     CDS             1844..3172
                     /locus_tag="P5570_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39508.1"
                     /translation="MRILIAYYSRTGNTEKVAQALKKEFEHRHQYVDVEKIEPQEEYT
                     FWGWWRLKIAKRGCKIKEPKIKDVSKYDVVLIGSPNWTGLSLPVTQYLKKIRGLKNKY
                     VAVFSTSFLPPKIGWYMLSGYFFDYTTNAIIKEKRARLIAWISLSSKFKKWNIESNYG
                     QECLNNFCDTIESPTPPLKKFLLDEEEEEEIRSVMMIFLKGLFLFLIFQIITCLFGWE
                     IFTLSQFLIIFAGGLIISFLLLWTLFNRRWLFLGKYVTCVGLITLWGIAMFFLQPANY
                     LPLLGCILVTVAMSFFKDYRVVGITGIAAVIFYLFFYLSFPALGEVAVFNMDLVFLFL
                     VVVINLFISQNLQNDFVNLAEAQEEIETTNEVLEIRVRARTRALRTLAESLDVEVKKR
                     TKELQERIDELEKFHKLTVGRELKMIELKKSLRQAQEEIKKLKKEIEKKS"
     gene            3235..>4511
                     /locus_tag="P5570_02010"
     CDS             3235..>4511
                     /locus_tag="P5570_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR39509.1"
                     /translation="MEKNIEKTKKENVEELTALLTQRERELIKLTKALEQAKERELIL
                     KIRERAKSEDAERRLKELEDTRKALLNILEDVEEAQRRAEEEKEKTEAVITNLADGLF
                     VFDVEGRVSMINPQAEIFFGIKRQDIEGMTVSDLMNIPVLKSFAELIGNEIRKTFREE
                     IKIKDRTLEVTTVPVMHKGDKLGVLAIVHDVSREKLIEQMKTEFVSIAAHQLRTPLSA
                     IKWSISLLKEKIKGDKDAEDLLEKLNQSNERMIRLIDDLLNVTRIEEGRYIYKPVPRD
                     IVQIIESVIVLRVEAAKRKGIDFSFNKPKEKIPEVKVDEEKISIVIQNLVENAINYTP
                     SGGKILVSIIYQKETQEILVEVKDTGIGIPKSEQQRVFSKFFRGTNAIKNETEGTGLG
                     LFIAKNIIEAHGGKIWFKSEKDKGTTFYFTLPI"
BASE COUNT         1657 a          611 c          855 g         1388 t
ORIGIN      
        1 catcgggatt gccacgtcgt ccactcacct tttactaagg tgagggactc ctcgcaatga
       61 cagagaggcg tgccacggga ttgccacccc ctcacttttg tgaaggtgag ggatcgcaat
      121 gacaattatt gaaaattagt aaattggtaa attcattaga aatttatgct tgaatttatt
      181 actaattctc caaaaaaaac tgaaaattta ggaaaaaatt ttgccgaaaa aattattcgc
      241 cggcatttaa aattaaattc gcaaaaagca attattatct cattagaagg tgatcttggc
      301 ggagggaaaa caacatttac caaaggattt gccagaggac ttggaataaa agataatatt
      361 aaaagcccta cctttattat ttttagaaaa tttcaaattc cgcatttcaa acaaaagcca
      421 gggcctaaaa ctttctatca ctttgattgc tatcgaatag aagatccaaa agagatttta
      481 gatttggagt ttaaaaaaat tattgaaaat cctcaaaaca ttgttatttt tgaatgggga
      541 gataaaatca aaaaaattct gcccgatgac tgtttcaaaa ttaaatttga atttttagat
      601 aaaaataaga gaaaaataac aatatcataa aatatgatgg gattgaaatt ccattatatt
      661 ctctatcgtt tttattttat ggtataatta aaaataccat ccaatgatta cgattgcaaa
      721 ttttaaatgt cgagatttat ccatcgagga gaaaatattt ttaaataaga ttttgttttt
      781 ttattctttt ttattctttt ctttacaaac taaataaaaa ttcagtaata atggaaatgc
      841 gcgctaaata atgtagagag aagagaaaaa acaagatcaa atcatcttaa aaaactatgg
      901 gaggattgct tatcattgat tctaatgctc ttttgcaccg ttcattccat gccctgccgc
      961 ctttaaccaa cagtcatggt gaacctacgg gcgctgttta tggtttttta ttaacatttt
     1021 taaaggcaat taatgatttg aatcctgatt ttatagtagc tacttttgac acgccctctc
     1081 ccaccttccg gcacaaaaaa tttaaagcat ataaagctca aagaccaaaa acgcccgatg
     1141 atttgataca gcagattgct aaaacaaagg aaatattaac agctttttct gttcctattt
     1201 ttgaaaaaga aggatttgaa gctgatgatt taattgcaac aattgttaaa aaaacccagc
     1261 aagagcagat tgatcaaaaa ttaaaagttt atattttaac tggtgatttt gatacccttc
     1321 agcttgtgaa tgatcaagtg aaggtttata ctttgggcag gggagtaagg gatactatta
     1381 tttacgataa agagaaaatt aaagaaagat ttggccttga gccacgacaa atggttgatt
     1441 ttaaagcctt ggccggagac ccttcagata atattcccgg ggctgatggt attggacaaa
     1501 aaacagctat tgggttgctt aaaaaatacg ataacataga aaatctttat caaaaaatag
     1561 aagaaaacag agccgatgat ttgaaaccgc ggataaaaaa tattttacta aaaaacaaag
     1621 agcaggtttt tttctctcaa gtgctagcaa aaacccggga tgacgcacct attgattttg
     1681 atttaaaaaa atgtgaattt ggcaattttg acaaagagaa agcagaagaa attttaaaaa
     1741 aatttgaatt ttatagtttg ctaaaaagga ttcctgagat aaaaaaggat ttatcattgc
     1801 ccaaaaaaga aggaagatta ttttaatttt tagattttat tatatgcgta ttcttattgc
     1861 ttattattca agaacaggaa atactgaaaa agtagctcag gcgttaaaaa aagaatttga
     1921 gcatcgtcat caatatgtag atgtagaaaa aatagaacct caagaagaat atactttttg
     1981 gggctggtgg cgtttaaaga ttgccaaaag agggtgtaaa ataaaagaac caaaaattaa
     2041 agatgtttca aaatatgatg ttgttttgat tggatctccc aactggaccg ggctttcttt
     2101 gccagtgaca cagtatttaa aaaaaataag aggattaaaa aataaatatg tagctgtttt
     2161 ttctacatca ttcttgccac caaagattgg ttggtatatg ctttctggct atttttttga
     2221 ttatacaact aatgcgatta taaaagaaaa gagagccaga ttaatagctt ggataagctt
     2281 atcatctaaa tttaaaaaat ggaacattga atcaaattat ggtcaggagt gtttaaataa
     2341 tttttgcgat acaattgaat cgccaacgcc acctttgaaa aaatttttat tggacgaaga
     2401 agaggaagag gaaattcgat ctgtgatgat gatattttta aagggtcttt ttcttttttt
     2461 aatttttcaa attattactt gtctttttgg atgggagata tttaccctat ctcaattttt
     2521 aataattttt gctggcggtc ttattatctc ttttctttta ttatggactc tttttaacag
     2581 gcgatggtta tttttgggaa agtatgtgac gtgtgttgga ttgatcactc tttggggtat
     2641 agcaatgttt ttccttcagc ccgctaatta tttaccactg cttggatgta ttttagtgac
     2701 agtagcaatg agttttttta aggattatcg agtggtcggt ataacaggca ttgccgctgt
     2761 cattttttat ctattttttt atctttcgtt tccggccctg ggagaggttg ctgtttttaa
     2821 tatggacctg gtgtttttat ttttggttgt tgtaattaat ttatttatta gtcagaatct
     2881 tcaaaatgat tttgttaatc ttgctgaagc ccaggaagag attgagacaa caaatgaggt
     2941 gcttgaaatc agggtaagag cgcggacgag ggctttaaga accttagctg aaagtttgga
     3001 cgtagaggta aaaaagcgaa ctaaagagct tcaggaaaga attgatgaat tagaaaaatt
     3061 tcataaatta actgtgggca gggaattaaa aatgatagaa ttgaaaaaat cattacgcca
     3121 ggcgcaagag gagataaaaa aactaaaaaa ggaaatagag aaaaaatctt aaacaaattt
     3181 tatgttgact cttgaataca atcgagaagt gtctgatcaa tttaatattt aataatggaa
     3241 aaaaatattg aaaaaacaaa aaaagaaaat gtagaagaac ttactgctct tttgactcaa
     3301 cgagaaaggg aattgataaa actaacaaaa gccctggagc aagcaaaaga aagagaatta
     3361 attttaaaaa tcagggagcg ggcaaaatct gaggatgctg aaagaagatt aaaagaatta
     3421 gaagatacta gaaaagcctt gttaaatatt ttggaagatg tggaagaagc ccaaagaaga
     3481 gcagaggaag agaaagaaaa aactgaagca gtgattacta atcttgctga tgggcttttt
     3541 gtatttgatg ttgaaggcag ggtttctatg ataaaccctc aagcagaaat tttttttggc
     3601 attaaacggc aagacattga gggtatgaca gtttcagacc taatgaatat tcctgttttg
     3661 aaatcttttg cagaattaat tggtaatgag attagaaaaa cttttcgaga ggaaattaaa
     3721 ataaaggacc gaacattaga agtaaccact gttcctgtta tgcataaagg ggacaaacta
     3781 ggagttttgg caatagtgca tgatgttagt cgggaaaaat taatagagca aatgaagacc
     3841 gaatttgtat ctattgctgc tcatcaattg cgtactccgc tttcagcaat aaaatggagc
     3901 atttctttac taaaagaaaa aataaaaggt gataaagacg ccgaggactt attagaaaaa
     3961 ttaaaccaat ccaatgaacg aatgattcgg ttgattgatg atcttttaaa tgttactagg
     4021 atcgaagaag gacgatatat ttacaaaccc gtccccaggg acattgttca aattattgaa
     4081 agtgttatag tattgagagt tgaagcagct aaaagaaaag gcattgattt ttcttttaat
     4141 aaaccaaaag aaaaaattcc agaggtaaaa gtagacgagg aaaaaatttc tattgtgatc
     4201 caaaacttgg tagaaaacgc catcaactac actccaagcg gtggcaagat tttagtctca
     4261 ataatatatc aaaaagaaac gcaagaaata ttagttgaag taaaagatac aggcattggt
     4321 attccaaaat cagaacagca gcgggtgttt agtaaatttt ttaggggcac caatgctata
     4381 aaaaacgaaa cagaagggac aggattagga ctttttattg ctaaaaatat tatcgaggcg
     4441 catggtggga aaatttggtt taaatcagaa aaagacaaag gtacaacttt ttattttact
     4501 ctgccaatca g
//
LOCUS       DAOTQD010000035         4183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_17202_length_4183_cov_8.086483, whole genome shotgun sequence.
ACCESSION   DAOTQD010000035 DAOTQD010000000
VERSION     DAOTQD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17202_length_4183_cov_8.086483"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="P5570_02015"
     CDS             complement(<1..398)
                     /locus_tag="P5570_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRR39510.1"
                     /translation="MSVETVFIMILIGIFTGVVTGLTGASGVMVVVPLVNIFLSFSIH
                     EAIGTSLIVDVLSPLAISYIYYKHGNLDIKSGIWIAVGSILGAQLGAGFAAGIPEMGL
                     SGAFGIFMIIMGVIIWQKGLSHQSLTKIIN"
     gene            complement(395..1399)
                     /locus_tag="P5570_02020"
     CDS             complement(395..1399)
                     /locus_tag="P5570_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRR39511.1"
                     /translation="MSSTVKTTLKVVGVLVAIAVLYFIRDVLGYLLLSFVFASALKPG
                     VNFLEKKNIPRTFSAILIFLLFLIFLGALFYLVLPPLINEVQNFISTFPQYWENFLSW
                     LPQFEDWLEKMPFGQNIANTINQSIEGLSKAVGSVIGVIYGFFGQVFDFFFILIVAFY
                     LLIEKDVGGRLSQLFFSKNDQTKTKISKYCNLAEKIAGRWLQGYIFLGIIVGILVYIG
                     LLILGAKYALILAVLAGALEIIPFFGPVFAGVAGFILCFFQGGWTMALWAAFIFFIVQ
                     QLENYLIVPMIMKNRVDLNPLLTIIVLFIGGRLGGFLGMILAVPITAILLAFLKERKV
                     "
     gene            complement(1372..2634)
                     /locus_tag="P5570_02025"
     CDS             complement(1372..2634)
                     /locus_tag="P5570_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRR39512.1"
                     /translation="MQKTIFKNGLRVITVPMKGTKTVTVLVGVGAGSRYETKKINGIS
                     HFLEHMLFKGTKKRPNSQAISEELDAVGGEFNAATGAEQTYFYAKVGSRYQNLALDVI
                     SDIILNSILKEEEINREKGVIIEEGNMIKDTPMQYVGELFEKLLYGKETVGWNILGEK
                     ETILKMERKDFLSYINDYYVAQNIVVCVAGNINQKNTLNEVKKYFTNIKEKKPKKRKK
                     IKDFQTKPESLVQFKKTDQAHFCLGVRAYNIFHPDKYALSLLSVILGGNMSSRLFVKV
                     REKEGLAYYIRTNVETYTDTGYLVTQSGVDNQKIEKAIKIILNEYKDIREKGVNEKEL
                     KRAKEYIKGKMLLKLELSDEMAAFFANQEILTNKILTVEEKFAKIDKVTVKDIQRVAK
                     DIFVPQKLNLALIGPFKKNLCHQLLKQL"
     gene            complement(2756..4168)
                     /locus_tag="P5570_02030"
     CDS             complement(2756..4168)
                     /locus_tag="P5570_02030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRR39513.1"
                     /translation="MDQIEKIVSLCKRRGFIFPSSEIYGGFGSCYDFGPLGVELKNNI
                     KKLWWDEMTKMHNNIVGLDASILMSPKVWQASGHTKNFTDPLVECKSCHKRFRADHLI
                     EKFKNSQKIDEHSKFTEEELKDITKMVSEIKCPECKGELTPPRQFNLMFKTFIGPVED
                     EASVAYLRPETAQGIFVNYKNILDSQRVKTPFGIAQIGKAFRNEITPGNFIFRLREFE
                     QMELEYFVKPGEDEKIFDYWVEERVKWYVEILGIKQENLRIRPHEKEELAHYAKSCVD
                     IEYNFPFGWSEIEGIANRTDFDLIQHQKSSGVDLRYFDEKEKKYYIPYVIEPSSGVER
                     ILFAVLCDSFEEISGGRTRTTKSTKEIETVLRFNKKTAPVKIAILPLVKNKPEIVEKA
                     KEIYHLLKPYFVCQYDEKGSIGRRYRRQDEIGTLYCLTIDFESLKNNDVTIRNRDTMQ
                     QERVKTKDLKQKLTQLLNTY"
BASE COUNT         1429 a          838 c          513 g         1403 t
ORIGIN      
        1 ttgtttatta tctttgttaa agattggtga cttagtccct tctgccatat tattacaccc
       61 attattatca taaaaattcc gaaagcacca cttaaaccca tctcaggaat tccagcagca
      121 aaacctgctc ccaactgagc gcctaaaatt gaaccaacgg ctatccatat accagatttt
      181 atatcaagat ttccgtgttt gtaatagata taagagattg ctaaaggaga tagaacatca
      241 acgattaaac ttgtacctat tgcttcgtgg attgaaaaac ttaaaaaaat atttaccagg
      301 ggcaccacta ccattactcc cgaagcacca gttaaacccg tcaccacacc agtaaatatt
      361 ccaattaaaa tcattataaa aacagtttcc acgctcatac ttttctttct ttcaaaaagg
      421 caagcaaaat agcggttatt ggcactgcta aaatcattcc caaaaatcct cccagtctac
      481 caccaataaa taaaacaata atagtcaaaa gcggatttaa atcaaccctg tttttcatta
      541 tcataggaac aattaaataa ttttctaatt gctgaacaat aaaaaatata aaagcggccc
      601 atagagccat tgtccatccc ccttggaaga aacacaaaat gaagcccgct acaccagcaa
      661 atactggtcc aaaaaaagga ataatttcta aagctcctgc taataccgct aaaattaaag
      721 cgtattttgc tcccaaaatc aaaagaccaa tataaactaa tatgccaaca ataattccaa
      781 gaaaaatata accctgcaac cagcgaccag caattttctc tgctagatta caatactttg
      841 aaatctttgt ttttgtttga tcatttttag aaaagaataa ttgggaaaga cgccctccta
      901 cgtccttttc tattaataaa taaaacgcaa caattagaat aaaaaagaaa tcaaatacct
      961 ggccaaaaaa accataaata acgccaatca cagaaccaac cgcctttgac agtccctcta
     1021 tagattgatt aatagtatta gcaatatttt gcccaaaggg cattttttct aaccaatctt
     1081 cgaattgagg taaccaacta agaaaatttt cccaatactg ggggaaggta gagataaaat
     1141 tttgaacctc atttattaac ggaggtaaaa caaggtaaaa caaagcgcct aaaaatatca
     1201 aaaataaaag aaaaatcaat attgcagaaa acgttcttgg aatattcttt ttttccaaaa
     1261 aatttacacc cggctttaaa gccgaagcaa aaacaaaaga caaaagtaaa tatcccaaca
     1321 catcgcgaat aaaatataaa accgctatcg ctaccaatac accaactacc tttaaagttg
     1381 ttttaacagt tgatgacata gatttttctt aaaaggcccg attaaagcca aatttaattt
     1441 ttgcgggaca aaaatatctt ttgccacacg ttgaatatct ttaactgtta ccttatcaat
     1501 tttagcgaat ttttcttcca cggttaatat tttattggtt aaaatttctt gatttgcaaa
     1561 aaaagcagcc atttcatcag acaactccaa tttcaacaac atttttcctt ttatatattc
     1621 ttttgctctc tttaattctt tttcgtttac tcctttttct cgaatatcct tatattcgtt
     1681 taaaataatt ttaattgctt tttcaatctt ttgattatcc acaccagatt gagttaccaa
     1741 atagccagta tcagtatagg tttcaacatt tgttctgata taataagcaa gaccttcttt
     1801 ttctcttact tttacaaaaa gccgtgatga catattgcca cccaaaatta cagaaagcaa
     1861 actcaaggca tatttatcag gatggaaaat attataagcc ctcacaccta aacaaaaatg
     1921 ggcttgatca gttttcttaa actgaaccaa actttctggc tttgtttgaa aatcttttat
     1981 tttttttcgt tttttcggtt ttttttcttt tatatttgta aaatattttt taacttcatt
     2041 caaagtattt ttctgattga tattaccagc cacacataca acaatatttt gagcaacata
     2101 ataatcatta atataactta agaagtcctt tctttccatc tttaaaatgg tttctttttc
     2161 acccaaaata ttccatccta cagtttcttt gccgtaaagt aatttttcaa aaagttctcc
     2221 cacgtattgc atcggtgtat cctttatcat atttccttct tcaattatca cacccttttc
     2281 ccgatttatt tcctcttctt ttaatattga atttaaaata atatctgaaa taacgtccaa
     2341 ggcaagattt tgatatcttg atcctacttt tgcataaaaa taagtttgct cagcgccagt
     2401 agcagcatta aattctcctc ctactgcatc aagttcttca gaaatggctt gagaattagg
     2461 tcgttttttg gtgcccttaa acaacatatg ttccaaaaaa tgagaaatac cgttaatttt
     2521 ttttgtttca taacgcgagc cagcaccgac ccctactaaa accgttactg tcttagttcc
     2581 cttcattgga acagtaatca cccgaagtcc gtttttaaaa atagtttttt gcatagataa
     2641 aataaatcaa aaatcaaaaa tcaaaaatca aaatgataaa tcaaaattca aaaaaagatt
     2701 aaaagataaa aaataaaaag aaataattct ttttgatttt tgcattggtg tttaattaat
     2761 aagtgtttaa tagttgtgtt agcttttgtt ttaaatcttt tgtttttact ctttcttgct
     2821 gcattgtatc gcggttacga atagttacat cattgttttt caagctttca aaatcaatag
     2881 ttaaacaata taaagtacca atttcatctt gtcttcgata acgccgacca atagatcctt
     2941 tttcatcata ctggcataca aaatatggtt ttaataaatg gtaaatttct tttgcttttt
     3001 caacaatttc tggtttattt ttaactaaag gtaaaattgc gatttttact ggtgcagttt
     3061 ttttgttaaa acgaagcacg gtttcaatct cttttgtcga tttagtagtc cttgtcctgc
     3121 cacctgaaat ctcctcaaaa gaatcacaca aaacagcaaa taatattctt tcaacgccag
     3181 aagatggttc aatcacataa ggaatataat attttttttc tttttcgtca aaatatctta
     3241 aatctacgcc tgaagatttt tggtgctgta tcaaatcaaa gtcagtccta ttagctatgc
     3301 cctcaatctc tgaccaccca aaaggaaaat tatattcaat atcaacacaa ctctttgcat
     3361 aatgagccaa ctcttctttt tcgtgaggtc taattcttaa attttcctgc tttattccta
     3421 aaatttcaac ataccatttc actctttctt ctacccaata atcaaagatc ttttcatcct
     3481 ctcctggttt tacaaaatat tctaactcca tctgttcaaa ctctcttaac cggaaaataa
     3541 aatttcctgg tgtaatctca ttacgaaatg ctttaccaat ttgagcaatt ccaaaaggcg
     3601 tttttaccct ttgactgtca agaatgtttt tataattaac aaaaattccc tgagctgttt
     3661 ctggacgtaa ataagcaaca gatgcttcgt cttcaaccgg tccaataaag gttttaaaca
     3721 ttaaattgaa ctggcggggc ggggttaact cgcccttgca ttcaggacac tttatttctg
     3781 aaaccatctt tgtaatatct ttcaactctt cttcagtaaa cttactatgc tcatctattt
     3841 tttgagaatt tttaaacttt tcaattaaat ggtcagctcg aaaacgcttg tgacaggatt
     3901 tgcactcgac caagggatca gtaaaatttt ttgtatgacc agaggcctgc cacacctttg
     3961 gcgacattaa aatcgaagca tcaagcccca caatattatt atgcattttt gtcatctcat
     4021 cccaccacaa ttttttaata ttatttttca attctacccc taaaggccca aaatcataac
     4081 aggaaccaaa accaccataa atttctgatg aaggaaaaat aaatccccgg cgcttacaaa
     4141 gagaaacaat tttttctatt tgatccataa atttaacacc tcc
//
LOCUS       DAOTQD010000036         4016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_18035_length_4016_cov_8.721030, whole genome shotgun sequence.
ACCESSION   DAOTQD010000036 DAOTQD010000000
VERSION     DAOTQD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18035_length_4016_cov_8.721030"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /gene="smpB"
                     /locus_tag="P5570_02035"
     CDS             complement(<1..443)
                     /gene="smpB"
                     /locus_tag="P5570_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRR39514.1"
                     /translation="MSKIFAKNKRAYFDYEIIEKYEAGIELYGFEVKSIKTGRLSLKG
                     SFAIIKNMEAFLINAYIPPYQPNNTPKDYDPLRTRKLLLKKSEIKSLIGKQKQRGLTL
                     VPLRAYNRNGKIKIEIGLAKSKKKIDKREKIKKREFEREKERAMK"
     gene            619..987
                     /locus_tag="P5570_02040"
     CDS             619..987
                     /locus_tag="P5570_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRR39515.1"
                     /translation="MSKSLGQIGENIAAQYLQKKGYIILEQNYKKPWGEIDIIVKKGK
                     DIIFVEVKTVSNNNFLPEEEITPRKKKILIRTAKLYLAEKRYPADQDWQIDVIGIEMK
                     DNNKCLLRHTENAIGENSKL"
     gene            complement(1156..3714)
                     /locus_tag="P5570_02045"
     CDS             complement(1156..3714)
                     /locus_tag="P5570_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRR39516.1"
                     /translation="MPIRKYYRTTIEDKHKRKKDKKHRSWPKIVGGVFIFLLLVGIGV
                     FAYFASKTPNIEDLDERIIGESTKIYDRTGSYLLYEAGEKKKTSVSLDQISPLLQKAT
                     LAAEDDDFYSHPGIDFKAIVRAIIYDVLHLGKSTQGGSTITQQLIKNVVFLETETNEE
                     GKTEIVGPAPKTITRKIQEAIMALETERKYSKDQILEAYLNQISYGSVFYGAEAASQG
                     YFQKSASELTLAEAATLAAMTQSPTYYLKNPEDREERKNWVLDRMVELKFTTKEEAEE
                     AKKQKITLSPMSQPRKAPYFTSEVEKQLEEMYGEYDQMGLRVFTTIDLDLQSLAENTV
                     SDWAPTVENRYGAGNVALVSINPNNGEILAMVGGKNFEESQVNIWEYGFQSPGSAFKP
                     IVYATAFKKGYTPDTILWDVKTVFDEKKPDWPDNYDLKQRGPIKMKEALAQSLNIPAV
                     KTLYLAGINETINTAKSMGMIDTFDSELDKNSPNLSMAIGGKDVMPLELVSAYGVFAT
                     EGIRYLPNYILKIENARGEIIYEPESHPIQALDSEVCRQINSILSDDSLRAPMFGSRS
                     KLYLNSWTAVKTGTASTEKGKITDAWTIGYTKDLVTGVWVGNNKNEPMRGKADGSYVA
                     APIWNAFMKSAEANTTPKAFTSPKQIKTGKAVLDGYLPKTTVKIDKISGKIATDQTPP
                     DLIEEKSYYEAHSILWWVKKDDPRGEYPKNPYDDPATKNWEAGVQTWVKSKGGEFQLP
                     TEKDDLHTEKNIPNIEITSQKITPAKDSNNNTIPLEERKIDFTIKINAPLGVKKIEVL
                     LDGKSISIIDTNATTQSFSYNIKTLGEKEKYNFKIIVTDTAENKGETSVEISNL"
BASE COUNT         1184 a          720 c          643 g         1469 t
ORIGIN      
        1 ttcttcattg ccctttcttt ttctctttca aactctcgtt ttttaatttt ttcccgtttg
       61 tctattttct tttttgattt tgctaatcca atctctattt ttattttccc attcctatta
      121 taagccctta atggtactaa tgtcaagccc ctttgttttt gttttccaat caagctctta
      181 atttcgcttt tctttagtaa taattttcta gttcgtaagg ggtcataatc ctttggagta
      241 ttatttggct gatagggagg aatgtaagca ttgattaaaa atgcctccat atttttaata
      301 atagcaaaag aaccttttag ggaaaggcgt cctgttttta ttgatttaac ctcaaatcca
      361 tacaattcaa taccagcttc gtatttttca atgatttcat aatcaaaata agccctttta
      421 tttttagcaa aaatttttga cataatataa ttgcggatta gcaaatttaa tgcaaatgcg
      481 gcgagtttaa ccagcgcttt taaatttaat tcgttggctt atacaaaaag cgatgcgacc
      541 aaaaatttca ttcttttaac accttatatt cttacatttg ttgatgcaat tcgtttacta
      601 atatgttaaa atatttctat gtccaaatca ttaggtcaaa ttggtgaaaa tattgccgcc
      661 caatatcttc aaaagaaagg atatataatt ttagaacaaa actacaaaaa gccgtggggt
      721 gaaattgata ttattgtaaa aaagggaaaa gatattattt ttgttgaagt taaaactgtg
      781 agtaataata attttttacc agaagaagaa ataaccccaa gaaagaaaaa aattcttatc
      841 agaacagcta aactctatct tgcagaaaaa agatatccgg ctgatcaaga ttggcaaatt
      901 gatgttatag gcattgaaat gaaagataat aataaatgtt tattgaggca cacggaaaat
      961 gcgataggcg aaaactctaa actctaaata aacccaaaac tctaaattaa ataatgtact
     1021 atcgaggttg aacctcgata atggataatt aaaaaaaggt ttgaaaattg taaaggtcaa
     1081 agaggcttcg cctcttttca cgcctcactt cactttaaga agttcggcca ttaaaaattt
     1141 aatagaaatt aaaaattata aattagaaat ttctacgctt gtttctcctt tgttttctgc
     1201 tgtatcagta acaatgattt taaaattata tttttctttt tctcccaatg tttttatatt
     1261 gtaactaaag gattgggttg ttgcgtttgt atcaataatg cttatacttt ttccatcaag
     1321 taaaacctca atttttttta ctcccaaagg agcgttaatt ttaattgtaa aatcaatttt
     1381 tctttcctct aacggaattg tattattgtt gctatccttt gctggagtaa ttttttggga
     1441 agtaatttca atgttgggaa tatttttttc ggtgtggaga tcgtcttttt cagtaggtaa
     1501 ttgaaattca ccgcctttac tttttaccca agtctgaact cctgcctccc aattttttgt
     1561 tgctggatcg tcataaggat ttttgggata ttctcctcgt ggatcatctt tttttaccca
     1621 ccataaaatg gaatgagctt cataatagct tttttcttct atcagatcag gaggtgtttg
     1681 gtcagtggct attttccctg aaatcttatc aattttgact gttgtttttg gcaaataacc
     1741 gtcaagcact gctttgcctg tttttatctg cttgggagag gtaaatgcct tgggagtagt
     1801 atttgcttca gctgatttca taaaggcatt ccaaatcggc gcggcgacat aagatccatc
     1861 agctttaccc cgcatcggct cgtttttatt gtttcccacc cagacacctg taacaagatc
     1921 ttttgtataa ccaatagtcc aggcatcagt tatttttcct ttttctgttg atgcagtacc
     1981 tgtttttact gctgtccagg aattaagata aagtttagat cttgaaccaa acattggtgc
     2041 tcgtaaactg tcatcagata atattgaatt aatttgacgg caaacctcgc tgtcaagtgc
     2101 ttgaatagga tgtgattcgg gttcataaat tatctctcct cttgcatttt caatttttag
     2161 aatgtaattg ggcagatagc gaatgccctc agtagcaaaa acaccatagg cagatactaa
     2221 ttccaggggc attacatcct ttcctccgat agccatactc agattgggag aattcttatc
     2281 tagttctgaa tcaaaggtat caatcattcc catgcttttt gctgtgttta ttgtttcatt
     2341 aattcctgcc aaataaaggg ttttaactgc aggaatattt aaactttgag ctagggcttc
     2401 tttcattttt atcggtcctc gttgctttaa atcatagtta tctggccagt caggtttttt
     2461 ttcatcaaaa acagttttta catcccataa aatagtatca ggtgtatatc cctttttaaa
     2521 ggcagtagca taaacaatgg gcttaaaagc agaaccaggg gattggaatc catattccca
     2581 aatatttacc tggctttctt caaaattttt tcctccaacc attgctaaaa tttcaccgtt
     2641 atttggatta attgatacca gtgctacatt gccggcacca taccggtttt ctactgttgg
     2701 agcccaatca cttactgtgt tttctgctag ggactggaga tcaagatcaa tggtagtgaa
     2761 aacccgcaac cccatttggt catactcacc atacatttcc tcaagctgtt tttctacctc
     2821 acttgtaaaa tatggagctt ttctgggttg agacattgga cttaaagtga ttttttgttt
     2881 ttttgcttct tctgcttctt cttttgtggt aaatttcaat tcaaccattc gatcaagaac
     2941 ccaatttttt ctttcttctc tgtcctctgg gtttttgagg taataagtgg gtgattgagt
     3001 catagcagct aaggttgctg cttctgccag ggttagttca gaggccgatt tttggaaata
     3061 accttgactt gctgcttcgg ctccgtagaa aacagatcca taagaaattt gatttaaata
     3121 tgcttctaga atttgatctt tagaatattt tctttcagtc tcaagggcca ttattgcttc
     3181 ttgaatcttt cttgtaatgg ttttaggagc aggtccaaca atttctgttt tcccttcttc
     3241 atttgtttca gtctccaaaa aaacgacatt tttgataagt tgttgagtaa tggtagaacc
     3301 gccttgagtt gactttccta aatggagcac atcataaata atagctctta caattgcttt
     3361 aaaatcaata ccaggatgac tataaaaatc atcatcttcg gcagctagag ttgctttttg
     3421 cagtaaagga gaaatttgat ctaaactaac agaagtcttt tttttctcac ccgcttcata
     3481 taataaataa gaaccggtcc tgtcataaat tttagtagat tctccaatta ttctttcatc
     3541 gaggtcttcg atattcggag ttttgctggc aaaataagca aaaacaccta ttcccaccaa
     3601 aagcaaaaaa ataaaaacgc cacccacaat tttgggccat gaacggtgtt ttttatcttt
     3661 tttcctttta tgcttgtctt caatagtagt acgataatat ttacgaatag gcataacaat
     3721 agttcaaaat cgagtcttgc aacagcaaga cgagatgctc aaagaatttc attcttttta
     3781 cgtctcagct taagcttcaa cggaagaaga gaacaaagtt ctcttcgcct atcaaataaa
     3841 aatttataga tacttgtgcc ttcagcacga tattgataga tacttgctcg cttcgctcgc
     3901 gatattcatt gtaacaataa atatctataa atatctataa atatctataa atatctacaa
     3961 gtatctattg ttattttgca ttttcttata ttttgcctta aattttttaa tttgac
//
LOCUS       DAOTQD010000037         3686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_19924_length_3686_cov_8.549435, whole genome shotgun sequence.
ACCESSION   DAOTQD010000037 DAOTQD010000000
VERSION     DAOTQD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19924_length_3686_cov_8.549435"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..395
                     /locus_tag="P5570_02050"
     tRNA            319..395
                     /locus_tag="P5570_02050"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:His,seq:gtg)
     gene            402..1658
                     /gene="hisS"
                     /locus_tag="P5570_02055"
     CDS             402..1658
                     /gene="hisS"
                     /locus_tag="P5570_02055"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRR39517.1"
                     /translation="MEEIESKILKGFRDFLPQQMAIRQKIFDIVKNVFESYGFDAIET
                     PSLEYADILLGKYGEEADKLIYRFRDQGNRDVALRYDLTVPLSRVIAQYGNQLVKPFK
                     RYQIQPVWRADKPQKGRYREFTQCDVDIVGSSSPLADAEIIAVIYDILKKIGFEKFTI
                     RINSRQTLFSIIENAGVPTEKALLVIRSIDKLDKVSREEVEKELTIKTIEPEIIKKIF
                     SSIEKAEPDLFLIDVLSLIKKFGVPENFFKFDPTLARGLDYYTGPIYETIVEKPKIGS
                     ITGGGRYDKLIGMFTGKEVPACGTTIGIDRIIDVIDELNLWPELASTKNQILITLFDK
                     SFLDQAVEISQILRIQNINNEIYLNFGDRLEKQLKYADKKGIPFAIIFGPEEIQKDTI
                     ILKNLKTREQKEIRKSNFIETIKTII"
     gene            1655..2827
                     /locus_tag="P5570_02060"
     CDS             1655..2827
                     /locus_tag="P5570_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR39518.1"
                     /translation="MSVIKLTKQLVSIPSYLSSKTNEKIIGDFVFSYLKQFPYFKIKK
                     QKIDKKRFNIIAETSDVPRLFLAGHLDTVEPKQGWSKNQFKGITKNGKLYGLGSLDTK
                     SGVAAILDSLCDFKKISNLNLLFYCDEEYNFKGMKKFIASPKKKIGNLAILVEPTDLK
                     IWNTHRGLIEIYFSIQGKSGHAADPKSGKNAIDGITRAFVDLQNRLEKFTDPILGIPS
                     FNIAYIRGGLYQGNRNGNIKLGKEGNNIADFAEAVIDIRPTNQKLRVKKIKAWLEEFL
                     IKEGYKLLNFSVRHDFAPLYSDSSNPSELKILEKVLQKNLGKVGYLNPSKKGYGDGQL
                     LQEKFNIPVIYLGPKGANAHGIDEWVDINSLKKLRQIFSELIASYCKTEHHHNLPL"
     gene            3162..3560
                     /locus_tag="P5570_02065"
     CDS             3162..3560
                     /locus_tag="P5570_02065"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39519.1"
                     /translation="MNLNFEKIQKLSSIFSIIVLCLITLSASLTAYAQTYLPTGVTAP
                     PAPPVTEVNQIIKILNYIVNVIFTVVIIVAVIMFLYAAFTYITAGGDSSKTGQASKML
                     QWSAIGLGVALLALGIRSLIESFLQTGSNY"
BASE COUNT         1284 a          617 c          655 g         1130 t
ORIGIN      
        1 agcgcttgct ttacacgcaa gaggcctgag gttcgagccc tcaatcgccc accagcacct
       61 gactttccga tttttcagaa accatttttt gccccgcgag ccgaaattta tcgcgtgcca
      121 caccaaaatt tccaaactat tgactttctt ttaggatctg ctaccataat aaagtatgat
      181 gcccagtaaa ggtaaaaaca taataaatat tcatcataat catatcctct tgtttccgaa
      241 ggggcaggtt ttattttgat tagatcgtaa tttttaaaga cttcctgacc tcttctagca
      301 agaggttttt ttaatctggt ggacgtagct cagttggcta gagcgcttcg ctgtgaacga
      361 agaggtcgcg ggttcgaatc ccgtcgttca ccccaaaact tatggaagaa atcgaatcaa
      421 aaattttaaa aggatttcgt gattttttac ctcaacagat ggcaattcgc caaaagattt
      481 ttgatattgt aaaaaatgtt tttgaatcct atggttttga tgcaatcgaa acgccctcat
      541 tagaatatgc ggatatatta ctaggaaaat atggagaaga agcagataaa ttaatttatc
      601 gttttagaga tcaaggcaat cgagatgtgg cccttcgtta tgatctgaca gttccattat
      661 cacgagttat cgctcaatat ggcaatcaac tcgtaaaacc ttttaaacgc tatcaaattc
      721 aaccagtctg gagagctgac aaaccacaaa aaggacgtta tcgagagttt actcaatgcg
      781 atgtggatat agtaggcagt agttcgccat tagcagacgc cgaaattata gcggttattt
      841 atgatattct gaaaaaaatt ggttttgaaa aatttacaat tcgcattaat agtcgccaaa
      901 ctcttttttc tatcatagag aatgctggcg ttcctacgga aaaagccctt ttagttattc
      961 gatctattga caaattagac aaagtttccc gcgaagaagt agaaaaagaa cttaccataa
     1021 aaaccattga accagagatt ataaaaaaga tcttctcgtc tattgaaaaa gcggaaccgg
     1081 atttgttttt gatagatgta ttatcattaa taaagaaatt cggagttcca gaaaatttct
     1141 ttaaatttga tccgactctg gctcgaggcc tcgactatta cactggtcca atttatgaaa
     1201 caatagttga aaagccaaaa atcggttcaa ttaccggtgg cgggagatat gataaattaa
     1261 ttgggatgtt taccgggaaa gaagttcctg cctgtggaac aacaattgga atagatagaa
     1321 taattgacgt gattgatgag ctaaatcttt ggccagagct tgcttcaaca aaaaatcaaa
     1381 ttcttatcac tttgtttgat aaatcttttt tggatcaggc agtcgaaatc tcccaaatac
     1441 ttcgtatcca aaacattaac aacgaaattt atcttaattt tggggatcga ttagaaaaac
     1501 aacttaaata cgccgataaa aaaggaattc cttttgcaat tatatttgga ccagaagaaa
     1561 tccagaaaga tacaattatc ttaaaaaatc ttaaaacaag agagcaaaaa gaaattcgaa
     1621 aatcgaattt tattgaaacg ataaaaacaa taatatgagc gttattaaac ttacaaaaca
     1681 acttgtttcc ataccaagct atctcagttc aaaaactaat gaaaaaataa ttggtgattt
     1741 tgtattttct tatctcaagc aatttcctta ttttaaaatt aaaaagcaaa aaatagataa
     1801 aaaacgtttt aatattattg cagaaacctc cgacgttccc cgtttatttt tggccggcca
     1861 cttagatacc gtagaaccaa aacaaggttg gtccaaaaat cagttcaagg ggataactaa
     1921 aaatgggaaa ttatatggat taggctccct tgatacaaaa agtggagtag cggcaattct
     1981 tgattctctt tgcgatttta aaaaaatatc taatctcaat cttctttttt attgcgacga
     2041 agaatacaat tttaaaggta tgaaaaaatt tattgccagt cctaagaaaa agattggaaa
     2101 tctcgctatt cttgtcgaac caactgatct caaaatttgg aatactcata gaggtcttat
     2161 tgaaatttat ttttctatcc aaggaaagtc tggacacgca gcggatccca aatcagggaa
     2221 aaatgccatt gacgggataa cgagagcatt tgtcgatcta caaaatagac ttgaaaaatt
     2281 taccgatcca attcttggca ttccatcttt caatatcgct tatattcgtg gcggacttta
     2341 ccaaggaaat agaaatggaa atataaaact tgggaaagaa ggaaataata ttgccgattt
     2401 tgctgaggcg gtaattgata ttcggcccac taatcagaaa cttcgagtta aaaaaataaa
     2461 agcctggctt gaggaatttt tgataaaaga aggatataaa cttctcaact tttcagttcg
     2521 tcacgatttt gctccgctct acagtgattc aagtaatcca tcggaactaa aaattttaga
     2581 aaaggttctg caaaaaaatc taggcaaagt tggttatctc aacccatcta aaaagggata
     2641 cggcgatggc cagctattgc aagaaaaatt taatattcca gtaatttatc ttggtccaaa
     2701 aggtgcaaat gctcacggga tagatgaatg ggtagacata aactctctta aaaaattaag
     2761 gcaaatcttt tcggaattaa ttgcctctta ttgtaaaacc gaacatcatc acaatcttcc
     2821 cttgtaggaa aggactggtt gtgtttgatt tttctaaact ttttattaaa caccctcaac
     2881 cccttctcag aaaaggcttt ctttttgaga aggattttta ttctttaaaa aattaaagga
     2941 tgaaaaacaa aatgggagta gaagtcgaaa agttagacgt gaaaaatgag aaagtcttaa
     3001 ggaagtgcgc ggaactttat tgccaaattc aggaattttt aataaaataa gctcgagcaa
     3061 agttgcataa atacgccatg aaaaatatct tgacaattaa aatttttact tatacaataa
     3121 attaatatta atagcggtcg aaacaattta atttaaaaaa tatgaattta aatttcgaaa
     3181 aaattcaaaa actaagctca attttttcaa taatagtttt gtgcttaatt actctttctg
     3241 ctagtttaac agcttatgct caaacttatt taccaaccgg ggttacagct cctccagctc
     3301 ctcctgtaac agaggtaaat caaattataa agattctaaa ttacatagtt aatgttattt
     3361 ttacagtagt aattattgta gcagttataa tgtttttgta tgcagctttt acttatataa
     3421 ctgctggggg agattctagt aaaaccggcc aggctagtaa aatgcttcag tggtcagcaa
     3481 ttggtttggg ggttgccctt cttgctttag ggataagaag cctgattgaa agctttcttc
     3541 aaacagggag caattattaa ttttattcgt attgatttaa acaaagcaag cttctaggct
     3601 tgctttgttt tttgaaaaat aataaaatct gaaaaagtcg gagtggcgga attggcagac
     3661 gcgtcaggtt taggacctga tggatt
//
LOCUS       DAOTQD010000038         3600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_20528_length_3600_cov_5.099577, whole genome shotgun sequence.
ACCESSION   DAOTQD010000038 DAOTQD010000000
VERSION     DAOTQD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20528_length_3600_cov_5.099577"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1620)
                     /locus_tag="P5570_02070"
     CDS             complement(151..1620)
                     /locus_tag="P5570_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRR39520.1"
                     /translation="MFFKAKNIFIVFAVLLGLVFSYGKVLAAPSDKYDELKIQLDSLT
                     QEMNLCTALPSGQEKIDCINSVIKKMEEVNVELDKYAKEIGARADELQKDIKSLSNQI
                     GYLDAKVEKTEVEIRITQQEIDMLNLDIEKIEEEIKLTEEEIKENENKIEEVRQRLAD
                     TVKKIYEYDAQNLIKITLTEGTLSDFFDEISYVESLQKGLADNLAKLKENKSNLEAKK
                     ATLEDQKASLAEKRREVSEKIDSFNKTIAELDAEKKNKATLLEITKGDEEEYQKLLAE
                     IEEQKKQLLGNLSALSQSRQAEIEEIVQKTGGRIDPIVFGNYTYFAQDSGPWAQTCIH
                     NICPPIGDTMATSGCFITSMAMLLKSYGKEVTPATLATDDTILEGIFLNASAAASKYA
                     LKCLDGCSSSTWGNFNKNTVNSYLDASKPVILGIQTSGGGTHFVLAIGRIGDNYYVLD
                     PIYNVSQGKAVNVQASIDYINSLPMYSGAKVSRMVLIDY"
     gene            complement(1633..3144)
                     /gene="gltX"
                     /locus_tag="P5570_02075"
     CDS             complement(1633..3144)
                     /gene="gltX"
                     /locus_tag="P5570_02075"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRR39521.1"
                     /translation="MSKLPLKKSDTIRVRIAPSPTGPLHIGTARTALFNYLFARKNQG
                     NFILRIEDTDLERSDPKWEKDVVENLKWLGILWDEGPDIGGKYKPYRQSERKDIYEKY
                     IKKLYDEGYLYWCFCTKEELEAQREDQMARGEAPRYMGRCRNLSLKEQEKFRQEGRKG
                     ILRFKVPEKIIKVNDLIRGKLEFNTSLLGDIAVAKDFSIPLYNFAVVIDDFEMKINYV
                     IRGEDHISNIPKQVLFQEALGFPRPNYAHLPLILGPDRSKLSKRHGAVKVQDYKEDGY
                     LPEALINFMALLGWNPGTKEEIFSLEELTKKFSLERVQKGGAIFNIERLNWINGYYIR
                     RIPLAELTEKCIPYLIKSQISKDKSQNKFQIKATGEIIDFNYLKNIVALFQERLKKLS
                     EISELADFFFKDKLEYDKSLLFWQKITEKDLQKNLKKCYKILKKLKENEWQREKIKEV
                     LLKEAEKEINRGYILWPLRVSLTGKKASPPPFEVAEILGKEKTLKRIEEALQK"
BASE COUNT         1033 a          696 c          498 g         1373 t
ORIGIN      
        1 gcccaaaaag aaaaggtcat aattacaaac gattgttgta gattatgacc cttttcctgc
       61 ttctattaaa tttgttttta tccaagaagt gggtttaaaa tattaaaaaa caacatatct
      121 tctacgaaat taaaattacg aataaaatgt ttaataatcg ataagaacca ttctcgaaac
      181 ttttgctcca ctatacattg gaagactatt tatataatct atactagctt gcacatttac
      241 tgcttttccc tggcttacat tatatatggg atctaataca tagtaattat cacctattct
      301 tcctattgct aaaacaaaat gagttccacc accagaagtc tgaattccaa ggattaccgg
      361 cttactcgcg tcaagatatg aatttacagt gttcttatta aaatttcccc aagtcgaact
      421 actacatcca tcaaggcatt tgagggcata tttactagcc gcagcagaag cgttaagaaa
      481 aattccttca agtattgtat cgtcagtcgc caatgttgcc ggagtaactt ccttaccata
      541 tgattttaat aacattgcca tcgatgtaat aaaacatcca gatgtcgcca tagtgtctcc
      601 tatggggggg caaatattat gtatacaagt ttgtgcccac ggtccactat cttgagcaaa
      661 atatgtataa tttccaaaga cgataggatc tattctacct cctgtttttt gaactatttc
      721 ttcaatctct gcttgtcttg attgtgacaa agctgataaa tttcctaata attgtttttt
      781 ttgttcttca atctctgcta acaatttttg gtattcttct tcatctcctt ttgtaatttc
      841 aagtaaggtt gctttattct ttttttcagc atctagttcg gcaatggttt tgttaaaact
      901 atcaattttt tctgaaactt cacgtctttt ttccgctaaa cttgcttttt gatcttcaag
      961 tgttgctttt tttgcttcta gattactttt attttctttt aattttgcaa gattatcggc
     1021 taatcctttt tgaagacttt caacataact tatttcatcg aaaaagtcag aaagagtacc
     1081 ttcggttaat gttattttta tcaaattttg agcatcgtat tcgtaaatct tttttactgt
     1141 atctgctaat cgttgtctta cttcttctat tttattttca ttttctttaa tttcctcttc
     1201 tgttaattta atttcctctt caatcttttc aatatcaagg ttaagcatat caatttcttg
     1261 ctgggtaatt cttatttcaa cttctgtttt ttcaacttta gcatcaagat atccaatttg
     1321 attacttaaa cttttaatat ctttttgaag ttcatctgct ctagcgccta tttctttggc
     1381 atatttatca agttctacat taacttcctc catctttttg attactgaat taatacaatc
     1441 aatcttttct tgtccagatg gtaaagcagt acagagattc atttcttgtg ttaaagagtc
     1501 aagttgaatt ttaagttcgt cgtatttatc tgaaggggct gctaaaacct ttccataact
     1561 aaaaaccaat cccaaaagga ccgcaaaaac tataaaaata tttttggctt taaaaaacat
     1621 aaaataaaaa tttcattttt gtaacgcttc ttctattctt tttagcgttt tttctttgcc
     1681 tagaatttca gcaacctcaa agggtggagg cgatgccttt tttcctgtta aagaaacccg
     1741 caaaggccaa aggatatatc ccctattaat ttctttttct gcttctttta gcaaaacttc
     1801 tttaatcttt tccctttgcc attcattttc ctttaatttc tttaaaattt tataacattt
     1861 ttttaaattt ttttgaagat ctttttcagt tattttttgc caaaaaagca aacttttatc
     1921 atattctaat ttatccttaa agaaaaagtc agccagttcg ctaatctctg atagtttttt
     1981 taatctttct tgaaaaagag caacaatatt ttttaaataa ttaaaatcaa taatttcacc
     2041 agttgcttta atctgaaatt tattttgaga tttgtctttt gagatttgag attttatcaa
     2101 gtatggaatg catttttcag ttagctctgc caatggtatc cttctgatat aataaccatt
     2161 tatccagttt aatctttcaa tattaaaaat cgctcctcct ttttgaacac gctccaaaga
     2221 gaatttttta gttaattctt ctagagaaaa aatttcctct tttgtccctg gattccagcc
     2281 caaaagagcc ataaaattaa tcagtgcttc tggcagataa ccgtcttctt tgtagtcctg
     2341 gacctttact gcaccatggc gctttgaaag ttttgatctg tctggtccta aaattaatgg
     2401 taaatgagca taatttggcc gaggaaatcc aagggcctcc tgaaacaaca cttgttttgg
     2461 aatattagaa atgtgatctt ctcccctaat cacataatta attttcattt caaagtcatc
     2521 aatcacaact gcaaaattgt aaagcggaat tgaaaaatcc tttgctacag caatatcgcc
     2581 aagtagcgaa gtattaaatt ccaattttcc cctaataaga tcattcactt ttatgatttt
     2641 ttccggtacc ttaaaacgca aaataccttt tctgccttcc tgacgaaatt tttcttgttc
     2701 ttttaaagaa agatttctgc aacgacccat ataccgtggt gcttctcctc gagccatttg
     2761 atcctctctt tgggcttcta gttcttcttt agtgcaaaaa caccaataca aatatccctc
     2821 atcgtataat tttttaatat atttttcgta aatatctttt ctttcagatt ggcgatatgg
     2881 tttatattta cctccaatat ctggaccttc atcccataaa attcccagcc attttaaatt
     2941 ttcaacaaca tctttttccc attttggatc agatctttca aggtcggtat cttcaattct
     3001 taaaatgaaa ttcccctgat ttttacgggc aaaaagataa ttaaaaagcg cagtccttgc
     3061 agtgccaata tgtaatggtc cggttggaga aggcgcaatt cttactctta ttgtgtctga
     3121 tttttttaaa ggcaatttag acataaaaat attaaattaa aactaagttt taaaattttt
     3181 atatttgata gattcaattt tttaacgcaa ccatacatcg agatcgccac gtcgctcgtt
     3241 tcactcgctc ctcgcgatga cacttttcgt tctttgtctt tgacacgagc gcagcgcagc
     3301 gcctccgcca ctgtctttgc gagaagtgaa cgaagtgaac gacgaagcaa tcccgggcgc
     3361 agcccgcaac catgcaacgg gatcgcttcg tcatccactc accttttact aaggtgaggg
     3421 actcctcgcg atgacaagaa acgtgccatg agattgcttt tctttctgtt ttctgttttc
     3481 tgacaactga tttctgtttg gtgttttatt atttgtaaat attgcattaa atttttgaac
     3541 ctaactttat tatatttctt ctaagataac tgttgcaact tttttcgctg catcgggcat
//
LOCUS       DAOTQD010000039         3596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_20558_length_3596_cov_9.584016, whole genome shotgun sequence.
ACCESSION   DAOTQD010000039 DAOTQD010000000
VERSION     DAOTQD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20558_length_3596_cov_9.584016"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(500..787)
                     /gene="gatC"
                     /locus_tag="P5570_02080"
     CDS             complement(500..787)
                     /gene="gatC"
                     /locus_tag="P5570_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRR39522.1"
                     /translation="MSIDHILKLARLEISEEEKNNLEKEFSSILDFVKKIEEIKINEK
                     EFSVINKSKGIKNVMREDKQEISNSQFIISKKLIASALEKKDRYIKTKQIL"
     gene            complement(825..1859)
                     /locus_tag="P5570_02085"
     CDS             complement(825..1859)
                     /locus_tag="P5570_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRR39523.1"
                     /translation="MFFHRDIGIDLGTANTLVYVRGKGIVINEPSVVAINKKTGQVLA
                     VGEEARKMVGKTPAHIIATKPLIGGVVSDFEITEQMLRYFIDKVFGKRFFPWPQPRLV
                     IGIPSGVTEVERKAVEDAAKNAGAGEVYLVEEPMAAAIGSRLPVQEAGGNMIVDIGGG
                     TTEVAVISLGGIVNSKSIRTAGNRLDQDIVRFAEEEFKLVLGERTAEEIKIKIGSAMP
                     LKEELQYPMRGRDIITGLPKEVVVTDADIREAIKTSIRTIVSEVKAAVEETPPELLAD
                     IMARGIYLVGGGSLLRKLDELLQKETKIPVKVPEDSMTAVARGCGIILEDLPKLREVL
                     VSSEELEAPK"
     gene            complement(1916..3190)
                     /locus_tag="P5570_02090"
     CDS             complement(1916..3190)
                     /locus_tag="P5570_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRR39524.1"
                     /translation="MFFSQIFLKTRKDIPKGAEVASHKLLLKAGFVDQISSGSFCFLP
                     LGWRVHQKIANIIREEMNKIGGQEVYFNALIPKELWEKTGRWETMDPPLFRVKDRHKQ
                     EFGLGSTHEEVVTKIAKSYIQSYKDLPIALYQIQNKFRNEMRFNGGLLRTREFTMKDL
                     YSFHQDEKDLEKFFDKVLLAYEKIFERCGLNAIKSEASGAGFAEKGAKTYEFQVENEI
                     GEDRVIFCPDCKWAANSEISPKKEGEHCPRCKGSLIQIKTIEVGHTFMLGTKYAKALN
                     LYFKDKKGKENLVFMGCYGIGLGRLMATIVEENYDEKGIIFPKEIAPFDVCLIPFESQ
                     NSKIKSQIKKTADSIYCTLQKQGFELLYDDRENKSAGEKFADCDLIGIPQRIVISEKT
                     LAKECVEIKGRKEKKGKLMKINQIYLNQKSKL"
BASE COUNT         1114 a          772 c          469 g         1241 t
ORIGIN      
        1 cagcgaagcg ttgaaaagaa gacacatctt ctttgagttc gggcgaagcc cgcaacagca
       61 ctctgggatt gccacgtcct actgcggtag gactcgcaat gacactcttt attctttgtc
      121 tttgaggaca cgagccgcag cgatcccgtt ccccttctgt cattgcgagc gcagcgaagc
      181 aatctcgagt gaacagggtg agctcgttga aaagaagacg catcttcttt gagctcggac
      241 gaagcccgca ataatgtcac gggatcgcca ccccctcacc tttgtgaagg tgaggggtcg
      301 cgatgacaaa tatagttatt actccctgaa tcacattgaa aggttacctt tgtaaaaatt
      361 ccaaatacca aattacaaat tctaaacaaa ttcaaaatac taaatactaa acagaaatca
      421 gaaaaaggaa atcagaaatg agacaatccc tttctaccct cctctacctt ctaccctcta
      481 ccttctaccc tctatttaat tatagtattt gttttgtttt aatatatctg tcttttttct
      541 ccagcgctga tgcaattaat tttttggaaa ttataaattg agaattagaa atttcttgtt
      601 tatcttctct cattacattt tttattccct tgcttttatt tataacagag aattcctttt
      661 cattaatttt tatttcctct atttttttga caaaatccaa aatagaagaa aattcttttt
      721 ctaagttgtt tttttcctct tctgaaattt ctaatcgtgc taattttaga atatgatcaa
      781 tagacatagt ttgactttta aggaattagc ttgtagctta ttagctactt cggtgcttct
      841 aattcttcac ttgaaaccaa aacctctctt aattttggta aatcctctaa aataatacca
      901 cacccgcgag ctacagctgt cattgaatcc tcaggcactt tgacaggtat ttttgtttcc
      961 ttttgtaaaa gttcgtctaa ttttcgcaaa agactgcctc ctcctactag ataaattcct
     1021 cgtgccataa tatcagctaa tagttctggt ggcgtttcct caactgctgc cttcacttca
     1081 ctaacaatag tccgaattga agtttttatt gcttctctta tatctgcatc tgtgacaact
     1141 acttctttgg gaagaccagt aataatatct ctgccccgca ttggatattg caattcttcc
     1201 tttaggggca ttgcggaacc aatttttatt ttaatctcct cagctgttct ttcacccaat
     1261 actagtttaa attcttcttc ggcaaaacga acaatatcct gatctaacct atttccagct
     1321 gtccttattg attttgaatt tactattcca cccaaagaaa ttaccgctac ctcagtagtt
     1381 ccgccaccaa tatcaacaat catattgcct ccagcttctt gaaccggcag acgagatcca
     1441 atagctgctg ccattggctc ttctaccaga tacacctcac ccgctccagc gttttttgcc
     1501 gcatcttcta ctgcctttct ttctacttca gtaacgccag atggaattcc aattaccaaa
     1561 cgaggttgtg gccagggaaa aaatctttta ccaaacacct tatcaataaa ataacgcaac
     1621 atttgttcag taatttcaaa atcagaaacc actccaccta ttaggggctt tgtagctata
     1681 atgtgagcag gtgttttgcc caccattttt ctggcttctt ctcctacggc caaaacctga
     1741 ccggtctttt tattaatggc tacaaccgac ggttcattaa tcacaattcc ctttcccctt
     1801 acataaacta aagtattggc tgtaccaaga tcaatgccta tatctcgatg aaaaaacata
     1861 atataaaaac gcaaatatca aaattaaaat atatcttttt attcctttga attttttata
     1921 attttgattt ttgatttaaa taaatttgat ttattttcat tagtttccct ttcttttctt
     1981 ttcttccctt tatttcaaca cattctttcg ccaaggtttt ttcagaaatt acaatccttt
     2041 gaggaatacc tattaaatca caatcagcga acttttcccc tgctgattta ttttctctgt
     2101 catcatacaa taactcaaat ccctgttttt gtaaagtaca ataaatacta tcagcagttt
     2161 ttttaatttg agattttatc tttgaatttt gagattcaaa aggaataagg caaacatcaa
     2221 atggcgcgat ttcttttgga aaaataatcc ccttttcatc ataattttct tcaacaatgg
     2281 ttgccattaa tcgacccaac cctatcccat aacaacccat aaaaaccaaa ttctctttac
     2341 cttttttatc tttaaaataa agattaaggg ctttggcata ttttgttccc aacataaaag
     2401 tatggcctac ttcgattgtt tttatctgta ttaaactgcc tttacatctt ggacaatgtt
     2461 ctccttcttt ctttgggctt atttcactat ttgctgccca cttacagtca ggacaaaaaa
     2521 tcaccctatc ttctcctatt tcattttcaa cctgaaactc atatgtcttt gccccttttt
     2581 cagcaaatcc tgctccagac gcttcgctct ttatggcatt taaaccgcaa cgttcaaaaa
     2641 ttttttcata agccagcagt actttatcaa aaaacttttc cagatctttt tcatcttgat
     2701 gaaaactata aagatctttc attgtaaatt cccgagttct taaaagacca ccattaaatc
     2761 tcatttcgtt tctaaactta ttttgaatct gataaagagc aattggcaaa tctttatatg
     2821 attgaatata ggactttgca attttggtaa caacctcctc gtgtgttgat cccaaaccaa
     2881 attcctgctt gtgacgatct tttactcgaa aaagaggcgg atccattgtt tcccaccgac
     2941 cagttttttc ccaaagctct tttggaatta aagcattaaa ataaacttct tgacctccga
     3001 ttttattcat ttcttccctt ataatatttg caattttttg atgaactctc caaccaagag
     3061 gtaagaagca aaaactgcca gatgaaatct gatcaacaaa accagctttt aataataatt
     3121 tatgagaagc gacttcagcc cctttgggta tatcttttct tgtttttaaa aaaatttgcg
     3181 aaaaaaacat attaacaacc ttataactaa tcattaaaat atttattttt attatagcaa
     3241 atttttgaaa tttttgaaaa ttaaaaaata ccaaagtaaa aattccaaag taaaaacaaa
     3301 ctccaaattc caaattccaa acaaattcta aattctaaat actaaacaat attaaacaga
     3361 aatcagttgt cagaaatcag aaaacagtag agaaatcaga aaacagaaat tattattaca
     3421 taaatttcca agctccaata accaatgacc aatcaaattc taatgttcaa aattctaaac
     3481 aaaagtgtac aacaccattg aacattagga tttagagctt gattgaaaat tggaatttgt
     3541 gatttggaat ttaatatttt atacgcaacg ccaacgttta ccgtatcacg ttaaac
//
LOCUS       DAOTQD010000040         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_21201_length_3502_cov_7.570351, whole genome shotgun sequence.
ACCESSION   DAOTQD010000040 DAOTQD010000000
VERSION     DAOTQD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21201_length_3502_cov_7.570351"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..520
                     /gene="rpmG"
                     /locus_tag="P5570_02095"
     CDS             356..520
                     /gene="rpmG"
                     /locus_tag="P5570_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011077215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRR39525.1"
                     /translation="MAKKKRKLIKMQCQECKKVNYYTYKNPKSVERKLEFKKHCKQCK
                     KHTPHKEAKK"
     gene            621..695
                     /locus_tag="P5570_02100"
     tRNA            621..695
                     /locus_tag="P5570_02100"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:654..656,aa:Trp,seq:cca)
     gene            860..1606
                     /gene="uppS"
                     /locus_tag="P5570_02105"
     CDS             860..1606
                     /gene="uppS"
                     /locus_tag="P5570_02105"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRR39526.1"
                     /translation="MENDIPQHIALIPDGNRRWARKRGFKPWIGHRAGVKSLENVLEK
                     ALDLKIPYFTFWGASFDNLTKRPEIEISFLNEIYTEQFKRVLKDKRIHQNEVKVNVFG
                     RWKEVLPSETQKAIEKAVEATKNYSKNFLTFLLAYNGTDEIKIAIQKIIDKAQKELLN
                     FQSPKKKNELKVTDELIKENLWTKDLPSVDIVIRTGCEGDPHMSAGFMMWDTAYSQYY
                     FTETLFPDFDSEEFEKVIKDFSQRERRMGK"
     gene            1811..2665
                     /locus_tag="P5570_02110"
     CDS             1811..2665
                     /locus_tag="P5570_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39527.1"
                     /translation="MKNILIADLDGTLVFKDQKISQEVGRYLLEIMKESWLIIIAAAS
                     AERVTNLFLEPLWNLGAKEEIENHLLLFSEMGLLEIDPKKAIDNLEDSFIVHLPDGHL
                     RRLKTLREEISKMIWVSPSLWHPLLPQNGGSLFKDYKGRTILLSKESDSPLFPYWILN
                     EEKRVLTTLEKIRSLEGKIIEAPKEVKTSILSFLQNGEVDAIEFPTSFEVKPKGWDKS
                     IPAAMALERIGAQKKCGLNTVVLGDTEHDRAMACPKGLGVNLPFIEIKGEEHLLEVLR
                     AIHRGTII"
     gene            complement(2679..>3502)
                     /locus_tag="P5570_02115"
     CDS             complement(2679..>3502)
                     /locus_tag="P5570_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRR39528.1"
                     /translation="SGKIVTQQIINLINFLINEEKSSKNDFFGIGISSIGPLDYQKGG
                     PLNAPNVPFKFIPLTTPLKNHFSLPVFLLNDCNAAVLGEKYFGDGKNIENLIYITIST
                     GIGGGAIINEKLLLGKSGNAAEVGHFIVDTKYDLPCTCGKGKGHWEGYCSGRNLPRFL
                     KLWAKKEKRAVNFKVKESKDIFEAAKRKNKTALMFLDEVSKINAAAVSNIIVAYDPTL
                     ITIGGSVVLSNQEIILKGIKKYVDHYLKVPKIKITRLGKEITLLGAAAAVFWPIK"
BASE COUNT         1217 a          556 c          598 g         1131 t
ORIGIN      
        1 cttattataa taatcgaaac cctactcata aattaaacct caattagatt ataaccattt
       61 ttaattaaaa aataaataga aacttatctg ccatcaaaat ataggttaga ttcaagaatt
      121 taatgcaaca ctctttatta atttttaatt tttaattttt aaacccttaa atccgtattc
      181 gtgaaataga atgcgtctta cggtaaacgt tggcgttgcg cgtatcgtct tacggtagag
      241 aagtgcgtaa agtatacgct tgccgtttta cgcttgccgc gacgcgctac gctaccgttt
      301 tacgaaattg tgttaaattt ttaaacctaa attatataat ataaatttaa aaaatatggc
      361 aaaaaaaaag agaaaattaa taaaaatgca atgtcaagaa tgtaaaaaag ttaattatta
      421 tacttacaaa aacccaaaat cagtggagcg gaaattagaa tttaaaaaac attgcaaaca
      481 gtgtaaaaag catacaccgc acaaagaagc aaaaaaatga tagaaaggaa atcagatttg
      541 cttattttgg tcctgcccag ctagaacttt tttgagtttt ccctgaaaat tatttaaatt
      601 aagatagaaa aaattcattt gggggcgtgg tgaaatggct atcatgggaa tctccaaaat
      661 tctcgttcta ggttcgagtc ctagcgcccc tgccacaaca aaattataaa gaatttttgt
      721 atgattgtgg cgtttaagat tacaaagcaa attttattac attacgccaa tttttagttt
      781 ttaaatttaa caaaacaact tgacaaatca caataaaaaa aattaaaata gtacttatta
      841 ttaaataatt tgttttttta tggagaatga tattccacaa cacatagcat taattcctga
      901 cggtaatcgt cgttgggcaa gaaaacgagg ttttaaaccc tggataggtc atagagcagg
      961 tgttaaatct cttgaaaatg ttttagaaaa agctttggat cttaaaattc cctattttac
     1021 tttttgggga gcgtcttttg ataatttaac caaacgtcca gagatagaaa tttctttttt
     1081 aaatgaaata tatacagaac agtttaaacg agttttaaaa gataaaagga ttcatcaaaa
     1141 cgaggtcaag gtaaacgtct ttggacgttg gaaagaagtt ttaccatcag aaactcaaaa
     1201 agcaatagaa aaagctgttg aagcgacaaa aaattatagt aagaattttt taacatttct
     1261 tttggcttac aatgggactg atgaaataaa aatagccatt caaaaaatta ttgataaggc
     1321 gcaaaaagaa cttttaaatt tccaatctcc gaaaaagaaa aacgaattaa aagtaacaga
     1381 tgaattgata aaagaaaatc tttggactaa agatttgccc tctgttgata ttgtgatcag
     1441 aaccggttgt gaaggagatc ctcatatgtc agccggtttt atgatgtggg acactgctta
     1501 ttcccaatat tattttacag aaaccctttt tccagatttt gattcagaag aatttgaaaa
     1561 agtaattaaa gatttttctc aaagagaaag aagaatggga aaataaaaaa aataaaatag
     1621 agttaattag gcaataaata aaattgatct aattaacaaa agagagaatt ttaaaagtac
     1681 cctctctttt tgtttttgtt ttcaaaatta aaataaagat aaaaggagag ggtattttat
     1741 atctttataa aatgcccttt ctctttcttt aaaagaaaga gttttgcaac ttaaaaatta
     1801 ggtgattggg atgaaaaaca ttttaattgc cgatttggac ggaactttgg tcttcaagga
     1861 ccaaaagatt tctcaagagg tagggagata tctcctggag attatgaagg aaagctggct
     1921 gatcatcatt gctgcggcaa gcgcagagag agtaaccaat cttttcttag aacctctttg
     1981 gaatttgggt gcaaaagaag agattgaaaa ccacctgctt ttgttttcag aaatgggatt
     2041 acttgaaata gatcccaaaa aagctattga taaccttgaa gattctttta ttgttcatct
     2101 acccgatggc catcttcgcc gtctgaaaac cctcagggag gagatttcaa agatgatttg
     2161 ggtttctcct tctttatggc acccgttact tccccaaaac ggtggtagtc tttttaaaga
     2221 ctacaaggga agaaccattt tactttccaa agagagtgac tctccgttat ttccttactg
     2281 gattcttaac gaagagaaaa gggtacttac cacccttgaa aagattaggt ctttagaggg
     2341 gaagataata gaagcaccca aggaagtaaa aacaagtatt ctttcttttt tgcaaaatgg
     2401 tgaagtagac gccatagagt ttcctacttc ctttgaggtg aagcccaaag ggtgggataa
     2461 aagtattcct gccgctatgg ctttggagag gatcggtgcc caaaagaaat gtggtttaaa
     2521 cacggttgtt ctcggtgata ccgagcatga tcgagcaatg gcttgtccca aaggtttagg
     2581 ggtgaatctt ccctttattg agataaaggg tgaggagcat ctcttggagg tacttcgagc
     2641 cattcacagg ggaactatta tttaattatt agttcccttt attttattgg ccaaaagaca
     2701 gccgctgctg ctcctaataa agtaatttct tttcccagtc gagtgatttt tatttttgga
     2761 acttttaaat aatggtcaac gtatttttta atacctttca aaataatttc ttgattactc
     2821 aaaactactg atccgccgat tgtaattaac gttggatcat aagcaacaat aatatttgaa
     2881 acagctgcag cgttaatttt agaaacctcg tcaagaaaca taagggctgt tttgtttttt
     2941 cttttagctg cctcaaaaat atctttactt tcctttactt taaaattaac tgccctcttt
     3001 tctttctttg cccaaagttt taaaaatcgt ggcaggtttc ttccagagca atatccttcc
     3061 caatgccctt tcccttttcc acaagtacat ggtaaatcat attttgtatc cacaataaaa
     3121 tgaccaactt ctgctgcatt gccacttttt cctaaaagca atttttcatt tataatagct
     3181 ccgccgccaa tgccagtaga aatagtgatg tagattaaat tttcaatatt tttaccatca
     3241 ccaaaatatt tttctcccaa aaccgctgca ttgcagtcat tgagtaaaaa aactggtaaa
     3301 gaaaaatgat ttttaagtgg tgttgtcagg ggaataaatt taaatggaac atttggagcg
     3361 ttaagcggtc ctcctttttg ataatctaat ggtccaatag atgaaattcc gattccaaaa
     3421 aaatcattct tacttgattt ttcttcattg attaaaaaat taatcaaatt tattatttgt
     3481 tgagttacaa tttttcctga tt
//
LOCUS       DAOTQD010000041         3329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_22582_length_3329_cov_8.487783, whole genome shotgun sequence.
ACCESSION   DAOTQD010000041 DAOTQD010000000
VERSION     DAOTQD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22582_length_3329_cov_8.487783"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="P5570_02120"
     CDS             complement(<1..349)
                     /locus_tag="P5570_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39529.1"
                     /translation="MIQDEIERLRRARIRLEAAFKTKIVLEESVEEALRQEVDLLEKV
                     VEKVENKTGDKNGQTESQLKETPEQQGEQQQGEQQQGEQQEPAREEETSQQVEDEEQT
                     EEQTGGQTQPVSEK"
     gene            complement(380..1447)
                     /locus_tag="P5570_02125"
     CDS             complement(380..1447)
                     /locus_tag="P5570_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR39530.1"
                     /translation="MNIYYVLAPRNLFVTIVNESQCKARMKAGKFVDFLMPREGCGFD
                     KNWNIVSCPNKRKRALGGLYWIGIPPFWEIYKYKFSWTGIDVNGEVVSHRHEILDYVL
                     IVDDVYWASIDKAEDKNLVPLDIEILVTMRVINPYKALFHVQDWSEMVMNRIKPLIRE
                     YVSAFEFEEIVKKKQEKGGEIYGKLKGTGLIKEFESSYGIKIKDGGIEIKSIDPSTIP
                     GVPNLREVSLRKQIAEKDRERILVEADAERQRVLTLANAEEQRIKTVYGAVREMGEIG
                     KLLRTLEMLEKSTGAGSRWVILPGGITEMISQILPGRTIDGPPAPDGGVWIKGEELSS
                     LRNEISYLESLAGQVERRGLK"
     gene            1646..2137
                     /locus_tag="P5570_02130"
     CDS             1646..2137
                     /locus_tag="P5570_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39531.1"
                     /translation="MANEDLQKKYESLPGKLKRALFDPEIANRVYKIGESFNIPKEKM
                     ETLAQITGKVFLKIIDLDYLKDALKDHLEISLVDAEKLCQKLNKEVFSPYKKYLKETK
                     EETIGQIQQMNDQKQEKIKTKKELSSKEENDKYREPINDEEDIINPQVIVKDNDGRIK
                     KIL"
     gene            2221..2631
                     /locus_tag="P5570_02135"
     CDS             2221..2631
                     /locus_tag="P5570_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HRR39532.1"
                     /translation="MPQYYVPLFIESETQVFGPLSLFQFIVVGIAGVIVIIVLTATQN
                     LLLTFMAMIVAGMPAVFFAFGKINGEKAAKIFPLVLKFFLGEKVLLWQKRGEEGMNLK
                     EIQRVIEEKEKIERAVTKESKLKKLNWELQLGKK"
     gene            2649..3230
                     /locus_tag="P5570_02140"
     CDS             2649..3230
                     /locus_tag="P5570_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39533.1"
                     /translation="MSSTQDFLPIKEIKDDIVILKNGQWRGIIASSSLNFGLLSRDEQ
                     EAVIYQYQNFFNSLDFNIQILIESRKLNINAYLKELKEIEERQESELLRMQAEEYREF
                     IKSLSEMVNLMSKNFYVIVPFYPMFATRSKEENFNRNKTQFSDRLDYVISGLRRVGVH
                     SVQLGNEEIIELLWSFYNPADAEKGEIPVFPEI"
BASE COUNT         1062 a          641 c          555 g         1071 t
ORIGIN      
        1 tcttttctga tactggctgc gtttgtcctc cagtttgctc ttcagtttgc tcttcgtcct
       61 ctacttgctg gcttgtctct tcttctctag ctggttcttg ctgttctcct tgctgctgtt
      121 ctccttgctg ctgttctcct tgctgctctg gcgtttcttt taactggctt tcagtttgcc
      181 cattcttatc cccagtttta ttttcaacct tctcaaccac tttctcaaga agatcaactt
      241 cttgtctgag agcctcttca acgctctctt caagaacgat ctttgtttta aaggccgcct
      301 ctagtcggat tcgtgccctt cttagtcgtt caatttcgtc ttgaatcatt tttccacctc
      361 aaaattttgg ttgaaataat catttcaacc ctcttctctc aacctgccct gcaagagatt
      421 ctaaatatga aatctcgttg cgaagagaac ttagttcttc gcccttgatc catactcctc
      481 cgtccggagc cggaggacca tcaatggtcc ttcccggaag aatttgagaa atcatctcag
      541 taattcctcc tgggaggata acccaacggc ttcctgctcc agtggacttc tccaacatct
      601 ctaatgttcg aagaagtttt ccgatctccc ccatttctct gacggctccg tagaccgtct
      661 ttatccgttg ctcttcagca ttagcgaggg ttagaaccct ttgtctttcc gcatcggcct
      721 caaccaaaat gcgctcccgg tctttctctg caatttgttt cctaagcgaa acctctcgca
      781 gattagggac cccgggaatc gtggaagggt cgatgctttt gatctcaatc cccccgtctt
      841 tgatctttat cccataagag ctttcaaact cttttataag acctgttcct tttaactttc
      901 catatatttc tcctcctttt tcctgttttt tctttacaat ttcttcaaat tcaaaagcag
      961 agacatattc ccttataagg ggttttatcc tgttcatcac catctcactc cagtcctgca
     1021 catgaaataa tgccttataa gggtttatca ccctcattgt gacaagaatt tctatgtcaa
     1081 gaggaacgag gtttttgtct tctgctttat cgatcgatgc ccaataaaca tcatctacaa
     1141 tcaggacata gtccaaaatt tcatgacgat gcgaaacaac ctccccattg acatcaatgc
     1201 ctgtccagga aaacttatat ttataaattt cccaaaacgg tggaatgcca atccagtaaa
     1261 gtcctccaag agcgcgtttt ctcttgttgg ggcaagaaac tatgttccaa ttcttatcaa
     1321 atccacatcc ttctctcggc ataagaaagt ctacaaactt tcctgccttc attctcgctt
     1381 tgcactgtga ctcattcaca atggtcacaa aaaggtttct tggggccaga acataataaa
     1441 tattcaaaaa aagcagaata acaaccccaa ttccaagtag taatacttca atccacatct
     1501 tctcacctcc tttttgtcaa agaacttttt gaccagaatt tgttctggtc gtttttatta
     1561 tgtcctgaaa aataatattt gtcaaatttt ctgataaact ttactttgct ttttgttttt
     1621 tgtataattt aaaaaataaa agcatatggc caatgaggat ttacaaaaaa aatatgaatc
     1681 cttgcctggg aaattaaaaa gagcgctttt tgatccagaa attgccaata gggtttacaa
     1741 aattggggag tcctttaata ttccaaaaga aaaaatggaa acccttgctc agattacagg
     1801 aaaagtattt ttaaaaatta tagacctgga ttatcttaaa gatgccttaa aagatcattt
     1861 agaaatttct ttagtagatg ctgaaaagct ttgccaaaaa ttaaataaag aagttttttc
     1921 accttataaa aaatatctta aagaaacaaa agaagaaaca attgggcaaa tacagcaaat
     1981 gaatgatcaa aaacaagaaa aaataaaaac aaaaaaagag ctttcaagca aagaagaaaa
     2041 tgataaatat cgcgaaccga taaatgatga agaagatata ataaatcccc aagtaattgt
     2101 taaagacaat gacggtagga ttaaaaaaat actgtaaagt ttgaaaaacc taagcgctgt
     2161 taaaattaaa aggttaaggt tttgaaattt taattttgag tttagaattt acgtttagct
     2221 atgcctcaat actatgttcc attatttatt gaatcagaga ctcaggtgtt tggaccacta
     2281 tcgcttttcc aatttattgt tgtaggaatt gctggagtaa ttgtaattat tgttttgacg
     2341 gctactcaaa acctgctttt aacctttatg gcaatgattg tggcaggaat gcccgctgtt
     2401 ttttttgctt ttggtaaaat caatggcgag aaagcagcaa aaatctttcc cttggtttta
     2461 aaattttttt tgggagaaaa ggttttgctc tggcaaaaaa gaggagaaga aggaatgaat
     2521 ttaaaagaaa tacaaagagt aattgaagaa aaagaaaaaa tagaaagagc tgtaacaaaa
     2581 gaaagtaaac taaaaaaact taactgggag ttgcaactcg gtaaaaaata aggtaaattt
     2641 aataatatat gtccagcact caagattttt taccaattaa agagataaaa gatgacatcg
     2701 tgattttaaa aaatggccaa tggagaggaa ttatcgcctc ttcttccctt aactttgggc
     2761 ttttatcacg agatgaacaa gaagctgtta tttatcagta tcagaatttt tttaattcgc
     2821 ttgattttaa cattcaaatt ttaatagaat cgcgaaagct caatatcaat gcttatttaa
     2881 aggagctaaa agagatcgaa gaaagacaag aatcagaatt attgcgaatg caggcagagg
     2941 aatatcggga gtttatcaaa agtttgtcag aaatggtaaa tttgatgtca aaaaattttt
     3001 atgttattgt tcctttttat ccaatgtttg ctaccagatc aaaagaagaa aatttcaaca
     3061 gaaataaaac ccaattttca gatcggcttg attatgttat cagtggcctt cgccgagtag
     3121 gagtacattc ggtacaactg ggaaatgagg aaattattga attattatgg agtttttata
     3181 acccggcaga tgcagaaaaa ggagaaatac ccgtatttcc agaaatatag tatttaatgt
     3241 tgttgttgct tattaacaat caaaaatcag tagggagaaa tcagaaaaca gaaattatta
     3301 ttacataaat ttccaagctc caataacca
//
LOCUS       DAOTQD010000042         3279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_23033_length_3279_cov_7.673077, whole genome shotgun sequence.
ACCESSION   DAOTQD010000042 DAOTQD010000000
VERSION     DAOTQD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23033_length_3279_cov_7.673077"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1680
                     /gene="murF"
                     /locus_tag="P5570_02145"
     CDS             64..1680
                     /gene="murF"
                     /locus_tag="P5570_02145"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRR39534.1"
                     /translation="MIFNLYLSFFIFFWFLHILRRTFFWLYLWQLKEYRWDRFKDELQ
                     QNPRVLVPVSAIAALALLLFSPLLFKVTIFDKNLFEMLVFVFCLIFGLYSLYLLFNGK
                     WSLPRFTKKMIALSVFTICLAVVLFMIINPVWYLMLEGINPFLAVFVLTFEILLPFFV
                     CICVGVVQLPTFFAKRKIIKKAKEKREQFKDLIVIGVTGSYGKTSTKEMLFQMLSLKY
                     KVLKTEGNNNTEMGVAQTVLQKLKKNHQVFVCEMAAYKKGEIKAICDIVHPKIGILTG
                     ISHQHISLFGSFKNIIQTKYELINSLPEDGLAIFNGANKECQKLAQRTTIRKIVYSPE
                     ALVEEVEETADFIEFKLKDYPNIGKIRLNLLGRQNLENFLGALICALELKVPLKKIQE
                     LAPKLKPSVTAMRKTKGKNGVTIIDNSYSQNPDGVIAAIEYLKNFKGKKVVIMPCLIE
                     LGKSAPSVHKNIGRKIGEVVDLAIITTSYYFEELKLGAQEAGMKQSQIIFSKKPKKIL
                     EKLNPYFKKENVILIEGRINENLKSQIFKY"
     gene            1852..3126
                     /locus_tag="P5570_02150"
     CDS             1852..3126
                     /locus_tag="P5570_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRR39535.1"
                     /translation="MFSPISISFSPNTESDDVSLVLKLLFQPLKWQKGKAIKNLEETF
                     KNYLGVNYAFSFNSGRSALLGILVSMGVQEEDEVLLQAFTCNAVVNPILTLKAKPVFV
                     DIDDSLNLDPKDFKKKITKNSRAVIIQHTFGNSARIGEIKNICQKNNLFLIEDCAHSL
                     GAEYEGKKVGSFGDAAFFSFGRDKVISSVYGGMAVTNNNQIGQKLEIFQKTLDFPSRN
                     WILKQLLHPISFAIALPLYSLKIGKAILFLKQNLKILSPSVYPEEKRGELPKYFPKKM
                     PNAMAIMAQKQFQKLERFNKHRKEIAEIYNREFARINMPIYADNRKFEKVRICVNNGN
                     TERIYMRYSIFTERANEIREKLKEKNIFLNDGWHSQAIVPPGTDLEKMQYQKGSCPKA
                     EKVSKEILNLPTSIKITKKDAEKIIKEIKLII"
BASE COUNT         1202 a          440 c          549 g         1088 t
ORIGIN      
        1 ctcttttttt tattgctgtt ttttgatagt atattatcaa tatattatca aataataatt
       61 tttatgattt ttaatctcta tttatcattt tttatctttt tttggttttt gcatattttg
      121 cgccggactt ttttttggct ttatttgtgg caattaaaag aatatcgctg ggacaggttt
      181 aaagatgagt tgcaacaaaa tcccagggtg ctggtgccag tgagcgctat tgcggccctt
      241 gctttgcttt tgttttctcc gctgcttttt aaagttacta tatttgataa gaatcttttt
      301 gagatgctgg tgtttgtttt ttgtttgatt tttggtcttt attctttata tttacttttt
      361 aatggcaaat ggtctttgcc cagatttact aaaaagatga ttgctctttc tgtctttaca
      421 atatgtttgg cagtggtttt atttatgatt attaatcctg tttggtattt aatgttagag
      481 ggtataaatc catttttagc tgtttttgtt ttgacgtttg aaattttatt gccgtttttt
      541 gtctgcatat gtgttggagt tgttcaatta ccaacatttt ttgccaaaag gaaaattatt
      601 aaaaaagcta aagaaaaaag agaacaattc aaagatttaa ttgttattgg cgtaaccgga
      661 tcttacggaa aaacctcaac caaagaaatg ctttttcaaa tgttgtcttt aaaatataaa
      721 gtattaaaaa ctgaaggaaa taataatact gaaatgggag ttgctcaaac agttcttcaa
      781 aaacttaaaa aaaatcatca ggtttttgtt tgtgagatgg cagcttacaa aaaaggagaa
      841 ataaaagcaa tttgcgatat tgttcatcca aaaattggga ttttaactgg tatctctcat
      901 caacatattt ctctttttgg gagttttaaa aatattattc aaacaaaata cgagttgatc
      961 aattctctgc cagaagacgg ccttgctatt tttaatggtg cgaataaaga atgtcaaaaa
     1021 ttagctcaaa gaacaacaat aagaaagatt gtgtattctc ctgaagcatt ggttgaagaa
     1081 gttgaagaaa ctgctgattt tatagagttt aaattaaaag attatccgaa tattgggaaa
     1141 attcgattaa atcttttggg aaggcaaaat cttgaaaatt ttcttggggc tttgatttgc
     1201 gcactggaat taaaagtacc cttaaagaaa attcaagagt tagcccctaa attaaaacca
     1261 tcagtaacag caatgagaaa aacaaaagga aaaaatggag taactatcat cgacaatagt
     1321 tacagtcaaa accctgatgg tgtgattgca gcaatagagt atcttaaaaa ttttaaagga
     1381 aagaaggtag ttataatgcc ctgtttaatt gaacttggaa aatcagcgcc ttctgttcat
     1441 aaaaatattg gcagaaaaat tggagaagtg gtggatctgg caattatcac aacatcttat
     1501 tattttgaag aattaaaatt gggagctcag gaagcgggaa tgaaacaaag tcagattata
     1561 ttttccaaaa agcccaaaaa aattttagaa aaactaaatc cttattttaa aaaagaaaat
     1621 gttattttga ttgaaggaag aataaatgaa aatctcaaat ctcaaatttt caaatactaa
     1681 acactaaaca gaaatcagtt gtcagaaatc agaaaacagt agggaaagac ggaagacgga
     1741 aaacagaaat tgaaaattag aaattttaat aatttaatga aaatttaaaa tttaaaattg
     1801 aaaattatag ttttattttg atttttgatt tttgattttt gaattcaaat tatgttttct
     1861 cctatttcta tttctttttc tcctaatact gaatctgatg atgtttcttt ggttttaaaa
     1921 ttacttttcc aacccttaaa gtggcaaaag ggtaaagcaa ttaaaaattt agaagaaact
     1981 tttaaaaact acttaggggt aaattatgct ttttctttta acagtggtcg ctcggccctc
     2041 ctgggaattt tagtgtcaat gggcgttcag gaagaagacg aggttttgtt gcaggcattt
     2101 acctgcaatg ctgtagtaaa tcctattttg actttaaaag caaaaccagt ttttgtggac
     2161 attgatgata gtttgaattt agatccaaaa gattttaaaa aaaagattac taaaaactca
     2221 agagcagtaa taattcaaca tacttttggc aactcagcaa gaattggcga gattaaaaat
     2281 atttgccaaa aaaataattt atttttaatt gaagattgcg ctcattcttt aggagcagag
     2341 tatgaaggca aaaaagtggg aagtttcggc gatgcagcat tttttagctt tggcagggac
     2401 aaggtaatct cttcagttta tggaggaatg gcagtgacca acaataacca aataggacaa
     2461 aaattggaaa tttttcaaaa gacccttgat tttccttcaa gaaattggat tttaaagcaa
     2521 cttttacacc cgatttcttt tgctattgcc ttgccacttt attcgttaaa aattggaaaa
     2581 gcaattttat ttttaaaaca aaatttaaaa attttatcac catcagttta tcccgaagag
     2641 aaaagaggcg aattgccaaa atattttccc aaaaaaatgc cgaatgcaat ggcaataatg
     2701 gctcaaaaac aatttcaaaa attagaacga tttaataaac accgaaaaga aatcgctgaa
     2761 atttataatc gcgaatttgc gcgaataaat atgccaattt acgctgacaa tcgcaaattt
     2821 gaaaaggtgc gaatttgtgt aaataatggc aatactgaga gaatttatat gcgttattcg
     2881 atatttactg aaagggcaaa cgaaattcga gaaaaattaa aagaaaaaaa tattttttta
     2941 aatgacggat ggcatagcca ggcaattgtt cctcccggca ctgatttaga aaaaatgcaa
     3001 tatcaaaaag gcagttgccc taaagcagaa aaagtttcaa aagaaatttt gaatttacca
     3061 actagcatta aaattacaaa aaaagacgct gaaaaaatta ttaaagaaat aaaattgata
     3121 atttagggta gattcaagtt tttaacgcaa caaccccttt ttgtctttga cacgatcgta
     3181 aaaattcaaa gcaccaaata ccaaatacca aacaaatcca aaatactaaa cagaaatcag
     3241 ttgtcagaaa acagaaaaca gaaattatta ttacataaa
//
LOCUS       DAOTQD010000043         3237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_23391_length_3237_cov_10.080453, whole genome shotgun
            sequence.
ACCESSION   DAOTQD010000043 DAOTQD010000000
VERSION     DAOTQD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23391_length_3237_cov_10.080453"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..690
                     /locus_tag="P5570_02155"
     CDS             85..690
                     /locus_tag="P5570_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRR39536.1"
                     /translation="MEKLLIATSNPGKLKEYKELLKGLPLEFVSLKDLEIDAKATEDG
                     KNFNENAIKKAKFYSKLTGLPTLSDDGGLEIDYLNGDPGVNSHRWPGHEATDEELVKF
                     TLKKMKGVPWKKRGAQLKVVIALKIPGKKVFTSEGKIRGFILTKPREKIIPGYPFRSI
                     FYLPQYRKSFNQLSEEEELKISHRKKAIKEIIKFLKSEKII"
     gene            694..1206
                     /gene="amrA"
                     /locus_tag="P5570_02160"
     CDS             694..1206
                     /gene="amrA"
                     /locus_tag="P5570_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRR39537.1"
                     /translation="MHPLVDLAKKAVESYVKNGKVIEPPAGLPEECLKKKAGTFVTIE
                     KNNDLRGCIGTYLPTKENIAQEVISNAIAAATRDDRFGPIQKEELPLLSYTVYVLNKP
                     EIVKDLKELNPKKYGVIVQSGFKSGLLLPDLEGVNTIKEQLFIACQKAGIDPTVEPVT
                     VYKFSTKKYS"
     gene            1237..3225
                     /gene="priA"
                     /locus_tag="P5570_02165"
     CDS             1237..3225
                     /gene="priA"
                     /locus_tag="P5570_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRR39538.1"
                     /translation="MYITDVVPLTKIPQPLPQILSYFTSQKLEKGSLVLIPLQKKNTE
                     AIVFSQKEAKDLKQEIKKADFALRPILEVIEKDPLLDERQLNLAKWISDYYFASFGKV
                     LKLFLTANKKSQSQLKKPIQSSTKSKLNKSIKPILYFSPPDFLPENEIKETLFEKKEI
                     LLLVPEKNKIDFFRKKIAKIQNQNNKNLQITIAARSGLFFPFQKLGLVIVSDVDNESY
                     KEQREPRLFAPDVAQKLAEIWGAKLIFLSVLPGADYYFKAKTGKILWKKIENKKEIQK
                     VEININSLKNPGDKNDSLTSLKDYNSLSAKRSYFLKNSNKPKNDQKNWSPFSSVVLKE
                     IEKYLELKKKILLFINRKGTATNLLCQDCGWQKFCKNCGVPLTYHINHQPSTINHPMM
                     VCHYCGFKNNVPEYCENCKSWRLTTLGIGAEEVEKEIKNKFPQAKIFRLDREISPKIK
                     DQKKTLQEFFEKGDILVSTSLIFSYIFARYVDKSLRAEKELKFALTVFVSFGAQLSLP
                     DFRFEEKLRRQIEKLCFLTSEKFLLQNRSKREILGKSLKDFYNKELEERKKYFYPPFS
                     QLIKLTFKHKNLEITKKEAYVLKEKLEKQLNNFLTNNSQSTVRVLGPVPAFIFKIKGR
                     YRWNMLIKIKRSETRIQNKLLQVVPPEWEVDVDPINFF"
BASE COUNT         1219 a          477 c          559 g          982 t
ORIGIN      
        1 tgtgggttcg attcccacca ggtccaaaaa tttaaataga ggggagcaat cttttgaatt
       61 ataaataatg gagtttaaga atttatggaa aaattattaa ttgcaacaag taatccaggc
      121 aaattgaagg aatataagga attattaaaa ggtttgccgt tggaatttgt ttctttaaaa
      181 gatttagaga tagatgctaa agccactgag gatggtaaaa actttaatga aaatgctatt
      241 aaaaaggcaa aattttattc taaattaact ggcctaccga ccttgtctga tgatggtggt
      301 cttgagattg attatttaaa tggtgatcct ggcgtaaact ctcaccgctg gccaggacat
      361 gaagccacag atgaggaatt agtaaaattt accttaaaaa aaatgaaagg agttccttgg
      421 aaaaagcggg gagcacaact taaggtagta attgcgctta aaataccagg gaaaaaggtt
      481 tttacatctg agggaaaaat aagaggattt attttaacaa agcccagaga aaaaattatt
      541 cctgggtatc catttcgttc aattttctat ttgccacaat atagaaaatc ttttaatcaa
      601 ttaagtgaag aggaggaatt aaaaataagt catcgcaaaa aagcaataaa agaaataatc
      661 aaatttttaa aatcagaaaa aattatttga aatatgcacc cattagttga tcttgctaaa
      721 aaagcagtag aaagctatgt taaaaatgga aaggtgattg agccaccggc tggtttgcca
      781 gaggagtgtt taaagaaaaa agctggtact tttgtgacga ttgaaaaaaa taatgacctg
      841 cggggctgca ttggtaccta tttgccgacc aaagaaaaca ttgctcaaga agtaatttca
      901 aatgctattg ccgctgctac cagagatgat cgttttggcc cgatccaaaa agaagaatta
      961 ccattacttt cttatactgt atatgtttta aacaagccag agatagtaaa ggacctaaaa
     1021 gaattaaacc ctaaaaaata cggcgttatt gttcaaagcg gatttaaatc aggtcttctg
     1081 ctgcctgatt tggaaggagt aaacactatt aaagagcaac tttttattgc ctgccaaaaa
     1141 gcaggaatag atcccactgt tgaacctgtt acggtatata aattcagcac aaaaaaatac
     1201 tcctaatttt ttgcttttac tttttgcatt tttattatgt atattaccga tgttgttccc
     1261 ttaacaaaaa ttccccagcc cctgcctcag atattatctt attttacttc tcagaaacta
     1321 gaaaaaggaa gtttggtttt gattcctctc caaaaaaaga acaccgaggc aattgtgttt
     1381 tctcaaaaag aagcaaagga cttaaagcag gaaattaaaa aagcagattt tgcgctcaga
     1441 cctattttag aagtgattga aaaggatcct cttttagatg agagacaatt aaatcttgca
     1501 aagtggattt ctgattatta ttttgcttct tttggcaagg ttcttaaact cttcctaaca
     1561 gcaaataaaa aatctcaaag tcagttaaaa aaaccaattc aatcaagcac aaaaagcaaa
     1621 ttaaataaat caattaagcc cattctttat ttttctcctc ctgatttttt gccggaaaat
     1681 gaaataaaag aaactctttt tgaaaaaaag gaaattttat tactggttcc agaaaaaaat
     1741 aaaattgatt tttttaggaa aaaaatagca aaaattcaaa atcaaaataa caaaaatttg
     1801 caaataacaa ttgctgcccg ttcgggatta ttttttcctt ttcaaaaact gggactggtt
     1861 attgtttctg atgttgataa cgagtcttat aaagagcaaa gagaacccag gctttttgca
     1921 cctgatgttg ctcagaaatt ggctgagatt tggggagcaa aattgatttt tttatctgtt
     1981 cttcctggtg ctgattatta ttttaaagca aaaacaggaa agattttatg gaaaaaaata
     2041 gagaataaaa aagaaataca aaaagttgaa ataaatataa attctcttaa aaatccagga
     2101 gacaagaatg attctttgac ttcacttaag gactataatt ctttaagcgc taagcgttct
     2161 tattttttga aaaattcaaa caagccaaag aatgatcaaa aaaattggag ccctttttct
     2221 tcagttgttt taaaagaaat tgaaaaatac ctggaattaa aaaagaaaat tttattattt
     2281 ataaatcgga agggtacagc aacaaatctt ttgtgtcagg attgtggctg gcaaaaattt
     2341 tgtaaaaact gtggcgtgcc attaacctac cacatcaacc atcaaccatc aaccatcaac
     2401 catccaatga tggtatgtca ttattgcggt tttaaaaaca atgttccaga atattgtgaa
     2461 aattgcaaaa gctggcgact tactactctt ggaataggag cagaggaagt ggaaaaagaa
     2521 ataaaaaata aattccctca agcaaaaata ttccgattag atagggaaat atctccaaaa
     2581 ataaaagacc aaaaaaagac tcttcaggaa ttttttgaaa aaggcgatat tcttgtttcc
     2641 acctcgctta ttttttctta tatttttgct cgctatgtcg ataaatcttt aagggcagaa
     2701 aaagaattga aatttgcttt aacagttttt gtttcttttg gcgcgcaact ctcattgcct
     2761 gattttcgat ttgaagaaaa attgcgtcgg cagattgaaa aattgtgttt tttgaccagc
     2821 gaaaaatttt tattgcaaaa tcgttctaaa agagagatct tgggtaagtc cttaaaagat
     2881 ttttataaca aagagctaga agagaggaaa aaatattttt atccgccatt ttctcagttg
     2941 ataaagttaa cttttaaaca caaaaatcta gaaataacaa aaaaagaagc atatgttttg
     3001 aaagagaagc tagagaaaca attaaataat tttttaacta acaactcgca atcaacagtt
     3061 cgggtccttg gtcctgtgcc ggcttttatt ttcaaaatca aaggaagata cagatggaat
     3121 atgcttatta aaattaaaag atcagaaact agaatccaga ataaattatt acaggtagta
     3181 ccacctgagt gggaagtaga tgtagatcca atcaattttt tttaaaggag taattaa
//
LOCUS       DAOTQD010000044         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_23602_length_3215_cov_11.005380, whole genome shotgun
            sequence.
ACCESSION   DAOTQD010000044 DAOTQD010000000
VERSION     DAOTQD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23602_length_3215_cov_11.005380"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5570_02170"
     CDS             complement(<1..204)
                     /locus_tag="P5570_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HRR39539.1"
                     /translation="MAKDGTARGGARIGSGKKPTNKKKVEVLTTTFVDMSDFITPDEI
                     EGVEIPPIKDYLKAKQKNGKDLYA"
     gene            complement(208..645)
                     /locus_tag="P5570_02175"
     CDS             complement(208..645)
                     /locus_tag="P5570_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRR39540.1"
                     /translation="MTYYYIAGFTKSGSALDSALTEKIFQTKAEGLKELKSYQFKDGD
                     IAHKLVLYGYDEETETGIAIASLEKAETSFGKLLKEKRQKAEMTQQEVADKIGVPLVT
                     YQRWELDIHYPESYNLLALINLFKLEDYDMQKTRHKFTKLRGD"
     gene            complement(787..1767)
                     /locus_tag="P5570_02180"
     CDS             complement(787..1767)
                     /locus_tag="P5570_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39541.1"
                     /translation="MNRKSVGDERKEVRQLMPLIFVILILLADFAAVFFLSALSADFD
                     LSVLKTSEFWANYVITLLLIFIAYFALIFYGNHKGNKSDEIISAQSDLYRAQKEVTIN
                     LLSGELEKHSNSKNVISKCYAIINRLDKKLRRIKKEGKRLKIIDEKKATVYTIHLEEE
                     KIAERTLTDAERQERLDFIEKLNYNPNNYLFIKYRKIDKRIIFSSKRGSSNKIGKFSA
                     RSIIFSESLIKISFSMICTLAFTLLLYGSYKWDSNAWFNLIWRFLLVCFNAYMGYELG
                     YKMIVGDKRAALLERLDVFNEFKAFCRKAGYLKPKDQEQEKKQEEETKGA"
     gene            complement(1760..2263)
                     /locus_tag="P5570_02185"
     CDS             complement(1760..2263)
                     /locus_tag="P5570_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39542.1"
                     /translation="MITRKEYFKKRWLYFIAIAFIFIVPFIILLEGVFNNNADKRYPL
                     QLSVVIIGVVYFTALLKFFYKKIMLMKPSVGKIFLERSMLIVPLLTLACLVFLVEKIF
                     VNFDFYLWMIVGSMTIGTFIEALEYLINRKFIYKWEIFLKAKEEVDRKRAEEEYRKVL
                     EEIEHNE"
     gene            complement(2273..2779)
                     /locus_tag="P5570_02190"
     CDS             complement(2273..2779)
                     /locus_tag="P5570_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39543.1"
                     /translation="MAEIINQIIAFFTSDEFQALMTSIGSLLLAVSPFLFKLITSKIE
                     KWKIKFQAKASEVAEALKLVDEYKKLAEAEAAKTKQTIDQLTELVNKQNEAMRLAFDR
                     SNLKEDTKQAIHKILQVQTVKIPVEPLLEAAKTVLKEDEKKKDDSLSTQTPVEELEEE
                     PQYTRIFK"
     gene            complement(2794..3114)
                     /locus_tag="P5570_02195"
     CDS             complement(2794..3114)
                     /locus_tag="P5570_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39544.1"
                     /translation="MLNPKILKENKLIIEGYQAKENARQHGVLICTDAKNKTFEFVKV
                     RDLPYQIEDDGTIMTIGEYLDTLENIVAANYEDIIQTLNQKVEMLNQAILALADQIDK
                     DKFL"
BASE COUNT          939 a          601 c          491 g         1184 t
ORIGIN      
        1 agcatataag tcttttccat ttttttgctt tgcttttaaa taatctttaa tcggtggaat
       61 ttcgactcct tcaatttcat cgggtgtaat aaaatccgac atgtcaacga aagttgtagt
      121 taacacctcc acctttttct tgtttgtagg tttctttcca ctacctattc ttgcaccacc
      181 tcgagcagta ccgtcctttg ccatcggtta atccccccgc agttttgtaa atttatgtct
      241 tgttttttgc atatcataat cttctaactt aaacagatta atgagtgcta gaagattata
      301 agattccgga tagtgaatat ctaattccca ccgttgatat gttactaaag gaacaccaat
      361 cttatcagcc acttcttgtt gtgtcatttc ggctttttgt ctcttttctt ttaatagctt
      421 gccgaaagat gtttcggctt tttccaggct tgcaatagcg atacctgttt cggtttcttc
      481 gtcatagccg taaagaacta atttgtgagc tatatccccg tctttaaatt gatagctttt
      541 taattccttc aggccttcgg cttttgtttg aaaaattttt tccgttaatg cgctatcaag
      601 agcggatccg gattttgtaa atccggcaat gtaataataa gtcattttct ttcctccttt
      661 acgctttaat tatactgcga ttgcgtagta atgtcaagac ttttgataaa aaaattaata
      721 ttttttttgc agaagtcgcc caaacaatca aagtgcttgt ctagaataaa taaaaaaaga
      781 gggccgctaa gcccccttag tttcttcttc ttgttttttc tcttgttctt ggtcttttgg
      841 tttaagatag cctgctttcc gacaaaaggc cttaaactcg ttaaatacat ccaaacgttc
      901 tagcagagct gctctcttat cgccaacaat cattttataa cccaattcat agcccatata
      961 ggcattgaag cagaccaaga gaaaacgcca tattaaatta aaccaagcgt ttgaatccca
     1021 tttatatgaa ccatacaaaa gtagagtaaa cgccaaagtg caaatcatag agaaagatat
     1081 tttgattaac gattcgctaa agatgattga tctagcgcta aatttaccga ttttattact
     1141 cgaacctctt tttgaagaaa aaatgattcg tttatcgatt tttcggtatt taatgaacaa
     1201 ataattattt ggattgtagt ttaatttttc tataaagtct aatctttcct ggcgctctgc
     1261 atccgtcaat gttctttcgg ctatcttttc ttcttcaaga tgtattgtat aaaccgtagc
     1321 ttttttctca tcgatgattt ttaatcgctt tccttctttc ttaattcggc gaagtttttt
     1381 gtctaaacgg tttatgattg cataacactt ggaaataaca tttttcgaat tagagtgttt
     1441 ttctagttcg ccagacaata gattgatcgt tacttctttt tgcgctctgt aaaggtcgct
     1501 ttgggccgag ataatctcat cgcttttgtt gcccttatga ttaccataga aaattaaagc
     1561 aaaataagca ataaaaatta gcagcaatgt tattacataa ttagcccaaa actcgcttgt
     1621 tttcaatacc gacaaatcaa aatcagctga taaagctgag agaaaaaaga cagctgcaaa
     1681 atcggctaat agaattagta tgacaaagat taaaggcatc agttgcctaa cttcttttct
     1741 ttcatcacca acagattttc tattcattat gctctatttc ctccaagact ttacgatatt
     1801 cttcttcggc tcttttacga tccacttctt ctttagcctt taaaaagatc tcccatttat
     1861 agataaactt gcgatttatt aaatattcta acgcttcaat aaatgttccg atagtcattg
     1921 agccaacgat catccacaaa taaaaatcga aattaacaaa tatcttttct actaaaaaaa
     1981 ccaaacaagc tagtgtgagt agtggaacga ttagcattga acgctctaaa aagattttac
     2041 caacgcttgg cttcataagc ataattttct tataaaaaaa ttttagtaat gcagtaaaat
     2101 acactactcc gattattaca actgaaagct gcaacggata acgtttatcg gcattattat
     2161 taaaaacgcc ttccaaaaga attataaaag gcacaatgaa gataaaagcg atagctataa
     2221 aatataacca tcgcttttta aaatattctt ttctggtaat catatttacc acctatttga
     2281 agatacgagt gtattgtggt tcttcttcta gctcttctac gggagtttga gtggataaag
     2341 aatcatcttt ctttttttca tcctctttaa gaacggtttt ggctgcctct aaaagcggct
     2401 cgacaggaat tttgactgtc tgtacttgta gaattttgtg gatggcttgc ttagtatctt
     2461 cctttaagtt gctacggtca aaagccaatc tcattgcttc attttgcttg tttacaagtt
     2521 cggttagttg atcgatggtt tgttttgtct ttgctgcttc tgcttctgca agtttcttat
     2581 actcatcaac aagttttaag gcttctgcaa cctcactcgc tttagcttga aacttaattt
     2641 tccatttttc gattttagaa gtaataagct taaatagaaa tggcgataca gcaagcaaca
     2701 atgacccgat agaagtcatt aaggcttgaa actcatcgct cgtaaaaaat gcaataattt
     2761 gattaataat ttctgccatt tattgtttcc tccttatagg aatttatctt tgtcgatttg
     2821 atcagctaac gctaaaatcg cttggtttaa catttcgact ttttgattta gagtttgaat
     2881 tatatcttca taatttgcgg cgacaatatt ttcgagtgta tcgagatatt cgccaatcgt
     2941 cataatagtt ccatcgtctt caatttgata gggcaaatct cgcaccttaa caaattcaaa
     3001 tgttttgttt ttagcatcag tgcaaattaa aactccatgt tgtctggcat tttctttcgc
     3061 ttgatagccc tcgataataa gtttattttc ttttaagatt ttaggattga gcatattttt
     3121 ttaatactcc tttattctgt attgttttta attctttatt tttaggaaac aacttcccct
     3181 ttaaataaat tgttagtgga atagttacaa ccttt
//
LOCUS       DAOTQD010000045         3203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_23714_length_3203_cov_10.306544, whole genome shotgun
            sequence.
ACCESSION   DAOTQD010000045 DAOTQD010000000
VERSION     DAOTQD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23714_length_3203_cov_10.306544"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1327)
                     /locus_tag="P5570_02200"
     CDS             complement(287..1327)
                     /locus_tag="P5570_02200"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRR39545.1"
                     /translation="MKTLREANINKGTRVLVRCDFNCPIDEKGNLLETYRIEKTIPTI
                     NYLLKKQARIILMSHLDDPKGKVVEKLRLTSVADALFSYLDLSILKAPDCVGKNIEQM
                     VKEMQEGEILLLENLRFHKEEEENNKLFSKKLALLGDLYVNDAFGVCHRAHSSIVGVP
                     DFLPSFAGFLLESEIKNLNKVLENPKRPLVAIFGGTKIETKLPIIQKFLDLADFVLVG
                     GKIAKEINREKDEYKNNLNLYNALDFNQGFDIGDKTIKEFKNIILKAKTIIWNGPMGY
                     FEKSEFEKGTKEIAKAIAENDGFKVAGGGETLMAIIKYGLEKHFDFLSTGGGAMLDFL
                     AGKKLPGIEAIK"
     gene            complement(1444..2463)
                     /locus_tag="P5570_02205"
     CDS             complement(1444..2463)
                     /locus_tag="P5570_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRR39546.1"
                     /translation="MFDVITFGSATQDVFLKSKGFIVREEKKVFTKKGICFSLGSKIP
                     VQEIKFLNGGGGMNSAFTFKSQKFNVAYCGVVGDDVPGEEIRRKLIQNKISTDFLFFD
                     SKKPTNYSVVLETANERTILVYKGASELLVEKKIPWSRLKSTKWFYLAPFSGKLVDLW
                     EDILIFAQKNHIKVVTNPGNSQLILPKDRLKKLLNLADVLLLNQEEASLATGLPYNKE
                     KEIFKKLDNWVEGICVMTKGPLGGVVSDGKYLYEYGILKAPKTLDRTGCGDAFGSGFV
                     TGLIRWSSALQKKWGLNQIEYAIQVGSANAVSSIQEFGAQNGLLKKGDDIFKFGKVKI
                     KKRKL"
     gene            complement(2473..2931)
                     /locus_tag="P5570_02210"
     CDS             complement(2473..2931)
                     /locus_tag="P5570_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39547.1"
                     /translation="MSKLNNILISVIVVLLIVLGIVIYWQNGGFEKNYWAVYLDSGDL
                     YFGKLSNFPQLSLTDVWFLQSNLNNEENSFGIEKLTNVFWGPKNQLYLNQENIIWKVE
                     LEDDSQVVQFIKSNQNQNKETTEEEGLEDLKDLENLEENESSDSSSESNK"
BASE COUNT         1029 a          623 c          395 g         1156 t
ORIGIN      
        1 tatttttttc ttcttctgtt aacagttttg atacttgagc agcattaatc gtttgatcat
       61 cttcattttt ttcaaatggg aaaataaaaa aaagcgaaat caaaacgatc accacaatta
      121 agataaaaat aaaaaatgat tttttcattg tttgtaatat aaaatgcaaa acaacaatag
      181 atatttgtag atatttattg ttacaatgaa tatcgcgagc gaagcgagca agtatctatc
      241 aatatcgtgc tgaaggcaca agtatctata aattttgctt tttgatttat tttattgctt
      301 ctatccctgg taattttttc ccagctaaaa aatcaagcat tgctcctccg cctgttgaaa
      361 gaaagtcaaa atgtttttct aggccatatt ttataatagc cataagcgtt tcacctccgc
      421 cagcaacttt aaaaccatca ttctcggcaa ttgcttttgc aatctctttt gtcccctttt
      481 caaattctga tttttcaaaa taacccattg gcccattcca aattattgtt ttcgctttta
      541 aaataatatt tttaaactct tttatggttt tatctccaat atcaaatcct tgattaaaat
      601 ctaaggcatt ataaagatta agattgtttt tatattcatc tttttctcga ttaatctctt
      661 ttgcaatttt cccccctacc aagacaaaat ctgctaaatc aagaaacttt tgaattatcg
      721 gcagttttgt ttcaattttt gttcctccaa aaattgctac caaaggcctt ttcgggtttt
      781 ctaaaacctt attcaaattc ttaatctcgc tctctaataa aaatccagcg aaacttggta
      841 aaaaatcagg aacacctaca attgaactat gggcacggtg acaaactcca aaagcatcat
      901 taacataaag atctcccaat aaagctaatt tcttggaaaa cagtttgttg ttttcctctt
      961 cttctttgtg aaatcttaaa ttttctaaaa gcaatatttc tccttcctgc atttctttta
     1021 ccatttgttc tatatttttt cccacacaat caggcgcttt taaaattgat aaatcaagat
     1081 atgaaaaaag agcatcagca acagatgtta atctcaattt ttcaaccacc ttgcctttag
     1141 gatcatctaa atgactcatt aaaataatcc gtgcttgttt ttttaaaaga taattaatag
     1201 taggtatagt tttttcaatc cgataggttt ctaaaagatt acccttttcg tcaataggac
     1261 aattaaaatc acaacgcact aaaacccgcg ttcctttgtt gatattggcc tcgcgtaaag
     1321 ttttcataaa ataataaatc aaaaatcaaa attgatagat acttgctcgc ttcgctcgcg
     1381 atattcattg taacaataaa tatctacaag tatctattgt tgttttgcat tttgcattaa
     1441 caattatagt tttctctttt taatctttac ttttccaaat ttaaaaatat catctccttt
     1501 ctttaaaaga ccattctggg cgccaaactc ttgaatagaa gatacggcat tggctgatcc
     1561 aacttgaata gcatattcaa tttgattcaa tccccatttt ttttgcaaag cagaagacca
     1621 ccttattagt ccagttacaa aaccagatcc aaaagcatct ccacagccag ttcggtctaa
     1681 agtttttggc gcttttaaaa tgccatattc ataaagatat tttccatctg aaactactcc
     1741 ccctaaagga ccctttgtca ttacgcatat cccctctacc caattatcaa gttttttaaa
     1801 gatctctttt tccttattgt atggcaaacc agtggccaaa gatgcctctt cttggtttaa
     1861 aagtaaaaca tcagctaaat ttaataattt ttttaagcga tctttgggca aaataagttg
     1921 gctattgccc ggattcgtta caaccttaat atgatttttt tgggcaaaaa ttaaaatatc
     1981 ttcccataaa tctaccaatt tgccagaaaa tggtgctaaa taaaaccact ttgttgattt
     2041 taaacgagac caaggaatct ttttttctac caacaattct gacgctcctt tatataccaa
     2101 aatcgttctt tcattagctg tttctaatac aacagagtag ttagtaggtt ttttactatc
     2161 aaaaaaaaga aagtcagttg aaattttatt ttgtatcaat tttcttctta tttcttctcc
     2221 tggcacatca tctcccacta caccacaata agccacatta aatttttggc ttttaaaggt
     2281 aaacgcacta ttcatgccac cgccgccatt taaaaactta atttcctgaa ctggaatctt
     2341 tgatcccaaa gaaaaacaaa ttcctttttt agtaaaaact tttttttctt ctcttactat
     2401 aaatcctttg ctttttaaaa aaacatcttg ggtagcagaa ccaaaagtaa taacatcaaa
     2461 catattattt aattatttat ttgattcgga acttgaatcg gaagattcat tttcttctag
     2521 attttctaga tcttttagat cttctagacc ttcttcttct gttgtttcct tattttgatt
     2581 ttgattggat tttataaatt gcactacctg actatcatcc tctaattcaa ctttccaaat
     2641 aatattttct tgattaagat acaattgatt ctttggtccc caaaaaacat tggttagctt
     2701 ttctatacca aaagaatttt cctcgttatt aagattgctt tgcaaaaacc acacatctgt
     2761 taatgacaat tgcggaaaat tactcaattt tccaaaatac aaatcaccac tatctaaata
     2821 aaccgcccag taattttttt caaatcctcc attttgccag taaataacaa ttcccagcac
     2881 tattaataaa acaacaataa cacttattaa gatattattt agtttagaca tattttaaaa
     2941 aattattatt tatttatatt atatcgacct ttcaacttgg gtaggttcaa atttttaaca
     3001 cagcaatgtc acgggatcgc cacgtcgctc acgcgagccc cgtaagaaat ctttgatttc
     3061 gcacggggcg aagcgttgaa aagaagataa aatcttcttt gaacgctctt cgcgatgaca
     3121 agaaaaagga gtttatcttc ttttcaaaac ttcgcttcgc tgcgctaccg tttaacgtga
     3181 tacggtaaac gttggcgttg cgc
//
LOCUS       DAOTQD010000046         3085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_24816_length_3085_cov_7.510891, whole genome shotgun sequence.
ACCESSION   DAOTQD010000046 DAOTQD010000000
VERSION     DAOTQD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24816_length_3085_cov_7.510891"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="P5570_02215"
     CDS             complement(<1..599)
                     /locus_tag="P5570_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRR39548.1"
                     /translation="MFILSIDTSCDDTCISIIKSKKQKAKSKNTNFEILSNVVSSQVK
                     VHAKYGGIVPYLAKREHQKNLAMVFKKTLAKSKIQNPKIIDFIAVTIGPGLEPCLWQG
                     INFAKNLANDLKKPIVPVNHLEGHILANFLKPINKSLKSKTLKIFPAVGLVVSGGHTE
                     LFLMKNIGKYKLLGETRDDAAGECLDKIARILNLEYPGGP"
     gene            760..1188
                     /locus_tag="P5570_02220"
     CDS             760..1188
                     /locus_tag="P5570_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39549.1"
                     /translation="MYEDQIERLKREAQSIERDISRINYQEDILTQKIRQYQIAQQRS
                     PQSAAAYARQIDDLERQIDKLRSDRINASSRLSQKEREISLWEKRSTEAKAKQRSQYP
                     QGGSSIRRYGIPPRGGNDNDSSRGSSGPMGPIGPGTGTGI"
     gene            1264..>3085
                     /locus_tag="P5570_02225"
     CDS             1264..>3085
                     /locus_tag="P5570_02225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRR39550.1"
                     /translation="MQSNELREKFLNFFKKRGHLIVPSSSLIPEDKSVLFTTAGMQQF
                     SLYLSGKKDPIKDFSTRHLTSCQKCFRTNDIEEVGDDTHHTFFEMLGNWSIGQDEDGY
                     FKEGAIGYALEFFADTLSLDKDKFYITIFKGENNIKRDEESLKIWQKNGIPKERIFEF
                     GKDNFWGPVSETGPCGPCSEIHYDRGEEFGCGRPDCGPNCKRCKRFVELWNLVFMEYW
                     KDEKGDYQKLPQTNVDTGIGFERLLSVLERKSSAYETDLLLPVIQEIEKLSPNAYKLN
                     PKACRIIADHLRGACFLISEGIIPGNVDRGYILRRLIRRTVRYSRILNLKDLWYKNPV
                     EKIITKYSVVYPELKSNEEKILTVWQNEEEKFSKALSRGLKEFEKLIERERRQGKNLI
                     SGREAFNLYESYGFPLELTEEMAKEKGIIVDKLGFKKSLKEHQKISRFGAEKKFGGHG
                     IIQNPKFKSQNSKVVQLHTATHLLQSALRQVLGPEVKQMGSDINEERLRFDFSFPRKL
                     TKEEIQRIENLVNQKIKENLKVEREEMKLEDALSSGALAFFREQYPEKVSVYKIFNPK
                     NNEVFSKEICAGPHIERTGVLGKFKIKKEESSGAGVRRIKA"
BASE COUNT         1056 a          437 c          603 g          989 t
ORIGIN      
        1 ggtcctcctg gatattcaag atttaaaatt cttgctattt tatccaaaca ttcaccagcc
       61 gcatcgtcgc gtgtttctcc taaaagttta tatttgccta tatttttcat caaaaaaagt
      121 tcagtgtgtc caccactaac taccaatccc accgctggaa agattttaag agttttggat
      181 ttaagacttt tgttaattgg ttttagaaaa ttagcgagaa tgtgcccttc caaatgatta
      241 actggcacaa tgggtttttt aaggtcattt gccaaatttt tggcaaaatt aattccttgc
      301 caaagacagg gttcaaggcc tgggccaatt gttactgcaa taaaatcgat tatctttgga
      361 ttttgaattt ttgattttgc caatgttttt ttaaacacca ttgccaggtt tttttgatgt
      421 tctctttttg caagatacgg cactattcca ccatactttg catgaacttt cacctgtgaa
      481 gaaactacat tagaaagaat ttcaaaatta gtatttttgc tttttgcttt ttgctttttg
      541 ctttttatta tgcttatgca agtatcatcg caagatgtat caatagaaag aataaacata
      601 gattagtatt ttaatatttt ttatgttaat tgcttttatg tttttatgat aaaataattt
      661 gaattataaa gcaacttttg ttaaaatctt aacagaaccc agtgaaattt attaaatttt
      721 tattctggga aaggtcgcat tatgttattt ataaaaatta tgtacgaaga tcaaatagaa
      781 agattaaaaa gagaagccca atctattgaa agggatattt caaggattaa ttatcaagag
      841 gatattttaa cgcaaaagat tcgtcagtat cagatagctc aacaacgatc acctcaatca
      901 gcggctgctt atgctcgtca aatagacgat ctggagcgtc agatagataa acttcgcagt
      961 gatagaatta atgcttcatc aagattaagt caaaaagaaa gggagatttc attatgggaa
     1021 aaaagatcaa ctgaggcaaa agcaaaacaa cgaagtcaat atccccaagg tggttcgtct
     1081 atccggagat atggcatacc tcctcgcgga ggcaatgaca atgattcttc tcgcggttca
     1141 agcggaccaa tgggacctat agggcctggt actggcacag ggatttaaga atatttactt
     1201 agttttgaag ctgcttttta aagtagcatt atttctaatg ataattattt tttgtattct
     1261 tttatgcaat caaatgaatt gcgggaaaaa tttttaaatt tttttaaaaa aagaggacat
     1321 ttaattgtac cttcaagttc acttattccc gaagataaat cagttctttt taccactgct
     1381 ggaatgcagc agttttcttt atatttgagc ggaaaaaaag atccgattaa agattttagt
     1441 acccgccatt taacctcctg ccagaaatgt tttcgaacaa atgatattga ggaagtaggt
     1501 gatgataccc atcatacatt ttttgaaatg ctgggcaatt ggtcgattgg ccaagatgaa
     1561 gatggttatt ttaaagaagg agctatcggc tatgctttag aattttttgc ggacacgctt
     1621 agtttagata aagataaatt ttatattact atttttaaag gagaaaataa tataaaaagg
     1681 gatgaggaat ctttaaaaat ttggcaaaaa aatggcattc caaaagaacg gatatttgaa
     1741 tttggcaagg ataatttttg gggaccagtg agtgaaactg gtccttgtgg gccttgctcc
     1801 gagattcact atgacagggg agaagaattt ggctgtggca gacctgattg tgggccgaat
     1861 tgcaaaagat gcaagcgttt tgtggaattg tggaatttgg tttttatgga atattggaaa
     1921 gacgaaaaag gagattatca aaaacttcct cagacaaatg tagatactgg tattggcttt
     1981 gagcggttat tatcggtatt agagagaaaa tcctcggcct atgaaactga tttgctttta
     2041 ccggttattc aagaaattga aaagttatct cccaacgctt ataagttgaa ccctaaggcc
     2101 tgtcgtatta ttgctgatca tctgcgggga gcttgttttt taattagcga gggaataatt
     2161 ccaggcaatg tagatagagg atatatatta aggcgtctta ttagaagaac agttcgttat
     2221 tctcgaattt tgaatttaaa agatttgtgg tataaaaacc cagtagaaaa aattattaca
     2281 aaatatagtg ttgtttatcc tgagttaaaa agcaatgagg agaaaatttt aacagtgtgg
     2341 cagaatgaag aagaaaaatt ttcaaaggca ctgtcacgtg gtttaaaaga atttgaaaaa
     2401 ttaattgaaa gggaaagaag gcaagggaaa aaccttattt ctggcagaga ggcatttaat
     2461 ttgtatgaga gttatggatt ccctttggaa cttactgaag aaatggcaaa agaaaaagga
     2521 attattgttg ataaattagg atttaaaaaa tctttaaagg aacatcagaa aatatctcgt
     2581 tttggcgctg aaaaaaaatt cggtggtcat ggaatcattc aaaatccaaa attcaaatct
     2641 caaaattcaa aagttgttca acttcacact gctacccatc ttttgcaatc agctttgcgt
     2701 caagtattgg gtcctgaagt taaacaaatg ggttctgata ttaatgaaga acgactaagg
     2761 tttgattttt cttttcccag aaaattaact aaagaagaaa ttcaaaggat tgagaacttg
     2821 gtaaatcaaa agattaaaga aaatttgaaa gtagaaagag aagaaatgaa actagaagat
     2881 gctttatcct ctggcgcttt agcttttttt agagagcaat atccagaaaa ggtaagtgta
     2941 tataagattt ttaatccaaa aaacaatgaa gtgttttcaa aagaaatttg tgctgggcca
     3001 catattgaaa gaaccggtgt cttgggtaaa tttaaaatca aaaaagaaga aagttcgggc
     3061 gctggtgtgc gacgtatcaa ggcaa
//
LOCUS       DAOTQD010000047         2968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_26048_length_2968_cov_9.326124, whole genome shotgun sequence.
ACCESSION   DAOTQD010000047 DAOTQD010000000
VERSION     DAOTQD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26048_length_2968_cov_9.326124"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1798
                     /locus_tag="P5570_02230"
     CDS             2..1798
                     /locus_tag="P5570_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR39551.1"
                     /translation="MAFFNKTKKEADKKIEKGWEVEKKEKILNLFDIIAPPSLEIKQN
                     HLKVGNKFVRTYFVLTWPSSVVTNWLSPIINFDRVFDISMMIYPIETSGILKQLQKKI
                     TEVEALIMEREKKGLIRDPELETAYRNIEDLRDKLILAQEKMFKFGLYFTIYADEESD
                     LERIEKEMRQILESQLIYIKPAIFRQKEGFNSSSPLLMDELNISTTMNTSPLSASFPF
                     VSFELTSDRGILYGINRHNNSLILFDRFSLENGNVTIFAKSGSGKSYFEKLEILRSLT
                     QGIDFIVIDPENEYKYLAETVGGAYFNISLTSSHHINPLDLPPPVEGESKEGLFHSNI
                     INLVGLLRIMLGGLTPEEDAIIDNAINLTYLSQDITPQSDFYNQTPPLLSDLQKVLEG
                     MQGAESLALRLEKYTKGAFSGLLNHHTNVNLDNRLVVFSIRDLEDELRPIGMFLILHY
                     IWNIVRSKLKKRILVIDEAWWMMQYEDAASFLYGIAKRARKYYLGVSTITQDVDDFMR
                     SRYGPAIIANSSLQILFKQSPATIDVVMETFHLTQEEKNLLLQAEVGEGLFFAGSKHV
                     AMKVVASYSEDQIITTDPEKILEIRRAKEMFE"
     gene            complement(2153..2938)
                     /locus_tag="P5570_02235"
     CDS             complement(2153..2938)
                     /locus_tag="P5570_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRR39552.1"
                     /translation="MNIFLLAFQEILYRPILNLLVFFYNLPIVDFGIAIIILTILIRL
                     ILWPLNSKSITSQRETQIKTQLLQEKMKEIKKKYKNDPTRQNEEMMKLWKENKFNPFA
                     SFLPMIIQLIIVIALYQVLQNVLAPNGLDLLYGFVPRPEEIDPAFLGILDLSQKSWIV
                     AILAGVSQYFSSKMIFSFQKESQDKKLKKENKKKAKESSEEDRTEEMQKKMQKMMESQ
                     TTYFLPALTIFIGAALPSALPLYWLVSTLLGIIQQKLIYKKKS"
BASE COUNT          947 a          516 c          536 g          969 t
ORIGIN      
        1 tatggcgttt tttaacaaaa ctaaaaaaga agcagataaa aaaattgaaa agggctggga
       61 ggtggaaaaa aaggaaaaaa ttctcaatct ttttgatatt attgctccgc ctagtttaga
      121 aataaaacaa aatcacttaa aagtaggtaa taaatttgtc cgcacctact ttgttttaac
      181 ctggcctagt tctgttgtta ctaattggct ttctcctatt attaactttg atcgtgtttt
      241 tgatatttca atgatgattt atccaataga aaccagcggc attttaaaac aacttcaaaa
      301 gaaaattact gaggttgagg cccttattat ggaaagagaa aaaaagggat taattcgaga
      361 cccagagcta gaaactgcct atcgcaatat agaagacctg cgtgataagc tcatcttagc
      421 tcaagaaaaa atgtttaagt ttgggcttta ttttaccatc tatgctgatg aggaaagtga
      481 tcttgaaaga attgaaaaag aaatgcgcca aattttggaa tctcaactta tttatatcaa
      541 gcccgctatt tttcggcaaa aagaaggatt taattcatct tcgcctcttt taatggatga
      601 acttaatatt tctaccacaa tgaatacctc tcccttgtct gccagttttc cctttgtttc
      661 gtttgaactt acctctgaca ggggaatact ttatggtatc aaccgtcaca ataattccct
      721 tattttgttt gacaggtttt ctttggagaa tggaaacgtc acaatttttg caaaatcggg
      781 ctctggaaaa tcatattttg aaaagttgga aattttacgt tctttgaccc aggggataga
      841 ttttattgta attgatcctg aaaatgaata taagtattta gcggaaactg tgggtggcgc
      901 atattttaat atttctctaa catcatctca tcatattaat ccacttgatc ttccgccacc
      961 tgttgagggc gaatcaaaag aaggactttt tcattcaaat ataataaacc tggtagggct
     1021 tttaagaata atgttaggcg gtttaacgcc ggaagaagat gctattattg ataacgctat
     1081 taaccttact tatctttctc aagatatcac tcctcaatct gatttttata atcaaacccc
     1141 tcctttgctc tcagatttgc aaaaggtgct cgagggaatg caaggcgcag aaagtttagc
     1201 cctgcgatta gaaaagtaca caaaaggagc tttttctgga cttttaaacc atcataccaa
     1261 tgttaatcta gataatcgat tagttgtttt ttctattcgt gacttggaag atgaattaag
     1321 accaatagga atgtttttaa tattgcatta tatttggaat attgtccgtt caaaactaaa
     1381 aaaacgaata ttggttattg atgaggcctg gtggatgatg caatacgaag acgctgcttc
     1441 tttcctttat ggaattgcta aaagagcacg taagtattat ttgggcgtat caaccattac
     1501 tcaagacgtt gatgatttta tgaggtctcg atatggccca gctattattg ctaactcttc
     1561 tttgcaaatt ctgtttaagc aatcaccagc tactattgat gttgttatgg aaacttttca
     1621 tttaactcaa gaagaaaaaa atctgctttt gcaagcagaa gtaggcgaag gactcttttt
     1681 tgccggatca aaacacgtag caatgaaggt cgtggcttct tacagtgaag atcaaattat
     1741 tactacagat cctgaaaaaa tcttagagat cagaagagca aaagaaatgt ttgagtaaaa
     1801 ataatttagt tgggttcaaa agtttaatgt aacattacct tcttgtcatc gcgagcgagg
     1861 tcaaagaccg agcgtggcga tcccgttgta ttgttgcggg cttcgcccga actcaaagaa
     1921 gatgcgtctt cttttcaacg agctcaccct gttcgctcga gattgcttcg ctgcggctcg
     1981 caatgacagt aagtggataa ttgtcattgc gagcccgaag ggcgtggcaa tcccgtggca
     2041 cgtccacaaa catactctct gggattgctt cggtcggcta acgcctccct cgcaatgaca
     2101 acgaagaccg agcgatctcg tgacattgtt gcgttaaaaa cttgaatcta ctttaacttt
     2161 tctttttata aatcaatttt tgctgaataa ttcctaataa agtagaaaca agccaatata
     2221 ggggtagggc agagggcagg gcagcgccaa taaaaatagt taatgctggc aaaaaatagg
     2281 ttgtttgact ttccatcatt ttttgcatct ttttttgcat ttcttctgtt ctgtcttctt
     2341 cactgctttc tttagctttt ttcttgttct ctttttttag ctttttgtct tggctctcct
     2401 tttgaaaaga aaaaatcatt tttgaagaaa aatactggga aacaccagcc aagattgcca
     2461 caatccaact cttttgtgaa agatctaaaa ttcctaaaaa tgcagggtct atctcttctg
     2521 gacgcggcac aaaaccataa agcaaatcta aaccattggg cgctaaaaca ttttgtaaaa
     2581 cctgataaag agcaattaca ataattaatt gaataatcat tggcaaaaaa gaagcaaatg
     2641 gattaaactt gttttctttc caaagcttca tcatttcttc attttgacga gttgggtcat
     2701 tcttatattt ttttttaatt tccttcattt tttcttgaag cagttgggtt tttatttgag
     2761 tttctctttg gctagtaata gattttgaat ttaaaggcca taaaattaat ctaattaaaa
     2821 tagttaaaat aataatagca attccaaaat caacaattgg cagattataa aaaaacacca
     2881 ataaattaag aattggccga tataatattt cttgaaatgc aagaagaaaa atgttcataa
     2941 taagttaaaa aatcgagccc cgcagcag
//
LOCUS       DAOTQD010000048         2871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_27154_length_2871_cov_8.208807, whole genome shotgun sequence.
ACCESSION   DAOTQD010000048 DAOTQD010000000
VERSION     DAOTQD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27154_length_2871_cov_8.208807"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1969
                     /locus_tag="P5570_02240"
     CDS             188..1969
                     /locus_tag="P5570_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39553.1"
                     /translation="MTKQKLNSMNKESGMVQFQILIYIVAIALLISFLLASVQSILET
                     LSKGLVVSAVEWVTGIDLAKLIPNSLTIILRSVQGLASWALQGHFVSVGVTDLAQMVG
                     WDIVRNLANIFLLLGLIFTAFGIMLGIKEYEAKKSLPILIIIALLINFTPVICGWIID
                     FSNYITDWLLKGAISDDTFVTMMESGDLPSGFAGTFMFSLFVIIAVITYFFFFILFLA
                     RYVFLLVFTIFSPIAFASKIFSPSEKAKKFFPSFLYWDTWWNQFLQWCTVGILASFFM
                     LIGNQIMLNMNDIQGTNPGGFMEAGIVYFFPILTLLIGFFSALEAAQGIAGLVTSNAM
                     KLGGFLAAGTKLAKPVARPLKDVGENLAMSGYEKLEKAPLGAFKREVTELNIGKKEEV
                     AQEIEKRKDKFIKMDATTRFGLADKKSPQDMSAVLMATAEKGDEEKILEGMNDDERKN
                     YEKQRNEILQYLEKVDYKGYKETVKRSPHWIADDKKRKETFASLPPSFIKEKIGKESF
                     KYLKENKEEAKEVASSFSVAQLKAITAIEDQETRQIAQETIEEALKKSLDENKLKPHM
                     KHYLTETTEGKSLFGLSQKENKSEKHS"
     gene            2054..2428
                     /locus_tag="P5570_02245"
     CDS             2054..2428
                     /locus_tag="P5570_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39554.1"
                     /translation="MVGQLKIGDLSVQEQNKIFSCIDIFWPMAEELKVPEEKIDKSEK
                     LTEQTLMGFLHPNECITEIKNLFGPSSKQGEEFIQKINFEIFHPLKEELAKMYNDFDS
                     ENKKNSSSSGPKTLAENLGFKK"
     gene            complement(2473..2667)
                     /locus_tag="P5570_02250"
     CDS             complement(2473..2667)
                     /locus_tag="P5570_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39555.1"
                     /translation="MILYILLGIAFFLLILLILGRPRKENIKEKKKANTTIISTIASV
                     IVGIILVIVGGMLAAIFGGK"
     gene            complement(2672..>2871)
                     /locus_tag="P5570_02255"
     CDS             complement(2672..>2871)
                     /locus_tag="P5570_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39556.1"
                     /translation="QLKETPEQQGEQQQGEQQEPAREEETSQQVEDEEQTEEQTEEQT
                     GGQTQPVSEKKKDSNYIVLEE"
BASE COUNT         1016 a          452 c          514 g          889 t
ORIGIN      
        1 ggggttgggg cagggacatt gggcaaatca ggataatcta cctcatcagc aaaaaagaac
       61 aagggcttgg ctaaaattaa aaaaagaagt aaaataaaaa taaagaaaac tttttttgac
      121 tgcataaatt tgttttttta ttataacatg ttataataaa aaaacaaaca aagattagaa
      181 taaaaatatg acaaaacaaa aattaaattc aatgaataaa gaaagcggga tggttcagtt
      241 tcagattctt atttatattg ttgcaattgc tcttttaatt tcttttttgt tagcgagtgt
      301 tcaaagcata ctagaaaccc tctcaaaggg actagtagta agtgcagtag aatgggtaac
      361 aggaatagat ctggcaaaat taataccaaa ttctttaact ataatattaa gatctgttca
      421 aggattagct agttgggcat tacaaggaca ttttgtttca gtgggcgtaa ctgatcttgc
      481 tcaaatggtg gggtgggata ttgtaaggaa tcttgccaat atttttttat tactaggatt
      541 aatctttact gcttttggaa ttatgctggg gataaaagaa tatgaagcca aaaaaagcct
      601 gcccatttta atcattattg ctcttttaat taactttact ccagtaatct gtggctggat
      661 tattgatttt tcaaactata tcactgattg gcttttgaag ggggcaattt cagatgacac
      721 ctttgtgaca atgatggaat caggagacct accaagcggt tttgcaggca cctttatgtt
      781 ttctcttttt gtaattattg cagttattac ttattttttc ttttttattc tgtttttagc
      841 gaggtatgtt tttcttttgg tttttaccat tttttcacca attgcctttg ccagtaaaat
      901 tttttcgcct tcagaaaaag caaaaaaatt ctttcccagt tttttatatt gggatacatg
      961 gtggaatcaa tttttacagt ggtgcacagt gggaatcttg gcaagtttct ttatgttaat
     1021 cggcaaccag ataatgctaa atatgaatga tatccaagga accaatcctg gaggatttat
     1081 ggaagcgggt attgtttatt ttttccctat tttaaccttg cttattggat ttttttcggc
     1141 tttagaggcc gctcagggaa ttgcaggact tgttacctct aacgcaatga agttaggagg
     1201 atttttagca gcaggaacaa aattagcaaa accagtagca agaccgttaa aagatgtggg
     1261 tgaaaatctt gcgatgtcag gatatgaaaa gcttgaaaaa gcccctctcg gagctttcaa
     1321 aagagaagtc actgaattaa acatagggaa aaaagaagag gttgctcaag aaatcgaaaa
     1381 aaggaaagat aaatttataa aaatggatgc cacaacccgt tttgggttag cagacaaaaa
     1441 aagtccccaa gatatgagcg ctgtattaat ggcaacagca gaaaaaggag atgaggaaaa
     1501 aattctagaa ggaatgaacg atgacgagag aaaaaattat gaaaaacaaa gaaatgaaat
     1561 ccttcagtat ttagaaaagg tggactataa aggatacaaa gaaacagtaa aaagatctcc
     1621 tcattggata gctgatgaca aaaaaagaaa agaaaccttt gcaagtcttc ctccctcgtt
     1681 tattaaagaa aaaatcggca aagaaagctt taaatatctt aaagaaaata aagaagaagc
     1741 caaagaagtt gcaagttctt ttagcgtcgc ccaattaaaa gcgattactg caatcgaaga
     1801 ccaagagaca agacaaattg cacaagaaac cattgaagag gcgcttaaaa aatcattaga
     1861 tgaaaacaaa ctaaagccac atatgaaaca ttatctaact gaaacaaccg aaggaaaatc
     1921 tctttttggc ttgagccaaa aagaaaacaa gtccgaaaaa cattcctaga tcttttaaga
     1981 tttctccttg ttttactaga gatgacaaaa aagaaaaata aatatttatc ttattttaat
     2041 aaataataaa aatatggttg gtcaattaaa aattggcgat ctatctgtcc aagaacaaaa
     2101 taaaatattt tcttgtatag atatattttg gccaatggca gaggaattaa aagttcctga
     2161 agaaaaaata gacaaaagtg aaaaactaac agagcaaacc ctgatggggt ttttgcatcc
     2221 aaatgagtgt ataacagaga taaaaaactt atttggcccc tcttcaaaac aaggagagga
     2281 atttattcaa aagatcaatt ttgaaatttt tcatccttta aaagaagaac ttgcaaaaat
     2341 gtataatgac tttgattcag aaaacaaaaa aaattcctct tcgtcaggtc caaaaactct
     2401 tgctgaaaat cttggattta aaaaataaaa aagagaggac acggttaata aacatgttct
     2461 ctctgttcaa agttatttgc ctccaaaaat tgcggcaagc atgccgccaa ctattactaa
     2521 aattatccca acgataaccg aagctatcgt ggaaataatt gtagtgtttg ccttcttttt
     2581 ttcttttata ttctccttcc ttggtctccc aagaattaaa agaattaaaa gaaaaaaagc
     2641 gatccctaaa agaatatata aaatcattta tctactcctc caagactata tagttgctat
     2701 cttttttctt ttctgatact ggctgcgttt gtcctccagt ttgctcttca gtttgctctt
     2761 cagtttgctc ttcgtcctct acttgctggc ttgtctcttc ttctctagct ggttcttgct
     2821 gttctccttg ctgctgttct ccttgctgct ctggcgtttc ttttaactgg c
//
LOCUS       DAOTQD010000049         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_27281_length_2862_cov_10.444247, whole genome shotgun
            sequence.
ACCESSION   DAOTQD010000049 DAOTQD010000000
VERSION     DAOTQD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27281_length_2862_cov_10.444247"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="P5570_02260"
     CDS             complement(<1..1532)
                     /locus_tag="P5570_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39557.1"
                     /translation="MTNLLKSYDRTKALEPRSGIQGITQKYLDFIDSWERIGRYEDKD
                     GKKIDILVIKLKKEVSLYRARSAQRNFVADYLQGKLGTTTEKDAALVAFVSLDSEDWR
                     FSLVKIDYRFEETSTGKVKVREEFTPSKRWSFLVGANEKSHTAQSRFLPLLENDSHRP
                     TLEDLEIAFDVEVVTKEFFDAYRYTLKEIVVKSLSRYDAPYEKKYSFAQLLLSRIMFL
                     YFLQRKGWFKWKDYTPDKSYVKNLWLKYKEWRQEKEIKDTFYSQWLSSLFFGAFNKKS
                     YCFHKNLSEDIRESFSIMPFLNGGLFSKTELDELGFDLPDSAFEWLFEPDSSDLKKGF
                     LETFNFTIDESLPIDIEVAVDPEMLGKVYESLIAEEERGESGIFYTPRIEIDFMCRIS
                     LVEYLANETNILKDKIIDLVFNPHDKVSLFNKNELAHVKDKLDKLKVVDPAVGSASFL
                     VGMMNILVELHQELTRKLENRKENLFTLKQKIILENLYGVDAKDWAVMVGELRLWLSL
                     II"
     gene            complement(1603..1950)
                     /locus_tag="P5570_02265"
     CDS             complement(1603..1950)
                     /locus_tag="P5570_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRR39558.1"
                     /translation="MREIRDYLIDIKTECEYLISKSENLNYENFITNEELKRAFVRSL
                     EIIGEASKHIPKELKRKYSQIRWKSVIGMRNLLIHEYFGVDYRVLWKAIKERIPELYE
                     VIKNMIKEANKNE"
     gene            complement(1947..2240)
                     /locus_tag="P5570_02270"
     CDS             complement(1947..2240)
                     /locus_tag="P5570_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRR39559.1"
                     /translation="MTKIDEIKEKLRELKPLLQEKYKVSEIGIFGSYVRGEQKKRSDL
                     DVLVDFYEVPDLWSYIELQDFLLKKLKVKVDLVMKSALKPYIGKIILQEVVYI"
     gene            complement(2227..>2862)
                     /locus_tag="P5570_02275"
     CDS             complement(2227..>2862)
                     /locus_tag="P5570_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRR39560.1"
                     /translation="KRLPKKARSAKVSSDNLRDFASTNSLLTFFRKGKLMKFFLSNKE
                     HTVELDFLTSAKILESAEDEKRVKLPLENYYEFLDKNKSAFFNATIEEIIETRRRGGR
                     STFDELLKILKATQKNSKQLTEDQEEYLKKVINRIEEGALPKQTVKKALNALNKLGKD
                     IQNPLKVIGTLQREISPTFLKSHYAEPSAIKEGKREVILSLYLEGNEYDKN"
BASE COUNT          867 a          550 c          394 g         1051 t
ORIGIN      
        1 tctataatta aagagagcca taatctcaat tcaccaacca tcacagccca atccttagca
       61 tcaacgccat agaggttttc aagaatgatt ttttgcttca aggtaaataa attttctttt
      121 ctattttcaa gcttccttgt taattcctga tggagttcaa caaggatatt catcattcct
      181 acaagaaatg aggcagaccc aacagcaggg tcaacaacct ttaatttatc aagtttatct
      241 ttgacgtgtg caagctcatt tttattaaat aacgaaactt tatcatgcgg attaaaaaca
      301 agatcaataa tcttatcttt caggatgttt gtttcatttg caagatattc aacaagagag
      361 attctgcaca taaagtcaat ttcaattctt ggggtataga aaatgcctga ttctcctctt
      421 tcttcctcgg caatcaaaga ctcataaact tttccgagca tttcagggtc aactgcaact
      481 tcaatatcta ttggtaaaga ttcgtcaatg gtaaaattaa atgtttcaag gaatcccttt
      541 ttcaggtcgg atgagtctgg ttcaaacaac cattcaaaag cactatcagg aagatcaaaa
      601 cccagttcat ctaattcagt tttcgagaaa agaccaccat ttaaaaaggg cataatacta
      661 aatgattccc taatatcctc tgacaaattt ttgtggaaac aatatgattt cttattaaat
      721 gctccaaaga aaaggctgga aagccattga gaataaaaag tgtcttttat ttccttttct
      781 tgtctccact ctttatattt cagccaaaga ttttttacat agcttttatc cggggtataa
      841 tctttccatt taaaccatcc cttcctttgc aggaaataaa gaaacataat tcttgataac
      901 aataactgag caaaagaata tttcttttca taaggagcat cataccgaga gagagactta
      961 acaacaattt cttttaaagt atacctgtat gcatcaaaga actctttagt aacaacctca
     1021 acatcaaatg ctatttcaag atcttcaaga gtgggacgat gagaatcatt ttcaagcaaa
     1081 gggagaaaac ggctctgtgc agtatggctt ttttcatttg ctcctacaag aaaagaccat
     1141 ctctttgaag gtgtgaattc ttctcttact ttaacttttc cagtagaggt ttcctcaaag
     1201 cgataatcta tctttacaag ggaaaatcgc caatcttcac tatcaagtga cacaaaggct
     1261 acaagggcag catctttctc agtagttgtt ccaagtttcc cctgtaaata atctgcaaca
     1321 aaatttctct gtgcagaacg ggcacgatat agagaaactt ctttttttaa ttttattact
     1381 aaaatatcta tctttttgcc atctttatct tcatacctac caattctctc ccatgaatct
     1441 ataaaatcaa gatatttttg agttatccct tgaattccac tccttggttc aagggctttt
     1501 gttctatcgt aacttttcaa aagattagtc ataaaattaa taaagttttg tttattaaag
     1561 gaattttcaa aaacttcaat tattaacctt ttggcttgtt ctctattcat ttttgtttgc
     1621 ttctttaatc atatttttga tgacttcata taattctggt attctttctt taatggcttt
     1681 ccacaaaacc ctgtaatcaa ctccaaaata ttcatgaatc aaaaggtttc tcatccctat
     1741 aacgcttttc catctaattt gagaatattt tctttttaac tctttaggaa tatgcttact
     1801 ggcttctcca attatttcaa ggcttcttac aaatgctctt ttcagttctt catttgtgat
     1861 aaaattctca taatttaaat tctcactttt gcttatcaag tactcacatt ctgtttttat
     1921 gtctatcaaa tagtctctta tttctctcat atataaacaa cctcttgcaa gataatcttc
     1981 cctatatacg gcttaagcgc agatttcata acaagatcaa ctttaacttt taattttttt
     2041 aacaaaaaat cttgaagttc aatgtatgac cacagatcag gaacttcata aaaatcaaca
     2101 aggacatcaa gatcacttct ctttttctgc tcccctctta cataagaacc gaatattcct
     2161 atctcgctaa ctttgtattt ttcttgaagc agaggtttca gttctcttaa tttttctttt
     2221 atctcatcaa tttttgtcat attcattacc ctccaaataa agtgataaaa tgacttccct
     2281 ttttccttct tttatagcag agggttctgc gtagtggctt ttaagaaagg ttggtgaaat
     2341 ctctctttgt agtgttccta taacttttaa tggattttgt atgtcttttc caagtttatt
     2401 taatgcatta agggccttct ttaccgtttg tttaggtaaa gctccttcct ctatccggtt
     2461 tatcactttt ttaagatatt cttcttggtc ttcggttaat tgtttgctat tcttttgagt
     2521 ggctttcagg atttttaaaa gctcatcaaa agtgcttcta ccgccacgcc ttcgcgtttc
     2581 aattatttcc tcaatagttg cattgaaaaa agcactcttg ttcttatcaa gaaattcata
     2641 gtaattttca agaggtagtt ttactctttt ttcatcttct gcactttcaa gaatttttgc
     2701 agaggttaaa aaatcaagtt ctacagtatg ttctttattt gataagaaga acttcataag
     2761 ttttcctttt ctgaaaaatg tgagcaatga gttggtagag gcaaaatctc ttagattatc
     2821 agaagatacc tttgccgaac gagccttctt cggaagtcgc tt
//
LOCUS       DAOTQD010000050         2694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_29541_length_2694_cov_7.463054, whole genome shotgun sequence.
ACCESSION   DAOTQD010000050 DAOTQD010000000
VERSION     DAOTQD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29541_length_2694_cov_7.463054"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..509)
                     /gene="rplX"
                     /locus_tag="P5570_02280"
     CDS             complement(120..509)
                     /gene="rplX"
                     /locus_tag="P5570_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRR39561.1"
                     /translation="MKIKKDDQVLIISGKDRGRKGKVLKSLPKEGKIIVEKINIKRKH
                     LRPKKQGEKGQVVEIPAPIDVSNVKIICPKCGKPARVGYKNISNFKSQTEQSEMKSEE
                     KRDFSDHKNPKQKKLKVRICKKCGQEL"
     gene            complement(569..940)
                     /gene="rplN"
                     /locus_tag="P5570_02285"
     CDS             complement(569..940)
                     /gene="rplN"
                     /locus_tag="P5570_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRR39562.1"
                     /translation="MIQPRTMLKVVDNTGAKTIQCFKVFGGSKRRYAQIGDIIMGSVK
                     EAEPRRTIKKKDIVRAVIVRQRRPFRLPSGAYLRFDDNAVVILDGKTKNPKGNRVFGP
                     IPRILKEKGFDKIVTMAKAIV"
     gene            complement(1026..1307)
                     /gene="rpsQ"
                     /locus_tag="P5570_02290"
     CDS             complement(1026..1307)
                     /gene="rpsQ"
                     /locus_tag="P5570_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013755881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRR39563.1"
                     /translation="MKKQLQGEVISNKMQKTVVVCVESWAYHPKYKKRYKVHKNYKAH
                     NLIGLDPKDSKHNQEELKLGDRVIIEETRPISKEKKWRVIGKVNSKIKN"
     gene            complement(1350..1541)
                     /gene="rpmC"
                     /locus_tag="P5570_02295"
     CDS             complement(1350..1541)
                     /gene="rpmC"
                     /locus_tag="P5570_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRR39564.1"
                     /translation="MEMAELKKLSKKELENLLLEKRERVWQLRFGLTQKKVKNTREIK
                     EIKKDIARILTFLRIASQD"
     gene            complement(1600..2004)
                     /gene="rplP"
                     /locus_tag="P5570_02300"
     CDS             complement(1600..2004)
                     /gene="rplP"
                     /locus_tag="P5570_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRR39565.1"
                     /translation="MLQPKRVKHRKMMKGRSKGIERRGTEVSFGSFGLKAMGSKWITS
                     NQIESARRVLLRKLKGKGKIWIRIFPAKPVTFKGSEVGMGGGKGAVDHYVFPIRPGRI
                     IFEIDGVPREVAIDALNEAGRKLPIRTKIIET"
     gene            complement(2028..2657)
                     /gene="rpsC"
                     /locus_tag="P5570_02305"
     CDS             complement(2028..2657)
                     /gene="rpsC"
                     /locus_tag="P5570_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRR39566.1"
                     /translation="MAHKVHPKIFRVGITEEWSSMWFSDKKYQKNLKEDTKIREFLAK
                     EFEKGLIEEVKIERLANKINIIIKTARPGLLIGKGGSGAENLSKKITEFIKGKEVKID
                     IEEIKEPSTSATIVAEQIAVDFERRVPYRRSVKRAMERILQRKEIEGVKIKVKGRLNG
                     TEIARSETFKKGKLPLQTIRANIDYGTARAHCTYGVVGIKVWLYKGEKI"
BASE COUNT          713 a          534 c          303 g         1144 t
ORIGIN      
        1 gctcgcttcg ctcgcgatat tcattgtaac aataaatatc tataaatatc tataagtatc
       61 tacaagtatc tacaagtatc tacaagtatc tattattgtt ttgcattttg aattattttt
      121 tatagttctt gaccgcattt tttgcatatt ctaactttta attttttttg ttttggattt
      181 ttatgatcgc taaaatctcg tttttcctcg cttttcatct cgctttgctc ggtttggctt
      241 ttaaaatttg agatgttttt gtatcctacc ctagctggct ttccacattt tggacaaata
      301 attttaacat tggaaacatc aataggagca ggaatctcaa ctacttggcc tttttctcct
      361 tgctttttgg gacggagatg ttttctttta atatttattt tttcaacgat aatttttcct
      421 tcctttggaa gggattttaa cacttttcct tttcttccgc gatctttccc actaataatt
      481 agcacttgat catctttttt aattttcaca aatttttacg aatctatttg cgaatattcg
      541 caaataaaat tctggttaat taacaatttt acactatggc ttttgccatt gttactattt
      601 tatcaaatcc tttttctttt aaaatccttg ggataggacc aaaaactcta ttacctttag
      661 gattctttgt tttgccatct aaaataacta ctgcattatc atcaaatctt aaatacgcac
      721 cgcttggaag cctaaaaggc cttctttggc gtacaataac tgcccgtaca atatcttttt
      781 tcttaattgt tcttcgtggc tctgcttctt ttactgaccc cataataata tctcctattt
      841 gggcatatcg tcttttagac ccaccaaaaa ccttaaaaca ctgaatggtt tttgctccag
      901 tattatctac aactttaagc attgttcgcg gttgaatcat attcgcttcg cttaaaatca
      961 aaatgcaaat atcaaaaaga aaaattaaaa gattcttttt gcattttaca ttttcatttt
     1021 gctttttaat ttttaatttt tgaattaact tttcctatca ctctccattt tttttctttt
     1081 gaaatcggcc ttgtttcttc aataataacc ctgtcgccca attttaattc ttcttgattg
     1141 tgcttagaat cctttgggtc cagcccaatt agattatggg ctttgtaatt tttatgaacc
     1201 ttatatcttt ttttatattt tggatgataa gcccaactct caacacaaac cacaacggtc
     1261 ttttgcattt tatttgaaat aacttcacct tgtaattgtt ttttcataaa ttttctatca
     1321 attatgaatt taatcaaact aatttgtaat tagtcctgac ttgcaattcg taggaacgta
     1381 agaattcttg ctatgtcttt ttttatttct ttaatttctc ttgtgttttt taccttcttt
     1441 tgagtcaatc caaaacgcaa ctgccaaact ctttctctct tttctaaaag aagattttct
     1501 aattcttttt ttgataattt ttttaattct gccatttcca tatttataaa gtatattgct
     1561 aaaatattac acaaataatt tttcagtatt gttttgcgtt tatgtctcta ttatctttgt
     1621 tctaataggt aatttgcggc cagcttcatt aagagcatca atcgctactt ccctgggtac
     1681 accatctatc tcaaaaatta ttctaccagg ccttatagga aacacataat ggtcaactgc
     1741 tcctttgcct cctcccattc ctacttcact acctttaaat gttactggtt ttgcaggaaa
     1801 aattcttatc caaatttttc cttttccttt taattttctt aaaagcactc ttcttgcaga
     1861 ttcaatttga ttagaagtaa tccatttact tcccattgct tttaaaccaa aactcccaaa
     1921 ggaaacttcg gtgccccttc tttctatccc cttacttcgt cctttcatca tctttcgatg
     1981 tttaacacgt tttggttgaa gcataattta gcttattaat atgttaatta tattttttct
     2041 cctttataca gccatacctt tatccctact actccataag tacaatgagc tcttgctgtc
     2101 ccataatcaa tatttgctct aatggtttga agaggcaatt tgcctttttt aaaggtttcg
     2161 cttcttgcaa tctctgtacc atttaaacga ccttttactt taattttaac tccttctatc
     2221 tctttccttt gtaaaattcg ttccattgcc ctttttactg atcgacgata aggcactctt
     2281 ctctcaaaat caactgcaat ctgttctgca acaatagtag cactggtaga tggttctttt
     2341 atctcttcaa tatcaatttt gacttctttt ccttttataa attcagtaat tttttttgaa
     2401 agattttctg caccactgcc acctttgcca attaacaaac caggtcgtgc tgtttttata
     2461 ataatattta ttttattagc aagacgttca atctttactt cttctattaa tcctttctca
     2521 aactcctttg ctaaaaactc cctaattttt gtatcttctt ttaggttttt ttgatatttt
     2581 ttgtcagaaa accacattga actccattct tcagttattc ctactctaaa aatttttgga
     2641 tgaactttat gagccatatt aatataattt ctaatttcta atttctaatt ttta
//
LOCUS       DAOTQD010000051         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_33039_length_2474_cov_7.431997, whole genome shotgun sequence.
ACCESSION   DAOTQD010000051 DAOTQD010000000
VERSION     DAOTQD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33039_length_2474_cov_7.431997"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..927)
                     /gene="rpsB"
                     /locus_tag="P5570_02310"
     CDS             complement(169..927)
                     /gene="rpsB"
                     /locus_tag="P5570_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRR39567.1"
                     /translation="MEKKTQKETIKEKKEKTSLEEKSLKEKIDSFDDAEIKKMLEAGV
                     HFGHRHSKTYPKMAPFIYGSRNTIDLINPLKTKEYLEEALNFLKKQKEKKSLILFVGT
                     KISAKKLIKELAEELSMPYVNERWLGGTLTNFEVISKRINYLKEMEAKRDTGKLDDYK
                     KKERVKINQEIARIERKISGLKNLSHSPDVLFVIDIEEEKLAVKEANKKKIPIVGICD
                     TNGNPDMVDYPIPANDDAVSSLKYILDKVKEALK"
     gene            1095..1376
                     /gene="rpmE"
                     /locus_tag="P5570_02315"
     CDS             1095..1376
                     /gene="rpmE"
                     /locus_tag="P5570_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRR39568.1"
                     /translation="MKKDIHPPYFKNAVQHCACGAVFHIPSTQKETKTEACSQCHPFY
                     TGKAKIVDATGQVEKFKERIKKSEQLKVKTKNKKKTRKKKENGKKSRKV"
     gene            1351..2442
                     /gene="prfA"
                     /locus_tag="P5570_02320"
     CDS             1351..2442
                     /gene="prfA"
                     /locus_tag="P5570_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRR39569.1"
                     /translation="MEKNLEKFKEEYNNLQRRLSQLSELSLAINNKKSENKTISLNKK
                     NYGELSKQFSFLQSVIDQINKKEKIEKSIKENEELLKTEEDKEFQKLAEEEISKLKQE
                     KEKIEKEIERIIEENESDKSSTDAVIVEIRAGVGGEEASLFAKELFEMYQKFCQKKDW
                     HIKILSSHSTDLGGIKEISFEIKGKGCEDLKYESGVHRVQRIPETEKLGRVHTSTVSV
                     AVLEKPKEKEIEIRPQDLRIDTYRASGPGGQYVNRRDSAVRITHIPTGIQVASQSART
                     QIANRENAMSLLEAKLFQIKKEEEEKKLEASRKGQIGKAMRAEKIRTYNFPQDRITDH
                     RIKKSWKHINEILEGNLDELIEEVKKHNI"
BASE COUNT          952 a          346 c          367 g          809 t
ORIGIN      
        1 gccgaagttt tttgatttga tcaacattaa tagccataat taaaaaattt aaattgcaaa
       61 atacaaatat caaaatgtaa tgtataaatt aaaaaagaaa agaaagttaa aaaaataatt
      121 cttttttaca ttttagatta tcattttgat tttgattttg attttgattt attttaatgc
      181 ctcttttact ttatctaaaa tatattttaa tgatgatact gcatcatcat tggctggaat
      241 tggataatca accatatcag gatttccatt ggtgtcgcaa atacccacta ttggaatttt
      301 tttcttatta gcctctttta ccgctaattt ttcttcctcg atatctatca caaaaagaac
      361 atctggagaa tgcgacaaat tttttaatcc gctaattttt ctttctattc tggcaatttc
      421 ttgattgatc tttacccttt cctttttttt ataatcatct aatttgcctg tatctctttt
      481 tgcctccatt tcttttaaat aatttattct ttttgaaatt acttcaaaat tagttaatgt
      541 tccgcccaac caacgttcgt ttacatatgg cattgaaagt tcttcagcta attcttttat
      601 taatttttta gcggaaatct ttgttcctac aaataaaatt aatgattttt tttctttttg
      661 tttttttaaa aaattcaatg cttcttctaa atattctttt gtttttaagg gattaataag
      721 gtcaatggta tttcgtgacc cataaataaa gggggccatt ttgggataag tttttgaatg
      781 acgatgacca aaatgcacac ctgcttcaag cattttttta atctcagcat catcaaaaga
      841 atctattttc tcttttaatg atttttcttc taatgatgtt ttttcttttt tttcttttat
      901 tgtttctttt tgtgtctttt tttccataaa aataataaag acctaaaaaa caaaattaca
      961 tcttgcaatt ttgtttatca taaatatatt atcaaaaact cttgcaaatg tcaaaggaaa
     1021 agagtataat agaaaataat ttaaaatcat tcaagtatat ttgtacaagt atatttgtag
     1081 attaaatttg ttttatgaaa aaagatattc atccaccata ttttaaaaat gctgtgcaac
     1141 actgtgcttg tggtgcagtt tttcatattc cttcgactca gaaagaaaca aagacagagg
     1201 cctgttctca gtgtcatccg ttttatactg gtaaagcaaa aattgttgac gccactggcc
     1261 aagtagaaaa atttaaagaa aggataaaaa aatcagaaca attaaaagtg aaaacaaaaa
     1321 acaaaaagaa aacaagaaaa aagaaagaaa atggaaaaaa atctcgaaaa gtttaaagaa
     1381 gaatataata atttacaaag aaggctcagt caattgtctg agctttcttt ggctataaat
     1441 aataaaaaaa gcgaaaataa aacgatttca ttgaataaaa aaaattatgg cgaattatca
     1501 aaacaatttt cttttcttca atccgttatc gatcaaataa ataaaaaaga gaaaattgaa
     1561 aaatccataa aagagaatga agaattgcta aagactgaag aagataaaga gtttcaaaaa
     1621 ttggcagaag aagaaatatc aaaattaaaa caagaaaaag aaaaaataga aaaagaaata
     1681 gaaagaatta ttgaagaaaa tgaatccgac aagtcttcaa cagatgctgt aatagtagaa
     1741 attagggcag gtgttggagg cgaagaagca tctttgtttg caaaagaact ttttgagatg
     1801 tatcaaaaat tttgtcaaaa aaaagattgg cacataaaaa ttctttcttc gcactcaaca
     1861 gatcttggag gaatcaaaga aatttctttt gaaattaaag gaaagggctg tgaggattta
     1921 aaatatgaaa gtggagttca tcgcgtccag cgaattccag aaaccgaaaa attgggcaga
     1981 gtgcatactt caactgtttc agtagcagtt cttgaaaaac caaaagaaaa agaaattgaa
     2041 attagacctc aggacttgcg aattgatact tatcgggctt caggacccgg cggtcaatat
     2101 gttaatcgtc gagattcagc agttagaatt actcacattc caactggaat tcaagtggca
     2161 agccaaagcg ctagaactca aattgcaaat agagaaaacg caatgtctct tttggaagct
     2221 aagcttttcc aaattaagaa agaggaagaa gaaaaaaaat tagaagcatc tcgcaaaggt
     2281 caaattggaa aagcaatgcg ggcagaaaaa atcagaactt ataattttcc ccaagacaga
     2341 attactgatc atcgcatcaa aaaatcctgg aaacatatta atgaaatctt agaaggcaat
     2401 ttagatgaat taattgaaga agttaaaaaa cacaacattt aaaaaataac ggagaacagt
     2461 tctccgttat tttt
//
LOCUS       DAOTQD010000052         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_33998_length_2423_cov_9.988176, whole genome shotgun sequence.
ACCESSION   DAOTQD010000052 DAOTQD010000000
VERSION     DAOTQD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33998_length_2423_cov_9.988176"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..2004
                     /locus_tag="P5570_02325"
     CDS             346..2004
                     /locus_tag="P5570_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRR39570.1"
                     /translation="MFDYSILVGGAAGQGSRKAGLLIAKLLNKYGYRIFIYDDYQSLI
                     KGGHNFSVIRASEKEVLSHRENIDFLLALDKNTIEKHKKSLTKTSSIIYNSDEISGVN
                     GIGIPLKKITQEEGGIPIMENVALVAGFAKITGIDWQCLKKTLQEEFKEKSEINLKIA
                     KRAYNETRSLIKIKKLNQKPLPLLTGNEALALGAVKAGLNFYFAYPMTPASGILHYLA
                     EHSEKFNIAVLQLENEVAIINAAIGAAYAGARTMVGTSGGGFALMTEGLSLAAQSETP
                     VVIVESQRMSPATGVPTYTEQADLLFCLSAGHGDFVRFVIAPGDADEAFYWAGETLNL
                     SLRYQTPSILLIDKEVSESTFSFHPEIQKKVKIKEPLIWKNNKGEYQRYKITKNGISP
                     LAFPGQKDIVVKATSYEHDENGITVEEDEKMIKAMQDKRLRKFEEMKKTVERLEAVKV
                     YGKKNSTEAIIVWGSTKGPAKEAAERLGIKLIQLIVIEPFPEKQIKEALRGVKNIVLA
                     ETNALGQMEKVLNCYGITVNKKILKYNGRPFTAEEIIEKIKMKD"
BASE COUNT          810 a          384 c          505 g          724 t
ORIGIN      
        1 ttttctgttt tctctactgt tttctgattt ctgataactg atttctgttt agtgtttggt
       61 atttagtatt ttgtatttgt ttggaatttg gaatttggtg cttggaattt gtttgtattt
      121 tgatttatgc gtatgtattg cgcttaacgc agaaaaaaaa ttgccccttg ctttatgagg
      181 aataattata atataataca taataaaaat gcaaatataa tagatttata gatacttgta
      241 gatatttatt gttacaatga atatcgcgag cgaagcgagc aagtatctat caatatcgtg
      301 ccgaaggcac aagtatctat aaattttgct ttttgcttta ttactatgtt tgattattct
      361 attttggttg gaggggcggc tggccaaggt tcaaggaaag ccggacttct aatcgcaaaa
      421 cttttaaata aatacggcta tcgaattttt atctacgatg attatcaatc ccttatcaaa
      481 ggcgggcata atttttctgt gattagggct tcggaaaaag aagttctgag tcatcgggaa
      541 aatattgatt ttcttttagc cctggacaaa aataccattg aaaaacacaa aaaaagttta
      601 acaaaaacca gttcaattat ctataatagc gatgaaattt caggagtaaa cggcattggc
      661 attcccctta aaaaaattac tcaagaggaa ggaggaatac cgataatgga aaacgttgcg
      721 ttggtagcag gatttgctaa gattacaggc attgactggc aatgccttaa aaagaccctg
      781 caagaagaat ttaaagaaaa aagcgaaata aatttaaaaa ttgccaaacg ggcttataat
      841 gaaaccagat ctttaatcaa aattaaaaaa ctaaaccaaa aacctttgcc gcttttaact
      901 ggtaatgaag cactggcttt gggagcagtg aaagccggac ttaattttta ttttgcctat
      961 ccaatgactc cagccagcgg tattttacat tatttggcag aacatagtga aaaatttaac
     1021 atcgctgttt tgcaattaga aaatgaagtt gctattatta atgctgctat tggcgcggct
     1081 tacgccggag caaggaccat ggtggggaca tcaggtggcg gatttgctct aatgacagaa
     1141 ggattaagtt tggctgccca aagcgaaaca ccggtagtta ttgttgaaag tcaaagaatg
     1201 tcaccagcta ccggcgttcc tacttatacc gaacaggcag atttgctttt ctgtttaagt
     1261 gctgggcatg gtgattttgt gcggtttgtg atcgcgcccg gagacgctga tgaagcattt
     1321 tattgggcag gagaaacttt gaatctttct ttaagatacc aaacaccgtc aattctttta
     1381 atagataaag aggtttctga aagcactttt tcttttcacc cggaaattca aaaaaaagtg
     1441 aaaatcaaag aacccctgat atggaaaaac aacaaaggcg aatatcaaag atataaaatc
     1501 acaaaaaatg gtatttctcc cctggctttt cctggccaaa aagatattgt cgtaaaagca
     1561 accagttatg agcacgacga gaacggaatt accgttgagg aagatgaaaa aatgataaaa
     1621 gcgatgcaag acaagcgcct aagaaaattt gaagaaatga aaaaaacagt agaaaggtta
     1681 gaagccgtaa aagtttatgg gaagaaaaat tcaacagaag caataattgt ttggggatca
     1741 accaaaggcc cagcaaaaga agcagctgaa agattgggta ttaaattaat ccaattgatt
     1801 gtaattgaac catttcctga aaaacaaata aaagaagccc tgaggggtgt aaaaaatatt
     1861 gttttggcgg aaacaaatgc cttggggcaa atggaaaagg ttttgaactg ctatggtatc
     1921 acggttaata aaaagatttt aaaatataac ggccggccat ttacagcaga agaaataata
     1981 gaaaaaatca aaatgaaaga ttaaaatgta aaaagaaaat cttcttgtca ttgcgaggag
     2041 cgttcaaaga agattttatc ttcttttcaa cgcttcgccc cgtgcgaaat caaagatttc
     2101 ttacggggct cgcgtgagcg acgaagcaat cccattgtat gtttgtagac gtgctacgag
     2161 atcgccacgt cgtccactca ccttttacta aggtgaggga ctcctcgcga tgacaatggg
     2221 ggttgccctc cctcaccttc acaaaggtga ggggtcgcga tgacagtggt gaggggtggt
     2281 aatgacaaga aaaaattttt cttttttgaa ttttgcatta cattttgctt tttgattttt
     2341 gcattttgat ttttgcatta tttttatgtt tgatatcaac attcaaaata cttggtgtcc
     2401 gggatgtggc aattttggaa ttt
//
LOCUS       DAOTQD010000053         2261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_37275_length_2261_cov_11.506346, whole genome shotgun
            sequence.
ACCESSION   DAOTQD010000053 DAOTQD010000000
VERSION     DAOTQD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37275_length_2261_cov_11.506346"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..2176
                     /locus_tag="P5570_02330"
     CDS             107..2176
                     /locus_tag="P5570_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HRR39571.1"
                     /translation="MILPDYPIQSKESDKLKRAPLAQKVADLILAFKGSESFVVGVEG
                     AWGAGKTSFINMVLETLGSKVYFFVFNPWNFSDQTSLLKDFFSSFSNIEPITIKKDLK
                     DKLTGYAKKLSDYGFQGINLSIISFNPLQKLLNESLSDIRNGLNKSLGSLEKKIVIVI
                     DDIDRLDADETKLIFKLVKLTANFPNTVFLLAYDRNRVEKRLTIEKDGFEGGEYLKKI
                     VQVSFVLPEPDLEDLWQILFEDLDATIKAVYGEVNVDDKRWGNLFHAGLKNLFITIRD
                     IKRFISSLRLDWSIVSKDDITPVDFIGIEAIRVFAPQFYSAMSANKHLFTATDSLYVG
                     LSSRDDKSARETKYKELLELIPENLKQPIDEICKQLFPQLDFRTSYPHDWQQTWRKEL
                     RVCSEEKFDFYFRLGIPTGAVSEAELKAVIKTLNKQDNFLKNLKKFNEEKRLRKILSR
                     LIDYLDDIDKDKINTFILSLWQLEEEVDDKRFGMWDLEDIDTLVSRLSYHSIKQKVEK
                     GKRGAFIAELVKNSSVLYPAVKFAGLVVHEFEEKNKKPSEETLLEQSEIENLKSAISG
                     KFNEIDKNNLKDNKHLAYLLFRWKENAGEEAVKSFITELLKNKSDTATFLSAFVSDVF
                     SQGIGDYVSTKKRELNKKSIEALYQISEVEKIVNNITDEDLKNFNEQQKEAIELFKKP
                     KKSDVFD"
BASE COUNT          795 a          332 c          461 g          673 t
ORIGIN      
        1 tatttatctc ggcgaaatta gaacgatgag agataaagat tttagagaaa tatggaaaaa
       61 ccgagtcggt aatatataaa ctattatctg tttaaaatat aagtaaatga tacttccgga
      121 ttatccaatt cagtcaaagg agagcgataa gctaaagcgc gcgccacttg ctcaaaaagt
      181 ggctgatttg attttggcgt tcaagggttc tgaaagtttt gttgtaggtg tagaaggcgc
      241 atggggagcg ggcaagacat cttttatcaa tatggttttg gaaacgctcg gtagcaaggt
      301 ttattttttt gtttttaacc cttggaattt ttctgaccaa acttcgctac taaaagattt
      361 ctttagctct ttttctaaca ttgaaccaat tacaatcaaa aaggatctaa aggataaact
      421 tacaggatac gcaaagaaac tatctgatta cggatttcaa ggaataaatt tatccatcat
      481 ttcttttaat cctttacaaa aactgctgaa tgaatcttta agtgatattc gcaacgggtt
      541 gaataaaagt ttgggcagtt tagaaaaaaa aattgttatc gttattgacg atatagacag
      601 attggacgcc gatgaaacaa aattgatttt taaactggta aaattgacag ctaattttcc
      661 taatacggtc tttcttttgg catatgacag aaacagagtt gagaaaagac tgacgataga
      721 aaaagacggc tttgagggcg gcgaatatct gaaaaagata gttcaagttt cttttgtgct
      781 tccagaaccg gacttggaag atttgtggca gattttgttt gaagatttgg acgcaactat
      841 caaagccgtt tatggcgagg taaacgttga tgacaaaaga tggggtaatt tatttcatgc
      901 gggcttgaag aatctattta ttactataag agatataaaa cgatttatta gttctttgag
      961 attagattgg tctattgtaa gcaaagacga tattacaccg gtagatttta ttggcattga
     1021 ggcgatccgt gtttttgcgc ctcaatttta cagcgcgatg agcgcaaaca aacatctttt
     1081 taccgcgaca gatagcttat atgttggctt gagttcaaga gatgataaaa gtgcaagaga
     1141 gacaaaatat aaagaattat tagagcttat ccccgagaat ttaaagcaac caatagacga
     1201 aatttgtaag caacttttcc ctcagcttga tttcaggaca tcttatccgc acgactggca
     1261 acaaacctgg cggaaggaat tgcgtgtttg ttctgaagaa aaatttgatt tctattttcg
     1321 cttaggtatt ccaacgggag cggtttccga ggcagagtta aaagcggtta taaagacact
     1381 gaataaacaa gataattttt taaaaaatct taaaaaattt aatgaagaaa agcgacttcg
     1441 taaaatttta agtcgcttaa ttgattatct tgatgatata gataaagaca aaatcaatac
     1501 ttttatttta agtctttggc aacttgagga agaagtggac gacaagcgtt ttggcatgtg
     1561 ggatttagag gacatagata cacttgtttc aaggctaagc tatcactcta taaaacaaaa
     1621 agtggaaaaa ggcaaaagag gtgcatttat tgcggaatta gttaagaata gtagtgttct
     1681 atatcccgca gttaaatttg ctggtttggt tgttcacgaa tttgaagaaa aaaacaaaaa
     1741 gccgagcgag gaaactttgc tcgaacaatc ggaaatagaa aatttgaaat cggctatttc
     1801 gggaaaattt aatgaaattg acaaaaataa tttgaaagac aataagcatt tagcatactt
     1861 gttatttcgt tggaaagaaa acgcaggcga agaagcggtc aaaagtttca ttactgaatt
     1921 gttgaaaaat aaatctgaca cggccacttt tttatccgct tttgtttcag atgtattttc
     1981 tcaaggtata ggcgattatg tgtcaacgaa aaagcgagaa ttaaataaaa aatcaattga
     2041 agctctttac caaatcagtg aagttgagaa aattgtaaat aatattactg acgaagattt
     2101 aaaaaacttt aacgagcaac aaaaagaagc tatagaacta tttaaaaaac caaagaaatc
     2161 tgatgtattt gattaagaca actaatccca cttaccactc ttttgaggcc ccgccgccgt
     2221 ccgaaaaaat cgctgggggt ttccagcagg cgggcaaaaa g
//
LOCUS       DAOTQD010000054         2168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_39471_length_2168_cov_7.182205, whole genome shotgun sequence.
ACCESSION   DAOTQD010000054 DAOTQD010000000
VERSION     DAOTQD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39471_length_2168_cov_7.182205"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /gene="gatA"
                     /locus_tag="P5570_02335"
     CDS             <1..1370
                     /gene="gatA"
                     /locus_tag="P5570_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRR39572.1"
                     /translation="DKDIHAFLTLTSEIALNQAKEAEKKFIEETHSEKIPLLCGIPCA
                     IKDNILVDGIRCTAASKILENYIAPYNATVIERLKKEGVVILGKTNMDEFAMGASTET
                     SAFGPTKNPLNENLVPGGSSGGSAAAVAADFCQFALGSDTGGSIRQPASFCGIIGLKP
                     TYGAVSRYGLMAMASSLDQIGPLTKNVEDAEIIFEAIKGKDPLDSTSVESKIENCRFS
                     SLLSAGARKLKIENLRVGIPKEYFVEGTEEGVRKEIEKGIKRFEELGAKIEKISLPHT
                     KYALATYYLIMPSEVSSNLARYDGIRYGLSNSKILKFDPQNSFEIYSKIRGEYFGKEV
                     KRRIVLGTFALSAGYYDEYYLKARAVREMIKKDFENAFQKIDLILAPVAPTVAFKIGE
                     KIDDPLKMYLSDIFTVGPSLAGVPALSLPCGFFNNMPVGLQIIGKPFGEEIIFEAAKE
                     FEKTV"
     gene            1409..1930
                     /locus_tag="P5570_02340"
     CDS             1409..1930
                     /locus_tag="P5570_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39573.1"
                     /translation="MSIEDFFLFFNTQLWENLESVLKPTAFIAIVIFIWAIVWALKKS
                     SWIYWYITADAQEFMRGAPVPLQKKAQQQWKKIKKRLKSRKEANWKLAVIEGGEIVDD
                     LLLRMGYKANGMKARLTLANEAQISNLDELTSAAEVYEGIIADPDYELSHQKAEETLR
                     AFEKFLKNYQYLN"
BASE COUNT          730 a          299 c          424 g          715 t
ORIGIN      
        1 aagacaaaga tattcatgct tttttaactt taacttcaga aattgcacta aatcaagcaa
       61 aagaagccga aaaaaaattt atagaagaaa ctcattcaga aaaaattcct cttctttgcg
      121 gaattccctg cgctataaaa gataatattt tagttgatgg tataaggtgt acggctgcgt
      181 caaaaattct tgaaaattat attgcacctt acaacgctac agtaattgaa agattaaaaa
      241 aagaaggagt agtgattttg ggaaaaacaa atatggatga atttgctatg ggtgcttcta
      301 ctgaaacttc tgcttttgga ccaacaaaga atcctcttaa tgaaaatctt gttccgggtg
      361 gatcctcagg gggatcagct gctgctgtgg cagctgattt ttgccaattt gcattgggtt
      421 ctgatactgg tggttcaatt cgtcagccag cttctttttg cggtataatt ggtttaaaac
      481 caacttatgg agcagtgtca agatatggct tgatggctat ggcttcctct cttgatcaaa
      541 ttggaccttt aacaaaaaat gttgaagatg ccgagataat atttgaggca ataaaaggaa
      601 aagatcctct tgattcaaca agtgttgaat caaaaattga aaattgcagg ttctcttcgc
      661 ttttatccgc aggagctcgg aaattgaaaa ttgaaaattt acgagttggt atcccaaagg
      721 aatattttgt tgaaggaaca gaggaaggtg taagaaaaga aattgaaaaa ggtataaaaa
      781 gatttgaaga gctaggagca aaaatagaaa aaatttctct tcctcatact aaatatgccc
      841 ttgcaactta ctatttaatt atgccctcag aggtttcatc aaatctagca cgatatgatg
      901 gtataaggta tggtttgtct aattctaaga ttttaaaatt tgatcctcaa aattcatttg
      961 agatttattc aaagattcgg ggagaatatt ttggcaaaga agtaaaaaga agaattgttc
     1021 ttggaacttt cgctctttca gctggctatt atgatgaata ttatttaaaa gcaagagcag
     1081 taagagagat gattaaaaaa gattttgaaa atgcttttca aaaaattgat ttaattttag
     1141 caccagttgc gccaactgtt gcttttaaga taggagaaaa aatagatgat cctttaaaaa
     1201 tgtatctttc tgatattttt acagttgggc caagtttagc tggcgtacca gctctttctt
     1261 tgccttgcgg tttttttaat aatatgccgg ttggtttgca aattattgga aaaccatttg
     1321 gagaagaaat tatttttgaa gcagcaaaag aatttgagaa aacagtttag aaattagatc
     1381 aattagaaat tagaaattta ttatctctat gtctattgaa gatttctttc ttttttttaa
     1441 tactcaattg tgggagaatc ttgaatctgt tttaaaaccg acggctttta ttgctattgt
     1501 gatttttata tgggcgattg tttgggcctt aaaaaagagt tcatggatat attggtatat
     1561 taccgctgat gctcaagaat ttatgagagg agcaccagtt cctctccaaa aaaaagcaca
     1621 gcagcaatgg aaaaaaatta aaaaacgttt aaaatctaga aaagaagcca attggaaatt
     1681 agctgttatt gaaggaggag aaatcgttga tgatcttctt ttaaggatgg gatacaaagc
     1741 caatggaatg aaagcaagat taactttggc taatgaggcc cagatttcca acttagatga
     1801 gttgacaagt gcagctgaag tgtatgaggg aattattgct gaccctgatt atgaattatc
     1861 tcatcaaaaa gcagaagaga cattgcgtgc ttttgaaaaa tttttaaaga attatcagta
     1921 tttaaattaa gttagattca aaaattaaat actaaacaga aggtggaaag tggtgaggga
     1981 ttgcttcgtt tactgatttc tgttttctct actgtcttct gttttctgac aactgatttc
     2041 tgtttggaat ttggaatttg tttgtatttt ggtgcttgtg atttggaatt tttacatttt
     2101 tacaaaggta acctttcaat ataatccagg aagtaataac tatatttgtc atcgcgaccc
     2161 ctcacctt
//
LOCUS       DAOTQD010000055         2107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_40955_length_2107_cov_8.606725, whole genome shotgun sequence.
ACCESSION   DAOTQD010000055 DAOTQD010000000
VERSION     DAOTQD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40955_length_2107_cov_8.606725"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..180)
                     /locus_tag="P5570_02345"
     CDS             complement(22..180)
                     /locus_tag="P5570_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39574.1"
                     /translation="MEKIIWEVEEMLKWASEEAPEEELIRKVVKIAREEDKGGLISRI
                     YLKGKRIY"
     gene            495..1895
                     /gene="cysS"
                     /locus_tag="P5570_02350"
     CDS             495..1895
                     /gene="cysS"
                     /locus_tag="P5570_02350"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRR39575.1"
                     /translation="MLKVYNTLSRKKEIFRPLHDKKVGLYTCGPTVYWFAHIGNLRTY
                     IFEDILKRTLEYNGYKVKHVMNITDVGHLTSDADTGEDKLEKGAKREKRSVWEIAEFY
                     TRVLKEDLKNLNIIPPNIWIKATDTIKEQIELIKVLEKKGFTYTIEDGVYFDTSKLKT
                     YGRLWPKNAGKEIKHRVEMVPGKKNPTDFALWKFSPKNVKRQMEWQSPWGIGFPGWHT
                     ECVVMSIKELGIPFDIHCGGIDHILIHHTNEIAQAEAAYGKVLARYWLHGEFLNFKEE
                     KMAKSKGNIVTIKKLIEKGVNPLSYRYLCLNAHYRSKLAFSWNALKAAQNSLDNLYNK
                     VREFQNQSSTNDKKNDGKKVKNYKKQFLSFINDDLNTPKTLALTWKLIKDKTISNKAK
                     YQLLLDFDKIFGLKLNRITKTTVPSKIKDLVQLREKYRKEKNWQEADRVRKEIEKLGY
                     QVEDTEKGPVIKENRM"
BASE COUNT          793 a          350 c          311 g          653 t
ORIGIN      
        1 cataagctcc cgcaaataaa attaatagat tctctttcct tttaaataaa ttctggagat
       61 tagtcctccc ttgtcttcct ctcttgctat tttaactact tttctgatca actcttcctc
      121 tggcgcctcc tccgacgccc attttaacat ctcttccact tcccatataa ttttttccat
      181 tttctcatcc tcccatcttt acgacgggaa tttaactgct aatttagtag taatcaaatt
      241 tccatcgtaa agatgctttc tttttctatt tttaaagaac tatttacctc cttttttacc
      301 tctttaaaat caacttttca gttataccac atctcattca tttttgcaag attatatagg
      361 ttttaaccct ttttttgttt taggcattcc cttgatccag ccttaaaacc agccatttaa
      421 taaaaaataa atagaaattt aaaataaaga aatatttgcc ttaaacctta aaaaagagta
      481 aaatagttta taatatgtta aaagtttata atactttatc tcgaaaaaaa gaaattttcc
      541 gacccttgca cgataaaaaa gtaggacttt acacttgcgg acctactgtt tattggtttg
      601 ctcatattgg caatctaaga acctatatat ttgaagacat tttaaaaaga actttggaat
      661 ataacggtta taaagtaaaa cacgtaatga atattactga tgttggccat ttaacttctg
      721 acgctgacac gggtgaagat aaattggaaa aaggagcaaa aagagagaaa agatcagtct
      781 gggaaatcgc cgaattttat acaagggttt taaaagaaga tttaaaaaat ttaaacatta
      841 taccccccaa tatttggata aaagccactg acacaattaa ggaacaaata gaattaatca
      901 aagtcttaga aaaaaaagga tttacttata caattgagga tggagtctac tttgatactt
      961 caaagcttaa aacctatggc cggctttggc caaaaaatgc tggtaaagaa ataaaacatc
     1021 gagtagaaat ggttcctggc aaaaaaaatc ctactgattt tgctctttgg aaattttcac
     1081 caaaaaatgt taaaagacaa atggaatggc aatcaccgtg gggtattggt tttcctggct
     1141 ggcacaccga atgtgttgta atgtcaatca aagaactggg cattcctttt gatatccatt
     1201 gcggtgggat agaccatatt ttaattcacc acaccaatga aatcgctcaa gccgaagctg
     1261 cttatggcaa agttttagct cgctattggc tccatggaga atttttaaac tttaaggaag
     1321 aaaaaatggc aaaatcaaaa ggaaatattg taaccattaa aaaattaata gaaaaagggg
     1381 taaatcctct ttcctatcgc tatctttgct taaatgctca ctatcgttca aaattagctt
     1441 tttcttggaa tgctttaaaa gcagcccaaa atagtttaga taatctttat aataaagtta
     1501 gggaatttca aaaccaaagc agtacaaatg ataaaaaaaa tgatggaaaa aaagttaaaa
     1561 actataaaaa acaatttctt agttttatta acgatgattt aaatacacca aagacccttg
     1621 cccttacttg gaagttaatt aaagataaaa caatttctaa taaagcaaaa tatcaacttt
     1681 tattagattt tgataaaatt ttcggtttaa aattaaacag aatcacaaaa acaacagtcc
     1741 caagcaaaat aaaagacctg gtgcagcttc gtgaaaaata ccgaaaagaa aagaactggc
     1801 aagaagccga cagagtaaga aaagaaattg aaaaattggg ctatcaggtt gaagacacag
     1861 agaaaggacc agtaataaaa gaaaatagaa tgtaaaatgt agaaagtttg aattttttga
     1921 aattctgtat tataaataaa tcaaatcaaa ttacaaaact acaagtaata aattttcaat
     1981 tttaaatgaa ttaattttaa aatttaggta gattcaagtt tttaacgcaa caataaaacg
     2041 ggatcgccac gtccttcggg ctctcaaaga aaatttcatt ttcttttcga cgagctcgct
     2101 tcgtcgc
//
LOCUS       DAOTQD010000056         2101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_41094_length_2101_cov_9.439394, whole genome shotgun sequence.
ACCESSION   DAOTQD010000056 DAOTQD010000000
VERSION     DAOTQD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41094_length_2101_cov_9.439394"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="P5570_02355"
     CDS             <1..626
                     /locus_tag="P5570_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRR39576.1"
                     /translation="EILKVPGEHNIQNALAALTCARVLKVPDKISFEALSKYQGAWRR
                     FEEHDLKIKNLNLKIISDYGHHPTEIKATLKAAREKFPGKRIILIFQPHQHQRTKFLF
                     KDFVKSFDETDILILTEIYGVAGREKGEKLSSKDLAMAIEKRWNKRGKRQEARGKKSV
                     VFIKDMEDIPDFLTKILKPNDVVMIMGAGNIYNLFLRLHQGEKNNLS"
     gene            complement(582..2039)
                     /gene="lysS"
                     /locus_tag="P5570_02360"
     CDS             complement(582..2039)
                     /gene="lysS"
                     /locus_tag="P5570_02360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRR39577.1"
                     /translation="MPTKKEIKKKREEKLDNFKKAGKNPYPQKVGSRQSIAGVIASFQ
                     KLKGKEVVLAGRIRAIREHGGSTFFHFEDGSGRFQAYLKKDQLGEDAYQFFLDNFDIG
                     DFVLVKGNLFETKRGEKTIEAKEYQMLSKALLPLPEKWHGLKDTEERFRKRYLDFLMN
                     PEVKEKFVLRARIISEVRKFLNKEGFIEVETPVLQTTYGGAAAEPFKTHLNALDLDLY
                     LRIAPELYLKRLLVGGFEKVYEMGRVFRNEGMDKSHNPEFTSLEFYWAFVDYEELMNL
                     VERMFDEIITNIFGSSKITYQGKEIDFTTPFERADFFELLTEKIGADPLKLKDDEIKK
                     IAQKLKISTEKKSRAKLLDDIFKKECREEIINPTFVLHQPIELTPLAKKDEKNPRLAA
                     RFQLVVAGWELTNAFSELNDPREQRKRFEEQAKEREEGIEETHPFDEDFVEALEYGMP
                     PAAGFGMGIDRLCALLTDSKTLREIILFPLMKPQK"
BASE COUNT          683 a          398 c          327 g          693 t
ORIGIN      
        1 aagaaatttt aaaagtaccg ggcgagcata atattcaaaa cgctttggca gctttaacct
       61 gcgctagggt tttgaaagtg ccagataaaa ttagttttga agcactgtca aaataccagg
      121 gtgcttggcg ccgttttgaa gaacatgatt taaaaattaa aaatttgaat ttaaaaatta
      181 ttagtgacta tggccatcat ccaaccgaaa taaaagccac cttaaaggcg gctcgagaaa
      241 aatttcctgg aaaaagaatt attttaatct ttcaacccca tcaacaccaa agaacaaaat
      301 ttttatttaa ggattttgtt aaaagttttg atgagacaga tattttaatt ttaactgaaa
      361 tttacggcgt ggctggcaga gaaaaagggg aaaaattaag ttctaaagat ttagcaatgg
      421 ctattgaaaa aagatggaac aaaagaggca agaggcaaga ggcaagaggc aaaaagagtg
      481 ttgtctttat aaaggatatg gaggatattc ctgacttttt gacaaaaatt ttaaagccaa
      541 atgatgtggt aatgataatg ggcgctggta atatttacaa tctatttttg cggcttcatc
      601 aaggggaaaa gaataatctc tcttagggtt tttgaatcag ttaaaagcgc acacaatcgg
      661 tcaattccca ttccaaatcc agcggctgga ggcataccat attccagtgc ctctacaaaa
      721 tcttcatcaa aaggatgagt ttcttctatc ccctcttccc gttcttttgc ctgctcttca
      781 aatctttttc tttgctccct tggatcattc agctcagaaa aagcattagt cagctcccac
      841 cctgccacaa ccaattgaaa acgcgcggct aatcgaggat tcttttcatc ttttttagca
      901 aggggcgtta attcaatggg ctggtgaagc acaaatgtag gattaatgat ttcttcccta
      961 cattcttttt taaaaatatc atcaagaagt tttgctcgag attttttttc tgttgagatt
     1021 tttaattttt gagcgatctt ttttatttca tcgtctttca attttaaagg atcagctccg
     1081 atcttttctg ttaaaagttc aaaaaaatct gcccgctcaa agggtgtagt aaaatcaatt
     1141 tcttttccct gataagtaat tttcgaagaa ccaaaaatat tagtaataat ctcatcaaac
     1201 attctttcaa ctaaattcat taattcttca taatcaacaa aagcccaata aaattccaat
     1261 gaagtaaatt cagggttatg agatttatcc attccctcat tccgaaacac cctccccatt
     1321 tcataaactt tttcaaatcc tcccaccaat aaccgtttta aataaagctc tggtgcaatt
     1381 cttaaatata aatcaaggtc caaggcattt aaatgagttt taaatggttc agctgctgct
     1441 cctccataag tggtttgtaa aactggtgtt tctacttcaa taaagccttc tttgtttaaa
     1501 aattttctta cctcagagat aatccttgcc cgcaaaacga atttctcttt tacttcaggg
     1561 ttcatcaaaa agtcaagata tctttttcta aaccgctctt ctgtgtcttt aagcccatgc
     1621 catttttcag gcaacggcaa aagagctttt gaaagcatct gatattcctt tgcttcaatt
     1681 gttttttctc ctctttttgt ctcaaataaa tttcccttta ccaaaacaaa atcaccaata
     1741 tcaaaattat ccaaaaagaa ctgataagcg tcttctccca attgatcttt tttaagatag
     1801 gcctggaatc ttcctgatcc gtcttcaaaa tgaaaaaatg ttgaaccgcc atgttcacga
     1861 atggctcgaa tacgacccgc taaaacaact tctttccctt ttaatttttg aaaagatgca
     1921 ataacccctg caattgactg acgagagcca accttttgag gataaggatt ttttcctgct
     1981 tttttaaaat tgtctaattt ttcctctctc ttttttttaa tttctttttt agtaggcata
     2041 ggtagaaatt ttattaaaaa tgtgatacaa cacaataaag taaaatgcaa aaatcaaaat
     2101 g
//
LOCUS       DAOTQD010000057         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_42024_length_2066_cov_7.063153, whole genome shotgun sequence.
ACCESSION   DAOTQD010000057 DAOTQD010000000
VERSION     DAOTQD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42024_length_2066_cov_7.063153"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(458..1831)
                     /gene="dnaA"
                     /locus_tag="P5570_02365"
     CDS             complement(458..1831)
                     /gene="dnaA"
                     /locus_tag="P5570_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRR39578.1"
                     /translation="MDLTSLWQSTLASLEQKISKASFVTWFSSTTIKKKEGDKIVLAV
                     PNIFVKEWLENKFEPELLSTIKEISPDVKEIKYIISNQKPKEYSSKKQSNQKLPVDFS
                     QPKIDFYQNRISGLNPKYTLKNFVVSSFNQLATACAISIIKKWSASSVSFKELSYNPL
                     FVYSGVGLGKTHLLEAIGNEVINARKKRKVKYIASPNFTSQVITAIRDQSIEKLVKEY
                     QTFDILIIDDIEFLAGKERTQEIFFRIFNELTEEGKQIILSSDRPPRAIEKIEERLRS
                     RFEGGMVTDISLPETEERLAILEQKMQEREVVIPREILEFIASNIKNNIRELEGALIK
                     SIVLFEREQSVEGTKNALQDIIKESKKKTSPEKIIEQVCNFYHIQKKEVLSKSRKKEF
                     VLPRQVIMYLMREEIGLSLPSIGAKIGNRDHTTVGYACEKTEEKLKKDGDFARDIEAI
                     KERIYNY"
BASE COUNT          608 a          346 c          329 g          783 t
ORIGIN      
        1 tgtttctttt gtttgccatt atgtaaacat aatattgttt ttgcataagg ggacgagatt
       61 gcttcgtttc gctcgcaatg acaaggaagg gagcttacct tcttttcgac acctctcttc
      121 gctcgtgtca atgacatcct tttttcttgt catcgcgagg agtccctcac cttagtaaaa
      181 ggtgagtgga cgacgtggcg atcccgttgc acgtccacaa acattccacg ggattgcttc
      241 gcttcgctcg caatgacaga ggggggggtg ccttcttttt aacacttcgt tgcggctcgt
      301 gttaataaca ataaataata aattattttt ttattgctat tgttattata gttgataata
      361 ggataattta caagtaataa tttattgtta tgggttaaaa taaaaaacaa aaaacttaaa
      421 acaaggggaa aaagtgttaa tcagtgagga taacttttta ataattgtat attctttctt
      481 ttattgcctc aatatctctt gcaaaatccc catctttttt taacttttcc tcagtttttt
      541 cacaggcata tcccacggtg gtatggtctc tatttccgat ttttgcacca atagaaggca
      601 gagaaagtcc tatttcttct ctcattaaat acattattac ttgacgcggc aaaacaaatt
      661 ccttttttct tgattttgac aaaacctctt ttttctgaat atgatagaag ttgcaaacct
      721 gttctataat tttttctggt gaagtttttt tctttgattc ttttataata tcctgaagcg
      781 cgttttttgt tccctcaacg ctttgttctc tttcaaaaag gacaatggat tttataaggg
      841 caccctccaa ttcgcggata ttatttttaa tgttactggc aataaattct aaaatttctc
      901 ttggaataac tacttctcgt tcttgcattt tttgttccaa aattgccaat ctttcttcgg
      961 tctctggtag agaaatatca gtcaccattc ctccctcaaa tcgagatctt aatcgttctt
     1021 cgatcttttc aattgccctt ggaggtcgat ctgaagaaag aataatttgt ttgccttcct
     1081 cagtaagctc attaaagatt ctaaaaaata tttcttgagt cctttctttg ccggcaagaa
     1141 attcaatatc atcgataatt aaaatatcaa atgtttgata ttcctttact agtttttcaa
     1201 tactttgatc gcggatagca gtaattactt ggctcgtaaa attgggtgaa gcaatatatt
     1261 ttacttttct tttttttctt gcatttatta cctcatttcc tattgcctct aaaagatggg
     1321 ttttccccaa tcctactccg ctatagacaa ataaaggatt atatgataat tctttgaaac
     1381 taacagaaga agcgctccat tttttaataa tggaaatagc acaggccgta gccaattgat
     1441 taaaactgct tactacaaaa tttttaagag tatatttggg attaagtccg gaaatgcggt
     1501 tttggtaaaa atcaatcttt ggttgagaga aatcaacggg cagtttttga tttgactgct
     1561 ttttagaaga atattctttt ggtttttgat ttgagataat atattttatt tcttttacat
     1621 caggcgatat ttcttttata gtggaaagca attctggttc aaatttattt tcaagccatt
     1681 cttttacaaa aatattagga acagcgagta caattttgtc cccttctttt tttttgatgg
     1741 tggtgcttga aaaccacgta acaaaacttg cttttgagat tttttgctct agcgaggcga
     1801 gggttgattg ccaaagggat gtaaggtcca tagaataatt tactaaagct tatacgaatt
     1861 aataaattta aattaacgca aatatttatt tattatatta tccccaaaaa attagtcaag
     1921 gaaaaaactg gggaaaagtt ggggaaaaca aaggttatat tagttaggtt caaaaattta
     1981 atgcaacaca tttactaatt tctaatttct aatttctaat ttctaatttt tattaaattt
     2041 ttaataaatc aattttcaaa cctttt
//
LOCUS       DAOTQD010000058         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_46241_length_1928_cov_7.850507, whole genome shotgun sequence.
ACCESSION   DAOTQD010000058 DAOTQD010000000
VERSION     DAOTQD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46241_length_1928_cov_7.850507"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="P5570_02370"
     CDS             complement(<1..1906)
                     /locus_tag="P5570_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRR39579.1"
                     /translation="MDIYTKPLLPNLSFKIRQGNSLISEINGVNINLKSEMLNIPRTV
                     RDKINRLIERKSAFFSGIRSANLKEIEEIEIIENRIFSDLLEEKIGKLENEIKRLSGD
                     LTLFKKGERPSEKGYIIQLELFKKEEDVERERIPEFIKKVEQRINFIETEIGKNKKVL
                     FSIKKKSQKDYFIWELDFIDVFSQKGGFDIVIGNPPYVRQELIAPPFEDKNSYSDDEW
                     RKIKKEYKEKLIQSVRNVWGNILKIDKKSDLYVYFYYQGLSLLRPNGIFCFINSNSWL
                     DVGYGAGLQEFLLKNMKPLYIFDNLVKRSFRQADVNTVIVLIQNQEINPDDDNIKFVA
                     FKKSFEDVSNAEIIKTIENTNQPVFDNENFRIYPKTKKELLLEGVEFPEEQENLTKID
                     PEHLPYIGNKWGGKYLRAPEIYFKILEKGKGKLVRLGDIAEVRFGIKTGANEFFYLRP
                     NGMTVKEVVEISEKNLDALIPVRNGAGWEGGIEARFLKPVIKSPRELKTIAVRLEDLN
                     YLVFMCHEPKSKLSGIKVLEYIKWGEKQKYQNRPSCRGRQRWWDLGEQLKNSYLWSRI
                     HNDSHRIYECNVYAADTFSVLYPKFDFYITGAILNATLSWFEKELYGRKVLGEGGLSS
                     LGVDLINIEII"
BASE COUNT          568 a          387 c          234 g          739 t
ORIGIN      
        1 ttataatttc aatatttatt aaatctacac ctaatgaact aagacctcct tccccaagta
       61 cttttctacc atacaattcc ttttcaaacc aagataaggt tgcattgagt attgcaccag
      121 taatataaaa atcaaatttt ggatataaaa cagaaaatgt atctgccgca tacacattgc
      181 actcgtaaat tctatgagaa tcgttatgaa ttcttgacca taaatatgaa tttttaagtt
      241 gttcccctaa atcccaccat ctttgtcttc ccctacaact tggtctattc tggtacttct
      301 gtttttctcc ccactttata tattccaaaa cctttattcc tgacaatttg ctttttggtt
      361 catggcacat aaaaacaagg tagttcaaat cctcaagtcg caccgcaatt gtttttaatt
      421 ctctcgggct ttttatcacc ggctttaaaa atcttgcttc aattccacct tcccaacctg
      481 caccgtttct aacaggtatc aaagcatcga gatttttctc tgatatttcc acaacttctt
      541 taacagtcat tccattaggt cttaaataga aaaattcatt ggctcctgtt ttaataccaa
      601 accgcacctc tgcaatatcc cccagcctta ccaatttccc ttttcctttt tccagaatct
      661 taaaataaat ctctggtgct ctcaaatatt tcccgcccca tttgttgccg atgtatggga
      721 gatgttcagg gtcaattttt gtcagatttt cctgttcttc tggaaattca acaccttcaa
      781 gtagaagctc ttttttggtt tttggatata ttctgaaatt ttcattgtca aaaacaggct
      841 gatttgtatt ttcaatcgtt ttgataattt ctgcattgga gacatcttca aaagatttct
      901 tgaaggcaac aaatttgata ttgtcatcat cgggattgat ttcttgattt tgtattaaga
      961 caatcactgt gtttacatct gcctgcctaa aagatctttt tacgagattg tcaaaaatat
     1021 aaagtggttt catatttttt aaaagaaact cttgcagtcc tgccccatat ccaacatcga
     1081 gccacgaatt ggaatttata aaacaaaaaa tcccatttgg acgaagtagc gataatccct
     1141 gatagtaaaa ataaacataa aggtcgctct tcttatcaat ttttagaata ttgccccaga
     1201 cattccttac cgattgaatc aatttttctt tgtattcttt tttgattttt ctccattcat
     1261 catcagaata actgttttta tcttcaaaag gtggagcaat gagttcctga cggacataag
     1321 gaggatttcc aatgacaata tcaaagccac ctttttgaga aaagacatca ataaaatcaa
     1381 gttcccatat aaaataatct ttctgtgatt ttttcttaat agaaaaaagt acctttttgt
     1441 ttttgcctat ttcagtttca ataaaattta ttcgctgctc cacttttttt ataaattcag
     1501 gtattctttc tctttcaaca tcttcttctt ttttaaatag ttccaactga attatgtatc
     1561 ctttttctga tggacgttcc ccttttttaa ataaagttaa atcaccagac aaccttttaa
     1621 tttcattctc taatttacct atcttctctt ctaataaatc actaaatatc ctattctcta
     1681 ttatttcaat ttcttcaatt tctttcaaat tagcacttct tattccagaa aaaaaggcag
     1741 attttctttc tatcagtcta tttattttgt ctcttactgt ccttggtata tttagcattt
     1801 cactttttag atttatatta acgccattga tttctgaaat gagcgaatta ccttgacgaa
     1861 ttttaaaaga aagatttggt aaaagtggct ttgtgtaaat atccatatac ttttcatttg
     1921 tctctata
//
LOCUS       DAOTQD010000059         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_46647_length_1916_cov_8.978506, whole genome shotgun sequence.
ACCESSION   DAOTQD010000059 DAOTQD010000000
VERSION     DAOTQD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46647_length_1916_cov_8.978506"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1035)
                     /locus_tag="P5570_02375"
     CDS             complement(79..1035)
                     /locus_tag="P5570_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRR39580.1"
                     /translation="MKNVKNALSVKNQRLFKVPNNSRINYWQVDHQIKNISKIEGVII
                     LPSSRAWEEYSWTRKYFFQKPKEGYFIWVKKEIDFPLFTCFSIASQKTRQNLENLLII
                     EKNLRVRLIGDCNSLKNDLNCTHKAKGKIILKEGVCLEYKHNHSWGKNDLVEPEYQFI
                     LEKNSKLNYTYKNLLTPQGLKIKTDFYLSEKASVIAKIILDAYSTKAMIIDNLYLKEK
                     ESRGEIQLKIVARKKSHIEAQSQIIAQSPGKGHLDCQGLLSDKTSIISLGPRLICKNK
                     DAELTHEASLGKISAEALNYLRQRGFSQKEAVDLIINGFLGK"
     gene            complement(1004..1708)
                     /locus_tag="P5570_02380"
     CDS             complement(1004..1708)
                     /locus_tag="P5570_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013413843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR39581.1"
                     /translation="MFLLEIKDLKVKADNKEILKGVNLKIKKGETQALLGPNGSGKSC
                     LCQVILGNPAYKITNGKIFFEGKDITKMPSEKRVKLGLALTWQSPPAIKGLKLSELCP
                     TIEKRSFLNIKEANDLLKRDVNVDFSGGEKKTSELLQVLCLNPKLVIFDEIDAGLDIK
                     KLGQVAEIIKTEFIRKNVSVLLITHSGQILNYLKPDITNVMLDGKILCQKKDFKNVLR
                     IIKKYGYEKCKKCPVR"
     gene            complement(1790..1880)
                     /locus_tag="P5570_02385"
     tRNA            complement(1790..1880)
                     /locus_tag="P5570_02385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Ser,seq:gct)
BASE COUNT          580 a          348 c          269 g          719 t
ORIGIN      
        1 ttggaatttt tacgctcgtg tcaaatacaa aaaaatcaaa attatctatg gcggttcttt
       61 aactgcgatc agaggctatt attttcctaa aaagccattg ataattaaat ctactgcttc
      121 tttttgggaa aatccccgct gtcttaaata attaagggct tcagcagaaa tcttccccag
      181 tgacgcttca tgagtaagct ctgcgtcctt gtttttacaa atcaaacgtg gacctaaaga
      241 aattattgat gtcttgtcgc ttaacagtcc ttgacaatca agatgtccct tgccagggga
      301 ctgggcgatg atttgagatt gagcttcaat atgacttttt tttcttgcca caatctttaa
      361 ttgaatttct cccctgcttt ctttttcttt taaataaaga ttgtctataa tcattgcctt
      421 ggttgaatag gcatctaata taatctttgc tataactgat gctttttctg aaagataaaa
      481 atctgttttt atctttagtc cctgtggcgt taaaaggttt ttataagtgt aatttaattt
      541 tgaatttttt tctaaaataa attgatattc tggttctacc aaatcatttt taccccagga
      601 atgattatgt ttatattcca aacaaacacc ttcttttaaa ataatttttc cctttgcttt
      661 atgagtgcaa tttaaatcat tttttaatga attgcaatct cctattaaac gcactctcag
      721 gtttttttca ataattaaca aattttcaag attctgacgg gttttttgag aagcaataga
      781 aaaacaagta aacaaaggaa aatcaatctc ttttttcacc caaataaaat aaccctcttt
      841 tggtttttga aaaaaatatt ttcttgtcca ggaatattct tcccaagctc ttgaacttgg
      901 caaaataatt accccctcta tttttgaaat attttttatt tgatgatcaa cctgccagta
      961 atttattcta ctattattag gaactttaaa caatctttga tttttaacgg acagggcatt
     1021 ttttacattt ttcatagccg tattttttaa ttattctaag aacattttta aagtcttttt
     1081 tctggcaaag aattttccca tcaagcatta cattagtaat gtctggcttt aaataattca
     1141 aaatttgtcc tgaatgagtg ataagcagca cagagacatt ttttctaata aattccgttt
     1201 ttataatttc tgctacttgc cccaattttt taatatcaag accagcatca atttcatcaa
     1261 aaatcaccag tttcggatta aggcacaaaa cctgtaataa ttctgaggtc tttttttctc
     1321 cacctgaaaa atctacattt acatctcttt ttaaaagatc atttgcttcc tttatattta
     1381 aaaaagatct tttttctatt gtggggcaca gttctgataa ttttaatcct ttaatggctg
     1441 ggggagattg ccaagtaagc gctaatccca actttacccg tttttcagaa ggcatttttg
     1501 taatatcttt accttcaaaa aatattttac catttgttat tttataggca ggattgccca
     1561 aaatcacttg gcaaagacaa ctcttgccac tgccattggg tcccaaaagg gcctgtgtct
     1621 cgcctttttt aattttaaga tttactcctt ttaggatttc tttattatct gccttgactt
     1681 ttaaatcttt tatttccaaa agaaacataa aaaatcaata aatagattaa gatcttaata
     1741 aacaaaaccg tatttatcct gctttgtaga atttcagtga aaaaaataat ggcggtgggt
     1801 gtaggattcg aacctacgag ggacggttta ggtcccacgg gttagcaacc cgttgcttta
     1861 agcccctcag ccaacccacc aacctactgc tttgcagtag aattttcaat tttcaa
//
LOCUS       DAOTQD010000060         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_47658_length_1885_cov_8.874863, whole genome shotgun sequence.
ACCESSION   DAOTQD010000060 DAOTQD010000000
VERSION     DAOTQD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47658_length_1885_cov_8.874863"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1510
                     /locus_tag="P5570_02390"
     CDS             197..1510
                     /locus_tag="P5570_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HRR39582.1"
                     /translation="MKFELSFTNKEITPWGGMVFLKQMLDKIGFREQIEKCESLPVSL
                     SNNSYKKEVLLESFITSIWCGANRFLHTEITRADKALGEIFDWRKTPAQDAYKRYFGK
                     FTQHINQQVGHHFFSWFFQNLNLNYFTLDIDSSVITRYGEQEGAKKGYNPKKKGRNSH
                     HPIIAFVNDVKMVANFWLRSGNTSSANNFVGFLEETLLNFGDKKVGLVRLDSGFFQKD
                     IMDYLELKTLQYIIAAKFTHPIQHLIDQQDFWIKVDEGIEICDKYYQAKNWEKPRRIV
                     IVRQKIAQRPNAAGRILSLFPEDEIHRNYRYSAYITNQEQSATDVWRTYRNRGDAENR
                     IKELKADFGAESFNLKGFFPTEAALIFSMIAYNLMSIFRLFVLQEKTQKTLSTLRYRT
                     FAIGAYFEKVGDTLKLKIALTKKRRKWFVGIWDYPIDLSQKISTA"
     gene            1555..>1885
                     /locus_tag="P5570_02395"
     CDS             1555..>1885
                     /locus_tag="P5570_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HRR39583.1"
                     /translation="MSVFKDHKISLKQVLEFIPEALLTHLSATSKVDHYSKVLHGKKM
                     FYLLLFCIFDNEKLSQRTLEDTFNSSGFKALFGLGEEEKVRRSSISERLSKIDPNYFK
                     EIYEQMYG"
BASE COUNT          656 a          325 c          354 g          550 t
ORIGIN      
        1 tgtttaagag aaatcttgtg gtctttaaat actgacataa gttattgatt atcagctata
       61 aagatacgaa attaaattca aataaacaag ttaaataatt gataatcaac ctgttaaacc
      121 caaattccgc aatagaaaaa aaagagtatt ttagtattct aatatccaac tgcgtagcag
      181 tagaaaaaaa taagcgatga aattcgagct atcctttacc aataaagaga ttacgccttg
      241 gggaggcatg gtgtttttaa agcaaatgtt ggacaaaatc ggctttagag agcaaattga
      301 aaaatgcgaa tctttacctg tgtcactttc caataattct tataaaaaag aagttttgct
      361 tgaatctttt attacgagta tttggtgtgg cgccaatcgt tttttgcaca cagaaattac
      421 ccgtgcagat aaggctcttg gggaaatatt tgactggcga aagacccctg ctcaggacgc
      481 atataaacgc tattttggca agtttacaca acacatcaac cagcaagttg ggcatcattt
      541 tttcagttgg ttttttcaga atttgaacct caattatttt acgttagaca ttgattcatc
      601 tgtaattact cgatacggag agcaagaggg agcaaaaaaa ggctacaatc ctaagaaaaa
      661 aggaagaaac agccatcatc ctatcattgc atttgtgaac gatgtaaaga tggtcgctaa
      721 tttttggctc aggagcggca atacttcttc ggcaaataat tttgtaggat ttttagaaga
      781 aacactctta aattttgggg ataagaaagt aggattagtt cgtttggata gtggtttttt
      841 ccagaaagac attatggatt atcttgaatt aaaaacactc caatacatca tcgctgcaaa
      901 atttactcac cccattcaac acttgataga ccagcaagat ttttggataa aagttgatga
      961 gggcatcgaa atttgcgaca aatattatca agcaaaaaac tgggaaaagc caagaaggat
     1021 agtcattgta aggcaaaaaa tagcacaacg gccgaatgcg gcaggccgaa tattaagcct
     1081 gtttccggaa gatgaaatcc atagaaatta tcgctattca gcctatatta ccaaccaaga
     1141 acaatcggca acagatgttt ggagaacgta ccgaaacaga ggcgatgcag agaatcgaat
     1201 caaggaatta aaggcagatt ttggagccga aagttttaac cttaaaggct ttttccctac
     1261 agaagctgca cttatatttt cgatgattgc ttataatctg atgtcaattt tcagactgtt
     1321 tgttcttcag gaaaaaacgc agaaaacatt atctacacta cgatatagaa cctttgctat
     1381 tggagcttat tttgaaaaag taggtgacac actcaaactg aagattgcac tcaccaaaaa
     1441 acgcagaaaa tggttcgtcg gaatttggga ttaccccata gacttatctc aaaaaatttc
     1501 aactgcgtga tttgggttta atttcgtatc tttatagctg ataatcaatt acatatgtca
     1561 gtttttaaag accacaaaat ctccctcaag caagttttgg agtttattcc cgaagcgctt
     1621 ttaacccatc tttctgcaac ttctaaagta gaccactata gcaaagtgct tcacggtaag
     1681 aaaatgtttt atttgctttt attttgtatt tttgataatg aaaaattaag ccaaagaacc
     1741 ttagaagaca cctttaacag cagtggtttc aaagcacttt tcggtttggg tgaagaggaa
     1801 aaagtacgca gaagctccat ctcggagagg ctctccaaaa ttgaccctaa ttatttcaaa
     1861 gaaatctacg agcagatgta cggaa
//
LOCUS       DAOTQD010000061         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_51583_length_1784_cov_22.078080, whole genome shotgun
            sequence.
ACCESSION   DAOTQD010000061 DAOTQD010000000
VERSION     DAOTQD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51583_length_1784_cov_22.078080"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1616)
                     /locus_tag="P5570_02400"
     CDS             complement(18..1616)
                     /locus_tag="P5570_02400"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HRR39584.1"
                     /translation="MVVFGGKMFIKRTIGGTKEKPIAYLQLAQSYRDENGKPRHRILC
                     TLGKEKEVIGIAEVLAKRFAKLSSKLLVLDKETDFLGRSFLFGPVLAAEAIWRQLNLD
                     EMLKDVEKEYKVEYDLSKCVKLMTINRLCEPKSKLSIMEWKEDLYCEGMKGIELQHLY
                     RSLDILAENKDFILKGLYRKSVELFKAEVKVVFYDLTTLYSESVRESLLKRFGYSKKN
                     KSDCVQVVMGLVISRDNIPLYYELFPGNTYEGSTVKGIVEAMKRKFEIEKVIFVGDKG
                     LLGKGVLAEIEKLGLEYIVSAKISQSAKKDKKEIFTQEGMREIGEGVKIKEIKINGRR
                     MIVGYSEERKKRDRAIREELIEKIKEKIKKDKKGIIAKPYYRTYLDIKAEKIEINEEK
                     IKLKEEEDGYFGFWTNNKELKEEEILKAYKMLWQIEASFRCLKSSLDLEPLYHYTPKR
                     IEGHTLMCFISFYILNAMTRKIEDLSYEKIIESLKRIRVVEIKTDDQKIFARTEISGL
                     NNQILRSLNIKIPPTILKEENVVE"
BASE COUNT          458 a          432 c          203 g          691 t
ORIGIN      
        1 ggggacactt atactatcta ctccactaca ttttcttctt ttaatattgt tgggggtatt
       61 ttgatgttca ggcttcttaa gatttggttg ttcagtccgc ttatttctgt tcttgcaaaa
      121 atcttttgat catcggtttt gatttctacc actcttattc tttttaaact ttctataatc
      181 ttttcgtaag ataaatcttc tatttttctt gtcatcgcat taagtatgta aaaacttata
      241 aagcacatca atgtgtgtcc ttcaatcctc tttggtgtgt aatggtataa aggctctaaa
      301 tcaagcgagc tttttaaaca cctgaaagac gcttctatct gccatagcat cttgtatgct
      361 ttcagaattt cttcttcttt taattctttg ttgttcgtcc agaatccaaa atatccatcc
      421 tcttcctctt ttaactttat tttttcttcg tttatctcta tcttttcagc ttttatgtca
      481 agatatgttc ggtagtaagg cttggctatt atcccttttt tgtctttctt gattttttct
      541 tttatctttt ctatcaattc ttctctaatc gctctgtctc gcttcttcct ctcctcgctg
      601 tatccaacta tcatccttct tccgtttatc tttatttctt tgattttgac gccttctcct
      661 atttctctca tcccttcttg tgtaaatatc tctttcttgt ctttcttcgc actctggctt
      721 atcttcgctg atactatgta ttcaagccct aatttctcta tttctgccaa aactcctttc
      781 cccaacaatc ctttgtctcc cacaaaaatt actttctcta tttcaaattt ccgcttcatc
      841 gcttcaacta ttccttttac tgtgctgcct tcgtatgtgt tgcccggaaa aagctcataa
      901 tacaaaggaa tgttgtctct gcttataaca agccccatca ctacctgaac gcaatcgctt
      961 ttgttcttct tgctgtatcc aaacctcttt agcaaacttt ctcttacact ctcgctgtaa
     1021 agcgtggtca aatcataaaa aaccaccttc acttccgctt tgaaaagttc aacgctcttt
     1081 ctatacagcc ctttcagtat aaaatcttta ttctcagcaa gaatatccaa actcctgtaa
     1141 agatgctgta attcaatccc tttcattcct tcacaatata agtcttcctt ccattccatt
     1201 attgataact tgctcttagg ctcacatagc ctgttgattg tcatcaactt gacgcattta
     1261 gataaatcgt attcaacctt gtattccttc tcaacatcct tcaacatttc atcaaggtta
     1321 agttgcctcc atatcgcttc tgccgcaaga acaggaccaa acaaaaaact tcttcccaaa
     1381 aaatctgtct ccttatcaag aacaagaagt tttgaagaaa gtttcgcaaa tctttttgcc
     1441 aaaacttccg ctatccctat tacttctttt tctttgccta aagtgcataa aattctgtgt
     1501 cttggcttcc cgttttcatc tctgtaactc tgagcaagtt gcaaatatgc aattggtttt
     1561 tcttttgttc ctccgattgt tctttttatg aacatctttc ctccaaagac taccataata
     1621 acatattcaa accgctctgt caatagttaa tatctaccct ataccacaaa aaatgccact
     1681 acaaaatcgg caaaaataaa aaaccaaaaa tcataactaa ctcattctca aagacttata
     1741 tctttaccaa aaccaaaaat taaccctcat ctgtcaaagt cagg
//
LOCUS       DAOTQD010000062         1767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_52283_length_1767_cov_4.797313, whole genome shotgun sequence.
ACCESSION   DAOTQD010000062 DAOTQD010000000
VERSION     DAOTQD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52283_length_1767_cov_4.797313"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="P5570_02405"
     CDS             complement(<1..205)
                     /locus_tag="P5570_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRR39585.1"
                     /translation="MEKETKNQNQKNQEREISEELQESYLDYAMSVIVSRALPDARDG
                     LKPVQRRILWSMWDSGILHSAKTR"
     gene            complement(316..564)
                     /locus_tag="P5570_02410"
     CDS             complement(316..564)
                     /locus_tag="P5570_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39586.1"
                     /translation="MKDFFKKLQNADISVRKRYLIIFSVLAMVVVISLWIFYLKTVVN
                     RPPESQGALKNFFLNLGQWFESTFFKIKEGNVINIERQ"
     gene            complement(568..1191)
                     /locus_tag="P5570_02415"
     CDS             complement(568..1191)
                     /locus_tag="P5570_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR39587.1"
                     /translation="MIITFYGEGCFKVQSGEMVVLTDPPAKESGLEAPRFKFDIMLRT
                     LSAFPPEAKNFYELVGPGEYNFKIADKEIDILGIFLAKESTDKFIKTIYLVNLEDIKL
                     CFLGHLSNDLSPEIIEKLEEIDILFIPAGGKPFIDQKRAASLIKQLEPKVVVPSFYKI
                     SNLKRPADDLEKFLKECDYEASQEEKLVIKKKDLQTIKGTKIISLKV"
     gene            complement(1486..1659)
                     /locus_tag="P5570_02420"
     CDS             complement(1486..1659)
                     /locus_tag="P5570_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39588.1"
                     /translation="MRVKPVVDRAQQRLVAFFEGNLLKNEEKDWKKNLVLDKSVVKSK
                     ELDSLLVKPFLLF"
     gene            1664..>1767
                     /locus_tag="P5570_02425"
     CDS             1664..>1767
                     /locus_tag="P5570_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR39589.1"
                     /translation="MLIVIAIIAILAAIIILAINPIEQMKKTRDARRMA"
BASE COUNT          600 a          295 c          216 g          656 t
ORIGIN      
        1 ttctagtttt tgcagaatga agaattccgc tatcccacat tgaccaaaga attcttcgct
       61 gaacaggctt taatccatca cgagcatcag gtaaagcgcg cgaaacaatt acgctcattg
      121 cataatctaa atatgattcc tgtaattctt cagaaatttc cctttcttga tttttttgat
      181 tttgattttt tgtttctttt tccatttata tctatgtcct tacaaattta cgaatattca
      241 ctaatataca aatgcggctt taaaaaagat tttttacgga tttgcatatt tgtattaatt
      301 tagatattgg taagatcatt gcctttcaat attaatcaca ttaccttctt taattttaaa
      361 gaaagtagat tcaaaccact gaccaagatt caaaaaaaaa ttttttaaag caccttgact
      421 ttctggtggt ctgttaacaa cggttttcaa ataaaaaatc cataaactaa taacaacaac
      481 catagcaagc actgaaaaaa taattaaata tctttttcta acgcttatat cagcgttttg
      541 tagttttttg aaaaaatctt tcattcttta aactttaagg gaaataattt ttgtcccttt
      601 aatcgtttgc agatcttttt tcttgatcac taatttttct tcttggcttg cttcataatc
      661 gcattctttc aagaattttt ccaaatcatc agctggcctt ttcaaattac tgattttata
      721 aaaactggga acgacaactt ttggctcaag ctgttttatc aaacttgcag cccttttttg
      781 atcaataaat ggtttaccac cagcaggaat aaaaagaata tctatttctt ctagtttttc
      841 aataatttct ggagataaat cattagaaag atggccaaga aaacaaagtt taatatcttc
      901 taaattaaca aggtaaatgg ttttaataaa tttatcagtt gattctttgg ctaaaaaaat
      961 tcccaaaata tctatttctt tgtcagcaat tttaaaatta tattcaccag ggccaactaa
     1021 ttcataaaaa tttttagctt ctggcggaaa agcacttaaa gttcgtaaca ttatatcaaa
     1081 tttaaaacgc ggcgcctcta aaccgctttc ttttgccggc ggatcagtta aaacaaccat
     1141 ctcgccagac tgaaccttaa aacatccctc gccataaaaa gtaataatca tgataaaaat
     1201 tcaaaatgca aaattataaa tttaaatttt ctgctttaac tttcaggttt tgactttcga
     1261 ttttaattaa gtttatttta ccatgcttaa acttaatttt caaattaaat tttaaatttt
     1321 gaatttgatt tgacatttga tattgagatt ttggttgtat aatatatgtt gtataatgtt
     1381 ataatacata ttgcatgata cataatatat gtagtaaact taaaacatta tgtttaatct
     1441 atattttctc aatctaagag aaagaataag ctcttctcaa tcttctcaaa acaacaaaaa
     1501 aggttttacc agtagtgaat caagttcttt gctttttaca acacttttat ctaaaacaag
     1561 gtttttcttc caatcttttt cttcattttt taaaaggttt ccttcaaaaa aagcaactaa
     1621 gcgttgctgg gctcgatcta ctaccggttt tactctcata gaaatgttaa tagtcatagc
     1681 tattattgct attctagcag ccattattat tcttgctatc aatccaattg aacaaatgaa
     1741 aaaaaccaga gatgcaagaa gaatggc
//
LOCUS       DAOTQD010000063         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_2826
            NODE_54778_length_1710_cov_5.946828, whole genome shotgun sequence.
ACCESSION   DAOTQD010000063 DAOTQD010000000
VERSION     DAOTQD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867850
            Sequence Read Archive: SRR5007134
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 487
            CDSs (total)                      :: 452
            Genes (coding)                    :: 447
            CDSs (with protein)               :: 447
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54778_length_1710_cov_5.946828"
                     /isolate="AMR_MDS_2826"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..214
                     /locus_tag="P5570_02430"
     tRNA            139..214
                     /locus_tag="P5570_02430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:172..174,aa:Ile,seq:tat)
     gene            249..1466
                     /locus_tag="P5570_02435"
     CDS             249..1466
                     /locus_tag="P5570_02435"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRR39590.1"
                     /translation="MEGEKMFYLEKKEEKKNLLFEGPELISPEEEREQKDEMSFSFSS
                     DTRRYSLGHKNGKPFFVVEYFGSLPDELSETEKAKFTKEIRKQGDAEQVVYVLKHGEV
                     PEQIEKMKESNLSQDKKYRVAPSRDFKKDIMNKFFANETNKQVMNEILARHSKLNSKE
                     IAKIVDQAFDAFRKNDSEVIRILSNHFPKEIAEKVASEIYAKNLPKPQEIEVDKLAEI
                     LKNKKVLFYTGAGISMASGVPNMDQLQESLGIAMSEKTDDFLKKSIIDPQQIIDLWKE
                     FMKASFEKPATPAHQALSSLSQKLKSKIFTENVDHLQEKTGVKAIHLTGPWLKENIQP
                     EWLKDIDIVLTVGLSSDDRGFLGWYKENNPKGKIIAINLNQPPYLGNEDLILKGDCQK
                     LVPDLEKTFSAKI"
BASE COUNT          615 a          242 c          339 g          514 t
ORIGIN      
        1 aagagatgat tctttggtcg gcatgtagct cagtggttag agcatcgctc ttatatcaag
       61 tatttccttt attaatgttt tttaatagaa atccttgata tattgattat taccttttaa
      121 aagagatgat tatttggtcg gcatgtagct cagtggttag agcatcgctc ttataaagcg
      181 agggtcgcag gttcgattcc tgccatgccg accaaagaat cattaaattt aaagattttg
      241 ataactttat ggaaggggag aaaatgtttt atctggagaa aaaagaagaa aaaaagaatc
      301 ttttatttga aggtccggaa ttaatttcgc cggaagaaga gagggaacaa aaagatgaaa
      361 tgagtttttc tttttcttct gatacaaggc gatattccct tggtcataaa aatggtaagc
      421 catttttcgt agttgagtat tttggttcat tgcccgatga attaagtgaa acagaaaaag
      481 caaaatttac aaaagaaata agaaaacagg gtgatgctga gcaagtggtt tatgttttaa
      541 aacacggaga agtgccagag caaattgaaa aaatgaaaga atctaacctt agtcaagata
      601 aaaaatatcg cgttgctcca tcaagagatt ttaaaaaaga tataatgaat aaattttttg
      661 ctaatgaaac caataagcag gtaatgaatg aaattttagc acgacattca aaattaaact
      721 ctaaagaaat tgcgaaaatt gttgatcagg cttttgacgc ctttcgtaag aatgactcgg
      781 aagtaattcg cattctttca aatcactttc caaaagaaat tgcggaaaaa gttgcctctg
      841 aaatttacgc aaagaattta ccaaaaccgc aggaaataga ggttgataaa ttagcggaaa
      901 ttttgaaaaa taaaaaagta ttattttata ccggagctgg tatttcaatg gctagtggtg
      961 tccctaatat ggatcaatta caagagtctt tggggattgc aatgtcggaa aaaactgacg
     1021 attttcttaa aaagtctatc attgatccac aacaaataat tgacttatgg aaagaattta
     1081 tgaaggccag ctttgaaaaa ccagcaactc ctgctcatca ggcacttagc tctctttctc
     1141 aaaaactaaa atcaaaaatc tttactgaaa atgttgatca tttacaagaa aaaacggggg
     1201 taaaagcgat tcatttaact ggtccttggt taaaagaaaa tattcagcca gaatggttaa
     1261 aagatattga cattgtgctt acggttggat taagttctga tgacagaggt tttctcggtt
     1321 ggtataaaga aaataatccg aaagggaaaa ttattgcaat taatttaaat cagccaccct
     1381 atcttggtaa cgaagatttg attttgaaag gagattgtca aaaacttgtc cccgatttgg
     1441 aaaaaacatt ttccgcaaaa atataaaatt agtaaactga atatatctgt gatacattag
     1501 attaaatagt tttgcggatg atgaaaagtg aagatgagta aaatcctttt tctgtttagg
     1561 cggttagctc agttgggcta aaagccaaag tcttggcttt tagcgggcaa ttaccttgtt
     1621 aatattcgaa aattaattgc ccggggaaga gcattttccg tcgaagacgg aacacgcgat
     1681 taaaaccagt ttactcgatt tataggtagg
//
