LOCUS       DAOTQL010000001        35524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_1310_length_35524_cov_5.304830, whole genome shotgun sequence.
ACCESSION   DAOTQL010000001 DAOTQL010000000
VERSION     DAOTQL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35524
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_35524_cov_5.304830"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..628)
                     /locus_tag="P5293_00005"
     CDS             complement(53..628)
                     /locus_tag="P5293_00005"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HRR48349.1"
                     /translation="MKFCFVTNIYYFCYKIEMMSIEEKILQRIEAFPKGELFLPADFS
                     ELGSSEAVRLSLFRLEKEGVIMRVAQGIYVRPKESNIIGKLIPSAEEIAEAIAKRDRI
                     RTVPTGSYALNALGLSTQVPMNIVLLTDGSAREIKVGKRKIKFKKTTPKNLLAKGKIS
                     RLVIQALKEIGNGKGKTRQGEKGKGSQRKKI"
     gene            complement(644..4318)
                     /gene="pglX"
                     /locus_tag="P5293_00010"
     CDS             complement(644..4318)
                     /gene="pglX"
                     /locus_tag="P5293_00010"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HRR48350.1"
                     /translation="MKLSEHTEQIRQLIDRAFRNRLGRMGISENLLSDIETIPSEYRA
                     ERIRMETIREVFIAETGSISDAYEKLVEELTFTLFNRLAALKVMEAHTLHPEIVTRRE
                     SHGGRSFAHLAWLEQNPQARSEEAEGLLPFLEEQLQKLAVDIPLFSPQHPYHLLPTAL
                     ELLSIINAFNQVETDAQVEADIWRSDDILGWLYESYNNSKKAAHKASGEKTEYNKVSI
                     QSQVYTPRWVVQFLVDNSLGKLYLEMYPDSEIGHKYKIANAPTTQTRERKPLHEIKMI
                     DPATGSGNFLLYGFDLFYDLYTDQIENYGADYNEADIPKLIIENNLYGVDLDDRAIQL
                     AQLGLYIKAKRKKRSVKIEHFNIVSSDFFLPDYEEVQQIFERGNTLDSKQKEIIRDIW
                     NDLQQAYKFGSLIRLEEKLSIHLHGIKQDGLTLFGAQEVENYETFKAQFFTNLQKAVA
                     QNTARQGQTFLNNKTQDAITFLQLLTQKYDVAVANPPYTDSADFGPELKKFVEANYKQ
                     PYKFNTNLYAAFIKRCCEITTDDGYVGMIHPHTFMFIKTFEDVRKYLIENTHIDIMVD
                     YGLDRVNLFGPGILLDATWYVLSKKKKETPGIYFNITANQQEKAKQASLKQAYEDAIN
                     NRPNPRLYSLPQEKLKIIEGWPFIYWISDGFREKFKGITMKAIFEINEGLKTSNNIKY
                     FRSPLEINYEFNSKYSKIYKGGPYNKWFGNIWLTIDFQNQRRNIENESSPGFSGESNY
                     LREGIVCPSVGTKGPSFRFKPSDSIFSNADLGIFFKNKAEKNYYYILAILNSSLIGYV
                     NEMLNPTVNITVGDIQRIPFIKPSQILEENISAISLENVSIKKHLCSYRITETYYTQS
                     PLLAFQGATLKDRVLAYLNYENDQLTQVLINEAIIDELIFEVYELSDADREQVEAKMG
                     KSIGSLPVSSEAKDAFLSQLENPLTEVMDHIQNLSVTTFDEQQVREIKEGFETLYQSN
                     NDLEEFCIRHQVNPINVWYWFKETNTVPQARAAEIALEFLADSIRTLLQQDEDGIIPL
                     VGLPGEEALSQRLEKHCLQNGFTSAQYMQLDSLLGRPINEYLEHHFFNQLSNHLNLFM
                     YLPKTPFIWHLSSGRNQGFEAYILIYKWNRDSLFKLKSRYISQRVQNLEYRQITLQDV
                     NTAQAQAEKEKIRLQLQEIEVFKTKIEELIAEGYDPKLDDGVGKNIAPLQKKGLLRAE
                     VLNTAGGKKSQLERYLNADW"
     gene            complement(4321..6015)
                     /locus_tag="P5293_00015"
     CDS             complement(4321..6015)
                     /locus_tag="P5293_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRR48351.1"
                     /translation="MEINDLQYLINQGEGLRIEFKDAKDSVPSSFYETVVSFSNTDGG
                     TILLGVDDDGKVKGIDGNAEVSLMKDIITALNSRDCIDPPVYVQPFCINHPDGKVMVI
                     QIPASSQIHNHAGTIYSRDFESDLDITNNQQRLSDLYLRKRNFFTEAIIYPHLSMTDL
                     DDKLFDKAFTLIRNNKSDHPWLMANKEQLLREATLWRKDFQTGQDGLTLAAALIFGKD
                     ETIQSLLPAYKVEGMLRKENKDRYDDRINPPLRTNLIDTYLELKAFINKHLPEKFFME
                     GDQRVDLRDKIFREVIGNAIVHREYTSALSTELIITASEVVITNPNKPYFHGVIDPFG
                     FNPYPKNPNIRKFFTAFGWTDEIGSGVRNTNKYLPLYAPGAHPLFVENDIFKTVIPLR
                     FVSLGNYADSFISWLGISESYASHLSEGLQQIELPAECFEQTWQQVLLHLVPCWHQKG
                     TQLNALDWFENQVFAEDEIKKVPGWHEKSTQLLKKKNRYLIGILSLCSLPIALSDLMA
                     AFDYKNAKTFRDNYLKPLRDSGLVVFTIPEKPTDPDNKYTLTQQGKAFLGGLLNKF"
     gene            complement(6019..9615)
                     /gene="brxC"
                     /locus_tag="P5293_00020"
     CDS             complement(6019..9615)
                     /gene="brxC"
                     /locus_tag="P5293_00020"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HRR48352.1"
                     /translation="MKIKEILTIDLTEDIKNVIDLEDISEKEIQAEIESYIVTDGLAR
                     EYVGFVDTFTSDILETGVWISGFYGSGKSYFGKLLGYMLSNPNIGGTPARDRIIQRFT
                     GINDEALVKNALSRLNTITSRVVFLDIAKQDTSKGIAYTLFGNFLRSLNLPENEHGIF
                     LFQLMLSERKTIVQDFIFDVSGLDWNEIRTSSFKYAKAIKELYINLGNTESDYDNLMT
                     TIRRDMDQFSTSRLREELSNYLQVEKNEKVIFLFDEASEAINQKKISLLDLEGLSESL
                     SSLGGKVWTIAIAQEKLDDVINNSNVSKAQLTKVTDRFKTKIHLEATEVDVIIRNRLL
                     KKNESGIQKLTDHFLKNSGKIADHAAIHGAGISKTDSADNYATYYPFYKYQFDLLQNF
                     LFGTKGYASTKVAARGMIITTYDILKQEVQHTDLFHTVTGWHIAKEGQPQPPVRLVSR
                     YDNAERILLESGSTISGRRLLETINFLTGADVTPSSLPNIIKCFISDPDEQFKVQEEI
                     VKALDLLTEAKVLLDTNKTYRITSDIEQRLLDEMNGFTVQGFVKKKQLVTAYKSANFT
                     KTIGKVSDNGLQFDFYITTDNDDELTSPNLKYLRVKIKSIYNISEDRSADIDNLKVQH
                     QNDKDLLWLVPDNSYFKELDRLIEEVERITYLEQKYNNPNSEEGKILISFSTSKGEKQ
                     NRIKELVEESLFNSTAIYLYNTFQLDNDNWQTTLQAQQRSIIQNVYSKRLASQLSDTV
                     AAAVIKEANSGRLHQYFTGSDFAFFDTQGNFIGENLKVSEEILYKVRNTFVDGATLEN
                     DLEHPPTGFTFGTVISSVAALMRAGKLIAKYNGAEKFSWRDEGVTTIFNVAREFRKAS
                     FKAVSKSLSALQKQELAQFLLEVEVEKYIGRKIDYNTNDFELVSAVRDMARHFADKVT
                     TLRNSEKEFDKLFPRAEENAAYLGGFTGAVSEANYIDKAKEFLASKDAFYKALIEIEK
                     VEKFIRNNLPKVKVWKAFVIAVQDELNKAAKIDATIQKLKEDFDIYFQGDVIKNFVSL
                     QESAQKTKDRYHQLFSEAMKECAAKYTEIDTLASDLIKEIEALPSGLNDATLSKATAL
                     HQYAGQRKIATVNIDFDVKDKHSRFTYSEVLSFIELYQSKKAEIEIARAGLVKTKAPD
                     SQPGTFQLPTVKKYAVKMPATKMKVSEYKIWLHGELQKLAGASDNDEIEINA"
     gene            complement(9630..10190)
                     /locus_tag="P5293_00025"
     CDS             complement(9630..10190)
                     /locus_tag="P5293_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48353.1"
                     /translation="MSRSLNPEYTISQIKRIVESNKRSEIRLNANGGNSILLVCPPEQ
                     EFEFIEKLTELMPQETYHIIDINEILISFVETYKSDLAEMFDLLKGSVHQIFKAPEGE
                     TGPDLFKHLLSAIKQTYSEQKTPILIHSGALYGSGIDNIHLMENELVMKSKLPLIILY
                     PATQEGDRLMFLNTRPASKYRCMIVK"
     gene            complement(10168..10926)
                     /locus_tag="P5293_00030"
     CDS             complement(10168..10926)
                     /locus_tag="P5293_00030"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HRR48354.1"
                     /translation="MHINSDINILGGLTDWNLIKVFLLEDNDSINEKGGVHTFTAIKT
                     DKSVKRFEKAIRTTLLSNKRSDLEIIIRRAIKISAINTDTLLLLFWNASSNNELLFHL
                     NDKVFFPAFYSGRASIKSNEVEACLNELKQTEDELKKWSDITVTTTASKYLTLLKKFG
                     LMEGSVNKRIVHPYLSDAMFILFAYWIAAISQKPNLVNSHWLHYCFSEKQAFLDRLLQ
                     KKFSKYFNVVYTGDKLSIEPIIPYELIYEQITKS"
     gene            complement(11007..12131)
                     /locus_tag="P5293_00035"
     CDS             complement(11007..12131)
                     /locus_tag="P5293_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRR48355.1"
                     /translation="MNNIDFKDGYIPDILIVDDMPANLVLLGHILKNVGYKVRPVPNG
                     QLALKVAEKEKPDLIFLDIMMPEMDGFEVCRKFKENPDLNDIPIIFISALNDTENIVK
                     ALNSGGSDYITKPFKAEEVRARAATHIKLYLQAKELLKANAEKNKFFSIIAHDLKSPF
                     SNFLAVTQTLEEMLPQLTTDEIRSFASSMHKSATILYRLLENLLQWSRIQRGLITYEP
                     REFQINSLITESVSMYKDTAKNKEIEISLYVPENLKAYADVNMLQTVVRNLLSNSIKF
                     TPRGGKIFINAKTLDEEYIEISIRDTGIGMPPAIADNLFRIDVKTNRDGTEGEPSTGL
                     GLLLCKEFVEKNKGKIYLESEENAGTTFHFTVVGTVQNFV"
     gene            complement(12128..14617)
                     /locus_tag="P5293_00040"
     CDS             complement(12128..14617)
                     /locus_tag="P5293_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR48356.1"
                     /translation="MNKKILNCKGLQRTELIVLLIILIGGAIYIFETWDKFSSRRNEE
                     ALQIARTAKASLPLYLLQDLKASPEDSVSHSYKLLKDALIRVARTNPKVSFAYLFAQK
                     NGKIYFYADSELENSPNYSPPGEEFYEADYEDRLPFITGKELITKPMSDRWGKWVTIY
                     IPVINENTGTINAVFGMDFDSNTWNNALHMDILRSSLFVLLILLTWFFQYRVLIKNRA
                     LKSLLQTTAKAEKALQSSETRMRSITNSANDAILMMDQTGAITYWNPAAERIFGYTES
                     EVLGKDLHHLLAPEKYHEAFKNAFPRFAETGEGSAVGKTIDLEAIRKDESVIPVQLSL
                     SSLKIDGNWNAVGILRDTTERKIIEESLIKAKQDSEAANKAKSVFLSNMSHEIRTPLN
                     AIIGFSQLMSREKDLNDRQKEYINSIIKAGEHLLELINEILELSKIEAGRIVLNPSTI
                     DLNSLCEDIRMMFSEKAKEKNLQLIFECTNELPQYIVIDEGKLRQVLVNIIGNAIKFT
                     EEGGVAIRVQTNRDKNGKYYLIVEVQDSGPGISENELGKLFKHFEQTNAGVKQGSGTG
                     LGLALSKELAQLMGGDISVSSKTGEGSIFTIKVEYNEGNLDEIEKLETKRIISIANHD
                     KKYKILVVDDKVENLKVTVNLLNAVGFETIEAVDGKDAIDKLNKTRPDLILMDMRMPV
                     MDGYEAIRMIKSMEEFKDIPVVALTASTFEDEKKRIEALKIEGYIRKPFRENDLFNII
                     GNLLKIQYVCESDQDDKNNNISGNKDVIQLSQKQISEILEALSIADMDKVLAIIDQIE
                     LIYPQFAYELKILAQNYDYSQIEELLKNKLK"
     gene            complement(14663..16498)
                     /locus_tag="P5293_00045"
     CDS             complement(14663..16498)
                     /locus_tag="P5293_00045"
                     /inference="COORDINATES: protein motif:HMM:NF027574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRR48357.1"
                     /translation="MKYTIHSLSTLSILILISTIIVSCYKDKIENLNKNQTAEKEVEI
                     KISLLKKGETIISKVSNVNDSSIRNVNIFIYNLSGNLIKSYFFNAESNLVITCSTGYK
                     TIIAIANCDNINYASYSSLDNIEEAISTQMTNSFGEFVFAGKTYYKIEPGKSISIDME
                     RLVSKITVLFDKKDLDPNTTIDIESISLRNVPSGCKFLRPNKPVFSDEINEYGESITE
                     NLEPLSHATATPLFMFENMQGTIGGATVESEKNPGNSAPFCSYLEMKANYTSQYRSGK
                     IKYKFYLGNNLINNFDIHRNVWYQLNVIFRGSAINEISWRIDTTEMVDIFYLIEAVPN
                     PFEGGTVSGEGYYKYGTMPTLTAQPNTNYKFIGWTPPLNAVSSDKLYTANFQYEIPVI
                     HVTDITVSESSVNIDRGISYQLFANILPANATDKSVIWTTSNPNIVTVNQSGKITGIN
                     AGTAMITVTTNDGGYSSSCNVNIYKVTRLFFHFERIAKVEFKNMNGGYWQLSEPMTDN
                     VYVMSDGEDGIPGTITYSIDWAYDPSSGFSPPERTSGTKNIIINNSLNTLLESGCAWG
                     SETDFSISYPTIEVHSISPTFTVNPLTRVIVDQNLNNYTYTEKGR"
     gene            complement(16495..18360)
                     /locus_tag="P5293_00050"
     CDS             complement(16495..18360)
                     /locus_tag="P5293_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR48358.1"
                     /translation="MKYKKITILFFIIAISSCNKQLYNIDREHNTILVTHADKSEKSS
                     DVKLSRREISNNAISHEIDTYAGGYLYEAVRDEKSGEMISVKQLDEITISSRSYNISE
                     RNGSISLGFVVTIPASLMNSRWQIVLLPILSRGDDTLHFSKIVVKGEDFKRAQEKSYC
                     RYEKYLHKIIPDDADFYTFYTDLPNLMIFLERNLPKSRIFEGHFNEELQSEFGISEKK
                     IIDHYVKNWLIEKNEKRKREKDLYFNKYVKNQKIKNCKLDSVIKSSNGDFAFHYTQEI
                     LANEQSSKLYLNLYSTISDISGFSINIKPSDTLIYNVSSMVSLLDTSKKYTKKIVFRK
                     VSTTKSLSITFKSGEYKLDPNLSGNLDQLQMIKQYLDNNNSNELKLDSLIITATASPE
                     GSYKSNKILSANRAASVVKYFNDILKDVKIISRSIPEEWSKLSLLIQNDSNIIERPKI
                     MECFKIDDPDKRELAMMLFKKDYHHIKEELYPLLRKVDFTFYHHKTNMIKDTIHTSEI
                     DSNYMNGIKLLKERNYQDAVKILTAYNDVNTAIAYMSLGQNAIAEKILAKLPENAKTI
                     YLMSILASRNNQEEKAVKYFLKAKQMDISIAYRGTLDPEISYLIRKYNLNDDLFK"
     gene            complement(18370..18906)
                     /locus_tag="P5293_00055"
     CDS             complement(18370..18906)
                     /locus_tag="P5293_00055"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRR48359.1"
                     /translation="MVKKILVFIIMFIITLMTCKAQKYGIGTNILGLGCGSINFEASI
                     ATSDKITFNLPFSWNPFELSKNNKIKHLCIQPGFRKWLWHSYSEMFVGSCVVASLFNF
                     AFSKERKEGYCAGISFNCGYAKMINRRFNLEFECAGGAMFFNYDRFEKKNCGEYLGNV
                     KKVKLRPTKLSFSLVYIL"
     gene            complement(18900..19820)
                     /locus_tag="P5293_00060"
     CDS             complement(18900..19820)
                     /locus_tag="P5293_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HRR48360.1"
                     /translation="MKLVSVAIILIGTFSSLSCIKEEKDSCRQCENKLMKVSFKGSYM
                     REGITKSSSLMPEGIESIILAYNAGDNPTKKSSYPGTPLFVKSDINGYLTVNEDKNIM
                     MPIGNYDFYAISTNSNFMESLILKDGMYEGLTNGKDYLWASQKEMKIKSDTDITFNFA
                     HKATAFILNFYSGKGIDSLFISKIFINQCLEGGKLSLVTGKIIPSNCLSAEIITVKAE
                     NNSCKWIMLPLSGKIDIPIIVDVLMQINGKIISKKIGTSIPAMDNGFLEGVIYVFNII
                     VDTESMSFSQTSVEGWNEEKCANLYFNEPW"
     gene            complement(19828..20718)
                     /locus_tag="P5293_00065"
     CDS             complement(19828..20718)
                     /locus_tag="P5293_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HRR48361.1"
                     /translation="MKSIKIFYSIIILLLISTSCQKENLNKLQKEEIKFYASYSADTQ
                     TKATSTFASGNKVTIIGYSSEANTASAPTVAGTPIDATCNSSGLLTPASPIYLPKGNY
                     DFYSVSLNNSTAPEINFSSGISGQLTNEVDYLWAKCTNIANGGNVSFVYWHKAVGIEI
                     NITSGIGISNLIVTSISFTPTAPSSISKMKLSDGSIESANSVGSLTAMALNSNKGKFI
                     MLPVNAVNLNVEVIVNATIGGTPVTGKKYTATIPAQNYLSGSYYMVDFTISSSAMQFT
                     GTLIEDWTTQNISGVTLSEQ"
     gene            complement(20742..21554)
                     /locus_tag="P5293_00070"
     CDS             complement(20742..21554)
                     /locus_tag="P5293_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48362.1"
                     /translation="MLSSCIQEDYDNCPDYGKYPVIFIDDDTNLQREDYRVIIKVFEQ
                     NKSSHVVTVNYIIRAGERELYSDKTLKLYPGYYTFKAIKSNNILNIKDDLLRMVNGYA
                     CLSIDLSEKIKKAPKNQLNMKFKLSNSLIRIACNMDKEAKNLFEIINVEISAPDDSNV
                     FLNTITGKSNYSQEVTEYYDSCHLETEPYIYYYYSVPLVRSKYLNFKFTFKELSTRKV
                     SSIYTRVFLSSDIEQGKVYDFHFDITPQDISYKITTIMDWETLVYDPQIHIN"
     gene            complement(21572..22429)
                     /locus_tag="P5293_00075"
     CDS             complement(21572..22429)
                     /locus_tag="P5293_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrillin family protein"
                     /protein_id="HRR48363.1"
                     /translation="MKKAWALLFSILIIIGCHQIDVPYVKQLNNINFKCSVIYPDVKS
                     SQYFGYGKYATIYAFDTGDLRNYVGNTPVSAFSSGYGQLVTLYPLSISDGIYDFYSIS
                     ENSGNSPQLYFYDGQASGVVNGIDYLWAASKEKNITYDRNVEFHFKHVSCQIKLTILS
                     EGNVSSVNVTDVKFTFPSQDGASIYLSEGYIAPVNNVGALESINGYAHSMNFYLLPCT
                     ASMIIEVVLDASIDGDFQTGRIYRATLSEDFIGGNAYELCLTVKNNNTISVNCKTKAW
                     STHTESITY"
     gene            complement(22436..22918)
                     /locus_tag="P5293_00080"
     CDS             complement(22436..22918)
                     /locus_tag="P5293_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48364.1"
                     /translation="MEQSLSFEKKTSQADSAKNEINPDSKYVQVYFHIKRVAKMHNKI
                     SYDEFGRKHVCELADEITDRIYVRCLGSSKGYKVIYDLEVICGNDPTTYLKNVETYVT
                     KGLDNELAWIDHMITNCNDIFPIINITAFHPFKISETGTESVLYNPLLNSCELIKLVN
                     "
     gene            complement(23283..25178)
                     /locus_tag="P5293_00085"
     CDS             complement(23283..25178)
                     /locus_tag="P5293_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRR48365.1"
                     /translation="MSEIINHECGIALIRLLKPLEYYQNKYGSYLYGLNRLYIMMQKQ
                     RNRGQEGAGVVGVKLDMPPGYKYMDRARSCDPNPIQEVFNKINEPFVTENLKQRDAQW
                     AKMNLPFISEIYLGHLRYSTFGKNNIDLVHPLKRASNWRSRNLAIAANFNLTNIDELF
                     ESLIRAGQHPIFYADTVTLLEKVGYFLDDEIQNKYRKYRDEGYSKIEITPLIEKNLNL
                     ADVLSKSTENWDGGYAVAGVLGHGDAFVFRDPWGIRPVYYTVNEEFVAVASEASVIRT
                     SFKVNDSMINEIKPGYALIIKNSGEISETLIREPLKRSSCSFERIYFSRGNDSQIYKE
                     RKMLGKLLSKQVIKAIDEDIDNTVFSFIPNTAESSYYGMISGIQKYLVGRKTTQIINI
                     IHNGDKIDEKSLENIILKEPRTEKIVIKDVKLRTFITSDGDRDVMVEHVYDVTYGAVK
                     ETDNLVVIDDSIVRGTTLKQSILKILDTLNPKKIIIVSSAPQIRYPDCYGIDMTRMGE
                     FIAFNAAIALLKEHGLDDIILDVYMKCKNQVGLPKEKYINYVKKIYEPFTDEQIADKI
                     AELVKGNEIKADVQIVFQSIENLHKACRENSGDWYFSGDYPTPGGNKVVNTAFINWME
                     GCDDRSY"
     gene            complement(25181..28159)
                     /locus_tag="P5293_00090"
     CDS             complement(25181..28159)
                     /locus_tag="P5293_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRR48366.1"
                     /translation="MSEKESQNISSKMNYKILMQNRIRRVLMICTSYDAYILEEDGRI
                     EDQIYKEYVDLNLSNPPTFTWVNSSMDALKLLDSDDDFDLIISMINVGELDVFKFSKL
                     CKVEHPYIPFVLLSNFSRELERIVREEDCSAIDHIFSWHGNADLILAIIKLFEDRINA
                     DDDILNAGVQSILLVEDSIRFYSTYLPTIYRLVLEQSAEFLKETLNEQQQKLRKRARP
                     KILLATCYEEAVSLYQKYKQNLLGVISDVAFVIHKNDPPESEKIDAGIDLCKMIKNDD
                     FYMPFLLQSSQESMRNVAESMGVGFILKHSKTLLLELSEYISEEFVFGDFVFRDNNSG
                     IVYGKAKDLKDLQHLIMEIPEDVLLYHASRNRLSKWMYSRGLFDLANKIRDVREGQFN
                     SINELRNFIVKSIIEYRMVVGHGVVAKFNKETYEEFIWFARLGEGSLGGKARGLAFIN
                     SMLQKHNLLNKYPGVKILIPRTIVIATDYFDEFIKENGLQYVINSEISDEELLSEFTS
                     SRLPARLIEDLSEYIKYVNGPLAVRSSSKLEDSHYQPFAGIYSTYMIPFTQNSDQMLR
                     LLGKAIKSVYASVYYASSRSYIQATSNLISEEKMAVVIQDVCGTEDSGYFFPTLSGVA
                     RSVNFYPIGDEKPEDGIVNMAFGLGRLVVEGEKCLRFSPKHPKHILQLSTPELALKET
                     QNHMYALNLKPEEFKTSIDESVNLAKFDINQIKHFRNMQHVASTWNAQDEMLIDSNLV
                     EGRKVITFAHILKHDVFPLADIICDILNISQTEMRTPVEIEFAVNMDVDAGQDIIFNL
                     LQIRPVADNQDYISINWDKIDKNDCIIYAEKALGVGNIEGITDIIYIREENFDNSRTK
                     DIAAEINYLNKNLKEEGNNYILIGPGRWGSSDSWLGIPVKWSDISEAKVIVECGQKNF
                     HVEPSQGTHFFQNLTSLGIGYLTINPFLDDGKFDREYLDSLEAVHESNYLRHIRFKVP
                     LKVCIDGKNSRGIVIRPITK"
     gene            complement(28174..29025)
                     /locus_tag="P5293_00095"
     CDS             complement(28174..29025)
                     /locus_tag="P5293_00095"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRR48367.1"
                     /translation="MKICVIGSGTMGSGIVQAFSQSGFNVIMKGLNDAELQSGFKKIN
                     SNLSRLAEKGKIDKSEVESIISRIVGTTSYDICKDADLIVEAVFETMEVKNEIFKTLD
                     EICDPKTVLASNTSSLSITEIAASTKRADRVIGMHFFNPAPVMKLVEIIKGQLTSVET
                     QQFVADIATKAGKTPVNVNEAPGFVVNRILIPLVNEGISILADGVATRDEIDEAMKLG
                     ANHPMGPLALGDLIGLDVCLAIMEVLYKEFGDPKYRPHPLLKKMVRSGQLGRKTGVGF
                     YDYRKQQ"
     gene            complement(29043..29822)
                     /locus_tag="P5293_00100"
     CDS             complement(29043..29822)
                     /locus_tag="P5293_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRR48368.1"
                     /translation="MTYKYLNISIDNQIYTLTISNPSQLNALNSYILKELDDAIDNLS
                     KNEDIRVLIITGEGKSFVSGADISEMVSMDGIEAAEFGYFGSMVFRKIELLPVPVIAA
                     VNGFALGGGCELAMACDIRIASENAKFGQPEVGLGITPGFSGNFRMTRIVGIAKAKEL
                     IFTGKIINSTEALSIGLVNSVVPQETLMENAIKLANDIAKNAPIAVRNAKNLINISAD
                     LTVDEAIKNEIQYFAACFGTEDQKEGMKAFLNKTKVKFNNR"
     gene            30062..31558
                     /locus_tag="P5293_00105"
     CDS             30062..31558
                     /locus_tag="P5293_00105"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRR48369.1"
                     /translation="MKEKLYVFDTTLRDGEQVPGCQLNTIEKIELAKALELLKVDIIE
                     CGFPISSPGDFTSVVEISKVVTESVICALTRAVDKDIEVAADSLHFAKRKRIHTGIGT
                     SDPHILYKFKSNRDEILERAVHCVNYARKFTDDVEFYAEDAGRTDNDYLARVVEAVIK
                     AGATVVNIPDTTGYCTPEEYGKKILYLKNNVPNIDKAIISTHCHNDLGMATANTLAGV
                     MNGARQVEVTINGVGERAGNTALEEVVMAIKCRKDFPVETSIDTHLIIKTSRLVSTLM
                     RMPVQPNKAIVGRNAFAHSSGIHQDGVLKNRESYEIIDPNDVGINESLIPLTARSGRA
                     ALKHHFLRLNIDLTSEELDVAYEKFLRLADVKKDIHDEDLLMISGVTKNIQTHKIKLK
                     YLQVVCGKSAIPMASVKLLIDGEECTATSSGNGPIDAAFSAVKSLINRKIYLEEFLIQ
                     AITRGTDDVGKVHIQIENKGNIYYGFSANTDIITASVEALIDAVSKII"
     gene            31580..32971
                     /gene="leuC"
                     /locus_tag="P5293_00110"
     CDS             31580..32971
                     /gene="leuC"
                     /locus_tag="P5293_00110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRR48370.1"
                     /translation="MKKTLFDKIWESHIVKEITGGLSILYIDRHYIHEVTSPQAFSDL
                     DRRGLKVFRPYKTFATCDHNVPTLNQHLPVQDSSSRIPIERLAENTSRHGILYFPLQD
                     RHNGVVHIIGPELGLTTCGMTIVCGDSHTSTHGAFGNIAFGIGTSEVEMVLATQSILQ
                     FKPKQMLINVNGKLTKGVCAKDLILHIISKLGTSAGTGYFIEFSGDTISSLSMEERMT
                     ICNMSIEMGARGALIAPDDITFRYLEGKEFAPKNEAWIESVNMWKLLKSDEGAEFDIV
                     YNFNAEEVEPMITYGTNPGMGINVNGVIPQNAKDGDSFSKALAYMGFVRGEKIIGKKI
                     DYVFLGSCTNGRVEDFRAFASIVKGRKKHPDIIAWLVPGSNGVLRAIEEEGIGDILRE
                     AGFEIREPGCSACLAMNEDKVPSGKYCVSTSNRNFEGRQGTGARTLLAGPLVAAATAI
                     NGVITDQRIYLIS"
     gene            32968..33561
                     /gene="leuD"
                     /locus_tag="P5293_00115"
     CDS             32968..33561
                     /gene="leuD"
                     /locus_tag="P5293_00115"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRR48371.1"
                     /translation="MSFEKFIELISEAIILPVDNIDTDQIIPARFLKTTTRENFGKNL
                     FYDWRYDQDGEPIESFILNNSDNKGRILVAGNNFGCGSSREHAAWALYDYGFRAVIST
                     FFADIFKNNALNNGLLPIQVSRAFFQKIVDNSHSLLDINLPMQSVTIISTGDSELFDI
                     NTYKKDCLINGFDDIDFLLSNIGEIEKFEQIKSTSRL"
     gene            33558..34634
                     /gene="leuB"
                     /locus_tag="P5293_00120"
     CDS             33558..34634
                     /gene="leuB"
                     /locus_tag="P5293_00120"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRR48372.1"
                     /translation="MKKKIAVLPGDGVGPEIIEQSIKVLTAVEKRFGHSFEFAYGSIG
                     ACAIDEFGDPYPENTHKLCLESDAILFGAIGDPKYDNDPSAKVRPEQGLLKMRKDLGL
                     YANIRPVIIYSALLDRSPLRTDIIKNVDLVVVRELTGGIYFGEPRGRSEDGRKAFDTS
                     IYSDFEIERISRIAFNLARKRKGQVTLVDKANVLATSRLWREVVSRLHNEEFKEIKLD
                     FLFVDNASMQLVLNPSKFDVILTENMFGDILSDQASIIPGSIGLLPSSSMGVYTPLFE
                     PIHGSFPQGAGKNIANPIATILSAAMMLDDAFNMVYEALSVRKGCEAAINNGKLTIDL
                     TDSKEACTTSQIGDYVADFIMEAP"
     gene            complement(34618..>35524)
                     /locus_tag="P5293_00125"
     CDS             complement(34618..>35524)
                     /locus_tag="P5293_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRR48373.1"
                     /translation="VLWAAFWNFAAFFLFGVHVANTIGKGIVDPNVVNPFIILSALTG
                     SVIWVYACTRLGMPISVSHALIGGLIGPVWFTHGSSALIGTGILKVGIFIVLSPLIGA
                     FLGFLMMALTMWIHKKKHPMKVEKNFKVLQLFSSAVFSLGHGSNDAQKTMGIIAVLLF
                     SVAGTNDFIAKYLYEPNAGFHIPLWITITCYSVIALGTITGGWRVIKTLGDGLAKLKP
                     VQGFCAETAGAITLIGTALSGIPVSTTHTITGSIIGVGMTKGIASVRWATAKNIVGAW
                     IFTIPATLLISGGIYLIIDAIMVLP"
BASE COUNT        10387 a         6504 c         6342 g        12291 t
ORIGIN      
        1 ttctttgtat ttgccctctt tctccaattc ttcggctttt tgtaaagcga cattatattt
       61 tttttctttg gcttcctttt cctttttctc cttgtcttgt ttttcctttt ccattgccga
      121 tttctttcaa tgcctgtatc accaatcggc tgattttccc ttttgccaac aagtttttgg
      181 gggttgtttt tttgaatttg atttttcgtt tcccaacctt aatttcccga gccgaaccgt
      241 ccgtgagcaa tacgatgttc atgggcactt gggtactcaa gcctaaagca tttaaagcat
      301 agctccctgt tggaaccgtt cttatgcgat ctcgcttagc tatggcttct gctatttctt
      361 cggctgaggg tatcagcttt ccgattatgt tactctcttt cggacgaaca taaattccct
      421 gtgcgactct cattattaca ccctcttttt ctaaacgaaa aagtgatagc cgaaccgctt
      481 ccgatgatcc cagttcacta aaatcggcag gaagaaacag ctctcctttt ggaaaggctt
      541 ctatacgctg caatattttt tcttctatgc tcatcatctc aattttgtaa caaaaatagt
      601 agatatttgt tacaaaacaa aatttcattg tagcaaaccc caatcaccaa tccgcattta
      661 aatatctctc taattgtgat tttttgccac cggctgtatt gagcacttca gctctcagca
      721 aacctttctt ctgcaatggt gcaatgtttt tgcctacccc atcatccagt ttaggatcgt
      781 agccctcggc tatgagttcc tcaattttgg ttttgaaaac ttcaatttct tgcaactgca
      841 aacgaatttt ttctttctct gcttgtgcct gtgcggtgtt cacatcctgc aaagtaattt
      901 ggcggtactc taagttttgt acccgttggc tgatgtaccg cgatttgagc ttaaacaggc
      961 tgtctctgtt ccatttgtag atgaggatat acgcttcaaa gccctgattt cgcccactgc
     1021 tcagatgcca gataaagggc gttttgggca ggtacataaa caggttgagg tggttgctaa
     1081 gctgattgaa aaaatgatgc tccagatact cattgatggg gcggccgagc aaactatcca
     1141 gttgcatata ttgggcagag gtgaagccgt tttgcaggca gtgcttctcc aaacgctggc
     1201 taagggcttc ttctccgggc aaaccaacca agggtatgat tccatcttca tcctgctgca
     1261 acagggtgcg aatactatct gccaaaaact ccagggcgat ttctgcggcc cttgcctgtg
     1321 ggacggtgtt ggtttctttg aaccaatacc atacattgat ggggttgacc tgatggcgga
     1381 tgcaaaactc ttccaaatcg ttattgcttt ggtatagcgt ttcaaaacct tccttgattt
     1441 cccttacttg ctgttcatca aaagtagtaa cggataagtt ttgaatatgg tccattactt
     1501 cagtgagtgg attttctaat tgtgataaaa aagcgtcttt agcctctgat gataccggca
     1561 atgaaccgat ggacttaccc attttggctt ccacctgttc ccggtcagca tcgctcagtt
     1621 catatacttc aaaaatcaat tcgtcgatga tggcttcgtt gatcagcacc tgtgtgagtt
     1681 ggtcattttc ataattaaga tatgccaata cacggtcttt gagggttgca ccttgaaatg
     1741 ccaataaggg actttgggta tagtaggttt cagttatcct ataagagcaa aggtgttttt
     1801 ttattgatac attttctaag ctaatagctg aaatattttc ttctaaaatt tgagaaggtt
     1861 ttataaaagg aattctctga atatcaccaa ctgtgatatt tacagtaggg tttaacattt
     1921 cattgacata gccaataagt gaactattta agatagcaag aatataataa tagttctttt
     1981 cagctttgtt tttaaagaat atacccaaat cggcattact gaaaatagaa tctgatggtt
     2041 taaatctaaa agatggtccc tttgttccaa ctgatggaca tacaatacct tctcttaggt
     2101 aattagactc acctgaaaag cctggactac tttcattttc aatatttctt ctttgatttt
     2161 gaaaatcaat tgtcaaccaa atattaccaa accacttatt gtatggacca cctttgtata
     2221 tttttgaata ctttgaattg aattcataat taatttcaag gggtgagcgg aagtatttaa
     2281 tattattaga tgtttttaat ccttcattta tttcaaagat tgctttcatt gtaatacctt
     2341 taaatttctc cctaaaccca tcacttatcc aatagataaa aggccagcct tcaatgattt
     2401 tgagtttttc ctgtgggagt gaataaagtc ttggattggg tcgattgttt attgcatcct
     2461 cgtaagcttg ctttaatgag gcttgtttgg ccttttcttg ttggttagct gtgatgttaa
     2521 agtagatacc cggcgtttct ttcttctttt tactcaacac ataccaagta gcatccaaca
     2581 aaatacccgg gccaaaaaga ttcactctgt ctaagccata atcaaccatt atgtcaatgt
     2641 gcgtattttc aataaggtat ttacgcacat cttcaaacgt tttaatgaac ataaacgtat
     2701 gaggatgtat catccccaca taaccatcat cggttgtgat ttcgcaacaa cgtttgataa
     2761 atgcagcata taaattggtg ttgaacttat atggctgttt gtaattggct tctacaaact
     2821 tcttcagttc cgggccaaag tcggcactgt cggtataagg cgggttggct acggccacat
     2881 catatttttg ggtgagtagt tgcaagaaag taatggcatc ttgcgttttg ttgttcaaga
     2941 aagtttgacc ttgcctggcg gtgttttgag ctactgcttt ttgcaggttg gtaaagaatt
     3001 gtgctttaaa tgtttcgtag ttttccactt cttgtgcacc aaaaagtgta agtccgtctt
     3061 gctttatacc gtgcaggtga atacttagtt tttcttcgag ccgtatgagc gaaccaaact
     3121 tgtaggcttg ttgcaggtcg ttccaaatat ccctgatgat ttccttttgt ttggaatcga
     3181 gcgtattgcc tcgttcaaag atttgttgta cttcttcata atcaggcagg aaaaaatcgg
     3241 aacttacgat attgaaatgc tcaattttta cactccgctt ctttcttttt gctttaatgt
     3301 acaaacccaa ctgagccaac tgtatggcac ggtcgtctaa gtccacccca taaaggttat
     3361 tttcaatgat gagtttggga atgtcggctt cattgtagtc tgctccgtag ttttcaattt
     3421 ggtccgtgta caaatcgtaa aacagatcaa agccgtagag taaaaagttg cccgatcccg
     3481 tggccggatc aatcatcttt atttcgtgga gtggttttct ttctcttgtt tgtgttgttg
     3541 gagcattcgc aatcttatac ttgtggccta tttcactatc gggatacatt tccagataga
     3601 gtttgcccag gctgttgtct accaaaaact gcacaaccca tcgaggggta tacacctgac
     3661 tttggatgct taccttgttg tattctgttt tttcgccact ggccttgtga gctgcttttt
     3721 tggagttgtt gtagctttca tagagccaac caagtatatc atcgcttctc cagatatctg
     3781 cttctacctg tgcatctgtt tctacctgat tgaatgcatt gatgatgctt agtagttcaa
     3841 gggccgtagg cagcaaatga taaggatgct gtggactgaa caagggtata tcgacggcca
     3901 atttttgcaa ctgttcttct aggaagggaa gcaatccttc ggcctcctcg cttcttgctt
     3961 gcgggttttg ctctaaccat gccaaatgag caaaagaacg accaccgtgg ctttctcttc
     4021 gggttacaat ttcggggtgc agggtatggg cttccatcac tttgagggct gccaaacgat
     4081 tgaacaaagt gaaggtgagc tcttccacca gtttttcgta ggcatcggaa atgcttcccg
     4141 tttcagcaat aaacacttca cggatggttt ccatacgtat gcgttcagca cggtattctg
     4201 atggaatggt ttcaatatcc gaaagtagat tttcagaaat acccatcctg cccaggcgat
     4261 tacggaatgc ccggtctatg agttggcgta tttgttcggt atgttcggat agtttcattt
     4321 ttaaaattta tttaaaagac cacctaaaaa cgctttgcct tgttgggtta atgtgtattt
     4381 gttatcagga tcagtaggtt tttcaggaat ggtaaaaaca accaatcccg aatctcttag
     4441 tggtttcaga taattatccc taaaagtttt ggcgttttta taatcaaatg cagccatcaa
     4501 atcagaaagg gctatgggca agctgcacag gcttaaaatg ccaatgaggt accggttttt
     4561 ctttttgagt agctgggtac ttttttcgtg ccagccgggt acctttttta tttcgtcttc
     4621 tgcaaacact tggttttcaa accaatccaa agcattgagc tgggtacctt tttggtgcca
     4681 gcagggtacc aaatgcaaaa gcacttgttg ccaagtctgt tcaaaacact ccgcaggcag
     4741 ttcaatttgt tgcaagcctt ccgacaaatg ggaagcgtag ctctcgctga tacctaacca
     4801 tgagataaag ctatcggcat aattccccag gcttacaaat cgtaaaggaa taaccgtttt
     4861 gaagatgtcg ttttccacaa acagaggatg agcccccggc gcataaagcg gcagatattt
     4921 attggtattt ctaacccccg atccaatttc gtcagtccat ccaaaagccg taaagaattt
     4981 acggatattt gggttcttag gatagggatt gaagccaaag ggatcaatta ccccgtggaa
     5041 gtatggtttg tttgggttgg taattaccac ttcagatgct gtgatgatca attctgtcga
     5101 caaggcgctg gtatattcac ggtgtacaat ggcattgcca atgacttctc tgaatatttt
     5161 atcccgtaag tccacccgct gatcgccttc cataaagaat ttttcgggca gatgtttgtt
     5221 gataaaagct ttcaattcaa gataggtatc aattaaattg gtacgcagtg gagggttaat
     5281 ccggtcatcg taacggtctt tattttcttt tctgagcatt ccttccactt tgtaggcagg
     5341 gagcagactt tgaatggttt catcttttcc aaaaattaat gctgcggcta aggttaatcc
     5401 gtcctgtccg gtttggaaat ctttcctcca aagggtggct tctctgagta attgctcttt
     5461 gttggccata agccaagggt gatcagactt attattgcgg ataagcgtga atgctttatc
     5521 aaatagctta tcatctaagt cagtcattga taaatgcgga tatataatgg cttcagtgaa
     5581 aaaattgcgt ttacgcaagt ataaatcgct caaccgttgc tggttattag ttatgtctaa
     5641 atcactttca aaatcacggc tatatatcgt tcctgcatgg ttgtgaatct gactacttgc
     5701 aggaatctga atcaccataa ccttaccatc cgggtgattg atacaaaagg gctgtacata
     5761 aacaggaggg tctatgcaat ctctggaatt taatgccgta ataatgtcct tcattaacga
     5821 aacttcagca ttcccatcaa ttcctttcac tttaccatca tcatccaccc ccaaaaggat
     5881 ggttccacca tcggtattgg aaaaactcac aactgtttca taaaatgaag aaggcacact
     5941 gtccttcgca tctttaaatt cgatacggag accttcgcct tgattgatga gatattgtaa
     6001 gtcatttatt tccatttact aagcgtttat ctctatttcg tcattgtctg atgcaccggc
     6061 caatttttgt aattcgccat gcaaccaaat tttatattca gataccttca tttttgtagc
     6121 aggcattttt accgcatatt ttttcactgt gggcaattga aaagttccgg gttgagaatc
     6181 cggtgctttt gttttgacta atccggctct tgcaatttct atctctgcct ttttgctttg
     6241 gtataattca ataaaagaaa gcacttcgga gtaggtgaaa cgactatgtt tatcttttac
     6301 atcgaagtca atattcacgg tagcgatttt tctttgcccg gcatattggt gaagggctgt
     6361 agctttagac agggttgcat cattcagtcc ggatggcaat gcttcaattt ctttgatgag
     6421 atcacttgcc agagtgtcta tctctgtgta tttagccgca cattctttca ttgcttcact
     6481 aaacaattga tggtatctat ctttggtttt ttgggctgat tcttgcaggg atacaaaatt
     6541 tttaatgaca tctccttgaa agtatatgtc aaaatcctct tttaatttct gaatggttgc
     6601 gtctatttta gccgctttgt tcaattcatc ttgcacggct ataacaaatg ctttccacac
     6661 tttcactttt ggtaagttgt tgcggataaa cttttctact ttttcaattt ctatcaaggc
     6721 cttgtagaaa gcatctttcg aagccaaaaa ctcttttgct ttgtcaatgt agttagcttc
     6781 actcacggct cccgtgaaac cgcctaaata agcggcgttt tcctctgccc gtgggaacaa
     6841 cttgtcaaat tccttttcgc tgtttctaag cgtggtgact ttatctgcaa aatgcctggc
     6901 catatcacgt acagctgaca ccaactcgaa atcattggta ttgtaatcta ttttacggcc
     6961 aatatatttc tccacctcta cctctaacag aaactgggcc aactcttgct tttgcaaggc
     7021 agaaagtgat tttgagactg ctttgaaaga tgcttttctg aattcacggg caacattgaa
     7081 aatggttgta actccttcat cacgccagga gaatttttca gctccattat atttggcaat
     7141 cagtttcccg gctctcatta atgcggcaac agacgatatg acagtgccaa atgtgaaacc
     7201 ggtaggtgga tgttcgagat cattttctaa tgtggcacca tccacaaatg tgtttcgtac
     7261 tttgtataag atttcttcgg atactttcag gttctcgcca atgaaattgc cttgtgtatc
     7321 aaaaaaagca aaatcgctgc ccgtaaaata ttgatgcaac cggccactgt tagcttcctt
     7381 tatcactgcc gcagcgactg tatcgctcaa ctgtgaagcc aaacgtttgc tgtacacgtt
     7441 ttggatgatg ctcctttgtt gagcttgcag cgttgtttgc caattatcat tatcgagttg
     7501 aaatgtattg tataaataaa tggcagttga attgaacaag gattcttcta ccagctcttt
     7561 aatgcggttt tgcttttctc cttttgatgt ggaaaagctg atgagtattt tgccttcttc
     7621 agaattggga ttgttatatt tctgctccag gtacgtgatg cgttccactt cctcgatcaa
     7681 tctgtccaac tctttgaaat agctgttgtc cggcactaac cataggaggt ctttatcgtt
     7741 ttggtgttgc actttcaggt tgtcaatgtc agcagaacga tcttcggata tattgtaaat
     7801 gctcttaatt tttactctga ggtatttgag gttcggtgag gttaattcat catcattatc
     7861 ggtggtaatg taaaaatcga attgcaaccc gttatctgat accttgccaa tggtttttgt
     7921 aaagtttgca ctcttgtagg ctgttaccag ttgctttttc tttacaaagc cctgtacagt
     7981 gaacccgttc atttcatcca acaatctttg ttcaatatca gaggtgattc gatatgtctt
     8041 gttggtatct aacagtactt ttgcttcggt gagtaaatca agtgccttta caatctcctc
     8101 ttgaactttg aactgctcat ccgggtcgct gatgaagcat tttatgatgt ttggtaagga
     8161 tgagggggta acatctgctc ctgtaaggaa attgatggtt tccaataatc ttctgccgga
     8221 aatggtcgat cctgattcca ataaaatccg ctcggcattg tcatacctgc ttactaatct
     8281 gaccggtggc tgtggttgtc cctctttggc tatgtgccaa ccggtaaccg tatggaataa
     8341 gtctgtatgt tgtacttctt gtttcaggat gtcataagtg gttatgatca tacctcttgc
     8401 cgctactttg gtagaggcat agcctttggt gccaaacagg aagttttgaa gcaaatcgaa
     8461 ttgatacttg taaaaagggt agtaagttgc ataattgtct gcactatcgg ttttgctgat
     8521 acctgccccg tgaatggcgg catggtcggc aatctttcct gaatttttga ggaaatgatc
     8581 agtcagtttt tgaatgccgg attcgttttt ctttaatagc ctgttgcgga taataacatc
     8641 cacttcggta gcttcgaggt gaattttcgt cttgaaacgg tcggtaactt ttgttaattg
     8701 agctttgctt acattggagt tattgatcac atcatccaac ttttcttgtg cgattgcaat
     8761 ggtccaaact ttaccgccta atgacgaaag cgattcgctc aagccttcga gatccaacag
     8821 gctgattttt ttttggttaa tggcttcact ggcttcgtca aacagaaata ttactttctc
     8881 atttttttca acttgcaggt agtttgataa ttcttccctt aaacgtgatg tactaaactg
     8941 gtccatatct ctgcggatcg tagtcatcag gttgtcgtaa tctgattctg tgtttcctaa
     9001 attgatatac aactctttaa tggctttcgc gtatttaaaa gaacttgttc tgatctcatt
     9061 ccaatctaac cctgatacat caaaaataaa gtcttgtaca attgtttttc tttcgcttaa
     9121 catcaactga aacaagaaga taccgtgttc gttttcaggc aggtttagtg acctgagaaa
     9181 atttccgaac aaggtatatg ctatcccttt tgatgtgtct tgtttggcaa tatcaagaaa
     9241 tacgacccgt gaggttattg tattcaatct cgacaatgca ttttttacca gcgcttcatc
     9301 atttattccc gtgaatcgtt gaatgattct atctctggcc ggtgtgccac caatattggg
     9361 gttgctaagc atataaccca acaacttacc aaagtatgat ttccccgatc cgtaaaatcc
     9421 cgagatccaa acgcctgttt cgagtatatc ggatgtgaag gtatcaacaa atccgacata
     9481 ctcccttgcc aatccatctg ttacgatata actttctatt tctgcctgga tttccttctc
     9541 ggaaatatct tccaagtcga ttacattctt gatgtcttct gtcaggtcaa tggtcaatat
     9601 ttcttttatc ttcatttctg taattttctt tatttcacaa tcatacatct atattttgaa
     9661 gcagggcgcg tatttagaaa catcaatcgg tcgccttcct gggtggcagg gtataaaata
     9721 ataagcggca attttgattt catcaccagt tcattttcca tcagatgaat gttgtcaatg
     9781 ccactgccat ataaagcacc actgtggata agtatgggtg ttttttgctc gctgtaagtt
     9841 tgttttatgg ctgagagcag gtgtttaaat aagtcgggac ctgtttcgcc ttccggtgct
     9901 ttgaatattt gatgaaccga acccttcagt aaatcgaaca tttcggcgag gtcactttta
     9961 tatgtttcca caaacgagat caatatttca ttgatgtcaa tgatgtggta ggtttcctgc
    10021 ggcattagtt cggttaattt ttcaatgaat tcaaactcct gctcaggagg gcataccagt
    10081 aatattgaat tacccccatt ggcattcaat cggatttctg aacgcttgtt agactccaca
    10141 atacgcttta tctgtgaaat ggtgtattca ggatttagtg atctgctcat atattaattc
    10201 ataaggtatg atgggttcaa tactcagttt gtcgcccgtg taaaccacat taaaatattt
    10261 ggaaaacttc ttttgaagca gcctgtccag gaatgcttgt ttttcactga aacagtaatg
    10321 gagccaatgg ctgttcacaa gattgggttt ctgggaaata gcagctatcc aataagcaaa
    10381 caaaatgaac atcgcatcgc ttaggtacgg gtgaacaatt cttttattaa ccgaaccttc
    10441 catcaacccg aacttcttca aaagagtaag atatttggat gctgtggttg taactgttat
    10501 atctgaccat tttttcaact catcttctgt ttgtttaagt tcattgagac aagcctcaac
    10561 ttcattactt tttatagagg ccctgccact ataaaatgcc ggaaaaaaaa ctttatcgtt
    10621 gaggtgaaac agtaattcgt tattggatga tgcattccag aacagtaata aaagtgtatc
    10681 ggtattaata gcgcttattt ttatggctcg tctgataatt atctctaagt ccgatctttt
    10741 gttacttagc aatgtagttc taatggcctt ttcaaagcgt ttcaccgact tgtcagtttt
    10801 aatagctgtg aacgtatgga ctccaccttt ctcgttgatg gagtcattat cttctagcag
    10861 aaaaaccttt atcagattcc aatcagtcag gccgcctaat atatttatgt ctgaatttat
    10921 atgcatctaa tttattatgc tttcaatttt ttttgttttt ttctctatga aaatgtagta
    10981 tgtgaatttg aaatcgaaat tatcgtttac acaaaattct ggacagtgcc cactacagta
    11041 aaatgaaatg tagtaccagc attttcttcg ctttccagat aaattttccc cttgtttttt
    11101 tcaacaaatt ctttgcaaag gagtaaacca agaccagtgc ttggttctcc ttcagtgcca
    11161 tctctatttg tttttacatc aatgcgaaac aggttgtctg ctattgcagg aggcattcct
    11221 atcccagtat ccctgatgct tatttctatg tattcttcat caagtgtttt agcatttata
    11281 aatatcttac cacctctagg agtgaatttt attgaattgg agagaagatt ccgaacgact
    11341 gtctgcaaca tgttaacatc tgcataagct tttagatttt caggaacata gagagatatt
    11401 tcaatttctt tattttttgc agtgtcttta tacattgaaa cactttcagt aattaaggaa
    11461 ttaatttgaa attctcttgg ttcgtatgta attaaccctc tctgaattct tgaccattga
    11521 aggagatttt ccagtagacg atacagaatt gttgctgatt tatgcatgct cgaagcaaat
    11581 gagcgtattt catccgttgt aagttgtgga agcatctctt cgagcgtttg agtaactgca
    11641 agaaaattgc tgaaaggact tttaagatca tgcgcaataa ttgaaaagaa tttgtttttt
    11701 tcggcattgg cttttagtaa ctccttggct tggagatata gtttgatatg agtagctgct
    11761 cttgctctga cttcttctgc cttaaaaggt tttgtaatat agtccgatcc accagaattc
    11821 agtgctttaa ctatattttc tgtatcattt aaagcgctaa tgaatattat aggaatgtca
    11881 ttcaggtcag ggttttcctt gaatttgcgg caaacttcaa atccatccat ttcaggcatc
    11941 attatgtcca aaaaaatcag gtcaggtttt tctttttctg caactttcaa agctaattgt
    12001 ccatttggta caggtctaac tttgtagccc acattcttga gtatatgccc aagtaataca
    12061 agattggccg gcatatcgtc aacaataagg atgtctggta tgtatccatc tttaaaatcg
    12121 atgttattca tttaagtttg ttttttaata attcttcaat ttgggaataa tcataatttt
    12181 gggccaaaat ttttaattca taggcaaatt gtggatagat gagttctatt tgatcaatga
    12241 ttgctaatac tttgtccata tcggctatag agagcgcttc aagtatctct gatatttgtt
    12301 tctgagacaa ttgtattaca tctttattgc cggaaatgtt attattttta tcatcctgat
    12361 cgctttcaca aacatattga attttcagaa gattgccgat aatattaaac agatcgtttt
    12421 ctctgaatgg tttacggata tagccctcga ttttaagagc ctctattcgc tttttttcat
    12481 cttcaaaagt acttgcagtt aaagcaacaa caggtatgtc cttaaactct tccatcgatt
    12541 taatcattct gattgcttca tagccgtcca taacgggcat cctcatgtcc attaaaatca
    12601 agtcaggtct ggttttgttg agtttatcaa ttgcatcttt gccatcaaca gcttcaatag
    12661 tttcaaaacc aacagcattc aataggttaa cagtaacttt cagattttcg actttgtcgt
    12721 ctacaaccag gattttatac tttttatcat ggtttgcaat agaaataatt cttttagtct
    12781 ccagtttttc tatctcatct aaattacctt cgttgtattc aaccttaatc gtgaatatag
    12841 atccttctcc cgtcttgctt gatacagaaa tgtctccacc catcaattgg gcaagttctt
    12901 tacttaaagc caagcccaga cctgtaccag agccttgctt aacgccagca ttggtttgct
    12961 caaaatgttt gaatagcttt ccaagctcat tttcagaaat tccagggcca gaatcctgaa
    13021 cctcaacaat tagataatat ttgccgtttt tatctctatt tgtttgaact cggatggcaa
    13081 ctcctccctc ttccgtaaat tttatagcat tgccaataat atttacaaga acttgcctaa
    13141 gtttcccttc atcaataaca atatattgag gcaactcgtt ggtgcattca aatattaatt
    13201 gaagattctt ttcctttgct ttttcgctga acatcatcct tatgtcttcg cataaagaat
    13261 taagatctat agtagaaggg ttcagaacta tgcgccctgc ttctattttg gaaagttcaa
    13321 gaatttcatt aatcaactct aatagatgtt cgcctgcttt gattattgaa ttaatatatt
    13381 ctttttgacg atcattgaga tctttttctc tgctcattaa ttgagaaaaa ccaataattg
    13441 catttaaagg agtcctaatc tcatgtgaca tgtttgaaag aaaaacactt tttgccttat
    13501 tggcggcttc agaatcttgc tttgccttta tcaacgactc ttcgattatt tttctctctg
    13561 tagtatctct caaaatccct acagcattcc agtttccatc aattttcaat gaagaaagag
    13621 aaagctgtac aggaataaca gattcatctt ttcttatagc ttcaaggtca attgttttac
    13681 ctactgctga tccctctccc gtctctgcaa aacgtgggaa agcattttta aacgcttcat
    13741 gatatttttc aggagcgaga agatggtgaa gatcctttcc taaaacttca ctctcagtat
    13801 atccaaaaat tctttcagca gcaggattcc aataagttat agcacctgtt tgatccatca
    13861 tcaaaatagc atcatttgct gaatttgtaa tagacctcat tctggtttca ctggattgaa
    13921 gtgctttttc agctttggca gtggtctgta gcaggctctt aagagccctg tttttgatca
    13981 agactcgata ttggaaaaac catgtaagaa gaattagcag aacaaacagt gaagatctta
    14041 aaatatccat gtggagtgca ttattccagg tatttgagtc aaaatccatc ccgaacacag
    14101 cattgatagt acctgtattt tcgttaatta caggtatata aattgtcacc cattttcccc
    14161 atctgtcact catgggtttg gtgattagtt cttttccggt gatgaatggc aaccggtctt
    14221 cataatctgc ttcataaaat tcttctccag gtggggaata attgggagag ttttcaagct
    14281 cagaatctgc atagaaataa attttcccat ttttttgggc aaacaaatag gcaaaactaa
    14341 ctttgggatt tgttctggca acacggatca atgcatcttt taacaattta tatgaatgag
    14401 aaacagaatc ttcaggtgaa gctttcaaat cttgtaataa atataaaggg agtgatgctt
    14461 tagctgttct tgctatttga agagcttctt catttctcct agaagaaaat ttatcccaag
    14521 tctcaaaaat atatattgca cctccaataa gaattattag aagaacaatt aattctgttc
    14581 tttgtaatcc tttacaattt aaaattttct tattcacatt tgataaaata atattcaaat
    14641 ataagtctaa tttaactatt ttttaccttc ctttttcagt gtaagtgtaa ttgttgaggt
    14701 tttggtccac aataactctt gtgagaggat tgacagtgaa agttggagaa attgaatgaa
    14761 cctcaattgt tggatatgaa atagagaaat ctgtttcact accccacgca caaccggatt
    14821 cgagaagtgt atttagtgaa ttattgatta ttatgttttt tgttcctgat gttctttccg
    14881 gtggagaaaa acctgaagaa gggtcataag cccagtcgat tgaataggta attgtacctg
    14941 gtataccgtc ctcaccatca gacatgacat atacgttatc tgtcattggt tcgctcagtt
    15001 gccaatagcc tccgttcatg tttttgaatt caactttagc tatcctttca aagtgaaaaa
    15061 agagtcttgt gactttgtaa atatttacat tacaagacga tgaataacca ccatcattgg
    15121 tggtgacagt tatcattgca gttccagcat taatacctgt aatttttcct gactggttta
    15181 cagttacaat gtttggattt gaggtcgtcc atattacact tttatcagta gcatttgcag
    15241 gaagtatatt agcaaacagc tggtatgaaa tacctctgtc aatgttaact gaactttcgg
    15301 aaacagtaat atcggttaca tgaatcaccg gaatttcata ttgaaagttg gcggtataca
    15361 atttatcgct actcaccgca ttcaggggag gagtccatcc gataaacttg tagtttgtat
    15421 tgggttgtgc cgtcaaagtc ggcattgttc catatttata atatccttct cctgatactg
    15481 ttcctccttc aaatggattt gggacggctt caatcagata gaatatatct accatttctg
    15541 tagtatcaat acgccatgag atctcattta ttgcgctacc tctgaaaatc acgtttaact
    15601 gataccacac atttcgatgt atatcaaaat tatttattaa attattacca aggtaaaatt
    15661 tgtattttat tttcccactg cggtattgac ttgtataatt tgccttcatt tctaagtaag
    15721 aacaaaaagg ggcactattc ccgggatttt tttcagactc aacagttgct ccaccaattg
    15781 ttccttgcat attttcaaac ataaacagag gtgtagcagt tgcgtgtgat agaggttcaa
    15841 ggttttctgt aatagactca ccatattcat taatttcatc tgaaaatact ggtttgtttg
    15901 gtcgtagaaa tttgcatcct gaagggacat ttcttagtga tatagattca atgtcaattg
    15961 ttgtatttgg atcaagatct tttttatcaa agagaacagt aattttagag acaagtcttt
    16021 ccatgtcaat agatatggat tttccgggtt ctattttata ataagtcttt ccagcaaaaa
    16081 caaactctcc aaaactgttt gtcatttgtg tactaatggc ttcctctatg ttgtcaaggg
    16141 aagagtatga ggcataatta atgttgtcac agtttgcgat agctattata gttttgtatc
    16201 ctgtagaaca agttattacc aagttggatt cagcattgaa aaaataagac tttattaaat
    16261 tgcctgataa attgtaaata aaaatattaa cgtttcttat agaactatcg tttacatttg
    16321 acacttttga aatgatcgtt tctccttttt ttaataatga tatttttatc tcaacctctt
    16381 tttctgccgt ttggttttta tttaagttct ctattttatc cttataacaa cttacaataa
    16441 ttgtcgatat aagaattaat attgataatg ttgataatga atgaattgta tatttcattt
    16501 aaatagatcg tcattaagat tatatttgcg aattaaataa cttatttctg ggtcaagagt
    16561 acctctatat gcaattgata tatccatttg ttttgctttt aaaaaatatt taacagcttt
    16621 ctcttcctgg ttgtttcttg aagccagaat tgacataaga tatattgttt tggcattttc
    16681 aggcagtttg gcgagtattt tttcggcaat tgcattttga ccaagagaca tatatgcaat
    16741 tgcagtgttt acatcattat aagctgtaag gatttttacc gcatcctgat agttcctttc
    16801 ttttagaagt tttatcccat tcatatagtt tgaatctatt tctgaagtgt gaatggtatc
    16861 ttttatcata tttgtcttat gatgataaaa tgtaaaatca acctttctca gcaaaggata
    16921 aagttcttct ttgatatgat gataatcttt tttaaataac atcatagcca gctctctttt
    16981 gtcagggtca tcaattttaa agcattccat gatttttggt ctttctatta tgttactgtc
    17041 gttttgaatt agcaatgaga gttttgacca ttcctcaggg attgatctcg atattatttt
    17101 aacatctttt aaaatatcat tgaaatattt aaccactgat gcagctctat ttgctgaaag
    17161 tattttgttg gatttataac ttccttcagg tgatgccgtg gcggtaatga tcagagaatc
    17221 caacttaagt tcattactgt tgttattatc taaatattgt tttatcattt gtaattgatc
    17281 taaattgccg gataaattgg ggtcaagctt atactctcct gatttgaagg tgattgacaa
    17341 tgattttgtt gtgctaactt ttctaaatac aattttttta gtatattttt tagaagtatc
    17401 taaaaggctt accattgaag aaacattgta aatcagagtg tctgaaggct tgatgttaat
    17461 ggagaatcca ctgatgtcac ttatagtact gtaaagattt agataaagct ttgatgactg
    17521 ctcgttcgct aatatctctt gagtataatg aaaagcaaaa tctccattac tgcttttaat
    17581 cacagaatca agcttgcaat tcttaatttt ttggtttttt acatatttat tgaaatagag
    17641 atccttttct cttttacgct tttcgttttt ctcaataagc cagtttttaa cgtaatgatc
    17701 tattattttt ttttcactga ttccaaactc actttgaagt tcttcattaa aatgaccttc
    17761 aaagattctc gatttaggca gatttctttc aaggaaaatc attaaattcg gtaaatctgt
    17821 atagaatgtg taaaaatctg catcatccgg aatgattttg tgcaaatatt tttcatatct
    17881 acagtaactt ttttcttgag ctcttttaaa gtcctctcct tttaccacaa tcttgctaaa
    17941 atgtaatgtg tcgtctccac gacttaaaat tggcaataag actatttgcc atctgctgtt
    18001 cataagagac gcaggtattg ttacaacaaa tccgagagaa attgacccat tcctttcaga
    18061 tatattgtaa gaacgagagg aaattgtaat ttcatcaagc tgtttcacac ttatcatttc
    18121 accactcttt tcatctctta ctgcttcata gaggtaacct cctgcgtatg tgtcaatttc
    18181 atgggaaatt gcattattgc tgatttctct tctggataac tttacatcag aacttttttc
    18241 agatttgtca gcatgtgtta ctaatattgt attatgctct ctgtcaatgt tgtataattg
    18301 cttattacag gaagatatag caataatgaa aaaaagaatt gttatttttt tatatttcat
    18361 ctgttgattt tatagaatgt aaaccaagga aaatgaaagc tttgttggtc ttaacttcac
    18421 ctttttgaca tttcccaagt actctccaca attctttttt tcaaacctgt cataattaaa
    18481 aaacatagca cctcctgcac attcaaattc taaattaaat cttctgttta tcattttagc
    18541 atatccacaa ttgaaggaga ttccagcaca gtaaccctcc ttgcgttctt ttgaaaatgc
    18601 aaaattgaaa agggaggcta cgacacagct tccgacaaac atttcggagt agctatgcca
    18661 tagccatttt ctaaaccccg gctgaatgca caggtgttta atcttattgt ttttgcttaa
    18721 ttcaaatgga ttccaagaaa atggtaaatt gaaagtgatt ttgtcggaag tagcgatgga
    18781 tgcttcaaaa ttaatgcttc cgcatcctaa acctaaaata ttagtcccaa ttccatattt
    18841 ttgtgcctta catgtcatta aagtgataat aaacatgata ataaacacaa gaattttctt
    18901 taccatggtt cattaaaata aagattagca cacttttcct cattccatcc ttcaacagaa
    18961 gtttgtgaga aactcattga ttctgtgtca actattatgt taaaaacata aattactcct
    19021 tccaaaaatc cattatccat tgcagggata gatgtcccta ttttttttga tattatttta
    19081 ccgttaattt gcataaggac atctacaatt atcggaatat caatcttgcc ggataagggc
    19141 aacattatcc atttacaact attgttttca gctttgacag tgattatttc tgccgataga
    19201 caattagacg gaataatttt tccggttact aacgaaagtt tacctccttc taaacattga
    19261 ttaataaaga ttttggaaat aaacagagaa tcaatccctt taccagaata aaaatttaat
    19321 ataaaagctg tggctttatg tgcgaaatta aaagttatat ctgtgtctga ctttattttc
    19381 atttcttttt gggaagccca caaataatct ttgccattgg ttaatccttc atacattcca
    19441 tcttttaaaa tgagtgattc cataaaattt gaattagtgg aaatagcata gaaatcatag
    19501 ttccctatgg gcatcatgat gtttttatct tcatttacag taagataacc attgatgtct
    19561 gatttgacaa aaagtggcgt acccggatat gaagattttt ttgtagggtt atcacctgca
    19621 ttgtatgcaa gaattatcga ctcaataccc tcgggcatca aagaagaaga ctttgtgata
    19681 ccttcacgca tataactgcc cttgaaagat actttcataa gcttattctc acattgcctg
    19741 cagctatctt tttcttcttt tatacatgaa agtgagctaa atgtcccaat caggataata
    19801 gcgacagata ctaacttcat aatgaattta ttgttctgaa agggtaacac cacttatgtt
    19861 ctgtgttgtc caatcttcaa taagagttcc tgtgaactgc attgcagaag aggatattgt
    19921 gaaatcaacc atgtaataag aacctgacaa ataattttgt gcaggaatcg tagccgtata
    19981 ttttttacct gttactggag ttcctcctat tgttgcattt actattactt ctacgtttaa
    20041 atttactgca ttaacaggaa gcataataaa tttacccttg ttactgttta gagccatggc
    20101 agtcaacgac cccacgctgt ttgcactttc aatactgcca tctgaaagtt tcattttaga
    20161 aatggatgaa ggcgctgttg gagtgaaact aatagatgtt acgatcaaat tggatatccc
    20221 gatgcctgag gtaatattta tttctattcc aaccgccttg tgccaataga caaaagaaac
    20281 attaccgcca ttagcaatat ttgtacattt tgcccagaga taatctactt catttgtcaa
    20341 ttggcctgaa atacctgatg aaaaattaat ttccggtgct gttgaattat ttaaggaaac
    20401 ggaataaaaa tcatagtttc cttttggaag ataaatagga gatgcaggag tgagcaatcc
    20461 ggatgaatta cacgtagcat ctattggtgt acctgcgact gttggcgcag aagctgtgtt
    20521 tgcttcacta gaatatccta taattgtcac tttgttgcct gatgcaaaag ttgaagttgc
    20581 ttttgtttga gtatcagcac tgtaagatgc gtagaattta atctcttctt tttgtaattt
    20641 atttaaattc tctttttgac atgaagtaga aattagtaat aatataatta ttgagtaaaa
    20701 tattttaatg cttttcatga tttttatgat tttaaatgtt attagttgat atgtatttgt
    20761 ggatcataaa caagtgtttc ccaatccatt attgtcgtta ttttgtaaga tatgtcttga
    20821 ggagttatgt caaagtgaaa gtcgtaaact ttgccttgtt cgatatcaga tgagagaaaa
    20881 actcttgtat aaatagaact gacttttcgg gttgataatt ctttaaaagt gaatttgaag
    20941 tttaaatatt ttgatctgac tagtggaaca gaataataat agtaaatata aggttctgtt
    21001 tccaggtgac aactgtcgta gtattctgta acttcttgtg aataattgga cttaccagtg
    21061 atggtattta gaaaaacatt actgtcgtca ggagctgata tttcaacatt aataatttca
    21121 aatagatttt tagcctcttt atccatgtta caagcaattc taatcaagga atttgaaagt
    21181 ttgaatttca tatttaattg attttttggc gcttttttga ttttttcgga taaatcaata
    21241 gaaaggcatg catatccatt aaccattctt agcaaatcat cctttatgtt taaaatattg
    21301 tttgatttta tagctttaaa tgtgtaatat ccgggataaa gttttagtgt cttatcagag
    21361 taaagttctc tttcaccggc tcttattatg taattaactg ttactacatg agaacttttg
    21421 ttttgttcga agactttaat tataactcta tagtcttctc tttgaagatt tgtgtcatca
    21481 tcaataaata taacaggata ttttccgtaa tctggacaat tgtcgtaatc ttcctgtatg
    21541 catgatgata acataattag cagtattacg cctaatatgt aattgattct gtatgagtac
    21601 tccatgcttt tgtcttgcaa ttaactgaaa ttgtattgtt gttcttaact gttaaacaca
    21661 attcgtaggc attgccgcca ataaaatctt ctgacaatgt agcacgatat atccttcctg
    21721 tttggaaatc gccatctatc gatgcatcaa gaacaacctc aatgatcatg gacgcagtac
    21781 atggtaaaag ataaaaattc atactatgag cataaccatt aattgattca agtgcgccta
    21841 cattatttac aggtgctata taaccttctg aaagataaat cgatgctcca tcctgcgacg
    21901 ggaaagtgaa tttaacatcg gttacattga cagaagatac attaccttca ctcaatattg
    21961 tcaattttat ctgacaggaa acgtgtttaa aatgaaattc gacatttctg tcataagtta
    22021 tatttttctc ctttgaagct gcccacagat aatcaatacc atttacaact cctgatgctt
    22081 ggccgtcata gaaatataat tgtggagaat tacctgaatt ttcggatatg gaatagaagt
    22141 catagatacc atcagaaatt gataatgggt ataaagtaac aagttggccg tacccggaag
    22201 aaaaagcgga gactggggta tttccgacat aatttctcaa atcacctgtg tcaaaagcat
    22261 atattgttgc gtatttacca taaccaaaat attgacttga tttaacatca gggtaaatta
    22321 cagaacattt aaaattgatg ttgttcaatt gcttaacata aggcacatca atttgatgac
    22381 atccgataat tattaatatt gaaaatagta acgcccatgc ttttttcata tctgtctaat
    22441 tcacaagttt aatcagttca catgaattga gtagtggatt gtataatact gattcagtac
    22501 cagtttctga tattttaaat ggatggaaag ctgttatgtt aatgattggg aaaatgtcat
    22561 tgcaatttgt aatcatatgg tcaatccaag ctaactcatt gtctaatcct tttgtcacat
    22621 aggtttcgac attttttaaa taggtagtag ggtcattacc acaaattact tcaagatcat
    22681 aaatcacttt atatcctttg cttgatccta aacatctcac atatattctg tcagtgattt
    22741 catcggcaag ttcgcatacg tgttttcttc cgaattcatc atatgatatt ttattgtgca
    22801 ttttagcaac tcttttaata tgaaaataga cttgcacata cttgctatcc ggattaattt
    22861 cattttttgc agaatcagct tgagaagtct tcttctcaaa agataaactt tgttccatct
    22921 tagtacagga gagagccaga ataattaatg ttaaaattac tattgtcttt attttcatag
    22981 aatgatatta tattaagtta attaagagta ttgaacagaa tcatgtttgt tcggaggccg
    23041 ttcgtgcagg aaatgacgaa gttttatgcc tattggcctt agtttgttaa caaagaccaa
    23101 tattatgtcc aatataattt tcccctccat ctctcgcctc attttgtgtc aaaagtaaga
    23161 gagaaggggt ttaatattgc ttataaaaag ataaaaaaaa gaaagaaaaa gataaatgtc
    23221 aatttatcta tatcaataat aatcaaattg atatttatag aaaaaaataa ttttaacaga
    23281 tattagtatg agcgatcgtc acacccttcc atccagttga tgaatgctgt atttacaact
    23341 ttattcccac caggagtagg gtagtctcct gaaaaatacc aatctccact gttttctcta
    23401 caagctttat gaagattttc tatactttgg aaaacaattt gaacatctgc cttgatttca
    23461 ttgcctttga ctagttctgc aatcttgtcg gcaatttgtt catcagtgaa aggttcgtag
    23521 attttcttaa cgtaattgat atatttttct ttaggcaaac caacttgatt tttgcacttc
    23581 atatatacat caaggattat gtcatccaag ccatgttcct tcaggagggc tatagctgca
    23641 ttaaaggcta taaactctcc cattcttgtc atgtctatac cataacagtc aggatatctg
    23701 atttgaggcg cagaagatac aataattatt tttttaggat tcagagtgtc gagaatttta
    23761 agaatacttt gttttaaagt tgtaccacga actatggagt catcaattac cacaagatta
    23821 tcagtctctt ttactgcacc atatgtcacg tcataaacat gttctaccat tacgtctcta
    23881 tctccatcgg atgttataaa tgtccttaat ttaacatctt taattacaat tttttccgtt
    23941 ctgggctcct ttaaaataat attttccaaa gatttttcgt caattttatc accattatga
    24001 ataatgttaa tgatttgagt agttttgcga ccaactaaat atttctgaat accactaatc
    24061 attccgtaat aagaactctc agcagtattg ggaataaaag agaaaacagt attgtcaata
    24121 tcttcgtcga ttgctttaat tacttgtttt gataaaagtt tcccaagcat ttttctctct
    24181 ttatatattt gactgtcatt gcctcttgaa aaataaattc tttcaaaaga acagctactc
    24241 cttttcaaag gttctcggat taaggtttca ctgatttctc cactattttt aataattaat
    24301 gcataacctg gttttatttc attaatcatt gaatcattta ccttgaaaga tgttcttata
    24361 acagatgctt cagaagcaac agcaacaaat tcttcattaa cagtataata taccggtcta
    24421 atcccccatg gatctctgaa aacaaatgca tcaccatgac cgagtacacc tgcaactgca
    24481 tagcctccat cccaattctc tgtactttta cttaagacat ctgcaagatt caagtttttt
    24541 tcaattaaag gtgttatttc gattttacta taaccttcat cacgatattt tctatattta
    24601 ttttgtatct catcatcaag aaaatatcca actttttcaa gaagagtcac agtatcggca
    24661 taaaagatag gatgttgtcc tgcccggatc aggctttcga aaagttcgtc tatgtttgtt
    24721 agattgaagt ttgcagcgat ggcaagattc cggcttctcc aattgcttgc cctcttaaga
    24781 ggatggacaa gatcaatatt atttttaccg aatgtggaat atctcaagtg ccctaaatag
    24841 atttcagaaa taaacggtaa attcatcttt gcccattgtg catctctttg ttttaaattt
    24901 tcagttacaa atggctcgtt tattttattg aaaacttctt gaatcggatt aggatcacag
    24961 gaacgggctc tgtccatgta tttatatccc ggaggcatat cgagtttaac tccaaccaca
    25021 cctgctcctt cctgacctct gttacgttgc ttttgcatca taatataaag tctgtttaat
    25081 ccgtaaaggt atgagccata tttgttttga taatactcta gtggtttaag taatcttatc
    25141 aatgcgattc cgcactcgtg attaatgatt tcactcatga ttatttggtt ataggtctaa
    25201 taacgattcc tctactgttc ttgccatcaa tgcatacctt taatggtact ttaaaacgta
    25261 tgtgtcttaa atagttgctt tcatgaactg cttcaagaga atctaaatat tctctgtcaa
    25321 attttccgtc atccagaaag ggattaattg ttaaatagcc aataccaagt gatgttaaat
    25381 tttgaaaaaa atgtgtgccc tgacttggtt cgacatgaaa atttttctgt ccacattcga
    25441 caattacttt agcctctgat atgtctgacc atttgaccgg aattccaagc caggaatcac
    25501 ttgagcccca tctgccaggt ccaattagaa tataattatt tccttcttct tttaaatttt
    25561 tgttaagata atttatctcc gcagcaatat cttttgtacg ggaattgtca aaattttctt
    25621 ctcttatata aataatatct gtaattcctt ctatattacc aactccaagt gccttttcag
    25681 cataaattat acagtcattc ttatctattt tgtcccagtt aattgatata tagtcttgat
    25741 tatcggctac tggccttatt tgaagaagat taaaaattat gtcttggccg gcatcgacat
    25801 ccatgtttac agcaaattca atttctacag gtgtacgcat ctctgtttga ctaatattca
    25861 atatatcaca aattatatct gccaatggga atacatcatg ttttaaaatg tgagcaaatg
    25921 ttataacttt ccttccttca acaagattac tgtcaattaa catctcatct tgtgcattcc
    25981 atgtagaagc tacatgttgc atgttcctga aatgttttat ctgatttata tcaaatttgg
    26041 ctaaattaac agattcgtct atggatgttt tgaactcttc aggtttgaga ttcagcgcat
    26101 acatatgatt ttgagtctct tttaatgcaa gttcaggggt cgaaagttga agaatatgtt
    26161 ttgggtgctt tggcgaaaat ctaagacatt tctctccttc aactacgagt ctcccaagtc
    26221 cgaaggccat attgacaatc ccgtcttccg gtttctcgtc tccaatagga taaaaattaa
    26281 cagacctggc aactcctgat aaagttggaa aaaagtatcc actgtcctca gttccacata
    26341 catcctgaat aacaactgcc attttctctt cgctgataag attggaagtt gcttggatat
    26401 atgaacggct tgatgcataa tatactgatg cataaacact ttttatagct ttgcccaata
    26461 gacgcagcat ttggtcactg ttttgagtga aaggaatcat gtaagtggaa tatattcccg
    26521 cgaatggttg ataatgggag tcttcaagtt tggagcttga tctaacagcc aaagggccat
    26581 taacatattt aatatattca cttaagtctt caattaatct tgcaggaagc ctcgaactgg
    26641 taaattctga taaaagttct tcatctgaaa tctcggaatt aattacatat tgcaggccat
    26701 tttctttgat aaattcatca aagtaatcgg ttgctatcac tatagttctt ggaataagta
    26761 ttttaactcc agggtatttg ttcaacagat tatgcttttg aagcatgctg ttaataaatg
    26821 ccagtcctct tgcctttccg ccaagagatc cttctccaag ccgggcaaac caaataaatt
    26881 cttcatatgt ttccttattg aacttagcta caactccatg gcctactacc attctatatt
    26941 caataatgga tttaacgata aagtttctaa gttcattgat actgttgaat tgtccttctc
    27001 tgacatccct gattttattt gccagatcaa acagacccct tgaatacatc cactttgaaa
    27061 gtcgatttct tgaagcgtga tagagcaata catcttcagg gatttccatt attaagtgct
    27121 gcaaatcttt aagatctttt gcttttccgt atacaatacc gctattattg tctctgaaaa
    27181 caaaatctcc aaaaacgaat tcttcactaa tgtattcact caattcgagc aagagagtct
    27241 ttgaatgttt aagaataaaa cctactccca tactttcagc cacatttctc atactttctt
    27301 gtgaggattg cagaagaaac ggcatataaa aatcatcatt tttgatcatt ttgcaaagat
    27361 caatcccggc atcaattttc tcactttcag gaggatcgtt cttgtgaatg acaaatgcta
    27421 catcagaaat tactccaaga aggttttgtt tatatttctg atatagtgag acggcttctt
    27481 cgtaacaagt agcaagcaaa attttgggtc ttgctctttt cctgagtttt tgctgttgtt
    27541 cgttaagagt ctcctttaga aattctgcac tttgctcaag tacaagtcta taaattgttg
    27601 gcaaataagt ggagtaaaac cttattgaat cttcaaccag aagaattgat tgtacgcctg
    27661 cgttcaatat gtcatcatca gcattaattc tgtcttcaaa cagcttgatg attgccaata
    27721 tgaggtctgc attgccatgc cagctaaata tatggtcaat tgcagaacaa tcttcttctc
    27781 taacaatcct ttcaagttct ctggagaaat ttgaaagcaa aacaaatgga atataaggat
    27841 gttcaacctt gcatagcttt gaaaatttaa agacatcaag ctctcccaca tttatcatac
    27901 tgatgattag gtcaaaatca tcgtcagaat cgagtaactt caatgcatcc attgaagaat
    27961 tgacccatgt aaaagtaggg ggattgctca agttaagatc tacatactct ttatagatct
    28021 gatcttcaat ccgcccatct tcttcaagaa tatatgcatc ataacttgta cagatcatca
    28081 gaactcttct tatgcgattc tgcattagaa tcttataatt catctttgaa cttatgttct
    28141 gactctcttt ttcagacata taataaatgt agtttattgt tgttttctgt aatcgtaaaa
    28201 tccaacacct gtttttctgc ctaattgccc agatcttacc atttttttca aaagaggatg
    28261 tggcctatac ttagggtctc caaattcttt gtatagtacc tccatgatag ccaggcaaac
    28321 atcgagtcct atcagatccc caagtgcaag aggccccata gggtgattgg cgccaagttt
    28381 catggcttca tcaatttcat ctcttgttgc aacgccatca gcaaggattg atatgccttc
    28441 atttacaaga ggaataagaa ttctatttac cacaaaacct ggagcttcat tgacattgac
    28501 aggagtttta ccggcttttg ttgctatatc tgcgacaaat tgctgggttt ctacagaagt
    28561 aagttgtcct ttaatgattt ctacgagttt cattacgggt gcgggattga aaaaatgcat
    28621 acctattact cgatctgctc tttttgtaga tgctgctatt tcagtaattg aaagagaaga
    28681 tgtattagat gcaagaacag ttttaggatc acaaatttca tccaatgttt taaaaatttc
    28741 atttttgacc tccattgtct caaaaacggc ttctactatt aaatctgcat ccttgcaaat
    28801 atcataagat gtagtgccga ctattcttga aataattgat tcaacttcac ttttatcaat
    28861 tttacctttt tctgcaagtc ttgagagatt gctattaatt tttttgaatc ctgattgaag
    28921 ttcggcatca ttcagtcctt tcattatgac attgaagcca gattgagaaa aagcttgaac
    28981 tattccgctt cccattgttc cacttcctat aacgcaaatt ttcatagtta attataatta
    29041 aattatcgat tattaaattt aactttagtt ttgtttaaaa aggccttcat gccctctttt
    29101 tgatcttctg taccaaaaca cgcagcaaaa tactgtattt cattttttat ggcctcatca
    29161 actgtgagat cagctgaaat attaattaga ttttttgcat tacggactgc aataggggca
    29221 ttttttgcaa tatcattggc taatttaatt gcattttcca taagagtctc ctgtgggaca
    29281 acactattta caagacctat ggataaagcc tcagtagaat taataatttt tcctgtgaat
    29341 attaactctt ttgccttggc tattccgaca atacgggtca tcctgaaatt tccagaaaag
    29401 cccggagtaa tacccagtcc aacttcaggc tgtccaaact ttgcattttc tgacgcaatt
    29461 cttatgtcac atgccatggc caactcgcat cctcctccaa gtgcaaatcc attgacggca
    29521 gctattacgg gaacaggaag caattctatt tttctaaaaa ccattgaacc aaaatatcca
    29581 aattcagctg cttcaattcc atccattgaa accatttctg aaatatcggc tcctgagacg
    29641 aatgattttc cttcgccagt aataatcaat acccttatat cttcgttttt agaaagattg
    29701 tctattgcat catcaagttc ttttaaaata taagaattta gtgcattaag ttgagaagga
    29761 ttactgattg tgagggtata tatctgatta tcaatggata tatttagata tttgtatgtc
    29821 ataatttgta attattttta taatttatgc aaaaataaga taattccatt tacatattaa
    29881 taatttttct atattcgcag ccgttatgta cgcctttaat tttttacata ttcattattc
    29941 tctaattctc agcctatgct ttggtaatga ggtggagagt tgatgtattt cagaaagata
    30001 acttgaacga tatatgaacc agccacctca atatgaggtg gcttttaatt tttatgcagt
    30061 catgaaagag aaactttatg tatttgacac aactttgagg gatggagagc aagtccctgg
    30121 ttgccagctt aacacaattg aaaagataga acttgcgaaa gctctggaac ttctaaaggt
    30181 agacattatt gaatgtggtt ttccaatctc aagtccagga gattttacgt cagttgttga
    30241 gatatcaaaa gtcgtaaccg aatcggtaat ttgtgcactt acgagagctg ttgacaaaga
    30301 tatcgaggtt gctgctgatt cacttcattt tgcaaaacgc aagagaattc atacagggat
    30361 cggaacttct gatccacata tcctttataa gtttaagtca aatcgggacg agatacttga
    30421 aagagcagta cattgtgtta attatgcaag aaaattcact gatgacgttg aattttatgc
    30481 tgaagatgca ggaagaacag acaatgatta ccttgcaaga gtagttgagg cagttataaa
    30541 agccggtgca acagtagtta atattcctga cacaacaggc tactgtactc ctgaagaata
    30601 tggcaagaag atactctatc taaaaaacaa tgttcctaat attgataaag caataatatc
    30661 tacacattgt cataatgatc ttggcatggc aacagcaaat acccttgcag gagtgatgaa
    30721 tggtgcaaga caggtagaag tgactatcaa tggagttgga gagagagcag gtaatactgc
    30781 tcttgaggaa gttgtaatgg ctattaaatg cagaaaagat ttcccggtag aaacaagtat
    30841 agatacgcat ctaatcatca aaacatccag gttggtttct actttaatga gaatgcctgt
    30901 gcagccaaac aaggctattg tcggaagaaa tgcttttgct cactcttcag gaatacatca
    30961 ggacggagtt ttaaaaaaca gagaaagtta tgaaataata gaccctaatg atgttggtat
    31021 aaatgaatca ttaatacctc ttacagcgag aagtggtaga gcggcattga aacatcactt
    31081 tttacgtttg aatattgatc ttacaagtga agaattggat gtggcttatg aaaaattttt
    31141 gagactagcc gatgtcaaaa aagatattca tgatgaggat ttattaatga tttcaggggt
    31201 cacaaaaaat attcagactc acaaaattaa acttaaatat ctccaggttg tttgtggtaa
    31261 atctgctatt ccaatggctt ctgtcaagtt gcttattgac ggtgaggagt gtacagccac
    31321 aagttctggt aacggtccta ttgatgcggc cttttcagca gttaaatctt tgataaacag
    31381 gaagatttat cttgaggagt ttcttattca ggcaataact agaggaactg atgatgttgg
    31441 aaaggttcat attcagattg aaaataaggg aaatatctat tatggatttt ctgcaaatac
    31501 tgatataatt acagcttcag tagaggcact tattgatgca gtgtcaaaaa ttatataaaa
    31561 aagtgcaaat aaattacaaa tgaaaaagac gctatttgat aaaatttggg aatcccatat
    31621 tgtaaaagaa attactggag gcctttctat tttatatatt gacagacatt acatacatga
    31681 agtaacttct cctcaggctt tttctgacct ggatagaagg ggcttaaagg tttttagacc
    31741 atataagaca tttgctacat gtgaccataa tgttccaact ttaaatcagc atctgcctgt
    31801 acaagactct tcatcaagaa tacccattga aagacttgcc gaaaacacgt ccagacacgg
    31861 tattttatat tttcctcttc aagacagaca taacggtgta gtgcatatta taggccctga
    31921 acttgggtta actacttgcg gaatgacaat tgtctgtggt gatagtcata cttcaacaca
    31981 tggagccttt ggcaacattg catttggtat aggaaccagt gaagttgaaa tggttcttgc
    32041 cacacagtca attcttcagt ttaaacccaa acagatgttg ataaatgtta acggaaaatt
    32101 aactaagggg gtttgtgcaa aagatttaat tctgcatatt atttcaaaat taggtacatc
    32161 tgcaggaaca ggctatttta tagaattttc cggtgataca atatcttcgt tatctatgga
    32221 ggagagaatg acgatttgca acatgagtat cgaaatgggt gcaagaggag ctcttattgc
    32281 tcctgatgat ataacattca gatatttgga aggcaaagag tttgctccaa aaaatgaggc
    32341 ttggatagaa tcggttaata tgtggaagtt gttgaaaagt gatgaaggtg cagaatttga
    32401 tattgtttac aatttcaatg ctgaagaggt tgaaccaatg ataacttatg gaacgaatcc
    32461 tggaatggga ataaatgtaa acggagttat accacaaaat gcaaaagatg gagatagctt
    32521 tagtaaagct ttggcttata tggggtttgt tagaggagaa aaaatcattg gtaaaaagat
    32581 tgattatgtg tttcttggaa gttgtactaa tggaagagtt gaagatttca gagcatttgc
    32641 ctcaattgta aaaggaagaa aaaaacatcc agacattatt gcttggttgg ttcctggttc
    32701 taatggagtt ctgagagcga tagaagagga ggggatagga gatattctaa gagaagccgg
    32761 ttttgagata agagaaccag gatgttcggc atgccttgcc atgaatgaag ataaagtgcc
    32821 ttcaggtaaa tattgtgttt ctacctcaaa cagaaatttt gaagggaggc agggaaccgg
    32881 tgctagaaca ctccttgccg gacctttggt agctgcagct actgcaatta acggagttat
    32941 aacagatcaa agaatttatt taatatcatg agctttgaaa aatttataga acttatttca
    33001 gaggccataa ttttgcctgt tgataacatt gatacagatc agattatacc tgccagattt
    33061 cttaaaacaa ctactcgaga aaattttggc aaaaatcttt tttatgactg gcggtatgat
    33121 caggatggtg agcctataga atcatttatt cttaacaatt ctgataataa aggcagaatt
    33181 ttggttgcag gcaataattt tgggtgtggt tcaagcaggg aacatgctgc ttgggcatta
    33241 tatgattatg gtttcagggc agtaatttca actttttttg cagatatatt caagaataat
    33301 gctctcaata atggcttatt gccaatacaa gtttctcgag ctttttttca aaaaatagtt
    33361 gataattctc attctttgct tgatataaat ttaccgatgc agagtgtcac aataatctcc
    33421 acaggtgaca gtgaattatt tgatataaat acctataaaa aagattgctt aataaatggt
    33481 tttgatgata ttgacttttt actgtcaaat attggtgaaa ttgaaaaatt tgaacaaata
    33541 aaaagtacaa gcaggttatg aaaaaaaaaa tagcagtttt gcctggtgat ggggtaggac
    33601 ctgaaataat tgaacagagt ataaaagttt taactgcagt tgagaagcgt tttggtcatt
    33661 cttttgaatt tgcatatggt tcaattggag catgtgcaat tgatgaattt ggagacccat
    33721 accccgaaaa cactcataaa ttatgtcttg aatctgacgc tattttattt ggagcgattg
    33781 gtgatccaaa atatgacaat gatccatcag ccaaagtgcg tccagaacag ggtttattaa
    33841 aaatgaggaa ggatttgggt ttatatgcaa atattcgtcc agtgattata tatagtgcat
    33901 tacttgacag gtctccatta cggacggaca ttattaaaaa tgttgattta gtggttgtta
    33961 gagaactgac tggaggtatt tactttggag aaccaagggg gaggagtgaa gatggccgaa
    34021 aagcctttga tacatctatt tactctgatt ttgaaattga gagaatatca agaattgcat
    34081 ttaatttggc cagaaaaagg aaggggcagg taactcttgt ggataaggct aatgtattgg
    34141 ctacctcaag attatggaga gaggtggtga gtagattgca taatgaagaa tttaaggaaa
    34201 tcaaactcga ctttcttttt gttgataatg cttccatgca gttggtcctt aatccttcta
    34261 agtttgacgt cattttgacg gagaacatgt ttggcgatat tcttagtgat caggcaagta
    34321 taatacctgg gtcaattggg cttttgcctt catcatccat gggtgtttat actcctcttt
    34381 ttgagccaat tcatggttct tttccccagg gtgcaggaaa aaatattgca aatcccatcg
    34441 ctacaattct ttcggccgca atgatgcttg atgatgcttt taatatggta tatgaggcat
    34501 tatctgtaag aaaaggatgt gaagctgcaa ttaacaatgg caagcttaca attgacttaa
    34561 cagattctaa agaagcctgt actacatctc agataggaga ctatgttgca gactttatta
    34621 tggaagcacc ataatggcgt ctattattaa atagatacct ccggaaatca acaatgtggc
    34681 aggaattgta aaaatccatg ctcctactat atttttagca gttgcccatc ttacagaagc
    34741 aattcctttt gtcatgccaa caccgattat cgatcctgta attgtatgtg tggtacttac
    34801 aggtattccg gagagagctg tgccaattaa agtgatagct cctgctgttt ctgcacagaa
    34861 accttgtacc ggttttagct ttgccagacc atccccaaga gttttgataa ctctccagcc
    34921 tccggttatt gtgcccagtg ctataactga ataacaggtt atagttatcc ataaaggaat
    34981 atgaaatcca gcatttggtt catatagata tttagcaata aaatcattgg ttcccgcaac
    35041 gctgaaaagt aggactgcaa ttattcccat tgtcttttga gcatcattgc ttccatgtcc
    35101 aagactgaat actgcagaag aaaatagctg taaaaccttg aagttctttt cgaccttcat
    35161 aggatgtttt ttcttgtgaa tccacattgt tagggccatc attaagaatc ccagaaaagc
    35221 accaataagt ggagaaagga ctataaatat ccctactttt agaattcctg tccctattag
    35281 cgcactagaa ccatgtgtaa accaaacagg accaatcaaa cctccaatta acgcatgtga
    35341 aacactgatc ggcattccca gtctggtaca agcataaacc catataactg aacctgtcag
    35401 tgcagacaga atgataaagg ggttaactac atttggatcg actatccctt taccaatagt
    35461 atttgctacg tgtactccaa atagaaagaa agccgcaaaa ttccaaaagg cagcccataa
    35521 aacg
//
LOCUS       DAOTQL010000002        34432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_1376_length_34432_cov_5.619688, whole genome shotgun sequence.
ACCESSION   DAOTQL010000002 DAOTQL010000000
VERSION     DAOTQL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34432
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1376_length_34432_cov_5.619688"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..78)
                     /locus_tag="P5293_00130"
     tRNA            complement(5..78)
                     /locus_tag="P5293_00130"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Pro,seq:tgg)
     gene            302..838
                     /locus_tag="P5293_00135"
     CDS             302..838
                     /locus_tag="P5293_00135"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRR48374.1"
                     /translation="MKNHFFTTSHESLIRGIIALIIGGLAVFMPDITLRSFVICIGIL
                     ILVSGIVSFVFSLRSQVRVTKNIFTANALFNLIFGLLFVLLPTMMVNIFVIAIGIGFL
                     LIGIFQLISALSIRHDYGWSWIYFSISMLMIIGGIVLFTNPFTSAQTILIFIGIFSLI
                     YGVTELYMAWKLNKKLNH"
     gene            complement(916..3648)
                     /locus_tag="P5293_00140"
     CDS             complement(916..3648)
                     /locus_tag="P5293_00140"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR48375.1"
                     /translation="MIKKFGLLTAIILLSFSSFSQPRGSLRADITLSVRDSLTDAPLE
                     LVTAVLSKIDKNHKKEIFTYAISDSSGNIKFNRIPASDYEISLQYMGYFMKIVPVKID
                     VKELIEGNNKFDLGVIKLRENVVELNAVTIREHVAPIKYLGDTIQYNAAAFKLTDTDV
                     LEDFFKKLPGWSVDRDGKITANGKVIEQITVNGRIFFLNDPVFVSRNLPAKLLKNIKL
                     FEKQSERSTFTGIDDGARTNTVDVTIKEDMLNGWVGSIKAGGGTDKRYKTNDFIANFN
                     KYNQIAIVGNASNLNEAPMMPGVAVSSGLSRNYSIGSNINLSNKKNNFEADISYKLND
                     KSIFNDNEVHRTNFLKDSIFVYDKSSRSDNSNINHLLSGQINWSGKKSKISINPKVMM
                     VYGNYSDSVKYRTIGGFSGVTINEGESVNSGRRSIENFSTDIQYITKTGKSMRTFSIK
                     GTVGIDKRESEGKNKILNRSDQQYFTDDKSFDVSAKVSYTEPITKKIIFGSNYSIASS
                     IYSQSKETYNSDNNGNYSELDSLLSANSNNIELTQNIEFYLQKPKRKDEVSYFMLGAS
                     ILPSYLKRDSHQQEFDKWFWNISPNAEYRLTTKDFLSIFLKYSGNVRTPSLTLMMPIP
                     DNTNPLLIRLGNSELKSEYEHNVNLSIRKITSLSGGFGYGILLTTKASYFKNRILNKS
                     WFDEDGIQYTMPINYSGDYFITSNLHWEQPFFKGVLTIANIINGGLYNNISFVNGEKN
                     TTKRNVLGESLNLTLKLDKIYFQTKATFNYELSVNSIFPEKKTKTWRNVIEGYISYSM
                     PFDITFKSDIAYQYFKGYTAQYDKPYLLWNAAISRPLIKNKLTISFSANDILNQNKNI
                     QREVTDFYIQETRYNTVRQYFLFSLTYKFLIGGKTGAFKERANSVIRSNETITY"
     gene            complement(3800..3907)
                     /locus_tag="P5293_00145"
     CDS             complement(3800..3907)
                     /locus_tag="P5293_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR48376.1"
                     /translation="MTAALVDRLTHKAYRIDKNGKSYRLKETRQMIGEK"
     gene            3976..4221
                     /locus_tag="P5293_00150"
     CDS             3976..4221
                     /locus_tag="P5293_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48377.1"
                     /translation="MSVENLCDRFGYSRQAYYKSKVVQERYIVDEAVIISAVEEIRSE
                     KDFPRLGGQKLLSMLIGLGFDIGRDSLFDILRSNKLL"
     gene            complement(4522..4923)
                     /locus_tag="P5293_00155"
     CDS             complement(4522..4923)
                     /locus_tag="P5293_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRR48378.1"
                     /translation="MSFRYPVYRLQIYKHPIWKGFVKRMGYAFQAEGIKIGFYYSLLD
                     WHHPDYTIDRNHPRSQTSDSAYAAVNKGKNMDVYREYVKNQVTELLTNYREVSIIWLD
                     YSFTAPFNMTSGLSSKEKLVDTFIPLETSKE"
     gene            complement(5101..6456)
                     /locus_tag="P5293_00160"
     CDS             complement(5101..6456)
                     /locus_tag="P5293_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR48379.1"
                     /translation="MKFVDRIEESKRLKQVLTANKSTFTVIYGRRRLGKSTLITRTLG
                     ANDVYYLADQTDATLQRKLLAKMVASIIPGFDKAIYPDWNVFFDTLNDRTSQKFTLCI
                     DEFPNLVESSQELPSILQNKIDSKRLKFNLIICGSSQQRMYGLVLDATSPLYGRADAI
                     LKITPIKVPYIQDALGVPAVEAVEEYAVWGGVPRYWELREGYANLREAIENNLLSVNG
                     TLYEEPLKLFKDDINDIVKTATIMSYVGAGAHRMSEIAGRSGEIATNLSRPLAKMISL
                     GYLTKEIPFGENEKNSKKSLYKISDPFMNFYFRFVIPNRSFIEIGRISPILADLDAHF
                     SDFVSEYWEKLCRETVSGNELDGITYGIARRWWGNVSREERIELDVVAESSDKKYLLV
                     GECKWTTQENSGRLLAELKEKAQKLPFAKNHTIIPKLFLKNIPEGTLTDVLLPEDVMK
                     K"
     gene            complement(7081..7167)
                     /locus_tag="P5293_00165"
     tRNA            complement(7081..7167)
                     /locus_tag="P5293_00165"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7131..7133),aa:Ser,seq:gct)
     gene            complement(7233..9095)
                     /locus_tag="P5293_00170"
     CDS             complement(7233..9095)
                     /locus_tag="P5293_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48380.1"
                     /translation="MNRSICVLLLFLLAGGLSFGQTPDLKSKFISAEEHRKNYRFEEA
                     IAIYQQLLEISKDEEFNRKVESRIANCENGLKMLDYAARPRVLGAANVPVKDFFLYYP
                     DIPDSSWILVPYTLNKNMRSYPINNVMRLSSNKDIIYFSAQNQEGKWDIFSSQCIDNE
                     KWSSPEPVNTLNSQEDEILPYQSDDGKKLYFSSNGLAGMGGFDIYVSYWDEKRNVWDT
                     PQNLGFPYSSPADDYLYVESDGGDYAIFASNRNCASTDSITIYILSFEANPVRSPVQS
                     IEEAAQIASLNTGSYLKANNYGTPADTLKKDTETNEYTRMIKEARQLQKEIDENIRAI
                     NANRALLATLNNEDDKNYLIKKIGEDEMILLEKQSKLMIANKAIQQKEIDFLNKGVII
                     PREEGMWDKPVIINNQAEPFKLKLSHYGKLPYMEIVEPVKPVDLSFRTSGNFVLIENE
                     QDIPEELFYTIQLFMLSAKADSASFKGISPIFETKTQNGKFNYTAGRFYSYKELSDAL
                     QKLKSMGFRDVLPLAYYKGKTISIKNARLLEDKINSSATYQVKITAFPDGIPQYVLEL
                     IRNNTEKDIAMKSVAGKSVYFIGPFNSKSEADILLSILKGVAGEEINVEAIEKK"
     gene            9212..12307
                     /gene="secDF"
                     /locus_tag="P5293_00175"
     CDS             9212..12307
                     /gene="secDF"
                     /locus_tag="P5293_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRR48381.1"
                     /translation="MQSKGAIKLLAIVIALACIYQLSFTLAVKHQEKKAVQYAERAVK
                     IEQTLPSFQNVPELDRAYYLDSLRNLKEKFYIDSISAEKVYLGFTYKEIKEREVSLGL
                     DLKGGMNVMLEVSVADLVNSLANNNTSPQFIEAMRVARENQANSKSDFITLFAEAWDK
                     VAPGQRLSQIFGTYEMRDKIKPESTNDEVIAVIRKEAESAISNSFNVLRNRIDRFGVT
                     QPNIQKIGNTGRILVELPGVKEPERVRKLLQGTASLEFWETYENPEVYKYLAEANNVI
                     KDYLASGITKDSLSSTDTLAASAKSVSDSSKSAEADSLLSQIRATDSLSVDDKTLAKD
                     NPLFFLLNPNVYQGQLMEGPCVGRAHYKDTAKIGAWLRLPQVQAVFPSDLKLLWTVKP
                     IDKAGTIYELIAIKANTRDGKAPLDGSVITDASKSFGSTRGTPEVDMGMNAEGARTWA
                     TMTANNIGKCIAIVLDGMVYSYPRVNSEISGGRSQITGNFTVQEADDLVNVLKSGKLP
                     APARIVQEAVVGPTLGTESINAGMLSFLIAFLLVLIYMIFFYNGAGVAACVSLIANVL
                     FLLGTLVSFGAVLTLPGIAGIVLTMGMAVDANVIIYERIKEELRAGKGLRLAVSDGFS
                     NAYSAIIDGQLTTIITGIVLMIFGTGPVQGFATTLVIGIITSLATSIFIPRLIFEGRL
                     AKNKSISFSNKLTQNFMSNTKINFMKVRKIFYAISISLIVISVGSMVFKGFSYGVDFT
                     GGRTYVVRFDQNVTTEEVRKAVMDVFNEGVEVKQFGGESQVKITTKYKIEDNSSETDA
                     LVDQMLYNALKGFFATSITFEEFTATTVNPNGIISSEKVGPTIADDMQRNAIIAVFIA
                     LLAIFVYIAVRFRKWTWGTGGVLALFHDTIIVIGFFSLFSGILPFSLDIDQTFIAAIL
                     TIIGYSINDTVIIFDRIREYRRLYPKRDLAININEALNSTLARTINTAGTTLVVLIAI
                     ALFGGEVIRGFAVALILGVAIGTYSSVFIATPIVYDITGWLNKKKEEKKLVVNTGKNK
                     K"
     gene            12415..13791
                     /locus_tag="P5293_00180"
     CDS             12415..13791
                     /locus_tag="P5293_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRR48382.1"
                     /translation="MELPFAESYKIKMVESIRKSTRSQREQWIKDAHFNLFNLKSDHV
                     FIDLLTDSGTGAMSDKQWSAIMEGDESYAGARSYYKLKEAIEDITGFPYFLPTHQGRA
                     AENVLFSSIIKAGDILPGNSHFDTTKGHIEFRKAHAVDCTIDAAADTTLELPFKGNMD
                     LDKLENVLKKYPKEQIPCIVITVTNNTAGGQPVSMENIKGVSDLAKRYGVKVLIDSAR
                     FAENAYFIKTREKGYENKSIREIVREMFSYADFMTMSSKKDAIVNIGGFIGFRHEKDW
                     ISCQMYNIMFEGYITYGGMSGRDMNALAVGLYEGTEFDYLETRISQVAYLGHKLDEYG
                     IPYQRPSGGHAIFLDAKKILTHVPKEQFIAQTLGVELYLESGVRAVEIGTLLADRDPD
                     TRENRYPELELLRLAIPRRVYTNNHMDYIAAAVKNVFDRRESINKGYIITKEMPIMRH
                     FTIELKPA"
     gene            13907..14095
                     /locus_tag="P5293_00185"
     CDS             13907..14095
                     /locus_tag="P5293_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48383.1"
                     /translation="MQNKNVINPVSLFFTNIDMLIEKNIVAIRNGIITWSSVHAEPIW
                     GRLNHLVTSTRIYAVITM"
     gene            complement(14170..14739)
                     /locus_tag="P5293_00190"
     CDS             complement(14170..14739)
                     /locus_tag="P5293_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRR48384.1"
                     /translation="MYIEFHPLEPFLPPEAKILMLGSFPPPRNKWSMEFFYPNFQNDM
                     WRIFGIVFHEEKEYFITNKKFDAVKIKDFCYKNGIAIYDTATAVKRLRGNASDNYLEI
                     VDMLNLKELLLKIPKCNTIAATGKKALDIILKTANCKEPEIGNFSFFQLENRQIKLYR
                     LPSSSRAYPMPLNIKAAYYQRLFSSINML"
     gene            complement(14747..16732)
                     /gene="rnr"
                     /locus_tag="P5293_00195"
     CDS             complement(14747..16732)
                     /gene="rnr"
                     /locus_tag="P5293_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRR48385.1"
                     /translation="MSKNRNAGKTKKFKEKNKNTKRSPREEVIGRVSMTREGYGFIIR
                     DGFDDDIFVSARKMRGALNGDTVKVALTAKKTKTRNMEGEIIEIIERSKKPFIGVLQI
                     TGSQAWVITESRNMPYDIRIPIENVKDTQNGLKVAAVVDEWPRKSDEPIGHIIDVLGT
                     PGENNTEMHAILAEFGLPYKFEVQVEKEADKIPENISLEEISSRKDFRNVPTFTIDPA
                     DAKDFDDALSIRKLEDGNWEIGVHIADVTYYVKPGSLIEKEAQERATSVYLVDRTVPM
                     LPEKLSNKLCSLRPNEDKLCFSTVFVMNDNAKIIDRWFGRSIINSDYRFNYDEAQNII
                     EGSEGPMKEEILKLHKLATILRKERFSKGAISFERPEMKVEIDENGKPIGVYSKITKE
                     SNWLIEEFMLLANREVAYLIGKKGKNAKTFVYRIHEEPDVDKVAAFRTFIHHFGYEMK
                     PTKNSREISSEINKLLETAKGKPEEEAIEIMALRSMARAKYSTDNVGHYGLGFDYYTH
                     FTSPIRRYPDMMVHRLLSMYLDNAKSQEKKYYEELCKYSSEREQLATEAERASVKYKM
                     VEFMQDKVGMEFNGNISGITEWGIYVELEDTKIEGLVALRNIKEDYLEYDEDSMSLTG
                     KRTGRKFLLGDKVRIKVDRANLEQKELDYSLIWDIPH"
     gene            16785..18302
                     /gene="gpmI"
                     /locus_tag="P5293_00200"
     CDS             16785..18302
                     /gene="gpmI"
                     /locus_tag="P5293_00200"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRR48386.1"
                     /translation="MDKRVLLMILDGWGEGKHDKSNVIYTQGAPNIDAIRAKYPFSKL
                     SACGEDVGLPEGQMGNSEVGHLNIGAGRIVYQDLVKINKACREKRLMENPEIKAVYEY
                     VKNSGKALHFFGLMSDGGVHSSMEHLFGFLDVAKEMGLTKVYIHAFMDGRDTDPRSGK
                     GFIDQLLRYIQGTNIKLASIIGRYYAMDRDKRWERIKLAYDLITEGKGEGFTYGVDAM
                     QKSYDEGITDEFIKPHIALGEDGKPVGVLDEGDAVIFFNFRNDRARELTTVLTQTDML
                     EVGMKTLPLYYCTLTPYDEKFKGLHVLYDKENVSDTLGEVVSKVGKKQLRIAETEKYA
                     HVTFFFSGGREEKFENEFRILINSPKVATYDLQPEMSAPQVKDALIVELEKREYDLVV
                     LNFANGDMVGHTGVYEAIRKAVKTVDECASEVVKVARENGYSVIITADHGNADYALNP
                     DGSPNTAHSLNPVPFIVVDDDVTSVKDGILADIAPTILKLMGIPAPEAMTGKVLI"
     gene            18318..21905
                     /gene="dnaE"
                     /locus_tag="P5293_00205"
     CDS             18318..21905
                     /gene="dnaE"
                     /locus_tag="P5293_00205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRR48387.1"
                     /translation="MSFVHLHVHTQYSILDGAANIYKLFLKASEDNQIALAITDHGNM
                     FGVKEFLNTAKEFPNVKPIVGSEFYLTREGRFNKRGKEDQGAYHLILLAKNLNGYKNL
                     IKLSSYAYIEGFYYKPKIDRELLERYSKDLICTSACLGGEVPQAIIGWNLREAEETIL
                     WYKNLFGEDYYLELQRHETDIPGANNTVFVTQQFVNEEIAKLAVKHGIKCIATNDVHF
                     VNRDDGDAHDRLICITTNSDLDDPKRLRYTKQEYLKTQEEMGAIFNDMPQVLDNTIEI
                     ANKIERYSIESSPILPIFPLPEQYSDSNAYLRDLTYQGALNRYGTITPEIRERIDFEL
                     DTIKKMGYPDYFLIVQDFICAARTNGVSVGPGRGSASGSVVAYCLTITDVDPLKYNLL
                     FERFLNADRISFPDIDIDFDDDGRSKVFKYVEEKYGKDHVAHVITFGTMGAKTAIRDV
                     ARIQKLPLSEADRLAKLVPMRFGEDENGDKIEVSLENCIANVPEIAEAFNSKDPLLRT
                     TMEYAKKLEGNVRNTGVHACAVIIGRDDLREYIPICTSKDKETGEDIWVSQYEGAHIE
                     KVGMLKMDFLGLKTLSIIKDALDNIKKTRHIVLDIDTIPLDDPATYELFSRGDTIATF
                     QFESDGMRKWLKELKPNRLEDLIAMNALYRPGPMNYIPDFVDRKQGRKKIEYDLPGME
                     EILAETYGITVYQEQVMLLSQLIGGLTKGDADQLRKAMGKKDKRLMDDLHTKFMAGGE
                     ERGHSTEVLDKIWRDWTAFAEYAFNKSHSTCYALIGYRTAYLKANYPAEFMAATLSRN
                     LNDIEEITKLMDECKRMGIRVLGPDVNESSNNFTVNKRGDIRFGMAGIKGVGSMAVES
                     IVKAREETGEPFSSLFDFFERINLGIVNRKTLESLIFAGALDCFEEVKREQYMLPTSK
                     EDTFLDALIKYGVKYQNDIVNSGNSLFGDSETIKLVYPEIPPLTTESNKYDLLNREKE
                     LVGMYLSAHPLDQFAFEVKHFTTNTLSEIGELIEKASKDSGIQGKELIVAGMTTNVRI
                     SYTKNGSKPFANFTIEDYGGTYSFTLFSRDYENYMQYMQPNLPLLIKGAIMPKFSYDI
                     GKSEAPKNFEFKIKGIRHLANTREDFIRQITFNIPINLLTADLRKKFVNKIKENPGNT
                     MVIVNILDYKNKISVEYFSKKYKVSLNQELLDYLDSLSIGYTFTPTLSF"
     gene            complement(21867..22895)
                     /locus_tag="P5293_00210"
     CDS             complement(21867..22895)
                     /locus_tag="P5293_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRR48388.1"
                     /translation="MTAEKNKGRTSIEKVGKINLIDHLLSKANNSISDSISLTDENCC
                     WISSHKLLLEGIDFDLVYTPLKYLGYKSVLKSFGTLMAHNFHPYGVSLNIGLSKRFCV
                     EDIEELWAGALAAIEEYNISLVSLDLSSSLTGLTISISSQGKQKRELFVQTPKVTSGD
                     LLCITGNLGAAYMGLQLLEREKTVFNNNPMLQPKLDGYKFILKSYLNPQLDISIMEIL
                     SNNGIIPSDGYFITNGLADSVKFLCKKHNMGAKIYLNRIPIASETFEMAAEFNIDAIT
                     AALNGGDDFQILYSVPLSKYEELKRELPQIDIIGHLTSSINSPVLVTPDGSEIELKAQ
                     GWSESISD"
     gene            22953..24386
                     /gene="sufB"
                     /locus_tag="P5293_00215"
     CDS             22953..24386
                     /gene="sufB"
                     /locus_tag="P5293_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRR48389.1"
                     /translation="MNADDIIQKVTETDYEHGFETVLDQEYFPKGLNEQIITDISRRK
                     GEPEWLLEFRLKAYAKWLTMKMPTWANLVIPKIDFQDIIYFAAPKKSGSGATLDPELQ
                     TTFDKLGIPLDERNVLAGVAVDAVFDSVSIKTTFRESLAEKGIIFCSFSEAVRDYPDL
                     VRKYLASVVPFSDNYFAALNSAVFSDGSFCYIPKGVRCPMELSSYFRINARGTGQFER
                     TLIVADEGSYVSYLEGCTAPVRDENQLHAAVVEIVVLKDAEVKYSTVQNWYPGDAQGN
                     GGIFNFVTKRGICKGDNSKLFWAQVETGSAITWKYPSTILRGDNSISEFYSVAVTNHY
                     QQADTGTKMIHIGKNTRSRIVSKGISAGFSQNSYRGLVKVLPNAEGARNFTQCDSLLL
                     GDKCGAHTFPYIENENETAILEHEATTSKINDDQLFYCQQRGIGMEDAVGLIVNGYAR
                     EVLNQLPMEFAVEAQKLLQVTLEGSVG"
     gene            24395..25048
                     /gene="pnuC"
                     /locus_tag="P5293_00220"
     CDS             24395..25048
                     /gene="pnuC"
                     /locus_tag="P5293_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRR48390.1"
                     /translation="MEIFSVDNIIFSLGGQGVSLLEFISVLTGLTCVFLATRSKVANF
                     WVGYLYNILLFILFYQKGLYSSMIVQPISFAINFFGHYRWTHPRKGEENSKHQLKISL
                     MSNGKRLFFLLLILLLGAVWGTALTYFNDIWPSIFREARIPYLDSVITFTILTAQYLS
                     AQKRLECWVAWFIVNTTNIILYILAGLVFMPLVSAAYLVIAFFGFSMWLKEWKSYSN"
     gene            25060..25803
                     /gene="sufC"
                     /locus_tag="P5293_00225"
     CDS             25060..25803
                     /gene="sufC"
                     /locus_tag="P5293_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRR48391.1"
                     /translation="MLEIKGLRASIEGKEILKGIDFSVNEGEVHAIMGPNGSGKSTLS
                     AVLAGRENFVVTAGEVIYLGENLLDLAPEERAHRGIFLGFQYPVEIPGVSNVNFMKTA
                     INEKRKFANLPPLSAAEFLKMMREKMAVVEMDSSFSGRGVNVGFSGGEKKRNEIFQMA
                     MLEPKLAILDETDSGLDVDALRIVATGVNKLKRPDNAFIIITHYQRLLNYIVPDIVHV
                     LYDGKIIKTAGKELALEVENRGYDWLING"
     gene            25807..26847
                     /gene="sufD"
                     /locus_tag="P5293_00230"
     CDS             25807..26847
                     /gene="sufD"
                     /locus_tag="P5293_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRR48392.1"
                     /translation="MALQYKYNPAIPDVFRCRVPLPGTHQAYLSNGLLHEEVSYEDKL
                     HLSASSPMGFDLKISPGAQIPETLQIISVRETGQTVDLQFSNLLDFGAGSDSNVLLCT
                     HTLSLDEFVTLSELNINIQQGSRVNIVIMQNEHNGSSHKMNFKIKQAQDSYLRLNIVS
                     LHGARLENNIDVNLEGEGGICELNGVYLVSGKQEILTYVNMRHVVPNCHSSQLFKGIL
                     DNESRAVFSGLINVAKGAQKTEAYQANHNLLLSRKAKVYAQPHLEINADDVKCSHGAT
                     SGRLDELALFYMRSRGIGLEEAKLLQQLAFIYDVLEKVNNVPLRNRLQELVESRLRGE
                     FEQCANCSINCC"
     gene            26971..29493
                     /locus_tag="P5293_00235"
     CDS             26971..29493
                     /locus_tag="P5293_00235"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRR48393.1"
                     /translation="MNTYTYEEAMAASLAYFKGDELAASVWINKYAMKDSFGNLYEKT
                     PEDMQWRLANEFARIEEKYPNPMTAQEIFELLKEFKYIIPQGGPMTGIGNGYQIASLS
                     NCFVIGHTKPADSYGGIMRIDEEQVQLMKRRGGVGHDLSHIRPAGSPVLNSALTSTGI
                     VPFMERYSNSTREVAQDGRRGALMLTISIKHPDSEDFIDAKLDQGKVTGANISVKIDD
                     EFMRAALQGKPYKQQFPIDSDNPKVVKEIDASALWKKIIHNAWQSAEPGVLFWDTIIR
                     ESLADCYADLGYKTVSTNPCGEIPLCPYDSCRLIAINLYSYVDKPFTPEAKFNFELFK
                     DHVQKAMRLNDDLIDLEMEKIDQIIAKISADPEEEEVKEAELNLWKKIQKKTLGGRRT
                     GLGITAEGDMLAALGLIYGAEKAIDFAVEVQKTLALEAYRSSNIMAQERGAFPIFDAR
                     REENNPFIQRIKREDEELYDLIVRYGRRNISMLTIAPTGTTSLMTQTTSGIEPVFMPV
                     YKRRRKVNPNDKNAVVSFKDEVGDCWEEYYVFHHKFLTWCQVNGYDKDKVMSMKGPQI
                     DALVAKSPYYKATSNDIDWLAKVKMQGRMQKWVDHSISVTVNLPNSVSEELVADVYKT
                     AWEYGCKGVTVYREGSRSGVLLSAKDKDTKPSVRKVERPKSLEAEVIRFKNGPEQWIA
                     LVGLMNGAPYEIFTGLLDEDTRNIPKSVTMGYIVKEKDEVTGKSRYDFQYIDRYGYRN
                     TVGGISHMFNKEYWNYAKLISGVLRNGMPIVDVVNLVHGLQLDSEAINTWKNGVERTL
                     KRYIPDGTKDDTGAKCEKCGSSELVYQEGCLCCMNCGYTKCG"
     gene            29493..30272
                     /gene="ispE"
                     /locus_tag="P5293_00240"
     CDS             29493..30272
                     /gene="ispE"
                     /locus_tag="P5293_00240"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRR48394.1"
                     /translation="MIVYPCAKINIGLKITGKRSDGYHDLETFFFPVELTDILEVVEA
                     EETILYVYGKKPDCSMEDNLCYKAYQLLKRDFNLPPVEIHLFKRIPYGAGLGGGSSDG
                     SNMLIALNKLFNLGLTDHQLCKYASKLGSDCPFFIYASKLNSKAGEGIYAEGRGDILT
                     PMIIPDLANHKIRLDFSDIYVSTAEAYAGLNPTRQTESLKKLLGYPISEWSKRIINDF
                     EEPVFNKYPVLAEKKQKFYEEGAVYASMSGSGSAIYGIFAT"
     gene            complement(30383..31609)
                     /locus_tag="P5293_00245"
     CDS             complement(30383..31609)
                     /locus_tag="P5293_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRR48395.1"
                     /translation="MNFSFFIARHYLFAKKSHNVINIISLISAVGIMLGTMALIIILS
                     VYNGFDGLIRSLYSVHECDLLIVPAKGKSFVPKSVTFDQIKGSSMVANFCEVVEENVF
                     VKYDQEEAIATIKGVDSSFEKVTALKNYVVEGEFSMYHGEIPQAIVGRGIAQKIGLKV
                     FFLNGIDFYFPSRFRPVSFINPISSLNSERVYPAGIFSIEKEFDDKYIYIPIDVARNL
                     IEYDNGEVTSVELYLKAGINPEKAKAQFSLLLGNDFIIKNRYEQNETLYKMMGSEKLS
                     IYLILLFVIIIISCNLFGSLAMLIMDKREDVGVLKSLGANEKCIKNIFFYEGCLISFT
                     GIIAGIVAGLIICLLQQQFGFIKMPGNFIIEAYPVVIKWSDILATFLGVSLIGLFASK
                     LPQRLFRKIIESKEIS"
     gene            complement(31611..31955)
                     /gene="rbfA"
                     /locus_tag="P5293_00250"
     CDS             complement(31611..31955)
                     /gene="rbfA"
                     /locus_tag="P5293_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRR48396.1"
                     /translation="MQRESTRQLKVAKQIQKDLAEIIREKGMAFFDGAMVTVTSVRVS
                     PDLSIAKVYLSVFPSTKAKDVFKIIDSKSKSIRGELGNRVSKQLRIVPELEYFIDDSL
                     DYVEKIDELLKK"
     gene            complement(31959..>32615)
                     /gene="tyrS"
                     /locus_tag="P5293_00255"
     CDS             complement(31959..>32615)
                     /gene="tyrS"
                     /locus_tag="P5293_00255"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRR48397.1"
                     /translation="RKEGGDAFALTCPLITKADGGKFGKTEKGNIWLDARYTSPYTFY
                     QFWLNVSDEDAQKFIKIFTMLTKEEIEETIENHLKAPHLRELQKLLAKEVTVMVHGYE
                     EYEKALDASQILFGNSTYEKLKNIDEQTFLSVFEGVPQFEISRTRLPLNIVELLSIHC
                     NIFSSKGECRKMIQAGGLSINKERCETTDLQITENELLNNKYILVQKGKKNYFIIKLL
                     "
     assembly_gap    32616..32715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32716..33113)
                     /gene="tyrS"
                     /locus_tag="P5293_00260"
     CDS             complement(<32716..33113)
                     /gene="tyrS"
                     /locus_tag="P5293_00260"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRR48398.1"
                     /translation="MNNFVQELTWRGMIHDIMPGTEEMLNKESASGYVGIDPTADSLH
                     IGHLVSIMMLRHFQACGHKPIVLVGGATGMIGDPSMKSAERKLLDEETLRYNQEAIKK
                     QLSRFLNFKSDSPNAAIMVNNYDWMKDYSF"
     gene            33182..>34432
                     /locus_tag="P5293_00265"
     CDS             33182..>34432
                     /locus_tag="P5293_00265"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HRR48399.1"
                     /translation="MKKTIRKIGILFIAAGFLLCASPSTATAQNQSQDTLKELISSIP
                     GISGVERLKTDQFKDKYLLFVEQPIDHKNPDLGSFKQRVFVMNAGLDKPTVVVTEGYG
                     GAYAKMPRYREELSSIFDANIIFVEHRYFLESTPEPLDWKYLTAENSANDLHNVVTLF
                     KNIYKGKWIATGISKGGQTSLIYRAFFPEDVDITVSYVAPLCRSREDGRHEPFIEKKV
                     GTSEQRATILNFQKEILKKRNRLQPKFDSLCLSNKYTFNLPLNNIYDYSVLEFSFSFW
                     QWGNSIYEIPDSTASDDQIFKYWMKISSPDYFVNNSSSTPFFVQAARELGYYGYDTKP
                     FKELLSIKNAEGYLTKLFLPEGSEFKFDKTLYKKLKRFVATTDKKMLFIYGEYDPWSA
                     VKVNEPKHKNVVVMVQPKGSHRSRI"
BASE COUNT        10686 a         5939 c         6719 g        10988 t
ORIGIN      
        1 gttgtcgggg tactccgact cgaacggagg accccctgct cccaaagcag gtgcgctagc
       61 caactgcgcc acaccccgat taattcaaac aacaataaga acagactttt cttttagggt
      121 tgcaaagata aatagtataa ataatattac aaaattttta ataacgcctt ccttatgaaa
      181 aagcgacatt gtggtttaaa ttagaagcgt attatttttt tgagttttct ttaaaagtag
      241 ttaactttag tttataaacg ctctaaaaat tcagtgttac tagataaata acatcgtatt
      301 tatgaaaaat cattttttta caacatcaca tgaatcgctt atcagaggta ttattgcctt
      361 gattataggc ggactggctg tttttatgcc ggatattaca ttgcggagtt ttgttatctg
      421 catcgggata ttgattttgg ttagtggaat agtctctttt gttttttctc ttcgcagtca
      481 ggtgagagtt actaaaaaca tctttacggc aaatgcctta ttcaatctta ttttcggatt
      541 gctttttgta ctgttgccaa caatgatggt taatattttt gtcattgcca ttggcattgg
      601 ttttctgtta ataggtattt ttcaattaat cagcgcgtta agtattcgac acgattatgg
      661 gtggtcatgg atttatttct ctatttcaat gctaatgatc attggaggaa ttgttctgtt
      721 taccaatcct tttactagcg cacagactat tctgatcttt attggcatat tttcgctgat
      781 ttacggtgtg acagaactgt acatggcatg gaagctaaat aaaaagctta accattaatt
      841 aaataaagtc acaatttgtg gctggaattg caaatctaat tggcctattt aagctagcaa
      901 ctccaatatt acagattagt aagttatagt ctcgtttgat ctaattacag aatttgctct
      961 ttctttaaat gcgcctgtct taccacctat aaggaattta taggtcaatg agaaaaggaa
     1021 gtattgacgg acagtgttat agcgcgtctc ctggatatag aaatcggtta cctctcgttg
     1081 aatgttttta ttttgattaa ggatgtcatt tgcagagaag cttattgtca gcttgttttt
     1141 gattaaaggc ctggaaattg ctgcattcca gagaagatat ggcttgtcgt actgagctgt
     1201 ataaccttta aagtactgat aagcgatgtc tgatttaaaa gtaatatcaa aaggcatcga
     1261 atagctgata tatccctcaa ttacatttct ccaagttttt gtttttttct ctggaaagat
     1321 tgaatttaca gatagctcat aattaaatgt agcttttgtc tgaaaatata ttttatccag
     1381 tttcaaagtg agattaagac tttctcctaa gacattcctt ttagttgtgt ttttctctcc
     1441 gttgacaaag gaaatattat tataaagccc tccgtttata atatttgcaa ttgttaaaac
     1501 tcctttgaag aaaggttgtt cccagtgcag gtttgaagtt atgaaatagt ctccgctgta
     1561 gttgattggc atagtatatt gaattccatc ttcatcaaac catgatttat tcaaaattct
     1621 gtttttaaaa taagatgctt ttgttgttaa aagaatacca taaccaaatc cacctgaaag
     1681 cgatgtgatt tttctaatag atagattaac attgtgctca tactcgcttt ttaactctga
     1741 atttcccaat ctgattaata aaggatttgt gttatccgga atgggcatca ttaatgttag
     1801 agaaggagtc cttacatttc ctgaatattt aaggaaaata gagagaaagt ctttagttgt
     1861 caatctatac tcagcatttg gagaaatatt ccagaaccat ttgtcaaatt cttgttgatg
     1921 actatctctc tttaaatatg atggcaaaat tgatgcccca agcataaaat agctaacttc
     1981 atcttttctt ttgggtttct gaagatagaa ctcaatattt tgtgttaact caatattgtt
     2041 tgagttagca gacaaaagcg aatcaagttc tgaataattg ccgttattat ctgaattata
     2101 agtctcttta ctctgcgaat atattgagga tgctatactg tagtttgagc cgaatattat
     2161 ttttttagta attggctcgg tataggatac tttggccgat acatcaaagg atttgtcatc
     2221 agtaaagtac tgttgatctg atctgttcaa tattttattt ttcccttcgc tctcccgttt
     2281 atctatccca acagtccctt ttatgctgaa tgtcctcata ctcttgcctg tttttgtaat
     2341 atactggata tctgtcgaaa aattttcaat agacctccta ccagagttta cactctctcc
     2401 ctcgttgata gtaacacccg agaaaccgcc gattgtcctg tattttacag aatctgagta
     2461 attaccatat accatcataa cttttggatt tattgatatc ttgctctttt taccgctcca
     2521 attaatttgt ccagaaagaa gatggtttat atttgaattg tcacttctgg agcttttatc
     2581 ataaacgaat atactgtctt ttaaaaagtt tgtcctgtga acttcattat cattaaaaat
     2641 ggatttatcg tttagcttgt atgaaatatc tgcttcaaaa ttattcttct tatttgaaag
     2701 gtttatattg ctcccaattg agtaatttct tgataaaccg gatgatactg ccacgcctgg
     2761 catcataggg gcttcgttca ggtttgatgc atttccaaca attgcaattt gattgtattt
     2821 gttgaagtta gcaataaagt catttgtctt ataacgctta tctgttcctc cgccagcctt
     2881 aatgcttcct acccatccgt taagcatatc ctctttgatg gtaacatcta cagtatttgt
     2941 cctggcaccg tcatcaattc ctgtgaacgt tgatctctca gactgctttt caaacagttt
     3001 tatgtttttt aataattttg caggcagatt acggctgaca aatacagggt cgttcaggaa
     3061 aaatattctt ccattaacag ttatctgttc aattacttta ccattggctg tgatttttcc
     3121 atctctatct acactccatc caggcagctt cttaaaaaaa tcttcaagaa catcggtgtc
     3181 tgttagtttg aatgctgctg cattatactg aatagtatct cccagatatt ttattggagc
     3241 aacatgctcc ctgatggtca ctgcattgag ttcaacaaca ttttcacgta atttgataac
     3301 accaaggtcg aatttattat tgccttcaat caactctttt acatcaattt taacagggac
     3361 aattttcata aaatagccca tatactgaag agaaatctca tagtcagatg cgggaattct
     3421 attgaatttg atgtttccgg agctgtcaga aattgcatag gtgaatatct ccttcttgtg
     3481 atttttgtca attttagaca atacagccgt taccaattcc aacggtgcat ccgtgagact
     3541 atctctgaca gataaagtaa tatcggctct caggcttccc cttggctgtg agaatgaaga
     3601 aaatgaaagc agaataatgg ctgtgagtag tccaaatttc tttatcataa ctacatttcc
     3661 ctcttttttg ctgctttttg cgtacgtgtt tattgtactc taaaagtata ccaatttaaa
     3721 ataatctcca agtccgtttt atggtttttg gggtacccca aaaaccataa aacaggtgct
     3781 cttattttca ctaccgtttt catttttcac caatcatttg tctggtttct ttcagtctgt
     3841 atgattttcc gttcttatct atccgataag ccttatgggt taatctgtct accagagccg
     3901 ctgtcaatac gggatcgatt tgtcaaaaaa accaggagcc gggtcgtcgg agagcttaaa
     3961 gaaaaacaca aaggagtgag tgtcgagaat ctctgcgacc gattcggcta ttcgagacaa
     4021 gcatactaca aatccaaagt agtccaagaa agatacatag tggacgaagc agtaatcatc
     4081 tcagcagttg aagagatcag aagcgaaaaa gattttccca ggcttggagg tcagaagttg
     4141 ttatcgatgt tgataggttt aggttttgat atagggcgcg acagcctatt cgatatactg
     4201 cgctcaaaca aattattata gactaataac ctgtcaactt ttttcaggac gatacagaca
     4261 tgtatatctt atccggaaat agagtaaaca cttaagcaaa gaatgaaaca actaatgaag
     4321 cgaaggacaa aggtaacaat gaagaaaacg atgaaataaa gaagagaaga ataaagtgaa
     4381 caagtctgat agttttatta ctgtgttttt catttatcag cagggcacag gatgttgtta
     4441 aatgcgaaat cagatgtgag aaaatatccg gaaaacttat gaaattcact ttacccccaa
     4501 agtgcaggaa tcatggaaca tttattcttt ggaagtctcc agggggatga atgtatccac
     4561 cagcttctcc tttgaggata atccactggt catattgaaa ggagcagtga aagagtagtc
     4621 aagccaaata atacttactt ctctataatt ggtcaataat tctgtcacct gatttttaac
     4681 atattctcta tagacatcca tgtttttccc tttgttaaca gctgcatagg cggaatcaga
     4741 tgtttgactt cgaggatgat ttctatctat ggtatagtcc ggatgatgcc agtccagcaa
     4801 agagtaataa aatcctattt ttataccttc ggcctggaaa gcataaccca ttcttttaac
     4861 aaatcctttc catatggggt gtttgtagat ttgtagtctg taaaccgggt atcgaaaaga
     4921 caaaaccctt catgtttttg aatatcactc tctttgggcg tttgggcaat aatcgtattc
     4981 gttgctgata gtattaaatt gattattagc attattactg acaaacgtgt atgaaataat
     5041 ttgtttgcca ttataactcg ttttagttta acttatttgt catttgaatg aaaaagaaca
     5101 ttacttcttc ataacatctt ccggaagcaa cacatccgtc aacgttcctt cagggatatt
     5161 tttcaggaac agttttggaa taattgtatg gtttttggca aaaggtaatt tttgggcttt
     5221 ttcctttagc tctgccaaca gacgaccgct attctcttgg gttgtccatt tacactcacc
     5281 cacaagcaaa tattttttgt ctgatgactc tgcaacaaca tccaactcaa tccgttcctc
     5341 tcttgaaaca ttgccccacc aacgtcgggc aattccatat gttatcccgt ctagttcatt
     5401 ccctgaaacg gtttcacggc aaagcttttc ccaatactca ctcacaaagt cagaaaaatg
     5461 agcgtcgaga tctgccagta tcggagatat ccggcctatc tcaataaaag atctgttagg
     5521 aatgacaaac cggaaataaa agttcataaa cgggtcgctt attttataaa ggcttttctt
     5581 tgagtttttt tcattctctc caaaagggat ctctttggtc aggtacccta atgaaatcat
     5641 tttggcaagc ggtcttgaaa gatttgttgc aatttcaccg cttcttcccg ctatctcgga
     5701 catgcggtgc gctcctgctc ccacatacga cataatagtc gcagttttaa caatatcatt
     5761 aatatcgtct ttgaagagtt ttaaaggttc ttcatacaga gttccgttca ctgataataa
     5821 attgttttca atagcttcac gcagattagc atagccttcc ctcagctccc agtatcgtgg
     5881 cactccgccc caaacagcat actcctccac tgcttcaaca gccggaacgc caagagcgtc
     5941 ttgaatatat ggcactttta ttggcgtaat tttaagaatt gcatcggctc tcccataaag
     6001 tggagatgtt gcatccaaaa ccaaaccata cattcgctgt tgagaagaac cacaaataat
     6061 tagattgaat ttcaagcgtt tggaatctat tttgttctga agtatagatg gcagttcctg
     6121 gctgctttcg acaagatttg ggaattcatc tatacacaaa gtgaattttt gggaagtacg
     6181 gtcgttaaga gtatcaaaaa aaacattcca atccggatat atagccttat caaatccggg
     6241 gattatagat gctaccatct ttgccaggag ctttctttga agagtggcat cagtctggtc
     6301 tgctaaatag tatacatcat ttgcgccaag cgttctggta atcaatgtag attttccaag
     6361 tcgtctgcgc ccataaatga ctgtaaatgt ggatttgttt gcagtcaata cctgcttgag
     6421 ccttttactc tcttctattc tatcaacaaa tttcatatct caacatgtat tatgctctac
     6481 aaacataatg tttattttgc ataattccaa gaaacaggtt aatctccaac ttcctgacag
     6541 gaaaaactaa ccggaaccga gctcttcatc tcaaattagc aaaaagtttc cactatgaat
     6601 aaataataaa tgtatttgtc ataaaattgc cttttatatg atacagataa actttgacga
     6661 tggattgaca atagaagttg tgccggggtt taagttcctg aattgaggct gaaagcaaaa
     6721 ccagatatca tcgtattagc tctctgattt tacgataagc ccttaacttc agggttttga
     6781 ttgtgtttat agataacccc agcgcatctg caatcggatg agcataacag tatctcttcc
     6841 gattgtaagg atgagaaaaa ttacgtaact tcgtacaagt catcggacag tttttgttcc
     6901 ctaatgtaag ataagtgtca gagaaagtct tgattattac acaacgcaga aattcacgaa
     6961 aagtgaacaa atttgcagaa aggcttacaa aagaaattct acaataaaag agaccaaatc
     7021 tggatattat tacctgatta ggtctctttt ttacatcttg ctgtatttca gtaaccgttg
     7081 cggtgagggg gggattcgaa cccccggaac ccttgcgagt tcggcagttt agcaaactgc
     7141 tggtttaagc cactcaccca cctcaccgtt aattgggtat attgcttaaa ttaaatacct
     7201 cgcagtgcga ggggctgcaa agatactaat ttttactttt tttcaatagc ttcgacattt
     7261 atttcttcac ctgccactcc tttcaatatt gacaagagga tatctgcttc actcttgctg
     7321 ttaaaaggcc caatgaagta aacgcttttt ccagcaacag atttcatggc aatatctttt
     7381 tccgtattat ttctgataag ttcaagaaca tattgcggta tgccatcggg gaatgctgta
     7441 attttgactt gatatgtggc tgatgaattt atcttgtctt caagaagtct tgcatttttt
     7501 atactgatag tttttccctt ataataagca agaggtaaaa catctctgaa tcccattgat
     7561 ttaagctttt gtaaggcatc gcttaattct ttataggaat agaatctgcc tgcagtgtag
     7621 ttaaacttac cattttgtgt tttagtttca aatatgggtg atattccctt gaaagatgct
     7681 gaatcagctt ttgctgaaag cataaaaagc tggatagtat aaaaaagttc ttctggtata
     7741 tcctgttcat tttcgattag aacaaaatta ccacttgttc tgaatgagag atctactggt
     7801 ttcactggct caacaatttc catatatgga agttttccat aatgtgaaag ttttaactta
     7861 aacggttcag cctgattgtt gattattacc ggtttatccc acattccttc ctccctcggt
     7921 atgataacac ctttatttaa gaaatctatt tccttttgtt gtattgcctt atttgcaatc
     7981 atgagttttg actgtttttc cagaagaatc atctcgtctt cgccaatttt ctttatcaga
     8041 taatttttgt cgtcttcgtt gttcaaggtg gcaagcaaag ctctgttggc attaatagcc
     8101 cgaatatttt catcaatctc tttttgcaat tgtcgggcct ctttaatcat tctggtatat
     8161 tcgtttgttt cagtatcctt tttcaatgtg tctgcaggag ttccataatt attagctttt
     8221 aaatatgaac cagtatttaa agaagctatt tgtgctgctt cttcaataga ttgtacagga
     8281 cttctgacag gattggcttc aaatgaaaga atataaatag taatggaatc agtagatgca
     8341 caatttctgt tagaggcaaa tatggcataa tcccctccat cactctctac ataaagataa
     8401 tcatctgctg gagacgaata aggaaatccg aggttttgag gagtatccca tacattacgc
     8461 ttttcatccc aataacttac ataaatatca aatccaccca tcccggcgag tccattagaa
     8521 gaaaaataca gctttttgcc atcgtcgctt tggtatggta gaatttcatc ttcttgagaa
     8581 tttaaagtat ttacaggttc aggacttgac catttctcat tatctataca ctgagaagag
     8641 aaaatatccc atttcccttc ttgattttga gcggaaaaat atataatgtc cttattggat
     8701 gatagtctca tgacattatt tatgggataa cttctcatat ttttattgag tgtgtaagga
     8761 acaagaatcc aagaactgtc aggtatatca ggataataca agaaaaaatc cttaacaggt
     8821 acattagcag ctcctaatac tcttggacgt gcagcgtaat ctaacatctt aagtccattc
     8881 tcgcaatttg caatcctgga ttctactttt ctgttaaatt cttcatcttt acttatttca
     8941 aggagttgtt gatatattgc tattgcttcc tcaaaacgat agttttttct atgttcttca
     9001 gcagaaataa actttgattt aaggtcaggt gtttgcccga aagatagacc accagctagc
     9061 aggaaaagta atagcacgca gattgaccta ttcataatat caacaaaata ttgtagctta
     9121 caaaaatacg aatttcttta tatattggga aaaaatagta tttttgtgcc ttaaattcct
     9181 tggattgtat ataatttaaa aacagatatt catgcaaagt aaaggtgcca taaaattatt
     9241 ggcaatagtt atcgccctgg cttgtattta tcagctgtca tttacattgg cagtaaaaca
     9301 tcaagaaaag aaagcagttc aatatgccga gagggcagtg aagattgagc agacacttcc
     9361 ttctttccaa aatgttccag aattagatag agcctattat cttgactcct tgcgtaacct
     9421 caaggagaaa ttttatattg attctatctc tgctgagaag gtttatctgg gttttaccta
     9481 taaggaaata aaagaaagag aggtgagcct tggtcttgac ttgaaaggtg gaatgaatgt
     9541 tatgttggaa gttagtgtcg cagatcttgt taattcactt gccaataata acacttctcc
     9601 tcagttcata gaagccatga gggtagcccg ggaaaaccag gccaattcca agtcagattt
     9661 cattactctt tttgcagagg catgggacaa ggttgctcct ggacaaaggt tgtctcagat
     9721 attcggaaca tatgaaatga gagacaagat aaaaccagaa tccacaaatg atgaagttat
     9781 tgcagttata aggaaagagg ctgaaagcgc aatttctaac tcttttaatg ttttaagaaa
     9841 ccgtatcgac cgttttggtg ttacacagcc aaatattcag aaaattggaa atacaggacg
     9901 tattcttgtt gagctacctg gtgttaagga gccggagcgc gtcagaaaac ttcttcaggg
     9961 aactgcttct ctcgaattct gggagacata tgaaaatcct gaggtttaca aatatttggc
    10021 agaggctaat aatgtaataa aagattactt agcttcaggc attacaaaag attctctgtc
    10081 ttctactgat actttggcag caagtgcaaa atctgtttct gattcttcaa agagcgcaga
    10141 ggccgattca cttctttctc agattagagc tacagattct ctttctgttg atgataagac
    10201 tcttgccaag gacaatcctc tgtttttcct gcttaatcct aatgtttatc agggacagct
    10261 tatggaaggg ccatgtgttg gaagagctca ctacaaggat actgctaaaa ttggtgcatg
    10321 gctcaggttg ccacaagttc aggcagtatt tccttcagat ttaaagctat tatggacagt
    10381 aaaacctatt gataaagcag gtacaattta tgaacttata gcaatcaagg caaatactcg
    10441 tgatggcaag gcacctcttg atggaagtgt tataactgac gctagcaaat catttggtag
    10501 tacaagaggt actcctgagg tcgacatggg catgaatgcc gagggcgcaa gaacctgggc
    10561 tacaatgaca gcaaataaca ttggaaaatg tattgcaatt gttcttgatg ggatggttta
    10621 ctcttatccg agagtaaatt ctgaaatatc tggaggaagg tctcagatta ccggtaattt
    10681 tacagtacag gaagcagatg acttggtcaa tgttctcaaa tccggaaaac ttcctgctcc
    10741 tgcgagaatt gtccaggagg cagttgtggg cccgacactc ggtacagagt caattaatgc
    10801 cgggatgttg tcatttctta tagcatttct gcttgtgctg atttacatga tctttttcta
    10861 taatggtgct ggagtcgcgg cttgtgtatc tcttatagcc aacgttttgt ttttgctggg
    10921 tacactggtc tcgtttggcg cagtgcttac acttcctggt attgctggta ttgtgctgac
    10981 tatgggtatg gctgtggacg caaatgttat tatttatgaa cgtattaagg aggagttaag
    11041 agcaggtaag ggtttaagac ttgcagtgtc ggatgggttc agcaacgcat attcagcaat
    11101 tattgatggc cagttgacaa caatcatcac aggtatagtg ctgatgattt ttggaacagg
    11161 tccagtacaa ggttttgcta ccacacttgt aataggtatt attacatcat tagccacttc
    11221 tatctttatt ccaagattga tatttgaggg cagacttgct aagaataagt ctatttcatt
    11281 ctctaacaag ctcactcaaa actttatgag taataccaaa attaacttca tgaaagttcg
    11341 taaaattttc tatgccattt caatttcttt gatagtcata tctgttggtt caatggtatt
    11401 caaaggattc tcatatggtg ttgattttac aggaggtcgt acatatgtgg taagatttga
    11461 ccagaatgtt actacagagg aagttagaaa agcggttatg gatgtgttca atgaaggagt
    11521 tgaagttaaa cagtttggtg gagaaagtca ggtgaagatc acaacaaaat acaagataga
    11581 agataattcg tctgaaactg acgctttggt tgaccagatg ctttacaatg ctcttaaagg
    11641 tttctttgct acatctatta catttgaaga gtttactgct actaccgtaa atccaaatgg
    11701 tattatcagt tccgaaaaag ttggtcctac aattgcggat gatatgcaga gaaatgcaat
    11761 aatagcagtc tttatagcat tattagcaat atttgtatat atagccgtta gattccgtaa
    11821 atggacatgg ggaacaggag gtgtattggc acttttccac gacactataa ttgtaattgg
    11881 atttttctct ctgttctccg ggatactacc tttcagtctt gacattgacc aaacttttat
    11941 tgcagcgatc cttacgatta tcggatattc aattaatgat actgttatta tattcgacag
    12001 aatcagagaa tatagaagac tctatcctaa gagagatttg gcaattaata taaatgaagc
    12061 ccttaacagc accttagcga ggacaattaa tacagcaggt actacacttg tggttttgat
    12121 agcaattgct ctgtttggtg gagaggttat ccgcggtttt gctgtagcgc ttattcttgg
    12181 tgttgctatt ggtacatact cgtcagtatt tattgcaacg cctatcgttt atgatattac
    12241 cgggtggctg aacaaaaaga aggaagaaaa aaaacttgtg gttaatacag ggaagaataa
    12301 gaaataattt tatctgaaat aattttatta aaggagctga ccaaaatcta tatttttgag
    12361 tcagctcctt tttttattta actttgttga taaaatttaa taaaaagtaa taaaatggaa
    12421 ctaccttttg cagaatccta caaaattaaa atggtcgaaa gcattcgaaa gagtacacgt
    12481 tcgcaaagag agcagtggat taaagatgct cactttaatt tatttaattt aaagagcgat
    12541 cacgtattta tagatcttct taccgattca ggaacaggag ccatgagcga taaacaatgg
    12601 tctgctatta tggaaggtga cgaaagttat gctggtgcac gttcatatta taagttgaaa
    12661 gaagcaatag aggatataac cggttttcct tattttctcc ctacacatca ggggagagct
    12721 gctgaaaacg tccttttttc ttcaattatc aaagcaggag atattttgcc aggaaattct
    12781 cattttgata ccacaaaggg acatattgaa tttcgcaagg ctcatgcagt tgactgtaca
    12841 atagatgctg ctgccgatac aactcttgaa ctccctttta aaggaaatat ggatcttgat
    12901 aaacttgaaa atgttcttaa gaaatatcca aaagaacaga tcccgtgtat tgtaattact
    12961 gtaaccaata acactgcagg agggcagcct gtttcaatgg aaaacataaa aggcgtttca
    13021 gatttggcaa aacgctatgg ggtaaaagtt ttaattgact ctgcacgttt tgctgaaaat
    13081 gcatatttta tcaagactcg ggaaaaggga tatgaaaata aaagtataag ggaaattgtc
    13141 cgagaaatgt ttagctatgc tgattttatg accatgtcgt ccaaaaaaga tgcaatagtc
    13201 aatataggtg gttttattgg atttcgtcat gaaaaggatt ggataagttg tcagatgtac
    13261 aatattatgt ttgaaggcta tattacatat ggtggtatgt caggaagaga catgaatgca
    13321 ttggctgttg gtctttatga gggtacagag tttgattatc tggaaactcg tatttctcaa
    13381 gttgcatatt tgggccataa acttgatgaa tatggtattc catatcaaag gccttctgga
    13441 ggtcacgcta tttttctgga tgctaagaaa attctgactc atgttcctaa agaacaattt
    13501 attgctcaga ctcttggtgt tgaactatat cttgagtctg gagtaagagc agttgaaata
    13561 gggactttac ttgccgacag ggatcctgat accagagaaa acagatatcc cgagcttgaa
    13621 cttcttcgtc ttgcaatccc cagaagggtt tatacaaata atcacatgga ttatatagct
    13681 gcagctgtaa agaatgtttt tgacaggaga gaatcaatta acaaaggcta tattataaca
    13741 aaagagatgc ctataatgag gcattttaca attgaattga aacctgcata aatttacagc
    13801 tttttgtaag tcttttaatc agcccgttgc aaccatatca tatctgcttc atgcaggcat
    13861 acttgctctt ggccatgaaa aacctgcaag aaacaaaagt attagtgtgc aaaacaagaa
    13921 tgttattaat cctgtttctc tgttttttac aaatattgat atgctcattg aaaagaatat
    13981 tgtagcaata agaaatggta tcattacgtg gagcagtgtt catgcagaac ctatatgggg
    14041 cagattaaac catcttgtta caagcacaag gatatatgct gtgattacaa tgtaaagcat
    14101 gaagcagctt atacctttac caatcaaggt ataaaaaatt tcatttcaca ataaaaataa
    14161 cgtgaatttt tacaacatat tgattgaaga aaacaatctc tgataatatg ctgcctttat
    14221 attaagaggc attggataag cgcgcgatga tgatggtagg cgataaagtt ttatttggcg
    14281 attttccaat tgaaaaaaag aaaagtttcc tatttccggt tctttgcaat tagcggtttt
    14341 taaaattata tcaagcgctt ttttccctgt ggcagctatt gtattgcatt tcggaatctt
    14401 aagtaaaagc tcttttagat tgagcatatc aactatttca agataattgt cagaggcatt
    14461 gcctctaagt cttttaactg ctgtggctgt atcatagatg gcaattccgt tcttatagca
    14521 aaaatccttg attttaacgg catcaaattt tttatttgta ataaaatatt ctttctcttc
    14581 gtggaaaact attccaaaaa tcctccacat atcattttga aaatttggat agaagaattc
    14641 catcgaccat ttatttcgtg ggggaggaaa acttccgagc attagaattt ttgcttctgg
    14701 aggtaaaaat ggttctaagg gatgaaattc aatgtacatc aagcagtcag tggggtatat
    14761 cccatattaa tgaatagtcc aactctttct gttcaaggtt tgctctgtct acttttattc
    14821 taactttatc acctagcaag aattttctgc ctgttctttt gccagtaagg gacattgaat
    14881 cttcgtcata ctcaagataa tcctctttta tatttctcaa agctactaat ccttcaatct
    14941 tggtatcttc gagttcgacg tatattcccc attcagtaat cccggaaata ttcccgttaa
    15001 actccattcc taccttgtct tgcataaact ccaccatctt gtatttcaca ctcgctctct
    15061 ctgcttctgt cgcaagctgc tctctttcac tggaatattt gcataattct tcataatatt
    15121 ttttttcctg agactttgca ttgtccaaat acattgacaa gagacgatga accatcatat
    15181 cgggataacg ccttataggt gatgtaaaat gtgtgtaata atcaaatccc agtccataat
    15241 ggcctacatt atcggtagaa tatttagcac gtgccattga gcgaagtgcc attatttcaa
    15301 ttgcttcctc ttcaggtttg ccttttgcag tttcaagcag tttattgatt tcactggaga
    15361 tttcacgact gtttttggtt ggtttcattt catagccaaa atggtgaata aaagtcctga
    15421 aagcagcaac tttgtcaaca tcaggttctt cgtggattct ataaacgaaa gtcttggcat
    15481 tttttccttt tttacctatt aaatatgcaa cttcacgatt ggcaagaagc ataaattctt
    15541 cgataagcca attggattcc ttggttattt ttgaatatac accaataggt ttgccatttt
    15601 cgtcgatttc aactttcatt tcaggtcttt caaaactgat tgctcctttg ctaaaacgtt
    15661 cttttcggag aattgtggca agtttatgaa gtttaagtat ctcttctttc attggtcctt
    15721 cacttccttc aattatgttc tgcgcttcat cataattaaa tctgtagtct gaattaatga
    15781 tagagcgacc aaaccatctg tcaataattt tagcattatc attcatgaca aatacggtcg
    15841 agaagcaaag cttatcttca ttaggtctta gagaacatag tttgtttgat agtttttcag
    15901 gaagcatagg tactgtcctg tcaacaagat agacagaagt agctctctcc tgagcctctt
    15961 tctcaataag tgatccgggt ttaacataat aagtaacatc agctatatga acacctattt
    16021 cccaattgcc gtcttcgagt ttgcgaatag atagggcatc atcaaaatct tttgcatctg
    16081 cagggtctat tgtaaaggta ggaacgtttc taaaatcttt tcttgatgaa atttcttcta
    16141 aagatatatt ttccggaatt ttatcagctt ctttttcaac ttgtacttca aacttatatg
    16201 gtagtccgaa ctcggcgaga attgcgtgca tctctgtgtt gttttcaccc ggagtcccaa
    16261 gaacatctat aatatgccca attggttcgt ctgatttcct aggccactca tcaacaactg
    16321 ctgcaacctt taatccattt tgagtatctt ttacattttc aatggggatt ctgatgtcat
    16381 agggcatatt tctgctttcg gttatcaccc aggcttgtga gcctgtaatc tgaagaactc
    16441 caataaacgg cttctttgac ctttcaataa tctcgatgat ttcaccctcc atgttgcgag
    16501 ttttggtctt tttagccgtc agagctactt taacagtatc tccgtttaaa gctcctctca
    16561 ttttacgggc actgacaaaa atatcatcat caaaaccatc ccttataata aatccataac
    16621 cttcacgtgt catgcttacg cgaccaatta cctcttctct tgggctcctt ttcgtgtttt
    16681 tatttttttc tttaaacttt tttgttttac ctgcgtttct gtttttactc atttttttgt
    16741 ataattttgc taaatccaat atggcaacaa aaataaataa atctatggat aaaagggtac
    16801 ttttaatgat ccttgatgga tggggtgaag gcaaacacga taaatccaat gttatttata
    16861 ctcagggggc accaaacatt gatgctatta gagctaaata tcctttctcg aaactttctg
    16921 catgtggaga agatgtagga cttcctgaag gacaaatggg aaattccgag gttgggcatc
    16981 ttaatattgg tgcaggaaga atcgtttatc aggacttagt taaaattaat aaagcttgca
    17041 gggaaaaaag actaatggaa aatcccgaga ttaaagctgt ttacgaatat gttaaaaatt
    17101 caggaaaggc tcttcatttt tttggtttaa tgagtgatgg tggcgtacac agttccatgg
    17161 aacatttgtt tggatttctt gatgttgcca aagagatggg ccttactaaa gtatatattc
    17221 atgcttttat ggatggtaga gatacagatc caagaagtgg taaagggttt attgatcagt
    17281 tattaagata tattcaaggg actaatatta agctggcttc cataatagga cgttactatg
    17341 ctatggatcg agacaagagg tgggagagaa taaagcttgc ttatgatctt attactgaag
    17401 gcaaaggtga ggggttcaca tatggcgttg atgcaatgca gaagagttat gacgagggta
    17461 taactgatga atttataaaa cctcatatag ctcttggtga ggatggcaaa cctgttggtg
    17521 ttcttgatga aggtgatgca gtgatatttt ttaattttcg caatgaccgt gctagagagt
    17581 tgaccacagt gcttacccaa acagatatgc tcgaagtggg gatgaagact cttccactct
    17641 attattgtac acttacacca tatgatgaaa agtttaaagg acttcatgtt ctatatgaca
    17701 aggaaaatgt gagcgacaca ttaggagaag tcgtttcaaa agtcggaaag aaacagttaa
    17761 gaatagcgga aacagaaaaa tatgcacatg ttaccttctt tttttcagga ggcagagagg
    17821 aaaaatttga gaatgaattc agaatattaa ttaattctcc aaaagttgct acctatgatc
    17881 ttcaacctga aatgagcgct cctcaggtaa aagatgcgct tatagtggaa ttggaaaaga
    17941 gagaatatga tcttgtggta ttaaactttg caaatggaga catggtagga catacaggag
    18001 tctatgaagc tattcgcaag gcagtgaaga ctgtagatga atgtgcttct gaggtggtaa
    18061 aggttgcaag agagaatggg tattctgtaa ttataactgc agatcatggt aatgccgact
    18121 atgcactcaa tcctgacgga tctccaaata cagcacactc tttaaatcct gttcctttta
    18181 ttgtggttga cgatgacgta acttcagtaa aggatgggat tcttgcggac attgcaccaa
    18241 caattcttaa gttaatggga attcccgcac ctgaggctat gaccggaaaa gtattgattt
    18301 aaaaaacaac ctgttaaatg tcatttgttc accttcacgt tcatactcaa tattctattc
    18361 ttgatggtgc tgccaatatt tataaacttt ttctcaaagc atctgaggat aatcaaatag
    18421 cacttgccat aaccgatcat ggaaacatgt ttggtgtaaa ggaatttctc aacacggcca
    18481 aagaattccc taatgtaaag ccaattgtag gctctgaatt ttacttgacc agagaaggtc
    18541 gtttcaataa aagaggcaag gaggatcaag gtgcatatca tcttattttg cttgcaaaaa
    18601 atctcaacgg atacaagaat ctgataaaac tgtcatcgta tgcttatatt gaaggttttt
    18661 attataagcc taaaattgac agggagcttc ttgaaagata ttcaaaggat ttaatatgta
    18721 catcggcctg tttaggtgga gaggtgccac aggcaattat tggatggaat cttagagaag
    18781 cagaagagac tattttatgg tacaaaaacc tgtttggaga agactattat ctagaattac
    18841 aacgtcatga aaccgatatt cctggcgcaa acaatactgt atttgtcaca cagcagtttg
    18901 taaatgaaga aattgctaaa cttgctgtta aacatggtat caagtgtatt gctacaaatg
    18961 atgtccattt tgtgaatcgt gatgatggag atgcacatga caggcttatt tgcatcacaa
    19021 ctaattcaga tcttgatgat cccaagagac tgcgatacac aaaacaagag tatcttaaaa
    19081 ctcaagaaga aatgggggca atctttaatg atatgcctca ggtattggat aacacaatcg
    19141 agatagcaaa caaaattgaa agatattcaa ttgaatcgag tccgatactt ccaatatttc
    19201 ctttgccaga gcaatattcc gattcaaatg cgtatttaag ggatcttacg tatcagggtg
    19261 ccctaaatag atatgggact attacacctg agattagaga aaggattgat tttgaattag
    19321 atactattaa aaaaatgggt tatcccgact attttctcat tgttcaggac tttatttgtg
    19381 ctgccaggac caacggagta tcggtcggac caggtagagg ctcagcttca ggttctgtag
    19441 tggcttattg ccttacaata accgatgtgg acccattaaa atacaattta ctttttgaaa
    19501 gatttcttaa tgctgacaga atatcttttc ccgatataga tattgatttt gatgatgacg
    19561 gaagatccaa ggtgtttaaa tacgttgaag aaaaatatgg caaagaccat gttgcacatg
    19621 ttatcacttt tgggactatg ggtgctaaaa ctgctatcag agatgttgca aggatacaga
    19681 aacttccgct cagcgaggca gacagacttg caaaactggt tccgatgaga tttggcgagg
    19741 acgaaaatgg tgataagata gaagtttctc tggaaaactg catagcaaat gtacctgaga
    19801 tagcagaagc atttaattct aaggatcctc ttctaaggac tacgatggaa tatgcgaaga
    19861 aacttgaggg taacgttaga aatacagggg tacatgcctg cgctgtaatt attggacggg
    19921 atgatcttag agaatatatt ccaatttgta ccagcaaaga caaggagacc ggtgaggata
    19981 tttgggtatc tcaatatgaa ggggcgcata tagaaaaagt agggatgctc aaaatggatt
    20041 ttttagggct aaagacttta tcaattatta aagacgcgtt agataatatt aagaaaacaa
    20101 gacatatagt acttgatatt gatacaattc ctttagacga tcctgcaacc tatgaacttt
    20161 ttagtagagg tgatacaata gccactttcc agtttgaaag tgatggtatg agaaaatggt
    20221 taaaggagtt aaagcccaac aggttggaag atcttattgc tatgaatgct ttatatcgtc
    20281 cgggtccaat gaattatatt cctgattttg ttgatcgtaa acaaggaaga aaaaaaattg
    20341 agtatgacct gcccggcatg gaagagattc ttgcagaaac ctatggtata actgtttatc
    20401 aggagcaggt aatgcttctg tctcaattaa taggaggctt gacaaaaggt gatgctgatc
    20461 agctgcgtaa agctatgggc aagaaagata aaaggttaat ggatgacttg catactaaat
    20521 tcatggctgg aggagaagaa agagggcatt ctactgaagt gcttgataaa atatggagag
    20581 actggactgc ttttgcagaa tatgctttca ataagtctca ttctacctgt tacgcactga
    20641 ttggatatag aacggcttac ttaaaggcta attatcctgc tgagtttatg gcagcgacat
    20701 taagtcgcaa tcttaacgat attgaagaga taacaaaatt gatggatgaa tgtaagcgga
    20761 tgggcataag agttttaggc cctgatgtaa atgaaagttc caacaatttt actgttaata
    20821 aaagaggcga tataaggttt ggaatggcag gaattaaagg agtgggatca atggcagtag
    20881 agagtatcgt aaaagcaaga gaagaaacag gagaaccatt ttcctctttg tttgactttt
    20941 ttgaaagaat taatttaggg attgttaata gaaaaacatt ggaatcttta atctttgccg
    21001 gagcactcga ctgttttgag gaagtaaaaa gagagcagta catgcttccg acatccaagg
    21061 aagatacttt tctcgatgct ctcattaagt atggtgtcaa atatcagaac gatatagtta
    21121 atagtggtaa ttctttgttt ggagacagcg aaaccattaa gttagtttat cctgagattc
    21181 cgcccttaac aacagaaagt aataaatatg atttgctaaa tcgtgaaaag gaactggttg
    21241 gaatgtatct ttctgctcat ccattagatc agtttgcatt tgaggttaaa cattttacaa
    21301 caaatactct ttcagagatt ggagagttaa ttgagaaagc ttcaaaggat tcagggatac
    21361 agggtaagga attgatagta gccggaatga ctacaaatgt taggatttca tatactaaga
    21421 atgggtctaa gccatttgcc aattttacaa ttgaagacta tggagggact tacagcttta
    21481 cgcttttttc cagagattat gaaaattaca tgcaatacat gcagccaaat cttccgttac
    21541 ttatcaaggg tgctataatg ccaaaattct cttatgatat cggcaaaagt gaagcaccaa
    21601 agaactttga gtttaaaata aaggggatca gacatttggc aaatacaaga gaggacttta
    21661 tcaggcagat tacattcaat attcccatta acttactcac agctgatcta agaaagaaat
    21721 ttgtgaataa aatcaaggag aatcctggta atactatggt tatagtcaac atacttgact
    21781 ataaaaataa aataagtgtt gaatactttt ctaaaaaata taaagtatca ctcaatcagg
    21841 aattactcga ttatcttgat tcactatcaa tcggatatac tttcactcca accttgagct
    21901 tttaattcta tttcagatcc atccggagta accaaaactg gactatttat ggaggatgtc
    21961 aggtgtccta taatatctat ttgagggagc tcgcgcttaa gctcttcata cttggaaaga
    22021 ggtactgagt ataaaatttg aaaatcgtca cctccgttaa gagctgctgt aattgcatca
    22081 atattaaatt cagcagccat ttcaaaagtc tcagacgcta tcgggattct atttaggtat
    22141 atcttggccc ccatattgtg ctttttgcaa aggaatttta cagaatcagc aagtccgtta
    22201 gtaataaagt aaccatcaga aggaatgatt ccgttgtttg acaaaatttc cataattgaa
    22261 atatctagtt gaggattgag atatgattta agaataaact tataaccatc taattttggt
    22321 tgaagcatag gattgttgtt aaaaacagtt ttttctcttt caagaagttg caatcccata
    22381 taggcagctc caaggttgcc tgtgatgcat aaaagatctc ctgacgtgac tttaggcgtt
    22441 tgaacaaaga gctctctttt ttgttttcct tgggatgaaa tgctaatggt caagccggta
    22501 agtgacgatg aaagatccaa ggaaactaaa gagatgttgt attcttcaat tgcagctaat
    22561 gcaccagccc aaagttcttc tatatcttct acacagaacc gctttgaaag accaatattt
    22621 aatgaaacgc cataaggatg gaaattgtgt gccattaaag tcccaaagga ctttaaaaca
    22681 gatttataac caagatattt gagaggtgta taaacaagat caaaatcaat cccttctaaa
    22741 agaagtttgt gtgaagatat ccagcaacaa ttttcatctg tcaatgatat ggagtccgaa
    22801 attgaattat tggcctttga taaaagatgg tcaatcaagt ttatttttcc aaccttttct
    22861 atggaggttc tccctttatt tttttcagct gtcattgtca tgatattaat ttgttcacaa
    22921 aaatagttta aaagatataa ctttgcgcca caatgaatgc agatgatata atccagaaag
    22981 taacggagac tgactacgaa cacggctttg agactgttct tgatcaggaa tattttccta
    23041 aagggctgaa tgagcaaata attacagata tttcccgcag gaagggagaa ccggaatggc
    23101 tgcttgaatt cagacttaag gcttatgcta agtggttgac aatgaaaatg ccgacatggg
    23161 caaatctcgt tattcctaaa attgattttc aggatattat ttattttgca gctcctaaaa
    23221 aaagtggaag tggagcaaca ttagatcccg aattacagac cacatttgac aaacttggta
    23281 ttcctcttga tgaacgaaat gtacttgcag gtgtagccgt tgatgctgta tttgattctg
    23341 tatcaattaa gactactttt agagaatctc ttgcagaaaa agggattata ttttgttctt
    23401 tttccgaagc agtaagggat tatccggatt tggtgagaaa atatcttgct tcagttgtac
    23461 cattcagtga caattatttt gcagccttga attcagcagt ttttagtgat ggttcatttt
    23521 gttacattcc taagggagta aggtgtccga tggagctgtc cagctatttt agaattaatg
    23581 caagaggaac aggtcagttt gaaagaacac ttattgtagc tgatgagggt tcatatgtga
    23641 gctatctgga gggctgtaca gctcctgtca gagatgaaaa tcagctacac gccgctgtag
    23701 ttgagatagt agttctgaaa gatgcagagg taaaatattc aacagttcaa aactggtatc
    23761 caggagatgc tcaagggaat ggaggaatct tcaattttgt tacaaaacgt gggatctgca
    23821 agggagataa cagtaaactt ttctgggctc aagttgaaac cggttctgct attacctgga
    23881 aatacccgag tactatattg agaggagaca actctatttc tgaattttat tcggttgctg
    23941 taacaaatca ttatcagcag gcagataccg gtacgaaaat gatacatatc ggcaagaata
    24001 caaggagtag aatagtgagc aagggaattt cggcaggatt cagtcagaat agttacagag
    24061 gacttgttaa agtacttcca aatgcagaag gagccagaaa ttttacacag tgtgattcgc
    24121 tgcttcttgg cgacaaatgt ggagctcata ctttccctta tatagaaaat gaaaatgaga
    24181 cggcgattct tgaacatgaa gcaacaactt ccaagataaa tgatgatcag cttttttact
    24241 gtcagcaaag ggggattggt atggaagatg ctgtgggttt aatagtaaac ggttatgcta
    24301 gagaggttct aaatcaattg cccatggaat ttgcagtaga ggctcaaaag ctcttgcagg
    24361 tgacccttga gggcagtgtt ggataaaaat ttatatggaa attttcagtg ttgataatat
    24421 tatattttct ttagggggac aaggggtaag tctgcttgaa tttatttctg tattaacagg
    24481 tcttacttgc gtctttcttg ctacaagatc caaagtggct aatttttggg ttggatatct
    24541 atacaatatt cttctgttca tactttttta tcagaaggga ttatattcga gcatgatagt
    24601 ccagccaata tcttttgcaa tcaatttttt tggtcattat agatggacac atcctcgtaa
    24661 gggagaagaa aatagtaagc atcaattaaa gatatcactc atgtcaaatg gcaaaagatt
    24721 atttttctta ttgctgatcc ttttgcttgg agctgtgtgg ggtactgcac ttacctattt
    24781 caatgacatt tggccgtcca tatttagaga ggcaaggatt ccgtatcttg actctgttat
    24841 aacattcaca attcttacag cccaatatct ttctgctcaa aagagacttg agtgctgggt
    24901 ggcttggttt attgttaata caaccaatat aatactatat atacttgcag gcttggtatt
    24961 tatgccttta gtaagtgcag catatcttgt tattgctttt ttcggatttt caatgtggct
    25021 aaaagagtgg aaaagttaca gtaattaaaa atgaataaaa tattagaaat aaaaggttta
    25081 cgggcctcaa tagagggcaa ggagattttg aaagggatag atttttccgt aaatgaagga
    25141 gaggttcacg ccattatggg gccaaatggc tctggcaaga gtactctttc tgcagttctt
    25201 gctggaagag aaaattttgt tgttactgca ggcgaggtta tttatttagg agaaaatctt
    25261 cttgatcttg ctccagaaga gagagcacat agagggatat ttcttggttt tcaatatcct
    25321 gtggaaatac cgggtgtaag caacgttaat tttatgaaaa cagctattaa tgaaaaaagg
    25381 aaatttgcaa accttcctcc actctctgct gcagaatttc tcaagatgat gagagaaaaa
    25441 atggctgtag ttgagatgga tagctctttt tcgggaagag gagtgaatgt cgggttttcc
    25501 ggaggagaaa aaaagcgaaa tgaaattttt caaatggcaa tgcttgagcc gaaattggct
    25561 attctcgatg agacagacag cggtcttgac gtagatgctc ttagaattgt tgctacaggc
    25621 gtaaataaac ttaaaaggcc tgataatgca tttattatca taacacatta ccaaagattg
    25681 ctcaattata ttgttccgga catagttcat gttctttatg atggtaaaat tatcaagaca
    25741 gcaggcaagg agcttgcgct tgaagttgag aacagaggat atgattggtt gataaacggt
    25801 taaatcatgg cacttcagta taaatacaat cctgcaatcc cggatgtttt tcgctgcaga
    25861 gtaccactac ctggtactca tcaggcgtat ctatcaaatg ggttattgca tgaagaggtg
    25921 agctatgaag ataagcttca tctttcagcg tccagtccga tgggattcga tttaaagatt
    25981 tcccccggag cccagatccc tgagactttg caaataatta gtgtgcgtga aacagggcaa
    26041 acagttgatc ttcagttttc aaatttgctc gattttggag cgggttctga ttccaatgta
    26101 ctgctttgta ctcacactct gtctcttgat gaatttgtta cattatcaga gctaaatatc
    26161 aatatccaac aaggaagcag agtgaatatt gtaataatgc agaacgagca taacgggtca
    26221 tctcataaga tgaattttaa aatcaaacaa gctcaagatt catatctcag attgaatata
    26281 gtttctcttc atggtgccag gctggaaaat aatattgatg tgaatttgga gggagagggt
    26341 gggatctgtg aacttaatgg agtatatctt gtaagtggaa agcaagagat tttaacttat
    26401 gttaacatga gacatgtggt gccaaattgt catagttcac aactatttaa aggcattctt
    26461 gataatgagt cgagagctgt atttagtggt ttaataaatg tagcaaaggg tgctcaaaag
    26521 actgaagcct accaggcaaa tcacaatcta ttgctttcca gaaaggcaaa agtttatgct
    26581 caaccacatc tcgaaattaa tgcagatgac gtgaagtgtt cacacggagc aacaagcgga
    26641 cgtttggacg aattagcttt gttttacatg aggtcgagag ggattggttt agaggaagct
    26701 aaattgttgc aacaacttgc atttatctat gacgtccttg aaaaagtaaa taatgttcct
    26761 ttaagaaatc gcctgcagga actggttgaa tcgaggttaa gaggtgaatt cgaacagtgt
    26821 gcaaattgta gtattaactg ttgttgatta agtgttgata agatttaata ctttcactaa
    26881 ctttctccat cattttgcta tatttttgta aaagtcggta gagcaatctt tcgacttttt
    26941 ctatcatttt cgtataataa ttacaaactt atgaatacat atacctatga agaggcaatg
    27001 gctgccagcc ttgcgtattt taaaggagat gaacttgcag catctgtctg gataaacaaa
    27061 tatgccatga aggattcttt tggcaacctg tacgaaaaaa ctcctgaaga tatgcaatgg
    27121 aggttggcta atgagtttgc cagaattgaa gagaaatatc caaatcccat gactgctcag
    27181 gaaattttcg aacttctcaa ggagtttaag tacattatac ctcaaggggg accaatgaca
    27241 ggaataggta acggttacca gattgcctca ttatcaaact gttttgttat aggacacact
    27301 aaacctgctg attcatatgg cggtataatg cgtattgatg aagagcaggt tcaacttatg
    27361 aagagaagag gaggagtagg gcacgatctt tctcatataa ggccagccgg aagtccggtg
    27421 cttaacagtg ctttgacatc tactggaatt gtccctttta tggaacgtta ttcaaattca
    27481 acgagagaag ttgctcagga tggcaggaga ggtgcattaa tgcttactat ttcgataaag
    27541 catcctgatt cagaggattt cattgatgcc aagcttgacc aaggcaaggt aacaggtgct
    27601 aacatttctg tcaaaattga tgatgaattt atgcgggcgg ctttgcaggg gaaaccttac
    27661 aaacaacagt ttccgattga ttcagacaat ccgaaagtcg taaaggagat tgatgcttca
    27721 gctctttgga aaaagattat tcataatgcc tggcagtctg ccgaaccagg tgtgctgttt
    27781 tgggatacca taataaggga gtcattagct gattgctatg cagacttagg ttataagact
    27841 gtcagtacaa atccttgtgg agaaattcct ctctgtcctt atgattcatg tcgtttgata
    27901 gctatcaacc tttattctta tgttgataaa ccttttacac ctgaagcaaa atttaatttt
    27961 gagcttttca aagatcatgt ccaaaaagca atgcgactca atgatgatct gattgatctt
    28021 gaaatggaga agattgatca gattattgct aaaatcagtg ctgatccgga agaagaagaa
    28081 gttaaagaag ctgagcttaa tctatggaag aaaattcaga aaaaaacatt aggtggaaga
    28141 agaacaggcc tggggataac tgctgaaggt gacatgctcg cagctcttgg tttgatatac
    28201 ggagcggaaa aagctattga ttttgcagta gaagttcaga aaacacttgc cttagaggca
    28261 tacagatcct caaatattat ggcacaggag aggggagcat ttcctatttt tgatgcaagg
    28321 agggaggaaa ataatccttt tatccagaga ataaaaagag aagacgagga gttatacgac
    28381 ctaattgtaa gatatggaag aagaaatatt tcaatgctta ccattgcgcc gacgggtaca
    28441 acatcgctga tgactcagac aacttccgga attgaaccag ttttcatgcc tgtttacaag
    28501 agaagaagga aagtcaatcc taatgacaag aacgctgttg tctcatttaa ggatgaagtg
    28561 ggtgattgct gggaagaata ttatgttttt catcacaagt ttttaacttg gtgccaagta
    28621 aatggttatg acaaagataa ggtaatgtcc atgaaaggtc cacaaattga cgctcttgtt
    28681 gcaaagtcac catattacaa agcaacctct aacgatattg attggctggc caaggttaag
    28741 atgcagggta gaatgcagaa gtgggtagat cactcaataa gtgttactgt taatttacca
    28801 aattccgttt cggaggagct agtcgcagat gtgtacaaaa cagcctggga atatggttgc
    28861 aagggtgtta ctgtatacag ggaaggttca agatcaggcg tacttctttc agcgaaagat
    28921 aaagatacca aaccttcagt tagaaaggtt gaaaggccta aatctcttga ggcagaagta
    28981 ataaggttta aaaatgggcc agagcaatgg attgcacttg ttggacttat gaacggtgct
    29041 ccatatgaaa tatttacagg gttacttgac gaggatacaa gaaatatccc taaatcagtt
    29101 accatggggt atattgtcaa agaaaaagac gaagttacag gtaagtcaag atatgatttc
    29161 cagtatattg accgttacgg ttacagaaat actgtcgggg gtatttcaca tatgttcaac
    29221 aaggaatatt ggaactatgc aaaacttatt tctggtgtac ttcgcaacgg gatgccaata
    29281 gtcgacgtag taaatctcgt tcacggactg caattggaca gtgaggccat aaatacttgg
    29341 aagaacggag ttgaaagaac tttgaaaaga tatattcctg atggcactaa agatgacacg
    29401 ggagcaaaat gtgaaaaatg tggttcatca gaactggtgt atcaggaggg atgtctttgc
    29461 tgcatgaact gtggatatac aaagtgtgga taatgatagt ctatccttgt gcaaaaatca
    29521 atattggtct taagatcact ggtaaacgtt ctgatggtta ccatgatctt gagactttct
    29581 tttttcctgt agaactgaca gatattctcg aagttgttga agcagaggaa actattcttt
    29641 atgtctatgg gaaaaaacca gactgttcta tggaagataa tttgtgttac aaggcttacc
    29701 aacttcttaa aagggatttc aatcttcctc cggttgagat tcacttgttt aagagaatac
    29761 cttatggagc aggacttgga ggaggttctt cagatggctc aaatatgctg atagctctta
    29821 ataaactgtt taatcttggc ctgacagacc atcagttgtg taaatatgca tctaaactcg
    29881 gtagtgattg cccttttttt atttatgctt caaagttgaa ctcaaaggca ggagagggta
    29941 tttatgctga aggcagagga gatattctta ctccaatgat tattcctgat cttgcaaatc
    30001 ataaaatcag actcgatttt tctgatattt atgtatctac tgctgaagct tatgccggtt
    30061 tgaatccgac caggcaaaca gaatcattaa aaaaattact tggctatcca atatcagaat
    30121 ggtcaaagag aattattaac gattttgaag aacctgtatt taacaagtat ccggttcttg
    30181 ctgaaaagaa acagaaattt tatgaggaag gtgcagtcta tgcttctatg tctggaagtg
    30241 gttctgcaat ctatgggata tttgccacat aaatgtaatg aagggcgatt cccttttaaa
    30301 gaaatccgcc cttacgagta ctaaaaccta aacctacaat taatagatgc aataagatcc
    30361 tgtttcgttg catcaacaat tattaagata tctccttgct ctcaataatt tttctaaaga
    30421 gacgttgtgg aagttttgaa gcaaaaagcc cgattaatga aacacccaaa aaagttgcca
    30481 atatgtcgct ccatttaata acaaccgggt aggcttcaat aataaaatta ccgggcatct
    30541 tgataaaacc aaattgttgc tggagcaggc atatgataag gcctgcaaca atcccggcaa
    30601 taattcctgt gaaagagata aggcaccctt cataaaaaaa gatgtttttt atacattttt
    30661 cgtttgctcc caaactcttc agaacgccta catcttctct tttatccata attaacattg
    30721 caagagagcc gaaaaggtta catgaaatga tgattattac gaataaaagt ataagataaa
    30781 ttgagagttt ttcagagccc atcattttat aaagggtttc attttgctcg tatcgatttt
    30841 ttattataaa atcattgccc aataagagag aaaactgagc tttggctttt tcaggattaa
    30901 tcccggcttt aagataaagt tcaacagagg taacttcgcc attatcatat tctatgagat
    30961 ttctggccac atcaatgggg atgtagatat atttatcatc aaactctttt tctatggaaa
    31021 atattccggc aggatatact ctttcgctat tgagagatga aataggatta ataaaggata
    31081 caggcctgaa acgagaagga aagtaaaaat caattccgtt tagaaagaaa accttaagcc
    31141 ctattttctg agcaatacct cttcctacaa tagcttgagg tatctctcca tggtacattg
    31201 agaattcacc ttcgactaca taatttttta aagctgtaac tttttcaaaa gaagaatcga
    31261 ctccttttat tgtagcaatt gcctcttcct gatcatattt aacaaacacg ttttcttcga
    31321 caacttcgca gaagttggcg accattgaag agccttttat ctggtcaaaa gtcaccgact
    31381 tgggcacaaa actcttgcct ttggccggta ctatcaaaag atcacattca tgaacactat
    31441 aaagagaacg gattaacccg tcaaatccat tgtatacaga taatataatg ataagtgcca
    31501 tagttcccaa catgataccc acagcactta ttaatgagat tatgttgatg acattatgcg
    31561 actttttagc aaaaaggtaa tgcctggcta taaaaaatga aaaattcaaa ttattttttc
    31621 agaagttcat caattttttc aacataatcc agagaatcat caataaaata ttccagctca
    31681 gggacaatcc tcaattgctt tgaaactcta ttccccaatt caccgcgtat ggatttagat
    31741 ttggaatcga taattttaaa aacatccttg gcttttgtgg aagggaaaac gcttagataa
    31801 actttcgcta ttgaaaggtc aggactgact ctgacagaag tgacagttac cattgccccg
    31861 tcaaaaaaag ccatgccctt ttcacgaata atttcagcca gatccttctg aatctgtttg
    31921 gccactttaa gttgcctggt gctctctctc tgcatcttct ataataattt aataatgaaa
    31981 tagttcttct tgcctttttg aacgagtata tatttattat ttaaaagctc attttctgta
    32041 atctgaagat ctgtagtttc acatctttct ttattaatag acaatcctcc tgcctgaatc
    32101 atcttacgac attcaccctt agaggagaat atattacaat gaatggataa cagctcaaca
    32161 atatttaacg ggagccttgt tctcgatatc tcaaactgag ggacaccttc aaaaactgaa
    32221 agaaaagttt gttcatcaat gttttttagt ttttcataag ttgaattgcc aaaaaggatc
    32281 tgagaggcat ctaaggcctt ttcatattcc tcatatccat gtaccattac agttacctct
    32341 tttgcaagaa gtttttgcag ttcccgtaag tgaggagcct taagatgatt ttctatggtc
    32401 tcttctatct cctctttagt aagcattgta aatattttta taaatttttg ggcatcttca
    32461 tcactaacat tcagccaaaa ttgatagaat gtatagggag atgtataacg agcatccagc
    32521 caaatattac ctttttcggt tttgccaaac ttacctccgt ctgccttggt aatgagtggg
    32581 caggtaagag caaaagcatc tcctccttcc tttcgnnnnn nnnnnnnnnn nnnnnnnnnn
    32641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32701 nnnnnnnnnn nnnnnaaaaa agaataatct ttcatccagt catagttatt caccataatt
    32761 gctgcattag gagagtcaga tttaaaattg agaaatcttg aaagttgctt ttttatggct
    32821 tcctgattgt atcgtaaagt ctcttcatcc agtaactttc tttctgcaga tttcatggat
    32881 ggatctccta tcattccggt agctcctcct acaagtacta ttggtttgtg accgcaagcc
    32941 tgaaaatgcc tgagcatcat tatacttacc aggtgaccaa tgtgcaaaga atcggcagta
    33001 gggtcaattc ccacgtaacc tgaagcagat tctttattaa gcatctcttc ggtaccaggc
    33061 ataatatcat gtatcattcc gcgccaggta agctcttgta caaagttgtt cattgttgta
    33121 cctctgttat tctatcttta taatttctac aaaaataggg ttttttcttt acttttgcag
    33181 tatgaaaaaa acaataagaa aaatagggat tctgttcatc gcagcaggat ttcttctatg
    33241 tgcatccccc tctactgcta ctgcacagaa tcaatctcaa gatactctta aagagttaat
    33301 ttcatcaatt cccggcatta gtggggttga aagactcaaa acagaccagt ttaaggacaa
    33361 gtatctcctt tttgtagagc agccaataga tcacaaaaat cctgatcttg gaagtttcaa
    33421 gcaaagagtt tttgttatga atgcaggatt agacaaacca actgtagttg taacagaagg
    33481 gtatggaggg gcttatgcaa aaatgcctcg ttatagggaa gaattgtcca gtatatttga
    33541 tgcaaatatt atatttgttg aacatcgcta ttttctcgaa tcaactccag aaccgctcga
    33601 ttggaaatat cttacagcag aaaattcagc aaacgattta cataatgttg taactctttt
    33661 taaaaatatt tataagggta agtggatagc tacagggatt agcaagggtg gtcagacatc
    33721 cttgatatac agagcattct ttcctgagga tgtagacatt actgtttcat atgttgctcc
    33781 attgtgcaga agccgtgaag atggacgtca cgagcccttt attgagaaaa aagttggcac
    33841 atctgagcaa agagcaacta ttctcaattt ccagaaggag attctcaaaa aaagaaatcg
    33901 tcttcaacct aaatttgatt cactgtgttt atccaataaa tatacattca atctgcctct
    33961 taataatatt tacgactatt ctgtattaga gttttcgttt tctttctggc aatggggcaa
    34021 ttctatttat gagatcccgg acagtactgc ttctgacgat caaatcttta aatattggat
    34081 gaaaataagt tctccggatt attttgtcaa taatagttca agtacgcctt tttttgtaca
    34141 ggcagccaga gagcttggtt attacggata tgacacaaaa ccttttaaag aacttttatc
    34201 aattaaaaat gctgaaggct accttactaa actgtttctt ccggaaggat ctgaatttaa
    34261 atttgataaa actctctaca aaaaattgaa aagatttgtt gctacaactg ataaaaagat
    34321 gcttttcata tatggggagt atgatccttg gagtgcggta aaagttaatg aaccaaaaca
    34381 caaaaatgtt gttgtaatgg tgcaaccaaa aggaagccac agatcccgca ta
//
LOCUS       DAOTQL010000003        33133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_1445_length_33133_cov_5.418647, whole genome shotgun sequence.
ACCESSION   DAOTQL010000003 DAOTQL010000000
VERSION     DAOTQL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33133
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_33133_cov_5.418647"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1084)
                     /locus_tag="P5293_00270"
     CDS             complement(86..1084)
                     /locus_tag="P5293_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR48400.1"
                     /translation="MKNAIFVGDTHLGIYKSSDLYHDITLDLFKEIVDTCVRRNIKRI
                     IHFGDFFDNRRHLNIKTLHQGLKIAELLHKAMLEVFIIIGNHDTFYKDKIEPTSLSVF
                     EEHPNIKIIDKVTMLEDAVMVPWGTELKDIPDARYALGHFEINGFPVVQGFEFYKSDI
                     NITNFARFEAVYSGHFHIPSHKNNITYLGAPFQLNFGDAGSSRGYYIFDNDNLEFIQY
                     KDAPQFVIISTDEPIRPEKIRGNIVKLVYKKDYGTSENMKILEQVQIYQPIQLFTDFA
                     RMSSGFISVNGDDTEVMQLKSNRDILMEYIEKMIMPDHIKIATLRQVINGLLKEEI"
     gene            complement(1089..1496)
                     /locus_tag="P5293_00275"
     CDS             complement(1089..1496)
                     /locus_tag="P5293_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRR48401.1"
                     /translation="MSKKDDNYYIKNSDLLAEIVVYKTKGRASEELGKMLLTIAENFS
                     SKGSFAGYTWKKDMISEAVLTCLKYLKNFDEDKSQNAFAYITQICKNAFLTYIKEQNK
                     HSDIKDICYREYMFLVNKPSSSAIDYQSIREKE"
     gene            complement(1565..1927)
                     /locus_tag="P5293_00280"
     CDS             complement(1565..1927)
                     /locus_tag="P5293_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48402.1"
                     /translation="MRLLKHLSHSGLDNKTNIFRALNIKLTDKINAANREIVSEMLEK
                     YKLNKSIIDEVNSLNDELKKNYRGKLKFDFCLEGYEIDKLRTRNEKIRRKYLEISSDL
                     SQLQSYLEKQLSKQKDKS"
     gene            complement(1927..2538)
                     /locus_tag="P5293_00285"
     CDS             complement(1927..2538)
                     /locus_tag="P5293_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48403.1"
                     /translation="MARTHFWHFLQNEEGQPINEAEISVYLANSSTPAYVYLAESGGT
                     ATNVTPQVTTRSDGYFEFWIADALEEYGYTGQKFKIAWSKPGVIDDGYVDYVEISLSV
                     AEVDEYDTDVTKNKTVSNALAKSWNERSKILVTHVIADEWTFLGGNYFVNIVHNFNNE
                     WPLVQCWDVATKQTVEVIAEALTSNAIKIWKIDNNESYITLVG"
     gene            complement(2550..4727)
                     /locus_tag="P5293_00290"
     CDS             complement(2550..4727)
                     /locus_tag="P5293_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48404.1"
                     /translation="MAKFCDSPYFIVEDFIQNIDPYGTAYPKRAHLRGPSSGMVAKLG
                     YGVDLFIKTEAGYLRIKGVPVDDTPTDNVFKFFGPYDNYRTSDVTWTDLLNTYAGSEV
                     YFRKDCQFHDFVNNNSDWKNFVNTNKVYLYFGKIFPDTSENFFNVIFYGMKDYVVDAL
                     PENNRTDRFTEFLQVSFDQQYQEIYNRLKNILTLSDPKETDSDYLYYIGNMFNMTIPN
                     LLGDIPSQRAWVENLPYLLKRVGTYTALNIIWRAIVGGTSNFLNIYERWHPVIDPEDV
                     PYLYFEDYLYTANPIYGGTVAVGPAGEGFYHSLETTGAGGYPTDYSWPDGKILSPHYK
                     VQIDLSGEPLGADYIINKNIMDNLLEYWEEVRPVARVSHYQQLISPLTHFGPAPTSLY
                     GGTFSAFLFTRCTKPLAVGVISATAIYSRSSLSSIWDIHHNLGTEIPIVQCYNPQYER
                     IFPARIETISSNHIRIYWDENNPMNGHCFIAIPEYTQTILGPSAASWNIDHTLPDKYP
                     IVQLRETNFDVFMPLSVTIVDDDTLTATFSSIQAGSIELGKAAYVYHPSGAQDTWVIS
                     HGLDAYGLHIEVYDENDELIIPKNIQINTTSRCTVTFSSAVSGTAVIREIGQTFSDMD
                     GMAADISYLGIGNGDDTRHWDAQYYGALKSLISTHSTTLRGDNNYYYVTSTVPQTSTD
                     LTITEIGLYNLYDDLVWYTACSPIFKPANVELKIFYRIELTIK"
     gene            complement(4727..6436)
                     /locus_tag="P5293_00295"
     CDS             complement(4727..6436)
                     /locus_tag="P5293_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48405.1"
                     /translation="MANTLIPDYLNMDFPTIKARIADLLANTTTFADYNYEGSNITLL
                     IELIAYLGALTTYYTNKVAKNQYIDTADLYETVHMLSRLRGYNPQGYRSAQTDLTITI
                     LTSASISPGDEIHIQRWKQVDAPDLTDENGDILRFSTITDFTYTIPTTATFPYTLTET
                     VPIRQGIVRTYTYRGEDLIDNILYLPFENYDYDDDIDETEHPSVEVQVNDEVWTRVSD
                     FYDELSPLSTVDTVYMLRFDKYEKYLIEFSNNRTVPELTDDIVVYLLKTAGADGAAGA
                     NTITSPETTFLVNRTTGDTIPIAEYTVTNLTATIGSSAPDTIAEIKDASTGAIHSQYR
                     NVTAADYRSHLEARSDIIAAHIWGEQEIAPSGSIQEYNKVHIALIPNEWGDSTISYST
                     SGAEDEIIVPLAYSSSWMETLSTYLEPRKILTTYEQFDLPDLVYFSFDIGIKIKRTYV
                     YTDVMNDVRDKLEYYFNAANRSFAETISHIDIVNYIMDSTESSTTGETWSQVKGIQNL
                     IIRNIDCESYTVYEPNSSGNYPQYVEAASTYPGENQLRKIKLGNNQFPAIYMTNCEFS
                     EET"
     gene            complement(6439..6846)
                     /locus_tag="P5293_00300"
     CDS             complement(6439..6846)
                     /locus_tag="P5293_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HRR48406.1"
                     /translation="MPGKFTYTIKDIYSDFDIEMPRQADGDVKRVTEYDAVKNSLRNI
                     LQTRKGSRRMLPTFGAQLERYLFEPIDEITAREIGNTILQEISSWDSRIEIININVEA
                     DYDNSQYNITVTYSIQGTNYRNVDKVIFVLKRT"
     gene            complement(6852..7145)
                     /locus_tag="P5293_00305"
     CDS             complement(6852..7145)
                     /locus_tag="P5293_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HRR48407.1"
                     /translation="MAKVSRLGDAVTGYCSSGIHGYQDGVIIECSPNVNANGIGVARV
                     GDKVRANCGHIGTIVSGALNTNANGRAIARIGDSFSGTYSGTIVAGSPNVDCE"
     gene            complement(7147..7938)
                     /locus_tag="P5293_00310"
     CDS             complement(7147..7938)
                     /locus_tag="P5293_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HRR48408.1"
                     /translation="MMIKNEFTDMTLPRDKLLGIYRGVVEDNVDPLEAGRVRVRIFGI
                     HTPQKTKTALEGIPTDELLWAEPAYGLIEGSISNYGVWGVPLQGSHVLVFFENGNILR
                     PIYFATLPGGSSSPPTDTLHGFVDPSGTYPDKTGPDFDKGLGIYPHNVVFHVHGGHVI
                     EVDSTPNAKRIRVYHSAGTYIIIDNEGNIEINGVKNRSKTIAENETININGNKNETVS
                     GNVTEGFQSDQQTTVGGNLQITVSGNCTINVTGSCDITGNPINLN"
     gene            complement(7935..9206)
                     /locus_tag="P5293_00315"
     CDS             complement(7935..9206)
                     /locus_tag="P5293_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48409.1"
                     /translation="MIDTSQKPVGRGDFTVAIGTKRGFAFIDARDIIEFTFIEDIFSA
                     SMVGKLVFLDKVGGLELLPIVGHEPIYLSYGLDDDRTQIFMVYSINQISPIGQFEGYD
                     LNQVEMYFVEPLFTTLTQRRYSLSFTNKKISDIIKHISDFMLLCTRPYVNFEETKEII
                     PNFYMPYWTPMEAIKWLSRRASSLESLAPGYLFYNNSRGLNFVTLEKLLKQTELEKGF
                     DGTPQRYYFSTGADQSLNKILGFTISGIDYQSMTGIKGGHKLGYDFVTKSFVDQPYTY
                     ENMIKKFTLFGKKSFFWNISDTSARFDLEGDSDIAFLENMAYSDSFKRYIKEHEVLMT
                     VVGHERRYAGMIVDIIWPSTAKEYITNKSFEGKYLVKSVTHQFSGRTMPFYKQLLVLL
                     KTAYTDSIIKDLYSATKINLEVKTQKLGNVR"
     gene            complement(9203..9583)
                     /locus_tag="P5293_00320"
     CDS             complement(9203..9583)
                     /locus_tag="P5293_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48410.1"
                     /translation="MASIKVTESFEDLTGHTLLNVSYIKLFNIMKEEDGTLFLNIFRS
                     YEINESLFRDVMYYSVYEVQEEDWWELISNGFYNSVGLWWVTPLANKVINPFEELDKE
                     NDIQVLVPSVIPILLREIRDIGEL"
     gene            complement(9602..10723)
                     /locus_tag="P5293_00325"
     CDS             complement(9602..10723)
                     /locus_tag="P5293_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48411.1"
                     /translation="MAGAYKNKQLNEPGLPGWEESGAAVREYSHTKKSITQSSKKDNT
                     PAKQRIRKKIEAQRDAGAKTAPSEEITDDVPAIWCPSSLHTNDTNSLWLKISARGIHS
                     MAAWGRADKTLIPRNKRFTFYFLAPNELQEIIAHDWGPYESLAASIAEKTAWVSRMGT
                     ELRNLKQQLKGTGIETAEEASKFHLYGAGKAFIGAIANAFNKAGDSFPELVRQALIAA
                     SQAEVAKTRVDTALVYKNSERRKYDFVFHLADVGTPLYNEIIYPVRILQYLSSPSKLK
                     SGGVNDLADIDFPFYFTLETYPIDFLKIDYAALTLVEPTWKGPYIGGYPSYCELHLSF
                     VEIPPLWDSTFHDKGLVTVGTKTSKVQIGPQMEGLSRAS"
     gene            complement(10735..13083)
                     /locus_tag="P5293_00330"
     CDS             complement(10735..13083)
                     /locus_tag="P5293_00330"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRR48412.1"
                     /translation="MATSPSIGKEMETFIKEFTKSFATAATKEMLKFKKDIDKTAGMV
                     KGKNVVGGGEGAGTATDRYLKKIHKDILKLYKTDKVEKTKIENKDRKEKRKKDETADE
                     KKRKAELERYYRALRKSMSMREKVERWLLQRFWGRTFLKAWYGFWNLKVLSTIRDGIQ
                     RITGFFESQLHDILGDMAPLFDLAKDTFIFAKNTVMGIGEALWKTAKGIWALAPVKRF
                     FEDIGKKFKDFIFSPFQKFFSSIKKSFFTKFYTLFKQPWMSGKARAELWRQEKKEAIK
                     KWAEEKKNALKHWAYQKALAAKYWLWEKTKGGLGKIISPFKKSAQAIKGGISLMKWAF
                     PLFISAIAGLVSGMMSLLAPLLLPVALGLLAISGASLIKVLIDDPAFKKDWEIFTNWI
                     VEKFKNLFDLVMKAVKEKGVEYGLNIASFGLAVPYRETTKRILKAYEEREKIKREAAE
                     TEELKRIKKDPEYRKKVLEEIRKKNAIQDPKLDEYITTIAKRYEIPEEIFRALIQQES
                     AGGRRNLSKKGAIGMTQLMPKTAKDLDVNPYDPYQNIEGGARYLKQMFNIFGSWEKAL
                     AAYNAGAKRVREYGGIPPYPETQKYVERIKNMAGMAEGGKITGGPSMPGAGIDSKLIR
                     ASVGEWITPKRTAGMLNSILPGGMAALIRDPIGTLMRFASNFTFGGLSAGTLMGFQRG
                     GRVSLTRRELSAPVKGVTGAGLRNLENQEMMLNKLTAALTSGGQREPVALPVPVGMSS
                     ATVNTSPINMMGNIGQGSQPTDPLPTLMSTPVAWYIVLQNTI"
     gene            complement(13293..14126)
                     /locus_tag="P5293_00335"
     CDS             complement(13293..14126)
                     /locus_tag="P5293_00335"
                     /inference="COORDINATES: protein motif:HMM:NF023741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48413.1"
                     /translation="MYEFETVLPGSGKTVKFKPISTGQIKKLLMYENAEDQAVIETAL
                     DDVLAGCVLGEDFKIDDLYLQDRFYLLLEIRKATKGNTYQFQTVCPSCGSQSLQTVNL
                     SEMPVKVLELNKKKVKTPEISASKKKKTQSLQEVKEESMLEEAPPQKDVSEPDIVKIN
                     DNISVRMSLITRGMQKQAMEWVKVNYPEVSDAEKAIHTMTAILALAIQGIITPDGEEK
                     DLSIEDRIYLLDSLTEQEEVKLPKWFEKYDFGVDFSINIKCPQCGYQSKRDVPLEDFF
                     F"
     gene            complement(14164..14913)
                     /locus_tag="P5293_00340"
     CDS             complement(14164..14913)
                     /locus_tag="P5293_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRR48414.1"
                     /translation="MKIPIDIQEYKARFLGGARQYLFYVNLQFPNLSNILKAGVEGAL
                     STAKTVEDFESGARDDLLTKGAIEAGGTAVDVFLLNNSVKKFPYLVKSTSLPDSTIEE
                     IQSHWVGQKYKMASVRSDGDWNVTFIVDNDAKILTKFWAWQKIIHNPQSNIYGKPISY
                     MVDQDIHLLGFDTGSTICCFKLYGAWPKSIGQIQLDYQTNDLVQFDVTFSYQYHTITE
                     REPGALATFLQRAGRSYLDLNMEAIRGKIPI"
     gene            complement(14910..15629)
                     /locus_tag="P5293_00345"
     CDS             complement(14910..15629)
                     /locus_tag="P5293_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48415.1"
                     /translation="MPRILFDVDTYRTRFTGGSRQFLFYILLKLPAGISREMGKTGVL
                     STNDGWKKWFVPAADSALTTFGLGSDFDKWPYLIRSTTIPESMFDEIPIPWQSLDYKI
                     AGYRKYGDWSVDMNVDINGDIIERMYSWQNIIQNSLSELKSGRSIKREHDRANSTQYM
                     QDQEVHMIDYSGNVVKSFVLEKCWPKSVGNITFDYASSELLTMNVVFTFYKMRLETKP
                     STKIADAIKRGYEKLTGIMTI"
     gene            complement(15739..16284)
                     /locus_tag="P5293_00350"
     CDS             complement(15739..16284)
                     /locus_tag="P5293_00350"
                     /inference="COORDINATES: protein motif:HMM:NF018543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRR48416.1"
                     /translation="MPRFDIDSYRASFQGGARQYLFYYKPIFPISIAGGDTEKATYLV
                     RSTNLPETTSDEILTNWQGFDFKFAGKYTYGDWTVTFNVDIDAKIQQMFMQWAQLVHD
                     PTTNIYTAPNLYMADQQLELLGYDGKPIMKYKLFGAWPKSVGTATLDYSANDVVQFDM
                     TFTYIYHVADNVTYGTGVTFG"
     gene            complement(16309..18033)
                     /locus_tag="P5293_00355"
     CDS             complement(16309..18033)
                     /locus_tag="P5293_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HRR48417.1"
                     /translation="MAFYLSPLVDVNEIDLSTTIPAVATSIAAIILRNTYKGPERKKT
                     LITTVNELIDIFGKPTNVANCYQDILAATGYLRYGNALYCTRTMPVSATFAGTKAVSG
                     SEATFTQFVLGSDALTLSDLPSKDPDQFADDVSPTAPWPFYLIANSRGEWGNNIRVAV
                     IDYTTYNEIASGGHSEWEVYSDVSAIDSPLLNSKSFLIIVQVKPQGETSYATKEVWNV
                     STDQDVVDDSGNKLFAETVINESSSYIRISMNESQKDQNITISTSSWQSFGGGADDQD
                     DSVTDALIIDDIDLYANAEEIDINILIDGNKSTTVKQYLVSIAESRKDCIAVLDCLYA
                     DVINNSGNEATSLRTYRQQTLNENTSYASIYGNWIEVYDKWNGKYRWIPCSGHIAGIY
                     ANTDDVSDPWFAPAGLNRALLGNVRRLAWNPTQGERDILYKAGINPLVSFSGQGKVVW
                     GQKTLLDKESAFNRINVRRLFIVLEKAIATAARYFLFEPNDDLTRLLLVNMIDPFLRD
                     VRSRRGIYDYMIVCDETNNTPERIDRNELWCDIYIKPTRAAEFIVLNFIATKTGASFT
                     ELAGATAG"
     gene            complement(18098..18598)
                     /locus_tag="P5293_00360"
     CDS             complement(18098..18598)
                     /locus_tag="P5293_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48418.1"
                     /translation="MAITVNIHRATPSNFRLKFPTLPSETSRAATAELELNLFGTVIP
                     GLSLDQAESPWQGGKLQLQVGKLVFDSWTVNFIVDSEFRNWKALANWIFYINNNKDKF
                     GVLPNNMVVDASLQITDNFSRKIMAINFKNVWIQQLGEITLSTREGGAVLEASATFIY
                     DRYEIG"
     gene            complement(18598..19401)
                     /locus_tag="P5293_00365"
     CDS             complement(18598..19401)
                     /locus_tag="P5293_00365"
                     /inference="COORDINATES: protein motif:HMM:NF028034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail sheath stabilizer and completion protein"
                     /protein_id="HRR48419.1"
                     /translation="MARTYYFYRIFRKTIIQFLDMLADIYVARYDNDGNITKTVLVPV
                     KFGPKEKAYYWIKEYSTEEKLPIISVTLMSIDYDGSRLGNKNENIVISKDYDAKTIET
                     IPNLIPYSFLLNVNIWALHIVDIDQILEQILPFFNPYVFLKINVPEMNADLELKTVFN
                     SATPDIMEEWGEEDWRVLKWTLTFTIQGYFLQPITPTTDPDGKLIEKVISRIYSSEEA
                     MAEETETTFTSGASGMYDEAVYVEALGYDETAGILSRYTVFPEGGKGRT"
     gene            complement(19410..19682)
                     /locus_tag="P5293_00370"
     CDS             complement(19410..19682)
                     /locus_tag="P5293_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48420.1"
                     /translation="MTGKEREEKVAMNKVDKLLEKYLFEEKVKEIPNSMWNNMTKMQI
                     LIKLKSWKMQGYKAVRIHGKILSINTAISDVEEIIKKKQEENPGPA"
     gene            complement(19690..20832)
                     /locus_tag="P5293_00375"
     CDS             complement(19690..20832)
                     /locus_tag="P5293_00375"
                     /inference="COORDINATES: protein motif:HMM:NF039393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48421.1"
                     /translation="MKVKKVNKIKQKYPECDMTTHNQNFYVKAGDVYILIHNSPAVFF
                     GINPENGKFFVSTKAIFNKTPKLAYNYKDVNEYFGHAPGLAAKLKTSLKYLSELGVKT
                     IYQGDLLFTDDKKIENINGMPYITFTPNTITYAIPNDGSDLANRINKAKMGIVVHTEY
                     TGDTLDTLSAKFNIDVSRYFNNPNVWLQDAYFRDESGTVNFSKPEIDRINELISEAER
                     VAKYIKEDIFKKMKSFGLYQLFMIFHNENIKKANALRSVSLYYDDFMIFIKDKYEKQA
                     EQVKSPEAKQKRREMIDSAHHFLTKYKKEIMGLLYLYLIFIRLKDIFVRKFSEISNIG
                     TFIRSGEGYNVAKPEGFCVIDRSGNILKLVDRLEFSRANFTLEKNR"
     gene            complement(20917..21975)
                     /locus_tag="P5293_00380"
     CDS             complement(20917..21975)
                     /locus_tag="P5293_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48422.1"
                     /translation="MRFSNYITEQKSRAVGIFIGRMSPPTKAHQKIIEDSLKKYSKVY
                     IFIIEGEKSSKLAKNFLTYNQRKSILKITNPKAHPILSSHGYIPDIIKQENIETSNGV
                     AIIAGSDRIEDYKKQFIGVDYNVIFDEIKRTAEDISASKVRKAIGENDFEEYKRNVAR
                     GLDNKEWFEKFREFLRATIGRPGNVRGQIFKEQRIYTIQEVLNEDINRHIEHFEDNMF
                     NNGVNGIEKNIELAKLIRDTLSGGSKSKAAITVKWDGCIHPDTIIKTKNGDMKMKEIL
                     GKNDVEVFTYNFDKGIDELNLAIKPRINHNNKNWVEVELENGDKIIVTEDHEFYTINR
                     GWVKAIELNENDNIKETE"
     gene            complement(22042..22362)
                     /locus_tag="P5293_00385"
     CDS             complement(22042..22362)
                     /locus_tag="P5293_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48423.1"
                     /translation="MNKIEEIFKEFNKDRDWLEKGKYLVKTSIKDLEVIRKKIFDTYR
                     DEPDQALKNFDRMVKIKKDVNTKTGKKLGIPDEIIKQMNYEIDMWEKHERSTLMRDLE
                     GMKR"
     gene            complement(22359..22718)
                     /locus_tag="P5293_00390"
     CDS             complement(22359..22718)
                     /locus_tag="P5293_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48424.1"
                     /translation="MKKIDNLIDKYLYRKSDLMALTEAIGMRDLTKFMFKIATAKSID
                     DARRLSSMLGDIDNKLWNEYIKILDKSPDEEQKIQKYVERELKYKGDVRQVPENIRAD
                     IWAITLYLAKTGELKSI"
     gene            complement(22715..22981)
                     /locus_tag="P5293_00395"
     CDS             complement(22715..22981)
                     /locus_tag="P5293_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48425.1"
                     /translation="MKFQSGDYVTGRAQINKKVMLKGVVIREYNNWSVLVAYFDERRR
                     PQYRILFHPQKIEGEPPEALLKKAKENPNWGLNPDERIIQRTNI"
     gene            complement(22982..23344)
                     /locus_tag="P5293_00400"
     CDS             complement(22982..23344)
                     /locus_tag="P5293_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48426.1"
                     /translation="MKRIDEKIERYLSEETYKKQPEWDEIRNLLVKADNEIIKIENDF
                     EKNLEQLSFNAKLVLADEEDDFWFWIRKVGEYDDKDPIFDTLRWFADKLERNPKIKQA
                     VIDKKKKEAFKKYGGGKL"
     gene            complement(23341..23556)
                     /locus_tag="P5293_00405"
     CDS             complement(23341..23556)
                     /locus_tag="P5293_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48427.1"
                     /translation="MDEYAKESCVDLYKGDIEKQSDCWWRFYSRLIGFAQEIWMPYMS
                     KDLIDELVSEMEEHAKFITKKYGWYSK"
     gene            complement(23644..23880)
                     /locus_tag="P5293_00410"
     CDS             complement(23644..23880)
                     /locus_tag="P5293_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48428.1"
                     /translation="MKKIDEKIEKYLIKKMPTDPFVKHQIAMARKTLKMNDVMARIMG
                     GMTKEQARAILKKYGLKEEMKFGKYLNDKENISE"
     gene            complement(24217..24906)
                     /locus_tag="P5293_00415"
     CDS             complement(24217..24906)
                     /locus_tag="P5293_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48429.1"
                     /translation="MNKQNRTGLQKPQWNLYDIEGNQEHMLAESMIMEYTDIAGVKIN
                     YYIRSNDVEYDTLYGEHTGTTYEAPKETKIIYEVVDEPNLWSSFGMTGGDVITAHIPM
                     STWRRDVSKTVSPKIGDAVHIKWYQLANRSFEVCHVDDDDKTFQLKKMIWVLILRPFR
                     YSEQSESAADIAVTSKLISAFGDNTWIEEHANVVDDIDTRIYGYVIAYLTLMGYPLLK
                     MLMNYINNFVI"
     gene            complement(25070..25864)
                     /locus_tag="P5293_00420"
     CDS             complement(25070..25864)
                     /locus_tag="P5293_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48430.1"
                     /translation="MLKPYRAIKKSELIERIKRILGYPVVKVELDDMQIADHIDYARQ
                     KFIKWAVGQGVVEKYFTIALSAGVTDYELPGDVTEVLSYDTRTTGSIHTLFTIENYLY
                     NAGMYDMLLMRGGGSGYTLVSYHIARDFLDTIKRYVFDTYNFTYHRYTNILEIQPPPP
                     SGGTIRWQDTWLDSPGYILVRAYVLEGTEEDLYDNMWIFDYATALSKKTLGRIRSKFA
                     NFTAIGQTGLALDGDTLLSEAQTEIEKLEEQLRNEESWEGLGIFIG"
     gene            complement(25866..26090)
                     /locus_tag="P5293_00425"
     CDS             complement(25866..26090)
                     /locus_tag="P5293_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48431.1"
                     /translation="MDRKMSKLLNDDIGSRHPLAKVPVNIHRVMDILKSLERSYLNYN
                     DKMVFTFIDNAIEMLTAMKKDIEDKIEAGE"
     gene            complement(26075..26479)
                     /locus_tag="P5293_00430"
     CDS             complement(26075..26479)
                     /locus_tag="P5293_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48432.1"
                     /translation="MPIENMSIDKKIEEYLGEVITPESRARAEIRLTYNVIENIIDKE
                     VERAKEILLEAKKKMVFQFRKAIEDVIEEIDKANAEAEGAWGISQNSVDNFFKDMYQK
                     LVREYVMKPKERTSHPIVDAFWEAVDSEWIER"
     gene            complement(26615..29176)
                     /locus_tag="P5293_00435"
     CDS             complement(26615..29176)
                     /locus_tag="P5293_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HRR48433.1"
                     /translation="MPVKYDDFIKRPLQEYSYSPEEVAALSKCSQDIWEFLKYVKIIH
                     PDRGRIAFEPYPYQRKILKILQDERFVIGLWSRQSGKALALDTPLPQPNGKWTTIGDV
                     QVGDFLIGRDGKPTKVVFVTEVMENHNCYEIEFDNGERIVVDEEHLWCVGSSNFGHKN
                     SQDNPRTTHEERVLTTKEIFDKFSNTSHSYYINYHSGVVNDDIELPVDPYILGIWLGE
                     GHLVNGRISTSINDYNEISKIIQEKYLTSSFKKDWRNENGGYFNVYKLMIDLRKINLI
                     NNKHIPKVYFSASINQKLELIRGLMDTNGYCGKDGVCEFSQKSSTLIEDIREIFSSIG
                     IKTKLNYRVINGERYHSLVFSTTRFSVFNLSRKKDRQTKCFGHDKNTRIYIKNIIKVN
                     SVPVKCIQVDNDEHLFLCGKTMIPTHNTTTVCAYALWYAIFNPDKIVGIASNKQISAI
                     DILSRLKIMYEELPVWLKPGVQEYSKTFVNFDNGSKIMVSATSPDSFRGRTINLLILD
                     EFAFVQKNKAEEFWSSNLPTIAASSESRVVIISTPNGMFNLYHRLYTQAERKENTFVA
                     IKSTWKDVPGRDRKWASDQRKNLGKQKFIQEYECEFLGSTNTVIDSDIIEKLYDKCTR
                     PIVEQANGKLKIYEKPVEKAVYVIGVDTAKGTGENYSTMQVLKIVQVKPIILQQVATF
                     NDNYIDVYTFADIVNRTSIYYNSAFIMAENNAEGSTVVTRLWWEYENPGLVNSGSKTK
                     DLGVRADRRTKPRAVMLMKKLVEDGSLTIVDYDTVDQISTFIEKEGKFFGKDMPDDLI
                     TALYWGCYFTTTDMLEESFEIKKTEIEDDVWGVLSDIEPIAEDWSWLSRTGKLIN"
     gene            complement(29244..30836)
                     /locus_tag="P5293_00440"
     CDS             complement(29244..30836)
                     /locus_tag="P5293_00440"
                     /inference="COORDINATES: protein motif:HMM:NF016201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HRR48434.1"
                     /translation="MKQNDLQRLIKIEDRIKQYVEEYGMKCLPVEFDIVPPQKMIEIL
                     AYRMPTNISNWKYGRDYERLKTVFDTVDPNLPLELVVHDNPSRAFLMNSNTLAVQVLV
                     MAHVYAHVNFFTESKWYVNFREDIVEVLAAAQKRFNDYERIYGLDEVEKTEDAGHALQ
                     WHSSPFNQETEMERKKRVFEQMKKKKLPSRAVYGDIVRDTKKDVAEDYDVYNNRLWRK
                     ILATTPIEPTEDILRFIIDNSRILEDWQKDILEILRMEGQYFWPYSKTKYMNEGWATY
                     WHNIFMNRLFDEGLLTAEEHGQYNYSNSLVKATNKMGMNPYLVGSEMWKEIEERWNKG
                     RYGTDYDNCTDAKTKENWDTKEMKGREKMFEVMRSYTDWFFMQDFLTVDLIDKLDLYI
                     YQIMETVASIDYVRTNHTVEQVRELIINSFANSGIPKIEIVNGNLDKGGHLELVHRYN
                     GIPLHKKYAQETLKHIYYLWGRPVTLKTFTRDKKTGKDKELYYTEDGTVSPPASSENM
                     EASDNPFSTFHFDMMDLAPISL"
     gene            complement(30836..32155)
                     /locus_tag="P5293_00445"
     CDS             complement(30836..32155)
                     /locus_tag="P5293_00445"
                     /inference="COORDINATES: protein motif:HMM:NF016195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HRR48435.1"
                     /translation="MIISHENWDLSSRGRKDAARHREKIEDTIRKNIKDIIAEESIIT
                     QKHGKTVRIPVKGLRDYRFIYGSTGTAGGVGQGKGKAGDIIGRRSKGKKNDDSKPGNA
                     RGDDYMETEVDIDYLINIMFQDLGLPYIEEKTKAELLVPSGWKFETVSKVGIHPRIHK
                     RRTIKESIKRMAIYAAEIMDETKCDEETAYKALATSHGDLVDAINLIKENKVNPDADV
                     QSLFIEDDDLRFKQMEQEFEIHSNAVVIAMMDTSGSMTPEKKYLARSMLFWMVEFLKK
                     TYNHVQIRFIAHHTIASLVDEETFFKKGESGGTYCHTAFDLARDLVEREYPVNQWNVY
                     VVYVSDGEDFNPDETMQSTKLLLDKKINMLGYCEIHISSQGYFHASHGNLMEKYTNTF
                     DFKIETEAGTNFYKDNERHFLACIIKGKEHIYPALKHMLFEKKRNKK"
     gene            complement(32372..>33133)
                     /locus_tag="P5293_00450"
     CDS             complement(32372..>33133)
                     /locus_tag="P5293_00450"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48436.1"
                     /translation="KKQEIDVKALRLEGKEQGEGMRGISPRFIINALNIALGQKEEKK
                     CVNPIDIIRALRSNFEHQIGITEEESKKYLTLLLGEKESVSAEFKDIAKKEVNMAFLS
                     AYEEQAQSLFENYIRNADAFCRKEKIQDSITGEYSDPDEKLMRQIEEYIGIPINSKTE
                     FRNGIFVYKSAQLEKGEAFTFKTYDPLRIAIEKKLMSDLKNVVSLTLADKSATDKRTQ
                     MKRKSAVTNLTKRGYCPECANILLSFVGEILRKEE"
BASE COUNT         9383 a         6899 c         5018 g        11833 t
ORIGIN      
        1 tttctgtaat cgaaacagag gctcaaatca tcttctttta cattctgtaa cagataataa
       61 ttattatcag aatttcttat tatcattata tctcctcctt gagtagacca tttattactt
      121 gtctcaatgt ggcaattttt atatggtctg gcataatcat tttctctata tactccatca
      181 atatatccct gttgcttttc agttgcatga cttccgtatc atccccgttt acacttataa
      241 aaccggatga cattcttgca aagtccgtga acagttgaat gggttgatat atctggactt
      301 gctccaatat cttcatattt tcggaagttc cataatcttt cttgtagacc agttttacta
      361 tattacctct tatcttttct ggtcttatag gttcatctgt agaaataatc acaaactgtg
      421 gagcatcttt gtattgtata aattccaaat tatcgttatc aaaaatataa tatcctctac
      481 tactaccagc atccccaaaa tttaattgga atggggctcc aagataggtg atgttgtttt
      541 tgtgtgatgg gatgtgaaag tgtcctgaat agactgcctc gaatctggca aagttggtta
      601 tgtttatatc tgatttataa aactcaaatc cctgaaccac tggaaaacca ttgatttcaa
      661 aatgaccaag ggcatatcga gcatcaggaa tgtccttcaa ctctgttccc catggcacca
      721 ttactgcatc ttccaacatt gtgactttat caattatctt gatattcgga tgctcctcaa
      781 atacacttag gcttgtgggt tcgatcttat ccttgtagaa tgtatcgtga tttccgataa
      841 tgatgaaaac ttccagcatt gctttgtgga gaagttcggc aattttcaat ccctgatgaa
      901 gagtttttat attgagatgt cttcgattat cgaagaaatc tccgaaatga ataatacgtt
      961 ttatatttcg tcttacacag gtatcaacaa tttctttgaa cagatcaagt gtaatatcgt
     1021 gatagaggtc cgagctttta taaatgccta aatgtgtatc acccacaaaa attgcatttt
     1081 tcattacatc attccttttc acggatggat tggtagtcaa tcgcactact ggatggctta
     1141 ttaactaaaa acatatattc acggtaacag atatccttta tgtcactatg cttattctgc
     1201 tcttttatgt atgttagaaa agcattttta caaatttgtg tgatataagc aaaggcattt
     1261 tgtgatttgt cctcatcgaa atttttgaga tactttaaac aagtcagaac cgcctctgaa
     1321 atcatatctt ttttccaagt ataaccggca aaactgccct tggatgaaaa gttttccgca
     1381 atggtaagta acattttacc aagttcttca gatgctcttc cttttgtttt ataaaccaca
     1441 atttctgcta ataaatcact gtttttgatg taatagttat catccttttt tgacataatc
     1501 tcctcattat tatgatatgt attttacgct aaaataggga cgaaggaaac ctatatttga
     1561 aaagttatga tttatctttt tgttttgaga gttgtttttc caagtatgat tgtagctggc
     1621 tcaaatcgct tgatatttca agatattttc ttctgatttt ttcgtttctt gttcgcagct
     1681 tgtctatttc atatccttca aggcaaaagt caaatttcaa ttttccccgg tagttttttt
     1741 tcagctcgtc attgagagag ttcacttcat caataatact tttattgagt ttgtatttct
     1801 ctaacatttc agatactatc tcgcgattgg ctgcattaat tttatccgtc aatttgatat
     1861 taagcgctcg aaatatattg gtcttattgt caagaccgga atgtgataaa tgttttaata
     1921 atctcattat ccaacaagag taatataact ttcgttatta tcaatcttcc agatttttat
     1981 agcgtttgat gtcagggcct cggcaattac ctcaactgtt tgttttgttg caacatccca
     2041 gcattgaaca agtggccatt cattattgaa attatgcact atattgacaa aatagttacc
     2101 cccaagaaat gtccattcat cagctataac gtgagttacc aatatttttg atctctcgtt
     2161 ccagcttttc gcaagtgcgt tgctcaccgt tttgtttttg gtaacatctg tatcatattc
     2221 atcgacctct gccacggaaa gagaaatttc aacatagtca acatatccat catcaattac
     2281 accgggcttt gaccacgcga ttttgaactt ttgccctgta tatccatact cttccaaggc
     2341 atccgcaatc caaaactcaa aatacccatc agaccttgtt gtaacttgtg gtgtgacatt
     2401 tgttgctgtt ccaccacttt ctgcaagata tacatatgca ggtgtggatg aatttgccag
     2461 atagacggaa atctccgctt cgtttattgg ttgaccttcc tcattctgga ggaaatgcca
     2521 aaagtgtgtt cttgccataa taatacctct tatttaattg ttaattctat tctgtaaaat
     2581 attttcaatt caacattagc cggtttgaaa ataggactac aagccgtgta ccataccaaa
     2641 tcatcataca agttatataa gccaatttcg gtgattgtca aatcagttga tgtttgtggc
     2701 acagtagatg taacataata ataattatta tcacctctca atgtcgtcga atgtgtacta
     2761 attaggcttt tcaaagcccc ataatactgt gcatcccaat gtcttgtatc atccccatta
     2821 ccaataccga gataggaaat gtctgccgcc attccatcca tatcggaaaa tgtctgccca
     2881 atttctctta taacagctgt tcctgaaaca gccgatgaaa atgttaccgt gcatctactg
     2941 gtagtattta tttgtatatt tttaggaata attaattcat cattttcatc atacacttcg
     3001 atatgaagtc catatgcatc aagaccatgt gaaataaccc aagtatcttg cgcacctgac
     3061 ggatgataaa catatgctgc cttaccaagt tcaatacttc cagcttgaat ggacgagaaa
     3121 gttgctgtaa gtgtatcatc atcaactatc gtgacagata aaggcataaa cacatcaaaa
     3181 tttgtttccc ttaattgaac aataggatat ttatctggta gtgtatggtc tatattccat
     3241 gaagctgctg acggcccaag aattgtttgt gtgtattccg gtatggcgat aaaacaatgc
     3301 ccgttcattg gattgttctc atcccaatat atcctgatat gattggaaga aattgtttct
     3361 attcttgctg gaaaaattct ctcatattga ggattatagc attggactat aggtatttca
     3421 gttcccaaat tgtggtgaat atcccaaatt gatgatagag aacttctact gtaaattgcg
     3481 gttgcactta ttacaccaac tgccaatggt tttgtacatc gtgtgaatag aaatgcagaa
     3541 aatgtcccac catagagaga tgttggagcg ggcccgaaat gtgtcaaagg agaaatcagt
     3601 tgttgataat gtgatactct tgcaactggg cggacttctt cccaatactc aagaagattg
     3661 tccataatgt ttttattgat gatataatca gcaccaagag gctcaccgct caaatctatc
     3721 tggactttgt aatgtggaga aagaatttta ccatcaggcc aagaataatc tgtcggatat
     3781 cctccagccc ctgtggtttc caagctatga taaaatcctt ctccagcagg ccccaccgcc
     3841 acagttccac cataaatcgg attggctgta tagagataat cctcaaaata tagataggga
     3901 acatcttctg gatcaataac tggatgccat ctttcatata tattcaagaa attactcgta
     3961 ccgcctacaa ttgctctcca aataatattg agtgctgtat atgttcccac cctcttgaga
     4021 agataaggta agttttcaac ccacgccctc tgtgaaggaa tatcaccgag cagattagga
     4081 atggtcatat taaacatatt cccgatatag tacagataat cactatctgt ttccttcgga
     4141 tcagaaaggg taagaatatt ttttaggcga ttgtaaattt cttgatattg ctggtcaaag
     4201 gagacctgta gaaattctgt aaatctgtct gtcctgttat tttccggtag ggcgtccacc
     4261 acataatctt tcataccgta gaaaataaca ttgaaaaagt tttcacttgt atctgggaaa
     4321 atttttccaa aataaagata taccttgttt gtattgacaa aatttttcca atctgaattg
     4381 ttatttacaa aatcgtgaaa ctgacaatct ttcctgaaat aaacttctga acccgcatat
     4441 gtattcaata aatccgtcca agtaacatct gatgtcctgt aattatcata tggcccgaaa
     4501 aacttgaaaa cattatccgt gggtgtatca tcaacaggaa caccttttat tctcaaatat
     4561 cctgcctcgg tctttataaa caaatcaaca ccataaccaa gtttagcaac cattccggaa
     4621 cttggccccc tcaagtgagc ccgctttgga tatgctgttc catagggatc aatattctgg
     4681 ataaaatctt caactataaa ataaggagaa tcgcaaaatt tagccattat gtttcctctg
     4741 aaaattcgca attggtcata taaattgctg gaaattgatt gttcccaagt tttatctttc
     4801 ttagttggtt ctcgcctgga tatgttgatg ctgcttctac atattgagga tagttaccgc
     4861 tactatttgg ttcgtataca gtataacttt cacaatcaat atttcttata atcaaattct
     4921 gtattccttt cacttgagac caagtttctc ccgtggtgga tgattctgtt gaatccataa
     4981 tatagtttac aatatcaatg tgagaaattg tttctgcaaa tgaacggttt gcggcattga
     5041 agtagtattc aagtttatct ctcacatcat tcattacatc tgtataaaca tatgttcttt
     5101 ttatcttaat acctatatca aatgaaaaat atacaagatc aggtaaatca aactgctcat
     5161 aggttgtaag tatttttctt ggttcaagat atgtagatag cgtttccatc caagatgatg
     5221 agtatgctaa tggaacaatg atttcatctt ctgctccact tgttgaatat gatatagtcg
     5281 aatctcccca ctcattaggt atcagagcga tgtggacttt gttatattcc tgaatgcttc
     5341 ctgacggagc aatctcctgt tcaccccaaa tgtgcgctgc aataatatca gaccttgcct
     5401 caagatgtga tctataatct gccgctgtaa cattcctgta ctgtgagtga atagccccag
     5461 ttgatgcgtc cttgatttct gctatggtat caggagcaga tgaacctata gtggcggtta
     5521 aatttgtcac agtatattct gcaattggaa ttgtatctcc agttgttctg tttaccagaa
     5581 atgtcgtttc tggagatgta attgtatttg caccggccgc cccgtcagct ccggcagttt
     5641 tcaagagata aacaactata tcatctgtaa gctcgggaac agttctatta ttggaaaatt
     5701 ctatcagata cttttcatac ttgtcaaatc tcaacatata aactgtatca accgttgata
     5761 atggtgataa ctcatcataa aaatcagaaa cccttgtcca gacttcatca tttacttgaa
     5821 cttctactga aggatgttct gtttcatcaa tatcatcatc ataatcataa ttttcaaatg
     5881 gaagataaag aatgttatca atcaaatctt ctcctctata tgtgtatgtt cttacaattc
     5941 cctgtcttat cggaactgtt tctgtgagtg tatatggaaa ggttgcagtt gtaggaattg
     6001 tataagtaaa atcggtgata gttgaaaatc tcaaaatatc tccattctca tctgtaagat
     6061 ccggcgcatc aacttgtttc catctctgaa tatggatttc atcgccggga gaaatacttg
     6121 cacttgtaag aattgtaatt gttaaatctg tctgtgctga cctatatccc tgtgggttat
     6181 atcctctcaa ccttgatagc atatgaactg tttcatacaa gtcggctgtg tctatatatt
     6241 gattttttgc aaccttattt gtataataag ttgtaagcgc cccaagatat gcaatcagtt
     6301 ctataagaag agtgatatta cttccctcat aattataatc agcaaaggta gttgtgtttg
     6361 caagtaaatc tgctatcctt gcttttatag tgggaaaatc catatttaga taatctggaa
     6421 ttaatgtatt tgccataatt atgtcctctt caaaacaaaa attactttgt caacatttcg
     6481 ataatttgtt ccttgtatcg aatatgttac tgttatatta tactgtgaat tatcataatc
     6541 cgcttccaca ttgatgttta ttatttcaat gcgggaatcc cagctggata tttcctgtaa
     6601 tattgtattg ccaatctctc tagcagttat ctcatctatc ggttcaaata aatatctttc
     6661 aagctgtgcg ccaaatgttg gcaacattct cctgcttccc tttctcgtct gtagaatatt
     6721 tcgaagggaa tttttaacag catcatattc agtaaccctt ttaacatccc catctgcctg
     6781 gcggggcatc tcaatatcaa aatctgaata gatatcttta attgtatatg taaactttcc
     6841 tggcattgat cttattcaca atcaacatta ggcgatcctg caactatagt tccactatat
     6901 gttccagaaa aagaatcacc tattcttgcg attgccctcc cattggcatt tgtgtttagt
     6961 gctcctgaaa ctatcgttcc aatatgacca caattggctc ttactttatc acctactctt
     7021 gccacaccta taccatttgc atttacattt ggcgaacatt ctataataac accatcttga
     7081 taaccgtgta tgcctgatga acaatatcct gtaactgcat ctcccaatct acttacttta
     7141 gccatatcaa ttcaaattta tcggatttcc tgtaatatca caggaacctg taacatttat
     7201 tgtacaattc ccacttactg taatctgaag atttccacca actgttgtct gctggtctga
     7261 ttgaaatcct tctgtcacat ttccagaaac ggtttcattc ttattaccat ttatatttat
     7321 ggtttcattt tccgcaatag tcttgcttct attttttaca ccgttgattt ctatatttcc
     7381 ctcattatct atgatgatat atgtaccggc agaatgatat actctaattc tttttgcatt
     7441 tggtgtcgaa tcaacctcta taacatgacc accgtgaaca tggaaaacaa cattatgtgg
     7501 gtatattccc aatcccttat caaagtcagg ccccgtttta tcgggatatg ttcccgatgg
     7561 atcaacaaat ccatgaagag tgtcagttgg tggtgatgac gatccgccag gtaatgtagc
     7621 aaaatatatt ggtcgaagaa tatttccatt ttcgaaaaat acaagaacat gagaaccctg
     7681 aagtggaacg ccccacactc cataattcga aatagagcct tcaattaatc catatgccgg
     7741 ttccgcccag agtagttcat ctgtaggaat accttccaag gcggtttttg ttttttgtgg
     7801 agtatgaata ccaaatattc tcactcttac tcttcctgct tccagaggat caacattatc
     7861 ctcaacaacc ccgcgataaa tccctaataa tttatctctg ggtagagtca tatctgtaaa
     7921 ttcatttttt atcatcatct tacattaccc aatttttgtg tttttacttc aagatttatt
     7981 tttgtagcac tatataagtc ttttattata gaatctgtat atgccgtttt caataaaaca
     8041 agaagttgtt tatagaaagg cattgtcctt ccgctaaatt gatgagtgac agattttaca
     8101 agatactttc cttcaaatga tttattagta atatattctt ttgcagtact tggccatatg
     8161 atatccacaa tcattccggc gtatcttctc tcatgcccta ctactgtcat taaaacttca
     8221 tgttccttga tgtatctctt gaatgaatca ctatatgcca tattttccaa aaaagcaata
     8281 tcactatcgc cttcaagatc aaatcttgcc gatgtatcac taatattcca aaaaaaactt
     8341 ttctttccaa ataatgtaaa ttttttaatc atattttcgt atgtataagg ttgatcaacg
     8401 aagcttttag tgacaaaatc atatccaagt ttgtggcctc cttttatacc tgtcattgat
     8461 tgatagtcaa ttccacttat tgtaaatcca agtattttat ttaaactctg atctgcccca
     8521 gtagagaaat aatatctttg cggggttcca tcaaaaccct tttccaattc ggtctgtttc
     8581 aataattttt ccaatgttac aaaatttaat cccctcgaat tattataaaa tagatagcct
     8641 ggcgccagag attccaaact acttgctctt cttgaaagcc attttatagc ttccataggc
     8701 gtccagtatg gcatataaaa attaggtatg atttcttttg tctcctcaaa atttacatat
     8761 ggccgagtac aaagtaacat aaaatctgaa atatgtttta taatatcaga aattttctta
     8821 ttagtgaaag acaagctata tcttctttgc gtaagagttg taaacagtgg ttcaacaaaa
     8881 tacatttcca cttgattcaa atcatacccc tcaaattgcc caattggaga aatttgattt
     8941 atagaataaa ccatgaatat ttgtgttcta tcatcatcca acccatatga taaataaatt
     9001 ggttcgtggc ccacaatagg taaaagttca agaccaccaa ctttatcaag aaaaactaac
     9061 ttaccaacca tgcttgcaga aaaaatatct tcaataaaag taaactctat aatatctcgc
     9121 gcatcaataa acgcaaagcc ccttttagtt ccaatagcaa cagtaaaatc ccctctacca
     9181 acgggttttt gagatgtatc tatcatagtt ctccaatatc tctaatttca cgcaaaagaa
     9241 ttggtattac acttggaaca agaacttgta tatcattttc tttgtccagt tcttcaaatg
     9301 gatttataac tttatttgcc aagggtgtaa cccaccataa accaactgaa ttataaaaac
     9361 cattggaaat taattcccac caatcttctt cttgaacctc ataaacagaa tagtacataa
     9421 catctctaaa gagagattca tttatttcat atgatcgaaa aatatttagg aacaatgttc
     9481 catcttcttc cttcataata ttgaaaagtt tgatataaga aacatttaat aatgtatgac
     9541 cggttaaatc ttcaaatgat tctgtgactt ttattgatgc catgaattaa aacacctccg
     9601 cttaggatgc tctgcttaat ccctccattt gaggtccgat ctgaactttt gatgttttcg
     9661 ttccaactgt taccaaacct ttatcatgga aagtgctatc ccataatgga ggtatttcta
     9721 caaatgaaag atgtaattca caatatgaag gatatcctcc tatatatgga cccttccaag
     9781 ttggttcaac aagtgttaat gctgcataat ctatttttaa aaaatctatg ggatatgtct
     9841 ctaatgtaaa ataaaatgga aaatctatat ctgctaaatc atttacccct cctgatttta
     9901 gtttggatgg agatgataaa tattgcagaa tccttacagg ataaatgatt tcattatata
     9961 aaggtgtacc aacatccgca agatgaaaaa caaaatcgta ttttcttctt tcagaatttt
    10021 tataaaccag cgcggtatca actcttgttt tcgcaacttc ggcttgagat gcggctatga
    10081 gagcttgtct gacaagctct ggaaaactat cgccggcttt attaaatgca ttagcaattg
    10141 ccccaataaa tgctttaccg gctccatata agtgaaattt tgaagcctcc tcagccgttt
    10201 caatacctgt cccctttagc tgttgtttaa ggtttcgaag ttctgttccc attcgtgata
    10261 cccatgcggt tttttcagca atacttgcag ctaaggattc atatgggccc caatcatgag
    10321 caattatttc ctgtaattcg ttaggcgcaa gaaaataaaa tgtaaatctt ttatttcggg
    10381 gaataagtgt tttatctgcc ctgccccatg ctgccatact atgaatacca cgtgcagata
    10441 tttttaacca aagactattt gtatcattag tatgtaatga tgaagggcac catattgccg
    10501 gaacatcgtc tgtgatttcc tctgatggtg ctgtttttgc accggcatca cgctgggctt
    10561 ctattttctt tcttattctc tgcttggccg gtgtattatc ctttttactg ctctgtgtaa
    10621 tacttttttt tgtgtgagaa tattccctaa ctgcagcacc agattcttcc cagccaggta
    10681 agccgggctc atttaattgt ttatttttat aagctcccgc cattttgttt ctccttaaat
    10741 tgtattttgt aaaacaatat accaagccac aggtgttgac attaaagtag gtagtgggtc
    10801 tgtgggttgt gatccttgcc ctatattacc catcatatta ataggggatg tatttacagt
    10861 tgcagaactc ataccaactg gaacaggaag agccacgggt tccctttgtc ctccactcgt
    10921 aagtgcagca gtaagtttat ttaacatcat ttcttgattt tctaaatttc tcaatcctgc
    10981 tcctgttact cccttaacag gtgcactgag ttctcttctt gtaagagaca ctctaccgcc
    11041 cctctgaaat cccattaacg tgccagcgct caaaccacca aaagtaaaat tacttgcaaa
    11101 tctcatcaaa gttccaattg gatcacgaat taaggcagcc attccaccag gcaaaatgct
    11161 gttaagcatt ccagcagttc ttttcggtgt tatccattca ccaactgatg ccctaataag
    11221 tttactatca atgccggctc cgggcattga aggcccgcct gtaatttttc caccttccgc
    11281 catcccggcc atatttttaa ttctttcaac atatttttgt gtttctggat aaggaggaat
    11341 acctccatat tctcgtactc gttttgcccc ggcgttatat gcggcaagag ccttttccca
    11401 gcttccaaat atattaaaca tctgtttaag atatcttgct ccgccttcaa tattttgata
    11461 tggatcataa gggttcacat ccagatcttt cgccgttttt ggcattaatt gtgtcattcc
    11521 tattgcgcct tttttggaaa gattacgtct acccccggcc gattcttgct gtatcagagc
    11581 tctaaaaatt tcttctggta tttcatatct ctttgctatc gtagtaatat attcatcaag
    11641 ttttgggtct tgtatagcat ttttctttct tatctcctcg agcacttttt ttcgatactc
    11701 tggatctttt tttatgcgtt tgagctcttc agtttctgca gcctcgcgtt ttattttttc
    11761 cctttcttca taagccttca atatcctctt tgttgtttct ctgtatggca cggccagacc
    11821 aaaactggct atattcaatc catattcgac ccctttttct ttaaccgctt tcataacaag
    11881 atcaaataaa tttttaaatt tttcaacgat ccaattagta aaaatctccc aatctttttt
    11941 aaatgctgga tcatcaataa gaacttttat caatgatgcg ccactaatag ccaaaagacc
    12001 aagagcaact ggaagcaata gaggagccaa taatgacatc attccactta ccagaccagc
    12061 aatagccgaa ataaatagtg gaaaagccca tttcatcaat gaaataccgc cttttatagc
    12121 ttgagcagat tttttaaatg gtgagattat tttgccaagg ccgccttttg ttttttccca
    12181 taaccaatac tttgcagcca gagctttttg atatgcccaa tgttttaatg cattcttttt
    12241 ttcctcggcc cattttttaa ttgcttcctt tttttcctgt ctccatagct ctgcacgagc
    12301 cttgccagac atccatggct gtttaaataa tgtataaaat tttgtaaaaa aacttttctt
    12361 tatagatgaa aagaacttct gaaacgggga gaatataaaa tctttaaatt ttttacctat
    12421 atcttcaaaa aatctcttaa ctggggccag ggcccagata cctttcgccg ttttccagag
    12481 agcctctcct atacccatta cggtattttt tgcaaatata aaggtatctt ttgccaaatc
    12541 aaagagaggc gccatatcac caagtatgtc atgtaactgt gattcgaaaa accctgttat
    12601 tctttgaata ccatctctta ttgtcgaaag aactttaaga ttccagaaac cataccacgc
    12661 tttcaagaat gttctacccc aaaatctttg taagagccac ctctcgactt tctccctcat
    12721 tgacattgat tttcgaaggg ccctgtaata tctctctaat tctgcttttc ttttcttttc
    12781 atccgctgtt tcatcttttt tacgtttttc ttttctatct ttattttcga ttttggtttt
    12841 ttctactttg tctgtcttat aaagttttaa aatatctttg tgaatttttt taagatatct
    12901 atcagtagca gtaccagccc cctcgccacc tccaacaaca tttttacctt taaccatacc
    12961 agcagtttta tcaatgtctt tcttaaactt caacatttcc ttagttgctg ctgtggcaaa
    13021 agatttggta aattccttga tgaaggtttc catttctttt ccaattgagg gactggttgc
    13081 catatattac ttcctctatt tctttatttt ttctggttgc ttcatagcat ctgctttttg
    13141 tttcatatct tttataacaa ggttgagaaa ggcttctctt tcaaaatcag gcaagagatt
    13201 gctttcctca atacttatat tcgcccggct tgctaaataa tactgctcct ctgtaatagt
    13261 gtgcaagctc gtgccacaac acaatgtata gattaaaaga aaaaatcttc aagaggaaca
    13321 tccctctttg attgatagcc acattgagga catttaatgt taattgaaaa atcaacacca
    13381 aaatcatact tctcaaacca ctttggaagt tttacctctt cctgttctgt aagggaatcc
    13441 agtaaataaa ttctatcctc tatgctcaag tctttctctt caccatctgg agtaataata
    13501 ccttgtattg caagagcaag aattgctgtc attgtatgaa tagccttttc agcatcagat
    13561 acttctggat aattaacttt tacccattcc atcgcctgtt tttgcatacc tcttgtaatt
    13621 aaggacattc tcacagatat attatcattt attttaacaa tatcgggctc tgacacatct
    13681 ttctgtggtg gcgcttcctc taacatactc tcttctttta cttcttgaag tgattgtgtc
    13741 tttttctttt tagatgctga aatctctggt gtcttaacct tctttttatt gagctctaaa
    13801 actttcaccg gcatttcaga gagattcacc gtctgtagtg attgagagcc acaacttggg
    13861 catactgttt gaaactgata agtatttcct tttgttgcct ttcttatttc caataataaa
    13921 taaaatctat cctgtaaata taaatcatct attttaaaat cttctcccaa aacacatccg
    13981 gctaaaacat catcaagtgc agtctcaatt actgcttgat cttctgcgtt ctcatacatt
    14041 agcagctttt taatctgccc cgttgagatt ggtttgaatt ttacagtttt accacttcct
    14101 ggcaaaacag tttcaaattc atacacattg atataccttt taaaatttga cataaaaacc
    14161 tccttatatt ggtatctttc ctcttattgc ttccatattc aaatccaaat aacttcttcc
    14221 tgccctctgt aagaatgttg caagagcgcc aggttctctt tcggttattg tatgatattg
    14281 ataggaaaaa gtaacatcaa attggactaa atcatttgtt tgatagtcta attgaatttg
    14341 tcctatgctt ttcggccagg ccccataaag tttaaaacaa caaatggtgc tccctgtatc
    14401 aaaacccaaa agatgaatat cttgatcaac catataggaa atgggcttac catagatatt
    14461 actttgtgga ttatgaatta ttttttgcca cgcccagaat tttgtaagaa tcttggcatc
    14521 attgtcaaca ataaatgtaa cattccaatc accatcactt cgaacagagg ccatcttgta
    14581 tttttgtcca acccagtgtg attgtatttc ttcaattgta gaatcgggta aagatgtgga
    14641 tttaacaaga taaggaaatt tttttacact attattcaat agaaaaacat caaccgctgt
    14701 acctccagcc tcaattgccc cctttgttaa aagatcatct ctggcgccgc tttcgaaatc
    14761 ctcaactgtt tttgctgttg ataatgcacc ctctactccc gcttttaata tattactgag
    14821 atttgggaat tgaagattta cataaaacag atattgtctc gcgccaccga gaaatcttgc
    14881 tttatactct tgaatatcaa taggtatttt catatcgtca ttatccccgt tagtttttca
    14941 tatcctctct ttattgcgtc cgctattttt gtagatggtt ttgtctctaa tctcatttta
    15001 taaaatgtaa aaactacatt cattgttaag agttcggacg aggcataatc aaaagtaata
    15061 ttaccaactg attttggcca gcatttttcc aatacaaaag attttacaac atttcctgaa
    15121 taatctatca tatgaacttc ctgatcttgc atatattgtg tagagttcgc tctatcgtgt
    15181 tctcttttta tacttctccc actttttaat tctgacaacg aattttgaat tatattttgc
    15241 cagctataca ttctctctat aatgtctcca ttaatgtcaa cattcatatc aactgaccaa
    15301 tcaccgtact ttctatagcc cgcaatttta taatcaagac tttgccaggg aattggaatt
    15361 tcatcaaaca ttgattctgg aattgttgtt gatcttataa gataaggcca cttatcaaaa
    15421 tcagatccta acccaaatgt tgtaagtgca ctatctgctg caggaacaaa ccattttttc
    15481 catccatcat ttgttgataa tactcctgtt ttccccattt ctctactaat tccagctggc
    15541 aattttaaga gaatatagaa taaaaattgt cttgatccac cggtaaatct tgtgcgatat
    15601 gtatcaacat caaataaaat ccgtggcatc tttcctccaa ataaaaaggt ctgaagagtt
    15661 tcagtaccct tcagaccttt cgatccatta gttttatcac taagcgtggt acggtatata
    15721 agtatttatt aatacctatt atccgaaagt tactccagtg ccataagtca cattatcagc
    15781 aacatgataa atgtaagtaa atgtcatatc aaactggaca acatcattag cagagtagtc
    15841 caatgtggcc gttccgaccg attttggcca tgcgccaaaa agtttatact tcataattgg
    15901 cttaccatcg taacctaaaa gttcgagctg ctggtcagcc atataaagat tcggtgcggt
    15961 gtaaatattt gttgttgggt cgtgaacaag ctgcgcccat tgcataaaca tttgctgaat
    16021 tttcgcatcg atatccacat taaaagtaac tgtccaatca ccgtatgtgt actttccggc
    16081 aaatttaaaa tcgaatcctt gccaatttgt caggatttcg tccgatgttg tttcaggcag
    16141 gtttgttgac cttacaaggt aagttgcctt ttcagtatct ccccctgcaa tactaatagg
    16201 aaatatcggt ttataataaa ataagtattg tcttgctcca ccctgaaatg atgccctata
    16261 actatcaata tcaaatcttg gcatttttta ttcctccaat ttattctctt atccagctgt
    16321 cgctccagca agctctgtga aggaggcgcc ggtcttggtt gcgataaagt tcagaacaat
    16381 aaattctgct gcccttgtgg gcttaatgta aatgtcacac cagagctcat ttctatcaat
    16441 tctttccggt gtattatttg tctcatcaca gacaatcata taatcataaa tacctcttct
    16501 tgaacgaaca tctctcaaga atgggtcaat catattgacc aaaagaagtc ttgtaagatc
    16561 atcatttggc tcaaaaagaa agtatcttgc tgctgtagca attgcctttt caagaacaat
    16621 gaagagtctt cttacattaa ttcgattgaa agcagattcc ttgtcaagaa gtgttttctg
    16681 accccaaaca actttgccct gaccagagaa cgagacaagt gggttgatac cggctttata
    16741 gagaatatct ctttctccct gtgttgggtt ccaagcaagt ctccttacat taccaagaag
    16801 ggctctgtta agaccagccg gggcaaacca aggatcagaa acatcatccg tattggcata
    16861 gataccagcg atatgtcctg aacatggaat ccatctatat ttaccgttcc atttgtcata
    16921 tacttcaatc cagttaccat aaatggaagc ataactggta ttttcattaa gtgtctgctg
    16981 tctataagtt ctaagtgatg ttgcctcatt accggagtta ttaataacat cagcataaag
    17041 acaatcaaga acagcaatac aatcctttct tgattctgca atcgaaacca gatattgttt
    17101 tactgtagtg gatttatttc catcaataag aatattaata tcaatttctt ctgcatttgc
    17161 ataaagatca atatcatcaa tgataagtgc atccgttaca gaatcatcct gatcatctgc
    17221 ccctcctccg aaactctgcc aagatgatgt agaaatcgtg atattttgat ccttttgtga
    17281 ttcattcata ctaatcctaa tataacttga tgattcatta ataactgttt ctgcgaaaag
    17341 tttatttcca ctatcatcaa caacatcttg atcagtagaa acattccaaa cttcctttgt
    17401 tgcataggat gtttctccct gtggttttac ctgtacaata ataaggaagg atttagaatt
    17461 aagaagcgga ctatcaattg ctgaaacatc tgaatatact tcccattctg aatgtcctcc
    17521 tgatgcaatt tcattatagg ttgtataatc aataacggct accctgatat tatttcccca
    17581 ctcacctctt gagttggcga taagataaaa tggccaaggt gcggttggtg aaacatcatc
    17641 cgcaaactga tctgggtctt tacttgggag gtctgataaa gtcagagcat cactaccaag
    17701 aacaaactgg gtgaatgttg cttctgaacc acttacagct ttcgttccag caaaagttgc
    17761 ggatacgggc attgttcttg tgcaataaag tgcatttcca tacctcaaat atcccgtagc
    17821 agcaagaata tcctgatagc aattcgccac atttgttggt tttccaaata tatctatcaa
    17881 ttcatttacg gttgttatca gagttttctt tctctccggc cccttgtaag tattccttag
    17941 aataattgct gcaatagaag tagctaccgc tggaatagta gtagacaaat ctatttcgtt
    18001 tacatctacg agtggtgaca aataaaatgc cattgtatga atcctccaaa tgttttcctt
    18061 atatatttat ggatatttca attatattta taaaaattta cccgatttcg tatctatcat
    18121 atataaatgt tgcgctggct tccagaacag cccccccctc tcttgttgag agagtaatct
    18181 ctccaagttg ttgaatccag acatttttaa agttaattgc cattattttt ctactaaaat
    18241 tatcggttat ttggagagaa gcatcaacaa ccatattatt tggtaagaca ccaaacttat
    18301 ccttgttatt atttatataa aatatccagt ttgcaagcgc tttccagttt ctaaattccg
    18361 aatctacaat gaaatttact gtccaagaat caaaaaccag tttcccaacc tgtaattgaa
    18421 gtttaccacc ctgccaagga gactctgctt gatctaaaga caagccagga ataactgtgc
    18481 caaacaggtt caactcaagt tctgcagtag ccgcacggga tgtttccgat ggaagagttg
    18541 gaaactttaa tctaaaatta gagggggttg ccctatgtat gtttactgtt attgccatta
    18601 tgtccttccc ttaccgccct ccggaaatac tgtatatctc gacaaaatgc ccgctgtttc
    18661 atcatatccg agggcctcga catataccgc ttcatcatac ataccacttg caccagatgt
    18721 aaatgttgtt tctgtttcct ctgccatggc ttcttctgaa gaataaattc ttgatataac
    18781 cttctcaata agtttcccat caggatcggt tgttggagta ataggctgca gaaaatatcc
    18841 ctgaatagtg aaagtcaagg tccattttaa tactctccaa tcctcttccc cccattcttc
    18901 cataatatcc ggtgtcgcac tattgaaaac tgtttttaat tcaaggtctg cgttcatttc
    18961 tggaacattg attttcaaga aaacatatgg attgaaaaac ggtaatatct gttcaagaat
    19021 ttggtcaata tcaacaatat gaagtgccca gatatttaca ttgagaagaa aactatatgg
    19081 tatcaggtta ggtattgttt caattgtttt tgcatcataa tcttttgata taacaatatt
    19141 ctcgttctta tttcccaatc ttgaaccatc atagtcaatt gacataaggg ttacggaaat
    19201 gatgggaagc ttttcttcag ttgaatactc ttttatccag taatatgcct tttcctttgg
    19261 cccgaatttc acaggaacaa gaacggtttt tgttatgttt ccgtcattgt cataccttgc
    19321 cacatatatg tctgctaaca tatcgagaaa ctgtataatt gttttcctga aaattctata
    19381 aaaataataa gttcgtgcca tttattttct tacgctgggc cgggattttc ttcctgtttt
    19441 ttcttgataa tttcctcaac atcacttata gccgtgttta ttgacaatat cttgccatgt
    19501 atccttactg ctttatagcc ttgcattttc cagcttttga gtttaatcaa tatttgcatt
    19561 ttagtcatat tattccacat tgaatttgga atttccttta ctttttcttc aaagagatac
    19621 ttttccaaaa gtttatctac tttattcatt gctacttttt cttctctttc ttttccggtc
    19681 atttcctctt tatcggttct tctccaatgt aaagtttgct cttgaaaatt caaggcggtc
    19741 aaccaatttc aatatattac ctgatctatc aataacacaa aaaccttctg gttttgctac
    19801 attgtaacct tccccactac ggataaatgt tcctatattg ctgatttcac tgaactttct
    19861 gacaaatata tctttcaacc tgatgaaaat caaataaaga tatagtaatc ccattatttc
    19921 tttcttatat ttagttagaa aatgatgcgc agaatctatc atttcccttc ttttctgttt
    19981 tgcttctggt gatttcacct gttctgcttg tttctcatat ttgtccttta tgaatatcat
    20041 aaaatcatca tagtaaagtg atacggagcg gagagcgttt gccttcttga tgttttcgtt
    20101 gtggaatatc atgaataatt gatatagccc gaagcttttc atctttttga aaatatcttc
    20161 cttgatatat ttcgcaaccc gctctgcttc tgaaatcagt tcatttatcc tgtcaatttc
    20221 cggcttggag aagtttactg tgccggattc atccctgaaa taggcatcct gaagccagac
    20281 atttggatta ttaaaatacc ttgatacatc tatattaaat ttggccgaaa gcgtatcaag
    20341 tgtatctcct gtgtattccg tgtgaacgac aatacccatt ttggctttat tgatcctgtt
    20401 tgcaaggtct gaaccgtcat ttggtatagc ataggttatt gtattcggtg taaaagtaat
    20461 atatggcatt ccgttgatat tctctatttt cttatcatct gtaaaaagaa gatctccctg
    20521 ataaattgtt ttcactccca attctgatag atacttcagg gaagttttca gcttagctgc
    20581 cagaccaggc gcatgaccga aatattcatt tacatcttta taattatatg caagtttcgg
    20641 tgtcttgttg aaaatggctt tcgtagaaac aaagaatttt ccattttctg gattaatacc
    20701 aaaaaacact gctgggctat tatgaataag aatatataca tcaccggctt ttacataaaa
    20761 attctgatta tgtgttgtca tatcacattc tggatatttc tgttttatct tattcacttt
    20821 tttgactttc atattctata agccctttca gtttttcaga ttggaaaaat gttttttcat
    20881 catcatattt acaaaaagag atggttatct tcccatttat tcggtttctt ttatattatc
    20941 attttcattc aattctatag ccttcaccca acccctattg attgtataaa attcgtgatc
    21001 ctctgtaact attattttat caccattttc caactcaact tctacccaat ttttattatt
    21061 atgatttatt cttggtttta ttgctaaatt caactcatca atacccttat caaaattata
    21121 agtaaaaact tccacatcat ttttaccaag aatctctttc attttcatat caccgttttt
    21181 cgtcttgatt attgtatctg gatgaataca accatcccat ttcactgtga tagccgcctt
    21241 tgatttcgat ccaccggaaa gtgtatcacg aatgagtttg gctaattcga tattcttttc
    21301 tataccattc acgccgttgt taaacatatt atcctcaaaa tgttctatat gccgatttat
    21361 atcttcattg agcacttctt gaatggtata tattctttgt tccttgaaga tttggcctct
    21421 gacatttcca ggccttccaa tggttgccct gagaaattcc ctgaatttct caaaccactc
    21481 cttattatcc aatcccctcg ctacatttct tttatactcc tcgaagtcat tttctccgat
    21541 agcctttctt accttagatg cagaaatatc ttctgctgtt ctcttgattt catcaaagat
    21601 tacattgtaa tccactccga taaactgttt tttatagtct tcaatcctgt cagaaccggc
    21661 tatgattgca acaccattgc ttgtttcaat gttttcctgt ttaataatat ctggaatata
    21721 accgtgagaa gataaaatcg gatgtgcttt cgggttcgtt atcttgagaa tggattttct
    21781 ctgattgtat gtaaggaaat ttttggcgag ttttgaggat ttctctccct cgatgataaa
    21841 aatgtatacc tttgaatatt tcttgagaga gtcttcaata attttttggt gggcttttgt
    21901 gggtggtgac attctaccta taaaaatacc caccgctcgt gacttctgtt ctgtaatgta
    21961 atttgaaaat ctcatcttaa taatgatatt tactaccagt atatatgtat ttctttccaa
    22021 accggtgtta catttctata cttatctctt cattccctcc agatctctca tcagagtgct
    22081 cctttcgtgt ttctcccaca tatcaatttc ataattcatt tgtttgatta tttcatccgg
    22141 aataccaagt ttttttcccg ttttagtgtt tacatctttt tttattttca ccatcctgtc
    22201 aaagtttttg agggcttgat ctggctcatc cctatatgtg tcaaagatct ttttgcgaat
    22261 aacctcaaga tctttgatag atgtctttac aagatatttt cccttttcca accaatccct
    22321 gtctttgtta aattctttga aaatttcttc tatcttattc atatgctttt cagttccccc
    22381 gtttttgcca agtatagagt tattgcccat atatcagctc ttatgttctc tggtacttga
    22441 cgaacatcac ctttatattt taattctctc tcaacatact tctgtatttt ctgttcctca
    22501 tctggacttt tatccaatat ttttatatat tcattccata gtttattatc tatatctccg
    22561 agcattgatg aaagccttct tgcatcatca atactttttg ctgttgcaat tttgaacata
    22621 aattttgtca gatcccgcat accaattgct tctgtcaatg ccattaaatc actttttctg
    22681 tagagatatt tgtcaataag attgtctatc tttttcatat gtttgttctc tgtattatcc
    22741 tttcatctgg atttaatccc caattggggt tttcttttgc ttttttaagc agtgcttccg
    22801 gtggttcacc ttcaatcttt tgaggatgaa agagtattct gtattgaggt cttcttcttt
    22861 catcaaaata tgcaacaaga acagaccagt tattatattc ccttatcaca actcctttca
    22921 acataacttt cttgtttatc tgtgctctgc ctgtaacata atctccactc tgaaatttca
    22981 tttatagctt gcccccacca tattttttga atgcctcttt tttctttttg tctatcacag
    23041 cctgttttat tttgggattt ctttccagct tatcagcaaa ccacctaagc gtatcaaata
    23101 ttggatcttt atcgtcatat tcacccactt tccttatcca aaaccaaaaa tcatcttctt
    23161 catcggcgag aaccagcttt gcgttgaagg acaattgttc caagtttttc tcaaaatcat
    23221 tctctatttt tattatctca ttatctgcct ttaccagaag atttctgatt tcatcccatt
    23281 ctggttgctt tttgtatgtc tcttcgctca aatatctctc aatcttctcg tctattcttt
    23341 tcatttacta taccatccat attttttggt aatgaatttt gcgtgttctt ccatttcact
    23401 tacaagctca tctatcaaat ctttggacat atatggcatc catatttctt gggcaaagcc
    23461 gataagacga gaataaaagc gccaccaaca atcagattgt ttttctatat cacctttata
    23521 taaatcgaca caagattctt tggcatattc atccatcaca ctcaatgttg cagatacgct
    23581 ttccttgaat gcatcatccg ctttcttctg tgcgagtttg aaccggcttt taagtctccc
    23641 gtcctattca gaaatatttt ctttatcatt caaatatttt ccaaatttca tttcttcctt
    23701 cagaccatat tttttgagaa tggctctcgc ctgttctttg gtcataccac ccattatcct
    23761 tgccattaca tcattcattt tcaatgtttt tctggccatg gctatttgat gttttacaaa
    23821 tggatctgtt ggcatcttct tgatcaaata cttttcaatt ttctcatcta tttttttcat
    23881 cttacaccat acctgcgtaa tttttccaga tcatttctta tctaccggat ggaaaaatat
    23941 attctttcct tttataacta taactgcctg acgggccgta ccgctttatt ggaatttgaa
    24001 atggattttc tacactatat tttttaatca ttgaaagtct tttattttct ttgaccattt
    24061 ttgactgatt ttgactttca acaccatatc ttttaataca ggtctttttt gacttttccc
    24121 tttttgtttt tttttcccta tcctatattt tcctatttta ttacaaccgt attcacatat
    24181 gttcataatt gatatccctt ctatatgttt ttgtatctat attacaaaat tatttatata
    24241 gttcattaac atttttaaca aaggatatcc catcaatgta agatatgcaa taacataacc
    24301 gtagatgcga gtgtcgatat catcaacaac atttgcgtgt tcttcaatcc aagtattatc
    24361 accaaacgcc gaaataagtt tgctggtaac ggcaatatcc gcagcacttt cgctctgctc
    24421 tgaatatctg aacggcctta atatcaatac ccatatcatt ttcttgagct ggaaggtttt
    24481 gtcatcatca tcaacatgac agacctcaaa tgatctgtta gccaattgat accatttaat
    24541 atgaacggcg tcgcctattt tcggacttac tgttttacta acatctctcc tccaagtact
    24601 catcgggata tgtgcagtaa taacatcacc acctgtcatt ccaaaagatg accacaaatt
    24661 aggttcatcc acgacttcgt aaattatttt tgtctccttc ggtgcctcat atgtagttcc
    24721 tgtatgttca ccatatagcg tatcatactc tacatcattt gacctgatat aataatttat
    24781 cttaacaccg gcaatatcgg tatactccat aatcatgctt tcggcaagca tatgctcctg
    24841 attaccttct atatcataca aattccattg tggtttctgt aagccagttc tattctgttt
    24901 gttcattttt gaattatctt catttgtaat tttgtgaatg ctcccccccc ccctctaaag
    24961 ggagtgggct tctaaagtaa ccttacgatt acttaaagag tccattccga ctcttaaaat
    25021 gttaaataaa aatttataaa aaagttatcc aatataaata taatagccat cagcctataa
    25081 aaattcccaa tccctcccaa ctttcctcgt tcctcaattg ctcttccagt ttttctattt
    25141 ctgtttgagc ctctgaaagc aatgtatcac catcaagtgc gaggccagtc tgccctatag
    25201 ccgtaaaatt agcaaacttc gatctaattc ttcccagtgt tttcttactc aaggctgtgg
    25261 cataatcaaa tatccacata ttatcatata aatcctcttc cgttccttcc aagacatatg
    25321 ccctaacaag aatatagcct ggagaatcca accaagtgtc ctgccaacga attgttcctc
    25381 ctgatggtgg tggcggttgt atttcaagaa tattggtata tctgtggtaa gtaaagttgt
    25441 atgtgtcaaa tacgtatcgt tttattgtgt ccaaaaaatc tcttgctatg tgataagata
    25501 ccaatgtata tcctgacccg ccacctctca tcagcaacat atcatacata cctgcattat
    25561 acaggtaatt ttcaattgta aagagtgtat ggattgatcc ggtggttcgt gtgtcataac
    25621 tcaaaacttc tgtaacatct cccggcaact cataatcagt aacaccggct gatagtgcga
    25681 ttgtaaaata tttttctact acaccttggc caactgccca tttgataaat ttctgtctag
    25741 cataatcaat atgatcagct atttgcatat catccagttc aactttgaca acaggataac
    25801 caagaatcct cttaatcctt tctatcaact ctgatttttt tattgctcta tatggtttaa
    25861 gcattttatt ccccagcctc tattttatct tcaatgtcct tcttcattgc tgtcaacatt
    25921 tcaatagcat tgtcaatgaa ggtaaatacc attttgtcat tataattcag atatgatcgc
    25981 tcaagggatt tcaatatatc cattactcta tgaatattaa cgggaacttt tgcaagaggg
    26041 tgtctactcc caatatcgtc gttcaatagt ttgctcatct ttctatccat tcactatcca
    26101 cggcttccca aaaggcatca acaataggat gtgatgttcg ttcttttggt ttcataacat
    26161 attctctaac cagtttttga tacatatcct tgaaaaaatt atcaactgaa ttttgtgata
    26221 tgccccacgc accctcggcc tccgcgtttg ctttatctat ttcttctata acatcttcta
    26281 ttgcctttct aaattgaaaa accatctttt tcttcgcttc taatagtatt tctttggccc
    26341 tttctacttc tttatcaata atgttttcaa ttacattata ggtcaacctt atttcggctc
    26401 ttgcccggct ttctggtgtg atgacttctc ctaaatattc ctcaattttc ttgtctatag
    26461 acatattctc tattggcaaa catcattccc ctattaaata ccaccgccat tttcatttct
    26521 attgtttctt cgtttaaaaa gttattgatt ttcctctcaa tagacattga atccccccct
    26581 gaaaagttat tataattctc tattatttat aattttaatt aattaattta ccagttcttg
    26641 aaagccagct ccaatcctct gcaatcggtt caatatcact taaaacaccc caaacatcgt
    26701 cctctatttc tgtctttttg atctcaaatg attcttccag catatcggtt gtggtaaaat
    26761 aacatcccca gtataaggct gtaataaggt catctggcat atctttccca aaaaatttac
    26821 cttctttttc aataaatgtg gatatttggt ccacagtatc ataatcaact attgtcaaac
    26881 taccatcttc cacaagcttt ttcataagca tgacggctct tggcttggtt ctgcgatctg
    26941 ctcttactcc taaatccttt gtttttgagc cagaattaac aagaccgggg ttttcatact
    27001 cccaccatag ccttgttaca acagttgagc cctctgcatt gttctccgcc ataataaagg
    27061 ctgaattata atagattgat gtcctattta caatgtccgc aaatgtataa acatcaatat
    27121 aattatcatt gaatgtcgct acttgttgca atataattgg tttgacctga acaattttca
    27181 atacctgcat agtagaataa ttttctcctg ttccctttgc tgtgtcaaca ccaataacat
    27241 aaaccgcctt ttctactggt ttttcgtata ttttgagttt cccattggcc tgttcaacta
    27301 tcggtctggt gcatttgtca tataattttt caataatgtc ggaatctata actgtatttg
    27361 tagaaccaag aaattcacat tcatattcct gtataaattt ctgtttacca agattctttc
    27421 tctggtccga agcccatttc ctgtctctgc ccggaacatc cttccaagtt gatttgatag
    27481 caacgaaagt gttttccttt ctttctgctt gagtataaag cctgtgatat agattgaaca
    27541 taccatttgg tgtggaaatg ataacaactc tggactctga tgaggcggca attgtaggaa
    27601 ggttggatga ccagaactct tccgccttgt ttttctgaac gaaagcaaac tcgtcaagta
    27661 tcaggagatt tatagttcta ccacggaaag aatcgggaga tgtagctgat accataattt
    27721 ttgagccatt atcaaaattt acgaatgttt tggaatattc ctgaacgccc ggtttcaacc
    27781 agacgggaag ctcctcatac attattttca ggcgggaaag aatgtcaata gccgatatct
    27841 gtttattaga cgcaattcca acgattttat cgggattaaa tattgcatac cagagagcgt
    27901 atgcacatac ggtagtcgta ttatgtgttg gtatcatcgt ttttccacaa aggaaaagat
    27961 gttcatcatt gtctacctgt atacatttta caggaacaga attaaccttt atgatatttt
    28021 tgatgtatat tcttgtattt ttatcatgtc caaaacattt tgtttgtcta tctttttttc
    28081 tggaaagatt gaaaacagag aaccgtgttg tggaaaaaac aagtgaatga tatctttctc
    28141 catttataac tctgtaattc aattttgttt ttattccaat agatgaaaaa atttctctta
    28201 tatcttctat aagagttgaa gatttttgag agaactcaca aaccccatct ttcccacaat
    28261 aaccatttgt gtccatgaga cctcgtataa gttcaagttt ctgatttata gaagctgaaa
    28321 aatatacttt tggtatatgt ttattattga tgagattaat tttcctcaaa tcaatcatta
    28381 atttataaac attaaaatac cccccattct catttctcca atctttttta aaagatgatg
    28441 ttaaatactt ttcctgtatt attttggaaa tctcattata gtcatttata gatgtagata
    28501 ttcttccatt tactaaatga ccttccccca accatatacc aagaatatat gggtctacgg
    28561 gcaattcaat atcgtcattc accacaccag agtgataatt gatataataa gaatgagatg
    28621 tattactgaa tttatcaaaa atttcttttg ttgtaagaac tctttcttcg tgagttgttc
    28681 ttggattgtc ttgactattt ttatgaccaa aatttgatga acccacacac cataaatgtt
    28741 cctcatcaac aactattctt tctccattgt caaattcaat ctcataacaa ttatgatttt
    28801 ccataacttc tgttacaaaa acaacttttg tgggcttacc atccctccca atgagaaaat
    28861 cccccacttg aacatcacct atcgttgtcc atttaccatt gggttgaggc aagggagtat
    28921 caagagcaag tgcttttcca gactgcctgc tccaaagacc aatgacaaat ctttcatctt
    28981 gtaaaatttt tagaattttt ctctgataag gatacggctc aaatgctatt cttcccctat
    29041 caggatgaat aattttaaca tatttcagga actcccatat atcttgagag catttagaca
    29101 atgcggctac ttcttctggt gaatatgaat attcctgaag cggccttttt ataaaatcat
    29161 catattttac tggcacgtaa gaaaaccccc ataaaacata ctttcatatg tatttatggg
    29221 ggcaaaacaa taaaaagggg aaactataat gaaattggag ctaagtccat catatcaaag
    29281 tgaaaagttg aaaatggatt gtcagacgcc tccatatttt ctgaagaagc cgggggggag
    29341 actgttccat cctctgtata atacagctct ttatctttac ctgttttctt atcccttgta
    29401 aaagtcttta gtgttactgg ccttccccat aggtagtaaa tatgcttcaa tgtctcttgt
    29461 gcatattttt tgtgaagagg aataccgtta tacctatgta caagctctaa atgcccgccc
    29521 ttgtcaagat ttccattgac aatctcaatc ttgggtattc cactgtttgc aaagctattg
    29581 ataattaatt cccttacttg ctctactgta tgattggttc ttacataatc aatagaggca
    29641 accgtttcca ttatctgata aatataaaga tcgagtttat caatcaaatc aaccgttaga
    29701 aaatcttgca taaagaacca atcagtatag ctcctcataa cctcaaacat tttctcccgg
    29761 cctttcatct cctttgtatc ccaattttcc ttagttttcg catctgtgca gttatcataa
    29821 tcggtaccgt atctaccctt gttccacctc tcttcgattt ctttccacat ctccgagccc
    29881 accagatatg ggttcatacc catcttattt gtggctttga caagagaatt actataatta
    29941 tactggccgt gttcttctgc agtgagtaga ccttcatcaa ataatctgtt cataaagata
    30001 ttgtgccagt aggtcgccca gccctcattc atatatttgg ttttggagta aggccaaaaa
    30061 tactgtcctt ccattctcaa aatttccaga atgtccttct gccaatcttc cagaatacgg
    30121 gaattgtcaa taatgaaccg aagaatatct tctgtgggtt ctatcggggt tgtagcaagt
    30181 atctttctcc acagcctgtt attataaaca tcgtaatcct ctgccacatc cttcttggta
    30241 tcacgaacaa tatcaccata aacagctcgg gaaggaagtt tctttttctt catttgctca
    30301 aagactcttt tcttcctctc catctccgtt tcctgattaa atggtgatga gtgccattgt
    30361 agggcgtgtc cagcatcttc agttttctcc acctcatcaa gaccatatat tctttcatag
    30421 tcattgaatc tcttctgggc agcagccagc acttcaacaa tatcctcacg gaaatttaca
    30481 taccatttac tttccgtaaa gaaattgaca tgcgcataaa cgtgcgccat aacaagaacc
    30541 tgaacagcca atgtattgga attcatcaaa aaggctcttg aaggattatc gtggacaacc
    30601 agctcaagag gaaggttcgg atctactgtg tcaaatacag ttttcaatct ttcataatcc
    30661 ctaccatatt tccaattgga gatatttgta ggcatcctgt aagccagtat ctctatcatc
    30721 ttttgtggtg gaacgatatc aaactctaca ggcagacact tcattccata ttcttcaaca
    30781 tactgtttaa ttctatcttc aatttttatc aatctctgca aatcattttg tttcattatt
    30841 ttttattcct cttcttctca aataacatat gtttgagtgc aggatatata tgttctttcc
    30901 cttttatgat acaggcaaga aaatgccttt cattatcctt atagaaattg gttccggctt
    30961 ccgtttcaat cttgaagtca aatgtattgg tgtatttttc catcaaattt ccgtgtgatg
    31021 cgtggaaata tccctgtgag gaaatatgaa tttcacaata tcccagcata ttaatcttct
    31081 tgtccagaag aagttttgtt gattgcatgg tttcatcagg attaaaatcc tctccatcag
    31141 aaacatatac aacataaaca ttccactggt ttacaggata ttccctttca accagatctc
    31201 tggcaagatc aaaagctgta tgacaatagg ttcccccact ctcgcctttc ttgaagaagg
    31261 tttcctcatc cacaagagag gcgatagtgt gatgggcgat aaaccgtatc tgaacatggt
    31321 tataggtttt cttcaaaaat tcaaccatcc agaataacat tgatctggca agatatttct
    31381 tttccggtgt cattgacccc gatgtgtcca tcatggcaat aacaacagca ttactgtgaa
    31441 tttcaaattc ctgttccatc tgtttgaacc tcaaatcatc atcctcgata aaaagagatt
    31501 gaacatcagc atcaggattt accttatttt cttttataag attgatggca tcaaccaaat
    31561 ctccgtgaga ggttgcaagg gccttgtatg ccgtttcctc atcacacttg gtttcatcca
    31621 taatttcagc agcataaata gccatccttt ttatggattc tttgatggtt cttctcttgt
    31681 ggattcttgg atggatgcct acttttgaaa cggtttcgaa tttccatccg gaaggaacaa
    31741 gtaattctgc ttttgttttc tcttcaatat atggaagacc caaatcttga aacataatat
    31801 tgataagata atcaatatca acttctgttt ccatataatc gtcaccacgg gcattaccgg
    31861 gcttactgtc atcattcttt tttcctttac ttcttcggcc tataatatct ccagccttac
    31921 ctttaccctg accaacaccg cctgcagttc ctgtgcttcc ataaatgaac cgataatccc
    31981 tcaatccctt tacaggaata cgcacagttt taccgtgctt ttgagtgatt atagattcct
    32041 ctgcaataat atcttttata ttttttctaa ttgtatcttc gattttctca cggtgcctgg
    32101 cagcatcttt tctacccctc gaagataaat cccaattttc atgcgaaatg atcatattat
    32161 tcccctttta ttgtgtggtg gtggcggatt tctctgccac caccacctcc cgaagcatct
    32221 cacaccgatt tggaggagat tagttatttt tcattttcat aatatcttca tatatcatac
    32281 ttcattctac ttctcctatt cccccactac ttttctatat gtgtataaaa ttttgtgagg
    32341 aatttatatg aattatctac ttgatgttat ttcactcttc cttcctgagt atttctccaa
    32401 cgaagcttaa caatatattt gcacactctg ggcagtatcc cctcttagta agatttgtga
    32461 cggctgattt tctcttcatc tgcgtcctct tgtccgtagc agacttgtct gcgagcgtaa
    32521 gagataccac atttttgaga tcactcatca acttcttctc aatggcgata cggagaggat
    32581 cataggtttt gaaagtgaag gcttcaccct tctccaattg ggcagattta taaacgaata
    32641 taccgtttct aaattctgtt ttagaattta ttggaatacc aatatactct tcaatctgtc
    32701 tcatcagctt ctcatcggga tcactatatt cacctgtaat cgaatcttga attttctcct
    32761 tacgacagaa agcatcggca tttcgaatat agttttcgaa gagagattgt gcctgctctt
    32821 cataagcgga gagaaacgcc atattgactt cctttttcgc tatatctttg aactcggcac
    32881 ttacagattc cttttcaccg agaagaagtg taagatattt ctttgattct tcttcggtga
    32941 ttccgatttg gtgctcaaag ttagaacgaa gagctcgaat aatgtcaatt ggattgacac
    33001 acttcttctc ttctttctga ccaagtgcaa tgtttagagc gttgatgatg aatcttgggg
    33061 aaattcctct cattccctca ccctgctcct taccttccag acggagagct ttgacatcaa
    33121 tctcctgttt ctt
//
LOCUS       DAOTQL010000004        27186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_1849_length_27186_cov_5.518005, whole genome shotgun sequence.
ACCESSION   DAOTQL010000004 DAOTQL010000000
VERSION     DAOTQL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27186
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_27186_cov_5.518005"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="P5293_00455"
     CDS             complement(<1..240)
                     /locus_tag="P5293_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRR48437.1"
                     /translation="MDNGTEAQRYAQRGVSSSKEEVHNAIKDDDKGLFPDAFCKIVAD
                     KLSNDPEYCLVMHADGAGTKSSLAYAYWRETGDMSV"
     gene            complement(243..2429)
                     /locus_tag="P5293_00460"
     CDS             complement(243..2429)
                     /locus_tag="P5293_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRR48438.1"
                     /translation="MSSFRKRALDEFASHKAERFYVENRPVSEFFAEKVFDVEKMRRY
                     LSAEAFEAVQQAISEGTKIDRSIANQIASGMKSWAVENGATHYTHWFHPLTDATAEKH
                     EAFVDPSKNGGVFENFRGDALVQQEPDASSFPHGGLRNTFEARGYTAWDPTSPAFIMD
                     QTLCIPTVFVSYTGEALDHKAPHLKSLQALDRAATDLCKLFDHRTTRVNVMLGIEQEY
                     FLVDEALYRARPDLMMCNRTLLGHTSAKDQQLEDHYFGAIPSRVMAFMKDFETEAYKL
                     GVVLKTRHNEVAPNQFEFAPYFTEANLAVDHNLMMMILMRKIAKRHKLKVIFHEKPFS
                     GINGSGKHCNWSLITNNGVNLLAPGKTPRTNLQFLSFFVSVIRAVYEHHPLLMAGVAS
                     LNNSYRLGGDEAPPAVMSIFIGKLLSSVIDSIEKMSNGEESIEDLHHKLDIVNKIPEI
                     LPDNTDRNRTSPFAFTGNRFEFRAVGSSVNVAAALYPLNAAVAQQIRRFKFLVEVYMK
                     DGIEKDEALLKVIRQFIIESRNILFEGNGYSPEWHQEAERRGLRGIKDLPNAFNTYLE
                     ENSVRLFEETGVLTRREVEARYEVMNETFVKKLQIEARVLGDLTQNHIIPTAIKFQNT
                     LIENLRGIKELFDEEEYRQMSENQLMTIRKISGYIETLRKNTKELIEDRKIANKVEDM
                     AQRARLYYDNVLPTMTKIRIVVDKLEMLIDDELWPLPKYRELLFWR"
     gene            2539..4020
                     /gene="dacB"
                     /locus_tag="P5293_00465"
     CDS             2539..4020
                     /gene="dacB"
                     /locus_tag="P5293_00465"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRR48439.1"
                     /translation="MKKPSLFLLIFLILATSFNLSAKNRIVQKCVNQMKKDTLFSNSV
                     VGIMVMDSKGKCVASWNPDSPLLTASTMKTISTGLALQLLGPDYRFKTRIGYSGQIEN
                     GVLNGDLYIVGGGDPTLGSKDTVAIPVDTVFSQWYKFIRAAGIVKINGSIVGDDRFFE
                     EEALPDTWSWSNIGASYGSAASGLSFCENIQYFTFIPGEKTGEPVVMKDVYPSIPGME
                     YRNMLKTGISGSGDRSSYYVSDLAKVGVFKGTLGAGKESYTVDCSSKFPALMCAEEFR
                     LFLIDKGIESNPDVKDVRSFDATDQDQLNIIGETLSPRLISIVNVTNRISNNFYAETF
                     FKMIGKQLTGNGSYDSSVVAAKRELAKMGLSLKGYTQVDGSGLSRQNYVSPRFFCKYF
                     ARMKEMNNFAEFIGSFPQPGGPGTLKSVLKDADSVTKSKIHAKSGSLSNVKCYAGYVE
                     KRDGEYDYFAILTNNYQARTSEIQAGVERFLGALVDIKETSKK"
     gene            4049..5251
                     /locus_tag="P5293_00470"
     CDS             4049..5251
                     /locus_tag="P5293_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRR48440.1"
                     /translation="MLTLSKKSEIMPASPIRKLVPYANEAKKRGIKVYHLNIGQPDIE
                     SPREALDAVKNNTLKLVEYPDSKGNESYRKGLVKYYESINIHVTENDINITTGGSEAL
                     QIALAVTCNPDDEVIVMEPFYTNYNSFALQNNVVLKPITTSIKDGFAIPDPSEFEKRI
                     TPKTKGIIICNPGNPTGTLYSKESILKIGEIAKKHELFIFSDEVYREFCYTDQPHFSA
                     MHIPGLEENVILIDSVSKRYSLCGVRIGAIVSKNKAVMNAVLRFAQARLCSPAYGQIA
                     AEGALATPKSYFEAVRAEYIKRRDFLIEALNKMEGVYSPMPMGAFYTIASLPIDDSDK
                     FAQWLLEDFQYEGQTVMVAPAAGFYATKGKGTNEVRIAYVLKIDDLKAAMKCLEIALK
                     QYPGRTTK"
     gene            complement(5244..6218)
                     /locus_tag="P5293_00475"
     CDS             complement(5244..6218)
                     /locus_tag="P5293_00475"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HRR48441.1"
                     /translation="MQYDKRYNFAVSLPYLILLLFFVVFTSLKLPDDNFERWIPLSAG
                     FIEPEDIPTLYSHLLAAFFLIISTISLYIFNERYLMTGKEGLTLPLTYLVLAFSSYHT
                     SLFSGGSIAVVLIIWSIYTSLKIRNNEQYSFLAVFLATIASLFEPLMIYIIPLVFFFS
                     IKETGISMRRLVLILLAFLIPVGIIFSLRFIFFNDAHIFAEEYLYHLKIGNIAKPDIR
                     SVSHLFFTIFLLLLLILSLRDIFNRINRFKIIQSASFVRLIILMFFLSGIIILYPDSS
                     GRIMQIFSFPSSVLIVESTGKNENSHKQRLYFFLVTIFLILGRIACFI"
     gene            complement(6229..7521)
                     /gene="purD"
                     /locus_tag="P5293_00480"
     CDS             complement(6229..7521)
                     /gene="purD"
                     /locus_tag="P5293_00480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRR48442.1"
                     /translation="MRVLIIGSGGRESALAWSISKSPLLEKLFCAPGNPGTALIATNI
                     DIDIKNFEAIKLIVMEHNITLVVVGPEEPLVNGLRDFFESHDQLKNCLFIGPGKKGAL
                     LEGSKEFAKNFMQKWSIPTASYMCFDSSSMHEADYFLEKLSAPYVLKADGLAAGKGVV
                     IHSNIDDARRELQAMFNGKFGTAGRKVVIEEFLKGIELSVFILTDGKEYLILPEAKDY
                     KRIGDGDTGLNTGGMGAVSPVPFAGEEFMRKVEERIIKPTIQGLVRDKIDYKGFIFIG
                     LMNCEGDPYVVEYNVRMGDPETEAVLPRIKSDFLSHLVAAAQGKLSGEKIVTDSRVAM
                     TIVAVSGGYPEDYKKGYLIKGLEKFEQDNDILLFHSGTKVSDGHVVTSGGRVLALTTL
                     APDIHKCRQILYKEIEKISFNDIYFRKDIGLDLMRYSK"
     gene            7585..8250
                     /locus_tag="P5293_00485"
     CDS             7585..8250
                     /locus_tag="P5293_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRR48443.1"
                     /translation="MKIIAMRRFYSIFQIIVIIITTTSCSKKDIIPRKELPQILAEMY
                     IADRALLSDPAKLRQADSLLVYEPILKKYGYTTKTLINTFNYYLPTPAKLKSSFMAAR
                     AILEKRLVEVQNQIALMQKREIVFATVHKLIREADSLRYMDSYERSLRWLLAPELFPN
                     RPVYIPDSLISRYEHPKMTNWWLNNLKTESRHFYQYENNSSTIPLLNRPQPNQERISL
                     PEH"
     gene            8171..9373
                     /locus_tag="P5293_00490"
     CDS             8171..9373
                     /locus_tag="P5293_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR48444.1"
                     /translation="MRTIAAQYLFSIDHSPIRRGYLCLNTDGVIVEVGQLEENEERES
                     TEFYDGIICPGFVNSHSHIELSHLKDHFSEGSGMAGFIRQINALRECSSVEERVAAIE
                     REMKQLFESGVSAMADISNCDESFRIKSVSQLYTRTFIELFGSEPEECETIMHNGFKL
                     QQIAKSYDLDAAITPHSCYTMSPELNKAAIKAALKEGFLSYHNQESWEEEELIKNGTG
                     PLAEEYKRRGLSTPPVIGKPALFYFLQNLSEVRNLSKETVKEHILLVHNTFTNEESID
                     AAMTMISNLFWAICPLSNIFIHRCLPPVELMRRKGCTITLGTDSLSSNKVLSIVEEIK
                     VIHNYFPNISLEEILKWATLNGAMFLGKEKELGSFSTGKKPGVVLIDNIDWNKMQLTE
                     NSHSKRIV"
     gene            9389..10165
                     /locus_tag="P5293_00495"
     CDS             9389..10165
                     /locus_tag="P5293_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR48445.1"
                     /translation="MSTILFNEIIYGPIKSRRLGSSLGVNLLPPNGKWCNFDCLYCEC
                     GFNKDGKDNRQIPAREDVRKSLERVLSNLSTKGDRIDSITFSGNGEPTMHPDFAAIIE
                     DTISLRTKYKPEAKVSVLSNGSGIARKEIVDALLKIENPILKIDSAFDRTILLINRPA
                     YAYSIRKLKHDLQPLSDRFVLQTMFLRGEFEGKTIDNTTPEEVEAWRALVDEIKPREI
                     MIYTVDRETPAKNLCKVSVEEMNKFVAPLIEKGYKVKVSG"
     gene            10167..10625
                     /gene="dtd"
                     /locus_tag="P5293_00500"
     CDS             10167..10625
                     /gene="dtd"
                     /locus_tag="P5293_00500"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRR48446.1"
                     /translation="MRIVVQRVLEASVKVDGICTGKIGKGLLLFAGFEDNDNDSDIAY
                     AAKKVVNLRIFDDDNGVMNHSILEKCGGILLISQFTLFAQTRRGNRPSYIMAAKPEKA
                     IPLYEQFRKELEKTLGKKVETGVFGADMKVSLINDGPVTIWIDTKVDKNS"
     gene            10629..11459
                     /gene="deoC"
                     /locus_tag="P5293_00505"
     CDS             10629..11459
                     /gene="deoC"
                     /locus_tag="P5293_00505"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRR48447.1"
                     /translation="MENIKREISKITDNIDNWLNKEALRKYIGLIDLTSLNSADTSSK
                     ILGLVEKVNNFNIDFPKYPSVAAMCVYPNFAKLLKSSLKVKNVKIAVVAGAFPSSQSF
                     KEIKTAECRMAVENGADEVDIVLALNKFLDKKYEETKSEIRDIKAVIGDKHLKVILET
                     GVLKEEHLIDYAAEIAIESGADFIKTSTGKIEPAATPEAAYIMCKRIKAYYEKTGKRI
                     GFKPAGGISTPKEAALYFAIVENVLGDEWLTPQLFRFGASRLANNLLTEVEGKVINYF
                     "
     gene            11470..11934
                     /locus_tag="P5293_00510"
     CDS             11470..11934
                     /locus_tag="P5293_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48448.1"
                     /translation="MKKTIVLLTALLITVSAVAQNYHRAVSARFGTSVGVSFKQFVSS
                     AAAFELMSSVDLVENNNQKLIFSAYGQYHYDKLGVDGLSIFGGPGTSAGLFISGKYQD
                     KAVLSFDIITGVEYKLQELPLILAFDWNPKLQMITDAGIKPANFGVTLRYTF"
     gene            11945..13300
                     /locus_tag="P5293_00515"
     CDS             11945..13300
                     /locus_tag="P5293_00515"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRR48449.1"
                     /translation="MIKNYIKENSDRFLNELFDLLRIPSVSSMAEHKQDMQKAAEKYV
                     HLLKEAGADKVGVYQTKGHPVVFGEKVIDKSLPTILVYAHYDVQPADPIDQWSTPPFE
                     PVIKDGAIYARGADDDKGQGFMHVKAFEYMVRTGQLKCNVKFIIDGEEEIGSPSLSEW
                     AAEHKEMLACNDILVSDTTMIDEKVPSLNVGMRGLTYMEVEVTGPNKDLHSGHYGGAI
                     RNPANALCSMIDKLIDDKGRVTIEGFYDDVVELTKEERDMLAKAPFDIEEYKRFLDID
                     DVAGEEGYTTMERTGIRPCLDVNGIWSGYTGEGAKTVIPSKAYAKISMRLVPNQDHNK
                     IAKLFENHFLKLAPKGVKVKVKHHHGGQGFLCPISSKVYQSASRAITEVYGIEPVPSR
                     GGGSIPVLAELQQILGANPLLMGFGLERDVIHSPNESYLLSQFYKGIESIALFYKYYT
                     E"
     gene            13406..14245
                     /locus_tag="P5293_00520"
     CDS             13406..14245
                     /locus_tag="P5293_00520"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRR48450.1"
                     /translation="MKIGIIGFGSMGRMLMHKFLETGAIMQEELYVSNRTTEKLSEIS
                     FFYPKVNICNNESLVKSSDLIFICVRPAQMKDVAEIITPHIQNRQHICAVNACIRFNQ
                     LDSIFPGTSCSIVIPSVTGEVNKSVTLASFNSYVSKHEVDLIKSLLSSFSSVLVIPEN
                     ELGICTDLTSCMPGFIASIFDIIVDEASQHSSLSKDEIHSMIITTILGTSKLCVDQKM
                     SFDKIVERVATKGGITEEGEKVIRSRFPEIVKSLFQHTREKRENTINNFSFEPKCEKC
                     KKL"
     gene            complement(14255..15361)
                     /locus_tag="P5293_00525"
     CDS             complement(14255..15361)
                     /locus_tag="P5293_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRR48451.1"
                     /translation="MYENDSRVVVTLDAGGSNFVFSAVQGNDYVMRPISLQTNASDLD
                     NCLAILVKGFEAVIRSLGETPVAISFAFPGPADYKNGIIGGYLPNFPSFRDGVALGPF
                     LEKKFGLPVFINNNADLFTYGEAIAGALPEVNKRLREVGSIKQYNNLIGYTWGTGFGF
                     GISFNGALHIGDNSCTETFCLRHKLMPDIIVEEGVSARAIKRVYGELTNQLVHDLEPL
                     DIFRIANGVREGDRNAAIKTFELFGEVAGDAIVTAISLIDGLVVIGGGITAASKFIMP
                     SLLREMRSSLKTLSGENVGRLQMSVYNLDSEEEFEQFARGQSRELKVYGCNDKVIYDP
                     EKRTGVMISKLGASKAISLGAYCYALHNLDSNNY"
     gene            15542..15781
                     /locus_tag="P5293_00530"
     CDS             15542..15781
                     /locus_tag="P5293_00530"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRR48452.1"
                     /translation="MKWVTILTAAYPQDILIPQSNLEARGIHTFLKDEYYNSIVNFYP
                     NSNVKLQVPEDEYDTAAEILKAAGYEIMPKPPESR"
     gene            complement(15753..16991)
                     /gene="clpX"
                     /locus_tag="P5293_00535"
     CDS             complement(15753..16991)
                     /gene="clpX"
                     /locus_tag="P5293_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRR48453.1"
                     /translation="MTSKFNDYCSFCGRDKDEVDVLIVGEIACICSDCAQQAVDYARE
                     VMKARKPKGSQEEDHQRRLMKPLEIKSFLDQYVIGQEEAKKHLAVAVYNHYKRISNPG
                     NDDLDLEKSNILLIGQTGTGKTLLARTIAKMLDVPFTIVDATVLTEAGYVGEDVESIL
                     TRLLQEADYDVEKAQRGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGSVV
                     NVPPQGGRKHPEQRMIAVNTKDILFICGGAFEGIEKKISQRLNTQVVGYGAEKKRNRI
                     DKDNLIKYVAPQDLRSYGLIPEIIGRLPVLTFLHPLNRQALRTILTEPKNALVKQYYK
                     LFELDGIKLKIEDEVLDYIVDTSIEYKLGARGLRSICEAIMVDAMYETPSQNRKTLII
                     KLDYAKKKIDKFTGSLAASA"
     gene            complement(16994..17677)
                     /gene="clpP"
                     /locus_tag="P5293_00540"
     CDS             complement(16994..17677)
                     /gene="clpP"
                     /locus_tag="P5293_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRR48454.1"
                     /translation="MAIEFEKFAKSRQGISSMTMHRFYSIYGAYINPTIIEERQLNVA
                     AMDVFSRLMMDRIIFLGVPIDDDVANIIQAQLLYLDSVDSKADIQLYINSPGGSIYAG
                     LGIYDTMQLVSSDVATICTGMAASMAAILLTAGAKGKRSALPHSRVMIHQPMGGAEGQ
                     AVDIEITAREITKIKNELYEIISFHSGKPVKVIEKDADRDYWMTATEAKAYGMIDEIL
                     IKSKKQISR"
     gene            complement(17689..19014)
                     /locus_tag="P5293_00545"
     CDS             complement(17689..19014)
                     /locus_tag="P5293_00545"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRR48455.1"
                     /translation="MRVTQKNIDDLNLVVTLNIEQGDYYDKQRKILNDFRRKAELKGF
                     RKGMAPMSLIEKLHGKSALLDAVNDLISEGLNNFISENKLKVLGEPLPNEKEQKPIDW
                     ENNSEFEFVYDIALSPSVKVELSNEDKIPYYEVVISDEEKKIYRSNFLKQYGQLGNVD
                     QVADDDFIVADLVQGDTKIEGTHISVRSIEDPEIKKLFIGRRVGDEFEIDVNKAFTND
                     ADRASLLRVKKEELTTVEPIFKVIVKEIKRFVDAEPTQELFDKVFGEGQVKSDEEFEN
                     RLIEKMKEEFARESDYRYMLDARDYLIEKAAIKLPEDFLKRWLYTANKEKFTMEDIEK
                     EFDLFLKDLRWQMIRQTILKEQGLTVTRELLDEQAKKIAAYQFAMYGIPNVPEEQLEK
                     YAQSLLANEKESGRILEKVEENLVLDFVKGVASLDKRQISSEELRKMTN"
     gene            complement(19077..19158)
                     /locus_tag="P5293_00550"
     tRNA            complement(19077..19158)
                     /locus_tag="P5293_00550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19122..19124),aa:Leu,seq:tag)
     gene            complement(19228..19995)
                     /gene="tpiA"
                     /locus_tag="P5293_00555"
     CDS             complement(19228..19995)
                     /gene="tpiA"
                     /locus_tag="P5293_00555"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRR48456.1"
                     /translation="MRKKIVAGNWKMNTTIKEAEKLASEIVELVDELETDVEIIICPP
                     FTHLSTVAKLIAKQHDEKEKIYLGAQNCADHASGAYTGEVSASMLKSVKCQYVIIGHS
                     ERREYYHENNESLYAKIELALKEGLTPIFCVGERLEERESNRHFEVVSEQIKDVLYKL
                     KGADASKIVIAYEPVWAIGTGKTATSDQAEEMHAFIRKIVADKFGDLAEVIPVLYGGS
                     CKPSNAREIFSKPDVDGGLIGGASLIANDFVEIIKSF"
     gene            complement(20128..21285)
                     /locus_tag="P5293_00560"
     CDS             complement(20128..21285)
                     /locus_tag="P5293_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRR48457.1"
                     /translation="MRRSAIRDLLSVANRPEIISFAGGFPNASTFPIEDLKKIMVEVL
                     ETEGASALQYGPTEGNQKLRELLAKRYCDNGCKVSAKNILITTASQQAIDLVSKIFID
                     PGDTVVCGLPSYLGALQAFWSYQAEPVGIKSDYDLEDTVKKLIAEGRKPKFVYAIPDF
                     QNPSGVTMTLEQRKYVLDVVKKYDLILVEDSPYRELRFDGEEQPMMYSLDDDERVILL
                     GTFSKTFLPGFRIGWAIAPEVIIDKLVVAKQSTDLCTTVFDQSVAAKYLEKGLFEKNL
                     QKTVALYKEKRDHMLACFEKYMPAGVTWTRPEGGLFLFVKLPEGFDARELFDIAMKED
                     VTFVIGEAFHCDGSGKNTMRINFSYMNSEKTEEGVKRLARAIERMMKEKGM"
     gene            21498..21938
                     /locus_tag="P5293_00565"
     CDS             21498..21938
                     /locus_tag="P5293_00565"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRR48458.1"
                     /translation="MSTVRITKEFHFEGAHALTGYDGKCRHIHGHSYKFYVTVKGEPV
                     NDTCNSKNGMVLDFSLLKQIVNEQIINLFDHSLILNKDAALSSEIEAAYQNVILVDFQ
                     PTCENLVLHFVELIRCKLPQNVSLQCIKMYETPSSYVEWMTDDN"
     gene            21928..24603
                     /gene="polA"
                     /locus_tag="P5293_00570"
     CDS             21928..24603
                     /gene="polA"
                     /locus_tag="P5293_00570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRR48459.1"
                     /translation="MTINNWFENMKKLYLIDGHALIFRSYYAFIRRPMINSKGVDTSI
                     LYGFTKTLLDLIIKEKPTHIAVAFDPPAKTFRHELYPEYKANRGETPEQVRGALEPLI
                     SILNAISIPVIMKPGFEADDVIGTIAKKAEREGFNVFMVTPDKDFGQLVSDNIVQFKP
                     GKNGADMETIGKEKICSIYNIDKPEQVIDILTLQGDISDNVPGVRGIGEVGSKKLVQK
                     YGSVENIYNKLDELPEKQQIALKEAKKHIELSKRLITIDTNVDIPWDEESLKLSTPNF
                     EELKRLFSEYEFSSLMRLIPKLEEVFELTGKPEDKSCKPQTELKAINYTIASADKVRN
                     EIFAKKELAIKLIKDRFIICCGELIQIVEETNIRQYKDILEDGSIVKCGYDLKSVMNK
                     LFEYPIILKGYMADIELMHYLLMPERAHKVEIIVPTYLGVSLDDQSNVIQGDLFDVNI
                     SLNEHSEKDLKETAVFLPLYHKLADELQKMSLDSLYNDIEMPLISVLSRMETTGVKID
                     TEILKKFSEKLNSELTAIEEEIRRIAQDPTLNVSSPKQLGVLLYDKLRLGAKNGKNTK
                     KNLSTDEETLLELENEHPVIPLILEFRNIKKLISTYADPLPQMISPKTGKIHTTFNQA
                     LTSTGRLSSVKPNLQNIPIRTERGKEIRKAFIPSYPEGYIMSSDYSQIELRLMAHMSG
                     DDNFIEAFRAGKDIHSATASKIFDIPEDQLTKEQRSRAKVANFGIIYGISAFGLAQRL
                     HISRGEAKKLIDEYFAKYPKVQAFINNTIEFAKKNGYVETLYGRKRFIPDINSKNPVV
                     RGLAERNAVNAPIQGSAADIIKIAMIRVDKRIIMEGIQSRMILQVHDELVFDVVPSEK
                     MLLTDLVKKEMESVALLSVPLTVDCKTGKNWLEAH"
     gene            24614..25183
                     /locus_tag="P5293_00575"
     CDS             24614..25183
                     /locus_tag="P5293_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR48460.1"
                     /translation="MDTINKFDEKELVRMAASGDQNAFLKLFERYIDQIYTYILTFIP
                     KPQDAEDICQETFHKAFKNIMYYQDEWAFSTWLYKIARNTAIDHYRKNSLTPYQSIDE
                     NREDASVFIDPSDVMPSPEENMINQQTFEMLIKAIKELDVKYRKVAELRFIHDYAYEE
                     IARELNLPLNTVRTQIKRARKLLAKKWKN"
     gene            25171..25815
                     /gene="rsmG"
                     /locus_tag="P5293_00580"
     CDS             25171..25815
                     /gene="rsmG"
                     /locus_tag="P5293_00580"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRR48461.1"
                     /translation="MEELIYKYFPRLSDQQRAMYKALYPLYEEWNSKINIISRKDFEH
                     FFLRHVLHSLAIAKFISFPNGARVLDVGTGGGFPGIPLAIMFPESSFYLCDSIRKKIT
                     VVGNVASTLNLNNVVTVNARAEELEGRYDFVVSRAVTALKDFLPWIWHKIDSGFFKGV
                     SRGVLYLKGGNLENEIALACSKMKISPSQIVEERIDQWFVEPDFAEKKLLFIKR"
     gene            25892..26044
                     /gene="rpmH"
                     /locus_tag="P5293_00585"
     CDS             25892..26044
                     /gene="rpmH"
                     /locus_tag="P5293_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRR48462.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMSTPNGRRVLSARRRRGRKKLTV
                     SSEEQF"
     gene            26119..27165
                     /gene="mqnE"
                     /locus_tag="P5293_00590"
     CDS             26119..27165
                     /gene="mqnE"
                     /locus_tag="P5293_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HRR48463.1"
                     /translation="MERDKIIKEALELYSTSDMNSISEAALKIRKAFNGNKVYYNNNF
                     HIEPSNICVHRCKFCSYRRDNSSQPGAWSMTLEDVRNYCREKYQKGMTEVHVVGSVHP
                     EKSIDYYVSIISLLRKELPDEVTIKAYTAVEIDDMARYSGQSVEYILNKLKDAGLSAM
                     PGGGAEIFDSDIRKQICPDKCNAERWIEIHRTAHKLGIRSNATMLFGHIENRRNRLDH
                     MLQIKELQEETGGFDAFIPLLFKSTNNQLSHLGEIGFVEILKTFAVARVVLDNIPHIK
                     SYWPMLGKDLSQLTLLYGADDMDGTINNSTKIYSMAGSHSNPEMSCDEIENMARECGF
                     IAIERDSFYNEVKK"
BASE COUNT         8870 a         5537 c         4835 g         7944 t
ORIGIN      
        1 aacactcata tctcctgtct ctctccaata ggcatatgcc agagatgatt tagtaccggc
       61 accatcagca tgcattacaa gacagtattc ggggtcatta ctaagcttat cagcaacaat
      121 tttgcaaaat gcatcaggaa acaatccctt atcatcatct tttattgcat tatgtacctc
      181 ctccttagat gaggatacac ccctttgcgc atacctttga gcctctgtac cgttgtccat
      241 atctatctcc agaaaagaag ttctctatat ttgggaaggg gccacaattc atcgtctatc
      301 agcatttcaa gcttgtcgac cactatccgt attttagtca tcgttggtaa aacattgtca
      361 tagtatagtc ttgccctttg agccatatct tccactttgt tagcaatttt tcgatcctca
      421 ataagctcct tggtattctt tcgtaaagtt tcaatgtatc cggatatctt tcgaattgtc
      481 ataagctgat tttcagacat ctgacgatat tcttcctcgt caaaaagctc cttgatacct
      541 ctgagatttt ctataagagt gttctgaaat ttgatggctg taggaattat gtgattttgt
      601 gtaagatccc caagaaccct tgcttcaatt tgcagttttt tgacaaatgt ctcattcatc
      661 acctcatatc tggcttcaac ctcacgtctg gtcaatactc cagtctcttc aaaaagcctg
      721 acactgttct cttccagata agtgttaaac gcattcggta aatccttaat accgcgaagt
      781 cctctccttt cggcctcttg atgccattca ggactatatc cgttgccctc aaacaatatg
      841 tttctcgatt ctataatgaa ttgtcttata actttaagaa gagcctcatc cttttctata
      901 ccatctttca tgtacacctc aacaagaaat ttaaatctcc ttatctgctg agctacagca
      961 gcattcaagg ggtaaagggc tgccgcaaca ttaactgaag aacctacggc tctaaattca
     1021 aatctattgc ccgtaaatgc aaaaggagaa gttctgttcc tgtctgtatt gtcaggcaga
     1081 atttcaggta tcttattaac tatatccagc ttgtgatgta aatcctcaat cgactcctct
     1141 ccgtttgaca ttttttcaat agaatcaata acagacgaaa gaagcttccc tataaaaata
     1201 gacattactg caggaggagc ttcatctcct ccaagtctgt aagagttgtt aagtgatgcc
     1261 acaccagcca tcaacaaagg atgatgttca taaacagccc ttattacaga aacgaaaaac
     1321 gagagaaatt gcaaatttgt tcttggtgtt ttaccagggg ccagaagatt gactccattg
     1381 tttgtaataa gtgaccagtt acagtgtttc cctgaaccgt taatacctga aaaaggtttc
     1441 tcgtgaaata taactttaag tttgtgtctc ttggctattt tcctcatcag tatcatcatc
     1501 atgagattgt gatcaactgc gaggttagcc tctgtaaaat atggggcaaa ttcaaactgg
     1561 ttaggtgcca cctcattatg tctggttttt agaacaaccc ccaatttata tgcctcagtc
     1621 tcaaaatctt tcatgaaagc cataaccctt gaaggaattg ctccaaaata atgatcctca
     1681 agctgctgat cttttgcaga ggtatgtcct aaaagagtcc tgttgcacat catcaaatcc
     1741 gggcgtgcac ggtaaagagc ctcatcaacc agaaaatatt cctgctcaat accaagcata
     1801 acattaactc tggttgttct gtgatcaaag agtttacaca agtctgtagc agctctgtct
     1861 aaagcctgaa gtgatttaag atgaggagct ttatgatcaa gagcctctcc tgtataagaa
     1921 acaaatacag ttggaataca taatgtctga tccattataa atgccgggga ggtaggatcc
     1981 catgctgtat aacctctcgc ctcgaatgtg tttctcagac ccccatgagg aaaactggat
     2041 gcatccggct cctgctgaac caaagcatct ccccggaaat tctcaaaaac tccaccattc
     2101 ttcgaaggat cgacaaaagc ctcgtgcttc tctgccgttg catctgtcaa agggtgaaac
     2161 caatgtgtat agtgagtagc tccattttca acagcccatg acttcatgcc tgacgctatt
     2221 tgattggcaa tacttctgtc tatttttgtt ccttcgctta tggcctgctg aacagcttca
     2281 aaagcctctg cagaaaggta acgtctcatc ttttcgacat caaaaacctt ctctgcgaag
     2341 aactccgaaa ccggtctgtt ttccacataa aaccgctcag ccttatggct ggcaaattca
     2401 tctagagctc gttttctgaa actactcatt aagcaatagt aaaagtttat tataattaat
     2461 tacagcgtca aatgtagcta tttttcgtta agcatttatt aaaattatta tttttgcttc
     2521 aataataaac cttttatcat gaaaaaacca tccttatttc tactgatatt cttgatattg
     2581 gctacatctt tcaatttgtc agccaagaac agaattgtcc aaaaatgtgt aaatcagatg
     2641 aaaaaagaca ctttgtttag caactccgtt gttggaataa tggtaatgga cagcaagggg
     2701 aagtgtgttg catcatggaa tcctgattct cctctgctta ctgcatcaac tatgaagact
     2761 atctcaacag gactcgcatt acagcttctc gggcctgact atcgatttaa aaccagaatc
     2821 ggatattccg gacaaatcga gaatggcgtt ctaaacggag atctttatat tgttggagga
     2881 ggagatccga cgcttggttc aaaagacact gtagcaattc ctgtagatac agtcttctcc
     2941 caatggtata aatttatcag ggctgcaggc attgttaaaa taaacggatc aatagtgggt
     3001 gacgatcgtt tttttgaaga agaagctttg cctgacacat ggtcatggag taacattggg
     3061 gcatcatatg gaagcgcagc gtccggtctc tcattctgcg aaaacataca atattttact
     3121 ttcatccctg gagaaaaaac tggcgaacca gttgtaatga aagatgtata tccatccatt
     3181 ccgggtatgg aataccggaa catgcttaaa acaggcattt ccggtagtgg agacagaagt
     3241 tcttattatg tatcagatct tgcaaaagtc ggagttttca aaggcactct tggagctggt
     3301 aaagaaagtt acaccgttga ttgttcaagt aaatttccgg ctcttatgtg tgcagaggag
     3361 tttcgacttt tccttattga taaaggaatt gaaagcaatc ctgatgtcaa agatgtaagg
     3421 tcttttgatg caacagatca ggatcaatta aacattatag gagagactct ttctccaaga
     3481 ctgatatcca tagttaatgt caccaataga ataagcaata acttttatgc agaaactttt
     3541 ttcaaaatga taggcaaaca gttgaccggc aacggttctt atgattcttc agtagtggca
     3601 gcgaaaagag aacttgccaa aatggggtta tctctcaaag gttatactca agttgatgga
     3661 agtggcttat ccagacaaaa ttatgtttct ccaagatttt tctgcaaata tttcgcaaga
     3721 atgaaagaaa tgaataactt tgctgaattc atcggttctt ttccacaacc tggcggtcca
     3781 ggaaccttga aaagtgtact gaaagatgca gatagcgtta ccaaatcaaa gattcatgct
     3841 aaaagcggat ccctctcaaa tgtcaagtgt tacgcaggtt atgttgaaaa gagagatggt
     3901 gaatatgatt attttgcaat attgacaaac aactaccaag caagaacttc cgagatacag
     3961 gctggggttg agagattcct cggagcgctt gttgatatta aagagacaag taaaaagtaa
     4021 aaacattata atataaaaac gacaaataat gcttacatta tctaaaaagt ctgaaatcat
     4081 gccggcttct cctatcagaa aactggtacc ttacgccaat gaagccaaaa aaagaggaat
     4141 taaagtttat caccttaaca tcggacaacc tgatattgag tcgccaagag aggctctcga
     4201 tgctgtaaaa aacaacactc ttaaattagt tgagtatcct gactcaaagg gtaacgaatc
     4261 ctacagaaaa ggtctagtaa agtactatga gagcataaac atccatgtaa ccgaaaacga
     4321 tatcaatatc actacaggcg gaagtgaagc tcttcaaatt gcacttgcag ttacctgcaa
     4381 tcctgatgat gaagttatag taatggaacc gttctacaca aactacaact cctttgctct
     4441 tcagaacaac gtagttctta agcctatcac caccagtatt aaagatggat tcgctattcc
     4501 cgatccttct gagtttgaga agagaattac tcctaaaaca aaaggtatta ttatctgcaa
     4561 tccaggtaat cctaccggga cactctattc aaaggagtcc attctaaaaa taggtgaaat
     4621 tgctaaaaaa catgaactgt ttatattttc tgatgaagtt tacagagaat tctgctatac
     4681 agatcagcct catttctcag ccatgcatat ccctggcctt gaagaaaatg taattcttat
     4741 tgactcagtg tccaagagat acagcctttg tggagttcgt attggtgcaa tagtttcaaa
     4801 aaacaaggca gttatgaatg ctgttctccg ctttgcccag gcacgtctct gttctcctgc
     4861 ctatggacag attgctgccg aaggagctct tgctactcct aagtcttatt tcgaagctgt
     4921 acgtgcagaa tacatcaaaa ggagagactt cctgattgaa gcattaaaca agatggaagg
     4981 tgtatattct cctatgccta tgggagcttt ttacacaatt gcaagccttc caattgacga
     5041 tagtgacaag tttgcacaat ggttattgga agattttcaa tacgaaggcc aaactgtgat
     5101 ggttgcccct gctgcaggtt tttacgcaac taaaggcaag ggaactaatg aagtacgcat
     5161 tgcctatgtt ttaaaaattg acgatctcaa agctgcgatg aaatgtcttg aaattgctct
     5221 aaaacagtat cctggaagga caactaaata aaacaggcaa ttctgcccaa aatcaaaaaa
     5281 atagtcacaa gaaagaaata cagtctttgt ttgtgactat tttcattttt ccctgttgat
     5341 tcaactatta aaacagaaga aggaaaagaa aatatttgca tgattctgcc acttgaatca
     5401 ggatacagaa taataattcc tgacaaaaaa aacataagta tgatcaatct gacaaaagag
     5461 gcactttgaa taattttgaa cctgttaatc ctgttaaaaa tgtcacgcaa tgataatatc
     5521 agcaataata acagaaatat tgtaaaaaaa agatgtgata cactcctgat atctggcttc
     5581 gctatgtttc caattttaag atgatataga tactcctctg caaaaatatg agcatcatta
     5641 aaaaatataa atcttaaaga aaaaataata cctactggaa taagaaaagc caataagatc
     5701 agcactaatc ttctcattga gataccggtt tctttaatcg agaaaaaaaa taccagaggt
     5761 ataatatata tcataagagg ctcaaaaagt gaggcgattg tggcaagaaa aacagccagg
     5821 aaggagtatt gctcattatt tcttattttg agcgatgtgt atattgacca tattatcaaa
     5881 acaacagcta ttgaacctcc tgaaaagagt gatgtatgat aggatgaaaa agccagaact
     5941 agataggtta agggcaaagt caatccttct tttccggtca ttaaatacct ctcgttgaaa
     6001 atataaagtg agatcgtaga aattataaga aaaaatgctg caagaagatg agaatataaa
     6061 gttggaatat cctcaggctc tataaatccg gcactcaaag ggatccatct ctcaaaatta
     6121 tcatccggaa gctttaagga ggtaaaaacg acaaaaaaca gcaacaaaat taaatacggc
     6181 aacgaaactg caaaattata tcttttgtcg tattgcatct ttacaaaact attttgaata
     6241 tctcatcaag tcgagaccta tatcctttct aaaataaata tcattaaaac taatcttttc
     6301 tatctcttta tacaaaattt gacgacattt atgtatatcc ggagctaaag ttgtaagagc
     6361 caatactctt ccaccagaag ttacaacatg accatctgaa acttttgtac ccgaatgaaa
     6421 aagtaaaata tcattatcct gctcaaattt ttcaaggcct ttaattagat agcctttttt
     6481 atagtcttcc ggatatcctc ctgatacagc aactatagtc atggcaactc ttgaatctgt
     6541 aacaatcttt tcgcccgaca attttccctg agctgcagca accaggtgag acagaaaatc
     6601 actcttaatc ctcggcaata ctgcttctgt ctcaggatct cccatcctga cattatattc
     6661 aacaacatac ggatcaccct cgcaattcat aagaccaata aatataaatc ccttgtaatc
     6721 aatcttatcg cgcacaagcc cttgaattgt gggttttatt attctctctt caacctttct
     6781 cataaactct tctccagcaa acgggacagg actgacggct cccatgccgc cagtgttgag
     6841 acctgtgtca ccatctccaa ttcttttata atccttggcc tccggcagaa ttaaatattc
     6901 ttttccatca gtaagtataa agaccgagag ttctatcccc ttaagaaatt cctcaatcac
     6961 aactttacgg cctgccgttc cgaatttccc attaaacatt gcctgcaatt ctctcctggc
     7021 atcgtctata ttactatgaa taacgacacc tttaccggca gcaagtccgt ctgccttcag
     7081 cacgtaaggc gcactgagtt tctcgagaaa atagtctgcc tcatgcatag atgaactgtc
     7141 aaaacacatg taggaggcag taggaatcga ccatttttgc ataaaattct ttgcaaattc
     7201 tttgctacct tcaagcagcg cccctttttt gccaggccct ataaaaaggc aatttttaag
     7261 ttgatcatga gattcaaaaa aatcacgaag accgtttacc aaaggctctt ctggaccaac
     7321 aacaaccaac gtgatgttat gctccatcac aattagtttt atggcctcaa aattttttat
     7381 gtcgatatca atgtttgtcg caattaatgc tgtacccggg ttacccggag cacagaacag
     7441 tttttcgaga agaggacttt tgcttatact ccacgctaac gctgattctc tccctcccga
     7501 tccaattatt aggactctta tcatttgtca actctatttt tatcaatcag gagcaaaaat
     7561 aataataata aactaacttt gtttttgaaa ataatagcaa tgaggcgttt ttacagcata
     7621 tttcagatta tagtcattat cattacaaca acttcctgca gtaaaaaaga tataattcca
     7681 agaaaagaat tgccacagat acttgcagag atgtacattg cagatcgtgc cctactgagt
     7741 gatcctgcca agttaagaca ggccgattct cttctggttt atgaacccat tctgaaaaaa
     7801 tatggctaca caaccaaaac actaatcaat acttttaatt actatttacc aacgccagcc
     7861 aagcttaaga gttcatttat ggctgcaaga gcaattcttg aaaaaagact tgtagaagtt
     7921 cagaatcaga ttgccctcat gcaaaaaaga gaaatagtat tcgccaccgt tcacaaacta
     7981 atacgggaag cagattcttt aagatatatg gattcatatg aaagatcttt aagatggctg
     8041 ttagcacctg aactatttcc aaacagacct gtttatatcc cggactccct tatatcacgt
     8101 tatgaacatc cgaaaatgac aaattggtgg ttgaacaatt taaaaacaga aagcagacat
     8161 ttctaccagt atgagaacaa tagcagcaca atacctcttc tcaatagacc acagcccaat
     8221 caggagagga tatctctgcc tgaacactga cggagtaatt gtagaagttg ggcagttaga
     8281 agaaaatgag gagcgagaaa gtacagaatt ttatgatggg attatatgcc ccggctttgt
     8341 aaacagccat tctcatattg aactttctca cttaaaagac catttcagtg agggcagcgg
     8401 catggccggt tttattcgtc agatcaatgc attgagagaa tgttccagtg tcgaggaacg
     8461 tgtagccgca atcgaaagag aaatgaaaca gcttttcgaa agtggggtct ctgcaatggc
     8521 agatataagc aattgtgatg aaagttttag aataaaatct gtttctcaat tatatacacg
     8581 cacatttatt gaacttttcg gctcagaacc tgaagagtgt gaaacaataa tgcacaatgg
     8641 ctttaaactg cagcaaatag caaagtccta tgaccttgat gctgcaataa caccacactc
     8701 atgctacaca atgagtccgg aattaaacaa agctgccatc aaagccgccc tcaaagaggg
     8761 ttttctctca tatcacaatc aggaaagctg ggaagaggaa gaacttataa aaaatggcac
     8821 cgggccattg gccgaagaat ataagagacg gggattgagt acaccacctg ttattggaaa
     8881 gcctgcactg ttctactttc ttcaaaatct ctcagaagta aggaatttaa gcaaagagac
     8941 tgtaaaggaa catattcttc tcgttcacaa cacttttacc aatgaagaga gcattgacgc
     9001 tgccatgaca atgatcagca atctgttctg ggcaatttgc cctctatcca atatttttat
     9061 acacagatgt ctgccacctg tcgagctcat gagacgcaaa ggatgcacga taactcttgg
     9121 cactgacagt ctttcaagca acaaagttct ctcaattgtt gaggagatca aagtcattca
     9181 taattacttt ccaaacatct ctcttgaaga aatacttaaa tgggcaactc taaatggagc
     9241 tatgtttctc ggcaaagaaa aagagttggg aagtttttcc acaggtaaaa aaccaggagt
     9301 ggtgctcata gacaacatcg actggaataa aatgcaactt actgaaaata gccactccaa
     9361 aagaatagtt taacatattc aaataattat gtccacaata cttttcaatg aaattattta
     9421 tggtcctatc aaaagccgcc gacttggttc atcgcttggt gtaaatcttc tacctccaaa
     9481 cggtaaatgg tgcaattttg actgccttta ctgcgaatgt ggctttaaca aagacgggaa
     9541 agacaatcga caaatccctg ccagagaaga tgttcgcaag agtctcgaaa gagttttgtc
     9601 aaacctttca acaaaaggag acagaatcga cagcataaca ttttccggta atggagaacc
     9661 cacaatgcat ccggactttg cagctataat tgaagatacc atttctctta gaacaaaata
     9721 caagccagaa gccaaagtgt ctgtgctttc aaatggaagc ggaatagcaa gaaaagagat
     9781 tgtagacgct cttctaaaga ttgaaaatcc tattttgaag atagattctg cttttgacag
     9841 aaccattctt cttatcaaca gaccagcata tgcatattca attagaaaat tgaaacatga
     9901 ccttcaacca cttagcgaca gatttgttct tcaaaccatg tttctaagag gggaatttga
     9961 ggggaagaca attgacaaca caacacctga agaggttgaa gcatggcgtg ctttagttga
    10021 tgaaataaaa ccaagagaaa ttatgattta taccgttgac agagagacac ctgctaagaa
    10081 tttatgtaaa gtctccgttg aagagatgaa caagtttgtt gcgcctctga tagagaaagg
    10141 ctataaagta aaagttagcg gataagatga gaattgtagt ccaacgagtt cttgaagctt
    10201 ccgtcaaagt tgacgggata tgtacaggga agattggcaa agggttgctg ctttttgcag
    10261 ggtttgaaga taacgacaat gattctgata ttgcttatgc agcaaaaaaa gttgtaaatc
    10321 tccgtatatt tgatgatgat aatggagtaa tgaaccattc tattcttgag aagtgcggag
    10381 gtatactttt gattagccag tttactcttt ttgcccagac acgcagaggc aatcgtcctt
    10441 catacattat ggccgccaag ccggaaaagg caattcctct atatgaacaa tttagaaaag
    10501 aactggaaaa gacattgggg aaaaaagtcg aaactggtgt tttcggtgct gacatgaaag
    10561 tttctcttat aaatgacgga cctgtaacaa tctggatcga cactaaagtt gacaaaaaca
    10621 gttgaaaaat ggaaaatatt aaaagagaga tatcaaaaat tacagacaac attgataatt
    10681 ggctcaataa agaggcttta agaaaataca taggactaat tgatcttaca tccctgaact
    10741 ctgcagacac atcttccaaa atattgggat tggtagagaa agttaacaac tttaatattg
    10801 actttcctaa atatccttct gtagctgcaa tgtgtgtcta tccaaatttt gcaaagctgc
    10861 ttaagtccag tctcaaggtt aagaatgtta aaatcgctgt tgttgcgggt gctttccctt
    10921 cttctcaaag tttcaaagaa ataaagaccg cagagtgcag gatggctgtt gaaaacggcg
    10981 ctgacgaggt tgatatagtc cttgctctta acaagtttct agataaaaaa tatgaggaga
    11041 caaaatcaga aatcagagac attaaggcag ttattggaga caaacatctg aaagttattc
    11101 ttgaaaccgg cgtacttaag gaggaacatc ttattgatta cgcagctgaa attgccattg
    11161 aatctggtgc tgattttatc aagacctcta caggtaaaat tgaacctgcc gccacacctg
    11221 aagcagcata tatcatgtgc aagcgaataa aagcatatta cgaaaaaacc ggaaaacgca
    11281 tcggatttaa gccagccgga ggtatatcaa cacccaaaga ggctgctctt tactttgcca
    11341 tagttgaaaa tgttttagga gacgaatggt tgacaccaca attatttcgg tttggagcaa
    11401 gccgtttggc aaacaacttg ctaacagagg tagaaggaaa ggtaattaat tatttttaat
    11461 acacagacaa tgaaaaagac aatagttctg ttaactgcat tactcataac tgttagtgca
    11521 gtcgcccaaa actatcatag ggcagtcagt gccagatttg gcacaagcgt aggagtttcc
    11581 tttaaacaat ttgtctcttc tgcggcagca tttgaactta tgagctctgt tgatctggtt
    11641 gagaataaca accaaaaact tattttttct gcctacggtc aatatcatta tgacaaactt
    11701 ggtgtcgatg gactttcgat ctttggaggt cctggaacat cagctggtct tttcatctca
    11761 gggaaatatc aggataaggc agtactctct tttgacataa ttaccggtgt tgagtataaa
    11821 cttcaagaac ttcctcttat tttggcattt gactggaacc caaaactaca aatgattact
    11881 gacgcaggca ttaaacctgc caatttcggt gtaacactca gatatacatt ttaacgcata
    11941 aatcatgata aaaaattaca ttaaggaaaa cagcgacaga tttctcaatg agttattcga
    12001 tctactgaga atcccatcgg taagttccat ggcggagcac aaacaggata tgcaaaaagc
    12061 tgctgaaaag tatgttcatt tgcttaaaga ggccggtgca gacaaagttg gagtttacca
    12121 gacaaaaggt catcctgtag tttttggaga aaaagttatt gacaaatctc ttcctacaat
    12181 tttggtgtat gcgcattacg acgtacaacc tgcagatccc atcgatcagt ggtctacacc
    12241 tccatttgag cctgttatta aagatggagc tatttacgct cgaggtgctg acgatgacaa
    12301 aggacaaggt ttcatgcatg taaaggcttt cgaatatatg gtaaggacag gccagttaaa
    12361 atgcaatgtc aagtttatca tagacggaga agaggaaatt ggttctccaa gtctctctga
    12421 atgggctgca gaacacaaag agatgcttgc atgcaatgat attcttgttt ctgatactac
    12481 aatgattgac gaaaaggtac cttctttgaa tgttggaatg agaggactca cctacatgga
    12541 ggtggaagta acaggcccta acaaggatct tcattccgga cattatggtg gagcaataag
    12601 aaatcctgcc aatgctcttt gcagcatgat agacaaatta atagacgaca agggcagagt
    12661 taccattgaa ggcttttatg atgatgtggt tgaacttaca aaagaagaga gagatatgct
    12721 tgcaaaagca ccttttgata tagaagaata taaacgcttt cttgatattg acgacgtggc
    12781 cggagaagaa ggctacacta caatggaacg gacaggtatt cgtccgtgtc ttgatgtcaa
    12841 cggcatatgg agtggctata caggagaggg agctaagacc gtcatacctt caaaagctta
    12901 tgcaaagata tcaatgagac tcgttcctaa ccaggatcat aataaaatag ccaagctgtt
    12961 tgaaaatcat tttcttaaac tcgcaccgaa aggagtgaaa gttaaggtta agcatcatca
    13021 tggtggacag ggatttctat gcccaatctc ctcaaaggta tatcaatccg catcgagagc
    13081 aattacagaa gtttacggaa tagagccagt gccctccagg ggaggtggca gtattcccgt
    13141 tcttgcagaa ctgcaacaaa tacttggagc caatcctctg ttgatgggtt ttggacttga
    13201 aagagacgtt attcattcac ctaatgaaag ctatctacta agtcaattct ataagggaat
    13261 tgaatctatt gctctttttt acaaatatta tactgaataa attgtaatca aatttaaaat
    13321 atttcatata tttgtagcat aatttatctc gtatacctcc aattattgga ggtatacgtc
    13381 tttttatatt atattacaaa tagttatgaa aattgggatt attggttttg gcagtatggg
    13441 caggatgctc atgcacaagt ttttggaaac aggagctatc atgcaggaag aactttatgt
    13501 ttcaaatcgc actacagaaa agctttcaga aatttccttt ttctacccca aagtcaatat
    13561 ctgtaataat gaatccctcg ttaaatcatc agaccttatc tttatttgtg taagaccagc
    13621 ccaaatgaag gatgttgcag aaattattac accacacatc caaaatagac aacatatatg
    13681 cgccgtcaat gcttgtataa gattcaacca gcttgactca atatttccag gcacttcttg
    13741 ttcgattgtt attccaagcg taacaggaga ggtcaataaa tctgttacac ttgcctcatt
    13801 taactcctat gtttcaaaac atgaggtcga cttgattaaa tcacttctta gctctttcag
    13861 ctcagtgtta gttatccctg aaaacgaact aggaatttgt actgatctga caagctgcat
    13921 gcccggcttc atcgcttcaa tatttgacat tattgttgac gaagcttctc aacattcatc
    13981 tttgtctaaa gatgaaattc acagtatgat tattaccacg attctgggaa catcaaaact
    14041 ttgtgttgat cagaaaatgt catttgacaa aattgtcgaa agagtcgcta caaagggagg
    14101 aatcaccgaa gaaggagaaa aagtaatccg gtctcgcttc ccagagatag ttaaaagcct
    14161 ttttcaacat acaagagaaa agagagaaaa cactattaat aatttttcat ttgaacctaa
    14221 atgtgaaaag tgtaagaaac tataatactc aatattagta attattgctg tctagattgt
    14281 gcagggcata acaataggct ccaagcgaaa tggctttact tgcccctaac ttcgaaatca
    14341 tcactcccgt ccttttttca gggtcgtaaa taactttgtc gttacatcca taaaccttca
    14401 actcacgact ttggcctctg gcaaattgtt caaattcctc ttcagaatca agattgtata
    14461 cactcatttg aagacgacca acattctctc cactaagagt tttaaggctg ctcctcatct
    14521 cccgcaagag agagggcatt ataaacttgc tggcagcagt aatacctccc ccaatcacaa
    14581 ctaagccgtc aattagagaa atcgctgtaa caatagcatc tcctgctact tctccaaaaa
    14641 gctcaaaagt tttgatagca gcattcctgt caccttctct tactccgttt gctattctga
    14701 atatatctaa aggctctaaa tcatgaacca gctgatttgt caattctcca tagacccgtt
    14761 taatagctct tgcagaaact ccctcttcaa caataatatc aggcataagt ttgtgtctga
    14821 gacaaaaagt ctctgtacat gagttgtccc caatatgaag tgcgccatta aatgaaattc
    14881 caaatccaaa accagtgccc catgtgtaac ctatcagatt attgtactgt ttgatgctcc
    14941 caacctctcg caaacgtttg ttaacctcag gcaaagctcc agctattgcc tcaccatatg
    15001 tgaagagatc cgcgttatta tttataaaaa caggtaatcc aaattttttc tcaagaaacg
    15061 gtcccaatgc aacaccgtcc ctgaaagatg gaaaattagg caaatagcct cctataattc
    15121 cattcttgta atcagcaggg cccggaaaag caaaactaat tgccacaggc gtttctccca
    15181 aagaccttat aactgcctca aatcctttaa caagaattgc aagacaatta tctaaatctg
    15241 aagcatttgt ttgcaatgaa ataggtctca ttacataatc attaccctgg acagcactga
    15301 aaacaaaatt tgatcctcct gcatctaagg taactacaac acggctatca ttttcgtaca
    15361 tacttatatt tgttaactta tcattaaaga tttaaacaaa tataaacaat aatgtagaaa
    15421 atgatctcca aaacctaata aaataaagcg atagcgaaga atttacttta aaataatttg
    15481 ataaaataaa aatagtacta tcttcgtact gaggctatca tataaaaaag attaaaatag
    15541 tatgaaatgg gtcacaatac ttacagctgc ttatccccaa gatatactga ttcctcagtc
    15601 aaatcttgag gcacggggaa ttcacacttt tctaaaagat gaatactata attcgatagt
    15661 caacttttac cctaactcta acgtaaaact tcaagtgcca gaagatgagt acgatactgc
    15721 tgctgagata cttaaagccg caggctacga aatcatgccg aagccgccag agagccggta
    15781 aacttgtcaa tcttcttttt agcgtaatcc agcttaatta tgagagtctt tctattttga
    15841 gaaggagttt catacatcgc atcaaccatt atagcctcac atattgaccg caaacctctc
    15901 gctccaagct tgtactcgat tgaagtatcc acaatataat caagcacttc atcttcaatt
    15961 ttgagtttga tgccgtcaag ttcaaaaagt ttgtaatatt gtttgacaag agcattttta
    16021 ggctccgtaa gtatggttct taaagcttgc ctgttaagag gatgaagaaa tgtaagaacc
    16081 ggaagtcttc caataatttc gggaataaga ccgtaggatc ttaaatcttg tggagcgaca
    16141 tatttaatca aattatcctt gtcaattcga tttcttttct tctctgctcc ataccccaca
    16201 acctgagtat ttaaacgttg ggaaattttc ttttcgatcc cttcaaatgc tcctccacaa
    16261 ataaaaagaa tatctttagt attaacagct atcatccttt gttccggatg cttgcgacct
    16321 ccctgaggag gcacattgac tacacttccc tccaataact taagcaaagc ctgttgaacc
    16381 ccctctccag aaacatctct tgttatagat ggattatcac tcttccttgc aattttatca
    16441 atctcatcaa taaaaacaat acctctttga gccttttcaa cgtcgtaatc ggcctcctga
    16501 agcaatcttg taagaatgct ctcaacatct tccccaacat atcctgcctc agtaagaaca
    16561 gtagcatcta caatggtaaa cgggacatcg agcatcttag ctatagttct ggctaaaaga
    16621 gttttacccg ttcctgtctg accgatcaac aatatattgg acttttccag gtccaaatca
    16681 tcatttccgg gattggatat cctcttataa tgattgtaca cagctactgc aagatgtttt
    16741 tttgcctcct cctgcccaat cacatactga tccaaaaaac ttttaatttc caggggcttc
    16801 atcaatcttc tctgatgatc ttcctcctgg ctaccctttg gctttcttgc tttcatgact
    16861 tcccgcgcat aatcgacagc ctgctgggcg cagtcactgc agatacaagc aatttctcca
    16921 actatcaaaa catctacttc atccttgtct ctgccacaaa atgaacagta atcattaaat
    16981 tttgatgtca tttttatcta ctgatctgtt tttttgattt aatcaaaatt tcatctatca
    17041 taccataagc tttagcttct gtagctgtca tccagtaatc cctgtctgca tccttttcaa
    17101 taactttcac aggtttgcct gaatggaatg aaattatctc ataaagttca tttttgatct
    17161 ttgtaatttc ccgcgctgta atttctatgt ctacagcttg tccttctgct ccacccattg
    17221 gttgatgtat cattactctt gaatgaggta atgccgacct tttacctttt gcacctgcgg
    17281 taaggagtat tgcagccatt gaagcagcca ttcccgtaca tatcgtagct acatcagaac
    17341 ttacaagttg cattgtatca taaattccaa gccctgcata tatcgacccc cccggtgaat
    17401 tgatataaag ttgtatatca gcctttgaat ccacagaatc cagatacaga agctgagcct
    17461 gaattatatt ggcaacatca tcatctatcg gaactccaag aaaaatgatt ctgtccatca
    17521 tcaatcttga gaaaacgtcc attgcggcca cgtttaattg acgttcctca atgattgtag
    17581 ggtttatgta ggcgccgtaa attgagtaaa acctgtgcat tgtcatgcta cttatacctt
    17641 ggcgcgattt cgcaaatttt tcaaattcaa ttgccattat tcaaaatttt aatttgtcat
    17701 tttacgaagt tcttctgaag atatttgcct tttgtcgaga gatgccacac cctttacaaa
    17761 atcaagaact agattctctt caactttttc aagtattctc ccagactcct tttcattggc
    17821 aagaagagac tgtgcatact tctccagctg ttcctcaggc acatttggaa ttccgtacat
    17881 agcaaactga tatgcagcta tcttcttagc ctgttcatcc agcaattctc gagtaacggt
    17941 aagtccttgt tcctttaata tggtctgacg aatcatctgc caacgcaaat ccttgagaaa
    18001 aaggtcaaac tccttttcaa tatcctccat cgtaaacttc tccttatttg ctgtatagag
    18061 ccacctcttg agaaaatctt caggaagttt aattgcggct ttttctatca aataatccct
    18121 tgcatcaagc atataacgat aatcactttc ccgagcaaat tcttctttca tcttttctat
    18181 aagtctgttt tcaaattcct catcactttt aacctgacct tctccaaata ctttgtcaaa
    18241 cagctcttgt gtaggttctg catcaacaaa acgttttatc tccttaacaa ttaccttaaa
    18301 aattggttca acagtagtga gctcctcctt tttaactctc agcaatgatg ctctgtctgc
    18361 atcattagta aaagccttgt taacgtcaat ttcaaattcg tcacccactc tcctcccaat
    18421 aaacaacttc tttatttcag gatcttcaat ggatctcacg gatatatgtg tcccttcaat
    18481 cttagtatct ccctgaacaa gatctgcaac tataaaatca tcatcagcca cctggtctac
    18541 attgccaagc tgtccgtact gcttgagaaa attacttctg taaatcttct tttcttcatc
    18601 tgaaattaca acctcataat aaggaatctt gtcttcatta ctgagttcca ctttaactga
    18661 aggagataat gcaatatcat acacaaattc aaattctgaa ttattttccc agtcaatagg
    18721 cttttgttct ttctcgttag gaagtggttc tccaagaact ttaagtttgt tctcagaaat
    18781 aaaattattc aagccttcag atatgagatc attgacagca tccaacaagg cagattttcc
    18841 atgcagcttc tctatcaggc tcattggagc catgccttta cgaaagcctt tcaactcagc
    18901 cttgcgtcta aaatcgttaa gtatttttcg ctgcttgtcg taataatctc cctgttcaat
    18961 gttaagtgta acaacgagat tcaaatcatc gatgtttttt tgtgttactc tcatgtatat
    19021 ataaattatt ttgttatcaa caaaaaacag ataccccaaa ggctatctgc tttcagtgcg
    19081 ggcaaaggga ctcgaacccc cacgccttac ggcaccagat cctaagtctg gcgcggctac
    19141 caattacgcc atgcccgctt tgagggatgc aaaggtaaga caattttctc acattacaac
    19201 agttataaac aatgagactt acgactttta aaaagatttt ataatttcca caaagtcatt
    19261 agcaatcaaa gatgccccac ctataagccc gccgtcaaca tcaggtttgg aaaatatctc
    19321 acgagcattt gatggtttac agcttccacc atacaaaaca ggtattacct cagccagatc
    19381 tccaaatttg tcggcaacta tctttctaat aaaggcatgc atctcctcag cctggtcaga
    19441 ggttgcagtc ttacctgtac ctatcgccca taccggttcg tatgcaataa ctattttact
    19501 cgcatctgca ccctttaatt tataaagtac atccttaatc tgttcggaaa caacctcaaa
    19561 atgtctgtta ctctctctct cttccaacct ctctcctaca caaaaaatag gtgttaaccc
    19621 ctctttaagt gcaagttcaa ttttagcata aagggattca ttattttcat gataatactc
    19681 tctcctctct gagtgaccta taataacata ctggcatttc accgatttta acatgcttgc
    19741 agaaacctct cctgtatagg cacctgaggc atgatcggca caattttgag cgccaagata
    19801 aatcttttcc ttttcgtcat gctgttttgc aataagtttt gccacagtgc tcaaatgagt
    19861 aaatggagga catataatta tttccacatc tgtttcgagt tcatctacaa gttccacaat
    19921 ttcactcgcc agtttttctg cctccttgat agtagtattc atcttccagt tacctgcaac
    19981 aattttcttt ctcatatcca aaaaaattaa taattcaaca acaactgcaa aattaataaa
    20041 taataaaacc cgagaaaaat taaatcaaaa gaggggtgtg taacatattg ccacataccc
    20101 ctctatgctt aaaattgatc taaaacacta catgcctttc tctttcatca tcctttcaat
    20161 agctcttgca agtcttttta cgccctcttc tgtcttttca ctgttcatgt atgagaaatt
    20221 aatcctcata gtattcttgc ctgaaccatc acagtggaat gcctctccaa taacaaaagt
    20281 cacatcctct ttcatggcaa tatcaaataa ttctcttgca tcaaatcctt caggcaattt
    20341 gacaaaaagg aaaagtccac cttctggacg tgtccatgtc acacctgccg gcatatattt
    20401 ctcaaaacat gctaacatgt gatctctttt ttctttatag agagcaaccg ttttctgaag
    20461 attcttttca aaaagtcctt tctcaagata ctttgctgca acagattgat cgaaaactgt
    20521 tgtacaaaga tcggttgact gttttgctac tacaagtttg tcaattatca cttcaggagc
    20581 aatagcccaa ccaattctaa aaccaggaag gaatgtcttg gaaaatgtac ctaacaaaat
    20641 cactctttca tcatcgtcga gagaatacat cataggctgc tcctcaccgt caaagcgaag
    20701 ctctcggtaa ggactgtcct ctacaagaat aagatcatat tttttaacga catccagaac
    20761 atatttacgc tgttcgagtg tcattgtcac tcctgaagga ttctgaaaat caggtatggc
    20821 atacacaaac ttcggtttcc tcccttcagc tatcagcttt ttaactgtat cctccaaatc
    20881 atagtctgac ttgatgccta ccggctcagc ctgatatgac cagaaagcct gaagggcacc
    20941 aagataggac ggaagcccac aaacaacagt gtcgcccgga tcaataaaaa tctttgaaac
    21001 aaggtcaata gcctgttgtg aagccgtagt aatcaaaata ttctttgctg aaaccttgca
    21061 gccgttatcg caataccttt tggcaagcag ctcccttaat ttctggttgc cttctgttgg
    21121 accatactga agtgcagatg ccccctcagt ttcaagcact tcaaccatga ttttcttaag
    21181 atcctcaatt gggaaagtgg atgcatttgg gaaaccacca gcaaaagata tgatctcagg
    21241 tctgttagct acgctgagaa gatccctgat tgcagatctt ctcatattct ttgcattgga
    21301 agaaagaaag ttttgaatat ttaccgccat gtcttataat ttttttacaa aagtaaaaaa
    21361 ttgtaataaa aataaaaaaa taaattaaac atttaaatta aaaacctctc cttttgattg
    21421 aattaaaagt gtgtaaagga gtaataaagc aatatttcac ataattacaa atttattaat
    21481 acttttgtaa aaaaacaatg tcgacagtac gcataacaaa agaattccat tttgaaggag
    21541 cgcatgctct caccggttat gatggtaagt gcagacatat ccatggtcac tcatataaat
    21601 tttacgttac tgtcaagggt gaaccggtta acgacacttg caattctaag aatgggatgg
    21661 tacttgactt cagcctgctc aagcaaatcg taaatgaaca gataatcaat ttatttgacc
    21721 attcactcat ccttaataaa gatgcggcgt tatcctcaga gattgaagct gcttaccaaa
    21781 atgtaatatt ggttgatttc caaccaacct gtgaaaacct tgttttacat tttgttgaac
    21841 tgattagatg taaattacct caaaatgtat cattgcaatg cataaagatg tacgagacac
    21901 cttcttctta tgttgagtgg atgacagatg acaattaata actggtttga aaacatgaag
    21961 aaactctatc ttattgatgg acacgcattg atttttcgtt catattacgc atttatcaga
    22021 agaccaatga ttaactcaaa aggtgtcgat acttccattc tctatggttt tacaaaaact
    22081 cttttagacc taataatcaa agaaaagcca acacatattg cagtagcatt tgaccctccg
    22141 gccaaaactt tccgccacga actctatccc gaatacaaag ccaacagagg agaaacaccc
    22201 gaacaagtaa gaggagcact cgagcctcta atatcaatat taaatgcgat ttccatcccg
    22261 gtaataatga aacccggatt cgaagcagac gatgtaatag gaactattgc aaagaaagca
    22321 gaaagagagg gtttcaatgt tttcatggtt acacctgaca aagattttgg gcagcttgta
    22381 tccgacaaca ttgttcaatt taagccagga aaaaatggtg cagatatgga gacaatcggc
    22441 aaagaaaaaa tatgcagtat ttataacata gataagcctg aacaggttat tgacattctg
    22501 actctgcagg gtgatatatc tgataacgtg ccgggtgtga gaggaattgg tgaagtagga
    22561 tcaaaaaaac tggttcaaaa gtatggttca gtagagaaca tttacaataa actggacgaa
    22621 ttgccggaaa aacagcagat tgccttaaag gaagcgaaaa aacacattga attaagcaaa
    22681 agacttatca caatagacac taatgtagat ataccttggg atgaagagtc tcttaaactt
    22741 tccactccaa atttcgaaga acttaaaaga cttttctccg aatacgaatt tagctcctta
    22801 atgagactta tccctaagtt ggaggaggtt tttgaactta caggaaaacc agaggataaa
    22861 tcatgtaaac cacaaaccga gcttaaagct ataaactaca caattgcatc ggcagataag
    22921 gttagaaatg agatttttgc aaagaaagaa cttgccataa agttaataaa agatagattt
    22981 atcatttgtt gtggtgaact aattcagata gttgaagaga caaatatcag acaatacaaa
    23041 gacatccttg aagatggaag tatagtaaaa tgcggttatg accttaagtc cgtaatgaat
    23101 aaactttttg aatatccgat aatattaaaa ggatacatgg cagacataga acttatgcac
    23161 tatcttttaa tgccggagag agctcacaaa gttgaaatta tagttccaac atatctggga
    23221 gtttcactcg atgatcaaag taatgtaatt cagggagatc tttttgatgt taatatatca
    23281 ctaaacgaac attcagaaaa agacttaaaa gagacagccg tgtttttacc gctatatcat
    23341 aaattggcag atgagctgca aaagatgtcc ctcgactctc tatacaacga tatcgagatg
    23401 ccactcataa gtgttttatc gagaatggag accacaggag taaagattga cactgagatt
    23461 ttaaaaaaat tcagtgaaaa attaaattca gaacttactg caatcgaaga agaaatccga
    23521 agaatagctc aagaccctac ccttaacgta tcatctccaa aacaacttgg agtacttctc
    23581 tatgacaaac ttcgacttgg tgccaaaaac ggtaaaaaca ctaaaaaaaa cctttcaact
    23641 gatgaagaga cacttctcga acttgaaaat gaacaccctg taatcccatt aattctcgaa
    23701 ttcagaaata tcaagaaatt aatctctact tatgcagatc ctttacctca aatgatctct
    23761 ccaaaaacag gtaaaattca tacaactttc aatcaagcat tgacatctac aggaaggctc
    23821 agttctgtaa aaccaaatct tcaaaatatc ccaattcgca ctgaaagggg gaaggaaata
    23881 cgcaaggcat ttattccttc ctatcctgaa ggttacatca tgtcctccga ctactcacaa
    23941 attgaattaa gattaatggc tcacatgagc ggtgatgata acttcattga agcgttcaga
    24001 gctggcaaag atattcattc tgcaacagct tcaaaaatat ttgatatacc tgaagatcag
    24061 ctcactaaag aacaaagaag ccgagcaaaa gtcgccaatt ttggaattat ttacggcata
    24121 tctgcattcg gtcttgctca aagactccat atatcaagag gagaagcaaa aaaactgatt
    24181 gatgaatatt ttgcaaaata tcctaaagta caggcattta tcaacaatac tatagaattt
    24241 gctaaaaaaa acggttatgt ggaaactctt tatggcagaa aaagattcat acctgatatt
    24301 aattccaaaa atcccgttgt aagaggttta gctgaaagga acgcagtaaa cgctcctatt
    24361 cagggaagtg cagctgatat cattaagata gcaatgatac gtgtggataa aaggattata
    24421 atggaaggga ttcaaagtag aatgatcctg caagtacatg atgaacttgt tttcgacgtt
    24481 gtcccaagtg aaaaaatgtt gctaacggac cttgtaaaaa aggaaatgga aagtgtagca
    24541 ttactatccg taccacttac tgttgactgc aagacaggca aaaactggct tgaagcccat
    24601 taaacctact cgtatggata caataaacaa atttgatgaa aaagagcttg tcaggatggc
    24661 ggcatcaggt gatcagaatg cttttttaaa actcttcgaa cgatatattg atcagattta
    24721 cacttacatt ttaactttca ttccaaagcc acaggatgca gaagatattt gccaagaaac
    24781 atttcacaaa gcctttaaaa acataatgta ttatcaggat gaatgggctt tttctacctg
    24841 gctatacaaa attgcgcgaa atacggcaat tgatcactac cgcaaaaatt ccttgacccc
    24901 ttatcagtcg atagacgaaa accgagaaga tgcttcagta tttattgacc cttcagatgt
    24961 catgccaagc cctgaagaga acatgatcaa tcaacagaca tttgaaatgt tgatcaaggc
    25021 catcaaagag cttgatgtca aataccgaaa agttgccgaa ctgagattta ttcatgacta
    25081 cgcttatgaa gagatcgcaa gggaattaaa tctgcctcta aatactgtca gaacacagat
    25141 aaaacgagca agaaaattat tggcaaaaaa atggaagaat tgatttacaa atatttcccc
    25201 agactgagtg accagcagag agcaatgtac aaggctcttt atcctcttta tgaagaatgg
    25261 aattccaaaa taaatattat ctcaagaaaa gattttgagc atttctttct gcgccacgta
    25321 cttcactctc tcgccatagc taaattcata tcctttccca atggtgcaag agttttggat
    25381 gtcggtacag gtggaggctt tcccgggatt ccacttgcca taatgtttcc tgaatcatca
    25441 ttttaccttt gcgattccat aagaaaaaaa attacggttg tcggcaatgt ggcttccact
    25501 ttaaatctta acaatgtagt aactgtcaat gcacgcgccg aggaacttga gggccggtac
    25561 gattttgtgg tgtcaagggc agtaactgca ctcaaagatt ttttaccatg gatatggcat
    25621 aaaattgatt ctggattttt taagggtgtt tctcgaggtg ttttgtacct taaaggaggt
    25681 aaccttgaaa acgaaattgc tcttgcctgt tcgaagatga aaatcagtcc ctcacaaata
    25741 gtggaagaga gaattgacca atggtttgta gaaccagatt ttgccgaaaa gaaacttcta
    25801 ttcatcaaac ggtaaacaat tatttgctaa taaaaacatt ttacatatct ttgcacttcc
    25861 ttataaagaa ttataacagt ttaataatat catgaaacgc acattccaac cttcacaacg
    25921 caagcgtcgt aataaacatg gatttcgtga gaggatgtca actccaaacg gacgtcgagt
    25981 tctttcagcc cgtcgtcgtc gtggacgcaa aaaacttaca gtatcgtctg aagaacagtt
    26041 ctagcagata tttactacta taatgagtca agacgcctga ggcgtctttt ttgttatttt
    26101 tgtgtcaata attttaaaat ggagagagat aaaatcatta aggaagctct tgaactgtac
    26161 tcaacttcag acatgaattc aatatccgaa gctgccttga agattagaaa agcttttaac
    26221 ggcaataaag tttattacaa taacaatttc cacatagaac caagtaatat ttgtgtacat
    26281 aggtgtaaat tctgttccta cagaagagac aattcctcac aaccgggagc atggtcaatg
    26341 actcttgaag atgttcgaaa ttactgtcgt gaaaagtatc aaaaaggaat gactgaagtt
    26401 catgttgtgg gaagtgttca tcctgaaaaa agcatcgact attatgtatc catcatctct
    26461 ctactcagga aggaattgcc cgacgaagta acaatcaagg cctatactgc cgttgagatt
    26521 gatgatatgg ccagatattc agggcaaagt gtggagtata tcctaaacaa gctcaaagat
    26581 gcaggactct ctgccatgcc gggtggtggt gcagaaatat tcgattcaga tatccggaaa
    26641 caaatctgcc cagacaagtg caatgccgag agatggatcg aaatccatcg aacagcacat
    26701 aaactcggca taaggagtaa tgccacaatg ttgtttggtc atattgaaaa tcgaaggaat
    26761 agattagatc acatgttgca aatcaaggaa cttcaagagg agactggcgg atttgatgcc
    26821 tttattccac ttctcttcaa atccaccaac aatcagctct cacacctcgg agaaattgga
    26881 tttgtcgaaa ttcttaaaac ctttgccgtt gcaagagttg tactcgataa catacctcat
    26941 atcaaatcct actggccaat gttgggaaaa gatttgagtc agcttacgtt gctatacgga
    27001 gcagatgata tggatggtac aattaataac tccaccaaaa tatacagcat ggccgggtca
    27061 cattcaaatc ccgaaatgag ctgtgacgaa attgaaaata tggccagaga gtgcggattt
    27121 atagcaattg agagagacag cttttataat gaagttaaaa aatagtatta tttttgtgac
    27181 atgaca
//
LOCUS       DAOTQL010000005        25923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_1952_length_25923_cov_5.200209, whole genome shotgun sequence.
ACCESSION   DAOTQL010000005 DAOTQL010000000
VERSION     DAOTQL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1952_length_25923_cov_5.200209"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="P5293_00595"
     CDS             <1..801
                     /locus_tag="P5293_00595"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HRR48464.1"
                     /translation="EVFYCGSKTPSGGSYFRDQFYEIYNNSETVQNVKGLCIAIINPL
                     TATANLPVWAGEDADKYVYALQIWQVPTDQDYPLNPGESIIIAQMADNHQKPTLNPNS
                     PVNLITAEFETLVRTSSIISDNPAINMTMAFWPSPTPQWLTTVFGGAFVIYFPSQPIV
                     PANYVTPVGSTTKCYKIPISEVVDALELVGNVNQMNLKRMPTVLDAGAATVGGTYNGK
                     SVARKVKETVNGRVILYDTNNSTNDFEVMDTPMIRRYGAKAPSWNTWD"
     gene            828..2396
                     /locus_tag="P5293_00600"
     CDS             828..2396
                     /locus_tag="P5293_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48465.1"
                     /translation="MKNFKYIYWAGVLITTLLTTNVVTAQDAAVKRTSPASIERLKAG
                     SLWMDTYNGAGLVLDNLSYYSDLKFAYGYKNGDYKLNSDGERERAITVSSEGGLKFGG
                     GYIWGSFSYVNEAQRNTLFNTTMLDFDRRMPYYTVDKNLSDFLKQSYNLAMKVASKPL
                     WNKVILGAQAEYRTKVGAKQMDPRSETYVYSINVKPSAVVIMGNHRIGINFEYQNLVQ
                     ESTTTNSDTQVNQDVFVLRGLGNGYEATVGGLQSLGMFVYKGNQVGGGLQYSYLMDEY
                     RFLLSGNYIYGMEDVISSPSKPKKEGTVKTQEIEAKLVAIKDGDNLQRLELSYADSKL
                     DGIEYVQQLDNTYEVQRWVVLYKSIRSDYKEQIASFKYDFFRGADKNYKWRAGVLAQY
                     KKNDDIYYIPRSTEMVENLFMGINGKYSFGLGKKSNLLVGANFVYKDNLDGHYNYGGA
                     KPDSYIITDFMTPDFEYMTNSYSMFGGDVSYVTSISKNGRTSLFVKAAVDYYKPVDLD
                     SDRIQSTFTLGFTF"
     gene            2401..4584
                     /locus_tag="P5293_00605"
     CDS             2401..4584
                     /locus_tag="P5293_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48466.1"
                     /translation="MMRKFILTLLLVSLFFSSWAGERGFAIFVDKGTFNVCQTELKAY
                     ESMLRAEGFTTFLLPDDWETPEQVKAQIVKLHDHDNLEGAVFVGQIPVPMIRDAQHFT
                     SAFKMDQKKFPMFNSSVPSDRFYDDFDLKFDFIAKDSLKSLFYYYSLRWDSPQRISCD
                     IYTGRVKPTRSGEEGYRQISDYFNKLVAERTSSNEMNVLVSYTGEGSFSNSLTAWKEE
                     GVTLREQFPQAFHDRNSAKSLLFCMYPYMKDIVIHEMQRNDVDLMFFHEHGTPDRQYL
                     TAIPKANAIDLYPEAAKYLFRERLRKLQKENKDLTEFKKNWMRYYNIDSTWFAGAFDP
                     KQIAEDSLQDIRTGIVLADIPKIKPNPRVVIFDACYNGDFREVDYIAGEYIFSPGKTV
                     VCIGNTVNVLQDKSSSDLLGLLGLGYSIGEWAQQTNILESHIIGDPTFIFKKTIIATK
                     EPFSGRADLRSKDTLYWLNFFNNASHPDLKGLALHKLFELNYRQMSDLLLKTYQTSSY
                     YMLRLQVYHLLQFYMDGNFEKMLMQAVTDPYEFIRRKAVYSMGRIGKDDFIPYIASVY
                     VNDFLDERVEFNAIFCLDLMNLDKMENEVLKQLNEANWLFDKEKKIADFKNVMASRRR
                     MAEYSLDLSNKNLKLKSRQMGVSVLRNNPYHQLVDDYLRILADKDESVELRTDLAEAL
                     GWFTLSYRKSDIVSACRTLAAESGVNPVLRAELLKTANRLEVYMR"
     gene            4595..6850
                     /locus_tag="P5293_00610"
     CDS             4595..6850
                     /locus_tag="P5293_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRR48467.1"
                     /translation="MKIKYIKIAILTGLLFGVTDSFAKSLFIPAEGKFDSRFLIVIDQ
                     KSFRAVKADVLAYKTQLESEGLGAMILAGEWDDPMKLRKEIEVIYKKKPVMEGAVFMG
                     DIPIVRVRNFQHATTAFKMDEEKFPIFESSVTSDRFYDDLDLEFEFLSRDDKFPNLFY
                     YKLKESSPQVVESDFYSARMLPPSDMGFGKYFLLSRYLKKVVAAHKENNPLDKFVVFN
                     GHGYNSDCLTAWQNEQFYMREVAPEAFKVSSGNGFYNYRQDPFMKFKLFEKLQKRGTD
                     LFIFHEHGDFDTQYINGEYPADNIIDFSGDDSTVESIYANSRFNRVGPLNVLSIELRN
                     MYRKYKGDKRENFKKEVIETMGFTDSFFDPEVLEKSRVSDSIFSANFNIRLKDLIAIN
                     PEPKFVIFDACYNGSFHMPGYVAGYYVFGEGGTVVAQGNTVNVLQDKWSLELLGILSE
                     GARVGFWQKEFQLLESHLVGDPTYMFRTEGSKTLNYNLAVKGGDKMIWDKYLHSNNPS
                     LQAMALKQLSKANIHGYSDTLLSVMKSSPFCSVRMEALKRLVDINSSNIAEAITIGLS
                     DPYELIRRNAARYAGYSGIVSIIKPLVNTVLFSNESQRVQYVAQTSLMVMDPDQVVRE
                     IERQLVSNASSVVVENTVKSYKDQYKYQDESLALILDKSAPELKRLTAIRSLRNYNCH
                     RQVDTLLTVLIDSKESLKVRAALAEALGWFKMSYKRPEIIQTMDMLVKQSSLPEDLRS
                     EIEQSIIRLKL"
     gene            complement(6920..9754)
                     /locus_tag="P5293_00615"
     CDS             complement(6920..9754)
                     /locus_tag="P5293_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR48468.1"
                     /translation="MKSRFLTIAFMAISICSFAQKKPLDHSVYDGWKSIGGFSLTKDG
                     GYSMFYINPQEGDSELVSFNVTTGQMDTIHRGNSYKITENGKYAAMTIKPKLAETKAA
                     KRKKLNADQMPKDSLGIYNLQTKELKKYPYLKGMKTARYISDFVAFQTTPPADTAKGK
                     KPAKKEKEEGSDLMIYRFSTGVIDTIKYVTDYDFSKGGDTLFIVQVPNSKDSLYKDCS
                     GLYMYNPYKKSMTAIYNIHKKQTVKLPTVSDNNRTLAFYAKLDTAKVDAKNVSVLVYR
                     DGYEKAKIVADNKMPWLPEKYVISESRSLQMDSTGQRLFFGISPIQREKDTTLMDSEI
                     SKLDIWHYKEPYLQTVQLINLQRDLKRSFVCKVELDGEPAFAQLAKEDYPIVNVPEKW
                     SSQWGYSVSDKNYQLESQWSANPHRDLWIVSVKDGKSKLLLKDVYIQSVSASPEGKFL
                     TWFNLTDRNWYSYEVASDKIRNISKDITTSLTDELHDTPDMAPGYGSCGWMKDDAAFF
                     IKDRYDVWKLDPKGEKSSVNFTDGIGRTEKKSFNIVDLSEMLLPPGTPGVVNDPVKPG
                     ATVYFSVFDEITKDNGYYYKDLKKKKPVMTKWVLEPYTLLYLTKAKKGPVVTYVKQNF
                     TESPNLWITKDNFKTQTKITDINPQQKDYNWGTVELIKWKSKTGIDLEGLLYKPEDFD
                     PSKKYPMIVYFYERNSQYLNLYRAPAPSRSTINVTYFVSNGYLVFIPDITYEIGHPGK
                     SALNCIVPGVEKICENSWADRDNVAIQGQSWGGYQVAYMVTQPQVFKWKAAGAGAPVS
                     NMTSAYGGIRWGTGVVRQFQYEHTQSRIGKNLWDGLDLYLENSPIFFADKIETPLLIM
                     SNDNDEAVPWTQGIEYFTALKRLGKPAWMLQYNKETHNLSDRVNAKDLSIRLSQFFDH
                     YLKGAPMPVWMSKGVPATLKGIDWGLELDE"
     gene            complement(9791..11860)
                     /locus_tag="P5293_00620"
     CDS             complement(9791..11860)
                     /locus_tag="P5293_00620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRR48469.1"
                     /translation="MKNKIIIMLVSTALISGSCKESSKEKNEIPIAKSTITVTDGKFT
                     PEVMQNLGRVSDPQVSPDGTKILYGVSYTSIEQNKSNRELFVMNIDGSDNRQITSTLK
                     SENNARWIDGGKKIAYLYEEQLYVMNSDGSSPKQISNVENGISEFTFSPKGDKILYVS
                     RVKSIEKPVDKYPDLDKATGRIIKDLMYRHWDEFVEAIPHPFIADFDGKSLSNTVDIL
                     EGEPYECPTLPFGDMTQLSWSPDGENIIYSCRKLVGKEYAFSTNTDIYLYNIESKKTE
                     NLTEEMMGYDTDPLYSPDGKSIAWISMRRNGYEADKKRLFVMDLDSRTKRELTIDYPY
                     NVETITWMPDGSGLFFTSCVKALTGVYQVTLADSSIKLLTKGQFDFDRVQVSGDKLYT
                     IYKSMSMPNEIVAINIADSSYNQISFENKAILDQVKMGKIEERWIKTVDNKDMHMWVV
                     YPPDFDSSKVYPALLFCTGGPQGTLSQNWSYRWNFQLMAAQGYIVILPNRRGTTAFGQ
                     EWCEQISGDYPGLNMQDYLSSVKVMKNEKYVGKVGACGASYGGYSIYYLAGIHQGLFS
                     AFLAHAGIFNQEQMYMMTEEMWFPTWDNGGAPWDDNPVAKRHYALSPHKLVKNWNTPI
                     MITHGELDYRVPVEQGMAAFNAAKMLGVPAEMLLFPDENHWILKPQNNILWNRMFFEW
                     FDKWLKN"
     gene            complement(12031..13953)
                     /locus_tag="P5293_00625"
     CDS             complement(12031..13953)
                     /locus_tag="P5293_00625"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRR48470.1"
                     /translation="MQKNKKLFKTIVIALFFICTVISNRVSAQVPVIDSLKRYKDTTR
                     ILPELIINNYKPTPFQPLVRIVSIINKNDIETSTVTNFPDFLRNFSGIDLRNRGSEGV
                     QADLNILGGTFDQTMVLINGINFTDPQTGHYSLDIPISLSQIERIEVLQGPGAINIIT
                     NGNCDSNEVQARITGGSYGFINGTANIRFANGYLPGQTGGSYSQSDGYTENTDFVISN
                     LFSNVTFCLGSGQSLMLMGGYQHKKYGANSFYSVSYPQQFEETRTFLSAIQHMFESKK
                     WQIRSSIYQRRHYDRFELFRYEAEPWYSGHNYHQNDIYGLTAEAAHKWSPESTTIFGA
                     GFRIEHIYSNVLGDSLKSGKEVPFEIDIQYSRSKQRNISTIYVKQTERLGKWLLTAGL
                     TLDHSIGITHLCYGFSSSYSIAPHFEVNAWINNSFRNPTFTDLYYKSPTQTGNMNLRP
                     EEMITGRTEFKYFTNNVLSSLSAFYRRGIRMIDWTRKSGSDQWQASNITNVNSIGLEC
                     SLRFSFDNNYLRSASFLYAWLDVSKKSSGLHSLYATDFLRHKAVFTVEHYLLKDLSAS
                     WILCYQKRAGTYLDKENAETPYKGFILTDLKLSWNIRKFIVSMTAANLFNKDYLYIGN
                     LPQPGRWIKCGVEVRL"
     gene            complement(13940..14533)
                     /locus_tag="P5293_00630"
     CDS             complement(13940..14533)
                     /locus_tag="P5293_00630"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRR48471.1"
                     /translation="MSFIKLAIFASGSGTNAENIIKHFSNHPTCKVSLILSNKKNAYV
                     LERAKRFKIPFVIFDRDQLENSTFVDDIMSSYNINYIILAGFLLKIPDRLLNKYPKKI
                     INIHPALLPKYGGKGMHGMNVHSAVINDGETESGITIHLVNNEYDKGTIIFQAKCNVE
                     KEDTPETLATKIHQLEQQHFPKVIEQYILQDTNYAKK"
     gene            14738..14974
                     /locus_tag="P5293_00635"
     CDS             14738..14974
                     /locus_tag="P5293_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRR48472.1"
                     /translation="MADINEKVKAIIVDKLGVEESDVTPTASFTNDLGADSLDTVELI
                     MEFEKAFGITIPDEAAEKISTVGDAISYIEQHVK"
     gene            14991..16244
                     /gene="fabF"
                     /locus_tag="P5293_00640"
     CDS             14991..16244
                     /gene="fabF"
                     /locus_tag="P5293_00640"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRR48473.1"
                     /translation="MELKRVVVTGIGTINPLGDNIQDYFDNLDKGVSGCGPITRFDTS
                     LFKTKFACEIKNYEPSKYGIDRKEERKLDLYSQFALIAVDQAIKDSHIEAGNFNPERV
                     GVILGSGIGGINTLYSEVEGYVEGGKVPRFSPFIIPKMIPDIAPGLISIKYNFKGPNY
                     ATVSACASSTHAMVDSLNYIRLGKADIIVTGGAEAGISIPSIGGFNSAQAISTRNDDP
                     EHASRPFDKNRDGFVMGEGSGILIFEELEHAKARGAKIYCEVAGAAMTADAYHITAPH
                     PEGEGAITVMKLALEDAGMRPEDVDYINVHGTSTPLGDIAELKAVKVVFGEHAYKLNI
                     SSTKSMHGHLLGATGAVEALACIHAIIDSIIPPTINFEEEDPEIDYKLNLTLNHAQKR
                     EVNVALNNTFGFGGHNSSILFRKFK"
     gene            complement(16529..17704)
                     /locus_tag="P5293_00645"
     CDS             complement(16529..17704)
                     /locus_tag="P5293_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HRR48474.1"
                     /translation="MSNRIKLQPVATLLVTSVIIISCINVDKSVGENLIPSDYELTVA
                     KTSVEIPLQMKMSDSLQSLFPNYLMVGEYNDPDFGRTSCGTAFQMIPIVTENDFGDNP
                     EPVYLRMEVAVDKTVFLNKGEGSIPQNIYLYALKKDIDSTTLLNNSLTRDDYDITPLN
                     IGSTVYTGGDSINIEINLDYARQLLTATKDERDSVSYFVKRFKGFFFSTDPVPSSING
                     GRFNMIDPSSVYMEMKYRHVSATDGIDKDSVLLYYISTSECFYTNIFSHSSKFLETND
                     PQGKILLEGMGGIKPFIDFKDVRQVFQTWANQNNIDLNKVLIAKAELILPYEFPNNYT
                     IMDQYPSSIYLAYKKYSDSYKSTIYYPVDDIYTVGAGTLNRSQYYYSMDISTFFNTY"
     gene            complement(17694..18530)
                     /locus_tag="P5293_00650"
     CDS             complement(17694..18530)
                     /locus_tag="P5293_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRR48475.1"
                     /translation="MSEPKRVLYVSSEIFPYMKESDISIVGRYLPQGAQERGFEIRSF
                     MPKYGIINERRNQLHEVIRLSGMNIIIDDIDRPLIIKVSSIPSARMQVYFIDNEDYFY
                     RKFIYHDSEGRYFDDNDERALFFAKGVLETVKKLRWKPDIVHCQGWISHLVPIFLRKY
                     YHEDPIFTNCKVIVSLYDNEVNNIDFNDKARSKLVAAGIKNKDLDMFSPPNGINLAKL
                     AIQYANGVIMGSKTLPDTISEMIKSNSVPLLPYIEVSQHDTAYIDEYVEFYDQILREN
                     VK"
     gene            18624..19502
                     /locus_tag="P5293_00655"
     CDS             18624..19502
                     /locus_tag="P5293_00655"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRR48476.1"
                     /translation="MKKKEKGIIFRRLVHSYLSSIISISLVLLIVGFLGIIAVNAKAV
                     SDYFKENIRVSVMFDKNASNYDALDFLSKVQGKDYIKRAEFISKERGAEEMKNMLGED
                     FLYVFDFNPIPLSVDIYLKADYLQSDSLKTIVSELSKWPKVREIAYQESLVSLINQNL
                     EKVGIILSVILILLLFISFVLINNTVRLNIYSKRFTIQTMKLVGAKRSFIRKPFIKQA
                     VFQGLISGTISAALLSVLVLLVKRDINQLFLIFDIKMIALVIFGVILAGIFICVISTR
                     IVVNKLLSVSADELYY"
     gene            19516..19767
                     /locus_tag="P5293_00660"
     CDS             19516..19767
                     /locus_tag="P5293_00660"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRR48477.1"
                     /translation="MEKNLKEEQGKFAIPYRNFLLVILGVVLMLTGYALMIGGGSDNP
                     TIFTGDEMFSFRRIVISPLLILLGLVVEVVAIMHVGRSK"
     gene            19764..20555
                     /locus_tag="P5293_00665"
     CDS             19764..20555
                     /locus_tag="P5293_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRR48478.1"
                     /translation="MTALESILLGLLQGLTEFLPVSSSGHLTIGKEIFGISTPDLSFE
                     IVVHAATVLSTIVVFRKDILKLFSGFFRFRMNPETDYILKIFVSMIPVLIVGLFLKDQ
                     VEAIFGSGLLIVGICLIVTALLLTVSSYVKPKEKELSYKNAFIIGLAQAIAVLPGLSR
                     SGSTIATGMMCGIRKDQIARFSFLMVLIPILGEAFLELISGELAASKSGVDTLVLILG
                     FISAFVSGLFACKVMINLVKKTKLVGFAIYCAAVGSICIIYPLIF"
     gene            20558..21310
                     /locus_tag="P5293_00670"
     CDS             20558..21310
                     /locus_tag="P5293_00670"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRR48479.1"
                     /translation="MSSCERYLYYFVSDVHLGLGAYDAPAREERFVKFLRGVPPEIKA
                     LYLLGDIFDYWFEYKYVVPKGFTRVLGALSQLSDSGVELYFFRGNHDMWTFGYLEKEI
                     GLKILKEPSVVEIGQKKFCLAHGDELTGAEPSHLMLKKLFRSKVLQRLLASVHPRWTF
                     PIASKWSTTRKMTNYYLIEFKGKEEPLYKYASEFEKKEKVDYFIFGHIHTPGDAITPS
                     GAGFYILGAWLYGCEYLCYDSRNDKITWMSGG"
     gene            complement(21307..21933)
                     /locus_tag="P5293_00675"
     CDS             complement(21307..21933)
                     /locus_tag="P5293_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRR48480.1"
                     /translation="MQVTKATTIALILLTSMTFASAYSQPKGYLLGYKVEGNDTVYQI
                     RIKDFYFFSRPAYRMKNKDWQEYYRLVYNFKKTYPFALIAKEKITEADSVISSSKFNA
                     REREKYLRSFENELFKEFEKPMRKMTFSQGKLLLRLIDREIGQSSYYIIKSYRGNVNA
                     VFWQGIARIFGSDLKQPYDRFGKDRETEELVRMYQNGTFDYLYYSLFD"
     gene            22064..23146
                     /locus_tag="P5293_00680"
     CDS             22064..23146
                     /locus_tag="P5293_00680"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRR48481.1"
                     /translation="MCKFIFSKLFLLLLIVIPSQLFSQAREALGDSYYTTKADKIHEK
                     IVTIDTHNDSALKLNNSARRNSGSNQVTFSMMEKGGLDAAFFAIYTGQGPRDEESVKD
                     ADFYASDQLLRFRNYVESSDQAGFAYNAHDILYNKGRGQRSVILALENGYPFGKDISQ
                     VERFYNYGVRAVTLCHNYNNDICDASMDTVVEHNGLSPFGYKVVEEMNRLGIIIDVSH
                     ASSKTLSDVLRVSKLPVIASHSGVYAIKNHKRNLTDEEIKAIAAKGGLIQVATGRYFL
                     SDKPKEEVTVKDIADHIDYVKSLVGIRHVGIGTDFDGGGGVVGMENAAKMKNLTVELL
                     KRGYTNRELKMFWGKNILRVLRAHSK"
     gene            23315..23734
                     /gene="rplP"
                     /locus_tag="P5293_00685"
     CDS             23315..23734
                     /gene="rplP"
                     /locus_tag="P5293_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRR48482.1"
                     /translation="MLQPKKTKFRRQQKGRMKGMAQRGNQLSFGSFGIKTTESCWLTG
                     QQIEAARQAVVRYMKREGQIWIRVFPDKPITKKPAEVRMGKGKGAPEGFVCPITPGRM
                     IIEADGVPLDVAKEALRLGAHKLPVNTKFVVRRDYTE"
     gene            23752..23949
                     /gene="rpmC"
                     /locus_tag="P5293_00690"
     CDS             23752..23949
                     /gene="rpmC"
                     /locus_tag="P5293_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRR48483.1"
                     /translation="MKTKEVRELSINDLKERIEAEKANLLRMKMNHAISPIDDLSKMK
                     KSRKDIARMLTILTQKQNIDK"
     gene            23954..24208
                     /gene="rpsQ"
                     /locus_tag="P5293_00695"
     CDS             23954..24208
                     /gene="rpsQ"
                     /locus_tag="P5293_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRR48484.1"
                     /translation="MERNLRKERIGVVVSNKMDKSIVVAVKRKIKHPIYGKFVKKTTK
                     FVAHDENNECSEGDTVRIMETRPLSKTKRWRLVEIIEKVK"
     gene            24212..24577
                     /gene="rplN"
                     /locus_tag="P5293_00700"
     CDS             24212..24577
                     /gene="rplN"
                     /locus_tag="P5293_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRR48485.1"
                     /translation="MIQQETRLLVADNSGAKEVLCIRVLGGTRRRYAEVGDKIVVTVK
                     SAIPGGDAKKGSVSKAVVVRTKKEIRRPDGSYIRFDDNACVLLNNQGEVRGTRIFGPV
                     ARELRENYMKIISLAPEVL"
     gene            24594..24917
                     /gene="rplX"
                     /locus_tag="P5293_00705"
     CDS             24594..24917
                     /gene="rplX"
                     /locus_tag="P5293_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRR48486.1"
                     /translation="MNKKLHIKKGDTVFVNAGDDRGKTGRVLEILKSENRAIVEGINM
                     VSKHTKPNAKYPQGGIIKQEASIHISNLQVVDPVKGGPTRIGRRLNDKGKLVRFAKKS
                     GEEIK"
     gene            24917..25582
                     /gene="rplE"
                     /locus_tag="P5293_00710"
     CDS             24917..25582
                     /gene="rplE"
                     /locus_tag="P5293_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRR48487.1"
                     /translation="MATTKPNTATKSAKAEKKPSVKVEGVQVLVKGYVPNLQKKYKEE
                     IVPKLMKEFGYSSIMQVPALEKITINQGVGSATQDKKLIEVAQQELTSITGQKAIMTY
                     SKKDISNFKLRRGMPIGVKVTLRKAKMYEFLERLISVSLPRIRDFKGISDKFDGRGNY
                     TLGIKEQIIFPEVDIDKITKIFGMEITFVTTAGKDEEAYALLREFGLPFKNIKKEVIF
                     KTE"
     gene            25591..25860
                     /gene="rpsN"
                     /locus_tag="P5293_00715"
     CDS             25591..25860
                     /gene="rpsN"
                     /locus_tag="P5293_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRR48488.1"
                     /translation="MAKESMKAREIKRAKLCAKYAEKRKALKAIGDYAALDKLPKNSS
                     PVRLHNRCSITGRPKGYMRTFGISRIQFREMASKGLIPGVRKASW"
BASE COUNT         7885 a         4380 c         5255 g         8403 t
ORIGIN      
        1 gaagtttttt attgtggttc aaagacccct tcaggaggtt catatttcag ggatcagttt
       61 tatgagattt ataacaacag tgaaacagtc caaaatgtta aaggcctatg tatagccatt
      121 atcaatcctt tgactgccac tgcaaatctt cctgtatggg caggagaaga tgctgataaa
      181 tatgtatatg cacttcagat ctggcaagta cctacagatc aggattatcc tctgaacccg
      241 ggcgaatcta taataattgc acaaatggcg gataatcatc aaaaaccgac attgaatcca
      301 aatagtcctg tgaatctaat tacggctgaa tttgagacgc ttgtcaggac aagttcgatt
      361 ataagtgata atccggcaat taacatgact atggcatttt ggccgtcccc tactcctcaa
      421 tggctgacaa cagtgtttgg aggggctttc gtaatctatt tcccatcaca gccaatagtg
      481 cctgcaaatt atgtaacgcc tgtaggttcg acaacaaaat gttacaagat tccaatttcg
      541 gaagttgttg atgctctgga attagtgggt aatgttaacc agatgaatct taagaggatg
      601 ccaacagtgc tcgatgcagg tgcagctaca gtcggaggaa cttataatgg taaaagtgtt
      661 gccagaaaag ttaaggagac tgtaaatgga agagttatcc tttatgatac aaataacagt
      721 accaacgatt ttgaagtgat ggatactcct atgatacggc gatatggtgc aaaagctcct
      781 tcatggaata cttgggatta gtgtttaaca aaagcaacga tatgacaatg aaaaatttta
      841 aatacatata ttgggcggga gtcttgataa ctacacttct tactactaat gtggtaacag
      901 cccaggatgc tgcggttaaa cgtacatctc cggcatccat tgaacgactt aaagccggat
      961 cgctttggat ggatacttat aatggagcag gtctggtttt ggataatctt tcatattaca
     1021 gtgaccttaa gtttgcttat ggttataaaa acggggacta taaacttaac agtgatggag
     1081 agagagaaag agcaataaca gtctcttcgg aaggtggtct caaatttgga ggtggttata
     1141 tttggggtag cttctcatat gtcaatgaag cgcagaggaa cacactgttt aatactacaa
     1201 tgctcgattt tgacagaaga atgccatatt atacagttga caaaaatttg agtgactttc
     1261 ttaaacagtc ctataatctg gccatgaaag tcgccagtaa accattgtgg aataaagtta
     1321 tacttggtgc acaagcagaa tacagaacaa aagttggtgc taaacaaatg gatccacgtt
     1381 cggaaacata tgtttattct ataaacgtca aaccttctgc agtagtgatt atgggcaatc
     1441 atcgaatcgg aattaatttc gaatatcaaa atcttgttca ggaatcaaca actactaaca
     1501 gtgacactca agtcaatcag gacgtgtttg ttctcagagg gttgggcaat ggttatgaag
     1561 ctactgtcgg aggtcttcaa tctctcggca tgtttgtgta caagggcaac caggttggtg
     1621 gaggtctcca gtactcatat ttgatggatg agtacaggtt tttactgtct ggaaattata
     1681 tttatgggat ggaggatgta attagttccc catcaaaacc taaaaaggaa ggtacagtta
     1741 aaactcagga gatagaagca aaacttgttg caatcaagga tggtgataat cttcagaggc
     1801 ttgaactatc ttatgccgac agcaagttgg atggtatcga atatgtccag caactggata
     1861 atacttatga agttcagcgc tgggtggttc tttacaaaag catacgttct gattataagg
     1921 agcaaatagc ctctttcaag tatgatttct tcagaggtgc tgataaaaac tacaaatgga
     1981 gagctggtgt tctggcacaa tataagaaga acgatgatat ttactatatc ccaagatcta
     2041 cagaaatggt agaaaatctc tttatgggca tcaacggcaa atatagtttt ggccttggga
     2101 aaaagtctaa tcttctggta ggtgctaact ttgtttataa ggacaatttg gatggtcatt
     2161 ataattatgg aggtgcaaaa ccagactcat atatcattac agatttcatg acacccgatt
     2221 ttgaatacat gaccaacagc tattcaatgt ttggtggtga tgttagttat gtaacatcta
     2281 taagtaaaaa tggacgtaca agtctgtttg tcaaggcagc cgttgattat tacaaacctg
     2341 tcgatttgga tagtgacagg attcagtcaa cattcacact tggctttaca ttctaacagg
     2401 atgatgagaa aatttattct taccttattg ctggtctctc tcttcttctc ctcttgggct
     2461 ggagagagag gctttgcaat ttttgtggac aaagggactt tcaacgtgtg tcaaactgaa
     2521 ttgaaggcgt acgaatcaat gttaagagct gagggtttta ctacatttct tttacctgac
     2581 gattgggaaa ctcctgaaca ggtgaaagct cagatagtaa aacttcatga ccatgacaat
     2641 cttgaaggag ctgtttttgt tggtcagatt cctgttccta tgatcagaga tgcgcagcac
     2701 tttacctctg ctttcaaaat ggatcaaaaa aaatttccga tgtttaattc ctctgtgcct
     2761 tctgatcgtt tctatgatga ttttgacctg aaatttgact ttattgcaaa agattctctc
     2821 aagagtcttt tctattatta ttcgctaaga tgggattcgc ctcaaagaat ttcatgtgat
     2881 atttatactg gaagggtgaa acctaccaga agtggtgagg agggatacag acaaataagt
     2941 gattatttca ataaacttgt ggctgaaaga acttcttcca atgaaatgaa tgttcttgtc
     3001 tcttataccg gtgagggatc tttttccaac agtcttactg cttggaaaga ggaaggagta
     3061 acattaagag aacaatttcc tcaggcattt catgacagga actctgcaaa atctttgttg
     3121 ttctgcatgt atccatatat gaaggacatt gtgattcatg aaatgcaaag aaatgatgtg
     3181 gatttgatgt tttttcatga gcacgggact cctgacagac aatatttaac agctatacct
     3241 aaggcaaatg ctattgatct atatccagaa gcagctaaat atctttttag agaaagattg
     3301 agaaagctgc aaaaggaaaa taaagacctt actgaattca aaaagaactg gatgagatat
     3361 tataatatcg attctacctg gtttgcagga gcgtttgatc cgaagcagat agctgaggat
     3421 tctttgcaag atatacgtac tggcattgta ctggcggata ttccgaaaat taagcccaat
     3481 ccaagagtag taatatttga tgcctgctat aatggagatt ttcgtgaggt tgactatatt
     3541 gcaggagaat atattttttc tcccggtaag acagttgtat gtattggtaa tactgtaaat
     3601 gttttgcagg acaagtcttc aagcgatctg ttaggccttt tggggcttgg ttacagtatt
     3661 ggagaatggg cgcagcagac aaatattctc gaatctcata ttattggaga tccaacattt
     3721 attttcaaaa aaacgatcat tgctactaaa gaaccttttt ctggaagagc tgacttgagg
     3781 tcaaaagaca ctctgtattg gcttaacttt ttcaataatg ccagtcatcc agatcttaaa
     3841 ggacttgcac ttcataaact ttttgaacta aattatagac aaatgtctga tttgttgttg
     3901 aaaacatatc aaacatcttc atattacatg cttaggttgc aggtttatca tttgttgcaa
     3961 ttctatatgg atggcaattt tgaaaagatg ttgatgcagg ctgtaaccga tccatatgag
     4021 tttataagaa gaaaagctgt ttattctatg ggaagaatag ggaaggatga tttcatccca
     4081 tatatagcct cggtgtatgt taatgatttt ctcgacgaaa gggttgagtt taatgcaatt
     4141 ttctgtcttg atctgatgaa tttggacaaa atggaaaacg aggttttgaa gcaattaaac
     4201 gaagcgaact ggttatttga taaggaaaaa aagatagcag attttaaaaa tgtaatggct
     4261 tcaagacgca gaatggcaga atacagtctt gatctgtcaa acaagaatct taaactcaag
     4321 tctcgccaga tgggggtcag cgtgttgagg aacaatccgt atcatcagct ggttgacgat
     4381 tatttgagga tacttgccga caaggatgaa tctgtggaat tgaggactga tcttgcagag
     4441 gctctgggct ggtttacact ttcatataga aaaagtgaca tcgtatctgc atgcagaacc
     4501 ctggcagcag aaagtggtgt caatcctgtt ctgagagcag aactgctcaa aactgcaaat
     4561 aggttggaag tttatatgcg ttagaattga aaagatgaaa attaaatata taaaaatagc
     4621 gatacttacc ggtctgcttt ttggtgtgac tgattcgttt gcaaaaagtc tgtttattcc
     4681 agcagaggga aaatttgata gtagattttt aatagttatt gatcaaaaga gttttagagc
     4741 tgttaaggct gacgttttgg cctataaaac tcaacttgaa agtgaaggtc ttggtgcaat
     4801 gattctggct ggagagtggg atgatccaat gaaactcagg aaagagatag aggtgatcta
     4861 caagaagaaa ccagtgatgg aaggggccgt gtttatgggg gatataccta ttgtcagagt
     4921 caggaatttt caacatgcca caactgcttt taagatggat gaagaaaaat tcccaatatt
     4981 tgaatcttca gtcacttctg acaggtttta tgatgacctt gatcttgaat ttgagttttt
     5041 aagcagggat gataaatttc cgaatctttt ctattacaag ctaaaggaga gctctcccca
     5101 ggttgtggaa agcgattttt attctgcccg tatgttgccg ccttccgata tgggatttgg
     5161 taagtatttt cttctttcga gatatcttaa aaaggttgtt gccgctcaca aagagaataa
     5221 tccgcttgac aagtttgtcg ttttcaatgg tcacggatat aattccgatt gtcttaccgc
     5281 atggcagaac gaacaattct acatgcgcga ggttgctccg gaggctttca aggtttcttc
     5341 aggcaatggc ttttataact atcgtcagga tccatttatg aaattcaaac tttttgaaaa
     5401 attacagaag cgtgggacag atctttttat atttcatgag catggggatt ttgacactca
     5461 atatataaac ggagaatatc cggctgacaa tattattgat ttttcaggtg acgatagcac
     5521 agttgaatcc atttatgcaa attccagatt taacagagtc ggtcccttaa atgttctatc
     5581 tatagaatta agaaacatgt atcgcaaata taaaggcgac aaacgggaga attttaagaa
     5641 agaggttatt gaaaccatgg gttttacaga ctcctttttt gatcctgagg tcttggagaa
     5701 aagtagagtg agtgattcaa ttttttctgc caattttaat attcgtctta aggatctcat
     5761 agccattaat ccagaaccaa aatttgtaat ttttgacgct tgttataatg gttcatttca
     5821 tatgcctgga tatgtcgccg gatattatgt ttttggagaa ggcggtacag tagtggcgca
     5881 gggcaacact gtcaatgtct tgcaagataa gtggtcgctg gaattacttg gaattctcag
     5941 tgaaggagca agggtaggct tttggcaaaa agaattccaa ttacttgaat ctcatttagt
     6001 tggtgatcct acttatatgt ttagaacaga agggagtaaa actttgaact ataatttggc
     6061 agttaagggt ggcgacaaaa tgatttggga caaatatctg cattccaaca acccctcatt
     6121 acaggccatg gcacttaaac aactttcaaa agctaacata catggttatt cagatacttt
     6181 gctttctgtt atgaagagca gtcctttttg cagtgtaaga atggaggctt taaagagact
     6241 tgttgatatt aacagtagta atattgctga agctattaca atcggactaa gcgatcctta
     6301 tgaacttatc agaagaaatg ctgcacgata cgcaggatat tccggaatag tatctataat
     6361 taagccactt gtaaatacgg ttttattttc gaatgagagt caaagagttc aatatgtagc
     6421 gcaaacttca cttatggtta tggatcccga ccaggtggtc agagagattg aaagacagct
     6481 tgtttcaaac gctagctcag ttgttgtaga gaatactgtt aagagctata aagatcagta
     6541 caagtatcag gatgaatcac ttgctttgat tttagataaa tcagctcctg aattaaagag
     6601 actgactgca ataagaagtt tgagaaatta taattgtcac aggcaggtag atacactctt
     6661 aactgtgttg atagattcaa aggagagtct aaaagtaaga gctgctttag ccgaagcatt
     6721 aggctggttc aaaatgagtt ataaaagacc agagattatt caaacaatgg atatgcttgt
     6781 gaaacaatcc tcgctaccag aagatttaag gtcagaaata gagcaaagta ttataagatt
     6841 gaagttatga tagtaaaaaa gccgactcga aatcatgtga tataagccgg cttttttatt
     6901 ttaataatat atttgatatc tattcatcaa gttctaaccc ccaatctatc cctttcaggg
     6961 ttgcaggaac acctttgctc atccatacag gcataggtgc tcccttgagg tagtgatcaa
     7021 agaactgaga gagtcttatt gagagatcct ttgcattgac acggtcagaa agattgtggg
     7081 tctccttgtt gtactgaagc atccaggcag gttttccgag tctctttagt gcagtaaaat
     7141 attcaatgcc ttgtgtccat gggactgcct catcgttgtc gtttgacatt atcagcagag
     7201 gtgtttctat cttatcggcg aagaaaattg gagagttctc gaggtataga tccagaccat
     7261 cccataaatt tttaccaatt cggctttggg tatgctcgta ttggaactgc cgtacaacac
     7321 ctgtacccca gcgaattcct ccatatgcac tagtcatgtt ggaaacaggt gcgcctgcac
     7381 ctgcggcttt ccatttgaat acttgaggct gtgtaaccat atatgcaact tgatatcccc
     7441 cccaactttg accctgaatg gcaacattat ctctatcagc ccatgaattt tcacagattt
     7501 tttcaacacc cggaacaata cagttcaagg cacttttacc tggatgaccg atttcgtaag
     7561 ttatatccgg aataaatacg aggtatccgt tgctgacaaa atatgtgaca ttgattgtag
     7621 agcgacttgg agcaggggcg cggtataaat tgagatattg tgaatttctt tcataaaaat
     7681 agacaatcat tggatatttt tttgaaggat caaaatcttc cggtttgtat agcaggcctt
     7741 caagatcaat tcctgttttt gacttccatt ttatcagttc aactgttccc cagttataat
     7801 ccttctgctg agggtttata tctgtaattt ttgtctgtgt tttgaagtta tcttttgtga
     7861 tccaaagatt aggagattct gtaaagttct gtttaacata tgtaacaaca ggtccttttt
     7921 ttgcttttgt tagatatagc aatgtgtaag gctcaagaac ccatttggtc attacaggtt
     7981 ttttcttttt aagatcttta taataatagc cattatcttt agttatttca tcaaatacgg
     8041 aaaaatatac cgtagcacca ggtttaacag gatcatttac aaccccagga gtaccaggag
     8101 ggagaagcat ttcgcttaaa tctacaatgt tgaaactttt cttttctgtt cttccaatcc
     8161 cgtcggtaaa attgacagaa gatttttcac cttttggatc gagtttccac acatcatacc
     8221 ggtctttgat aaagaaggca gcatcatctt tcatccatcc gcatgaacca tatccgggag
     8281 ccatatccgg tgtgtcatga agctcgtcag tcaatgatgt agtaatatct ttagagatat
     8341 tcctaatctt gtcggaagca acttcatagg aataccagtt tctgtctgta agattgaacc
     8401 atgtaaggaa ttttccttcg ggagaggcag atactgattg aatgtaaaca tcttttaata
     8461 acaatttact tttcccgtct tttacagaga ctatccagag gtccctgtga ggatttgccg
     8521 accactgtga ctccagctga taatttttgt ctgaaacaga atatccccac tggctggacc
     8581 atttttcagg aacattgacg attggataat cttcttttgc taattgagca aaggctggtt
     8641 caccatctaa ttcgacttta caaacaaatg acctctttaa gtctctttgt aaattaataa
     8701 gttgtactgt ctgaagataa ggttctttgt aatgccagat atcaagttta gatatttctg
     8761 aatccatgag agtagtatcc ttttcacgct ggattggaga tattccgaaa aataggcgtt
     8821 gtcctgttga atccatttga agagagcgtg attcagagat cacgtatttt tccggaagcc
     8881 atggcatttt gttgtctgcg acaattttgg ctttttcgta tccatcacgg tataccagca
     8941 ctgatacatt ttttgcatcg acttttgctg tatcaagttt tgcataaaaa gcaagtgtgc
     9001 gattattatc gcttacagta ggaagtttga ctgtttgttt tttgtgtatg ttgtaaatag
     9061 ccgtcatact ctttttgtag ggattataca tatataatcc tgaacagtcc ttgtagagtg
     9121 agtccttgga attaggcact tggacaataa agagagtgtc gcctcccttg gaaaaatcat
     9181 agtctgttac atactttatg gtatctatga cgccagtgga aaatctgtaa atcattaaat
     9241 cactgccttc ttctttttcc tttttagctg gctttttccc ttttgcagta tctgcaggag
     9301 gtgttgtctg gaatgcaaca aaatcgctaa tgtatctggc tgtcttcata cctttgagat
     9361 atggatactt ttttaactcc tttgtttgca gattgtaaat tcccaatgaa tctttaggca
     9421 tttgatctgc attaagtttt ttacgtttag cagcctttgt ctcagcaagt tttggcttta
     9481 tggtcatggc ggcatacttg ccattttcgg taattttata agagttgcct ctatgaatag
     9541 tgtccatctg acctgttgtc acattaaaag aaacaagttc agaatctccc tcttgggggt
     9601 tgatataaaa cattgaatat ccgccgtctt ttgtaaggct gaatcctcca atagatttcc
     9661 atccgtcata aacagaatgg tcaagcggtt ttttctgcgc aaatgagcag attgatattg
     9721 ccataaaggc aatcgtaagg aatctggatt tcattaatat tgttttaatt aaattgtgtt
     9781 agtatttatt ttaattttta agccacttgt cgaaccattc gaaaaacatt ctgttccaca
     9841 aaatgttatt ttgaggtttg agaatccagt gattttcgtc tgggaagagt agcatttcgg
     9901 caggtacacc cagcatcttt gcggcattaa aagctgccat gccctgttct acaggaactc
     9961 tgtaatccag ttccccatga gttatcatga taggagtatt ccaattttta acaagtttgt
    10021 gaggtgaaag tgcataatgt cgtttggcaa caggattgtc atcccatgga gcaccaccat
    10081 tatcccatgt agggaaccac atttcttcgg tcatcatata catctgttcc tggttgaaaa
    10141 tgcctgcatg tgcaagaaat gctgagaaga gaccttggtg tatgccggca agatagtaaa
    10201 ttgaatatcc gccataactg gctccacagg cgccaacttt gcctacatat ttctcgtttt
    10261 tcatcacttt gacagaggag agatagtcct gcatattcaa tccgggataa tctcctgaaa
    10321 tttgttcaca ccattcttgt ccaaaagcag ttgtgcctct tctgttaggc aaaataacta
    10381 tatagccttg ggcagccatt agttgaaaat tccatctgta cgaccaattt tggctgagag
    10441 ttccttgtgg gccaccggta cagaagagca gtgcagggta gacctttgat gaatcaaaat
    10501 ctggcggata gacaacccac atatgcatat ctttattgtc taccgttttt atccatcttt
    10561 cctctatttt tcccattttc acctggtcaa gaattgcctt gttttcaaag cttatttgat
    10621 tgtaagaact atcagcaata tttattgcta ctatttcatt gggcattgac atacttttgt
    10681 aaatagtata aagtttatct cccgagactt gaacccgatc gaaatcaaat tgtccttttg
    10741 tgagcaactt tattgaactg tcagcaagtg tcacttgata cactccagtt aatgctttta
    10801 cacaagaggt aaagaaaagt cctgagccat ccggcatcca ggtaatagtt tcgacattgt
    10861 aggggtaatc gattgttagc tctctttttg ttcttgaatc aagatccatt acaaaaagcc
    10921 tttttttgtc agcttcatag ccattacgtc tcatgcttat ccatgcaatc gattttccat
    10981 caggagaata tagaggatct gtatcatatc ccatcatttc ctcagtaaga ttctcggttt
    11041 ttttcgattc aatattgtat aaataaatat ccgtatttgt cgagaatgcg tattcctttc
    11101 ccactaactt ccggcaggag tagatgatgt tttctccgtc tggactccag cttagctgag
    11161 tcatgtcacc aaatggcaga gtcgggcatt catatggttc tccttccaat atgtctacgg
    11221 tatttgaaag tgatttgcca tcaaaatcag caataaaagg gtggggtatt gcttcaacga
    11281 actcgtccca gtggcgatac atcaaatcct ttattattct tcccgttgcc ttgtcaaggt
    11341 ccggatattt gtcaacaggt ttttcaattg acttaaccct acttacatac aatattttat
    11401 ctcctttagg actaaaggta aattctgaga ttccattttc gacattactg atctgttttg
    11461 gagaagagcc atcactattc attacataca attgttcttc gtatagatag gcaatctttt
    11521 tgcctccatc tatccaacga gcattatttt cacttttgag agttgatgta atttgtctgt
    11581 tgtcactccc gtcaatgttc attacaaaaa gctctctatt gctcttgttt tgctcaatac
    11641 ttgtatatga gactccgtat aagatttttg ttccgtcagg agaaacctgt ggatcagaca
    11701 ctcttccgag attttgcata acctctggtg taaatttgcc gtcagtgaca gtgattgtgc
    11761 tctttgctat tggaatttca tttttctctt tggaagactc cttacacgag ccggaaatta
    11821 aagctgtgga aactaacatg ataatgattt tgtttttcat gaggttaaga ggttgattat
    11881 tcattattct attatatatt ttgatatttt ttttactatt tggtttaata tatagtaaaa
    11941 ttaaatcaat tatactctta ttccaaaaaa gaggaaatat tacttaagga atatatgttc
    12001 cctgtgattt tccaactcaa ataggtttca tcagagtctg acctcaaccc cgcactttat
    12061 ccatctgccg ggttggggca gatttcctat gtaaagataa tctttattga atagatttgc
    12121 ggcagtcatt gagacgataa atttcctaat attccatgaa agtttaagat ctgttaaaat
    12181 aaagcctttg taaggagttt cagcattctc tttgtcaagg taggtcccgg cacgtttctg
    12241 gtagcaaagt atccaacttg ccgacaaatc ttttaacaaa tagtgttcaa cagtgaatac
    12301 agctttatga cgaaggaaat ctgtggcata gagtgaatgc aatcctgaac ttttctttga
    12361 aacatctaac catgcataaa gaaaactggc acttcttaga taattgttgt caaaagaaaa
    12421 tctcagcgaa cattcaagac ctattgaatt aacatttgta atattacttg cttgccactg
    12481 atcggatccg cttttccgtg tccagtcaat catccttata cctcttctgt aaaatgccga
    12541 taaagaggat aaaacattgt tggtgaaata tttaaactcg gttctacctg taatcatttc
    12601 ttctgggcgc agattcatat tgcctgtttg tgtcgggctt ttgtaatata aatctgtgaa
    12661 tgtgggattc ctgaatgaat tatttatcca tgcattaact tcaaagtgag gtgctataga
    12721 ataagagctt gaaaatccgt aacataagtg agttatgcct attgaatgat caagagtcaa
    12781 tcctgcagtt aacagccatt tacctagtct ttctgtttgt tttacatata ttgtagaaat
    12841 attgcgttgt tttgagcgtg agtactgaat atctatctca aatggcactt ctttcccact
    12901 tttcagggaa tctcctaaaa cattgctgta tatgtgttca attctaaagc cggctccaaa
    12961 tattgttgtg ctttcaggac tccatttgtg tgctgcttct gcagttaagc cataaatgtc
    13021 attttggtga tagttgtgtc cagaatacca aggttctgct tcgtagcgaa acaattcaaa
    13081 tctgtcatag tgtcttcttt ggtatatcga actcctgatt tgccatttct tactttcgaa
    13141 catgtgttga attgcagaga gaaatgtcct cgtttcttca aactgttgag ggtaggatac
    13201 ggaatagaaa ctattggcac catatttttt atgctggtaa ccacccatta acataagtga
    13261 ctgtccagat cccaagcaaa aagttacgtt tgagaataaa tttgagatca caaaatcggt
    13321 gttttcagta taaccgtctg attggctgta acttccgcct gtttgtcccg gcaaatagcc
    13381 gtttgcgaat cttatgttcg cagtaccatt aataaaccca taacttccac ctgtaattct
    13441 tgcctgtact tcattactgt cacagttacc gttagtaata atattgatag cacccggacc
    13501 ttgcagcacc tcaattctct caatctgtga tagcgatatg ggaatatcta gagaatagtg
    13561 accagtctga gggtctgtga agttaatgcc attgatcaaa accattgttt gatcaaaagt
    13621 gcctccaagg atattgagat ccgcctgaac accttcactg cctctgtttc tgagatcaat
    13681 gccggagaaa tttctgagaa aatccggaaa attggttaca gtacttgtct caatgtcatt
    13741 tttgtttata attgatacaa ttcttacaag gggttgaaac ggagtaggtt tatagttatt
    13801 gataatcagt tctggtaaaa ttctggtggt atctttatat cttttcagac tgtcgattac
    13861 gggcacttgt gcagaaacgc gatttgagat aactgtgcat atgaaaaata gagctataac
    13921 aatagttttg aatagctttt tatttttttg cataattagt gtcttgcaga atgtattgtt
    13981 caatcacttt tggaaaatgt tgctgttcaa gttgatgtat tttggttgca agtgtttcag
    14041 gtgtatcttc tttttccaca ttacattttg cctgaaagat gatagtccct ttgtcgtatt
    14101 cattatttac aagatgaata gtaatacctg attctgtttc tccatcattg ataacagcag
    14161 aatggacgtt catcccatgc attccttttc ctccatattt aggaagtaag gcagggtgaa
    14221 tatttattat tttttttgga tatttattta aaagtctgtc cggaatttta agtaaaaatc
    14281 ctgcaagtat tatgtaattt atattgtaac tcgacattat atcatctaca aatgtgctgt
    14341 tttctagttg atcccggtca aatattacaa agggtatttt gaatcgtttt gctctctcca
    14401 gcacataggc attttttttg ttactcagta taaggctgac cttgcatgta ggatgattgc
    14461 tgaaatgttt aattatattt tccgcatttg ttcctgaccc tgaggcaaaa atagctaatt
    14521 ttataaaact cattattaac attttaagct gcaaaggtaa cattttttaa ttagataaat
    14581 gatttataag tgattgttta attatacttt ttcctctata tatatgtata ccaaaaaact
    14641 attgtttcac tttgttaaaa tttcattttt ccattttttt tgtttttctt tgcaacctgg
    14701 tttagaaaat aataattact aactaatttt tttaattatg gcagatatta atgaaaaggt
    14761 taaagccatc attgttgaca agttgggcgt agaggagtca gatgtaacac caacagcaag
    14821 ttttaccaat gatcttggcg cagattcact tgacacagtg gaacttatta tggaatttga
    14881 aaaagcattc ggtatcacaa ttcctgacga agctgctgag aagatctcaa cagtaggaga
    14941 tgctatctct tacattgaac agcacgttaa ataaattccc aatcaagcat atggaattaa
    15001 aaagagttgt agttaccggt atagggacaa taaatccttt aggcgacaat attcaggatt
    15061 attttgacaa tctggataaa ggcgtcagtg gctgtggtcc tattacccgt tttgatacat
    15121 ctctgttcaa aacaaaattt gcttgtgaaa tcaaaaatta tgagccgagt aaatatggca
    15181 ttgatcgaaa ggaagagcgg aagcttgacc tctattcaca atttgctctt attgctgtag
    15241 atcaggcgat aaaagatagt catattgaag caggaaattt taatcctgag agagtagggg
    15301 taatactcgg ttcagggata ggtggtatta atacattgta cagtgaagtt gagggctatg
    15361 tcgaaggcgg taaagtgcct agatttagcc cttttattat tccaaaaatg attcctgaca
    15421 tagcgcctgg tcttatttcc attaagtata actttaaagg accaaattat gctactgtgt
    15481 ctgcatgtgc ttcttctact catgcaatgg tagattcttt aaattatatc agactcggga
    15541 aagcagacat tattgttact ggtggtgctg aagcaggtat ttctattcca agtataggag
    15601 gttttaactc tgcacaggca atatccacca gaaatgatga tccggaacat gcttcaaggc
    15661 ctttcgacaa aaaccgtgat ggttttgtaa tgggcgaagg atccggaatt ttgatttttg
    15721 aagaattgga gcatgcaaaa gccagggggg ctaaaattta ttgtgaagtt gccggtgcag
    15781 ccatgactgc agatgcttac catattactg cacctcatcc tgagggagaa ggcgcaataa
    15841 ccgtaatgaa acttgctttg gaagatgcag gcatgagacc tgaagatgtg gattatatta
    15901 atgtacacgg gacttccaca cctcttggcg atattgctga attaaaagct gtaaaggtcg
    15961 tttttgggga gcatgcatat aaacttaaca taagttctac aaaatccatg catgggcatc
    16021 ttcttggagc cacaggtgct gtggaagctc ttgcgtgtat tcatgctatc atagacagta
    16081 ttattccgcc tacaattaat tttgaggaag aagatcctga gatagattac aagcttaact
    16141 tgactctgaa ccacgcacaa aaacgtgaag taaatgtggc actgaataat acctttgggt
    16201 ttggaggaca taactcctca attttattca gaaagtttaa gtaataattt tgttgaggaa
    16261 tataaaaaca caataggctg tctaaaagta ggcagcctgt tttttatatg aaaatttcaa
    16321 taaataaaat caaataggaa aaaaggtcaa ggtaaaacag tatatgtaat ctcgattcga
    16381 ggtttacgtg tgcttttagg accatttaaa gtcgctttgc aatatatttg attatccaca
    16441 tagtaagata cggcaccggt atatgaattg gttagttgag taattgggtt aatccatatt
    16501 tgccatttct cattatctat ggtattattt agtacgtgtt gaaaaatgtg ctaatatcca
    16561 ttgagtagta atactgggat ctgttaagag tacctgcacc tacggtatat atatcatcta
    16621 caggataata tattgttgac ttatatgaat cagaatattt tttgtaagca agatatatcg
    16681 atgaaggata ttgatccatt atagtataat tatttgggaa ctcatatgga agaattaatt
    16741 cagctttggc tataagaacc ttattaagat ctatgttgtt ttgatttgcc caagtctgaa
    16801 atacttgtcg aacatcttta aagtcgataa aaggttttat tcctcccatt ccttcaagca
    16861 aaattttgcc ttggggatcg tttgtttcaa gaaatttgga gctgtgagag aagatgtttg
    16921 tatagaaaca ctcgcttgtg gagatataat agagaagcac gctgtctttg tcaatgccgt
    16981 ctgtcgccga tacatgtcta tatttcatct ccatgtatac tgatgacggg tcaatcatgt
    17041 taaaccggcc accattgatc gatgaaggaa caggatctgt cgagaagaaa aaccctttaa
    17101 accttttaac gaaataactt acagagtcac gttcgtcttt agtcgcagtt agtagttgac
    17161 gtgcgtaatc gagattgatt tctatgttta tggaatcacc tccagtatag actgtgcttc
    17221 caatgtttag gggagttata tcataatcgt cccttgtaag agaattgttt aaaagcgttg
    17281 tagaatcaat gtcttttttc agagcataaa gatagatgtt ctgagggatg cttccttctc
    17341 ctttattaag gaatacagtc ttgtcaactg caacttccat tctcagatat actggttccg
    17401 gattgtcgcc aaaatcgttt tcagtcacaa taggaatcat ctgaaaagca gttccacaag
    17461 aagtgcgtcc aaaatctgga tcattgtatt ctccaaccat gaggtaattt gggaaaagag
    17521 attggagaga atccgacatc ttcatttgca gaggaatttc tacagatgtt ttggcaactg
    17581 taagctcata gtccgatggt ataaggtttt cccctacact tttgtcaaca tttatgcaag
    17641 atataattat tactgaagtc actaacagtg tcgcgacagg ttggagctta atcctatttg
    17701 acattttccc ttaaaatttg atcataaaat tcaacatact catctatgta tgctgtgtcg
    17761 tgttgtgaaa cttcaatata aggcaataaa ggcacagaat tgctttttat catttcagaa
    17821 atagtatcag gcaatgtttt acttcccata atgacaccgt tcgcatactg aatggctaat
    17881 ttagcaagat ttatgccatt gggtggactg aacatgtcca gatctttgtt ctttataccg
    17941 gcagctacta gcttagaacg tgctttatcg ttaaaatcga tattattgac ttcattatcg
    18001 tacaatgaaa caataacctt gcaatttgtg aaaataggat cttcatgata atatttcctg
    18061 agaaaaatgg gcactaagtg tgatatccat ccctggcagt gaacaatatc aggtttccac
    18121 cttagcttct tgacagtttc aagtactccc ttggcaaaaa atagagccct ttcatcatta
    18181 tcatcaaagt atctgccttc agaatcgtga taaataaact ttctgtaaaa ataatcttca
    18241 ttatctatga agtaaacctg catccttgct gatggtatag aggatacttt gataatgagg
    18301 ggcctgtcaa tatcgtcgat aataatgttc atgcctgaga ggcgtatgac ttcatgaagc
    18361 tgatttctcc tttcatttat gataccgtac ttaggcatga atgagcgtat ttcgaatcct
    18421 ctttcctgtg ctccctgagg taaatatctt cccacaattg aaatatcaga ttctttcata
    18481 taagggaaaa tctcagaact cacataaagt actctttttg gttcgctcat cgtactatct
    18541 tgtttttaaa ggatatttct tacaaaggta ataaatttat ttaatttaaa ggaattatat
    18601 atctttggaa gcttttaaac actatgaaga agaaggaaaa aggcataatt ttcagaagac
    18661 tggtacattc ttatctttcg tcaattataa gtatctctct tgttttactt attgtgggct
    18721 ttcttggtat aattgcggtt aatgcaaaag ctgtttctga ctattttaaa gaaaacatca
    18781 gagtgtcagt aatgtttgat aagaatgcaa gcaattatga tgctcttgac tttttaagta
    18841 aagttcaggg taaagattat atcaaaagag ctgagtttat atcaaaagaa cgtggagcag
    18901 aggaaatgaa aaacatgctt ggcgaagatt ttctctacgt ttttgacttt aatcctattc
    18961 ctctttctgt ggatatatat ttaaaagcag attatttaca gagtgacagt ctcaagacaa
    19021 tagtttcgga actttccaaa tggcctaaag ttagggaaat tgcctaccag gagtcacttg
    19081 tgagtctaat aaatcagaat cttgagaaag taggcattat tttgagtgta attcttatat
    19141 tattgctttt tatttctttt gttttgatca ataatacggt gcgtttgaat atttattcaa
    19201 agcggttcac tattcaaacc atgaaacttg ttggagcaaa gcggtcattt ataaggaaac
    19261 catttatcaa gcaggcggtg tttcaggggc tgatttccgg aacaatatca gctgctttac
    19321 tttcagtact tgttttatta gtcaagagag atataaatca gctgtttctt atatttgata
    19381 ttaagatgat agctttagtt atttttggtg tgattcttgc cggcattttt atatgtgtga
    19441 taagtacaag gatagtagta aacaagttgc tttcggtttc agctgatgag ttatattatt
    19501 aatagacaat tttatatgga gaaaaattta aaagaagaac agggcaagtt tgcaattcct
    19561 tacaggaatt ttcttcttgt aattttggga gttgtattga tgttgacagg atacgctctt
    19621 atgatcggag gtgggtcaga taacccaacc atttttacgg gagatgaaat gttcagtttc
    19681 aggcggattg tcatatctcc tcttttgatt cttttgggac tggttgttga ggttgttgca
    19741 attatgcatg ttggcagaag caaatgacag cgcttgagtc aatattgctt ggtctccttc
    19801 aggggttgac tgaatttctg cctgtgagca gtagcggtca cttgacaatt ggtaaggaaa
    19861 ttttcggtat aagtacacct gatctaagct ttgaaatcgt ggttcatgct gctacggttc
    19921 tcagtacaat tgtggtcttt agaaaggata ttcttaaatt gttttccggt tttttccgtt
    19981 tcagaatgaa tcctgaaaca gactatattt tgaaaatttt tgtgtcaatg atccctgttc
    20041 taatcgtggg tcttttttta aaggatcagg ttgaagcgat atttggttca gggcttctta
    20101 ttgtcggaat atgccttatt gttacagcac tattgttgac tgtttcaagc tatgtcaagc
    20161 caaaagagaa agaattatca tataaaaatg catttattat tggtttggca caggctattg
    20221 ccgtacttcc cggtctttcc cgttcgggtt cgactattgc aaccgggatg atgtgtggaa
    20281 ttaggaaaga tcagatagca agattttctt ttcttatggt tcttattcca attcttgggg
    20341 aggcttttct tgagcttata tcaggtgaac ttgctgcttc aaaaagcgga gtggatactc
    20401 ttgtattgat acttggattt atttctgcat ttgtttccgg cttgtttgca tgtaaagtaa
    20461 tgattaatct tgttaagaaa actaaactgg ttggttttgc aatttattgt gcggcagttg
    20521 gatctatttg tattatttat ccattaattt tctaaatatg tcatcctgtg aaagatattt
    20581 atattatttt gtatctgatg tgcatttagg tctaggtgct tatgatgcac cggcaaggga
    20641 ggagaggttt gttaaatttc taagaggagt tcctcctgaa ataaaggcgt tatatttatt
    20701 aggagacatt tttgactatt ggtttgaata caaatatgtt gttcccaagg gttttacaag
    20761 agtgctgggg gcgttgtcac aattgtctga tagtggtgtg gaactctatt tttttagagg
    20821 gaatcatgac atgtggactt ttggatatct tgagaaggag ataggtctta aaatacttaa
    20881 agagccatct gtagttgaaa ttggacaaaa gaagttttgt cttgctcatg gggatgagct
    20941 tacaggagca gaaccctcac atttaatgct gaaaaagctt ttcaggtcaa aagtgcttca
    21001 gagattactg gcaagtgtac atcccaggtg gactttccca atagcttcta agtggtcaac
    21061 tacaagaaaa atgacaaact actatcttat tgaatttaaa ggaaaagaag agccgcttta
    21121 taaatacgcc tctgaattcg aaaaaaagga aaaagtcgat tattttattt tcggtcatat
    21181 acatactccc ggtgatgcga taaccccatc aggtgctggt ttttatattc taggcgcttg
    21241 gttgtatggt tgtgaatatc tttgctatga ttcaagaaat gacaagataa cctggatgtc
    21301 tggcggctag tcaaagagcg agtaatacaa atagtcgaat gtgccattct gatacattct
    21361 gacaagctct tctgtttctc tgtctttgcc aaatctgtcg tatggttgtt tgagatcact
    21421 accaaatatt cttgctatac cttgccagaa gacagcatta acatttcctc tatagctttt
    21481 gattatgtaa tatgagcttt gtccaatctc tctatctatt aatcgtaaca acaatttgcc
    21541 ctgagaaaat gtcatttttc tcataggttt ttcaaactct ttaaatagct cattctcgaa
    21601 agatctcaaa tacttttctc tttctcttgc attaaatttt gaggaagata ttacactgtc
    21661 ggcctcagtt attttctctt ttgcaatcag agcaaaaggg taagtcttct taaagttgta
    21721 aaccaatcta taatactctt gccagtcttt atttttcatt ctgtaagctg gacgtgagaa
    21781 aaaatagaag tctttgattc taatttgata tacagtatca ttgccttcga ctttgtatcc
    21841 caaaagatat ccttttggtt gagaatatgc ggaggcaaaa gtcatgctcg taagtaaaat
    21901 gagagcaatc gttgttgctt tggtcacttg catcacttgc atcaaatttt gaaaacttac
    21961 ctatacaaat ataatgcctg tcaataatta ggccaaatta tttaatttat tacatttgtt
    22021 agaacaaatc ctatattaaa tccttaatta ctcagtcaac tttatgtgta aatttatatt
    22081 ttccaaactg tttttattgc tactgattgt aataccgtcg caactttttt ctcaagcacg
    22141 ggaagcactg ggtgatagct attatacgac aaaagctgac aaaattcatg agaagattgt
    22201 tacaattgat actcacaatg attctgctct caaactgaat aattccgcca gacggaactc
    22261 aggtagtaat caggttacat tttccatgat ggagaaagga ggtcttgatg ctgctttttt
    22321 tgcaatatat actgggcagg gcccaagaga tgaggaatca gtcaaagatg cagattttta
    22381 tgcatcagac caattgctga gatttaggaa ttatgtcgaa tcgagtgacc aggctggttt
    22441 tgcctataat gctcatgata ttttatacaa taagggtaga gggcaaagat ctgtaattct
    22501 tgcacttgaa aatggatatc cttttgggaa ggacatttca caagttgaac gtttttataa
    22561 ttatggtgta agggcagtta ctttgtgtca taactacaat aatgatattt gtgatgcctc
    22621 aatggacaca gttgttgaac acaatggatt atctcctttt ggttacaaag ttgttgagga
    22681 gatgaacaga cttggtataa taattgatgt ttcacatgca tcttccaaaa ctttatcaga
    22741 tgttttacgt gtcagtaaac tcccagtgat agcaagtcat tcaggagttt atgctataaa
    22801 aaaccataaa aggaacctta ccgatgagga aattaaggca attgctgcca aaggaggatt
    22861 gattcaagtt gccaccggca gatattttct atctgacaag ccgaaagaag aggttacagt
    22921 caaagacata gcggaccata ttgattatgt caagtcactt gtaggtatca ggcatgttgg
    22981 cattggaaca gattttgacg gtggcggtgg agttgttgga atggaaaatg cagccaaaat
    23041 gaagaacctt accgtagaac ttttaaaaag aggatatacc aatagagagc ttaaaatgtt
    23101 ttgggggaag aatattttaa gagtcttaag agcacattcc aagtaatttt ttatcagtta
    23161 tttttgcacg tttgattttt ttggtatatt tgcaacccgc cagaaatgtg attgatttac
    23221 atatatagct gtgggacagg gtaatgacct tcattttttt aagaccaaag cattaaaaac
    23281 atatttactg ttatagttaa actgtaaaaa agcaatgtta caaccaaaaa aaaccaaatt
    23341 cagaaggcag cagaagggcc gtatgaaggg aatggcacaa aggggcaatc agctttcatt
    23401 tggatcattc ggcattaaaa ccactgagtc ctgctggctt accggccaac agatagaagc
    23461 tgctcgtcag gctgtagtaa gatacatgaa acgtgaaggt cagatttgga tccgcgtttt
    23521 tcctgataaa ccaataacaa aaaaacctgc tgaggtgcgt atgggtaaag gtaaaggagc
    23581 tcccgagggg tttgtttgtc caattactcc gggtcgtatg attatagagg ctgatggagt
    23641 tccacttgac gttgccaaag aagctcttcg tcttggggca cataaacttc ctgtgaatac
    23701 gaaatttgta gtacgtagag attatactga ataattaaat taggagaaaa gatgaagaca
    23761 aaagaggtaa gagaattatc gattaatgat ctcaaggaga ggattgaagc tgaaaaggct
    23821 aatcttcttc gcatgaagat gaatcatgct atctctccta tagatgattt atcaaagatg
    23881 aaaaaatcaa gaaaggacat tgccagaatg cttaccattc tgacccaaaa acaaaatata
    23941 gacaaataga gctatggaaa gaaatcttag aaaagagaga ataggcgtag tggtaagtaa
    24001 caaaatggac aagtctattg tagtagctgt aaaaagaaaa atcaaacacc cgatttacgg
    24061 caaatttgtg aagaaaacca ctaagtttgt agctcatgac gagaataatg agtgcagtga
    24121 aggtgataca gtacgtatta tggaaactcg tcctttgagc aagacaaaac gttggagatt
    24181 agtagaaata attgaaaaag ttaaataagc catgatacag caagaaacga gattattggt
    24241 ggcagataac agcggagcaa aggaagtact ttgcatccga gttttgggtg gtacccgtcg
    24301 ccgttatgct gaagttggag acaaaattgt tgttacagta aaaagtgcta tcccgggcgg
    24361 agatgcaaaa aaaggttctg tttcaaaagc agttgtggta cgtacaaaaa aggaaattcg
    24421 tcgtcctgat ggttcatata ttcgttttga tgataacgca tgtgtgttgc tcaacaatca
    24481 gggagaagtt cgcggtaccc gtatctttgg tccagtggcc agagaattgc gtgagaatta
    24541 catgaagatc atttctttag ctcctgaggt tttgtaaaat aaatatagaa gacatgaaca
    24601 aaaaattaca tattaaaaaa ggtgatacag tttttgtgaa tgcaggagac gaccgtggta
    24661 aaaccggtag agtacttgaa attctcaaaa gtgaaaatcg tgccatagtt gagggtatca
    24721 atatggtgag caaacataca aaacctaacg caaaatatcc tcagggaggt attattaaac
    24781 aggaagctag tattcatatt tccaacctgc aggttgtaga tcccgtaaaa ggcggtccta
    24841 caagaattgg tcgtcgtttg aatgacaagg gcaaattagt tcgtttcgcg aaaaagtcgg
    24901 gggaggagat taaataatgg caactacaaa accaaatact gcaactaaaa gtgctaaagc
    24961 tgaaaaaaaa ccttctgtca aagttgaagg tgttcaggtt ttagtaaaag gatatgtgcc
    25021 aaatctacag aagaagtaca aagaagagat tgtgccaaag ttaatgaaag aatttgggta
    25081 ttcttcaata atgcaagtgc ctgctcttga aaaaattact ataaatcaag gagttggttc
    25141 tgcaacacag gataaaaaat tgattgaagt agctcaacag gaactgactt ctattacagg
    25201 gcagaaggcc atcatgacat attcaaaaaa ggatatctct aatttcaagc ttcgaagagg
    25261 aatgcctatc ggagttaagg taacgcttcg taaggccaag atgtatgagt ttcttgaaag
    25321 gcttatttct gtatcacttc ctcgtattag agattttaag ggaataagtg ataaatttga
    25381 tggaagagga aactacacat tgggtataaa ggaacaaatc atattccctg aggttgatat
    25441 tgacaagatt acaaagatct tcggaatgga aataactttt gtaacgacag ccggcaaaga
    25501 tgaagaagca tatgctcttc ttcgtgaatt tggtttgccg tttaagaata ttaaaaaaga
    25561 agtaatcttt aaaacagaat aaagtagata atggcaaagg aatcaatgaa agcacgcgaa
    25621 attaaacgtg caaagttatg tgccaaatat gctgaaaaac gcaaagcact caaagcaata
    25681 ggcgattatg ctgcgcttga taaacttcca aaaaattcaa gcccggtacg tttgcacaat
    25741 cgctgctcta taacaggtcg tccaaaaggg tatatgcgta ctttcggcat cagtcgtatt
    25801 cagtttcgtg agatggccag taagggtctt atcccaggag tacgaaaggc aagttggtaa
    25861 taattataaa aattttaaat aacattggat atcgggcatc ctttttttgg atgtcggtac
    25921 ttt
//
LOCUS       DAOTQL010000006        24961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2072_length_24961_cov_5.046977, whole genome shotgun sequence.
ACCESSION   DAOTQL010000006 DAOTQL010000000
VERSION     DAOTQL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_24961_cov_5.046977"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="P5293_00720"
     CDS             complement(<1..1327)
                     /locus_tag="P5293_00720"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRR48489.1"
                     /translation="MPAERDTLIKKSDSVRVDSLSNDSLNAPKGSIKMPLFSEAKDSV
                     VEDFSNNRKIIYYYGDVKVKYGNLELTADYMEYNIDSQTVFARGNIDTSGNVIGLPKM
                     KEGKSEYVMENVHYNFKSQKAKIKNMITQESEGYIHGDVIKKMPDNSININKGKYTTC
                     ELDHPHFYLKLTRARVINEPRNTVFGPAYLVVEDVPTPFALPFGFVPKQAKRASGLLI
                     PSVTEEAARGFGLTGLGYYFVFGDHLDFTVTTDLFSLGSYNIQMESRYKVRYKFDGGF
                     SLNYSRNKVGEPGTEGYYVTKDFAVKWTHSQDSKAHPGTSFRASVNFSSPSNNRYNSR
                     EIQQTLQNQISSSVSYSRTFAGTPFSFSANLLHSQNSLDSSYAFTFPNFTFNVNRIYP
                     FKRKEAAGKEKFYEKIQFAYNATLDNKINFKSKEFGEADFMQKFKAGVKH"
     gene            1501..2703
                     /locus_tag="P5293_00725"
     CDS             1501..2703
                     /locus_tag="P5293_00725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR48490.1"
                     /translation="MPHRLLLSFFCLLVLSTPLLATDPVKIRKVVIDAGHGGQDPGAI
                     SPNRKVLEKDITLSVALKFGKLIEDKNPDIQVIYTRKTDVFVPLNNRSEIANKNQADL
                     FISIHVNSVKARSACGSETYVMGESKSSSNLEVTMMENSVIAFEGDSKSVYEGFDPNN
                     PESYIIFSLLQNAHLEKSLQMAEYVQEQLAEGPIKVNRGIKQGGLLVLWKTSMPAILV
                     ELGFISNPSDYLMLCGEENHMKYAKALYKAFEKYRKAYEAGNRTSISNPTATATATAT
                     ATATATTTTTSSAAAIPSIQDSSRDTATKNTASTKTTTSDKTSSKSSTSSISYPYRIQ
                     ILAVTKKLPDNAPDLKGEKNVNCIKVGNYFKYSIGEFKTREEAMKELPNVKIKFPQAF
                     ITKVENEN"
     gene            2693..3610
                     /locus_tag="P5293_00730"
     CDS             2693..3610
                     /locus_tag="P5293_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRR48491.1"
                     /translation="MKIKLNKEQKIGLFAIIIAVSLYFTINYLKGKDIFGSTNTYYTV
                     FDNVEGLSATSPIYIKGYKVGTILEIKYLEQQEKFLVTMKVDAKYHIPQNSAAQLYSS
                     SILGGKSIRINIVNGGKIIGERDTLRSEIEPGIIDNIAAQIIPLKDQATELIANLNKT
                     FVSVNEVLNDQAKEQLSVSIANLDKTLKSIKAIASNIEGKNPEIASIITNLDTLSFSL
                     NKSAAALDKGLADITEVTSSLKKADIEGTITSLKNVLIELNNPNGSIGKLIKTDSLHT
                     SIDSLVRDIDVLVKNIQKDPKKYIKISVF"
     gene            3781..5193
                     /gene="dnaA"
                     /locus_tag="P5293_00735"
     CDS             3781..5193
                     /gene="dnaA"
                     /locus_tag="P5293_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRR48492.1"
                     /translation="MSEAPHIKVWNNCLQIVKDNIPLQSFKTWFEPIKAVKLTDNVLT
                     IAVPTHFFVEFLEEYYIDLISKALKRELGKDAKLEYNVRVVSNENPVQLPHQSSAEIT
                     NKALPFPNQEKQIANPFVIPGLQQLKIDPQLNPHFCFDNFIEGECNRLARCAGLTIAS
                     NPGASAFNPLFLYGGSGLGKTHLAQAIGIKIKELFPDKIVLYVSANRFQTQFMDAVNI
                     KNKLTDFLHFYQMIDVLIIDDVQEFAGKSGTQNAFFHIFSHLHNLGRQLILTSDKPPV
                     DLQDLEQRLLSRFKWGLLAELRQPDYQTRMAIIKAKCYNDGIMLEDEVVSYLASRVTT
                     NIREIEGTLNSLLAQAIFNKRTIDMELAESLTEKIVSRNRNEITIEQIQKTVCNYFKI
                     NPELFLSSSRKRELVQARQIAMFLSRNLTNNSLSTIGSLTGGRDHATVLHAYNTVCDL
                     IDTDKSFRKYVTDIEKKLKP"
     gene            5199..6632
                     /locus_tag="P5293_00740"
     CDS             5199..6632
                     /locus_tag="P5293_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRR48493.1"
                     /translation="MDSNKIISYFSEICQIPRESGHEEKIIEFLANFARTHALEYKID
                     DAGNVLISKNATPGRENSPTVVLQSHSDMVCEKNSNSNHDFSKDPIKFIEEDGWLIAK
                     DTTLGADCGIGMAAQMAVLTDDTLEHGKIEALFTTDEETGLTGAKAIKPGFITGKFLI
                     NLDSEDEGEIFIGCAGGIDTTAVFNIPHEKPAKSSEFIKAGFYGATGGHSGDDINKNR
                     ANANQLLVRFLLPLFDKNIINICKIDGGNKRNAISREAFAIVAFNPSYREAILEHFKK
                     VENEVKEEYNISDPNLFGKLEETEPQAWCIDSNTASRILNSLNACPHGVLAMSQDIPD
                     FVETSTNLASVKTSNDTVTVSTSQRSSLNSARYYAASRIESLFKLAGAQVTHESEYPG
                     WKPNVNSKLLEISRQAYKRLFGYEPVVRAIHAGLECGLFLDKYPDLDMVSFGPTLKGV
                     HAPGEKLEIASVEKFWKLLVEILKNLK"
     gene            6640..7290
                     /gene="rpe"
                     /locus_tag="P5293_00745"
     CDS             6640..7290
                     /gene="rpe"
                     /locus_tag="P5293_00745"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRR48494.1"
                     /translation="MGVLIAPSMLSADFGNLSKEVEMVNNSEADLIHLDIMDGLFVPN
                     ISYGFPVVKSIAKNAKKPLDAHLMIIDPDRYVERFRDLGVQYLSVHYEACIHLHRSLQ
                     KIKDLGMKAGVALNPHTPSHLITEIACETDFILIMSVNPGFGGQGFIENSYDKVRRIK
                     ELLSDRASTCLIEVDGGVSKENAASLVEAGADILVAGNSIFGSENPVKAISDLKHI"
     gene            complement(7268..8020)
                     /locus_tag="P5293_00750"
     CDS             complement(7268..8020)
                     /locus_tag="P5293_00750"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRR48495.1"
                     /translation="MSDNIIWFETLDSTNSEAYRRMEGAEDFTVFAARFQTNGRGQKG
                     NTWESAVGENLTFSILVKPACLKAEDQFLLSEAAALGVVSYLTGKGLEAKIKWPNDIY
                     VLDNKICGILIEHYLGSDKLSASILGIGVNINQKKFASNAPNPTSLFLETGKIYKLED
                     ELVLLSSFIKRFYNMINQNAYEQMAGTINNEYLSKLYRLREFHFYEDTGTNEIFEAQI
                     VGIDQNACLLLERRGGEVTSYAFKEIKYVLNH"
     gene            8103..8486
                     /gene="rsfS"
                     /locus_tag="P5293_00755"
     CDS             8103..8486
                     /gene="rsfS"
                     /locus_tag="P5293_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRR48496.1"
                     /translation="MPTTKIKKQSGSDALEVQCIADAMLDKKGKGVCSLDLKKIGTAI
                     TDYFIVCNADSPTQVNAIADHVEDMMYTQCNRKVTRMQGKENSFWVILDYTNIVVHIF
                     QTEYRLFYRLEELWADAPKINYEEK"
     gene            8494..10467
                     /gene="ftsH"
                     /locus_tag="P5293_00760"
     CDS             8494..10467
                     /gene="ftsH"
                     /locus_tag="P5293_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRR48497.1"
                     /translation="MDIKNNNNNINRNNKIKQPKFNAIWIYVILLGGIFYMWATYDGG
                     NPLKTEWVDVKEKMAPSGDIEKISFITNMNRAEVYIKKDSLAKYKNKFGGKIPKFAPQ
                     FYFVVSSNFNAEEQIDMLRQQLPENSRFTFENEERTDYFGKIIEWLLFPIILVALWLF
                     MFRRMNKGIGGGAQGGGIFSVGKSQAKLFDKENNIKVTFKDVAGLEEAKIEVMEIVDF
                     LKNPKKYTSLGGKIPKGALLIGPPGTGKTLLAKAVAGEANVPFFSISGSDFVEMFVGV
                     GASRVRDLFRQAKEKAPCIVFIDEIDAVGRARSKNAGFSSNDERENTLNQLLTEMDGF
                     GSNSGVIILAATNRADILDKALMRAGRFDRQIHVDLPELKERMEIFKVHLKNLKLIEG
                     FNVEFLAKQTPGFSGADIANVCNEAALIAARKNKSAIDKQDFLDAIDRIVAGLEKRSK
                     IISPEEKKTIAYHEAGHATVSWMLPNANPLLKVTIIPRGKALGAAWYLPEERQITTTE
                     QMMDELSATLGGRAAEEIINQKISSGALNDLEKVTKQAYAMVCYLGMSKAIGNVSFYD
                     SSESSGFTLSKPYSEKTAELIDKEVKKFIDESYETAKKILKENMEGFVKLAEQLLEKE
                     VIFSEDLEKIFGKRAGSQPPSREDDTTSTSSEK"
     gene            10464..11336
                     /locus_tag="P5293_00765"
     CDS             10464..11336
                     /locus_tag="P5293_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HRR48498.1"
                     /translation="MTGRFKGTFVRSITGIVFITIMVGSMFLSPVAFGAVMTIAIVFM
                     MAEYMMIAVDVKLSLTKILTIIAGIMLYVLFFLYKAYGIDAKWLYLLTIPFASIFVSL
                     LCYRDNSKSDGTATDPEVYNRHPYLLTSLIYIALPFSMTSLILFDSDLNYRPLTLLSL
                     FIILWTSDVGAYTFGMLFGQKNGHKLCPSISPKKSWEGFFGGIFCGLVAGIVVWILSL
                     TGFSLTHNIIIALIISLAGAAGDLVESQFKRNFGAKDSGKLLPGHGGLLDRFDAALIA
                     FPLAITYIKLFSLI"
     gene            11343..12053
                     /locus_tag="P5293_00770"
     CDS             11343..12053
                     /locus_tag="P5293_00770"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRR48499.1"
                     /translation="MGIHKEGRGIITKSFFLLAAIIIALFFIIGLVTAFIISAIVALI
                     IFFIIIRFFRSPKRTPITEEGAVIAPADGRIVIVKEVYEDEFLKCNCIQVSVFMSLLD
                     VHVNWFPVSGQIKYYKYHPGRYIIAMHPKSSDKNERTSIVIEENGFPVLFRQIAGFLA
                     RRIVCYAVEGREVKKGSEMGFIKFGSRVDLFLPMDSRIQVTKGQKVKGCQTVIARLPE
                     LTEAQKFLYNNNKNSAFY"
     gene            complement(12050..12943)
                     /gene="pyrF"
                     /locus_tag="P5293_00775"
     CDS             complement(12050..12943)
                     /gene="pyrF"
                     /locus_tag="P5293_00775"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRR48500.1"
                     /translation="MTYLELYNNIIKKRSFLCVGLDPDSAQIPDQLKNLEFPLFEFNK
                     RIIDATAMYAVAYKPNTAFYEAEGAVGWRQLEMTVDYLRKKDQSLFVIADAKRGDIGN
                     SSEKYAKAFFEKMDFDAVTLSPYMGKDSIMPFLQYSGKWAAVLALTSNVSADDFETLP
                     LLGHIQMLDDLSAMYDSEGFFYSTTETVGFPLFKSVIEKSMRWGNINNIMYVVGATRP
                     QKLAEIRIFCPNHFFLVPGVGSQGGTIAEVAKYGMNKNCGLLVNISRDIIYAGKGKDF
                     ADAAASKAAEAAKEMSLFLKD"
     gene            complement(12976..14070)
                     /gene="prfA"
                     /locus_tag="P5293_00780"
     CDS             complement(12976..14070)
                     /gene="prfA"
                     /locus_tag="P5293_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRR48501.1"
                     /translation="MSDNNLLSKLSGLREKYENISAQLSNPDVMSDMKRYVSLNKEYK
                     ELEPVVEAGDRYGKLLRDYESAREMLLTEKDEELREMVKEELSAIEEKIPVVEEEIKL
                     LLIPADPQDSKNAILEIRAGAGGDEASIFAGDLFRMYSKFAESKGWKLEVTSKSEGTS
                     GGFKELICTISGQGVYGVLKYESGVHRVQRVPQTETQGRVHTSAASVAVLPEADEFDI
                     DLNINDIRKDTFCSSGPGGQSVNTTYSAIRLTHIPTGIVVQCQDEKSQLKNYDKALAE
                     LRTRLYNLEYEKYLNEIAAKRKTMVSTGDRSAKIRTYNYPQSRVTDHRINYTMYNLPS
                     FMDGNIQELIDQLQIAENAERLKASGENNI"
     gene            14181..14741
                     /locus_tag="P5293_00785"
     CDS             14181..14741
                     /locus_tag="P5293_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRR48502.1"
                     /translation="MSNNGENKKNNSASDFEQMYISNYHKIRSFCNNYLHDNDTAKSI
                     AQDVFISVWNNRDNLNFSDELLPYLFILAKNMCLNVLKRGKVKQNYNDYEKRHNRDSL
                     NYASLKEQTINSLYRKEVEKLINDSLLKMPESVRSTFCISRFKNLKYEEIAVIQKISV
                     KTVEYRIMYALRVLRKILKDYLPIFL"
     gene            14783..15817
                     /locus_tag="P5293_00790"
     CDS             14783..15817
                     /locus_tag="P5293_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRR48503.1"
                     /translation="MKKSLLLKYIRRETTSPEEDDVLKWINKSPDNERYFIGLNNLWI
                     SQNISQDKASKEEVDEIKALTTNKKTSRFKYKKVTYYVAAAIILISAGLNFFLIKENS
                     LLQHKINEPIQLAELPSAYKHELYTEKGVKAKIILPDSSYVWLNSGSKIIFPDRFERN
                     IREIEFSGEAFFDVHKDSLRPLVIKTNKNFKVEVRGTKFNLKTYDNDKEARTTLYSGS
                     LKVISEVKGKNIHSKEVITQLKPLETCIIRGYEKPVLLRPNNVNDHQAWKNGEIVFDS
                     TPMEEVVKILERWHGINFEVKDKYVYNYNITAKFKSESIVQIMEMIKYCAPLDYSIDS
                     TNVFLFKRKL"
     gene            15885..19433
                     /locus_tag="P5293_00795"
     CDS             15885..19433
                     /locus_tag="P5293_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR48504.1"
                     /translation="MMNSKTVPKVLVCFISFVAMFTIFLNNAYAQKININFKDTPIKE
                     VIKEISQQTGYNFVYSNALTAINDKVSISYSADKAPVEKLLDILFNERGISYKIDKKQ
                     IILAPKNIVPVNNNNQPTSQTKNQDQERFTLKGKVLDESKLPLVGVTVLNLSTKKGTY
                     TDNNGNYSIEVKEGDKVQFILIGMATKEITVTKGLTVSNIEMMSDQIRLQEVVVTGYQ
                     TISKERVTGAVKTIRTDMLEKPSTNIAQRLIGTAAGVQATLDVDGNPTFLIRGLTSLN
                     ATREPLVVIDGFPTEKGFNSINPNDVESVTFLKDAAAASIWGAKAANGVIVITSKKAK
                     GEIPLRIEFNAFYRVAPKFDLDYVRPMAKSSDIIDYELLSFNKWGARAYNVGNFLDGY
                     YRTYTPTQELIWQNYIGAISESKMNEEINKYRNLDNSKQIKKHLLDNPSTQQYNLTIM
                     SGTQKMSNYFSLLYEQNNSNYKNTNNNREVISYRNVAKITKWLDFEFGATLAYQVNNN
                     NGINLSNIQSWAPHQMLLDENGQYTNIPNGMNYLPAMTTVPFDKFPYKFTYNPIEEIN
                     NRRITTKSLDTRITGALFIKIFDGLSLESRAQYEYNNTFNKSYYNENTYTVRSAVNAA
                     ATWDKTVGTVTLNLPKGGFLDQNRSEVKALTVRNQLNYRKTIGKHDLTALAGIELTDR
                     VSQTYGYPRTYGYDDNKLTVGTFPNGPGGSSIKIIKNWIDNNQTFSYTNSFSYSTRRY
                     FSSYANASYTYDSKYSLSGSIRSDASNFITDDPKYRYAPFWSIGASWNVNKENFMANV
                     NFIDRLTARLTFGYNGNVDVTTSFQPLITPSSSVNIYTQENTTAISSMGNPTLRWEKT
                     GTWNFGIDYSILKGNLYGSIDLYRKYTRDLIATISIPSVNGSTSSKMNNAEMSNKGIE
                     IEIGTTQKIYGDNIWWRGTLNFAYNKNEITKLFVSQYASYDLYGGAYVEGYNANELWC
                     FKYAGIKNEGSAEHPNWQPQVQGPGDVTYNFTGWTPGDARNYMLHMGTTVAPYTLGFV
                     NDFKIFDFNLSFILTGKFGHVFKRLSFNYPVWWNGAVIPNQKYSEVVNGDPMKIVPLP
                     LNNDEPRFYFWNRFYPNLSYLVEDASHIRMQEINLSYNLPQNLLQKMGLSRAQLYLQC
                     NDVFTIYANKFGEDPEYPIGSQNPQPKFIFGLKFDL"
     gene            19455..21065
                     /locus_tag="P5293_00800"
     CDS             19455..21065
                     /locus_tag="P5293_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR48505.1"
                     /translation="MKYKINIKILNILLLLFILPSCSKIDDFLDERPSKSTSKPLETV
                     EELDGILGAYSSFYTESSATLFAGHDDFGLIKEIHDSYAGIYGGTSMAYHFYPAVWDI
                     KNLEISGDKGWAESWSKIFKANMVLNNIDNVKGSESLKADLKAEAHFIRAYEMYMLAN
                     VFCLPYTNFNKSNKDELGLPLKQSTSFEESVERKTIEETYKFIEADLQEALKITTPLI
                     RQSDGVLRAWRGNKTGVKAFAARYYLYQGDYEQALHYANLALGEYNTLVDYNLNSKNG
                     GMTWGLPKAYTINQGTPQQQTVTLKYPYGHDNQSDPTDRMAWKEHLYMRFMYNGSWWF
                     IPSRELLALYDQNYDRRYEYNYVQGYSYDRGCVKPAYSYPGYIRFFKDNLPSGPTVAE
                     MYLIKAECHARLNQILEAQTAVNTLRAKRINNSAPANVINLSFSNKEDAIKKILEERR
                     RELAFVQRWYDIRRLNNNDDPNDDVVMTKTFYGYNSSSILNNEGLKIYTLPKDSRRFA
                     TPIPYTDIVAGHGVLKQNTYDNNSVLVQ"
     gene            21145..21807
                     /locus_tag="P5293_00805"
     CDS             21145..21807
                     /locus_tag="P5293_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48506.1"
                     /translation="MKTLNLLISLFISCVVLAQNRDLTITVKNNSGKPIKFVKVETFP
                     SNLKGSTDKNGLCILKGISDDDSLIVIYPNADKSATYSLKGLKEIQFNPSLNNPVGYD
                     PIGDLQIFGKERKIVKKGEIDLENEIKNGALNLEDLLKKMPSLMVTGGEISFRNPVIT
                     KALKEVSPLIVVDNMIVRGGLQEANRIVNIHMIESIKLEKDGTLWGPEGLNGVILITL
                     KK"
     gene            21851..23371
                     /locus_tag="P5293_00810"
     CDS             21851..23371
                     /locus_tag="P5293_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HRR48507.1"
                     /translation="MKHNLLIILLLMPIMAICQNNQRINKNVDQDSLMQLKSCVEKNP
                     NDIDAHNKIIKYLGIENPDLLVIYEAWLKKFPKSAVVPFAIGEAYANAESPKAKPWLL
                     KAIAIDSTMAKAYFNLWLDGERWGDFIGAREYLKKAMEADPTNPDYAFYYRSGYKDID
                     TNLYRQGMLEMPKLFPTSERGAQALYWLGIFVKSPKEKEAVYTQLKNTYPPDKFRWSS
                     SGMYNFFYFYLENNPNKALELAKYMLSISTEDRDKKYWNDCSQLADNFAKAKNLISEK
                     KYREALDVIESCKPGRYSQAIELINFMKAETLDALGETQKAYNQLIKYYASTPGDNVW
                     KIIDTYGKKLKKNSNTMKADLWKIWDSIAVPATPFCLEQYMKPGKACLSDFKGKVVLL
                     TYWFPGCGPCRGEFPHFENVIRKYSKDKVEYLAINIAPEQDEYVVPFVKSSGYSFIPL
                     KDEPDKRGNLTAVGAPTNYLIDQNGRIVFKNFRIDADNERMLELMINELLDHTTIK"
     gene            23383..24504
                     /locus_tag="P5293_00815"
     CDS             23383..24504
                     /locus_tag="P5293_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR48508.1"
                     /translation="MKQIAFTISLLLIFLNSFGKTNIDQNIDNKGVIFENLTLQEALN
                     KAMQNKKEPKMVFVDCYTSWCGPCKYMSTNIFPKEVCGTFFNSNFINIKIDMEKGEGP
                     EVAKKYSVSVYPTFLILDCKGQEINRVIGSDEAEPFINKVRMAMNPLSSPKARFEAYN
                     KDSSDSNLYLYIKSLKEAYLIKELGLFIQNKFFSWTPEVRYSKEIWEALISPTGALSD
                     TKNEIFKYILNNRFEIDKYIGKEKVDEQLLKTFKVYMMRYISGNLPDEKENDININIV
                     CANAISNCDFGIETLVKMVRLKQENKIEDVVKMLSFTKLYRSNALDQEMIEKSVTYIK
                     DLTPEQKTIVSKYYNDKSEYLTRDAAYAKRTAEKLVEVK"
     gene            complement(24817..>24961)
                     /locus_tag="P5293_00820"
     CDS             complement(24817..>24961)
                     /locus_tag="P5293_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48509.1"
                     /translation="SLGVKNIHLGPTLPGFLSSNVANVLVKNFGIAPIGTVEDDIKLF
                     MEA"
BASE COUNT         8763 a         4505 c         4501 g         7192 t
ORIGIN      
        1 tatgtttaac ccctgccttg aacttttgca taaaatctgc ttctccaaac tcttttgact
       61 taaaattaat tttattgtca agagtggcat tataagcaaa ctgtatcttt tcgtaaaatt
      121 tttccttgcc tgctgcctct tttctcttga aagggtagat tcggtttacg ttaaaagtaa
      181 aattaggaaa cgtaaatgca taagaactgt caagagaatt ctgactgtga aggaggttag
      241 cagaaaagct gaaaggagtc cctgcaaaag ttctggaata agaaacgctt gaagaaatct
      301 gattttgcag agtctgctgt atttcacgag agttatatcg attgtttgaa ggagaagaaa
      361 agttgacaga cgctctgaaa gatgtaccgg gatgagcttt ggaatcttgt gaatgtgtcc
      421 attttacggc aaaatccttt gtaacataat agccttccgt gccaggctcc cccaccttgt
      481 ttcttgaata attcagacta aaacctccat caaatttata tcttactttg tatcttgact
      541 ccatctggat attgtacgac cccaaagaaa acagatctgt ggtgacagta aaatctaaat
      601 gatctccaaa aacaaaatag tatccgagtc cagtcaatcc aaaacctctt gctgcctctt
      661 ctgtgacaga aggaatgagc agtccactgg cccttttagc ctgtttagga acgaatccaa
      721 aaggaagggc aaagggagta ggtacatcct ccacaacaag ataggcaggt ccaaagacag
      781 tatttctcgg ttcattaata actcttgccc tggtaagctt aagatagaaa tgtgggtgat
      841 ccagctcaca ggttgtatac ttacctttat ttatattgat agagttatca ggcattttct
      901 ttattacatc accatggata tatccctcag actcctgtgt aatcatattc tttattttgg
      961 ccttttggct tttaaagttg taatggacat tttccataac atattcactc ttaccctctt
     1021 tcatctttgg caatccaatt acatttccgg aagtatctat attccctctt gcaaaaactg
     1081 tctgagaatc tatgttatat tccatataat cagcagtaag ctccaaattg ccatatttca
     1141 ccttaacatc tccataatag tatataattt ttctgttatt agaaaaatcc tcaaccactg
     1201 aatctttcgc ttcagaaaag agcggcattt ttattgatcc tttgggtgca tttaacgaat
     1261 cattggataa agaatcaact ctcaccgagt cactcttttt tatcaaagtg tctctttcag
     1321 ctggcatatg actcattctt ccaggtgaag gtattgaata aagatacccc gcaaagagag
     1381 taaaaaacga gccccacagg attctgttga tattatgtcg aaagcccaaa tgggttaaaa
     1441 gtttaaataa attatactac ttttgtgaat tgcaaagata aattatttta agcaacaaga
     1501 atgccacaca ggttactttt atcatttttt tgtttgcttg ttctgtcaac acctcttctg
     1561 gccacagacc cggtcaaaat aagaaaagtt gttatagatg caggacatgg cggtcaagac
     1621 cccggagcca taagtccaaa ccgaaaagtc ttggagaaag acataacatt gtcagtagca
     1681 ttgaaatttg gcaaattgat agaagataaa aatcctgata ttcaagtgat ttacacacga
     1741 aaaactgacg tttttgttcc actgaacaat agaagcgaaa ttgcaaataa aaatcaggca
     1801 gatcttttca tatcaataca tgtcaattct gtaaaggcaa gatcagcttg cggctctgaa
     1861 acatatgtaa tgggtgaaag caaaagcagc tcaaatctcg aggttaccat gatggaaaac
     1921 agcgtaatag catttgaagg agacagtaag tcagtctatg aaggatttga ccctaataat
     1981 cccgagtcat acataatttt cagtttactg caaaatgcac atcttgagaa gagtcttcag
     2041 atggcagaat atgttcagga gcaattggcc gaaggaccta taaaagttaa cagaggcata
     2101 aaacaaggag gacttctcgt tttatggaaa acatcaatgc cagctattct tgttgaattg
     2161 ggattcattt caaacccaag cgattatctc atgctttgtg gagaagaaaa tcacatgaag
     2221 tacgcaaaag ctttgtacaa agcttttgaa aagtatcgaa aagcttatga agcaggaaac
     2281 agaactagca tttcaaaccc tactgctact gctactgcta ctgctactgc tactgctact
     2341 gctactacta ctactacttc ttctgctgct gccattcctt ctattcagga ctcctccaga
     2401 gatacagcaa caaaaaatac agcaagtacc aaaaccacaa cttcagataa aacttcaagt
     2461 aaatcaagca cttccagtat atcgtatcca tacagaattc agatacttgc agttacgaaa
     2521 aagttacctg ataatgcccc cgatttaaaa ggggagaaaa atgtaaattg tattaaagtt
     2581 ggaaattatt tcaaatacag cattggcgaa ttcaaaacca gagaagaagc catgaaggaa
     2641 ctgccaaatg taaaaattaa atttccccag gcatttataa caaaagttga aaatgaaaat
     2701 taaattaaac aaagaacaga aaatcggttt atttgcaatt ataatagccg tctcactata
     2761 cttcacaata aactatctta aaggtaaaga catatttgga agcaccaaca cctattatac
     2821 tgtttttgac aatgtagaag ggctaagtgc gacaagccct atatatatta aaggttacaa
     2881 agtaggcacc atccttgaaa taaaatatct cgaacagcaa gaaaagtttc tggtaaccat
     2941 gaaggttgac gcaaaatatc acatacctca aaactcagca gcccaacttt acagttcaag
     3001 tatacttggt ggcaagtcaa tcaggataaa tattgttaat ggtggtaaaa taataggaga
     3061 aagggataca ctgcgttctg aaattgaacc tggtataatt gacaacatag cagctcaaat
     3121 aataccattg aaggaccagg caacagagtt aattgccaat ttaaacaaga ccttcgtcag
     3181 cgtaaacgaa gtcctcaatg atcaggcaaa agagcaactt tcagtatcaa ttgccaatct
     3241 tgacaagaca ctcaaaagca taaaagccat tgcatccaat atcgaaggta aaaatcccga
     3301 aatagcttct atcattacca acctggatac cctgtccttc tcactgaata aaagtgcagc
     3361 agcactcgac aaagggctcg ccgatataac cgaggttact tcatcactca aaaaagcgga
     3421 cattgaaggg actattacct ctctcaaaaa tgttctcata gaattaaaca atccgaacgg
     3481 gtcgattggg aaacttatta aaacagattc gttacacacc tctatcgatt cattggtaag
     3541 agatatagac gtgctagtca aaaacattca aaaagaccct aaaaaataca taaaaatttc
     3601 tgttttttag tacttttttt tgctttattt tattgattta tagtatatca atttttttaa
     3661 aaaatttttg tctgaatcac agagtattga aaaaatcttg aattttcaaa gctaattttt
     3721 attttttttt aaagaaatta tccccatttt tgttttacaa tctaaacaaa aatctctcgc
     3781 atgtctgaag ctcctcatat aaaagtgtgg aataattgtc tgcaaatagt taaggataat
     3841 attccactcc aaagtttcaa gacatggttt gaacctataa aagctgtcaa gctgacggac
     3901 aatgtactta caatcgctgt tcctacccac ttttttgtgg aatttcttga agagtattac
     3961 attgatctga taagcaaggc acttaaaaga gaacttggca aagatgcaaa actcgaatat
     4021 aatgtaaggg tcgtttccaa tgaaaatccc gtacagcttc cgcatcaatc ttctgccgag
     4081 attaccaata aagctcttcc ttttcccaat caggaaaaac agattgccaa tccattcgta
     4141 attccagggc ttcagcaatt aaaaatcgat ccacagctca atcctcactt ctgctttgac
     4201 aactttatcg aaggagagtg caacaggctt gcgagatgtg ctggactcac cattgcttcc
     4261 aatcctggtg caagtgcttt caatcctctt tttctctatg gcggttctgg acttggtaaa
     4321 acccatcttg cccaggccat aggtattaag ataaaagaac tttttcctga caaaattgtg
     4381 ctgtatgtga gcgcaaatcg ttttcagaca cagtttatgg atgctgtcaa cattaaaaac
     4441 aaactgactg attttcttca tttctaccag atgatagatg ttttgattat tgatgacgtc
     4501 caggaatttg ccgggaaaag tggtactcaa aatgcatttt ttcacatatt cagtcacttg
     4561 cacaatcttg gcagacaact tattcttaca tctgacaaac ctcctgtaga tttacaggat
     4621 ttggaacaga gattgctttc aagattcaaa tggggactgc ttgccgagtt aagacaaccc
     4681 gactatcaaa ccagaatggc cattatcaag gctaaatgtt ataatgacgg cattatgctt
     4741 gaagatgaag tggtttcata tctcgcatca agagtaacaa ccaatattcg cgaaattgaa
     4801 gggacattga actcgctgct tgcccaggcc atattcaaca aaagaactat tgacatggaa
     4861 cttgctgaaa gcctcactga gaagattgtc agcagaaaca gaaatgaaat aaccatagaa
     4921 cagattcaaa aaaccgtatg caattatttt aagataaacc ctgaactttt tctttcatcc
     4981 tcaagaaaaa gagaactggt tcaggcaagg cagattgcaa tgttcctcag tcgcaatctc
     5041 accaacaact ctctgtcaac cataggttca cttaccggcg gaagagatca tgcgactgta
     5101 ctccatgcat acaatactgt ttgcgatctc atagacaccg acaaatcctt cagaaaatat
     5161 gtcactgata tagaaaagaa actaaaacct taatttaaat ggattccaat aaaattatat
     5221 catacttttc ggaaatatgc caaataccaa gagaatctgg acacgaagaa aaaatcatag
     5281 aatttctcgc aaattttgca aggactcatg ctcttgaata taaaatagat gatgccggca
     5341 atgtacttat atcaaaaaat gcaactcccg gcagagaaaa ctcaccaacc gtagtgcttc
     5401 agagccattc tgacatggtt tgtgaaaaga acagcaacag caatcacgat ttcagcaaag
     5461 accccatcaa gtttattgaa gaggatggat ggctcatagc caaagacaca acacttggag
     5521 ccgactgcgg cattggtatg gcagcgcaga tggctgttct tactgacgac acattggagc
     5581 acggaaaaat tgaagctctt tttactaccg atgaagagac aggactgaca ggagcaaaag
     5641 caattaaacc cggttttatc acaggaaaat ttctcatcaa tcttgactct gaagacgaag
     5701 gcgaaatttt tattggttgt gccggaggaa tagataccac agccgtgttc aatatacctc
     5761 atgaaaaacc tgcaaagagt tcagaattta ttaaggcggg tttttatggg gctaccggag
     5821 gacatagtgg cgatgatata aataaaaaca gagcaaacgc caatcaactt ctcgtcaggt
     5881 tcttgctccc attattcgat aaaaacatta tcaatatctg caaaatagat ggtggcaaca
     5941 agcgaaatgc catatcgcgt gaggcattcg caatagttgc attcaatccg tcatatagag
     6001 aagccatttt ggagcatttc aaaaaagttg aaaatgaagt taaagaagag tataatattt
     6061 cagatccaaa tctgtttgga aagcttgagg aaacagaacc gcaagcgtgg tgcatagact
     6121 caaatactgc aagtaggatt ctcaactctc taaatgcctg tcctcacgga gttcttgcaa
     6181 tgagccagga tattccagat tttgttgaaa cttccacaaa tctcgcctca gtcaagacat
     6241 ccaatgatac cgttactgtc agcacaagcc agagaagttc tctaaacagc gcaagatatt
     6301 atgcagcaag cagaatagaa tcacttttca agcttgcagg tgcacaagtt acccatgaaa
     6361 gtgaataccc tggctggaag ccaaatgtta attccaaact gcttgaaata agcagacagg
     6421 cttacaaaag acttttcgga tatgaaccag ttgtaagagc tatccacgca ggtttggaat
     6481 gcggtctatt tttggacaag tatccggatc ttgacatggt ttcatttggt cctacactta
     6541 aaggagtaca tgcacccgga gaaaagcttg agattgcttc agtagaaaaa ttttggaaac
     6601 tgcttgttga aatactaaaa aacttaaaat aaagttaata tgggagtact gattgcccca
     6661 tccatgctct ctgcagattt cgggaatcta tctaaagaag tcgaaatggt taataacagt
     6721 gaagctgatc tgatacatct cgacattatg gatggccttt ttgttcctaa catttcgtat
     6781 ggtttccctg tagtcaagtc aattgcaaaa aacgcaaaaa aaccacttga tgctcatctg
     6841 atgattatcg atcctgacag atatgtagaa agattcagag atttaggagt acaatatttg
     6901 agtgttcact atgaagcatg cattcattta cacagatctc tgcaaaaaat caaagacctt
     6961 ggaatgaaag ccggtgtcgc acttaatcct cacacacctt cgcatctcat aacagagata
     7021 gcctgcgaaa cagatttcat ccttataatg tctgtaaatc ccggatttgg aggtcaaggt
     7081 tttattgaaa acagttacga caaagtacgc agaataaaag agttattaag tgacagagct
     7141 tccacatgtc ttatagaagt cgatggagga gtctcaaaag aaaatgctgc atcactggtc
     7201 gaagccggag ctgatatatt agtagcaggg aacagcatat tcggatcaga aaatcccgtc
     7261 aaagcaatta gtgatttaaa acatatttaa tctccttaaa ggcataactg gtgacttccc
     7321 ctcccctcct ttcaagaagc agacatgcgt tttgatcaat gcctactatt tgagcttcaa
     7381 aaatttcatt tgttcctgta tcctcataaa aatggaattc tctcaaccga taaagtttgg
     7441 aaagatattc attatttata gttcctgcca tttgctcata ggcattctga ttgatcatgt
     7501 tataaaatct cttgataaag cttgaaagta ataccagctc atcctccagt ttatatatct
     7561 ttccggtttc aagaaaaagc gaagtaggat ttggcgcatt agaagcaaat tttttttggt
     7621 ttatattcac tcctatacct aaaatagaag ctgacaattt gtcactacct aaataatgtt
     7681 caataagaat cccacaaatt ttattatcca atacatagat atcattaggc catttaatct
     7741 ttgcttccaa acctttgccg gttagatagc tgacgactcc caaagctgca gcttccgaaa
     7801 gtaaaaattg atcttcagcc ttcaaacatg caggcttaac aagaattgaa aatgtaagat
     7861 tctctcccac agcactctcc catgtgtttc ccttctgacc acgaccattt gtctgaaatc
     7921 tcgctgcaaa aacagtaaag tcttccgcac cctccatgcg tctatatgcc tcagaattag
     7981 tcgaatccaa agtttcaaac caaattatgt tatctgacat aaaataaata ttataattgg
     8041 aaacaaaatt gcagtaattt tgtgatatat taaaagatta actaaacaaa caacaataaa
     8101 tagtgccgac aacaaaaatc aaaaaacaat cggggagtga tgcactggag gtacaatgca
     8161 tcgctgatgc aatgctcgac aaaaaaggta agggcgtttg ctcgcttgat cttaaaaaga
     8221 taggtactgc aataactgat tattttatcg tttgcaatgc cgactctccc acacaagtaa
     8281 atgccatagc tgaccatgta gaagacatga tgtacactca atgtaatcgt aaagtcaccc
     8341 gcatgcaggg aaaggagaat tctttctggg taatactcga ttacaccaac attgtagttc
     8401 acatatttca gacagagtac aggctcttct acagacttga agagttatgg gcagatgcac
     8461 caaaaattaa ttatgaagag aaatagaaac aatatggata tcaaaaacaa caataataat
     8521 atcaatagaa acaacaaaat aaagcagcca aaatttaatg ccatatggat ctacgtcatc
     8581 ctccttggag gaattttcta tatgtgggct acatatgacg gaggaaatcc cttaaagacc
     8641 gagtgggttg atgtaaaaga gaagatggct ccttcaggtg atatagaaaa gatatcattt
     8701 atcaccaaca tgaaccgagc tgaggtatat attaaaaagg acagcctggc aaaatacaag
     8761 aataagtttg gaggtaagat tccaaaattt gcacctcaat tttattttgt ggtttcaagt
     8821 aattttaatg ctgaagaaca gatagacatg ctgaggcagc aattgcctga aaacagcaga
     8881 ttcacctttg aaaacgaaga gcggacagac tattttggta aaataataga atggctgctc
     8941 ttccctatca ttcttgtcgc actatggctt ttcatgttca gaagaatgaa taagggaatt
     9001 ggtggtggtg cccagggagg aggaatattc tcagtaggaa aatctcaagc caaacttttc
     9061 gataaggaaa acaatataaa agtcacattc aaggatgttg caggtcttga agaagccaag
     9121 attgaagtca tggagattgt ggacttcctt aagaatccaa agaaatacac cagtcttgga
     9181 ggaaaaattc ctaaaggtgc tcttcttata ggtcctccag gtaccggtaa aactctcctg
     9241 gccaaagccg tagcaggaga agcaaatgtt cctttcttct caatctccgg gtcagatttt
     9301 gtggaaatgt ttgtgggtgt tggggcttca cgagtaagag accttttcag gcaagccaag
     9361 gaaaaggcac catgtatagt cttcatagac gaaatagatg ccgtaggccg tgcgagaagt
     9421 aaaaatgcag gtttttcatc aaatgatgaa agagaaaaca ctctaaatca acttcttaca
     9481 gaaatggatg gatttggttc aaattctgga gtcattattc ttgcggcaac aaaccgtgcc
     9541 gatatacttg acaaggcatt aatgagagca ggacgtttcg acagacaaat ccatgttgac
     9601 ctccctgaac tcaaggaaag aatggagatt tttaaagtgc acctcaaaaa tctgaaattg
     9661 attgaaggat tcaacgtcga atttcttgca aaacagacac ctggtttttc tggagctgat
     9721 atagctaatg tttgcaacga ggctgctctg atagctgcaa gaaaaaacaa aagtgcaata
     9781 gataaacagg attttctcga tgccattgac agaatagttg caggattgga aaaaaggagc
     9841 aaaataattt cacctgaaga aaagaaaacg attgcctacc atgaagcagg acatgctact
     9901 gtcagttgga tgttgccaaa tgccaatcct cttttaaaag taaccatcat tcctcgcggt
     9961 aaggcacttg gagcagcatg gtatctgcca gaagagagac agataacaac aaccgaacag
    10021 atgatggatg agctttccgc aacacttgga ggaagagcag ccgaagaaat cattaaccaa
    10081 aaaatatcct caggagctct taatgacctt gaaaaggtga ctaagcaggc ttatgcaatg
    10141 gtttgctatc tgggaatgag caaggctata ggtaatgtat cattctatga ttcttccgaa
    10201 tcgagtggtt tcactctcag caagccatat agcgaaaaga cagcagaact tattgataaa
    10261 gaggttaaaa agttcatcga cgaatcttat gaaactgcta agaaaatttt aaaggaaaac
    10321 atggaaggat tcgtaaaact tgcagagcaa ctactcgaaa aagaggtaat attctcagaa
    10381 gatcttgaaa aaatctttgg taaaagagcc gggagccagc caccttcaag agaagatgac
    10441 acaacttcaa cctcatccga gaaatgaccg gtcgttttaa aggaacattt gtcagaagca
    10501 tcaccggcat tgttttcata acaataatgg tgggatcgat gtttttaagc cctgttgcct
    10561 ttggagcagt aatgactata gctattgtct ttatgatggc agaatatatg atgatagctg
    10621 tagatgtaaa attgagcttg acaaaaatcc ttaccattat agcagggatt atgctttatg
    10681 tacttttttt tctgtacaag gcatatggaa tagatgccaa atggctctat ctgcttacaa
    10741 taccttttgc ttcaattttt gtttcactgc tatgctacag agataattca aaaagtgatg
    10801 gcacagcaac agatcctgaa gtatacaaca gacatcctta ccttcttaca tcattgatat
    10861 acattgctct gccattttca atgacctccc tgattctctt tgattcagac ttgaattaca
    10921 gacctctaac tttgctgtca ctttttatta ttctttggac atccgatgtc ggagcatata
    10981 cttttggaat gctttttggg caaaaaaatg gacataaact ttgcccttca atatctccca
    11041 aaaaatcgtg ggaaggattt tttggaggta ttttctgcgg attagtagca ggtatagttg
    11101 tttggatttt gtcactaacc ggattttcat taacacacaa tataattata gcattgatca
    11161 taagccttgc cggagctgcc ggtgatttgg tagagtctca gtttaaacgt aattttggtg
    11221 ctaaagattc aggaaagctc ttgcccgggc acggcggttt gctcgataga tttgatgcag
    11281 cgctcattgc gtttccattg gcaatcactt acataaaatt attttcttta atataaaaga
    11341 tcatggggat acataaagaa ggacgcggaa taataactaa aagttttttt ctgcttgctg
    11401 ctataattat tgcactcttc ttcataattg gtcttgttac tgcattcatc atttcagcaa
    11461 tagtagcact gattattttt ttcattatca ttagattttt cagatctccc aaaagaactc
    11521 ctattacaga agaaggtgca gtgatagcac ctgcagacgg aagaatagta attgtcaagg
    11581 aagtctatga agatgaattc ctgaaatgca actgtattca ggtatcagta ttcatgagtc
    11641 tgctagatgt tcatgttaac tggtttccag tatcaggcca gataaagtat tacaaatacc
    11701 atccgggaag atacatcata gcaatgcacc caaaatcatc agacaaaaat gaaagaacat
    11761 caattgttat agaagaaaac ggttttcctg tcctcttcag acaaattgca ggatttcttg
    11821 cacgaaggat tgtatgttat gctgttgagg gacgagaagt taaaaaaggt tctgaaatgg
    11881 gatttattaa atttggttcc agagtagatc tgtttcttcc aatggattca agaattcagg
    11941 taaccaaggg tcaaaaagtc aaaggttgcc aaactgttat agcaaggctt ccagagttga
    12001 cagaggcaca gaaatttctc tacaataaca ataaaaattc agctttttat taatctttaa
    12061 ggaaaagcga catctctttt gctgcctcag cagctttaga agcagctgca tcggcaaaat
    12121 ctttgccttt gcctgcataa ataatatctc tcgagatgtt taccagcaga ccgcaatttt
    12181 tgttcatgcc atatttggcc acttcagcga ttgttccacc ctgtgaacca acccccggga
    12241 caagaaagaa atgatttgga cagaatatcc ttatttcagc tagcttctga ggtctagttg
    12301 caccaacaac atacataata ttattaatat tcccccacct catggatttc tcaataacac
    12361 tcttaaacaa agggaaacca accgtctcag ttgtagagta gaagaaccct tcactgtcgt
    12421 acatggcaga aagatcatcc aacatctgaa tatgcccaag cagaggcaaa gtttcaaaat
    12481 catctgcgga aacattggat gttaaagcaa gcactgcagc ccactttccc gaatattgaa
    12541 gaaaaggcat tattgaatcc ttgcccatgt aaggagagag cgtaacagca tcaaaatcca
    12601 ttttttcaaa aaaagccttc gcatactttt cggacgaatt ccctatatcc cctcgtttag
    12661 catcagcaat tacaaacaat gattgatctt tctttcttaa atagtcaaca gtcatctcaa
    12721 gttgccgcca gcccactgca ccttcagctt cgtaaaaggc cgtatttggt ttatatgcaa
    12781 cagcatacat agctgtagca tctattatcc gtttgttaaa ttcaaaaaga ggaaattcta
    12841 ggttttttaa ttggtcaggt atctgagcag aatcagggtc aaggccaaca cataaaaaac
    12901 ttctcttttt aattatgttg ttatacaatt caagataagt catccaatta attgttttaa
    12961 gtcagttatt gaaatttaaa tgttattttc tccggaagcc tttagtcgtt ctgcattctc
    13021 agcaatctga agctgatcta tcaactcctg aatgttacca tccataaaag aaggaagatt
    13081 gtacattgta taattgattc tgtgatcagt cactctcgac tgtggatagt tataagtacg
    13141 tattttggca gacctgtctc ctgtcgaaac catagtcttt ctctttgcag ctatctcatt
    13201 taaatacttc tcatactcaa gattatatag acgagttcta agttcagcaa gagccttgtc
    13261 ataatttttt agctgcgact tctcgtcctg acactgtaca actatgccgg taggaatatg
    13321 tgtaagcctg atggcagaat atgtagtatt aacagattgg ccaccaggac cggaagagca
    13381 aaaagtgtct tttcttatat cattgatatt aaggtctatg tcaaactcat ccgcctcagg
    13441 caaaactgca actgaagcag cagaagtgtg aacacgcccc tgagtttcag tttgaggaac
    13501 tctttgcacg cgatgtacac cactctcata tttcaataca ccatagacac cctgacctga
    13561 aatagtacaa atcaactcct taaacccacc agatgtaccc tcgctcttac ttgtaacttc
    13621 gagtttccag cccttagatt cagcgaattt cgaatacatt ctgaaaagat ctccagcaaa
    13681 aattgaggcc tcatctccac ctgctcctgc acgtatctcc aatatggcat tcttagaatc
    13741 ctgaggatcc gctggtatta aaagtaactt tatctcctcc tcgacaacag gaattttctc
    13801 ctcaatggca gaaagctcct ccttgaccat ttccctaagc tcttcgtctt tttctgtcaa
    13861 aagcatttcc cttgctgatt cataatccct tagcagcttg ccatatctgt ctcccgcctc
    13921 aaccacaggc tcaagttcct tgtattcctt gtttaacgag acatatctct tcatatccga
    13981 catcacgtct ggatttgaca gttgagctga aatattctca tatttttccc gaaggcccga
    14041 gagcttcgac aatagattgt tatcactcat aaattaaaaa tatcgtgcaa aaatagataa
    14101 attagttgac acttgccaat cataaaaaaa tctatctttg ttaagaataa ctatgataac
    14161 tttaaaatct gtagttttat ttgagtaata atggtgaaaa taagaagaat aattctgcat
    14221 ctgattttga acagatgtat atctccaatt atcataaaat aagatcattc tgcaacaatt
    14281 atcttcatga caatgataca gcaaaaagta tagcacaaga tgttttcatt tctgtttgga
    14341 acaatcgaga taatttaaat ttttcagatg aattgcttcc ttatcttttt attcttgcaa
    14401 agaatatgtg ccttaatgtc cttaaaagag gaaaagtcaa acaaaactat aacgactatg
    14461 aaaaacggca taatcgagat tctctaaatt atgcttcact aaaagagcaa acaataaatt
    14521 cattatatag aaaggaagtt gaaaagctta taaacgactc tctgttaaaa atgccggaaa
    14581 gtgtgaggtc tactttttgc ataagcagat ttaaaaattt gaaatatgag gaaattgcag
    14641 taattcagaa aatatctgta aaaactgttg aatatagaat aatgtatgcg ctgagagtat
    14701 taagaaaaat cctaaaagac tatttgccta tttttttgta gggtattagt taattaaatt
    14761 gtattacaag cagaagaact atatgaaaaa atcgctatta ctaaagtata ttagacgtga
    14821 aacaacaagt ccggaagaag atgatgtcct caaatggatt aataaatctc ctgacaatga
    14881 gagatatttt ataggtctaa ataatttatg gatatctcag aatatctctc aagataaagc
    14941 cagtaaagag gaagttgatg agataaaagc acttactaca aataaaaaaa catctcgctt
    15001 taaatataag aaagttacct actatgttgc tgcagcaata atattaatct cagcaggact
    15061 caattttttc cttataaaag aaaactcact attacaacat aaaattaacg agccaataca
    15121 acttgccgaa cttccatctg cttataaaca tgaactctac acagaaaaag gagtgaaggc
    15181 aaaaattatt cttcctgaca gttcttatgt ttggctaaat tctggttcga aaataatatt
    15241 tccagacagg tttgaacgta acataagaga gatcgaattc tcaggtgaag ccttttttga
    15301 cgttcacaaa gattctttac gtccacttgt tattaaaaca aataaaaatt ttaaagtaga
    15361 ggtcagggga acaaaattta atcttaaaac atatgacaac gacaaagaag ctaggaccac
    15421 actttattcc ggttcattaa aggtgatttc agaagtcaaa ggaaaaaaca tccattcaaa
    15481 agaagttatt acccaactca aaccacttga aacttgcatt ataagaggtt acgagaaacc
    15541 tgttctttta agacctaata acgtaaacga tcatcaagcc tggaaaaatg gagaaatagt
    15601 atttgattcg actccaatgg aagaagttgt caaaatacta gaaagatggc acggaataaa
    15661 ttttgaagtt aaagacaagt atgtgtacaa ctataatatt actgcaaaat tcaaatctga
    15721 gtcaatagtc caaattatgg aaatgataaa atattgtgct ccattggatt attctataga
    15781 cagcacaaac gtttttcttt tcaaaaggaa attatgattt tctgttaggg tatttacctt
    15841 tgatattgtt ttacgactat aacttaaatc aaacaaataa acttatgatg aacagtaaaa
    15901 cagtaccaaa ggtcttagta tgctttatct cttttgtagc aatgttcact atatttctga
    15961 acaacgcata tgctcaaaag ataaacatca acttcaaaga tactcctatc aaagaagtaa
    16021 tcaaagaaat aagccagcaa actggttata attttgtcta ttcaaatgcc ttgacagcta
    16081 ttaatgacaa ggtaagtata agctactcgg cagataaagc tcctgttgag aagttattgg
    16141 atattctttt taatgaaaga ggaatatctt ataagataga taagaaacaa attatccttg
    16201 cccctaaaaa tattgttcct gtaaataata ataatcagcc aacttcacaa acaaaaaatc
    16261 aagatcagga gaggtttacg cttaaaggta aagtgcttga tgagtcaaaa ttgcctttag
    16321 taggcgtaac tgtcttaaat ctttccacaa aaaagggaac atatactgat aacaacggaa
    16381 attattctat tgaagttaag gaaggagata aagttcagtt tattttaatt ggaatggcca
    16441 caaaagaaat aactgtcaca aaaggtctta cagtatcaaa catagaaatg atgtcagacc
    16501 aaataagact ccaagaggtt gttgtaacag gttaccagac tatttcaaaa gagagagtta
    16561 ctggagctgt caaaacaatc aggacagata tgcttgaaaa accatcaact aatattgcac
    16621 agaggctgat tggcacagct gccggtgttc aagccacatt ggatgtagat ggtaacccaa
    16681 cttttttgat aagaggtttg acatctctaa atgcaacccg cgaaccatta gtagtaatag
    16741 acggcttccc tacagaaaag gggttcaatt caataaatcc gaatgatgtt gaaagcgtta
    16801 catttttaaa ggacgccgcc gccgcctcca tatggggagc aaaagcagct aacggtgtaa
    16861 tagtaattac gtcaaaaaaa gctaaaggtg aaataccttt aagaatagaa ttcaatgcat
    16921 tttacagagt tgcacctaaa tttgaccttg actatgtaag gccgatggca aaatcaagtg
    16981 atataattga ctacgaactg ctctctttta ataaatgggg tgcaagagca tataatgtag
    17041 gaaattttct ggatgggtat tatagaacat acactccaac tcaagagctg atatggcaaa
    17101 attacattgg agctatatct gaatcaaaaa tgaatgaaga aataaacaag tacagaaatc
    17161 tcgataactc taaacagata aaaaaacatc ttcttgataa cccaagtacc cagcagtaca
    17221 accttactat aatgagcggt acacaaaaga tgtctaatta tttttcactt ttgtatgagc
    17281 agaacaactc taattataaa aacacaaaca acaacagaga agtaataagc tatagaaatg
    17341 tagcaaaaat tacaaaatgg cttgattttg aattcggggc gactttagcc tatcaagtca
    17401 acaataataa tgggattaat ctgtcaaaca ttcagagttg ggctcctcat cagatgcttt
    17461 tggacgaaaa tggtcaatat accaatatcc caaatggcat gaactactta ccggcaatga
    17521 caactgtacc ttttgataag tttccttata aatttacata caatcctata gaggagataa
    17581 ataaccgaag aattaccaca aagtcattag acacacgtat tacaggagct ttgtttatta
    17641 aaatatttga tggactgtcg cttgagtcga gagctcaata cgagtacaac aacacattca
    17701 acaaatcgta ttacaatgaa aatacatata ctgtaaggtc tgctgtaaat gcagctgcaa
    17761 catgggacaa aacagtcggt actgttactt tgaaccttcc aaaagggggc tttcttgatc
    17821 agaatcgctc agaagtaaaa gctcttacag tcagaaacca attaaattat cgaaagacaa
    17881 ttggaaaaca tgatttaaca gccctagcag gtattgaact aactgataga gtatcccaaa
    17941 catatgggta tccaagaaca tatgggtatg atgataataa attgaccgta ggcactttcc
    18001 ctaatggacc aggtggttca tctattaaaa ttataaaaaa ctggattgac aataaccaaa
    18061 cattcagtta tacaaacagt tttagttact caactcgcag atatttctcg agttatgcaa
    18121 atgcctccta tacttacgac tcaaaatatt cactcagtgg tagtatacgt tcggatgcct
    18181 caaacttcat cacagatgac cccaaatata gatatgctcc attctggtct attggtgcaa
    18241 gttggaatgt taataaagag aattttatgg ctaacgtgaa cttcattgac cgactgactg
    18301 ccagattgac ttttggatat aatggtaatg ttgatgtaac tacatcgttc caaccactta
    18361 ttacaccaag ttcaagtgtg aatatttaca cacaagaaaa tacaactgct atttcaagta
    18421 tgggtaaccc aacacttcga tgggaaaaaa ccggaacatg gaattttggt attgattact
    18481 caatattgaa aggaaattta tatggctcaa ttgatcttta tcgtaaatat acaagagatc
    18541 taattgcaac catatctatt ccatctgtaa atggaagtac ttcttcaaaa atgaacaatg
    18601 cagagatgag taataaagga attgaaatag aaataggaac aactcaaaaa atatatggtg
    18661 acaatatttg gtggagagga actcttaatt ttgcctataa caaaaatgaa attactaaat
    18721 tatttgtttc acaatatgcc tcatatgact tatatggtgg ggcttacgta gaaggctaca
    18781 atgccaatga actatggtgt ttcaaatatg caggcattaa gaacgaaggc tcagctgaac
    18841 atccaaattg gcagcctcag gtacaggggc caggagatgt aacttacaat tttacaggat
    18901 ggacaccggg tgatgcacgt aactacatgc ttcatatggg aaccactgtt gccccatata
    18961 cattaggttt tgttaatgac ttcaaaatat ttgacttcaa tttgtcattt atattaacag
    19021 gaaagtttgg gcatgtattc aaaagactat cttttaatta tccagtttgg tggaatggtg
    19081 cggtaattcc taatcaaaaa tattctgaag tagtaaatgg cgatccaatg aaaattgtac
    19141 cattaccact gaataatgat gagccaagat tctacttctg gaatagattc tatccaaact
    19201 taagttatct ggtcgaagat gcatctcata taagaatgca agaaataaac ctttcttaca
    19261 acttaccgca gaacttattg caaaaaatgg gactaagcag agctcaactc tatttacaat
    19321 gcaatgatgt atttacaatt tacgccaata aatttggtga ggatccagaa tatcctattg
    19381 ggtcacaaaa tcctcagcct aaatttattt ttggactaaa gtttgactta taattaattt
    19441 aataattgtc gaaaatgaaa tacaaaataa atattaagat attaaatatc ttgttgttac
    19501 tatttattct tccatcctgt tcaaagattg atgatttttt ggatgaaaga ccaagtaaaa
    19561 gtacttctaa accattggaa actgttgaag aactggatgg aatattgggg gcttacagta
    19621 gcttctacac agaaagttct gctactttgt ttgccggcca tgatgatttt ggtctaatta
    19681 aagaaataca cgattcatat gcaggaatat atggaggaac ttctatggca taccacttct
    19741 atccggcagt ctgggatata aaaaatttag agatatccgg tgataaagga tgggctgaaa
    19801 gttggagtaa aatattcaag gctaatatgg tactaaataa tatagataac gtaaaaggaa
    19861 gtgagtcctt gaaagcagac ttgaaagcag aggcgcattt tataagagct tatgaaatgt
    19921 atatgctggc aaatgtcttt tgtttgcctt atactaactt caataaaagt aataaagatg
    19981 aattgggatt acctcttaaa caatcaacct cttttgaaga atccgttgag agaaaaacaa
    20041 ttgaagagac ctacaaattt attgaagcag acttgcaaga ggcattgaaa ataaccacac
    20101 ctttaattag acagagtgat ggagttttac gtgcatggag aggaaataaa actggagtta
    20161 aagcatttgc agctcgatat tacctgtacc aaggagatta tgaacaggct ctccattacg
    20221 caaatcttgc tcttggagaa tataatactt tagtcgatta taacctgaac agcaaaaatg
    20281 gtggtatgac ttgggggctc ccaaaagctt atacaataaa ccaaggaaca cctcaacaac
    20341 aaacagtaac attaaaatat ccttatggcc atgataacca gtcagatcct accgatagga
    20401 tggcatggaa ggaacatttg tatatgagat tcatgtacaa tggatcttgg tggtttattc
    20461 caagcagaga gctgctcgca ctgtatgacc agaattatga tagaagatat gaatacaatt
    20521 acgttcaggg ctattcctat gatcgcggat gcgttaaacc tgcttatagt tatccaggat
    20581 acatacgttt ctttaaggat aaccttccaa gtggtccaac agtagctgaa atgtatctta
    20641 taaaagctga atgtcatgct cgccttaacc agatactgga ggcacaaaca gctgtcaata
    20701 ctctcagagc taagagaatt aataattccg ctcctgctaa cgtcatcaat ctgagtttct
    20761 caaacaaaga agatgcaatt aaaaagattc ttgaagagag acgccgcgaa ctggcatttg
    20821 tacaaagatg gtatgacatt agaagactta acaataatga tgatcctaat gatgatgtgg
    20881 ttatgacaaa aacattttac ggttataact cttcttcaat acttaacaat gagggtctta
    20941 aaatttatac tttacctaaa gattcaagaa gatttgcaac tccaatacct tatactgaca
    21001 ttgtagctgg acatggcgtt ctcaagcaaa atacttacga caataattcc gtacttgttc
    21061 aatagccttc aatactccat atatcgggtt aagattagtt taatttaata aatcttaacc
    21121 cgatttgtta acataataca caaaatgaag actttaaatc tattaatatc attatttata
    21181 tcttgtgttg tattagctca gaacagagat ttgacaataa ctgttaagaa caattccgga
    21241 aaaccaatca agtttgtaaa agtcgaaaca ttcccatcaa atcttaaagg ttcgactgat
    21301 aaaaatggat tgtgtatttt aaagggtatt tctgatgacg attctcttat agttatttat
    21361 ccaaatgcag ataaatcggc aacatattca ctcaaaggac tgaaggaaat acagttcaat
    21421 ccttcattaa acaaccctgt gggctatgat ccgataggag atctccagat ttttggaaaa
    21481 gaacggaaga ttgttaaaaa aggagagata gatttagaaa atgagataaa aaatggcgca
    21541 ttgaaccttg aagatctttt aaagaaaatg ccttcactga tggttacagg aggtgaaata
    21601 agttttagaa atcctgtaat cacaaaagca ttaaaagaag tttctcccct tattgttgtt
    21661 gacaatatga ttgtaagagg tggccttcaa gaggctaaca gaattgtcaa tattcacatg
    21721 atagaatcca ttaaattaga aaaagacgga acattatggg gccctgaagg cttaaatgga
    21781 gtgatattga taacattaaa gaaataaaat ttaatttgaa ttacattatg aattataaaa
    21841 acaataatct atgaaacaca atttattaat aatcttactg ttaatgccta taatggcaat
    21901 ctgtcaaaat aatcaacgaa ttaacaaaaa cgtcgaccaa gattcgttga tgcagctaaa
    21961 atcatgtgtg gaaaaaaatc caaatgacat cgatgcacat aataaaatta taaaatattt
    22021 aggcatcgaa aatcccgatt tgctggtaat atatgaagct tggttaaaga agttcccaaa
    22081 atctgcagtt gtacctttcg ctattggaga ggcttatgcc aatgctgaaa gtcctaaggc
    22141 aaagccatgg ctgcttaaag caatagcaat agattctaca atggcaaaag cctattttaa
    22201 tttatggctt gatggagaaa gatggggtga tttcataggt gccagagaat acctaaaaaa
    22261 agcaatggaa gctgacccaa caaatcctga ttatgctttc tactatagaa gtggatataa
    22321 agatattgac acaaaccttt atcgtcaggg aatgcttgaa atgccaaaat tatttcccac
    22381 aagtgaacgt ggcgctcagg cattgtactg gctaggaata tttgtgaaaa gccctaagga
    22441 aaaagaggca gtttatacac aattaaaaaa cacatatcca ccagataaat ttagatggtc
    22501 ttcaagtgga atgtataatt ttttctattt ctaccttgaa aacaatccta acaaagctct
    22561 tgaacttgct aaatatatgt tatctatatc aacagaagac agagataaaa aatattggaa
    22621 tgattgttct caacttgcag ataattttgc taaagctaaa aatttgattt ctgaaaaaaa
    22681 atacagagag gctttggatg tgatagaatc gtgtaaacct ggacgttatt cacaagctat
    22741 agaactaata aatttcatga aggcagaaac attagatgct cttggtgaaa cacagaaagc
    22801 ttacaatcaa ctaataaaat actatgcatc aactccgggt gataatgtat ggaagatcat
    22861 tgatacttac ggtaaaaaac tcaaaaaaaa tagcaataca atgaaagcag acttgtggaa
    22921 aatatgggat tctattgcag ttccggcaac tcctttctgc ctggaacagt atatgaagcc
    22981 tggaaaggca tgtctgtctg actttaaagg aaaagttgta ttacttactt actggttccc
    23041 gggatgtggt ccttgtagag gtgaatttcc tcattttgaa aatgtcatca ggaaatacag
    23101 taaagacaag gttgaatatt tagctatcaa cattgctcca gaacaagacg aatatgttgt
    23161 tccatttgta aagtcaagcg gatacagttt catcccttta aaagatgaac ctgacaaaag
    23221 aggcaattta acagctgtag gagctccaac aaactattta attgatcaaa atggacgtat
    23281 agtatttaaa aatttcagaa tagacgctga taatgaaagg atgcttgaat taatgataaa
    23341 tgaattgtta gatcatacta cgattaaata actaaaatga aaatgaaaca gattgcattt
    23401 acaatatcat tgttgttaat atttttgaat tcattcggaa agacaaatat tgatcaaaat
    23461 attgataata aaggcgttat cttcgaaaat ctcaccctac aagaggcttt aaacaaagct
    23521 atgcaaaata aaaaagagcc taaaatggtt tttgtagact gctatacttc ttggtgtggc
    23581 ccatgtaaat atatgtccac taatatattt ccaaaagaag tttgtggtac attttttaat
    23641 tcaaatttta taaacattaa gattgatatg gaaaagggag aaggtcctga agtcgctaaa
    23701 aaatattcag tatccgttta tccaactttt ctaattcttg attgtaaagg tcaggaaatt
    23761 aatagagtga taggaagtga tgaagctgaa ccttttatta ataaggttag aatggcaatg
    23821 aatcctcttt catcacccaa agcaagattt gaggcctata ataaggatag ttccgattct
    23881 aatctttatt tatatatcaa atccttgaaa gaagcatatt taattaaaga actaggatta
    23941 tttattcaga ataaattttt ctcatggact cctgaagtca gatatagtaa agaaatatgg
    24001 gaggcactta ttagtccaac aggagcgttg agcgatacga aaaatgaaat attcaagtat
    24061 attctgaata atcgctttga aattgataaa tatattggaa aagaaaaggt tgatgaacaa
    24121 ctacttaaga catttaaagt atacatgatg agatatatat ctggaaatct gcctgatgaa
    24181 aaagaaaatg acataaacat taatatcgta tgtgccaatg caatcagcaa ttgtgatttt
    24241 ggaattgaaa ctcttgttaa aatggtcaga ctaaaacaag aaaataaaat agaagatgtt
    24301 gtaaaaatgc tctcttttac taaattatat agatcaaatg cactagatca agagatgata
    24361 gaaaaatccg taacatatat taaagatctt acgccagaac aaaaaacaat agtatctaaa
    24421 tactacaatg acaaatctga atatctgaca agggatgctg cttatgcaaa gagaacagct
    24481 gaaaaacttg ttgaagtaaa ataaaaattt aatgcaatga tggagttacg acatggaagc
    24541 agtgattgca tataaaaaaa ggggtgtcca aaaagcaatc cgcaaatgga aagcttaact
    24601 ataggcacat ctgtttttta gtttttttct gaagagtttt ttcggagaaa ggctgaggat
    24661 gtccgacgac catagggaga agttccgcaa gccccgaaaa aatcaaagaa aaagcgttaa
    24721 aaaaaacagc agtgcctata gctaagcttt ccatttcgat ggttgcttat tatataaaaa
    24781 cagaggctgt ctacttttta gacagcctca aacacatcaa gcctccataa acagctttat
    24841 atcatcctct actgtgccta taggtgcaat tccgaagttt ttaacaagaa catttgctac
    24901 attagaagaa agaaaaccag ggagtgtagg accaaggtga atgttcttaa caccaagaga
    24961 g
//
LOCUS       DAOTQL010000007        24679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2108_length_24679_cov_5.253533, whole genome shotgun sequence.
ACCESSION   DAOTQL010000007 DAOTQL010000000
VERSION     DAOTQL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24679
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_24679_cov_5.253533"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="P5293_00825"
     CDS             complement(<1..762)
                     /locus_tag="P5293_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HRR48510.1"
                     /translation="MQPIIKSKSWESYQKHIAEDNYYFARSCIRQNFFPAAEGLFLDI
                     MHNDLHKDIYDDARQTTCTGIAYHSGIIPFETTMGVIARQFSLMTEAGYENFVVSCVT
                     SFGLYTETLETWKEFPEKEREIRDALLRATGRTFEIPKNLVHASDLIYKFRKEISSKL
                     KYRLVNNKTGQPLNVVDHIGCHYAKIFPKEGIGGAEFPYVLAGLIDEWGGSQVDYPER
                     RHCCGFGFRQYLLKSNRSYSVSNTKKKLDSMEPYHP"
     gene            complement(768..1448)
                     /locus_tag="P5293_00830"
     CDS             complement(768..1448)
                     /locus_tag="P5293_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR48511.1"
                     /translation="MELLNELKKDFRFVEGFKACISCGTCTAICPAAEFRDYDPRIIT
                     EIVQRGNENELEKLLKSDTIWFCGECLSCKTRCPRGNTPGYIIQALRALSIKTGFFVC
                     SEQGRKQLAIKRTVGDHILKYGYCVYLDEVDTGMYPEQGPVWDWLKENRFEVLEKLST
                     NYNKTGKGTLRLTSEESLSDLKNIFDETGGTERLNLIEEYSQKAAEKMGIEFSEGKDE
                     YFMELYEK"
     gene            complement(2256..2792)
                     /locus_tag="P5293_00835"
     CDS             complement(2256..2792)
                     /locus_tag="P5293_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRR48512.1"
                     /translation="MKHQIVTTSHGSLLRGIIALIIGGLAVFMPDITLRSFVIYIGIL
                     ILISGIVSLVFSIRSEEKSSKNILMADAIFNLIFGLLFVLLPATMVNIFVIAMGIGFL
                     LIGIIQLIATLSIRRDYGWSWIYFAISMLMIIAGIILFTNPFTSAQTILIFIGIFLLI
                     YGVSEVYMAWKLSKKPTV"
     gene            complement(3422..5593)
                     /locus_tag="P5293_00840"
     CDS             complement(3422..5593)
                     /locus_tag="P5293_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR48513.1"
                     /translation="MKKKLLLIILSLCVLQQFAQADEGMWLINAVDKQLATKMKNKGL
                     KLAPNEIYNENGGALTDAIVALDGGSCTASIISPEGLLITNHHCAYGDIQAISTPENN
                     YLENGFWAMSRDKEIPIKGKTVTFLRKVLDVTDETNRIIDSLDKIGPRGIFFMRKVSG
                     VLEDRYKSDFEVSLASMWRGSKYYLYFYETYSDIRLVGAPPVSVGAFGGETDNWGWPQ
                     HKGDFAIYRVYAAKDGKPAEYSEENVPIKANRYLKISAKGYKPDDYTMIIGYPGSTNR
                     YMSSFQLKQKQEILNPAIYTVRRAKLDVWEKYMEKDPAIRLMYADKYFSISNYTDYAK
                     WENKCLERFNVQQVLEEREKELAEWIAANPERQKKYGNVLSDLKKGYELTSDIVKITE
                     YFRESMIRGSEIIGIGQRFSSLINSMTRAKQDSIVPGSKELISFMQSSDKIFSQCNIE
                     ADKELFKVMLGYYLKEVPRSFYDKDFIKLADSFGCDASKIADYIFDKSVVTNPDRLKE
                     FFSKKVSIKEMETDPILIIVNTSRIIEYNRTESKILDDAGLDMPSLRTAYVHALYEMQ
                     KEKGIAVYPDANSTMRLTYGEVGSLRPADAVYYHYQTEANGILEKYNPKEYDFKLDSK
                     VKGMLEQRDWGMWGEKGNLYIDFLTDNDITGGNSGSPIMNGKGELIGLAFDGNRESMA
                     GDVYFKEEYNKCVNVDIRYILWVIDKFAGAGYLIDEMSIVR"
     gene            5803..7770
                     /locus_tag="P5293_00845"
     CDS             5803..7770
                     /locus_tag="P5293_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR48514.1"
                     /translation="MKKFKKNWLRHLLQWGTLLAIIVFLTKVFGNQTADPESYCPMGG
                     LESLSTYLVSGSLACSMSMAQIMMGIVLALTVVLFSKLFCGYLCPLGWATEYLGKLRA
                     KMKIKEIVIKNGSVLDRVLRLVKYALLFILFYFTITNSELFCKNLDPYYAIATGFKGE
                     ITLWMTITAIALLVLGSFFIKMFWCKYICPLGAISNIFKFTITFVVLVGIYAILGISG
                     LSISWVYLLAATCLIGYIWESFYLESKVFPLLKVHRNTETCNNCGLCAKKCPYSIDVD
                     KVENVKHVDCTLCGECVASCDKGALTFGKKKGLRWLPAILTVILLAAAFFLGTMWELP
                     TIDVTWADQSKIEQLQKTEIDGLRSVKCYGSSMAFKAQLEKVPGVYGVATYVKKHQAV
                     ILYDPSETNPDKIKEAIYTPGKFKIATPPVGAQIKVVTIYTEKMYDKMDPNYLGMQFR
                     LTKKGYYGLETEYSCPLTVRLYMDVNEPVDESFIKSIVEMKELAMPAQNGETNIIKVD
                     YKYEGMSDQIDTISRREFLERQMYKFDKTFKDNYEKWGGKDEAVYEIVYENLDKPIIT
                     RYIPYLASHLSMIDGFLGLAVVLNDNEDYAFRITYSKAVLDDDKIWAALTQPKWKVKS
                     KDGEISETDPKFSFETKGHTIVIKPNDVKSK"
     gene            7794..9965
                     /locus_tag="P5293_00850"
     CDS             7794..9965
                     /locus_tag="P5293_00850"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR48515.1"
                     /translation="MKNNIITRFLVILFLLSVSGISRADEGMWLVNLLDKNLTQQMKK
                     TGLKLDPKLIYDESGSAVSNAVVALDFGCTGSIISDKGLLITNHHCAYSDIHALSTPE
                     KNYLEDGYWAMNISQEIPIKGKSVFFLRKVTDVTDEIKQISDSLKKNNIPGGMRKITN
                     IIETKHSKQSGMETSCNSMWRGSKYYMFYYEVYTDIRFVGAPPVSVAAFGGETDNWEW
                     PQHKGDFALYRVYGNRDGKPAPYSVDNVPISPKKVLPISTKGIKDGDFTMILGFPGRT
                     NRYSSSFSIHEKEKVTNPISVKIMREKLDILNKWMKSDPNIRLKYADYYFGISNLQEM
                     LEGEVMNFRRFGVEKIFQEREKELQQWIDSDSQRQAKWGGLLQKLSTRYNGTEEITRI
                     KKYYQNTLVGGYGLIAIANKAGSLNNSSNRDNLDTLKVGQREYVNFIKEIEKIYKECD
                     MRAEHEMLKYTLKTFVDNVPPLYRGDYINGLAKQFNNDGEAMADYIFNNSIFTNPEKF
                     RQIVKNDQPVSVFGEDPLNLMSKSIGVRPFNEAISKICKGESIDQLEAEYGRVVYQMN
                     LDKGRNQYPDANSTMRLTYGTVGPLDPSDGIHCKSQTTTQGLWDKYNPDNYDFNIKPR
                     MLGFLENKDWGNYCEKGKMFINFLTDNDITGGNSGSPVMNSNGEIIGLAFDGNKESLA
                     SDAYFHPQMNKCVNVDIRYVLWIIDKYAGAGYLIDEMHIIK"
     gene            9990..12857
                     /locus_tag="P5293_00855"
     CDS             9990..12857
                     /locus_tag="P5293_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRR48516.1"
                     /translation="MTIFNKILCTLFICMGISSAYAQNGANDKKPFNRSEKLSVDPEI
                     KIGKLDNGLTYYIRRSLNPTGQAEFFIIHNVGSLQENDDQRGLAHFLEHMAFNGTKNF
                     PKKSLLEYFNRIGVQFGYNINAYTSMDRTVYNISAVPTYRSTIIDTALLALHDWSHYI
                     SCEPAEIEAERGVIREEWRRGDDARSRMMKGISKIEQTGSRFAERDVIGLMEVVNNFT
                     PQTLVDYYHKWYRPDLQAVVVVGDIDVNDIEQRIITLFSTIPAAKNPAKREVYTVPDN
                     KEPIIGLLTDPESKSYSSRLIIKIPNLTPEEKATNKLIYREVMKTLIADIMKERTAIA
                     SQSKDAPFRNCVPVFSQINYAKTTFIMTAMPHSPADMLKATKGILNEVERLTYYGAEQ
                     EELEKAVQNAKITFAKNYQKSKKPKNIDWVNLIVENFTRDYPLITPETFYNIAVSCLD
                     NITLEDLNSSIRSMLTKENRVIVLFAPESEKQYLPTKEEILKLCEEINGKDLARFKPV
                     SKRGLNFNVTLSPKNITSIKQLTNKDFKTPIYKQLDSTVEIILSNGTRVIWNEHKGSS
                     NSVQMRALREGGYFTNIDVMDIKFADRYNTLFSVDSLSKDELAKWSFSRNISLRFSPT
                     MRYSTLSGDFDPKKIDDFFKLLYTSLSECTVNKDDFKRSISNAIKNIEQGKSETNIFN
                     DSVSKLRFKTRKRGDDFTKDYLQNVTPEKLVALHEALYKGINGYTFVFSGPMSAQEAK
                     PYLEKYLSNVPENKKLPATNVSRYDERTTGEVYLRYKAKNQKSTKASVTRYYYSPMDY
                     NALNNLNAKFFSTIMSDRYMASIREERGGTYHVGCSSYLEKYPSQIAECSIDFNTDPK
                     LVDDLLQVVQDEMDNFKKNGPTEQEVTAYKLYLQKKFASKNPAEYSWITTISNALMGF
                     PTFEDQEEALLDKITVKSVKKFANDLTKDKNRMTFIFEPIL"
     gene            complement(12923..14134)
                     /locus_tag="P5293_00860"
     CDS             complement(12923..14134)
                     /locus_tag="P5293_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR48517.1"
                     /translation="MNRFLIGFLSVFSFININFVFGQTKGVSLERAIELALLNNLEYN
                     ACKLKVEQSKTLIPTALTIDKATISYSYDSNNLAENNHPLNVYGIEQSFSFPTLYSAQ
                     YKVTRSESELANRELEIVEKQLIKDVTHSYYEIQYNMNKLMRYRVLDTIYDKFIQNVE
                     DRFNRGEAKSLDLFNARAKHQQIEILLNDITYATRLAYKKLRLLINADTLIVVPLSDL
                     KELTILNEDIDSNPGLKYVNQEVNLQSDMLRVERNKLLPDITIGYYNGSNRFVGSKNY
                     PSYEIGITIPLFFIEQKARINAAKIGIEASRKYAEYYKLSLLLKIEELKSELQKYRGH
                     LEYYYTTGKQMSREIEKEARDGYEKGVIDYYSFVESIQNATLIELEYLDWLFQYNSTV
                     LEINYLILESR"
     gene            complement(14297..14707)
                     /locus_tag="P5293_00865"
     CDS             complement(14297..14707)
                     /locus_tag="P5293_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRR48518.1"
                     /translation="MSRPKQCRKIVSPPLMSGFKPFGIPRSMIKEVILLYDEYESIRL
                     LDYEGLLQEEAAEKMNISRPTLTRIYENARKTIAKAFVEGETIIIEGGNVHFGTIWYR
                     CRKCNKLIEGIENHTPCKNCTSYGNDELFQINKD"
     gene            complement(14912..15475)
                     /locus_tag="P5293_00870"
     CDS             complement(14912..15475)
                     /locus_tag="P5293_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRR48519.1"
                     /translation="MFDFLDVYPWLLPIIIFFGRIVDVSLGTLRIIFVSKGEKYKAPL
                     IGFFEVFIWIVVISEIFSRANSMLSYVAYAAGYASGNFVGIIIEQKIAYGVLLVRIFT
                     QKGGSPLVYILSSEGYGGTSISGKGSQAQVDIVETVIDRKSMKKVENLINRYDPQAFF
                     IVEDVRSKRKGIFPKSESLFSRWRQGK"
     gene            15541..15624
                     /locus_tag="P5293_00875"
     tRNA            15541..15624
                     /locus_tag="P5293_00875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15575..15577,aa:Leu,seq:cag)
     gene            16524..17612
                     /gene="hydE"
                     /locus_tag="P5293_00880"
     CDS             16524..17612
                     /gene="hydE"
                     /locus_tag="P5293_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRR48520.1"
                     /translation="MNIDHIEAITGKSKLEAQDLRYLLTLSGDDMKRLFEISSSVKSE
                     YVGNKVYFRGLIEYSNSCVKNCFYCGIRTGMKQLHRYKLTDDEVLEAAKFALQKRYGS
                     IVIQGGERSDNGHVANITRLLQRINRETNNQLHITLSLGEQSEETYRIWKENGAQRYL
                     LRIETSNEDLYKKLHPDDDLHSFSRRIKALEDLKKLGYQVGSGVMIGLPFQTIDDLAQ
                     DLLFFKAIDIDMNGMGPYIEHEGTPLYNYRHLLMSKEERLALSLKMIAVLRILMKDIN
                     IAATTAMQTLAPDGREQALAVGANVIMPNITPVKYRENYQLYDNKPCINEEAEQCVSC
                     LENRIKSIGNEIGYDEWGDSRHYLKKKF"
     gene            complement(17609..18361)
                     /locus_tag="P5293_00885"
     CDS             complement(17609..18361)
                     /locus_tag="P5293_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HRR48521.1"
                     /translation="MKKILIVLSLLLCVSQILMAQEEDQFRQKNLFKINLIPIAINNY
                     SIQYERVLKKSISALVAYRFMPEGNIPMKDNIIDLANVDDQDVIDIINGAKLSNFAIT
                     PEVRFYLGKKGFGRGFYISVFYRYSNFDGKNIVANFEDEDVSVTVNMSGNIKSHTAGF
                     VLGSQWALSKHLCLDFWFLGPHYGTGKGDFSGVTLQPMTLEEQETVRDKLNDLDLGPY
                     DKTVDVTSNKAAISVKGPFGGFRAGLSLGIKF"
     gene            18494..20122
                     /locus_tag="P5293_00890"
     CDS             18494..20122
                     /locus_tag="P5293_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRR48522.1"
                     /translation="MNSSKLLLLSAILFTSSFSEAKNLVSIVPAPASMTLQKGHFILS
                     PKSTIVTNCNYENIENIISQFNSHISDFYKLPPMSRNMGNTAVKGSIFLKIEENSTAS
                     PEGYKLTVSKDFIEICASNYAGIFYGMQSLFQMMPPEKRKISKIKIPCVEINDFPRFS
                     WRGLHLDVCRHFMPKEFIFKYLDYMAIHKLNTFHFHLTDDQGWRIEIKKYPLLTEIGS
                     KRSETLIGRYSKDNNNYDGIPHSGYFTQDDIREIVKYAADRYITVVPEIEMPGHALAA
                     LAAYPFLGCTGGPYKTATTWGIFDDVMCAGKDSTFKFLEGVLEEVIELFPSSYIHIGG
                     DECPKTKWQKCPLCQQRIKEEGLLNEHQLQSYFTQRIEKYINGKGRKIIGWDEILEGG
                     LAPNAAVMSWRGEEGGIAAANQQHYVVMAPTSYCYLDYYQGDRATEPIAIGGYVSLKK
                     VYEFEPVPASIKPENTKFILGGQGNVWTEYIATPEHVEYMAYPRAAALSEVLWSPKES
                     RDYTSFMNRMKIMIKRYDAIGLNYCRNEFIKPVK"
     gene            complement(20168..20587)
                     /locus_tag="P5293_00895"
     CDS             complement(20168..20587)
                     /locus_tag="P5293_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48523.1"
                     /translation="MKKLDIYIAIVVVLILLLILFTDPQNLLRIVIYLAIVFGLYFAA
                     YFKRLRERRKDWDISNRGLIFLICGHVFITMLFIASRGLDKTDDWVVSVGEFVLYISL
                     MVVFSMLVICKCVERHYYNESNKNSDEAIRSDEKDQK"
     gene            complement(20645..21877)
                     /locus_tag="P5293_00900"
     CDS             complement(20645..21877)
                     /locus_tag="P5293_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR48524.1"
                     /translation="MTFLISEQSTLKQIFSSLRSKNYRLYFYGQGVSLIGTWIQNIAL
                     SWLVYRLTGSVFLLGLIGFTTQIPTFILTPFTGVIIDRHDRLKIMRGAQVLFMIHAFV
                     MAFLVLSGLIEVWHIIALSIIFGVINAFDAPSRQSLVIDLIDDPSNLSNAIALNSAIF
                     NGARLVGPAIAGMVIALVGEGICFLINALSFVFVIIALYNIKIIRRKNVPSEDDFKKS
                     FMDGLRYTFGNKDIRNLILLLSTMSFSVFPAIVLLPAYAKGVLGGGADTLGFLMSALG
                     AGALVGALYMASRKSTKGLDKIISFSSLILALMIAITGVTKYFYLSLLTLAIAGVTMI
                     LTLSSINTLIQTSTEEKMRGRVMSFYAMALMGTQPIGNLILGAVASGIGVPVTILLGG
                     AVTFIGGIIWRYFSQNNL"
     gene            complement(22065..22241)
                     /locus_tag="P5293_00905"
     CDS             complement(22065..22241)
                     /locus_tag="P5293_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48525.1"
                     /translation="MRTADCVTYFQSKGKDFATFIDYHGKGNNMITVFDLKNEIAVQV
                     IGTIHSLPMVLLLR"
     gene            complement(22331..>23152)
                     /locus_tag="P5293_00910"
     CDS             complement(22331..>23152)
                     /locus_tag="P5293_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRR48526.1"
                     /translation="FANIDSLKLESNLIPHDFKIIKFGKTDLRRMNGEFEYVAYEQGI
                     PVRRFMVGPSNLSFRRLDQISPILQMAVLQSEDGGFFYHNGFLPEAIRGALVTNIKDK
                     RFRRGGSTISMQLVKNVFLSRNKTLARKVEEMLIVWLIENNRLTTKERMFEVYLNIIE
                     WGPMVYGASEASRFYFDKDVRDININEAIFLAGIIPSPKRSLNNFNADGTLISEKMDG
                     YFRLLAERLRVKGVISTSEEESVKPEIKLAGESFKIVRQREMEKDSSYVFQWRLK"
     assembly_gap    23153..23252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23253..24103)
                     /locus_tag="P5293_00915"
     CDS             complement(<23253..24103)
                     /locus_tag="P5293_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48527.1"
                     /translation="MLRDRKIIIGSVIIFLLISLLLLRGVIVRGFVSKKIENVERRYS
                     LIIDYEKLKINGFAGMSLNGLFVKPLDDDTLLMAKEINLKLNPFKLLILRFDIKSLIA
                     DNIQFSFVKRDSSSNYDFFYNVANRSVANGTETSKKLKQNYSRNLDNLLSMIFGFIPK
                     NAQIRNFLVDYRYDEYFLTVSTPEIKVANNFFSSTIITNECGHSEQLLLKGYLNDSER
                     KISAKIYSAGNKERFSVPFINFKWGAALQFNSLAFDFVSSQRKNGIVDIEGLTSIEGL
                     SLFHEKIS"
     gene            complement(24164..>24679)
                     /locus_tag="P5293_00920"
     CDS             complement(24164..>24679)
                     /locus_tag="P5293_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRR48528.1"
                     /translation="DKVIGCFGGGSNFSGISFPFLRHKLTEGRSIKLIAAEPAACPKL
                     TTGKLEYDFGDTAGLTPLIPMYTLGHDFQPEQIHAAGLRYHGGGRIVSQLKQDGFVES
                     CAIDQKEAFTAGILFARTEGIIPAPESTHAIAATIREALKCRESGKREVILFNLSGHG
                     FLDLGAYEQYV"
BASE COUNT         8226 a         4728 c         4165 g         7460 t
ORIGIN      
        1 cggatgataa ggttccattg agtccagctt tttttttgta ttagaaactg agtaacttct
       61 gtttgatttc aatagatatt gtctaaatcc aaatccgcaa caatgtcgtc tttcgggata
      121 atctacctgg gaacctcccc actcatctat taatcctgca agaacatatg gaaactcagc
      181 tccacctatt ccctctttag gaaatatttt tgcataatga cacccaatat gatcaacaac
      241 gtttaaaggt tgtccggttt tattgttaac caaacgatat ttcaacttcg aagatatctc
      301 tttacgaaac ttatatatca agtcactggc atgaactaaa tttttaggaa tctcaaaagt
      361 tcttccagtt gctctcaaaa gagcatccct tatttctctc tctttttccg ggaattcttt
      421 ccatgtttca agagtctctg tatataatcc aaacgatgtg acacaagaga ctacaaagtt
      481 ctcatatcca gcttcagtca taagcgaaaa ctgacgtgca ataactccca ttgttgtctc
      541 gaacgggatt attccggaat gatatgcaat cccagtacat gtagtttgac gagcatcatc
      601 ataaatatcc ttatgcaaat cattatgcat tatatcaaga aaaagaccct ctgcagcagg
      661 gaagaaattc tgtctgatgc agctccttgc aaaataatag ttatcttcag caatatgctt
      721 ctgatatgac tcccaacttt tacttttaat tattggttgc ataattacta cttttcatat
      781 aattccataa aatactcatc cttcccctca ctaaattcaa tccccatttt ctctgctgct
      841 ttctggctgt actcttcaat taaattcagc ctttcagtac ctcctgtctc atcaaaaata
      901 ttctttagat cactaagcga ttcttctgat gttagtctta aagttccttt cccggtcttg
      961 ttataatttg tgcttaattt ttcaagtact tcaaatctgt tttccttcaa ccaatcccaa
     1021 acaggtcctt gttcagggta catcccggta tcaacctcgt ctaaataaac acagtatcca
     1081 tatttgagaa tatgatctcc aacagttcgc ttgattgcca attgtttcct tccctgttca
     1141 ctacatacaa aaaaaccagt ctttatcgaa agcgctcgta atgcctggat aatgtacccc
     1201 ggagtattac cacgtgggca tcttgtcttg caagatagac actctccgca aaaccaaata
     1261 gtatcacttt taagcagttt ctctaattca ttttcattac ctcgttggac tatttctgtg
     1321 attattcttg gatcataatc gcggaattcg gctgcaggac atatggccgt gcaagtacca
     1381 caacttatgc acgccttaaa accttcgaca aaacgaaagt cttttttgag ttcattcaaa
     1441 agttccatct caataatcta tttagttata gcttttacaa aatcatcaaa tccactcttt
     1501 attgaagaac cattaaataa ttggtcaaga tcccccaatc acagtcagac aaagcaacca
     1561 atctttgaca aatcaaatat ttcaaaaggt ttgcacaaat tagccttatc gactatattt
     1621 tatcctatta gtatattatg aacatttgtt cgttacaaaa ataataaact ttcgttctat
     1681 ctcatactat ctattataaa aaacagaaaa aatgttcgta gtttttaaat tgagaggttg
     1741 tgattgtgac tttaccagag gcaacacccg tatttgaagc cgaacgcttg caaatggatt
     1801 tacagcaaat ttattcttga agatattgtc gatcgcacgt cccttgctgt ccatgctgaa
     1861 ttttaaaccc gggtacgaag aaaagtacgc tgaagactcg ggacgggaga attggctccc
     1921 ctggtctgta ttgattatct ccggggtgcc attccgactt atagcttatt caagacactc
     1981 gcggcaccag cggtcatcgt attggacagc gccatcctac gatgtagcgt aaaaactact
     2041 gtagctcaac aggttattgt gacggtgatt ccattcggtc acgtactgtt ggttccaggt
     2101 tttgtaatat tgctacgagt aaaaagtcgc aggaagttgg gtatcctgca atgaagccgg
     2161 agattcttat tttttcagat tattttgttc gctatcatga tttgtattgc tttgattgtt
     2221 gtctttgctt ttccttactt aatttgatta tcatcttaga ccgtcggttt tttgcttaat
     2281 ttccatgcca tatacacttc cgagacacca tagatcagca aaaatatgcc gatgaagatg
     2341 agaatggtct gggcgctggt gaaagggttg gtaaacagaa tgattcctgc aatgatcatc
     2401 agcatcgaaa tagcgaaata aatccacgac cagccgtagt cgcgtcggat gctcaatgtg
     2461 gcgatgagtt gaattatgcc gatcaacaga aatccgatgc ccatggcaat gacaaaaata
     2521 ttgaccattg ttgccggcaa tagtacaaac agtaatccga aaatgagatt gaatatggca
     2581 tctgccataa ggatgttttt agagcttttt tcttcactgc gaatagaaaa aacaagagaa
     2641 actattccgc taatcagaat cagtatcccg atgtagataa cgaaactccg taaggtaata
     2701 tccggcataa aaacagccaa tccgccaata atcaaggcaa taatacctct gagaagcgat
     2761 ccatgtgaag tggtaacaat ttgatgtttc ataaaattat tttttaaatg gttattgagg
     2821 tctatttcta caaagacatt gttcaaattt acattattat aaatcttaaa caataagtat
     2881 cattaaaaaa tagtattaat ttgtcgtcat gaagaaaagg aaaacttaca gtgcaggctt
     2941 taaagcgaag atagttttag aggtgcttta agaaagagaa acagtccagg agattgcgag
     3001 gaagtatgaa cttaacccga gtcatatctc tacatggaaa agtcaatttt tattcaacgc
     3061 ggatgttgtt ctcgaaaggg acacgcccaa gacagagggt gaaaaggaga aagatgctct
     3121 ctccaagaaa gttgggcagc ttcatattcc gttagggggc agtcacatgt acttaattgc
     3181 cataattaat gtttacagcc gctacatcgt aggatggtcg ctgtccaata cgatgaccgc
     3241 tggtaccgcg agtgtcttga ataagctata agtcggcgct tcgtacccgg gtttaaaatt
     3301 cagcatggac ggcaagagac gtgcaatcga caatatcttc atcgaaagat tctggcgaaa
     3361 catcaaatac gaaaagatct atctcgaacc gtctgacaga gagggagaag tttaatctta
     3421 attatcggac aatactcatt tcatcaataa ggtaccctgc accagcaaat ttatcaatga
     3481 cccatagtat ataacgaata tcaacattta cacatttatt gtattcttcc ttaaaataaa
     3541 catcaccagc cattgattct cgattgccat cgaaagcaag gccgattagt tcacctttac
     3601 cattcattat agggctgcca gagttgccac cggttatatc attgtcagta agaaaatcta
     3661 tataaagatt acctttctct ccccacatac cccaatctct ctgctcaagc attcccttga
     3721 ctttagaatc aagcttgaag tcatactcct taggattata tttttcaaga atgccattag
     3781 cttctgtctg ataatgataa tatacggcgt cagcaggtct taatgaacca acctcgccat
     3841 aagtaagtct catagttgaa tttgcatcag gatagacagc tatccccttt tccttctgca
     3901 tctcatacaa ggcatgcaca tacgctgttc tcaatgatgg catatccagt ccggcatcgt
     3961 ccagtatttt actttcagtc ctgttatact caatgatcct gctagtattt acaattatca
     4021 atatcggatc tgtttccatc tccttgatgc tgaccttctt tgagaaaaac tccttcaacc
     4081 tgtcgggatt agtgacaacg cttttgtcaa aaatataatc agctatcttt gaagcatcac
     4141 aaccgaatga gtctgcaagt tttataaaat ctttatcata aaaacttctt ggcacctcct
     4201 tcagataata acccaacata accttgaaga gttctttgtc tgcttcaata ttacattgag
     4261 aaaagatctt gtcacttgac tgcataaatg atatcaactc tttactgcca ggaacaatag
     4321 aatcctgttt tgctctggtc atactgttaa tcaatgaact gaatctttgt cctattccta
     4381 taatctcaga tcctcgaatc atcgattcac gaaaatattc agtgattttt actatatcag
     4441 aagtgagttc atatcctttt tttaaatctg aaagaacatt tccatatttt ttttgccgct
     4501 ccggattagc cgcaatccat tctgcaagtt ctttttctct ttcctcaaga acttgttgca
     4561 cattaaacct ttcaaggcat ttattctccc attttgcata gtcagtataa ttgctgatag
     4621 aaaaatattt gtctgcatac atgagtctga tagcaggatc cttttccata tatttctccc
     4681 aaacgtcaag tttagctctg cgaacagtgt atattgcagg attaagaatc tcctgttttt
     4741 gtttaagctg aaacgaggac atatacctgt ttgtagaacc aggataacca atgatcatcg
     4801 tataatcatc tggtttatag ccttttgcgg aaatttttaa ataccggttt gccttaatgg
     4861 gaacattctc ttcggagtat tctgcaggct taccgtcttt tgctgcatag acacggtaaa
     4921 tagcaaagtc tcctttatgc tgaggccagc cccaattatc agtctcacct ccgaatgcac
     4981 cgacagaaac aggaggtgct cccacaagtc tgatatccga ataagtttcg taaaaataca
     5041 gatagtactt gcttcctcgc cacatagatg ctaatgaaac ctcaaagtca gatttgtacc
     5101 tatcttctaa tactcctgat acttttctca taaaaaatat acctcttggc cctattttgt
     5161 caagagaatc tattattctg ttggtttcat ctgtaacatc aagtactttt ctaaggaagg
     5221 taactgtttt cccttttata ggtatctctt tatcacggct catggcccag aatccatttt
     5281 caagataatt attctcaggt gtgcttattg cttgaatatc accataagca cagtgatgat
     5341 tagtaataag aagaccttcg ggtgagataa tacttgcagt gcagcttcca ccatcaagtg
     5401 caactattgc atctgtaaga gcaccgccat tttcgttata aatctcatta ggagcaagtt
     5461 taagcccctt gtttttcatc ttagtggcca gctgcttatc cacagcatta attagccaca
     5521 tcccttcgtc agcttgtgca aactgctgta gtacacacaa tgacagaatg attagtaata
     5581 attttttttt catcgattgg gttaattaaa tttacgtaac tatcagttct tcacaaaagt
     5641 aattacaaaa gcaacttttg tttttaactt tttgcttaaa ataagtctaa tatgtaaata
     5701 tgttatttga tatacaaatt tagttaattt tgatacaaat ttagttagtt ttgtatattt
     5761 attacatgta aaatttatat taaccaaaaa aactcacagt tcatgaaaaa attcaagaaa
     5821 aattggcttc gccatttact tcaatgggga acccttcttg caattattgt ctttttgact
     5881 aaagtcttcg gaaatcaaac cgctgaccct gagtcatact gcccaatggg cggtctcgaa
     5941 tccttaagta catatttagt ctccgggtca ttagcatgca gtatgtcaat ggcccaaata
     6001 atgatgggta ttgtgctggc tcttactgta gtccttttca gtaagctctt ttgtggatat
     6061 ctctgtcctc tcggctgggc tacggagtat cttggaaaac tccgcgcaaa aatgaagatt
     6121 aaagagattg ttatcaaaaa cggttcagta cttgacagag tattgagact cgttaaatat
     6181 gctctcctct tcatcctctt ctattttacc attacaaact ctgaattatt ttgtaaaaat
     6241 cttgatcctt actatgcaat tgcgacaggc tttaaaggtg aaatcactct atggatgaca
     6301 attactgcaa tcgcactttt agttttggga agcttcttta tcaaaatgtt ttggtgtaaa
     6361 tatatttgcc cacttggcgc tataagcaac atttttaaat ttacaataac ttttgtagtg
     6421 cttgtaggta tttatgccat tctgggaatc agcggtcttt caatatcatg ggtttacctc
     6481 cttgctgcta catgtcttat aggttatata tgggaatctt tttatcttga gtcaaaggta
     6541 tttccacttc tcaaagttca ccgaaacaca gaaacttgca ataattgtgg attgtgtgca
     6601 aaaaaatgcc cttacagtat tgacgtagac aaagtagaga atgtgaaaca tgtcgattgt
     6661 acactttgcg gagagtgtgt ggcttcatgc gacaaaggtg cactgacttt cggaaaaaag
     6721 aaagggttaa ggtggcttcc ggcaatattg acggttattt tacttgctgc agcatttttc
     6781 ttgggtacaa tgtgggaact tccgacaatt gatgtcacat gggcagatca atcaaaaata
     6841 gaacaacttc aaaaaacaga aattgacggc ttgcgttctg tgaaatgtta cggaagttct
     6901 atggctttca aggctcaact tgagaaagtt ccgggcgtat acggagtagc tacatatgtt
     6961 aaaaaacacc aagctgttat tctctatgac ccttcagaaa ctaatccaga taaaataaaa
     7021 gaggcaattt atacacctgg taaatttaaa atagcaaccc ctcctgttgg agcacagatc
     7081 aaagttgtaa caatttacac tgagaagatg tatgacaaaa tggatccaaa ctatctcggg
     7141 atgcagttcc gtctaacaaa aaaaggttac tatggtttgg aaacagaata ttcttgccca
     7201 ctcacagtta gactatatat ggacgttaac gagcctgttg atgaatcctt catcaagtca
     7261 atagttgaga tgaaagagct tgcaatgcct gctcaaaatg gagaaacaaa tattataaag
     7321 gtcgattaca agtatgaagg aatgtctgat cagatagata caatttcaag acgggagttt
     7381 cttgaaagac agatgtataa atttgacaag actttcaaag ataattatga aaagtgggga
     7441 ggaaaggatg aggctgttta tgaaattgta tatgaaaatc tcgataagcc aattattacc
     7501 aggtacattc cgtaccttgc aagccatctg tcaatgatag atggattctt gggtttagca
     7561 gttgtcctta acgacaatga agattatgca ttccgaatta cttactcaaa agctgtgctt
     7621 gatgacgaca aaatctgggc tgctcttaca caacctaaat ggaaagttaa atcaaaagat
     7681 ggtgagatta gcgagactga tccaaaattt tcattcgaaa caaagggtca tactattgtt
     7741 atcaaaccaa atgatgtaaa atctaaataa tccaagaatt aaaaaagtaa ataatgaaaa
     7801 ataatataat tacaagattt cttgtaatac ttttcctgct ttctgtatcc ggaatttcaa
     7861 gagctgatga aggtatgtgg cttgtcaatc tgcttgacaa aaatttaact cagcaaatga
     7921 aaaaaaccgg ccttaaattg gatcctaaac taatttatga tgaaagcggt tcagcagtaa
     7981 gcaatgctgt tgttgcacta gattttggtt gtacaggaag tattatttcc gataagggac
     8041 ttttaataac caatcaccat tgtgcttatt cggatatcca tgcactcagc actcctgaaa
     8101 agaactatct cgaagatgga tactgggcta tgaatatcag tcaggaaatc cctataaaag
     8161 gcaaatcagt ctttttcttg agaaaagtca cagacgtaac ggatgagatt aaacaaattt
     8221 cagactcact caagaaaaac aacataccag ggggcatgag aaaaattaca aatatcatcg
     8281 aaacaaagca ttcgaaacag tctggtatgg agacatcatg caattcaatg tggagaggat
     8341 caaaatatta tatgttctac tacgaagtat ataccgatat ccgttttgta ggtgcaccac
     8401 cggtgtctgt agctgcattt ggcggtgaaa cagataactg ggaatggcct cagcataagg
     8461 gagattttgc tttgtatagg gtttatggga atagagatgg caaacctgct ccatattcag
     8521 ttgacaatgt gccgatctct cctaaaaaag tacttccaat ttccacaaag ggaataaagg
     8581 atggagactt taccatgatc ctgggttttc ctggaaggac aaacagatat agcagttcat
     8641 tcagcattca cgaaaaggaa aaagtaacaa atcctatatc agtgaaaatt atgagagaga
     8701 aactggatat tctcaacaag tggatgaaaa gtgatccaaa tattagacta aaatatgcag
     8761 attattattt tggaatatca aatttacagg aaatgctgga aggagaagtt atgaactttc
     8821 gccgcttcgg agtggaaaaa attttccaag aaagggagaa agaactccaa caatggattg
     8881 acagtgactc ccaaagacaa gcaaaatggg gaggccttct acagaaacta tctacaagat
     8941 ataatgggac agaagaaata acacgcatca aaaaatatta tcaaaacact ttggtaggag
     9001 gttatggcct tatagcaatt gcaaacaagg caggctcgct gaataattcg tcaaacagag
     9061 acaatcttga tacactcaaa gtcggacagc gcgaatatgt taatttcata aaagaaatag
     9121 agaagatcta taaagaatgt gatatgagag ctgaacacga aatgctcaag tatacactca
     9181 aaacatttgt agacaacgtg cctcctttat acaggggaga ctacattaac ggtcttgcaa
     9241 agcagtttaa taatgatggt gaagctatgg ccgattatat ttttaacaac tctattttca
     9301 cgaatcccga aaaattcaga caaatagtaa aaaatgatca gccggtttct gtttttggag
     9361 aagatcctct taatctgatg agtaaatcaa taggagtaag accatttaat gaagctattt
     9421 ctaaaatttg caaaggagaa agtatcgatc aactcgaagc agagtatgga agagtcgtgt
     9481 accaaatgaa tctcgataaa ggtagaaatc agtaccctga tgcaaattct actatgagac
     9541 tcacttatgg cacagtcgga ccacttgatc cgtcagacgg tatacactgt aaatcacaaa
     9601 caactactca ggggctatgg gacaagtaca atcctgataa ctatgatttt aatattaaac
     9661 ctcgcatgct tggttttctg gaaaataaag attggggaaa ttattgtgaa aaagggaaaa
     9721 tgtttatcaa ttttctcact gataatgaca taacaggtgg taattctgga agccctgtta
     9781 tgaactccaa tggtgaaata ataggactag catttgacgg taataaagaa tctcttgcat
     9841 cagatgcata ttttcatcct cagatgaata agtgtgtcaa tgttgatatc aggtatgttt
     9901 tatggataat agacaaatat gcaggtgccg gatatcttat agatgaaatg catattataa
     9961 aataaataga attaaaaaaa tatttcaaga tgacaatttt taataaaata ctttgtaccc
    10021 tttttatttg tatgggtata tcttctgcct acgcccaaaa tggagcaaat gacaagaagc
    10081 catttaacag aagtgaaaaa ctttcagttg atcctgaaat caaaataggc aaactcgaca
    10141 atggcttgac atattatatc agaagaagtt taaacccaac aggacaggca gaatttttta
    10201 taatccacaa tgttggttct ttacaagaaa atgatgacca aagaggtctt gcccattttt
    10261 tggaacacat ggctttcaat gggacaaaaa actttccgaa aaaaagtttg cttgaatatt
    10321 tcaatcggat tggagttcag tttggctaca atataaatgc atatacctcc atggacagaa
    10381 cggtctacaa tatttcggca gtccctacat atcggtccac aataatcgat actgcattgc
    10441 ttgctctgca cgattggtca cactatatat catgtgaacc ggcagagatt gaagctgaga
    10501 gaggagtaat acgtgaagag tggagacgtg gagacgatgc aagaagtaga atgatgaaag
    10561 gtatttcaaa gattgaacag acaggttctc gatttgctga aagagatgtt atcggtttga
    10621 tggaggttgt aaacaacttt acaccacaga ctcttgtcga ctattatcat aagtggtaca
    10681 gacccgactt acaagcagta gttgttgttg gggatattga cgtcaatgat attgagcaga
    10741 gaattataac attattttcc actattcctg cagcaaaaaa tcctgcaaaa agagaagttt
    10801 acacagttcc tgacaacaaa gaaccaataa ttggtttact tacagaccct gaaagcaaat
    10861 catattcttc aagattaatt attaaaatac caaatttaac tccggaagaa aaggccacta
    10921 ataaactaat ctacagagaa gtaatgaaaa cactaattgc agatatcatg aaagagcgta
    10981 cagctatagc ttctcaaagt aaagatgcac catttagaaa ttgtgtgcct gtattcagtc
    11041 aaatcaacta tgccaaaaca actttcatca tgactgcaat gcctcactca cctgctgata
    11101 tgttaaaagc tacaaaaggc atattgaatg aagttgaaag acttacatac tatggagcag
    11161 agcaagaaga gcttgagaaa gcagttcaaa atgctaaaat tacttttgca aaaaattatc
    11221 aaaagagcaa aaaacctaag aatatcgatt gggtaaacct aattgtcgaa aactttactc
    11281 gagactatcc actaataact cctgaaacat tttacaacat agctgttagt tgtcttgaca
    11341 atattacttt ggaagatttg aacagcagca taagaagcat gcttacaaaa gaaaatagag
    11401 ttattgtttt gtttgcacca gaaagtgaaa aacagtattt acccacaaaa gaagagattc
    11461 tcaaattgtg cgaagagatt aatggtaagg atttagcaag atttaaacct gtaagtaaaa
    11521 gaggccttaa ctttaatgta accttgagcc ccaaaaatat aacatcgatt aagcagctaa
    11581 ctaacaaaga ctttaaaact cctatttaca agcaacttga ctcaaccgtt gaaattatcc
    11641 tttccaatgg gactcgagtg atctggaatg aacacaaagg cagttcaaat tcagtacaga
    11701 tgagagcttt gagagaagga ggctacttca ctaacataga tgttatggac atcaaatttg
    11761 ctgacagata taatactctt ttctctgtcg attctttatc aaaagatgag cttgcaaaat
    11821 ggtctttcag cagaaacata tctctgagat tttccccaac aatgagatac agcacactat
    11881 ccggcgattt tgatcctaag aaaattgatg atttctttaa gctactttac acatcgcttt
    11941 cggagtgcac agtaaacaaa gatgatttta aaagatctat cagcaatgct atcaagaata
    12001 tcgagcaagg caaatcagaa actaatatct tcaatgattc agtctcaaaa ctcagattta
    12061 agacacgcaa aagaggagat gacttcacaa aagattattt gcagaatgtt acacctgaaa
    12121 aacttgtagc tcttcatgaa gcactttata agggaataaa tggttataca tttgtatttt
    12181 caggtccaat gagtgcacaa gaggcaaaac catatcttga aaaatacctt tcaaatgtgc
    12241 ctgaaaataa aaaattacct gctacaaacg tttcgcgtta tgacgagagg acaacaggcg
    12301 aggtttatct tagatataaa gctaaaaatc aaaagagtac taaagcatct gtaacaagat
    12361 actattactc cccaatggac tataatgcat taaacaacct taatgctaaa ttcttctcca
    12421 caattatgag tgacagatac atggcatcca ttagagaaga aagaggaggc acatatcatg
    12481 taggatgcag ttcatatctg gaaaaatatc catcacagat tgccgagtgt agtattgatt
    12541 ttaatacaga tcccaaacta gtagatgact tactacaagt tgttcaagat gagatggata
    12601 attttaaaaa gaatgggcct acagaacaag aagttacagc atacaaacta tatcttcaaa
    12661 agaaatttgc ttcaaaaaat ccggccgaat actcttggat aaccaccatt tctaatgcac
    12721 ttatgggttt ccctactttt gaagatcagg aagaagcact tctcgacaag attactgtta
    12781 aaagtgttaa gaaatttgca aacgatttaa ctaaagataa aaatagaatg acatttattt
    12841 ttgaacctat attataaaat aatatttaaa tctaattctg taaaataaga ggctgtctaa
    12901 aaaaagtaga cggcctctgt tgtcatctac tttcaagtat caagtaattt atttccagca
    12961 ctgtactatt atactgaaat agccaatcca gatattccaa ttcaattaat gtagcatttt
    13021 gaatactttc cacaaaacta tagtagtcga taactccctt ttcataacca tctcttgcct
    13081 ctttttcaat ctccctgctc atttgttttc ctgtagtgta atagtattcc aaatgacctc
    13141 tgtatttttg caattcactc ttcagctctt caatcttcag taaaagagac aatttgtaat
    13201 actctgcata tttcctggaa gcttcaattc ctatctttgc cgcattgatt cttgcctttt
    13261 gctcaataaa aaataatgga attgttatac caatttcata agaaggataa ttttttgaac
    13321 ccacaaaacg atttgagcca ttataataac ctatagtaat gtccggcaaa agtttgtttc
    13381 tttctaccct taacatatcc gactgcaaat ttacctcctg attaacatac tttagtcccg
    13441 gattagagtc aatatcctca tttaatatag taagctcctt caaatcactt agtggcacta
    13501 caataagagt atcagcatta attaaaagtc tcagcttttt ataggcaagc ctggtagcat
    13561 aagtaatatc attcaaaagt atctcaatct gctgatgttt agctcttgca ttaaaaagat
    13621 caagactctt ggcttctcct ctattaaatc tatcctcaac attttgtata aatttatcgt
    13681 aaatagtatc caatacacgg taacgcatta atttattcat gttatattga atctcatagt
    13741 atgagtgtgt aacatccttt attaattgct tttcaacaat ttccaactct ctattagcca
    13801 gctcactctc ggagcgggta actttgtatt gggcagaata aagagtcgga aaactaaaac
    13861 tctgttcgat cccataaaca tttaaaggat gattgttctc tgcaagatta ttcgaatcat
    13921 aagaatatga tatggtagcc ttgtcaatag taagagcggt cgggattaat gtcttgcttt
    13981 gctcgacctt aagtttgcag gcattgtatt caagattgtt caaaagagcc agttctattg
    14041 cccgttcaag cgaaacgccc ttagtttgac caaatacaaa attgatattt ataaaagaaa
    14101 aaacacttaa gaatcctatt aaaaatctat tcataaaaaa ttatttacta ttatttacta
    14161 cagtagttaa cattaagatt taatttatcg gtaataaaat cttctacaat cgatccaacg
    14221 aggatataat cgtagctcat gctcgagtgg tataagctct ttaatgtctg taatctgata
    14281 ataccactaa tttcatttag tccttattga tttgaaataa ttcatcgtta ccataacttg
    14341 tgcagttttt gcaaggtgta tgattttcaa taccctcgat taatttgttg cattttctgc
    14401 aacgatacca aattgttcca aaatggacat ttcctccttc aattataatc gtttctcctt
    14461 caacaaatgc ttttgcaatg gttttgcgtg cattttcata aatacgggta agtgtcggac
    14521 gcgatatgtt cattttttca gcagcttctt cttgaagtaa tccttcataa tccagtaatc
    14581 gaattgattc atattcgtcg taaagcagaa ttacttcttt aatcatcgag cgcggaatac
    14641 caaaaggttt aaagcctgac atcaaaggcg gagaaacaat ttttcgacat tgttttgggc
    14701 gagacataaa tttttctttt aaaataattg aaattaatgc agcaaagata taaatatttt
    14761 gaacatttgt tcataatctt gatgaaaaat ctaattttct tctgagatga acatattaaa
    14821 aaataataat attaaaatgt atcacattac cttttaaacc cttactgtga ccatgaaatt
    14881 gatatattgt aattcttatc atgtacttag attatttacc ttgtctccat ctactaaaaa
    14941 gcgactcgct tttaggaaaa atgccttttc ttttagaacg aacatcttcc acgataaaaa
    15001 aggcctgggg atcataacgg ttaattaaat tttccacctt cttcattgat ttcctatcaa
    15061 ttacggtttc tacaatatct acctgagcct gagatccttt tcctgaaata ctagtaccac
    15121 catatccttc gctactaagt atataaacaa gaggcgagcc tcctttctga gtaaaaatcc
    15181 taacgagaag aactccgtaa gctatttttt gctcaattat tatgcccaca aaattgcctg
    15241 aggcgtagcc ggctgcatag gcaacataag aaagcatcga atttgctctc gaaaaaattt
    15301 cagatatcac cacaatccaa ataaatacct caaaaaaacc gataagagga gctttatatt
    15361 tttctccctt cgaaacaaag ataatcctga gagtaccaag tgagacatct actattcgtc
    15421 caaaaaaaat aatgataggc aacagccatg ggtatacatc aagaaaatca aacatttttc
    15481 aattaattat gcacaaagtt aatatttaaa caaaaaataa ttacttttgc actccccaaa
    15541 gcccagatgg tggaattggt agacacgctg gtttcaggga ccagtggctg aaaagctgtg
    15601 caggttcgag tcctgttctg ggcacacagt agttttaagt tttgcttttc tgactttagc
    15661 tcgctcttca ggcgaaaggt tttctttata gtcataaata cccttctccc attccccata
    15721 gatagcattc ggaataacaa taaatcttct tccccactcc tgacgaaact tttctacagc
    15781 tcccgctctg gcgatattgt ctttaacctc aaaacaatca gcaaaatcat ttagattgtc
    15841 ccccagcaaa aggatgattt tataatctcc tgccacaacc ttacgtctcg attcttttga
    15901 ggaaccgtct gccaaaaaaa gcatgtgctc cttatctgca aaagggaagt tcagatacct
    15961 caaattaaag attgtggcgg gaatatctct gggagttcta ttgctaatgt aaaaaatctc
    16021 aactcccatt gaagctgcaa aacacaaaaa atcaacagat cccggtattg ctgtagctga
    16081 ggcttcatct atccatttag tccaatctgc aaaattataa gacttacctt cgacaatatt
    16141 tcttgcctct gaataactgt tattgagaat agtctcatct atatctgtta ctattgccaa
    16201 tctctctcca agcctattct ttgcaagctc catttcaaga gttgagcgtg cagcattaaa
    16261 tgcctgataa caaagtgcca tatattctgc agaaagctga tgccagagca cagggtataa
    16321 ttttaaatct tcctcactct gtaaattgtt agaaggcaac acctgagccg tagccgaata
    16381 aaagaaaaat attacgatgt ttatgaaaat aaaagctttt ctgtaaatca taataagtga
    16441 aatttatgcg aaagtagcaa ttttgcctgt ttatccataa aaagaataac tttgtcgctc
    16501 aaattagaat tgaaagttgt accatgaaca ttgaccacat tgaagctatt accggtaaaa
    16561 gtaaacttga agctcaggat ctaagatacc tcctcactct atctggcgat gatatgaaaa
    16621 ggctgtttga gatatcctcg tcagtgaaaa gcgaatatgt tggcaataag gtgtatttca
    16681 ggggattgat agagtattct aacagttgtg tcaaaaattg tttttactgc ggaataagaa
    16741 ccggaatgaa acaactgcac cgatacaaac tgacggatga tgaagttttg gaagctgcaa
    16801 agtttgcact gcaaaaaaga tatggaagta tagtcatcca gggaggtgaa cgttcggaca
    16861 atggtcatgt tgccaatatt acacgactac tacaaagaat aaacagagag accaacaatc
    16921 aacttcacat aactctctct ttaggggagc aaagtgaaga gacatatcgt atctggaaag
    16981 aaaatggagc acaacgctat ctactcagga ttgagacttc aaacgaagat ttatataaaa
    17041 aactgcaccc ggatgatgat ttacacagct tttcaaggag aattaaagca ctcgaagatc
    17101 ttaagaaact tggttatcag gtaggttctg gcgttatgat cgggctccct tttcaaacaa
    17161 ttgacgatct tgcccaagac ttattgtttt tcaaggcaat tgatatcgac atgaacggta
    17221 tgggacccta tattgaacat gaaggcaccc ctctttataa ctacaggcac ttactcatga
    17281 gtaaggagga gagacttgcc ttgagtctta aaatgatagc tgttctaaga attttaatga
    17341 aagatattaa tatagctgct acaactgcca tgcagacact tgctcctgat ggaagagaac
    17401 aggcattggc tgttggtgca aacgtcatta tgccaaatat tactccggtt aaatacagag
    17461 agaattatca actgtatgac aataaaccat gtataaatga agaagcagaa caatgtgttt
    17521 cctgtctgga aaacagaata aaatccattg gcaatgaaat agggtatgat gagtggggtg
    17581 actcaagaca ctacctcaaa aaaaaattct agaactttat accaagtgat aacccggcac
    17641 gaaatcctcc aaatggtcct ttaacactta tggcagcttt attagaagta acatccacag
    17701 tcttatcgta aggcccaaga tcaagatcat tcagtttatc tctaacagtt tcctgctcct
    17761 ctaaagtcat tggttgcaaa gtcacacctg aaaaatctcc ctttcctgtg ccataatgag
    17821 gacctaaaaa ccaaaaatca agacataaat gcttgctcaa tgcccattgt gaacccagca
    17881 caaaacctgc tgtatgagat ttaatattac cggacatgtt tacagtaaca gatacatctt
    17941 catcttcaaa attcgcaact atgtttttcc cgtcaaaatt ggagtaacga taaaaaacag
    18001 aaatataaaa gcctcttcca aagcctttct tcccaagata aaagcgaacc tcaggagtta
    18061 tagcaaaatt gcttagttta gctccgttaa ttatgtcaat tacatcctga tcatcaacat
    18121 tagcgagatc aataatatta tccttcatag gaatatttcc ttcaggcata aatctgtaag
    18181 caaccaatgc tgaaattgac tttttcaaga cacgttcata ttgtatagaa taattattta
    18241 tagcaatagg aataagattg attttaaaca gatttttctg tctgaactga tcctcttcct
    18301 gagccataag tatttgagaa acacaaagga gcaatgataa aactatcaga atctttttca
    18361 tgagtcaact ccttattaaa aatttaaaaa aacaatgtga atgtaatatg tttttctaaa
    18421 atgaaaataa aaaattgaaa gaataaaaaa gtttttttta aattggttcc gttaatacag
    18481 aaaagcaaaa attatgaatt catctaaact tcttctctta tcggctatac tatttacttc
    18541 ctctttttca gaagctaaga atctagtttc aattgtgccg gcacctgcct ctatgacatt
    18601 acaaaaagga cacttcattt tatcccctaa aagtacaatt gtaacaaact gtaattatga
    18661 aaatattgaa aatatcatat ctcagttcaa cagtcatatt tcagactttt acaaactgcc
    18721 acctatgtct cgtaatatgg gaaacacagc cgtaaaaggt tctattttcc ttaaaattga
    18781 agaaaattca acagccagtc cagaaggtta caaattgaca gttagcaaag attttattga
    18841 gatatgtgcc tcaaattatg ccggtatttt ttatggtatg cagtctttat ttcagatgat
    18901 gcctcctgaa aaaagaaaaa tatcaaaaat taagattcca tgcgtagaaa ttaatgattt
    18961 tcctcgattt tcctggagag gtctccatct tgacgtttgc cggcatttca tgccaaagga
    19021 attcatcttc aagtacctgg actatatggc aatacataag ttaaatactt ttcattttca
    19081 tctgactgac gaccaaggct ggagaataga gattaaaaaa taccctcttt tgactgaaat
    19141 tggttccaaa agaagcgaaa ctcttatcgg acgttattct aaagataata ataattacga
    19201 tggaattcca cactcaggat attttactca agatgatata agagaaatcg tcaaatatgc
    19261 agcagataga tatattacag ttgtccctga aattgaaatg ccaggtcatg ccctggcagc
    19321 acttgcagct tatccatttt tgggttgtac cggaggccca tataaaactg caaccacatg
    19381 ggggattttt gatgatgtaa tgtgtgcagg taaagatagt acttttaaat ttcttgaagg
    19441 tgtacttgaa gaggtcatag agcttttccc ttcaagttat attcatatag gtggcgatga
    19501 atgcccaaaa actaaatggc aaaaatgccc attatgtcag caacgcatca aagaggaagg
    19561 tcttttgaac gaacatcaac tgcaaagtta tttcacacag cgaattgaaa agtatataaa
    19621 cggcaaaggg agaaaaatta taggctggga cgaaattctt gaaggtggac tcgctccaaa
    19681 tgcggcagta atgtcatgga gaggagaaga gggtggcata gctgctgcta atcagcaaca
    19741 ttatgttgtc atggccccta caagttactg ctacctggat tattaccagg gagacagagc
    19801 tacagaacca attgcaattg gtggatatgt gtctcttaaa aaggtttatg agtttgagcc
    19861 tgtccctgct tctataaaac cggaaaacac caagttcatc ctcggtggtc aagggaatgt
    19921 ctggacagaa tatatagcca caccggaaca tgttgaatat atggcatatc ctcgagcagc
    19981 tgctttatca gaagtcctct ggtcaccaaa agaatccaga gactatacct catttatgaa
    20041 ccgcatgaaa atcatgatca aaagatatga cgctattggg ttgaattatt gccgtaacga
    20101 atttattaag ccagtaaaat gacttaagta atcaggtaag gtcccgcttt ctgttctttt
    20161 atagtaatta tttctgatct ttctcatcac ttcggatagc ttcatcacta ttcttgttgc
    20221 tttcattgta ataatgacgt tcaacgcact tgcaaatgac cagcattgaa aagactacca
    20281 tcaaagatat ataaaggacg aattctccga cagagactac ccagtcatca gttttgtcaa
    20341 gtccccttga tgcaataaaa agcattgtga tgaaaacatg accacatatt aaaaagatga
    20401 gtcctcgatt ggatatgtcc caatcttttc ttctttctct caacctttta aagtaagcag
    20461 caaaatatag accaaaaaca atagcaagat aaattacaat cctcagcaga ttctgcggat
    20521 ctgtgaataa tataagaagc agaatcagga caaccacaat ggcgatgtat atatccagtt
    20581 tcttcattat ttggatattc tatatccaaa tataataaaa ctaatgaata attcctataa
    20641 agatttaaag attattctga gaaaaatatc tccatattat tcctcctata aaggtaacag
    20701 ccccaccgag cagtattgta actggtacgc ctattccact tgcaacagct cctaaaatca
    20761 gattcccaat aggctgtgtt cccataagag ccatggcata aaagctcatt actctacctc
    20821 tcattttttc ttctgttgag gtttgtatca gtgtgttaat agaagaaagt gtaagaatca
    20881 tggtcacacc ggcgatagcc agtgttaaaa gagataaata aaaatatttt gtaacacctg
    20941 ttatggctat cattaaagct aaaataaggg atgaaaatga tataatctta tccaaacctt
    21001 tggtactttt tcttgaagcc atatataacg cacctacaag tgctccggcg ccaagcgcag
    21061 acatcaaaaa acccagagta tctgcaccac ctcctaaaac accttttgca taagccggaa
    21121 gtaaaactat tgcaggaaat acagaaaaac tcatggtact aagtaaaaga attaaatttc
    21181 ttatgtcctt attgccaaaa gtgtatctaa gaccatccat aaagctcttt ttaaaatcat
    21241 cctcagaagg cacatttttt cttctaataa ttttaatatt gtacagagca ataataacaa
    21301 aaacaaaact aagagcattt attaaaaaac aaattccttc acctactaaa gctatcacca
    21361 ttcctgcaat ggcaggaccc acaagtcttg ccccattaaa aattgcagaa tttagagcta
    21421 tagcattact cagatttgaa ggatcatcaa ttaaatcaat tacaagagac tggcgcgaag
    21481 gtgcatcaaa cgcatttatc actccaaaaa ttatacttaa cgctattata tgccaaactt
    21541 caataagccc tgacaaaaca agaaatgcca tgacaaatgc atgaatcata aatagcacct
    21601 gagcgcctct cattatttta agacgatcat gcctgtcaat aatgacgccg gtaaacggtg
    21661 taaggataaa tgtaggaatt tgtgttgtga atcctatcag acctaacaaa aaaacggaac
    21721 cggtaagtct ataaactagc caacttaaag caatattttg aatccatgtg ccaattagcg
    21781 agactccctg gccataaaaa taaagtctgt aattttttga tctgagtgaa gaaaatatct
    21841 gttttaatgt agattgttct gaaataagaa atgtcattag cttctttccc taaaaatcga
    21901 caccgcttat gcaaaaaatg tgctaaaaat attagaaggc ataatagctc aaagtattaa
    21961 tagcaacacc catatccaca ttttaaaaat taactgtatg atttgcatct tgtccaatga
    22021 aaagtgtttt catgacagtc tttccccctt ggaatcttca gtagtcatct caataacaaa
    22081 accatcggca gagagtgtat tgtcccgata acttgaacag cgatttcatt tttaagatca
    22141 aaaacagtta tcatgttatt ccccttgcca tgataatcaa taaaagtggc aaaatctttt
    22201 cccttactct gaaaataagt tacacaatca gccgtacgca tcagttcatt gttcttggaa
    22261 taaaaatcaa tcctgaaggt attgcatttg tcaaaagaat agctctcctc ccatttcagt
    22321 caggaaacct tcatttgagc ctccattgga aaacataact actatctttc tccatctccc
    22381 tttgtctgac aattttaaaa gactctcctg caagtttgat ctcaggctta acagactcct
    22441 cttcactcgt ggaaataaca ccctttactc tgagtctttc agcaagcagt ctgaagtaac
    22501 catccatctt ctcagaaatc aaagtaccat cggcattaaa attattcaga gatctcttag
    22561 gtgaaggaat gatccctgcg aggaaaattg cttcatttat gttaatatcc cgtacatctt
    22621 tatcgaaata aaaacgggac gcttcgcttg caccataaac cataggaccc cattctataa
    22681 tatttaaata tacctcaaac attctctctt tagttgtaag tctgttgttt tcaatcagcc
    22741 agacaattaa catctcctcc acttttcttg ccagagtttt atttctgctc aaaaaaacat
    22801 ttttgacaag ttgcatactg atagtacttc cccctcgtct aaatctttta tctttgatat
    22861 tagttacaag ggctccgcga attgcttccg gcagaaaacc attatgataa aagaagccac
    22921 catcttcaga ttgcaagact gccatttgaa gaattggaga aatttggtct aaccggcgaa
    22981 aactgagatt tgaaggcccc accatgaatc ttcttacagg tataccttgc tcgtatgcaa
    23041 catattcaaa ttcaccattc atacgcctca aatccgtctt accaaatttt attattttaa
    23101 aatcatgagg aataagatta gattccaatt tcaaactgtc aatatttgca aannnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaatttt ttcatgaaac agggagagcc
    23281 cttctataga tgtaagtccc tcaatatcta cgataccatt ctttctttga gaactcacaa
    23341 aatcaaaagc aagagaatta aactgcaagg ctgcacccca tttgaaatta ataaaaggaa
    23401 cagaaaatct ctctttgttg ccagcactat aaatctttgc agagattttt ctctcactgt
    23461 cgttaagata gccttttaat aacagctgtt cagagtgtcc acactcatta gtaataatag
    23521 tagacgaaaa aaagttgttt gccactttaa tttcaggtgt tgaaactgtt aaaaaatatt
    23581 catcataacg ataatccacc agaaaatttc ttatctgtgc atttttgggt ataaaaccaa
    23641 aaatcattga aagtagattg tctaaatttc tggaatagtt ttgtttgagt ttcttagatg
    23701 tttccgtacc attcgctacc gaacggttag caacgttata aaaaaagtca tagtttgaag
    23761 aagagtctct tttaacaaaa gagaattgaa tattatctgc aataaggctt tttatgtcaa
    23821 acctcaatat taataattta aaagggttta attttaaatt tatctcttta gccatcagga
    23881 gagtatcatc atccaaaggc tttacaaaaa gcccgttcaa tgacatcccg gcaaagccgt
    23941 ttattttaag tttttcataa tcaatgatta gagaatatct cctttcaaca ttctcaattt
    24001 tcttagaaac aaaacctctg acaataactc ctcttaaaag caaaaggctg atcagtaaaa
    24061 aaattatgac tgaaccaata attattttcc tatcccttag catgcaacaa ccctcgcata
    24121 attttggcaa agctactaat tattttagag atcagcctat cacttaaaca tactgctcat
    24181 aggctcccaa atcaagaaac ccatgtcctg acaggttgaa caagattact tctctctttc
    24241 cactctccct gcatttcaag gcttctctga tggtagcagc aattgcatga gttgactctg
    24301 gagcaggaat aattccctct gttcgggcaa ataatattcc tgcagtaaaa gcctcctttt
    24361 gatctatggc acaactctca acaaagccat cctgtttaag ttgactcact attctgccac
    24421 caccatgata acgaagacca gctgcatgaa tctgctccgg ttgaaaatca tgcccgagag
    24481 tatacattgg gatcaaggga gttaatccag ctgtgtcccc aaaatcatac tccagcttac
    24541 ctgtagtgag ttttgggcaa gctgcaggct ctgctgcaat taatttaata ctacgccctt
    24601 ctgtcaattt atgtcttaaa aacggaaacg aaatccctga gaaatttgac ccacctccaa
    24661 aacatccaat gactttgtc
//
LOCUS       DAOTQL010000008        24031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2159_length_24031_cov_5.380422, whole genome shotgun sequence.
ACCESSION   DAOTQL010000008 DAOTQL010000000
VERSION     DAOTQL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_24031_cov_5.380422"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..361)
                     /locus_tag="P5293_00925"
     CDS             complement(113..361)
                     /locus_tag="P5293_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48529.1"
                     /translation="MKISFTKRYEKDKDSGKQYPIMIANIEFSMEELERLKIADDRLS
                     RTISKSLLRLFNEMEFGTVENDPVEDVNINIARENDNL"
     gene            complement(393..641)
                     /locus_tag="P5293_00930"
     CDS             complement(393..641)
                     /locus_tag="P5293_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48530.1"
                     /translation="MLQRLLVNEIEKCEYKIKEALRELRDKVGLIPHSIEIELFWNSK
                     EEWRGAASRIPSEPFEDVARVHISLKKPNGARFPRDEK"
     gene            complement(1121..1330)
                     /locus_tag="P5293_00935"
     CDS             complement(1121..1330)
                     /locus_tag="P5293_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48531.1"
                     /translation="MFIVSENPRHKPVNPDLCAAIDKLIIPNNSMISFYITGGRIDWA
                     FSDDSECEKVYKWIIEKYGIPIYSP"
     gene            complement(1334..1504)
                     /locus_tag="P5293_00940"
     CDS             complement(1334..1504)
                     /locus_tag="P5293_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48532.1"
                     /translation="MFREKSIYDPNGYYNLKLKSTAKINIKKKTVEIYFGDVLRYHFE
                     VDKIVEINDKRE"
     gene            1635..1829
                     /locus_tag="P5293_00945"
     CDS             1635..1829
                     /locus_tag="P5293_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48533.1"
                     /translation="MINTLIAAVGGFLVALVILFFVWRNNKAKFTAILVILDELVKGA
                     VDEAKVKESVAKIKEILGVK"
     gene            1920..2429
                     /locus_tag="P5293_00950"
     CDS             1920..2429
                     /locus_tag="P5293_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HRR48534.1"
                     /translation="MNKQKRWDKYFLGLANSVAQNSQCLSRKIGAIIVLDKSIISTGY
                     NGPPRGVPPCDERWGKDDSLEFEFPERVRKGKCPRKAMGYESGEGLEYCIAGHGERNA
                     LINAARHGIAVKGATMYCNCPIPCTPCLVEIINAGIIEIVVTNLSHYDKTAEYLIKES
                     GIFVRKYEL"
     gene            2426..2638
                     /locus_tag="P5293_00955"
     CDS             2426..2638
                     /locus_tag="P5293_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48535.1"
                     /translation="MIELQIDKIKGGYTAFTRTRINRYALDEIRDRGGDIQDMIPEYN
                     KYSSISIIPDEESLDYFVELEESIIF"
     gene            2635..3009
                     /locus_tag="P5293_00960"
     CDS             2635..3009
                     /locus_tag="P5293_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48536.1"
                     /translation="MTVYTRSPKTRLDDWITGNGIVLCFHEGGTFWHEDGRAGSLYIH
                     TVPDNCPKENISILLLAHTCEKIKEAVEEYNSRPRDEYAGANLHYSRSLDDDILTENF
                     DRTLRRRKEDIQHIPDFLFKIK"
     gene            3020..3193
                     /locus_tag="P5293_00965"
     CDS             3020..3193
                     /locus_tag="P5293_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48537.1"
                     /translation="MRKRLKRKLRSCPSCKPHKTHGVPRWKNRDLHKLKLAEDEILET
                     KKKKPTEILEENN"
     gene            3243..3422
                     /locus_tag="P5293_00970"
     CDS             3243..3422
                     /locus_tag="P5293_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48538.1"
                     /translation="MKLQDNIKVNRRNHPMYGCRGKVVGIRGEIEQDNSWLLVYFPSH
                     QRSFLIPENYVLREE"
     gene            3522..4475
                     /locus_tag="P5293_00975"
     CDS             3522..4475
                     /locus_tag="P5293_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRR48539.1"
                     /translation="MSERVVWWNGKIIPEREARVSIYDSALMFGDTVFEMLRSFNRKH
                     FKLEEHINRLYNSAKYIRLEIPFTRSYLMNACEEISKLNEPNFNSDDEHRLMINVTRG
                     LLSIYRGIDLEEKGPNVIIADFPLRWTVQGMGKLFDVGVNAITPNQRTIPARYLEPKV
                     KNRSRLHYLMANIEVSLCSGENNWALLLDDDGFVCEGTGANFFIVERQGRLITPESRN
                     ILRGISRDYVRSLKSTVIRNIELYDVMTAQEAFFTGTPFCIMPCTSINGVKIGDGEVG
                     PVTKELLKIWSRNVEVDIIGQIKKWDEDNLKDGASPYSFKK"
     gene            4506..5441
                     /locus_tag="P5293_00980"
     CDS             4506..5441
                     /locus_tag="P5293_00980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR48540.1"
                     /translation="MKIFMDGGEGHIITHRMLPYWKKMGISIQLGNPQGCDVHLSYVH
                     LRAPSKLPKVLRLDSVYYDSDTNYNSRNAGISEAHRRADAVIYQSHFSRSMCEKYLSP
                     RKKEAKPFVIHNGIDNGWCGKFIEHEGTNIIVTAKWRRHKRLQEIIQLFLKHYQKYPY
                     THLHIFGLLYQNVPVKHPNIHYYGHVDRKDFMIHHSMADFTIHLSKKDACPNSVVEAI
                     GAGIPVITTNACGGSTEMCEMTEGCIICDGDEDSIEPCKPYTNAYNILPKILENNLLD
                     AMQKVENNKYRVKLPKELSAEYVAEKYFEVFKSIV"
     gene            5438..6346
                     /locus_tag="P5293_00985"
     CDS             5438..6346
                     /locus_tag="P5293_00985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRR48541.1"
                     /translation="MNNILKTSDGKEPLVHISKLPVVDIAVTTFNTERFVKDCVESII
                     KQTYPLWHIYFVDDCSSDKTLETIKSIVKSNNIKDQCSFFQTEKNSGYGTTLKWAIEM
                     GENELVAIVDSDDFLDNDDALKLVVEEHKKYPEVSLIYTDYNQIVSTHYTRKELIRAM
                     PPTNGKFLGKFKDGVYMGTDADISHLKVFKRGFYNMTIGLDHRLIKAVDKDLILKLEE
                     VGRMHYIRKALYCYRKHSTSITSVYRDRSIEYHKRVMDLKNAMYLNAKHRRERGILLR
                     VGDTYTYSENGKKMGLNCRGGSDVYC"
     gene            6333..7004
                     /locus_tag="P5293_00990"
     CDS             6333..7004
                     /locus_tag="P5293_00990"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRR48542.1"
                     /translation="MYIVSLIPAREGSKGIPKKNLALLNGKPLIYYVIQSSLNSICHD
                     TFVSSDGPDILGYAMELGTKVIEQPPIISGDRNSIEEVITYFTERIEYDILVLLAPTS
                     PLIESKHINDGVEKILVHGYDSAIGAMGMEKNDILFWDGIVRYPVNYELKKRGVRQDR
                     EENRYYIETGGLYVTTKKQFLQSKCRVGGNVGFVEIPFWNHFEIDTPEDLKMIECIMR
                     GKDSA"
     gene            6997..7527
                     /locus_tag="P5293_00995"
     CDS             6997..7527
                     /locus_tag="P5293_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR48543.1"
                     /translation="MRESKIGFLRTYIPDSFYGKNKMWLDCASGDGVIVARDLLKERG
                     IIPSNYICIDRDSEKLESVKKMGCKAVCIDLETSNIKEHVPMVDIILCLETLEHLEAL
                     TAERLVKDFISILVKGGMMIITFPINIVLEEKEGKCFNKHQPNIKNFLKLKEFFSGFI
                     KTSWNKSRVLVFMDKK"
     gene            7524..8315
                     /locus_tag="P5293_01000"
     CDS             7524..8315
                     /locus_tag="P5293_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HRR48544.1"
                     /translation="MNSLTQVNIELTSRCDKNCWMCGRRERERLYGKQGYGDMDFDMV
                     GLIADEVPTGIMVAFHNNGEPLLYPRLGEAISLFRDQTTYFVSNGLTLMKRANELIDN
                     LDIISVSVIENEREEVAERQYNEIIDFLALKGDRNPHFILRFVGKANEERYKDLDVLK
                     VRRVLHLPKGSMGYRKSPTIPEHGVCLDLLNRLAIDRFGNVSLCVRFDPEGELRLGNI
                     AETSLEDLWKCEKRKRIVEMHISGQRHKVPYCGNKCEFYGVPTGY"
     gene            8317..8838
                     /locus_tag="P5293_01005"
     CDS             8317..8838
                     /locus_tag="P5293_01005"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR48545.1"
                     /translation="MKEGHLDINQFLFPREKAFTDLHLLFCERNLRTILDVGGPSRAF
                     NIFNIQNWIYRYSNITGSQKSHFIQDDICNSKIESDSFDLVCSNDMFEHILEPWLAAE
                     HCVRICKSGGLLFHITLFYWDLHNSPIDCYRYSHIGLTHLFERTGKVKTINCGYYQHK
                     KSKQRVIYFGEKI"
     gene            8835..9359
                     /locus_tag="P5293_01010"
     CDS             8835..9359
                     /locus_tag="P5293_01010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HRR48546.1"
                     /translation="MSQFVAGEYTYGKIGIFGESEHNIYTGKFCSIATNVVAFMGVDH
                     RTDVISTYPLNIKWGRKDIKSHPISKGDIRIGNDVWIGKNSILFHGVSIGDGAVIGAC
                     SVVRRDVPPYCIFIGNPAQFIKKRFNDEKIEILLELKWWDWKQNIIQEAIDILMSDNI
                     DKLVKFAREKKLYV"
     gene            9352..9924
                     /locus_tag="P5293_01015"
     CDS             9352..9924
                     /locus_tag="P5293_01015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR48547.1"
                     /translation="MSDKGEENMNFKQTRKKWAGMEYVSLFKELIRLLEPHTYMELGV
                     SKGFTFNQISPLVKRAIAVDINDCRPHIILRENVEIFKMKTDNMALMWRDTIDFLFID
                     ANHKKEQVLKDFNNFTPFVREGTGIIALHDTHPTFPELIDEKYCSNAYEAAWEIRTNT
                     IYADFEVLTIPGPYAGLTLIRKSKRQLSWK"
     gene            9993..10637
                     /locus_tag="P5293_01020"
     CDS             9993..10637
                     /locus_tag="P5293_01020"
                     /inference="COORDINATES: protein motif:HMM:NF022760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR48548.1"
                     /translation="MDEKNTRMDLLFSYLEKNSIPSIASQTNKNFEWVWLIDDHSPDG
                     VKERVKKLSNILKSTTIFDKWESKVPFGEGNSCGGNWLRQFRENFRPGGIVSITRYDI
                     DDYISSNFIDVIQKHFEYREFCIDFENRISVDWKGEKYLTYNKPPSPIITLLEKTDKI
                     KTPYYYPHYKLDSYYQMKILPDIVYAKTEKEPHPSIIQSKLKRTNIKNFEALGE"
     gene            10642..11952
                     /locus_tag="P5293_01025"
     CDS             10642..11952
                     /locus_tag="P5293_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48549.1"
                     /translation="MLNVDFRMWKDFKKLSNNDNWNHMSFERNSEITYPWLENIKIPL
                     DDNIVDNLYASCVFSFMYPIHFPALISEIYRIMKPGGLIRTCVPDYEIGMKCYFDNPN
                     ILFKKRPPVPDFYPQTRLGRLMAWGSAHSSPEVYNEYVQRRISFDFETMEWYLKNAGF
                     INIQKRNHNDCSPIFEGRDFEVNKHFVLYVEALKPDNPIDICIMAVRRPELLDTLLKS
                     FKEKMNLNNNRIIINVDPVGENIPSERVIDIVYKYFNEVTYRLSDEANFTKAIKWCWS
                     NVKTEYFLSLEDDWELLTEINLPSMMKIMKDNKNLAQLSLRWEKWKKRYERKAEYFAQ
                     PMFIGHPSLMRKSYIEYVLPLLVDNKNVEVQLKAINTLFEFGQYTGSEEFVGNLRDHG
                     REWRESHGFIKTGPEKSDFILWKERAIEKSIDSNYDNSNNSSSE"
     gene            11921..12586
                     /locus_tag="P5293_01030"
     CDS             11921..12586
                     /locus_tag="P5293_01030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR48550.1"
                     /translation="MITVIIPVLNNINYTDDLFLNIKENTISPSEIVLIDNGSKDNYK
                     DLIKKYNDLNIKYVRNETNIGVNGAWNMGIEMSNYPLLTILNNDIIINRFFFQKIIKT
                     MEDVSIGLCVPNTIKNKNFVDENVLPKIANLERREGWAFTIRKEIVTKIGYIPDSLKT
                     FCGDDYLFMASKKAGFKNVKILNSNIFHVISATLKYDQAWKDRLKQEKSIWNRIWKEM
                     KDD"
     gene            12579..13298
                     /locus_tag="P5293_01035"
     CDS             12579..13298
                     /locus_tag="P5293_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48551.1"
                     /translation="MIDIFCTAIRRPIIVEETFKSFKENLFKNHKCRLIINVDPVGEN
                     ISSYEIVEIGKKYFDEIYPNTPTEASFSRAFKWVMSQGKNEYVFNLEDDWLLLRECDL
                     SDMINILERNKNLACIRLPNADFSCKWPANIPKIFLEDGKISLDHRFCGHPALWKMDF
                     FRDALKIYDENINPERQFRFDYEENKLEMKNLFKKWQLGVFYDKLYIKDIGRRYGGRK
                     NRAPKIYEYTEKYKYLVKWEI"
     gene            13309..14079
                     /locus_tag="P5293_01040"
     CDS             13309..14079
                     /locus_tag="P5293_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48552.1"
                     /translation="MFYKISELKRNTNIGIILGTAKSVNNISQTEWDEINKCDNLGIN
                     NWFYHPTFIPKMCSLELKSYDWQISKDRIAEKWDKGWKNVKWIISEKKHEYISGAIGH
                     ENEAQIYLYDFEIRDQKLHPKFVKNHKYNADFNPDVGPLVKSYDASLTTLVHLMYRLG
                     YKYIILYGVDWTHSYYFWSSGDEIYGKTHCKTNKDHEGKDPKLPHNTEHVIDYIIDFN
                     ERHMKPQGREILVGYKETRLYPHLNYIDVVNKEIHSRK"
     gene            14261..15100
                     /locus_tag="P5293_01045"
     CDS             14261..15100
                     /locus_tag="P5293_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRR48553.1"
                     /translation="MGKIILVAECGINHNGDLELAKRLIKKSKDVGMDYVKFQKRTID
                     LVYSKEELESYRESPWGTTFRQQKEGLEFNKEQYDEINRYCKEIGIPWFASPWDLESI
                     NFLTQYNPPYIKVPSALVTYRKYLEAIAETKIPVIMSTGMSNKNQIDTAMDILGENLE
                     ILLHTTATYPCKFSDLNLLKIRTLIKEYGHYYTIGYSNHSPSAIPILFAMALGAEVIE
                     THITHDRSAYGSDQSSALEKRQLETICEWRNELKETLGNGKWKIFDSEIPVMKKLRKH
                     YDF"
     gene            15090..15266
                     /locus_tag="P5293_01050"
     CDS             15090..15266
                     /locus_tag="P5293_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48554.1"
                     /translation="MTFRKQIGDKVVISNIFHEHYREVGLIVDIRGKWAMVLLDNQGK
                     IIVILLSDISKRFT"
     gene            15293..15502
                     /locus_tag="P5293_01055"
     CDS             15293..15502
                     /locus_tag="P5293_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48555.1"
                     /translation="MRENIKNFTKYLSDLEKILSKGDYESILHVFSLIVQARLAKIRN
                     QIEDAEIIPEQIEKPKKKKGVDIYV"
     gene            15495..15668
                     /locus_tag="P5293_01060"
     CDS             15495..15668
                     /locus_tag="P5293_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48556.1"
                     /translation="MFDDLFDDLIQKKKAPEVKQPEPEQDNPFGEYFDTGMGDTHDDV
                     WSSGQLPDYWKTK"
     gene            complement(15739..16395)
                     /locus_tag="P5293_01065"
     CDS             complement(15739..16395)
                     /locus_tag="P5293_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48557.1"
                     /translation="MLKILRQDKNAKILVATPLLTGHKIFKQTKVSLERSNVPIIWAS
                     YEGPGKHAENCQAGLDEYKKQFKSFPPYIQILDRDIVLGRNFLDKMLSTIEKTDGNIG
                     FVYSPFEYKGYINVIFPPKVYDINFLMNQNYISSNSLYKSDVIEKVGGFVTDKRIHRL
                     SDWAMWLKMYKFGYIGQLCSNAFFTAISSPSDISAGGQEEYLTTKKLVIEEFVKPLVT
                     "
     gene            complement(16402..16779)
                     /locus_tag="P5293_01070"
     CDS             complement(16402..16779)
                     /locus_tag="P5293_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48558.1"
                     /translation="MNSPKQPISRKQFRKAVMPVTGTQIGSYKIDYVNYAQLRFTAKA
                     SKIPDLGEIIEWMGRKYIVNHIIKDDRFSAQFCGFVENPVVGEGDKQLTDLPDVGDDL
                     TAAGPHITDDTVEATMFPEKVSE"
     gene            complement(16772..17080)
                     /locus_tag="P5293_01075"
     CDS             complement(16772..17080)
                     /locus_tag="P5293_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48559.1"
                     /translation="MSRKKVDITITKAATLNTGNFSSVKPEVSLTLKDVNIDDLMIEH
                     QKLSGLADALFALNTISLLDETNTYNQVGMRHYLEALKDSAPQMEAIIRNYSSGEEDE
                     "
     gene            complement(17155..18036)
                     /locus_tag="P5293_01080"
     CDS             complement(17155..18036)
                     /locus_tag="P5293_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48560.1"
                     /translation="MRNLFLLNVDKSLSKELSKSFNVIIPESEKNLPDNIDAVYIEYI
                     PYNSNNPQPFVLQTETFKFYLKKKIPVVVFDKYLAIKPDEARWLKKQNVKLLEPAINY
                     RPDFRYLPQFCPKIHSIDTLKLNKVEPRAVTIGFKGIIFDKLNSFEKYLVEFGKNYPS
                     FSTKYSDSSNISKEKTEEYTNLNVILDKNLKYSDIKCTVLLGSVRDYTIGYLNPDLFT
                     ILENNVIILLPEEHRYYHALFSDTVIKELNDMVFVAQTYDFVYLGYIANVYHRIDKLY
                     PEFKLEHTVDFIKKEIE"
     gene            complement(18173..19537)
                     /locus_tag="P5293_01085"
     CDS             complement(18173..19537)
                     /locus_tag="P5293_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HRR48561.1"
                     /translation="MAKYNIPLKTNCVPKTYEGASAKVLTPEQILRRSTMSCLLWESQ
                     FYENGENIADRILATIPQVNGKVVANLAVEAREKMNLRHVPLLLATGMARHIDHRKYV
                     AKTLERIIQRADELSEFLSLYWKDGKVPLASQVKKGLARAFTKFDEYQLAKYNRNNQI
                     KLRDVLFLSHAKPLNEEQALLWKKLINNELAVPKTWETELSAGKDKKTVWETLIMENR
                     LGSLALLRNLRNMESAKVDRNIILRGLDRMNTKKVLPFRFIAAAKYAPFFKKKLEELM
                     FKSIENLEMLDGKTIILVDVSGSMDMALSNKSDMTRIDAANGIALIAREICENVSIYT
                     FSNKTVMVSNSVRGFSLAESIHRSQPHDGTYLGSAIKHIISSEKFDRLIVITDEQSHD
                     NIPVNLPEALYYLINVASYRNGVGYGDWVHIDGFSEAVISYIIEYEKEFVKNIPNDIV
                     YLEL"
     gene            complement(19653..19874)
                     /locus_tag="P5293_01090"
     CDS             complement(19653..19874)
                     /locus_tag="P5293_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48562.1"
                     /translation="MFPTLLGAINIMLAPVADGVANSDSLPAQDAICTVLGGVMAHPK
                     LWGTTDTRNGLPTTTKESPNFSHGECQKI"
     gene            complement(20300..20626)
                     /locus_tag="P5293_01095"
     CDS             complement(20300..20626)
                     /locus_tag="P5293_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48563.1"
                     /translation="MSEKKEMGVFRIEYELLHNMSNWTAFIVAWSHEEALATLMKKVG
                     PNHRVLVSGFASRVDAISDEVRYWVVEKTIGKKKSEEKEEAMLQKAEESQEIEEKVKK
                     SSLKRK"
     gene            complement(20632..20832)
                     /locus_tag="P5293_01100"
     CDS             complement(20632..20832)
                     /locus_tag="P5293_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48564.1"
                     /translation="MKEKEFMELPIGPKDYVVGECFLEGKSIVVQRDTKKVGDLITYF
                     KIIEIKGDKRIEYVPVIERLEE"
     gene            complement(20991..21380)
                     /gene="tnpA"
                     /locus_tag="P5293_01105"
     CDS             complement(20991..21380)
                     /gene="tnpA"
                     /locus_tag="P5293_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRR48565.1"
                     /translation="MEYLTKNHSKYLLQYHIIFVVKYRLKIIDQFPIKDICSQLSIND
                     NYIIKVMEVDTDHIHLLVQAEPKISPLYIVKKLKGKSTIILWKNYSQILTRYFWKEHT
                     FWSDGYFVSTIGNVSEQTLQKYINEQG"
     gene            complement(21368..22789)
                     /gene="iscB"
                     /locus_tag="P5293_01110"
     CDS             complement(21368..22789)
                     /gene="iscB"
                     /locus_tag="P5293_01110"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HRR48566.1"
                     /translation="MKLEGNWLIRGLNLEGIMQTLQREFKNVPTDASLVCSSTSRALN
                     REETLSAHGKVLICNNPEVNQPQQGGDSNLRVSNIVFVLSMSGKTLMPCKPQKAKKLL
                     KGGKANVVKRFPFTIRLTMGTGETVQDINLGIDSGYKHVGFSSITEKEELFSGTLNLD
                     LKTKDRLNEKKMYRRNRRNKLRYRKSRFNNRKRKDNWLPPSIERKYQTHLTLIEKIKN
                     LLPIKNVIVEVAKFDIQKIMNPKINGKEYQQGNLFNYQNMVSYLQVRQNNICPYCKKE
                     FKGEPKATHHIYRHGDSRRSNRPDGLLLLHKSCHVDLHEKHREKEFQKPVKRYEPSTF
                     MSIIHKRFYEDITGLQVTYGYITQMKHNEYNIEKTHFNDAFIIAGGTQQVRCKPIIIE
                     QRHRNNRVLQLNRKGFKPSIKRERSKILPKDLFWSNNIKYTCKGMFNKGKYVLFGDSK
                     KKEYIKFTLIDKIYNFGSFVWNI"
     gene            complement(22807..23931)
                     /locus_tag="P5293_01115"
     CDS             complement(22807..23931)
                     /locus_tag="P5293_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48567.1"
                     /translation="MSNKSFFEDLQKRIAKNVKGVHASIMSQSEIASVSDWVKTPSLD
                     LNRILSGDINKGIPNRSLVGIVGPEHTMKSSFMVLCMAEAQKKGYSPIIIDTERGTDK
                     DFCIRWGLDVENTQYIYTPWENEIRSILAQIKGTGQKRMIIGIDSMGGVDRYKMYEDA
                     LDGDPKADQGLLQKNIRALLKLLLNICIEQESIGIVTGHLYGLPGVIPMPDQVGGGKA
                     MRLFPSILINLKKTPIKVDKKITGNEITAVTIKNRMYPPFQQATVSIDYVNGINPYAG
                     LLTHLITAGLVKEDGKTYTVINGKKEEKLGIGWDKSEANLSKYPEVLLKFNEWLKQTG
                     YSTINKETEAAIGELEDIAEEEISKINNNKKKKHKSITPE"
BASE COUNT         7946 a         3984 c         4488 g         7613 t
ORIGIN      
        1 caattcatct cccacctaaa gaggtgggag tattcttggt gatgttagat aaatggggat
       61 atacgcctca ccctcttttt ccaactccga aacttccact cttccaccac atttacagat
      121 tatcattttc cctcgctata ttaatattaa catcctctac aggatcattt tcaacggttc
      181 caaattccat ttcattgaag agccggagaa gagacttcga aattgttctc gatagtctat
      241 catccgcaat cttcaatctt tccagttctt ccattgaaaa ttctatattc gctatcataa
      301 tcggatactg ttttcctgag tctttatcct tctcatacct ttttgtaaat gatattttca
      361 tttttcattc ctttacgctc atatggggtt ttttattttt catctcttgg aaatctcgcc
      421 ccatttggtt ttttcaaact gatatgaacc ctcgccacat cttcgaacgg ttcactcggt
      481 atccttgacg ccgcccctcg ccattcctct tttgagttcc aaaatagttc aatttcaata
      541 ctgtgtggga ttaatcccac cttgtctctc aattctctca atgcctcttt tattttatac
      601 tcacattttt ctatttcatt gactaatagt ctttgtagca tatctcctca catccgtact
      661 atcccgaata taataagggc aaggataata gcaaagataa tataataaat catcaatgaa
      721 cctgacagaa ttgtatatgt ggtacacgaa atgatttgtc taaatatctt taccattgag
      781 acaccttgcg caaatatatt tgggaatttt ttgacttgaa atttgatttt catcagctga
      841 agtgtagcgg tggccgatta ttttgcagat tagtttcatc atcacctttc ttgggtattc
      901 caaattcatt cagggggcgg aaaaacactc gattacattt cagacaaacc acaagatcgc
      961 cgatggaaat gaatttcttt tctttgctac ccgattccgc cattccggca acaggaaaat
     1021 ctctccaata cgacctatga aacaaaaaac ataacatttt cttcatatta taccacacct
     1081 ttcttgaaag gtaaacatca ttatttgttt aaaacacaaa ttacggagaa tatataggta
     1141 ttccatactt ttcgatgatc catttataga ccttttcaca ctcactatca tctgaaaatg
     1201 cccaatctat cctgccaccc gtaatataaa aagaaatcat actgttattg ggaataatca
     1261 atttatctat tgctgcacac agatcggggt tgaccggctt gtgtcttgga ttttctgata
     1321 caatgaacat attctactcc cttttatcat ttatttccac tattttatcc acttcaaagt
     1381 gatatcgcaa aacatccccg aaatagattt ccactgtttt ctttttgatg tttattttgg
     1441 cagtggattt caacttcaga ttataataac catttggatc gtatatggat ttttctctaa
     1501 acatatcttt tctcctatta tacactaaat agacaaagat gtaaataaca atattattta
     1561 tcataaatag tacagggaaa ggggaattac aaaagtgtaa ttcccgttta aaaaaatacg
     1621 gaggtgcaat acttatgata aatactttga ttgcagcagt aggtggattt ctggtcgcac
     1681 ttgtaattct cttttttgta tggagaaaca ataaggcgaa attcaccgca attctggtaa
     1741 ttctcgacga gcttgttaaa ggtgctgtag atgaggccaa ggtaaaagag tctgtagcca
     1801 agattaaaga gattcttggc gtaaagtagg cttttcagtt ctgggggatg aattttcttc
     1861 atcccccatc tttacttcac attttttata tggtataatc tgtgtagagg aagtatttta
     1921 tgaacaaaca aaaaagatgg gataagtatt ttttaggtct tgcaaattct gtggcacaaa
     1981 atagccaatg tctttcaaga aaaatcggcg caattatcgt tctggacaaa tcaatcatat
     2041 ctacaggcta taacgggccg ccccgtggtg ttcccccttg tgatgagaga tggggtaaag
     2101 atgacagttt ggagtttgag tttccagaaa gagtccgaaa agggaagtgc ccgagaaaag
     2161 cgatgggcta tgaatcgggt gaaggacttg agtattgtat cgcgggtcac ggagagagaa
     2221 atgcccttat caatgcagca cgtcacggta ttgccgtgaa gggggcaaca atgtattgta
     2281 attgccctat accctgtacg ccctgtctgg ttgaaattat caatgcaggt atcattgaaa
     2341 tagtggttac aaacctttca cattatgata aaactgcaga ataccttata aaagaaagtg
     2401 gtatatttgt gaggaaatat gaattatgat agaacttcag attgacaaaa taaaaggtgg
     2461 gtatacagca tttaccagaa caagaattaa taggtatgct cttgatgaga taagagatcg
     2521 tggtggggat attcaggata tgataccgga gtataacaag tactcatcaa tatctattat
     2581 tcctgatgag gaaagtttgg attactttgt agaattggaa gaaagtatta tattttgaca
     2641 gtatatacga gaagccccaa gacaagactt gatgattgga ttaccggaaa tgggattgtt
     2701 ctttgtttcc atgaaggggg tactttttgg cacgaagatg gaagagctgg aagtctctac
     2761 atacacacgg ttcctgataa ttgtcccaaa gaaaatatat ctatattact actggcccat
     2821 acttgtgaaa aaataaaaga agctgtagaa gaatataatt cacggccccg tgacgaatat
     2881 gccggtgcaa atttgcatta ttcgagaagt ttagatgatg atattctgac ggaaaatttt
     2941 gacagaacgc tgagaaggag aaaagaagat atccaacaca ttccagattt tctattcaaa
     3001 ataaaataag gaggcaaatt tgagaaagag attaaaaagg aagttgagga gctgtccgag
     3061 ttgtaaacca cataaaacac acggcgttcc aagatggaaa aacagagacc ttcataaact
     3121 caaattggcg gaggatgaaa ttttagaaac aaaaaagaaa aaaccaacag aaattttgga
     3181 ggaaaacaat tgagggggaa tttttcccct cttttttttg tataaataat atcatggaaa
     3241 gaatgaaatt acaggataat ataaaagtaa acagaagaaa ccatccgatg tatggctgta
     3301 gaggaaaagt tgtgggaata aggggtgaaa tagaacagga caattcttgg ctcttggtct
     3361 attttccttc ccaccaaaga agttttctaa ttccagaaaa ttatgtcttg agagaagaat
     3421 gaaagaagtg ccaaaaatct aaagatagat ggtatgtgca taaatgcact ctggaaatgg
     3481 aaaaacatta agtgaatcat tttaaagaaa aggagtaaac tatgtcagaa agagtagtgt
     3541 ggtggaatgg taaaataata ccggagaggg aggctcgtgt tagtatctac gatagtgctc
     3601 tgatgtttgg tgacactgta tttgagatgc taagatcatt caatagaaaa catttcaaac
     3661 ttgaagagca catcaatagg ttatataact cggcaaagta catccgtctt gaaattccat
     3721 tcacgaggtc atacttgatg aatgcttgtg aagagatatc gaaactgaat gaaccgaatt
     3781 tcaactccga tgatgagcac cgcttgatga taaatgtaac aagaggactt ctttccatct
     3841 atcggggaat tgaccttgaa gaaaagggcc cgaatgttat tatagccgat tttcccttga
     3901 ggtggacggt tcagggtatg ggaaagctgt ttgatgttgg agtaaacgcc attacaccaa
     3961 atcagagaac cataccggca agatatctgg aacctaaggt aaaaaacaga tcaaggcttc
     4021 actacctgat ggcgaatatt gaagtgtctc tctgtagtgg agagaacaac tgggccctct
     4081 tactggatga tgatggtttc gtatgtgaag gaacgggagc gaatttcttc attgttgaaa
     4141 gacagggaag attgattaca ccggaatcac gaaacatctt gaggggcatc agtagggatt
     4201 atgtcaggtc attgaaatct acggtgataa ggaatatcga attgtatgat gtgatgacag
     4261 cgcaagaagc attctttacc ggtacaccat tctgtattat gccatgtact tcaatcaacg
     4321 gtgtaaaaat cggtgatgga gaagttggac cagtaacgaa agaacttcta aaaatatgga
     4381 gtagaaatgt tgaagtggat atcataggac aaataaagaa atgggatgag gacaacctga
     4441 aagacggtgc ttctccttat tcgttcaaga aatgaaaata tttttatata aaaaaggaaa
     4501 tacatatgaa aatatttatg gacggtggcg aaggccatat aataacacat agaatgttac
     4561 cttattggaa aaaaatgggg atatcaattc agcttggaaa tccacaagga tgtgatgttc
     4621 atctatctta tgttcatttg agagcaccat caaaattacc aaaagttctt cgattggatt
     4681 ctgtttatta cgatagtgac actaattaca attcaagaaa tgcgggtata agtgaagccc
     4741 acagaagggc agatgcagtg atatatcaaa gccacttttc aagaagtatg tgtgaaaagt
     4801 atctttctcc aaggaagaag gaagccaaac catttgtcat tcacaatggc atcgacaatg
     4861 gttggtgtgg gaaatttatt gaacacgaag gaaccaatat tattgtcacg gcaaaatgga
     4921 gaaggcacaa aagattacaa gaaataattc agttattctt gaaacactac cagaaatatc
     4981 cttataccca tcttcacatc tttggtctgc tatatcagaa tgttccggta aaacatccta
     5041 atattcacta ctatggtcat gttgacagga aagatttcat gattcatcat tcgatggcgg
     5101 attttactat tcatctttcg aaaaaagatg cctgcccaaa ttccgttgtc gaggctattg
     5161 gggctggaat acctgttata acaaccaatg cctgtggtgg ctccacggag atgtgtgaga
     5221 tgaccgaggg atgtatcata tgtgacggtg atgaagattc tatagaaccc tgtaagccat
     5281 atacgaacgc ctataatatt ttaccaaaaa tattggaaaa taaccttctc gatgcaatgc
     5341 aaaaagttga gaataataaa tatagagtga aattgccaaa ggaattatca gcagaatatg
     5401 tcgcagaaaa atattttgaa gtctttaaga gtatcgtatg aacaatatat tgaaaacatc
     5461 tgacggaaaa gaaccactgg tacatatatc caaattacct gtagtggaca tcgcagttac
     5521 aacatttaac acagaaagat ttgtcaagga ttgtgtcgaa agtattatta aacagaccta
     5581 tccattatgg catatctact ttgtggatga ttgctcatca gacaaaacac ttgagacaat
     5641 aaagagtatt gtgaaaagca ataacataaa agaccaatgt tcctttttcc agacagaaaa
     5701 aaatagtggg tatggtacca cattgaaatg ggcaatagaa atgggggaaa atgaattagt
     5761 ggccatcgtt gattcagatg attttctcga caatgatgac gccttgaaat tggttgtgga
     5821 agaacataaa aaataccctg aagtttcact gatatatacg gactacaatc agattgtttc
     5881 tacccattac acaagaaaag aattaataag agctatgcca cccaccaatg gaaaattctt
     5941 gggaaaattc aaagacggtg tttatatggg aactgatgcc gatatcagcc acttgaaagt
     6001 attcaagaga ggattttaca atatgactat cgggttggac catcgactga taaaggctgt
     6061 agataaggat ttgatactga aactggaaga agtaggcagg atgcactata taagaaaagc
     6121 tttgtattgt tacaggaagc acagtacaag tattacatcc gtttacaggg atagatctat
     6181 cgaatatcac aaaagggtaa tggatttaaa aaacgcaatg tacctgaatg caaaacacag
     6241 aagagaaaga ggaatattat tgagggtcgg tgacacttac acttattctg aaaatggtaa
     6301 aaagatggga ttaaattgta gaggaggatc tgatgtatat tgttagtctt atacctgcaa
     6361 gagagggttc gaagggaata cccaaaaaga atttggcgtt attgaatggt aaacccctta
     6421 tttattatgt aatccagagt agcctgaact caatatgcca cgacaccttt gtttcatctg
     6481 acggcccgga tattttagga tatgcaatgg aacttggaac aaaggttatt gaacagccgc
     6541 caattatttc cggtgataga aattccatcg aagaagtaat aacatatttc acggaacgga
     6601 tcgagtatga tattcttgtt ttacttgctc ccacatctcc acttatagaa tctaaacata
     6661 taaatgatgg agtagaaaaa atccttgttc acggttatga tagtgctatc ggagcaatgg
     6721 gaatggagaa aaatgacatt ctcttttggg atggaattgt aagatatccg gtaaactatg
     6781 aattgaaaaa gagaggtgtg aggcaggaca gagaggagaa ccgatattat attgaaacgg
     6841 gggggttgta tgttacaacc aaaaaacaat ttctacaatc aaaatgccgt gtaggtggta
     6901 atgtcggatt tgtggaaatt cctttctgga accactttga gattgataca ccagaagatt
     6961 tgaagatgat agagtgtatt atgaggggaa aggacagtgc gtgagagtaa aataggtttt
     7021 ttgagaactt atatacctga cagtttctat ggaaaaaata agatgtggct cgattgtgcc
     7081 tcaggtgacg gtgtaattgt cgcaagagat ttattaaaag agaggggaat tattccatca
     7141 aattatattt gtattgatag agatagtgag aaattggaga gtgtgaaaaa gatgggatgt
     7201 aaggcggttt gtattgatct tgaaacctcc aatataaagg aacatgttcc aatggtggat
     7261 ataatactgt gccttgagac attggaacat cttgaagctc tcacggcgga aagattggta
     7321 aaggatttta tttctatatt ggtaaaaggt ggaatgatga taataacatt tcctatcaat
     7381 attgttctgg aagaaaaaga gggaaaatgt tttaataaac atcaacctaa cattaaaaat
     7441 tttttgaaac tcaaggaatt cttttcaggc tttataaaga cgagctggaa caaatcaaga
     7501 gttcttgttt ttatggataa gaaatgaata gtctaacaca agtgaacatt gaattgacat
     7561 ccagatgtga caagaattgc tggatgtgtg gtagaaggga aagagaaagg ctgtatggaa
     7621 aacagggtta tggtgatatg gatttcgata tggttgggtt gatagccgat gaagttccaa
     7681 ccggaattat ggttgccttt cacaacaacg gggaacctct tctctatccc cgtcttggtg
     7741 aggctatatc cctgttcaga gatcaaacaa cctactttgt ttccaatgga ttgacattga
     7801 tgaagagggc aaatgaactc atagataatc ttgatattat ttccgtgtct gtcatagaga
     7861 atgaaagaga agaagtggct gaaagacagt ataacgagat aatagatttc ctggcattga
     7921 agggagacag aaatccacat ttcattttga gatttgtggg gaaggccaat gaagaaagat
     7981 acaaggattt ggatgttctc aaggtcagga gggttcttca tcttccaaag ggaagtatgg
     8041 ggtacagaaa atcacccaca attccagagc acggagtatg tctggattta ttgaacaggc
     8101 tagcaataga cagatttgga aatgtttcat tgtgtgtcag gtttgaccct gaaggtgaat
     8161 tgagattggg aaacatcgca gaaacatcac ttgaagattt gtggaagtgt gaaaagagaa
     8221 agagaatagt agaaatgcac atatccggtc agagacacaa agttccctat tgtgggaata
     8281 aatgtgaatt ttacggcgtg ccaacggggt attgaaatga aagaaggaca tttggatata
     8341 aatcaatttc tatttccaag agaaaaggct tttacagatc tccatcttct tttttgtgaa
     8401 agaaatctca gaacaattct tgatgtgggt gggccctctc gggcatttaa tatatttaat
     8461 atccaaaact ggatttatcg ttattctaat attaccggaa gtcaaaaaag tcattttatt
     8521 caggacgata tttgtaattc taaaatagaa tcggattcat ttgatcttgt atgttcaaat
     8581 gatatgtttg aacatatttt agagccatgg ttggcggcag aacattgtgt aaggatatgt
     8641 aagtcaggtg gtttattatt tcatattaca cttttttatt gggatttaca taattcgcca
     8701 atagattgtt atagatattc acatataggt ttaacacatc tttttgagag aactggaaaa
     8761 gtaaaaacca ttaattgtgg atattatcaa cataaaaaaa gtaaacagag agttatttat
     8821 tttggagaaa aaatatgagc caatttgttg caggtgaata tacttatggt aaaattggaa
     8881 tatttggtga aagtgaacac aatatctata ctggaaaatt ttgctcgata gccaccaatg
     8941 ttgtggcgtt tatgggggtg gatcatagaa cagatgttat ttctacttat ccacttaata
     9001 ttaaatgggg gagaaaagat ataaaatcac atccaataag taaaggggat attcgtatag
     9061 gaaatgatgt ctggatagga aaaaattcca ttttatttca cggtgtctca atcggtgacg
     9121 gtgcagtaat aggtgcctgt agcgtggtga gaagagatgt tcctccttat tgtattttta
     9181 ttggtaatcc tgcacaattt attaagaaaa gatttaatga tgaaaaaata gaaattttat
     9241 tagaattaaa atggtgggat tggaaacaaa atataattca agaggcaata gatatactca
     9301 tgtcagataa catagataaa ttggtaaaat ttgcgagaga gaaaaaatta tatgtctgac
     9361 aaaggagaag aaaatatgaa ctttaaacag acaaggaaaa aatgggcggg aatggagtat
     9421 gtttctttat ttaaagaact cattaggtta cttgaacctc atacttatat ggaattgggt
     9481 gttagtaaag gatttacttt taatcaaata tcacctttgg taaaaagagc aattgccgta
     9541 gatattaatg attgtagacc tcatattata ttgagagaaa atgttgaaat atttaaaatg
     9601 aaaactgata atatggcatt gatgtggaga gatacgattg atttcctatt tattgatgca
     9661 aatcacaaaa aagagcaagt tttaaaagat tttaataatt ttactccgtt tgtaagagag
     9721 ggaacaggaa taatagcttt acatgataca catccaactt ttccagagct gatagatgaa
     9781 aaatattgtt ctaatgcata tgaagcggca tgggaaatta gaacaaatac gatttatgct
     9841 gactttgagg ttctaacaat acccggccca tatgctggat taacacttat aagaaaatct
     9901 aagagacaac tatcgtggaa ataaaacatt acattatatc aagaataaat attggtctat
     9961 attcgacaaa ccctaaaaga tatttagtca ctatggatga aaaaaatact cggatggatc
    10021 ttctttttag ctatctggaa aaaaattcta ttccatcaat agcatctcaa acaaataaaa
    10081 attttgagtg ggtctggctt atagatgacc attcacccga tggagtaaag gaaagagtaa
    10141 aaaagttatc aaacatttta aaatccacga ccatttttga taaatgggaa tcaaaagtgc
    10201 cgtttggaga gggaaattcc tgtggaggca actggcttag acaatttcgc gaaaatttcc
    10261 ggccgggtgg aatagtctca attacacgat atgatattga tgattatata tcttccaatt
    10321 ttattgatgt tattcaaaaa cattttgaat atagagaatt ttgtattgat tttgaaaata
    10381 gaattagtgt cgattggaaa ggagaaaaat atcttaccta taataaacca ccaagtccta
    10441 ttataacatt attagaaaaa actgataaaa ttaaaacacc ttattattat ccacattata
    10501 aactcgattc atactatcaa atgaaaattc tccctgatat agtctatgca aaaacagaaa
    10561 aggagccaca tccttctata atccaatcaa agttgaaaag aaccaatatc aaaaacttcg
    10621 aggctttagg agaatagata tatgttaaat gtagatttta gaatgtggaa agatttcaaa
    10681 aaattatcaa ataatgataa ctggaaccac atgagctttg agagaaattc agagataaca
    10741 tatccttggt tggagaatat taaaattccg ctggatgata atatagttga taatttatat
    10801 gcctcatgtg tatttagttt catgtatcct atacattttc cggctctcat atcagaaatt
    10861 tatagaataa tgaaacctgg cggactaatc agaacttgtg ttccagatta tgaaattggt
    10921 atgaaatgtt attttgataa tcctaatatt ctcttcaaaa aaaggccgcc ggttcccgat
    10981 ttttacccac aaacaaggct tggaagatta atggcttggg gatcagcaca ctcgtcgcca
    11041 gaggtctata atgaatatgt tcaaagacga atttcctttg attttgaaac aatggaatgg
    11101 tatcttaaaa atgccgggtt tatcaatatt caaaaaagaa accataatga ttgctcccca
    11161 atatttgagg gtagagattt cgaagtaaac aaacattttg ttttgtatgt agaggctttg
    11221 aaacctgaca accccataga tatttgtata atggctgtta ggcggccgga gcttcttgat
    11281 acacttctta aatcatttaa agaaaaaatg aatttgaata ataatagaat tattattaat
    11341 gttgatcctg taggagaaaa tattccttca gaaagagtta ttgatattgt ctataaatat
    11401 tttaatgagg taacttatag attatcagat gaggcaaatt ttacaaaggc cattaaatgg
    11461 tgttggtcaa atgttaaaac tgaatatttt ctctcattgg aagatgattg ggagcttctt
    11521 acagagatta atcttccgtc aatgatgaaa ataatgaaag ataacaaaaa tctcgcccag
    11581 ctttctctaa gatgggaaaa atggaaaaag agatatgaac ggaaagccga atattttgcg
    11641 caacctatgt ttataggtca tccttcttta atgagaaaat cttatattga atatgttcta
    11701 ccgttgttag ttgataataa aaatgttgaa gtacaactca aagcgataaa tactctgttt
    11761 gaatttggtc aatatacagg atcagaagaa tttgtaggta atctaaggga tcatggtaga
    11821 gagtggagag aaagtcatgg ctttatcaag acgggcccag aaaaatcgga ctttatttta
    11881 tggaaggaaa gggctataga aaaatcaatt gatagtaatt atgataacag taataattcc
    11941 agttctgaat aatataaatt atacagatga tttgtttttg aatataaaag aaaatactat
    12001 ttcaccatcc gaaatagttt tgattgataa tgggagcaaa gataattata aagatcttat
    12061 taaaaaatat aatgatttga atattaaata tgtgagaaac gaaactaata tcggtgtaaa
    12121 tggcgcgtgg aatatgggaa ttgaaatgtc taactatccc ctacttacca tcttaaataa
    12181 tgatattata attaatagat ttttctttca aaaaattatc aaaacaatgg aagatgtttc
    12241 tataggcttg tgtgttccaa atactattaa aaataaaaat tttgtggatg aaaatgtttt
    12301 acctaaaata gcaaatttag aaagaagaga aggatgggct tttaccataa gaaaagaaat
    12361 agtaactaag attgggtata ttccagatag tctcaagact ttttgtggtg atgattactt
    12421 atttatggcc tcaaagaagg caggctttaa aaatgttaaa atattaaata gtaatatatt
    12481 tcatgttatt agtgctacac ttaaatacga tcaggcgtgg aaagacaggc taaaacaaga
    12541 aaagtctata tggaatagaa tatggaagga gatgaaagat gattgatatt ttttgtactg
    12601 cgatcagacg gcccattatt gttgaagaaa cttttaaaag ttttaaagag aatcttttca
    12661 agaatcataa atgtaggctc attattaatg ttgatcctgt aggagaaaac attagttcct
    12721 atgaaatcgt agaaattgga aaaaagtatt ttgatgagat atatccaaat acccccacag
    12781 aggccagttt ttctcgggct tttaaatggg taatgtcaca aggcaaaaat gaatatgttt
    12841 ttaatttaga ggacgattgg ttacttttga gagagtgtga tcttagtgat atgatcaata
    12901 tcttagaaag aaataaaaat ttggcttgta tacgtttacc aaatgccgat tttagttgta
    12961 aatggccagc gaatataccc aagatttttt tggaggacgg taaaatctct cttgatcata
    13021 gattttgtgg acatcccgca ttatggaaaa tggatttttt tagagatgcg cttaaaattt
    13081 acgatgaaaa tataaatcct gaaagacaat ttaggtttga ttatgaagaa aataaattgg
    13141 aaatgaagaa cctttttaaa aaatggcagc tcggagtatt ttatgataaa ctctatatca
    13201 aggatattgg aagaagatat ggtgggagaa aaaatcgggc acctaagata tatgagtata
    13261 cagaaaaata caaatattta gtaaaatggg agatatagtt tttctaaaat gttttataaa
    13321 atctctgaat tgaaaagaaa tacgaatatc ggcatcatcc ttggaacggc gaaatccgtg
    13381 aacaacattt cacaaacaga atgggatgaa ataaacaagt gcgacaacct tggtattaac
    13441 aattggtttt atcatcccac attcattcca aaaatgtgtt cattggagtt gaagagctac
    13501 gattggcaga tatcaaaaga caggatagca gaaaaatggg ataagggttg gaaaaatgtg
    13561 aagtggataa tctccgaaaa gaaacacgaa tatatttctg gtgcaatcgg acatgaaaat
    13621 gaggctcaga tttatcttta cgattttgag ataagggatc agaaattaca tccaaagttt
    13681 gtgaaaaatc acaaatacaa tgccgacttt aatcctgatg tgggcccctt agtgaaaagt
    13741 tatgatgctt cacttacaac acttgtccac ctgatgtata ggctgggtta taaatatatt
    13801 atattatacg gtgtggattg gacacatagc tactacttct ggtcatcggg agatgagata
    13861 tacggtaaga cacattgtaa aacaaataaa gaccacgaag gtaaagatcc aaaactgcca
    13921 cacaacacag agcacgttat agattacatc atagatttca atgaaaggca tatgaaacca
    13981 cagggaaggg aaatccttgt aggatacaag gaaacaaggt tataccctca cctgaattat
    14041 atagatgtgg tgaataagga aattcacagt agaaaatgaa aaaaaaaaca atctggaaat
    14101 acaaaaatat atagtaatgt tttgttcgcg gtcttgtgca tcaatccata gtaataaata
    14161 gagagggtga tatagaaatc tgacctttca ttgtggtagt taattatttt gttgaaatgt
    14221 gaatatgaaa aattattgaa aaaaagtatg agggaagata atgggtaaga ttattcttgt
    14281 agcagaatgc ggcataaatc ataatggcga tcttgagctt gccaaaaggc ttataaagaa
    14341 atcaaaagat gttggaatgg attatgtgaa atttcagaaa agaaccattg accttgttta
    14401 ctcaaaggaa gaactggaaa gttacaggga aagtccgtgg ggcacgacat tccgccagca
    14461 gaaagaggga ttggagttta acaaggaaca atacgatgag attaacagat attgtaagga
    14521 aattgggata ccgtggtttg catccccgtg ggatttggaa tctataaatt ttttgacaca
    14581 gtataatcca ccatatatta aagttccatc tgctcttgtg acttatagga aatatcttga
    14641 agcaatagca gaaacaaaaa ttcctgttat aatgagtaca gggatgagta ataaaaatca
    14701 aatagacact gcaatggata tccttggaga gaatcttgaa attcttttac atacaacagc
    14761 aacatatccc tgtaaatttt ccgatttgaa tttgttaaag ataagaacat tgataaaaga
    14821 atatggtcat tattatacca ttggatattc caatcattct ccaagtgcaa tcccgatttt
    14881 gttcgcaatg gcattgggtg cagaagtgat cgagacacac attactcacg atagatcagc
    14941 atatggttct gatcaatcct ctgcactcga aaagagacaa ctggaaacaa tttgtgagtg
    15001 gaggaatgag ttgaaagaga cactcggaaa tgggaaatgg aaaatctttg attctgaaat
    15061 tcccgttatg aaaaagttaa gaaagcatta tgacttttag gaaacaaatt ggagataaag
    15121 ttgtaatatc aaatattttt catgaacatt atcgagaggt tggattaatt gttgatattc
    15181 gtggtaaatg ggcgatggtt ttactggata atcagggcaa aatcatcgtc attttacttt
    15241 ccgatatatc aaaaagattt acataacgga aataatgtga tataataaat acatgagaga
    15301 aaacataaag aattttacaa agtatttgag tgatttggaa aagatattaa gtaaagggga
    15361 ttatgaaagc attcttcatg tcttttccct gatcgttcaa gctcggctcg caaaaattag
    15421 aaaccagatt gaagatgctg aaatcatacc ggagcagatc gaaaagccga agaaaaagaa
    15481 aggagtagat atatatgttt gatgatttat ttgatgattt aatccagaag aagaaagcac
    15541 cggaagtcaa acaaccagag ccggaacagg ataatccatt tggtgagtat tttgacaccg
    15601 gaatgggtga tacccatgat gatgtgtggt caagcggtca attgcctgat tactggaaaa
    15661 caaaataaaa ggtatgattg atcttatagc aacaaaaaga cccgatgttg tttctgtcag
    15721 gataagcatc aatgataatt atgtcactaa tggctttaca aattcctcaa tgacgagttt
    15781 ttttgtggtg agatattctt cctgtccgcc ggcagaaatg tcggacgggg atgaaatggc
    15841 cgtgaaaaag gcattagaac agagttgacc gatatagcca aacttataca tcttcaacca
    15901 cattgcccaa tctgacaggc ggtgtattct tttatcagtt acaaatcccc caaccttttc
    15961 tattacatcc gatttgtata aactatttga tgaaatataa ttctgattca ttaggaagtt
    16021 tatatcatag acctttgggg ggaaaatgac attgatatat cctttatatt cgaatggaga
    16081 ataaacgaaa ccaatgttac catctgtttt ttctattgtt gataacattt tgtcaaggaa
    16141 attcctacca aggactatat cacggtcgag tatttggatg taaggtggga atgatttgaa
    16201 ttgtttcttg tattcgtcaa gacctgcttg acaattctct gcgtgtttgc cggggccttc
    16261 ataacttgcc cagataatag gaacattact tctttcaagg gatacttttg tctgtttgaa
    16321 tatcttatgt cctgtcaata agggggtagc aaccaatatt ttagcgttct tatcttgtcg
    16381 aagtattttt aacatatttc tttattcaga aaccttttct ggaaacattg tggcctcgac
    16441 cgtatcatcc gttatatgcg gcccggcagc cgtcaaatca tcccctacat caggaagatc
    16501 ggtaagttgt ttatcacctt ctcctacaac aggattctct acgaagccac aaaattgggc
    16561 tgaaaaccta tcgtccttta taatgtgatt gacaatatat tttctgccca tccattcaat
    16621 aatctcacca aggtctggta tcttggaggc cttcgctgta aatctcaact gtgcgtaatt
    16681 cacataatca atcttgtaag aaccgatttg tgttcccgtc acaggcatta cagccttcct
    16741 gaattgtttc cttgaaatcg gttgttttgg actattcatc ttcttctcca ctactataat
    16801 tcctgatgat tgcctccatt tgtggtgcac tgtcttttag agcctcaaga tagtgtctca
    16861 tacccacttg attataagta tttgtctcat ccagtaggct tattgtgttg agtgcgaata
    16921 gagcatcggc taaaccacta agtttctgat gttcaatcat caggtcatca atattaacat
    16981 ctttgagagt caaacttact tccggcttca cactactgaa gttacccgta ttgagtgtgg
    17041 ccgccttcgt tattgttata tccacctttt ttcttgacat tttactctac ctctatttct
    17101 tttcatcatc cgtttttttg tatggtattg caaacctctc ttctctaaac tccattactc
    17161 aatctccttt tttataaaat ccacagtatg ttccagtttg aactctggat ataatttatc
    17221 tatacggtga taaacatttg cgatatagcc aagatacaca aaatcatagg tctgtgcaac
    17281 aaagaccata tcgttcaatt cctttataac cgtatcagag aacagggcgt gataataacg
    17341 atgttcttct ggtaataata ttataacatt gttctcaagg attgtaaata ggtcgggatt
    17401 tagatatccg atggtataat cacgaacaga accaagtaaa accgtgcatt ttatatcact
    17461 atacttcaaa ttcttgtcca atatgacatt cagattggta tattcctctg tcttttcttt
    17521 cgaaatattc gaagaatccg agtattttgt cgaaaaagag gggtaattct taccaaattc
    17581 taccagatat ttctcaaaag agttcaattt atcgaaaata atacccttaa aaccgattgt
    17641 aacggcccgt ggctcgactt tatttagctt tagggtatca attgagtgga tttttgggca
    17701 aaattgggga aggtaacgga aatcgggcct gtagttgatt gctggctcca gcaacttgac
    17761 attctgtttc ttgagccaac gagcttcatc aggttttatc gcaagatatt tgtcgaatac
    17821 aacaacgggg attttcttct tgagataaaa cttgaatgtt tcggtttgaa ggacaaaggg
    17881 ttgggggttg tttgaattat agggaatata ctcgatataa acagcatcta tattgtctgg
    17941 tagatttttt tcactttctg gaattataac attgaatgat tttgatagtt cctttgataa
    18001 agatttatca acattcaata aaaatagatt tctcacactt tcttctccaa aattcttttt
    18061 tccgctcact catcagtttc ttgtttctgc agaatgtgtt ttactaatgt gagccttttg
    18121 cattttctct ttagaaaccg atctttcttt ttccatttac atttatataa aatcataatt
    18181 caagatatac aatgtcatta ggaatgtttt tcacaaactc cttctcatat tcaatgatgt
    18241 aggaaataac agcttcagaa aaaccgtcaa tatgaaccca atcaccatag cccacaccat
    18301 tacgatatga ggccacattg atcaagtagt aaagagcctc cggtaaattt acaggaatat
    18361 tgtcgtgaga ctgctcatct gtgataacaa tcagacggtc aaacttttct gaagaaataa
    18421 tatgcttgat tgcactacca aggtatgttc cgtcatgtgg ctgtgaacga tgaattgatt
    18481 cagcaagaga gaaaccacga accgaattag acaccatcac cgtcttgttg gagaatgtat
    18541 aaatagaaac attttcacag atttctcttg caataagagc aataccattg gcggcatcaa
    18601 tccttgtcat atctgactta ttggaaagag ccatatccat tgaaccggac acatctacaa
    18661 gaatgatggt cttaccatcc agcatttcaa ggttctcgat agacttgaac atcaactctt
    18721 caagcttttt cttgaagaaa ggagcatact ttgcagccgc gataaatctg aaaggaagaa
    18781 ccttttttgt gttcattctg tcaaggccac gaaggatgat gtttctatct actttggcac
    18841 tttccatatt acgaagattt ctcaagagtg caagggaacc gaggcggttc tccataataa
    18901 gagtttccca aactgttttt ttatccttcc cagcagaaag ctcagtttcc caagttttag
    18961 gaactgctaa ttcattattg atgagtttct tccagagaag agcctgttcc tcgttgagag
    19021 gtttggcgtg agaaaggaag agaacatccc tcaacttgat ttggttgtta cggttgtatt
    19081 ttgcgagctg atactcgtca aatttcgtga aggccctggc aagacccttc tttacctgtg
    19141 aagcaagagg aactttcccg tctttccagt aaagagaaag aaattccgac aattcatcag
    19201 cacgctgaat aattctttca agagtttttg ctacatattt tctgtggtca atatgacgcg
    19261 ccattcctgt agcaaggaga agaggaacat gacgcaaatt cattttctct cttgcctcga
    19321 cagcaaggtt agccacaact ttaccattta cttgtgggat tgtagcaagg atcctgtccg
    19381 cgatgttttc cccgttctca tagaactggg attcccaaag caaacaagac atagttgaac
    19441 gcctgaggat ttgctcgggt gtaagaacct ttgcagaagc accctcatag gtcttaggaa
    19501 cacaattggt tttcaaagga atattgtatt tcgccatttt actaacctcc tacaaattta
    19561 tgttggtaac ggggaacatt cgaaatcaga aatggcctca catggggccg gccgttgttg
    19621 cgaaacgaag taactgacct ctatcactac cattatattt tttgacactc cccgtgacta
    19681 aagttagggg attctttggt agtagtcgga agtccatttc tggtatccgt agttccccaa
    19741 agtttagggt gtgccatcac ccctcctaag acagtgcata tagcatcttg ggctggcaga
    19801 ctatcgctgt ttgctacacc atctgccaca ggtgctaaca ttatatttat tgcacctagt
    19861 aaagtgggga acatacgaaa agagactttg tttccatttg ctctaccatt gagctatctc
    19921 cccataatgg ggagagttag aatcgaacta acaacctaat ggttccaccg aagtaactct
    19981 attctacacc accacttcaa atattgaaat gttagggaac aattgtaaac ggtctacggt
    20041 cttttgtcat catcaggcga agtatccgat tactacacca ctaacattat aatctgttca
    20101 tcggggatta agtggaaatg gccgagaaaa ttacatctcc cgaaaaaaca tttccatata
    20161 aggctactac ctaaagttta tttgtctttc cggttcaagg agggctccta cgaagtatcc
    20221 atttcctgcg ccaccgatga atgttaaatt gtcaaagaat agtattattc taacatatta
    20281 tttgggttat gtaaacaaat tattttcttt tgagtgaaga tttcttcact ttttcttcaa
    20341 tttcttgaga ttcctcggcc ttttgtaaca tggcctcttc tttctcttca gattttttct
    20401 taccgatggt cttttcaact acccaatatc ttacttcatc tgatatagca tcaactcttg
    20461 atgcaaaacc cgaaacaaga acacggtgat ttggtccaac ttttttcata agagtcgcaa
    20521 gggcttcctc gtgcgaccaa gcaacaataa atgcagtcca atttgacata ttgtggagta
    20581 attcgtattc aatacgaaat acgcccattt cttttttttc tgacatattg cttattcctc
    20641 caatctctct attacaggta catactctat tcgtttatct cctttaattt caattatctt
    20701 gaaataggta atcaggtctc caactttttt tgtatctctc tgtactacaa ttgatttacc
    20761 ttcgaggaaa cattctccca ctacataatc cttggggcct ataggaagtt ccataaattc
    20821 cttttccttc ataaaacctc acttattttt tcttcttctt atcctttttg gttttcttct
    20881 ctttctttgc cggtttcttt ttatctgcca taggtcatca cctccatttt tatttatgaa
    20941 gatatttata taaaaaatta aaaccactaa acttgtttag tggttcactc ttaaccttgc
    21001 tcattaatat atttctgtaa tgtctgctct gaaacattgc cgatagtaga tacaaaataa
    21061 ccatcagacc aaaaagtatg ttctttccaa aaatatcttg tcaatatttg agaataattt
    21121 ttccataata ttatcgtaga ttttccttta agttttttaa caatatataa tggacttatt
    21181 tttggttcag cttgaactaa taaatgtata tgatccgtat caacttccat tactttaatg
    21241 atataattat cattaataga taattgacta catatatctt taattggaaa ttgatcaata
    21301 atttttaatc tatactttac cacaaatata atatgatatt gtaataaata ttttgaatga
    21361 tttttagtta gatattccat acaaaacttc caaaattgta aatcttatct attaatgtaa
    21421 acttaatata ttctttcttt ttagagtcac caaataaaac atatttacct ttattaaaca
    21481 tccctttgca agtatattta atattatttg accaaaacaa atctttcggc aatattttac
    21541 ttctttctct tttaattgat ggtttaaaac ctttcctatt taactgcaaa accctattat
    21601 ttctatgtct ttgctctata attataggtt tacatcttac ttgttgagtt ccacctgcaa
    21661 taataaaagc atcattgaaa tgagtttttt ctatattata ttcgttatgc ttcatttgtg
    21721 taatataacc ataagttacc tgcaaacctg taatatcttc ataaaatctc ttatgtatta
    21781 tagacataaa agttgaaggc tcataccttt taacaggttt ttgaaactct ttttctctat
    21841 gtttttcgtg taaatctaca tggcaacttt tatgtaaaag caataaacca tcaggtctat
    21901 tagatcttct tgaatctcca tgtctataaa tatgatgtgt agcttttggc tcacctttaa
    21961 attctttttt acaataagga cagatattat tttgccttac ctgtaaataa ctaaccatat
    22021 tctgataatt aaaaagattg ccttgctgat attctttacc attaattttg ggattcatta
    22081 ttttttgtat atcaaatttt gctacctcaa ctataacatt tttaataggt aatagatttt
    22141 taattttttc tatcaaagtc aaatgagttt gatattttct ctcaattgat ggtggtagcc
    22201 aattatcttt tcgtttccta ttattaaatc ttgactttct atatcttaac ttatttctgc
    22261 gattcctgcg atacatcttt ttctcattaa gtctatcttt agtctttaga tcaagattta
    22321 atgttcctga aaacaactct tctttttctg ttatacttga aaatccaaca tgcttataac
    22381 cactatcaat gcctaaatta atatcctgta ctgtctcacc agtacccata gttaatcgaa
    22441 tcgtaaatgg aaatcttttt acaacattag ctttacctcc ttttaacaat ttcttagctt
    22501 tttgtggttt acatggcatt aacgtcttgc cactcataga aagtacaaag acaatattcg
    22561 atactctcaa gttcgaatcc cctccttgct ggggttgatt cacttcgggg ttgttacaaa
    22621 tcagtacttt gccatgagca ctgagagttt cctctctgtt taacgcacga cttgtagagc
    22681 tacagactag tgaagcatcc gtaggtacgt tcttgaactc tctttgtaac gtctgcataa
    22741 ttccttccaa attaagcccc ctaatcaacc aattaccctc aagtttcaca actcaagccc
    22801 ctgaatttat tcaggggtga ttgacttatg tttcttcttt ttattattat ttattttact
    22861 tatttcctct tcagcaatat cttcaagttc acctatggca gcttctgttt ctttattgat
    22921 ggtagaatag cccgtttgtt tcaaccactc attaaacttg agaagaacct cgggatattt
    22981 agaaagatta gcctcggatt tatcccaacc aattcccaat ttttcttctt tctttccatt
    23041 tataactgta tatgttttac cgtcctcttt tacaagtcca gcagtaataa gatgtgtcaa
    23101 taatccagca tagggattga taccgttgac ataatctata ctgactgttg cttgctgaaa
    23161 cggtggatac attctatttt taattgtaac ggctgtaatc tcgtttcctg taatcttctt
    23221 atcaactttt atgggtgtct ttttgagatt gatgaggata gacggaaaca acctcattgc
    23281 ttttccacca ccaacctgat ctggcattgg aatgacacct ggcaaaccat aaaggtgacc
    23341 agttacaatg ccgatgcttt cctgttcaat acagatgttg agaagtagtt ttaataatgc
    23401 cctgatattt ttctgtaaca atccttggtc agcttttgga tcaccatcaa gggcatcctc
    23461 atacatcttg tatctatcaa ctccacccat cgagtcaatg ccgataatca ttctcttctg
    23521 accagttcct tttatctgag caagaatact tctgatttca ttttcccaag gcgtgtatat
    23581 atattgggta ttctccacat caagccccca ccttatacaa aaatctttat cagttcctct
    23641 ttcggtatca ataataattg ggctatatcc tttcttttgc gcctcggcca tgcaaagaac
    23701 cataaaactt gacttcattg tatgctctgg tcccacaata ccaacaaggc ttctattcgg
    23761 gataccctta ttaatatccc ctgataaaat tcggtttaag tccagacttg gggtttttac
    23821 ccaatcagat acagaagcaa tttccgattg tgacattatt gatgcgtgaa cacccttcac
    23881 attcttagct attctctttt gtaaatcttc aaaaaaactt ttattcgaca taaaaatccc
    23941 tcattgttct tatattatat catgttttat tattattttg acactccccg tgactaaagt
    24001 taggggattc tttggtagta gtcggaagtc c
//
LOCUS       DAOTQL010000009        23528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2212_length_23528_cov_5.641929, whole genome shotgun sequence.
ACCESSION   DAOTQL010000009 DAOTQL010000000
VERSION     DAOTQL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_23528_cov_5.641929"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..939)
                     /locus_tag="P5293_01120"
     CDS             complement(49..939)
                     /locus_tag="P5293_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRR48568.1"
                     /translation="MVAEFIEYLKTQKRYSYRTQAIYGEYIREFFEFASDGEEESVDV
                     LKPNLIRGFIAAGLESGISPRTMNLKLSALSSFSNYLVKCGILDSNPVKRVHRPKQSK
                     KLPQFYTEHAMENYFSKEVCKDYKSLRNRAIVSTLYNTGMRRAELANLKVSDWDMNRS
                     LFRVTGKGDKQREIPITNSLREELERYLACYSKDFPVTSISPLFVTDSGKPLYLAFVN
                     NVVRRELLGVEGFSGKKSPHVLRHSIATHLLNNGADLNSIKEVLGHSSLAATQVYTHN
                     SFEQLKKVFKTAHPRAKKGG"
     gene            complement(1028..1219)
                     /gene="rpsU"
                     /locus_tag="P5293_01125"
     CDS             complement(1028..1219)
                     /gene="rpsU"
                     /locus_tag="P5293_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRR48569.1"
                     /translation="MIIVPIKEGENIERALKKFKRKFEKTGVIRELRNRKAYVKPSVK
                     RRDEIKKAIYIQSLRRNEE"
     gene            1325..3364
                     /locus_tag="P5293_01130"
     CDS             1325..3364
                     /locus_tag="P5293_01130"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRR48570.1"
                     /translation="MELEEFRKAIEKGIPDTLPEPKPYDNNINHAPKRKDILTNEEKE
                     LALRNALRYFPKKHHKILAPEFARELQEYGRIYMYRFRPDYKIFARNIDDYPHKSRQA
                     AAIMMMISNNLDCAVAQHPHELIVYGGNGAAFQNWAQYLLTMQYLSQMTDEQTLVLNS
                     GHPLGLFPSHKDAPRLVITNGMVIPNYSSKDHWEKFNALGVSQYGQMTAGSFMYIGPQ
                     GIVHGTTITVINAARMHSKKGDEGFKGKLFVSSGLGGMSGAQPKAAVIAGVIGVIAEI
                     NPKAVQTRYSQGWVDEVFTDLDLLVERVIKAKNNKEAVSIAYQGNIVDLWEKFIEAGV
                     DVDLGSDQTSLHNPWSGGYYPVGVSFEESNRLMAEEPDKFKALVQESLRRQVSAINKL
                     SKKGMYFFDYGNAFLLEASRAGADVLYHDGKFKYPSYVQDIMGPLYFDYGFGPFRWIC
                     TSCKPGDLRVTDEIAAKVIEEIINHAPEEIKIQLSDNLHWIKEAESNKLVVGSQARIL
                     YADCEGRIKIALAMNKAIREGRISAPVVLGRDHHDVSGTDSPFRETSNIYDGSNFTAD
                     MAVQNVIGDSFRGATWISIHNGGGVGWGEVINGGFGLVIDGSDEAEKKIKEMILWDVN
                     NGITRRAWARNSGAIFAIKREMDKTPLLQVTLPNMADDDLISETAKHHFYGKSQI"
     gene            complement(3314..3829)
                     /locus_tag="P5293_01135"
     CDS             complement(3314..3829)
                     /locus_tag="P5293_01135"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRR48571.1"
                     /translation="MKYLISAIFLFAAASCSPTYVVTETAKPFEIERYMGRWYEIERL
                     PNKIQEGLEECTSLLTLMEDGSFTIVNEGRLISDKTRIKQWKGKGWIPDRRNPSKMKV
                     SYNWPFTEDYWVLLIDNDYSIALVGSPDGDLLWILSRDKDVEQNTVLKLKDYASDLGF
                     PVKMMFRSLAD"
     gene            3939..4787
                     /locus_tag="P5293_01140"
     CDS             3939..4787
                     /locus_tag="P5293_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HRR48572.1"
                     /translation="MKRNIIKIDEKLCNGCGSCVQGCNEGAIQLINGKAVMISELYCD
                     GLGACIGECPEGAIELVEKEAEPYDEIAVMKRIAINGEEAVIAHLKHLQYHDETEWLN
                     QGINWCKENNYLIDLSKLDNYKPRMACGCSGSLEKDFKQPESSNNRDNMSSNNMTELK
                     QWPVQLHLLNPQASFFHEADVLLASDCSAYTFSNFHDKFLKNKSLAIACPKLDNNIQS
                     YIDKISEMISVSKINTLTVLIMEVPCCSGLLKIAVSAREKSGKNIPIKAVTLSVRGDI
                     IDNKWV"
     gene            complement(4796..5167)
                     /locus_tag="P5293_01145"
     CDS             complement(4796..5167)
                     /locus_tag="P5293_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRR48573.1"
                     /translation="MERLCAIRDLCRALCSYEESFHNMYGICLNEGMLLCSLKDGQLS
                     SGEIADKLSLSCSNTSKLIRSVEEKGLITRAMGERDKRQMYFDLTDEGRQTLNLILKD
                     ELELPEPLSLFINKYKSSQLL"
     gene            complement(5213..5734)
                     /gene="trxA"
                     /locus_tag="P5293_01150"
     CDS             complement(5213..5734)
                     /gene="trxA"
                     /locus_tag="P5293_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRR48574.1"
                     /translation="MCKVTKYLIFLMFPILIISCTSNNGNSASAKNGISQNNRIQNNS
                     SINNINEKRMKTIHLTKEEFLNKVVDFENNPDEWKYLGDKPALVDFYADWCGPCKSIA
                     PVLEELAAEYEGQIYIYKVDTDAEQELAAAFGIRSIPTLLFIPMKGNPQVAQGALPKA
                     NLKKAIDEVLLNK"
     gene            5853..7547
                     /locus_tag="P5293_01155"
     CDS             5853..7547
                     /locus_tag="P5293_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR48575.1"
                     /translation="MKYIIIGGVAGGATAAAKIRREDEHAEIIMFERGEYISYANCGL
                     PYYIGGKIEKREDLIVQGTTTFSNRYDIDVRTFNEITSIDTSTKKVTVRNVQNGKIYF
                     ESYDKLLLSPGAMPFKPSLPGIDDPRILTVRTIPDTDKIKSLTEKTKKGKAVIVGGGF
                     IGLEMAENLKALGFMVTIVEMIPQVMTQLDPVMASIVHNELRENGVELILGEAVMAFR
                     PTSEKLYVQLKSGREISADLVILSIGVKAETSLAREAGLKIGITGGIEVNDYLQTSNK
                     DIYATGDAVEIINPITGDPSLTPLAGPANKQARIAATNLVHGNICRYKGSIGTGIAKI
                     FRITAAVTGVNEKTLNKKGIKFISTITHSGSHAGYYPGSESLSIKILYSPKNGKLFGG
                     QVVGYGGVDKRIDIIATIIGQNGTVYDLMEIEHAYAPPFSSAKDPVNMAGFVADNVIN
                     GKVKIVTWDIINNFDFGNDYLLDVRSPEECKQGMIKGAVNIPLEELRLRLDEIPSDKR
                     IIVYCAIGMRGYLASRILLQNGYGNTYNLTGGYKTYLFAHKDQEIMTKLGDTKNKS"
     gene            7544..8104
                     /locus_tag="P5293_01160"
     CDS             7544..8104
                     /locus_tag="P5293_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRR48576.1"
                     /translation="MKHLAKFLLKIMGWKALTPPVPEPKCIILGVPHTSVWDFVISYL
                     YYKSFGATAYIMIKKEFFRGFLGPILKSMGGIPVDRKHPSTITRQVIEAFNNTDHLHL
                     AIAPEGTRDKVEKWKTGFHIIAKAANVPVYLGYFDWGKKEISRGIKFELSDNVNEDLR
                     KIRQYYKDLGVKGKHPENFTTGSDLD"
     gene            complement(8110..8943)
                     /locus_tag="P5293_01165"
     CDS             complement(8110..8943)
                     /locus_tag="P5293_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR48577.1"
                     /translation="MRLKVILILLITALFLPYNTIAQEIVILHTNDVHSNVEPLSGGR
                     NKGMGGAQRRANYIQNVREREDNVLLLDAGDYNQGTPYFTLFKGEMEVMLYNAMKYDA
                     VCLGNHEFDNGEAQLAERLSKAKYPTLCANYDFSKSPLKDLVKPYVIIEKGGKKIGIF
                     GLLLDLNGYVAESARSGIKYLGVIRCANDMAKYLKYKQGCDLIIALTHLGYNHDEDSR
                     SPSDIDLAKSSRDIDIIIGGHTHTFLEEPTVVKNRSGRGVMVTQMGTAGVYVGRIDLN
                     F"
     gene            complement(8943..9692)
                     /locus_tag="P5293_01170"
     CDS             complement(8943..9692)
                     /locus_tag="P5293_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRR48578.1"
                     /translation="MFKKAILFSLSIFFALNTFAQETTFQWKATRVLMDSTWNFPEEL
                     VVKGIIDFYKPQVDSQMQQVVGISEQEMRSGRPESLLSNFTVDAMLEIAQRKSENNVD
                     FALTNFGGLRASIPAGEVRRYDIYSTYPFDNYLVIIDLKGSSVKKLFNILAASIEPLS
                     SNVFLSVKSGKLNYALINGVQIDDRKTYRVATLDFLMTGGDNLYPLKEAVKVEQTGML
                     LRDVILEKIESLTANNQKISSKITNRIKIAQ"
     gene            complement(9705..11021)
                     /locus_tag="P5293_01175"
     CDS             complement(9705..11021)
                     /locus_tag="P5293_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR48579.1"
                     /translation="MDKNKLFLIISREFSVRVKKKSFILLTILTPIFMALLIVMPTII
                     MSFSPSEREQKILVVDRSRECEKYFVSNEDYVYRFDPNADIQSIKKDLATSDLYAIVE
                     ISPMDSAYNVSVSTYSNKQINIDTKEEIKKCVERGIRDKKLAAYNIQNLDSIIKDINT
                     KIDVKTYTVSEEGKEKSGMVEINMAISYLASFLIYMFIFMFGSMVMRGVIDEKSNRVV
                     EVIISSVKPFELMLGKIIGVGAVALLQFLIWVALTFAIIFGAQAFMGVDLASTGGSVP
                     GALPEGFSASGGKVGEMLTALAGINFGYIILSFLAYFILGYLLYASMFAAVGSAVDNE
                     TDTQQLTIPITLPLIIGLFLMIHAFRFPDSSLSFWGSIIPFTSPMVMMARVPFGVPLW
                     QMILSIMLLLATFIFMTYLSAKIYRVGILSYGKKATWKDLVKWLKF"
     gene            complement(11032..11940)
                     /locus_tag="P5293_01180"
     CDS             complement(11032..11940)
                     /locus_tag="P5293_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR48580.1"
                     /translation="MNVISCNSVVKKFNQFTALDGVSIEVPQGTIFGLLGPNGAGKTT
                     LIRIITQITIPDSGEVRFCERPITISDVTRIGYLPEERGLYKKMKVGEQSVYFARLKG
                     MSHAEAVKELKEWFIKFGIQSWWNKKVEELSKGMAQKVQFITTVLHKPSLLILDEPFS
                     GFDPVNVQLIRNEILKMKDNGTSVILSTHNMESVEELCDNIALINKAKLVVSGGVDEI
                     RKNYGKNEVEIIYRSSEIREIVSNGFYRVVSNDIYKDGYRVIIEVDREASSSQVLMEL
                     LKQNIDIVEYRELVPRMNDIFIKLVS"
     gene            complement(12004..12756)
                     /locus_tag="P5293_01185"
     CDS             complement(12004..12756)
                     /locus_tag="P5293_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRR48581.1"
                     /translation="MWRIKISKTRASGVMTLIIVVLTIQLILFLFNKQEDNSLNKSLQ
                     PEKVAESDSASYSAKHLKSYGERPKIRIPNTTAQLPAKSAKLADSSAKWKSYRDIALE
                     NMTNVRTDNLKSEKRILKSIELNSADSATLVTLPGIGPYYAKKIIQYRDKLGGFADKR
                     QLMDIFGIDEERYSMFSDKITVDTNKIQKINLNEATYEQLSTNPYIGGYLARSIIRFR
                     DSRSNDIINLASLVVENIIKNELYNILKFYFQ"
     gene            complement(12781..13455)
                     /gene="rsmI"
                     /locus_tag="P5293_01190"
     CDS             complement(12781..13455)
                     /gene="rsmI"
                     /locus_tag="P5293_01190"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRR48582.1"
                     /translation="MAKLFIVPTPIGNLEDITLRALRVLKEVDTVLAEDTRTSSHLLK
                     HFGIATRLESHHMHNEHKNVDFICSRILGGENIALISDAGTPGISDPGFLLVRLCRDK
                     GIEVETLPGAVALIPALVNSGLPCDRFCFEGFLPIKKGRISRLRELGQETRTIVLYES
                     PYRLVKCLSQLVEYFGEDRKACVSRELTKVHEENRLGTLIELRDWYLEHEPKGEIVIT
                     IAGKPD"
     gene            complement(13412..14077)
                     /locus_tag="P5293_01195"
     CDS             complement(13412..14077)
                     /locus_tag="P5293_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48583.1"
                     /translation="MSNNSKIEEIGIILDQISQDIEALKEDDKIGPARWEELILAAES
                     IKCKINSLYLEQIELSLSELKSLKSKSKTDISENDCSLTSFSVNDHKKEKETVAIDDE
                     EEFELFPDSGESILDVARGKKPAWMTDIPGPKITDITDGITLNDKLLYIKELFNGDEQ
                     QYRLSLQKINEMTSLEEVMDYTKNAFPDWDENSSSVYRFYMNIRRKFNGKTIHSAYAY
                     RKS"
     gene            14118..14687
                     /locus_tag="P5293_01200"
     CDS             14118..14687
                     /locus_tag="P5293_01200"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRR48584.1"
                     /translation="MFLENTKKSGWIEIICGSMFSGKTEELIRRLRRAKFANLKVEIF
                     KPKIDTRYSESEVVSHDANAIRSTAVDSPLSILLYASDVDVVGIDEAQFFDSSIVDVC
                     QQLASNGIRVIVAGLDMDYLGNPFGSMPELMAVAEYVTKVHAICVKCGDLAHYSHRLT
                     KNDKLVMIGEKDTYEPVCRHCFNDLRKID"
     gene            complement(14694..15152)
                     /locus_tag="P5293_01205"
     CDS             complement(14694..15152)
                     /locus_tag="P5293_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48585.1"
                     /translation="MKLKTFFAVAASVVALASCGQKAKPVSEQLVGQWTGTDSISITV
                     VDSTGNQVAQEFVAPIDFEYFADSTFTGVIKINDSTSINVGGVATIAEDVVTFTGSMQ
                     GVEPMDLSGSMTYVAEPEGLKITFTAVNAAANTTHSGKANLTRKAAPVQQ"
     gene            15261..16349
                     /gene="alr"
                     /locus_tag="P5293_01210"
     CDS             15261..16349
                     /gene="alr"
                     /locus_tag="P5293_01210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRR48586.1"
                     /translation="MTYSKLAINKENLRHNYDYFRSKLAQSTKLLVLVKANAYGHGDV
                     ECSKLLEEFGTDYLGVATVEEGKRLKNEGIKLPIIVLTPGNDNFEEIIKYSLEPSIVN
                     FDSLQSFVTSLKTLSKTKYPAHIKLDTGMHRVGFVKSEIDLLSNLLKQNPYIEVKSVF
                     SHLAAADEAVHDEFTRSQIELFEEIYANLSDKIESKPIKHILNSAGIERFSYAQFDMA
                     RLGIGIYGISYVNPDCLKPVGFLKAPIIHIREISEGTVGYGRHGDLNGKAKKIATIPL
                     GYADGVNRHLGRGSASFSLNGIRVPTIGNICMDATMLDITGVDAKLGDIVTIFGDNPK
                     AREIADILKTIPYEIFTSISARVKRVVE"
     gene            complement(16355..17395)
                     /gene="holA"
                     /locus_tag="P5293_01215"
     CDS             complement(16355..17395)
                     /gene="holA"
                     /locus_tag="P5293_01215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRR48587.1"
                     /translation="MAGKEKDTEKEFRKILANIKAGEFAPFYLLMGDEPYYADVLIEE
                     IINRAILPEEHDFNLLILYGMDVSSKDVVEASMKYPMMAARQLVIVKEAQFLDKMESF
                     EKYFSNPLSTTLLVLAMNGKTIDGRTILYKKALENGVLLETVPLKENKLVYWIDDYVN
                     SAGKKIDADASMLMADYCGTELRKLAHELNKLITNLDASESLITTRHIEDNIGISREY
                     NLFELTKALSFKNRAKAFKIASHFGDSPKLYPLVVTLAVLFSHFAKVLKYHSLVSSNS
                     SVDNNTICTALGIKTYHLQEYVIASDNYSYAKCMEIISLIRKCDSVSKGNERGEATDK
                     ELLTELVFRITN"
     gene            17479..17883
                     /locus_tag="P5293_01220"
     CDS             17479..17883
                     /locus_tag="P5293_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRR48588.1"
                     /translation="MKEQVFDPVRRKKVALTPEESVRQQLIYYLSNTLGYPISRMVCE
                     YSVEINGLKYRGDLVIMDKELNPFFLAECKAPSIKIGRDTFEQIIRYNSALGVKYLLL
                     TNGMTTYFASFDPNCGTYQFITEIPPYNELFR"
     gene            17867..19273
                     /locus_tag="P5293_01225"
     CDS             17867..19273
                     /locus_tag="P5293_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR48589.1"
                     /translation="MNFLDKKIFSLVAEEAQKLNMKAFVIGGYVRDCFLERKSNDIDI
                     VVEGSGIELAERVAKRLKVKVTVFKNFGTAMLKYDDIEVEFVGARKESYRSDSRKPIV
                     ENGTLEDDQSRRDFTINALAFSLQKEDYGKLIDPFGGVKDLQQGIIRTPLEPDTTYSD
                     DPLRMIRAIRFASQLSFKIVPESIESIKKNKERINILSKERISDELSKIILSPKPSVG
                     FQLLDQTGLLQLILPQLVKLKGTEFVEGVGHKDNFSHTLQVLDNLAQKSDNLWLRWAA
                     LLHDIAKPQTKNYEPGTGWTFHGHEYLGSKMIPGIFQQLKLPLNEKMKYVQKLVLLHL
                     RPIALVQDEVSDSAVRRLLFEAGDDIDDLMLLCEADITSKNRKTVKKHLANFALVREK
                     LVQVEEKDAIRNFKNPISGEYIMEHYGIPPSKEVGIIKEYIKDSILDGIIANNFEEAK
                     MLMEKKAGELGLVCKETK"
     gene            complement(19294..20802)
                     /locus_tag="P5293_01230"
     CDS             complement(19294..20802)
                     /locus_tag="P5293_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48590.1"
                     /translation="MRKKLKNLFIAILMIALWSLMPSCTKDDNPESEEEVVDGVLFNG
                     NQIGSGDQEFEIKSSYTLKKGIYILKGWVYVTDGVTLTIEPGTVIKGDKDTKAALIVE
                     RGGKLIAAGTKNEPIVFTSNQPAGSRKPGDWGGVIVCGKATNNKREMIIEGGPRSSHG
                     GDNDDDNSGVMSYVRIEFAGYPFRTDQEINGLTLGSVGRGTRMDHIQVSYSNDDSYEW
                     FGGTVDAKYLIAYHGWDDDFDSDNGYRGRIQFGLVVRNPRIADKSSSNGLESDNNAEG
                     TTELPLTKPVFSNITFLGPKSQDNLFENTTSYITGGEYYPGNGSKLGQFQGVMQIRRS
                     SNLNCYNSVAAGYPVGLLICNDKGSTTQSYATSGAINLKNLVFADMTILGSDKDASFK
                     DSLSTNASTFDKNATESFSSSFFKAANNLNTVYPTVSDLKFSQPNSLIASPNWGPLSN
                     SPLLNRANLFVDDNLSASFFDRVNFIGAFRSDLLVDNWTAGWANFNPQYTAY"
     gene            complement(20816..>23528)
                     /locus_tag="P5293_01235"
     CDS             complement(20816..>23528)
                     /locus_tag="P5293_01235"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRR48591.1"
                     /translation="NTGLPVIGASVYIDSIKKGGVTDTAGNYVITNIPRGIYNLEISS
                     ISYNSVKVKDLRINAPSVKFDIIMEEASNILNQIVVTSAKRMSSEIAVIQSIKSADIV
                     MSGISSQQISKSQDRSAAEVVKRIPGVSVIDDRYIMVRGLSGRYNNVWINNASVPSTE
                     ADTRAFSFDMMPSSQIESIMIVKSPSAEIPSDFSGGFVKIMTKNMPESNETLLSYGLN
                     FNTSTHFNDHFYNTSSPTDFLGFDSGERAIRKVVPQRLDNNNPSQVDEVTSRGFRNDW
                     RVHEHRPIADQRINLMISRYRFTEKGTKLGITGALNYSYSSHTYSDMLNARFGVYNTV
                     SDEPEYIYKYVDNQYSIDAKLGGMLNITCIKGNNKFEFRNIFNQQGKNRYTIRNGWQN
                     ISALYMQENREYLYSSRTTYSGQFAGSHKIRSSDIDWIAGYSYAGLNQPDRRLINREE
                     NLIYGDKYYGMMAIDQNEITRDFVNLDEHTFSGSLNYTLSLGKGHTIVSQIKAGLFGE
                     YKSRDYNNRQFFYRFNQYNLPENFVYQDVVDEILKPDNYGQNKLYIYEDTDNRNSYKG
                     QNVLTAGYLSYKFSVGLFNLLAGVRIESNRMKLTNYTKIYEFDTADREYNQFDIFPSL
                     NASYNLDTKNIIRLAYGISTNRQEFREVSPSVYYDFNLFSDVKGNTDLKNATIHNLDL
                     RYEYYPSKDEYITFALFYKYFKNPIEWTYLDAGGSYTYTFENALSANNYGAEIDMKKN
                     LDFVGLDNFTLGLNAALISSQINFDSSSMEIDRPMQGQSPYLVNTSLYYDSKKTGISV
                     GVLYNRIGKRIVGIGRVDTGSGASINNDVPDTYELPRDLLDFVLAKRIGKHLEFKANF
                     KDILNQKVVFAQYPRFEDEDGNINKRKQVSKSFRPGTDIYIGLQITF"
BASE COUNT         7530 a         4575 c         4103 g         7320 t
ORIGIN      
        1 ttttgatctg catcaaactt aagagattga atcctaatgt ccataaaatt atcctccttt
       61 ttttgctctt ggatgagcgg ttttaaatac tttttttaac tgttcaaatg aattatgtgt
      121 atacacttgc gtagccgcaa gagatgagtg ccctaaaacc tctttgatac tgtttaaatc
      181 tgcaccattg ttaagcagat gagtagcaat gctgtgtctt aaaacatgag gcgatttctt
      241 gccagaaaat ccctcaactc caagaagctc tctccttaca acattattga caaaagcaag
      301 atacaaaggt ttacccgaat ctgtaacgaa taatggagat atggaagtaa caggaaaatc
      361 cttagaataa caagcaagat agcgctccaa ttcctccctg agtgagtttg ttatgggaat
      421 ttctctctgc ttgtctccct tgccggtgac acgaaaaaga gaacgattca tgtcccaatc
      481 cgatacttta agatttgcca attctgcacg acgcatgcct gtattatata aagttgaaac
      541 tatagccctg ttacggagag atttataatc tttgcatact tccttagaga agtagttttc
      601 catggcatgt tcagtataaa attgtggcag tttctttgac tgtttgggac gatgtaccct
      661 tttcaccgga ttggaatcta atattccgca tttgacaaga tagtttgaaa agctgcttaa
      721 agctgagagt ttcaagttca tagttctggg ggatattcca gattcaaggc cggctgcaat
      781 aaaacctctt attagattgg gctttaaaac atccacactc tcttcctccc catcggaagc
      841 aaattcgaaa aattctctaa tatactcacc atatatggct tgcgtccgat aggagtatct
      901 tttttgcgtt ttgagatatt ctataaactc tgcaaccatc cctgttttct ttcgacacta
      961 atataaataa aaaaaatgag ggtgccaaaa ttattgacac tcccattatc aattatcaag
     1021 agctatgtca ctcctcgttt ctgcgaagtg actgtatata gattgccttt ttaatctcgt
     1081 cacgcctttt aaccgatggc tttacataag cctttctgtt tctgagttca cgaattactc
     1141 cagtcttttc aaatttacgc ttgaacttct tcagagcacg ttctatgttc tcaccctctt
     1201 taatagggac tattatcata aagaaaaaca cctccttttt ttgagtttgc aaaagtatgg
     1261 attattttgg taaaagccaa cttactgggc atttttttgt attttcgcat aaaatataaa
     1321 gaaaatggaa cttgaagaat tcagaaaagc tattgaaaaa ggaatacctg atacattgcc
     1381 tgaaccaaaa ccttatgaca ataatataaa tcacgctccc aagagaaaag atattcttac
     1441 aaatgaagaa aaagaacttg ccttaagaaa cgctctaaga tattttccca aaaaacatca
     1501 taagattctg gctcctgaat ttgcccgcga actacaagag tatggcagaa tatatatgta
     1561 tcgcttccgc ccggattaca aaatttttgc acgaaatatt gacgattatc ctcataaatc
     1621 cagacaggct gctgcaataa tgatgatgat cagtaacaat ctcgactgtg cagttgcaca
     1681 acatccacat gaacttattg tctacggtgg taatggtgct gcatttcaaa actgggcaca
     1741 atatctttta accatgcagt atctgtcaca aatgaccgac gaacaaaccc tcgtactcaa
     1801 ttccggccat ccgctcgggc tttttccatc tcacaaggat gctccacgac ttgttattac
     1861 aaacggcatg gtgataccaa attattcatc aaaagatcat tgggaaaaat ttaatgctct
     1921 cggtgtctca caatacggac aaatgacggc aggatcgttt atgtatattg gacctcaggg
     1981 aattgttcac ggtacaacta tcacagtcat taatgctgca agaatgcatt ccaaaaaggg
     2041 agatgaaggt tttaaaggga aactgtttgt gagctccggc ttgggaggaa tgtccggagc
     2101 acagccaaaa gcagctgtaa ttgccggtgt cataggagtc attgcagaaa ttaaccccaa
     2161 agcagtacag acaagatatt cccagggttg ggttgatgaa gtatttactg accttgacct
     2221 tcttgtagag agagtaataa aagctaaaaa caacaaagag gctgtttcta ttgcttacca
     2281 gggtaatatc gtagatttat gggagaaatt tatcgaagcc ggtgtggatg tagatctggg
     2341 aagtgatcaa acttcacttc ataacccttg gtcaggtgga tattatcctg ttggagtttc
     2401 atttgaagaa tccaacagat taatggcaga ggaaccagac aagtttaagg cactcgttca
     2461 ggaatcatta agaagacaag tatcggcaat taacaaactt tctaaaaaag gcatgtattt
     2521 ttttgactat ggaaatgcct tccttctcga agcctcaaga gctggggcag atgttttgta
     2581 tcatgacggg aaatttaaat atccatctta tgtgcaagat attatgggcc ctctctattt
     2641 tgattatgga tttggcccat tcagatggat ttgcacatca tgcaaaccag gagacttaag
     2701 agttactgat gaaatagctg caaaggttat tgaagaaatc ataaatcatg cgcctgaaga
     2761 gattaaaatt caactatctg acaatttaca ctggataaaa gaggctgaat ccaacaaact
     2821 tgtcgttggt tcacaagccc gcattcttta tgcagattgc gaaggcagga tcaaaattgc
     2881 tcttgcaatg aacaaagcca taagagaagg aagaatatct gcaccagtag tgcttggcag
     2941 agatcatcat gatgtttcag gaacagactc tcctttcaga gagacatcaa acatttatga
     3001 cggttcaaac tttacagccg atatggctgt tcagaatgtt ataggagatt ctttccgtgg
     3061 agctacttgg atatcaatac ataatggagg cggagttggc tggggagagg taataaacgg
     3121 aggattcgga ttggttattg atggctccga cgaagcagaa aaaaagatca aagagatgat
     3181 cctttgggat gtcaacaacg gcattacccg acgcgcatgg gcaagaaaca gtggagctat
     3241 tttcgccatt aaaagagaga tggataaaac tccccttttg caagttactt tgccaaatat
     3301 ggcagatgac gatctaatca gcgagactgc gaaacatcat ttttacggga aatcccaaat
     3361 ctgaagcgta gtctttaagt tttaaaacag tattttgttc tacatcttta tctctggaaa
     3421 gtatccaaag cagatctcca tcaggagagc caacaagagc tatagaatag tcattatcga
     3481 ttaataatac ccagtaatct tcggtaaaag gccaattata tgagactttc atctttgaag
     3541 gattcctcct gtctggaatc caacctttgc ctttccactg cttaattcta gttttatcgc
     3601 ttataagacg accttcatta acaattgtaa aagaaccatc ctccatcaaa gttaacagag
     3661 atgtacactc ctcaagtcct tcctggattt tattggggag tctttctatt tcataccatc
     3721 tacccatata acgctctatc tcaaatggtt tagccgtttc tgtgacaaca taagtgggag
     3781 aacatgaagc agctgcaaat aaaaaaatgg ctgaaatcaa atatttcatg ctaagtctga
     3841 atttgagtag agttaaatgt ttcaaattct gtaccaagtg tatcatttat tacactttgt
     3901 ttaatgatat gtcctcattt ttgtaaaaaa tacagaatat gaaaagaaat ataatcaaaa
     3961 ttgacgaaaa gctttgtaat ggatgcggaa gttgcgtaca aggctgtaac gaaggagcga
     4021 tacagcttat caatggaaaa gcagtaatga taagcgaact ttattgcgat ggacttggcg
     4081 catgcatagg agaatgtcct gaaggagcaa ttgaacttgt tgaaaaagaa gcagagccat
     4141 atgatgaaat tgcagtaatg aagagaatcg caataaacgg tgaagaagca gtaattgcac
     4201 acctaaaaca tcttcaatac catgacgaaa cagaatggtt aaaccagggg ataaactggt
     4261 gcaaagaaaa taattatctt atcgatctct caaaacttga taattataaa ccaagaatgg
     4321 cttgcggatg ttcaggatcc cttgaaaaag actttaaaca acccgaatcc tcaaataata
     4381 gggacaatat gtcatctaac aatatgacag aattaaagca gtggccggta caacttcatc
     4441 tcctcaatcc acaagcatca ttttttcatg aggcagatgt acttcttgct tctgactgtt
     4501 ctgcatacac tttcagcaat tttcatgata aatttctaaa aaacaagtct ttagcaatag
     4561 cgtgccctaa actggacaat aatattcaaa gttatattga caaaatatcg gagatgatct
     4621 cagtttctaa aattaatacc cttactgtgt taattatgga agtaccttgt tgcagcggtt
     4681 tacttaaaat agcagtgagc gcaagagaaa aatcaggtaa aaatattcct ataaaggcag
     4741 ttacactatc ggtcagagga gatattatag ataacaagtg ggtctaaaac tcctgtcaaa
     4801 gaagttgaga acttttatac ttatttataa acaaggaaag aggctcggga agttcaagtt
     4861 catctttgag aataagattc aatgtttgtc ttccttcatc agtcaggtca aaatacattt
     4921 gtcttttatc cctctcaccc attgctctgg taataagacc cttttcttcc acagagcgaa
     4981 ttagtttaga ggtatttgag caactcagag atagcttgtc ggcaatttcc ccagaggata
     5041 actgtccatc tttcagagaa cataaaagca ttccttcatt taaacagatc ccatacatat
     5101 tgtgaaaact ctcttcataa gaacaaagtg ctctacatag atctctgata gcgcataacc
     5161 tttccatctt caaaaatgtc gtttaaaagc ttcgcaaaaa taatgtaatt atctatttgt
     5221 tgagcaaaac ctcatcgata gcttttttca aatttgcttt tggaagagca ccttgagcaa
     5281 cttgcggatt tcccttcata ggtataaaca acaatgtagg aatacttctt attccaaaag
     5341 cagcagctaa ctcctgctct gcatcagtat ccactttata aatatatatt tgaccttcgt
     5401 attcagctgc aagttcctct aaaacaggag caattgattt acatgggcca caccagtctg
     5461 cataaaagtc aacaagtgca ggtttatcac ctaaatactt ccactcatct ggattgtttt
     5521 caaagtcaac taccttattt aaaaattctt ctttcgtcaa atgtattgtc ttcattcttt
     5581 tttcattaat attattaata ctactattat tttgaattct attgttttgt gatatcccat
     5641 ttttagcaga tgcactgtta ccgttattgg aagtgcacga aattattaaa atgggaaaca
     5701 ttaaaaaaat cagatatttt gtaactttac acattgttta aactttatgt tattttccgc
     5761 aaagttaatt attgtttcct ttaaaacaaa tttctttagg aaaattttta ttttagatta
     5821 tattttaatt catttacaaa tattttaaat ttatgaaata cataattatc ggaggagttg
     5881 caggaggagc aacagctgct gctaaaataa gaagagaaga tgagcatgca gaaattataa
     5941 tgtttgagag aggggaatat atctcttacg caaactgcgg attgccttac tatataggag
     6001 gaaaaattga aaaaagagaa gatctcatcg tccaagggac aacaacattt tcaaaccgtt
     6061 atgacataga tgttagaacg ttcaatgaaa ttacatcgat tgacacctct acaaagaaag
     6121 tcactgtcag aaacgttcag aacggcaaga tctattttga gagttatgac aaactgttac
     6181 tctcgccggg agctatgcca ttcaagccct cattacctgg aattgacgat ccaaggatac
     6241 tgacagtcag gactatccct gatacagaca agattaagag tttaacagaa aagactaaaa
     6301 aggggaaagc tgtaatcgta ggaggaggat ttataggact tgaaatggca gagaatctca
     6361 aagctctcgg ctttatggtt acaattgtag aaatgatacc acaggttatg acccaactcg
     6421 accctgttat ggcctctatt gtgcataatg agcttagaga aaatggtgta gaattaattt
     6481 tgggagaagc tgttatggcg ttccgtccta caagtgaaaa gctatatgtt caactcaaaa
     6541 gtggaagaga aatatcggct gatctggtaa tattgagcat tggagtaaaa gccgaaacat
     6601 ctcttgccag agaagcaggg cttaaaatag gaataaccgg tggaattgag gtaaatgatt
     6661 atcttcagac ctccaacaaa gacatctatg ctaccggtga cgctgttgaa attattaatc
     6721 ccataaccgg agatccttcg ctgacaccac ttgcagggcc tgcaaacaaa caggcaagaa
     6781 ttgcagcaac taatctggtt cacggtaata tttgcagata taagggatcg ataggtacag
     6841 gaatagcaaa gattttcagg attacggcag cagtgacagg agtcaatgaa aaaacactta
     6901 acaagaaggg aatcaaattt atttcaacta ttacacattc gggttctcat gcagggtatt
     6961 atcctggaag cgaatctctt tcaataaaga ttctctattc acccaaaaac ggaaagttgt
     7021 ttggagggca agtcgttggt tatggaggag ttgataaacg aatcgatatt attgcgacta
     7081 taataggtca gaatggaacc gtttacgatc ttatggaaat agaacacgct tacgcacctc
     7141 ctttctcctc agcaaaagat ccagtaaata tggcaggatt tgtagctgat aatgtcataa
     7201 atggaaaagt taaaatcgta acttgggata ttataaataa ttttgatttt ggcaacgact
     7261 atctcctaga cgttagaagc cctgaagaat gtaagcaggg aatgataaaa ggagctgtaa
     7321 atattccttt ggaagagctt cgtttaaggc ttgacgaaat accttcagac aaaagaataa
     7381 tagtatactg tgccataggg atgagaggat atcttgcttc aagaatttta ttgcaaaatg
     7441 gctacggtaa tacttataac cttacaggtg gatacaagac ttatctcttt gctcataagg
     7501 atcaggaaat aatgactaaa ttaggtgata caaaaaataa atcatgaagc atcttgctaa
     7561 atttttactg aagattatgg gatggaaagc gttgacccct ccggttcctg aaccaaaatg
     7621 cattatactg ggtgtacctc acacttctgt ctgggatttt gtaatatcat atctttatta
     7681 taaatcattt ggtgcaactg cttacatcat gataaaaaag gaatttttcc ggggtttttt
     7741 aggaccaatt cttaaaagta tgggtggaat tccggttgac agaaagcacc cgtcaactat
     7801 taccagacaa gttatagagg cattcaacaa tactgatcat cttcaccttg ctattgctcc
     7861 tgaaggaaca agagataaag tcgaaaaatg gaaaaccgga tttcacatta ttgcaaaagc
     7921 agcaaatgtc cccgtttatc ttggttattt tgattgggga aaaaaagaaa taagcagagg
     7981 tattaagttt gaattatcag acaatgtcaa cgaagacctg agaaagattc gtcaatatta
     8041 taaagacttg ggtgttaagg gtaaacatcc tgaaaacttt accacaggaa gtgatctgga
     8101 ttaaatctct taaaaattta aatcaatcct tccgacataa acacctgctg tccccatttg
     8161 agtaaccatt actcctcttc cgcttctgtt ttttacaaca gtaggttcct ccagaaaagt
     8221 gtgagtgtga ccaccaataa taatatcaat gtcccggctc gactttgcaa gatcaatatc
     8281 tgaaggagag cggctatctt catcgtgatt atacccaagg tgagtcaaag ctattatcag
     8341 atcacatcct tgtttatatt taagatattt tgccatatcg ttggcacatc ttatgactcc
     8401 caaatattta attccggaac gagccgattc tgcaacatat ccatttaggt caagcaacag
     8461 gccaaagata ccaattttct ttcctccctt ctcaattatt acatatggct taactaaatc
     8521 tttcagcggg ctcttggaaa aatcatagtt tgcacaaagt gtaggatact ttgccttaga
     8581 gagcctctct gccaattggg cttctccatt gtcaaattcg tgattgccca ggcatactgc
     8641 atcatatttc attgcattgt acagcataac ctccatctct cctttaaaca aagtaaaata
     8701 aggagtgccc tgattatagt cgcctgcatc caataataaa acattatctt ctcgctctct
     8761 aacattctga atatagttcg cacgtctctg ggctccaccc attcctttat tcctgccacc
     8821 agagagaggt tctacattac tgtgaacgtc attggtatgc agaatcacta tttcttgtgc
     8881 aattgtgtta tacggaagaa aaagcgcagt tattaataat attaaaataa ctttcaatct
     8941 cattactgtg caatttttat tctgtttgta attttggaag atattttctg attattagcc
     9001 gttaaggact ctattttctc aagaattaca tctcttagaa gcataccggt ctgctcaact
     9061 ttaacagctt ctttaagagg ataaagattg tcgccaccgg tcattagaaa atcaagagta
     9121 gcaaccctat aggtctttct atcatcaatc tgaaccccat taattaaagc ataatttagc
     9181 ttaccagact ttacggaaag aaaaacattc gaagataaag gctcgatact tgctgccaga
     9241 atattaaaaa gttttttgac agagctccct tttaaatcaa taatcacaag ataattatcg
     9301 aaaggatatg ttgagtagat atcatatcta cgcacttcgc ctgcaggaat agaagctctc
     9361 aggcctccaa agttagtcaa cgcaaagtca acattatttt cgcttttcct ttgagcaatt
     9421 tctaacattg catctactgt gaagtttgaa agaagacttt ccggtcttcc agatctcatc
     9481 tcttgctcag aaattcccac tacttgttgc atttgactat cgacctgcgg tttataaaaa
     9541 tctataattc cttttacgac cagctcttca ggaaaattcc atgtactgtc cattaaaact
     9601 cttgttgctt tccactgaaa agtcgtttcc tgagcgaaag tatttaaggc gaaaaaaatg
     9661 gatagtgaaa atagtattgc ctttttaaac attatgctat taaattaaaa cttcaaccat
     9721 ttcacaagat ctttccatgt ggctttcttg ccatatgata gaatacccac tctgtatatc
     9781 ttggcagaga gataagtcat aaatataaaa gtagctaaca ataacataat agaaagaatc
     9841 atctgccata aaggtactcc aaaaggcact cttgccatca taaccatagg agaggtaaat
     9901 ggtataattg aaccccagaa tgataaactg ctgtcgggaa atctaaaggc atgaatcatt
     9961 aaaaatagac caattatcaa aggcaaggtt attggaattg taagttgttg ggtgtctgtc
    10021 tcattatcta cagcagagcc aacggcagca aacattgagg cataaagcaa gtatccaaga
    10081 ataaaatatg caagaaatga caaaattata tatccaaaat taattcctgc aagagcagtc
    10141 agcatttctc ctacttttcc gccagaagca gaaaaacctt caggcaatgc tccaggaaca
    10201 cttccgcctg tggaggcaag atctacgccc ataaaagcct gagctccaaa aataattgca
    10261 aacgttaaag ctacccaaat gagaaattga agcaatgcaa ctgcccctac acctataatt
    10321 ttacccaaca tcaattcaaa aggcttgaca gaagagatga ttacttcaac taccctgttg
    10381 gatttctcat caataactcc ccgcataacc atggatccaa acatgaagat aaacatgtaa
    10441 attaaaaaac tagctaaata tgagatagcc atatttatct ctaccattcc tgacttttcc
    10501 ttcccctctt cgctaacagt atatgtttta acatctattt ttgtattaat gtctttgata
    10561 atggaatcga gattctggat attgtaagct gccagttttt tatctctgat tcctctttcg
    10621 acacattttt taatctcttc cttagtatca atatttatct gtttgttaga gtatgttgaa
    10681 actgaaacat tataagctga atccatagga gatatttcaa ctatggcata aagatcagaa
    10741 gttgcgagat ccttcttaat gctttgaata tccgcatttg gatcaaacct gtatacatag
    10801 tcctcatttg aaacaaaata cttttcacac tctctcgacc tgtccactac aagtatcttc
    10861 tgttctcttt cactaggaga aaaagacatt attatcgtag gcataacaat aagcaaagcc
    10921 atgaatattg gagtaagaat tgtgagcaga atgaaagatt ttttcttgac tcttacagag
    10981 aactctctag aaataattaa aaaaagtttg tttttatcca tagcctgcaa attaacttac
    11041 cagtttaata aaaatatcat tcattctcgg aacaagttcc ctatattcaa ctatgtcgat
    11101 gttctgttta agaagctcca taagtacctg tgaagaagat gcttcccgat caacttcaat
    11161 aatcacacga tatccatcct tatatatatc gttagaaaca actctgtaaa aaccattgct
    11221 gacaatctct ctaatttcag aagaacggta tattatctca acctcatttt tgccataatt
    11281 ttttcttatc tcatcaacac ctccagaaac aacaagtttt gctttgttaa tcaatgctat
    11341 attgtcgcac agttcctcaa ctgactccat attgtgcgtt gaaagaatca cagaagtccc
    11401 gttatccttc attttaagta tttcgttcct tataagctga acattaaccg gatcaaaacc
    11461 actgaaaggt tcatcaagga ttaaaagtga aggtttatga agtacagttg tgataaactg
    11521 tactttctgt gccattccct tggaaagttc ttcaactttt ttgttccacc aactctgaat
    11581 gccaaacttt ataaaccact ctttgagctc tttcacggct tcagcgtggc tcatcccttt
    11641 cagccttgca aaatagacag actgctcacc aactttcatc tttttataca aacccctctc
    11701 ttcgggcaga taccctattc tggtcacatc tgaaattgtt ataggccttt cgcagaacct
    11761 aacttcacct gaatcaggaa ttgtaatctg tgtaataatc ctgattaatg tagttttgcc
    11821 cgccccgtta ggtccaagca atccaaatat ggtgccttga ggcacttcaa tgctcacacc
    11881 atccaatgcc gtaaattgat tgaatttttt tacgacactg ttacagctaa taacattcat
    11941 aaattgttag aaatataagt aaaaaataaa tatttgtaat attggcaaag ataaaaacaa
    12001 tatttactga aaataaaatt ttaaaatatt atatagttca tttttaataa tattttctac
    12061 aacaagagaa gcaagattta ttatgtcgtt acttctcgaa tctctaaaac gaataataga
    12121 cctagccaaa tatccgccaa tgtatggatt agttgaaagc tgttcataag ttgcttcgtt
    12181 taagttaatt ttttgaattt tatttgtgtc aacagtgatt ttatcagaaa acatagaata
    12241 acgttcctca tcaattccga aaatatccat tagctgacgt ttgtccgcaa aaccgccaag
    12301 cttgtctctg tattggatta ttttttttgc ataataaggc ccgattccgg gtaatgtcac
    12361 aagagtagca ctgtcagcgc tgttgagttc aatagatttt aaaatcctct tctctgattt
    12421 aagattatca gttctgacat ttgtcatatt ttcaagtgca atatcccggt aactcttcca
    12481 tttagcacta ctatcggcca acttggcact ttttgctggt aattgagcag tagtgtttgg
    12541 aatgcgaatt ttcggccttt cgccataaga tttcaagtgt tttgcactgt aactcgcaga
    12601 atctgactct gccactttct cgggttgtaa tgatttgttt aatgaattat cctcttgctt
    12661 gttaaacaaa aaaagtatca actgaatcgt caacacaaca ataatgagtg tcattactcc
    12721 agacgctcga gtttttgaaa tttttattct ccacatatct ctctcctcca ttaataatgc
    12781 ttaatcaggc tttcctgcaa ttgttataac tatctccccc tttggttcat gctccaagta
    12841 ccagtcgcga agttcaatca gtgttcccag tctgttctcc tcatgaactt tagtcagttc
    12901 gcgtgacaca caagcttttc tatcttcacc aaaatattct acaagttgtg acaaacactt
    12961 cacaagtcta taaggagatt cgtataaaac aattgttcta gtctcctgac ctaactccct
    13021 taacctcgaa atacgccctt ttttgatagg gagaaaccct tcaaaacaaa atctgtcaca
    13081 gggcaaacca gaattgacca aagcaggtat taacgctacg gctccgggaa gtgtttcaac
    13141 ctctattcct ttatccctgc ataatctcac cagtaaaaaa cctggatcgg aaatcccggg
    13201 cgttcctgca tccgaaatca aagctatatt ctcacctcca agaatcctgc tgcatataaa
    13261 atcaacattt ttatgttcat tgtgcatgtg atgggactca agtcttgttg caataccaaa
    13321 gtgtttaagt aaatgtgaac tagttctcgt atcttcggca agaacagtat cgacttcttt
    13381 tagtactctg agagccctta gagtaatatc ctcaagattt cctataggcg taggcactat
    13441 gaatagtttt gccattaaac tttcttctaa tgttcatata aaatctgtag actgaagaag
    13501 aattttcatc ccaatctgga aatgcatttt tagtataatc catgacctct tcaagcgatg
    13561 tcatttcatt gattttctgc agacttagcc tgtattgctg ttcatctccg ttaaaaagtt
    13621 cctttatata caacaattta tcattcagag taataccatc agtaatgtca gttatttttg
    13681 gtccaggaat atctgtcatc caggctggtt ttttgcctct tgcaacatcg agaattgact
    13741 ctccactatc cggaaaaagt tcaaattcct cctcatcatc gattgccaca gtctctttct
    13801 cttttttatg atcgttaacg ctgaaagatg tcaatgaaca atcattctct gaaatatccg
    13861 ttttactttt tgacttaaga cttttcaatt cagaaagcga tagttctatc tgttcaagat
    13921 aaagtgaatt gattttacat ttaatacttt ctgccgcaag aattaactct tcccatctgg
    13981 ccgggcctat tttatcatcc tccttcaacg cctcaatatc ctgtgaaatc tgatcaagaa
    14041 taatccctat ttcctcaatt ttgctattgt ttgacatccg aactttttac ctttgtataa
    14101 gcacaaaaat agacaaaatg tttctggaaa acacaaaaaa atccggttgg attgagataa
    14161 tctgtgggtc aatgttttcg ggtaagacag aagagcttat cagacgattg aggagagcaa
    14221 aatttgcaaa tcttaaagtc gaaatattta aacccaaaat tgatactaga tactccgaga
    14281 gcgaggtagt atcacatgat gcaaatgcaa tccggtctac agctgtagat tctccattaa
    14341 gtatactact gtatgcgtca gatgttgacg tagtaggaat agatgaggca caattttttg
    14401 acagttctat tgtggatgtt tgccagcaac ttgcctcaaa cggcattaga gtgattgttg
    14461 caggactgga tatggactat ctgggaaatc cttttggttc aatgccggaa cttatggctg
    14521 ttgcagaata tgttacaaaa gttcatgcaa tttgtgtaaa atgcggagat cttgcacact
    14581 attcacacag attaacaaaa aacgacaaac tggttatgat tggtgaaaaa gacacctacg
    14641 aaccagtttg tcgtcattgt tttaacgatc tcagaaagat cgattaaatt actttactgc
    14701 tgtaccggag cagctttgcg agttagattt gccttaccgc tatgagtagt gttggcagca
    14761 gcattaactg ctgtgaaggt aatcttaagt ccttctggtt ctgcaacata agtcattgaa
    14821 ccgctaagat ccataggctc tactccctgc attgaacctg taaaggtaac aacgtcctca
    14881 gcaatagtag ctacgccacc aacattgata gaggtagagt cattgatttt aataacgcct
    14941 gtgaaagttg agtcagcaaa gtattcgaag tcgataggag caacgaattc ttgagcaact
    15001 tgattaccag ttgaatcaac aactgtaatt gagattgaat cagtgccggt ccattgacca
    15061 accaattgct ctgaaacagg cttagctttc tgaccgcatg aagcgagtgc tacaactgat
    15121 gcagcaacag caaaaaatgt ttttaatttc attttattat tgttttaggt atgtctttcg
    15181 actgcaaatg tagaaagttt ttcaatacaa aatttttttt taaaacaatt tttaaatttg
    15241 cttcaaataa tttaaatcaa atgacttact caaaacttgc aattaacaaa gaaaacttaa
    15301 ggcacaatta tgactatttt cgctcaaaac ttgctcaatc aactaaatta cttgtacttg
    15361 taaaagcaaa tgcttacggt cacggagatg ttgaatgttc aaaactatta gaagaatttg
    15421 gtacagatta tcttggagta gctactgtag aagagggcaa aagattaaaa aatgaaggca
    15481 ttaagttgcc aattattgtt ctaacccctg gtaatgataa ttttgaagag ataataaaat
    15541 attcacttga accaagtata gtcaatttcg attccctcca atcatttgtt acatcactta
    15601 aaacactgtc taaaaccaag tatcctgcac atattaaatt agatacaggg atgcatcggg
    15661 taggatttgt taaatcagaa attgatcttc tttcaaattt attaaagcaa aatccatata
    15721 tcgaagtaaa atcagtcttt tctcaccttg cagctgctga tgaagctgtt cacgacgaat
    15781 ttacaagatc acagattgag ctgtttgaag agatatatgc taacctgagt gacaaaattg
    15841 aatccaaacc tatcaaacat attctcaatt cagcaggaat agagaggttt tcatatgccc
    15901 agtttgatat ggcccgttta ggaataggca tttacggtat aagctacgtt aatccggact
    15961 gtcttaaacc agttggattt ctcaaagcac caatcattca cattcgcgaa atttcagaag
    16021 gtactgttgg atatggaaga catggtgatt taaatggaaa agccaaaaaa attgcaacga
    16081 ttcctctcgg ttacgctgac ggtgttaaca gacacctggg tagaggttcg gcctcatttt
    16141 ctctaaatgg tataagagta cctacaatag gaaatatatg catggatgct actatgctcg
    16201 atataacagg agttgatgcg aaattagggg acatagttac gatttttgga gataacccaa
    16261 aagcccggga aattgctgat attctcaaga caatccctta tgaaatcttt acctccatct
    16321 ctgccagagt aaagagagtt gtagagtaat gttcttaatt tgtaattctg aagaccaact
    16381 cggtaagtaa ttccttatcg gttgcttcgc ctctttcatt gcccttactt actgaatcac
    16441 actttcgtat caaagaaatt atctccatac acttcgcata tgaataatta tctgatgcaa
    16501 ttacatactc ctgcagatga taagtcttaa tgcctaatgc tgtgcaaatt gtattattgt
    16561 caactgaact attactactg acaagcgagt gatatttaag aactttcgca aaatgagaaa
    16621 aaagcacagc aagtgtaact acaagaggat aaagttttgg agagtcccca aaatgtgaag
    16681 ctattttaaa agcctttgct ctgttcttaa aagaaagagc ttttgtaagt tcaaaaagat
    16741 tgtattctct actgattccg atattatctt ctatatgccg agtagtaatc aaagattccg
    16801 atgcatcaag atttgtaatt aatttattta gttcgtgagc taattttctc aattcagtac
    16861 cacagtaatc agccataagc atggaagcat ctgcatcaat ttttttaccg gcactgttaa
    16921 catagtcgtc tatccagtag acaagcttgt tttctttgag aggtaccgtc tcaagcaata
    16981 ctccgttttc aagtgctttt ttgtaaagaa tagtccttcc atctatagtt ttgccattca
    17041 ttgccaaaac aagaagcgta gttgaaagag gattactaaa atatttctca aaagattcca
    17101 ttttatcaag aaattgtgcc tcctttacta taacaagctg tctggcagcc atcatagggt
    17161 atttcataga ggcctccacc acatcttttg aggaaacatc catcccatac aagattaaaa
    17221 gattgaaatc gtgctcttcc ggcaatattg ctctgttaat aatttcttcg atcaaaacat
    17281 ctgcgtaata aggttcatct cccattaaaa gataaaaagg ggcaaattca cctgccttaa
    17341 tatttgcaag aatctttcta aattcctttt cagtatcctt ttctttgcct gccactatat
    17401 ttttctttta tcccggctaa attatgaaaa aatgtaaacc ttacaaattt tatatctttg
    17461 tacaaatttt cacttgaaat gaaggaacag gtttttgacc ctgttagaag aaaaaaagtg
    17521 gctcttacac cagaagagtc tgtcagacaa cagttgatat attatttaag caataccctg
    17581 ggatatccca tttcaagaat ggtctgcgaa tattcggtcg agattaatgg gcttaaatac
    17641 cgtggagatc tggtaatcat ggacaaagaa cttaatcctt ttttcctggc tgaatgtaag
    17701 gcaccctcca tcaaaatcgg gcgagatact tttgaacaaa ttatcagata caatagtgca
    17761 ttaggtgtca aatatctgct tttaacaaac ggaatgacaa catattttgc atctttcgac
    17821 ccaaattgtg ggacttatca gtttattact gaaatacctc cttataatga actttttaga
    17881 taagaaaata ttttctttag tcgccgaaga ggcacagaaa ctcaacatga aagccttcgt
    17941 tattggagga tatgtaagag attgctttct cgaaagaaaa agcaatgata ttgatatcgt
    18001 agttgaaggc agtgggatag aactggctga aagagtagct aaaagactta aagttaaggt
    18061 tacagtcttt aaaaactttg gcacagcaat gcttaagtat gatgatattg aagttgagtt
    18121 cgtcggagca cgcaaagagt cttacagatc cgattcaaga aagccaatag tagaaaacgg
    18181 gacacttgaa gatgatcagt caagaagaga ttttaccatc aacgccttgg cattcagcct
    18241 tcaaaaagaa gactatggca aattaattga cccatttgga ggagtaaaag atcttcaaca
    18301 aggaattatt cgcactcccc tcgaacctga tactacttac agcgatgatc ctcttagaat
    18361 gattcgtgct atccgctttg cctcacaact ctcatttaaa atagtccctg aatcaataga
    18421 atcgataaaa aagaacaaag aacgaataaa cattctgtct aaagaaagaa tcagtgatga
    18481 actctccaaa ataatattgt cgccaaagcc gtctgtcgga tttcaacttc ttgaccagac
    18541 aggattgtta caactaattc taccccagct tgttaaactc aagggtactg aatttgtgga
    18601 aggagtagga cacaaggata acttctcaca caccttgcag gtacttgaca atctggcgca
    18661 aaaaagtgat aatctttggc ttagatgggc agcattgtta cacgatattg caaaaccaca
    18721 aactaagaat tatgaacccg gaacaggctg gacttttcat ggtcacgaat atttgggaag
    18781 caaaatgatt cccggtattt ttcagcaact taagctaccg cttaatgaga agatgaaata
    18841 tgttcagaag ctggttttgc ttcatcttag gccaatagca cttgtacagg acgaagtgag
    18901 tgattctgct gtgagacgtc ttctttttga ggcaggagac gatattgacg acttgatgct
    18961 tctatgcgaa gcagacatta cctccaaaaa ccgcaaaaca gtcaaaaagc acttggcaaa
    19021 ctttgccctt gtcagagaga aacttgtaca agttgaagaa aaagatgcta taagaaactt
    19081 caaaaatcca atttccggcg agtatattat ggaacactac ggtattcctc cttctaaaga
    19141 ggtaggaatt atcaaggaat atatcaagga ttctatactt gatggcataa ttgcaaacaa
    19201 ttttgaagag gctaaaatgt taatggaaaa aaaggccggt gaactcggcc ttgtatgtaa
    19261 ggaaacaaaa taagtttata taccggttgc agcttagtaa gctgtatatt gtggattaaa
    19321 attagcccaa cctgctgtcc agttatcgac aagtaaatct gatctaaaag cccctatgaa
    19381 attgactcta tcaaaaaatg aagcagagag attatcatca acaaacaaat tcgctctatt
    19441 gagcaacggg ctgtttgata acggccccca gttgggtgaa gctatcaaac tgtttggctg
    19501 tgaaaatttt aagtctgaaa cagtgggata aactgtattc aaattatttg cagccttaaa
    19561 aaaagatgaa gaaaaggatt cggttgcatt tttatcgaat gttgacgcat tggttgaaag
    19621 tgagtcctta aaggaggcat ctttgtcact acccaagatt gtcatatctg caaaaaccaa
    19681 atttttcaag tttattgccc ctgatgttgc ataagattgc gtagttgacc ccttatcatt
    19741 acaaatgagc agaccaacag gatagccggc tgcaacggaa ttgtagcaat tcagattaga
    19801 acttcttctt atctgcatca ctccctgaaa ctgtcccaac tttgatccat tcccgggata
    19861 atattctcct cctgttatat atgaagttgt gttttcaaaa aggttatcct gagactttgg
    19921 ccctaaaaag gtaatattag aaaagactgg ttttgtcaat ggaagttcgg ttgttccctc
    19981 agcattattg tcagattcaa ggccattgga tgatgatttg tctgcaatcc tcggattacg
    20041 gacaaccagt ccaaactgaa ttctccctct atagccattg tctgaatcaa aatcgtcatc
    20101 ccatccatga taagctatca gatacttggc atctactgta ccaccgaacc actcgtatga
    20161 atcgtcattt gagtatgaca cttgaatatg atccattctg gtgccccggc cgactgaacc
    20221 aagtgtcaat ccgttgattt cctggtcagt cctaaacgga taccctgcaa actcaattct
    20281 tacataggac ataactcctg aattgtcatc atcattatct cctccatgac ttgaacgagg
    20341 tcctccttca ataatcatct ctcttttgtt atttgtggcc ttaccacata caataacacc
    20401 tccccagtct ccaggttttc tagatcctgc cggctggttg gaagtaaaga caattggctc
    20461 gtttttagtg cctgctgcta tcaattttcc tcctctttca actatcaaag cagccttagt
    20521 atctttgtct ccctttatta cagtaccagg ctctatagtc aaagtaacgc cgtctgttac
    20581 atatacccaa cccttgagaa tataaattcc ctttttaagg gtataggaac tttttatttc
    20641 aaactcctga tctccactac caatttgatt gccattaaat aaaacaccgt caactacttc
    20701 ttcctcactt tctggattgt cgtcttttgt gcatgatggc attaatgacc ataacgcaat
    20761 cattaagatt gcaataaata aattttttaa tttttttctc atatttataa aattattaga
    20821 aagttatctg taaaccaata tatatatccg ttcccggcct gaaacttttg cttacctgtt
    20881 ttctcttatt aatattgcca tcttcatcct caaatctcgg atattgtgca aatacaactt
    20941 tctggtttaa aatatccttg aaatttgctt taaattcaag atgtttccct atacgttttg
    21001 ccagaacaaa atccaaaaga tctcgtggca actcataggt gtcagggaca tcgttgttta
    21061 tactggcgcc tgatcctgtg tctaccctgc ctataccaac gattcttttc cctatcctgt
    21121 tataaagaac acccacacta atccctgtct ttttggaatc ataataaagt gaagtgttga
    21181 cgagatatgg agattgaccc tgcataggtc tgtctatctc cattgaagaa ctgtcgaaat
    21241 taatttgact tgaaatcagt gcggcattca ggcctaatgt aaaattatcc aacccaacaa
    21301 aatcaaggtt cttcttcata tcaatctctg ctccgtaatt atttgcagaa agtgcgtttt
    21361 caaaagtata tgtataagac ccacctgcat cgagatatgt ccattcgatt ggatttttga
    21421 aatatttata gaataatgca aacgtaatat attcatcttt tgaaggatag tactcatatc
    21481 ttagatcaag attgtgaatg gtggcatttt tcaaatctgt atttccctta acatcagaaa
    21541 agagattgaa atcgtaatag actgatggag aaacttctct aaattcctgt ctgtttgtcg
    21601 agatgccata tgccaatcta attatatttt tggtgtcaag attgtagctc gcatttaatg
    21661 atggaaaaat atcaaactga ttatactctc tgtctgcagt gtcaaattca tatatcttgg
    21721 tatagttcgt cagtttcatg cgattgcttt caatccttac tcctgccaac aggttgaata
    21781 atcctacact aaacttgtaa gacagatatc ctgcagtgag gacattctga cccttataac
    21841 tgtttctgtt atcagtgtct tcataaatat aaagtttatt ttgaccataa ttgtcaggtt
    21901 ttaatatctc atcgactaca tcttgataaa cgaaattttc aggtaaatta tactgattaa
    21961 acctatagaa aaattgtctg ttattataat ctctgctctt gtactcacca aaaagacccg
    22021 cttttatctg tgaaacaatt gtatggcctt tccctaatga aagagtgtaa tttagagatc
    22081 ctgaaaatgt gtgttcatca agattgacaa aatctctggt tatttcattt tgatctatag
    22141 ccatcattcc ataatattta tcaccataaa tcaaattttc ttctctgttt atcaatcgtc
    22201 tgtctggttg gttaagacct gcatatgaat agccggcgat ccagtcgata tctgaacttc
    22261 taattttatg gcttcctgca aattgtccgg aataggtggt tctgctcgaa tataaatact
    22321 ccctattttc ctgcatatag agagcactta tattctgcca gccatttctg atagtatatc
    22381 tatttttacc ctgctgattg aaaatatttc taaattcaaa tttgttattc cccttaatac
    22441 aggtaatatt gagcatgcct ccgagttttg cgtctatact gtattgattg tctacatatt
    22501 tgtaaatata ctctggttcg tcagaaacag tattgtatac accaaatcga gcattcagca
    22561 tatctgagta ggtatgtgaa ctgtaggaat agttcaaagc tcctgtaatt ccaagctttg
    22621 tacctttttc ggtaaacctg taacgactaa tcatcaaatt tattctctga tctgcaattg
    22681 gcctgtgttc atgcactctc cagtcatttc taaagcctct tgatgttact tcatctacct
    22741 gagaaggatt attgttatca agtctctgcg gaactacctt cctgatagcc ctttcaccac
    22801 tgtcgaaccc cagaaaatct gtaggtgatg aggtattgta aaaatgatcg ttaaaatgag
    22861 tacttgtatt aaaattcaat ccataactta agagcgtttc attggactca ggcatatttt
    22921 tcgtcattat cttcacaaaa ccacctgaaa aatccgatgg aatctctgct gatggagatt
    22981 taacaatcat aatactctct atctgagaac tcggcatcat atcaaaagaa aaggctcgtg
    23041 tatctgcctc ggtacttgga actgaagcat tgtttatcca aacattattg tatcttccag
    23101 ataatcccct taccattata tatctatcat caattaccga aaccccggga atccttttga
    23161 caacctctgc tgcactcctg tcctgagatt tggaaatttg ctggcttgat attccgctca
    23221 taactatatc ggcacttttt atcgactgaa taacagctat ttcagaactc attcttttgg
    23281 cagatgtcac aacaatctga tttaatatgt tactggcctc ctccattatt atatcaaatt
    23341 tcacagaagg agcatttatt ctaagatctt tgacttttac ggaattatag gaaatagaag
    23401 atatctccaa attataaata cctcggggaa tatttgtaat aacataatta cctgcagtat
    23461 cagtaactcc cccctttttt atgctgtcaa tatacacgga agcccctatc acaggtaatc
    23521 ctgtattt
//
LOCUS       DAOTQL010000010        23345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2233_length_23345_cov_5.235766, whole genome shotgun sequence.
ACCESSION   DAOTQL010000010 DAOTQL010000000
VERSION     DAOTQL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23345
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2233_length_23345_cov_5.235766"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="P5293_01240"
     CDS             <1..1046
                     /locus_tag="P5293_01240"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR48592.1"
                     /translation="KPEAVEWCNEVDSSALAAEWNDITSAFNNFFKSCKGQRKLRVGE
                     PKFKSRKYSQVAITWTSMAKPKILKNGYLFLTRKLGPVKGTFHRWAEGDFKHATIKQT
                     PTGKWFVKICVEKKDEPKNQNGKSVGIDWNCRDEDFIVMSNGTKVKCPRFLQRSSKQL
                     SHQQKIMSKRFVKGLETQSSNYYKQKQKVAKLHEKVSNQRKDWLHKLSRQICNEYETI
                     VVEDTNLQTMSKMNHGKVVGDQGFGMLRQMIAYKGNLVKVNPKNTSKTCHCCGYINPK
                     VKIGVNKWKCPNCGAEHDRDINAALNILHKWNMTGQSIVGRERAESKNACGEPSSSKK
                     QEVPNPSSRNIGS"
     gene            1164..1295
                     /locus_tag="P5293_01245"
     CDS             1164..1295
                     /locus_tag="P5293_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48593.1"
                     /translation="MAGKVDDPEAYCASIKDRAWGSTLWRGKGKTRKEIEKQIKARE"
     gene            1461..1892
                     /locus_tag="P5293_01250"
     CDS             1461..1892
                     /locus_tag="P5293_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48594.1"
                     /translation="MLLKDDDDSTIINGKRICIDFDGVIHKYSRKYHDGSIYDKPTEG
                     VREFLHRLKELGYCIFVHTSRPLAANNPEQAIKLMEKWMKRWKIPYDFITMIKVPANA
                     YIDDRAIRFTGDWKYVMKELDDIENINKDEGEWEYYHKKIV"
     gene            1947..2483
                     /locus_tag="P5293_01255"
     CDS             1947..2483
                     /locus_tag="P5293_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48595.1"
                     /translation="MRKGASKSKGSSFEREISRFLTKWASGQTEELYYWRSPSSGAVA
                     TLNVGNKDISGDIIALRPEGAFLTDRFSIEIKTGYSKASFHQHLKDNKNFDIENFWLQ
                     CVDAADRAGKKPMLIYRKLGFPIIVGIGCDTRIRLKEKMKLPKSVHLIFENKTPSIAF
                     YDMKEFFKIITPNVIKEL"
     gene            2480..2860
                     /locus_tag="P5293_01260"
     CDS             2480..2860
                     /locus_tag="P5293_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48596.1"
                     /translation="MRLKFTSSEFADFSAAFYYDRLTNPDMIPYQVNEDFDDLIEVLK
                     RKGCRQIFLAYYLHMNPQIRVKYRMGKDTFRLGKGKQSLLIEIVPDKVPEEMIQVGFL
                     RKVGRLLKKFLKGEFTEKQPTRKV"
     gene            2870..3181
                     /locus_tag="P5293_01265"
     CDS             2870..3181
                     /locus_tag="P5293_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48597.1"
                     /translation="MPGEFMGQETISPEQTAIQNTKFDGKMEQVPIEGIEGLFADGIN
                     QKDNLPIFNVDKEDFYQNMSHGRKRIRFKSGSAVQQFMQKSRYNRSFYIKHGDYIRKV
                     K"
     gene            3369..3641
                     /locus_tag="P5293_01270"
     CDS             3369..3641
                     /locus_tag="P5293_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48598.1"
                     /translation="MKYRAFLAVQEAAYMGNIGFAEMVRFYKKASESEIKEMEKTISN
                     GNWYAFKSLIKKVLGVDLQEQGQNVYLAYQGDTKILRKNDEEEEEE"
     gene            3638..3889
                     /locus_tag="P5293_01275"
     CDS             3638..3889
                     /locus_tag="P5293_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48599.1"
                     /translation="MSSIREYLTESKGLAKYYTNVLDELISEFDSLIKSMETESTRYL
                     EEKQIKEVKRALKTCERYVELLIGEINKITDFFAASERK"
     gene            3886..4131
                     /locus_tag="P5293_01280"
     CDS             3886..4131
                     /locus_tag="P5293_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48600.1"
                     /translation="MTKIREFLKEDRSIAKHMFKEFGVMLTQIDETIEELGISLNNIP
                     HAYKSPIYDQISELRRLAIRMNKTCKEIIDLLERAKE"
     gene            4220..5092
                     /locus_tag="P5293_01285"
     CDS             4220..5092
                     /locus_tag="P5293_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48601.1"
                     /translation="MKINVFDFNNLAIRIFFLKMIGGHSPQPDYQLWRFLIFDNIVKS
                     LVRNVDTREVILAIDHKNSWRKQFWNGYKRSRKSARDKSGVDWDSFHFELDRYIAELK
                     NHLPFKVIKIRSAEADDIIGVICLKYPDNQYTIISTDEDYAQLCSPNIKVYNPLKKGF
                     LKCNNPEMFIIEKCLLGQKKDDIPNIKTPLDWPMNKRRPSFGSTALKKVMEEGYQKWL
                     KKNKLEKRFRENKILIDFREIPKVIQQRVLEEYHKYLMPPPEQLYEFLAKNKFKTYQE
                     NFHDLERKMINLYR"
     gene            5097..5357
                     /locus_tag="P5293_01290"
     CDS             5097..5357
                     /locus_tag="P5293_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48602.1"
                     /translation="MFDDILKKRSNKKEKKLNLLTKKDKLTAEEIIFLLGVEFEKIYE
                     DHLIKFTSKENYAAKLENIILDALIRGDYPFQSVLGNPKPNP"
     gene            5369..5608
                     /locus_tag="P5293_01295"
     CDS             5369..5608
                     /locus_tag="P5293_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48603.1"
                     /translation="MTLYAVINDEERTRFEKETHLPYGYGSQDVYLTHDYFNALAYFN
                     TIPPHIKTYYVVEKIECGIAEKIFPVVSFTNNEFH"
     gene            5622..8975
                     /locus_tag="P5293_01300"
     CDS             5622..8975
                     /locus_tag="P5293_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRR48604.1"
                     /translation="MLSENAKKIIKDLYSINGEDVDDVFKRVAKEFATNETELNLAFR
                     LQKENVWRPNTPVYFNAGSDHPMFSACWVVGLEDTMNSIYDIANVARKIFQHGAGIGI
                     PIGNLRGKDAHIYEGKPDGPPTGKSSGPISFMKLYDAVGETTKSGGRARRAAILCCMP
                     VWHPDIMEFISCKEIDGRLSNMNISVSITDGFMKALEQNTPYQLINPANGEMTGEVIP
                     LELWNKLTEMAWKSAEPGVLFIDTINKDNPLKKITLIESTNPCVTGDTLIAVADGRNA
                     VSIRQLAEEGKDIPVYCKDNNGITKIRMMRNPRITGYKQKIYSVNLDDGSCIKCTGNH
                     KFLMKDGSIKEAKTLLSNDSIMISSKWKATIEEIIKNSNSKSQEYWLTNNGKKNVFEH
                     RLIYEELSGKKIPKGYVIHHKDHNSLNNNFDNLVPILKEEHDNLHDISGDKNPMRRFP
                     EKNIFNNPEVQRRIREKYHIGAKRTKETKKRIGEKTKERFLDASFRQKHSEAVSRFMK
                     KSGKFDEFIHNRAVKYLEECKKKTDLECYLDENIVKVKRRCEWCNKEFYVPFKNREQS
                     FCSYSCSIHSRYNIFSVNETAENHKIVSVIEDGYEDVYNGTVDEYHNFGIILNKEKIT
                     SYGRQKLEIIYTLQCGEQPLWPFTSCNLSSINIAKFCKDGKFDFEALYKVTYDIMGLM
                     DNIIDKMSFPDERFKVNVLKYRPVGIGIMGLSDAMFELDLKYDSNEGRQFAGKVMKTM
                     TTACVEKSADLAAKKGKFENYDIVKNDIEEIISKHIGGNEKVMAKVRKHGLRNVQFTT
                     CPPTGTTALTADASYGIEPSFGLVFQKTLTETGETINIVNPIFKKRFEKEDWYNEGLM
                     AKITANGGSLKGLHGIPKKVRDIFVCAHDIKYKDRVDMQAELQKYCSSGISSTVNLPS
                     TTTKEEISDLYKYAYKQGLKGITIYRDGCRQSQPITFSKSGTEVQSNFKRPSKLPGQV
                     YTIETGNGKLYITITAHNNRPVEIFMSMGKSGQLFNVFGEALGRVISIALQHGVPLDS
                     IIKTMKGIYSERPTWTRFEETDNRPVQILSIPDAVAKLLERYYLHDGKEEQDDHIDDK
                     LFCTKCGTYSVILVEGCKTCLNCSESACS"
     gene            9038..9379
                     /locus_tag="P5293_01305"
     CDS             9038..9379
                     /locus_tag="P5293_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48605.1"
                     /translation="MKRNLTRKTMREDFQPVLPYHDKKIAEVARKMGITRQAASQTLK
                     RALKKFYFCVQKLDETWTPFQVALIMAKMLNQDHSIKEMRTFIRLLPRDIGLLIWNDA
                     MKRMPGALIEK"
     gene            9376..9543
                     /locus_tag="P5293_01310"
     CDS             9376..9543
                     /locus_tag="P5293_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48606.1"
                     /translation="MKEENIYPSCINCFYCKTKKGAVYCKNGKFKESNVKKILLYTPI
                     KFDCEDFEAMD"
     gene            9546..10586
                     /locus_tag="P5293_01315"
     CDS             9546..10586
                     /locus_tag="P5293_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48607.1"
                     /translation="MISLQTVREFVFQHFPKVTTSKNGTHFHARCIICGDSKISPNKK
                     RFHLDYHNGEPGYHCFNCGAKGSFVKLYSIVMGVSLADAKRELYKYDPDRIRKELTEK
                     PNVNGNGKKEYHIQNMDNILDECIDVYTEVRGSIEPQYQKLLKEFIKDRKIPDDVKIF
                     VAVRGRYKGRAIIPIYKDGHIIYFQGRALTNIEPKYLNPIVEKSGIIMNETKFQRDKY
                     IIVTEGLLDAIMIGPQGTSVLGADIDEEFVKRVNSLTDAGVIIVLDNDKTGYEKLMGK
                     KKNEGLLDKSYSNYLKFFIMPHKYEKIKDINELVKKHNIENVYDFIVENSHNKFETKT
                     RLGLVKKKFGGW"
     gene            10588..11043
                     /locus_tag="P5293_01320"
     CDS             10588..11043
                     /locus_tag="P5293_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48608.1"
                     /translation="MKLTVIGSDYIVINEDNLRDENILKIPRVHLIELAFQDPTKEKI
                     EAVLTLYSKTNRFVISDNIRIYNALLKQTAKKYYVKNTAGVGLISFFRKNNKVLLDTI
                     VLSEQEKSFVLDTLLPDILRNIEVIRVSKSDFEKYKAKLEPWNGNVIVV"
     gene            11211..11864
                     /locus_tag="P5293_01325"
     CDS             11211..11864
                     /locus_tag="P5293_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48609.1"
                     /translation="MPIYHHLTRNELSQTGSIHKNLPIKDYSLMIKIFKEEILKRTNV
                     NKRDITVVNVPYTKNSNFPFYNKYFSKIECPDIKIKKKYSVVYIPDISEDEERLVEIY
                     KLLKKNYDVGVIGDLKTYLEDVNVVTRRVDYFENGYKYIIKYIMEADIIICPASHWTL
                     LCNQQKVPVFSWGENIGRYSKMGIYNLGNEKCFLTPTTSETPAKEIFKTMQYFINSF"
     gene            complement(11851..12297)
                     /locus_tag="P5293_01330"
     CDS             complement(11851..12297)
                     /locus_tag="P5293_01330"
                     /inference="COORDINATES: protein motif:HMM:NF039820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HRR48610.1"
                     /translation="MIECLKEGMIIEGLMHDLSKFSLLEFKAYANHFYGKNRDMYTYI
                     SHNGYSKDDDPIKDIDFDIAWVHHIHHNPHHWQYWVVLENEKQGVILEMPRKYMVEMI
                     CDWIGAAKAQGDFRRSKLKLWYNINKNKIILAQNTRKWIEYRIKSY"
     gene            complement(12290..12607)
                     /locus_tag="P5293_01335"
     CDS             complement(12290..12607)
                     /locus_tag="P5293_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HRR48611.1"
                     /translation="MTKVVHCKKERYDVFIGRPSKWGNPFIIGKDGNRAECIRKYRSW
                     LMSQPSLLEDLQELEGKVLGCYCKPKACHGDVLVELLEKRIDNEIFEIFEIPSKAQIL
                     CND"
     gene            complement(12615..13091)
                     /locus_tag="P5293_01340"
     CDS             complement(12615..13091)
                     /locus_tag="P5293_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48612.1"
                     /translation="MPPLSDKVIKGREWKTKRGKYTYDEVKNIIETEGYTLCDDIYTN
                     SRTKLTVRCPKGHEREMRFDNWLAGHRCATCGNHREISPKWDIARKDIEKRGYKVLAE
                     PEKSPAKAKLLCPKGHIFEISIYNWLRGSRCPKCPRTARYFNDREFLNLSKCYRKK"
     gene            complement(13072..13842)
                     /locus_tag="P5293_01345"
     CDS             complement(13072..13842)
                     /locus_tag="P5293_01345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRR48613.1"
                     /translation="MNLSRSKRLSGIDMRFIIVSCGYNAEKYVEANMKSIQSQLYKNY
                     VHLIVDDASTDGTWEKIKNFADDKTVAYRNIENQKWIKNALQYLPPNINSEEDVIVLV
                     DLDDSLAHNTVLNKVEKAYRKHNCWMTYSLFRYSNGMTSNWIPRYTKEVYEKKLYRRH
                     IWLFGHMRTFKAFLWNNLKDEDLRDENGNYCIYAYDQVILMPMLEMCPPNKIYFIDDV
                     MYNYNDDNPLQVEKINKSLQSKTSRYIRSRPSYATLIR"
     gene            complement(13805..14911)
                     /locus_tag="P5293_01350"
     CDS             complement(13805..14911)
                     /locus_tag="P5293_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48614.1"
                     /translation="MNLVVILPTRQNKNLTEKIQSKLGQNDRLIAVDIPKFPARKLDE
                     IIWRDISGQDYNILIISPLTVDIADDFIEVFKFFVPETENILVRSPLEEFDDFYFTND
                     AGLLRSSDCILFRKSLYKRQLGDIMPHADYLLILKELVKNVGRGGYYTPLRDQKKLQL
                     LFEEEERSRKTEEERKKELLLQKLNAIKERRKLREEERNKRSISLQKLKEDISRLEEE
                     LSRQEEELSRQEAEFTNSENIIASKIIEKENELKRIKLRIADKEILLKRIKEAECSRE
                     YIRKISDEIDRYKKMEQEMLEAQNSVIVNIQDENSMRKISLKRRIEERQMPRKKSRRG
                     PQWIQENQEIRRIILSMEPEKSNEPIKIKKIEWD"
     gene            complement(14908..15180)
                     /locus_tag="P5293_01355"
     CDS             complement(14908..15180)
                     /locus_tag="P5293_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48615.1"
                     /translation="MCPIYDFECKKCNKLIEVYESVTKSEEVHKCDCGGIMIKVMPTK
                     MTFELLYDPKKDLVSWGNEGYARTQRYREYDKQAKKNIFPVSGGKK"
     gene            complement(15240..15710)
                     /locus_tag="P5293_01360"
     CDS             complement(15240..15710)
                     /locus_tag="P5293_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48616.1"
                     /translation="MKWYQFRIRVVFLGAILVYFISNPLISLIDYDKPMELNFCPPCI
                     IVYKSNLVDDVRAASKGMVIYIRPEDKGNIAILQHELTHCKQYWLSAGMNVFLYHYFD
                     KFRLMYELEAFKEQIKYENEPEKARIEAALLLSNHYSLSKLINFEMAYLLLEDK"
     gene            complement(15720..16400)
                     /locus_tag="P5293_01365"
     CDS             complement(15720..16400)
                     /locus_tag="P5293_01365"
                     /inference="COORDINATES: protein motif:HMM:NF036314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48617.1"
                     /translation="MRLYRYIKEVTAGEKYSKEMAAKYRGVPIEQIPTEDLWVAMMRG
                     NTKLAKNIKTFNLPAGGSCPNMKECYKDCYAKKAEKMRPVVRISRERNFRLAKENTQL
                     LKKRILQHLEKGDVVRIHESGDFFSQEYLDMWYEIAKERPDVMFYTYSKTEHLWDWSK
                     IKRLPNFNLVSSIVGGKINFGPEEEIKIRAQELGVPICPCRKGNKVKCGIDCKICWTE
                     PYVLFIKH"
     gene            complement(16426..17319)
                     /locus_tag="P5293_01370"
     CDS             complement(16426..17319)
                     /locus_tag="P5293_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48618.1"
                     /translation="MRLKTYLNENSNLKIHPFEEAGLGKYPYKFLYARREVYVAYPGA
                     PVQPGGSCDYCSTGIYFQFWLESADKKKFKVGSECIMKASRKSATEPVSKEDYLLRKA
                     VMDFKRNQVKDAQKERIRIALDFLDKNQDFREVLNAIPRPGWKGEQSMLDYINWMREH
                     SGLAGKMKITKYIENLVKKNWKSMPKKAIGSKQETGKAPYTQNKKAYKGIPGPYDDKN
                     IPQQLIMGMYERQKRNAIEAVLQDEKFRSFLKTIPHPKGFKNKSYLDFSLWLISNQYL
                     APKHVTELEDYWEELYKIKIR"
     gene            complement(17351..17429)
                     /locus_tag="P5293_01375"
     tRNA            complement(17351..17429)
                     /locus_tag="P5293_01375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17394..17396),aa:Arg,seq:acg)
     gene            complement(17776..17852)
                     /locus_tag="P5293_01380"
     tRNA            complement(17776..17852)
                     /locus_tag="P5293_01380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17816..17818),aa:Arg,seq:tct)
     gene            complement(17911..18399)
                     /locus_tag="P5293_01385"
     CDS             complement(17911..18399)
                     /locus_tag="P5293_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR48619.1"
                     /translation="MRNFLIIVVCLTLVACYTPIKTNVPVGDCPSCKSEPVPVVKATP
                     LPPPPPAPPVVVKKKIDVLSFYFDFDKATLKGQQDAVDRAIEYLKDDPAKTVELQGNC
                     DNRGSDKYNMKLGQKRANTVKNILIKKGIDSKRLTTKSFGKSKATGNHAKDRRVDIVV
                     LK"
     gene            complement(18427..18729)
                     /locus_tag="P5293_01390"
     CDS             complement(18427..18729)
                     /locus_tag="P5293_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48620.1"
                     /translation="MAVIFTVIVAKENENGLLTVPLGWFTTFPDASSGMSNFVKHIYD
                     DDLEMWTYFIIEMVPEGINTPVKESRWFKWFPEEASYVPIKKPEKYKDIINFSFMK"
     gene            complement(18897..18969)
                     /locus_tag="P5293_01395"
     tRNA            complement(18897..18969)
                     /locus_tag="P5293_01395"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18937..18939),aa:Met,seq:cat)
     gene            complement(18980..19246)
                     /locus_tag="P5293_01400"
     CDS             complement(18980..19246)
                     /locus_tag="P5293_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48621.1"
                     /translation="MLSKLSRSHGACSVECHNIIKIWRKTNVGNTNILADAKRENTCV
                     GHKNQSSYRSDYVITHQYDMDFVKDLWNSCGYSWYNVFDILVVY"
     gene            complement(19363..19872)
                     /locus_tag="P5293_01405"
     CDS             complement(19363..19872)
                     /locus_tag="P5293_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48622.1"
                     /translation="MKKILLVILLILCFSIPSHASDVQWGFSIGIGQPGFYGRIDIGS
                     GYPAPMCVYPQPMFAVYDPYYNNMPILYLYVPSIHITNWGAYCHHYNACNRRVHFVQD
                     RWYHNIYVPAYRHRHGDRPPKHYYDHGQYRGNPMTRQDPHFDSFGRSQPRPPVFNRGG
                     GFEKGRGHK"
     gene            complement(19956..20300)
                     /locus_tag="P5293_01410"
     CDS             complement(19956..20300)
                     /locus_tag="P5293_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48623.1"
                     /translation="MKKILLAVIMVLCFAVSAFAFGPQEMSVVSMSQCQSSIWGHQGQ
                     AQLLSTTGGAIVAGGNLQAQGGFARGSYFNAERHRCSSSVSYGSFTSASGQFQAQGAV
                     IIPVQNHYYRPN"
     gene            complement(20367..21479)
                     /locus_tag="P5293_01415"
     CDS             complement(20367..21479)
                     /locus_tag="P5293_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48624.1"
                     /translation="MLKRVLLSFIFVLALSTMAFANSGDTWATLGGQANSGANGAQNI
                     GSGIANTGNNNQFGGTSATVNGGITNTATGGAATATATNTNTNTATGGAGGSATVRDS
                     GNSFNLNNNQATVRDSGNSYNRNSNRNTNVNNIDMNQRQAQGQTQGQMQGQELSGNQS
                     TANVSITQNGAPIPRNFAIPGTVYYPSMPSEFLNDTQFANAQPVKFILQFGCVYNRAH
                     LENMKGDGWVKTLQLVNLYGNKAEPADTDTITFLTELPKDLKLVKIGSIVSKAKTGGT
                     TTEQVLGDMGLEALSIKGAKYVLISGEGIQKYTKTSGWGVALGYTQAGISDDGQRAGT
                     GTAGIGYSSGSAGREANPFIHGTVLTDEATFNTLMK"
     gene            complement(21576..21854)
                     /locus_tag="P5293_01420"
     CDS             complement(21576..21854)
                     /locus_tag="P5293_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48625.1"
                     /translation="MKKFVLLVVIVFLLPNLFSITVSAQMSLPEKEFIVKEKVKEQDK
                     IIKEDARKKKIIYNRAMEEEKQSTINNSVVQSYVPAESQSIKIKNNSR"
     gene            complement(21865..22362)
                     /locus_tag="P5293_01425"
     CDS             complement(21865..22362)
                     /locus_tag="P5293_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48626.1"
                     /translation="MKKVVFLCVILMVVMGCATVKVVDERGMPAPNYETVLSQPSYGF
                     QTVQSIVRTVEVSPESYEPEYLDLFKKYRIPKDKTKAITITFRLVNPKGAKYILAKTI
                     EYKTNNEFEFSKRISILHRGSDKDKTWVISCPLDYGTYRVSIDVTDDNGNLIVTHGLF
                     TYIIK"
     gene            complement(22414..22716)
                     /locus_tag="P5293_01430"
     CDS             complement(22414..22716)
                     /locus_tag="P5293_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48627.1"
                     /translation="MKRVKKSKTYDTPKSSGDKGSQIGRNIRNLRTMVRSYTTGEVRF
                     DRRKKAFNEILDYEKKVRKALLKLSKKDRILYESEIDTFLHQDYKEHIKWISKNEK"
     gene            complement(22848..23237)
                     /locus_tag="P5293_01435"
     CDS             complement(22848..23237)
                     /locus_tag="P5293_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48628.1"
                     /translation="MNKFRYIAWRDEKDEFKVSILVEERQPDGLDLGLPLKQVVRHSP
                     TGMEWGYGGSGPSDTALSILTDYYVRSGLRSSRKEAIEAASKNGLYMDFKWEFIAPAE
                     KEGFKITDVQIRDWLFKQAEKMDSELG"
BASE COUNT         7651 a         4322 c         4251 g         7121 t
ORIGIN      
        1 gtaaacctga agctgtggaa tggtgtaatg aagtagacag ttcagcatta gcagcagaat
       61 ggaatgatat aacttcagct ttcaataact tcttcaaatc ttgtaaagga caaagaaaac
      121 ttagagttgg agaacctaag ttcaaaagta gaaaatactc acaagttgct ataacttgga
      181 catctatggc aaaacctaaa atactaaaga atggatattt gtttcttaca agaaaacttg
      241 gtccagttaa aggaacattt catagatggg cagaagggga tttcaaacac gctacaatca
      301 agcaaactcc aacaggaaag tggtttgtaa agatttgtgt tgaaaagaaa gatgaaccta
      361 aaaatcaaaa tgggaaatca gttggtatag attggaactg tagagatgag gatttcatcg
      421 taatgtcgaa tggcacgaaa gtcaagtgcc ctagattctt acaaaggtct tctaaacagt
      481 tgagtcacca acagaaaata atgtcgaaaa gattcgtaaa aggtcttgag acacaaagtt
      541 ccaactacta caaacagaaa caaaaagttg caaagttgca cgagaaagtt tccaatcaaa
      601 gaaaagattg gttacataaa ctttcaagac aaatatgtaa cgagtatgaa actatagtag
      661 ttgaagacac caacttacaa acaatgtcta aaatgaatca cggaaaagta gtaggtgacc
      721 aaggttttgg tatgcttaga cagatgattg cttacaaagg taaccttgta aaagtcaatc
      781 caaagaacac ttcaaaaact tgccattgtt gtggatatat aaatccaaaa gtaaagattg
      841 gtgtaaataa atggaaatgt ccaaactgtg gtgcagagca cgacagagac ataaatgcag
      901 ctctaaatat tctacataag tggaatatga ctggtcagag catagtaggc agggaacgag
      961 ccgaaagtaa aaatgcctgt ggagaaccaa gcagttctaa gaagcaggaa gttcctaacc
     1021 catcaagtcg aaatattggc tcgtaaggct aggaagatat tcggtcttta gccgaatatc
     1081 cgttcactag agaatatctt attgaaaaag aagttggagt tcatcctaaa gaagtaggat
     1141 ttttcgatgc ctgtgtcaaa agaatggctg ggaaagtcga tgatcctgaa gcttactgtg
     1201 catccataaa ggacagagca tgggggtcaa ctctttggag aggcaaaggt aagacaagaa
     1261 aagaaattga gaaacaaatc aaagcaagag aataaaaaat aaaaccagat gtcagatacg
     1321 ataatatcat ttatcatctg ggccgtgacg gttgttatcc tcatcttgga tattaaaatt
     1381 acaaaactaa ttaccagagg aaaacctatc ccgaaagttg ctgagaaaat tttgggtatt
     1441 aaaaaggaga aagagtgaat atgttattga aagacgatga tgatagtacg atcatcaatg
     1501 gtaaaagaat ttgtattgat tttgatggtg ttatacacaa atactcaaga aaatatcacg
     1561 acggctcaat atatgataaa ccaacagagg gggttagaga atttctgcat agactcaaag
     1621 aattgggtta ttgtatattc gtgcatactt cacgaccact tgctgcaaac aatccagagc
     1681 aagcaatcaa attgatggag aagtggatga agaggtggaa aattccttac gactttatca
     1741 cgatgataaa agttcctgca aatgcttaca tagatgatag ggctatcagg ttcaccggag
     1801 attggaaata tgtgatgaaa gaactggatg acatagaaaa cataaataaa gatgaaggag
     1861 aatgggagta ttaccataaa aagattgtat gaggataaag ggcgagaaaa atccaatata
     1921 tagaacaaaa tattataata gaaattatgc gaaaaggagc aagtaaatca aaaggatcat
     1981 cttttgaaag agaaataagt aggtttttga caaaatgggc ttccggacaa acagaagagt
     2041 tatattattg gagaagccca agtagtggcg ctgtagcaac tttaaatgtt ggaaacaaag
     2101 atatatccgg tgatataata gctctcaggc cagagggggc atttcttact gatagattta
     2161 gtattgaaat taaaaccgga tattcaaaag caagctttca tcaacactta aaggacaaca
     2221 aaaattttga tatagaaaat ttctggctac aatgtgttga tgccgcagat agggcgggca
     2281 agaaaccaat gttgatatac aggaaacttg gctttccaat tattgtgggg ataggatgtg
     2341 ataccagaat cagattgaaa gagaagatga aactgccaaa gtcagttcac cttatttttg
     2401 aaaataagac accttcaatt gcattttatg atatgaagga gttcttcaaa ataataacac
     2461 cgaatgtaat aaaggaatta tgagattaaa atttaccagt tctgaatttg ctgacttttc
     2521 tgcggccttc tattatgata gattgacaaa tcctgatatg ataccctatc aggtaaatga
     2581 agattttgat gatttgatag aggttttgaa aaggaaagga tgtagacaga tatttctcgc
     2641 ttattatctc catatgaacc ctcagataag ggtaaagtac agaatgggaa aagatacatt
     2701 cagattaggg aagggaaaac aatctcttct aatagaaatt gttccggaca aggtgccgga
     2761 agaaatgata caagtaggtt ttttgcgaaa agtgggaagg ctactgaaaa aattcctcaa
     2821 aggagaattt acagagaaac aaccaacaag gaaagtatag gaggacatta tgcctggaga
     2881 atttatgggt caggaaacaa tatctcccga acaaacagca atacagaata ctaagtttga
     2941 tgggaagatg gaacaagttc caattgaagg tattgaagga ttatttgctg acggtattaa
     3001 tcagaaagat aatctaccga tttttaatgt tgacaaggaa gatttctatc agaatatgtc
     3061 acacggtaga aaaaggatta gatttaaatc tggttctgct gtacagcagt ttatgcaaaa
     3121 atcaagatat aacaggtcat tttatattaa gcacggagat tatattcgaa aagtaaaatg
     3181 aatataaacc aaaaggggtt agctaaaatt ttaaagggtc ttatggcatc aatattatgt
     3241 ttagctaagg tgtgtgatac tcttattcat agtaggacaa agaccagagg ttccaattca
     3301 acacatgatg tagaaaatca catcaagaga atatttttag aatatggaat gatagataaa
     3361 gaggaaaaat gaagtataga gcgttcctcg ctgttcaaga agccgcttat atgggaaaca
     3421 tcggtttcgc ggagatggtg agattttaca aaaaagcctc ggaaagtgaa attaaagaaa
     3481 tggaaaagac catttctaat ggtaattggt acgcctttaa atccctgata aaaaaagtct
     3541 tgggtgtaga tttacaggaa cagggccaga atgtttatct cgcatatcaa ggcgacacaa
     3601 aaatactaag aaagaatgat gaagaggagg aagaagaatg agtagtataa gagaatattt
     3661 aacagaaagt aaaggcttag cgaaatatta tacaaatgtt cttgatgaac taatatctga
     3721 attcgattct cttataaaaa gcatggaaac ggaatcaacc agatatttag aagaaaaaca
     3781 gataaaggaa gtaaagagag ccttgaaaac ttgtgaaagg tatgttgaat tgttgatagg
     3841 agagataaat aaaatcacag acttttttgc agcaagtgaa agaaaatgac aaaaattaga
     3901 gaatttctca aagaagatag aagtattgca aaacatatgt tcaaggaatt tggtgttatg
     3961 ctaacccaaa ttgatgagac catagaagag ctcggcatat ctctaaataa tatacctcac
     4021 gcatataaat ctcccatata cgaccagata tcggaattga gaaggctcgc aataagaatg
     4081 aacaagactt gtaaagaaat aatagattta ctggaaaggg caaaagaatg acgaagagcg
     4141 gtaaataaag caatagctga tttgcctctt gaaaagaaat agaataagaa atagataaat
     4201 aattataaag gaatttgtga tgaagataaa tgtttttgat tttaacaatc ttgccatccg
     4261 tatcttcttt ctgaagatga ttggtggaca ctctccacaa ccagattacc aattatggag
     4321 atttctgata tttgataata ttgtgaaatc tcttgtacga aatgtagata caagagaggt
     4381 tattctcgcc atagaccata aaaattcgtg gagaaagcag ttttggaatg gatacaaacg
     4441 ctcgagaaaa tccgcgagag ataaaagtgg tgttgattgg gatagctttc atttcgagct
     4501 tgacagatat attgcggaac tgaaaaacca tcttccattc aaagttatta aaatcaggtc
     4561 tgccgaggct gatgatatca tcggtgttat ctgtctaaaa taccccgata accagtatac
     4621 cattatatca actgatgaag attatgcaca actatgttct ccgaatataa aagtttacaa
     4681 tcctctaaag aagggcttct tgaaatgtaa caatccagaa atgtttatta tcgaaaaatg
     4741 tctcctcggc cagaaaaaag atgatattcc caatataaaa acacctttgg attggccaat
     4801 gaataaacgc agaccgtcct ttggcagtac cgccttgaag aaagtgatgg aagaaggata
     4861 tcaaaaatgg ctaaagaaaa acaaattaga aaaaagattt agagaaaata aaattctaat
     4921 tgatttcaga gagataccaa aagtaattca gcaacgagtt ttggaagaat atcataaata
     4981 tttaatgcca ccaccagaac agttatatga atttctggcc aaaaataagt tcaaaacata
     5041 tcaggaaaat tttcatgatc ttgagagaaa aatgataaat ttatataggt aataaaatgt
     5101 ttgatgatat tctaaaaaaa agaagcaata aaaaagaaaa aaaattaaat cttctaacaa
     5161 agaaagataa attaactgca gaagaaatta ttttcctttt gggagttgaa tttgaaaaga
     5221 tatatgaaga tcaccttata aaatttacct caaaggaaaa ttatgccgca aaacttgaaa
     5281 acataattct tgatgcactt ataagaggag actacccatt tcaatcagtt cttggaaacc
     5341 ctaaacctaa tccatagagg aggttacaat gaccctatat gccgttataa atgacgagga
     5401 gcggacaaga tttgagaagg agacacatct tccatatggt tatggaagtc aggatgttta
     5461 tttaacacac gactatttta acgcattagc atattttaat actattccac cccacataaa
     5521 gacatattat gtagtggaaa aaatagaatg tggtattgca gaaaagatat ttccggttgt
     5581 atcctttacc aataatgaat ttcattaagg gagttatgta gatgttatcg gagaatgcaa
     5641 agaaaataat aaaagacctt tatagtatca atggggaaga tgttgatgat gtattcaaaa
     5701 gagtcgccaa ggaatttgcg accaatgaaa cagaattaaa tcttgccttc agattacaaa
     5761 aggagaatgt ttggcgtccg aatacgcctg tatatttcaa tgctgggtct gatcacccaa
     5821 tgttttccgc ctgttgggtt gtaggacttg aagatacgat gaacagtatc tatgatattg
     5881 caaatgttgc aagaaagatt tttcagcatg gagcaggaat tggtatcccc attggaaatt
     5941 taagaggaaa ggacgcgcat atttatgagg ggaaacccga tgggccgccg acaggaaaat
     6001 cctctggtcc catcagtttt atgaagttat atgatgccgt aggtgagact acgaaaagtg
     6061 gtggaagagc cagaagagcg gccatattat gctgtatgcc tgtatggcat ccagacatta
     6121 tggagtttat atcctgtaag gaaattgacg gccggctcag taatatgaac atctccgttt
     6181 ccattacaga cggttttatg aaggctctgg aacagaatac accctatcaa ctcatcaatc
     6241 cagcaaatgg tgaaatgacg ggagaagtaa tcccattaga attgtggaac aagttgacgg
     6301 agatggcttg gaagagcgca gagcccggtg tactgttcat tgataccata aacaaagata
     6361 atcccctcaa gaaaatcacc ctgatagaga gtaccaatcc atgcgttaca ggagacacat
     6421 taattgctgt tgcagatgga cgaaatgcgg tttcaattag gcagcttgca gaagaaggga
     6481 aggatattcc tgtttattgt aaggataata atggaataac aaaaataaga atgatgcgaa
     6541 atccaagaat aacaggatat aaacaaaaaa tatattctgt aaatcttgat gatggtagtt
     6601 gtataaaatg tactggaaat cataaatttc ttatgaagga cggttctatc aaagaagcaa
     6661 aaactcttct ttcaaatgat agtataatga tttcctcaaa atggaaagct accattgagg
     6721 agattataaa aaattcaaat tcaaaatcac aagaatattg gctaactaac aatggtaaaa
     6781 aaaatgtatt tgaacataga ttgatatatg aagaattgtc tggtaagaaa ataccaaaag
     6841 gttatgttat tcatcataaa gaccataata gtctgaataa taattttgat aatcttgtgc
     6901 ctattctgaa agaagagcac gataacttgc acgatatctc tggtgataag aatccaatga
     6961 gaagatttcc agaaaagaac atcttcaata atccagaagt tcaaaggcga ataagagaaa
     7021 aatatcatat tggtgctaag agaacaaaag agactaagaa aagaattgga gaaaagacaa
     7081 aagaaagatt tttggatgcc tcttttcgtc agaaacatag tgaagccgtt tcaaggttta
     7141 tgaaaaaatc gggcaaattt gatgaattta tccataatag agctgtaaaa tatcttgaag
     7201 aatgtaaaaa gaaaactgat ctcgaatgtt atcttgatga aaatatcgta aaggtaaaga
     7261 gaagatgtga gtggtgtaat aaagaatttt atgttccctt caaaaatagg gaacagtcat
     7321 tctgttcata ttcttgttct attcattcaa gatataatat attttctgtc aacgaaaccg
     7381 cagaaaatca caaaattgtt tctgttatag aagatggata tgaagatgtt tataacggaa
     7441 cagttgatga ataccataac ttcggcataa ttctcaataa agaaaaaata acatcttatg
     7501 gccgtcaaaa gcttgaaata atctatacct tgcaatgtgg tgaacagccc ctctggccat
     7561 tcacatcctg taatttgagt tccatcaata tagcaaagtt ttgtaaagat ggtaagtttg
     7621 attttgaagc gttatacaaa gtcacttacg atattatggg attgatggac aacatcattg
     7681 ataaaatgtc attccccgat gaaaggttca aggtgaatgt attgaaatac aggccggtgg
     7741 gaataggtat tatgggtcta tcagatgcaa tgttcgaact cgatttgaaa tatgacagta
     7801 atgaaggtag gcagtttgcc ggtaaagtaa tgaaaactat gactactgcc tgtgtcgaga
     7861 agagtgctga tcttgcggca aaaaaaggaa aatttgaaaa ctatgatatt gtaaagaatg
     7921 acattgaaga aattatatca aaacatatag gaggaaatga aaaggtaatg gccaaggtca
     7981 ggaagcatgg actaagaaat gtccagttta caacttgccc accaacagga acaaccgccc
     8041 ttaccgcaga cgcctcctat ggaattgaac catccttcgg acttgtcttt caaaagactc
     8101 tcacagaaac aggagaaaca atcaatattg taaatccgat atttaaaaag agatttgaaa
     8161 aagaagattg gtacaatgag ggtctaatgg ccaaaataac agcgaacgga ggctcattaa
     8221 aaggtctaca cggtattcca aagaaagtcc gtgatatatt tgtatgtgca cacgatataa
     8281 aatataaaga ccgtgttgat atgcaagctg aattacaaaa atattgttca tcgggaatat
     8341 caagtactgt gaaccttcca agcactacaa ccaaagaaga aatttcagat ctttataaat
     8401 acgcctataa acaaggacta aagggaatta ctatctatag agatggttgt aggcaatctc
     8461 aaccaattac ttttagtaaa tctggcacag aagttcaatc caatttcaaa aggccatcaa
     8521 aattgcctgg ccaagtttat actattgaaa ccggcaacgg gaaactttac ataactatta
     8581 ctgctcataa caatagacca gtagagattt ttatgtcaat gggaaaatcc ggtcaactct
     8641 tcaatgtatt tggtgaggcg cttggaaggg taatatcaat cgcattacaa cacggcgttc
     8701 cgcttgattc catcatcaaa actatgaagg gaatttattc tgaaagacca acttggacaa
     8761 gatttgaaga aacggacaat aggccagttc agatattgag cattccagat gcagttgcga
     8821 aattacttga aagatattat ctgcacgatg gtaaagaaga acaggacgat catatagacg
     8881 ataaactctt ctgtactaaa tgtggaacat attctgttat cttggtagaa ggatgtaaaa
     8941 cctgtttaaa ttgtagtgaa tctgcctgtt cataattgtt tacatttatt ataaatagga
     9001 tataatatag atataataac taatttgtga ggaaattatg aaacgaaatt taacccgtaa
     9061 aactatgcga gaagattttc agccagtttt accttatcat gataagaaga ttgcagaagt
     9121 tgcacgaaaa atgggaataa cccgccaggc ggcatctcaa actctcaaaa gagcactaaa
     9181 aaaattctat ttctgtgttc aaaaattgga tgaaacttgg acaccatttc aggtagctct
     9241 gataatggca aaaatgctaa accaagacca ctcgataaaa gagatgagaa ccttcattcg
     9301 tctattacca agggatatag gacttcttat ttggaatgat gcgatgaaga ggatgccagg
     9361 cgcactaatt gagaaatgaa agaagaaaac atctacccgt cctgcataaa ttgtttttat
     9421 tgtaaaacca agaaaggtgc cgtatattgc aaaaacggca agttcaaaga gagtaatgtg
     9481 aaaaaaatct tactttatac accaattaaa tttgactgcg aagattttga agcgatggat
     9541 taaatatgat atcactacaa actgtaagag aatttgtatt tcaacacttt ccaaaagtaa
     9601 ccacatctaa aaacggaacc cactttcatg cacgctgtat tatttgtgga gattccaaaa
     9661 taagcccgaa taaaaagaga tttcatctcg actatcataa cggtgagcct gggtatcact
     9721 gttttaattg tggtgcaaaa ggctcctttg tcaagttgta ttctatcgtt atgggagttt
     9781 ctttagcaga tgccaagcgg gagctttaca aatatgaccc cgacaggata agaaaagaat
     9841 taacagaaaa acccaatgtc aacggtaacg gtaaaaaaga ataccacatt caaaatatgg
     9901 acaatatact tgatgaatgt atagatgttt atacggaagt caggggatcg atagaaccac
     9961 aatatcagaa acttctcaag gaatttataa aagaccggaa gatacctgat gatgttaaaa
    10021 tatttgtggc tgtaagggga agatataaag gtagagcaat aataccgata tacaaggatg
    10081 gacatattat ctactttcag ggccgggccc taactaatat agaaccaaaa tatctcaacc
    10141 caatagtaga aaaaagtggt atcattatga atgagactaa attccaaagg gataaatata
    10201 ttatagtgac agaaggacta ttggatgcga taatgatagg accacaggga acatctgttc
    10261 ttggtgctga tattgatgag gaatttgtaa agagagtaaa tagtttaact gatgcgggtg
    10321 taataattgt tctcgacaat gataaaacag gatatgaaaa acttatgggc aagaagaaaa
    10381 atgaggggct tcttgataaa agttattcaa attatttaaa attctttata atgccccata
    10441 aatatgaaaa gataaaggat ataaacgagc ttgtcaaaaa gcataatata gaaaatgttt
    10501 atgactttat cgtggaaaac agccataata agtttgaaac aaaaacgagg ctcggtttgg
    10561 tgaagaagaa attcggagga tggtaatatg aaattaactg taattggatc tgattatatc
    10621 gtgatcaacg aggataatct gcgggatgaa aatatattaa aaattcctcg tgttcatctg
    10681 attgaactgg catttcaaga tccgacaaaa gaaaaaatag aagcagtact gacattatat
    10741 tcaaagacaa acagatttgt catttctgac aatatcagga tatacaatgc cctactcaaa
    10801 cagacagcaa aaaaatacta tgtaaagaac acagcggggg tcggtcttat ttcttttttc
    10861 aggaaaaata ataaagttct tctcgatact attgtccttt cggaacagga aaagtctttc
    10921 gttcttgata cattacttcc agatatatta cgaaacattg aagttatcag agtatcaaaa
    10981 agtgattttg agaaatataa ggcaaagctt gagccgtgga atggtaatgt aattgtagtc
    11041 taatggtggt tttggcgtgt ggcccattca taggagattg ggaacaggaa attctcaact
    11101 ttcgaccatt catccaatgg gtttataaat catataaccc aaaggatata tacctgtcaa
    11161 cacatttcaa tagatcattt atctatcaat ggatagatga caaaaatttt ttgccaatat
    11221 atcatcattt aacaagaaat gaattatcac aaaccggctc catccataaa aatttaccaa
    11281 taaaagatta ctctctaatg atcaagatat tcaaagaaga aatcctgaaa agaacaaatg
    11341 tcaataaacg agatatcaca gtagtcaatg taccatacac aaaaaattcg aacttcccat
    11401 tttacaacaa gtacttttca aaaatagaat gtccagatat taaaattaag aaaaaatact
    11461 ctgtggtcta tattccagat ataagtgagg atgaagagag attggtagag atatacaagc
    11521 tactaaagaa gaattacgat gttggtgtta ttggagattt gaaaacttat ctggaagatg
    11581 taaatgtagt cacgcgaaga gttgattatt ttgaaaacgg gtataagtac atcattaaat
    11641 acattatgga ggcagatata ataatctgcc cagcaagtca ttggacatta ctctgcaatc
    11701 aacaaaaagt tcctgtattt tcttggggag aaaacatcgg gagatattca aaaatgggaa
    11761 tttacaatct cggaaatgaa aaatgttttc ttacacccac cacaagtgaa acccccgcaa
    11821 aagaaatttt caaaacaatg caatatttta ttaatagctt ttaatcctgt actcaatcca
    11881 ctttcttgta ttttgtgcaa gaataatttt attcttgttg atattatacc agagttttaa
    11941 tttacttctg cggaagtctc cctgtgcctt tgcggcacca atccaatcac agatcatttc
    12001 taccatatac tttcttggca tttccaatat aacaccctgt ttttcatttt ccagaacaac
    12061 ccaatattgc caatgatgtg gattatgatg aatatgatga acccatgcaa tatcaaaatc
    12121 aatatctttt atcgggtcat catcctttga gtatccattg tgggaaatat aggtatacat
    12181 atctctattc tttccgtaga aatgatttgc ataagctttg aactccaaca acgaaaattt
    12241 ggaaaggtcg tgcattaacc cctctattat cattccttct tttaagcact caatcattac
    12301 atagaatttg tgctttagaa ggtatctcaa atatctcaaa tatttcatta tcaatcctct
    12361 tttctaaaag ttctaccaga acatcaccgt gacaggcttt tggtttgcaa taacacccca
    12421 agaccttacc ctccagctct tgtaaatcct ccaataaaga aggctgagac ataagccaac
    12481 ttctatattt tcttatgcac tcagcccgat tgccgtcttt accaattatg aatggattgc
    12541 cccattttga gggccgccct ataaacacat catatctttc ttttttacaa tgaacaactt
    12601 ttgtcatata ctatttattt ttttctgtag cattttgaca gattcaaaaa ctcccggtcg
    12661 ttgaaatacc ttgcggtcct cggacacttt gggcatctac tgccacggag ccagttatag
    12721 atagaaatct cgaaaatatg tcctttagga cacaatagct ttgcttttgc ggggcttttt
    12781 tccggttccg ctagtacctt gtaccctctt ttttcaatgt ccttacgtgc aatatcccat
    12841 tttggagaga tttccctgtg atttccacaa gttgcacatc tatgtccggc gagccaatta
    12901 tcaaatctca tctctctttc atgacctttt gggcatctta ccgtcaattt tgtcctacta
    12961 ttggtataaa tatcatcaca aagggtataa ccctctgttt ctattatgtt tttcacctca
    13021 tcataggtgt atttccctct ttttgttttc cattctcgtc cttttattac cttatctgat
    13081 aagggtggca taagatggtc tgctcctgat ataccgtgat gttttactct gtaaagattt
    13141 gtttatcttc tcaacctgaa gtggattgtc atcattataa ttatacatta catcgtcaat
    13201 aaagtaaatc ttgtttggtg ggcacatctc caacatcggc atcaaaatca cttggtcata
    13261 ggcatagata caataatttc cattttcatc tctcaagtcc tcatctttca gattgttcca
    13321 gagaaacgcc ttgaatgttc tcatatgacc aaacaaccag atatgcctcc gatatagttt
    13381 tttctcatat acttctttcg tatatctcgg tatccaattc gatgtcatac cattggagta
    13441 tctgaacagg ctatatgtca tccagcaatt atgttttcta tatgcttttt caactttatt
    13501 gagaactgta ttatgggcga gactgtcatc gagatcgaca aggacaatta catcttcttc
    13561 tgaatttata tttggtggta gatattgaag ggcgtttttg atccactttt gattttctat
    13621 atttctatag gcgacagttt tatcatcagc aaagttcttt attttttccc aagttccatc
    13681 cgtcgaggca tcgtccacaa taagatgaac ataattttta tacagctggc tctgtatgct
    13741 cttcatatta gcctcaacat atttttcggc attgtaccca cagcttacta taataaatct
    13801 catatcaatc ccactcaatc tttttgatct tgataggttc attcgatttt tccggttcca
    13861 ttgagaggat aattcttctt atttcctgat tttcctgtat ccattgaggg cctcttctcg
    13921 acttttttct gggcatctgt ctttcctcta tccttctctt cagagagatt tttctcatac
    13981 tgttctcatc ctgtatattg acaattactg aattttgtgc ttccaacatt tcctgttcca
    14041 tctttttata tcggtctatc tcatcactaa tcttccggat atattctcta ctacactccg
    14101 cctcctttat tcttttcaaa agaatttcct tatcggctat tcggagcttg atccttttca
    14161 gttcgttctc cttctctata atttttgatg ctattatatt ttcagaattg gtaaattccg
    14221 cttcctgtct tgataattcc tcttcctgtc ttgataattc ttcctcaagc cttgatatat
    14281 cttcctttaa tttttgtaaa ctaatactcc ttttatttct ttcttcttct cttaatttcc
    14341 ttctctcctt tattgcattg agcttttgta aaaggagctc cttctttctt tcctcttcag
    14401 tttttctgct tctctcttcc tcttcaaata agagctgtaa tttcttttgg tctctcaaag
    14461 gtgtataata accgcctcta cccacatttt ttacaagttc tttcaatatc aaaagataat
    14521 ccgcgtgtgg cattatatct cccagctgtc ttttatacag gctttttctg aaaagaatac
    14581 aatcagatga tcttagtaaa ccggcatcat tagtaaaata aaaatcatca aactcttcaa
    14641 gaggcgatct aaccaatata ttttctgttt ctggaacaaa gaatttaaag acctctataa
    14701 aatcatcggc tatatcaacc gtgagggggc taatgatcag gatattataa tcttgtcctg
    14761 atatatctct ccatattatt tcatccaatt ttctcgcggg gaattttgga atatcgacag
    14821 caatgagcct gtcgttctga ccaagcttac tctggatttt ttctgtaaga ttcttattct
    14881 gtctggtggg tagaataaca acaaggttca ttttttacct cctgaaaccg gaaatatatt
    14941 tttcttcgcc tgtttgtcat attccctgta tctctgtgtc cttgcatatc cttcattacc
    15001 ccacgacaca agatctttct tgggatcata taacaattca aaggtcattt tggtgggcat
    15061 tacttttatc ataataccac cgcaatcaca cttatgaacc tcttctgatt tggtcacact
    15121 ttcgtagacc tcaatcagtt tattacattt tttacattca aaatcatata ttgggcacat
    15181 ttcttctttt tctcctcatt tttgaatttt tcatatgaaa atctatgtgt attgtgacat
    15241 tatttatctt ctaacaaaag ataggccatt tcaaaattta tcaatttact caaactgtaa
    15301 tggtttgaaa gaagaagggc ggcttctatt cttgctttct ccggttcatt ttcatatttt
    15361 atttgctctt tgaaggcttc cagttcatac atcaaccgga atttatcaaa ataatgatat
    15421 aaaaaaacat tcataccagc agacaaccag tattgtttac aatgtgtcaa ttcatgttgt
    15481 aaaatagcaa tattgccttt atcttccgga cgaatgtaaa taaccattcc cttagatgct
    15541 gctctgacat catccactaa attactttta tagactataa tacaaggcgg acagaagttc
    15601 aattccatcg gtttatcata atcaataagt gatattaggg gattgcttat aaaatagact
    15661 aatattgcgc ctaagaaaac aacacggatt cgaaattgat accacttcat tattacctct
    15721 tagtgtttga tgaatagaac atatggttct gtccaacaaa tcttacaatc tattccacat
    15781 ttgaccttat ttccttttct gcacggacat ataggtacac ccaattcctg ggcccgaatt
    15841 ttaatttctt cttccggccc aaaattaatc ttgccaccaa cgatggaact gacaagattg
    15901 aaattcggaa gtctttttat tttggaccag tcccagagat gctctgtctt ggaataggta
    15961 tagaacatta catcgggtct ttcctttgca atttcatacc acatatccag atattcttgg
    16021 ctgaagaaat ccccgctttc atgtatcctg acaacatcgc ctttctcaag gtgctgaagt
    16081 attctcttct tcagaagttg tgtattttcc tttgcaagcc tgaaatttct ttctctggat
    16141 atacggacta ccggccgcat cttctctgcc tttttggcat aacaatcctt gtagcattct
    16201 ttcatattgg ggcaagaacc acccgcggga agattgaaag ttttaatatt ctttgccagt
    16261 tttgtatttc cccgcatcat agccacccat aaatcctcgg tggggatttg ttctatagga
    16321 acacccctat acttagcggc catttctttt gaatacttct ctccggcagt aacttctttt
    16381 atgtatctgt atagtctcat ttcaacctcc ccgatatatt tatgatcatc ttatctttat
    16441 tttatacaat tcttcccaat aatcttccag ttctgtaacg tgctttggag caaggtattg
    16501 attggaaatc aaccaaagac taaaatccag ataggattta tttttgaacc cctttggatg
    16561 tgggattgtt ttcagaaatg acctgaattt ctcatcttgt aaaacagcct caatggcgtt
    16621 tctcttttgt ctttcataca ttcccattat cagttgttgc gggatgtttt tatcatcata
    16681 agggccaggg atgcctttat aagccttttt attttgcgta taaggggctt ttcctgtttc
    16741 ctgcttacta cctatggcct tttttggcat acttttccag ttctttttaa caagattttc
    16801 aatatatttg gtgattttca tcttgcccgc aagaccggaa tgttctctca tccagttgat
    16861 ataatccaac atagattgtt cacccttcca gcctgggcgt ggaatagcat tcaaaacttc
    16921 cctgaaatct tggttcttgt cgagaaagtc aagcgctatc ctgatccttt ccttctgggc
    16981 atctttgacc tgatttcttt tgaaatccat gacggcttta cggagaagat aatcctcttt
    17041 actgacaggt tccgtggcag attttctact ggccttcatt atacattcgg aaccaacctt
    17101 gaattttttc ttgtctgcac tttcaagcca aaattggaag taaattcccg tagagcagta
    17161 gtcacaacta ccgccgggtt gaactggtgc gccaggatag gcgacgtaga cctctcttct
    17221 tgcgtagaga aatttatatg ggtattttcc aagacccgct tcctcgaaag gatggatttt
    17281 cagattggaa ttttcattca aatatgtttt gagcctcata gagacacact cccaacatat
    17341 ttataaaaaa tggtgcccct gcgaggattt gaaccccgac attatccttg gttcgtggcc
    17401 aagtgctctt tccattgagc cacaggggct tattttttat gattctctaa ccaatgtaat
    17461 tccctatgac aatttgcaca aagaacatcg catttagaaa attcttttat cattcgttca
    17521 tctgaccaac atctataagc catctctgat attcgacaat ctttttttaa tgtgtcttta
    17581 tgataaaaat cgagacaata atatcttact tcaccacact ttaaacatct tttttcgata
    17641 attacgaaca aaagattggg ctgctttttt cctttttgtg gataatacca tcttttgaaa
    17701 aattattttt attaaattca ataagtggtt ttatttcact acatctggaa cactttttta
    17761 taatttaccc cattctggca cccccggcag gattcgaacc tgcatattac tgcttagaag
    17821 gcagttgcct tattccatta ggctacggag gtaaatataa aaaaagggaa cctcacggtt
    17881 ccctctttct ggtgaattca ccgtgatata ttacttgaga accacaatat caactcttcg
    17941 gtctttcgca tggttaccag ttgcttttga cttaccaaat gatttggttg taagtctctt
    18001 actgtcaata cccttcttga taaggatgtt tttcaccgtg tttgctcttt tctgaccaag
    18061 tttcatattg tatttgtccg aaccacgatt gtcacaatta ccctgaagtt ccacggtctt
    18121 tgccgggtca tccttcaggt actcaattgc cctgtcaact gcgtcctgct gtcctttcaa
    18181 tgttgcttta tcaaaatcaa aataaaaaga aagaacatca atcttctttt tcactaccac
    18241 aggtggtgct gggggtggag gaggaagcgg tgttgcttta actacaggaa ccggctctga
    18301 cttacaagaa ggacaatctc ctacaggaac atttgtttta atgggtgtat aacatgccac
    18361 aagtgtcaaa cacaccacaa ttataaggaa attcctcata caattttaac ctccatatgg
    18421 attttttcat ttcataaatg aaaagtttat aatatcttta tatttctccg gtttctttat
    18481 aggaacatag gaagcttcct ctggaaacca cttgaaccat cttgattctt taacgggggt
    18541 atttataccc tctggaacca tctcgataat aaagtaagtc cacatttcca aatcatcgtc
    18601 atagatatgt ttcacaaagt tggacattcc acttgacgca tccggaaagg ttgtgaacca
    18661 tcccaaggga acggtcagaa gaccgttttc attttctttt gccactatta ccgtgaatat
    18721 aactgccata ttttattatc tggtagcggg tcagggtaat gctccctgct gtccaccgtt
    18781 atgagcggtg gatccacctt gtggccccgc ctgcttatcc tttatataat ctactgatat
    18841 ccctattcca tcaaacaagt tggcaatcat atcacacaac tcgtcatcaa attcattggt
    18901 agcgggtgag ggaatcgaac cccctgaacc ttggttatga gccatggcga gataccaatc
    18961 ttacccgcga ttcttgtatt caatatacaa ccagtatatc aaatacgtta taccaactat
    19021 agccacaaga attccaaagg tctttaacga agtccatatc atactgatga gtaataacat
    19081 aatcagaccg ataagaactt tggttcttat gtccaacgca agtattttct cttttagcat
    19141 ctgccaatat atttgtgttt ccaacattag tctttctcca tatctttatt atattgtgac
    19201 actccacgga gcaagccccg tggcttctgc ttagtttact aagcattctt tttcttgctt
    19261 caatgacggc tctattgagc catctccaca agcgttaatt cccgtatgcc ctacggtatt
    19321 caagcctata ttataacata tttatatgag gaaataaaca acttatttat gtcctctacc
    19381 cttttcaaac ccaccaccac gattaaatac tggtggtctt ggttgtgaac gaccaaaact
    19441 atcgaaatgt gggtcttgtc ttgtcatcgg gttacccctg tactgtccgt ggtcataata
    19501 gtgttttggt ggtctgtctc catgacgatg cctgtatgct ggaacataga tgttatgata
    19561 ccaacggtct tgaacaaaat gaactctccg gttacaagca ttataatggt gacaatatgc
    19621 cccccaattt gttatatgaa tagagggaac atataggtaa agaattggca tattgttata
    19681 gtatgggtca tatactgcaa acattggttg tggatagaca cacattggag caggatatcc
    19741 actaccaata tcaatacgtc catagaaacc gggctgacct ataccaattg aaaatcccca
    19801 ttgaacatct gacgcatgag atggaataga aaaacaaagg ataagtagga ttaccagtaa
    19861 aatttttttc atatctgtct tctttgatga tttataccgt ggtggctgtt acaccaccac
    19921 ggtacaataa acacatcacc aattgggttt aaaagttaat tcgggcgata gtaatggttc
    19981 tgaacaggaa taatcacagc accctgcgcc tgaaactgac cggaagcaga agtgaaggaa
    20041 ccatagctaa cggaacttga acaacgatgt ctttcagcat tgaaatagga acctctggca
    20101 aatccaccct gcgcctgaag gttaccacct gcaacaatgg caccaccagt tgtggaaagg
    20161 agttgagcct gaccctgatg accccaaata cttgactgac attgtgacat tgaaacaaca
    20221 ctcatttcct gcggcccaaa tgcaaaggct gacacagcaa aacaaagaac cataataact
    20281 gctaacaaaa tctttttcat atattttctc ctatagtatg ttttttggga aggtcgccaa
    20341 gcgtccgttc cccccgtttt gtataattac ttcatcaaag tattgaatgt agcctcatcg
    20401 gtaagaaccg ttccgtgaat aaacggattg gcttctcttc cggctgaacc ggaagaataa
    20461 ccgataccag ctgttcccgt tccggctctc tgaccgtcat cggaaatacc ggcttgagta
    20521 taaccaagag caacacccca tccggaagtc tttgtatact tctggatgcc ttcaccggaa
    20581 atcagcacat acttggcacc cttgatggaa agggcttcaa gacccatatc accaagaacc
    20641 tgctctgtag tggttccacc agtttttgcc ttagaaacaa tcgaaccaat cttcactaac
    20701 ttcaggtctt tcggaagctc cgtaaggaac gtgatggtat cagtatcggc cggctcggct
    20761 ttgttaccat aaaggttcac aagctgaagc gtctttaccc aaccgtcacc cttcatattc
    20821 tcaaggtgag cacggttgta aacacaaccg aactgaagga tgaacttcac aggctgtgca
    20881 tttgcaaact gtgtgtcatt caagaactca gaaggcattg agggataata cacggtgccc
    20941 ggaatagcga agttacgggg aattggtgca ccattttgtg taatggacac attggccgta
    21001 gattgattac cggacaactc ctgaccctgc atctggcctt gagtctgccc ctgtgcctgt
    21061 ctctggttca tatcaatgtt attcacattg gtattccggt tgctattcct gttataggaa
    21121 ttaccagaat ccctcacggt tgcctgatta ttattcagat taaaggaatt accagaatcc
    21181 ctaaccgtgg cgcttccacc agcaccacca gttgcggtgt tggtgttagt gttagttgcg
    21241 gtggctgtgg cagcaccacc agttgcggta ttggtaatac caccattcac cgtggcagat
    21301 gtaccaccaa actgattgtt gttaccagta ttggcaatac cggagccaat gttctgtgct
    21361 ccatttgcac cagaattggc ctgaccaccg agtgttgccc aagtatctcc actattggca
    21421 aatgccatcg ttgacagggc aagaacaaaa ataaaactca ataaaactct tttcaacatt
    21481 tttactttac ctcctgaaaa tttttgtcca ttttctgttc cccccttctg gaagaattac
    21541 acatcctgtg caaaacatac tatggaacat ttctattatc tactattgtt tttgattttg
    21601 atgctctgac tttcagccgg tacatatgac tgaacaacag aattgttaat tgttgattgc
    21661 ttctcttctt ccatagctcg gttatagatg attttctttt tccgagcatc ctctttgata
    21721 atcttgtcct gttccttaac cttttccttc acgatgaact ccttttcagg gagagacatt
    21781 tgcgcactca ccgtgataga aaaaagattg gggagtaaaa aaacaatgac taccaataat
    21841 acaaattttt tcattatgac ctccttattt gattatatag gtaaacaaac cgtgtgtcac
    21901 aatcagattt ccattgtcat cagtaacatc aatagaaacc ctgtaggtgc cataatcaag
    21961 tggacaactt atgacccacg ttttatcctt atctgatcct ctatgtagaa tggatattcg
    22021 ttttgaaaac tcaaattcgt tgtttgtttt atattcgatt gtttttgcaa gaatatattt
    22081 ggctcccttt ggattgacta acctgaaggt gattgtaatc gccttggttt tgtcttttgg
    22141 aatcctgtac tttttgaaga ggtcaagata ttcgggttca taagattccg gcgaaacttc
    22201 aactgttctt acgatacttt gtactgtctg gaaaccatat gaaggctgtg acaacaccgt
    22261 ttcataattt ggtgcgggca tccctctctc gtctacgact ttgacggttg cgcagcccat
    22321 gactaccatt aaaatcacac acaaaaatac gactttcttc atattccaac cttaaaaaat
    22381 attatacctt aaaaaatatt atttgtaaat atcctatttt tcattttttg atatccattt
    22441 aatatgctct ttataatcct gatgaaggaa ggtatcaatc tcactttcat acaggatacg
    22501 gtctttcttt gagagcttta acaaggcttt tctgaccttt ttctcatagt caagtatctc
    22561 attgaaagcc ttttttctcc tgtcaaaccg gacttcccct gtagtatagg aacgaaccat
    22621 agtccggaga ttacgaatat tcctgcctat ttgagaaccc ttgtcaccac ttgatttcgg
    22681 agtatcataa gtctttgatt tttttactct tttcatattt tatcctatcc tattgaaatt
    22741 gttgagaaat acgaccgttt tatttgactt ataaggggcc tccccatcca tcggtatata
    22801 taaccctcac gcaccgaaaa tccccctata agccaaatgc tccggtttta tcctaattcc
    22861 gaatccatct tctctgcttg tttgaaaagc cagtcacgga tttgaacatc tgtaattttg
    22921 aatccctctt tttccgcggg tgcaataaac tcccacttga agtccatata gagaccgttt
    22981 ttcgaagcgg cctcgatggc ctccttccga cttgacctaa gacctgatcg gacataatag
    23041 tccgttaaaa tcgaaagggc ggtatcagac gggccactac caccgtaacc ccattccata
    23101 ccagtaggac tatggcggac aacctgcttt aagggcaaac ctaaatccag accatcaggt
    23161 tgtctttctt ccacaaggat ggaaaccttg aactcatctt tttcatctct ccaagcaata
    23221 tatctgaact tattcatatt acttttcctt cctcttttta ttctttcctt catattctgg
    23281 cctgtagggt ttaattacaa ggaacatttc accaagttcc aattccagat acttgaactc
    23341 tggtt
//
LOCUS       DAOTQL010000011        23238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2250_length_23238_cov_5.036061, whole genome shotgun sequence.
ACCESSION   DAOTQL010000011 DAOTQL010000000
VERSION     DAOTQL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_23238_cov_5.036061"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..652
                     /locus_tag="P5293_01440"
                     /pseudo
     CDS             1..652
                     /locus_tag="P5293_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925070.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
     gene            652..1782
                     /gene="tgt"
                     /locus_tag="P5293_01445"
     CDS             652..1782
                     /gene="tgt"
                     /locus_tag="P5293_01445"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRR48629.1"
                     /translation="MRYERTVRDTETSASCGLLYTDHGVIETPIFMPVGTVGSVKAVF
                     HRDLIDDIGAQIILGNTYHLYLRPGTDIINRAGGIHKFISWNKPVLTDSGGYQVFSLA
                     ENRKLTNEGCIFRSHIDGSKHFFTPENVVDIQRLIGADIIMALDECPPGSSDHHYAKE
                     SLKLTKQWLERGAVHFDATEPLYNYSQAFFPIIQGCVYPDLRVEAAEHAVSLEREGYA
                     IGGLSVGEPTDIMYQILELLDPILPRDKPRYLMGVGTPVNILEGISRGVDMFDCVMPT
                     RNARNGMLFTSEGVINIRNRKWADDFSLLDENGTSFVDKMYSKAYLRHLFVSSEILGA
                     QIASEHNLAFYLNLINDARLHIKKGDYKMWKQVTIKKLENRL"
     gene            1782..2876
                     /locus_tag="P5293_01450"
     CDS             1782..2876
                     /locus_tag="P5293_01450"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRR48630.1"
                     /translation="MKTNYHIAKLDWYIIKKFIGTYLFILAIIIAIIIIFDISEKIDE
                     MVEKEAPLKEIVFNYYGNMVPYYINLFSPLLVFISVIFFTSKMAANSEIVAILAGGVS
                     FNRMLYPYMISAFFIALLSLVMNLFIIPPANRVRLEFENKYIKDKPYVAGNNIHLQLS
                     PGTYVYIQSFNTADNRASNFTLETIEGRSIKSRLSAESAQWDSTKQCWTLRNYYIRTS
                     LDNRDKIVSGDQIDTVISLKVTDLNQRTNIVESFNIIQLNRYIKTQKMRGDKRVAIAQ
                     IEKHTRFAVPFSAFILTIMGVSLSSKKTRGGIGMNLGIGIALSFIYILFLRFSQMFVH
                     SGIMPPWLALWVPNIVFGVVAYYLYKIAPK"
     gene            complement(2988..3947)
                     /locus_tag="P5293_01455"
     CDS             complement(2988..3947)
                     /locus_tag="P5293_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRR48631.1"
                     /translation="MKILVVGAAGQIGTELVPHLQKLYGEDNVIAADIKTDVVKKLSN
                     TSRALVLDATDPSNYTEVVRQHKVERIYNLVALLSATGEKNPQLAWRINMGALINSLE
                     IAREFNCALFTPSSIGAFGKSTPHVKTPQDTIMRPNTMYGICKVSGELLSDYYFERFG
                     VDTRSVRFPGIISSGTLPGGGTTDYAVEVFYEIIKKKSYTCPIPANSFMDMLYMPDAL
                     KACTDLMDADPSKLVHRNSFNITAMSFSPEILFAEIKKHLPEAQFNYQVDPVKAEIAD
                     SWPDSLDDTCARAEWGWKPEWYLENMVTDMLRAIRDKFNSQSI"
     gene            4080..5003
                     /gene="queG"
                     /locus_tag="P5293_01460"
     CDS             4080..5003
                     /gene="queG"
                     /locus_tag="P5293_01460"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRR48632.1"
                     /translation="MNSLVKTWAEELGFVACGFVRARKMTETLEPLKEYIARGYNADM
                     GYLERNIEARIDPSLLLPGVKTLLVFLAPYRQKESQPEGLPKIATYAWGLDYHIVIRN
                     KLYEIVRRIRNIVPELKARVFTDSAPILERLWAKEAGLGFIGKNGFLISREHGLHNLI
                     GIILVDVEMDYSLSLNIKGGCGTCRRCLDACPSGAIVEPFVVDSRKCISYQTIESKKK
                     FCDEEYRIDRKGYIFGCDICMDICPWVNRAKYSEWNEFAPLFSEKTGKKIVEFSKEEW
                     MALSEEYFAELFINSSLKRAGLSKIKDNLEP"
     gene            5013..5945
                     /locus_tag="P5293_01465"
     CDS             5013..5945
                     /locus_tag="P5293_01465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRR48633.1"
                     /translation="MKFFTKVNVEDLNCRISYKDKIISLGSCFANEIGAKLKDSRFDI
                     LVNPFGVLYNPASIASALERLSSGNVFSEEDIFTDGDLWSSFYHDSLHAEYTKEALIN
                     KINTSLKSDSMHFITSSWILLTLGTRRVYTLKKKNIIVSNCHKLPQSEFVIGQLKIDD
                     IIRILAPYIEANLNKTWLFTVSPVRYLKDGAHDSQISKATLLLAVEELTKLFPNVIYF
                     PAYEIYMDELRDYRYYADDLIHPSQSSLKYIWEIFRDSLINKEDFYLLDMVERLNDMK
                     KHHPFFSESKAFKKFEEEMLQLEEIIDKQFKEKM"
     gene            6033..6302
                     /locus_tag="P5293_01470"
     CDS             6033..6302
                     /locus_tag="P5293_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRR48634.1"
                     /translation="MNKAELISAIASKANITKVDARKALDAFVESAGEAMKNGDRLTL
                     VGFGSFSVAKRSARNGRNPRTGKTIKIPAKNVVRFKAGADLNKLV"
     gene            6557..7090
                     /locus_tag="P5293_01475"
     CDS             6557..7090
                     /locus_tag="P5293_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR48635.1"
                     /translation="MINHHILIGDKKYEFKEIFKDFFPSQVIFASKIVKDDHLAEDVV
                     QEVFLKLWRSEAFFQNEISFKAYLYLSTRNRCIDFLRTKKIKTGNIELGLNVEEEANI
                     VLKEEAFRLLDKAIANLAPQSQKIIKLSMQGFSIQEISEKLSISVNTVKTLKARAYKT
                     LRNHLGEIFILMLSPFL"
     gene            7143..8264
                     /locus_tag="P5293_01480"
     CDS             7143..8264
                     /locus_tag="P5293_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRR48636.1"
                     /translation="MEEKYKKAIEEASSLADKMLSEPARFASEFNIRRKHLAKINVEK
                     KWIIFWFTVNYKKIISVSAAAAAVIILSSILFNLNTNSIDEGKLRSQINIMPATGSVV
                     LKLASGELVELDSAVNVQNKLLGVKVDVGNSVISYADDSASLTASGIQNIFKLKYNQY
                     NQLSVPKGRSYSVILSDGTKVYINSMSKLYYPVEFNGTERRVKIEGEAYFEVTKNEKK
                     PFVVETKSYSVKVLGTKFNINAYPEEKLVTTTLISGLVEINEGKDVSVKITPGKQLRM
                     DKETKQISVEEVDTELYISWIDNVLRIQDTRLEDILNILKRRYDVDIKIEDYSVLNEK
                     FTGKVPLNDNLLVILEQLEKVSQINFTINERTITVRRNT"
     gene            8433..11324
                     /locus_tag="P5293_01485"
     CDS             8433..11324
                     /locus_tag="P5293_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRR48637.1"
                     /translation="MKQIEKQTGYGYLFKDENIKSIKGISYTAENEDILTVLKGLLAN
                     TGYSFELNNKVILIFKEVKPETTLRQVAVPKEVPVKFKIVDSLTNEPLIGATCNLKNY
                     SIYGTADIDGNVFLKNVPTGDAHIDIQMLGYEPKTISMKIISPVEMRIALIPTSLELD
                     EIMVVAKASTAGTSTSSMIGRQAIDHLQATSLKDIMQLIPGQLVTGVTDMTSASKITI
                     RTLNTSSNNAFGTSIVVDGIPISDNATLRDKTAINATGATGVDLRQIEADNIESVEVI
                     RGIPSAEYGDLASGAVIVNTKSGYTPYEIRAKINPKNFNTSVGKGWTLGNKQGALNAS
                     LDYMQAWGDPRQKTSSFDRISAGLSYSRKIGKIWYTTTKLSVSSLIDSRGDDPDVITE
                     GTETTQKNMNFRLSHNGKISVNAKLMRTLSYYLGYSESITESKNSIIVAAGGGMPVIT
                     SLTSGYFEVPYITDSYRASGTTEGRPRTFYAKVSNGFTANVEKFRQRFNMGVEYKWEE
                     NKAKGYYNDDDSKPLRPNSDGRPRPYYDIPALNQISAYFEDNIDWKISKSLAFKVQAG
                     LRYNLIQPGIEEQVSSFSPRINSSIHISNWLEIRAGWGLNSKTPGLSHLYPEPKYTDR
                     IAARYLPAGQNEQLVMYHTNVNYVERNNALKNATNSRTELGVDIKLVNGMRFSVVGYN
                     DYMEDGFGNLTEYKIYYSRYYDANKGLIIKPEMKPFVDWNNPARVDTVFITTGRIGNT
                     EASIDRGIEFDFDLGEVKVLRTSFYLSGAYIQSKYWDNGRNFSNPVGIPAGSVYSQGG
                     SNTPPFKLEYPSGRSKNINERFSTLIRTVTHIPYLKMVATLTGQIVWYSSAETTNQKQ
                     DPIGWIDTDLSYHPITQQMLDDPSCKIKGILLSDQRKDPKDDGATRQPLIWIINARLS
                     KDVSKSLGFSFFVNNLFYYTPYQSTNKSGTLTERNTDTFSFGMELLIKI"
     gene            11327..12556
                     /locus_tag="P5293_01490"
     CDS             11327..12556
                     /locus_tag="P5293_01490"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HRR48638.1"
                     /translation="MKKHKIIQILNSVILSIILISCTREEPLPIHSVHIAVNLPANFR
                     ADVKFANKEVILTSSSNEYRFITNAQGEVIVNDLIPDVYNIIAQWEMSGVEYKQLIAN
                     PEPVEDRATVFLNVALNNYKIFSDVNITLNLEAVILKSLLISKIYYTGTKDNANKNYS
                     ADSYIEIFNNSEDTVFLDGKYLALTESMSPAAYPAKDNPDFIYARQICKFPGDGNDYS
                     VLPSTSIVIAARSARDHRLSASTSVDLSNADFEVKDADGSGNPIIKALPIISNSIPAL
                     KFLNLLTGGGNGVFIFETNEDISSWPEVYAPGKTSGERFRKVPVGVVLDGVECLKNNV
                     GTGPDINLKRLPFVIDAGYTFVNATSGYTHESLERKVIIRNGVLKLVDSNNSIEDFVI
                     VLDPTPKKYDKPELMNN"
     gene            12560..14143
                     /locus_tag="P5293_01495"
     CDS             12560..14143
                     /locus_tag="P5293_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48639.1"
                     /translation="MRNIYIIIVLFFMTTVNIAVGQEIDSVAIEAPVKLKERELQIFN
                     QQWNITENAAGLGINKISNSSLTTIGLSRSTGDFHRAQEGSSFNRLEFNTDRFDKFND
                     QLYLKGDFSFNLEKEFNRAWSDVFHTYNSSPYIFGSSVEGDYETQRFRISFMIFTALN
                     GRFNYGIGVDYKVADMSRQKDPRSRSYLLDYKIIPSITYAINQKNILGINCSYRFEKE
                     KMPNVTTVQTDPNLKYYDFKGLNYVDGKIGGYKGFQRQFISDIPGVAIQYNFLGVSSK
                     LLSSIGVEYQEQQILGNKKQSPGSYNSFNYSLVVDYVVNSQDYIHNINLKGSFRDGGA
                     DEFRQELKSSKDPITGETTETWVTNYKYKNRYMVKVADVYLSYKLMKQTSDKKSIKWF
                     IQPYAAFNKFSNTYYLPKSEYVVSKFFAGTNAFLSIYSRNNSDVDLNFNVAGGFPVKT
                     DLSLVTVSELNSAIYDIDIEYHKKKTIETYGEIRYTFPIKLGKNKMIGYSRLYAGNIF
                     GIGSSSWFSTGFSIGIITL"
     gene            14145..15626
                     /locus_tag="P5293_01500"
     CDS             14145..15626
                     /locus_tag="P5293_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRR48640.1"
                     /translation="MMKKKLCGLLTLIAGFLVIQSCEKIEDTRSYAPVLFFEKSTETV
                     KTDVGSYDVKLVLSKPAEKEIKAKIGFTGTAIEGEHYSVASNEIVFAKGDKEAFLKIN
                     ILNENIYDELINIKCLICPSQEYAVIPELKSEFILNMTKDIILPTISFKSTEQNRFTN
                     PYLAETLTYELCLTEALRMDTEVRISVEGDLSIGADFLINEGNSNTLIIPKSVLSKIF
                     TVTFKKKDMAGYNRTLKLTLVPVTSKSCIVNEESSFTNITVKDPVVDMSSFFKTAALL
                     GGPGYQMNQAIKAKDGTYSSNIVVNVLNNSSKGNYMKSLRNLSFNSAFGCYANTPGGD
                     VLRLADLLKFNTTDTVIADYGVGKTTRFFSPSDSLLRFVAEGDNIRKGKVFAPAQKFS
                     AKLVLKQDWETGTNGNKQWHLDSKSTGGNISLSSYPLSFATMEIELVKLEGTFNIDED
                     IPVIIFDAWFRSNSKYFMRNIPESYDIVREGDLYRISYKFTPR"
     gene            15659..17926
                     /locus_tag="P5293_01505"
     CDS             15659..17926
                     /locus_tag="P5293_01505"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HRR48641.1"
                     /translation="MLLIFHVAIPAQGHFIKICNTNGYKFEIPDYVIGKDILFGSRIV
                     DISKPEAKVYAAGQMRKPPVIIRFTKSGNTLRMDQIINFYKIDKNDPIYESVEKNQRV
                     SAVYTFDIEQKVRKDGSLVNVIDVSKFFLDEVTIAWPLPDNVNNGRLDSKLTSVLFMK
                     ELDDHVNIRVYYGYTGGKEPFSITVQYFMLLLPELLKPRYDDDRVGYQTISLKKYESG
                     KVISKEKIISRWRIEPKLSDVEKHRKGQLVEPENPIVIFIDPAFPSDWIPYIKMGIED
                     WQKAFEKIGFKNAIIAKEYPKNDTDFDPYDISVNCVRYIPVQEANASGQIWIDPRSGE
                     ILQGDILWWNDVISLIKMWRFTQTAAVDPEVRRPFFSKEIEGELIRYAIAHETGHMLG
                     LQHNMRASYAYPVDSLRSPTFTMKFGTTASIMDYARNNYVAQPGDLQRGVKMTPPALG
                     PFDFLSIEYGYKIIYGADTEKKEREELEKIFISKGDDPMYFFGPLTMSQVFPDPASQS
                     DALGDDIIKSSYYGIKNTRIILENLYKWTIDTGGDESLLVERYNALINQYYRLLSLPL
                     SYVGGKYQFYGNLLAEKPKYIPVEKQKQIEAVNYITLELKEAGKHTLRRELLPLIGSE
                     YVDKVYKKEADLVALMVSPIILTRLVQSSFFDYSCLSIEEYLDMIDDNILNPSYDYNN
                     PFVKNIQTSYIQALISVYKQQGGEIINGSNAIVLESIFDRIIKTRSKLTNLINKQSGN
                     SGYTKYLLSLLKTDK"
     gene            17933..20092
                     /locus_tag="P5293_01510"
     CDS             17933..20092
                     /locus_tag="P5293_01510"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR48642.1"
                     /translation="MKRILITLITVVFFVPFAIADEGLWLPFLLKDQKFSQMRKMGLK
                     LSAEEIYSVNQACIKDAVIGLMSEGSNLRSYGTASFISDKGLIITNYHVILPYIQRFS
                     TQENDFIKYGFWAKNSSEEIVCRGLEMKQLIRMEDVTEEILNGTKGLQGQEKLNKINS
                     NGKKIAEEVTRGTKYEVSIQSVFGNNQYIMSLYIVYKDIRMVVVPPFALGKFGGNTDN
                     YSWPRHTADFAILRVYADQNNEPNNYSKTNGFYKPRKSLVLSTSGFKEGDFVMILGYP
                     GKTRQYIPSFALDEIINVEGPARMDLANTKINILKQAVAENPEMKYRYTAKLSSEGNI
                     YLRTKGQMQGVRRTNLIDRKKEEERNFIEWANTSLIKDMEELYKEVAIYNLADLYFKE
                     AGINGAEIVPFIGKFEKLVSIYSRKHFDKIVADRECKRLKGLTDQFFESWSYEVDRKI
                     FSNLMLKYYQNLPDKFHPVSMVKYMKYYQSDVEELSKDVFRRSIFTDKDRLMAFLDDD
                     RKNVDSLVKADPLYQLSIGYYMANVEKIANQRAQLQAKQLEMFEKYMEGFIEMNDNKI
                     LFPDANNSQRLSYGKVRGSTAEDGLIYTSYTTLDGMHEKYLNNISEEEFYLPVKIQKL
                     YKEKDFGKYGDKKGNITVNFLTDCHTSGGSSGSPVLNARGEMIGLNFDRIWQGLVSDY
                     KFEPEQSRSIAVDIRFILFIIDKYSPSSYILEELKIK"
     gene            20239..21378
                     /locus_tag="P5293_01515"
     CDS             20239..21378
                     /locus_tag="P5293_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRR48643.1"
                     /translation="MDFSLSKEQLLFLKMIREFAEKEVKPLAAEIDEEERFPVETVEK
                     MAKLGIMGIPFPVEYGGAGGDNMLYTMAVEELSKFCGTTGVIVSAHTSLCAAPIYEYG
                     TEEQKKKYLPKLCSGEWLGAFGLTEPNAGTDASAQQTTATLDGDYYILNGNKIFITNA
                     GYAKVYIVMAMTDRSLGTRGISAFIVESDVPGFSVGKKEKKLGIRGSSTSELIFENCR
                     VPKANLLGKLGGGFGIAMKTLDGGRIGIAAQALGIAQGAMDETVKYVKERKQFGKPLS
                     QFQNTQFQLADLNTKIEAARLLVRCAAFKKDQKQNYSTEAARAKLFASETAMEMTIKA
                     VQFHGGYGYTREYPVERMMRDAKITEIYEGTSEVQRMVIASSLLK"
     gene            21389..22174
                     /locus_tag="P5293_01520"
     CDS             21389..22174
                     /locus_tag="P5293_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRR48644.1"
                     /translation="MNIVVCIKQVPNTTEIKLNPETGTMIREGVPSIMNPDDKSGLEL
                     ALKLKDNFGAHVTVITMGPASADDILREAFAMGADRAILLTDRKFAGADTLATSRALA
                     GALRKMDHDLIITGRQAIDGDTAQVGPQIAEHLDIPQVTYVEEFNYTAPNYFKVKRTI
                     EEGSQIVEVETPCLMTVVSSAANKPRYMTVGGIINAYRRHVDIWGCADINVPEELLGL
                     KGSPTRVWKSFTKGARGAGKIYEVEPAKAVEIIIEKLKEKFII"
     gene            22188..23183
                     /locus_tag="P5293_01525"
     CDS             22188..23183
                     /locus_tag="P5293_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRR48645.1"
                     /translation="MDKSQYKDIYVFIEQRDGIVQNVSFELLTKAAELASVLKEKVYA
                     MLLGHNIATKSQDLIAYGADNVLCVDAPELEEYSTEPYAQAVAQIINERHPSIVLIGA
                     TTIGRDLGPRLSARIGTGLTADCTGLDISEDGNLLMTRPAFGGNLMATILCKDHRPQM
                     STVRPGVMLKGARDDNRKGIEERININFDKSKFRVRIIETVKEEKNRIDITEAKVLVS
                     GGRGVGDAEGFERLAKLAEVIGAEVSSSRAMVDAGIIPHERQVGQTGKTVRPDLYFAI
                     GISGAIQHLAGMEESGYIIAINKDKNAPIFQTADLGIVGDESKIIPLLTEKLSKK"
BASE COUNT         7723 a         3501 c         4689 g         7325 t
ORIGIN      
        1 tcgggcttga tgcacttgtg ctttttaagc agcaaaaaac agcagtgttt tttctatttg
       61 ccatgttgct tggagcagca cttcagataa ccaacacatt tggatcacca ttcttacagg
      121 attttgccaa tgaccctaaa tttgttgatt catttgcagt gaagcatccg ggtattttga
      181 tgtcaatatc tcagatgtct gagactcttt tcattcttgc aattccgttt tttctaaaac
      241 gttttggcat caagatagtc gttttaatga gtatgtttgc atgggtactg agattggttt
      301 ttttgggatc ggcaatcctt ctgaaggttt tgtgttcctc attttatcga tgattgttta
      361 tggaatagca tttgactttt tcaatatttc cggttcactt ttcatagaac aggagactaa
      421 acctgaaata cgcgccagtg ctcaaggtct ttttatggtt atgacaaatg ggattggagc
      481 cacagttgga gggttaacct caggttgggt agttgacatg tttacgtctc cgggtggaat
      541 acgtgactgg agtactatct ggtttgtatt tgcaagttat gctcttgtta taggcattct
      601 atttgccatt ttctttaaat acaagcataa tccccagcct gtagcagagt gatgagatac
      661 gaaagaacag tcagggatac cgaaacttct gcatcatgtg gtttgcttta tacagatcac
      721 ggcgtaatag agactcctat ttttatgcct gttggtacag tcggaagtgt taaggcagtt
      781 ttccacagag atcttattga cgacataggt gctcagataa tacttgggaa tacttatcac
      841 ttgtatcttc gtcccggtac tgatattatc aacagagccg gtggaattca caagtttatc
      901 tcgtggaata aacctgtttt aactgatagt ggtggttatc aggttttttc tctggcagaa
      961 aacagaaagc taactaatga aggttgtata ttcaggtctc atattgatgg atcaaagcac
     1021 ttttttacac ctgagaatgt ggttgatatt caacgattga taggggctga tataattatg
     1081 gcgctggatg agtgtcctcc gggcagttca gaccatcatt atgcaaaaga atcattaaaa
     1141 cttactaaac agtggctgga gagaggggca gtacatttcg atgctacgga acctctttac
     1201 aattacagtc aggctttctt cccgataatt caaggctgtg tatatcctga tctgagggtt
     1261 gaggcagctg agcatgcagt atctctagaa agagagggat atgcaatagg aggattgtct
     1321 gtaggtgaac ctacagacat tatgtatcaa attcttgagc ttttggaccc gattttgcca
     1381 agagataaac ctagatatct tatgggtgtt ggtaccccgg tcaatatctt ggaagggatt
     1441 tcgagaggtg ttgatatgtt tgattgcgtc atgcctacaa gaaatgcaag aaatggaatg
     1501 ctttttacat cagaaggagt aatcaatata agaaacagga aatgggctga tgatttttct
     1561 ctattagacg agaatggaac gtcttttgtt gacaaaatgt attccaaggc atatttgagg
     1621 catctttttg tttcttctga gattctgggg gcacaaatag caagcgaaca caatcttgcc
     1681 ttttatttaa atttgataaa tgatgctcgt ctacatataa aaaaggggga ttataaaatg
     1741 tggaagcaag tcacaatcaa gaaacttgag aaccggctct gatgaaaact aattatcata
     1801 ttgccaagct tgactggtac ataataaaaa agtttattgg cacatatctt tttattcttg
     1861 ccataatcat tgccataatt atcatttttg acattagcga gaagattgat gagatggtgg
     1921 aaaaagaggc tcctcttaag gagattgttt ttaactatta tggtaatatg gtgccttatt
     1981 acataaatct cttcagtcct ttactcgtct tcatctctgt tatatttttt acctctaaga
     2041 tggcagctaa cagtgaaatt gttgctattc ttgccggagg agtcagtttt aacaggatgc
     2101 tttatccata catgatttca gcttttttca ttgcgcttct aagccttgtg atgaatctct
     2161 ttattatacc tcccgcaaat agggtgagac ttgagtttga aaataaatat attaaggaca
     2221 aaccatatgt tgcgggaaat aatattcatc ttcagctttc accaggtaca tatgtttaca
     2281 ttcagtcatt caatactgcc gacaaccgag cttcaaattt tactttggaa actattgagg
     2341 ggcgttcaat caagtcgaga ttaagtgcag aaagtgctca atgggattcc accaaacaat
     2401 gttggacact tcgtaattat tatatcagaa ctagtctgga taacagagat aaaattgtat
     2461 ccggtgatca aatagatact gtaataagtc tcaaggttac agacttgaat cagagaacca
     2521 atattgtaga atctttcaat ataattcagc tcaacagata tataaaaaca cagaagatga
     2581 gaggagataa aagagttgca attgctcaaa ttgagaaaca tacccgattt gctgtgccat
     2641 tctcggcttt tatccttacc atcatgggcg tttctctatc ttcgaagaaa accagaggag
     2701 gaataggtat gaatctcggc attggtattg cactaagttt tatatatatt cttttcttga
     2761 gattcagtca gatgtttgtg cattccggga ttatgccgcc ttggcttgcc ctttgggtgc
     2821 ctaatattgt atttggagtt gtggcttatt atctttataa aattgcacca aaataatttt
     2881 gcatcaataa tgtatgtgca aaattatttt aacatgcttg actcaaaaaa tacgaaatat
     2941 atgtagggga tgttttatga tttctgcaat taagctgtcc agtattatta tattgattgg
     3001 ctgttaaatt tatctctaat tgccctgagc atatcagtta ccatattttc caagtaccat
     3061 tcaggtttcc aaccccactc tgctcttgca caagtgtcgt caagggaatc cggccacgaa
     3121 tctgcaattt cagctttgac aggatctacc tgatagttaa attgagcttc aggtaaatgt
     3181 ttctttattt cagcaaagag aatttcagga gaaaaactca tggctgtaat gttgaaactg
     3241 tttctgtgaa caagtttgga tggatcagca tccataaggt ctgtgcaggc tttaagtgca
     3301 tcgggcatat agagcatgtc cataaaggaa tttgccggaa tagggcaagt ataactcttt
     3361 tttttaataa tttcatagaa gacttctact gcatagtctg tagttccccc tccgggaagg
     3421 gttccacttg aaattatgcc ggggaatcgg acacttcttg tatcaactcc aaatctttca
     3481 aaataatagt cactaagaag ttcgcctgat actttgcata tgccatacat tgtatttggt
     3541 ctcataatag tgtcttgtgg tgttttgaca tgaggagtgg atttaccaaa agctcctatt
     3601 gaacttggag taaaaagtgc acaattaaat tctctggcaa tttcaagtga atttatgagt
     3661 gctcccatgt taattctcca tgcgagttga ggattttttt ctccagtggc agataagaga
     3721 gcaacaagat tgtagattct ttcaactttg tgttgtctta caacttcagt gtaatttgaa
     3781 ggatctgtag catcgagaac taatgctctt gaagtgtttg acagtttctt aacgacatct
     3841 gtcttaatat ctgctgcaat tacattgtct tcaccatata atttttgaag gtgaggaacc
     3901 agttccgtgc ctatttgtcc agctgctcca acaactaaga ttttcatacc tttgtatttg
     3961 tgttatattt tttgatttaa taaatgctgt aagtgaaacg ctggcaaatg tatgaaattt
     4021 ataccaaatt tggctttttg attacacttt ttaacatatt aatttaacaa tctaacttaa
     4081 tgaatagtct agttaaaaca tgggcggagg agcttggttt tgtggcatgt ggatttgtca
     4141 gagccaggaa gatgacggaa acattagagc cacttaagga gtatattgcc aggggttata
     4201 acgctgacat gggctatttg gaaagaaata ttgaagcgag aattgatcct tcacttttat
     4261 tacctggagt aaaaactttg ctggtttttc ttgctcctta caggcagaaa gagagtcagc
     4321 ctgagggatt gccaaaaatt gcaacctatg cttggggctt agattaccat atagttataa
     4381 ggaataaatt atatgagatt gtaaggagaa taagaaatat agttccagaa ttgaaagcaa
     4441 gagtttttac agattctgcg ccgattcttg agcgtttatg ggcaaaagag gctggtcttg
     4501 gttttattgg caaaaatggc tttcttataa gcagggaaca cggcttgcat aatctaatcg
     4561 gtattattct tgttgatgtt gagatggatt attctctttc tttaaatatt aagggaggtt
     4621 gcggtacttg tcgtcgatgt ctggatgctt gtccttctgg agcaattgtt gaaccatttg
     4681 ttgtcgattc taggaaatgc atctcatatc agacgattga aagtaaaaaa aagttttgtg
     4741 atgaggaata tcgtattgac agaaagggat atatttttgg ttgtgacatt tgcatggata
     4801 tatgtccatg ggtaaatagg gcgaaatatt cagaatggaa tgaatttgct ccactttttt
     4861 ctgaaaaaac aggcaaaaaa atagttgaat tttcaaaaga ggaatggatg gctctttcag
     4921 aagaatattt tgctgagtta tttataaatt catcgctaaa aagggctggt ttgtcaaaaa
     4981 ttaaagataa cttagaacca tgaatatata atatgaaatt ttttacaaaa gttaatgtcg
     5041 aagatcttaa ttgccgaatt tcatataaag acaagattat ttcattggga tcatgttttg
     5101 ctaacgaaat aggggcaaaa cttaaagatt cacgctttga tattcttgtt aatccctttg
     5161 gagtacttta caatcctgca tcaatcgctt cggccttaga aaggctctct tcagggaacg
     5221 tcttttcaga agaagatatt tttaccgatg gagatttgtg gagtagtttt taccatgatt
     5281 ccttgcatgc agaatataca aaagaggcac ttatcaacaa aataaatact tccctaaaaa
     5341 gtgattcgat gcactttata acttcttcat ggatactact cactttggga acaagaagag
     5401 tatatactct caaaaagaaa aacattattg tgagtaattg tcataaactt ccacaatccg
     5461 agtttgtaat tgggcaactg aagatagatg atattataag gattttagct ccttatatag
     5521 aggctaatct caataaaaca tggcttttta ccgtaagtcc cgtgagatat ttaaaagatg
     5581 gtgctcacga cagccagata agcaaggcaa cattgctcct tgctgttgag gagctgacaa
     5641 agttgtttcc aaatgtcata tattttccgg cttatgaaat ttatatggat gagttgaggg
     5701 attatcgata ttatgcagat gatttaattc atccttctca gagctcttta aaatatattt
     5761 gggaaatttt cagagattct cttattaaca aggaagattt ttatctgctt gatatggtgg
     5821 aaaggcttaa tgacatgaaa aaacatcatc cttttttttc tgaaagtaag gcttttaaga
     5881 aatttgaaga agaaatgttg caattagagg aaattattga caaacaattc aaagaaaaaa
     5941 tgtaactatc tgacaaataa tattgttttt tattaattaa aaatttaatt ttgcatcaat
     6001 tgcaagagat tcaacaacct aaaaacttaa ttatgaataa ggcagaatta atttcagcta
     6061 tcgctagcaa agccaacatt actaaagttg acgctagaaa ggcattggac gcatttgtag
     6121 aatcagccgg ggaggccatg aaaaacgggg acagactgac acttgttggt tttggttcat
     6181 tttcagtagc taaaagaagt gccagaaatg gcagaaatcc tcgaacaggc aagacaatta
     6241 agatccctgc taagaacgtt gtaaggttta aagccggagc agacctcaac aaacttgtct
     6301 aagtgttttt gtctccaatg tgagattttt gaaagagggt ggccaaaaat tactttttgg
     6361 ccaccctttt tttatttttc aaagcctcca cctgtcggag tttgtcgtaa acggctcatt
     6421 aataaatatt tgatttttta gattttaaaa agcctaattt ttaaaacact aattacaagc
     6481 catttacgac aaactccgac aggtggagtg cctattttaa tttttacttt atatttgagc
     6541 tccgttataa agctaaatga tcaaccatca tatattaata ggcgataaaa aatatgaatt
     6601 taaagaaatt tttaaggatt tctttccttc tcaagtaatt tttgcctcta agatagtcaa
     6661 ggatgatcat ttagcagaag atgttgtgca agaagttttt ttaaaattgt ggagatcaga
     6721 agcatttttt cagaatgaaa tatcttttaa agcttatctt tatttgtcta ccaggaatag
     6781 gtgcattgat tttttaagaa caaaaaaaat aaaaactgga aacattgaac ttggattaaa
     6841 tgtagaagag gaggctaata ttgttcttaa agaagaagca ttcaggctat tagataaagc
     6901 tatagctaac cttgctccac aatctcagaa gattataaag ttgtctatgc aaggattttc
     6961 aattcaggaa atctcggaaa aattaagcat atctgtaaat actgtaaaaa cgcttaaagc
     7021 cagggcttat aaaacgttga gaaatcattt aggtgaaatt tttatattga tgctgtcacc
     7081 ttttttataa aatgtccgtt ttatatataa ggaaatgttt aaaggcgttt tcttaatatt
     7141 taatggaaga aaagtataaa aaagcaatcg aagaagcaag ttcactggca gataaaatgt
     7201 tgagtgaacc tgcaagattt gcgtcagaat tcaatataag aagaaaacat ttagccaaaa
     7261 taaatgtaga aaaaaaatgg attatttttt ggttcactgt caactataag aaaatcattt
     7321 ctgtatctgc tgctgctgct gctgtgatta tattaagtag catacttttc aatttaaata
     7381 caaatagtat tgatgagggg aaacttagaa gtcaaatcaa tattatgcct gccactggtt
     7441 ctgtagtcct aaaacttgca tctggtgaat tggttgaatt ggattctgct gttaatgttc
     7501 aaaacaaact tttaggagtt aaagttgatg taggtaatag tgtgatttct tatgcagatg
     7561 atagtgcatc tttaactgct tctggcattc aaaatatctt taaattaaaa tataatcaat
     7621 ataatcaact ttcagttcca aagggacgct catattctgt aatcttgagt gatgggacta
     7681 aagtatatat taattccatg agcaagttgt actatcctgt agaatttaac ggtactgaaa
     7741 gaagggtcaa aattgaagga gaggcttact tcgaagtgac caagaatgaa aaaaagcctt
     7801 ttgtggtgga aactaagtca tactctgtca aggttttagg tactaagttt aatataaatg
     7861 catatccgga agagaaatta gtcactacca cattaatatc cggcctggta gaaattaatg
     7921 aagggaaaga tgtgtctgtt aaaattactc ctggaaaaca attaagaatg gacaaagaaa
     7981 caaagcaaat atcagttgaa gaggtggata cggagttgta tatttcatgg attgataatg
     8041 ttttgagaat acaggacaca agacttgagg atattcttaa tatacttaaa agaagatacg
     8101 atgtagatat aaaaatagaa gattactctg ttttgaatga aaaatttaca ggtaaagttc
     8161 ctttaaatga caacctttta gtgattttgg aacagcttga aaaggtttct cagataaatt
     8221 ttacaattaa tgaaagaacc attacagtga ggagaaacac ataaataact ggttatttga
     8281 ctaattaatc ccaacaataa gaaactatga aaaatggaat ccggctatta ttgcattttg
     8341 caatgatttt gtttttgcta acagtatgga caacggttag tgcacagcaa atcaaatcat
     8401 taaaagtgca aaatgcttca ttagaagatt gtttgaagca aattgaaaaa cagacaggtt
     8461 atgggtatct ttttaaagat gaaaatataa aatcaataaa aggtatatcg tatactgctg
     8521 aaaatgagga catattaacg gtgttgaaag ggttactggc gaatacggga tattcctttg
     8581 aacttaacaa caaagtcatt ttgatcttta aagaggtaaa acctgagaca actttaaggc
     8641 aggttgctgt tccaaaggag gtgcccgtta aattcaaaat tgtagacagt ttaacaaatg
     8701 agccattgat tggtgctacg tgtaatttga aaaattacag tatttatgga actgcagata
     8761 ttgatggtaa cgtttttttg aaaaatgttc ctactggaga tgctcatatt gatatacaaa
     8821 tgcttggtta tgaaccaaaa accatatcga tgaaaattat ttcaccggta gagatgagaa
     8881 ttgctcttat tccaacttca cttgaactgg atgagattat ggttgtagcg aaagcgagta
     8941 cggcaggaac ttctactagt tcaatgattg gccgtcaagc aatagatcac ttgcaggcaa
     9001 ccagtttgaa ggatataatg caactaattc ctggacaact ggtaaccggt gttacagata
     9061 tgacaagtgc ttcaaagatt actattagga ctcttaatac ctcttctaac aatgcatttg
     9121 gcacctcaat agttgttgat ggcatcccaa tttctgataa tgcaacgttg cgagataaaa
     9181 ctgctataaa tgccacagga gcaacaggag ttgatctaag acaaattgag gcagataata
     9241 ttgaatcagt tgaagttata aggggtatcc cttctgcaga atatggggac ttggcttcag
     9301 gggcagttat tgttaatact aagtctggat atactcctta tgaaataaga gctaagatta
     9361 atccgaagaa ttttaacact tcagttggta aaggatggac tcttggcaat aaacagggag
     9421 ctttgaatgc tagcttggat tatatgcagg cctggggtga cccccgccag aaaacatctt
     9481 cattcgatag aatatctgca ggtctttcat atagtaggaa aattggtaaa atttggtata
     9541 caactactaa actctctgtt agtagtctta tagattcaag aggagatgat cctgatgtca
     9601 ttactgaagg cactgaaact actcagaaaa atatgaactt tagacttagt cataatggga
     9661 aaattagtgt caatgcaaaa ttaatgcgta cactttcgta ctatttgggt tatagtgagt
     9721 ctataacaga gtctaagaat agtataattg ttgctgcagg tggtggaatg ccagtgataa
     9781 catccttgac ttctggctat tttgaggtcc cttatattac cgattcctac agggcttcag
     9841 gcactacaga gggtcgccca aggacattct atgctaaagt gtctaatgga tttactgcca
     9901 atgtcgaaaa atttcgtcaa cgatttaata tgggagtcga gtataaatgg gaagaaaaca
     9961 aggctaaggg atattacaac gacgatgatt ctaaaccatt aaggccaaac agcgatggcc
    10021 gccctagacc ttattatgat atcccggctt tgaatcagat ttctgcttac ttcgaagaca
    10081 atattgactg gaaaatatct aaaagtcttg cttttaaagt tcaggcaggt ttaagatata
    10141 atttgattca acccggaata gaagaacagg tgtcatcatt ctctcctaga atcaattctt
    10201 ctattcatat ttcaaattgg ttagagatta gagcaggttg gggactcaat tcaaaaactc
    10261 ctgggttgtc acatctttat cctgaaccaa aatacactga cagaatagca gcaagatatc
    10321 ttcctgcagg ccagaacgaa caacttgtca tgtatcatac aaacgtcaat tatgtggaaa
    10381 ggaacaatgc acttaaaaac gcgacaaatt caaggacaga gttaggtgtt gacatcaagc
    10441 ttgtgaatgg tatgagattt agcgtcgttg gttataatga ttatatggag gatggattcg
    10501 gaaatcttac agaatataaa atatattatt ccagatatta tgatgccaac aaaggtctta
    10561 tcattaagcc cgaaatgaaa ccttttgttg actggaacaa tccagcaaga gtcgatacag
    10621 tgttcattac tacaggcaga ataggcaaca cagaggcttc aattgacaga ggaattgagt
    10681 ttgattttga cttgggtgaa gttaaagtac ttagaacttc attttacttg agtggagctt
    10741 atatacagtc taagtattgg gacaatggaa gaaacttttc taatcctgtg ggtattccgg
    10801 ctggttctgt ttatagccag ggaggatcaa atactcctcc ctttaaactc gaatatcctt
    10861 ccggacgatc aaagaatata aacgaaaggt tttctacttt aataaggaca gttactcata
    10921 ttccatatct caaaatggta gctacactga caggtcaaat agtttggtat tcttctgccg
    10981 aaaccacaaa tcaaaaacaa gatcctattg gttggattga tacagattta tcgtatcatc
    11041 ctattactca acagatgtta gatgatccta gttgcaaaat taaaggcata cttctttctg
    11101 atcaaagaaa agatccaaag gatgatggag caacaagaca gcctttaatt tggattataa
    11161 atgcaagatt atcaaaagat gtatcaaaat cattaggttt ttcatttttt gtaaacaatc
    11221 ttttttacta tacaccttat cagtcaacta acaagtcagg aactttgact gaaaggaata
    11281 cggatacttt ttcatttggt atggaactgt taattaaaat atgattatga aaaaacacaa
    11341 aataatacag atactgaact ctgtgattct ctctattata ttaatatctt gtacaagaga
    11401 agagccttta ccaattcatt cagtacatat tgcagttaat cttcctgcaa attttagagc
    11461 agatgtaaaa tttgccaata aagaagttat tttgacaagt tcaagcaatg aatatcgttt
    11521 tataacaaat gctcaaggtg aggttatagt caacgatctt attccagatg tatataatat
    11581 tattgctcaa tgggaaatgt caggtgttga atataagcag ttaattgcca atcctgagcc
    11641 ggtagaagac agagcaactg tttttttaaa tgtcgctttg aacaattata aaatattttc
    11701 agacgttaat ataacattga atcttgaagc agttatcttg aaaagtcttt taatttcgaa
    11761 gatttattat actggcacaa aggataatgc caataagaat tactcagcag acagctacat
    11821 tgaaatattt aacaattcgg aagacacagt gtttttagat ggtaaatatc tagcactgac
    11881 agaatctatg tctcctgctg cctacccggc aaaagataat cccgatttta tatatgcaag
    11941 gcagatctgt aaatttcctg gagatggaaa tgattattca gttttgccaa gcacaagtat
    12001 tgtcattgct gcaagaagtg ccagagatca tagattaagt gcctcaacat ctgttgattt
    12061 gtctaatgct gattttgaag ttaaagatgc tgatggttct ggtaatccaa ttatcaaagc
    12121 tttgccgata atttctaact caatcccagc tttaaagttc cttaacttgc ttactggagg
    12181 aggaaatgga gtatttattt ttgaaactaa tgaggatatt tctagctggc ctgaagttta
    12241 tgctcctggt aagacttcag gagaaagatt caggaaagta cctgtaggag tggtactaga
    12301 tggagtagag tgtctaaaaa acaatgtggg taccggtcct gatattaatt taaaaagatt
    12361 gccttttgtc attgatgcag gatatacttt cgttaatgca actagcggct atactcatga
    12421 atctttggaa cgtaaagtaa taatcagaaa tggcgtttta aaacttgtgg attcaaataa
    12481 cagtatagag gattttgtga ttgttttaga tcctactcca aaaaaatatg ataaacctga
    12541 actgatgaat aactagacta tgaggaatat atatataata attgtattgt ttttcatgac
    12601 tacagtaaac atcgctgttg gtcaggaaat agattcggtt gcaattgagg cacccgtgaa
    12661 attaaaagaa agagaattac agatttttaa ccaacagtgg aatattactg aaaatgcagc
    12721 tggattgggg ataaataaaa tctcaaacag cagtttgact actattggtt taagcaggag
    12781 tacaggagat tttcataggg cacaggaagg atcttctttt aacaggctgg agtttaatac
    12841 tgatagattt gacaagttta atgatcaatt atatttaaaa ggtgatttta gttttaattt
    12901 ggaaaaagag ttcaacaggg cttggtcaga cgtgtttcac acatacaatt cttctcctta
    12961 tatatttggg agtagtgtag aaggggacta tgaaacacaa agatttagaa tttcttttat
    13021 gatatttact gctcttaatg gcagatttaa ctacggcata ggagttgact ataaagtggc
    13081 agatatgtca cgccaaaaag atcctcgttc aaggtcatat cttttagact ataaaattat
    13141 tccttcaata acatatgcta ttaaccagaa aaatattctg ggcataaatt gttcttacag
    13201 atttgagaaa gagaaaatgc ctaatgttac tacagttcag acagatccaa atttgaaata
    13261 ctatgatttt aaagggctca attatgttga tggcaaaatt ggaggctata aagggtttca
    13321 gagacaattt ataagtgata tcccgggagt agctatccaa tacaactttt tgggggtcag
    13381 ctcaaaatta ttgagttcaa taggtgtcga atatcaagaa caacaaattc ttggaaacaa
    13441 aaaacaaagt ccgggctcat acaactcttt taattacagt ttagtagtag actatgttgt
    13501 aaatagtcaa gattatatac ataacattaa tttaaaagga tcttttagag atggcggtgc
    13561 agatgagttt cgtcaggaac tgaaaagttc taaagatcct attacaggag aaaccacaga
    13621 aacttgggtt acaaattaca aatataaaaa caggtatatg gtcaaagttg ccgatgttta
    13681 tttgtcttac aagcttatga aacaaacgtc agataaaaaa tcaattaaat ggtttattca
    13741 accttatgct gcgtttaata agttttcaaa tacatactat ttaccaaaat cggaatatgt
    13801 ggtcagtaag ttttttgctg gtactaatgc atttttatca atttatagca gaaataacag
    13861 cgatgtagat ttgaacttca atgttgcagg aggatttcct gtcaaaacag atttatcgct
    13921 agttacagtt tctgaattga attcggccat ttatgacatt gacattgagt atcataaaaa
    13981 gaagactata gaaacatatg gtgagattag atatacattt cccatcaaat taggtaagaa
    14041 taagatgatt ggatattcga gattatatgc tggaaacatt tttggtattg gttcttcatc
    14101 ttggttttca acaggtttta gtataggcat aataacatta taaaatgatg aaaaagaaat
    14161 tatgtgggtt attaactctg atagcagggt ttttggttat acaatcttgt gaaaaaatag
    14221 aagataccag atcgtatgca ccggtgcttt tttttgagaa atctaccgag accgttaaaa
    14281 ccgatgtagg aagttatgat gtaaaattag tgttgtcaaa gccggcagaa aaagaaatca
    14341 aggctaaaat aggatttacc ggaactgcca ttgagggaga acactattct gttgcttcta
    14401 atgagatagt atttgctaag ggagataaag aagcgttttt gaaaataaat attctcaatg
    14461 aaaatattta tgatgagtta ataaatatta agtgtttaat ttgtccgagt caggaatatg
    14521 cagttattcc ggaactgaaa tcggagttta tactgaatat gactaaggac ataatactcc
    14581 ctactattag tttcaaatcg actgaacaga atcgtttcac aaatccttat ctggcagaaa
    14641 ctttaactta tgaactgtgt cttacagaag cattaagaat ggacacagag gtaagaatta
    14701 gcgttgaggg agacctctca attggggcag attttttaat caatgaaggc aattcaaata
    14761 ctctaattat accaaaatcg gttttatcaa aaatctttac tgtgactttt aagaaaaaag
    14821 acatggctgg atataataga actctcaagt taactcttgt gccggttact tcgaaaagtt
    14881 gtatagtaaa tgaagaatca tcatttacta acataactgt aaaggaccca gttgtggata
    14941 tgtcttcatt ttttaaaact gctgctcttc tggggggacc tggatatcag atgaatcagg
    15001 ccattaaagc taaagacggt acttattcta gtaacattgt tgttaatgta ttaaacaatt
    15061 cttcaaaagg gaactatatg aaaagcctta ggaacttgtc tttcaacagt gcttttggat
    15121 gttatgctaa cacaccggga ggagatgttt taagattagc tgatttattg aaatttaata
    15181 caacagatac tgtaatcgca gattatggtg ttggaaaaac aacgagattt ttttctccga
    15241 gtgattcttt attaagattt gtcgctgaag gagataatat aagaaaaggg aaagtattcg
    15301 ctcctgcaca gaagtttagc gcgaaacttg ttttaaaaca agattgggag acaggcacta
    15361 atggtaataa gcaatggcat cttgattcta agtcaacggg tggaaatatt tcactttcat
    15421 catatccgct ttcttttgcc acaatggaga tagaattggt aaaactagaa ggcacattca
    15481 atattgatga agatattcca gtaataatct ttgatgcctg gttcagaagt aattcaaaat
    15541 actttatgcg taatattcca gaaagttacg acattgttag ggaaggagat ttataccgaa
    15601 ttagctacaa gtttacacct agataataat gaaggcaaag aaaattagat tattattaat
    15661 gcttttaatt tttcatgttg caattcctgc acagggacat tttataaaga tatgtaatac
    15721 aaatggatat aagtttgaaa ttcctgacta tgttattggt aaagacattt tatttggttc
    15781 gaggatagtt gatattagca agcctgaggc aaaagtttac gcagcaggac aaatgcgcaa
    15841 acctcctgtt ataattcgtt ttacgaagtc aggaaatact ctcagaatgg atcagataat
    15901 taatttttat aagattgaca agaatgatcc aatttatgaa tctgtcgaaa aaaatcaaag
    15961 agtatctgca gtttatactt ttgacattga acaaaaagtt cgaaaggatg gttctttggt
    16021 caatgtgata gacgtttcaa aatttttttt ggatgaagtt actattgctt ggcctttacc
    16081 ggacaatgtg aacaatggaa gattagactc taaactgact tctgttttat tcatgaagga
    16141 gttagatgat cacgtgaata taagagttta ttatggttat acaggcggta aagagccttt
    16201 tagtattact gtacagtact ttatgttgct attaccagaa ctgttaaaac ccagatatga
    16261 tgacgacaga gtcggttatc agacaatatc attaaagaaa tatgaatcag gaaaggtcat
    16321 ttcaaaagaa aaaattatct caagatggag aattgaacca aaattgagcg atgttgaaaa
    16381 gcatagaaaa ggccagcttg ttgaacctga aaacccaata gtgattttta tagatcctgc
    16441 atttccttca gattggattc catatataaa aatgggtatt gaggactggc aaaaagcatt
    16501 tgaaaagata ggctttaaaa atgcaattat tgcaaaggag tatccaaaaa atgatacgga
    16561 ttttgatcct tacgatatta gtgtgaattg tgttagatac attcctgttc aagaggcaaa
    16621 tgcatctgga caaatctgga tagaccctag aagtggggaa attctgcagg gtgatatttt
    16681 atggtggaat gatgttatct cattgataaa aatgtggcgt tttactcaaa cagctgcagt
    16741 agatccggaa gttagaagac ctttttttag caaggagatt gagggagaat tgataagata
    16801 tgctatagct catgaaacgg gtcatatgct tggtctccaa cataatatga gagcgtctta
    16861 tgcatatccg gttgattctc tgaggtcacc gacttttact atgaaatttg gaactactgc
    16921 ctcgattatg gattatgcaa gaaacaatta tgttgctcaa ccaggtgatc ttcagcgggg
    16981 tgttaagatg acaccaccag cacttggacc attcgatttt ttgtctattg agtatggata
    17041 taagattatt tatggtgctg atacagaaaa gaaagaaaga gaagaacttg aaaagatttt
    17101 tatttccaag ggagatgatc caatgtactt ttttgggcct ttaacaatgt cacaggtttt
    17161 tcctgatccc gcctcacagt ctgatgctct gggtgacgat atcataaaat cttcttatta
    17221 tggcattaaa aatacaagaa ttattttgga aaatctatat aagtggacaa ttgatacagg
    17281 aggcgatgaa tcacttctgg tcgaaagata taacgccctt atcaatcagt attatagact
    17341 tttatcttta cctctttcat atgttggagg aaagtaccag ttttatggta atttattagc
    17401 agaaaagcca aaatatattc cagttgaaaa acaaaaacaa attgaagctg tcaattatat
    17461 cactttagaa ttaaaagaag caggcaaaca cacacttaga agagagttat tacccttaat
    17521 cggctccgaa tatgtggata aagtatataa gaaggaagca gatttggtag cattaatggt
    17581 tagtcccatc attcttacaa gattggttca atcgtctttt ttcgattatt cttgcctttc
    17641 tatagaagaa tatttggata tgatagatga taacattctc aatccaagtt atgattataa
    17701 caatcctttc gtaaaaaata ttcaaacctc ttatattcaa gctttgatat ctgtttataa
    17761 acaacaaggc ggagaaatta taaacgggtc aaatgctatt gttttggaat ctatatttga
    17821 tagaatcatt aaaacaagaa gcaagctaac aaacttaata aataagcaat caggaaactc
    17881 tggatataca aaatatcttt taagtcttct gaaaacagat aaatagattt tgatgaaaag
    17941 aatattgatt actttaataa cggttgtatt ttttgttcca tttgcaattg ctgacgaagg
    18001 tttgtggtta ccttttcttt tgaaagatca gaaattttca caaatgcgta aaatgggact
    18061 taaattaagt gcagaagaga tatatagtgt taatcaagct tgtattaagg atgctgttat
    18121 tgggttaatg tcggagggat ctaacctgag aagttatggg acagcaagtt ttatttctga
    18181 taagggactt attattacca actatcatgt tattttgcca tatatccaaa gattttcaac
    18241 acaagaaaat gattttatca aatatggttt ttgggcaaag aattcttcag aagaaattgt
    18301 ttgcagagga ttggaaatga aacaattgat cagaatggaa gatgttaccg aggagatcct
    18361 gaatggaacg aagggcttac aaggtcaaga gaaattaaat aagattaatt cgaatggaaa
    18421 gaaaatagct gaagaagtca cacgaggtac taaatatgag gtttctattc aatctgtttt
    18481 tgggaacaac cagtatatta tgagtctata tatagtttac aaagatattc gaatggttgt
    18541 agttcctcca tttgcacttg ggaaatttgg cggcaataca gataattatt catggccgag
    18601 acatactgct gattttgcta ttttgagagt gtatgcagat caaaataatg aaccgaataa
    18661 ttactcaaag actaatggat tttacaaacc cagaaaaagt cttgtattgt caacatcagg
    18721 ttttaaagaa ggagattttg tgatgatact tggttatcct ggtaaaacaa gacaatatat
    18781 tccttccttc gcattggatg aaattattaa tgttgaaggt cctgctagaa tggatttagc
    18841 caatactaaa attaatattc ttaaacaggc agtggcagaa aatccggaaa tgaagtatag
    18901 atatacagcg aaattatcat cagaaggtaa catctatctg agaacaaagg gacagatgca
    18961 aggagttcgt cgtaccaatc ttattgatag aaagaaagaa gaggagagaa attttattga
    19021 atgggctaac acttctttaa ttaaggacat ggaagagctg tataaagagg ttgcgattta
    19081 taatcttgct gatttatatt ttaaagaagc aggaataaat ggagcagaaa ttgttccttt
    19141 tataggcaaa tttgaaaaac tggtatcaat ttattcaaga aagcattttg ataaaatagt
    19201 ggcagacagg gagtgtaaaa gattaaaggg tcttacagat caattttttg aaagctggag
    19261 ttatgaagtg gataggaaaa tattcagtaa tttgatgtta aaatattatc aaaatcttcc
    19321 cgacaaattc caccctgttt ctatggtgaa atacatgaaa tattatcaaa gtgatgtgga
    19381 agagctttcg aaagatgtat ttcgcaggtc gatttttaca gataaagata ggcttatggc
    19441 ttttcttgat gatgacagaa aaaatgtgga tagtttggta aaagctgatc cgttatatca
    19501 attgtctata ggatattata tggctaatgt tgagaaaatt gcaaatcaaa gggcacagtt
    19561 gcaagcaaaa caattagaga tgtttgaaaa atacatggag ggttttattg aaatgaacga
    19621 caacaaaatc cttttcccag atgcaaacaa ctctcagcgg ttgtcctacg gaaaggttag
    19681 aggaagtaca gcagaagatg ggcttattta tacatcttat acaaccttag atgggatgca
    19741 tgaaaaatat ttaaataaca tttctgaaga agaattttat ctacccgtta aaatccagaa
    19801 actttataaa gaaaaagatt ttggaaaata cggtgacaaa aaggggaaca ttacagttaa
    19861 ttttttgact gattgtcata cttcgggagg aagttcagga tctcctgtat taaacgccag
    19921 aggcgaaatg ataggtctta actttgatag aatttggcaa gggcttgttt ctgactataa
    19981 atttgaacct gaacaatcta gatctattgc tgtagatatt agatttattc tctttattat
    20041 cgacaaatat tcaccatctt cctatatttt ggaagagctt aaaataaagt agcaatttca
    20101 tttctacaaa taatcttatg agaagttctt tggttataag tgaataacat gactattcaa
    20161 cagcacagac agaatggcaa tctatgattg gcttcttttt ttgtaccttt gcatatctat
    20221 tattaacatc ttataatcat ggatttttca ttatctaagg aacaacttct ttttttgaag
    20281 atgattaggg aatttgcaga aaaagaagtt aaacctcttg ctgcagaaat tgatgaggag
    20341 gaacgttttc cggttgaaac agtagaaaag atggccaagt tgggcattat gggtattccg
    20401 tttccggttg aatatggggg agccggggga gacaacatgc tttataccat ggccgtagaa
    20461 gaactttcga aattttgcgg aactaccgga gttatcgtat ctgctcacac atctctctgt
    20521 gctgcaccaa tttatgagta tggtacagag gaacaaaaga aaaagtatct tccaaaattg
    20581 tgctctggag aatggcttgg ggcttttggg ctaactgaac caaatgctgg gactgatgct
    20641 tctgctcagc agactacggc tacactggac ggggattatt acatcctcaa tggcaacaag
    20701 atttttatta cgaatgccgg ttatgcaaaa gtatatatag tgatggctat gacagaccgg
    20761 tcacttggga ctcgtggtat ttcggctttt atagtggaga gtgatgttcc cgggttttcg
    20821 gttggtaaga aggaaaaaaa acttggaata agaggatcct caacaagtga actaatattt
    20881 gaaaactgta gagttccaaa agcaaattta cttggaaaat tgggtggtgg tttcggaatt
    20941 gccatgaaga ctcttgatgg aggaagaatt ggaatagcag ctcaagctct tggtatagcc
    21001 cagggagcta tggatgaaac tgttaaatat gtcaaggaga gaaaacaatt tggtaaacca
    21061 ttaagtcaat ttcaaaatac acagtttcag ttagccgatt taaatacgaa aatagaagct
    21121 gcgcgtttgc ttgtaagatg tgcagccttt aaaaaggatc aaaaacaaaa ttattctaca
    21181 gaggctgcta gagccaaact ttttgcatca gaaacggcga tggagatgac cataaaagca
    21241 gtacagtttc atggtggcta tggctatact cgagaatacc ctgttgagag aatgatgagg
    21301 gatgccaaga tcactgaaat ttatgaaggc acatccgaag ttcaaaggat ggttattgct
    21361 tcaagtttgt taaaataaat taattgaaat gaatatagtc gtttgtataa agcaagtacc
    21421 taatactaca gagataaaac tcaatcctga aacaggaaca atgattcgtg aaggtgttcc
    21481 aagtattatg aaccctgatg ataaaagcgg tttggagctt gcgctaaaac ttaaggacaa
    21541 ttttggtgca catgttactg tcattacaat ggggcctgca agtgctgatg atattttacg
    21601 tgaagctttt gctatggggg cagacagagc gattctcctt accgatagaa agtttgcagg
    21661 agctgacact ttagctactt caagagctct tgcaggtgct ctaagaaaga tggatcatga
    21721 tttaattatt acaggccgcc aggcaattga cggagatact gctcaggttg gccctcaaat
    21781 agcagaacat ttggatattc ctcaagttac ctatgtagag gagtttaact atactgctcc
    21841 aaattatttc aaagttaagc ggacaattga agaaggcagt cagatagtag aggttgagac
    21901 gccatgtctt atgacagtag tctcgtcagc tgccaacaag cctcgttata tgacagttgg
    21961 tggcataatc aatgcttata ggcgtcacgt cgacatttgg ggctgtgcag atattaacgt
    22021 acctgaagaa ttgctcggac tcaaagggtc tcctactaga gtatggaaat catttactaa
    22081 gggtgccaga ggtgccggta agatctatga agttgaacct gccaaagctg ttgagattat
    22141 tattgaaaaa ttgaaagaga aatttattat ttaagagaaa agtcatgata gataaatcac
    22201 aatacaaaga tatttatgtt tttattgaac agcgtgacgg cattgttcaa aatgtctctt
    22261 ttgaattact tacaaaagca gctgaacttg cttctgtctt aaaggagaag gtatatgcaa
    22321 tgttattggg acataatatt gcaactaaat ctcaagatct gattgcttat ggtgcagata
    22381 atgttttgtg tgtagatgct cctgaattgg aagaatattc aactgagcct tatgctcagg
    22441 ctgttgccca gattataaat gaaaggcacc cttctattgt tcttattgga gcaaccacaa
    22501 ttggccggga tcttggtcca cgactttcag ccagaattgg tacaggactt acagctgatt
    22561 gtacaggact cgatatttct gaagacggaa atttgcttat gactcgtcct gcatttggcg
    22621 gaaatcttat ggcaaccatt ctttgcaagg atcaccgtcc tcagatgtct acggtgaggc
    22681 cgggagttat gttgaaaggt gcaagggatg ataatcgcaa gggaattgag gaacggataa
    22741 atattaattt tgacaaatca aaatttcggg taaggattat tgagacggtt aaagaagaga
    22801 agaatcggat tgatattacc gaggctaaag ttttagtttc tggaggcagg ggagttggtg
    22861 atgctgaggg gtttgagcga ttggccaaac tcgcagaagt gattggggca gaagtttctt
    22921 cttcaagggc tatggttgat gctggcataa ttccacatga aaggcaggta ggtcaaaccg
    22981 gtaaaactgt tcgtcctgat ctctattttg ctattggtat ttcaggtgct attcagcatt
    23041 tggcaggtat ggaggagtca ggctatatta ttgccataaa caaggacaag aatgctccca
    23101 ttttccaaac tgctgatttg ggaattgttg gtgatgaaag caaaataatt cctctattaa
    23161 cagaaaagct ctctaagaaa tagatgaata tttcccggat ggtatcttag ctttatttta
    23221 taaagaataa ccggatat
//
LOCUS       DAOTQL010000012        22640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2323_length_22640_cov_4.452734, whole genome shotgun sequence.
ACCESSION   DAOTQL010000012 DAOTQL010000000
VERSION     DAOTQL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_22640_cov_4.452734"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="P5293_01530"
     CDS             complement(<1..815)
                     /locus_tag="P5293_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRR48646.1"
                     /translation="MTKRLFLFLSSMLFIINTTLAQVNVSGTVTDASDGTGIPYATVM
                     VKGTSIATMADIKGAYSLNKVPSNAILVFSSIGYQTIEIPVEGRSIVNAVMSQQAVGL
                     DEVMVVAYGSVKKGSYSGSATMIKGDAIKDIPVMTFEQAITGAVPGVSIAQTSGQPGG
                     LPEIRIRGFSSFNAGNDPLYVIDDIPATSGDLSSANIWSSTMNYLNPSDIETITILKD
                     AAAASLYGSRAANGVILITTKKGQVGKIVSTFKASAGVSYFAYNNYPLANDQEA"
     gene            1160..1759
                     /locus_tag="P5293_01535"
     CDS             1160..1759
                     /locus_tag="P5293_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRR48647.1"
                     /translation="MITSDLQQTPTGGGLLSEKEFEALYRQSYRKALYYCNQYIVDIE
                     KSKEIVQEAFINLWEKRQSIHIESGNPEYYLLTSVRNLAYNAIRNKIREYKRNGKAIC
                     IDDKINEKSLSQEPCDLLTFNDINIAINTTIDSMSQKIREVYIMSREMEMTYPQIAEK
                     LGISVKTVEYRLSKALALFRKALKKYLPLILLFFNNFFR"
     gene            1787..2782
                     /locus_tag="P5293_01540"
     CDS             1787..2782
                     /locus_tag="P5293_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HRR48648.1"
                     /translation="MDKIIIKYLSGTANEKEKEQLLQWIEESDKNAKFFADLKHAWTL
                     SKTKNDPLSDKEVVHFMKMIKREKQRHLPSWWIAAAAAAVVILISSLFISMQRKIESY
                     KEDISYMLTQAGPRYEYSTPYGVKGKVYLPDGSAVWLNSGSRISFPGKFNGKSRELDF
                     SGEGYFEVIPDSQKPMNVRLRSGLTIKVVGTEFNLSSYDDDKKVSLLLLSGKVNINNA
                     DGKKIYSVQPNEKITINLIDNTLLRNIPQEIAPTIGWKNGWLIFDEAPLEEVFKKMER
                     WYGQKIIVEDQSVYSKKLTAKFHEESISQVLGLMNQISLINYKIVDSIAYISKFN"
     gene            2947..6330
                     /locus_tag="P5293_01545"
     CDS             2947..6330
                     /locus_tag="P5293_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR48649.1"
                     /translation="MKSILNELEKKSDFTFIYSPSTIDVNQKISITANSEDFFSLLDR
                     LCRIAKISYKTSGRQIVLTPLATSERALNQQNVTENGKKITGTVLDENREPVAGAYVI
                     LQNNKKIGTYTDAKGKFEINIPNEYANDAVLLFSFFGLKATEVKIGDDNNISVVMPFE
                     EKQLDDVVVTGYQTISRERATGSFAIVTQNVLNTKMQTNIINKIEGMVAGLNIYRGSV
                     QIRGVSTINGNKNPLYVVDGVPFEGEPGSNASPLDVINPADVVNVTVLKDATAASIYG
                     ARSANGVIVITTRNGEEGATRVNYNGSVSMIGLPDRGYANKMNSSELVDYQLMLMETY
                     PKLTRKGIREYQNPVQSLMLDKKEGKITQEQLEQALIPYRNFDRYDQVKNEFLRKYSI
                     KHQHNLALTGGTKIHKYNFSVNYSGTAPYEKAQYEDRLGFNLKNTFDFFKWLSVDAGI
                     LYSQVKSDYDNGILGMSYLDAGGASYLRFREDNGEPSQWYQTKSQYEIDRLKALGLQD
                     ETFYPVNELKNRRYTAKTNYLNINLNARIKILDGLTASIRYQTEQSNGFTKQYDTKDA
                     ISVKTMINNAVQMVNGVPKYNIPVGGQIVQKWMDNYSYTFRAQIDYNKQLNDNNIVQF
                     LAGAEARKVVTSGNGFYRLGYDDNNLGYSEIDALTLSKMLSGTESIYGTFSFSNKTPA
                     STFVDNRYLSFYANGSYTLNNKATFTGSIRIDQSNLFGTDPKYQYRPLWSLGAHYELL
                     RNYNWIDRLAVRVTYGINGNIPKLNGPYLIAKVDRNNYYTNENAMYIASPPNPQLRWE
                     KTKVFNVGVDFNLLKNRLDGSIEFYDKHTNDLLGPFAIDPTLGWTDVDINFGSMYNRG
                     VEIALNSQNIKKEHFSWSSSLLFSYNKNMITRVEETDESAYSYYYGTNIRKGYPMGAL
                     FSVRYKGLNQNGAPVGLTKDGQEILNYNQLTKDDLVYSGVVTPPYNASLTNSISWKGF
                     ELSFMFIYSGGHVMRDVAASYTITSHPMYAVSNVDKNMANYWKKPGDENKPNVNPAFM
                     FQSTVRNGEYIYKAADKFVKKADFIKLRDLILSYNLPSKITKKMGIGAATINFQARNL
                     WWWAANGNLDPEVWTGTSLSPTRGTKYPAEFIVGLNINL"
     gene            6345..7787
                     /locus_tag="P5293_01550"
     CDS             6345..7787
                     /locus_tag="P5293_01550"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR48650.1"
                     /translation="MNKKILINILAVSLLISVTGCDDYLNNVPKGMTIPQYMEDYQKL
                     LNSQSLINASDGMLDYLTDNIHLLDKNATASYYIYINKDESVRNLYSFKPGQVYVEGT
                     KDYIWNNAYERLFTLNTVINEVMDSKGGSEEAKLRIKAEALFGRAYEYYNLVNTYAKQ
                     YNSQTASKDLGIPYLTEADINQKYQRHTVAEVYEKMLADLDEAASHIPDITANRAHPN
                     KAALYSFYAKIYLTMGDYTKALKYANDALALNSALLNLNNYDKAEGTTWGRVYKKGNP
                     SERLPDINHPEANYVKWLSGSLQGSVMLSSEMRDLYKKDLNGATDLRKEYFFAENQVN
                     LGGSTDYFPGECAFVLYANFNVGFTSTENYFIAAECEARIGSKDKAMQLINTVRNNRM
                     MNNEQLTASTKEEALIKVLEEKRREFCFKGPMRLFDLKRLNLESQFQKTITHTADGET
                     FILPPNDVRYIMPVNKEILNFNPDFPVYER"
     gene            7816..8979
                     /locus_tag="P5293_01555"
     CDS             7816..8979
                     /locus_tag="P5293_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRR48651.1"
                     /translation="MRKVYCQIFIFAAMCLYFSPLSAQKAIRFEKGNWNEIVEKAQKA
                     KKMIFVDFYTQWCGPCLNMSEEVFSLPAVYNFYNTNFINAKIDAEHGEGKELAAKYQV
                     KVYPTYLFIDPTTGNALHKSSGRQEPDTFIFTGESALNPRLRSFYLEANYDKNKNDLS
                     FLNDYAKYKSSIYDREGVAKVLKQLEKLDVKLDNSLVWELFVNNITGYDNSFICEVIT
                     NYDRYVKLYGKEDVDNKLAKETSYAPVDYLKGLPDFKGKNTNIVINEINTAQRDANYD
                     EAIRLIDIALKDTTIDRNKFLNALRFTVRLNDGKKYPDLWVKKCGEYIRYIAYNYSDR
                     KDPYIHYEYALYLESLLRQIPSANLYLPESILEKPKSGKSEYTMRPDNLKPHP"
     gene            9265..9864
                     /locus_tag="P5293_01560"
     CDS             9265..9864
                     /locus_tag="P5293_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRR48652.1"
                     /translation="MITSDLQQTPTGGGLLSEKEFEALYRQSYRKALYYCNQYIVDIE
                     KSKEIVQEAFINLWEKRQSIHIESGNPEYYLLTSVRNLAYNAIRNKIREYKRNGKAIC
                     IDDKINEKSLSQEPCDLLTFNDINIAINTTIDSMSQKIREVYIMSREMEMTYPQIAEK
                     LGISVKTVEYRLSKALALFRKALKKYLPLILLFFNNFFR"
     gene            9892..10887
                     /locus_tag="P5293_01565"
     CDS             9892..10887
                     /locus_tag="P5293_01565"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HRR48653.1"
                     /translation="MDKIIIKYLSGTANEKEKEQLLQWIEESDKNAKFFADLKHAWTL
                     SKTKNDPLSDKEVVHFMKMIKREKQRHLPSWWIAAAAAAVVILISSLFISMQRKIESY
                     KEDISYMLTQAGPRYEYSTPYGVKGKVYLPDGSAVWLNSGSRISFPGKFNGKSRELDF
                     SGEGYFEVIPDSQKPMNVRLRSGLTIKVVGTEFNLSSYDDDKKVSLLLLSGKVNINNA
                     DGKKIYSVQPNEKITINLIDNTLLRNIPQEIAPTIGWKNGWLIFDEAPLEEVFKKMER
                     WYGQKIIVEDQSVYSKKLTAKFHEESISQVLGLMNQISLINYKIVDSIAYISKFN"
     gene            11052..14435
                     /locus_tag="P5293_01570"
     CDS             11052..14435
                     /locus_tag="P5293_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR48654.1"
                     /translation="MKSILNELEKKSDFTFIYSPSTIDVNQKISITANSEDFFSLLDR
                     LCRIAKISYKTSGRQIVLTPLATSERALNQQNVTENGKKITGTVLDENREPVAGAYVI
                     LQNNKKIGTYTDAKGKFEINIPNEYANDAVLLFSFFGLKATEVKIGDDNNISVVMPFE
                     EKQLDDVVVTGYQTISRERATGSFAIVTQNVLNTKMQTNIINKIEGMVAGLNIYRGSV
                     QIRGVSTINGNKNPLYVVDGVPFEGEPGSNASPLDVINPADVVNVTVLKDATAASIYG
                     ARSANGVIVITTRNGEEGATRVNYNGSVSMIGLPDRGYANKMNSSELVDYQLMLMETY
                     PKLTRKGIREYQNPVQSLMLDKKEGKITQEQLEQALIPYRNFDRYDQVKNEFLRKYSI
                     KHQHNLALTGGTKIHKYNFSVNYSGTAPYEKAQYEDRLGFNLKNTFDFFKWLSVDAGI
                     LYSQVKSDYDNGILGMSYLDAGGASYLRFREDNGEPSQWYQTKSQYEIDRLKALGLQD
                     ETFYPVNELKNRRYTAKTNYLNINLNARIKILDGLTASIRYQTEQSNGFTKQYDTKDA
                     ISVKTMINNAVQMVNGVPKYNIPVGGQIVQKWMDNYSYTFRAQIDYNKQLNDNNIVQF
                     LAGAEARKVVTSGNGFYRLGYDDNNLGYSEIDALTLSKMLSGTESIYGTFSFSNKTPA
                     STFVDNRYLSFYANGSYTLNNKATFTGSIRIDQSNLFGTDPKYQYRPLWSLGAHYELL
                     RNYNWIDRLAVRVTYGINGNIPKLNGPYLIAKVDRNNYYTNENAMYIASPPNPQLRWE
                     KTKVFNVGVDFNLLKNRLDGSIEFYDKHTNDLLGPFAIDPTLGWTDVDINFGSMYNRG
                     VEIALNSQNIKKEHFSWSSSLLFSYNKNMITRVEETDESAYSYYYGTNIRKGYPMGAL
                     FSVRYKGLNQNGAPVGLTKDGQEILNYNQLTKDDLVYSGVVTPPYNASLTNSISWKGF
                     ELSFMFIYSGGHVMRDVAASYTITSHPMYAVSNVDKNMANYWKKPGDENKPNVNPAFM
                     FQSTVRNGEYIYKAADKFVKKADFIKLRDLILSYNLPSKITKKMGIGAATINFQARNL
                     WWWAANGNLDPEVWTGTSLSPTRGTKYPAEFIVGLNINL"
     gene            14450..15892
                     /locus_tag="P5293_01575"
     CDS             14450..15892
                     /locus_tag="P5293_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR48655.1"
                     /translation="MNKKILINILAVSLLISVTGCDDYLNNVPKGMTIPQYMEDYQKL
                     LNSQSLINASDGMLDYLTDNIHLLDKNATASYYIYINKDESVRNLYSFKPGQVYVEGT
                     KDYIWNNAYERLFTLNTVINEVMDSKGGSEEAKLRIKAEALFGRAYEYYNLVNTYAKQ
                     YNSQTASKDLGIPYLTEADINQKYQRHTVAEVYEKMLADLDEAASHIPDITANRAHPN
                     KAALYSFYAKIYLTMGDYTKALKYANDALALNSALLNLNNYDKAEGTTWGRVYKKGNP
                     SERLPDINHPEANYVKWLSGSLQGSVMLSSEMRDLYKKDLNGATDLRKEYFFAENQVN
                     LGGSTDYFPGECAFVLYANFNVGFTSTENYFIAAECEARIGSKDKAMQLINTVRNNRM
                     MNNEQLTASTKEEALIKVLEEKRREFCFKGPMRLFDLKRLNLESQFQKTITHTADGET
                     FILPPNDVRYIMPVNKEILNFNPDFPVYER"
     gene            15921..17099
                     /locus_tag="P5293_01580"
     CDS             15921..17099
                     /locus_tag="P5293_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRR48656.1"
                     /translation="MRKVYCQIFIFAAMCLYFSPLSAQKAIRFEKGNWNEIVEKAQKA
                     KKMIFVDFYTQWCGPCLNMSEEVFSLPAVYNFYNTNFINAKIDAEHGEGKELAAKYQV
                     KVYPTYLFIDPTTGNALHKSSGRQEPDTFIFTGESALNPRLRSFYLEANYDKNKNDLS
                     FLNDYAKYKSSIYDREGVAKVLKQLEKLDVKLDNSLVWELFVNNITGYDNSFICEVIT
                     NYDRYVKLYGKEDVDNKLAKETSYAPVDYLKGLPDFKGKNTNIVINEINTAQRDANYD
                     EAIRLIDIALKDTTIDRNKFLNALRFTVRLNDGKKYPDLWVKKCGEYIRYIAYNYSDR
                     KDPYIHYEYALYLESLLRQIPSANLYLPESILEKPKSGKSEYTMRPDNLKPKPHLSDK
                     "
     gene            complement(17068..18390)
                     /gene="argH"
                     /locus_tag="P5293_01585"
     CDS             complement(17068..18390)
                     /gene="argH"
                     /locus_tag="P5293_01585"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRR48657.1"
                     /translation="MAKLWDKGVLSEDRLVDRFTVGSDREFDERLAEYDVIGSIAHIT
                     MLNSVGLLEDEEFNILKSELEYLLDTINKGEFKIEPDVEDIHSQVELYLTKKVGETGK
                     KIHSGRSRNDQVLVDIKLYLKHEILQFKNEIGDLFKLLQSLSEKHKDVLMPGYTHYQV
                     AMPSSFGLWFGSYAEALVDDVYLLVAAWKVVDQNPLGSAAGYGSSFPIDRDMTTKLLG
                     FSTMNYNSISAQLSRGKSEKAVANALASIASTLNKLAVDCTMYMNSNYGFISFPDELT
                     TGSSIMPHKKNPDVWELIRANCNRIQSVPNEVSMLATNLPHGYHRDFQLLKEILFPAL
                     DRMHNCIKMSMLMLSSIKINREILEDSKYDYLFTVEEVNRLVLEGVPFREAYKRVGEE
                     VASGRFVPEKKIAHTHKGSIGNLCTFEIKSKMDEALAQFTCPTGEVLV"
     gene            complement(18395..19462)
                     /locus_tag="P5293_01590"
     CDS             complement(18395..19462)
                     /locus_tag="P5293_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR48658.1"
                     /translation="MVDNKYFSEAISLLREVVSTPSVSGEENEVSNLLCNYLSSKGYT
                     PERISNNIIVNSRRYDTAKKILLLNSHMDTVRAVGHYSFDPYDPQLDPQRIFGLGSND
                     AGASLVSMIQTFLFFEQRDEPLPFNLMLLISAEEETSGKRGIKLALRHIMRPDCAIVG
                     EPTSMNMAIGERGLLVVDALARGICGHAARNEGLNAIMIALDDINWLKSYKFPLISEK
                     MGEVRCTVTQIEAGTQHNVIPDSCKFVVDIRPTDCYTNSDIMDILRAHMKSELTARSL
                     TNKSSLTPCDHPLIAAAEACGIKTYISPTTSDWMRLDFPAVKMGPGDSSRSHRADEFV
                     WTREIEDGIKGYIKLISKLKL"
     gene            complement(19473..20249)
                     /gene="argB"
                     /locus_tag="P5293_01595"
     CDS             complement(19473..20249)
                     /gene="argB"
                     /locus_tag="P5293_01595"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRR48659.1"
                     /translation="MKQLTVVKIGGNIVDRSAALEEFLKQLGKESGPRVLVHGGGAMA
                     TELSSKIGLKVNMQDGRRVTDIDTLKLVTMVYAGWINKSIVALLQKFGTNAIGLSGAD
                     GDIVPATRRSPYPIDFGFVGDVNPEDINLPLLDSLLGRGITPVFCAITHDRNGSLLNT
                     NADTMASSLAIALSRDYSTKLVYCFEKEGVLSNPEDDSSVIPLITRGSFESLKESKVV
                     SGGMLPKLETAFAAIENGVSEVYIKHALNLNNNKGTILRP"
     gene            complement(20263..21219)
                     /locus_tag="P5293_01600"
     CDS             complement(20263..21219)
                     /locus_tag="P5293_01600"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HRR48660.1"
                     /translation="MNKFTCVKDIEPLNIALAKATEVKANPFADQALGKNKTLLMIFF
                     NSSLRTRLSTQKAAMNLGMNVIVLDINQGAWKLETERGVVMDGDKSEHILEAIPVMGS
                     YCDIMGVRSFAKLEDKSYDYSEIVLNQFIEYSGRPVFSMESATRHPLQSFADLITIEE
                     YKKVERPKVVLSWAPHPKPLPQAVANSFAEWMNATDYEFVITHPEGYELSEEFTKGAK
                     IEYDVEAAYKNADFIYAKNWSKFRDPDYGKVVNKDRNWTVDARKMKLTNNAKFMHCLP
                     VRRNMIVTDEVIESPDSIVIPEAANRVVSAQVVLREILKSIT"
     gene            complement(21222..22364)
                     /locus_tag="P5293_01605"
     CDS             complement(21222..22364)
                     /locus_tag="P5293_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRR48661.1"
                     /translation="MKPFNVYKTWPVEPVKAKGCKVWDRDGIEYLDLYGGHAVISIGH
                     AHPYYVKMLCEQMEKISFYSNSVQNSLQDELADKLGKISGYDDYSLFLCNSGAEANEN
                     ALKLASFASGKKRVVSFKEAFHGRTSGAVAVTDNPNYVSPFNAGHEVTFVPINSVSAV
                     ENELSKGDVAAVIIEGIQGVAGIYEPDASFLKELSLLTRKFGAFLILDEVQSGYGRTG
                     QFFAHQHSGIKPDIITTAKGMGNGFPIGGVIISSEIKPVPGMLGSTFGGNHLACTAAI
                     AVLDVLQSESLIQNAAIVGEWLIASLKKIQADIPGKITSVRGRGLMIGIELDSKHTGV
                     RDRLLFENHIFTGSSKSNIIRILPPLTISMEEAEYFLKSITYQLNR"
     gene            complement(22367..>22640)
                     /locus_tag="P5293_01610"
     CDS             complement(22367..>22640)
                     /locus_tag="P5293_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRR48662.1"
                     /translation="RFDLSEMIEHFNSVYKNSPYVIISDENISLKEVVNTNKALIHLE
                     KHNGYWHLTSVIDNLIKGASGQAIQNMNIMFGYEENCALSLKGSAF"
BASE COUNT         7519 a         4345 c         4231 g         6545 t
ORIGIN      
        1 gcttcctggt catttgcaag tggataattg ttataagcaa aatatgacac tccggcactt
       61 gctttgaatg tagagacaat cttaccaacc tgtccttttt tggtagttat taagattaca
      121 ccatttgcag ctctcgaacc atacaaagat gcagccgcag catccttgag aattgtgata
      181 gtttcaatat cactcggatt caggtaattc attgtacttg accaaatatt tgcagatgaa
      241 aggtctccac ttgtagcggg aatatcatct attacatata gaggatcatt accggcatta
      301 aaggagctga atccacgaat tcttatctcg ggaagacctc cgggttgacc tgaagtttga
      361 gctattgaca cacctggtac tgctcctgta atagcttgtt caaaagtcat taccggaatg
      421 tcttttatgg catctccctt gatcattgtg gcagatcctg aataagaacc ctttttgacg
      481 ctgccataag caactaccat tacctcatca agaccaactg cctgttgaga cataactgca
      541 tttacaatag acctgccttc gacaggaatt tcaattgttt gatagccaat tgaagaaaaa
      601 acaagaattg cattagaagg cactttgtta agcgaataag caccttttat gtctgccatt
      661 gttgcaatag atgtaccttt taccataaca gtggcatatg gaattcctgt accatctgac
      721 gcgtcagtga ctgtaccact gacatttact tgcgccaatg tagtgtttat aataaacaac
      781 attgacgaca aaaagagaaa taatcgtttt gtcatagatt tggcgtttag gttgggttaa
      841 aaaattatct gttaaattta ataaaaaaaa caatcacctg tcggagtttg ttgtaaatgg
      901 ctcattaata agtatttaat ttttgatatt tttaaaagac tattttttaa gatactgatt
      961 acttgtcatt tacaacaaac tccgacaggt gcaggtggag gccgagggct gggatgctcg
     1021 gccagtgggg ctgtcgacta aattattatt atttttgcat cataaacctg aaccagtaac
     1081 acctgtcgga gtttgttgta aactcttaat tttcagacat ataaatttac aatttaaaac
     1141 caaaataaaa aataaataat tgattacaag cgatttacaa caaactccga caggtggagg
     1201 cttgctctcc gaaaaagagt ttgaagctct ttaccggcag agctaccgta aagctttgta
     1261 ctattgtaat caatacattg tagacatcga aaagtctaaa gagattgtgc aagaagcttt
     1321 catcaacctt tgggaaaaac gtcaaagtat tcatattgaa tccggcaatc cggaatatta
     1381 tcttcttact tccgtcagga atctggcata caatgctata agaaacaaga taagagagta
     1441 taaaagaaac ggaaaagcta tttgcattga tgataaaata aacgaaaagt cactctcaca
     1501 agagccgtgt gacttactta cattcaatga tataaacatt gcaattaata caactattga
     1561 ttccatgtct cagaaaatcc gggaggttta cataatgagc agagaaatgg agatgaccta
     1621 ccctcaaatt gccgaaaagc tgggcatatc tgtcaaaacg gtagagtaca gactcagtaa
     1681 ggcacttgct ttatttagaa aagctttgaa aaaatactta cccctgattc ttttattttt
     1741 taataacttt ttcaggtgaa attaataatt tggtgtaatt aatattatgg ataaaattat
     1801 aataaaatat ctatctggca ctgcaaacga gaaagaaaaa gagcaattgt tgcaatggat
     1861 tgaagagagt gataagaatg caaaattctt tgccgaccta aagcatgcat ggactctttc
     1921 caaaactaaa aatgatcctc tttccgacaa agaagtggtt cattttatga aaatgataaa
     1981 gagagaaaaa cagagacacc tgccttcatg gtggattgca gcagctgctg ctgcagttgt
     2041 gattctaatc tcttcgctgt tcataagtat gcaaagaaaa atagaatcat acaaggaaga
     2101 tatttcttac atgcttaccc aagcaggacc tcgatatgag tattcaacac catacggagt
     2161 aaaaggcaag gtttaccttc ctgacggaag tgctgtctgg cttaattctg gcagtaggat
     2221 ttcattccca ggaaaattca acggcaagtc aagagagttg gatttttcgg gtgaaggtta
     2281 ttttgaggtc attcctgact cgcaaaaacc aatgaatgtc aggctccgct cagggctaac
     2341 aataaaagtt gttggcactg aattcaacct atcctcctac gatgacgaca agaaagtatc
     2401 cttacttctt ctcagcggca aagtgaatat taacaatgct gatggtaaaa aaatctattc
     2461 ggtacagccc aacgaaaaaa tcacaataaa ccttatcgat aatacattat tacgcaatat
     2521 cccacaggaa attgctccaa cgataggctg gaaaaacggc tggcttattt ttgacgaggc
     2581 tcctctcgaa gaggtgttta aaaaaatgga gcgttggtat ggacaaaaaa ttattgtaga
     2641 agaccaaagt gtttattcaa agaaacttac tgctaaattt catgaagagt caatatctca
     2701 agttcttgga ctgatgaatc aaatctcact tatcaactac aaaattgttg acagtatagc
     2761 ctatatatct aaattcaatt aattaatatt atttaggggg tatacagatt ttggtgtatc
     2821 tactgtcaaa taaacttaaa caaaatttgt atgcgaaaat tatgtctgtt tattctgact
     2881 ttattggttt cagccacatt attttcccaa aatgtcggaa ttaccttcaa aggagaaaat
     2941 ctccctttga aaagtatctt aaatgaactt gaaaaaaagt ccgattttac atttatctat
     3001 tctccctcga caatagatgt aaaccaaaag atctccatta cggccaattc tgaagatttt
     3061 ttttccctcc tcgacagatt gtgccgaatt gctaaaattt cctataagac aagtggaaga
     3121 caaattgttc taacaccttt ggcaacttct gaaagggcac taaaccagca gaatgtaaca
     3181 gaaaacggaa aaaaaatcac aggtacagtt ctggatgaaa accgagaacc tgtagcgggt
     3241 gcatatgtaa tacttcaaaa caacaaaaaa attggcactt atacagatgc aaagggaaag
     3301 tttgaaatca acatccccaa tgagtatgca aatgatgctg tcttgctttt ttcttttttc
     3361 ggattaaaag ctactgaagt taaaattggt gatgataaca atatttctgt cgtaatgcca
     3421 tttgaagaaa aacagttgga tgatgtagta gttaccggct atcagacaat ttcgagagag
     3481 agggcaaccg gttcatttgc catagtaact caaaatgttc tcaacaccaa aatgcagaca
     3541 aatattatta ataaaattga gggaatggtt gccggtctta atatttatcg tggatctgtg
     3601 caaataagag gtgtttctac tattaacgga aataaaaacc cactttatgt tgtagatggc
     3661 gtcccttttg aaggcgaacc aggaagtaat gcatctcctc tcgacgttat taatcctgca
     3721 gatgtggtga acgttactgt actcaaggat gcaacagcag cctcaattta cggagcaaga
     3781 tctgcaaatg gtgtaatagt cataactacc cgaaatggcg aagagggagc aacaagggta
     3841 aattacaatg gatcagtaag catgataggt cttcctgaca gaggatatgc caataaaatg
     3901 aacagcagtg aacttgtaga ctatcagtta atgctaatgg agacttatcc aaaacttacc
     3961 cgtaagggaa taagagaata tcagaatcca gtacagtctc tcatgctgga caaaaaagag
     4021 ggcaaaataa ctcaggaaca gttggaacag gcattaattc catacagaaa ttttgacagg
     4081 tatgatcagg ttaaaaacga gtttctaaga aaatactcaa ttaaacatca gcacaatctt
     4141 gcacttacag gcgggactaa aattcacaag tacaactttt ccgtaaacta ttccggtact
     4201 gctccttatg aaaaggctca gtatgaagac cgacttggat ttaatctgaa aaacaccttt
     4261 gatttcttta aatggctaag tgttgacgca ggcattcttt acagtcaggt aaagtcagat
     4321 tatgacaacg gaatattggg aatgagctac ctcgatgcag gtggagcttc atacctcaga
     4381 ttcagggaag ataatggaga gccatctcaa tggtatcaaa ccaaatctca atatgagata
     4441 gaccgattga aagctttagg tcttcaagat gaaacattct atcctgtaaa cgagctgaag
     4501 aacagaagat acactgcaaa aacaaattat cttaacatca atttaaatgc ccgcattaaa
     4561 atacttgatg gcttgacagc aagtatcaga tatcagaccg aacagagcaa tggttttacc
     4621 aaacaatatg acaccaaaga tgcaataagt gttaaaacca tgattaacaa cgccgttcag
     4681 atggttaacg gagtaccgaa atataatatt ccagtcggtg gacaaatcgt acaaaaatgg
     4741 atggataact attcttatac tttcagagct caaattgatt acaacaaaca attgaacgat
     4801 aataatattg ttcagtttct ggcaggtgct gaagctagaa aagttgttac ctccggaaat
     4861 ggcttttatc gtcttggata tgatgacaat aatcttggat acagtgagat agatgccctg
     4921 acactctcca agatgctttc aggaacagag tcgatttatg gtacattctc atttagcaac
     4981 aagactcctg caagtacatt tgtagataac agatatcttt ctttctatgc aaacgggtca
     5041 tatactttga acaacaaagc tacttttact ggaagtatca gaattgacca atctaatctt
     5101 tttggtactg atccgaaata tcaatatcgt cccctttggt ctttaggtgc tcattacgag
     5161 ctcttaagaa attacaattg gatagataga ctggccgtca gagttacata cggaattaat
     5221 ggcaacattc ctaaactcaa tggcccgtac ctgatagcta aagttgacag gaataactac
     5281 tataccaacg aaaacgcaat gtacattgct tctcctccca atcctcagct aagatgggaa
     5341 aagacaaagg tttttaacgt tggagtagat tttaacttgc tgaaaaacag acttgatggc
     5401 tcaattgaat tttacgacaa gcacaccaat gatctattgg ggccatttgc catagaccct
     5461 actttgggat ggacagatgt cgatatcaat tttggcagca tgtacaatag aggagtggaa
     5521 atagccctga acagtcaaaa tatcaaaaaa gagcatttta gctggagctc ctccttgttg
     5581 tttagttaca acaaaaatat gataacaagg gttgaagaaa ccgacgagtc tgcctacagt
     5641 tattattacg gcactaatat tcgtaaagga tatccaatgg gtgcactgtt cagtgtaaga
     5701 tataaaggcc ttaaccaaaa tggggctcct gtaggactta caaaagacgg gcaagagatt
     5761 ttgaattaca atcagctgac aaaggatgat ttggtatact ccggcgttgt cactcctcca
     5821 tataatgcat ctttgacaaa ttcaatttca tggaagggat ttgaactttc attcatgttt
     5881 atctattcag gcgggcatgt aatgagagac gttgctgcat cgtacacaat tacaagccat
     5941 ccaatgtatg cagtcagcaa cgtggacaaa aatatggcaa attactggaa aaaacccggt
     6001 gatgaaaata agccaaatgt caatcctgca ttcatgtttc agtcaacagt aagaaatggc
     6061 gaatatattt acaaggctgc agacaaattt gttaagaaag cggactttat caagttgcgt
     6121 gatcttatac tctcctataa tctgccatcg aaaattacta aaaagatggg cataggagca
     6181 gcaactatta atttccaggc aagaaatctt tggtggtggg ctgccaatgg aaacctcgat
     6241 cctgaagttt ggacaggcac atcactctct cctaccagag gcactaaata tcctgccgaa
     6301 tttattgtag gattaaatat caacctataa aaatattcaa caccatgaat aagaaaattt
     6361 taataaatat attggctgta tcacttctga tatctgtaac aggctgtgac gactatttga
     6421 acaatgtgcc caaaggcatg actattcctc aatatatgga agattatcag aagctgttaa
     6481 actctcagtc cctgattaat gcttccgacg gaatgctcga ttaccttact gataacatcc
     6541 atctgcttga taaaaatgca actgcttcat attatatata cataaataag gatgaatccg
     6601 taagaaatct ttattccttt aaaccaggac aggtctatgt tgaaggcaca aaggattata
     6661 tatggaataa tgcctacgaa agacttttta ctctgaatac tgtcattaat gaagtcatgg
     6721 attctaaggg aggaagtgaa gaggcaaaat taagaataaa ggctgaggct ctttttggaa
     6781 gggcttatga atactacaac ctggtaaaca cctacgctaa acagtataac agtcaaactg
     6841 ccagcaaaga tttaggtatt ccttacctca ctgaagctga cattaaccaa aagtatcaaa
     6901 gacatactgt tgccgaagtt tacgaaaaaa tgcttgcaga cttggatgaa gctgcttctc
     6961 acatacccga tattacagct aacagggctc atcccaataa ggctgcgttg tattcttttt
     7021 atgcaaaaat ttatctcaca atgggagact acactaaggc tctcaaatat gctaatgacg
     7081 cactcgctct caacagtgca ctgctcaact tgaataatta cgacaaagca gaaggtacaa
     7141 catggggaag agtctacaaa aaaggaaatc cttctgaacg acttcctgac attaaccatc
     7201 ctgaagcaaa ttatgtgaag tggctgtctg gttctcttca aggtagtgtg atgcttagta
     7261 gtgaaatgag agatctttac aaaaaggatt taaatggcgc aactgacttg agaaaggaat
     7321 attttttcgc cgaaaatcag gttaatcttg gtggaagtac tgactatttc ccgggagagt
     7381 gcgcatttgt actctacgca aacttcaatg taggatttac ttctacagaa aattatttca
     7441 tagcagctga gtgcgaagcg agaattgggt cgaaagacaa ggccatgcaa cttatcaata
     7501 ctgtcagaaa caacagaatg atgaataatg aacagcttac tgcatcgacc aaagaagaag
     7561 ctttaatcaa ggtacttgaa gagaagcgga gagagttttg tttcaaaggt ccaatgaggt
     7621 tgtttgatct taaacggctt aatctcgaat cacaattcca aaaaacgata actcatactg
     7681 ccgatggaga aacatttatc ctgcctccga acgatgtaag atacataatg cctgttaata
     7741 aggagattct taatttcaac cctgattttc cggtatacga aagataaaaa caaagcgaaa
     7801 tatgattata aaacaatgag aaaagtttat tgtcagatct ttatatttgc agcaatgtgc
     7861 ctctattttt caccactctc tgcccaaaag gctattcgct tcgaaaaagg caactggaat
     7921 gagattgtgg aaaaggcaca aaaagcgaaa aaaatgattt ttgtcgactt ttacactcaa
     7981 tggtgtggtc catgtttaaa tatgagtgaa gaggttttca gtttgccggc tgtttacaat
     8041 ttttacaaca ctaatttcat taatgccaaa attgatgctg agcacggtga aggaaaagaa
     8101 cttgcggcaa aataccaggt caaggtttat cctacatatc tttttataga tccaacaaca
     8161 ggaaatgctc ttcataaaag cagtggccga caggaacctg atactttcat ctttaccggt
     8221 gaaagtgctc ttaatccaag actcaggtct ttctatctcg aggcaaatta tgataaaaat
     8281 aagaatgatc tatcattttt aaatgattat gccaaataca agagttcaat atacgaccgt
     8341 gagggagtag ccaaagtcct taaacaattg gaaaaacttg acgtcaaact ggacaattca
     8401 ctggtctggg aactgtttgt aaataatatt acaggctatg acaactcctt tatttgtgaa
     8461 gttatcacaa attatgacag atatgttaaa ctatacggca aggaagatgt cgacaacaaa
     8521 ttggccaaag agacatcata tgctcctgtt gattatctta aagggttacc cgattttaaa
     8581 ggaaagaaca caaatattgt aatcaacgaa attaacacgg cacaaagaga tgccaattac
     8641 gatgaagcaa taagattgat tgacatagct cttaaagata caacaattga tcgcaataaa
     8701 tttcttaatg ctcttagatt tactgttaga ttaaatgacg ggaagaaata tcctgacttg
     8761 tgggtaaaaa agtgtgggga gtatatcagg tacatcgctt acaattactc tgacaggaaa
     8821 gatccttata ttcattatga atatgccctg taccttgagt cactgctaag acagattcct
     8881 tccgcaaatc tatatttgcc cgaaagtatt ctggaaaaac caaaaagtgg aaaaagtgaa
     8941 tatacaatga ggccggataa tcttaagccg cacccgtagc acctgtcgga gtttgttgta
     9001 aatggctcat taataagtat ttaatttttg atatttttaa aagactattt tttaagatac
     9061 tgattacttg tcatttacaa caaactccga caggtgcagg tggaggccga gggctgggat
     9121 gctcggccag tggggctgtc gactaaatta ttattatttt tgcatcataa acctgaacca
     9181 gtaacacctg tcggagtttg ttgtaaactc ttaattttca gacatataaa tttacaattt
     9241 aaaaccaaaa taaaaaataa ataattgatt acaagcgatt tacaacaaac tccgacaggt
     9301 ggaggcttgc tctccgaaaa agagtttgaa gctctttacc ggcagagcta ccgtaaagct
     9361 ttgtactatt gtaatcaata cattgtagac atcgaaaagt ctaaagagat tgtgcaagaa
     9421 gctttcatca acctttggga aaaacgtcaa agtattcata ttgaatccgg caatccggaa
     9481 tattatcttc ttacttccgt caggaatctg gcatacaatg ctataagaaa caagataaga
     9541 gagtataaaa gaaacggaaa agctatttgc attgatgata aaataaacga aaagtcactc
     9601 tcacaagagc cgtgtgactt acttacattc aatgatataa acattgcaat taatacaact
     9661 attgattcca tgtctcagaa aatccgggag gtttacataa tgagcagaga aatggagatg
     9721 acctaccctc aaattgccga aaagctgggc atatctgtca aaacggtaga gtacagactc
     9781 agtaaggcac ttgctttatt tagaaaagct ttgaaaaaat acttacccct gattctttta
     9841 ttttttaata actttttcag gtgaaattaa taatttggtg taattaatat tatggataaa
     9901 attataataa aatatctatc tggcactgca aacgagaaag aaaaagagca attgttgcaa
     9961 tggattgaag agagtgataa gaatgcaaaa ttctttgccg acctaaagca tgcatggact
    10021 ctttccaaaa ctaaaaatga tcctctttcc gacaaagaag tggttcattt tatgaaaatg
    10081 ataaagagag aaaaacagag acacctgcct tcatggtgga ttgcagcagc tgctgctgca
    10141 gttgtgattc taatctcttc gctgttcata agtatgcaaa gaaaaataga atcatacaag
    10201 gaagatattt cttacatgct tacccaagca ggacctcgat atgagtattc aacaccatac
    10261 ggagtaaaag gcaaggttta ccttcctgac ggaagtgctg tctggcttaa ttctggcagt
    10321 aggatttcat tcccaggaaa attcaacggc aagtcaagag agttggattt ttcgggtgaa
    10381 ggttattttg aggtcattcc tgactcgcaa aaaccaatga atgtcaggct ccgctcaggg
    10441 ctaacaataa aagttgttgg cactgaattc aacctatcct cctacgatga cgacaagaaa
    10501 gtatccttac ttcttctcag cggcaaagtg aatattaaca atgctgatgg taaaaaaatc
    10561 tattcggtac agcccaacga aaaaatcaca ataaacctta tcgataatac attattacgc
    10621 aatatcccac aggaaattgc tccaacgata ggctggaaaa acggctggct tatttttgac
    10681 gaggctcctc tcgaagaggt gtttaaaaaa atggagcgtt ggtatggaca aaaaattatt
    10741 gtagaagacc aaagtgttta ttcaaagaaa cttactgcta aatttcatga agagtcaata
    10801 tctcaagttc ttggactgat gaatcaaatc tcacttatca actacaaaat tgttgacagt
    10861 atagcctata tatctaaatt caattaatta atattattta gggggtatac agattttggt
    10921 gtatctactg tcaaataaac ttaaacaaaa tttgtatgcg aaaattatgt ctgtttattc
    10981 tgactttatt ggtttcagcc acattatttt cccaaaatgt cggaattacc ttcaaaggag
    11041 aaaatctccc tttgaaaagt atcttaaatg aacttgaaaa aaagtccgat tttacattta
    11101 tctattctcc ctcgacaata gatgtaaacc aaaagatctc cattacggcc aattctgaag
    11161 attttttttc cctcctcgac agattgtgcc gaattgctaa aatttcctat aagacaagtg
    11221 gaagacaaat tgttctaaca cctttggcaa cttctgaaag ggcactaaac cagcagaatg
    11281 taacagaaaa cggaaaaaaa atcacaggta cagttctgga tgaaaaccga gaacctgtag
    11341 cgggtgcata tgtaatactt caaaacaaca aaaaaattgg cacttataca gatgcaaagg
    11401 gaaagtttga aatcaacatc cccaatgagt atgcaaatga tgctgtcttg cttttttctt
    11461 ttttcggatt aaaagctact gaagttaaaa ttggtgatga taacaatatt tctgtcgtaa
    11521 tgccatttga agaaaaacag ttggatgatg tagtagttac cggctatcag acaatttcga
    11581 gagagagggc aaccggttca tttgccatag taactcaaaa tgttctcaac accaaaatgc
    11641 agacaaatat tattaataaa attgagggaa tggttgccgg tcttaatatt tatcgtggat
    11701 ctgtgcaaat aagaggtgtt tctactatta acggaaataa aaacccactt tatgttgtag
    11761 atggcgtccc ttttgaaggc gaaccaggaa gtaatgcatc tcctctcgac gttattaatc
    11821 ctgcagatgt ggtgaacgtt actgtactca aggatgcaac agcagcctca atttacggag
    11881 caagatctgc aaatggtgta atagtcataa ctacccgaaa tggcgaagag ggagcaacaa
    11941 gggtaaatta caatggatca gtaagcatga taggtcttcc tgacagagga tatgccaata
    12001 aaatgaacag cagtgaactt gtagactatc agttaatgct aatggagact tatccaaaac
    12061 ttacccgtaa gggaataaga gaatatcaga atccagtaca gtctctcatg ctggacaaaa
    12121 aagagggcaa aataactcag gaacagttgg aacaggcatt aattccatac agaaattttg
    12181 acaggtatga tcaggttaaa aacgagtttc taagaaaata ctcaattaaa catcagcaca
    12241 atcttgcact tacaggcggg actaaaattc acaagtacaa cttttccgta aactattccg
    12301 gtactgctcc ttatgaaaag gctcagtatg aagaccgact tggatttaat ctgaaaaaca
    12361 cctttgattt ctttaaatgg ctaagtgttg acgcaggcat tctttacagt caggtaaagt
    12421 cagattatga caacggaata ttgggaatga gctacctcga tgcaggtgga gcttcatacc
    12481 tcagattcag ggaagataat ggagagccat ctcaatggta tcaaaccaaa tctcaatatg
    12541 agatagaccg attgaaagct ttaggtcttc aagatgaaac attctatcct gtaaacgagc
    12601 tgaagaacag aagatacact gcaaaaacaa attatcttaa catcaattta aatgcccgca
    12661 ttaaaatact tgatggcttg acagcaagta tcagatatca gaccgaacag agcaatggtt
    12721 ttaccaaaca atatgacacc aaagatgcaa taagtgttaa aaccatgatt aacaacgccg
    12781 ttcagatggt taacggagta ccgaaatata atattccagt cggtggacaa atcgtacaaa
    12841 aatggatgga taactattct tatactttca gagctcaaat tgattacaac aaacaattga
    12901 acgataataa tattgttcag tttctggcag gtgctgaagc tagaaaagtt gttacctccg
    12961 gaaatggctt ttatcgtctt ggatatgatg acaataatct tggatacagt gagatagatg
    13021 ccctgacact ctccaagatg ctttcaggaa cagagtcgat ttatggtaca ttctcattta
    13081 gcaacaagac tcctgcaagt acatttgtag ataacagata tctttctttc tatgcaaacg
    13141 ggtcatatac tttgaacaac aaagctactt ttactggaag tatcagaatt gaccaatcta
    13201 atctttttgg tactgatccg aaatatcaat atcgtcccct ttggtcttta ggtgctcatt
    13261 acgagctctt aagaaattac aattggatag atagactggc cgtcagagtt acatacggaa
    13321 ttaatggcaa cattcctaaa ctcaatggcc cgtacctgat agctaaagtt gacaggaata
    13381 actactatac caacgaaaac gcaatgtaca ttgcttctcc tcccaatcct cagctaagat
    13441 gggaaaagac aaaggttttt aacgttggag tagattttaa cttgctgaaa aacagacttg
    13501 atggctcaat tgaattttac gacaagcaca ccaatgatct attggggcca tttgccatag
    13561 accctacttt gggatggaca gatgtcgata tcaattttgg cagcatgtac aatagaggag
    13621 tggaaatagc cctgaacagt caaaatatca aaaaagagca ttttagctgg agctcctcct
    13681 tgttgtttag ttacaacaaa aatatgataa caagggttga agaaaccgac gagtctgcct
    13741 acagttatta ttacggcact aatattcgta aaggatatcc aatgggtgca ctgttcagtg
    13801 taagatataa aggccttaac caaaatgggg ctcctgtagg acttacaaaa gacgggcaag
    13861 agattttgaa ttacaatcag ctgacaaagg atgatttggt atactccggc gttgtcactc
    13921 ctccatataa tgcatctttg acaaattcaa tttcatggaa gggatttgaa ctttcattca
    13981 tgtttatcta ttcaggcggg catgtaatga gagacgttgc tgcatcgtac acaattacaa
    14041 gccatccaat gtatgcagtc agcaacgtgg acaaaaatat ggcaaattac tggaaaaaac
    14101 ccggtgatga aaataagcca aatgtcaatc ctgcattcat gtttcagtca acagtaagaa
    14161 atggcgaata tatttacaag gctgcagaca aatttgttaa gaaagcggac tttatcaagt
    14221 tgcgtgatct tatactctcc tataatctgc catcgaaaat tactaaaaag atgggcatag
    14281 gagcagcaac tattaatttc caggcaagaa atctttggtg gtgggctgcc aatggaaacc
    14341 tcgatcctga agtttggaca ggcacatcac tctctcctac cagaggcact aaatatcctg
    14401 ccgaatttat tgtaggatta aatatcaacc tataaaaata ttcaacacca tgaataagaa
    14461 aattttaata aatatattgg ctgtatcact tctgatatct gtaacaggct gtgacgacta
    14521 tttgaacaat gtgcccaaag gcatgactat tcctcaatat atggaagatt atcagaagct
    14581 gttaaactct cagtccctga ttaatgcttc cgacggaatg ctcgattacc ttactgataa
    14641 catccatctg cttgataaaa atgcaactgc ttcatattat atatacataa ataaggatga
    14701 atccgtaaga aatctttatt cctttaaacc aggacaggtc tatgttgaag gcacaaagga
    14761 ttatatatgg aataatgcct acgaaagact ttttactctg aatactgtca ttaatgaagt
    14821 catggattct aagggaggaa gtgaagaggc aaaattaaga ataaaggctg aggctctttt
    14881 tggaagggct tatgaatact acaacctggt aaacacctac gctaaacagt ataacagtca
    14941 aactgccagc aaagatttag gtattcctta cctcactgaa gctgacatta accaaaagta
    15001 tcaaagacat actgttgccg aagtttacga aaaaatgctt gcagacttgg atgaagctgc
    15061 ttctcacata cccgatatta cagctaacag ggctcatccc aataaggctg cgttgtattc
    15121 tttttatgca aaaatttatc tcacaatggg agactacact aaggctctca aatatgctaa
    15181 tgacgcactc gctctcaaca gtgcactgct caacttgaat aattacgaca aagcagaagg
    15241 tacaacatgg ggaagagtct acaaaaaagg aaatccttct gaacgacttc ctgacattaa
    15301 ccatcctgaa gcaaattatg tgaagtggct gtctggttct cttcaaggta gtgtgatgct
    15361 tagtagtgaa atgagagatc tttacaaaaa ggatttaaat ggcgcaactg acttgagaaa
    15421 ggaatatttt ttcgccgaaa atcaggttaa tcttggtgga agtactgact atttcccggg
    15481 agagtgcgca tttgtactct acgcaaactt caatgtagga tttacttcta cagaaaatta
    15541 tttcatagca gctgagtgcg aagcgagaat tgggtcgaaa gacaaggcca tgcaacttat
    15601 caatactgtc agaaacaaca gaatgatgaa taatgaacag cttactgcat cgaccaaaga
    15661 agaagcttta atcaaggtac ttgaagagaa gcggagagag ttttgtttca aaggtccaat
    15721 gaggttgttt gatcttaaac ggcttaatct cgaatcacaa ttccaaaaaa cgataactca
    15781 tactgccgat ggagaaacat ttatcctgcc tccgaacgat gtaagataca taatgcctgt
    15841 taataaggag attcttaatt tcaaccctga ttttccggta tacgaaagat aaaaacaaag
    15901 cgaaatatga ttataaaaca atgagaaaag tttattgtca gatctttata tttgcagcaa
    15961 tgtgcctcta tttttcacca ctctctgccc aaaaggctat tcgcttcgaa aaaggcaact
    16021 ggaatgagat tgtggaaaag gcacaaaaag cgaaaaaaat gatttttgtc gacttttaca
    16081 ctcaatggtg tggtccatgt ttaaatatga gtgaagaggt tttcagtttg ccggctgttt
    16141 acaattttta caacactaat ttcattaatg ccaaaattga tgctgagcac ggtgaaggaa
    16201 aagaacttgc ggcaaaatac caggtcaagg tttatcctac atatcttttt atagatccaa
    16261 caacaggaaa tgctcttcat aaaagcagtg gccgacagga acctgatact ttcatcttta
    16321 ccggtgaaag tgctcttaat ccaagactca ggtctttcta tctcgaggca aattatgata
    16381 aaaataagaa tgatctatca tttttaaatg attatgccaa atacaagagt tcaatatacg
    16441 accgtgaggg agtagccaaa gtccttaaac aattggaaaa acttgacgtc aaactggaca
    16501 attcactggt ctgggaactg tttgtaaata atattacagg ctatgacaac tcctttattt
    16561 gtgaagttat cacaaattat gacagatatg ttaaactata cggcaaggaa gatgtcgaca
    16621 acaaattggc caaagagaca tcatatgctc ctgttgatta tcttaaaggg ttacccgatt
    16681 ttaaaggaaa gaacacaaat attgtaatca acgaaattaa cacggcacaa agagatgcca
    16741 attacgatga agcaataaga ttgattgaca tagctcttaa agatacaaca attgatcgca
    16801 ataaatttct taatgctctt agatttactg ttagattaaa tgacgggaag aaatatcctg
    16861 acttgtgggt aaaaaagtgt ggggagtata tcaggtacat cgcttacaat tactctgaca
    16921 ggaaagatcc ttatattcat tatgaatatg ccctgtacct tgagtcactg ctaagacaga
    16981 ttccttccgc aaatctatat ttgcccgaaa gtattctgga aaaaccaaaa agtggaaaaa
    17041 gtgaatatac gatgaggccg gataatctca aaccaaaacc tcacctgtcg gacaagtaaa
    17101 ctgagccagc gcctcatcca ttttggattt tatctcgaaa gtgcacagat tgcctatact
    17161 ccctttatga gtatgggcaa tcttcttttc aggaacaaaa cggccactgg caacttcttc
    17221 tcccaccctt ttataggctt ctctgaatgg cacaccctca agaaccaatc tgttaacctc
    17281 ctctacagtg aacagataat catatttaga atcttcaagt atttcccgat ttattttgat
    17341 actcgagagc attagcatgg acatctttat gcagttatgc attctgtcca gtgccggaaa
    17401 aagtatctct ttcaacaatt gaaaatctct gtgatatcca tgaggaagat ttgtcgcaag
    17461 catggagact tcattgggaa cactctgaat tctattgcag ttagccctta ttaactccca
    17521 aacatccggg ttctttttgt gaggcattat acttgagcct gtagtcaatt catcagggaa
    17581 agagataaac ccataattgc tgttcatgta catcgtgcag tccacggcga gtttgttcag
    17641 tgtagatgct attgatgcaa gagcgttggc aactgctttc tctgacttac ccctactcaa
    17701 ttgtgcagaa attgagttat aattcattgt actgaaacca agcagtttgg tagtcatatc
    17761 cctgtcaatc gggaatgaag aaccataacc agcagcggag ccgagagggt tttgatcaac
    17821 cactttccat gctgctacaa gaagatagac gtcatccaca agtgcctctg cataagagcc
    17881 aaaccatagt ccgaatgaag aaggcattgc cacctgataa tgtgtataac caggcattaa
    17941 cacatctttg tgcttctcgc ttaaagattg aagaagttta aaaagatctc ctatctcatt
    18001 tttaaactgc aaaatttcat gcttaaggta aagtttaatg tcgacaagca cctgatcatt
    18061 cctggatctt ccggaatgaa tttttttacc tgtttcacca actttttttg taagatagag
    18121 ttccacttga gaatgaatat cttccacgtc aggctcaatt ttgaattctc ctttattaat
    18181 ggtatccagt aaatactcta gttcgctttt cagaatatta aattcttcgt cttcaagcaa
    18241 gccaacactg ttgagcatag ttatatgggc aattgaacca ataacatcat attcagccaa
    18301 tctctcgtca aattctctgt cgctaccaac agtaaagcgg tctaccagcc tgtcctccga
    18361 taatactccc ttatcccaaa gtttagccat aattttataa ttttaatttg gaaattaatt
    18421 taatatatcc ctttattccg tcttctatct cccgtgtcca aacaaattcg tcagctctgt
    18481 gcgatctcga agagtcaccc gggcccatct ttacggctgg aaaatcaagc ctcatccagt
    18541 ctgatgtcgt aggagatata tatgttttaa taccacaagc ctctgctgct gcaatcaaag
    18601 gatgatcgca aggtgtcaac gacgatttgt ttgtaagaga acgtgcggta agttcactct
    18661 tcatgtgagc cctcaaaata tccataatgt cactgtttgt ataacagtct gtaggtctta
    18721 tatctacgac aaatttgcag ctatcaggaa taacgttgtg ctgagtgccg gcctcaattt
    18781 gagttactgt acatctcacc tctcccatct tttcagagat caaagggaac ttataacttt
    18841 ttaaccaatt aatgtcatca agagcaatca ttatggcatt taggccttca ttccttgccg
    18901 catgcccaca aattcccctt gccagagcat ctacaacaag tagtcctctc tctccaatag
    18961 ccatattcat ggaagtaggt tctcccacta ttgcacagtc cggcctcata atgtgcctca
    19021 aggcaagttt gattcctctc tttccagagg tctcctcttc agcggaaata agcaacatca
    19081 gattaaatgg aagtggttca tctcgctgtt caaaaaacaa aaaagtctga atcatggaaa
    19141 caagagaggc tcctgcatca ttgcttccca acccaaaaat cctttgcgga tctaactgag
    19201 ggtcgtatgg atcaaaagaa tagtgcccaa cagctctgac tgtatccata tgagaattga
    19261 gcaaaagaat ctttttggca gtatcgtacc ttctcgaatt tactattatg ttgttggaaa
    19321 tcctttcagg tgtatacccc tttgaagata aatagttgca caataaattc gatacttcat
    19381 tttcctcccc tgaaacagaa ggggtcgaaa caacctctct aagcaaagag attgcttcgc
    19441 taaaatattt attatcaacc atagctcgat gtttatggtc tcaaaattgt ccctttgtta
    19501 ttattcaagt taagggcatg cttgatatat acctcagaaa ctccattctc aatagcagca
    19561 aatgcagttt caagttttgg gagcatacct cctgatacaa ccttgctttc ttttaatgat
    19621 tcgaaacttc ccctcgtaat taacggaatt actgaagaat catcttccgg gttagaaagt
    19681 actccctctt tttcaaagca atataccaac ttggtggagt aatctctcga aagggcaatg
    19741 gccaggctgg atgccatggt atcagcattg gtattgagca atgaaccgtt tctgtcatga
    19801 gtaattgcac aaaatactgg agtaattcct ctgccgagaa ggctatcaag aagtggaaga
    19861 ttgatgtctt caggattaac gtctcccaca aaaccaaaat caattggata gggagacctt
    19921 cttgtggcag gaacaatgtc tccatcagca cctgaaaggc ctatggcatt agtgccgaac
    19981 ttctgaagca atgcgactat gcttttgttt atccagccgg cataaaccat tgtaacaagc
    20041 ttaagagtat ctatgtcagt tactcttctt ccatcctgca tatttacttt aagacctatc
    20101 tttgaagata gctctgtggc catagcgcct ccaccgtgaa caagcactct cggtccactc
    20161 tctttgccca actgctttaa aaattcttca agagcagccg aacgatcaac aatatttcct
    20221 cctattttga caactgtaag ctgtttcata atattcaatt agttaagtta tagattttaa
    20281 aatctctctt agcactacct gggcagaaac cactctgttt gcagcttccg ggataacgat
    20341 tgagtcaggt gattctatga cctcatctgt tacaatcata tttcttctta caggcaggca
    20401 gtgcatgaat ttggcgttat ttgtaagttt catctttcgt gcatctacag tccagtttct
    20461 atctttgttt acaacttttc cgtagtcagg gtctctaaat ttagaccagt tttttgcata
    20521 aataaaatct gcgtttttgt aagctgcttc cacatcatat tcgattttgg cacctttggt
    20581 aaactcttcg gagagttcat agccctcggg atgagtaatg acaaattcat agtctgtcgc
    20641 attcatccac tccgcaaaag agtttgccac tgcctgtgga agcggcttgg ggtgaggagc
    20701 ccacgaaaga acaaccttgg gtcgttcaac tttcttatac tcctcaatag taattaaatc
    20761 tgcaaaactc tgaagcggat ggcgtgttgc agattccatg ctgaataccg gtcttcctga
    20821 atattctata aattgattga gaactatctc gctgtagtcg taagatttat cctcaagctt
    20881 ggcaaatgac cttacaccca taatatcaca atatgagccc ataacaggga ttgcttcaag
    20941 tatatgttca gacttatctc cgtccattac tacacccctc tctgtttcaa gtttccacgc
    21001 tccttgattg atatccaaca caatcacatt cattccgaga ttcattgcag ctttttgagt
    21061 acttaaacga gtcctgaggc ttgaattgaa aaaaatcatc aataaggttt tatttttacc
    21121 taaagcctga tctgcaaatg gattggcttt aacctcagta gcttttgcta aagcaatatt
    21181 taacggctct atatctttaa cacaagtgaa tttattcatt attatctatt taactggtag
    21241 gtaattgatt ttaaaaaata ttctgcctct tccatcgaaa tcgtaagtgg aggtaaaatt
    21301 ctgataatat ttgatttaga gcttcctgta aaaatatgat tctcaaataa aagtctgtct
    21361 ctcacccctg tatgtttact atccaattct attccaatca ttaatcccct gccacgaaca
    21421 gaagttattt ttcccggaat atcggcttgt attttcttaa gggatgcaat caaccattct
    21481 cccactattg ctgcattttg tatcagcgat tctgactgaa gtacatcgag aacagcaata
    21541 gcagcggtac aggccagatg atttcctcca aatgtacttc ccagcatgcc gggtaccggt
    21601 tttatttcag atgagattat tactccacct attggaaatc cgttgcccat acctttggct
    21661 gttgttatga tatcaggctt gattccggaa tgttggtgag caaaaaattg accagttctg
    21721 ccatagccag attgaacttc atcaagaata agaaaggctc cgaatttcct ggtcaataaa
    21781 cttagctctt taaggaacga agcatcaggt tcgtatattc ctgcaactcc ttgtatcccc
    21841 tcaattatta cagcagcaac atcccccttg gagagttcat tttcaactgc agagactgaa
    21901 ttaatcggca caaatgtcac ctcatgtccg gcattaaaag ggctaacata atttggattg
    21961 tcagttacgg caaccgctcc tgatgtcctt ccatgaaatg cctccttaaa tgaaacgact
    22021 cttttcttcc ctgaggcaaa tgaagcaagc ttgagggcat tttcattagc ctccgcgcct
    22081 gagttacaaa gaaacagcga ataatcatca tacccactta ttttccctaa cttgtcggca
    22141 agctcgtctt gtaaagaatt ttgtacagaa ttagaataaa aactgatctt ttccatctgc
    22201 tcacaaagca tttttacata gtaagggtga gcatgcccaa tcgagataac tgcatgtcca
    22261 ccataaagat ccagatattc tattccgtct ctgtcccaca ctttgcagcc tttagccttg
    22321 acaggctcaa caggccaagt tttatatacg ttaaacggtt tcatatttaa aatgcgcttc
    22381 cttttaagct aagagcgcag ttttcttcat aaccgaacat gatattcata ttttgaatcg
    22441 cttgtccgga tgcacccttg ataagattgt caataactga tgtcaaatgc cagtaaccat
    22501 tatgtttctc caaatgaatt aaggctttat tggtattcac tacctcttta agtgaaatat
    22561 tctcgtcgga gattatcaca tagggagagt ttttgtaaac agagttgaaa tgttcaatca
    22621 tttcacttaa atcaaacctc
//
LOCUS       DAOTQL010000013        22542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2335_length_22542_cov_5.271890, whole genome shotgun sequence.
ACCESSION   DAOTQL010000013 DAOTQL010000000
VERSION     DAOTQL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2335_length_22542_cov_5.271890"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="P5293_01615"
     CDS             <1..1301
                     /locus_tag="P5293_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR48663.1"
                     /translation="SMTASGTNLISYTIRLSDATERERSIFEISDEMRQDLANIPEVY
                     KSTVSPGGGRSSSAVGMGSSTFEVDVIGYNMTDAEATANQIKQIMENTAGLRDIQLSR
                     EDYSPQLKVIFDREKLAMNGLTMTSAATVVRNRINGFTASTLREEGDEYDITVLNSKK
                     YRSEIDDIKNILIYNSQGKGIRLSEVADVREGFAPPSIEHLDRERVIKVSGSIYNRPL
                     GDIAKDMKAQLAKINYPPQITAEISGSVEDQQESFNDLYTLLALVIMLVYIVMAAQFE
                     SFRDPFIIMFSLPFAFTGVFLALWMTGTSLSIIALIGSVMLVGIVVKNGIVLIDYINL
                     NKERGASVRRAVISGGRSRLRPVLMTTTTTILGMIPMAIGIGEGSEIWQPMGIAIIGG
                     LTISTILTLIVIPCIYTSFHFGDMKRKRKKFAEDLSIIDN"
     gene            1303..1596
                     /locus_tag="P5293_01620"
     CDS             1303..1596
                     /locus_tag="P5293_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48664.1"
                     /translation="MKAVMIAYNQAHNAEVQEILDKCNIRGFTKWEDVQGRGSKDGEP
                     HYSTHAWPSKNMVTITIIEDNKLSELIKQLKFLDDRAEKQGLRTFVWEAQQAW"
     gene            1660..1977
                     /gene="trxA"
                     /locus_tag="P5293_01625"
     CDS             1660..1977
                     /gene="trxA"
                     /locus_tag="P5293_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRR48665.1"
                     /translation="MAIAVNDSNFNQIVINSDKPVLVDFWATWCGPCRMVSPIVDELA
                     AEYEGKAVITKCDVDSSSDVPLKYGIRNIPTILFFKNGEVKDKIVGATTKSAIVAKLN
                     ALM"
     gene            1985..2575
                     /locus_tag="P5293_01630"
     CDS             1985..2575
                     /locus_tag="P5293_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRR48666.1"
                     /translation="MKKVFVLLISVLLFSTLSYSQTRFGIKGGLNFTNISNLSSDVEK
                     TWKNQTGYQLGVALQIKIPVIGLAIQPELLYSTVKSASDNGNEINLDYLTLPVNIQLG
                     TDMLFFRPFIVASPFVSYMIDNNVRLEDQPVDEINRFDYGVGVGVGIDIWKLQIMGKY
                     NWGLGKIENAGDEWDQSETYKDATLQGFQLSIAFLF"
     gene            2694..3671
                     /locus_tag="P5293_01635"
     CDS             2694..3671
                     /locus_tag="P5293_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRR48667.1"
                     /translation="MESFDIISDIGEEFHRYRALMEDSLNSRSSLLNAINTYQLQNSG
                     KQLRPILGILSAKACGGTVNDRTISCVAVAELIHTATLLHDDVADKSDRRRGVKTVQA
                     EYTPAASVLTGDYWLSKAMTILVNLFDRQIMEFYTNAVVNLSEGELFQMQKASSLDTS
                     KSDYFNIIYSKTASLFIGAVKSSVATVCDKEEVLMNMEMFAENLGIAFQIRDDIFDYM
                     PSLNTGKPTGGDIVERKLTLPLISALDLAAEDEKIRLKRMLRDRKTNDNRLVQEAFRI
                     VEKYSGIYLAQQELLKYCQRAEKALSFLEESSYKRDLVTLAKYVGERLY"
     gene            complement(3668..4252)
                     /locus_tag="P5293_01640"
     CDS             complement(3668..4252)
                     /locus_tag="P5293_01640"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRR48668.1"
                     /translation="MNYSPIIAIENILVSPKIITEYFCCDYSKCHGACCIIGDSGAPL
                     EKNECSILAGEFQQFSKYLTVEGIRSVKEQGPFVIDADGDAVTPLVNGEECAYSWFDK
                     DNNCLCGIEKAFDAQCCSMKKPISCWLYPIRVSKLSNGMTALNLHEWYLCMDAFVKGK
                     REGIPVYKFLRDPLIFAFGKDFYKQLEEAALRLF"
     gene            complement(4254..7061)
                     /locus_tag="P5293_01645"
     CDS             complement(4254..7061)
                     /locus_tag="P5293_01645"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRR48669.1"
                     /translation="MKKIFTLAVTLVMAFLASAQMPQTLPLDPKVKTGKLDNGLTYYV
                     VKNSEPKGQADFYIVQKVGSILEEENQRGLAHFLEHMAFNGSKNFPGNSIISYLEKIG
                     VKFGANLNAYTSIDQTVYHIANVPLKRDGIIDSCLLILHDWSCGIALTDKDIDDERGV
                     IREELRTSSNASMRMLEKVLPEIYPDNRYGERLPIGLLDVINNFTYQEIRDYYNKWYR
                     PDLQGIIVVGDIDPVAVENKIKSLFGSIPKKENPAERIQFPVEDNKEPIVAIASDPES
                     KSTEFYLMYKHDVVPVELRPTVAQTVIDYMNNMVCSMLNSRLSELVQKSDPPFVGADA
                     SYGDFFVSQTKKAFTLSVEAREGELERGARTIINEAERMRQFGFTASEYERAKANYMS
                     RLERLYKERENQKNDYYVQNLVNHFLTGEALPGIEMEYTLMQQIAPAITVDQINTYAK
                     SLPTRENVVMLVMMPQKEGIKVPTKEEILSLYESALNDKLEPYTETVSNEPLVAVTPK
                     AGKVVKETREPITDALVWTLSNGATVVIKKTDFKEDQILFTATSRGGFSLTNPEDVVI
                     TKVLDDIATLGGLGKFSAIDLKKNLAGKNVSINPSIGINNEQLSGSSSPKDLETFMQL
                     IYLSYTDVRKDDEAFDSFITRMRAQLVNMESLPMIAFQDSLSKTLFNNNPYVNRLTSK
                     MLDQINYDRALQIIKERFANAADFTFVFVGNIDPATVKPLVETYIGSLPANKAKKEDW
                     KNLNNVPVRGVISKQFDKEMQTPKSTVYKIYSGKIPYTVENKIMADITKQIFDQVFTR
                     TIREDEGGTYGVGVSMSVTYYPEDYFVFLYGFDTDVAIKDRLIARAQKEIENVMKNGV
                     EKTDFAKVIEYMQKNYTQNLRENNYWLSVLTNRYFLGKDMHSTFEATLKSMTPEKIDN
                     FIKTALTQGNEVEIIMNGFKK"
     gene            7186..8145
                     /locus_tag="P5293_01650"
     CDS             7186..8145
                     /locus_tag="P5293_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR48670.1"
                     /translation="MKKIVTILSTCMLSLFSFTFVYGQDIAAAIETYNAGATALASNN
                     YVAAIESFNKAIKMFEAISELDEDGASAFKQCKEILPQIHLRYGKELAVNKNFDNALA
                     QLDKAIETAKKYNNPDVEKEANELIPQLVMAGADDNLNLGLFNEAIAGYNKMLTYTPN
                     DPDVYLRLGIAQSKLNNEDAAIAAYLKAIELGDKQDAPKMLSNIYLKKAAAAFSDKNN
                     SEALAMAKKANQYYETVQGNQIIGLSAMQLKKYDDAIPALEAYIAQSNDSKNKNAIIY
                     NIAAAYEAKGNIAKACGYYKQIVSDPKYKAAVEYKINTVLKCK"
     gene            8136..8315
                     /locus_tag="P5293_01655"
     CDS             8136..8315
                     /locus_tag="P5293_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48671.1"
                     /translation="MQVTTLELLAPAGNKDIGIAAIDCGADALYIAGSKVYLTLNTII
                     FDNELGKGLTAETVK"
     gene            8346..9473
                     /locus_tag="P5293_01660"
     CDS             8346..9473
                     /locus_tag="P5293_01660"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRR48672.1"
                     /translation="MKFSDIVGQNSLKQQLIKLFKEGRMSHAMLFCEEQGYGALSTAL
                     AFVQYNLCLNKSAEDSCGTCPTCNKIQKMIHPDLHFAVPVNSTKKITADKKPVTDLFL
                     DDWRRAISENPYITEQEWYDLIGIENKQGIIGVNEAAQILKKLSFRSFEGGDKYVIIW
                     LPERMNQEAANKLLKLIEEPPAGTYFFMISHSPERIIPTIVSRCQIISLMPIEREMLA
                     QRLAEEFKISEEEAFFWSRISGGSLSKAREIINSDRETVEDHEIVVKLLSACCSKDMP
                     EVISLWERLSAMNREKQKSFCHYALEFLRELYMTSLGLFDISNTPVRRRENVVSLSKR
                     INPSFYIKAYEILNDALQDIERNVNAKYIFADMSNRFFLSL"
     gene            9479..10636
                     /gene="ricT"
                     /locus_tag="P5293_01665"
     CDS             9479..10636
                     /gene="ricT"
                     /locus_tag="P5293_01665"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRR48673.1"
                     /translation="MKDQKIINDCSRGCKIWKDSDDTLNVKYDNNCCKLSVFDWLQGI
                     SDEKSKDIFEVRFKNTRKSYFRNASDQQIDVGDIVTVETTNGHDVGIVTLAGPVVLIQ
                     LKRNNIDIDHFEFKKIYRKSKGVDIERWQDAISREHKTMIRARQIAASLGLNMKIGDV
                     EFQGDGTKAIFYYIADERVDFRQLIKLFAEEFKIRIEMRQIGARQEAGHIGGIGVCGQ
                     ELCCSRFINDFKSITTQAARCQDLSLNPQKLAGQCSKLKCCINYEAAVYIDAHSSMPI
                     VKAPLETEGGPLYLMKSDVLKGIMWFSYEESSISDMFPLTFECVREVLRQNTRGIKVP
                     DLTPDSDSGPEFKSAVGEGSISRFDEERKANHLNRKKRNKQSSGRNNENQK"
     gene            10620..11048
                     /locus_tag="P5293_01670"
     CDS             10620..11048
                     /locus_tag="P5293_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48674.1"
                     /translation="MKIRSKVLLLFFFGALLCSCAKDDYEPSVQWFKFNCRNTQRSFN
                     VDFSLRISVTYKDETLPVMLEILSPSGIRYRDTLNIPVKKQVSDGLTTVVRSGVWRDV
                     LWHYRKNVSFPLTGRWLFTIYYLEPGTKKGKIKEFKLKIQ"
     gene            11057..11752
                     /gene="trmB"
                     /locus_tag="P5293_01675"
     CDS             11057..11752
                     /gene="trmB"
                     /locus_tag="P5293_01675"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRR48675.1"
                     /translation="MGKDKLKRFAENKTFSNLYQPDFSEVFRKDYRLKGKWRREVFGN
                     DNPIVLELGCGRGEYTVEMARRNPCNNYIGIDIKGARLWRGAKSALEEGLVNVVFVRC
                     RIEFVESLFGENEISSVWITFADPQIKRENKRLTSPFFLDKYAKFLINGGTVHLKTDS
                     LFLHEYTLGIVKERGYEIIESNPDIYGTGYAGQDNMLDIKTHYEKIFLSQGLPITYMS
                     FKISRDSLGRRTQ"
     gene            complement(11712..12200)
                     /locus_tag="P5293_01680"
     CDS             complement(11712..12200)
                     /locus_tag="P5293_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48676.1"
                     /translation="MKSGFDFKKYFIRVVKYLIYFVIIFSLIIAIFALASRQELSYSA
                     LFRPGTGVQLLVFFVVISLAYPLVSFINKRAYLNHSFAEERDKILKVFEDSNYIKVAE
                     DATTITFRHKSKVTRMFRMFEDTIVVDFSDNPIVLKGMRKDVYRLAKSIEYASQGNPE
                     KS"
     gene            12308..13174
                     /locus_tag="P5293_01685"
     CDS             12308..13174
                     /locus_tag="P5293_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48677.1"
                     /translation="MEKERTLRNIVIALSVIALLLIGVLAYIWIDRSRIIGDLTVEKE
                     QLTQQTEQLKEEYAMLTTNNDSLNVQLSKEREKVEQLIDRLKRTEATNRTKIRQYEQE
                     LGTLRSIMRGYIIQIDSLNTLNISLRKEAAAAKDEAKETRQKYEELQTTTNEYALKIQ
                     KGSMLKGRGINVVAITASNKETDRSSRTVKIKTCLYLVENNIATKGPRKIYIRVKGPD
                     GILMTDGSQQIFNVGGEQMIYSAVREVDYQGSELEVCIYFAGSAPFVKGVYNVDVYTE
                     EGKLGTADLLLK"
     gene            13180..14325
                     /gene="hemW"
                     /locus_tag="P5293_01690"
     CDS             13180..14325
                     /gene="hemW"
                     /locus_tag="P5293_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRR48678.1"
                     /translation="MAGIYIHIPFCSQFCIYCDFYSIRNKKDRVEKYIASLLCEIEQR
                     SDFFKNCKIKPTTIYIGGGTPSLLSSDNLSKIVKSVYAQFGIDTFSQIQEFTIEVNPD
                     DLTKDYLIQLKDIGINRLSIGIQSFFDEDLLWMNRRHDSCKAIDAFFMARDCGFTNIS
                     IDLMFGFSLLTEQKLIANLERLISIRPEHLSLYQLGIEKGTPLGKSYECGEYVPLSDD
                     NCSLQYSLIQNMLSKAGYIQYEISNFSLPGKEALHNSSYWEHKPYIGFGPSAHSFDGK
                     IRSWNCSSLSKYCSFLQVGDRCFKCENLSPRNIFNEKIMLSLRRTVGADLQVLRNGTS
                     PEIYSLFQQQLENALKRGEVIMDGNKIKIPPDRFFVSDGIIRDLMVV"
     gene            complement(14338..15000)
                     /locus_tag="P5293_01695"
     CDS             complement(14338..15000)
                     /locus_tag="P5293_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRR48679.1"
                     /translation="MSNIFTSSIGKKLIMSITGLFLMLFLLVHLFANSAYLFGPEAFD
                     TIIIIMSSPVVVAIVPVLAAGFIIHIFYASYLTITNLMARGKERYAVAHKGASDSWAS
                     KNMFVLGVIILGFLIFHLTHFWAHMQLKDFTGREPENVNNLMQLTFSNIWIVILYLIW
                     FFAIWFHLTHGFWSAFQTLGMNNSKWLKRWQVIAYIYATFIFLGFATIAITAYCRSKG
                     IC"
     gene            15136..15849
                     /gene="pyrH"
                     /locus_tag="P5293_01700"
     CDS             15136..15849
                     /gene="pyrH"
                     /locus_tag="P5293_01700"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRR48680.1"
                     /translation="MKYKRVLLKLSGESIGGRIGVGLDENILSQYAREIHDTVALGVE
                     IAIVIGGGNIFRGLSGVGKGFDRVKGDQMGMLATIINSIALSMALKSQGTPAEVFTST
                     PAEPYGIYYGRDKAVKFMESGGVSIIAGGTGNPFFTTDTASALRAAEIGAEALLKGTR
                     VDGVYSADPEKFPDAFKYISISFDKALEENLKIMDLTAFTLCKENSIPIVVFDVNIPG
                     NLVKVVNGENIGTLISYDN"
     gene            15867..16427
                     /gene="frr"
                     /locus_tag="P5293_01705"
     CDS             15867..16427
                     /gene="frr"
                     /locus_tag="P5293_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRR48681.1"
                     /translation="MNNQKEVIAAAKEKMIKAIFHLEEELKSYRAGKANPAIFSNILV
                     DYYGTMTPIPQVASITAPDAKTMFIQPWDKKMIPLIEKAIMAANIGFTPQNNGEHIRI
                     NVPPLTEERRRELVKKVRQEGENAKISIRNARRDGVELLKKYQKDGLPEDVVKDGESI
                     LQKETENFNRRVDEIILAKEKEIMTV"
     gene            16430..17839
                     /locus_tag="P5293_01710"
     CDS             16430..17839
                     /locus_tag="P5293_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HRR48682.1"
                     /translation="MSAKIGRVRFYLFFGILILSFWVISCKAPQIEGPQESYLPSEIA
                     PSLSELPLRIELDVKKIESVVNASMNGLLYQGDKLAGEDLSVKIWKGGQFMFFVNNNE
                     ITYKVPLKVWCRYGWKVEKFGVSFSDHYEATGTISLVYKTAVGIDENWKLVTKTTPAG
                     YEWLEKPKVKAIGVSIPVTPIANIALSYFQKTITEQVDKILFENVPLDKYVGAVWDEV
                     QQPYLLSDEDNLWIRITPKEILLAPFTTKGNKLNITLVFYAQVESVFGAQPVVKNKVP
                     LPSLRYIERQPQQFSINVGADVTFDKIAEFARIQLQGKSFTESGKIVKIDNISLYSSN
                     GRAVVVADLSGSYKGRIYFTGKPAYNSEKQSLEIIEPEFDIKTQNALIKSANWLLHGM
                     ILKKITPMLTYPLEETLKTLKDQANSMLKSYEVYSGVHITGSLDSLAVTTVSMVPGAV
                     RVGAEIKGNVALIIDELKF"
     gene            complement(17836..19488)
                     /locus_tag="P5293_01715"
     CDS             complement(17836..19488)
                     /locus_tag="P5293_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRR48683.1"
                     /translation="MKKYTSYLIIASLCLFTFFVNNGFLFTEIMESRNIITAREMVYD
                     NHWMVPTMNGEIRLQKPPLPTWLAAVAEMIAPDNIAVQRSMSALAATFLVLMLYLIAV
                     ELTSNKRYGLISTILLCTSYNIIMAGRTASWDIYCHAFMTGAILFLIKAVRKNEGGTG
                     LFAISGFLTGLSFMSKGPISLYGLFLPFLISYFVIYKNSLRGKWKGIAIFVLSAIIVG
                     GWWYLYVYLSHPEQFLKIVGTESQNWTNYHTRPWYYYYDFAFETGIWTLLCITSLFWG
                     YWRKKVNHPKGYSFALLWMILVIVCLSILPEKKTRYLLPMLIPAAMTMGYIFDYWASA
                     VKEKLNYKKWIYYLNTLPIAIMPFIIPIGIYLLILNEGAPFHDVKLSILNFSFITVVS
                     LTIGIYMIKKSIVKPDPMKFLGGIAALFVLVSVSLMPLIAGAFNNPEFNGLDRTRNMP
                     EVANLQFYYDSSKELRIEQVYDAHRIIKPLNIKDSAAVVSAMPCVILTHGMADTLLAP
                     NIKDQVKIKYVGQFDGNRGRKKSKFNKQVHTWDLSIIEPKNN"
     gene            complement(19501..20025)
                     /locus_tag="P5293_01720"
     CDS             complement(19501..20025)
                     /locus_tag="P5293_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRR48684.1"
                     /translation="MEIKYSLEILFERIGRWICKIIDHFYPLFRKTMPIQFFRYGVTG
                     VVNLVFDWILYFVIYNFVLQKKMLHLGIVTLSSHIAAFVIKFPIVLLSGFLLQKYVTF
                     TESNLKGRRQLFRYLIVYGINILINYFGLKFFVDLLHIFPSISNMIVSIITVFVSYFL
                     QKKFTFQIINSTKS"
     gene            complement(20088..21518)
                     /gene="aspA"
                     /locus_tag="P5293_01725"
     CDS             complement(20088..21518)
                     /gene="aspA"
                     /locus_tag="P5293_01725"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRR48685.1"
                     /translation="MKKYHIKSEGTKRIEHDLLGDKEVPAEALYGVQTLRCIENFDIS
                     RQLLSDYPQFIKAFGMVKMAAVLANHRLGLIDTSLKDAIVAACQELIDGKHTEQFPVD
                     MIQGGAGTSMNMNANEVIANRALEIMGKEKGDYQYCHPNDHVNMAQSTNDAYPTAMHL
                     GLYMVHLDVKAALEKLIDSFAVKETEFAKVIKMGRTQLQDAVPMTLGQSFGAFASAMR
                     HELKHLENAAKELLIINMGATAIGTGITAEPGYAEACTQFLKEITGWKIKLADNLIEA
                     THDTSPMVSYSSALKMIAIRLSKICNDLRLMSSGPRTGLAEINLPPKQPGSSIMPGKV
                     NSVIPEVVNQVCFRVIGNDLTITMASEAAQLELNVMEPVLVHSLVESSLWLKRAFDTL
                     RVECIDGITANADHCRYLVEHSVGIVTALKPFIGYTNSTEIAKEALESGCSVYQLVLD
                     KQLLTKEQLDKILDPANMIRPVKITL"
     gene            complement(21623..22345)
                     /locus_tag="P5293_01730"
     CDS             complement(21623..22345)
                     /locus_tag="P5293_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR48686.1"
                     /translation="MINKLSIVIPAYNEGTTIHLILDKIKDVSLINNINKEIIIINDC
                     STDNTEEAVKLYMKLNPEMDIRYHRHTKNMGKGAALHTGIALASGEYTIIQDADLEYD
                     PQEYNDLLKPVAKGVADVVYGSRFMGGNPHRVLFFWHTIGNKFLTFMSNMLTNLNLTD
                     METCYKLVDTKILQSLKLKENRFGFEPEVTGKLARIPGIRIYEVGISYYGRTYREGKK
                     IGWKDGFRALYCIFKYRGYHKG"
     gene            complement(22342..>22542)
                     /locus_tag="P5293_01735"
     CDS             complement(22342..>22542)
                     /locus_tag="P5293_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48687.1"
                     /translation="AENALLNSLKIVPDYYSARVDLMQIYLKLQDWDKLKRETEMMQR
                     DYPNDPFTMIFAELLKSKQVTK"
BASE COUNT         7159 a         3850 c         4582 g         6951 t
ORIGIN      
        1 cttcaatgac tgcttctgga acaaatctaa tatcatatac catcagactt tctgacgcta
       61 ccgagagaga gagaagtatt tttgagatat ctgatgaaat gaggcaggat cttgctaata
      121 ttcctgaagt ctacaaatct acagtgtcac ctggaggagg acgttcttca tctgcagtgg
      181 gtatgggaag ctcaacattt gaagtagatg ttatagggta caatatgacc gatgctgagg
      241 caacagccaa tcagataaaa cagataatgg agaatactgc aggtttgaga gatattcagc
      301 ttagtcgaga ggactattca cctcaattaa aagtcatttt tgatcgggag aagttagcca
      361 tgaatggatt aacgatgaca tcagcagcga ctgttgttag aaacagaata aatggtttta
      421 ctgcttcgac cttgagggaa gagggtgatg aatatgacat tactgtactt aactccaaga
      481 aatatcggtc ggagatagat gatatcaaaa acattttgat ttacaattcc caaggtaagg
      541 gtataagact ttctgaagtt gctgacgtcc gagagggatt cgctccgcct tcaatagagc
      601 atctcgacag agagagagtt attaaagttt cgggttcaat ttacaatcgt cctttaggtg
      661 atattgcaaa ggatatgaag gctcaacttg cgaaaattaa ctatccacct cagattacag
      721 cagaaatttc tggatctgta gaagatcagc aggagtcatt caacgacttg tacactttac
      781 ttgcattggt tataatgctt gtttatatag taatggcagc tcaatttgaa tcttttagag
      841 atccatttat tatcatgttc tctcttccgt ttgcatttac aggagtattt cttgcacttt
      901 ggatgacagg tacatctcta agtattattg cacttatagg ttctgtaatg cttgttggaa
      961 tagtagttaa gaatggtata gtacttatcg actatattaa tctgaacaaa gagagaggag
     1021 catctgtcag aagggcagtt atcagcggtg gcagatctcg tttaagacct gttttaatga
     1081 ccacaacaac cacaattctt ggtatgatac ctatggcaat tggtatagga gaaggttctg
     1141 aaatatggca accaatggga atagccatca ttggaggttt gactatttct actattttaa
     1201 cccttattgt aataccatgt atctatactt cgttccattt tggtgatatg aagagaaaga
     1261 gaaagaaatt tgcggaagat ttatctataa ttgataatta atatgaaagc agtaatgata
     1321 gcgtacaatc aggctcacaa tgccgaagta caggaaattt tggacaaatg caatattaga
     1381 ggtttcacca agtgggaaga tgttcagggt cgtggcagca aggatggtga accacattat
     1441 tctactcatg catggccatc aaaaaatatg gtaacaatca caataataga ggataataaa
     1501 ttgtcggagc ttattaagca actcaagttc cttgatgata gagctgagaa acaagggtta
     1561 agaacttttg tttgggaagc acaacaggca tggtaatatt ttctttggat aagttattcc
     1621 aatgttataa attagcgtcg gtttaataaa aatttaatta tggcaatagc agtcaatgat
     1681 tctaacttca atcaaatagt tattaactca gacaaacccg tattggtaga tttttgggca
     1741 acatggtgtg gaccatgccg tatggtttca cctattgtcg acgaacttgc tgccgaatac
     1801 gagggaaagg cggttattac taaatgtgat gtagattcta gttctgatgt accattgaaa
     1861 tatgggattc gcaatattcc gactattctg tttttcaaaa atggtgaggt aaaggataaa
     1921 attgttggag caaccaccaa atcagcaata gttgctaaac taaatgcatt gatgtaaatt
     1981 ttacatgaaa aaggtatttg tgctgttgat aagtgtgctg ttatttagca cactgtctta
     2041 ttcgcagacc agatttggga ttaagggagg gcttaacttt acaaacatct ccaatctctc
     2101 ttcggatgta gagaaaactt ggaagaatca aaccggctat caattaggag tagctcttca
     2161 gattaaaatt cccgttattg gtttagccat tcagcctgag cttttgtact ctacagttaa
     2221 atctgcctct gacaatggaa atgagattaa tctagattat cttactcttc ctgtcaacat
     2281 ccaattagga actgacatgc tttttttcag gccatttata gttgcaagcc catttgtctc
     2341 ctatatgatt gacaataatg tcaggcttga ggatcagcct gtagatgaaa taaacaggtt
     2401 tgactatgga gtcggagtag gagtaggtat cgatatctgg aagctccaga ttatgggtaa
     2461 gtataattgg ggccttggaa agatagaaaa tgcaggagat gaatgggatc agtcagaaac
     2521 ttataaagac gcgactcttc agggatttca actttctatc gctttcctgt tctgattatt
     2581 aacatgcatt cgaaaagaag ttttttcaag tggccgcctc agcattactg aaggcggctt
     2641 atttgttgga ttataatgag tatagttaca tgtctctttg ttaaaaatat aaaatggaga
     2701 gtttcgatat tataagtgat atcggtgaag aatttcacag atacagggca cttatggagg
     2761 atagcctaaa cagcagaagt tccttgctga atgccattaa tacttatcaa ctccaaaaca
     2821 gcggcaagca attaagacca attttaggta ttctttcggc taaagcttgt ggaggtacag
     2881 taaatgacag gacaatttca tgcgtagcag tagctgaact gatacataca gcaacgctgc
     2941 ttcatgatga tgttgccgac aagtctgaca ggagaagagg tgtaaagact gttcaagcag
     3001 aatatactcc tgcagcttca gttttaacgg gtgattactg gctttcaaag gcaatgacta
     3061 ttttggtaaa tctttttgat cgccaaatta tggaatttta taccaatgcc gttgtcaact
     3121 tgtctgaagg ggagttgttt cagatgcaga aggcaagttc tctcgatacg agcaaatccg
     3181 actactttaa cattatttac agtaaaacag cgagcctttt tataggagct gtcaaaagtt
     3241 cagtggctac tgtttgtgat aaggaggagg tgctgatgaa tatggaaatg tttgcggaaa
     3301 atcttgggat agctttccaa atcagggatg acatttttga ttatatgccc tctttgaata
     3361 caggaaaacc cacaggcggg gatattgttg aaagaaagct cacattgccg ttgatttcgg
     3421 ctcttgattt agcagctgag gatgaaaaga tcagattgaa gcgtatgttg agagacagga
     3481 aaactaatga taatagactt gttcaggagg catttagaat tgttgagaaa tactcgggaa
     3541 tctatttagc tcaacaggaa ctgttaaaat actgtcagag agcagaaaaa gcactttctt
     3601 ttcttgagga atcttcttat aaaagagatc ttgtaacgct tgccaaatat gtcggggaaa
     3661 ggctttacta aaataatctc aaagctgctt cttcaagttg cttataaaaa tctttgccaa
     3721 atgcaaaaat aagagggtct cttaaaaact tatagacagg gattccttct cttttccctt
     3781 taacgaaggc atccatacaa aggtaccact catgtagatt taaggcagtc ataccattgg
     3841 acagttttga cactcttata ggataaagcc agcaggatat gggttttttc atggagcagc
     3901 attgtgcatc aaaagctttt tcaataccac acaaacagtt gttgtctttg tcaaaccaac
     3961 tgtacgcaca ctcttctcca tttaccaatg gggtaacagc atctccgtca gcatcaataa
     4021 caaagggacc ctgctcctta acagagcgga tcccttccac agtcaggtat tttgaaaatt
     4081 gttgaaattc tccggcaaga atactacact cgttcttttc aagtggagct ccactatcgc
     4141 caattataca acaagctccg tggcatttgg aataatcaca gcaaaaatat tctgttatga
     4201 tttttggtga aacaagtata ttctcaatag caataatcgg agaataattc atactatttc
     4261 ttaaaaccat tcattattat ctcgacttca tttccttgtg taagtgcagt cttgatgaag
     4321 ttatcaatct tctcaggagt catgcttttg agagttgcct caaaagttga gtgcatatcc
     4381 ttgcctaaga aatagcggtt tgtaagaaca gagagccaat aattattttc tctgagattt
     4441 tgagtataat ttttctgcat atactcaatt accttggcaa aatctgtctt ttcgacgccg
     4501 ttcttcataa cattttctat ctctttctgt gctcttgcaa ttagtctgtc tttgatggct
     4561 acgtctgtat caaagccata gagaaacaca aagtaatctt cgggataata tgtaacactc
     4621 atgcttacac caacgccata agtaccacct tcatcttccc taatggtcct ggtaaatacc
     4681 tgatcgaaga tttgtttggt aatgtctgcc ataattttgt tttcaacagt atatggtatc
     4741 ttgcctgagt agatcttgta gacagttgat ttaggagtct gcatctcttt gtcaaattgt
     4801 tttgaaatga ctcccctaac agggacattg ttcaggttct tccagtcttc ttttttggcc
     4861 ttgtttgcag gcaaagatcc aatgtatgtt tcaaccagtg gtttaactgt ggcaggatcg
     4921 atgttaccaa caaatacaaa ggtaaaatca gcggcatttg caaaacgctc tttgataatc
     4981 tgtaaggctc tgtcataatt aatctggtct aacatctttg atgttaacct gtttacgtat
     5041 gggttgttat tgaagagagt cttggagaga gaatcttgga aagcaatcat aggaagagat
     5101 tccatattaa ccaactgagc tctcattcgt gtaataaatg aatcgaaagc ttcatcatct
     5161 tttctgacgt cggtatatga aagataaatt aattgcatga atgtttctag atcctttgga
     5221 gaagaagatc cactcagctg ttcgttattt attccaatag aagggttgat agatacattt
     5281 ttacctgcaa gattcttctt taaatctatt gcactgaatt ttccaagtcc tcctaaagtt
     5341 gctatatcat caaggacttt tgtaattact acatcttcgg gattagtcag tgaaaatcct
     5401 ccacggcttg tggcagtgaa caaaatctga tcttctttga aatcagtttt tttgattacc
     5461 acagtagctc cattggaaag tgtccaaaca agggcatcag tgatcggttc gcgggtttcc
     5521 ttaacaactt ttcctgcttt tggagttaca gcaacaagag gctcattgga aacagtctct
     5581 gtatatggtt caagcttgtc attgagagca ctttcataaa gagagagaat ttcttcttta
     5641 gttggcactt ttatcccttc tttctgaggc atcattacca gcattacaac attttctctt
     5701 gtaggtagtg attttgcata tgtatttatc tgatcaacag taatagcagg tgctatctgc
     5761 tgcatcaaag tatactccat ctcaattcct ggcaaagctt ctccagtaag aaaatggttg
     5821 actaaatttt gaacatagta atcattcttc tgattttcac gctctttata aagtctctcc
     5881 agcctgctca tataatttgc ttttgctctc tcatactctg aagcagtaaa accaaactgt
     5941 ctcatccttt cagcctcgtt aataattgtt cttgcacccc tttccaattc tccttcacga
     6001 gcttcaacac ttagtgtgaa agcctttttg gtctgagaaa cgaagaaatc tccataactt
     6061 gcatctgctc caacaaaagg aggatcggat ttttgaacta actctgaaag gcgagaattg
     6121 agcattgagc agaccatatt gttcatgtag tcaatgactg tttgtgcaac tgttggccgc
     6181 agttcaaccg gcactacatc atgtttatac attagataaa actcggttga ttttgattcc
     6241 gggtcacttg ctattgcaac aattggttcc ttgttgtctt ctacagggaa ttgaattctt
     6301 tctgccggat tttccttttt agggatagaa ccgaagagtg atttaatttt attttcaact
     6361 gccacagggt cgatgtctcc cacaactatt attccttgaa ggtctggacg ataccacttg
     6421 ttgtagtaat cacggatttc ctgataagtg aaattattga ttacatcaag aagtcctatt
     6481 ggcagtcttt caccatatct attgtcgggg tagatctcgg gaaggacttt ctcgagcatt
     6541 ctcatagaag cattgctgct tgttctgagc tcttcacgaa taacacccct ttcgtcgtca
     6601 atgtctttgt ccgtaagagc tattccacat gaccagtcgt gcaaaattaa aagacaggaa
     6661 tcaataatgc catctctttt aagaggaacg ttggcaatat gataaactgt ctggtcgatt
     6721 gaagtgtagg cattgagatt tgcgccaaac ttaacaccta ttttttcaag gtaagaaatg
     6781 atgctgtttc cggggaaatt tttcgaacca ttgaaggcca tatgttccaa aaaatgggca
     6841 agacctctct gattttcttc ttcgaggatt gatccgactt tttgaactat gtagaaatct
     6901 gcctggcctt taggttcgga gtttttgaca acataataag tcagtccgtt gtcaagttta
     6961 cctgttttga ccttagggtc caggggcagc gtttgaggca tttgtgccga agccagaaat
     7021 gccattacca aagttaccgc aagggtaaaa atctttttca tgaaagttaa ttatttttaa
     7081 tacagtataa tatagacaaa tatagatatt attttgtatc atttattctt tttattattt
     7141 ttgtaaggat agcgattttc tgttttatct aaataaatgc gtaatatgaa aaaaattgtg
     7201 accattttat cgacttgcat gctctctctc ttctctttta cttttgttta tggacaagac
     7261 atagctgctg caattgaaac ttacaatgca ggagcgactg ctcttgcgtc aaataactat
     7321 gttgcagcca ttgagtcgtt taataaggcg attaagatgt ttgaagcaat cagtgaactt
     7381 gacgaagatg gggcatctgc atttaaacag tgtaaggaaa tacttcctca gattcatttg
     7441 agatatggca aagagcttgc agtaaataaa aattttgaca atgctttggc acagttggat
     7501 aaggctatag aaactgctaa gaaatacaat aatccagatg ttgagaaaga ggctaatgaa
     7561 ctcattccgc aacttgtcat ggccggtgca gacgacaact tgaatcttgg tcttttcaat
     7621 gaggcgattg ccgggtataa taaaatgttg acttatactc caaacgatcc tgatgtatat
     7681 ttgaggttgg gtatagcaca gtccaaacta aataatgaag atgcggcaat tgctgcctat
     7741 ttaaaggcaa tcgaactggg cgacaagcaa gacgcaccaa aaatgctttc aaatatttat
     7801 ctcaaaaaag ctgcggctgc tttttctgac aaaaacaatt cagaggctct tgctatggca
     7861 aaaaaggcaa atcagtatta tgagacagtt caggggaatc agattattgg tttaagtgct
     7921 atgcaactta agaaatacga tgacgcgatc cctgcccttg aagcatatat tgctcaaagc
     7981 aatgatagta aaaataagaa tgcgattata tacaatatcg cagcggctta cgaggctaaa
     8041 ggtaatattg ccaaggcctg tggctactac aaacaaattg tttcagatcc aaaatacaaa
     8101 gctgccgtag aatataagat taatacagtt cttaagtgca agtaactaca ttggagcttc
     8161 ttgcacctgc aggaaacaaa gacatcggca ttgcagcaat agactgtggt gccgatgctc
     8221 tctatattgc aggctcaaag gtctatttaa ctcttaatac cattattttt gacaatgagt
     8281 taggaaaggg tttgactgca gaaactgtga aatgattatt tttggttgat tttttcaaat
     8341 aggcaatgaa attttctgat atagtcggac aaaatagctt gaagcagcaa ctcataaaac
     8401 tctttaagga agggagaatg agtcatgcaa tgcttttctg tgaagaacag ggctatggag
     8461 cgctttcaac agctcttgct tttgttcagt ataatttatg tctcaacaag agtgcggaag
     8521 actcatgtgg cacatgtcca acgtgcaata agatacaaaa gatgattcat ccggatctcc
     8581 actttgctgt tcctgtcaat tccacaaaaa aaattacagc tgataaaaaa cccgtgactg
     8641 atcttttttt agatgattgg agaagagcaa tatctgaaaa tccatatatt actgagcagg
     8701 aatggtatga tttgattgga atagaaaaca aacagggaat aattggggtc aatgaagctg
     8761 ctcaaatttt gaaaaagttg agttttcgtt cgtttgaagg aggtgacaaa tatgtaatta
     8821 tttggttacc tgaaagaatg aaccaggaag cagccaataa gcttcttaaa ttaattgaag
     8881 aacctcctgc cggcacatat ttttttatga tttctcactc tccggagaga attatcccca
     8941 ctattgtgtc tagatgccag attattagct tgatgcctat agagagagag atgctggctc
     9001 aaagattggc ggaagagttc aaaatatctg aagaagaggc tttcttttgg tcaaggatat
     9061 caggaggtag cttgagcaag gccagagaga taattaattc agacagagag actgttgaag
     9121 atcacgagat tgtggtaaaa ctactttcag cctgctgtag taaagacatg cctgaagtta
     9181 tttccctttg ggaaagactg tcagcgatga atagagagaa acaaaaaagt ttctgtcatt
     9241 atgctcttga atttttaaga gagttgtaca tgacaagcct tggtttgttt gatatttcaa
     9301 atactcctgt cagaagaaga gaaaatgtcg ttagtttgtc gaagagaatc aacccttctt
     9361 tttatatcaa agcttatgag attctgaacg atgctttaca ggacatagaa agaaatgtta
     9421 atgcaaaata tatttttgca gacatgagta atcgcttttt tttatctttg tagctattat
     9481 gaaagatcag aaaattataa atgattgttc cagaggatgt aagatttgga aggattccga
     9541 cgatacttta aatgtgaagt acgacaataa ctgttgcaag ctgagtgtct ttgattggtt
     9601 acaaggaatt tcggatgaaa agtcaaagga tatatttgaa gtgcgtttca aaaataccag
     9661 aaaatcttat ttcaggaacg catcagatca gcaaattgat gtaggtgata tagttacagt
     9721 ggagacaacg aatggccatg atgtaggcat tgttactctt gcaggccccg tggtgctgat
     9781 acaactgaag cgcaacaaca ttgatattga tcactttgaa ttcaaaaaaa tatacaggaa
     9841 atcgaaagga gttgatattg aaagatggca ggatgctata tcgagggagc ataaaacaat
     9901 gattagagcg cggcagattg ctgcttcact gggcttgaac atgaagatag gagatgttga
     9961 atttcaaggt gatggtacta aagctatttt ttattatata gcagatgaaa gggtagattt
    10021 ccggcagttg ataaagttat ttgcagagga gtttaaaatc aggattgaga tgcgtcagat
    10081 aggtgccaga caggaggctg gacatattgg gggaataggt gtttgtggcc aggaactctg
    10141 ctgttcaaga tttattaatg attttaaatc gattaccaca caagctgcca gatgtcagga
    10201 tctctcactt aatccgcaaa aacttgccgg acagtgcagt aaacttaagt gttgcataaa
    10261 ttacgaggcg gcagtttaca tcgatgctca ttcatcaatg ccaattgtta aagctcctct
    10321 cgaaacagaa ggaggcccgc tttatttgat gaaaagtgat gttttgaagg ggattatgtg
    10381 gttttcatat gaggagagca gcatttcaga catgtttcct cttacttttg agtgtgtcag
    10441 agaagttttg cggcaaaaca caagaggcat caaagtcccg gatcttacgc cagacagtga
    10501 ttccggtcct gaatttaaaa gtgctgttgg tgagggaagt atttcgaggt ttgatgaaga
    10561 aagaaaggcg aatcatttaa atcgtaaaaa aaggaataaa caatcttctg gaagaaataa
    10621 tgaaaatcag aagtaaggta ttactgttgt ttttttttgg agctttgttg tgttcatgtg
    10681 caaaagatga ttatgagcct tcggtgcaat ggttcaagtt taattgcagg aatactcaga
    10741 gatcgtttaa tgttgatttt tcattaagga tttcggtaac atataaagat gaaacgcttc
    10801 cagttatgct tgaaattctt tctccttcag gcattagata tagagacact ttgaacattc
    10861 ctgttaaaaa gcaggttagt gacggcttaa ctaccgttgt aagaagcgga gtctggagag
    10921 atgtactctg gcactatcga aagaacgtca gttttcctct aacaggcaga tggttattta
    10981 ctatatatta tttagaacct ggcacaaaaa aggggaaaat taaagagttc aagttgaaga
    11041 tacagtaata atttatatgg ggaaggataa actgaaaaga tttgccgaga ataagacttt
    11101 tagtaatctt tatcaaccag atttcagcga agtcttcaga aaagattata ggcttaaagg
    11161 gaaatggaga cgggaagttt ttgggaatga caaccctata gttttagagc ttggatgcgg
    11221 cagaggagaa tatactgttg aaatggcaag aagaaatccc tgtaataatt atatcggtat
    11281 tgatattaag ggagcaagat tgtggagggg tgcaaaaagc gcacttgaag agggccttgt
    11341 taatgttgtt tttgtcaggt gcagaattga gtttgttgaa agtctttttg gtgagaatga
    11401 aatatcgtct gtatggataa cgtttgcgga tcctcaaatt aaaagggaga ataaaagact
    11461 tacctcacca ttttttctag acaaatatgc caaatttctt ataaatggtg gcactgttca
    11521 tctcaaaacc gacagcctgt ttttgcatga atatactctt ggaatagtta aagaaagggg
    11581 gtatgaaatc attgagagta acccggatat ttacggtacg ggatacgccg gacaagataa
    11641 tatgctagat atcaaaactc attatgagaa aatttttctt tcccagggac ttccaataac
    11701 ttatatgtct tttaagattt ctcgggattc ccttgggagg cgtactcaat agattttgcc
    11761 aggcgataaa catcctttct catccctttc aggacaatag gattatcgct gaaatcaacc
    11821 actattgtgt cttcaaacat tctgaacatt ctagttactt tggatttgtg tctgaaagta
    11881 atagtggttg catcttctgc tacttttata taattgctgt cttcaaacac tttgagtatt
    11941 ttatctctct cttcagcaaa agagtgattg aggtaggcac gtttattgat gaaacttacc
    12001 agaggatatg ccagagaaat tacaacaaag aacactaaca attggacgcc tgttccaggt
    12061 ctgaatagtg cagaatagct tagttcctgt cttgatgcca gagcaaaaat tgctataata
    12121 agggagaaaa taataacgaa ataaataaga tatttaacaa ctctgataaa atacttttta
    12181 aaatcaaatc cgcttttcat agtagaatat gtgttttata attaaggaac ttatgtacca
    12241 taaatacaaa gctaaataaa aatcttaaat ttgtttttat attacaaaaa ttaataaagt
    12301 tagaattatg gaaaaagaga gaacacttag aaatattgta atagctctgt cagtaattgc
    12361 acttctgctt ataggagttc ttgcatacat atggatagac agaagcagga taataggaga
    12421 tctgacagtt gaaaaggagc agttaactca acagacagag cagctgaagg aagaatatgc
    12481 catgttgaca acaaataatg acagtctcaa tgtgcagctt tcaaaagaga gggaaaaggt
    12541 cgagcagctt attgacaggc taaaaaggac agaggcaacc aacagaacca aaatacgtca
    12601 atacgagcaa gagcttggaa cattaagaag cattatgagg ggttatatta tccaaattga
    12661 ttcattgaat accttgaata tcagtttaag gaaggaagct gctgctgcaa aggatgaagc
    12721 aaaagagaca agacagaaat atgaagaact ccagactaca accaacgaat atgctctcaa
    12781 gatacaaaaa ggttcaatgt taaagggtag agggatcaat gtcgttgcaa ttacggcttc
    12841 caacaaggag actgaccgca gcagccgtac agttaagatt aaaacgtgct tgtatcttgt
    12901 agaaaacaat attgctacaa aaggccccag aaagatatat atcagagtga aaggacctga
    12961 tggtattttg atgactgacg gaagtcagca gatattcaac gttggtggag agcagatgat
    13021 ttattctgca gtaagggaag ttgattacca gggatccgaa cttgaagttt gcatctattt
    13081 tgcgggcagt gcaccttttg tcaaaggagt ttataatgtc gatgtttata ctgaggaagg
    13141 caagctgggt acagctgacc ttttacttaa atagtcaaat tggcaggtat ttacatacat
    13201 attccttttt gctcgcaatt ttgtatctat tgtgactttt attctataag aaacaagaag
    13261 gacagagttg aaaaatatat agcatcgctt ctatgtgaga tagaacagag aagcgatttt
    13321 tttaaaaact gcaagataaa gccgacgact atttatatag gaggggggac accctcgctc
    13381 ttgtcatctg ataacctgtc aaaaattgta aagtctgttt atgcacaatt tggaattgac
    13441 actttttctc aaattcaaga atttactatt gaggttaatc ctgatgacct gaccaaagat
    13501 tatcttattc aactaaaaga tattggtata aatagactga gtattggtat ccaatctttt
    13561 tttgatgagg atttgctttg gatgaatcga cgtcatgatt cgtgtaaggc aatagatgct
    13621 tttttcatgg caagagattg tggctttact aatataagta ttgatctgat gttcggcttt
    13681 agtctattga cagaacaaaa actcattgca aatctcgaac ggttgatttc aataagaccg
    13741 gaacatttgt cattatacca gcttggcatt gagaaaggga ctcctctcgg gaaaagttat
    13801 gaatgtggag aatatgtccc tttgtcagat gataactgtt cattacagta ttctcttatt
    13861 cagaatatgc tctcaaaagc gggttatatt caatatgaaa tttcaaattt ttcacttcct
    13921 ggaaaagaag ccttgcacaa tagttcttat tgggagcata aaccttatat tggatttggc
    13981 ccgtcagcac attcgtttga tggaaagata agaagctgga actgttcaag tctgagtaaa
    14041 tactgttctt ttttacaagt aggagacagg tgttttaaat gtgaaaattt atctcccagg
    14101 aatatattta atgagaaaat aatgctttct ttgagaagaa cagtcggagc agacctacaa
    14161 gtcctcagaa acgggacatc tccagagatt tattctttat ttcaacaaca attagaaaat
    14221 gcattaaaga gaggggaagt aattatggat ggaaataaaa taaaaatccc accggacagg
    14281 ttctttgtat ctgatgggat aataagagat cttatggtag tttaaaactt ttaattttca
    14341 gcatatccct tttgacctac aataggctgt tatggcaata gtggcgaagc caagaaaaat
    14401 aaaagtagca tagatataag caataacctg ccacctttta agccattttg aattgttcat
    14461 acccaaggtt tggaaagcac tccagaaacc atgtgtcaga tgaaaccata tagcaaagaa
    14521 ccaaataaga taaagtataa caatccatat attgctgaat gtaagttgca tgagattgtt
    14581 tacattttcc ggttctctgc ccgtaaaatc cttcaactgc atatgtgccc aaaaatgagt
    14641 caaatggaat ataaggaagc ccaaaatgat aactccgagc acaaacatgt ttttggaagc
    14701 ccatgaatct gatgcgcctt tgtgtgcgac tgcatatctc tcttttcccc gcgccatcag
    14761 gtttgtgata gtgagatagg aagcgtagaa aatatgaatt ataaaaccgg cagcaagtac
    14821 tggaactatt gcaactacaa cgggcgaact cataattatt ataatggtgt caaaagcctc
    14881 aggtccaaac aggtatgcag aatttgcaaa aagatgaacc aaaaggaaaa gcatcagaaa
    14941 gagtccagta atactcataa taagtttctt accgatggaa gacgtaaata tgttgctcat
    15001 gccgctataa ttttattatt aatagtatct ttttttgttt ggcaaagata tagccttgct
    15061 tgttaatttc ataatattta gctacttttg ttctattcag atgattgaat gacataattt
    15121 aatacctaat aattaatgaa atacaaaaga gttctgttga aactgagtgg agagagcatt
    15181 ggaggcagga ttggtgttgg tttagacgaa aatattctat ctcaatacgc cagggagata
    15241 catgatacag tagctttggg tgttgaaata gcaattgtta tagggggagg aaatatattc
    15301 cgaggacttt caggggtcgg aaagggcttt gacagagtca agggagatca aatgggaatg
    15361 ttggcaacaa tcattaacag tattgcactc tcgatggcac ttaagtccca aggaactcct
    15421 gcagaggttt ttacctcaac tcctgcagaa ccttacggga tctattacgg cagagataaa
    15481 gccgtaaagt ttatggagag cggtggtgta tcaattattg ctggtgggac aggtaatcct
    15541 tttttcacaa cagatacggc ttctgctctc agagcagctg aaattggagc tgaggcgctt
    15601 cttaagggta caagagtaga tggtgtttat tcggctgacc cggagaaatt tccggatgct
    15661 ttcaagtata tttctatttc atttgacaaa gctcttgaag agaatcttaa aattatggac
    15721 ttaactgcgt ttaccttatg caaggagaac agcatcccaa tagtggtctt tgatgttaat
    15781 ataccaggga accttgttaa ggttgttaat ggagaaaaca taggtacact tatttcttat
    15841 gataattaat ttattgtttt atggatataa ataatcaaaa agaggttata gcagcagcta
    15901 aagaaaaaat gatcaaagca atttttcatt tggaagaaga gcttaaatct tacagggccg
    15961 gaaaagccaa tcctgcaatt ttctctaata ttttggtaga ttattatggt accatgacac
    16021 ctattcccca agttgcaagt attactgctc ctgatgcaaa aaccatgttt atccagccat
    16081 gggacaaaaa gatgattcct ttgattgaga aggcaataat ggctgccaat ataggtttta
    16141 ctcctcaaaa caatggagaa catataagaa taaatgttcc tccccttaca gaggagaggc
    16201 gcagagagct tgttaaaaag gtgaggcaag aaggcgagaa tgctaagata agcattcgta
    16261 atgctcgaag agatggtgtt gagttgctta agaaatatca gaaggatggc cttcctgaag
    16321 atgttgttaa ggatggagaa agcattcttc aaaaggagac tgaaaatttc aacaggagag
    16381 ttgacgagat tatactcgca aaagagaaag agattatgac agtatagaga tgtcggcaaa
    16441 aattggcagg gtaaggttct accttttttt cggtattttg atattatctt tttgggtaat
    16501 atcctgtaaa gctccccaaa ttgaaggacc gcaggagtct tatttacctt ctgaaattgc
    16561 accttcattg tcagaactgc ctcttcgcat agaacttgat gtcaaaaaaa ttgagagtgt
    16621 ggttaatgca agcatgaatg gtcttcttta tcagggagac aagcttgcag gtgaagacct
    16681 ttctgtcaag atatggaaag gaggtcaatt tatgtttttt gtcaacaaca atgaaattac
    16741 ttataaggtt ccattgaaag tatggtgcag atatggatgg aaagttgaga aatttggcgt
    16801 ttcgttttca gatcattatg aggcaacagg gacaatctca ctggtataca aaacagcggt
    16861 gggtattgat gagaattgga aacttgttac aaaaacaact cctgcaggat acgaatggct
    16921 tgaaaagccc aaagttaaag cgataggagt tagtatcccg gttacgccaa ttgcaaatat
    16981 agcactttca tattttcaaa aaactataac agagcaagtt gataaaattc tcttcgagaa
    17041 cgttccattg gacaaatatg taggagccgt atgggatgag gtacaacaac catatttact
    17101 gagtgatgaa gataatctct ggatcagaat aactccaaag gaaatactgc ttgctccgtt
    17161 tacaaccaaa ggcaacaaac tgaatattac tcttgtattt tatgctcaag ttgagtcagt
    17221 ctttggtgct cagcctgttg taaaaaacaa agtgccgctt ccttcgctta gatatattga
    17281 acgacagcca cagcaatttt caattaatgt gggggctgat gtaacttttg ataaaattgc
    17341 ggaatttgcg cgcattcaat tgcagggaaa aagttttaca gaaagtggta agattgttaa
    17401 gatcgataat atttcacttt atagtagcaa tggtagagct gtggtagtag cagatctgag
    17461 tggttcatac aagggaagaa tatattttac aggtaagccg gcatataaca gcgaaaaaca
    17521 atctcttgaa atcatagaac cagaatttga cattaagacc cagaatgctc ttataaagtc
    17581 tgcaaactgg ttattacatg gcatgatact caaaaaaata actcccatgt tgacttatcc
    17641 tttagaggag actctgaaaa cattgaaaga tcaggctaat tccatgctta aaagttatga
    17701 ggtgtatagt ggtgtacata taaccgggtc gcttgattct ttggctgtca caacagtcag
    17761 tatggtgcct ggagctgtaa gagtaggagc ggaaattaag ggaaatgtgg cactgataat
    17821 agatgaactt aagttctaat tgtttttggg ttctatgata gaaagatccc aagtatggac
    17881 ttgcttgtta aacttactct tttttctacc tctatttccg tcgaattgac caacgtattt
    17941 tattttaact tgatctttta tatttggagc gagtaatgta tctgccattc catgagtgag
    18001 aataacacat ggcatagcag atactacagc agcagaatcc ttgatattta gtggttttat
    18061 aattctgtgg gcatcataca cttgttctat tctcaactcc tttgacgaat catagtaaaa
    18121 ttgaagattt gctacttcag gcatatttct cgtcctgtcc agtccgttaa attccggatt
    18181 gttaaaggct cctgcaatta acggcattaa agaaacactt acaagaacaa acagcgctgc
    18241 aattccgcca aggaacttca tagggtctgg ttttacaata gattttttta tcatatagat
    18301 gccgattgtt aaagaaacta cagttataaa agaaaagttt aaaattgaaa gtttaacatc
    18361 atgaaatgga gctccttcat tgagaataag cagatatatg ccaataggga ttataaatgg
    18421 cattatagca ataggtagag tatttaaata gtaaatccat ttcttgtaat tcaatttttc
    18481 tttgactgcg gatgcccaat agtcgaaaat gtatcccatt gtcatagctg caggaatcag
    18541 catcggcagc aggtatcttg ttttcttttc aggcaggatg gaaagacaaa ctattacaag
    18601 aatcatccag agaagtgcaa aagaataacc tttgggatga ttcacttttt ttctccaata
    18661 accccagaag agggatgtga tacaaagtag cgtccatata cctgtctcaa atgcaaaatc
    18721 ataatagtag taccatggtc ttgtatgata attggtccag ttttgagatt ctgtgccaac
    18781 aatcttaaga aattgctcag gatgtgatag atacacataa agataccacc agccacccac
    18841 aattatcgct gacaaaacaa aaatggctat acctttccat tttcccctaa gactattttt
    18901 atatattaca aaataggata ttagaaatgg gagaaacaaa ccgtataaac taataggccc
    18961 tttgctcatg aatgaaagcc ctgtcagaaa gcctgaaata gcaaacagac cggtgcctcc
    19021 ttcatttttc cgtacagctt tgatcaaaaa caaaatagca cctgtcataa aggcatggca
    19081 atatatatcc cagctagccg ttctccccgc cataattata ttgtaggatg tacacaataa
    19141 aatagtggat atcaatccat atcttttatt ggaggttagt tcaaccgcaa ttaaatagag
    19201 catcaaaacg agaaatgtgg cagcaagtgc agacattgag cgctgaactg caatattatc
    19261 aggtgcgatc atttcagcaa ctgcagccag ccacgtaggg agaggaggtt tctggagtct
    19321 tatctctcca ttcattgtgg gtaccatcca atggttgtcg tataccatct ctcttgccgt
    19381 aataatgttt ctcgactcca tgatttcagt aaagaggaag ccgttattta caaagaatgt
    19441 aaataggcaa agagaggcaa ttattaaata ggaggtatat tttttcatat ttctaatcac
    19501 tcaggatttt gtgctattta taatttggaa agtgaatttt ttctgtaaaa aataactaac
    19561 aaatacagtt ataattgaaa caatcatatt ggaaatagac gggaaaatat gtaaaagatc
    19621 aacaaaaaac ttaaggccaa aataatttat taagatatta attccataca caataagata
    19681 tctgaatagt tgtcttcttc ccttgagatt ggattctgta aatgtcacat acttttgaag
    19741 aagaaatccg gaaaggagta ctatgggaaa ttttatgaca aaagcagcta tatgagagct
    19801 tagtgtaaca ataccaagat gtagcatttt tttttgcaaa acgaaattgt aaatgacaaa
    19861 atacaaaatc cagtcaaaaa caagattgac aactccggta actccatatc tgaaaaattg
    19921 gataggcata gtttttctaa aaagaggata gaaatgatcg ataattttgc atatccatct
    19981 tcctatcctc tcaaaaagaa tctctaagct atattttatc tccatctttt actgagcaga
    20041 aaaattcaga ttgccaaaga tagaggattt tttttctaaa ataattccta taatgtaatc
    20101 tttacaggtc taatcatgtt ggctggatca aggattttgt caagttgttc ttttgtaagc
    20161 aattgtttgt ccagaacaag ctgataaaca ctgcaaccac tttccagtgc ctccttggct
    20221 atttctgtgc tgttggtata tcctataaaa ggtttaaggg ccgttacaat ccctacggag
    20281 tgctcaacaa gataacggca gtggtctgca ttggcggtaa taccgtctat gcattccact
    20341 ctcagagtgt caaatgctct ttttagccaa agagaggatt ctacaaggct gtgaaccaaa
    20401 actggttcca tcacgttgag ttcaagctgg gctgcctctg aggccattgt aatagtgaga
    20461 tcgttgccaa taacgcggaa gcaaacctga tttacaactt cagggataac agaattgact
    20521 tttccaggca taattgaaga gccaggttgc tttggaggaa gattaatttc agcgagaccg
    20581 gttctgggac ctgaagacat aagtcttaaa tcgttgcaga tttttgaaag tcttattgca
    20641 atcattttca gggctgatga atatgaaacc atcggcgagg tatcatgtgt cgcttcaata
    20701 agattgtcgg caagttttat tttccatccg gtgatttctt ttagaaattg cgtacaggcc
    20761 tcggcatagc ctggttctgc agtaatacct gtgccaatag ccgttgcacc catattaata
    20821 attaaaagct ctttggcagc attttcaaga tgttttagct cgtgcctcat agctgatgca
    20881 aaagcgccga aactttgccc gagtgtcatg ggaaccgcat cttgaagttg tgttcttccc
    20941 attttgatta ctttggcaaa ctctgtctcc ttcacggcaa atgagtctat caatttctcg
    21001 agagctgctt taacatctaa atgtaccatg taaaggccaa ggtgcatggc tgtcggatat
    21061 gcatcattag tagactgggc catgttaaca tgatcatttg gatgacagta ttgatagtca
    21121 cccttttctt ttcccattat ctcaagagct ctgttggcta ttacttcatt agcattcatg
    21181 ttcatgcttg ttcctgcacc tccctgaatc atgtcaacag gaaattgttc tgtatgtttt
    21241 ccgtcaataa gctcctgaca ggcagccaca atagcatcct ttagagaagt atcgattagg
    21301 cctaatctgt ggtttgcaag cacagctgcc atctttacca tcccgaatgc cttaataaat
    21361 tgtggatagt ccgatagtaa ctgtctgctg atgtcgaaat tctctataca tctaagcgtt
    21421 tgtacaccat acaatgcctc tgcaggtacc tctttatctc ccagaaggtc gtgttcaata
    21481 cgttttgtac cttctgactt aatatgatat ttcttcattt tggttaataa ttatattaaa
    21541 tatcttcaca aaggtaacca tttattgtta aatcgaaacc ccgacggtta atgttgtaat
    21601 aaattgtaat aaaaaaagag gattatcctt tatgatatcc tctgtactta aaaatacagt
    21661 ataaagctct gaatccgtcc ttccatccaa tttttttacc ttcacggtat gttctgccat
    21721 aataggagat acctacttcg tagatacgga ttcccggaat tctggcaagt ttgcctgtaa
    21781 cttctggttc gaacccaaat cggttttctt ttagtttcaa agactgaagt attttggtgt
    21841 caacaagttt ataacaagtt tccatatcag tcaagttaag attggtgagc atattagaca
    21901 taaatgtcaa aaatttgttc cctattgtat gccaaaagaa aagcactcta tgaggattgc
    21961 ctcccataaa tcttgaacca tatactacat cggctactcc tttagccaca ggtttgagta
    22021 aatcattgta ttcttgtggg tcgtattcca gatcagcatc ctggataatt gtatattctc
    22081 ctgaggccaa agcaatgcct gtatgcaatg ccgctccctt ccccatgttt tttgtatgtc
    22141 ggtggtatcg aatatccatt tcgggattta acttcatgta aagtttcaca gcttcttctg
    22201 tattatcagt agagcagtca tttatgatga ttatttcttt atttatgtta ttgattaagg
    22261 atacatcttt aattttgtca agtatcaaat gaatggttgt tccttcatta taggcaggta
    22321 tcactatcga tagtttattg atcattttgt cacttgttta ctttttaata attccgcaaa
    22381 tatcattgta aaaggatcat tagggtaatc tctctgcatc atctctgtct ctctctttaa
    22441 tttatcccaa tcttgaagtt ttaaatagat ttgcatcaaa tctactcttg cactgtaata
    22501 gtccggaact attttcagtg agttaagcaa agcattttca gc
//
LOCUS       DAOTQL010000014        20917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2542_length_20917_cov_4.951874, whole genome shotgun sequence.
ACCESSION   DAOTQL010000014 DAOTQL010000000
VERSION     DAOTQL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20917
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_20917_cov_4.951874"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="P5293_01740"
     CDS             <1..422
                     /locus_tag="P5293_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRR48688.1"
                     /translation="AFFGEKDYQQLAIVKYFTRQLGYPVQIIACPIVREDDGLAMSSR
                     NTLLTPEQRKVAPLIYQALLNAAALAKEEKITPEELIKITINKINNSPQLEVEYVEIV
                     DSATLQPVSLWDEAEEIQMWTAVFAGKTRLIDNIKLK"
     gene            424..777
                     /locus_tag="P5293_01745"
     CDS             424..777
                     /locus_tag="P5293_01745"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRR48689.1"
                     /translation="MQLEIMKSKIHRARVTEANLNYIGSITIDADLADAAGLYPNEKV
                     SVVNINNGERIETYVIMGERGSGKIGINGAAAHKFAIDDLVIIMAYATMSPQEAIAFK
                     PKIIFPDSKSNKLIP"
     gene            794..1789
                     /locus_tag="P5293_01750"
     CDS             794..1789
                     /locus_tag="P5293_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRR48690.1"
                     /translation="MKKFYSSLKYLLLLSLAGILLWFSFKGVNWLDFLNGIKDANYWW
                     ILASMTISILAFFIRAVRGRMIMQTLGKPVKLRDSWDGVNIGYLTNFAAPRAGELARC
                     GVIAKKSGIPVESVVGSVVLERSIDLLSLIILVILVVFLSWEQFGTFINKEILGALPG
                     KFSSNLILVASVIILAFAIFIYIVFKYRERHPIFKKIVKITKGLFDGLVSGFRMKQKW
                     LFILLTILLWTCYWLMSLTTIYAFPKVGFLGATDALFLMVVGSLGWLIPVQGGIGAFH
                     FVTTLALAQVYGISQTTGIVFATISHESQALTMIICGTLSLVSFSLSKRKKEVVK"
     gene            1786..>2645
                     /locus_tag="P5293_01755"
     CDS             1786..>2645
                     /locus_tag="P5293_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HRR48691.1"
                     /translation="MMKKILLTLFLIFPITVFAEHQKNNGSDIYLIISSYNPDTQRMS
                     DFITDFENIVTERGEKADIVIEDLGCKSFSEESFMWKNQMKNTLNKFRKSNLKAIILL
                     GQEAWASFLSQDSIPKGVPFFSSFASVNGIELPKDSVGHEWLPESVNTVEKAKKLGHG
                     GGYLNHYDVTANIKLIKKLYPKTDNIIFVSDNTYGGVSLQALVRKEMKQFPEIKLTLL
                     DGRKFTIQEAQQIMRRLPQNSAILMGTWRAKKDGIGLTNTSLLKLRQANENVPIFTIS
                     GTGFGLVAVGG"
     assembly_gap    2646..2745
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2746..3462
                     /locus_tag="P5293_01760"
     CDS             <2746..3462
                     /locus_tag="P5293_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR48692.1"
                     /translation="ELIEAKERAEQADRLKSAFLANMSHEIRTPLNAIIGFSNLLCSE
                     DPSPEERSKYNSLIAKNSDILLTLINDVLDISRLETDQMHFTYQQADLLSICNHVINS
                     IGHQKKPEIDYVIDPECKSYIVETDTQRLIQVLLNLLTNANKFTEKGSITLKYVVDEK
                     LEVVTFSVTDTGCGIPRNKFDSVFERFGKLNEFSQGTGLGLAICTQIVKKFGGKIWID
                     PEYVSGARFCFTIPIKHNNT"
     gene            3482..4222
                     /locus_tag="P5293_01765"
     CDS             3482..4222
                     /locus_tag="P5293_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRR48693.1"
                     /translation="MVTKILSLPQLAIKPDAIIDSLCHENKEAKVTGNQEYLLEITRD
                     IMADLSRICNPQYGYELFNVKMIANDYLEIDKSFVHPECVTFNVGSRIASSFAGAESV
                     AVFVSTAGNAFEHYMRSQKDNILKEFITDAIGSEIPEAVNKILLKELEEQFNRGNDEL
                     HLSNPYSPGYCGWNVAEQQKLFSLLPPEPCGIKLNASSLMTPLKSVSGIIAIGKNVKK
                     TGYKCDECDINNCTRNFRYKQSINNRIK"
     gene            4219..6243
                     /locus_tag="P5293_01770"
     CDS             4219..6243
                     /locus_tag="P5293_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR48694.1"
                     /translation="MTGKELLKKAMKLEEVERPPWVPFVGVHAAHISGIDAATYLKSE
                     ELIVKGIKNAVKLYKADGIPVVFDLQLEAEALGCNLVWTESNPPSVVSHPLAEGLNLS
                     ELDIPSENDGRIGIVMNAARELVKSEPEIAFYALVTGPFTLALHLSGTDIFMQMLTEP
                     QRVHTLLRFCTDVAKTMCNFYKKAGVDVIAIVDPMTSQIGTEQFVEFVAPCASEIFEE
                     IRTIEAFSSFFVCGDARHNIEAMCNCRPDNISVDENVPLDYLRDICLANRISFGGNLK
                     LTSTLLMGTPLDCEKDALECLDIGGNRGYVLSPGCDLPFYTVKENLIAVSNLVRDKYR
                     QDIVRELSRQEIECEPLDIAKYASGDKVKIDVITLDSSSCAPCQYMLDAAVRASESFG
                     DKVIIKEYKIKSEEGLTKMRQLGLKNIPAICIDGRPVFVSKIPPLGDIKKAIMERLSA
                     KENLLQQISYCIEKGKVDAGMAYPPELKGQPGVDELTKMALEAGYSANEILSKAMIPA
                     MGIVGELFKEKKLFVPQVLMSAMAMKSAMKHLKPYFSSGEIKSKGVFIIGTVAGDLHD
                     IGKNLVCMMVEGAGWKVIDLGTDVKTSTFMDAVSENRGSILGMSALLTTTMAEMEKSV
                     TAIHKEYPEQKIIVGGAPVNQVFASKIGADFYAPDPQVAIDYMNSLNLVS"
     gene            6240..7382
                     /locus_tag="P5293_01775"
     CDS             6240..7382
                     /locus_tag="P5293_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HRR48695.1"
                     /translation="MKLSPRERVLAAMNLQKPNNIPLMCQFSIGSMMQQLKPAPAEFW
                     YDGQIFADGLIELCKRFRFDGILVSLHGHNPNWKDDVVRIDNIEEGLQEVIYEDRKEI
                     HSWKDLPLVKFNSRTENKAISSVDVSKEVPSTIDYIPVSQNLHFKLDKNHLFDIFDYI
                     FSKTQGEYSIHGEITSPFDYLLDFLGYENALISLILDPEKCKEILQKFTDGLCTLASE
                     MCEKEIDAVKLSSPFAGMGFISPEHYQEFVLPYESRLIKIIRDKGKHAYIHTCGHIDD
                     RLELMQKSGASGLECLDPPPVGNVDLADAFARIGNCMFIKGNIDSVNTLLFADDTKAR
                     EDILRILKIGREGKGFILSTACSISPMVPASRIHMLRELLSEFEQM"
     gene            complement(7384..8343)
                     /gene="metF"
                     /locus_tag="P5293_01780"
     CDS             complement(7384..8343)
                     /gene="metF"
                     /locus_tag="P5293_01780"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRR48696.1"
                     /translation="MKVTEHIEKSKGRTLFSVEILPPLKGQNIESTFKSLDSLMEFEI
                     PFIDVTYHREEFEFKEFPDGHLERRVVKKRPGTVGICAAIQNRYKVDAIPHVLCGGFT
                     REETENFLIDISFLGIDNVMALRGDAIKSESCFVPEKGGNQHASELVKQISDMNRGIY
                     LDNELLDTSPTNFCIGVAGYPEKHIEAPSMESDIHFLKEKVRNGANYIITQMFFDNRK
                     YFDFVERCRIEGISVPIIPGIKPIATKRQLNLLPLKFKVDLPDELVKMIVKAKDEEEV
                     RQIGVEWSIGQCRELIKAKVPLIHFYATGRAENIVQVCRSLRV"
     gene            complement(8370..11915)
                     /gene="metH"
                     /locus_tag="P5293_01785"
     CDS             complement(8370..11915)
                     /gene="metH"
                     /locus_tag="P5293_01785"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRR48697.1"
                     /translation="MKGIREIVRERILILDGAMGTMLQKYGYGEEVKGNFDLLSLTRP
                     SVIENIHRQYLEAGADIIETNTFSSNAVSQADYNLEDSVYKLNYESAAIARKVANEFT
                     LRDPKKPRFVAGSIGPTNKSSSISSDVNNPGFRAVDFETLRAAYKLQAEALLDGGVNL
                     LLVETVFDTLNAKAALFAIDEIREERGIDIPVMLSGTIADNSGRVLSGQTIESFLISV
                     THFPLFSIGLNCALGAEQLLPYLRRLSVAADICISAHPNAGLPDAFGEYSHTPEMMLE
                     MIYPYFEEGLVNIIGGCCGTTPEHIRLIAGAAKSFFPRNPSTEANNSGDKSLLREKCL
                     KLSGLEPLIVTDKTNFVNVGERTNVSGSRKFLRLIKDGNYREAVEIARAQVESGAQIL
                     DINMDDGMLDGVSEMTKFLNLIGSEPDIARLPVMIDSSKWEIIEAGLKAFQGKGVVNS
                     ISLKEGEESFVQKARAIRRYGAAIIVMAFDEKGQADTFERRVEICRRSYDILTNKAGV
                     PPEDIIFDTNIFPVATGMEEHRDNAVNFFKAARWIRENLPYAGVSGGVSNVSFSFRGN
                     DRVREAMHTAFLYHAVQNGMTMGIVNPETLGIYDEIDKELLELVEDVLLNRREDATER
                     LVAYAGKLGENYKSDENSNPEWRQSSLQERIIHSLVKGIDRYIEIDMEEARGSVVNPV
                     EIIEKYLMTGMNVVGDFFGSGKMFLPQVVKSARVMKRAVAYLLPYIISKNNKDELSNK
                     RAGKILLATVKGDIHDIGKNIVSVVLSCNNYEIVDLGVMVPAEKIVDTAVKEKVDLIG
                     LSGLITPSLGEMVNVVEELERKGLSIPVMIGGATTSKMHTAVRIAPAYSGHVVHVNDA
                     SKAVTVAGSILKMDGKEIYLRNIREEYNNLRENFLNSGVSDRIMTIEEARANKLKLDW
                     ETYIPPAPKVQKVQCIEQSIEELIEYIDWKPFLRLWNVEGEQSAKLMRDAMAMLKKVI
                     DEKWLAARGVYGIFPANTVENDIIEIYSEEGEVIEQFPTLRQLVRKTKSSPNLSLSDF
                     IAPKASGKRDYLGMFCLSAGFGGDEKVAVFEAELNDYNAIMLKALADRLAEAFAELLH
                     KKIRTEYWGYSPDEHLTMDGIFKGEYRGIRPAPGYPACPEHRDKEIIWKLLEVEKNIG
                     AKLTENYAMWPAASISGFYFAHPDARYFGVGKKNF"
     gene            12107..12550
                     /locus_tag="P5293_01790"
     CDS             12107..12550
                     /locus_tag="P5293_01790"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF014694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HRR48698.1"
                     /translation="MEKPTSFQINHLFLEPGVYLRDADEKRSVYTYDIRLVKPTLSYR
                     EAITPQMSHTLEHWLAHYLRIISPIGNEIVYVGPMGCLTGFYILTFKRYTEKYMRDLV
                     VTALQAILEIDEIPGAKPEECGNYTLFDLESTKRRIPEFISLLNK"
     gene            12565..12726
                     /locus_tag="P5293_01795"
     CDS             12565..12726
                     /locus_tag="P5293_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HRR48699.1"
                     /translation="MSLLRVILCIIFPPLAVVDYGCGSVLIVFLLTLAGWVPGVIAAL
                     IIVNRRRPF"
     gene            complement(12728..15184)
                     /locus_tag="P5293_01800"
     CDS             complement(12728..15184)
                     /locus_tag="P5293_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR48700.1"
                     /translation="MKKIHDLLMFLQLTAFILLFNSISLKAQENITFQTPPKEIMELA
                     DYKVAPRVIFDSKDEYMIFSYRDTYQTLAEISQEVMKLAGIRINPRNNMASDLKFENN
                     LKVRKVVCDDGLWEPVQVKGLPQNLRISEKTFSPDEKLLAFTNTTDTDVELWVLDIAQ
                     AEAKRVATGLNGYLDNAFIWLKDSKSLLIWSLPSKRPSLLDPAKDLPKGPSVLEGKGK
                     ESQARTYPDLLKNEIDIANFETLATSELNIVNLEGERKPFLGADIYVNTTLSPDGQYL
                     LVTTIHRPYSFIVPMNMFPTRSSVYDLDGNLIKIIYDKPLFEGRPKGSSSTFPGMRLI
                     NWRADKPASLYYAIALDNGNQAVKVDFRDEIFSWDAPFTSEPKSLFKTGQRFSGVVWG
                     DDKYAVFMDFWAETRSTRTFIFNPSDPNSAPIKLFDRNRQDVYSDPGNFQTRKNQYGR
                     YTLAIENDKLFLIGDGFTKNGQFPFVDQFDLKTFKTKRLYQSLYTDKMEQIRSIEDYK
                     KGILLVNIQSKTEHPNFYFREISKKGDKGLKQLTFFKNPFESIAKVHKEVIKYKRDDG
                     VELSGTLYLPANYDTNSGEKLPLLIWAYPKEFKDANSAGQNKLNPNEFTVPYYGSFVY
                     WVTRGYAVLDDASFPIIGEGDREPNDTFLEQLVADAKAAIDAVDGLGYIDRKKVAVGG
                     HSYGAFMTANLLTHSDLFACGIARSGAYNRTLTPFGFQNEQRNYWDAPEVYNKMSPFM
                     YANKMKTPLLLVHGDSDNNPGTFTIQSERYYQALAGLGAPVRLVLLPKEAHSYVAREN
                     IFHLLWEEDVFLEKYCKGRQ"
     gene            15282..16316
                     /locus_tag="P5293_01805"
     CDS             15282..16316
                     /locus_tag="P5293_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48701.1"
                     /translation="MGKINKFTFLIIFFTFILSFRLLGATQEDTTNNHELTIFVIPSL
                     RDIDWQSPSSLSQTTTKSWISSKFVRHSYYIGHLFIELTSSLLDRPVVTSVRSVGNKE
                     KLRLLYKEKIGMAIIGAPLKGRMESADELVRSIDFTLKKERSIAFIKYSINENAAKRI
                     IEFIDGFSKPDSTSQAPCDIYGGGYWPRYKGEGAGCSAFGMVVMEVAGLKHDYPEWIH
                     SVNIPAELVGGQYNNYIKVKNSAIKNTTAWNDRSGTANIDYFSFSIYDPTLIYNWIIN
                     MRSTCESDTSIILSGFKPAVITRDQGTIPGLYLDARNMHIPLDEPVFKERETFSIFAI
                     PPTTQSRRHK"
     gene            complement(16291..17064)
                     /locus_tag="P5293_01810"
     CDS             complement(16291..17064)
                     /locus_tag="P5293_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48702.1"
                     /translation="MKKFLNTSLLVISMLLLVSGLSSCGFFDDDDTEYEYGKLMVKIE
                     SGYNNRELLVTYSPYGLITGYTVKEEGTIKESYVVDYYSSFVRMTATAGQATGNIRDI
                     YYDTQGRATTVQESNSVLMRFSYNSNYFLTQEIFESNSNPLVEYSYDSDGVISQIVQY
                     DTEGQELEQVSLQDCDVALAYNTVGLVFLDLLYNPYENYRPFLALLGIAGTLPRSYSA
                     PEGRFSGFQYDAMGYISSVIMTVDGVPYEVNFIYDDEIE"
     gene            complement(17088..>17854)
                     /locus_tag="P5293_01815"
     CDS             complement(17088..>17854)
                     /locus_tag="P5293_01815"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRR48703.1"
                     /translation="VLIMALLPIVGTAQNSKSKKPVIGISSTMGDGTDTSVPLTYINA
                     VIRAGGVPVVIPITDDIEILSTILDRIDAVIMTGGEDIDPLKWYGEQPIPQLGEIAPD
                     RDAFDIALIRLTVERGLPLLGICRGEQLLNVAFGGTLYQDLPSQFKGYSVSHRQKAPG
                     KYGTHSIYIEKNSLLHKMILLDSTAVNSFHHQAVKDVAPGFRVTAVSKDGVVEAIEKI
                     GSTSVYGVQFHPEIFTANGIDTFIGIFKNLVQEASK"
     assembly_gap    17855..17954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18096..19481
                     /gene="radA"
                     /locus_tag="P5293_01820"
     CDS             18096..19481
                     /gene="radA"
                     /locus_tag="P5293_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRR48704.1"
                     /translation="MAKVKKAWFCTSCGNESPKWMGRCPACGEWNTLQEEIVSKENPL
                     RGPSTSLRNGSRSTKVEPLSSISCSEENRISLGSTELDRILGGGLVTGSLILIGGEPG
                     IGKSTLSLQIPLRCHNLRTLYVSGEESPSQIKLRAQRLGGEGENCLVLSETLIENVIE
                     KSNEIIPELIIIDSIQTMYSQGVENSPGSISQVRECATLLLRYCKETNIPVIVIGHIT
                     KDGMIAGPKILEHIVDVVLQFEGDTRHSYRILRSIKNRFGSTAELAIFEMINSGLREV
                     TNPSEMLIPMHEENLSGIAVASMLDGTRPFLIETQALVSTAAYGTPQRSATGFDVRRM
                     NMILAVLEKRAGFHLSAKDVFLNIAGGLKISDPACDLAIACAILSSNFDISISQKICF
                     SGEIGLSGEIRPVSQIEKRIDEAGKLGFETIYISSYNHPSIEKRSASAANKIAIHKVK
                     DIPELVRLVFR"
     gene            complement(19474..20430)
                     /locus_tag="P5293_01825"
     CDS             complement(19474..20430)
                     /locus_tag="P5293_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRR48705.1"
                     /translation="MRFLGEIISIGVAVSWTLSALFFEYAGNKIGSLIVNILRLFFAF
                     FLLGGLLLILTGSPLPIHADRDTWIWMILSGLVGFVFGDLCLFHSYLLITARFSQLLM
                     TLAPPFAALSGWFILGERLTLQAFLGMLVTLTGIAISVLKRDSSSSVDGKKNTVNGRI
                     KLNMPLKGFLLGIGGALGQGLGIVLSKQGMNYYQASAQGATVEHYIPFAATQIRVITG
                     LIGFFLIIVLSGRFRNLVDGLMNSKGMKATFAGSIFGPFIGVSLSLMAVQYTNAAVAS
                     TIMATTPILILIPYVLIYKKKVTPVELLGALLSVGGVAIFFI"
     gene            20577..>20917
                     /locus_tag="P5293_01830"
     CDS             20577..>20917
                     /locus_tag="P5293_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR48706.1"
                     /translation="MDRKVLLTVIITTLICFYNDSYSQENKKWTLRECIDYALSNNVS
                     IKSAEISVKSSLEDLQQAKASMLPSLSLSSSQNFSAQKQERTDESFVTKGSYTGSYAL
                     NTSVTLYSGGK"
BASE COUNT         6463 a         4297 c         3823 g         6134 t
ORIGIN      
        1 atgcgttttt tggagaaaaa gactatcaac aacttgcaat tgtaaaatac tttacaaggc
       61 aattaggcta tcctgttcaa ataattgcct gcccaattgt ccgcgaagac gatggtctcg
      121 cgatgagctc aagaaatacg cttcttacac ctgaacaaag aaaggtcgct cccttgattt
      181 atcaggcact ccttaatgca gcagctctgg cgaaagaaga aaaaatcaca cctgaagaac
      241 tgattaaaat taccataaat aaaattaaca actctccaca gcttgaagtt gaatacgttg
      301 aaattgtcga ttccgcaact ttacagcctg tttcactatg ggacgaagca gaagaaatac
      361 aaatgtggac tgctgtattt gccggaaaga caaggttgat tgacaacatt aaattgaaat
      421 aagatgcaac tcgaaataat gaaatccaag attcacagag cccgtgtaac agaagcgaat
      481 cttaactata tcggcagtat tactatagat gcagatcttg ctgatgcagc cggtctgtac
      541 ccaaacgaaa aggtgtcagt agtaaacata aacaatggtg aaagaattga gacatatgtc
      601 attatgggag agagaggaag cggcaaaatt ggaattaatg gagctgctgc tcataaattt
      661 gccattgacg atcttgttat aataatggca tatgccacca tgtcacctca agaagcaatt
      721 gcatttaaac caaagataat atttccagat tcgaaaagca ataagttgat cccatgaaca
      781 attaatcgaa taaatgaaaa aattctactc ctcacttaaa tacttacttc tgctttcttt
      841 ggcgggtatt ttactgtggt tttcatttaa aggggtgaac tggttagatt ttctgaatgg
      901 aataaaggat gcaaattatt ggtggatact tgcttcaatg acaataagca ttttagcttt
      961 ttttatcagg gctgtaagag gaagaatgat aatgcaaact ctgggtaaac cagtcaagct
     1021 aagagattca tgggatggtg ttaatatagg ataccttact aatttcgcag caccaagagc
     1081 aggcgaactt gcaagatgcg gcgtaattgc aaaaaaaagt ggaataccag ttgaatctgt
     1141 agttggatca gtggttctcg aaaggagtat tgatctgctt tccctgatta ttctggtaat
     1201 attagtggtt ttcctttcat gggaacagtt tggaacattt attaataagg aaatactcgg
     1261 cgcacttccg ggaaaattct cttccaacct gattttggtg gcatcagtga ttatacttgc
     1321 ctttgcaata tttatctata ttgtcttcaa atacagagag cgccatccta tatttaaaaa
     1381 aattgttaaa ataacaaagg gtctttttga cgggcttgtt tcaggcttta ggatgaaaca
     1441 aaagtggctg tttattctgt taacaatttt actctggaca tgctattggc taatgagcct
     1501 tactactatt tatgcttttc ccaaggtcgg ctttttagga gctacggatg ccctgttcct
     1561 catggtagtc ggaagccttg gatggcttat tcctgtacag ggaggtatag gagcttttca
     1621 ttttgtgaca actctcgctc ttgcacaagt ctacggcata tcgcaaacca caggaattgt
     1681 ctttgctact atttcacatg aatcacaggc attaaccatg ataatatgcg gcactctgtc
     1741 acttgtaagt ttttcattat ctaaacggaa aaaggaggtt gtcaaatgat gaaaaaaatt
     1801 ttattaactc tctttttaat cttcccaatt accgtatttg ccgagcacca gaaaaataat
     1861 ggcagtgaca tttatcttat aatctcatcc tacaaccctg acactcagag gatgtctgac
     1921 tttattacag atttcgaaaa tattgtaaca gaaagaggag aaaaagccga tattgtcatt
     1981 gaagaccttg gttgcaagag cttcagcgaa gaatctttca tgtggaagaa tcagatgaaa
     2041 aacaccttaa acaagtttag aaaatcaaat ctcaaggcta ttatactgct cggccaagag
     2101 gcatgggctt catttctctc tcaagactca attccaaaag gagtgccctt tttctcatcc
     2161 tttgcttcag taaatggcat tgaacttcca aaagattctg ttggccatga gtggcttccc
     2221 gagtctgtga atactgtaga aaaagccaag aagctagggc atggaggagg atatctcaat
     2281 cattatgatg tgactgccaa tataaaactt atcaaaaaac tttatccaaa gacagacaat
     2341 atcatttttg tatcagataa cacctatgga ggggtttcac ttcaagctct cgtgagaaag
     2401 gaaatgaaac aattccctga aataaaactc acacttttgg atggcagaaa gtttacaatt
     2461 caagaggctc agcaaataat gagacgtcta ccacaaaaca gtgcaattct tatgggcact
     2521 tggagagcaa agaaagacgg aataggcctg acaaatacat ctctactcaa acttcgacaa
     2581 gcaaatgaaa atgttccaat attcacaatt tcaggcaccg gtttcggtct tgttgccgtg
     2641 ggtggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaact gattgaagcc
     2761 aaggaacgag cagaacaagc tgacaggctc aaaagtgcat tccttgcaaa tatgagccat
     2821 gagatacgaa cacctcttaa tgcaataatt ggattttcaa atcttttatg ttcagaagat
     2881 ccttctcctg aagagagatc aaagtacaac agcttgatag caaaaaactc tgatatactg
     2941 ctgactctca ttaacgatgt gctcgatatt tcaagacttg agacagatca gatgcacttc
     3001 acataccagc aggcagatct tctgtcaata tgcaatcatg taatcaacag tatcgggcat
     3061 cagaaaaaac ctgaaattga ttatgtgatt gatccagaat gcaagagtta tatagtcgaa
     3121 acggataccc agaggctgat acaggtactt ctcaatcttc taacgaatgc aaataaattt
     3181 acagaaaaag gatctataac tttaaaatat gttgtagatg agaagttgga agttgtcact
     3241 ttttcagtaa ccgataccgg ttgtggaatt ccaagaaata aatttgacag cgtttttgaa
     3301 cgttttggca aactcaacga attttcacaa ggaacaggtc ttggactggc tatttgtact
     3361 caaatagtaa aaaaatttgg cggcaagata tggattgacc ctgaatatgt ttcaggggca
     3421 agattttgct ttacaatacc cataaagcac aacaatactt aaaaacaata tttacaggca
     3481 aatggttact aaaattttaa gtttgccaca acttgccatt aagcccgacg caattattga
     3541 cagcctttgt cacgaaaaca aagaggctaa agttaccggc aatcaggagt atcttctgga
     3601 aataaccagg gatattatgg cagacctctc ccgaatatgc aatcctcaat atggatacga
     3661 acttttcaac gtaaagatga ttgcaaatga ttatttggaa atagataaat catttgtgca
     3721 tcctgaatgt gtaacattca atgtgggaag caggattgca tcttcctttg ccggagcaga
     3781 aagtgttgct gtttttgtct ctacagccgg aaatgctttt gaacactata tgcgctccca
     3841 aaaggataac attctaaagg aatttattac agacgcaata ggtagcgaaa tacctgaagc
     3901 tgtcaacaaa attcttctta aagagcttga agaacagttc aacagaggta atgatgaact
     3961 tcatttatcc aatccttaca gccccggata ttgtgggtgg aatgtggctg aacagcagaa
     4021 actattttca cttttacctc cggaaccttg tggaattaaa ttaaacgcct cgtcattaat
     4081 gacccctcta aagtctgtaa gcggtattat tgcaattgga aaaaatgtca aaaaaacagg
     4141 ttataaatgc gatgaatgcg atataaataa ttgtacacga aacttccgat ataagcaatc
     4201 aataaacaat agaataaaat gacaggtaaa gaattattga aaaaggccat gaaactcgaa
     4261 gaagttgaga ggccaccctg ggtgccattt gtaggggtac atgctgcaca tatttcaggc
     4321 atagatgcag ccacctattt aaagtcagaa gagcttattg taaaaggcat aaaaaatgct
     4381 gtgaagctgt ataaggctga tggtatacct gttgtgtttg accttcaact tgaagcagaa
     4441 gctttgggtt gcaaccttgt ttggacagaa agcaatcctc cttcagttgt ttcacaccct
     4501 ctcgcagaag ggttgaattt aagtgagctt gacattccat ctgagaatga tggaagaata
     4561 ggaattgtta tgaatgctgc aagagaactt gtaaaaagtg aacctgaaat tgctttctac
     4621 gctcttgtta ccggaccttt taccctcgcc ctgcatctca gcggaacaga tatattcatg
     4681 caaatgctta ctgaaccaca acgtgttcac actctactgc gattttgcac tgatgttgcc
     4741 aaaaccatgt gtaactttta taaaaaagcc ggtgtggatg ttatagcaat tgttgatccc
     4801 atgacaagtc agataggtac agagcagttt gttgaatttg tagctccatg tgcctcagag
     4861 atatttgaag agattcgcac tattgaagct ttctcttctt tctttgtatg tggagatgcc
     4921 cgacataata tcgaagctat gtgcaactgc aggcccgaca atatctctgt agatgaaaac
     4981 gttcctcttg attatctaag agacatatgc ctcgccaaca gaatcagttt cggaggcaat
     5041 ctcaaactta cttccactct gctgatgggt acacctctcg actgcgaaaa agatgccctg
     5101 gagtgtcttg acattggcgg gaacaggggg tatgtactct ctccaggatg tgaccttcca
     5161 ttttacactg taaaggaaaa tttaattgca gtttccaatc tagttagaga caaatataga
     5221 caggatatag taagggaatt aagtcggcaa gagattgaat gcgaaccttt ggacattgca
     5281 aaatatgcct caggagacaa ggtcaaaata gatgttatca cattggactc ttcctcctgc
     5341 gctccgtgcc aatatatgtt ggatgctgca gtcagagcat ctgaatcttt tggtgacaaa
     5401 gtcataataa aagaatataa gataaagtct gaagaaggac ttacaaagat gaggcagtta
     5461 gggcttaaaa acatccctgc tatttgcata gatggaaggc cagtatttgt atccaaaata
     5521 ccgccgcttg gagacatcaa aaaggctatt atggagagac tttctgcaaa agagaatttg
     5581 ttacaacaga tctcctattg catagaaaag ggcaaagttg acgctggcat ggcttatcct
     5641 cctgaattga aaggtcaacc gggcgtagat gaacttacca aaatggcact ggaagcaggt
     5701 tactctgcaa atgaaatact ttctaaagct atgattcctg ccatgggaat agtaggtgag
     5761 ctttttaagg agaaaaaact ctttgtccca caagtactta tgtctgccat ggcaatgaaa
     5821 tctgctatga agcatctgaa accctatttt agctccggag agattaagag taaaggagtc
     5881 tttattattg gaacagtcgc gggtgatttg catgatattg gcaaaaatct ggtatgtatg
     5941 atggtagaag gggccggatg gaaagttatc gaccttggca cagatgttaa aacctcaaca
     6001 tttatggatg ctgtctctga aaacagaggg tcaatattag gaatgtcagc tttactgaca
     6061 actacaatgg cggagatgga aaaatcggta acagctattc acaaagaata tcctgagcaa
     6121 aagataatcg tagggggagc accagtaaac caagttttcg cttcaaagat tggtgctgat
     6181 ttttacgctc ctgatcctca agtagcaatt gattatatga attctttaaa cttagtatca
     6241 tgaaactttc accaagagag agagttctgg cagcaatgaa tctccagaag ccaaataata
     6301 ttcctctgat gtgtcagttt agcatcgggt caatgatgca gcagttaaag ccggctcctg
     6361 ccgaattctg gtacgatgga caaatctttg cagatggatt gatagaactg tgcaaacgat
     6421 ttcgcttcga tgggatactg gtgagtcttc acggccataa tccaaactgg aaagacgatg
     6481 tcgtaagaat cgacaatatt gaagaaggat tacaggaagt aatctatgag gacagaaaag
     6541 agatacactc gtggaaagat cttcctctgg tgaaatttaa cagtaggacc gagaataaag
     6601 ccatttcaag tgtagacgtt tcaaaggagg tgccgtctac tattgattat atcccggtat
     6661 ctcaaaatct tcatttcaaa ctcgacaaaa atcatctttt cgatattttt gactatatct
     6721 tttcaaagac acaaggcgag tactccatac acggagagat aacgtctcct tttgactacc
     6781 ttcttgactt tctgggatat gaaaatgcac tcatatcact gattcttgat cccgaaaagt
     6841 gtaaggagat tcttcagaaa ttcacggacg gactttgtac tcttgcctca gaaatgtgcg
     6901 aaaaagagat agatgctgtc aaactctcct ctcctttcgc cggcatggga tttatctctc
     6961 cggaacacta tcaggagttt gtactcccat atgaaagccg gttaatcaag ataatcagag
     7021 ataagggcaa acacgcctat attcatacct gtggacatat agacgacagg ctggagttga
     7081 tgcaaaagag tggcgcatcc ggtcttgaat gtctggatcc accacccgtt ggaaatgttg
     7141 accttgcgga tgcttttgca agaattggaa actgcatgtt catcaaggga aatattgact
     7201 ctgtaaacac ccttcttttt gccgacgaca ccaaggcacg cgaagatatt ctgagaatct
     7261 tgaaaatcgg ccgagaggga aaaggattta ttttaagcac tgcctgctcc atttccccca
     7321 tggttcctgc ctcaaggatt cacatgttaa gagaattact gtcggagttt gaacagatgt
     7381 aacctacact ctaaggcttc tacagacttg tactatgttt tctgctctgc ctgttgcata
     7441 aaagtgtatt aaaggaactt ttgccttaat aagttctctg cattgcccaa tagaccattc
     7501 aacgcctatt tgtctgacct cttcttcgtc tttcgctttt actatcattt tcacaagttc
     7561 atccggaaga tcaaccttga atttaagagg aagcaggttt agctgacgtt tggttgcaat
     7621 tggtttaata ccaggaataa taggaactga tattccctca atcctgcatc tctccacaaa
     7681 atcgaaatat ttacgattgt caaaaaacat ttgagtaatt atatagtttg caccatttcg
     7741 gactttttcc ttcaaaaaat gaatatcact ttccatgctt ggcgcttcaa tatgtttctc
     7801 tgggtatcct gctacaccaa tgcagaaatt agtgggagaa gtatcaagca gctcattatc
     7861 aagatagatt cccctgttca tgtcggaaat ttgcttcaca agctcactag catgctgatt
     7921 accacctttt tcgggaacga aacaactctc gctcttaatg gcatctcctc taagggccat
     7981 tacattatct atccctagaa aactaatatc aataagaaaa ttttcagtct cctcccttgt
     8041 aaaacccccg cacagaacat gcggaattgc atccacttta tatctgtttt gtatagctgc
     8101 acatatacct actgtcccgg gtcgtttctt gacaacgcgc ctttcaagat gaccatcagg
     8161 aaattctttg aactcaaact cttctctgtg ataagttaca tctataaaag gaatttcaaa
     8221 ctccatcaga gagtccaggc ttttaaatgt cgattcgata ttttgtccct taagtggcgg
     8281 caatatctct accgaaaaca gtgtcctgcc tttagatttc tcaatatgct ccgtaacctt
     8341 catttcacca aattattact catttctttt taaaaattct tttttccaac accaaagtac
     8401 cttgcatccg gatgagcaaa ataaaaccct gaaatggatg cagcaggcca cattgcgtaa
     8461 ttttccgtca atttcgcccc aatatttttt tcaacttcga gcaatttcca aataatttcc
     8521 ttatctctgt gttcaggaca cgcaggataa cctggtgcag gtcttatgcc tctgtactca
     8581 cccttaaaaa ttccatccat ggtcagatgc tcatcaggag aatatcccca gtactctgta
     8641 cgtatttttt tgtggagaag ttccgcaaaa gcttctgcaa gccggtctgc aagagcttta
     8701 agcataatag cgttataatc attcaattcc gcctcaaaga ctgcaacttt ctcatcccca
     8761 ccaaacccgg ctgatagaca aaacatgcca agataatccc tcttgccact cgctttggga
     8821 gcaataaaat ctgacaatga gagattaggg ctgctctttg tttttcggac caattgtctt
     8881 agtgtaggaa actgttctat aacttccccc tcttcactat aaatctcaat aatgtcattt
     8941 tcaacagtat tagcagggaa tataccataa accccccttg cagctagcca tttttcatca
     9001 attaccttct taagcatagc cattgcatcc ctcatgagtt tagcagattg ttctccttca
     9061 acattccaaa gccgcaaaaa aggtttccag tctatgtatt ctatcaactc ttcaattgac
     9121 tgttcaatac attggacttt ttgaacttta ggagctggag gtatataagt ttcccagtca
     9181 agtttgagtt tattggctcg tgcctcttct attgtcataa ttctgtcact tactccactg
     9241 ttcaaaaaat tttctctgag attattatat tcctccctaa tattccttaa ataaatttct
     9301 ttcccatcca tcttgagaat tgagcctgct acggtaacag cctttgacgc atcattcaca
     9361 tgaacaacat gtccactata agcaggagca atcctgacag cagtatgcat ctttgaagtc
     9421 gtagctccgc caatcatgac cgggatactc aaaccctttc tttccaactc ctcaacaaca
     9481 tttaccattt ctccaagaga aggagttatt aaaccgctta aaccaatcaa gtcgactttt
     9541 tcttttactg ctgtatcaac tatcttctct gcaggcacca taactccgag atcaacaatt
     9601 tcataattat tgcaggagag caccaccgaa acaatattct ttccaatatc gtgaatatcc
     9661 ccctttacag tagccagcaa tatttttcct gcccttttgt tcgaaagctc atctttgttg
     9721 tttttagaaa ttatgtatgg cagcagatat gctacagccc ttttcatgac tctcgccgat
     9781 ttaactactt gaggaagaaa cattttcccg ctgccaaaaa aatcccctac aacgttcatc
     9841 cctgtcataa gatatttttc tataatctct acgggattaa ctacacttcc tcttgcctct
     9901 tccatatcaa tctctatata ccggtcaata cctttgacca aagaatgaat gattctttct
     9961 tgaagtgaac tctggcgcca ttctggattg gaattttcat ctgacttgta gttttcaccc
    10021 aacttacctg catatgccac aagcctttca gtagcatcct ctcttctgtt aagcagtaca
    10081 tcttcaacca gttccaacaa ttctttatct atctcatcat atattccaag agtttcagga
    10141 ttgactatgc ccatagtcat gccattttga actgcatgat acagaaaagc cgtatgcatc
    10201 gcttctctta ctctgtcatt tccgcgaaaa gagaaggata cattacttac gcctccactc
    10261 actccagcat aaggtaaatt ttctcttatc catctggctg ctttgaaaaa gttgacagca
    10321 ttgtctctgt gttcttccat ccccgtagcc acaggaaaaa tatttgtgtc aaaaattata
    10381 tcttccggag ggacaccggc tttgtttgta agaatatcat aagatctgcg acagatctca
    10441 actctccgct caaaagtatc tgcctgaccc ttttcatcaa aagccatgac tataatagct
    10501 gccccatacc ttctaatagc gcgtgctttc tggacaaaac tttcctctcc ctccttgaga
    10561 gaaatagaat ttacaactcc ttttccctga aatgccttta aaccagcctc tattatctcc
    10621 cattttgaac tgtcaatcat tacaggaagc cttgcaatat cgggctcgga accgatcaag
    10681 ttcaaaaact ttgtcatctc actaactcca tcaagcattc cgtcatccat attgatatcg
    10741 agtatctgag ccccactttc aacctgcgcc cttgctatct ccactgcttc ccgataatta
    10801 ccatccttaa tgagtcgcaa aaattttctt gaaccggaaa catttgttct ctcgcctacg
    10861 tttacaaaat ttgtcttgtc agtaactatc agaggttcaa gtccagataa ctttaaacac
    10921 ttttctctca aaagggattt gtcccctgaa ttatttgcct cagttgaagg atttcttgga
    10981 aaaaaactct tagctgctcc ggcaattaat cttatatgtt caggggttgt tccacaacat
    11041 cccccaataa tatttaccag cccctcctca aaatatggat atatcatttc gagcatcatc
    11101 tcaggggtat ggctgtattc accaaaagca tccggcagac cggcattggg gtgtgcagaa
    11161 atgcaaatgt ctgcagccac ggaaagtcgt cttaaataag gaagaagttg ttcagctcca
    11221 agagcacagt tcagccctat gctgaagagt ggaaaatgag ttaccgaaat taggaaagat
    11281 tcaattgtct ggcctgaaag aactctgccg gagttatcag caatcgttcc tgataacata
    11341 acaggaatgt ctattcccct ctcttccctt atctcatcaa tagcaaaaag agctgctttg
    11401 gcattcaaag tatcaaaaac agtctcaaca agcagtaaat ttactcctcc gtcaagcagg
    11461 gcttcagcct gtagtttgta tgctgctctc agagtttcaa aatctacagc tctaaacccc
    11521 ggattgttta catcagaaga gatgctggaa cttttgttcg ttggacctat tgacccagcc
    11581 acaaatctcg gtttttttgg atctctcagt gtaaattcat ttgcaacttt tctggcaatt
    11641 gcagcagatt cataatttaa tttatataca ctatcctcta aattataatc cgcctgtgaa
    11701 actgcattag aagaaaaagt gttggtctct ataatatcag cccctgcctc gagatattgt
    11761 cgatgaatgt tctctataac agatggtctg gtcaacgaaa gcagatcaaa attgccctta
    11821 acctcttctc catacccata tttctgcaac attgttccca ttgctccatc aagaataaga
    11881 atcctctccc tgactatttc tcttatacct ttcataaata aaaaacctct gctttttggc
    11941 agaggatata tataaaattt tattaccagt taaatacaaa aaccccctgc agctttcttc
    12001 tgcaacattt gcatggacat aaacataatg tggttgatta aaatcatata ggatttcgga
    12061 tattttcaca aaaatggaaa ctctttttta attttacaaa aaaaatatgg aaaagcctac
    12121 ctcatttcaa ataaaccacc tgtttcttga acctggagtt tatctcagag atgcagatga
    12181 gaaaagaagc gtttacacct atgacatcag gcttgtaaaa cccactctat cataccgaga
    12241 agcaatcacc ccacaaatgt ctcacacctt ggaacattgg cttgcgcatt atctcagaat
    12301 tatctctcca attggtaatg aaatcgtata tgtgggtcca atgggctgct tgaccggttt
    12361 ttacatcctt acatttaaaa gatatacgga aaaatacatg agagatttgg tagtaactgc
    12421 tttgcaagca atacttgaaa ttgacgaaat ccccggtgca aaaccggaag aatgcggcaa
    12481 ctatactctc ttcgatctcg aaagtacaaa gagacgaatt cctgaattta tatctttatt
    12541 aaacaaatag ccacaacctt aatcatgtca ctgctgagag ttattctttg tattattttc
    12601 cctccccttg ccgttgtaga ttatggttgt ggatctgtac taattgtatt tctgctgacc
    12661 cttgccgggt gggttccggg tgtaatcgcg gctctgatta tcgtaaaccg gagacgtcca
    12721 ttctgactta ctgcctccct ttacagtact tttcgagaaa tacgtcctct tcccacaaaa
    12781 ggtgaaaaat attttccctt gccacatagc tatgagcctc ttttggaagc aacacaagtc
    12841 ttacaggagc accaagtcct gccaaagctt ggtaataacg ctctgactga attgtgaaag
    12901 ttccgggatt gttgtcagag tcaccatgaa ccagcagtaa tggagttttc atcttgttgg
    12961 catacatgaa tggagacatt ttgttataca cttcaggcgc atcccaataa ttacgctgtt
    13021 cgttctgaaa accaaaagga gtaagtgttc tgttgtatgc accacttctc gctattccac
    13081 atgcaaaaag gtcagaatgg gttaatagat tagcagtcat gaatgctccg taagagtgtc
    13141 ccccaactgc tactttcttc ctgtcaatat agcccagccc gtcgacggcg tcaattgcgg
    13201 ctttggcatc tgccacaagt tgttccagaa atgtatcatt tggttccctg tctccttcac
    13261 caataattgg gaatgatgca tcatcaagca ctgcataccc tcttgtaacc caatacacaa
    13321 aagaaccata gtaaggaact gtaaattcat tcggatttaa cttgttctgt cctgcagaat
    13381 tagcatcctt aaactcttta ggatatgccc agataagcag tggcagcttt tctccactgt
    13441 tagtatcgta gtttgcaggt agataaagtg ttcccgacaa ctcaacacca tcatctcttt
    13501 tatactttat tacctcctta tgcactttag ctattgactc aaaaggattc ttaaagaaag
    13561 tcaactgttt taatcccttg tctccctttt tgctaatttc tctaaaatag aaattaggat
    13621 gttcggtttt agactgaata tttaccagaa gtattccctt tttataatct tcaattgaac
    13681 gaatctgctc cattttatca gtatagagag attgatacag tcgtttggtt ttaaaagtct
    13741 taagatcaaa ttgatctaca aaaggaaatt gaccattctt tgtaaaacca tctcctataa
    13801 ggaaaagctt gtcattctca attgccagag tgtatcttcc atactgattt ttacgggtct
    13861 gaaaattacc aggatctgaa tacacatcct gtctatttct gtcaaaaagc ttaattggag
    13921 cagaatttgg atcagaaggg ttgaaaataa atgttctcgt acttctcgtt tctgcccaaa
    13981 aatccataaa aactgcatat ttgtcatctc cccaaacaac tcctgaaaat ctttggccgg
    14041 ttttaaaaag agactttggt tcacttgtaa aaggtgcatc ccaggagaat atttcgtctc
    14101 tgaaatcaac ttttacagcc tggttaccat tgtcaagtgc tatggcataa tacaaagatg
    14161 ccggcttgtc tgcacgccag ttaattaatc tcataccagg gaaagttgaa gatgaccctt
    14221 taggcctccc ttcaaacaag ggcttgtcat atattatttt tatcaaattg ccgtcaagat
    14281 cataaacaga acttctggtt gggaacatat tcataggaac aataaaagag taaggcctgt
    14341 gtatagtggt cacaagcaga tactgaccat ctggagataa tgttgtgttc acataaatat
    14401 cagcacccaa aaatggttta cgttctcctt caagatttac aatattcagc tctgaagtag
    14461 caagcgtttc aaaatttgca atgtctattt cattttttaa aagatctgga taggttcttg
    14521 cctgcgattc ctttcctttg ccctccagaa cacttggccc ctttggcaga tctttggcag
    14581 gatcgagcaa tgaaggtctt ttggaaggca acgaccatat caacaatgat ttactgtctt
    14641 ttaaccatat aaaagcatta tcaagatatc cgttgagacc tgtggcaact cttttagcct
    14701 ccgcctgagc aatgtctagc acccataatt caacatctgt gtcagtagta ttagtaaatg
    14761 caagtaattt ctcatcagga gagaaggtct tttcagatat tctcaaattt tgaggtaacc
    14821 ctttaacctg gacaggctcc catagtccgt catcacaaac aacctttctt actttcaaat
    14881 tattttcaaa tttaagatct gaagccatat tgtttcttgg attgattctg atacctgcaa
    14941 gtttcataac ttcctggctt atctctgcca gagtttgata ggtatctctg taagaaaaaa
    15001 tcatgtattc atctttcgaa tcaaaaatta ctctaggagc cactttgtaa tcagccaact
    15061 ccataatttc ttttggcgga gtttggaaag taatattctc ctgtgctttt aaagaaatgg
    15121 aattaaataa taagataaat gcagtcaact gcagaaacat taatagatcg tgaatttttt
    15181 tcatagaata tcatttatat tattgtaatt gtaataatag caaatttctt aatacaaaag
    15241 atagattttt gtaaattagc aaggattatt atatcaagat tatgggcaaa attaacaaat
    15301 tcactttcct cattatcttc tttacattca tcttatcctt ccggttatta ggagctacac
    15361 aggaagacac taccaataat catgagttaa ctatatttgt cataccatca cttcgtgata
    15421 ttgactggca aagtccttcc tcactatctc aaacaactac caaaagctgg atatcatcaa
    15481 aatttgtcag acactcttac tacattggac atttatttat cgaattaaca tcttccctac
    15541 tcgataggcc ggttgtaaca tctgtgagat ccgttggcaa caaagaaaag ttgagacttc
    15601 tatataaaga aaaaataggg atggcaataa taggtgctcc tcttaaggga agaatggaaa
    15661 gtgccgatga gcttgtgcgt tccattgatt tcactctgaa aaaagaaaga agtattgctt
    15721 tcataaaata ttcgataaac gaaaatgctg caaaaagaat tatagaattt atcgatggat
    15781 tctcaaaacc tgactcaacc tctcaagctc catgcgatat atatggagga ggatattggc
    15841 caagatacaa gggagaaggt gccggctgct ctgcattcgg gatggttgta atggaagtgg
    15901 caggtctgaa gcatgattac cccgaatgga tacattcagt caacataccg gccgaactgg
    15961 ttggaggaca gtataataat tacatcaaag ttaagaacag tgccattaaa aatacaactg
    16021 cctggaatga cagaagtggt actgccaata tagattactt ctcattttca atctatgatc
    16081 ctactctcat atataattgg ataataaata tgcgttcaac ctgcgaaagt gatacatcta
    16141 taattctttc aggatttaaa cctgctgtta taacaagaga ccagggaact atccccggtc
    16201 tctatctgga tgcacgcaat atgcacattc ctctcgatga gcctgtcttc aaagaaagag
    16261 agacgttctc aatctttgcg ataccaccga ctactcaatc tcgtcgtcat aaataaagtt
    16321 tacctcataa ggcacaccat ctactgtcat aatcacactt gaaatatacc ccatggcatc
    16381 atactgaaat ccggaaaatc tcccttcagg agcagaatag cttctcggaa gagttcctgc
    16441 tatgcccagc agggcaagaa atggcctgta gttttcatac ggattgtaca acaagtcaag
    16501 aaaaactaat ccgacagtgt tatatgcaag agcaacatca caatcctgaa gactaacttg
    16561 ttcaagttcc tgaccttctg tgtcatattg aactatctgg gatattactc catcagagtc
    16621 ataagagtac tcaacaagag ggttggaatt actttcaaat atctcctgag tcagaaaata
    16681 attagaattg tatgaaaacc tcattaagac agaattactc tcctgaacag ttgttgctct
    16741 gccctgggta tcataataaa tgtcacgaat attacctgtg gcttgtcctg cagttgcagt
    16801 cattcttaca aaggaagaat agtaatctac aacataactt tccttaatag tgccttcttc
    16861 cttaactgta taaccggtaa ttaatccata aggcgaatat gtgaccagta actctctgtt
    16921 attgtatccg ctttcaattt ttaccatcaa cttgccatat tcatactcag tgtcatcatc
    16981 gtcgaaaaag ccacatgatg acagtcctga tactaataaa agcatgctta tgacaagtag
    17041 agaagtgttt agaaattttt tcattttaaa tatattagtt taattatcta cttacttgct
    17101 tcctgtacaa gatttttgaa gattcctata aatgtgtcaa tcccgttggc tgtaaatatt
    17161 tcaggatgga actgaacacc atatacacta gtgcttccga ttttctctat agcttcaacc
    17221 accccatctt tagatactgc agttactcta aaacctggtg caacatcctt aacggcctga
    17281 tgatgaaaag agttcactgc tgttgagtca agtaaaatca tcttgtgaag cagtgaattt
    17341 ttctcaatat atatagaatg agtgccatat tttccaggtg ctttctgtct gtgagacact
    17401 gaataacctt tgaactgaga gggcaagtcc tgataaagag tcccaccaaa ggccacatta
    17461 agaagctgtt cacccctgca aatacctaac aacggaagcc ctctctccac agtaagtctt
    17521 ataagtgcta tatcaaaagc atctctgtcg ggagctattt ctccaagttg aggtataggc
    17581 tgctctccat accatttgag agggtcaatg tcttctcctc cggtcattat tacagcatca
    17641 attctatcta aaatagttga gagaatttca atgtcatcag taatcggtat aacaacaggg
    17701 acacctcctg cccttataac agcatttata taggttaatg gtactgaagt atctgtaccg
    17761 tcacccattg tcgaagagat gccaatcacc ggctttttcg acttagaatt ctgcgctgtg
    17821 ccgactattg gcaacaaagc cataatcaac actgnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnaaatag ataatctgct atataacttt ttcatgtttt tatattttac
    18001 tatacgtcaa ttcctgcagt cttgctacaa atatagtgga aaataatcat cattttatta
    18061 tatatttgtc atgatagatt caaaaataag aggatatggc aaaggtcaag aaagcatggt
    18121 tctgtacatc ctgtggaaat gaaagtccaa aatggatggg cagatgtcct gcgtgtggtg
    18181 agtggaacac tcttcaagaa gagattgtaa gtaaagagaa tcctctcaga ggaccttcga
    18241 catcattaag aaatggaagc agatcgacaa aagttgagcc attgtcttca atttcctgtt
    18301 cagaagaaaa ccgcatatct cttgggagca cagaacttga cagaattctt ggaggtggtc
    18361 ttgtaactgg atcgcttata ctaatcggag gagaacccgg aatcggtaaa tctactctgt
    18421 ctcttcaaat acctcttcgc tgtcataatt tgagaacttt gtatgtgtca ggagaagaaa
    18481 gtccatcaca gataaaactc agagctcaac gccttggtgg agagggggag aattgtcttg
    18541 tactgagcga aactctcatc gaaaacgtta ttgaaaagtc aaatgagatt atacccgagt
    18601 tgataattat tgactctatt caaactatgt attctcaggg tgtagaaaac tctccaggct
    18661 caatttctca agtcagagag tgcgccactc ttcttctcag atattgcaaa gagacaaata
    18721 ttcccgttat tgtcataggt catataacca aagatggaat gattgccgga cctaagatac
    18781 tcgaacatat cgtcgatgtg gttcttcagt ttgaagggga caccagacac tcctacagaa
    18841 ttcttagaag tatcaaaaac cgtttcggat ctactgcgga acttgcaatc tttgaaatga
    18901 tcaattccgg tctgagagaa gtgacaaatc cttctgaaat gcttattccc atgcatgaag
    18961 aaaacctttc cggaatagca gtagcttcta tgctcgatgg tacgagacct ttcctgatag
    19021 agacacaggc attagtgagc acggctgctt acggtacacc tcaacgatct gctacaggtt
    19081 ttgatgtaag gaggatgaac atgatacttg ctgtcttgga aaaaagagca ggatttcacc
    19141 tttctgccaa agacgtgttt ttaaatattg caggaggtct taaaatatca gaccctgctt
    19201 gcgatctcgc cattgcctgc gccattcttt catccaactt tgacataagc atttcccaga
    19261 aaatttgctt ttctggagag ataggtctca gtggagagat acggcctgtc agtcaaattg
    19321 aaaaaagaat tgatgaagcc ggcaaacttg gatttgagac aatatatata tcctcctaca
    19381 atcatccttc tattgaaaaa agaagtgcct cagctgcaaa caagattgca attcataaag
    19441 tgaaagatat tccggaactg gtcagacttg ttttcagata aagaaaatag caactcctcc
    19501 tactgagagc agagcaccca gaagttctac gggagtaact tttttcttgt aaatcagcac
    19561 ataaggaatg agaatcagga taggcgtggt tgccattatg gtagaggcaa cagctgcgtt
    19621 tgtatactgt acggccatca atgaaagcga tactcctatg aaaggtccaa atatagaacc
    19681 tgcaaaagtg gctttcattc ccttggaatt cataagtcca tccacaagat ttctgaacct
    19741 gcctgaaaga acaataatca gaaagaaacc tatcaaacct gtgattactc ttatttgagt
    19801 tgctgcaaat gggatataat gttctactgt agcaccttgt gcagatgctt ggtaataatt
    19861 catcccctgt ttgcttaaaa ctattccaag tccctgccct aaagctcccc caatacccaa
    19921 caggaaaccc ttcaagggca tgttaagttt gatgcgccca ttaactgtat tcttctttcc
    19981 gtccacagat gagctgctgt cccttttcaa aacggagatt gctattcctg tgagagttac
    20041 taacatgcca agaaaagcct gcaaagtcaa tcgctccccc agaataaacc agcctgacag
    20101 tgccgcaaaa ggaggagcaa gtgtcatgag aagttgtgaa aatctcgcag taataagtaa
    20161 ataagaatga aatagacaaa gatctccaaa tacaaatccc acaaggccag aaaggatcat
    20221 ccatatccaa gtgtctcgat ctgcatgtat cggcagtggt gaacctgtta aaatcaataa
    20281 cagccctccg agcagaaaaa aagcaaaaaa cagtctcagt atgttgacaa ttagagaacc
    20341 aatcttatta cctgcatatt cgaaaaagag agctgaaagg gtccaagaaa ccgctacgcc
    20401 tattgagata atttctccca agaatctcat ctactatcat tgtttttttg accgtcaaag
    20461 gtaacttttt tcgaattatt gaacctgtta gtctataatt acaggtgttt ggtcgataac
    20521 tttttaattc agaaggctat ggctataatt ttgcaaaaaa ttacgaaaaa actaagatgg
    20581 atagaaaggt tttactaact gtcattatta caactttaat atgtttttac aatgacagtt
    20641 attcgcaaga aaacaaaaaa tggacattgc gcgaatgcat cgactatgct ctctcaaaca
    20701 atgtaagtat caaaagcgca gaaataagcg ttaaaagcag tctggaagat ttgcaacagg
    20761 caaaggcttc aatgttacct tcactcagtt tatcatcctc acaaaatttt tcagcacaaa
    20821 aacaagaaag aacagatgag agttttgtta caaagggtag ttacacagga agctatgctc
    20881 taaacacgag tgtaacactc tacagtggtg gcaagat
//
LOCUS       DAOTQL010000015        18769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2908_length_18769_cov_5.245431, whole genome shotgun sequence.
ACCESSION   DAOTQL010000015 DAOTQL010000000
VERSION     DAOTQL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18769
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2908_length_18769_cov_5.245431"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5293_01835"
     CDS             complement(<1..605)
                     /locus_tag="P5293_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48707.1"
                     /translation="MKLNVENFKSVLQKATLNFSIESVQLKLTADKIQSRMLMQNSKD
                     AIVILDVPNDVFELKKVVDFTLNFSEPSISVMPYLTLIESDEEVDVRVFDEKITITSG
                     NQKSNIFFCSPNVVTTFSASAPRDNVNYFVSFDLNKSFVIDFNKIKKIGMRFGKIYFT
                     VINKVFAMETTDKLNPYSNTLKFDLLQNVKEDDLSLCFDYR"
     gene            complement(612..785)
                     /locus_tag="P5293_01840"
     CDS             complement(612..785)
                     /locus_tag="P5293_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48708.1"
                     /translation="MKELDKSKTDEEIVFQATTSSFTETTSAGKVEIKEKPEKKKTKK
                     TGRKRLTKKKKEE"
     gene            complement(872..1228)
                     /locus_tag="P5293_01845"
     CDS             complement(872..1228)
                     /locus_tag="P5293_01845"
                     /inference="COORDINATES: protein motif:HMM:NF028100.2"
                     /note="The founding member of this family is gp62 from
                     phage T4; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase clamp loader subunit A"
                     /protein_id="HRR48709.1"
                     /translation="MKEKTIFDILNSIYYKTGMDYEKKIVSAYVLSLWLSHDNSLIEI
                     VNDVNMVLFNLDDEWIYKYYYHKVPKGRRYIRWPRKSEKDKERGEKIKALMTEYGISK
                     REAEKCLVEYVRNARK"
     gene            complement(1234..2142)
                     /locus_tag="P5293_01850"
     CDS             complement(1234..2142)
                     /locus_tag="P5293_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR48710.1"
                     /translation="MENEKISNTDIWVFKHEPQKFGDMILNDVIRPRLQKAIIEIPNI
                     MLVGPPGVGKGTFTNIFLRETNLDHIKLNCSDETSIDSIRTKVKSFATALGRTKLKIV
                     VMNESDYLSPSAQAMLRDLIEQVSEITRFIFQCNYGHKMIKEIQSRCQVIELTNPPAK
                     EIYKHCMNILKKEKVKVIDNKQIISIIKQLYPDIRKIIHTLQLNTIDGKIDNIKADEV
                     SEVHAQIFKAACEKNVDEIRKLLRAHSINYPDLYLYFYEKAGTMKSPGDVILAVGEYL
                     YRDSLVANKEINFMTMVVSLIKRGIL"
     gene            complement(2279..2554)
                     /locus_tag="P5293_01855"
     CDS             complement(2279..2554)
                     /locus_tag="P5293_01855"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRR48711.1"
                     /translation="MNRKILSDFVSNDMSITKTDAKLAVDSVISGILDGLLRGEKISL
                     MGLGTFYTLNMPERNIRNPKTGEQITISPKKVIKFKASKLLKKELNK"
     gene            2906..2984
                     /locus_tag="P5293_01860"
     tRNA            2906..2984
                     /locus_tag="P5293_01860"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2940..2942,aa:Asn,seq:gtt)
     gene            3252..3327
                     /locus_tag="P5293_01865"
     tRNA            3252..3327
                     /locus_tag="P5293_01865"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3285..3287,aa:Pro,seq:tgg)
     gene            3475..3549
                     /locus_tag="P5293_01870"
     tRNA            3475..3549
                     /locus_tag="P5293_01870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3507..3509,aa:Val,seq:tac)
     gene            3552..3628
                     /locus_tag="P5293_01875"
     tRNA            3552..3628
                     /locus_tag="P5293_01875"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3586..3588,aa:Ala,seq:tgc)
     gene            3769..3855
                     /locus_tag="P5293_01880"
     tRNA            3769..3855
                     /locus_tag="P5293_01880"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3804..3806,aa:Tyr,seq:gta)
     gene            3945..4019
                     /locus_tag="P5293_01885"
     tRNA            3945..4019
                     /locus_tag="P5293_01885"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3977..3979,aa:His,seq:gtg)
     gene            4028..4106
                     /locus_tag="P5293_01890"
     tRNA            4028..4106
                     /locus_tag="P5293_01890"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4064..4066,aa:Asp,seq:gtc)
     gene            4109..4181
                     /locus_tag="P5293_01895"
     tRNA            4109..4181
                     /locus_tag="P5293_01895"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4142..4144,aa:Cys,seq:gca)
     gene            4338..4415
                     /locus_tag="P5293_01900"
     tRNA            4338..4415
                     /locus_tag="P5293_01900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4373..4375,aa:Glu,seq:ttc)
     gene            4550..4624
                     /locus_tag="P5293_01905"
     tRNA            4550..4624
                     /locus_tag="P5293_01905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4582..4584,aa:Lys,seq:ttt)
     gene            4734..4811
                     /locus_tag="P5293_01910"
     tRNA            4734..4811
                     /locus_tag="P5293_01910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4767..4769,aa:Lys,seq:ctt)
     gene            4821..4899
                     /locus_tag="P5293_01915"
     tRNA            4821..4899
                     /locus_tag="P5293_01915"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4855..4857,aa:Trp,seq:cca)
     gene            5007..5083
                     /locus_tag="P5293_01920"
     tRNA            5007..5083
                     /locus_tag="P5293_01920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5041..5043,aa:Glu,seq:ctc)
     gene            5093..5395
                     /locus_tag="P5293_01925"
     CDS             5093..5395
                     /locus_tag="P5293_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HRR48712.1"
                     /translation="MMYNDYYIYKDVRPNGEYIKIQVIRELRTVLSIGLKEAKNIADF
                     MDKKMSGGSSGHTYRLNMTSGQALQLVRLGFDVWHHLGGKVREENNHLPDELFVIE"
     gene            5396..5471
                     /locus_tag="P5293_01930"
     tRNA            5396..5471
                     /locus_tag="P5293_01930"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5429..5431,aa:Ala,seq:ggc)
     gene            5484..6092
                     /locus_tag="P5293_01935"
     CDS             5484..6092
                     /locus_tag="P5293_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48713.1"
                     /translation="MGKTFITADTHFGHEDIIGFCNRPFKNVKKMDEALIRNWNGRVK
                     AKDTVIFLGDFAFRECNKIDYYLDKLNGNKVFVRGNHDRNNGLKTKIENLVLRNGNDL
                     LFCVHDPRHINRNITINLVAHTHMNWKARRITWEDGHRGNIIINVGVDWWNFHPIELQ
                     EVMAYVNHIKKNFPVLNYNYGEWVKPFDDVCNGMIRNTHLIL"
     gene            6098..6337
                     /locus_tag="P5293_01940"
     CDS             6098..6337
                     /locus_tag="P5293_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48714.1"
                     /translation="MYEGKELLLTGHLTFPRPEADLLVKVRNGLWSYDELMEFVAEFD
                     LKFDEWCLLSRLPHSPDRETVNKLCMDVARKHLMR"
     gene            6526..6602
                     /locus_tag="P5293_01945"
     tRNA            6526..6602
                     /locus_tag="P5293_01945"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6560..6562,aa:Phe,seq:gaa)
     gene            6605..6680
                     /locus_tag="P5293_01950"
     tRNA            6605..6680
                     /locus_tag="P5293_01950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6638..6640,aa:Thr,seq:cgt)
     gene            6687..6762
                     /locus_tag="P5293_01955"
     tRNA            6687..6762
                     /locus_tag="P5293_01955"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6720..6722,aa:Thr,seq:tgt)
     gene            6814..6888
                     /locus_tag="P5293_01960"
     tRNA            6814..6888
                     /locus_tag="P5293_01960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6846..6848,aa:Gly,seq:gcc)
     gene            6895..6972
                     /locus_tag="P5293_01965"
     tRNA            6895..6972
                     /locus_tag="P5293_01965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6930..6932,aa:Leu,seq:tag)
     gene            7058..7474
                     /locus_tag="P5293_01970"
     CDS             7058..7474
                     /locus_tag="P5293_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR48715.1"
                     /translation="MNYLAPRSLFDNILFKDFFGDDFFCPLPSLKKIGYPVDIYETDD
                     NLVLEVAAVGLDKSDIKVEIKDDILSISYNKKEEESERNYAYKGITKKSFNLAWRISD
                     KFDLSNTSANLDRGLLKVIVPVAPERKPKEIEVTIS"
     gene            7503..8075
                     /locus_tag="P5293_01975"
     CDS             7503..8075
                     /locus_tag="P5293_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR48716.1"
                     /translation="METRKARKRRLEENFFQKYCNGRGIDIGCGNDPLLPNIDKWDFS
                     ISPDQDATYMKGIPDNTYDFVYSSHCLEDLSRPDIAIKNWWRILKNGGYLIIFVPHRD
                     LFELRKTLPSAGNVNHRWYFVIDKNEPPVTIGLIPFIVDNLTDYDIIYVKKCDDNYSY
                     YIKDEKYVIASGEFSIEAVIKKGNKTKYID"
     gene            8084..8167
                     /locus_tag="P5293_01980"
     tRNA            8084..8167
                     /locus_tag="P5293_01980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8119..8121,aa:Leu,seq:taa)
     gene            8580..8665
                     /locus_tag="P5293_01985"
     tRNA            8580..8665
                     /locus_tag="P5293_01985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8613..8615,aa:Leu,seq:gag)
     gene            8675..8749
                     /locus_tag="P5293_01990"
     tRNA            8675..8749
                     /locus_tag="P5293_01990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8707..8709,aa:Ile,seq:tat)
     gene            8765..9034
                     /locus_tag="P5293_01995"
     CDS             8765..9034
                     /locus_tag="P5293_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48717.1"
                     /translation="MKQLEQKNLDEIMAKMFEMAGATYDPSIVKKNRWYLKYRWTKAQ
                     EESFKKWLVSYLKKNKFCLNTKIAEKQADYFILWTGFPTSDFEKK"
     gene            9035..9108
                     /locus_tag="P5293_02000"
     tRNA            9035..9108
                     /locus_tag="P5293_02000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9067..9069,aa:Gly,seq:tcc)
     gene            9117..10034
                     /locus_tag="P5293_02005"
     CDS             9117..10034
                     /locus_tag="P5293_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRR48718.1"
                     /translation="MDDRVIKNLDEQTPVDTIHNVGDFDTVLFKRDDLFLPFEDTPRL
                     GGGKVRQAFSLFSSLSEKLKRYNGISSYVSVSSPQAMVVTRTAKEFGYKTFLVVGVNS
                     SVENIIEKYRPIRECYRIGAEIHNLAKIGYNTVIKNRAKKFLEENNLYLVNFGINVDE
                     NTEALVGSVADQVKNVPDNLDAVIVPVGSGIQFASIIAGIKKYNKNVDRIIGIQISGY
                     DRTKDINYILDKLNIKCDYEFYIDRTYPYTKHITTKITNTEDFSKSFEMNVVYESKAW
                     KALLRERYNLKRTDKILFWIVGNNNYLYI"
     gene            10036..10111
                     /locus_tag="P5293_02010"
     tRNA            10036..10111
                     /locus_tag="P5293_02010"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10069..10071,aa:Thr,seq:ggt)
     gene            10120..10195
                     /locus_tag="P5293_02015"
     tRNA            10120..10195
                     /locus_tag="P5293_02015"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10153..10155,aa:Ile,seq:gat)
     gene            10202..10282
                     /locus_tag="P5293_02020"
     tRNA            10202..10282
                     /locus_tag="P5293_02020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10239..10241,aa:Leu,seq:caa)
     gene            10362..10781
                     /locus_tag="P5293_02025"
     CDS             10362..10781
                     /locus_tag="P5293_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48719.1"
                     /translation="MKIGFTGAKQGMTKDQRATLIGVLVASAPEEFHHGDCVGADEQA
                     HSIATNLVENVVIHPPINDNLRAFCEAKTILPKRDYLDRNRDIVDACDLLVAAPRSRY
                     EIMRSGTWATIRYARKRNKKIFIIWPEGGYANENFRE"
     gene            10762..10980
                     /locus_tag="P5293_02030"
     CDS             10762..10980
                     /locus_tag="P5293_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48720.1"
                     /translation="MKILENKKAKISGKMNEEYLVVHIISDETGKNICDVKLSLQEVL
                     NLVEYAIKDIFKRLENNVKNKTEKRDVL"
     gene            10970..11773
                     /locus_tag="P5293_02035"
     CDS             10970..11773
                     /locus_tag="P5293_02035"
                     /inference="COORDINATES: protein motif:HMM:NF017843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HRR48721.1"
                     /translation="MYYNYKKGIINPFFRIKKEALKTESGIKIPNRWALLNAKTNSLL
                     GIMSADYEVVLNSQVAKLFDEALKDYEVDKIGDYLDNLQRRWKRRIILKGDKVTFEVD
                     KDDPVTIVVEIFNGYDVRSSFGYELLACRIICENELLMGRRNIFKESYSHFIDNPKKL
                     QFSIEMKFDYFKENVKIWQKWTKEKRTKDDFINFVNKREYLSEKIKEEVIEGYDLVMN
                     RFGVDSKWGHFNILAYLATHQTKARVGSYLFSNRYKLFERMIEEYYNEE"
     gene            11932..12006
                     /locus_tag="P5293_02040"
     tRNA            11932..12006
                     /locus_tag="P5293_02040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11965..11967,aa:Gln,seq:ctg)
     gene            12256..12903
                     /locus_tag="P5293_02045"
     CDS             12256..12903
                     /locus_tag="P5293_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48722.1"
                     /translation="MILRFSEKGSPYIELDDHNRFIFSRLTANENGIKYILYTNAPLN
                     GEMPVRARDNWFTTTYFKNVKNGKMPGAPATNLGIPHSFKSNIYWKNGTISVLRKMIA
                     KYLLPTGEFPESAFPALAEDIVKYNRLADKDKKKLVEYVRNEEKKEEEEEEIKKPEKK
                     IKKEKVKKEKKINKSEKKARKKKTKKVEKEKIKKVKAKTKKTKKIKKVARKKRRR"
     gene            12905..13420
                     /locus_tag="P5293_02050"
     CDS             12905..13420
                     /locus_tag="P5293_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRR48723.1"
                     /translation="MRRNPIIIFCVLIGIIFFLSCISDSGEARTRISKPRCGDRICNG
                     TETCKTCPKDCGICPPPAPYCGDGSCNGTETCKICQKDCGVCPPAPSLNIILTWNANA
                     PEENVINYKIYYGYQSGVYAGSYDVGVNLSLALTLEQQKVYYFSATAVNAVGESDHSN
                     EITYSTMYNYW"
     gene            13436..13627
                     /locus_tag="P5293_02055"
     CDS             13436..13627
                     /locus_tag="P5293_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48724.1"
                     /translation="MKKQRVLEYKYVGVVDRLKKDLSGREDVFCLYTMTDTFEQTKAL
                     DCRMFPFKRIAKVKIVEIK"
     gene            13678..13890
                     /locus_tag="P5293_02060"
     CDS             13678..13890
                     /locus_tag="P5293_02060"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HRR48725.1"
                     /translation="MMEKKPIIEKCIGCKKVTEDNFCMVYVNPEGRWSLGGCGISTHI
                     PKTTAFVAAKMMNLLKASKKKVKTKK"
     gene            13904..14071
                     /locus_tag="P5293_02065"
     CDS             13904..14071
                     /locus_tag="P5293_02065"
                     /inference="COORDINATES: protein motif:HMM:NF038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HRR48726.1"
                     /translation="MLKLPVVEKCGVCAKISDGFCTVYSNPAGKWSVGGCYIATHIKK
                     VVVEKKRAGNS"
     gene            14256..14915
                     /locus_tag="P5293_02070"
     CDS             14256..14915
                     /locus_tag="P5293_02070"
                     /inference="COORDINATES: protein motif:HMM:NF033518.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HRR48727.1"
                     /translation="MIIGENCLRRNWVTSKRLIGIAKAAELLGVGISTLRAWDDSGAL
                     KAERTKGGHRRYRIEDIEQLQGVVSEDNPPNDCTAVYCRVSSHDQKQKGDLERQKLRV
                     LEYCAGKQYRVGYVFEETLSGMNDNRPKLHKLINLAVEHKINRLVIEHKDRLARFNFG
                     IFVKLFESHGVAVEWCEEVLPKSYEAELVEDMLSLLSSFSAKIYGKRSAERRKKASGN
                     P"
     gene            14902..16029
                     /locus_tag="P5293_02075"
     CDS             14902..16029
                     /locus_tag="P5293_02075"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR48728.1"
                     /translation="MEILRAYKVELDPNNVQRTALLKHAGAARFAFNWGLARRKQEYE
                     ETGKSSNAIEQHRQLNALKPTEFPWMYEVSKCVMQEALRDLDKAYQNFFRRVKAGEKP
                     GFPKFKSRKSGVGSFRLTGAIRVENSRIRLPRIGWLRLKEHGYIPTEGIHILSATVTE
                     SAGRWFVSVQCKREIESAQAMGEPVGVDLGIKNLAVTSDGQRFENPKPLKKAQAKLRR
                     LQRELSRRKKGGKNREKTRQKIAKVHQRITNIRRDALHKVTSAIVAKTKPDNERPSVI
                     VLEDLNVSGMLANHCLAQAINDVGFAEFRRQVKYKSLWYGSELLLADRFFPSSRICRY
                     CGCINSELKLSDREWTCDCGAVLDRDLNAAINLKNLAINLP"
     gene            16216..16302
                     /locus_tag="P5293_02080"
     tRNA            16216..16302
                     /locus_tag="P5293_02080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16249..16251,aa:Ser,seq:tga)
     gene            16385..16479
                     /locus_tag="P5293_02085"
     tRNA            16385..16479
                     /locus_tag="P5293_02085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16421..16423,aa:Ser,seq:gct)
     gene            16593..16781
                     /locus_tag="P5293_02090"
     CDS             16593..16781
                     /locus_tag="P5293_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48729.1"
                     /translation="MTSKKEEKEVTVVRVTRKEFELSNGEIYEHPIELDETPTLSEFK
                     KYYHYWKKILSKDNIFED"
     gene            16782..17402
                     /locus_tag="P5293_02095"
     CDS             16782..17402
                     /locus_tag="P5293_02095"
                     /inference="COORDINATES: protein motif:HMM:NF033518.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HRR48730.1"
                     /translation="MEKLISISEASKLLNISKVSLRRYSEDYLKPVYTEGGHRRYKLS
                     DILKLQGVVEQEKEQSAAIYCRVSTSDQKNKGDLTRQKERLVEYCVQNKLKIEYIFEE
                     VGSGLNDKRKKLRELFELVSKNKISKVIIEHKDRLTRFNYNIFEFFINSYGVTIDCVE
                     SVLPKSFENELVTDILTIMSCYSAKIYGRRSHMTKKNKENEDKQSI"
     gene            17380..18630
                     /locus_tag="P5293_02100"
     CDS             17380..18630
                     /locus_tag="P5293_02100"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HRR48731.1"
                     /translation="MKINRAYKVELNPNNKQRTLLEKSVGCARFAYNWGLNERIELYK
                     SEKKTLTAIDQHKILCSIKKTDYPWMYEVSKMAPQEALRDLDTAFKNFFRGLKQGKKV
                     GFPKFKSKHNDKDSFRISYGFYVTNSTINIPKVGRVRLKEKGYIPTKDVKINSMTVSK
                     EADRWYISIQVEQDIPEPTKQIQSVLGVDVGIKTLATCSNGQIFENPKYLNKTKKKLA
                     HAQKNLARKKFDKETKRSSNNRLKSKLKVQKIYKRISNQRKDTLHKMTSILAKTKPRY
                     IVLEDLNVSGMMKNHKLAGAIGDASFNEIKRQLIYKTSWYGGSIIEVDRFFPSSKMCS
                     VCGYLKEDLTLEDRIYICDCGNVIDRDLNASINLEKYGLIKLNNTDSLSGIKACGESV
                     RLQDACIDQEAVSMKQEENIKCSI"
BASE COUNT         6136 a         3111 c         4064 g         5458 t
ORIGIN      
        1 tttctgtaat cgaaacagag gctcaaatca tcttctttta cattctgtaa cagatcaaac
       61 ttgagggtat ttgaatatgg attcaattta tctgttgttt ccatagcaaa cactttgttt
      121 ataacagtaa aataaatttt gccaaatctc ataccgattt ttttgatttt gttgaaatca
      181 attacaaatg atttattcaa atcaaatgat acaaaataat ttacattgtc tcttggggcg
      241 gatgctgaga aggtcgtaac cacatttggt gaacagaaaa agatatttga tttctgattt
      301 cctgatgtga ttgttatctt ctcatcgaaa actcttacat ccacttcctc atcgctttcg
      361 ataagggtaa gatatggcat tacagaaata ctgggttctg aaaagtttag ggtaaagtca
      421 actacctttt tcaattcaaa aacatcattc ggaacatcca gaattactat tgcgtccttg
      481 gaattctgca tcaacattct tgattgaatt ttatcggcag ttaatttcaa ctgcacactt
      541 tcaatcgaga aattgagtgt cgctttctgt aacacagatt taaaattctc aacatttaat
      601 ttcatattat atcactcctc ttttttcttt tttgtcaatc tttttctgcc agttttttta
      661 gtctttttct tctccggctt ttcctttatt tctacttttc cagcggaagt tgtttctgta
      721 aaggatgatg ttgttgcttg aaaaacaatt tcttcatcag ttttagattt atccaattct
      781 ttcaacaatt ttagtctctg ttccttgata gctttttccg tattgatatc aaatctacac
      841 ctgaaacaga taccatcata caatcttatt cttactttct ggcatttctt acatactcca
      901 ccaagcattt ctctgcctct ctctttgata tgccgtattc ggtcatcaaa gcttttattt
      961 tttcccccct ctccttatcc ttttcggatt ttcgcggcca ccttatatat cttcgtcctt
     1021 tgggaacttt atgataatag tatttatata tccattcatc atccagattg aataaaacca
     1081 tattcacatc attgactatt tctataagag aattatcgtg agatagccac aatgaaagga
     1141 cataggctga aacaattttc ttctcatagt ccatacccgt cttataatat atggaattga
     1201 gaatatcgaa tattgttttc tctttcatag tatttataat atcccccttt ttataaggga
     1261 gacaaccatc gtcataaagt ttatttcctt atttgccaca agactatctc tataaaggta
     1321 ttctcctact gctaagatga catcgccggg gcttttcatt gtaccggcct tttcatagaa
     1381 ataaagatat aaatctggat agtttataga gtgtgctctc aagagcttcc tgatttcatc
     1441 cacatttttc tcacaagctg ccttgaaaat ctgtgcgtga acttctgata cttcatccgc
     1501 cttgatgtta tctattttac catcaatagt gttcaattgt aaagtatgga tgattttcct
     1561 gatatcggga taaagctgtt tgatgatgga aataatctgt ttattgtcta tcaccttcac
     1621 cttttccttt tttaggatgt tcatacaatg tttataaatt tcttttgcag gaggattggt
     1681 caattcaatg acttggcatc ttgattgaat ttcttttatc atcttgtgtc cataattgca
     1741 ctggaagatg aatcttgtga tttcactaac ttgttcaatc aaatctctca acatcgcctg
     1801 ggctgatggt gaaagataat cactctcatt cattaccact atttttagtt ttgtacggcc
     1861 aagagctgtg gcgaatgatt ttacttttgt tctgatgctg tcaatagatg tttcatccga
     1921 acaattcaac ttaatatggt caagatttgt ttccctcaaa aagatattcg tgaatgttcc
     1981 cttacctaca cccggcggcc ccactagcat gatattcggt atttcgatta ttgctttctg
     2041 taatcttggt ctgattacat cattcaatat catatccccg aatttttggg gttcgtgttt
     2101 aaacacccat atatcagtat tacttatttt ttcattctcc attttctcat tccttctttt
     2161 gtgtctctac tttttttccc tctttcttca gttgtataaa tgtgtccacc ttttttctat
     2221 ggattttgtt gtttgtccaa tataagtgat aataccataa atcttcagat tatccatatt
     2281 atttgttcag ttctttcttg agtagttttg atgccttgaa tttgataacc ttctttggtg
     2341 atatggtaat ttgttcgccg gtctttgggt tcctgatatt tctctcgggc atattcaatg
     2401 tataaaatgt gccgagaccc atcagggaaa ttttttctcc tcttaaaagg ccgtcgagaa
     2461 taccagaaat aacagaatca actgcaagtt tagcatccgt tttggtaata ctcatatcat
     2521 ttgatacaaa atcagataaa attttcctgt tcatacgaaa tttctccttt tttagatttt
     2581 aatttattat atcagaaaaa tatcaaaatg taaacttctc cgatattttt catatgatat
     2641 aatataacac attattttca ctatataaat atttttgttg atattgaaat aaatatacgg
     2701 taagatatta ttgttctttt gaaatttggg gatgaaattg ggttcgacag aaatttttat
     2761 atatccggtg catgccggag atggttatta ggctccgtta tcaatacaat gtaagtattg
     2821 gaaattattg agaaagattt ttgttgacag gacatactaa ttacggtata atatgtcctc
     2881 tttgaatgta agaaattaca aaaattgcgg agtgaaggcg aatggtaagc cggcgccctg
     2941 ttaaggcgta gattgtgcag gttcaagtcc tgtctccgca gccagaaaaa aaaatgttta
     3001 catttattgt aaactatgat ataatttcct aaataataag agattctact ttttaggggt
     3061 cgttatgagt aaacatcagt tacatacata ttgttggttt tatcaggtgc cgacccttgc
     3121 gattgaaacg aagccagcag agggggcgct tatcgaataa aaggtcagaa tcctataaag
     3181 tgaatttatt gaagggagat tgggttttga cctaatctcc cttttttgtt tttgttcatt
     3241 gaaaaatatt tcggagagtg gcgcagatgg tagcgtgctt gacctgggat caagaagtcg
     3301 caggttcaaa tcctgtctct ccgaccaaaa aataaatggt gtaaacaagg tagtccggaa
     3361 cgccttggtc gaatcgtcta atggatagga caactttgag gaagtaaatc tgtagcgcta
     3421 cggaaacttc cataaaggaa atgtgggttc gactcccact cgataacacc ttatggccgg
     3481 ttagcttagt gggaaagccg ctgttttaca cgcagcttag agaaagttcg attctttcac
     3541 cggccaccaa tggggctgta gccaaacttg ggaaggcact acatttgcaa tgtagtcatt
     3601 gagagttcga atctctccag ctccaccaga aaggggaagg atatggattc aattcctatt
     3661 cccgatgaag gtatatgtaa acgatgttaa atgctagctt atgacataga ggaacatata
     3721 ttcagagatc tcgtataatg gatattacat cctcagcaaa aattatgtgg ggagttaaac
     3781 cgtaagtagt agcgggccgg actgtaaatc cggtgtcttc gggctcgggt ggtgcaactc
     3841 catcactccc caccaaaata agtagaagta gaaaagtatt ggttggatac agttaccgaa
     3901 gacccaacta tcacatggta acctactcct accatgagaa tatggtggtc gtggccaaat
     3961 gggaaggcaa gagattgtgg ccctctcaag agtgagttcg atcctcaccg atcaccccaa
     4021 ataaaatggt gggttagctt agtctggttg aaagtgctgg cctgtcaagc cagagatcac
     4081 gggttcaaat cccgtactca ccgccaatga gctcgtagcc aaacagggaa ggcaacggtc
     4141 tgcaaaaccg taaattgtgg gttcaaatcc catcgagctc tctaaaaaat atgggagatg
     4201 tgggttaaag ccccaccgga ttctgcagta atccgtaggt taatgagaat aaacacgggg
     4261 aaagaccgtg aacatactat cataagtctg taaaaatcac gggtacagac ctgatagttc
     4321 tttcccccaa aaaatacaga cctgtgggcg aaaggaatta agccgtcacc ctttcaaggt
     4381 gaaaattgcc ggttcaagtc cggtcaggtc taccagatat gggatgtggg cttatcgttg
     4441 aaatatacgg gttggacatg agcctgtaca ggatcgggag tttatggggt gccaactttt
     4501 acctacagcg gcccgatagt gggttaaatc cccaccacat cccacgaatg agaccgtcgc
     4561 tcaatggcag agcaccagac ttttaatctg ggctatgtgg gttcgattcc catcggtctc
     4621 accagaatat aagttgattt attcgatatc gctcgaggtc gcaagtgttg agaggttttt
     4681 aaccataaaa gaacgacaca taaaggaagg ataaatcaac ctactggaat aggggagttg
     4741 tcgcctaatt ggcatggcac ctgactctta atcaggacaa attgtgggtt caaatcccat
     4801 caactccacc aaaataaaat ggggtggtcg agcaggtggt gagctcagca gtctccaaaa
     4861 ctgtataaag tggaggttcg attcctcccc gccccgccaa ttatgtttat atcaaaggca
     4921 accgtatgtg ccgattagtg gtctgaagct gagccttgtg gttcggtgga cggttcaatt
     4981 ccgttgatat aaacattata atttttgctc ccatcgacta atcggttagg tcgccaccct
     5041 ctcaaggtgg agcttcgggg ttcgactccc cgtgggagta ccaaaggaaa aaatgatgta
     5101 taatgattat tacatttata aagatgtaag accgaatggt gagtatataa aaatacaggt
     5161 catcagggaa ttgaggacgg ttctttctat cggtctgaaa gaggcaaaaa atattgctga
     5221 ttttatggat aagaaaatga gtgggggatc ttccggacat acatatagat tgaatatgac
     5281 aagtgggcag gcattacaat tggtgagatt gggttttgat gtctggcatc atcttggtgg
     5341 aaaagtaaga gaggaaaaca atcatctacc ggatgaatta tttgtaattg aataaggggc
     5401 tgtagctcaa ttgggagagc gcttgaatgg cattcaagag gtagcgggtt cgaatcccac
     5461 cagctccacc aaaggatata aaaatgggaa aaacatttat aacagcagat actcacttcg
     5521 gtcacgaaga tataatcggt ttctgcaata ggcctttcaa gaatgtaaag aaaatggacg
     5581 aggccttgat aagaaactgg aatggaagag tgaaggcaaa agatacagtt atattccttg
     5641 gagattttgc cttcagggaa tgtaacaaga ttgattatta tcttgacaag ttgaatggaa
     5701 ataaagtatt cgttcgcggc aaccatgacc gtaacaatgg tctcaaaaca aaaattgaaa
     5761 accttgtttt gagaaacggt aatgatcttt tgttttgtgt tcacgatcca aggcatataa
     5821 atagaaatat caccatcaac cttgttgcac atactcatat gaactggaaa gcaagaagga
     5881 ttacatggga agatggtcat aggggaaaca ttattatcaa tgttggggta gattggtgga
     5941 attttcatcc aattgaatta caggaagtaa tggcttatgt aaatcatata aagaaaaact
     6001 ttcctgtatt gaattacaat tacggagaat gggtaaaacc ttttgatgat gtctgtaacg
     6061 gtatgatacg aaacacgcat ctcatcttgt aaggttgatg tatgaaggta aggaactgtt
     6121 acttaccgga catcttacct ttccaagacc ggaagcagac ttacttgtaa aagtgagaaa
     6181 cggtttgtgg tcttacgatg agttgatgga atttgttgcg gagtttgact tgaagtttga
     6241 tgagtggtgt ttgttatctc gacttcccca ttctcccgat agggaaacgg tgaacaaact
     6301 ctgtatggat gttgctcgta aacatttgat gagataacgg gttggatgcg aaagcaagct
     6361 gaccattcct ataagcgcct acaaggaatg gggagatccg tcaggaggca tttgccaccg
     6421 tggacgggaa cccataaaaa ttagggtgcg gtttaaaccg taggcatggg atgccgcaaa
     6481 ggcgatgagg ttatgataaa gttggatgcg gtctacccca acaatggaga tgtagctcag
     6541 taggtagtag cgcttgcctg aagagcaaga ggtcgccggt tcaattccgg ctgtctccac
     6601 caatgccgga atggcgcaat tggaaacgca ggactttcgt aaggtcaaga ttatcggttc
     6661 gaatccgatt tccggctcca ataaatgcca atgtagttta atcggtaaaa tacaactctt
     6721 gtaaagttgg tgatggtggt tcgaatccac tcattggctc catttatatt gttcaaatga
     6781 agatagattt agtggtaaaa gtttttaaaa taagcgggtg taactcagtg gtagagtgct
     6841 accttgccaa ggtagacgtc gacggttcaa atccgttcat ccgctccaaa ttgtgccggt
     6901 gtgatggaat ctggcataca tactgcgctt agaacgcagg ttttcggggt tcaaatccct
     6961 gcaccggcac cataaataga tatggattca caggcggaaa ttttaattgg ggtatcttag
     7021 gagccccaaa ataaaccatc ttaggaggtt actgactatg aattatttag caccaagaag
     7081 tttatttgat aacatacttt tcaaagattt ttttggggat gattttttct gcccccttcc
     7141 gtctttgaaa aaaattggtt atccggttga tatctatgag accgacgata accttgtcct
     7201 tgaggttgcc gcagttggac ttgacaagtc tgatatcaag gtcgagatta aggatgatat
     7261 tctttcgatc tcttataata agaaggaaga agagtccgaa agaaactatg cctataaagg
     7321 cattacgaag aagtccttca atcttgcttg gagaatttca gacaaatttg atttgtcgaa
     7381 tacatccgcc aatcttgata gagggctttt gaaggttatt gttcctgtgg ctcccgaaag
     7441 gaaaccaaag gaaattgaag tgacaattag ctaatttttt ccgcctgtga atccatcatt
     7501 ttatggaaac aagaaaagca agaaaaagaa gactcgaaga gaattttttc caaaagtatt
     7561 gtaatggaag gggtattgat ataggttgtg gtaatgatcc tctgttacct aatattgaca
     7621 agtgggattt ttccataagt ccggatcaag atgcaaccta tatgaaaggt attcctgaca
     7681 acacatacga ctttgtatat tcgtcacatt gtcttgagga tttatcacga cccgatattg
     7741 caataaaaaa ctggtggaga attttgaaga atggtggcta cctgattata tttgttcctc
     7801 accgtgatct gtttgagttg agaaaaacat taccctctgc cggtaatgta aatcataggt
     7861 ggtactttgt cattgacaag aatgagccac cagttacaat aggattgata ccgtttattg
     7921 ttgacaatct tacggactat gatataattt atgtgaagaa gtgtgacgat aactacagtt
     7981 attatataaa ggacgaaaaa tatgtaatag caagtggaga gttttccatc gaggctgtga
     8041 taaaaaaggg aaacaaaaca aaatatattg attgagccga attgcctgag tggtggaatt
     8101 tggtagacac aataggttta aaccctatcg ggaaaccatg caggttcaaa tcctgtctcg
     8161 ggcaccataa aatttaagaa aaaagagtag agataaacag aaaggttagt ataaaacgaa
     8221 agggtgttcc aactaaaggg aaatggtgtt ttacggatga acagcgcaga aaaggtggaa
     8281 cgattagtgg aaagataaga gccaaaaggg ttcttagtga gatattatca cttcctttta
     8341 atgaattgtc taacaaatac agtagaacag gaataagaac aagggttttg tatgaacaaa
     8401 accacaaatg taataaatgt tctttaaaaa gttggttggg taaaccttta acattggaac
     8461 ttcatcatat tgatggtaat aaaacaaata ataaaagaga aaatttagaa gctatttgtc
     8521 ctaattgtca tagtcaaaca gacaatttta gatttaaagg aaagacacat tcaaagaatg
     8581 gggaagtggt gtaatagcag ccacgcaagt ctgaggggct tgtgctcgaa agggcgtgta
     8641 ggtgcaaatc ctaccttcct caccaaataa aaatggccgg gtagttcaat ggtagaacgc
     8701 gcgacttata atcgcctgat ggtaggttcg actcctcctc cggctaccag aataagaggt
     8761 tattatgaaa cagttggaac agaaaaatct cgatgagata atggcaaaga tgtttgagat
     8821 ggccggcgcc acttatgatc cgtctatcgt gaagaaaaat agatggtatc tgaaatatag
     8881 gtggacaaag gcacaggaag aaagtttcaa aaaatggttg gtatcctatt tgaagaaaaa
     8941 taaattttgc ctgaatacga agattgcaga gaagcaggct gattatttta ttctctggac
     9001 aggatttcca acatcggatt ttgagaagaa ataagcgggg ttcgtatagt ggtaatacct
     9061 tagccttcca agctaaagcc gagggttcga ttcccttact ccgctccaaa atttttatgg
     9121 acgatagagt tataaaaaat cttgatgaac aaactccggt ggatactatt cacaatgtcg
     9181 gagattttga tactgttctt ttcaagagag atgatttgtt ccttccgttt gaggacactc
     9241 cgcgattggg tggggggaaa gtgagacaag ccttttctct gttttcttct ctgtcggaaa
     9301 aattgaaaag atataatggt atctcaagtt acgtttccgt ttcctcaccc caggcgatgg
     9361 ttgtgacaag aacggccaag gagtttggtt ataaaacatt ccttgttgtt ggtgtgaact
     9421 cgtctgttga aaatataatt gagaaatatc ggccaatcag ggaatgttac aggattggtg
     9481 cggaaattca taacttggca aagataggtt acaatacagt aatcaaaaat agggcaaaga
     9541 aatttttaga ggaaaacaat ctgtaccttg tcaactttgg tatcaatgtt gatgagaata
     9601 ccgaggcgct tgtgggatct gtggcggatc aggtcaaaaa tgtacctgac aatcttgatg
     9661 ccgttatcgt tccggtgggg agcggtattc aattcgcctc aattattgcc ggtataaaga
     9721 agtataataa aaatgttgat agaattattg gtatacagat ttccggatat gatcggacaa
     9781 aggatatcaa ttacatactg gacaaattga atataaaatg tgactatgaa ttttatattg
     9841 ataggactta tccgtacaca aaacatataa caactaaaat aacgaatact gaagatttta
     9901 gtaagagttt cgaaatgaat gttgtatatg aatcgaaggc gtggaaagcc ctcttgagag
     9961 aaagatataa tctgaaaaga acagataaaa ttttattctg gatagtaggt aataacaatt
    10021 acctttatat ataatgctat cgtggctcag taggcagagc atatccttgg taaggataag
    10081 gtcggcggtt cgattccgcc cggtagctcc agaaaatata gccgggaagc tcagcaggca
    10141 gagcactcga ccgataatcg agcggtcgcg ggttcgactc ccgctccggc taccatatta
    10201 tggcggtgtg acgggaatct tggcatacct tggaagctca aaccttccag ttctgtgagt
    10261 tcgactctca ccaccgccac cagataacta cccgtttttc ttatgttact tttctattta
    10321 caagttactt gaaatatggt aagatataac agaggtgaaa tatgaaaata ggttttacag
    10381 gagcgaaaca agggatgacc aaggatcaaa gggctaccct gattggtgtt cttgtggctt
    10441 ctgctccaga ggaatttcat cacggtgatt gtgtgggggc tgatgaacag gcacattcaa
    10501 tagcaaccaa tcttgttgaa aatgtagtta tacatccacc aatcaacgat aatcttcggg
    10561 cattttgtga agccaagaca attttaccga aaagagatta ccttgataga aatcgcgata
    10621 ttgttgatgc ctgtgattta cttgtggcgg caccacgctc aagatatgaa ataatgagaa
    10681 gtggaacatg ggccactatc aggtatgcaa gaaaaagaaa taaaaagatc tttataatct
    10741 ggccagaagg gggttatgct aatgaaaatt ttagagaata aaaaggcgaa gatttctgga
    10801 aaaatgaatg aggaatatct tgttgttcat atcatttcag atgaaaccgg taaaaatatt
    10861 tgtgatgtga aattatcttt acaggaggtt ctcaaccttg tagagtatgc aataaaagat
    10921 attttcaaac gtcttgaaaa taatgtcaag aataaaacgg agaaaagaga tgtattataa
    10981 ttataaaaag ggtataataa atccattttt cagaataaag aaggaagctc ttaaaacaga
    11041 gagtggtata aaaattccaa atcgctgggc tctcctgaat gccaagacaa attcactttt
    11101 aggaataatg tcagcagact atgaggtagt tctcaattca caagtagcaa aactttttga
    11161 tgaggcatta aaagattatg aagttgacaa gattggggat tatcttgata atttacagag
    11221 aagatggaaa agaagaatta tcctcaaagg tgataaggtt acatttgaag tggataaaga
    11281 tgatcctgta acaatcgtcg ttgaaatttt caatggatat gatgtaaggt cgagttttgg
    11341 atacgagctt cttgcttgtc gaattatctg tgaaaacgaa ttgttgatgg gtcgaagaaa
    11401 tatcttcaag gaaagctatt ctcattttat agataatcca aagaaacttc aattctctat
    11461 cgagatgaaa tttgattatt tcaaagaaaa tgtaaaaatc tggcagaaat ggacaaaaga
    11521 aaaaagaacg aaggatgatt ttataaactt tgtaaataag agggaatatc tttcggagaa
    11581 aatcaaagag gaagtaatag aaggatatga cttggtaatg aacaggttcg gtgtggatag
    11641 caaatggggg catttcaaca tcctcgctta tcttgctaca catcagacaa aggcaagagt
    11701 tggttcatac cttttctcaa ataggtataa actctttgaa agaatgatag aggaatatta
    11761 taacgaagaa tgaaaaagat attacaattg ataatggtaa tgaaagtgcc ggccggcaca
    11821 ttctctatga gaggagtaag ataaagaaaa attcggggat cgtttaattg gtaagactca
    11881 acactttgaa tgttgtaata gaggatcgtg gcctcttccc cgaaccaata tacacgggtc
    11941 atctaattgg taggatatct ggctctgaac cagaaaatgc aggatcgtag cctgccccgt
    12001 gttccaaaaa tgagtagtgg atcaatgagg gtagtcattc ttcgcactca ccgttaagca
    12061 catctcatcg agtctgcagt aatcgatgaa aggcctatgt cgggaattgc catgggaatg
    12121 acgagcgacc tgatatccac tactcaatta tgggggttat tttacagtaa ccccctgttt
    12181 tcatttggta aaatagtcct ttacaggtaa cataaattgt gatataatat aggatactat
    12241 attaaggaga ataatatgat attgagattt tctgaaaagg gctcaccgta tattgaattg
    12301 gatgatcata ataggttcat cttttccaga cttacagcga atgagaatgg tataaagtat
    12361 atcctttata ccaatgctcc tttgaatggt gagatgccgg taagggcgag agacaattgg
    12421 tttaccacga catatttcaa gaatgtgaaa aatggaaaga tgcctggggc cccagcaacc
    12481 aatcttggta ttcctcattc tttcaaatcc aatatttatt ggaagaatgg aacaatctct
    12541 gttttgagaa aaatgatcgc aaaatatctt ttacctacag gagagtttcc tgaaagtgca
    12601 tttccggctc ttgccgaaga tatcgtgaag tataataggt tggcagataa ggataaaaag
    12661 aaactcgttg aatatgtaag gaacgaggaa aagaaagagg aagaggaaga agaaataaag
    12721 aaaccagaga agaagatcaa aaaagaaaag gtgaagaagg aaaagaaaat aaataaatcc
    12781 gaaaaaaagg caaggaagaa gaaaaccaag aaagttgaaa aggaaaagat caaaaaggta
    12841 aaagcgaaga ccaagaaaac caagaagatc aagaaagtgg caaggaaaaa aagaaggaga
    12901 taatatgaga aggaatccca taattatttt ttgtgtattg ataggtatta tttttttctt
    12961 atcctgtata agcgattctg gagaagccag aaccagaata tcgaaaccaa gatgtggtga
    13021 caggatatgc aatggaacag agacttgtaa aacttgtcca aaagattgtg gaatatgtcc
    13081 accacctgca ccatattgtg gagatggtag ttgtaatgga acagagactt gtaaaatctg
    13141 tcaaaaagat tgtggagtgt gtccacctgc gccctctctc aatataatat taacatggaa
    13201 tgcaaatgca ccggaagaaa atgtcataaa ttataaaatt tattatggat atcagtctgg
    13261 tgtatatgct ggttcctatg atgttggtgt taatttgtca cttgctctga cattggaaca
    13321 acagaaggtg tattattttt cagctacagc agtaaatgcc gtaggtgaaa gtgatcattc
    13381 aaatgagatt acttattcaa caatgtataa ttactggtaa tagaaaggag tagagatgaa
    13441 aaaacagaga gttttagagt ataagtatgt tggtgttgtt gatcgtttga aaaaggactt
    13501 gtcaggtaga gaagatgttt tttgtctcta tacaatgaca gacacatttg agcagacaaa
    13561 agctcttgat tgtagaatgt ttccattcaa gagaattgct aaggtaaaaa ttgtagaaat
    13621 aaaataattt attttctacc tacataataa atattataaa actctatgta ggtggaaatg
    13681 atggaaaaga aaccaattat tgagaagtgt atagggtgta aaaaggtaac agaggataac
    13741 ttttgtatgg tatatgtaaa tcctgaaggc cgatggtctt taggtggttg tggaatatca
    13801 acccatatac caaaaacaac agccttcgtt gccgccaaga tgatgaacct gttgaaggcg
    13861 tcaaagaaaa aagtaaaaac caaaaaatag aaaggaaagt aagatgttga aattacctgt
    13921 agtagaaaag tgtggagtgt gtgcaaaaat ctctgatggt ttttgtactg tttattctaa
    13981 cccagctgga aaatggtctg taggtggatg ctacattgct actcatatca agaaggttgt
    14041 tgtagaaaag aaaagggcag gcaacagttg atggataacc tactataacc tacttgacac
    14101 taccgaaatc ttttggtata attagaggtg aaggatgaaa agagcgatgg tcatacgagt
    14161 aaccaagact gaatttgaac ttgacgacgg gcggatttac cagcatccgt ttgaactcga
    14221 tgaagttcct accaacgagg aatttcagcg catctatgat cattggcgag aactgtttga
    14281 gaaggaactg ggtgacgagc aagagactga tcgggatagc gaaagccgct gaacttcttg
    14341 gcgttggaat ctctacactt cgggcgtggg atgattctgg cgcattgaaa gcagaacgca
    14401 caaagggtgg tcatagacga taccgcattg aagacattga gcaacttcaa ggtgtggtgt
    14461 cagaggacaa cccgcccaat gattgcacag cggtctattg tcgggtgtcc tcgcacgacc
    14521 agaaacagaa aggcgatctg gaaagacaaa agctgcgcgt gcttgaatac tgcgccggta
    14581 aacaatatcg agttggctat gtgttcgaag aaacgctgtc tggcatgaat gacaaccgcc
    14641 cgaaactaca caagctaata aacttggctg ttgaacataa gatcaatcgc ttggttatag
    14701 agcataagga tcgacttgcg cgtttcaatt tcggtatctt cgtcaaactg ttcgaaagtc
    14761 atggagttgc tgttgagtgg tgtgaggagg ttttaccaaa atcctacgaa gcggaattgg
    14821 tagaggatat gctgtctttg ctttcgtcat tctcagccaa gatttacgga aagcgttcag
    14881 ccgagcgaag gaaaaaggca agtggaaatc cttagagcat ataaggtcga acttgaccca
    14941 aacaatgttc agcggactgc attgctgaaa catgcgggcg cggcgagatt tgcgttcaac
    15001 tggggacttg cacggcgaaa gcaagaatat gaagaaacgg gcaaatctag caatgctata
    15061 gagcagcatc ggcaattaaa cgccctaaag cccacagaat ttccctggat gtacgaggtt
    15121 tccaaatgtg tcatgcaaga ggcgcttcga gacctagata aagcctatca gaactttttt
    15181 agacgtgtga aggctggtga gaagcctgga ttccctaaat tcaaatctcg caagtccggt
    15241 gttggcagtt tcagattgac tggcgctatc cgtgttgaga acagtagaat caggcttcct
    15301 cgcattgggt ggttacgact gaaagagcat ggctacattc ccacagaagg catccatatc
    15361 ttatccgcta ccgtcaccga gtcggcgggt cgttggtttg ttagtgttca atgtaagcga
    15421 gagatagaat ccgcacaagc aatgggcgag cctgtgggcg ttgacttggg tatcaaaaat
    15481 ttggctgtga cttcggatgg gcaacgattc gagaacccaa agccactaaa gaaggcacag
    15541 gctaaactca gacgacttca gcgcgaacta tcacgccgaa agaaaggtgg aaaaaatcgg
    15601 gaaaagacca gacagaaaat tgccaaagtg caccaacgga ttaccaacat ccgacgtgat
    15661 gctttgcaca aggtcacatc ggctatcgtg gcgaaaacca agcctgataa cgaacgaccc
    15721 agtgtaatcg tattggaaga ccttaatgtg tccggcatgt tggcgaacca ctgtttagca
    15781 caggcgatca acgatgttgg ttttgcggag tttcgaaggc aagtaaagta caaaagcctt
    15841 tggtatggtt cagagttgct attagccgac aggttctttc cgtcgtcgcg tatatgccga
    15901 tattgtggtt gtatcaattc ggaattgaaa ctatcagata gagagtggac ttgtgattgt
    15961 ggcgctgtct tggacagaga cttaaacgca gcaatcaatt tgaaaaatct agcaataaac
    16021 ctaccgtgag ttccacggga agttacgcct gcggagaccg tgtaagaccc gacttgtcgg
    16081 gcggtggtcg tggaggcagg aatcagacat cgaatctatc gttggtagat tcgagtaagt
    16141 tctgaggaac ggtcaatcca ttgaaggctt ccaagagggg tgagtaattt cgtagagccc
    16201 ttggaataag ataatggaga ggtggacatt tggaacgtca agtggtcttg aaaaccatca
    16261 cccgtaaaag ggctgcaggt tcgaatcctg tcctctccgc cattctttta taagaaaagc
    16321 ggacattgaa tatattttga aatgggcaaa aaagataaag gctgtgaaat accttagtgg
    16381 taatggtatg gtggccgagt ctggtttaag gcgctcgcct gctaagtgag tggacatgga
    16441 aatcatgtct cgcaggttca aatcctgtcc ataccgccag agaaagggga atctaacagt
    16501 tacgaaaaat cttatcaaaa atgaaaaatc tttatcaaaa tgaacacttt ttaataatca
    16561 attaatataa atacatagta ggaaggtaag gtatgacttc taaaaaggaa gaaaaagaag
    16621 tgactgttgt aagagtgaca aggaaagagt ttgaactaag taatggtgaa atttatgaac
    16681 atcctattga gctggatgaa actccaactc tttcagagtt taagaagtat taccattact
    16741 ggaagaagat tctttccaaa gataatattt ttgaggatta aatggaaaaa cttatatcta
    16801 taagcgaagc ttctaagctt cttaatatat ctaaagtatc tctaagaagg tatagcgaag
    16861 actatcttaa acctgtctat actgaaggtg gtcatcgtag atataagtta tcagacatac
    16921 ttaaacttca aggtgtagta gagcaagaaa aagaacagtc tgcagctatt tactgtagag
    16981 tttctacatc agatcaaaag aataaaggtg atttaacacg gcaaaaggaa aggcttgttg
    17041 aatactgcgt tcaaaataaa ttaaagatag agtacatttt tgaagaagtc ggttcaggtc
    17101 ttaatgataa aagaaaaaaa ctaagagagt tatttgaatt agtttctaaa aataaaataa
    17161 gcaaagttat tatagagcat aaagatagat tgacaagatt taattataac atttttgaat
    17221 tttttattaa tagctatgga gttactatag attgtgtaga atctgtgtta cctaagtctt
    17281 ttgaaaatga attagtaaca gacatcttaa ctattatgtc ttgttattca gctaaaattt
    17341 atggtagaag aagtcatatg actaagaaga ataaagaaaa tgaagataaa cagagcatat
    17401 aaggtagaat tgaatccgaa taacaaacaa cgaactcttc ttgaaaagag tgtcggatgt
    17461 gctcggtttg cttataattg gggattgaat gaacgtatag aactttataa gtcggaaaag
    17521 aaaactttaa ctgctataga tcaacataag atactttgtt ctataaagaa aacagattat
    17581 ccttggatgt atgaagtgtc aaaaatggca ccacaagagg ctttaagaga tttagatact
    17641 gcctttaaga actttttcag aggtttaaaa caaggtaaaa aagtgggttt tcctaaattt
    17701 aaaagtaaac ataatgataa agattctttt agaatttctt atggattcta tgtaactaat
    17761 tcgacaatta atattcctaa agtcggtaga gtaagactga aagaaaaagg ttacatccct
    17821 acaaaagatg ttaaaataaa ctctatgact gtatctaaag aagcagatag atggtatata
    17881 tccattcaag ttgaacaaga tatacctgaa cctacgaaac aaatacaatc ggtcttagga
    17941 gtagacgtag gcattaaaac attagctaca tgttctaatg gtcagatatt tgaaaatcct
    18001 aaatatctga ataaaacgaa gaaaaaatta gcacatgctc aaaagaactt ggctcgtaaa
    18061 aagtttgata aagaaactaa aagatcaagt aataataggt taaaaagtaa attaaaagta
    18121 caaaagatat ataaaagaat aagtaatcaa agaaaagata ctcttcataa aatgacaagt
    18181 attttggcga aaaccaaacc aagatacatc gttttagaag atttaaatgt ctctggtatg
    18241 atgaagaacc ataaacttgc aggagctata ggagatgcgt cttttaatga aattaaaagg
    18301 caactaatat ataagacttc atggtatggt ggttctataa tcgaagtaga tagattcttt
    18361 ccttcaagta agatgtgttc tgtctgtggt tatttaaaag aagatcttac tttagaagat
    18421 aggatttata tatgtgattg tggaaatgtc atagatagag acttaaatgc ttctattaac
    18481 ttagaaaagt atggattaat taaacttaac aataccgata gtttgtcggg aattaaagcc
    18541 tgtggagaaa gtgtaagact tcaagatgct tgtatagatc aagaagcagt ttctatgaag
    18601 caggaagaaa acatcaaatg ttcaatttga gtaagttttt cagaacggat atatgaaaga
    18661 tagattgagg gatttatact acagtattat tcttcttggg atgaacatag tctggctttc
    18721 tccgtggggt agagccatca ttcataaaac cgtgaatctc tatctaaat
//
LOCUS       DAOTQL010000016        18727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_2921_length_18727_cov_5.060358, whole genome shotgun sequence.
ACCESSION   DAOTQL010000016 DAOTQL010000000
VERSION     DAOTQL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_18727_cov_5.060358"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /locus_tag="P5293_02105"
     CDS             <1..1968
                     /locus_tag="P5293_02105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRR48732.1"
                     /translation="DAIRGFVEEREGIAHFLNSSLTKSQLQEVKDDLLAGNTKLLYVA
                     PESLTKEENIQLLKQIKISFYAVDEAHCISEWGHDFRPEYRRIHQIATDIGIAPIIAL
                     TATATPKVQHDIQKNLDMLEAKVFKSSFNRPNLYYEIRPKVNVDKEIIKFIKSNPGKS
                     GIIYCLSRKKVEDVAELLVVNGIRALPYHAGLDAATRSSNQDKFLMEEADVIVATIAF
                     GMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGQCVAFYSYKDIQKLEKFMQG
                     KPISEQEIGKQLLMETVSYAESNLCRRKVLLNYFGEDYNHDNCGSCDNCLYPKKQFDG
                     KEDILLVLDLISSMKEQFKAEHIANILAGVSNSIIKSYNHHHHELFGVGSSKGVRYWM
                     AVIRQALIHHLIDKDIERYGLLFCTDNGIKFLENPTTIMLTEDREFMEADEEEEMATA
                     EMRKGGGGGDATLLAMLKDLRLSMAKRLNLPPFVIFNDPSLEDMSIQYPVTLDELKNC
                     QGVGEGKAKKYGKEFIDLISKYVVENEIDRPLDLVVKSVANKSLNKVFIITSIDRKLP
                     FEDIAEAKDLEMNELLEEIESIVNSGTKLNIDYYIRQTVDDDKVDDIYEYFKTEASTD
                     SLKEAVDELGKNYTEEEIRLVKIKFISEVGN"
     gene            complement(1965..3467)
                     /locus_tag="P5293_02110"
     CDS             complement(1965..3467)
                     /locus_tag="P5293_02110"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HRR48733.1"
                     /translation="MDQFLNYNINPIVAYLNKNSSDFTKNDIIKFVTNNGIRLINFRY
                     IAADGRLKTLNLPVTNYKYLDTTLSYGERVDGSNLFPYIDAESSDLYVIPRFSTAYLD
                     PFSTIPTLGLLCSYFNKDGFPLESSPENTLRKAARSFSKKTGFDFEAMGELEFYIVGD
                     DSGKFPIENQKGYHESSPFTKFEQFRCEAVNAIAQSGGEIKYAHSEVGNFTMDGKIYE
                     QNEIEFLVSPVEKAADQLVMAKWIIRNLAFKYGIDVTFAPKITTGKAGSGLHFHTRIV
                     KDGQSLMIKEGGLSEEAKKSIAGFMKCASSLTAFGNTNPMSYFRLVPHQEAPTSICWG
                     DRNRSVLVRVPLGWTGKNDMMNIANPKEDKNQETISDKQTVEMRSPDGSADIYQMLAG
                     LTVAARTGFEMEDALEVVQKTYVDVNIHSKEHAKKLNFLQQLPSSCVQSASELDKMRD
                     IYRKDGVFNDVLLNGVIERLKSFNDENLRSEAEKSPKFLSELVKKYYYCG"
     gene            3588..5801
                     /locus_tag="P5293_02115"
     CDS             3588..5801
                     /locus_tag="P5293_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48734.1"
                     /translation="MRKINKIYITVTFFLLALIVTVVFFYFKNNYNGNNNFVTGFKLS
                     TVSVIPTDAIAVLHFNRISNSIDFLSDTSHMFPPLYPSSSPVGNLINLSVYRGVSQLT
                     VSFHYSAKNEMSPLVILYFDSDSSFTSNSSYILRKMSAIKESSYNGVDILAGGGMKFF
                     KRGRMVVASNSLIILQSSIRHVKSGASIMDNSNFKEIVASTPNGDHMCFFNIQQFGKI
                     FSSFTEYPYWKYADFFSKISSWCSFKYEINGSGVYLSGDMYNTRGRRDFATIFQYAEA
                     GSSQLSDVLPYNTLVALSISVKDFDKLLNSFYEYKRSYKVIDPSLFENDKQWFMSQNP
                     EELVLALLPYKSSYRWVTLIRSKEAVKKRSVTLFEHKGALERLFGKAFSYNNESFVLT
                     FGHWQFIGDEDMLHAFASGNFERYSLKDFVAQGEAYGAMAKLDAPLTVIANVSMMPDS
                     VGNIFREPLNAKISAKLHEKNYQIVIFKLFPYENKTGFSLNLTSQRVAKLREPDTDLV
                     NIPDTVRIPSGPFILSKDENDKLILEQLPDYKLRLKHADGKGIWTIPFEGPLRGFVEE
                     VDYFSHKSYQMLFASGNKLYLLDKRGRFFGRFPKSVDSVIVLGPKVYEKPDGEGFNIM
                     LMHPGNTLRMYDKECHPVEGWKDIVLDETIENFPEMLIVDGKTFWVLRTRTKTMIYRE
                     NGEAVGDLKGDKTLLPNSGIRVLHGTKVAVTSRKGKEVVLDLESGKLSKLKKSDL"
     gene            5808..6032
                     /locus_tag="P5293_02120"
     CDS             5808..6032
                     /locus_tag="P5293_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48735.1"
                     /translation="MGTLLTILLFIILFFWIVGRVLPLMLGWWVKRKLNRFGNQQESS
                     GFSNEKQRKNKIIDKNVGEYVDFVETKEDD"
     gene            6035..7861
                     /locus_tag="P5293_02125"
     CDS             6035..7861
                     /locus_tag="P5293_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR48736.1"
                     /translation="MKTFFRLLSFAKPYGRYWPTYLLISIFSMIFGIFNFALVAPIVR
                     IIFSPNAIVQQLTMPEFSISVDYFTNLFQYYLTKIIGRSSLLNGLLFVSIFMLFMSFC
                     SNLSNYIAQRILVSVKTKLMYNIRKALFLKISRLHIGYFHEKRKGDILSSVSNDVTEV
                     QNTIANTFHILFRDPLMILGFLGALFYMSPQLTAFTLLALPITTFAIARVVRSLRRST
                     VITQSLMGRIISHFEEAISGARIIKAFNAQRYINKRFDITNTSHRKTSKRAFNRQTLA
                     SPLSEFLGVSVATSVLLYGGYLQLDGRLGMDAAAFLVYIGFYWKVLEPTKSISNAFAS
                     LQRGVVSGERIFAILDVDPVIVKAKNPIPIKEFKDSIVFNNVGFKYSTEPVLKNINLE
                     IRKGKMVALVGPSGAGKSTMADLIPRFYDVTSGSITLDGIDIRDYEPKDLIGLMGIVT
                     QEAILFNDTVFNNISFGMENVNEEDVIKAAKIANAHEFITQMENGYYTNIGDRGLKLS
                     GGQRQRLAIARAVLKNPPILILDEATSALDTESERLVQEALVKLMENRTSVVIAHRLS
                     TIQFADEIVVLKSGEIVERGTHQELIKLDGLYSHLCSLQTFA"
     gene            7876..9537
                     /locus_tag="P5293_02130"
     CDS             7876..9537
                     /locus_tag="P5293_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR48737.1"
                     /translation="MEQSLNLIFERSFKENWDNPALSDYKGVTLYYRDVARKIEKLHI
                     VFEICGLKKGDKVSICSKNQANWGVVLLAALTYGAVAVPILHDFKPGNIRHILSHSDS
                     KIFFVGEQVWEQMNGIEISELEAIIQISDFSVLYARDPLIYDTREHLNELFGKKYPKN
                     FRPEHIHYYQDTLDELALINYTSGTTGFSKGVMLPYRSLVSNIMFGWEVHPELNNTSN
                     VVSMLPVAHMYGMMFEFLFEVSVGAHVYFLTKLPSPKIIMEAFAEVKPDIIITVPLII
                     EKIYKIQLQPLINKTAMRVLLRLPVVDQKIGKKIQQELVKAFGGKFQEVIIGGAAFNK
                     EAEAFFKRIGFPFTVGYGMTECGPIISYAPWNKNRLYSCGKAVPRMEIKIDSEDPQNI
                     PGEILCKGMNVMLGYYKNQKATDEVLLKDGWLRTGDIGVMDSDGFLYIRGRSKSVILG
                     PNGQNIYPEEIESIFNNMQYVVESLVIEEKGSLTLLVYPDFEQAEKDGLSQVDLERKM
                     EENRILLNEELTNFSKIQQIKIFPEEFEKTPKKSIKRFLYQRVQE"
     gene            9543..9959
                     /locus_tag="P5293_02135"
     CDS             9543..9959
                     /locus_tag="P5293_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRR48738.1"
                     /translation="MQDKQEQFDRSYLEMAEVWAKNSYCKRRQVGALLVKDRMIISDG
                     YNGTPAGFENICEDDEGHTKPYVLHAEANAITKVARSGNSSLGATLYVTTAPCLECAK
                     LIIQAGIVRLVYKEDYRVSDGIELLRRAGIEIVNIE"
     gene            9961..11562
                     /locus_tag="P5293_02140"
     CDS             9961..11562
                     /locus_tag="P5293_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR48739.1"
                     /translation="MSFFKNFKERMKNNPFIYILIFCLIIFLIFRILDTYSARNRELS
                     VKAGKWDKLLLVLSEVEKNYVDSIDRDTLTEIIIPYLLENLDPHSVYLPPAELKEADE
                     SLEGNFDGIGIQFNIPNDTAIVISVIPGGPSERAGLLSGDRIVKVNDETVAGVKINQD
                     SLVARLRGKSGSIVKIGIKRGNNNNLVYFDIKRDKIPVKSVDVRYMIDDTTGYIKLSK
                     FTKTSYEEFLKAALELKEKGMKELIFDLRGNAGGYFDQAFLIANEFLDKGQMIVYMQG
                     LHRHRQDFKADGKGKLKDIALKVLIDEGSASSSEIFAGAIQDNDRGTVIGRRSFGKGL
                     VQEPIYFSDKSGIRLTVARFYTPTGRSIQKPYSKDYQYDIIERYKHGELTYADSIKKN
                     DSLKYTTPKGKIVYGGGGITPDVFVPIDTVGVNHFLIEVSNKGLPFKFSSMIADAYRP
                     ALQNIRDLQSMETLFSKMNLEQRFLKYAADNKLVPSSKEWEESKYIIIAQIKALIGRY
                     SPLDDEAFYPIMAEIDNVIKKAIEL"
     gene            complement(11537..12283)
                     /locus_tag="P5293_02145"
     CDS             complement(11537..12283)
                     /locus_tag="P5293_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48740.1"
                     /translation="MLKFENKVGLYLTIAVHLLIIIILLLAKISALVSEENSFVIDFS
                     KQEQIEKEAEEIKLKEEISKELDEELSGQTYGSTTRNNIRNVIVDAGAKKGSELRDDR
                     FKNPSQVYQEAMELQKKLEASKKEAEAYQGSDDVPTSTNSSKQSQSESYKGPSVLSYT
                     LEGRKAISLPIPVYKCIAGGDVYISIIVNRKGYVVSASIIDGISANDDCIREYAIRAA
                     KSSRFTASDTAPEKQQGEIVYRFIAQSPFL"
     gene            complement(12276..12896)
                     /gene="udk"
                     /locus_tag="P5293_02150"
     CDS             complement(12276..12896)
                     /gene="udk"
                     /locus_tag="P5293_02150"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRR48741.1"
                     /translation="MLIIGIAGGTGAGKTTVVSQIVSRIRDHVVVIPQDAYYKDNSHL
                     PLEERLELNFDHPDSVDFELLVKQIKELKSGKSIEQPVYSYLTCSRSTTETVRVSPAP
                     IIIVEGILIFTCPELRNCLDIKVFVDADADDRLGRVIARDNLERGRSVEKVLERYEKT
                     VKPMHLQFIEPSKRYADIIIPQGGHNQVAIDILLASIEKFLNKKNA"
     gene            complement(12903..14288)
                     /gene="asnS"
                     /locus_tag="P5293_02155"
     CDS             complement(12903..14288)
                     /gene="asnS"
                     /locus_tag="P5293_02155"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRR48742.1"
                     /translation="MERIKVRELLKLNAGVDVQVKGWVRTKRGNKNISFVALNDGSTI
                     NNIQIVFDMSNFSDEQLKDVTTGACICVEGSLVASVGSRQAVEIQAKKLILYGSADPE
                     KYPLQKKGHSMEFLREIGHLRPRTNTFGAILRIRHAMAFAIHRFFNDKGFYYLHTPLI
                     TGSDAEGAGAMFQVTTLDIDNPPRTPEGKIDFSSDFFGKPTYLTVSGQLEGELGAMAL
                     GEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYEIKENMDLAEEFIKYLVKYALDNC
                     FDDLRFLNDMFDKELIDRLESVINTDFVRLTYTEGVDILMKSGQKFEYPVFWGTDLQS
                     EHERYLVEKHFKKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGGSQRE
                     ESLEKLEKRIAELNMGIESLWWYLDTRRFGTAPHSGFGLGFERLLLFVTGMSNIRDVI
                     PFPRTPKNAEF"
     gene            complement(14347..15846)
                     /locus_tag="P5293_02160"
     CDS             complement(14347..15846)
                     /locus_tag="P5293_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRR48743.1"
                     /translation="MKKSIIIYIAIFLFSLQLYSQDLPKREFRGAWLHTVGNQNFKHM
                     SSDSLKQYLSHTLDVLQQAGINAVIFQVRPQADAFYVSDLEPWSRFIAGEQGKAPEPL
                     WDPLEFMITEAHKRGMELHAWCNPYRVTSNDQEQLHPNHLYFKKPEIFKKYGKQLYFD
                     PGEPEAIAHTKNVIADIVTRYDVDAIHFDDYFYPYPEKFEEFHDDASFVKYAKKQGFE
                     YWQKGDWRRNNVSTLIREINDTIKKIKPWIRFGISPFGVHRNFKDTPDGSGSKTNSLS
                     TYEDLYADIPLWLENGWIDYNVPQLYWKIGHPAADYLTLINWWNDANFKGQLYIGQSI
                     STFNEKDIEDSTKTQFAEKMRLERELPNVDGNVWWPGWSLLTNPFGFTDSLIQKYQKY
                     IALVPAYKSLDSIAPPPVTDLTVKKGIIRWNHNSNNDKMQEARFFAVYRFPLREKIDI
                     DNPKYLLKVVNTNYYKPEVPKGRKVRYQYVITAIDHCSNESEPSKIILL"
     gene            complement(15858..17063)
                     /locus_tag="P5293_02165"
     CDS             complement(15858..17063)
                     /locus_tag="P5293_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRR48744.1"
                     /translation="MKNRFISIIIAIIIFPSTICLGGNDHTKYVFLFIGDGMGISQVA
                     LTETYLASKKGIIGSEPLVFSKFPSFGFCSTHSANYHITCSAAAGTALSTGKKTKNYM
                     LGVDSDTNKLKSITYMLHEKGYSIGIISTVQLEQATPASFYANNANRRNYYEISLQLP
                     KSGFEFVGGGDFSEPRGKNGDKQDIFKILADSNYTIIRGLSEIEKVKNSKKVALFQEE
                     GKESDLPFAIDRKDNDLKLCDVVKAGIDHLSKNKHGFFMMAEGGKIDWAAHSNDAKTD
                     ILEIIDFADAVEVAYQFYLKHPKETLIIVTADHDTGGASLGRNKGGNNLNLDVLNTQD
                     RSFAVDNETIKDNETINTKANIGWTTSGHTGVYVPVYSIGAGDYLFNGRMDNTEIPKK
                     IMKAMNLNF"
     gene            complement(17147..17620)
                     /locus_tag="P5293_02170"
     CDS             complement(17147..17620)
                     /locus_tag="P5293_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRR48745.1"
                     /translation="MIANKKTKTMAPISTSSLPDVVFMLLFFFMITTTIREVTRKVEF
                     KLPEASEAAKLERKDLAAHIYVGTPIRAEQSKYGTDARIQLNDSFKTIDDIRDFIAAE
                     RESLSEADKQFMTVCIKADQNVRMGIITDIKQELRRCSALKIMYSARKPESEPSI"
     gene            complement(17636..18214)
                     /locus_tag="P5293_02175"
     CDS             complement(17636..18214)
                     /locus_tag="P5293_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRR48746.1"
                     /translation="MARPVNQVNASSTADMAFIILVFFLITSNMDSNSGLQRRLPPMP
                     DKNQKVVDQKVNRRNVLVVRINESDRLFAGGQIIDISMLKDKAKEFLLNPNNDPSLPE
                     KEDKFIEGFGMYPVSKGIISLQNTRGTSYDAYIRVQNELVKAVNEVRNQFALEHFGKS
                     FFALDEAKQEIVRKAIPQNISEAEPKDTGKKY"
     gene            complement(18218..18640)
                     /locus_tag="P5293_02180"
     CDS             complement(18218..18640)
                     /locus_tag="P5293_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48747.1"
                     /translation="MQKALKIFSALMFVLIALVAVLFFSNSESEKMIDVILYFGYILL
                     ALSAILALVLPLPLLLQYPKKIKKMLLTILLVVIVCVAGYLLASGAPIEGLMIETPPS
                     AQTLKLTDTALIITYLMLGASILVIIGGGIKSIIQNRK"
BASE COUNT         5653 a         3221 c         3709 g         6144 t
ORIGIN      
        1 gatgctatta ggggttttgt ggaagagagg gaaggtatag ctcatttctt aaattcttct
       61 ctcaccaagt cgcaacttca ggaggtcaag gatgaccttc ttgcgggaaa taccaaattg
      121 ctttatgttg ctccagagtc tcttacaaaa gaggagaaca ttcaacttct caagcagatt
      181 aaaatttcat tttatgctgt tgatgaggct cactgtattt ctgagtgggg ccacgatttt
      241 cgtccagaat atagaaggat tcatcaaatt gccacagata tagggattgc tccaataatt
      301 gctcttactg cgactgcaac tcctaaagtc cagcatgata ttcagaagaa tctggatatg
      361 ctcgaggcta aggtttttaa atcctctttt aacagaccaa acctctatta cgagattagg
      421 cccaaggtta atgttgacaa ggagattatt aagtttataa aatccaatcc gggtaagtcg
      481 ggtattattt actgtcttag cagaaaaaag gtcgaagacg tagcagaact tttggtggtc
      541 aacgggataa gagctttgcc atatcatgca ggtcttgacg ctgctacgag atcttccaat
      601 caggacaaat ttcttatgga ggaggcagat gtgattgttg caacgatagc ttttgggatg
      661 ggtattgaca aacctgatgt gaggtttgtg attcactatg acatacctaa aagtcttgaa
      721 ggatattatc aggagacagg acgtgcagga agagatggtg gtgaagggca gtgtgtggca
      781 ttttattctt ataaggacat tcagaagttg gagaaattca tgcaagggaa accaatatca
      841 gaacaggaga ttggtaagca actgttgatg gaaacagtct cttacgctga atcaaatctt
      901 tgtcgccgta aagttctgct caactatttt ggtgaagatt ataatcatga taattgtgga
      961 tcatgtgata attgtcttta tcccaagaaa caatttgacg gcaaagagga tattttgctt
     1021 gtgctcgatc tgatttcaag tatgaaggag cagtttaagg ctgaacacat tgccaacatt
     1081 cttgctggag taagtaattc aataattaaa tcctacaatc accaccatca cgaactattt
     1141 ggcgttggca gctccaaagg tgtaaggtat tggatggcag tcatcagaca agctcttatt
     1201 catcatttaa ttgacaagga tattgaaagg tatggacttt tgttctgtac tgataatgga
     1261 attaaatttt tagagaatcc aactactata atgcttacag aagacaggga gtttatggaa
     1321 gctgatgaag aggaggagat ggcaactgca gaaatgcgaa aaggcggagg agggggagat
     1381 gctactttgc tggcaatgtt gaaggacttg agactgtcaa tggcaaaaag acttaatctc
     1441 ccaccttttg tcatttttaa tgatccttca cttgaagaca tgtctattca gtacccagtt
     1501 actcttgatg agcttaagaa ctgtcaagga gtaggtgagg gtaaggcaaa aaagtacggg
     1561 aaggaattca ttgatcttat ttcaaagtat gttgtagaaa acgaaataga caggccgttg
     1621 gatttagttg tgaaaagtgt ggccaataaa tctttgaaca aggtttttat aattacaagc
     1681 attgatagaa aactgccatt tgaagacatt gctgaggcca aggatttaga aatgaatgaa
     1741 ctgttggaag aaatagaatc aatagtcaat tcaggcacaa aactcaatat tgattattat
     1801 atacgtcaga ctgtggatga tgacaaagtt gatgatatat acgagtattt caaaacagaa
     1861 gcatccacag actctcttaa agaggctgtg gatgagcttg ggaaaaatta tactgaggaa
     1921 gaaatacgtc ttgtgaaaat aaagtttatc tcagaagtag gcaactaacc acagtaatag
     1981 tattttttaa ccagttccga aagaaattta gggctctttt ctgcttcaga tcttagattt
     2041 tcgtcattga atgatttgag gcgttctata accccgttaa gcagaacatc gttgaaaact
     2101 ccgtcttttc gatagatgtc tctcatttta tcaagttcac tggccgattg gacacatgat
     2161 gaaggaagtt gttgaagaaa attcaatttt tttgcgtgtt ctttgctgtg gatgtttacg
     2221 tcaacatatg tcttttgtac aacttcaaga gcatcttcca tttcgaaccc tgttcgagct
     2281 gctactgtaa gacctgccaa catctggtaa atgtctgctg atccgtctgg tgaccgcatt
     2341 tcaacagttt gtttgtctga aatagtctcc tgatttttat cctctttagg attagcaata
     2401 ttcatcatat cgttttttcc ggtccatccc agaggaactc ttaccagcac agatctgttt
     2461 ctgtctcccc aacatatact tgtgggagcc tcctgatgag gcacaagtct gaaatatgac
     2521 ataggattgg tatttccgaa tgctgtaaga gatgaggcgc atttcataaa tcctgcgata
     2581 cttttttttg cttcttctga aagacctcct tcttttatca tcagcgattg accatctttt
     2641 actattcttg tatgaaaatg aagaccactt ccagccttgc ctgtagttat ttttggtgca
     2701 aaagtcacat ctatcccata tttaaaggca agattgcgaa taatccattt ggccattacg
     2761 agttgatcgg ctgctttttc aaccggtgat acaagaaatt cgatttcgtt ttgttcataa
     2821 attttcccat ccatcgtgaa atttccaact tctgaatgag catattttat ctccccgcct
     2881 gattgtgcta ttgcatttac tgcttcacat ctaaattgct caaattttgt gaatggggaa
     2941 gattcatgat atcctttttg attttcaatt ggaaattttc cactgtcatc tccaacaata
     3001 taaaattcta gctctcccat ggcttcaaaa tcaaaacctg ttttcttgct gaaggatctt
     3061 gcagctttgc ggagagtgtt ctcaggagaa ctttccaaag gaaaaccgtc tttgttgaag
     3121 taggaacaaa gaagtccaag cgttggaatt gtcgaaaaag gatctaaata tgcagttgaa
     3181 aatctaggta ttacataaag atcgctcgat tcagcatcga tatagggaaa gagattggaa
     3241 ccgtcaactc tttctccata agaaagagta gtatcaaggt atttataatt tgttactggc
     3301 aggtttaatg tttttaatct cccatcagcg gcgatatatc tgaaatttat cagtcgtatt
     3361 ccgttgtttg ttacaaattt aatgatatca tttttagtaa aatctgatga atttttattt
     3421 aaatatgcaa caataggatt aatgttgtag ttaagaaact gatccataaa tgtgataaaa
     3481 ttttattatt ttttttattt tgaaacgaaa ttaatgcaaa aaagtaacga tttggataat
     3541 ttattgcttt ttgtcacctt ttttattttt aatagctttg cagcctaatg agaaagataa
     3601 ataaaatata tattacagtt accttttttt tgcttgccct tattgttact gtcgtttttt
     3661 tttattttaa aaacaattat aatggtaata ataattttgt caccgggttt aagttaagta
     3721 cagtctctgt cattcctacc gatgctattg ctgttttgca ttttaacaga attagcaatt
     3781 ccatcgattt tctgtcagat acatctcata tgtttcctcc tttatatcct tcttcgtcgc
     3841 ctgtaggaaa tttaattaat ctttcagtct acagaggagt atcacaactg actgtttcat
     3901 ttcactattc tgccaaaaat gagatgtctc cattagttat actgtatttt gattcggatt
     3961 cttctttcac ttcaaattcc agttatattc tcaggaagat gtctgccatt aaggagagtt
     4021 catataatgg tgtagatata cttgcaggag gggggatgaa attttttaaa cgaggaagga
     4081 tggtagttgc cagtaattct ctgattattc ttcagtcttc aattagacat gttaaaagtg
     4141 gtgcctccat tatggataat tctaatttca aagaaatagt tgcatctact ccaaatggag
     4201 atcacatgtg cttttttaat attcagcaat ttgggaaaat tttttcgagc ttcactgaat
     4261 atccttactg gaaatatgct gatttttttt caaaaatctc ttcttggtgt tcttttaaat
     4321 atgaaattaa tggtagtggt gtatacttat cgggagatat gtataataca agaggaagac
     4381 gagattttgc aaccatattc caatatgccg aagcaggcag ttcccagttg agtgatgttt
     4441 tgccatataa cacattggtt gccttatcaa tatcggttaa ggactttgat aaacttctta
     4501 attcgtttta tgaatataag cgttcataca aggttattga tccgtcgtta tttgaaaacg
     4561 ataagcagtg gtttatgtca caaaatccag aagaacttgt gcttgctctt ttaccatata
     4621 agagcagtta cagatgggtc actcttatta ggagtaaaga agcagtgaag aagagatctg
     4681 ttactttatt tgaacataag ggggcacttg agagattgtt tggcaaggca ttttcttaca
     4741 ataatgaaag ttttgtttta acgtttggtc attggcagtt tattggagac gaggatatgt
     4801 tgcatgcatt tgcttccggt aattttgaaa gatattcctt aaaagatttt gtcgctcagg
     4861 gggaggctta tggtgccatg gcaaaattgg atgcaccatt gacagtcatt gcaaatgtgt
     4921 ctatgatgcc tgattcggtt ggaaacattt ttcgtgaacc tctcaatgcc aaaatttccg
     4981 caaaattgca tgaaaaaaat tatcagattg taatttttaa actttttcct tatgaaaata
     5041 aaacagggtt ttccttgaat cttacttctc aaagagtagc caagctaaga gaaccggata
     5101 cagaccttgt gaatatacca gatacagtca gaataccttc gggacctttt atattgtcaa
     5161 aagatgaaaa tgacaaatta attcttgaac agttaccaga ttataaattg agattaaaac
     5221 atgcggatgg taaagggatt tggacaattc cgtttgaagg acctctgaga ggatttgttg
     5281 aagaggtaga ttatttttca cataagagtt atcaaatgct gtttgcaagt ggaaacaaac
     5341 tatatttgct ggataaaaga ggtcgatttt tcgggaggtt tccaaagagt gttgattcag
     5401 tcattgtcct tggtccgaaa gtttacgaaa aacctgatgg agaaggattt aacatcatgc
     5461 tcatgcatcc gggcaacact ttaaggatgt atgacaaaga atgtcatcct gttgaaggct
     5521 ggaaagatat tgttttagat gaaactattg aaaattttcc cgaaatgctg attgtggatg
     5581 gtaagacatt ctgggttttg agaaccagga ctaaaactat gatttaccgg gaaaatggag
     5641 aagctgttgg agatcttaaa ggcgataaga ctctccttcc taattccggg atacgtgtgc
     5701 tgcatggcac aaaagtggca gttacctctc gcaagggaaa ggaggttgta ttagaccttg
     5761 aaagcggaaa attaagtaaa ttgaaaaaga gcgacttata attataaatg ggaacattat
     5821 taactattct tctttttata atacttttct tttggattgt ggggagagtg ttgccactca
     5881 tgctgggttg gtgggttaaa aggaagttga acagatttgg caatcaacaa gaaagttcgg
     5941 gattttcaaa cgagaagcag cgtaaaaata aaataataga caaaaacgtc ggtgagtatg
     6001 tggattttgt agagacaaaa gaagatgatt gagtatgaaa acttttttta gattgctgtc
     6061 cttcgcaaag ccatatggga gatattggcc gacatatttg cttatctcta ttttttccat
     6121 gatttttggg atttttaatt ttgccctcgt tgcaccaatt gtaagaataa tattcagtcc
     6181 taatgcaata gttcagcagt tgaccatgcc tgaattctcc ataagtgtcg actactttac
     6241 taatcttttt cagtattatc taactaagat aattggaaga tcttctttat tgaacggctt
     6301 gctgtttgtt tctattttca tgctgtttat gtctttttgt tccaacctgt caaactatat
     6361 tgctcaaagg atacttgtaa gtgtgaagac caaactgatg tataacattc gtaaagccct
     6421 gtttcttaag atttcaagac ttcacatagg ctattttcat gagaagagaa agggggatat
     6481 cttatcaagc gtctcgaatg atgtcactga agttcagaat actattgcaa acactttcca
     6541 tatacttttc agggatccgt tgatgatttt aggttttctg ggggcacttt tttacatgtc
     6601 gccacagtta actgctttta ctcttctagc attgccgatt acaacatttg cgattgccag
     6661 agttgtgagg tctcttagaa gaagtactgt gattactcag tcactgatgg gaagaattat
     6721 aagccatttt gaggaagcca tttcaggtgc aagaattatt aaagctttta atgcgcagag
     6781 atatataaac aaacgctttg acatcacaaa tacaagtcac aggaagacaa gtaaaagggc
     6841 ctttaacagg cagacacttg cttctccgct ttctgaattt ttgggagtat ctgtcgctac
     6901 ttctgttctc ttatatggcg gttatcttca attggatgga agacttggta tggatgctgc
     6961 agctttttta gtttatattg gtttttattg gaaagttctg gaacctacaa aatcaatttc
     7021 taatgctttc gctagtcttc aaagaggtgt agtctcagga gagagaattt ttgcaattct
     7081 tgatgttgat cctgtaatag taaaggctaa aaatcctatt cctataaaag agtttaagga
     7141 tagtatcgtt tttaacaatg ttggtttcaa atatagtact gaacctgttc ttaaaaatat
     7201 taatcttgaa ataagaaaag ggaaaatggt agctcttgta ggaccttccg gtgcaggtaa
     7261 atcaacaatg gcggatttga ttcctcgttt ttatgatgtc acttctggct caataactct
     7321 tgatggcata gatataaggg attatgaacc gaaggatctt ataggcctaa tggggattgt
     7381 gactcaggag gccattctgt ttaatgacac tgtttttaat aacatatcct ttggaatgga
     7441 aaatgtaaat gaggaggatg taattaaagc tgcaaaaatt gcaaatgcac acgaatttat
     7501 tacacaaatg gagaatggat attatacgaa cattggagac agaggactta aactttctgg
     7561 agggcaaagg caaaggcttg ctattgcaag agcagtgctg aaaaaccctc ctatccttat
     7621 acttgatgaa gctacctcag cattggatac tgaaagcgaa aggcttgtac aggaggcttt
     7681 agtcaagttg atggagaaca gaacatctgt tgtgattgca cacagacttt ccacaattca
     7741 gtttgctgat gaaatagttg ttctaaaatc aggtgagatt gttgagagag gtactcatca
     7801 ggaacttatc aaactcgatg gcctgtattc tcatctgtgc agccttcaga catttgccta
     7861 atatttttga agtatatgga acaatcatta aatctcattt ttgagagaag ttttaaagaa
     7921 aattgggaca atccagctct atctgattac aagggtgtta cattatatta cagagacgtt
     7981 gcccgtaaaa tcgagaagtt gcacatagta ttcgaaattt gtgggttaaa gaaaggcgac
     8041 aaggtttcta tttgttcaaa gaatcaggct aactggggag ttgttcttct tgcagcgctt
     8101 acttatggtg cagtagcggt gcctattttg catgatttca agccggggaa tattaggcat
     8161 atattaagtc actcggattc caagattttt tttgttggag agcaagtttg ggagcagatg
     8221 aatggtatag aaatatcaga actggaagca attattcaaa tatccgattt ttctgtgctg
     8281 tatgccaggg atccacttat atatgatacc agggaacatt taaacgaatt gtttgggaaa
     8341 aaatatccga agaatttcag gccagagcat attcattatt accaggatac tcttgatgaa
     8401 cttgctttga taaactacac ttcaggcaca acaggctttt caaaaggggt aatgcttcca
     8461 tatagatctc tagtttcaaa cattatgttt ggctgggaag tgcatcctga attaaacaat
     8521 acatcaaatg tggtttccat gcttcctgtt gctcatatgt atggaatgat gtttgaattt
     8581 ctttttgagg tgtctgttgg agctcacgtc tattttctca caaagttacc aagccctaag
     8641 ataattatgg aagcattcgc tgaggtgaag cctgacataa taataactgt gcctttgatt
     8701 attgagaaga tatacaaaat acaacttcaa cctcttatca ataaaactgc gatgagggta
     8761 cttctccggc ttccggtagt ggatcaaaaa attggcaaga agatacagca ggagcttgta
     8821 aaagcctttg gcggaaagtt tcaggaagtt attattggag gtgctgcttt caataaagag
     8881 gctgaagctt ttttcaaaag aattgggttt ccgtttaccg ttggttacgg gatgactgag
     8941 tgtggcccaa ttatctctta tgcaccatgg aataaaaata gattgtattc atgtggtaaa
     9001 gctgttccga ggatggaaat caagattgat tcggaggatc cgcagaatat acctggtgag
     9061 attttatgta agggtatgaa tgtcatgctt ggctattata agaaccagaa agctactgat
     9121 gaggtgcttt taaaggatgg gtggcttaga accggagata tcggggtgat ggacagtgat
     9181 ggatttcttt acataagggg aagatcaaaa agtgtgattt taggacctaa tggtcaaaat
     9241 atttatcctg aagagattga aagtattttt aacaatatgc agtatgttgt agaatctttg
     9301 gtgattgaag aaaagggttc tttgacattg cttgtttatc ctgattttga gcaggccgaa
     9361 aaggatgggc tttcacaggt agaccttgaa agaaaaatgg aagaaaatcg cattttactc
     9421 aatgaagagc tcactaattt cagtaaaata caacaaatta aaatctttcc ggaggaattt
     9481 gaaaaaactc cgaaaaaaag tataaaaaga tttttatatc aaagagttca ggagtaagtt
     9541 ttatgcaaga caaacaggaa cagtttgaca gaagctatct ggagatggct gaagtatggg
     9601 cgaagaattc atattgtaag agaagacagg taggagcatt actggttaag gacagaatga
     9661 tcatttctga tggatacaat ggtactcctg ccggttttga gaatatttgt gaagacgatg
     9721 aaggacacac aaagccttat gtgcttcatg cagaagcaaa cgcaattact aaagttgccc
     9781 gatctggtaa tagtagcctt ggagccactt tatatgtaac tactgctcct tgtttggagt
     9841 gcgccaaact gataattcaa gcaggtattg taaggctggt ttacaaggaa gattacagag
     9901 tctctgatgg aattgaactt cttagaagag caggaataga aattgttaat attgaataat
     9961 atgtcatttt tcaagaattt caaagagagg atgaagaata atccttttat atatatctta
    10021 atattttgtt tgataatttt tctgatattc agaatattgg atacatattc ggccagaaac
    10081 agagagttgt ccgtaaaagc aggaaaatgg gataaacttt tgttggtgct ctctgaagtt
    10141 gagaaaaatt atgtcgactc cattgacaga gacactttga ctgaaattat tattccatat
    10201 cttctcgaaa atctcgatcc tcattcggta taccttcctc ctgcagaact taaagaggca
    10261 gatgaatctc tggagggtaa ttttgacggt ataggtattc agtttaatat tcctaatgat
    10321 acggcaatag ttatcagtgt aatacctggt ggtccttccg agagagctgg tttgctttca
    10381 ggtgacagaa ttgtaaaagt aaatgatgaa acagttgcag gagttaaaat aaatcaggat
    10441 tctcttgtgg caagacttag aggtaaatcg ggttctattg ttaagattgg catcaaacgg
    10501 ggtaataaca acaatttggt atattttgac atcaaaagag acaagatacc tgttaaaagc
    10561 gtggacgtaa gatatatgat tgatgacaca accgggtata tcaaattgtc taagtttaca
    10621 aaaaccagct atgaagagtt tttgaaagca gctcttgaac ttaaagagaa aggtatgaag
    10681 gaattgattt ttgatttaag aggtaatgca ggtggttatt ttgatcaggc ttttcttatt
    10741 gccaacgaat tccttgataa gggacaaatg attgtatata tgcaaggtct tcacagacat
    10801 agacaagatt ttaaagctga tggaaagggt aaactgaaag atattgcttt aaaggttctt
    10861 attgatgaag gatcagcctc ttcaagtgaa atttttgccg gagccattca ggataatgat
    10921 aggggaacag ttattggaag acgttcattt ggaaagggac ttgttcagga gccgatttat
    10981 ttttctgaca agtctggaat aagacttact gtagcgaggt tctatactcc gacaggcagg
    11041 tctattcaaa aaccttattc aaaggattat cagtatgata taattgaacg atacaagcat
    11101 ggagaactga catatgctga tagcatcaaa aagaatgact ctcttaagta cacaactcct
    11161 aaaggaaaga ttgtatatgg cggaggaggt attactccgg atgtatttgt gcctatagac
    11221 actgtgggag tgaatcattt tttgatagaa gtctcaaaca agggtttgcc ttttaagttc
    11281 agttctatga tcgcagatgc ttatagacct gctttacaaa acataaggga tcttcaatcc
    11341 atggaaactc ttttttcaaa gatgaatctg gagcagaggt ttctaaaata tgctgcagac
    11401 aataagctgg taccttcttc taaggagtgg gaggagagta aatatataat aatagctcag
    11461 ataaaggcgc taataggcag atattctcca ctagatgacg aagcttttta tccaataatg
    11521 gctgaaattg ataatgttat aaaaaaggcg attgagctat gaatctgtaa acgatctctc
    11581 cctgctgctt ttctggagca gtgtcagatg ccgtaaatcg agatgattta gctgctcgta
    11641 tggcgtattc tcttatgcag tcgtcatttg cagatatccc gtctataatt gatgcagaga
    11701 ctacgtatcc ctttctattg acaataattg aaatatagac atctccacct gctatgcatt
    11761 tataaacagg aattggcagg gatattgctt ttcttccttc gagagtatat gatagaactg
    11821 aagggccttt gtatgactca ctttgagact gtttggagct attggtagaa gttggaacgt
    11881 catcactgcc ctgataagct tctgcctctt tttttgaagc ttcgagtttc ttctggagct
    11941 ccattgcttc ttgatagact tgtgagggat ttttgaaacg gtcgtctctt aattcacttc
    12001 cctttttagc tcctgcatcg acaatcacat ttcgaatatt atttctggtg gttgaaccgt
    12061 aagtctgccc tgaaagttct tcatccaatt ctttgctgat ctcttcttta agtttaatct
    12121 cttcagcctc tttttctatt tgttcctgtt ttgaaaaatc aattacgaag gagttttctt
    12181 ctgatacgag agcgctaatt ttggctagca ggagaattat aattatcaga agatgaactg
    12241 caattgtaag atagagacca actttgtttt caaatttaag catttttctt gttgagaaac
    12301 ttttctattg atgcaagcaa gatatcaata gcaacctgat tgtgacctcc ttgcggaata
    12361 atgatatcag cgtatctttt acttggttct atgaattgaa gatgcatcgg ttttacagtc
    12421 ttttcgtatc tttcaaggac tttttcaaca gaccttcctc tttcaagatt gtctcgagca
    12481 attaccctgc caagcctgtc atcagcatct gcatcgacaa agacctttat atccagacag
    12541 tttctcagtt caggacaggt gaaaatgagt attccttcca caataattat gggagcaggt
    12601 gatactctta ccgtctcagt agtcgatctt gagcatgtaa gataagaata aactggctgt
    12661 tcaatggatt ttcctgactt aagttccttg atttgtttga caagaagctc gaagtcgact
    12721 gaatccggat ggtcaaaatt aagttcaagt ctctcttcaa gtgggaggtg actgttatcc
    12781 ttataatagg catcttgagg aataactact acatgatctc taatcctgct tacaatctga
    12841 gatacaacag tagtttttcc tgctcccgta ccgccggcaa ttccaattat tagcatatgt
    12901 tattaaaatt cagcattttt aggcgttcta gggaatggaa tcacatctct aatatttgac
    12961 atgcctgtaa caaaaagcaa aagcctttca aatccaagtc cgaaaccgga atgaggagct
    13021 gtcccgaatc ttctggtatc gagataccac caaagtgatt caatacccat gttaagttct
    13081 gcaattcttt tttcaagttt ttccagagat tcttcacgtt gagagccacc tattatctct
    13141 ccaatctttg gaaacagaac atccattgcc cttacagttt taccgtcatc attctgtttc
    13201 atatagaaag ctttaatctc ttttgggtag tctgtgagga ttactggttt tttaaaatgt
    13261 ttctcaacaa ggtatctttc atgttcactc tgcaaatcag taccccagaa aacgggatat
    13321 tcaaattttt gtcccgattt catgagaata tcaactcctt cggtataggt tagtcttaca
    13381 aaatcagtgt tgataacgct ctcaagtcta tcaatcagtt ctttatcaaa catatcattt
    13441 aagaacctta agtcgtcaaa gcaattgtcc agagcatatt ttacgagata tttgatgaac
    13501 tcttctgcaa gatccatatt ctccttgatc tcataaaatg ccatttccgg ttcaatcatc
    13561 caaaattcag caagatgtct tggtgtattg gaattttctg ctctaaatgt aggtccgaag
    13621 gtataaattt ctccaagagc catagctcct aattctcctt caagttgtcc gctgactgta
    13681 agatatgtgg gcttgccaaa aaaatcagag ctaaaatcta tttttccttc aggtgttctc
    13741 ggaggattgt caatatcgag agtcgtcacc tgaaacattg ctcctgcacc ttctgcatca
    13801 gaaccggtta tcagaggagt atggaggtaa taaaaacctt tgtcgttaaa gaacctatga
    13861 atagcaaaag ccattgcatg gcggatacgg agtatagctc caaaggtgtt ggtacgtggc
    13921 ctcagatgtc caatttcacg taaaaattcc atactgtggc cttttttctg aagaggatat
    13981 ttctccggat cggctgaacc atacaatata agtttttttg cctgaatctc aacagcctgt
    14041 ctactgccaa cagatgcgac cagagaaccc tcgacgcaaa tacacgctcc agttgtcaca
    14101 tccttgagct gttcatctga gaaattgctc atatcaaaca caatctgtat attattgatt
    14161 gtggagccat catttagagc gacgaaagat atgtttttat tgcctctttt tgttcttacc
    14221 catcctttaa cttgtacatc gacacctgca ttcagtttca acagttcgcg aaccttaatt
    14281 ctttccatat aattttgata acaactctat ttatatatca agcaaagtta ctcaattggt
    14341 aggaaatcac aataatatta ttttggatgg ttcactctcg ttggagcaat ggtcaatggc
    14401 agtgataaca tattgatatc taacttttct gccctttgga acttcgggtt tgtagtaatt
    14461 tgtattaacg acttttagca gatatttcgg attatctata tcaatttttt ctctgagagg
    14521 gaagcgatat actgcaaaaa atctggcctc ttgcattttg tcattattgc tattgtgatt
    14581 ccaccttata ataccctttt tcactgtcaa atctgtcact ggtggaggtg cgattgagtc
    14641 aagagattta tatgcaggaa caagggcaat gtatttttga tatttttgaa taagagagtc
    14701 agtaaatcca aatgggtttg taagtaatga ccagccaggc caccatacgt ttccatcaac
    14761 atttggcagc tctctttcaa gtctcatctt ttcagcaaac tgagtcttgg tagaatcttc
    14821 gatgtctttt tcattgaatg tactgatact ttgtcctatg tagagctgtc ccttaaaatt
    14881 ggcgtcattc caccaattaa tcaatgtcaa ataatctgca gctgggtgcc ctattttcca
    14941 gtaaagctgt ggaacattgt agtctatcca tccattttcc agccaaaggg gaatgtcggc
    15001 atataggtcc tcataagtag aaaggctatt tgttttgctt ccgcttccat ctggagtatc
    15061 tttaaagttt ctgtggactc cgaagggact gatcccaaat cttatccatg gcttgatttt
    15121 cttgattgta tcattaattt ctcttatgag agttgatacg ttatttcttc tccagtcacc
    15181 tttttgccag tattcaaatc cctgtttttt agcatatttc acaaatgatg catcatcatg
    15241 aaattcttca aatttttccg gatagggata aaagtaatcg tcaaagtgaa ttgcatcaac
    15301 atcatatctt gtcacaatat ctgcaataac attttttgta tgagctattg cttctggttc
    15361 acccgggtca aagtaaagct gtttgccata ttttttgaat atttcaggtt ttttgaaata
    15421 gagatgattt ggatggagtt gctcctgatc atttgaagtt acacgatatg ggttacacca
    15481 ggcatgtaat tccatacctc ttttatgagc ttctgtaatc ataaattcaa gaggatccca
    15541 tagtggttcc ggcgcctttc cctgttcacc ggcaataaat ctgctccatg gctccaaatc
    15601 tgatacataa aatgcatcag cttgtggtct tacctgaaaa atcacagcat taattccagc
    15661 ttgttgaagg acgtcaagag tatggctgag gtactgtttc aatgaatcac tgctcatatg
    15721 cttgaaattt tgatttccca ccgtatgaag ccatgcgcca cgaaattctc tcttaggcaa
    15781 atcttgagaa tagagttgga gagaaaaaag aaaaattgct atataaataa taatactttt
    15841 tttcatagtt tatattttta gaaattgaga ttcatagctt tcatgatttt tttaggaatt
    15901 tcggtattat ccattctccc gttaaagaga taatctccgg cacctatcga ataaacagga
    15961 acatatactc ctgtgtgacc tgatgttgtc caaccaatgt ttgccttagt atttattgtt
    16021 tcgttatctt tgattgtctc gttatcaacg gcaaaagatc tatcttgcgt attcaaaaca
    16081 tccaaattaa gattgttgcc gcccttattt cttccaagtg aagctcctcc tgtatcgtga
    16141 tccgcagtaa ctataataag agtctcttta ggatgcttca gataaaattg gtatgcaact
    16201 tctacggcgt cagcaaaatc gattatttcg agaatatccg ttttggcatc gttactgtgt
    16261 gcagcccaat ctattttacc tccttctgcc atcataaaaa aaccatgttt gtttttactc
    16321 aaatgatcaa tgccggcttt aaccacatcg cataatttga ggtcgttatc ttttctgtca
    16381 atggcaaacg gaagatcaga ttctttgcct tcctcctgaa ataaagcaac tttttttgaa
    16441 tttttaactt tttcaatttc ggacaatcct cttattatgg tatagttact atctgctaga
    16501 atcttgaaaa tatcttgttt atctccattt ttgccccttg gttcagaaaa atcaccccct
    16561 ccaacaaatt caaaaccact ctttggcaat tggagagata tttcgtaata gtttcttctg
    16621 ttggcattgt tcgcataaaa tgatgcaggt gttgcttgtt caagttgaac tgttgatatt
    16681 attccaatag aatagccctt ttcatgaagc atgtacgtta tactctttaa cttgttggtg
    16741 tcagaatcaa cacctaacat atagttcttc gttttctttc cagtagaaag agctgttcct
    16801 gctgctgctg aacatgtaat atgataattt gcagaatgtg ttgagcaaaa cccaaatgat
    16861 ggaaattttg aaaagacaag tggttcagat ccgataatgc ctttttttga ggcaagatat
    16921 gtttctgtta gtgcaacttg agaaattccc atgccatcgc caataaaaag aaagacatac
    16981 tttgtatggt cattacctcc taaacatatt gttgatggaa aaattattat tgcaatgata
    17041 attgaaatga atctgttttt cattaaaaaa gggtggttta aatataacaa taaagggtgc
    17101 cctaaattta aataggtgca cccttatagt acaattgtat aggcaattat atgctaggtt
    17161 cagattccgg ttttcttgca gagtacataa ttttaagagc tgaacacctt ctaagttcct
    17221 gcttaatatc agtgataata cccattctga cattttgatc agcctttatg catactgtca
    17281 tgaactgctt gtcagcttct gatagggatt ctctttctgc agcaataaaa tcacgtatat
    17341 catcgatagt cttgaaagaa tcgtttagct gtattcgagc atcagtaccg tatttacttt
    17401 gttcagcacg gataggtgtt ccaacataaa tatgcgcagc caaatctttt ctttcaagtt
    17461 ttgcagcctc agaagcttca ggtaatttga attcaacctt ccttgtaacc tcccggatag
    17521 ttgttgtaat cataaagaag aacagcaaca tgaacacaac atcaggcaat gacgacgtgc
    17581 taatcggcgc cattgtttta gtttttttat ttgcaatcat attatattct cctttctaat
    17641 attttttacc agtatccttt ggttctgctt cagaaatgtt ctgagggata gcctttctta
    17701 ctatttcctg tttggcttcg tctagagcaa agaatgactt gccaaaatgc tcaagagcga
    17761 attgatttcg gacctcatta actgctttga ccaattcgtt ttgaaccctt atataggcat
    17821 catagcttgt cccacgagta ttctgaagtg agataatacc tttggaaaca ggatacatcc
    17881 caaacccttc aatgaattta tcctcttttt cagggagaga tggatcattg ttgggattta
    17941 aaaggaactc ctttgccttg tctttcaaca tagatatgtc aatgatctgg cctcctgcaa
    18001 aaagtctgtc agattcatta atccttacaa ccaaaacatt tctacggttg accttctgat
    18061 ctacaacctt ctgattctta tcaggcattg gtggtaaacg acgctgaagg ccagaattgg
    18121 agtccatgtt cgatgttata aggaaaaaca ccagaattat gaaagccata tcggcagtag
    18181 agctcgcgtt aacttgattt actggtcttg ccatgtttta ttttctgttt tggataatgg
    18241 atttgatacc tcctcctata ataactaaaa tcgaagctcc aagcataaga taagtaatga
    18301 taagcgcagt atcggtaagt ttaagagttt gtgcacttgg aggtgtttca atcataagtc
    18361 cttcaatagg tgctccggaa gctaaaagat aaccggctac acacacaata actactagaa
    18421 gaatggtcaa cagcatcttc ttaatttttt ttggatattg taaaagaaga ggcagcggaa
    18481 gaaccaatgc tagaattgct gataaggcca acagtatata tccaaaataa agaataacat
    18541 ctatcatctt ttctgattcg gaattagaaa agaataatac tgccaccaaa gcaatcagca
    18601 caaacattaa tgctgaaaat atttttaaag ctttttgcat aatctgctat tttttacttt
    18661 ttacaagcat gtctatcagt gaaatagatg agtcttccat cgaaagaact atagagtcaa
    18721 tctttga
//
LOCUS       DAOTQL010000017        18028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3047_length_18028_cov_5.367607, whole genome shotgun sequence.
ACCESSION   DAOTQL010000017 DAOTQL010000000
VERSION     DAOTQL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3047_length_18028_cov_5.367607"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="P5293_02185"
     CDS             complement(<1..1577)
                     /locus_tag="P5293_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HRR48748.1"
                     /translation="MEVTRTFDLLERLVTLYPKDDIFSRKHNGIWINCSSQEYKEKSH
                     ILAYAFLSLGLKPFDKVITICNNRQEWNFIDMGLSLAHLVHVPVYPTLGNDEYQHIFN
                     HSDAKIIIVGNESIYKRIKPAVSSMNTPPDVYSIDEVPGIKSLSELFTLGKNNSQRYF
                     SQIEENKRTIDENDLVTIIYTSGTTGTPKGVMLSHKNLVFTFIGSAVQQIRDHNHKML
                     SFLPLCHVYERSMNYDYQYLGISTYYAESLGTIAADLADCHADGFCAVPRVLEVMYSK
                     FESAGKDLKGIAKLIYKWAFNIANKYDYTKNGVLYKTMYGLADKLVYSKWREKLGGKE
                     MLVVSGGSAIQEKIVRLFTAAKMYIFEGYGMTETSPIIAVNNPKEMIIKIGTVGKPTV
                     GTDLKIAEDGEILTRGPHVMLGYYKDPEYTKSVIDEEGFFHTGDIGTIIDGIFLKITD
                     RKKEIFKLSSGKYIAPQVIEMMLNESSYIENCMVVGENQKFASAVIVPNFENLRTWAT
                     KNKISFTTNEELINNSE"
     gene            complement(1602..2639)
                     /locus_tag="P5293_02190"
     CDS             complement(1602..2639)
                     /locus_tag="P5293_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRR48749.1"
                     /translation="MKHSFGSDNHSGVAPEIMEAIIEANEGFSVAYGDDETTAKAIRI
                     FKEKLGENAEPFFVFNGTGANVTALKTLTKSHNSILCPDTAHINVDECGAPEKATGCK
                     LIPLHSTDGKVNPEEVRKELVNFGVQHHSQVKVLSISQPTELGTLYTPGEIRSLADLM
                     HSYDCFLHMDGSRISNAAAALGLSIKAITADLGVDALSFGGTKNGLLIGEAVIFFKKP
                     FAEDFPYVRKQNTQLYSKNRFIAAQFIAYLKDDLNLKLARHSNEMAKYLEYKLQGINE
                     VKISRPVMTNAVFAIIPEFLSDDLMSTHNFSVWNEATNEVRWMCSFNTAKEDVDNFVS
                     DIKQSILKSKA"
     gene            complement(2730..3104)
                     /gene="gcvH"
                     /locus_tag="P5293_02195"
     CDS             complement(2730..3104)
                     /gene="gcvH"
                     /locus_tag="P5293_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRR48750.1"
                     /translation="MNIPANLKYSKDHEWVKVDGNIATVGITDFAQNQLGDIVFVDIQ
                     TEGETLDASEIFGAIEAVKTVADALMPVSGKIIEINPELEDAPELVNTDPYGKGWMIK
                     IEISDPSELNSLLDAESYKAIL"
     gene            complement(3149..3739)
                     /gene="ruvA"
                     /locus_tag="P5293_02200"
     CDS             complement(3149..3739)
                     /gene="ruvA"
                     /locus_tag="P5293_02200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRR48751.1"
                     /translation="MFDYIKGKVVELNPTEVVIETNNFGYRILISLQTFSQLTPDTEC
                     KLYLYHHIREDTELLYGFFDKEERYIFSLLINVTGVGPNTAKLMLSSLSTEELRNAII
                     SDDINKIKSVKGIGLKTAQKIIIDLKDKIIKGTPSGKTGVISVSPVSQIREEASSALV
                     LLGFPKQNVEKVIDSILKENPNQKLETLIKEALKRV"
     gene            3837..5057
                     /locus_tag="P5293_02205"
     CDS             3837..5057
                     /locus_tag="P5293_02205"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HRR48752.1"
                     /translation="MKDVLDIRRNFPALEQSVNGKPLVYFDNGATSQKPRSVIELVNR
                     MNSSVNGNIHRAVHELSARATEIYENSREEIRKFINAVSREEVIFTSGTTASINLVAN
                     SFSRVFIDKGDVVLITEGEHHSNIVPWQMACERYGAELRVLPVDDNGCWRMEQLDSLL
                     MDGKVKIASVAHVSNVLGVVNPVNTLIEICHKAGVPVLLDGAQGIIHLNVDVQKLDCD
                     FYVFSGHKLFGPTGIGILYGKKDFLNAIPPWMGGGDMVGRVSFEKTTYAELPLKFEAG
                     TPNFIGAAGLGEAIRFINNIDSEFVKEHERQLVERMMNGLSSVGSLIIYGTSDSKVPL
                     FSFNVPGIHPTDMAMLMDKMGIALRSGMMCSEPLMDHFGVSGMLRASLTIYNNLEEVD
                     YFIESLQKAIRMLS"
     gene            5084..5503
                     /locus_tag="P5293_02210"
     CDS             5084..5503
                     /locus_tag="P5293_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRR48753.1"
                     /translation="MTIKEVEQQIIDEFSVFTDWLDKYEYLIELGKSLPLIDEKKKID
                     ENLIRGCQSKVWLAADYVDGKIYFTADSDAIITKGIISLLIRVFSGRTPQEILDADLN
                     FIKEIGLQDNLSPTRSNGLVSMIKQIKLYALAFSANK"
     gene            5512..5823
                     /locus_tag="P5293_02215"
     CDS             5512..5823
                     /locus_tag="P5293_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HRR48754.1"
                     /translation="MADTSVEKNIVLALKQVYDPEIPVNVYDLGLIYDLKVDDSGKVF
                     IKMTLTAPNCPIMDDLLQDIHDAVMDVPSVTDVAIELVFDPPWDKSRMSQEAQLELGF
                     L"
     gene            5827..6327
                     /gene="folK"
                     /locus_tag="P5293_02220"
     CDS             5827..6327
                     /gene="folK"
                     /locus_tag="P5293_02220"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRR48755.1"
                     /translation="MEKVILSLGSNKGDRLKYLRSAIDRIGDCIYKTPKISQIYESEP
                     WGFNSTVWFLNIAMLLETDLSPIELLDELNIIEKELGRVRKSNASDYESRVIDIDIIF
                     FGDRVLDQARLIIPHPFAHLRRFVLLPVLDISPHYIHPVLNKTIRELVESCPDKSELR
                     LFCHEI"
     gene            6402..7031
                     /locus_tag="P5293_02225"
     CDS             6402..7031
                     /locus_tag="P5293_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRR48756.1"
                     /translation="MNIIEGLQWRYATKRFDTDKKLNEEQIEYLKQAVSLIPSSYGLQ
                     PYKALIVEDPEIRKKLSEAAFGQPAITEASHLFIFANFTEYGPDKVESFLRLSAEINN
                     YPEGSSDTYRDMLNGMIGAMTPEQLSNWTAKQAYIALGALICAASEQHIDICPMEGFD
                     KSQFDDILELRQKGLTAACIGAVGYRSPQDRHQFLKKVRKPLDQLFEVI"
     gene            complement(7014..8981)
                     /locus_tag="P5293_02230"
     CDS             complement(7014..8981)
                     /locus_tag="P5293_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRR48757.1"
                     /translation="MKRLILLLFSILSINLIGAQTSTTTWTFTQKDNEDGTVDLIFKA
                     EVAPPWYIYSSTPVPKGPMPTKFEVNEMKGFEIVGSMRDSEEGETKFDKAFKVDVKVF
                     HDTATFIQTIRKLTDSTIVVSGLLSWQTCNGKECLIDETDVKFEIKGVEPSPEPPVKS
                     NDESIWMFLLIAFAAGLGGVFTPCVFPMIPMTVGFFISGSGNRKTGIIKGITFGLSVT
                     LIYTLVGLIVAAFQSTDTTDVLGTHWIPNALFALLFIIFAISFLGAFEITLPGGLANK
                     ADANADKGGIIASFFVALAMVIVSFSCTGPFVGSILAAAVSSGVTLKPILGMFVFGLA
                     FSLPFVVFSFSPALMKKMPKSGGWLNMVKVIFAFILLAFSMKYLSAVDAWFGWGIISR
                     PLVISVWIVIAALLGFYLLGKIKTPHDSEVTHVGAGRMISAIISFTFAIFLIPGLLGA
                     PLTYLSALLPPPSANTFTIYASTARNESFQAFTKEHDVNIGGSGPCEPVKYADTEHPA
                     PYGLTAYYDISQAIECSKSLKKPVLLAFKSATCSGCKLMEATVWSDPEVQKILREDLV
                     LATLYIDNKTELPKEEQVLSKIDGKVKNTLGRKIRDYQVSHFGVASQPYYVIVDQNEI
                     SLVQPIGECSKEEFLSFLKNGVEKYRSNNLK"
     gene            9071..10609
                     /locus_tag="P5293_02235"
     CDS             9071..10609
                     /locus_tag="P5293_02235"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRR48758.1"
                     /translation="MTQEDLFKSLIAHAKEYGFIFQSSEIYDGLSAVYDYGPLGVELK
                     NNIKNYWWSAMVRLNENIVGIDSAIFMHPKIWEASGHVGAFNDPLIDNKDSKKRYRAD
                     NLLEDYIDKIEEKIEKEVEKGRKKFGESFDESKFVSTNPNIVRHKAKIDEVAKRMNDA
                     LKSNDLIALKQLILDCEIVCPISGTRNWTDVRQFNLMFSTQLGNTNDDTNIIYLRPET
                     AQGIFVNFLNVQKTSRLKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     EELEWFKFWKDKRLKWHKAIGLGNDKYRFHDHEKLAHYANAATDIEFNFPFGFKELEG
                     IHSRTDFDLCQHQKYSGKKIQYFDSETGESYVPYVIETSIGLDRTFLAIYSSCYCEEK
                     LADGEERVVLRFPAFIAPVKLAVLPLIKKDGLPEMARDIMNDLKFDFSCQYDEKDSIG
                     RRYRRQDAIGTPYCITVDHQSLEDNTVTLRYRDSMEQERIEISRLRNTIAEKVSLRKL
                     LETI"
     gene            10622..11602
                     /locus_tag="P5293_02240"
     CDS             10622..11602
                     /locus_tag="P5293_02240"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HRR48759.1"
                     /translation="MENISVNYMGMSLKSPVIAASSGLTTQLDKIESFEKAEVGAIVV
                     KSLFEEQIVNEADFLKSRSSDYPESADYLHHYIRQYSIEKYLNLLKEIKSKISVPVIA
                     SINCYSKGEWMSYAKEIEQTGVDGIELNIYSLPLNVHKTSTEIEKDYLEVVGNVTKMI
                     KIPVAVKIAGYVTNLPGFVYNLKAYGAKGVVMFNRFYSPDIDINKLKIIPADAFSNES
                     ESLRELRWMGIVSALVPGIDLAASTGIHTGETAIKQILAGASAVQMCTALYKRGADAI
                     SEANQEILLFMNRHGFNSIRDFKGALNYSNIEDPRKFERVQFMKNFGGKV"
     gene            11813..12196
                     /locus_tag="P5293_02245"
     CDS             11813..12196
                     /locus_tag="P5293_02245"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR48760.1"
                     /translation="MAKVIFKSYKENDSVLLPPSLGDIVPQTHPARIVSSVIDHLDIS
                     SIESKYKGGGTSSYNPRMLIKVLVYGYMCNTFSGRKIERQLKENIIYMWLSGYSTPDF
                     RTLNLFRSQRLNGDFESIFTLELLT"
     gene            12239..12538
                     /locus_tag="P5293_02250"
     CDS             12239..12538
                     /locus_tag="P5293_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR48761.1"
                     /translation="MRKQRMHFDKAFKENAVKLSLERKNVSELAQELGIEAFLLYRWR
                     KEFQEKGDSSFPGNGNQSLSEENKRIVELEKRLNDAEIERDILKKALGIISKSGR"
     gene            12535..13428
                     /locus_tag="P5293_02255"
     CDS             12535..13428
                     /locus_tag="P5293_02255"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRR48762.1"
                     /translation="MIFQFIKDYNSKRWTIEVMCRVLDASKSSYYRWFKEPVGKRKQN
                     YMELDKKIKQAYFTAKGRNGSPRLTKDLLACGIVVSRTTVASHMKQMGLCSKLSKRHK
                     VTTNSSHDFEVAPNLLNRVFIHEEPVKACVSDLTYISCLDGFLYLTCVLDLFDRKLIS
                     WSMSDNMSASKTVIPAIRMVNRNGAFPKNMIFHSDKGSQYACKQTVNILKSLNAKQSM
                     SRKGNCWDNAVAESFFKSFKTELIYGKRLITREQMRLLVFEYIESWYNHKRKFSALKN
                     LTIDEFWEQYNIKKQIIKNVA"
     gene            13418..13720
                     /locus_tag="P5293_02260"
     CDS             13418..13720
                     /locus_tag="P5293_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR48763.1"
                     /translation="MLLNFLSQISWQIQLIRELLTSERGLMHRSKRPVEPEAVFGQIK
                     YDSGFKRFMLKSLPKVSVEFGLVALAHNLRKYASNLHSILTKKYLKNRFFFKDLMV"
     gene            complement(13828..15450)
                     /locus_tag="P5293_02265"
     CDS             complement(13828..15450)
                     /locus_tag="P5293_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48764.1"
                     /translation="MKNKLIYTLAIVAIAFNSETLLAQKNKVSDKCLYYFDKLEKSAP
                     WIVSENGAGLVFNSAQNFSSFGAFYGYDNGEYKNYYDPSEYNTFGISTKSYVKVKDVY
                     YFGELIYDYGIKQNQGWLGTIYPNTTNDPIVDSIPGKVLREDYYLNGKIGYPLSDRVS
                     IGLGVNYHTATAAKRTDGRNRNTLSSFNISPGIVFNNGILSLGLNLSYKHDVERVNYE
                     YLGDETGKAIYYMEGLFFYYKTGITNTTVLNRGYFKDYLGGALQVELRKNNVKFFNQL
                     KVDYNNEDDYEGTGLSKRYAYTDGLKYDYSGTLSVITKTIDNYLKLGFTNNQQFSYNV
                     ITNYELVPNEISTWNYFEYGKTLRYMVDDTRYGAEYKGFLKRQDGCKWDFSWIFTLGA
                     NYNKVKKDYKIYPATYHQDYENTEIYANILKNFRIKDIALIDVDVEGSYVNGDGTMLE
                     YKNPITTGSLLLNNSLLQHDFAYRTADRAKIGGGIKYTHILNATKGITAFCKASYTYQ
                     YLIDDGGLDNILDGVLPGTYKSFVNVSIGLNF"
     gene            complement(15461..16705)
                     /locus_tag="P5293_02270"
     CDS             complement(15461..16705)
                     /locus_tag="P5293_02270"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HRR48765.1"
                     /translation="MKNIKTIIFLIASGISITGCMKDLKNDDFSDDAISATITVTVNM
                     PAGYNYPTEGLPVYFVDPSSGLKFCDTTDAAGVATIRVPHGAYVATTETKHSVSGGVI
                     YIFNGTSEKIRVTPKDPRSVSSTINLNVSKAGQIVLKELYYGGCMNPATGKSYSKDTY
                     FILYNNSDEVAYLDSLCLGVADPYNAPTSGKTSNWVKPGTTELRDSVPIASFGWMFPG
                     NGTSYPLQPGEQAVVSLNAIDHTTTSITSVNLGKPGYWAAYDPLLTRGQSTPNAGVNL
                     MQGFWKVGTATSWVVSSLSPAVILYTMGGKTPEQFVADTYTWNPGYATNRNFDCLMVD
                     KNLVIDGVECFRNDTDSKRLRPEIDNGYTKTDGSGQGQSVHRKIDAQATIAAGGRIVY
                     MDTNNSSNDFEKRATASLVGVN"
     gene            complement(16718..>18028)
                     /locus_tag="P5293_02275"
     CDS             complement(16718..>18028)
                     /locus_tag="P5293_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR48766.1"
                     /translation="MFIEGYDYDPTIDPRFNAKYKIIDKRKKEGVLRSLAIRGGWGIM
                     QKLPSISILYPAPSYLDNCLFQYRNSSTGESLAVIQTSVIDERLPYNLEPAKTKNAEF
                     GIDFNILGVDAQITYFNESLTNGISSNNFYVPQTYDYYNTVTSQTASPKFENGRVWVK
                     NEQGVYEQLGYKTNTEYKHYSRPDNRAKSNKWGIEYDINFGKISPISTSVIVNGSYIR
                     TENEYNGQYYSYTGSADPIVPSQKFEYVSIWSGDNSMTKGTGRERFSTNISLITHIPS
                     IRMIISLVTQCIWVYNTWNLFDEGNIYSIDANGNRVFGDYSNQNNTVILYRDPLYYMD
                     FAGNIRPFSDYYTTTDQNLKTRLGMLRKATDVSYYFNKQGYDPFFMANIRVTKEIGKL
                     ASLSFYANNFTNSTPIMKDKARPNAVGTRVNPGIYFGAELKLTF"
BASE COUNT         5580 a         3171 c         3462 g         5815 t
ORIGIN      
        1 atttcactgt tattaataag ctcttcattg gttgtgaaag aaattttatt ttttgtcgcc
       61 catgttctga gattttcgaa attagggact ataacagctg aagcaaattt ttgattttct
      121 ccaacgacca tacaattttc aatatatgaa gattcattca acatcatctc tatcacttga
      181 ggtgcaatat attttcctga tgataatttg aatatttcct tttttctatc ggtaatcttt
      241 aaaaatattc cgtctattat cgttccgata tctcctgtat ggaagaaccc ttcttcatca
      301 ataacgcttt ttgtatattc agggtctttg tagtagccaa gcataacatg cggccctctg
      361 gttaaaattt ctccatcttc ggcaattttc aaatctgtcc ctactgtagg tttaccaaca
      421 gttcctattt tgataatcat ttcttttgga ttatttacgg cgattattgg ggacgtttct
      481 gtcatgccgt agccttcaaa tatatacatt tttgcagcag tgaagagtct tacaatcttt
      541 tcctgaatag ctgatccacc cgataccaca agcatctctt tgccacctaa cttttctcgc
      601 catttgctgt agacaagttt gtctgccaaa ccatacattg ttttgtataa aaccccattt
      661 ttggtataat catatttatt tgcaatatta aatgcccatt tatatataag ttttgcaata
      721 cctttaagat cttttcctgc agattcgaat ttactataca tgacttcaag aactctcggt
      781 acagcgcaaa aaccatctgc gtggcagtca gcaagatctg cggcaatagt ccctaaactc
      841 tctgcgtaat aggtgcttat gccgagatac tgatagtcat aattcatgct tctttcatat
      901 acgtgacata atggtaaaaa actaagcatc ttgtgattat ggtctcttat ctgttgtact
      961 gctgaaccaa taaatgtaaa aactaaattt ttatgtgaaa gcattactcc ttttggagta
     1021 ccggttgtcc ctgaagtata gataattgtt accaaatcat tttcatcaat agtccttttg
     1081 ttttcttcta tttgtgaaaa gtatctctgg gaattatttt tgccaagagt aaatagctca
     1141 gaaagacttt ttatgcccgg tacttcatct attgaataga catccggagg cgtattcatt
     1201 gatgaaacgg caggttttat tcttttataa attgattcat tcccgacaat aataatctta
     1261 gcatcactat ggttgaatat gtgttgatat tcatcattgc ccagtgtggg atagaccgga
     1321 acgtgaacaa gatgtgccag agaaagtccc atatctatga aattccactc ttgacgattg
     1381 ttgcatattg ttattacttt gtcaaatggt ttaagaccaa gtgaaaggaa agcgtatgct
     1441 agaatatgcg atttctcctt gtattcttga gaagaacagt ttatccagat accattgtgt
     1501 tttctactga atatatcatc ttttggataa agtgtaacca acctttccaa taaatcgaat
     1561 gtcctagtta cttccatgta tttaatagtg ttaactcttt tttaagcttt ggatttcaat
     1621 attgattgtt taatatcact tacaaagttg tctacatctt ctttggcagt gttaaatgag
     1681 cacatccacc tgacttcgtt agtggcttca ttccatacgc tgaaattgtg tgtggacatg
     1741 agatcatcag atagaaattc aggaataatt gcaaagactg catttgtcat tacaggcctg
     1801 ctgattttaa cttcattgat gccttgtaat ttatattcaa ggtattttgc catttcattg
     1861 gaatgtcttg caagttttaa gtttagatca tctttcagat aagcaataaa ctgtgctgct
     1921 ataaatcggt tttttgaata taactgagta ttttgcttgc gtacataagg aaaatcttca
     1981 gcaaaaggtt ttttgaagaa aattactgct tcacctatta gtagaccatt tttagtgcct
     2041 ccaaatgaaa gagcatccac accaagatct gcagtaatag ctttgatcga taatcctaga
     2101 gcagcagctg cattagatat tcttgatcca tccatgtgca aaaaacagtc atatgaatgc
     2161 atcaaatcag caagagacct tatttctcca ggagtgtaaa gtgtgcccaa ctctgttggt
     2221 tgtgaaatag ataagacttt gacttgtgag tgatgttgaa ctccaaaatt tactagctct
     2281 tttcttactt cttcagggtt tactttcccg tcagttgaat gaagcggaat aagtttgcag
     2341 cctgttgcct tttcaggagc gccacactcg tctacattaa tatgagcagt gtccgggcag
     2401 agaatggaat tatgtgattt tgtaagtgtt tttaatgcag taacgttagc tcccgttcca
     2461 ttaaatacaa aaaatggctc agcattttca ccaagcttct ctttgaaaat tctaattgct
     2521 tttgcagtgg tttcatcatc tccgtaagca acagagaatc cctcatttgc ctcaattatg
     2581 gcttccatta tttcaggtgc tactcctgaa tgattatcgc ttccgaaact gtgtttcatt
     2641 aaacctccgt attaattgtt taacaagtgt aaatataaca aaaacccaca gagatattca
     2701 ctgtgggttt catattgctg aaattctgac tacaaaattg ccttgtaaga ttcggcatcg
     2761 agaagagagt taagttctga cgggtcagaa atttcaatct taatcatcca tcctttgccg
     2821 tatggatctg tatttactag ttcaggagca tcttccagtt caggatttat ctcaattatt
     2881 ttgccagaaa caggcatcaa tgcgtcggcc actgttttaa cagcctcgat ggctccgaaa
     2941 atctcagatg catcaagtgt ttcgccttct gtctggatat cgacgaaaac tatgtctcca
     3001 agctgatttt gtgcaaaatc ggtgatgcct actgtggcaa tattaccgtc aactttaacc
     3061 cattcgtggt ctttactgta cttaagattt gcaggaatat tcataactgt cttttattgt
     3121 tgtttatttt gacaaatgta caaaaagatt aaactctttt caatgcctct tttatgagtg
     3181 tttcaagttt ttggttagga ttttctttta gtatcgagtc tatcactttt tcgacattct
     3241 gtttagggaa acctaaaagg acaagagcac ttgatgcctc ttctcgaatt tgagagacag
     3301 gactcacaga aataacgccg gtttttcccg aaggagttcc tttaatgatt ttatctttta
     3361 agtcgattat tattttttga gctgttttta atcctatccc ttttacactt ttaattttat
     3421 ttatatcatc gctaatgatt gcatttctaa gttcttcagt agaaagagat gaaagcatca
     3481 gtttggcagt atttggacca acgcctgtaa cgttaataag taaagagaat atataacgtt
     3541 cctctttgtc aaaaaagccg tagagcagtt cagtatcttc tcttatgtga tggtataaat
     3601 agagtttgca ttctgtgtca ggagtcaatt gagagaatgt ctggagagag ataagaattc
     3661 tatagccaaa attatttgtt tcaatcacaa cttcagtagg atttaactca acaactttcc
     3721 cttttatgta gtcaaacatt tcgtttcagt aaacatttaa ggcaaaatta tgttttttaa
     3781 aaaacaaatc gaaactttgt ttactatttt tgtaccaaaa gatttttatg gagttgatca
     3841 aggatgtatt ggatataaga agaaatttcc ctgctctgga acagagtgtc aatgggaaac
     3901 cactggttta ttttgacaat ggagctacct ctcagaagcc tcgttctgtt attgaacttg
     3961 tcaacagaat gaattcgagc gttaacggta acattcacag agctgtgcat gaactcagtg
     4021 caagggcaac tgaaatatat gaaaattcga gagaggagat tcgtaaattt atcaatgcag
     4081 tatcgagaga agaggttatt tttacttcag gtacaacagc atcaattaat cttgttgcga
     4141 actcattttc cagggtgttt atagataagg gagatgttgt tttgattact gaaggcgaac
     4201 atcattctaa tattgtccca tggcaaatgg cttgtgagag gtatggggcc gaattgagag
     4261 tccttcccgt tgacgataac ggctgctgga ggatggaaca attggactcc cttctcatgg
     4321 atggtaaggt taagatagca tcggttgccc acgtttcgaa cgttttaggt gtagttaatc
     4381 cagttaatac tttaattgaa atatgccata aggcaggagt tcctgtactt ctcgatggag
     4441 ctcagggtat tattcattta aatgtagatg ttcagaaact tgactgtgac ttttatgtat
     4501 tttccggcca taagcttttt ggaccaacag gcataggaat attgtacggc aaaaaagatt
     4561 ttttaaatgc aataccgccc tggatgggcg gtggagatat ggtgggcaga gtgtcatttg
     4621 aaaagacaac ttatgccgag ctccctttaa aatttgaggc aggtactccc aactttatag
     4681 gtgctgcggg tctcggggaa gcaataagat ttattaataa tattgactca gaatttgtta
     4741 aggaacatga aagacagttg gttgagagga tgatgaatgg tctttcttct gttggatctc
     4801 tcataatata cggtacatct gacagcaaag ttccgctttt ttcgttcaat gtgcccggaa
     4861 ttcatccaac tgatatggct atgctgatgg ataaaatggg aatagccttg agatcgggta
     4921 tgatgtgttc agagccatta atggatcatt ttggtgtatc cggaatgtta agagcgtctt
     4981 tgacaattta caacaatctt gaagaagtcg attattttat cgaatcattg caaaaagcaa
     5041 taagaatgtt gtcttaatag aataacataa aatcgcatgg caaatgacaa ttaaagaggt
     5101 tgaacaacaa ataattgatg aattttctgt ctttactgac tggttggata aatatgagta
     5161 cttgattgaa cttggcaagt cacttccctt aatagatgag aagaaaaaaa tagatgaaaa
     5221 cttaataaga ggttgccaat caaaagtttg gctggcagct gattatgtag atggaaaaat
     5281 ttatttcaca gcagatagtg atgctattat tactaaggga attatatctc ttctgataag
     5341 agtcttttct ggaagaacgc cacaagaaat tctggatgcg gatcttaatt ttattaaaga
     5401 gattggtctg caggacaacc tttcaccaac acggtccaat ggtttggtat ctatgataaa
     5461 gcaaattaaa ctatatgcac tcgccttttc ggccaataaa tgatttttac tatggcagat
     5521 acttcagttg aaaagaatat tgtattagca ctcaaacagg tatatgatcc tgaaataccc
     5581 gtcaacgttt atgaccttgg attgatttat gatttaaaag tcgatgacag tggtaaggtt
     5641 tttataaaga tgacgcttac tgctcccaat tgtcctataa tggatgatct tctacaagac
     5701 attcatgatg ctgtaatgga tgttccttct gttaccgatg tcgctattga attggttttt
     5761 gatccacctt gggacaaaag tagaatgagt caggaagctc aactcgaatt aggttttctt
     5821 taaaagatgg aaaaagtaat actgtcactt ggcagtaata aaggagatag attgaaatac
     5881 ttaagaagtg caattgatag aattggtgat tgtatttata aaactcctaa aatttctcag
     5941 atttacgaga gtgagccatg gggttttaat tcaacggtct ggtttcttaa tattgcaatg
     6001 ctgcttgaaa cggatttgtc tccaatcgag ttgctggatg aattaaatat tatcgaaaaa
     6061 gagctcggga gagtgagaaa gagtaatgct tctgattatg aatcccgggt tatagatata
     6121 gatataattt tttttggtga tagagtgctt gatcaagcgc ggcttataat tcctcaccct
     6181 tttgctcatt taagaagatt cgttctttta ccggttcttg atatttctcc ccattatatt
     6241 catcctgtct taaataaaac aataagagaa cttgttgaga gttgccctga caagtctgaa
     6301 ttgagactgt tttgtcatga aatttaaccg ctatagaacc aatatcagat ttgtttgttt
     6361 tatgtattat taaattaaca aaagactaaa aaaattgaga aatgaatatt atagaaggat
     6421 tacagtggcg ttatgcaaca aagcgatttg atacagataa aaagctaaat gaagagcaaa
     6481 tagagtattt gaaacaggca gtgagtttga taccgtcttc atacggatta caaccatata
     6541 aagcacttat tgtagaagac cctgagatta gaaaaaagct tagtgaggct gcttttggac
     6601 aacccgcaat tactgaagcc tcgcatctct ttatttttgc aaacttcaca gaatatgggc
     6661 ccgataaagt cgaatcattt ttgagactaa gtgccgaaat taataattat cccgagggca
     6721 gttctgatac atacagagat atgcttaacg gtatgatagg ggcgatgact ccggagcaac
     6781 tttcaaactg gacagcaaag caggcttata tagcgcttgg agcacttatt tgtgctgcct
     6841 ctgaacagca tattgacatt tgtcctatgg agggttttga caaatctcaa tttgatgata
     6901 ttttggaact cagacagaaa ggccttactg ctgcatgtat aggagcagtc ggttatagat
     6961 cacctcaaga taggcatcaa tttctcaaaa aagttagaaa gcctttagat cagttatttg
     7021 aggttatttg atctgtattt ttcaacccca tttttgagaa aactcaggaa ttcctcttta
     7081 gagcattcgc ctataggctg aacaagagaa atttcatttt gatctacaat tacgtaataa
     7141 ggttgtgatg caactccaaa atgtgacacc tgatagtccc ttatttttct gccaagtgta
     7201 tttttgactt tcccatcaat ttttgataag acttgttctt ctttaggaag ttcagtttta
     7261 ttgtcaatgt acagagtggc aagaacaaga tcttctctga gaattttttg gacttctgga
     7321 tcgctccata cagttgcttc cataagtttg caaccactgc atgttgctga cttaaatgca
     7381 agcaatactg gtttttttaa acttttagag cattcgatag cctgagatat gtcataatag
     7441 gcagttaatc catatggcgc cggatgttca gtatctgcgt attttacagg ttcacatggg
     7501 ccactcccgc caatattaac atcatgttct tttgtaaaag cctgaaaaga ttcatttcga
     7561 gcagtagagg catatattgt aaatgtattt gcacttggag gaggaagtaa agcagacaag
     7621 taagtcagtg gagcacccag aagtccggga attaagaaaa ttgcaaaagt gaaggagata
     7681 attgcagaaa tcattcttcc tgcacctaca tgtgtaactt ctgaatcatg cggagttttt
     7741 atcttaccga gcagataaaa tcccagcaga gctgcaataa caatccatac tgatatcacc
     7801 agtggccttg aaattattcc ccatccaaac caggcatcca cagctgagag atatttcatg
     7861 ctaaaggcaa gcaaaataaa agcgaatata actttaacca tatttaacca acctccagat
     7921 ttaggcatct ttttcattaa agcaggggaa aaagagaata ctacaaatgg cagggaaaaa
     7981 gcaagtccaa acacaaacat cccgagtatc ggcttaagag taactccact tgatacagca
     8041 gctgctagta tagatcccac aaatggtcct gtgcaggaaa aagagacgat aaccatagcg
     8101 agagccacaa aaaatgaggc aataattcca cctttatcag cgtttgcatc agctttatta
     8161 gcaagaccgc ccggcaaggt tatttcaaat gcccctaaaa atgagatggc aaaaatgata
     8221 aaaaggaggg caaaaagtgc attaggaatc cagtgagttc caaggacatc tgttgtgtca
     8281 gtactttgaa atgcagctac aataagccct acaagtgtat atataagcgt taccgaaagt
     8341 ccaaaagtta ttcctttaat tatacctgtt tttcgattac cggaaccact aatgaaaaat
     8401 cccacagtca taggaatcat agggaatacg catggagtaa aaactccccc aagtccagca
     8461 gcaaatgcaa ttaaaagaaa catccaaatg ctttcatcat tgctttttac agggggctct
     8521 ggagatggtt ctaccccttt tatctcaaat ttaacgtcag tttcatctat cagacactcc
     8581 tttccattac atgtttgcca tgacaggagt ccgcttacaa caattgttga atctgtaagc
     8641 tttctgattg tctgaataaa tgttgcagtg tcatgaaaga cctttacatc tactttgaac
     8701 gccttgtcaa acttggtttc gccttcttca ctatctctca ttgaacctac aatctcaaat
     8761 cctttcatct cattaacctc aaattttgtg ggcataggac ctttgggaac cggggtactg
     8821 ctatagatat accatggtgg tgcaacttct gctttaaaga ttaaatcaac agtaccatct
     8881 tcattatctt tctgtgtaaa tgtccaagtt gtagtagaag tttgagcacc aattaggttg
     8941 atagaaagga tggaaaataa taaaagaata agtcttttca tgcgatgtat aatttttaag
     9001 aatttcaaaa ttatcaaaaa tcctaaaatt gcactaattt tgtaaattac aaagttgaca
     9061 aattaacaag atgacacagg aggatttatt taagagcctt atagctcatg ccaaagagta
     9121 tggatttatt tttcaatcga gtgaaattta tgatggattg agtgcggtat atgattatgg
     9181 tccacttggc gttgaactta agaataatat taaaaattat tggtggagcg caatggtccg
     9241 cctcaatgaa aatatagttg ggatagattc ggcaatcttt atgcatccaa aaatctggga
     9301 agcgtcagga cacgttggag cctttaatga tccattaatc gataataaag attctaaaaa
     9361 aagatatcgt gctgacaatc ttttggaaga ttatattgac aagattgagg agaaaataga
     9421 gaaggaagtt gagaagggaa gaaagaagtt tggggagtcg tttgatgaat caaaatttgt
     9481 atcaaccaat cctaatatag taagacataa ggctaagatt gatgaagtag caaaaaggat
     9541 gaatgatgct cttaagtcta atgatttgat tgctctcaag cagttgatac ttgattgtga
     9601 aattgtctgc cctatttcag gaacaagaaa ctggacagat gtacgtcagt ttaatctaat
     9661 gttttcaact caactaggga atactaacga tgatactaat attatatatc tcaggcctga
     9721 aacagcccag ggtatttttg tgaattttct caacgttcaa aagacttcga ggctaaaaat
     9781 accatttggt attgctcaaa ttggtaaagc gttcagaaac gagattgtgg caaggcagtt
     9841 tattttcagg atgagagagt ttgaacagat ggaaatgcag ttttttgttc gtcccggaga
     9901 ggagttggag tggtttaaat tttggaagga taagcgtctt aaatggcata aggctattgg
     9961 ccttggaaat gataaatatc gttttcatga tcacgaaaaa ctggctcatt acgcaaatgc
    10021 ggccacagac attgaattta acttcccttt tggttttaag gaacttgaag gtatccattc
    10081 acggacagac tttgatttgt gtcaacacca gaaatattcg gggaaaaaga tacagtactt
    10141 tgattctgag acaggagaaa gctatgtccc gtatgttatt gaaacatcaa tcggccttga
    10201 tcgaacattt cttgccattt attcttcatg ttattgtgag gaaaaacttg ctgatggaga
    10261 agagagagtc gttttgagat ttccggcctt cattgcacct gtcaagcttg cagttttgcc
    10321 cttgattaaa aaagatgggt tgcctgaaat ggcaagggat attatgaacg atctaaagtt
    10381 tgatttcagt tgtcaatacg acgaaaaaga cagtataggc aggcgttacc gccgtcaaga
    10441 cgcaatcggc acgccatatt gtattacagt tgaccatcaa agtctggaag ataatacagt
    10501 gaccctcagg tatagagatt caatggaaca ggaaaggatc gaaatttcta ggctaagaaa
    10561 tactatcgca gaaaaggtta gtcttagaaa attattggaa acaatttaaa atctgaaaat
    10621 tatggaaaat atttcagtaa attatatggg gatgtcgctt aagtcacctg ttattgcggc
    10681 aagttcaggt ttgactacac agttagataa aattgaatct tttgagaagg cggaagtagg
    10741 agcaattgta gtcaaatctc tttttgaaga gcaaattgtc aacgaggcag actttcttaa
    10801 atcaagaagc agtgattatc ctgagtctgc agattatttg catcattaca tacgtcaata
    10861 ttcaattgag aaatatttaa atcttcttaa agaaatcaag tccaaaattt ctgtcccggt
    10921 tattgcaagt atcaattgtt attcaaaagg ggagtggatg tcctatgcaa aggagattga
    10981 gcaaacaggc gttgatggga ttgagcttaa tatctattct ttgcccttga atgtacataa
    11041 aacatctaca gagattgaaa aggattatct tgaggttgtt ggaaatgtta cgaaaatgat
    11101 taaaatccct gttgccgtaa aaattgcagg atatgtaacg aatcttcccg gttttgtcta
    11161 taatcttaag gcgtatggtg ccaagggagt agtgatgttc aacaggtttt attctcctga
    11221 catagatatt aacaaactta aaattattcc agctgatgct ttttcaaatg agtcagagtc
    11281 tttacgtgaa ttaagatgga tggggattgt atctgcactt gttcccggga tagatcttgc
    11341 cgcaagtaca ggtattcata caggtgaaac agcaattaaa cagattcttg caggggcttc
    11401 agctgtgcag atgtgtacag ctctttacaa aaggggtgca gatgctattt ctgaggcaaa
    11461 tcaggaaatt cttcttttta tgaataggca tggatttaac agtatcagag attttaaagg
    11521 ggcgttgaat tactcaaata ttgaggatcc aaggaagttc gaaagagttc agtttatgaa
    11581 aaattttggc gggaaagttt gatttcaagt ttaataaaat taaagagggt gactaaaaag
    11641 taaagtcgcc ctctttttca tatatcgcgg cgccaaatcc ggaagagaaa aagatttttt
    11701 cgtaaaataa agggttaatt acttgatata aaagtaaata acccttgctt gtttcaatta
    11761 aattatgtat ctttatatcg acaaaaacat aagaacatta aaaagaacaa gcatggcaaa
    11821 ggtaatcttt aaatcttaca aagagaacga ttcggtactc cttcctcctt cattgggaga
    11881 tatagtacct caaacacatc ctgcaagaat agttagcagc gttatcgacc atcttgacat
    11941 cagttcaata gaatccaagt acaagggcgg cggtacatca agctacaatc caaggatgct
    12001 tatcaaggtt ctcgtttatg gatatatgtg caatacattt tcagggagga agatagagag
    12061 acaactaaag gaaaatatca tctatatgtg gctttcagga tactctaccc cagacttcag
    12121 gacattgaat ctattcaggt cccaaaggct taatggcgac tttgagagca tctttacact
    12181 ggaattacta acttagttag gacacagggt taagcacaaa agtttatttt tgtgtattat
    12241 gagaaaacaa cgtatgcatt ttgacaaagc gtttaaagaa aatgctgtca aactaagttt
    12301 agagcgcaaa aatgtttcgg aacttgcgca agaattagga atagaggcat ttttgctata
    12361 tagatggcgt aaggaattcc aagagaaagg agattcaagt tttcccggga acggcaacca
    12421 gtctctttcc gaagagaaca agagaatcgt agagcttgaa aagcgtttaa atgatgctga
    12481 gatagaacgc gatatattaa aaaaagcttt aggcatcatc tccaagagcg gtcgttgatc
    12541 tttcagttta tcaaagacta caactctaag cgatggacga ttgaggtgat gtgcagagtt
    12601 ctggatgcat ccaaaagctc ctattatcgt tggtttaaag agcctgtggg caagcgcaaa
    12661 cagaattata tggaacttga taaaaagatt aaacaagcat actttacagc caagggtcgt
    12721 aatggaagtc cacgtcttac aaaagacttg ctggcttgcg gtattgtcgt ttccagaacg
    12781 actgttgcca gccatatgaa acaaatggga ttgtgtagca aactttctaa acgacacaag
    12841 gttaccacca actcctctca tgattttgag gtagccccga accttctcaa tagggttttt
    12901 atacatgaag agcctgttaa agcttgtgtg tctgacctga catatatctc ttgtctggat
    12961 ggattccttt atctgacttg tgtcttggat ctttttgacc gtaaactcat tagttggtcg
    13021 atgagtgata atatgagtgc atctaaaaca gttatcccgg ctataaggat ggtcaatagg
    13081 aacggggctt ttcctaaaaa tatgattttt cattctgaca agggttctca atatgcctgc
    13141 aagcaaacag tcaatatctt gaaatctctt aatgctaagc agagcatgag tcgtaaaggt
    13201 aattgctggg ataacgcagt agcagaaagc tttttcaaat ccttcaagac agaattgatt
    13261 tatggtaaaa ggcttataac tagagaacaa atgcgcttgt tggtatttga gtatattgaa
    13321 tcctggtata accataagag aaaattctca gctttaaaga atttgactat tgatgaattt
    13381 tgggaacagt ataatattaa aaaacagatt attaaaaatg ttgcttaact ttttgtctca
    13441 aataagttgg caaattcagc taataaggga gttgttgacc agcgagcgag gacttatgca
    13501 cagaagtaaa agaccagttg agcccgaagc tgtattcggt cagatcaaat acgattctgg
    13561 ctttaaacga tttatgttaa aatcgctccc taaggtatca gtcgagttcg gactagttgc
    13621 attagcccat aaccttagaa aatatgctag caatctgcac tctattttga cgaaaaaata
    13681 cctaaaaaac cgctttttct tcaaagatct aatggtttaa tagagataat catcttccct
    13741 tcgaaaaaac aagttgcctg aaaaaagaaa agagggaaac caaaaatctc tttttagtca
    13801 ccctctttaa tttaacatac atttatatta gaaattaagt ccaatactca catttacaaa
    13861 cgatttatat gttccgggca gaactccgtc gagtatgttg tcaagacctc catcatcaat
    13921 taaatattgg tatgtgtatg aagctttgca aaaagctgtt atgccctttg ttgcattgag
    13981 aatgtgagtg tacttgattc cccctccaat ttttgccctg tctgctgtcc tgtatgcaaa
    14041 atcatgttga agaagtgaat tgttaagcaa tagactaccg gttgttatag ggttcttata
    14101 ttcaagcatt gttccgtcac cattgacata acttccttca acatcaacat ctatcaaagc
    14161 tatgtctttt attctgaagt ttttcagaat gttggcgtag atttctgtgt tttcataatc
    14221 ctgatgatag gtggctgggt atattttata atcctttttg actttattgt aattagcacc
    14281 caatgtgaaa atccatgaga aatcccattt gcagccatcc tggcgcttta aaaatccctt
    14341 atattcagca ccgtatcttg tgtcatctac catatatcta agagtcttgc catactcgaa
    14401 atagttccag gtgctaattt cgtttggtac taactcataa ttggtaatga cattgtaact
    14461 aaactgttga ttatttgtga agcccagctt cagatagttg tcaatagttt ttgtaataac
    14521 agagagagtt cctgaatagt catatttaag tccatcagta tatgcgtatc tttttgaaag
    14581 gcccgtaccc tcataatcat cttcattgtt gtagtcaact ttgagttgat tgaaaaattt
    14641 cacgttattc tttctcaact ctacttgcag tgcaccacct aaataatctt tgaagtaccc
    14701 tctattgagg acagtagtat ttgtaatccc agtcttatag tagaaaaata ggccttccat
    14761 ataataaata gctttacctg tttcatcacc aagatattca taattgactc tctctacatc
    14821 atgtttgtat gaaagattaa ggccgagact taatattcca ttattaaaga caattccggg
    14881 tgagatgtta aaactggaga gagtatttct atttcttcca tctgttcgct ttgcagcagt
    14941 agcagtatga taattaactc ctaagccaat agaaactcga tctgaaagtg gatacccaat
    15001 cttgccatta agataataat cttctctaag tactttccct gggatagagt caacaatagg
    15061 atcattcgtt gtatttgggt atatagttcc gagccatccc tgattttgtt ttatcccata
    15121 atcgtagata agctctccga agtaatagac gtccttaacc ttaacataag attttgttga
    15181 gattccgaaa gtattatatt cagaaggatc atagtaattc ttatactccc cattatcata
    15241 gccgtagaat gctccaaagg aagagaaatt ctgtgcagaa ttaaaaacta gtcctgcacc
    15301 attttcagac actatccatg gtgcactttt ttccaacttg tcaaagtaat acaggcattt
    15361 atccgatact ttgttttttt gtgccaaaag cgtttctgaa ttaaaagcaa tggccacaat
    15421 tgctaatgta tatattaatt tatttttcat cttctttagt ttaatttaca ccaaccagtg
    15481 aggctgttgc ccttttttca aaatcgttgg acgagttatt tgtgtccatg tatacaattc
    15541 ggccccctgc agctattgta gcttgagcat ctattttacg atgaacgctc tgcccctgtc
    15601 cggagccatc agtttttgtg taaccattat caatttctgg tctaagcctt ttggagtcag
    15661 tgtcattacg gaaacactct acaccatcaa taacaagatt cttgtctacc attaagcagt
    15721 caaagttcct gtttgttgca taaccagggt tccaagtgta agtatctgct acaaattgtt
    15781 caggagtttt gccgcccata gtgtataaaa ttactgcagg gctaagtgat gaaacaaccc
    15841 atgatgtagc agttcccact ttccagaatc cttgcattag attcactcct gcattaggtg
    15901 tagactgtcc tctagtaaga agtggatcat aagctgccca gtagccaggt tttcccagat
    15961 taacagatgt tatgctggtt gtagtatgat ctattgcatt tagcgaaaca accgcctgtt
    16021 ctccaggctg aagagggtaa gatgtgccat tgccagggaa catccatcca aaagaagcaa
    16081 ttggaacaga atctctaagt tctgtggtgc caggtttgac ccaatttgaa gtttttccgc
    16141 tggtaggagc attatatgga tccgcaacac ctaaacacag tgagtcgaga taggcaacct
    16201 catctgaatt attgtaaagg ataaaataag tatcttttga atagcttttg cctgtagcag
    16261 gattcataca tcctccgtaa tataattcct ttagcactat ctgacctgcc tttgaaacat
    16321 tgagattaat ggtggatgac acactccttg gatcctttgg tgttacacgt attttttcag
    16381 atgtcccatt aaagatgtaa ataacaccgc cggatacact gtgtttggtt tctgtagtgg
    16441 caacatatgc accgtgaggg actcttatag ttgccacacc tgcagcatct gtagtatcac
    16501 agaactttaa gcctgaactt ggatcaacaa aatatacagg aagcccttct gtcgggtaat
    16561 tatatccagc aggcatattc acagtcactg ttattgtcgc gctgatagca tcatctgaaa
    16621 aatcatcatt tttcagatct ttcatacatc cggtgattga tatacctgat gcgattagaa
    16681 atataatagt ttttatgttt ttcatgtcaa ctgtcaatta gaaggttaat tttaactcag
    16741 caccaaaata gatccctgga ttgactctag ttccaacagc atttggcctt gccttgtctt
    16801 tcataatcgg tgttgaattt gtaaagttgt ttgcatagaa ggaaagtgag gccagtttgc
    16861 ctatttcttt ggtaactcga atatttgcca taaagaatgg gtcataacct tgtttgttaa
    16921 aatagtaact aacatctgtg gctttcctta acattccaag tcttgttttt agattttgat
    16981 cggttgtagt gtaatagtca ctgaatggtc tgatattccc ggcaaaatcc atgtagtaca
    17041 ggggatcgcg ataaaggata acagtattgt tttgattgct gtaatctcca aaaactctat
    17101 taccatttgc gtcaattgaa taaatatttc cttcgtcaaa aagattccaa gtattataaa
    17161 cccaaataca ctgagtgaca agagatataa tcattctaat tgacggaatg tgagtaatga
    17221 ggctgatgtt tgtagagaat ctctccctgc ctgtaccttt agtcatggaa ttatctccgc
    17281 tccatataga tacatactca aatttctgag aaggtactat tggatctgct gagccagtat
    17341 aggaataata ttgtccgtta tattcattct ctgttcttat atatgagcca tttacaataa
    17401 cagatgtgct tattgggcta atcttaccaa aattaatatc atattctatg ccccatttat
    17461 ttgatttggc gcgattgtct ggtcttgaat aatgcttgta ttcagtgttg gtcttataac
    17521 caagctgttc atatacacct tgttcgtttt tcacccaaac tcttccattc tcaaattttg
    17581 gcgaggctgt ctgactggta acagtattat agtaatcata agtttgagga acataaaaat
    17641 tgtttgatga aataccatta gtcaaactct cgttaaaata tgtaatttga gcatctacac
    17701 caagaatgtt aaagtcaatt ccaaactccg cattttttgt ctttgcgggt tcaagattat
    17761 agggtagtct ttcgtcaatt acacttgtct gaataacagc tagagattct ccggttgaac
    17821 tatttctata ttggaaaagg cagttgtcaa ggtaacttgg tgcgggataa agtattgata
    17881 tagaaggcaa tttttgcata ataccccatc caccacgtat tgcgagagat cttaaaactc
    17941 cttctttttt tcttttatct attatcttgt atttggcatt aaaacgggga tctattgtag
    18001 ggtcataatc atatccttct atgaacat
//
LOCUS       DAOTQL010000018        17718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3113_length_17718_cov_5.203080, whole genome shotgun sequence.
ACCESSION   DAOTQL010000018 DAOTQL010000000
VERSION     DAOTQL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3113_length_17718_cov_5.203080"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2684)
                     /locus_tag="P5293_02280"
     CDS             complement(<1..2684)
                     /locus_tag="P5293_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR48767.1"
                     /translation="MKKKILFVVVLLLCNVAMAQIKVTGRVTSSDDGSPIPYANIQVK
                     GMKNIGATTDLDGRFSIENVSRDAILVVTYIGYATKEVPVQGRSEVNIELVSDVLNLD
                     EVMVVAYGTARKSTFTGAASVVKADAIKDVPTLSFENALSGKIAGMQITTNSGQAGSV
                     SSIRIRGIGSMNASNEPLYVIDGVPAISGSSGQMGDYLYTSNNVMSTLNPSDIESITV
                     LKDAAASALYGSRAANGVIVVTTKKGKEGKPVINFKASVGISPDWATDNYETATPEQQ
                     LELYYENFWNAGIYYQNKTPEQASANALAQMNKRFNKHGYTFTAPDNTVNSLTVGGDR
                     AGTFYNWDDVLFRTAVYQTYDMSVSGASDRSNYYSSLSYTKEQGRITINDYERVTGRV
                     NLNQKINRFIEFTTNINIARSEKSGFNDTRSTGANYFMQSRNLLWPLYWPTNVVTGQP
                     WTDRYGSYAYNPVYYNEQWENWTKMLKISANETLTIKFLPELIFKTVFSYDNTNTMDH
                     IYYSALHFNGSSTSGSVNEMSTNTSKLVSSSTLSYNKVFADKHTLGVLIGWEAEDNQT
                     YFQRSTGTNLPTSTLHTVSTAGKLDAAGYNWGNTMLSALSRIEYNYLSKYYFSASYRR
                     DGSSRLGENNRWGDFWSVAGSWKINSEKFMENLDYISNLRLRASYGVNGTLPTSNYGW
                     RSLTSYTNNYMQNPGGGVSTIADPDLSWETSYTYNFALEFGLFDQRLSGTIEYFDRTS
                     KDLLQDVPISLVTGFGSTLKNIGEINNHGIEIEISGDIIRKKDLRWSAGITASSIKSK
                     VNKLYGGQPITWYDPTGGDARAKFRYVEGGSTLALYGLEWAGVDDETGKNVWYLNNEA
                     TPDLTVNGRPATFSYSKASEVILGDVNPKLFGGINTDV"
     gene            complement(2879..5062)
                     /locus_tag="P5293_02285"
     CDS             complement(2879..5062)
                     /locus_tag="P5293_02285"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRR48768.1"
                     /translation="MNVVKKTIRLRDGREIEIETGKLAKQADGAVVVKQGKTMLLATV
                     VAAKEAKEDVDFMPLSVEYKEKYASAGRYPGGFLKREGRANNSEILVSRLIDRALRPL
                     FPDDYHAEVYVNVNLISADKEITPDALAGLAASAAIAVSDIPFNGPISEVRVARIGGQ
                     FVINPTFSELEKADIDIMVGATIDNILMVEGEMKEVSEKDMLEAIKFAHEEIKIHCKA
                     QMELMEEVGKTQKRAYCHETQDEDLRAAIKEFCYNRCYAVAKAGLGKQARTEALDNIK
                     AEFVATLSEEDAAAKKIMIDRYYHAVEKEAMRNLILDEGIRLDGRTTTQIRPIWCEVD
                     YLPATHGSAIFTRGETQSLTTVTLGTKLDMKEIDEVLVEGIEQFVLHYNFPPFSTGEA
                     KPYRGTGRREIGHGNLALRALKFVVPTGEENPYAVRVVSDILESNGSSSMATVCAGTL
                     ALMDAGVKIKKPVSGIAMGLITKDGKYAILSDILGDEDHLGDMDFKVAGTKDGITATQ
                     MDIKVDGLSYDILEKALEQARQGRLHIMGEMMKCISEPRADYKPHVPRIIQVIIPQEF
                     IGAVIGTGGKVIQEIQKVTGTTITISEENGTGVVEIFGEDKESMEKALGWIKGITAVP
                     QVGEVYKGKVVSILDFGAFVQILPGKEGLLHISEIDWKKIDKVEDELKIGDEVDVKLL
                     ETDPKTGKMRLSRKALLPKPEGYIEPKRKPRKEGDKKPFKSDKKN"
     gene            complement(5235..5507)
                     /gene="rpsO"
                     /locus_tag="P5293_02290"
     CDS             complement(5235..5507)
                     /gene="rpsO"
                     /locus_tag="P5293_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRR48769.1"
                     /translation="MNYLTPEKKQEIFGQYGKSNADTGSPESQVALFSYRIAHLTNHL
                     KNNKKDYVTQRSLLKLVGKRRRLLDYLKETDIERYRNIVKALNLRK"
     gene            5639..6370
                     /locus_tag="P5293_02295"
     CDS             5639..6370
                     /locus_tag="P5293_02295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRR48770.1"
                     /translation="MIKKYIHPRFGEIIITKKKGQRNIRLTVSLTKGVRVSIPYLTSF
                     SSAEKFIKAKEEWILSTIQKNRDKQLARIIPIGEGQKLQLVNSKVIYQQMSCREKSMP
                     GKILIRYLNEVTEVNLPQSCSKEAVLNATMVVIRRKAADYLPQRCKFLASENGFSYNK
                     LFLKNNKTNWGSCSNKGNINLNIQLIRLKAELIDFVILHELCHLKYHNHGTAFHNLLN
                     KLCQGREKELALQLKHESTCSLSVV"
     gene            complement(6380..6658)
                     /locus_tag="P5293_02300"
     CDS             complement(6380..6658)
                     /locus_tag="P5293_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48771.1"
                     /translation="MASLRKIKKDIEYLFTEVISDCWTYLYFNKGKNEDKIAEIINDA
                     IDLRNDLFKRINHYDRKNARKTFQEINSDLLKGVDELFVRVSKLNEAK"
     gene            complement(6671..7825)
                     /locus_tag="P5293_02305"
     CDS             complement(6671..7825)
                     /locus_tag="P5293_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRR48772.1"
                     /translation="MKEVINSNRRDFLKKAGVITAAAAVINPFELLARPGYQKKVTSK
                     KMELTFRPFDAQMRHVFTIANSSRTTTPIVLTEIAYDGYVGYGEAAMPPYLGESQASA
                     IEFLKRVDLSQFKSPFEMQDILTYVDSITTNNTAAKASVDIALHDLVGKIMGQPWWKI
                     WGFNAATTPNTSFTVGLDTEEVVKEKTREASPYKVIKVKLGRDEATDKMMINTIRSVT
                     NTTICVDANQGWKDKYYALDMINWLNERNVSMIEQPMSKYMLEETAWLSERSPLPIIA
                     DEACQRLTDVPKLKGVYHGINIKLMKCTGMREAREMVSLAKALHMKLMIGCMTETSCA
                     VSAAAQLAPEMEWADLDGNLLIANDVFKGMTVVDGKVTLNEKPGIGIEKI"
     gene            complement(7827..9053)
                     /locus_tag="P5293_02310"
     CDS             complement(7827..9053)
                     /locus_tag="P5293_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRR48773.1"
                     /translation="MKKFIFLVLAILLTPVCKGDEKSEKTQKLFDSISKEIKAQYAPD
                     RRVKTYEPFLFFPKDGSVPVLKGSTTEAGAKEALLKALAANKIKIVDSMVVLPDPALK
                     DKIYGITALSVINFRYGPDYAEESATQTIMGTPLRILEKRGSWTRAVTPEGYIAWVTS
                     GSIMPMNEKEYMTWSSSPRLIITAYYTLFRDAPSDTSPVVSDGVMGCIVRVDGESGDY
                     YKVILPNGKKCFVQKTDATDFENWVDSRNPTPENIISTAKQFIGFPYMWGGTSIKAMD
                     CSGFAKTSYYLNGVILERDASQQALYGDSISTANNFEALKAGDLNFFGSKATPDRRER
                     ISHVGIYLGGGEFIHSATSVRINSLIPSASNYYEGSNRLVRVRRITPQIDKIKGDGIL
                     SIKNHPWYFPDKTKTN"
     gene            9113..9559
                     /locus_tag="P5293_02315"
     CDS             9113..9559
                     /locus_tag="P5293_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HRR48774.1"
                     /translation="MIIDSLRGFERYLNLHSKFEKVFSYIIKNDVHKLEPGEYSVVGK
                     DIYFTIWEGEGKGMVIPKLEVHDSYIDIHILLEGNETIGFRDRSRCNGDGITYDPEKD
                     IAFLEEMPENFINLAPDNLAIIFPYDAHAPLIGEGKIKKAVFKVKM"
     gene            9559..10491
                     /locus_tag="P5293_02320"
     CDS             9559..10491
                     /locus_tag="P5293_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRR48775.1"
                     /translation="MGQRYNTYVGYFKKRYGERLQKIVIDAGFSCPNRDGTLGTGGCT
                     YCNNAAFHPSYSSPDKSVISQINEGIEFHRKRYRTANHYLAYFQPFSNTYAPLKELKK
                     VYEEALSHPLVSGIVIGTRPDCIDDEKLDYFQELSKRKIVVLEYGIESVHDKTLKRIN
                     RCHTYAQSVDAITKSAARGLTLGAHFIFGLPGESKEDMMNMAFKINNLPLHSVKFHQL
                     QIIRGTAMEKEFKEHPGDFVNFTIDEYIEFFVNFLELLKPDIYIERFAGEVPPRFVEM
                     TPWGSIRNAELIRMLEKRLEERNTRQSAKFYPQL"
     gene            10738..11589
                     /locus_tag="P5293_02325"
     CDS             10738..11589
                     /locus_tag="P5293_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR48776.1"
                     /translation="MKKYLFLTILLPVLLSCKYINFSRDKIVAQVGKNTLLESDIREL
                     IPEGTAPEDSIRMVEQYINNWAITHLLLKKAEEELSKSEKDVEQELEDYKNSLLVYRY
                     ENLYVESHLDTTITEDECREYYEKHSSNFTANNTIVKARVIKISHNSPNLASIKNMYK
                     ANGIEEIDELERLCYNAADKYDNFDNNWVDISAVAALVPYDLQSCEKAANSSLSIETS
                     DSLYNYFVYFIDKISPGQIPPFDYYKPLIKETILIKRKQELINELERNLLKEALENNI
                     LKTNINK"
     gene            11586..12971
                     /locus_tag="P5293_02330"
     CDS             11586..12971
                     /locus_tag="P5293_02330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR48777.1"
                     /translation="MITNIKEWSRLLIFTAAIFLGYNSSASAQKYGGGLIDKIVAQIG
                     NEMIQLSTIEAEVQMMLFQGVPSDKNLRCEVLERLMEQKLFLAQARLDSLTPNMEMVE
                     QNLNQRMQEVMTRLGGEKATEEYFKKPLYKIKEEWRETLTELSMVNNMQAEVAKKAPE
                     LTPSDIEKYYKRLPKDSLPIISTQYQYSQIVLYPEKEAAIMAVKERLLEFRERIMKGE
                     KFSTLATMYSQDFASASKGGELGMASKQLYWSQFSDAAMALKPGQVSQIVETPDGFHI
                     IQLIKKEGDMFNARHILLKPMYTNSDKDKAFKTLDSLRNMILLDSITFEQAARRFSQD
                     PKSYVNGGLVADENTGSVYFLVDRLKPEDYAVLKTLQVGEISAPFESRDNEGREGNIV
                     YKIVRLDKVIPSHVATFKDDFYILQQMAENEAINKALDEFIKEKQETTYIKIDPMFEN
                     CSFKRDGWIKK"
     gene            12968..14812
                     /locus_tag="P5293_02335"
     CDS             12968..14812
                     /locus_tag="P5293_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRR48778.1"
                     /translation="MMYRSLITILLSILPSFLFSQQQQVQRPEKSLIRLIDAKSAVLT
                     TSMGREYREIKGPAQFLHDNTLILCDSAIWDTQANIVDAIGHVKIIQKNTTLYSDNIK
                     YLADQSLAQVRGNLVELIDNEGGRLRTNFIDYYTKDSIGMFYNGGCLMNEDSSFIESS
                     NGYYYAKEDRFLFQMKVEMQSDTVLMKTDSLAYLAKENRAVFLTKTLAWQGDGFLMAN
                     GGWYDRKNEYIHFSNDVYIKTKNNEIWAREIDFDRKTGNAKLEYDVQVLDTAQSAIIL
                     ADRVHYTSKPESIVLEKEPCIALFTVENEVRDTLFFSADTIIYQTRLHKDVDSSVIAV
                     SKQRRDLSKRDPITEIYSSLKAKTSLLNKKNSDTTAVKNYDSTQVAKKDTVAVDTVAV
                     NIDTVKVKFLTAFKNVKFYRSNLQGVCDSLQFNSLDSLIRLYKEPILWSENKQLSADS
                     IQVILSGRKLKKAELNSSAFVAMQEDSSHFDQVKSADIIIFFDDGKLSRFDALGSASV
                     LFFFEEDSVLTTMNEKECKALSATMLNNKIHRVRYFENINSNAYPLFNLPDNKQKLKG
                     FKWADEKRPKNRYDVCNRTIKSSQASTLEMAEKPLFTQFGIYFSKTKK"
     gene            complement(14822..16102)
                     /gene="glyA"
                     /locus_tag="P5293_02340"
     CDS             complement(14822..16102)
                     /gene="glyA"
                     /locus_tag="P5293_02340"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRR48779.1"
                     /translation="MKQDLQISELVKKEENRQLHGIELIASENFVSNQVLAALGSVLT
                     NKYAEGYPGARYYGGCEVVDQVEQLAIDRICQLFNAEYANVQPHSGAQANMAVFMACL
                     KPGDTFMGLDLSHGGHLTHGSPVNMSGIWYKAVSYQLNKETGLVDYDEMERKALEYKP
                     KLIVGGASAYSREWDWARMRAIADKIGAILMVDMAHPAGLIAAGLLDNPVKYAHIVTS
                     TTHKTLRGPRGGIILIGKDFPNPYGILTPKGEVKMMSAVINSSVFPGIQGGPLEHCIA
                     AKAVSFFEALQPEFKTYQLQVKNNAAVMASSFIERGYKVVSGGTDNHLMLIDLRSKFP
                     DLTGKVAEKLLVSADITVNKNMVPFDSRSPFQTSGIRVGTPAITSRGLVEKDMPYIVE
                     LIDRVLSNPDNPAVISEVKELARKKMHGLPLNIW"
     gene            complement(16194..>17718)
                     /locus_tag="P5293_02345"
     CDS             complement(16194..>17718)
                     /locus_tag="P5293_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRR48780.1"
                     /translation="VLLSLFAIALIIPFIHISFAKDVPYSGLALNIDQLIAMAASEES
                     VAVAEKSSVDVWILGIFILYIAGVLFFLARMALSMVRIIMMVRSSDTQRQVVEGVSLI
                     IHKKEMAPFSWMRYIVISEKDYLESSREIITHELAHIEGRHSLDLILAEVITIMHWFN
                     PAAYLLKQELRNIHEYQADEAVIDKGIDAKQYQLLLIKKAVGDRLYTMANSFNHSKLK
                     NRITMISKKKSNRMAAVKALFVLPLSALAVVAFASEKMTSEMEVISNAKITEIFAPDT
                     IKNNVKKEANVKVKSEGQVKGEKTYTITVNDENQGNTSTEKVVKYMVRDTINVTIAGK
                     DTAKAGFRTGYIRINNADTKDTIRTFVTFMDESKTDVIPLCIIDGKEVDIKVMKALDP
                     GAIHSISVLKDKAAIDIYGDKGKNGVLVITLKSVSVDRSYDDKNITTIRIRGAKENND
                     VLYVIDGKVMDSKDAMEIEPDQIKSMSVLKGDVAKAKYGDKVKSGVIEITLKEKTQR"
BASE COUNT         5482 a         3731 c         3136 g         5369 t
ORIGIN      
        1 ttaacgtcag tattgatacc accaaataat tttggattaa catcaccaag aatcacctca
       61 cttgccttgg aatatgaaaa tgtagccggg cgtccattga cagtcaaatc aggagttgct
      121 tcgttattaa gataccaaac attctttccg gtctcgtcat ctacccctgc ccactcaagt
      181 ccatacagag caagagttga gcctccctca acgtatctga attttgctct tgcatctcct
      241 cctgtagggt cataccatgt aattggctgt cctccatata gtttattcac tttagactta
      301 attgaagagg ctgtaatccc tgcactccat ctaagatcct tttttctaat aatatctcca
      361 ctgatctcaa tttcaatacc atggttgttg atttcgccaa tgttcttaag tgtggagcca
      421 aaacctgtaa caagtgaaat tggaacatcc tggagtaaat ctttagaagt tctgtcaaag
      481 tattcaatag ttccggagag cctttgatca aaaagaccaa attccaaagc aaaattataa
      541 gtgtagcttg tttcccaaga gagatcagga tcagctatag tactaactcc gccgccaggg
      601 ttctgcatat aattattggt ataggaggtt aatgaacgcc atccgtaatt agaagttgga
      661 agggtaccat taacaccata agaggctctt aatcggagat tacttatata atcaagattc
      721 tccataaact tctcactgtt tatcttccat gatccagcaa ctgaccagaa atcaccccaa
      781 cgattatttt ctccaagtct tgaagaacca tctctacggt aagatgctga gaaataatat
      841 ttgcttagat agttgtactc aatacgagag agtgcagata gcattgtatt gccccaatta
      901 taccctgctg catcaagttt ccctgctgta gagacagtat gaagagtgct tgtaggcaaa
      961 tttgttcctg ttgacctctg gaaataagtc tgattatctt ctgcttccca tccaatcaat
     1021 acacctaagg tatgcttgtc tgcgaaaact ttattgtaac tcaatgttga tgatgatacg
     1081 agtttagatg tatttgtact catttcgttt acagaccctg aggttgaaga gccattgaag
     1141 tgtaatgcgc tgtaatagat atgatccatt gtattagtat tgtcataaga aaatactgtc
     1201 ttaaatatca gctccggcaa aaacttaatt gtaagagttt cattagcact aatcttaagc
     1261 atctttgtcc agttctccca ctgttcattg tagtaaacag gattataggc ataactacca
     1321 tatctgtcag tccatggctg acctgtgaca acatttgttg gccagtaaag aggccacaac
     1381 aaattccttg attgcatgaa gtagtttgct ccagtggaac gagtatcatt aaatccactc
     1441 ttttcgcttc tcgcaatatt aatattggtg gtaaactcaa tgaacctgtt aatcttttga
     1501 ttcaaattaa cacgacctgt tacacgctcg taatcattga tagttatcct cccctgctct
     1561 ttggtgtaag aaagtgatga atagtagtta gatctgtcag atgccccact gactgacata
     1621 tcataggtct gataaacagc tgttctgaaa agaacatcat cccagttgta gaacgttcct
     1681 gctctgtcac cgccaactgt gagagagttg acagtattgt ctggagcagt aaaagtataa
     1741 ccgtgcttat taaacctctt gttcatctga gcaagggcat ttgcacttgc ttgctcagga
     1801 gttttgtttt gataataaat tcctgcattc cagaaatttt catagtacaa ctctagttgc
     1861 tgctcaggag tagctgtctc atagttatct gtagcccaat caggtgagat gcccactgag
     1921 gctttaaagt taattacagg cttcccctct ttcccctttt tggtcgtaac aacgataaca
     1981 ccatttgcag cacgtgaacc gtaaagtgct gatgccgctg catctttcaa cactgtaatt
     2041 gactcgatgt cactcggatt aagggtgctc attacattgt tcgaagtgta gagatagtct
     2101 cccatctgtc cggagctccc tgaaatagca gggacaccat caattacata aagcggctcg
     2161 tttgatgcgt tcatcgaacc tataccacga atacgtattg atgatacaga acctgcctgc
     2221 ccagagttcg ttgtgatttg catgcctgca atcttgccac tcaaagcatt ttcaaatgaa
     2281 agagtcggta catcctttat agcgtctgct ttaacgacac tggcggcacc ggtaaatgta
     2341 ctcttcctgg cagtaccata ggcgacaacc atcacctcat caagattaag tacatcagag
     2401 acaagctcaa tatttacctc actccttccc tgcactggta cttcctttgt ggcgtacccg
     2461 atgtaggtca cgacgaggat ggcatctcgt gaaacattct ctatcgagaa tctcccatcg
     2521 agatctgttg tggcaccgat attcttcatc ccttttacct gaatattggc ataaggaata
     2581 ggagaaccgt catctgaaga tgtaactctt ccggtaacct taatctgtgc catagctaca
     2641 ttacaaagca gcaaaacaac gacaaacaag atctttttct tcatagataa gatagtttaa
     2701 ataatttata gatgttcgac ttcgaattta tcattaattt ctattctttg caaacaaatc
     2761 tttagaaact caaagtctaa agccgtaaga acaaggctta cacaaattac aattttatat
     2821 aaaaaatggc cgtttctatg cttgaaacga ccataactaa aaaataagtt ataaaatgtt
     2881 agtttttctt atcactttta aacggttttt tatcaccctc ttttcttggt ttcctcttag
     2941 gttcgatgta tccttccggc ttaggaagca atgcttttcg agagagtctc atttttcctg
     3001 tttttggatc tgtctcaagt aatttcacat ccacttcatc tccaatcttt aattcatcct
     3061 caaccttatc aatttttttc caatctatct cagaaatatg caataagccc tccttccccg
     3121 gcaaaatctg aacaaaagcg ccaaaatcaa gtattgaaac aaccttacct ttatatactt
     3181 ctccaacctg tggaacagct gttattccct taatccaacc aagcgccttc tccatactct
     3241 ccttatcttc tccgaaaatt tcaacaactc ctgttccgtt ctcctctgaa atagtaatag
     3301 tagtaccggt aactttttga atctcttgaa taacttttcc accagtgcca ataacagcac
     3361 caataaattc ttgtggaata ataacctgga tgattcgtgg aacatgtggc ttgtagtcgg
     3421 cacgcggttc acttatgcat ttcatcatct ctcccataat atgtagacgg ccttgacgag
     3481 cctgttcaag agctttttca agaatatcgt atgagagtcc gtcaaccttg atatccattt
     3541 gagtagctgt gattccgtcc tttgtacctg ccactttgaa gtccatgtca ccaagatggt
     3601 cctcgtcacc taaaatatca gacaaaattg catatttgcc atcctttgtg attaggccca
     3661 tagctatacc ggaaacaggt tttttgattt taacacctgc atccataaga gcaagcgtac
     3721 cagcacatac agtagccatt gaagatgaac cgttagactc aagaatgtcg gacacaactc
     3781 ttacagcata aggattttcc tcacctgtag gaacaacaaa tttaagagct ctcaatgcca
     3841 gattgccatg acctatttct cttctgcctg ttcccctgta tggtttagcc tcacctgttg
     3901 agaagggagg gaaattgtaa tgaagtacaa attgttctat tccctcaacc aatacttcat
     3961 caatctcttt catatcaagc ttagttccca atgtaacagt tgtaagagac tgcgtttcac
     4021 ctcttgtaaa aatggcagat ccatgagttg ccggaagata atctacttca caccaaatag
     4081 gccttatctg ggtggtagtt ctgccatcaa gacgaatacc ttcatcaagt attaaattcc
     4141 tcatggcctc cttctcaact gcatgataat atctatctat cataatcttc ttcgccgctg
     4201 catcctcttc tgataaagtt gcaacaaatt ctgccttaat gttatccaat gcctctgtac
     4261 gcgcctgctt tcccaaccct gccttagcta cggcataaca tctgttataa caaaactcct
     4321 taattgccgc acgaagatct tcatcctggg tttcatgaca gtatgccctc ttctgagtct
     4381 tgccaacctc ttccatgagt tccatttgag ccttacaatg aatcttaatc tcctcatgag
     4441 caaatttgat cgcctcgagc atgtcttttt cactaacctc cttcatctct ccttcaacca
     4501 taaggatatt gtcgatagtt gctccgacca taatatcaat atctgctttt tcaagctcag
     4561 aaaaagttgg attaattaca aactgacctc ctatcctcgc cactctaact tcggatatcg
     4621 gaccattaaa aggtatgtca cttacagcaa tagcggcaga agctgcaaga cctgcaagtg
     4681 catcaggcgt gatctcttta tctgcagaaa tcaaattaac atttacatac acctccgcat
     4741 gataatcatc cggaaaaagt ggtcttaatg ctctgtcaat aaggcgtgat accaaaattt
     4801 cggaattatt tgcacgtccc tctcttttga gaaatccacc tggatatctg cctgcagaag
     4861 catatttttc cttatattca actgaaagtg gcatgaaatc tacatcctct ttagcttcct
     4921 tagctgccac aactgttgcc agtaacattg ttttgccttg ttttacaaca acagcaccat
     4981 ctgcctgctt tgcaagctta cctgtttcaa tttcaatttc tctaccatct ctcagccgga
     5041 tggttttttt aactacattc atatattcat ctgtgcttcc cgctctccct atgagagttt
     5101 attattaatt atttatcagc tatacattca ccccgggtcg cattatttag gcaacatgta
     5161 taaaaatctt gcaaatgtaa tcagatatga caaaatgaca aacaaaaaaa aaggtgcctc
     5221 aaggcacctt ttttctattt tctaaggttc aaggccttta ctatatttct gtatctctct
     5281 atgtctgtct ccttgaggta atcaagcaga cgacgtctct taccaaccag ttttaaaaga
     5341 gaacgctgag taacgtaatc tttcttgtta tttttcaaat gatttgtaag atgagcgata
     5401 cggtaagaaa atagagctac ctggctctca ggagaaccag tatccgcatt agactttccg
     5461 tattgtccga aaatctcttg cttcttttcc ggtgtcaaat aattcataaa attaaagcaa
     5521 ttgatattta tttcccatta tttgggttgg caaagatata caattatttt cagctcacaa
     5581 tttttttgaa tacttttacc aattccaact tgctatttaa acctaaacta tcaatattgt
     5641 gataaaaaaa tatatccatc cacgtttcgg agagataatt attacgaaaa aaaaaggtca
     5701 gcgcaatatc agacttactg ttagtctcac taaaggagta agagtatcta tcccttatct
     5761 cacaagtttc tcctcagcag aaaaatttat taaagccaaa gaagagtgga tactatccac
     5821 aattcaaaaa aacagagata aacaacttgc caggattatc ccaataggtg aagggcaaaa
     5881 acttcaactt gtaaacagca aggtaatata tcaacaaatg tcctgcaggg aaaaatcaat
     5941 gccaggtaag atattaatcc gctacctaaa tgaagttaca gaagttaatc ttccacaaag
     6001 ctgcagcaaa gaggcagtac taaatgccac aatggttgtt atcagacgaa aggcagccga
     6061 ctatcttcca caaagatgta aattccttgc ttctgaaaac ggattcagct acaataaact
     6121 atttcttaaa aataataaaa ctaattgggg cagctgttca aacaaaggga atataaatct
     6181 caacattcaa ttaatcaggc taaaggccga actcattgac tttgtaattc ttcatgaact
     6241 ttgtcatctc aagtaccata accatggaac ggccttccat aacctgttaa acaaactttg
     6301 tcaaggcaga gaaaaagagc tggcacttca acttaagcat gaaagcacct gctctttgtc
     6361 agttgtctga aaaagccgat tatttagcct cgttaagttt actaacccga acaaatagtt
     6421 cgtcaactcc ttttaaaaga tctgaattta tctcttgaaa agtcttcctt gcatttttcc
     6481 tgtcatagtg atttatcctc ttaaagagat catttctcaa atcaattgca tcattaatta
     6541 tttctgctat tttatcttca tttttaccct tattgaaata caaatacgtc cagcagtcag
     6601 aaataacttc agtgaaaagg tactctatat ctttcttgat ttttcttaaa cttgccataa
     6661 tccccaaaaa ttatattttt tcaatcccta taccgggctt ttcatttaaa gtaacttttc
     6721 catccacaac cgtcatgccc ttaaaaacat cattggcaat tagaagatta ccatcaaggt
     6781 cagcccactc catttcaggt gctaactggg cagcagcgga aacggcacag gatgtttcag
     6841 tcatacaccc aatcatcagt ttcatgtgca atgctttggc caaagagacc atctcccttg
     6901 cttctctcat cccggtacat ttcatcaatt taatattgat cccgtggtat actcccttca
     6961 actttgggac atctgtcagc ctttgacatg cctcatccgc aataataggc agtggacttc
     7021 tttctgaaag ccaggcagtc tcttcaagca tgtatttgga cattggttgc tcaatcatgc
     7081 ttacattcct ctcgttaagc caattaatca tatcaagagc ataatattta tctttccagc
     7141 cttgatttgc atcaacacaa atagttgtat ttgtaacaga gcggattgtg ttgatcatca
     7201 tcttgtctgt cgcttcgtcc cttccaagct tgacctttat taccttataa ggagaggctt
     7261 ctcttgtttt ctcctttact acctcctccg tatcaagccc tacagtgaaa gaggtatttg
     7321 gagttgtggc agcattgaaa ccccatattt tccaccaagg ctgccccatg attttgccga
     7381 caagatcatg aagggcaata tctacagaag cttttgcggc cgtattgttg gtggttatgg
     7441 aatcaacata tgttaaaata tcctgcattt caaaaggaga cttaaactga gataagtcaa
     7501 cccttttcaa aaattctata gcagaagctt ggctctcacc aagatatggc ggcatagctg
     7561 cctcaccata accaacataa ccatcatatg caatttcagt aaggactata ggagtagttg
     7621 tacgggaact attggcgata gtaaaaacgt gtctcatctg agcatcaaat ggcctgaaag
     7681 tcaattccat ttttttactg gtcactttct tctgataccc gggacgggcc aaaagctcaa
     7741 agggatttat tacagcagcg gcagcagtaa taactcccgc tttttttaaa aaatctcttc
     7801 tgttactatt aattacctct ttcattttag tttgttttag ttttatcagg gaaataccag
     7861 ggatgatttt ttattgaaag aataccgtca cccttaatct tgtctatctg aggggttatc
     7921 cgtcttactc ttaccagtct attggagcct tcatagtaat tggaggcact gggaataagc
     7981 gaattgatcc tcacagaagt agctgaatga ataaattcac caccaccgag atatatgccg
     8041 acatgagaaa tacgctccct tctgtctggc gttgccttgg acccaaagaa attaagatcg
     8101 cctgctttca atgcttcaaa attgttagca gtagaaatac tgtcaccata taaggcctgc
     8161 tgagaagcat ctctttcaag aattaccccg tttaaataat aagatgtctt ggcaaagcct
     8221 gaacaatcca tcgctttgat agaggtacct ccccacatgt atgggaagcc gataaactgt
     8281 tttgcagtag atataatgtt ttccggtgta ggattccggc tatcaaccca attttcaaaa
     8341 tcagtcgcat cggttttctg aacgaaacat tttttgccat taggtaatat aaccttgtaa
     8401 taatcacctg attcaccatc tactctgact atacaaccca taacaccatc tgaaactaca
     8461 ggtgacgtgt ctgaaggagc atccctaaac aaggtataat aggctgtaat aatcaacctc
     8521 ggtgatgagc tccaagtcat atactccttt tcattcattg gcatgatgct tccggaggtc
     8581 acccatgcta tataaccctc aggggtaaca gctcttgtcc agctgcctct tttttcgagt
     8641 atcctcagtg gtgtacccat aatagtctgt gttgcagatt cttctgcata atcaggacca
     8701 tatctgaaat taataacaga cagtgcagta attccataaa ttttatcctt aagggcaggg
     8761 tcaggaagaa caaccatact gtctacaatc ttaatcttgt tggcagcaag tgcctttaac
     8821 aatgcttctt tagctcctgc ttcagtagtt gaacctttta aaacaggcac acttccatct
     8881 tttgggaaaa aaagaaaggg ttcgtaagtt tttactcttc tgtccggagc atactgtgct
     8941 ttaatttcct tagaaataga gtcaaacagt ttttgtgtct tttccgactt ttcgtctccc
     9001 ttgcataccg gcgtcagtaa aatagccaaa accagaaata taaacttttt catctttgag
     9061 ggttaattat tagacaaaca aagataatta tatttgcaac aaaaatctta ttatgattat
     9121 tgattcttta aggggttttg aaagatatct caacctgcat tcaaagtttg agaaagtgtt
     9181 tagttatata ataaaaaatg atgttcataa acttgaaccc ggagaataca gtgttgtagg
     9241 aaaggatatt tattttacta tctgggaagg agaaggcaaa gggatggtaa ttccaaaact
     9301 cgaagttcac gattcataca ttgacattca catactactc gaaggcaatg aaaccattgg
     9361 cttcagggac cgaagtagat gcaatggaga cggtataact tatgatcctg aaaaagatat
     9421 tgcatttctc gaagagatgc ctgaaaattt catcaacctc gctccggata atttagccat
     9481 aatttttcct tatgatgcac atgcaccact tattggagaa gggaaaataa aaaaagctgt
     9541 ctttaaagtc aaaatgtagt gggacaacgt tataatacat atgtcggcta cttcaaaaag
     9601 aggtatggag agagattaca aaaaatagtg atagatgcag gtttttcttg tcccaacaga
     9661 gatggaactc ttggcacagg tggttgtact tattgtaaca atgctgcttt tcatccttct
     9721 tacagttctc cggacaagag tgttatttct caaataaatg aaggaataga atttcacaga
     9781 aaaagatatc gtactgccaa tcactatctt gcatattttc agcccttttc aaacacatat
     9841 gcccctttaa aagagttgaa aaaagtttac gaagaggctc tctcccaccc cttggtttca
     9901 ggaattgtaa taggaacccg ccctgattgt attgatgatg agaaattgga ttattttcag
     9961 gaactttcta aaagaaaaat agttgttctt gagtacggta ttgaatcagt tcacgacaaa
    10021 actctcaaaa gaatcaacag atgccacact tacgcccaat cagtagatgc aattacaaaa
    10081 agtgcagcaa gaggtcttac tttgggagct catttcattt ttggactgcc aggagaaagc
    10141 aaagaagata tgatgaacat ggcttttaaa attaataatc ttcccttgca ttctgtaaag
    10201 tttcatcagt tacagattat aagaggaact gctatggaga aagaattcaa agaacatccc
    10261 ggggatttcg tcaatttcac tattgatgag tacattgaat ttttcgtaaa ttttctggag
    10321 ctgcttaagc ctgacatata catagaacga ttcgcaggag aagtaccacc aagatttgta
    10381 gagatgactc catgggggtc catacgtaac gccgaactca taagaatgct tgaaaagaga
    10441 ctcgaagaac gtaatacaag acaatcagct aaattttatc cccaacttta atcctcaagc
    10501 attgcactta tcgatatcct ctctccagta gcctgcatga ataactggca ttataataag
    10561 taaaattgtt ataattgtta tgtttttcat ggtagtggat ttgactttta aggtaaccat
    10621 tttttatata cctttgcaat agtccgataa gagtatgatt ctgcaaactg tggcaggatc
    10681 tttgttcatt ggaatcaaga cttctgctga taagatataa ttaatattaa ttatacaatg
    10741 aagaaatatc tgtttcttac tattttattg cctgttcttc tatcttgtaa atacattaac
    10801 ttttccagag acaagatagt cgctcaagtt gggaaaaata ctcttctaga atcggacatc
    10861 cgcgaattga taccagaagg tacagctcct gaagacagta taagaatggt tgaacagtac
    10921 attaacaatt gggctattac acatctgctg cttaagaaag ctgaagaaga actgtcaaaa
    10981 agcgaaaagg acgttgagca ggaattggaa gattacaaaa actccctcct ggtatacaga
    11041 tacgaaaatc tttacgtcga atcacatctc gacactacaa ttacagagga tgaatgcagg
    11101 gaatactatg aaaaacactc ttcaaacttt acggctaata acacgattgt caaggcgagg
    11161 gttatcaaaa tatcccacaa ctctccaaat cttgcatcca taaaaaacat gtacaaggct
    11221 aatggaatag aagaaattga tgaactggaa aggttatgtt acaatgctgc cgataaatat
    11281 gacaattttg acaacaattg ggtagacatt tcggctgttg cagctcttgt cccttatgat
    11341 cttcaatctt gcgagaaagc cgctaacagt agtttgtcaa ttgaaacgag tgatagtctc
    11401 tacaattatt tcgtctactt tatagacaaa atatcgcccg ggcaaattcc tccatttgac
    11461 tattacaaac ctttaatcaa agagaccata ctcattaaaa gaaagcaaga gctaatcaac
    11521 gaacttgagc gaaatttgtt gaaagaagct ttagaaaata atatattgaa aacaaatata
    11581 aataaatgat aactaatatt aaggagtggt ccagactgct catattcact gctgccattt
    11641 ttcttggata caattcatct gcctctgcgc aaaaatatgg tggagggttg atagacaaga
    11701 tagttgctca aataggcaat gaaatgattc aactctcaac cattgaggct gaggtacaaa
    11761 tgatgctttt ccagggagta ccttcagata aaaatcttcg ttgcgaagta cttgaaagat
    11821 taatggaaca aaagctcttt cttgctcagg cgagactgga cagtttaact cccaatatgg
    11881 agatggttga acaaaatctt aaccaaagga tgcaggaagt aatgactaga ctgggaggag
    11941 aaaaggccac agaagaatac ttcaagaaac cactgtacaa aattaaggag gagtggcgtg
    12001 aaactctcac cgaactctcc atggtaaata atatgcaggc agaagttgct aaaaaagcac
    12061 ctgaacttac accttctgat attgaaaaat attataaaag actgccaaag gacagccttc
    12121 caatcatttc tactcaatat caatacagcc agatagtgct ttacccagaa aaagaggccg
    12181 ccattatggc tgtaaaagaa aggttactag agtttagaga gagaatcatg aagggagaaa
    12241 aattcagtac tcttgcaaca atgtattctc aggattttgc cagcgcttcc aagggtggag
    12301 agcttggaat ggcgtcaaaa cagttgtact ggtctcaatt ctcagacgca gccatggcac
    12361 ttaaacctgg tcaagtttct cagatagtcg aaacccctga tggctttcat attatacaat
    12421 taatcaaaaa agaaggcgat atgttcaatg caaggcatat tcttcttaaa cccatgtaca
    12481 ccaattcaga caaggataag gctttcaaaa ccctcgacag tctgagaaat atgatacttc
    12541 ttgacagcat aacttttgaa caggctgcaa ggcgtttttc tcaagatcct aaaagctatg
    12601 ttaatggagg acttgtggct gatgaaaata caggttcagt gtattttcta gtagacagac
    12661 ttaaaccaga agactatgct gtactaaaaa cactccaagt cggtgaaatt tccgctccat
    12721 ttgaatccag agacaatgag ggcagagagg gtaatattgt ttataaaatt gtacgtctcg
    12781 ataaagtaat cccctcgcat gtagccacat ttaaggatga tttttacatt ctgcaacaaa
    12841 tggcagaaaa cgaagctata aacaaggctc ttgatgaatt tatcaaagaa aaacaagaaa
    12901 ctacatatat aaagattgac ccgatgtttg aaaactgttc attcaaaaga gatggctgga
    12961 tcaaaaaatg atgtacagga gcttaataac aattctacta tctatactgc cctcttttct
    13021 tttctcccaa cagcaacagg tccagagacc tgaaaagagt ctgatcagat tgattgatgc
    13081 taaatctgct gttctgacta catcgatggg aagagaatat cgagagatta aagggcctgc
    13141 tcaatttttg cacgacaata ctttgattct gtgcgattca gccatatggg acacccaggc
    13201 caatattgtg gatgcaattg gtcatgtcaa aataattcaa aaaaacacaa cactctatag
    13261 tgacaacata aaatatcttg cagatcaatc tcttgctcag gtaagaggca acttggtaga
    13321 gctgatcgac aacgaaggtg gcagactccg tacaaacttc attgattact atacaaaaga
    13381 cagtattgga atgttttaca acggaggttg cctgatgaac gaggatagca gctttataga
    13441 gagttctaat ggatattatt atgccaaaga ggacaggttt ctgtttcaaa tgaaagtaga
    13501 gatgcaatca gataccgttt tgatgaaaac agattcactg gcttatcttg caaaggaaaa
    13561 cagagctgtt tttcttacta aaactctggc atggcaagga gatggatttt taatggcaaa
    13621 tggtgggtgg tatgacagaa aaaatgagta tatacatttt tctaatgatg tatatataaa
    13681 aactaaaaac aatgaaatat gggcaagaga gatagatttt gaccgtaaaa caggtaacgc
    13741 aaaactggaa tatgacgttc aggtactcga tacagctcaa tcagccatca tcctcgctga
    13801 cagagttcac tacacaagca agcctgaatc gatagtactg gagaaagaac catgcatagc
    13861 actttttaca gtggaaaatg aagtcagaga tacacttttt ttctcagccg atacaataat
    13921 atatcagacc agactgcata aagatgttga ttcctctgta attgctgttt cgaaacagag
    13981 gagagatcta agcaaaagag acccaattac tgaaatatac tcctccctaa aagccaaaac
    14041 atctctcttg aataaaaaaa attctgatac aactgcggtt aaaaactacg attcaaccca
    14101 ggttgccaaa aaagatacag tggcagtaga tactgttgct gtaaacatag acacagtaaa
    14161 agtaaagttt ctgacagctt ttaagaatgt gaaattttac cgtagcaatc tacagggagt
    14221 ttgtgattct ctccaattca attcccttga ttctttaata aggctctata aggagccaat
    14281 attatggagc gaaaacaaac agctttcagc agatagtatt caggttattc tttcaggcag
    14341 aaaacttaaa aaagcagagt taaattcttc agcatttgta gccatgcagg aggactcatc
    14401 tcacttcgac caagttaaga gtgcagatat tataatcttc tttgacgatg gaaaactcag
    14461 cagatttgac gctcttggca gtgcttcagt tctttttttc tttgaagaag acagcgtctt
    14521 aacaacaatg aacgaaaagg agtgtaaggc tcttagtgcc actatgttga ataataagat
    14581 acatagagtt cgctactttg aaaacataaa tagtaacgca tatccgctat tcaatctccc
    14641 ggacaacaag caaaaactca aaggattcaa atgggcagat gagaaaaggc ctaaaaaccg
    14701 ttatgatgtc tgcaatcgta ccatcaaaag ttctcaggcc tcaacattgg aaatggcgga
    14761 aaaaccgctc tttacacaat tcggaatcta cttttctaaa acaaaaaaat gattcttagt
    14821 gttaccaaat attaagaggt aatccatgca tctttttacg ggcaagttct tttacctctg
    14881 atatgacagc tggattatcc ggattggaaa gtaccctgtc aatgagttca acaatataag
    14941 gcatatcctt ctcgaccaag cctcttgaag taatggcagg agttccaact cttattcccg
    15001 atgtctggaa aggagatcga ctgtcaaaag gaaccatgtt cttattgaca gttatatccg
    15061 ccgatacaag caacttctct gcgactttac ctgtgagatc agggaactta cttcttaaat
    15121 ctataagcat gaggtgatta tctgtacctc cagatactac cttataacct ctctcaataa
    15181 aagaggacgc cataacagca gcattattct taacctgtag ctgatatgtc ttgaattccg
    15241 gctggagagc ctcaaagaat gagacagcct tcgcagctat acagtgctcc agtggtccgc
    15301 cctgtatgcc cgggaaaacg cttgaattaa taacagcaga catcattttg acttcacctt
    15361 ttggtgtaag aataccatat gggtttggaa aatcctttcc tatcaaaatt atgcctccac
    15421 gtggtcctct aagggtttta tgtgtagtag aagttactat atgcgcgtac tttacaggat
    15481 tgtcaagcag tccggcagca atcagaccag cagggtgtgc catgtccacc atcaggatag
    15541 ctcctatctt gtctgcaatg gctctcatcc tagcccagtc ccactctctt gaataagctg
    15601 aagcacctcc tacaataagt ttaggtttgt attcaagagc cttgcgctcc atctcatcat
    15661 aatcaaccag accagtttcc ttatttagtt gataagaaac cgctttatac caaatacctg
    15721 acatgttcac tggagatcca tgagtaaggt gccctccatg agacagatca agtcccatga
    15781 atgtgtctcc cggcttaagg caagccataa agactgccat attagcctga gcaccggagt
    15841 gtggctgaac attagcgtat tcggcattaa aaagttggca aatccggtca atggcaagtt
    15901 gctcaacctg atcaaccact tcgcaaccac catagtatct tgctccggga taaccttcgg
    15961 catatttatt tgtaagcaca gaccctaaag ctgcaagcac ttgattactc acaaaatttt
    16021 ctgatgcaat taattctata ccatggagct gtcggttttc ttccttctta actaattcgg
    16081 aaatctgaag atcttgtttc attatgttat atttaaacta caaattatta taaaacaact
    16141 ttcaaaggta ataattattt cctgttattc ttttttaaat ttgagtttta tccttatcgt
    16201 tgtgtcttct ctttgagagt aatctctata acaccactct taaccttgtc tccatatttt
    16261 gcctttgcta cgtctccctt gagaactgac atagacttaa tctggtcagg ttctatttcc
    16321 attgcatctt tggagtccat aaccttaccg tcaattacat aaaggacatc attattttct
    16381 ttagcacctc ttattctaat agttgttata tttttatcat cataagacct gtcaaccgac
    16441 acacttttaa gagttataac aagaaccccg ttctttcctt tgtctccgta aatatcaatg
    16501 gcagccttgt ctttcagtac agaaatagaa tgtatcgctc ccgggtcgag tgctttcata
    16561 acctttatat ccacctcttt tccgtcaata atgcagagtg gtatgacatc tgtcttactt
    16621 tcatccataa aagttacaaa ggtgcgtatc gtgtctttgg tatcagcatt gtttattctt
    16681 atatagcccg ttctgaaacc cgcttttgcg gtatcttttc cggcaatggt cacatttatc
    16741 gtatccctga ccatatattt tacaaccttt tcagttgagg tattgccctg gttttcatca
    16801 tttactgtta ttgtataagt cttttcacct ttcacttgcc cttcactttt cacttttaca
    16861 ttagcctcct ttttaacatt gtttttaata gtgtcaggtg cgaaaatttc agtaattttg
    16921 gcgtttgaaa tcacctccat ttcggaggtc attttctcgg atgcaaaggc aactactgcc
    16981 aatgcagaca acggcagcac aaacaaagcc ttgacagctg ccatacgatt tgattttttc
    17041 tttgagatca tggtaatcct gttttttagt ttactgtgat taaagctgtt ggccatagtg
    17101 tagagcctgt cgccaacagc ttttttaatt aataatagtt gatattgttt tgcatcgatg
    17161 cctttgtcaa taacggcctc atcggcctga tactcgtgta tgttcctgag ctcctgcttc
    17221 agaagataag ctgccgggtt gaaccaatgc atgatggtaa tcacttcggc aagaataagg
    17281 tcaagagagt gtcttccctc aatatgggca agttcatgtg taattatctc cctgctgctc
    17341 tccaggtaat ccttttctga aattacaata tatctcatcc acgagaatgg ggccatctct
    17401 tttttatgaa taatcaaact aactccttct acaacctgtc tctgtgtatc tgacgatctt
    17461 accatcatta taatcctgac catagaaaga gccatccttg ccagaaagaa gaggacacct
    17521 gctatgtaga gtatgaatat tccgagtatc cacacatcaa cactgctctt ttctgcaaca
    17581 gcaacacttt cctcactcgc agccattgca atcagctggt ctatgttcag agcaagcccc
    17641 gaatatggaa catccttggc aaaagagata tggataaatg gaattatcag agctatggca
    17701 aagagagaga gtaacact
//
LOCUS       DAOTQL010000019        17323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3201_length_17323_cov_5.614953, whole genome shotgun sequence.
ACCESSION   DAOTQL010000019 DAOTQL010000000
VERSION     DAOTQL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17323
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_17323_cov_5.614953"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..790
                     /locus_tag="P5293_02350"
     CDS             143..790
                     /locus_tag="P5293_02350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRR48781.1"
                     /translation="MELFFTNQITDSKASISGEEFHHCIKVLRYKKGDCVSFIDGKGG
                     LYSGVITDISSDCCNIRITDYTSDFAKRDYYLHMAVAPTKNFERYEWFMEKAAEIGID
                     ELTPIIGDHSVRRTFKKERGEKIFLSATKQSLKAKLPILNPMIQVEDFIIDQKDFDGI
                     KLIAHCNEGPKHNITSVLPKGCKFLILIGPEGDFSSEEVVLALENGFLYPLVIRD"
     gene            complement(854..1426)
                     /locus_tag="P5293_02355"
     CDS             complement(854..1426)
                     /locus_tag="P5293_02355"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRR48782.1"
                     /translation="MIDTKNAIEQAVKILKDGGVILYPTDTVWGLGCDATNAAAVERI
                     YKIKKREDNKSLIILVDGEDMLCRYIKEIPQVALQLIEIADQPLTIIYPGAIGLAKNA
                     IAEDNTVGIRIPHHNFCLQIIHRFNRPLISTSANISGEKAPANFINISEYIKSAADWV
                     ADPVFEADSTGKPSSIIKLGLGGEVQIIRK"
     gene            complement(1423..2508)
                     /locus_tag="P5293_02360"
     CDS             complement(1423..2508)
                     /locus_tag="P5293_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR48783.1"
                     /translation="MRKRKKGKKRSLWPISISFRILLFVAAAALGLSYISIYINPVDS
                     VIPLFFGLYFIPIVFLNIIILVIALIRSRSVAWVTFLMLIPSILFADLFVKFGKPNEG
                     EKGNALNICTYNVGLFAQESGISRLDQLNRVARYIGEERPSIVCFQEFYAKDTSVIRD
                     LFHQYPYIQWHFFRYSDGGMFGNLTISKFPIVSGDKITFKGSTNLCIFSDIDFFGKMI
                     RIYNTHLESHSISFTTLVKRMSNSQKVSEEIINVHERVASAFKKRGKQVDSIAVHMSK
                     SEYPAIICGDFNDTPMSYTYHILMKGMKDSFRESGRGFSATYSYLWPMLRIDYILYPE
                     KFWSMSHITQKVPYSDHYPVFSGIIIP"
     gene            complement(2508..3194)
                     /locus_tag="P5293_02365"
     CDS             complement(2508..3194)
                     /locus_tag="P5293_02365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRR48784.1"
                     /translation="MYYNTGRGTGFLGGMTPVIKNLVIINTLMLVITAFTGNFMYEKF
                     SLFYFGSPLFKPYQLITHMFMHGGFIHLFFNMYSLIFFGVILEQVWGSKKFFLYYMIT
                     GLGAAFLHSLVLYFDATSLLQAAEAGSIVAAEKYRILISTPTVGASGAIYGVLLAYGM
                     LFPNNVLQLLFPPIALKAKWFVLIFGAIELFFGISNTGGNIAHFAHLGGMIFGYFLIL
                     YWKKHNKMYF"
     gene            complement(3202..4938)
                     /gene="mutL"
                     /locus_tag="P5293_02370"
     CDS             complement(3202..4938)
                     /gene="mutL"
                     /locus_tag="P5293_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRR48785.1"
                     /translation="MIHILPGHITNLIAAGEVVQRPASVVKEMLENAVDAGADSIQVI
                     IKDSGRTLIQVTDNGSGMSREEAKIAFQRHATSKIETIEDLNHLNTYGFRGEALASVA
                     AVSEVTLRTRRESDEIGCEIKFAESSLISEKEISTPKGSNFIVRNLFYNVPARRKFLK
                     SDATEFRQIVQEFSRVAFTRPDLSLRLIHNDIEIFHLSPANLKKRILDIAGKEMGKEL
                     VHIHLESPIVKISGFTGKPEDSRKTPGNQYFFVNNRYFRSPYFQKAVVKAYENLIKEE
                     TIPSFFIFLEIDPERIDVNIHPSKTEVKFEDEFAIYDMLHAVVRESLGRNSLMPSIDF
                     DTEGAPEIPAVNYNYYAPPPKINFDPLFNPFEETLQYKRELMEPFVGETARLERPTVQ
                     VAGRYIITAIKSGLLIINIKRARERIFYEKYLDSLNNFSTISIRTLFPKSIELDENTY
                     NIILENAGILQSLGFDIRPKEDGSPRNVVEVMGLPENISDSTDDLLALIDQLVYDLTE
                     LGNSQLEDARQKYAATLALSAALCGKDSMNNQQAQLLIDTLFACREPSFTPSGKPCST
                     IITLEEIDKRFI"
     gene            complement(4938..5258)
                     /locus_tag="P5293_02375"
     CDS             complement(4938..5258)
                     /locus_tag="P5293_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48786.1"
                     /translation="MGFSFNFFRPTSHKVFHYEPQYYDPKKEAMEERYAQLNEEKGKS
                     KDGKYIPGKYIRTHMRNDVYKNKKDPGKVLTTRLIILISLVILFAIAFYLADGLGFIL
                     TSSK"
     gene            5337..5819
                     /gene="ispF"
                     /locus_tag="P5293_02380"
     CDS             5337..5819
                     /gene="ispF"
                     /locus_tag="P5293_02380"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRR48787.1"
                     /translation="MAVRIGNGFDVHRLNNGLPLWICGVRIEHSKGCIAHSDGDTPIH
                     ALCDALLGSLALGDIGKYFPDTSDEFKNIDSKILLHRVYEMVKTSGYSLVNADITILL
                     QRPKLANYISLMRETLAGVMEIDSSIISIKATTTEELGFIGKEEGIAAFASVIVEKLV
                     "
     gene            5882..5955
                     /locus_tag="P5293_02385"
     tRNA            5882..5955
                     /locus_tag="P5293_02385"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5916..5918,aa:Met,seq:cat)
     gene            6082..6984
                     /locus_tag="P5293_02390"
     CDS             6082..6984
                     /locus_tag="P5293_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRR48788.1"
                     /translation="MVNTSKLKSIVMPIALVVGIFLGLTIPKAVIVANAAIPYLIGLM
                     LLFTYSKLNFRELSIFPMPLVLILIQVGASIIAYLALYRWNPLFAQQAFICLLCPVAT
                     SAPVTTNILGGNTASIVSYTLLCYFVIALVFPLLLPLAGGVGEETFLSTSLVIAKQIM
                     PLIFLPLIVAVFVRKISYKFNSFLVNGQDIAFYLWGLTLTLVIGKATTYVAQQPSNLI
                     PMEIGLALIALVACLLQFAIGREVGLHYKDAIAATQGLGQKNIALAMWIGFTYLNPLV
                     SVGLAAYSIWQNIINSAQIYHKTK"
     gene            6989..7546
                     /locus_tag="P5293_02395"
     CDS             6989..7546
                     /locus_tag="P5293_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRR48789.1"
                     /translation="MKSRDYYFQLLKANVHNPKMIAHSLASEAVMRALAERLEEEKDL
                     WGIAGLLHDIDVELTAGDSCRHGLEGAKMLQNEDLPEEVLDAIIMHNEKASGKERTTK
                     FQHALAAGETITGFIFAIALVYPDKKISSVKVKSVVKRMKEKIFAASVKRENIRECEC
                     LGLQLEEFVELSLNALSPIESELGF"
     gene            7586..8989
                     /locus_tag="P5293_02400"
     CDS             7586..8989
                     /locus_tag="P5293_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRR48790.1"
                     /translation="MKRAVLFLTCLFTSCFAMLGQQIGTSELKEIKSGFIKDQKTTAI
                     QNFVSSDKDFKSKTLNRENQGKVDHFFKYRVNVKGITNQQNSGRCWMFTSMNVLRPVV
                     MDKYSLSEFDFSHNYSQFWDLFEKSNLFLENVIATAYKDFDDREVARYFSSPVDDGGV
                     WNLFYNVAEKYGVVPQSVMPETANSDNTGQMVGVLNERLRAGGYAIRELTKSSSKSKS
                     DSGQIEREMRNEKINVMKDVYRILAICLGEPPAQFTWRYKTKEGIIKSITTTPLDFYR
                     GIVPQDYNPKTFIMVMNDPTREYYKLYEIKNYRNTIEGINWCYLNLPNDEIKGSALAS
                     IKNNEPLYASCDVGKQLDREHGILDVNNYDYESLFGINMRMDKKARILTRQSGSTHAM
                     LLIACDTDDKDKPLKWEFENSWGASAGHNGYLTLTDDWFNEYMFRVVINKKYLSQKAI
                     DALGQKPVELPVWDYMF"
     gene            complement(9002..10465)
                     /locus_tag="P5293_02405"
     CDS             complement(9002..10465)
                     /locus_tag="P5293_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48791.1"
                     /translation="MIDIQASIHDNFSIEFKVGFHARRKVKINDFVMNTWIFIPDSLD
                     INHLTYPREHFYRDIRDNIRLITPIFLMRDIARGDMIPLKHLEASFHKLASYPSRSSN
                     ADYEYQIKMFAAIFKSALRDQIKYILSSAQPEDKGFLLDALIEDVRETLSKYRSLSTI
                     IKTPTVSKESYNYFEFGDEFMSNIVDQQFLTLLHKLEESDQTLADEYRGKIISLVEAE
                     NQYKKNQDYPVVSLDSPDNNRNLVFRRGVLKKFVESDLFLTAKKKKDGILVEQIYFSL
                     AAGISMIFATAVAFSFQRTYGNLTMPLFVALVVSYMLKDRIKELMRYYFAHKRKAKYF
                     DNKITVSIKDNIVGFSKEGFDFIPEKKVPKEILQIRNRSALLEADNRYTNEKIMLYRK
                     LLQIDRSKLDESSKYFVSGLNEIIRFNVQSFLSKMDNPETILFCINEKGELIQTLGAK
                     VYYLNFIIQLKYLDFESYKRYRVVFNREGIKEIEEML"
     gene            complement(10490..12085)
                     /locus_tag="P5293_02410"
     CDS             complement(10490..12085)
                     /locus_tag="P5293_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRR48792.1"
                     /translation="MEAKVQAFDPLDMNNYKIEKLPKLEKSTFEKWMAKMGFPLAIIA
                     FIVLYFIVDIPFLNNIDPGTLSGDAAKRFSELGAFGFSRANYAMLAVFVSSIILWITE
                     PVPNYLTSLIVILAIVLTGITTDKVAYAQLGHPVMWLNILSFILASMLVKTKVAKRFA
                     LWFVLKFGKNASMVIFSFIVINIVLSAFISATTAKAAILLPIFMVIAAIYGATGGNNR
                     NNFGRNLVLQNLFQINIGASGFLTGSGANLLSAALIAGSMGLTTFSYQDWFVAAFPLC
                     MILILIGWFVGMKIIFQLKDNEKKPSIEGGMNRLREELNSMGKMKFEEYKAIAIFVGV
                     LLLWATDKQHGINQTAVAFIGAVVALLPGIGVVKWNDVDIPWHLLLFSAGAYTLGAGL
                     EATGLPSTLVGQLFDNLGIGANTPFWVLYAILTAAMLFSALLFQSKTMRALIFVPIAI
                     GVAQKFGYPIMSLAFPVALLCEHVYVLPFNSKPAALLYTTNHYSWSDTFKFGMTMMII
                     SWVMILIWGETLLKWLGYTNGLL"
     gene            complement(12105..12572)
                     /locus_tag="P5293_02415"
     CDS             complement(12105..12572)
                     /locus_tag="P5293_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRR48793.1"
                     /translation="MGQEIERKFLVKGEFKHLASKSTRIIQGYLSSVPERTVRVRVKG
                     DKGFITVKGIGNNSGASRFEWEKEISVEEAQELLSICEPGIIDKTRYMIKVGPHTFEV
                     DEFYGENEGLTLAEVELSSENEAFEKPDWLGEEVTGDARYYNSMLMKNPYTKW"
     gene            complement(12577..13227)
                     /locus_tag="P5293_02420"
     CDS             complement(12577..13227)
                     /locus_tag="P5293_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48794.1"
                     /translation="MDKEKMVIGEISKPRFEFRTFGQDFSKAIARMARLSVPVPEKVW
                     ERFSDEIYIISRTNDINNTKIRDGKMDIKTLVQSIDGLEQWNPLMKGEFPISGDILRK
                     EVFPAFMVDIDITKLDKDFYTYEEFILMIKAHPDLQAVRVHKQRWGYMVNDTICEAGA
                     ILINGAKVMTLSSESTEIEDIKKTIKDIQIDGVENINYLQAIKRVIGMSDKKLANE"
     gene            complement(13253..14041)
                     /locus_tag="P5293_02425"
     CDS             complement(13253..14041)
                     /locus_tag="P5293_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRR48795.1"
                     /translation="MKKILIALLAAMFLIPGCVKDEQYEGPAVIEKITINPSSPMANQ
                     AITISCKITDLNGITEAVLKYKVNSGTQQSVTMTESEGRIFSAVIPGQADNASIVAVI
                     EATNLKGFKAVSEEQKIKVAPPILVYVNECDPNAKSMELYNDMTSTVDLGGWKLRKDG
                     STDASNNWTIPTGVTIPAKGYLVITQDVNGVTGFTFGMSPTKGFSYTLFDSNGATRDF
                     FDNLTTKITAGASPNTIGRVTDGAAQIVLFSVGSMGRSNATGVR"
     gene            complement(14073..15455)
                     /locus_tag="P5293_02430"
     CDS             complement(14073..15455)
                     /locus_tag="P5293_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRR48796.1"
                     /translation="MKKLIMITLATGLSLATWAQEKPQENVRYNQYGVKVNRNPLAVE
                     ERNGILVFESPSQDYRLWFDIRVQADGATFFGENKDFDKIGDGVSIRRARFAVKAQIS
                     KDWYGELDTDFANGSFELKDAILVYSGLRNFEFTAGNFKEGFSMECTTTSRYLALLER
                     PMVVQTFGPSRHIGLQAKYNWKWLLAEGGIHFQAVEGQETRTYVEDNNKDYGRSSGNS
                     YTGKFVIMPFYEKANYGLHIGAAASYRLPKSDVDPADYNGSRFSTRNATSINRKKYLD
                     TDVIDGTDHDFLYGFELAGFKNGFRFQSEYIGNDTYIKESSKLTNKDTKHFNGWYAQA
                     AYLLFGGKQQYNVQDGEFTQPTRGKSWGDIELVGRYDFLDLTSNGIFGGSGENYTFGI
                     NYYVNNSVKIMLNYMYSNNDRYANGKGKLYVGYDVDGNPTKDPTKVADAKGKAGVDYH
                     MLGIRFEIDF"
     gene            complement(15467..17119)
                     /locus_tag="P5293_02435"
     CDS             complement(15467..17119)
                     /locus_tag="P5293_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRR48797.1"
                     /translation="MIIEVLIIVIVTGLWLSLREFPIIIGHSFSSGILKLKYLPLVIA
                     FSILFSLPFTKGISNNFNDSFQITTPLIILLITLSSAIAMWIFSLFSKWNSYVYSLFG
                     AIMAYMVFMKNKFALTYFWQSVIAWVISPIISCIISIILFFAVKYLIKKIDLHLIVLN
                     RILSISLIVFLILTIFSFSINNGSIIIALTQPIDLMLNINLAGIFINSQYIVWAIFII
                     VVLALYFISIKEKASTLSIKLFDINTETSVIIMASVSLVMLLFSSNSVCSAIGLAATP
                     VSPVHVSLASIIGIGLIHKSIDLNKGEMIKSALSIVLLPAFSFLLTNLTLRTVIADSS
                     KQSLDITTFSIVTVLFLLVAIIVYFYISKNRDYEKTRLKRAEEINRLNEMQKVIVDME
                     IKVIQNENDNLHKRLEIKRKDLINNALWIGQQRNFLDEIYNEIKHLENIDEIEKIKEA
                     VRTLEKKIIDKKAFSHEINELYSQVELLHKDFSSILHERFPNLTEQEKRIATLLRLGF
                     STKELSSIMSITPKSVEVCRYRLRKKMNLKRDENLIEFIKSL"
BASE COUNT         5210 a         3143 c         3227 g         5743 t
ORIGIN      
        1 cgggattgtt gacaagaatc attcccgcaa tagtcatccc tctcaagacg tccagagcag
       61 tgtatctctg ggttattgaa gttgtcattt ttttagttct ttaatcacaa aaatagcttt
      121 ttataatata tttgcatcaa ttatggaatt gttttttaca aatcaaataa cagattctaa
      181 agcctcaatc agtggtgaag aatttcatca ctgtattaaa gtccttcgtt acaaaaaggg
      241 agactgtgtt tcatttatcg acggtaaagg tggactttac tcaggagtaa tcacagatat
      301 atcttcagac tgttgtaata taagaattac tgattataca tctgactttg caaaaagaga
      361 ctattatctt catatggcag ttgcgccaac aaaaaatttt gaaaggtatg agtggtttat
      421 ggaaaaagca gctgagatag ggattgatga attgacgcca attattggag atcattctgt
      481 aagaagaact tttaagaagg agcggggaga gaagattttt ttgtcggcaa caaaacaatc
      541 acttaaagcc aaattgccaa ttcttaatcc aatgattcaa gttgaagatt tcataataga
      601 ccagaaagat tttgacggga ttaagcttat tgctcattgc aacgaggggc cgaaacataa
      661 tattacttca gttttaccaa aaggttgcaa gtttttaata ctaattggac ctgaaggcga
      721 tttttcttct gaagaggttg tattggctct tgaaaacggg ttcctgtatc ccttggtaat
      781 tcgagattaa ggactgaaac cgcagcactt gctgttgtaa ttgctacata tcttcttact
      841 tcagagtttt aggctacttt cttattatct gcacctctcc tccaagtcca agtttaatga
      901 ttgaagatgg ttttcctgtc gagtcggctt caaaaacagg atctgctacc caatctgctg
      961 cagatttaat gtattctgaa atatttatga aattagcagg agccttttca ccggagatgt
     1021 tggcggatgt tgaaatgaga ggtctgttga acctgtgaat gatctgaaga cagaaattgt
     1081 gatgggggat tctaatccca actgtattat cttctgctat tgcatttttc gccagtccaa
     1141 tggctccagg atagataatt gtcagtggct ggtcggcaat ttcaataagc tgcagtgcaa
     1201 cttgaggtat ctctttaata tatctgcata acatatcttc tccatcaaca aggataataa
     1261 gacttttatt gtcttctctt tttttgattt tgtagattct ttctacagcc gcagcatttg
     1321 tagcatcaca gcccagaccc catactgtgt cagttggata gaggattacc ccaccatctt
     1381 taagtatttt tacagcttgt tcaattgcat ttttagtatc tatcatggta tgattattcc
     1441 tgagaatacg ggataatgat ctgaatatgg tactttttga gttatgtggc tcatgctcca
     1501 gaatttttca ggatagagta tgtaatcaat tctgagcatt ggccaaagat atgaataagt
     1561 agcactaaag cctcttccac tctcacgaaa actgtctttc atacctttca ttagaatgtg
     1621 atatgtatag gacatggggg tgtcgttgaa atctccacat ataattgcag gatattcgct
     1681 tttagacata tgaacagcaa ttgaatctac ctgtttgcct ctttttttaa atgcacttgc
     1741 aactctttca tggacattaa taatctcttc tgatactttt tgactgttgc tcattctttt
     1801 gactaacgtg gtaaatgaga ttgaatgtga ctcaagatga gtattataga tccttatcat
     1861 tttaccaaag aaatcaatat ctgaaaagat gcaaagattt gtactgcctt tgaaagtaat
     1921 cttgtctcct gaaacaatcg ggaatttact aattgtaaga ttaccgaaca tccctccatc
     1981 gctgtatctg aaaaaatgcc actgtatata aggatattga tgaaacaaat ctcttataac
     2041 cgaggtgtct ttggcataaa attcctggaa acagacaatt gaaggtcgtt cttctccgat
     2101 atatctggca actctgttaa gttggtcaag tcgtgaaatt cctgattcct gagcaaacag
     2161 accaacgttg taagtgcata tattcagtgc gtttcccttt tctccttcgt ttggcttgcc
     2221 aaattttaca aaaagatcgg caaataaaat ggaagggata agcattaaaa aagtaaccca
     2281 ggccacagag cgacttctga tgagagcgat tacgagaata ataatattta aaaaaacaat
     2341 tggaataaaa tataatccaa aaaaaagtgg gattactgaa tcgacaggat ttatataaat
     2401 tgagatatat gaaagtccga gcgctgctgc agcaacaaac agcaatatcc tgaaagatat
     2461 tgatatcggc cacagtgatc tcttttttcc cttttttctc ttacgcatta gaaatacatc
     2521 ttattgtgtt ttttccaata taaaatcaga aagtatccaa atatcatacc tcctaaatgt
     2581 gcaaaatgag ctatatttcc tccggtgttt gatattccaa agaaaagttc aatggcgcca
     2641 aaaatcagca caaaccattt tgcttttaga gcaattggag gaaaaagaag ttggagcacg
     2701 ttgttaggga atagcattcc gtaagcaagt aataccccgt aaattgctcc tgaagctcca
     2761 acagtaggtg tggaaattaa tattctgtat ttctctgcag caactatact gccggcttct
     2821 gctgcttgca gaagagaagt tgcgtcaaaa taaagaacca gggagtgaag aaaggctgct
     2881 cccagtcctg taatcatata gtaaaggaaa aatttcttgc tgccccaaac ctgttcaagt
     2941 atcacaccga agaaaataag agagtacata ttgaaaaaga gatggataaa accaccatgc
     3001 atgaacatat gtgtaataag ctggtatggt ttgaacagtg gagaaccaaa atagaaaagt
     3061 gaaaactttt catacatgaa gttacctgta aatgctgtaa taaccaacat aagagtattg
     3121 attattacaa gatttttgat aacaggtgtc atgcctccaa ggaatccggt tcctcttcca
     3181 gtattgtagt acatattttt attatataaa acgtttatct atttcttcaa gtgttattat
     3241 tgttgaacaa ggtttaccag acggggtaaa agaaggttcc ctgcatgcaa acaatgtgtc
     3301 gattaacaat tgagcctgtt gattgttcat tgaatctttg ccgcaaagag ctgctgatag
     3361 tgccagagta gctgcatatt tctgtcttgc atcttcaagt tggctattcc ccagctctgt
     3421 caagtcatat acgagttgat ctatcagagc caaaaggtcg tctgtagagt cagaaatatt
     3481 ttcaggcaat cccatcactt caacaacgtt tctaggagag ccatcctctt ttggccgtat
     3541 gtcaaatccg agactttgca aaattcccgc attttccaaa attatgttat atgtgttttc
     3601 atccaattca atagattttg ggaataaagt ccgtattgaa attgtggaaa agttgttaag
     3661 agagtcgaga tacttttcgt aaaaaattct ttccctggct cttttaatat taataatcaa
     3721 caaacccgac ttaatggctg taattatgta tcttcctgca acttggacag tgggtctttc
     3781 gagtcttgcc gtttctccta caaaaggttc cattagttct ctcttatatt gaagagtttc
     3841 ttcaaatgga ttgaaaagag gatcaaagtt gatttttgga gggggagcat aataattgta
     3901 atttacagca gggatttcag gtgcgccttc tgtatcaaaa tctatgctcg gcattaaaga
     3961 atttcgccct agtgattctc ttactactgc atgaagcatg tcgtaaattg caaattcatc
     4021 ttcaaatttc acctctgtct tggaaggatg aatattaacg tctattcttt cgggatcaat
     4081 ttctaaaaaa ataaaaaatg aagggatagt ctcttctttg atcagatttt cataggcttt
     4141 gacaacagct ttctgaaaat aaggggatct gaaatatctg ttatttacaa agaaatactg
     4201 attcccagga gtttttctcg aatcttcggg ttttcctgta aaaccactta ttttgacaat
     4261 tggactttcg agatggatgt gtactaactc tttgcccatc tcttttcctg ctatgtcaag
     4321 aattcttttt ttaaggttgg ctggggagag gtggaatatt tcaatgtcat tatgtatgag
     4381 cctgagagaa agatcgggtc ttgtgaatgc tactcttgaa aattcctgga ctatttgacg
     4441 gaattcggtt gcatcggatt ttagaaattt ccttcttgcc ggaacattgt aaaagagatt
     4501 tcttacaata aaattacttc cttttggtgt agagatttct ttttctgata tcagtgaaga
     4561 ttcggcaaat ttaatttcac atcctatttc gtctgattct cttcttgttc tcaaggtgac
     4621 ttcagaaaca gctgccactg atgcaagtgc ttcacccctg aatccatatg tatttaaatg
     4681 attgagatct tcaatggttt caattttgga tgtggcatgc ctttgaaagg ctatcttagc
     4741 ttcttcacgc gacattcctg aaccattgtc ggtgacttga ataagagtcc gccctgaatc
     4801 cttgataata acttggatag aatctgctcc ggcatccact gcgttctcaa gcatctcctt
     4861 aactacagaa gcaggccttt gaactacctc tccagcagcg atgagatttg taatgtggcc
     4921 gggaagaatg tgtatcatta tttggaagag gtaagtataa accctaatcc atctgccaaa
     4981 taaaatgcta ttgcaaacaa tatgacaaga gaaatcaaaa taatcaatct tgtagtaagc
     5041 acttttcctg gatccttttt gtttttataa acatcattac gcatatgagt gcgaatgtac
     5101 ttgcctggta tatacttgcc atccttgctc tttccctttt cttcgttcag ctgtgcatat
     5161 ctttcttcca tcgcctcttt tttagggtcg tagtattgag gttcatagtg aaatacctta
     5221 tgtgatgtag gtctaaaaaa gttaaaagaa aagcccatgt taaatattct cctttaatga
     5281 aggtttcgac tgcaaagata acaatttaag taggaaaatt attatatctt tacattatgg
     5341 ctgtgagaat tggaaatgga tttgacgttc atcgcctgaa taacggactt ccattgtgga
     5401 tttgcggtgt caggattgaa cattcaaagg gatgtattgc tcattcagat ggcgatactc
     5461 ctattcatgc tctttgtgac gcgttgttag gctctcttgc tcttggcgat ataggcaaat
     5521 attttcctga cacttctgac gaatttaaga atattgacag caagatattg ctccacagag
     5581 tatatgaaat ggtcaaaact tctggatact cgctggttaa tgccgatatt acaattcttt
     5641 tacaacggcc caagttggca aattacattt ctcttatgag agagactctg gcaggagtta
     5701 tggagatcga ttcttccatc atttccatca aggcaactac aactgaagag ttgggattta
     5761 ttggcaagga agagggaatt gcagcttttg catcggtaat tgttgaaaaa ttggtttaac
     5821 actatttttt gaacaatttg tatcgtcatt tttttgtatt acttttgcac ctcaattgag
     5881 cggcgcgata gctcagccgg ttagagcgca tgattcataa tcatgaggtc cccagttcaa
     5941 tcctgggtcg cgctacggat acaatcaccc acttgcagag gcaggtgggt gatttttttc
     6001 agtctcaaaa tacattttag tcagcagttt agaatattag cattaaggtg ttataaatat
     6061 ttcctaaatt tgaaaattaa tatggtgaac acttctaaac ttaaaagtat tgtaatgccg
     6121 atagcactgg ttgtcggcat atttttgggt ttgacaattc caaaagcggt tatagtggca
     6181 aatgctgcaa tcccatattt gattggtctt atgcttcttt ttacatatag taaactgaac
     6241 ttcagagaat tgagtatttt tccaatgccg cttgtattaa tactcattca ggttggagct
     6301 tctatcattg cttatttggc tctttataga tggaatccgc tttttgctca gcaggcattt
     6361 atctgcctgt tgtgtcctgt ggctacttct gctcctgtta caacaaatat tctcggaggc
     6421 aatactgcct ctattgtttc ttatacattg ctctgttatt ttgtaatcgc acttgttttc
     6481 cctttattgc ttcctttggc tggaggtgtc ggagaggaga cttttctatc tacatctttg
     6541 gtaatagcaa agcagataat gccattaatt tttttgcctt taatagtagc tgtatttgta
     6601 agaaaaattt catacaaatt taattctttc ctcgttaacg ggcaggatat agctttttat
     6661 ttgtggggtt tgacgctgac tcttgtaata ggcaaggcaa caacatatgt tgcacaacag
     6721 ccctcaaacc ttattcctat ggagattggg ctggctctga ttgcacttgt ggcttgcttg
     6781 ctgcaatttg caattggccg ggaagttggt ctgcattata aggatgcaat tgctgcaact
     6841 cagggactgg gtcagaagaa cattgcactt gccatgtgga taggatttac atatctcaat
     6901 cctctcgtgt cagttggact ggctgcttac agtatatggc agaatattat taattcagct
     6961 caaatttatc ataaaacaaa ataaaaacat gaaaagcaga gattactatt ttcaattgct
     7021 gaaagctaat gtgcataatc ctaaaatgat tgcccacagt cttgcttccg aggcggttat
     7081 gagggctctt gctgagcgtc tggaagaaga gaaggattta tggggaattg ctggtttgct
     7141 gcatgatatt gatgtggagt taaccgcagg tgactcttgc cgtcatggtt tggaaggagc
     7201 aaaaatgctt cagaatgagg atttaccgga agaggtgctt gatgccatta taatgcataa
     7261 tgaaaaggct tcaggcaagg agaggactac aaaatttcaa catgcactgg ctgcaggtga
     7321 aacaattaca ggcttcatct ttgctatagc tcttgtgtat cctgataaga aaatttcgag
     7381 tgttaaagtt aaatctgtgg tcaagaggat gaaagaaaaa atatttgcgg catcagtcaa
     7441 acgcgaaaat atcagagagt gtgaatgtct tggattgcag ctggaagaat ttgtcgagtt
     7501 gtctctcaat gcactttctc ccatagagag tgaacttggt ttttaatttt taactttgaa
     7561 taaatcaaaa tataattgtt tgaaaatgaa aagagcagta ttatttctta catgtttgtt
     7621 tacctcttgc tttgcaatgt tgggccagca aataggaact tctgaattaa aagagattaa
     7681 atctggtttt atcaaagacc agaaaacaac tgcaattcag aactttgtct cttctgacaa
     7741 ggattttaaa agtaaaactc tcaacaggga gaatcagggc aaagtcgatc atttttttaa
     7801 gtacagggtg aatgttaagg gtattacaaa ccagcaaaat tcaggtcgtt gctggatgtt
     7861 tacctctatg aatgttttaa gacctgttgt aatggataaa tattctcttt ctgaatttga
     7921 cttttctcat aattatagtc agttctggga tctttttgag aaaagcaatc tttttcttga
     7981 gaatgttata gccacagctt ataaagattt tgacgacaga gaggtggcga gatatttttc
     8041 atctcctgtg gatgatggtg gcgtttggaa tcttttttac aatgttgcag agaaatatgg
     8101 tgtagttcca cagtcagtga tgcctgaaac tgcaaattcc gacaataccg gacagatggt
     8161 tggagtgctc aatgagagac ttcgtgctgg aggttacgcg ataagggaat taacaaagtc
     8221 cagttcaaaa tcgaagtctg atagtggaca aatagaaaga gagatgcgaa acgagaagat
     8281 aaatgtgatg aaggatgttt acagaatcct ggcgatttgt ttaggtgagc cgcctgcaca
     8341 gtttacctgg aggtataaga caaaggaggg aataataaaa tctataacta ccactccatt
     8401 ggatttttac agagggattg tgcctcagga ttataatcct aaaacattta ttatggtgat
     8461 gaatgatcca acccgagaat attacaaact ttatgagata aaaaattata gaaacacaat
     8521 tgaaggcatt aactggtgtt atttgaactt gccaaatgat gagatcaaag gctcggctct
     8581 tgcttcaatt aaaaataacg agccacttta tgcctcatgt gatgttggta agcaacttga
     8641 cagggaacat ggaattttag atgttaataa ttatgactat gaatcactgt ttggcattaa
     8701 tatgagaatg gacaaaaaag ccagaatact tacaagacaa agtggatcta ctcacgccat
     8761 gctgcttatt gcctgtgata cagacgataa agataagcct ttaaagtggg agtttgaaaa
     8821 cagttgggga gcttcagcag gacataatgg ttatttaact cttaccgatg attggttcaa
     8881 tgaatatatg ttcagagtag tgatcaataa aaagtatctg agtcagaagg caatagatgc
     8941 tcttggacaa aagcctgttg aactgcctgt gtgggactat atgttctaaa tgatttgctg
     9001 attacagcat ttcttcgatc tcttttatcc cttctcgatt gaagacaacc cgatatcgct
     9061 tgtaagactc aaagtcgaga tacttaagtt gtattataaa atttagataa tacactttag
     9121 ctcctaatgt ttgtatgagt tctccttttt cgtttatgca aaagagaatt gtttcgggat
     9181 tatccatctt gctaagaaag gattgaacgt tgaaacggat gatttcattt aagccagaca
     9241 caaaatactt actgctttca tcgagtttgg atctgtcaat ttgtaacagt tttctgtata
     9301 gcatgatctt ttcgtttgtg taacgattat cagcttcaag aagagccgaa cggtttcgga
     9361 tttgtaatat ctcttttgga actttttttt cagggataaa gtcaaaccct tccttgctga
     9421 agccgacaat gttatccttg atgctaacgg ttattttatt atcaaaatat tttgcttttc
     9481 ttttgtgagc aaaataatat ctcattaact ctttgattct gtctttaagc atgtaactta
     9541 caacaagtgc aacaaaaaga ggcatagtaa gattgccgta agtcctttgg aatgaaaagg
     9601 caacagctgt tgcaaatatc atggatatac ctgctgcgag actgaaataa atttgttcaa
     9661 caagaatacc gtcttttttc ttttttgcag tcaaaaaaag gtcactttcg acaaattttt
     9721 tgagtactcc tcttctaaat acaagatttc tgttgttatc agggctatca agagacacta
     9781 ccggataatc ttgattcttt ttatattgat tttctgcttc gacaagtgaa ataatctttc
     9841 ctctgtattc atctgctaat gtttggtcac tctcttccaa cttatgaaga agtgtaagaa
     9901 attgttggtc aacgatgttt gacataaatt catctccaaa ctcgaagtaa ttgtaacttt
     9961 ctttgcttac tgtaggagtt ttaataattg tagagaggga tctgtatttt gatagtgtct
    10021 ccctgacatc ttcaatcaaa gcatccagca agaatccctt atcttcaggt tgagcggatg
    10081 aaagaatata cttgatttga tctcttagag cacttttaaa gatagcagcg aacatcttta
    10141 tctgatattc gtaatccgca tttgagcttc gtgaaggata tgaagccagc ttatgaaaag
    10201 aagcttcaag atgtttcaga ggtatcatat ctccgcgtgc aatatccctc atcagaaaaa
    10261 tgggtgttat aagtcttatg ttgtctctaa tgtctctgta gaagtgttct ctcgggtatg
    10321 taagatgatt tatatcaagg ctgtctggta tgaatatcca ggtattcatc acaaagtcat
    10381 taatcttcac cttcctgcgt gcgtgaaaac caactttaaa ctctattgaa aagttatcat
    10441 gaatacttgc ttgaatatct atcatcagag aagtgtatat ttttcaaaat tacaataatc
    10501 cgtttgtgta tccaagccat ttgagaagtg tttcgcccca aatgagtatc atgacccagc
    10561 ttataatcat cattgtcatt ccaaacttaa aggtgtcact ccagctgtaa tggttagttg
    10621 tataaagtaa tgctgcaggt ttactgttaa aaggcagcac atagacatgt tcgcataaaa
    10681 gagcaacagg aaaggcaagg ctcataatag gatatccaaa cttttgtgcg actcctattg
    10741 caattggaac aaaaatgaga gcgcgcatgg tttttgactg aaaaagcaat gctgagaata
    10801 acattgctgc tgtaaggatg gcataaagga cccaaaaagg tgtatttgcg cctattccaa
    10861 gattatcgaa tagttgtcct actaaagtgg atggtaatcc tgtcgcttct aagccggctc
    10921 cgagagtata tgctcctgca gaaaagagaa gaagatgcca cggaatatca acgtcattcc
    10981 atttaaccac cccgatgcca ggtaaaagag ctacaacagc accaataaaa gcaactgcag
    11041 tttgattaat tccatgttgt ttatctgttg cccacaatag aagaacacca acgaatattg
    11101 caatggcttt gtattcttcg aacttcattt tacccatact attcaattct tctctcaatc
    11161 tattcattcc tccttcaata gaaggtttct tctcattatc ctttaactga aaaatgattt
    11221 tcataccaac gaaccaacct attagaataa gaatcataca taaaggaaat gcagccacaa
    11281 accaatcttg ataactaaaa gtagttaatc ccatagagcc cgcaattagg gcagctgaaa
    11341 gaagattggc acccgaaccg gtaaggaatc cacttgcgcc aatatttatc tgaaatagat
    11401 tttgaagtac aagatttcta ccaaaattat ttctgttatt gcctcctgtg gcaccataaa
    11461 ttgcagcaat gaccatgaaa attggcaaaa gaatcgccgc tttagcagta gttgcagaaa
    11521 taaatgcaga taaaacaata ttaattacta taaagctgaa aatgaccatt gatgcattct
    11581 tgccaaattt taaaacaaac caaagggcaa atctcttggc aactttagtt ttaacaagca
    11641 tgctagcaag gatgaaagaa agtatgttaa gccacataac agggtggcct aactgtgcat
    11701 aggccacctt gtctgtagtt attcccgtga gaacaattgc aagaattaca ataagtgaag
    11761 tcaagtaatt tggtacaggt tcagttatcc ataatattat tgatgaaaca aaaacagcta
    11821 acatggcata gtttgctctt gaaaagccaa atgcccccaa ttctgaaaat cgttttgccg
    11881 catctccaga caaagtgccg ggatcaatat tgttcaaaaa agggatatcc actataaaat
    11941 acagaacaat gaatgcaata attgctagag gaaaccccat ttttgccatc catttttcaa
    12001 aagtagattt ctcaagtttg ggtaatttct cgattttata gttattcata tcgagcgggt
    12061 caaacgcctg aaccttagct tccatattac tctgttttaa agtttcacca ttttgtataa
    12121 ggatttttca ttaacatcga gttatagtat cgggcatcac cggtaacttc ttctccaagc
    12181 cagtcaggtt tttcaaaagc ttcgttctcc gaagaaagct caacttctgc aagagtcagg
    12241 ccttcatttt caccataaaa ttcatcaact tcaaatgtat gtggtccaac ctttatcata
    12301 taacgagttt tatctattat tcctggctca caaatggaga gtaattcttg tgcttcttca
    12361 acgctgatct ctttctccca ctcaaatctg cttgctcctg agttattgcc tatgccttta
    12421 actgtaataa aacctttgtc tcctttgact cttaccctta ctgttctctc cggaacagag
    12481 ctgaggtatc cttggataat tcttgtactt tttgatgcaa gatgtttgaa ttcacccttt
    12541 acgaggaatt ttctttctat ttcctgtccc ataatactat tcatttgcaa gttttttgtc
    12601 tgacatacct attactcttt tgatagcctg caggtaatta atattctcta cgccgtcaat
    12661 ttggatatct ttgattgttt ttttgatatc ttctatttct gttgattccg agctaagggt
    12721 cattactttt gctccattaa tcagtatagc accggcttca caaatagtat cgttaaccat
    12781 ataaccccaa cgctgtttat gtacgcgtac ggcttgaaga tctggatgtg ccttgatcat
    12841 taagataaac tcctcataag tgtaaaaatc cttatcgagt tttgttatgt caatatcaac
    12901 cataaaggct gggaatacct cttttctcag tatatctcct gaaattggga actctccctt
    12961 catcaatgga ttccattgct caagaccgtc aatactttga acgagtgtct taatatccat
    13021 ttttccgtcc cttatttttg tgttattgat gtcattagtg cgtgatataa tgtaaatttc
    13081 atcactgaat ctttcccaca ccttttctgg taccggaaca gataatcttg ccattcgtgc
    13141 tattgctttt gagaaatcct gtccaaaagt gcgaaactcg aatcttggtt tggagatttc
    13201 tccaataacc atcttttctt tatccatttt ttatttttta aaatattaat aattaacgta
    13261 cgcctgttgc atttgacctt cccattgatc caacactaaa gagaactatc tgagcggctc
    13321 cgtcagtgac tctgccaatt gtatttgggc tagctccggc agtaattttg gttgtaagat
    13381 tatcaaagaa atcccttgtt gccccatttg aatcaaatag tgtgtaacta aatcctttgg
    13441 ttggagacat tccaaaagtg aagccggtta cgccatttac atcttgtgta atgacaagat
    13501 aacctttagc aggaattgtt actccggtag ggattgtcca gttattactt gcatcggtag
    13561 aaccatcctt tcttaatttc caaccaccaa ggtctactgt agaagtcata tcgttgtaaa
    13621 gctccattga tttcgcattt ggatcacatt cattgacata tacaagaata ggcggtgcaa
    13681 cctttatctt ctgttcttcg gaaactgctt tgaatccctt taaattggta gcttcaatta
    13741 cagcgactat gcttgcatta tctgcctgac ctggaattac tgccgaaaaa attcttcctt
    13801 cactctcggt cattgtaact gattgctgtg tgcctgagtt gactttatac ttcagaacag
    13861 cttcagtaat accattcaga tctgttattt tacaagaaat tgttattgcc tgatttgcca
    13921 tcggagatga aggatttatt gtaatctttt caataactgc aggcccttca tactgttcat
    13981 ctttaacgca gcccggaatt aaaaacatgg ctgcaagtaa tgcaattaat atttttttca
    14041 tatttttttc ttttaaaatt attttgaatt ttttaaaaat cgatttcgaa tctaattccc
    14101 agcatatgat aatctactcc tgccttgcct ttggcatctg caactttagt ggggtctttg
    14161 gtagggttgc cgtcaacgtc gtatcccaca tacaacttac ccttgccgtt tgcatatctg
    14221 tcattgttgc tgtacatata gttcagcatg attttgacag aattgttaac atagtagttt
    14281 attccaaatg tgtagttttc acctgatcca ccaaaaatcc cgttactggt aagatcaaga
    14341 aaatcatatc ttcccacgag ttcaatatct ccccacgact tgccacgtgt tggttgtgtg
    14401 aattcaccat cttgcacatt gtattgctgt ttacctccaa aaagaagata agctgcctga
    14461 gcataccatc cgttaaagtg tttagtatct ttattggtta gtttactgct ctctttgata
    14521 taagtgtcat tcccaatgta ttccgactgg aatctaaatc catttttaaa acctgcaagt
    14581 tcaaatccat aaagaaaatc gtgatcagta ccgtcaatta catctgtgtc taaatacttc
    14641 tttctgttga tagaagttgc gtttcttgtg ctgaatcttg aaccgttgta atctgcggga
    14701 tctacatcgg atttaggaag gcggtaggag gcagcagctc caatatgaag tccataatta
    14761 gctttttcgt aaaaaggcat aattacgaat tttcccgtat atgagtttcc gctgcttctt
    14821 ccataatctt tgttgttatc ttcaacatat gttcttgttt cctgaccttc tacagcctgg
    14881 aagtgaattc ctccttctgc caaaagccat ttccagttgt atttggcttg cagccctatg
    14941 tgtcttgaag gaccaaaggt ctgtactacc attggacgct cgagcagtgc aagataacgt
    15001 gatgtcgtgg tacattccat agaaaaacct tccttaaagt tacctgcagt gaattcgaaa
    15061 ttgcgtagtc cagagtagac tagaattgca tctttcaact caaatgagcc atttgcaaaa
    15121 tcggtatcaa gttcaccata ccagtccttt gaaatctgtg ctttgactgc aaatcttgct
    15181 cttcttatag atacaccatc tccgatctta tcaaagtctt tattctcacc aaaaaatgtt
    15241 gctccgtcag cttgtactct gatatcaaac caaagtctgt agtcttgaga tggagattcg
    15301 aaaaccagga tcccgtttct ttcctcaact gcaagagggt tcctgttaac tttgaccccg
    15361 tactgattgt atctcacatt ttcctgaggt ttttcctgag cccaggttgc taagcttagc
    15421 cctgttgcta aggtaatcat tattaatttt ttcatattta tagtgtttat agtgatttaa
    15481 tgaactctat taaattttcg tctcttttta aattcatttt ctttctaagt cgatatctgc
    15541 aaacttcaac acttttgggg gttatactca ttattgatga aagttctttt gtggaaaagc
    15601 ccaggcgcaa aagtgtcgca atacgttttt cttgttctgt aagatttggg aaacgttcgt
    15661 gcagtatgga cgagaaatct ttgtgaagca actcgacttg tgagtataat tcatttatct
    15721 catgagagaa agctttttta tctattattt tcttttctaa tgttcggacg gcctctttaa
    15781 ttttttctat ttcatcaatg ttttcaagat gttttatttc attataaatc tcatccagaa
    15841 aattacgttg ctgtccaatc caaagagcat tgttaatcaa atccttcctt ttaatttcaa
    15901 gccttttgtg aagattatcg ttttcatttt gaattacttt tatttccata tcaacgatga
    15961 ctttttgcat ttcatttagt ctgtttatct cttcggctct tttaagtcga gttttttcat
    16021 aatctctgtt ttttgaaatg tagaagtata caattattgc aactaaaaga aacagaactg
    16081 taactattga aaaagttgta atatcgagac tttgcttact gctatctgca ataactgtcc
    16141 ttagtgttaa atttgtaaga aggaaagaga atgcaggtaa aagaacaatt gagagagcag
    16201 attttatcat ttctccctta tttaagtcta tactcttatg aattaatccg attcctatta
    16261 tagaggcgag agaaacatga actggagaga caggcgtagc tgccagtcca atcgccgaac
    16321 agacagaatt acttgagaaa agaagcatta caagtgatac cgacgccatg attataacac
    16381 tagtttccgt gttaatgtca aaaagcttaa ttgaaagagt tgatgctttt tctttgatag
    16441 atataaaata tagagccagc acaacaatta taaatatggc ccagacaata tattgactat
    16501 taataaatat acctgcaagg ttaatattca acataaggtc tattggttgt gtaagcgcaa
    16561 ttattataga accattgtta attgagaatg aaaatattgt caatattaga aaaacaataa
    16621 gcgatatgct taatattctg tttaatacta ttagatgcaa gtcaattttc ttaataaggt
    16681 atttaacagc aaaaaacaat ataatagata ttatgcaact tatgatagga gatattaccc
    16741 aggctattac actttgccag aagtacgtca aagcaaattt atttttcata aaaaccatgt
    16801 aggccataat agctccaaac agagaataga cataagagtt ccatttggag aataatgaaa
    16861 agatccacat tgcaatggcc gaagacagtg taattaaaag aattatcagg ggagttgtga
    16921 tttggaaaga atcattgaaa ttgtttgata tacccttggt gaaaggtaaa gaaaacagaa
    16981 tagaaaaggc aattacaaga ggaagatact ttaatttaag aatgccggat gaaaaagaat
    17041 gcccgattat aattgggaat tctcttaggc taagccacag tcccgttaca attactatga
    17101 ttaaaacttc tatgatcatt tccttaagct aattggtcta ttgctcagaa gctgcgttta
    17161 atggccataa tagaaagcag atctgcagcc ttttcggcgg attctgaaag agtttcgatg
    17221 tgaagggcaa aatatctaag atgaaatttt tcactaagtt tcaattgagg catctcatga
    17281 aatacttttc tttttaaagt ttttgattgt cgctcggtct ctt
//
LOCUS       DAOTQL010000020        17286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3210_length_17286_cov_5.714932, whole genome shotgun sequence.
ACCESSION   DAOTQL010000020 DAOTQL010000000
VERSION     DAOTQL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3210_length_17286_cov_5.714932"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="P5293_02440"
     CDS             complement(<1..555)
                     /locus_tag="P5293_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HRR48798.1"
                     /translation="MSYLQFNKDELVNLEYSLKREVLSTNHAGGYMNTTIVCCNTRKY
                     HGLLVVPIKKFGDDKFVLLSSLDETIIQHDREFNLGIHRYGDIYEPKGHKYIRNFEID
                     RVVSITYKVGGVVLRKDIMFPHKKEQILIRYTLLEAHSPTTLRLKPFLAFRNFHTLSK
                     ANYNVNKHYDLIENGVSFRMYEDFP"
     gene            complement(560..2560)
                     /locus_tag="P5293_02445"
     CDS             complement(560..2560)
                     /locus_tag="P5293_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRR48799.1"
                     /translation="MEELKLLKDDPWLIPYRKQILRRYQKYVIRRQDIAGYGKPLNDA
                     VNSYLYYGVHKVGKSWFFREWAPGATAIYLIGTFNDWKIDNRYSLTPQSNGNWELKLP
                     LDAVHHGDLFKWYVFWRGGEGERIPAYARRCIQDENTKIFSAQIWDPPHFKWSNPRIF
                     GNYSPLIYEAHIGMSSEKPEVASFNYFRESVLPRIVDLGYNTLQLMAIQEHPYYGSFG
                     YQVSSFYAVSSRFGTPEDLKRLIDTAHGYGIAVVMDLVHSHAVSNIYEGLGLLDGNPS
                     LYFHEGQRGNHPAWGSKCFNYGKDEVLRFLLSNCKYWLEEFNLDGFRFDGVTSMIYLD
                     HGLGKNFTNYSFYYDGNQDEDALVYLSLANALIKEINPNAITIAEDMSGMPGLAAPIS
                     SGGNGFDFRLSMGVPDHWIKWIKELKDEEWNMSNIYYELTNKRADEKSISYCESHDQA
                     LVGDKTIIFRLIDKDMYYHMSKESSNLKVDRGIALHKMIRLVTLATAGDGYLNFMGNE
                     FGHPEWIDFPREGNGWSYHYARRQWSLADDPKLKYSYLLQFDKTMIAMAKLNNLFFDK
                     PVSIYEDNEKQILIFSRAGLIFVFNFNPTVSYFDYRFKVPAGSYKIILNSDSILFGGF
                     NRIDDDIIYDSFYDNESALLSLYLPSRSAIILKEFLNFKEKF"
     gene            complement(2572..2757)
                     /gene="xseB"
                     /locus_tag="P5293_02450"
     CDS             complement(2572..2757)
                     /gene="xseB"
                     /locus_tag="P5293_02450"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRR48800.1"
                     /translation="MKKSDIDYRTALEELQKIVEKIEDPNTDLRDISKYVKRANELIK
                     RCRELLREIEDNLPKIT"
     gene            complement(2744..3871)
                     /gene="xseA"
                     /locus_tag="P5293_02455"
     CDS             complement(2744..3871)
                     /gene="xseA"
                     /locus_tag="P5293_02455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRR48801.1"
                     /translation="MVKDKIITLSDLQNEIKDSIEQRFTDRYWITAEISEIKNHYAGH
                     CYMDLIDRKEGEDSVSAKAQAIIWANSYRIIRPFFETTAGQPLSKGMHVLLCVQVQFS
                     SLYGLSLVVTDIDPTFSIGEQELKRQQTIKRLKDEGMLNMNSSLPLPALPKRIAVISS
                     EQAAGYRDFIKQLQGNEFGFRFCIELFQAPMQGADAPEGIISAMDRVAARVDDFDLLC
                     ILRGGGSAHDLACFDDYDLAVNIAQFPLPVIVGVGHDHDYHIADMVAHTSVKTPTALA
                     DFLIDIFASEEQQLLYISRRVSMALQNRITEEKSKLQILEHRIIAGSPFALLERGYAL
                     LHKNGKRVTDIDEILVGDEVDAFLKGGKLKCIVKSKENEKE"
     gene            3942..4529
                     /gene="yihA"
                     /locus_tag="P5293_02460"
     CDS             3942..4529
                     /gene="yihA"
                     /locus_tag="P5293_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRR48802.1"
                     /translation="MKITKAIFSGSSRFAKDRPKPRLPEFAFIGRSNVGKSSLINMLC
                     NNKSLAHTSSKPGKTQLVNHFMINDRWYLVDLPGYGYAKLPKKTRIELDEIIRSYING
                     SEELVTLFVLIDCRHDILPNDLRFLMQLGEAEAPFSIIYTKSDKLSATALEKQISNNN
                     QILLEYWEELPPVFISSAVKGTGKEEILSYIETFI"
     gene            4545..5495
                     /locus_tag="P5293_02465"
     CDS             4545..5495
                     /locus_tag="P5293_02465"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRR48803.1"
                     /translation="MDHTHKIQIFSCRSSRYLAEKIAASLNLELGKSNVLVFSDGEFQ
                     PAFEESVRGATVFIIQSTFPPVENMFELLLMIDAARRASAYKVNAVIPYFGWARQDRK
                     DRPRVPIGAKLVANLLAAAGCDRVMTADLHADQIQGFFDFPVDHIYASSIFLPYLREL
                     NIENMSIAAPDMGGAKRANAYSRILGCPLIICHKSREKANVVGSMTAIGEVEGRNIVL
                     IDDMIDTASTITKAADMLIEKGALSVRALATHAVMSGPSYDRIAKSKLTEVILSDTIP
                     LSTDPNLDTSKIKILSVADIFADVIEKVYNNKSISTSFVF"
     gene            5509..6336
                     /gene="nadE"
                     /locus_tag="P5293_02470"
     CDS             5509..6336
                     /gene="nadE"
                     /locus_tag="P5293_02470"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRR48804.1"
                     /translation="MTPNLLHEKIVETLKFFMKSAMKDKAVVGLSGGIDSAVVASVAV
                     DALGPSSVTAILLPSPFSTLHSVSDAVELAENLKIQYYTVPIDGIFHKFQRELTDLFG
                     EEPCRLTTENLQARIRATILMAFSNQTGALLLNTSNKSELSMGYGTLYGDLCGAIMVL
                     GDIYKSDVYELAGYLNSLERRIPDSIITKEPSAELSVGQKDSDSLPPYPVLDPILYLL
                     NENGMSPQEIINSGADSPIVNKIVKMKDSASFKIAQLPPVIQIGQHPLLPDSKCIKL"
     gene            6366..6605
                     /locus_tag="P5293_02475"
     CDS             6366..6605
                     /locus_tag="P5293_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48805.1"
                     /translation="MQLLFFTIVLVALCVFLLSFNIIFRKNGKFPDSEIGHNKEMKKL
                     GIKCAKAEEKELWGVNGTMRKECPSDTCSNCRGCN"
     gene            6605..7912
                     /gene="der"
                     /locus_tag="P5293_02480"
     CDS             6605..7912
                     /gene="der"
                     /locus_tag="P5293_02480"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRR48806.1"
                     /translation="MGIIAIVGRPNVGKSTLFNRFVGRRQAIVDEISGVTRDRHYGSC
                     EWCGREFSVIDTGGYTINSEDLFEEEIRKQVLLAIDEADVILFICEVETGITDYDSQI
                     ANMLRRCGKPVVLAVNKVDTGDRFFDVHQFYKLGLGDPFAISSASGSGTGDLLDRITD
                     LLPANNKGEDKNDLPRFTIVGKPNVGKSSLANALIGEDRNIVTPVAGTTRDSISTYYN
                     QFGHEFMLIDTAGLRKRNKAMEDIEFYSVMRSIRSIENSDVCIMMVEAQKGIESQDMS
                     IFNLIVKNKKGCVLVVNKWDLVENKDSNTMKRMQEEIMSKIAPFNDIPIIFTSIINKQ
                     RLLNVLDAASKVYENRSRKIQTSKLNEVMLEEIENYPPPSVKGKYIKIKYITQLPTPS
                     PSFAFFCNLPQYVREDYRRFLENKLRKHFEFTGVPIQIYFRQK"
     gene            complement(7884..11492)
                     /locus_tag="P5293_02485"
     CDS             complement(7884..11492)
                     /locus_tag="P5293_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR48807.1"
                     /translation="MKEWLRKLSNERIAIYWIAVLVLTLASFIRFSSINSMDRAVTNL
                     QEKIHKRQQILENYARQAHEIPDSQFISFEDFPEDMVIYRYLDDTLQSWVNRLPIANE
                     EVFYFPYGYRLQHVGVENVSNTPLAYAFYSALANGNPEEEQYLSLGTGWYIVKMYISG
                     KMLIVTALLIQTDYPTENSLLRNQINPNILSNKRLSIVPVTFDESYVIHGKDGGVLFS
                     VLKDLPQSGERSGSVLRLTALVFVVLSLFAQLKKTKKIRDFFLLIGGLITVGVFAMYL
                     SNKMQSDLPLFSPTLYADFELFSSLADLLIVNLLITLIITALFIMRKTLALIVKKSKG
                     LKKRLFTALFILIPLLLLPYIHISLRSVILNSNVVLELYRVDEITFYSLLVYLSYALL
                     FVALLFSLQLLRPLSKKTRKISFLKTRNLIIYIICISFYTFFVVSFYGFKKEDNRNRV
                     WSNKLSTERDLNLELQLKTIEKQIISDSKLRLLVNAFGGEDLVHSQLSENYFWNILQR
                     YDMEIRICREYTTLQIDPDAPPVLCNSFFQNEVTRYGSPLYESSQFFFMNNYKGRVRY
                     LGIFEYPAIGESGLVRLFIELNSKFIKETIGYPSELLSHSQIDDFSLPYLYSYGKYVN
                     GRLVVSSGDYSYPIVSKKYDNEGYYNVRSDGYLHFVSRVTPETVIVLSRKERGIFPYF
                     VSLSYIVLFYFLIIFGLVHRRRERNVLKKLPKNTFRWKISMLLLSSLVFALLFMGTGS
                     VWFSINYFNENTKIQLEEKMSSVQSTLSLFSQYVSRYNDPRFNNIQLQQAMNRLSSTA
                     GIDINLYSSAGFLIRTTQPEIFDRSILGKRMDDRAYIEIVKNNKKEFVHKEKIAGVEY
                     NSLYAPLYNQDGALIAIVNIPYFSQWANMRSNASNIIATIINIYILLLLAAVFGGIAL
                     SNSISKPLAEIGKKMELLDISERPEHIDYQNNDELGILVSAYNKMVDDLEESTKRLTE
                     SEREQAWREMARQIAHEIKNPLTPMRLSIQHLVRMKRQGVPDWPDKFDALANTLIEQI
                     DILSETAEEFSSYSRFYSEELSMVELNTLIREQMFLFNTRDDIQLKFNSEIAVAEINT
                     RKTQLTRVLVNLLSNAVQAIPNQESGKIYVSLTMEENYYQINVEDSGAGVPENLTNRL
                     FKPNFTTKTGGTGLGLAICRSIIEQSNGKISYMRSELLGGAKFSVKLPISDESISV"
     gene            complement(11489..11965)
                     /gene="rlmH"
                     /locus_tag="P5293_02490"
     CDS             complement(11489..11965)
                     /gene="rlmH"
                     /locus_tag="P5293_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRR48808.1"
                     /translation="MKIMLLLIGKSDASWLRQAIEIYEERLSHYVDYRRVEVPEIRNV
                     AGLSIMQMKEKESELLLKHIKPSDKLVLLDEKGEVYSSEEFANYIDKQLLSGTRNLVF
                     AIGGPYGFSELVYNRADSLLSLSKLTFTHQMVRVIFLEQLYRAFTIIRGEQYHHKQ"
     gene            12057..12455
                     /locus_tag="P5293_02495"
     CDS             12057..12455
                     /locus_tag="P5293_02495"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRR48809.1"
                     /translation="MNIFLFAIISSFFTFSSPATQYGSEQDVFVPIAKYIQSGDAEKL
                     SAWFAPNLEVDILGTVNVCSKTQAKQIIKEFFANNSPKSFVIEHRSGKAPLKYAIGTL
                     SAGGSKFRVTLFVKTQEDGNFIQQIRIEME"
     gene            complement(12473..13636)
                     /locus_tag="P5293_02500"
     CDS             complement(12473..13636)
                     /locus_tag="P5293_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR48810.1"
                     /translation="MKILIIDDEKSIRNTLKEILEFEGHEIILANDGLEGLNAAISTN
                     FDAIFSDIKMPNMDGLEFLDKAFEAGVESPVIMISGHGSIDTAVECIKKGAFDFIQKP
                     LDLNRILITLKNATEKSDLVRETKNLKKRVAKIQEIVGVSPAITKIKNMIDRVAPTEA
                     RVLITGSNGTGKELVARWLHEKSGRNNMPFVEVNCAAIPGELIESELFGHEKGAFTSA
                     IKQHKGKFEQADGGTLFLDEIGDMSLASQAKVLRVLQENKLSRVGSDKDINVKVRVIA
                     ATNKDLQEEIRKGTFREDLYHRISVIIIHVPPLCERVEDIPILTRYFIDQICEESGMP
                     PRKIDDDALEELKKYKWTGNVRELRNVTERLLILSNNVITKEDVINYARPVFK"
     gene            complement(13654..14568)
                     /locus_tag="P5293_02505"
     CDS             complement(13654..14568)
                     /locus_tag="P5293_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48811.1"
                     /translation="MKVAILDLGTNVFNLLLSEISGKTYKILKVVKIPSFIGAGGFNE
                     GRLSGEAMSKSIDALGKIREIIDEEGDVGFIKVFATSAFRDTVNGSEFANTIRDNFGF
                     EVEIISGEREAELIYKGIRESFFVWDENVLMCDVGGGSNELIIADKEKILWKRSFNLG
                     ILRLKEKFEPSDPIKEEEVKMINLYLEQELEPLFEACRQYSPRLMIGSSGSFDTLREL
                     LYTDDNGTLPVHELDIKRLRVLHHKLLVSNRGERLRMPGMSPVRVDYMVLGSIFIQLV
                     IEKCRISKLYQSSYSLKEGFMAEMAAQL"
     gene            14626..15321
                     /gene="cmk"
                     /locus_tag="P5293_02510"
     CDS             14626..15321
                     /gene="cmk"
                     /locus_tag="P5293_02510"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRR48812.1"
                     /translation="MDSPKIIIAIDGHSSTGKSTFAKTIAKKFGLLYIDTGALYRGIT
                     LFAIQNGLITSDNKVKEAELKSLLPSVSMEFRVTGKDCESELYLNGKMVENEIRSLDV
                     SSKVSYIAALPFVRNFVDEILRKYGKIRGVVMDGRDIGTVVFPDAEIKIFMTADAEVR
                     AKRRYDEMIARGANADFNEILKNIIERDYIDEHRETDPLRKAPDALLLDNSHMTVDEQ
                     MEWLLSKISQKWN"
     gene            15312..16178
                     /locus_tag="P5293_02515"
                     /pseudo
     CDS             15312..16178
                     /locus_tag="P5293_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
     gene            16236..>17286
                     /gene="priA"
                     /locus_tag="P5293_02520"
     CDS             16236..>17286
                     /gene="priA"
                     /locus_tag="P5293_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRR48813.1"
                     /translation="MVPTELQKEVSKGTLVRVNLSNREYYAVISELSHSHGTFKGEIK
                     NILSIIPSFKITENEFKLWEWIAKYYLCTSGEVFKAACPSFSIDKKPRKRNREIISRE
                     KENLPELSDAQESALKSVKQSFADGKITLLYGVTGSGKSEIYMHLAMEELKKGNNVLF
                     LVPEIAMSRQLGTRLERFFGNSLLIYHSKQSLSERERVRKQLYDHKCPFIVLGTRSSL
                     FLPFTNLSLIIVDEEHDSSYKQSDPAPRYSGRDTALILASLHSANALLGSATPSLESY
                     FNAISGRYSMVELSERYYGSVNPEIEIIDVVREKKISRMKGPFSKKMIAAIKDTLEEG
                     RQVMVFRNRRSYSPMV"
BASE COUNT         5497 a         3407 c         3039 g         5343 t
ORIGIN      
        1 aggaaaatct tcatacattc taaaacttac tccattttca atcaaatcat aatgtttatt
       61 gacattgtaa ttggcctttg aaagagtatg aaagtttctg aatgcgagga atggtttcag
      121 tctcaatgtt gtaggtgaat gagcttcgag aagagtatat ctaatcaata tctgttcctt
      181 tttgtgagga aacatgatat ccttacgaag cacaactcct ccaactttat acgttattga
      241 cacgactctg tcaatctcaa aatttcttat atacttgtga cctttaggtt catagatatc
      301 tccgtacctg tgaattccaa ggttaaattc tctgtcatgc tggattattg tctcatcaag
      361 tgaggaaagt aatacaaact tatcatctcc aaatttcttg ataggtacaa ctaaaaggcc
      421 atgatatttc ctggtattac aacaaacaat tgtggtgttc atataaccac ctgcatgatt
      481 agtggacagt acttccctct taagagagta ctcaagatta accagttcgt ccttgttaaa
      541 ttgtaaatat gacatatctt taaaatttct ctttaaagtt aagaaattcc tttaaaataa
      601 tagcactccg tgaaggaagg tataaagata acaatgccga ttcattatca taaaatgaat
      661 catatataat atcatcatca attctgttaa acccgccaaa cagtatggaa tcagaattaa
      721 ggatgatttt ataactacct gcaggaactt taaatctata atcaaaatat gagactgtgg
      781 gattaaaatt aaaaacaaag attaatcccg cccgtgaaaa aattaaaatt tgcttctcat
      841 tatcctcata tatagaaaca ggcttatcaa aaaaaaggtt attaagtttc gccattgcaa
      901 tcatagtctt gtcaaattga agaagatatg aatacttcaa tttaggatca tctgcaagcg
      961 accactgtct tctggcataa tgatatgacc aaccattccc ttctctgggg aaatctatcc
     1021 attcaggatg gccaaattca tttcccataa agttgagata accgtctcct gcagttgcaa
     1081 gtgtgactaa acgtatcatt ttgtgaagag caatgcctct atcaactttc aaattggaac
     1141 tctccttact catatgatag tacatgtctt tatctataag tctgaatatt atggttttat
     1201 ctccaacaag agcttgatcg tgactttcgc agtaacttat actcttctcg tcagcacgtt
     1261 tattggtaag ttcataatag atattgctca tattccactc ttcatctttc agttccttta
     1321 tccacttaat ccagtgatcc ggaactccca tgctcaacct gaaatcaaat ccgttacctc
     1381 cggatgatat gggagcagcc aatcctggca ttccgctcat gtcctcagct atagttatgg
     1441 cgtttggatt aatctcctta atgagagcat tggcaaggga aagatagact aatgcatcct
     1501 catcctgatt cccatcataa taaaaagaat aatttgtaaa gtttttcccc aatccatgat
     1561 caaggtaaat catgctcgtc acgccgtcaa acctgaaacc atcaagattg aattcttcta
     1621 accaatattt gcaatttgat agaagaaaac gtaatacctc atctttgcca taattaaaac
     1681 atttcgagcc ccatgcagga tgatttcccc tttgtccctc atgaaaatag agcgaaggat
     1741 tgccgtcaag cagcccaagc ccttcataaa tgttgctgac agcatgagaa tgtactaaat
     1801 ccattactac tgctattcca tagccgtgag ctgtatctat aagtctcttt aaatcttcag
     1861 gagtaccaaa acgagagctt actgcataaa aactcgatac ttgataccca aatgacccgt
     1921 aataaggatg ttcctgaatg gccataagct gcaaagtatt ataacctaag tcgacaattc
     1981 gtggcaatac gctttctctg aaataattaa aactcgcaac ttcaggcttt tccgaactca
     2041 taccaatatg cgcttcatag atgagaggtg aatagttgcc gaaaatacga ggattagacc
     2101 atttaaaatg tggtggatcc caaatctgag cagagaatat tttggtattt tcatcttgta
     2161 tgcaacgacg ggcatatgca ggaattctct ctccttctcc acctctccag aacacatacc
     2221 atttaaaaag atctccatga tgaacagcat ctaaaggtaa tttgagttcc caattgccat
     2281 tgctctgagg agtaagagaa tatctgttgt caattttcca atcattaaaa gtcccaatga
     2341 gatatatggc tgtagcacca ggtgcccatt ctctaaaaaa ccaggattta cccactttgt
     2401 gcactccata ataaagataa gaattgacag catcattaag aggcttgcca tatcctgcta
     2461 tatcctgtct tcttataaca tacttctgat atctccgaag tatctgtttt ctgtaaggta
     2521 tcagccaagg atcgtctttc agcaatttta gttcttccat aaaaccagat attatgtgat
     2581 tttcggcaaa ttatcctcaa tttctcttag aagttctctg catcttttga tcaattcgtt
     2641 tgccctctta acatactttg atatatcccg aagatctgta ttgggatcct ctattttttc
     2701 tactattttc tgaagctctt caagagctgt tctgtaatct atatcactct ttttcattct
     2761 cttttgattt aactatacat ttaagtttcc ctcccttaag aaatgcatct acctcgtctc
     2821 caacaagaat ttcatcgatg tcggttactc ttttgccatt tttatgcaac aatgcataac
     2881 ctctctcaag aagagcaaaa ggactacctg caataattct gtgttctaaa atttgaagtt
     2941 tactcttttc ttccgttatt ctattctgta aagccattga gactctgcgt gaaatataaa
     3001 ggagttgctg ctcctcagag gcaaatatat ctattaaaaa atctgctaat gccgtaggag
     3061 tcttgactga agtatgcgcc accatgtcag ctatatgata atcatgatca tgcccgacac
     3121 caactattac aggaagagga aactgggcaa tattgacagc cagatcataa tcatcaaagc
     3181 aagcaaggtc atgcgcagaa ccacctcccc tcaatataca caaaagatca aaatcatcaa
     3241 ctcttgctgc cactcgatcc atcgcactga ttatcccttc aggagcatct gcaccttgca
     3301 tcggtgcctg aaacaattca atacagaacc taaacccaaa ttcattgcct tgaagctgtt
     3361 tgataaagtc cctgtatcct gctgcctgtt ctgaagagat tacggcaatt ctttttggta
     3421 atgccggtaa gggcaatgag gaattcatat ttaacatccc ttcatccttc aatcttttaa
     3481 tagtctgctg ccgtttaagc tcctgttcac caatcgaaaa tgtgggatca atatcagtaa
     3541 ccacaagact taatccatat agagatgaaa attgaacttg gacacaaaga agtacatgca
     3601 taccttttga aagaggttgt ccggcagtag tttcaaagaa tggccttatg attcggtatg
     3661 aattagccca gattattgcc tgagctttag cagagactga atcctctccc tcctttctgt
     3721 caattagatc catataacaa tgtcctgcat agtggttttt tatctcacta atctctgccg
     3781 taatccagta tctgtctgta aatctttgct ctattgaatc ttttatctca ttttgcagat
     3841 ccgataaagt gattatctta tcttttacca taatcaactt tagttattcc ctatgcaaaa
     3901 gtaagcaata aagttgtacc tttgtagagt atttatccca tatgaagata actaaagcaa
     3961 tattctccgg aagcagccgt tttgccaaag acagacctaa gccgagactc cctgaattcg
     4021 catttatagg aagatcaaat gtgggtaagt cttcccttat aaatatgctt tgcaataaca
     4081 aatctcttgc ccatacgtct tcaaaacccg gaaaaactca acttgtcaac cattttatga
     4141 ttaatgaccg atggtatctg gtcgatcttc cgggatatgg atatgcaaaa ctgcccaaga
     4201 agactcgaat agagctggat gaaattataa gaagttatat taatggttca gaagaattgg
     4261 tcactctttt tgtcttaatt gactgccgac atgatatttt accaaacgat ctcagattcc
     4321 tcatgcagct gggagaagca gaggctccct tctccatcat atacacaaaa agcgacaaac
     4381 tgagtgctac tgctcttgaa aaacaaatta gcaataataa tcaaattcta cttgaatatt
     4441 gggaagaact cccacctgtt tttatcagct ctgcagttaa aggcacagga aaagaggaga
     4501 tattaagtta cattgaaact tttatataaa taataaaaaa aaacatggat catactcata
     4561 agattcagat cttttcttgc cgaagctcca gatatctggc agaaaagatc gctgcttcct
     4621 taaatttgga acttggcaaa tcaaatgtac tggtattcag tgatggagaa tttcagcccg
     4681 cctttgaaga gagtgttaga ggagccactg tatttattat ccagtccaca tttccccctg
     4741 tggaaaatat gtttgagttg cttctaatga tagatgctgc aagaagagca tccgcataca
     4801 aagttaatgc ggttatacca tactttggct gggcaagaca ggatagaaaa gataggccta
     4861 gagtcccaat tggagcaaaa ttggttgcaa atctgctggc tgcagccggt tgcgacagag
     4921 taatgacagc cgatttgcac gccgatcaga tccaaggttt ttttgatttt cctgtcgatc
     4981 acatatatgc aagctccatt tttctgccat atttgcgtga acttaacatt gaaaatatgt
     5041 caattgcagc acctgatatg ggtggagcaa aaagagcaaa tgcatactca cgtattctcg
     5101 gctgcccatt gattatttgc cacaagtcga gagaaaaggc caatgtggtt ggttcaatga
     5161 ccgctattgg agaagtagaa ggcagaaata tagttttgat tgatgatatg atagatacgg
     5221 caagtactat cacaaaagct gcagacatgc ttattgaaaa aggtgctttg agcgtccgtg
     5281 ctcttgcaac tcacgctgtg atgtcaggac catcatatga tagaattgcc aagtcaaagc
     5341 tgacagaagt aattctttca gataccattc ctctgtccac tgaccctaat ttagacacat
     5401 ctaagattaa aattctgtcg gttgcagata tttttgcaga tgtgattgaa aaggtatata
     5461 ataataaatc gataagtact agctttgttt tttgatatgt taacaggcat gactccaaat
     5521 cttcttcatg aaaaaatagt ggaaacactt aaatttttta tgaaaagcgc aatgaaggat
     5581 aaggcagtag ttggcctctc aggaggaatt gactctgctg ttgtggcttc tgttgccgtt
     5641 gatgctcttg gaccatcatc agttacagct attcttttac cctctccttt ctctacgctg
     5701 cactctgtga gtgatgctgt ggaacttgct gaaaatctga agatccaata ttataccgta
     5761 cctattgacg gaatctttca taaatttcaa agagaactta cagatctgtt tggcgaagaa
     5821 ccttgcaggc tgacaacaga aaaccttcaa gcacgtataa gagctacaat tttaatggca
     5881 ttttcgaacc aaacaggagc tctgctgttg aatacttcaa ataaaagtga attatcaatg
     5941 ggttatggaa ctttgtatgg agatctttgc ggagctataa tggtgcttgg agatatctat
     6001 aagtcagatg tatatgaact cgcaggttat ctcaattcgt tggaaagaag gattcctgac
     6061 tctatcataa ctaaagagcc ttctgcagaa ctgagtgtag gtcaaaagga ttctgattcg
     6121 ctgccgccat atcccgtttt ggatccaata ttatatcttt tgaatgagaa tgggatgtct
     6181 cctcaagaga taataaactc aggagctgac agtccgattg tcaataaaat tgttaaaatg
     6241 aaagattctg catcattcaa aattgcacaa cttccgcctg ttatccaaat tggtcaacac
     6301 cctcttcttc ctgattcaaa atgcataaaa ttataaatat atttttctta ctaaactaaa
     6361 ttaatatgca actgctgttt tttactatag ttcttgtggc tctctgtgtc ttccttctct
     6421 cctttaacat tatcttcagg aaaaacggga aatttccgga cagtgagatt ggacataata
     6481 aggaaatgaa gaaactcgga ataaaatgtg cgaaagctga agaaaaagag ctatggggag
     6541 tcaacggaac catgcggaag gagtgtcctt ctgatacttg ctcaaactgc agggggtgca
     6601 attgatggga atcattgcaa tagttggacg gccaaatgtt ggcaaatcta cacttttcaa
     6661 caggtttgtt gggaggaggc aggcaattgt agatgaaatt tcgggcgtta caagagaccg
     6721 ccattatgga agttgtgaat ggtgtggaag agaattttca gtcattgata caggtggcta
     6781 cacaattaac tccgaggatc tttttgagga ggaaattaga aagcaggtgc tcttagctat
     6841 tgatgaagca gatgttattt tattcatttg cgaagttgaa acaggcataa ctgattatga
     6901 ctctcaaata gccaatatgt tgagaagatg cggaaagcct gtggtcttag ctgtaaataa
     6961 agtcgatact ggtgacagat tttttgatgt tcatcagttc tacaaacttg gtctgggtga
     7021 cccatttgct atttcgtctg cgagcggaag tggaaccgga gatcttcttg acagaattac
     7081 ggatttactt cccgctaaca acaaaggtga agataaaaat gacttacccc gtttcactat
     7141 tgtcggaaag ccaaatgttg gcaagtcatc tctagccaat gcgttgatag gagaagacag
     7201 aaacattgtc actcctgttg caggcactac tcgtgactcc atatcaacct actacaatca
     7261 atttggccat gaatttatgt tgatcgatac tgccgggctc agaaaacgta ataaagccat
     7321 ggaagacata gaattctact ccgttatgag atctatccgt tctattgaga attcagatgt
     7381 ctgtatcatg atggtagaag cccagaaagg tatagagtct caagacatgt ctattttcaa
     7441 cttgatagta aaaaataaaa agggatgtgt acttgttgtt aacaaatggg atttagttga
     7501 aaacaaggat tccaacacaa tgaagaggat gcaagaagaa attatgagta aaattgctcc
     7561 ttttaatgat atacccataa tctttacctc tataatcaac aagcaacgat tactcaatgt
     7621 tctcgatgca gcttccaaag tttatgaaaa cagatctcga aagattcaaa catccaaact
     7681 caatgaagta atgctcgaag aaatagaaaa ttatccacca ccttctgtta agggcaaata
     7741 tattaaaatt aagtacatta ctcagttgcc tactccaagc ccttcatttg catttttctg
     7801 caatttgcct caatatgtta gagaagatta cagaaggttt cttgaaaata agttaaggaa
     7861 gcattttgaa tttaccggag tccctataca gatatacttt cgtcagaaat aggcagtttt
     7921 acagaaaact ttgcacctcc caacaactca gacctcatat aagaaatctt tccattgctc
     7981 tgttcaatga tactcctgca tattgcaagt ccgagaccgg tacctcctgt ctttgttgtg
     8041 aaatttggct tgaaaagtct gtttgtaaga ttttctggca cgcctgctcc actatcctca
     8101 acattaattt ggtaataatt ttcctccatg gtaagagaaa catatatctt tccgctttcc
     8161 tgatttggga ttgcctgaac tgcatttgac agaaggttta ccaaaactct ggtaagttga
     8221 gttttccttg tattaatttc tgctactgct atctctgagt taaatttaag ttgaatgtca
     8281 tctcttgtat tgaaaagaaa catttgctcc ctgataagag tatttaactc aaccatgctt
     8341 aattcttctg aataaaatct tgagtaactt gaaaattctt cggctgtttc tgacaaaata
     8401 tcaatctgct cgatcaaagt attggctaag gcatcgaatt tatcaggcca atccggcact
     8461 ccctgcctct tcattctgac cagatgttgt atacttaatc tcattggtgt caaagggttt
     8521 ttaatctcat gagcaatttg cctggccatc tctcgccacg cttgctccct ttcactttcg
     8581 gtcagcctct ttgtgctttc ctccaaatca tctaccattt tgttataggc agaaacaagt
     8641 atgcccagtt cgtcattatt ttgataatca atgtgttctg gtcgctccga aatatcaaga
     8701 agctccatct ttttaccaat ttcggccagc ggctttgaaa ttgaattaga gagagctatt
     8761 cctccaaaga ctgcagccaa aagaagtaaa atgtaaatat ttattattgt agcaattata
     8821 tttgaggcat tgcttctcat attggcccac tgtgaaaagt atggaatgtt gacaattgct
     8881 atcagtgctc catcctgatt ataaagtgga gcataaagag agttatattc tactcctgct
     8941 attttttcct tatgtacaaa ttcttttttg ttatttttta ctatttcgat gtatgccctg
     9001 tcatccatcc tcttccccaa aatagaccga tcaaaaattt caggctgagt agtgcggatt
     9061 agaaaaccgg cagaactata taggttgatg tctattccgg ctgtgcttga aagcctgttc
     9121 attgcctgct gcaattgaat attattaaat cgagggtcat tgtatctgct aacatattga
     9181 ctgaaaagag aaagagttga ttgtactgaa gacatttttt cttctaactg tattttagta
     9241 ttctcattaa aataatttat tgaaaaccag acactgccag ttcccataaa aagcagggca
     9301 aataccaggg atgacaacag cagcattgaa attttccacc tgaacgtatt ttttggcaat
     9361 ttttttaaaa cattcctttc tctccgtctg tggacaagtc cgaatattat caaaaagtaa
     9421 aaaagaacta tatatgagag tgacacaaaa taaggaaata tgccacgttc cttccggctt
     9481 aaaacaataa cagtttcagg agtaactctg ctaacaaaat gaagataccc gtcacttcta
     9541 acattataat atccctcgtt gtcatatttt ttagaaacta ttggatagct atagtctcca
     9601 cttgacacaa caagtcttcc attgacatat ttaccatatg aatagagata tggcaacgaa
     9661 aaatcatcta tctgcgagtg gcttaaaagt tccgaagggt aaccaattgt ctccttgata
     9721 aatttggaat tcagttcaat aaagagcctt actaaaccac tttctcctat tgccggatac
     9781 tcaaatattc ccaaatatct tactcttccc ttatagttgt tcataaagaa aaactgagaa
     9841 ctttcataaa gtggagaacc ataccttgtc acttcatttt gaaaaaaact gttacataat
     9901 accggtggtg catcaggatc aatttgcaat gtagtatact ctctgcatat tctgatttcc
     9961 atgtcatacc tttgaagtat gttccaaaaa taattctcag aaagttgact gtgcactaga
    10021 tcttcaccgc caaaggcatt gacaagcagt ctaagttttg aatcagaaat gatttgtttc
    10081 tcaatggttt tcaactgcag ttcaagatta agatctcttt cggttgacaa tttatttgac
    10141 cacaccctgt ttctgttatc ttccttctta aatccgtaaa aacttactac aaaaaaggta
    10201 tagaaagaaa tacaaatgat gtatataatc agattacggg ttttaagaaa agatattttt
    10261 cttgtttttt tagaaagtgg tcttaggagt tgaagagaaa acaacaaagc gacaaagagc
    10321 agtgcataag aaagatatac aagcaaagag taaaaagtta tctcgtctac tctgtacagt
    10381 tcaagaacta catttgaatt gagtatgact gaccttaaac ttatgtgaat atatggaagt
    10441 aataaaagcg gaattagtat gaataaagcc gtaaacaatc tctttttaag gccttttgat
    10501 ttttttacaa tcaaggcaag agttttcctc atgataaaaa gagctgtaat aataagagta
    10561 atcaaaagat tcactattaa aagatctgcc aatgaactga acagctcaaa atcagcatag
    10621 agagttggag aaaatagcgg aaggtcactt tgcatcttgt tggaaagata catggcaaag
    10681 actcctacag ttatcagtcc gcctatgagc aagaaaaagt ctctgatttt ttttgttttc
    10741 ttaagttgtg caaaaagcga caaaaccaca aaaacaagtg cagtgagcct taatactgaa
    10801 cctgatcttt ctccgctttg agggagatcc tttaaaacag aaaataacac acccccgtct
    10861 ttcccatgaa taacataaga ttcatcaaaa gtgacaggaa ctattgaaag cctcttattg
    10921 gataagatat tgggattaat ctgatttcta agaagtgaat tctctgtagg gtagtctgtc
    10981 tggataagca atgctgtgac aattagcatt ttaccgctga tatacatttt aacgatgtac
    11041 caccctgtac caagactgag atattgctcc tcttcaggat tgccatttgc caaagcggaa
    11101 taaaaagcat atgctagagg ggtattgctt acattttcaa ctcctacatg ctgtaatctg
    11161 tagccataag gaaaatagaa aacctcttca ttggcaatag gcagcctgtt tacccaagac
    11221 tggagtgtat cgtccaaata gcgataaata accatgtctt cgggaaaatc ctcaaatgaa
    11281 ataaattgag aatcaggtat ttcatgcgcc tgccttgcat aattctccaa gatttgctgt
    11341 cgcttgtgaa tcttctcctg aagattcgtt acagcacgat ccatggaatt aatcgatgaa
    11401 aatctaataa aagaggcaag agtaagtaca agaacagcaa tccaatatat tgcaattctc
    11461 tcgttcgaca attttcttaa ccactctttc attgcttgtg atgatactgt tctcccctga
    11521 ttattgtgaa agctctatat aactgttcca aaaaaatcac tcttaccatc tgatgtgtaa
    11581 aagttaactt tgaaagtgaa agtaaactgt ctgccctgtt atacaccaat tcggaaaatc
    11641 catagggccc acctattgca aacacaagat ttctagtacc tgacagaagc tgtttatcaa
    11701 tgtaattggc aaattcctca gatgaataga cttctccttt ctcatcaagc aaaacaagct
    11761 tgtccgacgg cttgatatgc tttaaaagta attcactttc tttctccttc atctgcatga
    11821 tggataaacc tgcaacattc ctgatttctg gcacttccac tcttctgtaa tcaacataat
    11881 ggctcaatct ctcctcatag atctctatag cctgcctgag ccatgaggca tcactcttac
    11941 cgattaaaag caacataatc ttcattagga caaatgtaaa actttatttt caatatatat
    12001 accagagcag tggcttgatt tttgcttaag tttagagtgt cgtatataaa atgattatga
    12061 atattttttt atttgccata atctcttcat tcttcacatt ctcttctcct gcaactcaat
    12121 atggatctga gcaggatgtt tttgtaccta ttgcaaaata tatccagtca ggagatgcag
    12181 aaaagttgtc agcttggttt gcccctaatc ttgaagttga catacttggt acagtcaatg
    12241 tttgcagcaa gactcaggcg aagcaaatta ttaaggaatt ttttgctaat aactctccaa
    12301 agagttttgt aatagaacac aggagtggta aagctccgtt aaaatatgcc attggaactc
    12361 tcagcgccgg tggaagtaag tttcgcgtga cactttttgt gaaaacgcag gaagatggca
    12421 attttataca acagataaga attgaaatgg agtagtaatc aaactatcag tcctacttaa
    12481 atactggacg tgcatagttg atcacatcct cttttgttat tacattatta ctcagtatca
    12541 aaagtctctc tgtgacattt ctaagttcac gaacatttcc tgtccacttg tattttttaa
    12601 gttcctcaag tgcatcatca tctattttcc tcggaggcat gccactttcc tcacaaatct
    12661 gatcaataaa gtaacgagtt aaaattggaa tgtcttcaac cctctcacat aaaggaggca
    12721 cgtgaattat tatgacgctg attctatgat aaagatcctc tctgaaggtt cccttacgta
    12781 tctcttcctg caaatctttg ttagtagcag ctattactct caccttaaca ttaatgtcct
    12841 tatcgctccc cactctgctc aatttatttt cctgcaatac tctcaatact ttagcctgtg
    12901 aagcaagact catgtctcca atctcatcca aaaaaagtgt tcctccatca gcctgttcaa
    12961 atttaccctt atgttgcttg attgcagatg tgaatgctcc tttttcatga ccaaaaagtt
    13021 cactttcgat caactctccg ggaatggcag cacagttgac ttccacaaat ggcatattgt
    13081 ttcgtccact cttttcgtgc agccatctgg caacaagctc cttaccggta ccattggagc
    13141 cggttataag aacacgagcc tccgtaggag caactctgtc aatcatattc ttgattttgg
    13201 ttattgctgg tgaaacccct acaatttcct gtatcttggc aactcttttt ttaagatttt
    13261 ttgtctctct taccagatcg cttttttcgg ttgcattttt aagtgtaata agtattctgt
    13321 tgagatcaag aggtttttga atgaaatcaa aggctccttt ttttatacac tccacagctg
    13381 tatctatcga accgtgcccg gaaatcatta ttacaggaga ttctactcct gcttcaaatg
    13441 ccttgtccaa aaattcaaga ccgtccatgt ttggcatctt aatatccgag aaaatggcat
    13501 caaaatttgt tgaaattgca gcatttagtc cttctaaacc atcatttgcg agtattattt
    13561 catgaccttc gaattcaaga atttccttaa gtgtgtttct tatactcttc tcgtcatcaa
    13621 taatcaatat tttcattctt tctaaaattt atattacaat tgagctgcca tctcagccat
    13681 aaaaccctcc ttaagagagt aagaggattg atataatttg cttattcggc atttttcaat
    13741 taccagctgt ataaaaatac tccctagtac catataatca acccttacag gcgacattcc
    13801 cggcattctt aacctttcgc ctctatttga cactaaaagt ttatgatgca aaactctaag
    13861 tcttttaatg tctaactcat gtacaggaag tgtcccatta tcatcagtat ataaaagctc
    13921 cctaagagtg tcaaatgaac ctgaagaacc tatcataagt cttggagaat attgtctgca
    13981 cgcttcaaaa agtggttcga gttcttgctc aagataaaga ttaatcattt taacctcttc
    14041 ttcttttatt ggatcacttg gttcaaattt ctcctttaat ctcaatatcc caagattaaa
    14101 acttcttttc cacaatattt tttctttatc tgctataatt aattcattac ttcctccacc
    14161 cacatcacac ataagcacat tttcatccca tacaaaaaaa gattcgcgaa tgcctttata
    14221 aatgagttct gcttctcttt cgccagatat aatctcaact tcgaatccaa aattatcgcg
    14281 aatagtgttt gcaaattctg aaccatttac agtatctctg aaggcagatg tagcaaaaac
    14341 tttaataaaa ccgacatcac cttcttcatc aataatttct cttattttcc ccaaggcatc
    14401 tatggatttt gacatggcct ccccactaag cctcccttca ttaaaaccac cggctccgat
    14461 aaatgaagga attttgacta ctttcaatat tttatatgtt ttgccactaa tttcagagag
    14521 taacagattg aatacgttgg ttcctaaatc aagtatagca actttcatct actatttttt
    14581 tacaaagata aagctttttt gtatgtttgt tttcttaaga aagttatgga ttccccgaaa
    14641 ataataattg caattgacgg ccactcatct acagggaaga gcaccttcgc caagacaata
    14701 gccaaaaaat tcgggctgct ttatatcgac acaggtgcgc tttatcgcgg aataactctc
    14761 tttgcaatac aaaacggtct tataacatct gacaataaag taaaagaggc agaacttaag
    14821 tcgctcctgc catcagtaag catggaattc agagttacag gaaaagattg tgaaagtgaa
    14881 ctgtatctca acggtaaaat ggttgaaaac gagataagat cgcttgatgt ttcatctaaa
    14941 gttagttata ttgcagctct cccctttgtg cggaattttg tagatgaaat acttagaaaa
    15001 tatggcaaaa tacgtggtgt ggtcatggat ggaagagata taggcactgt cgtattcccc
    15061 gatgctgaaa taaagatttt catgacagct gatgcagagg taagggcaaa aagaagatat
    15121 gatgaaatga ttgccagggg agcaaacgct gatttcaacg aaattttaaa gaatatcata
    15181 gaaagagatt atattgacga acatagagaa acagaccctt taagaaaagc ccctgatgct
    15241 ttgctgcttg ataatagtca tatgactgta gatgagcaaa tggaatggct cttaagtaaa
    15301 atttctcaaa aatggaactg acagtagaaa tagatgccgg atctggttgc tgctatggtg
    15361 ttgtaagagc aatagaagaa gcggaaaaat atcttgaaaa aaacagtaaa ctctactccc
    15421 tcggctcaat cgttcataat agcaccgaac tggaacgtct taaaaaaaaa ggattgattg
    15481 tcgtttcaca cgaagagatg tcaaaactta aggactctgt cattctcata cgtgcccatg
    15541 gagaaccccc ttccagctat gaaactgcaa aaaagaataa tcttactgtt gtagactgta
    15601 catgtcccgt agtgcttcaa ttacaaaaaa ggataaagga acagtttgaa aaagcacaag
    15661 cgataaatgg ccaaatcatc atatttggca aaaaaggaca cgcagaagta aacggacttg
    15721 taggtcagac tgatgggaaa gccattgtcg ttgagagtat ttctgatctc gattcaatcg
    15781 attatacacg gccaattact attttttctc aaaccaccaa agagtttgaa gaatatagca
    15841 atatatgcag tcagataaaa aaaagaattg aacagacagg agcaactaaa gaagacttta
    15901 aatctttcaa tacaatctgc ggacaagtat cctcgagaca ccctcattta agagaatttt
    15961 cagcaaaaca ttccgttatt atctttgttt caggtaagga gagttccaac ggagctattc
    16021 tttttaattc atgcaaatct gtaaatccaa gatcatacaa aattgagagt gtagatgaaa
    16081 tagacttttc atggttccag gagggcgata ggataggagt ttgtggcgct acatctaccc
    16141 caaaatggct gttggaagag gttgctggtt ttataagagg agatatgttt gctaaagtta
    16201 ttcttccatt gaggtacaaa gcagaaataa catatatggt tcccacagaa ttacaaaaag
    16261 aagtctccaa aggcacatta gtcagagtga acttgtcaaa cagagaatat tatgctgtta
    16321 taagcgaatt atcacattct cacggcacat tcaagggaga gattaaaaat attctgtcaa
    16381 ttataccaag tttcaaaatt actgaaaacg aattcaaatt atgggaatgg attgcgaaat
    16441 attacctctg cacttctggt gaagtattta aggctgcatg tccatctttc tctatcgaca
    16501 aaaaaccaag aaaaagaaat cgagaaataa tatcccgaga aaaggaaaat cttcctgaac
    16561 tttcagatgc acaagagagt gctctcaagt ctgtcaaaca atcattcgct gacggaaaaa
    16621 ttactctgct ctatggtgta accggttctg ggaaaagtga gatatacatg caccttgcaa
    16681 tggaagaact taaaaaaggt aataatgttt tatttttggt gcctgaaata gcaatgagtc
    16741 gccaattggg taccaggctg gaacgtttct ttggaaattc gctgcttata tatcactcca
    16801 aacagagctt atctgaaaga gagcgagtca ggaaacagtt atatgaccat aagtgtcctt
    16861 ttattgttct tggcacaaga tcatctcttt tcttgccatt cactaatcta agccttataa
    16921 ttgttgacga agaacatgac tcgtcataca aacaaagcga tccagcgcca cgatacagtg
    16981 gaagagatac agctttgata ctcgcctctc tgcactctgc aaatgcactt ctcggttccg
    17041 caacaccttc tctggaaagt tactttaacg ctataagcgg aagatacagc atggtagaac
    17101 tttctgaaag atactatggg tcggttaatc ctgaaataga aataattgat gttgttagag
    17161 aaaaaaaaat tagcagaatg aaaggacctt tctccaaaaa aatgatcgca gctattaaag
    17221 acacacttga agaaggtaga caggtcatgg tattcaggaa cagaaggtca tactcaccaa
    17281 tggttc
//
LOCUS       DAOTQL010000021        17166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3234_length_17166_cov_5.313892, whole genome shotgun sequence.
ACCESSION   DAOTQL010000021 DAOTQL010000000
VERSION     DAOTQL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_17166_cov_5.313892"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="P5293_02525"
     CDS             <1..878
                     /locus_tag="P5293_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRR48814.1"
                     /translation="FTIYDTADSRSALRGCIKELSLDEKVYKPNEVLSRISVAKNNLI
                     TPAAYMANATLIQNDVASRRPKLGEVYSLYHKKCKQAGAMDFDDILLYTNLLFRDFPE
                     ELERLRSRFSFILVDEYQDTNYAQYLIIKKLAADHHNICVVGDDAQSIYSFRGARIEN
                     ILNFKKDYPDAREFRLEQNYRSTRTIVKAANSLIAKNSNQLEKRCFSSLAEGEKIKVV
                     SAFTDQEEAFMVASSILSRIYQEKASYGDFAVLYRTNAQSRAVEEALRKRSLPYKIYG
                     GHSFYERAEVKDMIS"
     gene            889..1935
                     /locus_tag="P5293_02530"
                     /pseudo
     CDS             889..1935
                     /locus_tag="P5293_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(2082..5330)
                     /locus_tag="P5293_02535"
     CDS             complement(2082..5330)
                     /locus_tag="P5293_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR48815.1"
                     /translation="MYKITEHPILPIPEEDRVEFTFEGQKVTGQRGFTIAAALHQAGL
                     VVHKHSHEDRERTLECGIGKCGACEMLVDGKVRRICITKVDGVNNVCRINDSYMPEEL
                     KEPEGIKPAVKIYRTTVAIIGAGPAGLAVREELNKAGIANLVIDNNSKIGGQFVMQTH
                     QFFFFEKEKRFGGMRGFDIASNLAGDDHSGIILDSVIWDILEEKRLIAKNITTQEIFY
                     IDAEQIVIATGAVPFMPPFKNDDLPGVYTAAVVQRMMNRELTLLGKKILTVGAGNIGY
                     LTSYQAMQAGAKVKAIIEAMPVEGGFPVQANRVRRLGIPILTSHVLLEAIPNSDNTGI
                     TGAIIAKCENFKPIPGTELLIEDIDCINICTGLIPDNQLFRKGTEVFGRGCHGVGDSV
                     RIGEGTSAVLRGRQCAFEIMQDLGIKYNYDNYLAVSKEYIDSQQHPVQILEEPFQPSK
                     ERMSTKGFVQIDCLYGFACNPCSFACKYGAITKSSTSTVPKIDYDKCTGCMQCVHLCP
                     GLAIFGYQLNKNQIFLPIEYKADEGAEVFLVNNHGEKVGEGKIEKILKNENKTNVARV
                     KATTLEGEELLKVRGFIIKENYPEPLRLSKLDKPHDAKSYICHCEDVTVDKLLEVIGN
                     RKVISSDELKHISRIGMGACRGNRCVPRAKTLLRTYGIEVLGEPTPRGPMSNLVNLGE
                     IVNREKKETVILPEQTSGKEKGEVKKVPVLIAGGGIAGSSLFRYFSEAGYKPYLVNDG
                     LGSSWRNIAGGRPAFSLPALADIAKHNLEIFKALNDKVKIDFKLTKYVNLAHDEETYK
                     SLEMSRAWSDAYMVERKDFKKEVSPYFNENLNTYSHALITNDCWQATPGLTINAIRSL
                     GQAHGGTILENTKLIDVQKVGKEYHVTLQTYDRNYLEFRTELFINALGGDADKFAKKL
                     GIETGLYPVKHQAFITKRLPMLGKNGANLDMIIDRRKYKGFSAVYGQQLAETGQIIGC
                     ASPATDARETAKNLKINTQDFLEIVTEVFTDWIPRLSGVGFQAVWAGYYTEPRYIIDP
                     EVGLFIGMRGHGFMLSQYLAKLYVDTLLGRPVPEYFHELSLRGKGLSEDSFK"
     gene            5479..6594
                     /locus_tag="P5293_02540"
     CDS             5479..6594
                     /locus_tag="P5293_02540"
                     /inference="COORDINATES: protein motif:HMM:NF026250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HRR48816.1"
                     /translation="MRGNFIVFKVLGFIAATLFFIYFGTFLTSCKGLEFEDETTGELK
                     LAFAKSVETKSVVRDFPDSNKFILTIKSSSGSTIYNGLYGNRPAKIELGAGSYEIAVV
                     SRNFIMPEFEAPCYSDCKTVVVKAGETCVVLLTAIQSNAGLRLTFTQDFVGKFSGYTA
                     ELSDIKGKIGYPFTESRFAYMNPGIVTVRLLKFPQSGTGGKIDTITVVKREMAARDML
                     TLNLHATPSDTSSAKSGIVIDTSSRWLFEQIVIGSGGDGLSKDRAMTVDQLVSNIGAK
                     GIWVTGYIVGGDLTSTAIKFEVPFTKDTNLAIAASPTERERDRCISVCIPNNDIRPIL
                     NLCTNPSNLGKKVYIKGTVVDSYMGMVGLNPITEVQF"
     gene            6652..7236
                     /locus_tag="P5293_02545"
     CDS             6652..7236
                     /locus_tag="P5293_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HRR48817.1"
                     /translation="MEDIGFFAHETAVIDTGAIIGKGTKIWHFTHIMSKAVIGENCNI
                     GQNVVVSPEVILGNNVKVQNNVSLYTGVVCEDDVFLGPSCVFTNISNPRSAFSRRNKY
                     EKTRVGKGATVGANATIVCGNDIGEYAFIGAGAVVTDKVKPFALVVGNPARQIGWMSR
                     AGHRLFFGDESIAECPETGERYYLSMGEVSLLNL"
     gene            7246..10140
                     /locus_tag="P5293_02550"
     CDS             7246..10140
                     /locus_tag="P5293_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRR48818.1"
                     /translation="MEIKNDLLHRVMYATDASAYREMPVGVFYPRSREELVEIVNYAR
                     SHNTFIIPRTAGTSIAGQVVGSGIVVDISRYMNKILEINADERWARVEPGVVLDELNR
                     EAGKSGLFFGPETSTSNRCCLGGMVGNNSCGSHSLVYGSTRDHLIEAEVILSDGTCTH
                     FRNLSRGEFEAKLNSESKTESAIYNFVDSLLHNSRAKLEIKENFPDASLRRRNTGYAL
                     DELLATNKSEDEYINLLPLLAGSEGTLALISEIKVNLVPLPPVEKALVCAHCASLEDI
                     FKANLVALEHSPRAVEMMDNNILELGRHNLSQQKNLFFVNGNPAAILIVELAEENREF
                     LELKVNAVEADLLHSGYSYFCSKVYGSDISRVWDLRKAGLGLLTSMPGDAKPVSVIED
                     TAVAPEWLPDYIRDFREMLDRFGLSCVFHAHIGTGELHLRPILNLKKAEDVELFRTVA
                     METALLVKKYHGSLSGEHGDGRLRGEFIEVMYGSYLYNLMKSLKKTFDPQAVFNKGKI
                     VDTPLMNTYLRYEVGIESPDYKTYFDFSAQGGWLRAIEQCNGSGDCRKGIVFGGTMCP
                     SFRATGEEKDVTRGRANILRELLTRPRSKKVFNQKEILEALDLCLSCKACKSECPSNV
                     DMSRYKAEFLQHHFDSVLFPPFRTVMVANMAAVERFGSFFPKLYNFFATNHFTSSLIK
                     EIVGFTQKRELPRIMENSVEKYIKQSKMPGKGKKVYLFLDEFTKYNDGATGIAFVKLM
                     IALGYDVVVPEHFESGRAALSKGMIKYAREFARKNVGLLKDIVSSDAPLVGIEPSAIL
                     SFRDEYPDLVGEDLKAEALALAQNTFLYDEFIMREVDAGRISSESFTDKSLNIKLHGH
                     CHQKAIVTTEFSRKMLSLPVNYKVEVLSTGCCGMAGSFGYEKEHYDLSMRIGEQSLFP
                     AVRSAGDDVAIAAPGFSCRQQIKDGTGKIALHPVEILLRALKSWNIYCKN"
     gene            10165..12690
                     /locus_tag="P5293_02555"
     CDS             10165..12690
                     /locus_tag="P5293_02555"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRR48819.1"
                     /translation="MIGGRIGRYIVLVFLFFAGVGVITVAGQQTASKMLVSGVVVDAK
                     SGIPLDLAYVIIEGTSQGATTDHNGYFSIITDRRNISLKISRLGYSDANIKVLNNKSE
                     NLRIELVPVDIELNTVVVKANKVKYKNKNNPAVSLIDSVVAYRDKNRVESFDFLQYEK
                     YEKIQFALSNVSEDFKKSPVIKDISFIFENSDTTKQAGKEVLPMYIKETLYDYYYRRT
                     PRAENVVVKADKMANFEGYLDNNGITAYLNHLYQDIDIYSTNIFFLTNQFLSPIANSA
                     PLFYRYYIQDTLDIDGTKCIKLFFSPRNKTDMLFQGFMYITMDGSYAVKRIDMGVNKN
                     INLNWVKDVNIAQEFMRTADQGWVKTLDELSVDFGLNQRGMGIYGQKSVSYRNFVINL
                     PIDDSKFKKILPVADSINKSDEFWQMNRHIQLSKAEEATYSNLDSIQKVPSFRTMMDI
                     MRLAFAGFHDFGKFEVGPVGTFVSYNPIEGPRVKFGGRTTPKLFNKLIFDGYLAYGFK
                     DEKFKYNVGAVYSLTRNTVYDFPVKSLRVNYQVDTKVPGQELYYVQEGNALLSFKRGV
                     DDKRFYNKTFKAEFLDEFENHFSYSLGYNYTRQSPGGVLDFKPLDISELSLRLRYAPH
                     EQFYQGKLFREIVPSKYPIITFDYINSSKFLGGDYNYNSVKLNFFKMFYPGILGYTAV
                     RIEGGAVFGEVPYPLLQIHNANQTYYYQSIAYNLMNFLEFVSDRYASINIDHCFNGFI
                     FNKIPIIKNLRLREVITLKVLYGGLGKDNNPDFNSSLMKFPVNKEGVATTFPLGNEPY
                     VEVGCAVANIFNFVRIDFIQRVTYLNHPNVSRSGVRIMFKFDI"
     gene            12741..13055
                     /locus_tag="P5293_02560"
     CDS             12741..13055
                     /locus_tag="P5293_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48820.1"
                     /translation="MNADIIAYLRTFIDCSFEQFSDWRSVNKRTSQDAEYIEHTPVVM
                     VVMFGDSNKAVSGYGTVNLYSDCVFRVTPKGFDIQMLLDPFKKTTPPANAFCKALQYL
                     LR"
     gene            complement(12994..13578)
                     /locus_tag="P5293_02565"
                     /pseudo
     CDS             complement(12994..13578)
                     /locus_tag="P5293_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            13830..14555
                     /locus_tag="P5293_02570"
     CDS             13830..14555
                     /locus_tag="P5293_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48821.1"
                     /translation="MRIKLDIEGLKAQFADKPIIQVSDIYSYYNKREPEIKQTTIYLR
                     INTLVNDGVLQRIGRGQYHLGKNEVFIPEVSRKMKEIEKIISSKFLLTKHCQWDLSCV
                     NHFSQHLINFNVHFVDVERDVLEPMYHALKEKHPKVMLVSNLYDDISEFKNTIIVRPL
                     ISEAPIQKSGKTDVATLEKILVDFATDNEFITFQGNEIYTIFRTALQKYTLNRNTMLR
                     YASRKHKREEIEQLLKTIKTAIN"
     gene            14580..15653
                     /locus_tag="P5293_02575"
     CDS             14580..15653
                     /locus_tag="P5293_02575"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRR48822.1"
                     /translation="MIKIESSSKEWIEAVSKSKKADKILVEKVVRALTLLEGLATSDL
                     EFIFKGGTALMLLLDSSRRLSIDIDIILPDKDKDLDNILEQVCKDYGFTHYKRQVRRE
                     NSKIDKDHYMLFFESVVENKESYVLLDVLREDIHYNNIIEVPISGAFIDVSGETVSVK
                     VPDFNNILGDKLTAFAPNTTGIPYRKKDKEMGMEIIKQLYDIACICDHADNPVSISEV
                     FSTFAKTEITYREDKCTVSDVIEDIIDNALEICLRGDYGKANYDILLRGITQVRSFIF
                     SEPFHLEKAITCAAKAAYIVSLIKYEKDKIERYHPRILPELIDWQIKEPMNTKLNKIK
                     KSDREAFFYLYKIWEMQHDSLSK"
     gene            15721..16041
                     /locus_tag="P5293_02580"
     CDS             15721..16041
                     /locus_tag="P5293_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48823.1"
                     /translation="MIDAKNQIGVYDCDLESLEMLKPEMPKMWINYREYFIQEIDYIS
                     KTFWESFVASQKSFIGMKLDETVSGTLLLPLERAAICYYIDMPNNNYKLLEVRFDNQL
                     VIGK"
     gene            complement(16357..16430)
                     /locus_tag="P5293_02585"
     tRNA            complement(16357..16430)
                     /locus_tag="P5293_02585"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16394..16396),aa:Asp,seq:gtc)
     gene            complement(16489..17061)
                     /locus_tag="P5293_02590"
     CDS             complement(16489..17061)
                     /locus_tag="P5293_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRR48824.1"
                     /translation="MRIIGGEQRGRQIVPPSNFKARPTTDFAKEALFDILSNKYDFSK
                     ISFLDLFSGTGSISYEFASRGCRDITAVEMNPVHAAFIKSSASKFFGSESYVIHVVHH
                     NVFDFLKICKKQFDIIFADPPYDISELSEIPEKILNTHIFNEHALLIFEHSGRYNYEN
                     NPHFIEERKYGNVHFTFFSAEIQKFNKQNL"
BASE COUNT         4970 a         2877 c         3792 g         5527 t
ORIGIN      
        1 attttacgat ttatgatacg gcagactcaa ggagtgcttt gaggggatgt attaaagagc
       61 tttctctcga tgagaaagtt tataaaccaa acgaggtgct ttcgaggatt tctgttgcca
      121 aaaacaattt gataactcct gctgcttata tggccaatgc tactttgata cagaatgacg
      181 tggcttcgag aagaccaaaa cttggtgaag tttattcttt gtatcataag aagtgcaaac
      241 aggcgggggc aatggatttt gatgatattt tgctgtatac caatttgctt ttcagggatt
      301 ttcctgaaga gctcgagagg cttcgttcga ggttttcgtt tatacttgtg gatgagtatc
      361 aggacacaaa ttatgctcaa tatcttataa ttaaaaagtt ggcagcagat caccataata
      421 tatgtgtggt tggagatgat gcacagagta tttattcttt caggggtgca aggattgaga
      481 acatccttaa ttttaaaaaa gattatcctg atgccagaga atttcgcctc gaacaaaatt
      541 accgttctac aagaactatt gttaaggcag ccaacagtct tattgccaag aattcaaacc
      601 agcttgaaaa gaggtgtttt tcctctttgg ctgagggaga aaagataaag gttgtaagtg
      661 cgtttaccga tcaggaagag gcatttatgg ttgcatcttc tattttatca cgtatttatc
      721 aggaaaaggc ttcttatggt gactttgcag ttctatacag gacaaatgct cagtcgaggg
      781 ctgtagagga ggctttgaga aaacgttctc ttccatataa aatctatgga gggcattctt
      841 tttatgagag agcagaggtt aaggatatga tttcttaaca agaaggatga tgaggctttc
      901 agaagaatcg tgaatgtgcc tccgagaggc attggcgata cgactcttgc ctgtcttact
      961 gcggctgcaa atgctgaggg tatttcattg tgggatgcca tgacgagtgg ccagatgagt
     1021 gttatgggta tcaaacctgc tgctgtcaac aagcttttgc tttttgcaag tatgatttcg
     1081 gagattagtg agcatattta ctgttacaat gcttacgatg ttgctatgga agtgcttgtc
     1141 aagtcgggat atctggatta tcttaaaagt gacatttccg tggagggaca ggctcgtctt
     1201 gacaatgtgg aagagttgat gaatagtatt aaggcttttg ttgaagagtt cgaggaggca
     1261 tctacggacg gtgatgtaga tgtagaagag ggaaagattg ctcctgcggt ctctttgacc
     1321 aattatgtcg aaaacatatc tcttttgagt tctgttgacg agaataatgg tcaacaagac
     1381 tcaaataaaa taagcctgat gacagttcat tcggcaaagg gacttgaatt tccttatgta
     1441 tatgtaatag gcatggagga aaatctgttt ccttcgggta gcatgtcggg aattaatttc
     1501 aacgatatgg aggaggagag aaggttgttt tatgttgcaa ttaccagggc caaatattca
     1561 gtgacacttt cttatgccca gtcgcgtttc aggtggggtt ctcatgttaa ttatcctgta
     1621 agcagatttt tgagggaaat tgatttggaa tttttagatt ggccggaaaa tccaatacag
     1681 acgattaatc ctttggaaga gatgcgcaat atgcataaca gacagttata ccataaacct
     1741 gttgaccctg attttaaggc agattctcca tcaaagttga gagtaggtca aaaggttgaa
     1801 catgacaggt ttggttatgg tattttactg gagttggaag gtgaccagcc gaatgctaag
     1861 gcgattgttg attttgaaca gggcggacgc aagactcttc ttcttaaatt tgccaaactc
     1921 cgtgtagtag agtaaatctt tattatttta cttttaattt ataatctggc acagagtttg
     1981 tataatattt agatgaagtt tttcatagtt taagtttagg ttaagtaatg gggcgttgtt
     2041 ccaacaggaa caatgcccca tgaaatttta tagtggtttt tttatttgaa agagtcttca
     2101 cttaaacctt tccctcttaa gctgagttca tggaagtact caggaacagg acgtccaagc
     2161 agtgtgtcaa catataattt tgcaagatat tgcgaaagca tgaaaccatg tcctctcatt
     2221 ccaatgaaca atccgacttc tgggtctatg atatagcgcg gttctgtgta atatcctgcc
     2281 cagactgcct ggaagccgac tcctgaaagt ctgggaatcc agtctgtgaa aacttccgtt
     2341 actatttcaa gaaaatcctg tgtgttgatt ttgaggtttt ttgcggtttc cctggcatca
     2401 gttgcagggg aggcgcagcc aataatctgg cctgtttcgg caagttgttg tccatataca
     2461 gctgagaaac ccttatattt tcttctatct atgatcatat cgaggtttgc tccgtttttt
     2521 cccagcattg gcagtctttt ggttataaag gcctggtgtt ttacggggta gagtccggtc
     2581 tctataccga gtttttttgc aaacttgtcg gcatcgcctc cgagggcatt tatgaaaagt
     2641 tctgttctga attcaagata attcctgtca taagtttgaa gtgtaacatg gtactctttg
     2701 ccaactttct gcacatctat cagtttggtg ttctcaagga ttgtacctcc atgtgcttgc
     2761 cccaggcttc tgattgcatt tatagtaagt ccgggagtgg cctgccagca atcgttggtt
     2821 ataagtgcat gactataggt gttgaggttt tcgttgaagt atggagagac ttcctttttg
     2881 aaatctttgc gttcaaccat ataagcgtct gaccatgctc ttgacatttc gagcgacttg
     2941 tatgtctctt cgtcatgagc aaggttaaca tatttggtga gtttgaagtc aattttgact
     3001 ttatcgttta atgctttgaa tatctctaaa ttgtgtttgg ctatatctgc gagagcaggg
     3061 agtgagaatg ccggacgtcc tccggctatg tttctccagg agcttccaag tccgtcattg
     3121 acaaggtaag gtttatagcc agcttcagaa aaatatctga aaagtgatga accggctatg
     3181 cctcctccgg caataaggac gggaactttc ttaacttccc ctttttcctt acctgacgtt
     3241 tgttcaggaa ggataaccgt ttcttttttc tccctgttta ctatctctcc aagattgacc
     3301 agattggaca ttgggcctct tggtgtcggt tcgccaagca cttcgatgcc atatgttctg
     3361 agaagagttt tggctcttgg aacacatctg ttgcctcggc aggctcccat accaattcta
     3421 gagatatgct taagttcgtc cgatgagata actttacggt ttcctatgac ttcgagtagt
     3481 ttgtcaactg tgacatcttc gcagtgacat atatatgatt ttgcatcatg gggtttgtcg
     3541 agttttgaaa gcctgagcgg ttcaggataa ttttccttaa tgatgaagcc tctgactttc
     3601 agtaattctt ctccttctag tgttgttgcc ttgactctcg caacatttgt cttgttttca
     3661 ttcttaagga ttttttcaat tttcccctct ccgacttttt ctccatggtt gtttacgaga
     3721 aatacttcag ctccttcatc agctttgtat tctataggaa ggaatatctg atttttgttt
     3781 aattgatatc caaaaattgc aagaccaggg cacaggtgaa cacactgcat acagccggta
     3841 cacttgtcat aatctatttt tggaacagta gaggtggagg atttggtaat tgctccatat
     3901 ttacaggcga aagagcaagg gttgcatgca aatccataga gacagtctat ctgtacaaag
     3961 ccttttgtac tcatcctctc tttggatggt tggaaaggtt cttcaagaat ttgtactgga
     4021 tgttgctgtg aatcgatata ttctttggag acagcgagat agttatcgta attatatttt
     4081 atcccaaggt cctgcatgat ttcaaatgca cattgacgac cccttagaac ggctgatgtc
     4141 ccttctccta ttctgacaga atctccaacg ccgtggcagc ctcttccgaa tacttctgtt
     4201 ccttttctga ataattggtt gtcaggaata agaccggtac aaatatttat gcagtcgata
     4261 tcttcaatga gcaactctgt tccgggaatt ggtttgaaat tttcacattt ggcaattatg
     4321 gctcctgtga tgccggtgtt gtcggagttt ggaatagctt cgagcagtac gtgtgaggta
     4381 agaataggaa taccgagtct ccttactctg tttgcctgaa cagggaaacc tccttcaaca
     4441 ggcattgctt caataattgc cttgactttt gctccggctt gcattgcctg atatgaggtg
     4501 agatatccta tgtttcctgc acctacagtg agtatctttt taccgagaag agtgagctcg
     4561 cggttcatca ttctctgaac aacggcagca gtgtatactc cgggaagatc atcgtttttg
     4621 aatggcggca tgaatggaac agcaccggtt gctattacaa tctgttctgc atctatgtag
     4681 aatatttcct gggtggttat attttttgca atcagtcttt tttcttctaa aatatcccaa
     4741 atcacagagt cgagaattat tcctgaatga tcatctcctg caagattgga tgcgatgtca
     4801 aaacctctca ttccgccaaa tcttttctct ttttcgaaaa agaaaaactg gtgggtttgc
     4861 attacaaact gacctccaat tttactgttg ttatcaatga caaggtttgc aatgcctgct
     4921 ttgttaagtt cttctcttac tgcaagcccg gccggtccgg ctccgataat ggcaactgtt
     4981 gtacgataaa tcttgactgc aggcttaatt ccttcaggtt cttttagctc ttcaggcata
     5041 taggagtcat tgattctgca tacattatta actccgtcta ctttggtgat acatatacgg
     5101 cgaactttcc cgtcaactag catttcgcag gcgccacatt taccaatgcc gcattcgaga
     5161 gtgcgctcac gatcttcatg actgtgtttg tgtactacta atcctgcttg atgtaaagcg
     5221 gctgcaatag taaatcccct ttgacctgta actttttgtc cttcgaatgt aaattctact
     5281 ctatcttcct caggaatagg aagaatagga tgttcggtga ttttatacat agttaatttt
     5341 taattttaac aaaattagta aaaatcatat cgaagaattt taaaatcaga taaattttaa
     5401 aaaatattga tgattttgtt acagatttat aacaatttat aatagttttg gctcattaat
     5461 caattaaatc tgtgaggtat gaggggtaat tttattgttt ttaaagtgtt gggatttatt
     5521 gcagcaacgc tgtttttcat ctattttgga acttttctga cttcttgcaa aggtcttgaa
     5581 tttgaagatg aaacaaccgg agagttgaag cttgcttttg caaaaagtgt tgaaacgaaa
     5641 tcggtagtca gggattttcc tgacagtaac aaatttattc ttactatcaa gagtagttcc
     5701 ggttcaacca tttacaatgg tttatatggg aacaggcctg caaaaattga gctcggcgca
     5761 gggagttatg aaattgcggt agtttcaaga aactttatca tgccagaatt tgaagcgcca
     5821 tgttattctg attgtaaaac agtagtggtc aaagcgggtg aaacttgtgt ggtactatta
     5881 acggcgattc agtcaaatgc tggtttgagg ttgacattta cgcaggattt tgtcgggaaa
     5941 ttttccggct atactgcaga attgtctgac attaagggta agataggata tccatttact
     6001 gagagcaggt ttgcctatat gaatcccggt atagtgacgg tgagactttt aaaatttcct
     6061 caatcaggca ctggtggtaa gattgataca ataactgttg ttaaaaggga aatggctgca
     6121 agggacatgc ttactcttaa tttgcatgca accccttctg acacctcttc tgccaagtcc
     6181 ggtatagtaa tagatacttc atcaaggtgg ctttttgagc agattgttat cggaagtggt
     6241 ggtgatggtc tttcaaaaga tagagctatg acagtagatc agcttgtttc aaatattggc
     6301 gcaaaaggga tatgggttac aggttatatt gtaggcggag atcttacctc cacagctatc
     6361 aaatttgaag ttccatttac caaggatacc aatcttgcaa ttgctgcttc tccaactgag
     6421 agggaaagag atagatgtat ttctgtatgt attcctaata acgatatcag accaatttta
     6481 aatttatgca ccaatcctag caatctgggt aagaaagtct atattaaggg tactgtagta
     6541 gattcatata tggggatggt cgggcttaat ccgataacag aggtacagtt ttaaaaaata
     6601 gggatattgt tgtgaattgc tatatttgca cctgtttaaa agatcatagc catggaggat
     6661 attggatttt ttgctcacga gactgcagta atcgataccg gtgccattat tggcaaaggg
     6721 actaaaatat ggcattttac acatattatg agcaaagctg ttattggaga aaactgcaat
     6781 attggtcaaa atgtggttgt ctctcccgag gtgattcttg gcaataatgt taaggtgcaa
     6841 aataatgtct ctctctacac aggggttgtt tgtgaagatg atgtttttct tggtccgtca
     6901 tgtgttttta caaatatatc caatcctcgc agtgcctttt cgcgtaggaa caagtatgag
     6961 aaaactcgcg tgggaaaggg ggcaactgtt ggggcaaatg caactattgt atgtggaaat
     7021 gatattggcg aatatgcctt tatcggggca ggagcagttg tgacagataa agtcaagcct
     7081 tttgcacttg tggtaggaaa tcctgcgaga cagattggtt ggatgagtcg tgccgggcac
     7141 aggttgtttt ttggggatga aagtattgct gagtgtcctg aaacagggga gaggtattat
     7201 ttgtctatgg gtgaggttag tttgctcaac ctttagtgaa ttaagatgga aattaagaat
     7261 gatctgttgc acagagtaat gtatgcaact gatgcctctg cctacagaga aatgcctgtt
     7321 ggtgtctttt atcctcgcag cagggaagaa cttgttgaga ttgtaaatta tgcgcgttct
     7381 cataatactt ttattattcc gcgtactgcc ggcacttcta ttgccggtca agtggttggt
     7441 agcggcattg tggtggatat ttcgagatac atgaataaga tactcgagat taatgctgat
     7501 gaaagatggg ccagggttga accgggagtt gtgcttgatg agttgaacag ggaggcagga
     7561 aaatcaggcc tttttttcgg gcctgagact tctacttcca atcgttgctg tctgggtgga
     7621 atggttggta acaattcctg tggctcacat tcacttgttt atgggagtac cagagatcat
     7681 ttgattgagg ctgaagttat tctgtctgat ggtacttgca ctcactttag aaatctgtca
     7741 aggggggagt ttgaggcaaa acttaactct gaaagtaaaa cagagtcggc tatttataat
     7801 tttgtagatt ctcttcttca taacagcaga gctaagttgg agattaagga aaattttcct
     7861 gatgcttcac tcagaaggag aaatacggga tatgctctcg atgaactttt ggctacaaac
     7921 aaaagtgagg atgagtatat aaatctttta ccacttttgg caggatctga ggggacgctt
     7981 gctttaattt cggaaataaa agttaatctt gtgcctcttc ctccagtaga gaaggctctt
     8041 gtctgcgctc actgtgcttc gcttgaggat atttttaaag ccaatcttgt agctcttgag
     8101 cattcacctc gggctgtaga gatgatggat aataatattc ttgaattggg caggcacaat
     8161 ctttcacaac aaaaaaatct gttttttgtg aatggcaatc ctgctgcgat tttaattgtt
     8221 gagcttgctg aagagaacag ggagtttctg gagcttaagg tgaatgctgt ggaggcagat
     8281 ttgttgcatt ctggatatag ttatttttgc tcaaaggttt acggatctga tatatctcgt
     8341 gtatgggatt tgagaaaggc cggcctggga cttctaactt ctatgcctgg tgacgcaaag
     8401 cctgtgtcgg ttattgagga tacggctgtt gcacctgagt ggctgcctga ttatataagg
     8461 gattttagag agatgcttga ccgttttggg ctttcatgtg ttttccatgc tcatatagga
     8521 acaggagaac ttcaccttag accaatcctc aatcttaaaa aggctgaaga tgtggagcta
     8581 ttcagaacag ttgcaatgga gactgctttg cttgttaaga aatatcatgg ctctctttct
     8641 ggagagcatg gagacggaag attgagaggt gagtttattg aggtaatgta tggctcttat
     8701 ctgtacaatc tgatgaagag tcttaaaaaa acttttgatc ctcaagctgt ttttaataag
     8761 gggaaaattg tggatactcc tctgatgaac acttatctta gatatgaggt tggaatagaa
     8821 tctccggact acaagactta ttttgatttt tctgcgcagg gaggatggtt aagggcaatc
     8881 gaacagtgta atggttctgg agactgtcgt aagggaattg tatttggcgg aaccatgtgt
     8941 ccgagtttca gggctaccgg cgaggagaaa gatgttacaa gagggagggc taatatttta
     9001 agagagcttt tgactcgtcc cagaagtaaa aaggttttta accagaagga gattcttgaa
     9061 gcgttagacc tctgtttgtc atgcaaggct tgcaagagtg aatgtccttc gaatgtggat
     9121 atgagcagat ataaggctga gtttttgcaa catcattttg attctgtatt atttcctccc
     9181 tttagaacag ttatggttgc aaatatggct gctgttgaga gatttggctc gtttttccca
     9241 aaactttaca atttttttgc aactaatcat tttacttctt ctcttattaa agaaatagtg
     9301 gggtttactc aaaaaaggga actgcctcgt attatggaga attctgtgga gaaatatata
     9361 aagcaatcaa aaatgccagg gaaggggaaa aaagtctatt tatttctcga tgaatttaca
     9421 aaatataatg atggtgctac aggcattgca tttgtcaaat tgatgattgc tcttggttat
     9481 gatgttgtgg tgcccgaaca ttttgaaagc ggcagggctg ccttgtcaaa ggggatgatt
     9541 aagtatgcca gagagttcgc tagaaaaaat gttggtctgt taaaagatat agtaagtagt
     9601 gatgctcctc ttgtagggat tgaaccttct gcaattcttt ctttcaggga tgaatatcct
     9661 gatttggtgg gggaagactt aaaagctgag gcgctggctc ttgctcaaaa tacatttctt
     9721 tatgatgagt ttattatgag ggaggtggat gccggaagga tctcttcgga aagttttacg
     9781 gataagtctc ttaatattaa acttcatggc cattgccatc aaaaggcaat tgttactaca
     9841 gaattttcaa gaaaaatgct ctctttgcca gtaaattaca aagtggaggt tctttctacc
     9901 ggctgttgtg gaatggcggg ttcttttgga tatgaaaagg aacattacga tctttcgatg
     9961 aggatagggg agcaatctct ttttcctgca gtcaggtctg ccggtgatga tgtggcaatt
    10021 gctgcacctg ggttctcctg caggcagcag ataaaggacg gaacaggcaa aatagctctt
    10081 catcctgtag aaatactatt aagagcactc aaatcctgga acatttattg caagaattga
    10141 cttctttgat taatgaatta atgaatgatc ggtggaagaa taggtagata tatagttttg
    10201 gtttttcttt tttttgccgg tgttggagta ataactgttg ccggacaaca gactgcatca
    10261 aaaatgttgg tgagtggtgt ggtggttgat gctaaaagcg ggattccgtt ggatcttgca
    10321 tatgtgatta ttgaaggcac ttctcaaggt gctactactg atcacaacgg ttacttttct
    10381 attataactg ataggagaaa tattagtttg aaaatatcaa gactggggta tagtgatgca
    10441 aatattaagg tactgaataa caaatcagaa aatttaagaa tagaactagt gcctgttgat
    10501 attgaactta atacagtggt ggtaaaagca aacaaagtca agtacaaaaa caagaataat
    10561 cctgcggttt cgctcattga cagcgtagtg gcttacaggg acaaaaatcg tgttgaaagt
    10621 tttgattttc ttcaatatga gaagtatgaa aagattcagt ttgctctgag taatgtttct
    10681 gaagatttta agaagagtcc tgtgataaag gatataagtt ttatttttga aaattcagat
    10741 actactaaac aagctggtaa ggaagttctg ccaatgtaca ttaaggagac tttgtatgat
    10801 tattattaca gaagaactcc gagagctgaa aatgtggttg ttaaggcaga taaaatggca
    10861 aattttgagg gataccttga caataatggg atcactgcct atttgaatca tctttatcaa
    10921 gatatagaca tttacagtac taatatattt tttcttacaa accagttttt aagtcctata
    10981 gcaaattctg ctcctctatt ttacagatac tatattcaag atactttaga tatagacgga
    11041 accaaatgca tcaaactgtt tttctctccg agaaataaga ccgatatgct ttttcagggt
    11101 tttatgtata tcactatgga tggatcgtat gctgtaaaga ggatagatat gggtgtgaat
    11161 aaaaatataa atcttaactg ggttaaggat gtgaatattg cacaggagtt tatgaggacg
    11221 gctgatcagg gctgggtaaa gactcttgat gaactttcgg ttgattttgg attgaatcag
    11281 cgagggatgg gcatttatgg gcaaaagagt gtatcctaca gaaattttgt tattaattta
    11341 cctattgatg attcgaaatt caaaaagatt ttgcccgtgg ctgacagtat aaacaaaagt
    11401 gatgaatttt ggcagatgaa cagacacatt cagctttcca aggctgaaga ggctacttac
    11461 tccaatctgg acagtataca aaaagtgcca tcttttagaa ctatgatgga tattatgagg
    11521 cttgcatttg cgggatttca tgactttggc aagtttgaag ttggtcctgt aggtacgttt
    11581 gtaagttaca atcctattga gggtccaagg gttaaatttg gtggaagaac aactccgaag
    11641 ttgttcaata agttgatttt tgatggatat cttgcgtatg gttttaagga tgagaagttc
    11701 aaatataatg taggtgcagt gtattctctt acaagaaata ctgtttatga ttttccagta
    11761 aagagcttga gggttaatta tcaggttgat actaaagttc cgggacagga gctttattat
    11821 gttcaggagg ggaatgctct tctttccttt aaaagaggtg ttgacgacaa acgtttttac
    11881 aataaaactt ttaaggctga atttttagat gagtttgaaa atcatttttc atattccttg
    11941 ggttataact atacaagaca gtctcctggc ggtgttcttg atttcaagcc cttagatatt
    12001 tcagaattaa gtttgagact cagatatgca ccacatgaac aattttacca ggggaaactt
    12061 ttcagggaaa ttgtaccgag caaatatccg atcatcacat ttgactatat aaacagttcc
    12121 aaattcttag gtggagacta taattataat tcagtcaagt taaatttctt taaaatgttt
    12181 tatcccggaa ttcttggtta tactgctgtc agaattgaag gaggggccgt attcggagaa
    12241 gtgccttatc ctttgcttca aattcataat gcaaaccaga catattacta tcagtcaatt
    12301 gcctataact tgatgaactt tcttgaattt gtctcagatc gttatgcttc aataaatatt
    12361 gatcactgtt tcaacggctt tatatttaac aagattccga tcataaagaa tcttcgtcta
    12421 agagaggtta ttacactcaa agttctttat ggagggttgg gcaaagataa taatcctgac
    12481 tttaactctt ctcttatgaa atttccggta aataaggaag gtgttgccac aactttccct
    12541 cttggcaatg aaccatatgt agaggtaggc tgtgcggtgg caaatatttt taatttcgtt
    12601 aggatagact ttattcagcg tgtgacatac ctcaaccacc ctaatgtgtc aagaagtggt
    12661 gtgcgaataa tgtttaaatt tgacatttga ttttattcaa caagaaattt agctagtgtc
    12721 tactaaaaat ttaagctaag ttgaatgccg acatcatcgc atatttgagg accttcatcg
    12781 actgctcttt cgaacagttc tctgattggc gttctgtcaa taagagaacc tcgcaagatg
    12841 cggagtacat cgaacatact cctgttgtga tggtagtcat gttcggcgat agcaacaagg
    12901 cagtaagcgg atatggcaca gtaaatctgt attctgactg cgttttcaga gttaccccaa
    12961 aaggatttga tatacagatg ctgcttgatc catttaaaaa aacaactcca cctgccaacg
    13021 ctttttgtaa agcattgcaa tatcttttgc gctgatataa aatgcattgg taatatatat
    13081 aaacgtgcgc ttgagttctt tggagtatta tacaattctg cgaagtcttc caaggtattt
    13141 ctttttggat tgatacccgg taagttcgat agtctgatcg cggataaccc cgtcaacacc
    13201 ctcgacaagg tcttcaccat caatgatctt atactgaagc ctgcctttct gacggatgat
    13261 aaagtaagaa ccaatgatat tgattttata caatctgtcc agatcaaagt accctctgtc
    13321 gaagatgtag taggcattgg gttcgtaggg gatatcatcc atcgcctgag tatcgtgaac
    13381 attggcattg gtgatattga tgtatgccgg aatctgcgta acgacatcaa aaagagtatg
    13441 agccttgatc ccactttttg tactgcggaa cttagcccac cagaaaaggc tgaggcagag
    13501 gtcaattgtc gtagaatcaa aggcttcctt tggcagaagc ttgataagtt gtgtaaatac
    13561 ataagatcca gtgttcatat tgctttaagg attagcctgg gggcaatata cgaaatcaaa
    13621 tgtagatgtt ggttttgtcg ctgtatttta ttaaatatta aataattata aaggttaaac
    13681 aaaaagttta gtagacacta gtgatttttg gtgattaaac agaaagcccg taccgtcgtg
    13741 atgacaggca cgggctttat tataaattcg gcaataaatg ctggttattg ccgaatttat
    13801 aatatctttg ttcccataaa aagatgaaga tgagaataaa attagatata gaaggactta
    13861 aagcacagtt tgctgacaaa cctatcatac aggtttcaga catctattca tattacaata
    13921 agagagagcc tgaaattaag caaacgacca tatacctgag aattaacacg cttgtaaatg
    13981 acggtgtgct tcaacgaata ggcaggggac aataccattt gggtaaaaat gaagttttta
    14041 tacctgaagt aagtcgcaaa atgaaagaaa ttgaaaagat tatcagtagt aagtttctac
    14101 ttaccaaaca ttgtcaatgg gatttgtcgt gtgtaaacca tttcagccaa cacttgatta
    14161 attttaatgt gcattttgtt gatgtggagc gcgatgtatt agagccaatg tatcatgcat
    14221 taaaagaaaa acatccaaag gttatgttgg taagtaatct atacgatgat atttccgaat
    14281 ttaaaaatac tatcattgta cgtccgctta tttccgaagc acctattcaa aagtcaggta
    14341 agactgatgt tgctactctt gaaaaaatac tggttgattt tgctacggat aacgagttta
    14401 tcacttttca gggtaatgaa atttacacca ttttccgtac agcattacaa aaatacacgc
    14461 tcaatcggaa taccatgttg cgctatgctt ctcgtaagca taaaagagaa gaaatagaac
    14521 aattattaaa aactataaaa acggcaataa actaaaaata ttgccgaatt gtcattttta
    14581 tgattaaaat agaaagttcg tcaaaagaat ggattgaagc cgtttccaag tcaaagaagg
    14641 cggataaaat tcttgtggag aaagttgtca gggcgctaac gctgttggag ggattagcaa
    14701 cttccgattt agaatttata ttcaagggtg ggactgcctt gatgttgcta ctggattcgt
    14761 ccagacggtt gtcgatagat attgacatca ttcttcccga taaggataag gatttggata
    14821 atatccttga acaagtatgt aaagactacg gatttactca ttataaaagg caagtacgga
    14881 gggaaaattc aaagattgac aaagaccatt atatgctctt tttcgaatcg gtggtagaaa
    14941 acaaggaatc ctatgtatta ctggatgtgc taagagaaga tattcattac aacaatataa
    15001 ttgaagtccc catatccggt gcattcatag atgttagtgg tgaaacggta agcgtaaaag
    15061 ttccggactt taataatata cttggcgata agctgactgc ttttgctcct aatactacgg
    15121 gtatccctta cagaaagaaa gataaggaaa tgggtatgga gattataaaa caactttatg
    15181 acatagcctg tatctgtgat catgctgaca accccgtgtc tatttcagaa gtattttcaa
    15241 cttttgctaa aacagaaata acatatcgcg aagataaatg cacggtatcc gatgttattg
    15301 aggatattat tgacaatgca ctggaaatat gcctgcgtgg ggattacggc aaggctaatt
    15361 atgatatttt attgagagga ataacccaag tcagaagttt tatcttttcc gaaccgtttc
    15421 atcttgaaaa agcaatcaca tgtgctgcaa aagcagctta tattgtgtca ttgataaaat
    15481 atgagaaaga taaaatagaa cggtatcatc caagaatttt gccggagttg atagattggc
    15541 agataaaaga accaatgaat actaagctga ataaaatcaa aaagtccgac agagaagcgt
    15601 tcttctattt atacaaaata tgggaaatgc aacatgatag cttaagtaaa taaaaacagg
    15661 tacgatatga aatttagtga acgtagcttt ccaattctac atgctttaaa attccccaat
    15721 atgattgatg ctaaaaatca gattggggta tatgattgtg atttagaaag tttggaaatg
    15781 ctaaaacccg aaatgcccaa aatgtggatt aattatagag aatacttcat tcaagagata
    15841 gactatattt caaaaacatt ttgggaatca tttgttgctt cacaaaaaag ttttatcgga
    15901 atgaagttgg acgaaacagt gtcaggcacc ttattactcc cgcttgaacg cgctgcgata
    15961 tgttactata ttgacatgcc taataataac tacaaattgc ttgaagtcag gtttgataat
    16021 cagcttgtaa tcggtaaata gaaatacgcc aaataaggtc aatcccctgc caatttcaga
    16081 caaactgtac cccctgcggc ttttttcaaa agggggtgca ctttaggtta cttgctttgt
    16141 ctttttctgg ctttttcttg tttgtcttac ttttggaacc tcttgtctta ggttcccggc
    16201 ggtacggacg ggaaagccac cttttcgggt taattgactt tgtttcaatg cgttatcccc
    16261 ttgattcttc ttagggggta ctgtttaggt taggaactcc taactaactg atttgcaaca
    16321 tttcaactac tcgtcgcttt aagcaccttt ttgttgcggt ctggacggga ctcggacccg
    16381 cgaccccatg cgtgacaggc atgtattcta accagctgaa ctaccagacc ttatttcctt
    16441 catccctttt ggggattggg attgcaaaga taggagcttt tttgttattt acaaattttg
    16501 tttgttgaat ttttgaattt ctgcagagaa gaaagtgaag tgaacgtttc catattttct
    16561 ctcctctata aaatgagggt tattttcgta attatatctg cctgaatgct caaaaatgag
    16621 gagagcatgt tcattgaaaa tgtgtgtatt cagtattttc tccgggattt ctgacaattc
    16681 tgaaatatca tatggagggt ctgcaaatat aatatcgaac tgttttttgc atatcttgag
    16741 aaaatcaaaa acattgtgat ggacaacatg aataacataa ctttctgatc caaaaaattt
    16801 tgacgcactg ctctttataa acgcggcgtg aacgggattc atttctacag ccgtaatgtc
    16861 tctgcagcct cttgaggcaa actcatagga aatgcttcct gtacctgaga agaggtctaa
    16921 aaaagagatt ttgctgaaat cgtatttgtt tgatagtata tcaaagagag cctcttttgc
    16981 gaaatctgtg gttggtctgg ctttgaagtt ggagggtggc actatttgcc tgcctctctg
    17041 ttctcctcct attattctca tagtttgaaa gtgagtaagg agagttgcat ttcactgata
    17101 tggtctttaa gagggatttc agaatttctg aagtatctaa tcttcgaaaa atattttgtc
    17161 atgttc
//
LOCUS       DAOTQL010000022        17043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3262_length_17043_cov_5.104956, whole genome shotgun sequence.
ACCESSION   DAOTQL010000022 DAOTQL010000000
VERSION     DAOTQL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_17043_cov_5.104956"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2017)
                     /locus_tag="P5293_02595"
     CDS             complement(<1..2017)
                     /locus_tag="P5293_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRR48825.1"
                     /translation="METNIINLSRKVSMVILALLVSRTDGFTSSGNFTCISTVSEVPD
                     SLDYEIYDSLKNSIGKTIVLDKIIMSASYLKETTSPMRIKSVDEHEISMKAFGRTYPE
                     LLKGIPGIYATSETGSYGDAKINIRGFKQENISVLLNGIPISGLVSGNMYWNNWLGLA
                     DATFSIQVQKGIGASMLSDNSVGGSINIITKTPSEEQKVSGGLFFSPFGADKGGQAKG
                     FVSYNSGLLPKGWGVSAMLSYTGGSGYVEATDVDSWAYIFNVSKKINSENKILLTVLG
                     SPDRHEQRSVRLSKDEVDKYGVRYSKNWGYLNGEKKNLSKNFYHKPYITLNHFYSPDS
                     KIEMTNTLYFTFGDGGGRWSESKGRRIISYQKDGHIDWDSVIEDNKAVTGTEASGSAI
                     NILSNYLAGHTHAGIRNNTSYSINDKLKIEGGVHYLYYSTWEKEKIIDLLGGEYWYED
                     YATNSLAGVAGRNPIKHVGDYIRTRNGKITNNATIYISEAYNSEKWNIRGGLSYMWNS
                     YRRWDTYNYVDDPSSLATGSKSDLVTVSGFSAKGGANYKLTKKSSIYLNAAAYSRIPY
                     SSVFFAQGTNEITSGVKNEKNFLSEAGYRYIFQRGSLELTGYWAYWKNKTIVSNPYTQ
                     QDNNDVSFMIQGLDALHYGTELTVTFAPYRWLSFEGNLSVGHWNWKND"
     regulatory      complement(2017..2132)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2291..3115)
                     /gene="tsf"
                     /locus_tag="P5293_02600"
     CDS             complement(2291..3115)
                     /gene="tsf"
                     /locus_tag="P5293_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRR48826.1"
                     /translation="MEIKAADVAKLRQMTGAGMMDCKNALVEANGDYEKAKDIIREKG
                     KLIAAKRADREISEGSVIARVNSSNNKAILICLGCETDFVSRNADFQELANKIADVAL
                     ESFPADLEALKSLKIDNMTIEEAITQQTGKSGEKHALLFYSRIEAPYVDYYIHTNGKV
                     ATIVGFSKTVSHEVGKEIAMQITAMSPVSVSRESCPKEVIDKELEIYRIQIREEGKPE
                     NMVEQIAQGKLNKFFKESTLEEQTFVKDGKITVAAYLKSVDPEVRVTEFSRFSLND"
     gene            complement(3160..4014)
                     /gene="rpsB"
                     /locus_tag="P5293_02605"
     CDS             complement(3160..4014)
                     /gene="rpsB"
                     /locus_tag="P5293_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRR48827.1"
                     /translation="MPRTTFQELLDAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTIIKIDEAAYVMKQMARAGRKILFVATKKQAKEIVAEYAKSVNMPYVTERWPGGMLT
                     NFPTIRKAVKKMNTIDKMMTDGTFETLAKREKLQITRQRAKLEKNLGSIADLNRLPSA
                     LFVVDIQKEMNAVKEANRLNIPVIAMVDTCCDPSQIDYVIPANDDAAKSISLIMGVLA
                     QAISEGLAERKFEKEKESAESGKDSGEVNVKESGRKMRPRKNAKNENEGEKPTAIEEK
                     PVDETTES"
     gene            complement(4220..4609)
                     /gene="rpsI"
                     /locus_tag="P5293_02610"
     CDS             complement(4220..4609)
                     /gene="rpsI"
                     /locus_tag="P5293_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRR48828.1"
                     /translation="MEVINALGRRKTAVARIYVNSGKGNITINGRDLDSYFRDDSLKY
                     IVRQPLELTNTLSQLDIRVNLCGGGIKGQAEALRLAIARALCKIDTEAYRPVLKSNGF
                     LTRDSREVERKKPGQPGARKRFQFSKR"
     gene            complement(4616..5068)
                     /gene="rplM"
                     /locus_tag="P5293_02615"
     CDS             complement(4616..5068)
                     /gene="rplM"
                     /locus_tag="P5293_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRR48829.1"
                     /translation="MDTLSYKTVSANKATVNKDWMVIDANGAILGRLASRVALILRGK
                     HKPNFTPHVDCGDNVIIINASKVRLTGKKLTDKVYVRHTGYPGGQRFSTPKELLARKP
                     LAVVEHAIKGMLPKNRLGAEMFRHLYVYEGPEHPHEAQQPKVINLNEI"
     gene            complement(5149..6138)
                     /gene="pfkA"
                     /locus_tag="P5293_02620"
     CDS             complement(5149..6138)
                     /gene="pfkA"
                     /locus_tag="P5293_02620"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRR48830.1"
                     /translation="MNSKRKINRIGVLTSGGDAPGMNAAIRAVVRTAIYNQLEVIGII
                     RGYSGLLEKQFMGMQSHSVSKIISQGGTILKSSRCPEFKNRSVREKAIENLQEAGIDA
                     LVVIGGDGSFRGANLLCKEFNYPVIGIPGTIDNDIYGTDFTIGFDTAINTAVSAVDKL
                     RDTADSHNMIFFVEVMGRDAGFIALNTAIASGAEATLLPEVHTDIDKLCSFMERDRRK
                     NKTSGIIIVAEGEEEGGAMEVASKVRQKLPNYEIRVTTLGHIQRGGSPTCNDRVLASL
                     LGYEAVNALLKGRTNVMVGQMQNQVVYTPFEEACTRHNEINKTWYEISRILSL"
     gene            6204..7166
                     /gene="dusB"
                     /locus_tag="P5293_02625"
     CDS             6204..7166
                     /gene="dusB"
                     /locus_tag="P5293_02625"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRR48831.1"
                     /translation="MKIAELDLDDKPLFLAPMEGVTDTSFRYICKKFGVDMMYTEFVS
                     SDGLIRDAKSSVAKLEIFEYERPIGIQIYGHIPEAMVEAARLAEEATPSLIDINFGCP
                     VNKIANRGAGSGMMRFPDKMVEITRQIVDAVKIPVTVKTRLGWDENSKIIVQLAERLQ
                     DVGIAALTIHGRTRTQLYKGEADWRLIGEVKNNPRMFIPVIGNGDISSPEDAKEAFDK
                     FGVDGVMIGRATFGRPWIFKEIKYYLSTGEKMEELSLEEKKELAINHLKKSIEVKGER
                     KGVLEMRRHLAAYFKTIPHFRETRRRLVTEDNPDELIKIITNIE"
     gene            7163..8176
                     /gene="lpxK"
                     /locus_tag="P5293_02630"
     CDS             7163..8176
                     /gene="lpxK"
                     /locus_tag="P5293_02630"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRR48832.1"
                     /translation="MKRFLINYVLLFPYFIVLKIRHYLYDSGFLKSKKFDLPVISIGN
                     IEIGGTGKTPHTEYLIKKLMPTERVAVLSRGYGRKSKGFHFVRIDSTVKEVGDEPLQM
                     KRKFVDVIVAVCEKRVKGVEIMLSLPPESRPTVIILDDAFQHRKISPSTNILLVDYNH
                     MLEEQILFPLGRLRDLPEQKKRADIVIVTKCPAEISPEERFVREQHLKLSNNQKLAFS
                     TINYGEPKALFEGADRRYIYSKYAILITAVANPKPLQYQLVSDYKLVERLNFRDHHNF
                     TKRDVEKINKKAAKWPKAVLFTTEKDAQRMMENNNFSALVRERIFYIPIEVEFVSENL
                     NVV"
     gene            8457..11573
                     /locus_tag="P5293_02635"
     CDS             8457..11573
                     /locus_tag="P5293_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR48833.1"
                     /translation="MHVSKKENLSVRRVLLVFALLLLAGPIWAQNIKVTGIVTDIKGE
                     PIPGATVLIQGTKIGEAADLDGKFTITAPSNGILEVSAIGYEKVLIPIKGRASIKVEL
                     RDNAVLLEEAVVTAEFGVKRAARAVGAAVQSVRGEEIAASGRENFVNALQGRIAGVSV
                     TSSSGTPGSSSSVIIRSATSISGNNQPLYVIDGIPMNNSTFNPVGSFAKGSSGSETVS
                     DRQLDFANRGSDLNPEEIESMTVLKGAAAAALYGSDASNGAIIITTKKGRSGKGKVTY
                     NNNFTFSQAYRYPEQQTKYDNGMYGVTNYYTQRRYGAEYPAGTQLYNNLKNFFQTGVS
                     QKHNISFDAGNESMTVRVSAGTLSQTGIVPTSKYTKNNISLAGTAKLANWVNVESSLQ
                     FMETTNTKVSKGTGGPLQRAITWPLTDDMRVYLNDAGSIRYPNRYVDVDILNPYFDLY
                     KNKNYDEGSRFIANMAVNITPIKDLLLKAQVGYDVANTTYEVVRHPEWSSTATGTGSY
                     DQSRVYQSNPTVNLIASYKKDFGKLTVTGTFIYSQLEQFTNTLSVHGEKFLVKDFQSI
                     NNCDKTTLVNLTSRQKRRLQGLSGSFEFGWNNMAFLTVRGRNDWSSTLPQNDNSYFYP
                     AVDLSFIVSELPFMKAMSETFSYVKLRGSVTRVGKDAPPLSIYPALEASTASFGGYRY
                     GFTGPNLKLKPEMNTAWEVGLEARLFRNRMDVDFAYYNTYSADQIITGFRMSYATGFV
                     LNNMNVGTFRTSGVELRVAGDVIKNRDWIVNLGLNMSKNWSNVIKLPENVSEYYNAYT
                     WNSGNLRNGIKVGYPVTSITGLDFQRNKDGKVLIDPSTGLPLVSSTWSVLGDREPDLM
                     FGLTLSVRYKDFSLSALLDGKLGTQVVNGTKRWMMQQGFSKESVKMREAGPVVFNGVL
                     KDGLENSDNPTVNSIGVKLGDLQYGYAGNDYDWIEKGINYLRVQELRLNYNVNRKWLE
                     SVTKGIVSAASIYVMGTDLFVFTNYSGIDVVSNSNSASLGGTGGVGFDMMAIAAPRGL
                     SFGINLTF"
     gene            11586..13376
                     /locus_tag="P5293_02640"
     CDS             11586..13376
                     /locus_tag="P5293_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRR48834.1"
                     /translation="MRKILLSFTVLLAISFTSSCSDWLDVNKNIDSPDWVEPVLRLAP
                     ILEAYEGIPYDLRALAPIVGYMGGSSTYASTFGGSHSYLIASDGGGEAWRMVYWLQGM
                     NLENLIDDAKKLNQSTIAGIGYAVKAFSWHILASLHGDIPVDDAFVPGLLSHDYDNQE
                     YAYQKVREWARLAIAELEKTDQTVYPTLSKYDVVYGGDAIKWKKFAYAVLARNYITLS
                     MKGANYLDSAITCANNSFTSAADDPKIKLEATGVSSTSNFFGVLRGNLANVLVQSDYI
                     VQLLTGTIPNYNAAGSIEGLSPYQNITDTLTLDPRAILLLGTKDTMPADQNSIKLGTY
                     KFIGTRPGYGSNCNFWGLTVSPTAANSGKGRWLYRDDAPWPLTSYAEICFIKAEAQFR
                     KGLKAEAFETFKAGVAAHMDFVKSYIVAGTPVKNTLNKQTSVIGDKITTARFTTLANE
                     YLESNFVNGMSIDNFTLSHIMMQKYVALFPWSYEIWNDIRRYHYDLLLGSSGIPESGI
                     SWTPTTVYHKQDSDASRLFKGFFLPPADVQNRRSKFATANLGSPCYRLRPRYNSEYMW
                     NLPSLKRITPIPGDADNYHTSMVWFCIPNN"
     gene            13400..14227
                     /locus_tag="P5293_02645"
     CDS             13400..14227
                     /locus_tag="P5293_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48835.1"
                     /translation="MKKIFTLISILMVIGLVSCETNEIKYETTPIDQSRFAQVRLIYD
                     LPLVTSSSLNVAWLIYNGDTVSRVSTPLGSIFPNSAAKYHVLPIGTNSVKLLQVNTRA
                     VTYEGTFDLEAGRWSAFVYNTSEPPLMVQEPEYYQTGDPWADTVCYIRFVNLFHKADG
                     TPMGKLYLKGKRTINNVVTYIDIASANYGEASDYVPYKLYRQGIKVWSGTESALVFTV
                     FDENGAQLQYYKTATTKGAYEATGLSMTKGVNYILHFNGKDGTYTTQAYRLSQFTVN"
     gene            14236..14742
                     /locus_tag="P5293_02650"
     CDS             14236..14742
                     /locus_tag="P5293_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HRR48836.1"
                     /translation="MKRFLLIAIMSFVAFSVGYSQNKAEKEAAAQVLFESAKAAIESK
                     DFVIVPDSYENEDGSMETNTDNSHFLAYEGGQYLFLQGSIVCNNNYTNKTTVNSFEQK
                     VDKKGNLSVLMQISGSQINARVEIMVRKGTNYAEVIITPSSGKTKKFSGEVVPRSKAK
                     YYKKSNII"
     gene            14960..>17043
                     /locus_tag="P5293_02655"
     CDS             14960..>17043
                     /locus_tag="P5293_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR48837.1"
                     /translation="MYVSKKEKLTVRRILLVFALLLLAGPIWAQNIKVTGIVTDIKGI
                     PQIGVAVTVSGTTTGTYTDDKGAYSISVPPNGVLQFSAIGMKTQQIQVNNRSVINVVI
                     EEESLMLGELVVVGFGTQKKENLTGSITTVDVSKALQGKPIVDVAKGLQGVVPGLTIT
                     YSNGGINNSPIINVRGMGSINATNKNGGSPLIVVDNVVVDNINLVNPEDIESISVLKD
                     AASTSIYGARAAFGVMLIKTKSGKKNDKFTIFYSSNYAFSTPTLLPEFAKDPVAEIRA
                     TDAAMSRFGGTGMEIFGMTASTLIPGIQKWLSTYKNNRKGDQMIKGEDFDIVNGRVNF
                     FRIWDPVDVMFQSWMPQINQNLQVSGGSDKLSFYVSGAYNYQEGVLKINTDTRNKYNM
                     TVGVNASVNKWLDLEVKISSRQYTYKEPYIGYMDPYYTMWRWGAYMPYGRYTDENGVT
                     GYFRGAHGFLATARKNSEKETYNNSGVNATIKLAKTLNLRTEFAYSTTNRLNYVHGGT
                     VQLWDYWGANPPLVMNTLGTGSTDRVEYSSRRYTQITSNTYATYSESFGDHSVKVIAG
                     VNLENGDYIYHNSQRRTLMDPNTGEIPLATGDQYVDGDHTDWAVAGIFGRINYDYKGK
                     YLLELNGRYDGSSNFPSGSRWAFFPSFSVGYRISEEPWWQNIKDIVSSAKIRGSYGSI
                     GNQDVGADRFIAT"
BASE COUNT         5020 a         3202 c         3434 g         5387 t
ORIGIN      
        1 cgtcattttt ccaattccaa tgtccaacag aaagattacc ttcgaaagag agccatctgt
       61 atggggcaaa tgtgacagtt agttctgttc catagtgcaa tgcatcaagt ccctgaatca
      121 tgaaggagac gtcattatta tcctgttgag tatatggatt tgatacaatt gtcttatttt
      181 tccaatatgc ccaatatccg gtcagttcca gagagcctct ctgaaatata tacctgtatc
      241 ctgcttcgga aagaaagttt ttttcatttt tcacaccgga cgttatttca ttagtacctt
      301 gggcaaagaa aacactggag taaggtattc tgctgtaggc agcagcattg aggtaaatgg
      361 aacttttctt cgtcagttta taatttgctc cgccctttgc gctgaatcct gatactgtta
      421 caagatcact cttgcttcct gttgcaaggc tggagggatc atcaacgtaa ttgtatgtat
      481 cccatcttct ataactgttc cacatataag atagtcctcc tcttatattc catttctcgc
      541 tattatatgc ttctgaaatg tagattgttg cgttattggt aattttaccg tttcttgttc
      601 tgatataatc acctacatgt ttgatcggat tgcgtcctgc tacaccggcc agtgaattgg
      661 tagcatagtc ttcataccag tattctcctc ccagaagatc aattatcttc tccttttccc
      721 aggtagaata gtaaagataa tggacgccac cttcaatctt aagcttatcg ttgatagagt
      781 aggaagtatt gtttctaatg cctgcatgag tgtgtcctgc cagataatta ctgagaatat
      841 ttatagcgga gccagatgct tctgttcctg ttacagcctt gttgtcttct atcacagaat
      901 cccagtctat atgtccatct ttctgatagg aaattattct tcggccttta ctctcagacc
      961 atcttcctcc accatctcca aatgtaaagt ataatgtatt agtcatctct atttttgaat
     1021 ctggtgaata gaaatggttt agagttatat agggtttatg atagaaattt ttgctcaggt
     1081 tttttttctc accattgaga tatccccaat ttttgctgta acggacgcca tatttgtcaa
     1141 cttcatcttt gcttaaacgt acagaccttt gttcatgtct gtcaggagag cccaaaacgg
     1201 tgagcaaaat tttattctca ctgtttattt ttttgctaac attaaatata tatgcccatg
     1261 agtcaacatc tgttgcttca acatatccac ttccacctgt ataggacaac atggctgaga
     1321 ctccccatcc ttttggcaat aatcctgaat tataactaac aaatcctttt gcctgtccac
     1381 ccttgtctgc accgaaagga gagaagaaaa ggcctcctga aactttctgc tcttctgaag
     1441 gagttttcgt aatgatattt atactgccgc caacggagtt gtcagacagc attgaagccc
     1501 caattccctt ttgaacttgg atagaaaatg ttgcatcggc aagcccgagc caattgttcc
     1561 aatacatatt gccactgacc agtcctgaaa ttggaattcc gttcaataag acagaaatat
     1621 tctcctgctt gaatcctctt atgttgattt ttgcatcacc atagcttcct gtttcgcttg
     1681 tggcataaat accgggaatt ccttttagaa gttcgggata tgttcttcca aaagctttca
     1741 tgcttatttc atgctcatca acacttttaa tacgcattgg agatgttgtc tccttgagat
     1801 aggatgcaga cattatgatt ttgtccagga caattgtctt tccaatactg ttttttaaag
     1861 agtcgtaaat ttcgtaatca aggctgtctg gtacctcgct tactgtagag atgcaggtaa
     1921 aatttcctga tgatgtgaag ccgtctgtcc ggcttacaag caaggctaag ataaccatcg
     1981 aaaccttgcg tgaaaggttt ataatatttg tttccataaa ttcttcttcc ttccagactt
     2041 taactgtcgg taccgtattt aaacggttct gcctctaatg gctcgcggac tttaccgccg
     2101 atcgggaatt tcaccctgcc ctgaagattt aggtcgcaaa tgtaataaat attttttaaa
     2161 agcgtcaaaa aaaggcatta cgcttgttga tagtaagaaa ataaagcata ttaaaaaata
     2221 tggctgccaa ttaaattggg cagcctctat tagtatgtag acaaataatt gttaacaaat
     2281 acagcaatta tcagtcatta agtgaaaaac gagaaaattc ggtaaccctg acctcgggat
     2341 cgacactttt cagatatgca gcaacagtaa ttttgccgtc tttaacaaat gtctgttcct
     2401 caagtgtgct ttctttgaaa aatttgttga gtttgccttg tgctatttgc tcaaccatat
     2461 tctcaggctt accctcttcg cgaatctgta tacggtatat ttcgagttcc ttgtcaataa
     2521 cctctttagg acaactctct ctgctaactg aaacaggaga catggcagtt atttgcatag
     2581 caatctcttt tccaacttca tggcttacag tttttgagaa tccaacaatt gttgcaactt
     2641 tgccatttgt gtgtatgtag tagtcaacgt atggagcctc tattctactg tagaagagaa
     2701 gagcatgttt ttcaccactc ttgccagtct gttgtgtaat agcctcctca atagtcatat
     2761 tatctatttt tagagatttt aaagcttcca gatctgcagg aaaagattcg agtgctacat
     2821 cagcaatttt atttgcgagt tcttgaaaat ccgcattcct tgacacaaag tcagtttcac
     2881 aaccaagaca tataagaatt gctttattat tcgaagaatt tactcttgca attacagaac
     2941 cttcggagat ctctctatcg gctctttttg ctgcgataag ctttcctttt tctctgataa
     3001 tatcttttgc tttttcataa tcaccgttgg cttcgacaag agcatttttg cagtccatca
     3061 tgccggcccc ggtcatttga cgtaatttag caacgtctgc tgcttttatt tccatatcaa
     3121 ttatttgtta atttttaata ttaaacccgc ttttaattac tatgattcag tagtttcgtc
     3181 aacaggtttc tcttcaatag cagtgggttt ctctccttca ttttcattct ttgcattctt
     3241 ccttgggcgc atcttccttc cactctcctt gacatttacc tcaccactat ccttgccact
     3301 ctcggcactt tctttttcct tttcaaactt tctctcggca agaccctctg atatagcctg
     3361 tgcaagaaca cccattataa gtgagatgga ttttgctgca tcatcgtttg ccggtatcac
     3421 gtaatcaatt tgacttggat cgcaacatgt atcaaccata gcgattacgg gaatgttaag
     3481 acgatttgct tctttgacgg cattcatctc tttttgaata tcaactacaa acagtgcaga
     3541 aggcagacgg tttaaatctg ctattgaacc aaggtttttt tcaagttttg ctctttgacg
     3601 ggtaatttga agtttttctc tcttggcaag agtctcaaaa gtaccgtcag tcatcatttt
     3661 gtcaatggtg ttcatcttct tgactgcctt acggattgta gggaagtttg taagcattcc
     3721 acctggccat ctttcagtta catatggcat attaactgat ttggcatatt cggcaactat
     3781 ctcttttgcc tgtttttttg tggccacaaa cagaattttt cttccggcac gtgccatctg
     3841 tttcattaca tatgcagcct catctatctt gataatggtc ttgtgcaggt caataatgtg
     3901 gatcccgttc ttttccatga agatataggg agccattttg ggattccatt ttctttttag
     3961 gtgtccaaaa tgtacacctg catcaagtag ttcttggaaa gttgttctag gcatttttat
     4021 ttcttttttt gatttttaaa ttctcttttc atcaaccgag agtaacgttt ttgtttttaa
     4081 gttcaaatta gccggtctct caagttttcc gcagttggac aaacaatggt aacacatatc
     4141 aatcaaacag ttatgaggtc caattgtttt caatccaatc tgtacaattg tcagcagtta
     4201 tttgcttgca agttcaagac taacgtttgc tgaattggaa gcgcttacgt gcaccgggtt
     4261 gtcctggttt ctttctctca acctctctcg aatcgcgggt aagaaatccg tttgatttaa
     4321 gtacaggtct gtaagcttct gtgtctatct tacaaagggc acgggcaata gcgagacgaa
     4381 gtgcttctgc ctggcctttt atacctcctc cgcagagatt gactcttata tctaactgag
     4441 acagagtgtt agttaattcg agcggttgcc ttacaatata cttgagcgag tcgtctctga
     4501 aataactgtc aagatctctt ccgttaattg taatgttacc tttcccggag tttacataaa
     4561 tgcgagcaac tgctgttttt cgtcttccaa gggcgttaat tacttccatg ctctgttaaa
     4621 tttcatttaa attaataact ttaggctgct gagcttcatg aggatgctcc ggaccttcat
     4681 atacatacag gtggcggaac atctcggctc cgagcctgtt tttaggcagc atgcctttga
     4741 ttgcatgctc aactacggcg agaggctttc gagccagtaa ctccttagga gtagaaaatc
     4801 tttgcccgcc gggataaccg gtgtgacgaa cgtacacctt gtcggtcaac ttttttcctg
     4861 tcaggcgaac tttggaggcg ttaataatta ttacattatc tccacagtcc acatgggggg
     4921 taaagttggg cttgtgttta ccgcgcagga tgagggcaac tctcgatgca agtctgccaa
     4981 gaatagctcc attggcatct ataaccatcc agtccttgtt aactgttgcc ttatttgccg
     5041 acacggtttt gtaactcaat gtgtccactt tgttgatttt tagattaata catttatttt
     5101 gttgcgattg ccccataaaa tagggggctg caaagataaa actatttttt aaagtgacaa
     5161 aatgcgactt atctcatacc atgttttatt aatctcatta tgtcttgtgc atgcttcttc
     5221 aaaaggagta taaacgactt gattttgcat ttggccgacc attacatttg ttctgccttt
     5281 taggagtgca ttgacagctt cataacccag aagactggca agtactctat cgttgcatgt
     5341 gggagagcct cctctctgga tatgcccgag agttgttaca cggatttcat agtttggaag
     5401 tttttgtcta accttcgatg caacttccat agcgccacct tcttcttctc cttcggcgac
     5461 aataattatc ccggatgtct tatttttgcg tctgtctctc tccatgaagc tgcaaagctt
     5521 atcgatatca gtgtgaactt cagggagcag ggtggcttca gcaccacttg caatagcagt
     5581 attgagtgca ataaatccgg catctcttcc cattacctct acaaaaaaaa tcatattgtg
     5641 agagtcagct gtatctctga gtttgtcaac ggcagaaacg gccgtattga ttgcagtgtc
     5701 gaaaccaatt gtaaaatcgg taccataaat atcattatca attgtccctg ggatgccaat
     5761 taccggatag ttaaattctt tgcagagaag attggctcct ctgaatgaac cgtctccgcc
     5821 aattacaacc aatgcatcaa ttccggcttc ctgcaagttt tctattgctt tttcccggac
     5881 gcttctgttt ttaaattcag gacaacgaga tgattttaag atagtgcctc cttgagatat
     5941 aattttcgaa actgaatggg actgcatacc cataaattgt ttttcgagca gtcctgaata
     6001 gcctctgatg attccaatca cttccagttg attgtagatt gcagttctga cgacagccct
     6061 aatggctgca ttcatccctg gagcatcacc tcccgaagtg agtactccaa ttctgtttat
     6121 tttccttttt gagttcatga tattgagcaa atcgtggcaa aattactttt ttttattgtt
     6181 attctaattt ctacttttgc aacatgaaaa tagctgaact ggatcttgat gataagccct
     6241 tgtttcttgc acctatggag ggtgtcactg atacgtcatt tagatatatt tgtaagaagt
     6301 tcggtgtcga catgatgtac actgaatttg tttcttccga tggcctgata agagatgcaa
     6361 aatcgtctgt tgccaaactc gaaatatttg aatacgaaag gccaataggt attcaaattt
     6421 acggacacat acctgaagcg atggttgaag cagccaggct tgcagaggaa gctacccctt
     6481 ctctgattga catcaacttt ggttgtccgg ttaacaaaat agcaaacaga ggtgccggtt
     6541 ctggaatgat gagatttcct gacaaaatgg tggagattac aaggcaaata gttgatgcag
     6601 tgaagattcc cgtaacagtt aaaacaagac taggatggga cgaaaattcc aagattattg
     6661 ttcaattggc cgagaggctt caagatgtgg gtattgcagc attaacaatt cacgggagga
     6721 cccggacaca actctataaa ggcgaggcag actggaggct tatcggagaa gttaaaaaca
     6781 atccgagaat gttcatacct gtaataggta atggagacat atcttctccc gaggatgcaa
     6841 aagaggcatt tgataaattt ggtgttgatg gagtaatgat aggcagggcg acttttggaa
     6901 ggccctggat attcaaagag ataaaatatt atttatctac tggagaaaaa atggaagagc
     6961 tttcacttga agaaaaaaag gaattggcaa ttaatcatct caaaaaatca atagaggtaa
     7021 aaggtgaaag aaaaggagta ttggagatgc gcaggcatct tgctgcttat ttcaagacca
     7081 ttccgcactt tagagagaca agaaggcgac tggtgaccga agataacccg gatgaactta
     7141 taaagattat aacaaatata gaatgaaaag atttctcatt aattacgttc ttcttttccc
     7201 atattttata gtgcttaaaa tcagacatta tttatatgat tccggttttc ttaaatctaa
     7261 aaaatttgat ttgcctgtta tatcaatagg caacatagaa attggaggaa cgggtaaaac
     7321 tcctcataca gagtacttga taaagaaatt aatgccgaca gaaagagttg ctgtcttgtc
     7381 tagaggatat gggagaaaaa gtaaaggatt tcattttgtc cggattgaca gtacagtcaa
     7441 ggaagttggt gatgaacctt tgcagatgaa gaggaaattt gtagatgtaa ttgtggcagt
     7501 gtgtgagaag agggttaaag gtgttgaaat tatgctttct cttccaccag agtcgagacc
     7561 tactgttatt atacttgatg atgcttttca gcacaggaaa atatcacctt ctacaaatat
     7621 acttctggta gattacaatc acatgctcga ggaacaaata ctgtttccat tagggcgatt
     7681 aagggattta ccggagcaga aaaaaagagc agatatcgta attgtaacta aatgtcccgc
     7741 tgagatttct cccgaagaga gatttgttag agaacaacat ttgaaattgt caaataatca
     7801 aaaattggca ttctcgacta ttaattatgg agaaccaaaa gctctttttg aaggtgcaga
     7861 cagaagatat atctattcta aatatgcgat actaattact gctgtagcaa atccaaaacc
     7921 tttgcagtac caattagtat cggattataa gttagttgag aggctgaatt ttagagatca
     7981 ccataatttt actaaaagag atgttgaaaa aataaataaa aaagccgcaa aatggccaaa
     8041 agcagtgctg tttacaactg aaaaagatgc tcagagaatg atggaaaaca acaatttttc
     8101 tgctttagtt agagaaagga ttttctatat acctatagag gtcgaatttg taagtgaaaa
     8161 tttaaatgtt gtttagattt gtaacttatc taattttata tgttttaaac tgtaagctaa
     8221 attcaaattt tggagataaa ttatttagtt gccaggttgt taaatttttg ttatttttct
     8281 atgaatcaag gtattatctg ccctttattt aatgatacaa taattattgc attttaacat
     8341 aatttctttg aataattagt agtaaatgtt atataaattt ttaaatttgt gcatgttgtt
     8401 aataaagaaa attaacgact aataattact aaattactaa cctaaattat taaactatgc
     8461 atgtttcaaa gaaagaaaac ctatcagtac gaagagtact gttggtcttt gccctcctgc
     8521 ttttagcagg tcctatctgg gcacagaaca tcaaggttac cggtatagtt actgacatta
     8581 agggagagcc tattccaggt gcaactgtct tgattcaggg gacaaaaatc ggtgaagctg
     8641 ctgaccttga tggtaaattc acaataactg caccgtcaaa tggaatactc gaagtttctg
     8701 ctatcgggta tgaaaaagtt cttattccca tcaaaggcag agcctctatt aaggttgaac
     8761 tgcgagataa tgccgtttta cttgaggagg cggttgtaac agccgaattt ggagttaaaa
     8821 gagctgcaag agctgtcggc gcagcagtac aaagtgtcag gggagaggaa atagctgcat
     8881 ctgggcgtga aaatttcgta aatgctctcc agggtcgtat tgcaggtgtt tcagttacca
     8941 gttcaagtgg gacgcctggc tcttctagta gtgttattat tcgaagtgca acttccattt
     9001 caggtaataa ccagcctcta tatgttattg atggtatccc gatgaacaac tctaccttta
     9061 atcctgtagg ttcttttgca aaaggctcaa gcggctcgga aacagtaagt gacagacagc
     9121 ttgactttgc taaccgtgga tcagacctaa atcctgaaga aatagagagt atgacagttt
     9181 tgaagggagc cgctgccgca gctctttatg gcagtgatgc ctctaatggt gctataatta
     9241 ttaccacaaa aaaggggaga agtggtaagg gtaaagttac ttacaacaac aattttacat
     9301 tttctcaggc atacagatat cctgaacagc agaccaagta tgacaacggt atgtatgggg
     9361 taacaaacta ttacacacaa agaagatatg gagcagaata tcctgctggt acacagctct
     9421 ataacaatct caagaacttt ttccagacag gtgtaagcca aaaacacaat atctcttttg
     9481 atgcaggaaa tgaatcaatg actgtacgag tttctgcagg aacactttca cagacaggta
     9541 ttgttcctac atctaaatac accaaaaaca atatctctct tgccggtaca gccaaacttg
     9601 ctaattgggt taacgtggag tctagtttgc agtttatgga aacaaccaat accaaggttt
     9661 ctaaaggtac cggtggccct cttcaaagag caattacctg gcctcttaca gatgacatga
     9721 gagtatacct caatgatgca ggcagtatca gatatccaaa cagatatgtt gacgtcgata
     9781 ttcttaaccc gtacttcgac ctttacaaaa ataaaaacta tgatgaggga tcccgtttta
     9841 ttgccaatat ggcggtaaat atcacaccta ttaaagacct tcttctaaaa gctcaggttg
     9901 gctatgatgt ggctaataca acatatgaag tagttcgcca tccagaatgg tcttcaacag
     9961 ccacaggcac tggttcttat gaccagtcga gagtttacca gtcaaatcct acagtcaatc
    10021 ttatagcttc ctataaaaag gattttggaa aattaactgt aacaggtaca tttatttatt
    10081 cgcaactcga acagtttaca aacaccctct ctgttcatgg tgaaaaattt cttgtcaaag
    10141 attttcaatc aattaacaac tgtgataaaa ccacacttgt aaatttaaca tcaagacaaa
    10201 agagaagact tcaaggtttg tccggttcat ttgaatttgg ctggaacaat atggcctttt
    10261 taactgtccg tgggcgtaac gactggtctt ctacactccc tcaaaacgac aacagctact
    10321 tttatccggc tgttgacctt agctttatcg tttctgaact cccttttatg aaagcgatgt
    10381 cagagacttt cagctatgtt aagttgagag gttctgttac cagagtcggt aaggatgctc
    10441 ctccactttc catatatcct gcacttgaag catctactgc atcatttggg ggatatagat
    10501 atggttttac cgggccaaat ttgaaactta aacccgagat gaatactgca tgggaagtag
    10561 gtttggaagc tcgtctattc agaaacagga tggatgtgga ttttgcttat tacaatacat
    10621 attctgcaga tcagattatt accggtttca gaatgagtta tgctactgga tttgtgctta
    10681 acaacatgaa cgttggtaca ttcagaacca gtggagttga gttgagagtt gcaggtgatg
    10741 ttataaaaaa ccgtgactgg atagtgaatc ttggacttaa tatgtccaag aactggagta
    10801 atgtcattaa acttccagaa aacgtatctg agtactataa tgcatatacc tggaattcag
    10861 gaaacttgag aaacggaatt aaagtcggct atcctgttac ttcaattaca ggtttggact
    10921 ttcagcgcaa taaagatgga aaagtactga tagatccttc taccggtctt cctcttgtat
    10981 caagcacttg gtcggtgctt ggcgaccgcg agcctgacct catgtttggt cttacactct
    11041 ctgtgaggta taaggatttt tctctatcag ctctgcttga tggaaaatta ggtacacagg
    11101 tggttaatgg cactaaacgc tggatgatgc aacaaggatt cagtaaagaa tctgttaaga
    11161 tgagagaagc agggccagta gtgtttaacg gagtgttgaa agatggttta gagaactctg
    11221 ataaccctac agtcaacagc atcggagtta aattgggaga tcttcagtat gggtatgcag
    11281 gaaatgatta tgactggatt gaaaaaggta ttaactattt gagagttcag gaacttcgtt
    11341 taaattacaa tgttaacagg aaatggcttg agagtgtaac aaaagggatt gtttctgctg
    11401 cttcaatata tgttatgggc actgaccttt ttgtattcac aaattattca ggaattgacg
    11461 tagtaagtaa ctctaactct gcatcactcg gcggtaccgg tggagtaggg tttgacatga
    11521 tggcaatagc tgctccgaga ggcttgtcat ttggtatcaa tctaacattt taacggatta
    11581 aagctatgag aaaaatatta ttatctttta ctgtacttct ggcaattagt tttacctctt
    11641 catgctctga ttggctggat gtaaataaaa atattgactc cccggattgg gttgagcctg
    11701 ttttaaggct tgcgcctatt cttgaagctt atgaggggat tccttatgat ttaagagctt
    11761 tggctcctat agttggttat atgggaggaa gctcaacata tgcaagcaca tttggtggaa
    11821 gtcactctta ccttatagca agcgatggtg gtggagaagc ttggagaatg gtatattggc
    11881 ttcagggtat gaaccttgaa aatttgattg atgatgccaa aaagttgaac cagtcaacga
    11941 ttgcaggtat tggctatgct gtcaaagctt tttcctggca tattcttgct tctctgcacg
    12001 gagatatccc tgtagatgat gcatttgttc ccggacttct ttcacatgac tacgataatc
    12061 aggaatatgc ctatcaaaaa gtaagagaat gggcaagatt ggctatcgcc gaacttgaaa
    12121 aaacagacca aactgtctat ccaacattat ccaaatatga tgttgtctat ggaggagatg
    12181 ccataaaatg gaaaaagttt gcctatgcgg tgcttgcaag gaattatatt actctgtcta
    12241 tgaaaggtgc aaattatctc gattctgcaa ttacttgtgc aaataattca tttacaagtg
    12301 ctgcagatga ccctaaaatt aaacttgaag caaccggagt atcttccaca tccaatttct
    12361 ttggagtttt aagaggcaat cttgccaatg tcttggtaca gtctgactat atagtacagc
    12421 ttcttacggg aactattcca aactacaacg ctgccggctc tattgaaggt cttagtcctt
    12481 accagaatat aactgataca ttgacactcg atccgcgtgc cattcttttg cttggtacta
    12541 aggataccat gcctgctgat caaaacagca taaaactggg tacatataaa tttatcggga
    12601 ctagacctgg ctatgggtca aattgtaact tctgggggtt aactgtatca ccaactgcag
    12661 caaattcagg taaaggaaga tggttatata gggatgatgc cccatggccg ttaacctcat
    12721 atgctgaaat atgctttatc aaggcagagg ctcaatttcg caaaggcctc aaggctgagg
    12781 cttttgaaac tttcaaggcc ggtgtggcag ctcacatgga ttttgtaaag agttatattg
    12841 ttgcaggcac acctgttaaa aatactttaa acaagcaaac ttcagtcata ggagataaaa
    12901 ttacgacagc tagatttact actcttgcca atgagtattt ggaaagcaat tttgtaaatg
    12961 gcatgtctat cgacaatttt acactctctc atataatgat gcaaaagtat gttgcattat
    13021 tcccttggag ttatgagatt tggaatgata tcagacgtta tcattatgac ctgttgttgg
    13081 gatcaagcgg tataccggaa tcgggtattt catggactcc tacaacagtt tatcacaaac
    13141 aggacagtga cgcatcaaga ctttttaaag gctttttcct tccccctgca gatgttcaaa
    13201 acagaagatc aaagtttgcg acagcaaact taggatcacc atgttacaga cttcgtcctc
    13261 gatataattc agagtatatg tggaatcttc cttcactgaa aaggattact cctattccgg
    13321 gtgatgcaga caattatcat acctcaatgg tgtggttctg tattccaaat aattaagaat
    13381 gactaaaaaa ggaatgataa tgaaaaaaat atttacatta atctcaattt taatggttat
    13441 agggctcgtc tcatgtgaaa ccaacgaaat caaatatgag acaacaccta tagatcagtc
    13501 cagatttgca caggtaagac tgatctatga tttaccattg gtcacatcat cttctttgaa
    13561 tgtggcatgg cttatttaca atggagatac agtttccagg gtttcaactc cgttaggcag
    13621 catttttcct aacagcgctg ccaagtatca tgtattgcct attggcacca actctgttaa
    13681 actgcttcag gtcaatacac gtgcggtgac ttatgaaggt acttttgatc ttgaagcggg
    13741 tagatggagt gcatttgttt acaacacatc tgaacctcct ttaatggttc aggagccgga
    13801 gtattaccaa acaggggatc cgtgggctga tacagtttgt tatatcagat ttgtcaattt
    13861 atttcacaag gcagacggta ctcctatggg caaactttat ttaaaaggaa agagaactat
    13921 taacaacgtt gtaacttata ttgatatagc aagtgccaac tatggagagg cttctgacta
    13981 tgtgccttat aaactttatc gtcaagggat aaaagtttgg agtggtactg aatcagctct
    14041 tgtgtttaca gtgtttgatg aaaacggtgc acagttgcaa tattacaaaa cggctactac
    14101 aaagggtgct tatgaagcaa caggtttgag catgacaaaa ggggtgaatt atatattgca
    14161 ctttaacggc aaggacggaa catacacgac acaggcctat agactgagtc aatttacagt
    14221 aaattaattt aaaatatgaa aagatttttg ttgatagcga ttatgtcttt tgttgcattt
    14281 tctgttggct actctcaaaa caaagcagaa aaagaggctg cagcacaggt gctttttgag
    14341 agtgcaaaag ctgccataga atctaaagat tttgttattg tacctgacag ttacgagaat
    14401 gaggatgggt caatggagac caatactgat aattctcatt ttcttgccta tgaaggaggt
    14461 caatatcttt ttcttcaagg gagtatcgtg tgcaataaca attacacaaa caagacaaca
    14521 gtcaatagtt ttgagcagaa ggtcgacaaa aagggcaatc tttccgtact catgcaaata
    14581 tccggttccc agatcaatgc cagagttgaa attatggtga gaaagggaac aaactatgca
    14641 gaggtaataa tcactccttc cagtggcaaa acaaagaagt tctctggaga agtagtccca
    14701 aggagtaagg caaagtatta caaaaaatca aacataattt agatttaata ttatcctatc
    14761 atgttataat taagagtgtc atattatttt gacactcttt ttattttatt cattttttct
    14821 tatatttaca taaatttggt taaaatctaa aattatccgc tctgcatttc tatacgtaag
    14881 atttgttttc tttataagtg tatgacactg ttctatttta ctaacattgt tcaacttcat
    14941 taacctaatt ttttaaacta tgtatgtttc aaagaaagag aaactgacag tacgaagaat
    15001 actgttggtt tttgccctcc tgcttttagc aggtcctatc tgggcacaga acatcaaggt
    15061 taccggtata gttactgaca tcaagggaat tcctcaaatt ggtgttgctg taactgttag
    15121 tggcactaca accgggacct atactgatga taaaggcgcc tattcaattt ctgttcctcc
    15181 caatggagtt ctccagtttt ctgcaattgg gatgaaaacc cagcagatac aggtaaacaa
    15241 ccgttctgta atcaatgttg tgattgaaga agaatccttg atgttgggag agttggttgt
    15301 tgtaggtttt ggtactcaga agaaagagaa ccttacaggt tctattacta cagttgatgt
    15361 atcaaaagct cttcagggga agcctattgt tgatgttgcc aaaggtcttc agggagtggt
    15421 tcccggtttg actattactt attctaacgg tggaatcaat aattcaccta ttattaatgt
    15481 ccgcggcatg ggatcaatta acgcaacaaa caaaaatgga ggttctcctc ttattgttgt
    15541 ggacaatgtt gttgtggata atatcaatct tgtcaatccg gaggatatcg agtccatatc
    15601 agtattgaag gatgcagctt ctacatctat ttatggagca agagctgctt ttggagtgat
    15661 gctgataaag acaaaatccg gaaagaaaaa cgacaaattt actatctttt attcaagtaa
    15721 ctatgctttt agtacgccta ctttgcttcc tgaatttgcc aaagatcctg tagctgagat
    15781 acgagctact gatgctgcaa tgtcgagatt tgggggtaca ggtatggaaa tattcggtat
    15841 gactgcatct acccttattc ctggcattca aaagtggttg agcacgtaca agaataacag
    15901 aaaaggtgat caaatgatta agggagaaga ttttgacatc gtaaacggca gggtcaattt
    15961 ctttagaatt tgggatcctg tagatgtaat gtttcaatcc tggatgcctc agatcaatca
    16021 gaacttacaa gtttcaggcg gctctgacaa gctgagcttt tatgtttcag gagcatataa
    16081 ttatcaggaa ggagttttga aaatcaatac tgatacacgt aacaagtaca atatgacagt
    16141 tggtgtaaat gccagtgtca ataaatggct tgatttggaa gttaagatta gtagtcgtca
    16201 gtatacctac aaggaacctt atatcggata tatggaccca tattatacaa tgtggcgttg
    16261 gggtgcttac atgccttacg gaaggtatac tgacgagaat ggcgtaactg ggtatttcag
    16321 aggggcacat ggcttccttg ctacagcaag aaaaaattcc gaaaaagaga catataacaa
    16381 ctctggagtt aacgcaacca ttaagcttgc caaaacgtta aatttaagga cagaatttgc
    16441 ctacagtact actaaccgtc tgaattatgt tcacggaggt actgtacaac tctgggatta
    16501 ctggggtgct aatcctcctc tggttatgaa tacacttggt actggttcta cagacagagt
    16561 tgagtattct tcaagaaggt acacacaaat aacatccaat acctatgcta cttactctga
    16621 gtcttttgga gaccactctg ttaaggttat tgcgggtgtg aacttagaaa atggggatta
    16681 tatttatcat aacagtcaga ggaggacatt aatggatccg aatactggag aaattcctct
    16741 tgcaactgga gatcagtatg ttgatggaga ccacaccgat tgggctgttg caggtatttt
    16801 tggaagaata aattatgatt ataaaggtaa atatctgctt gaacttaatg gcagatacga
    16861 tggttcgtca aatttccctt caggttcaag atgggcattc tttccttcat tctctgtagg
    16921 atatcgtatt tctgaagaac cttggtggca gaacataaaa gatattgtta gcagtgcaaa
    16981 aatcagaggt tcttatggca gtattggcaa ccaagacgtt ggtgctgaca gatttattgc
    17041 aac
//
LOCUS       DAOTQL010000023        16461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3393_length_16461_cov_4.901378, whole genome shotgun sequence.
ACCESSION   DAOTQL010000023 DAOTQL010000000
VERSION     DAOTQL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3393_length_16461_cov_4.901378"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..908
                     /locus_tag="P5293_02660"
     CDS             78..908
                     /locus_tag="P5293_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRR48838.1"
                     /translation="MNIPKYINEKHNIVKLVSLTALFALIFINIYKPFSSESWYEVSD
                     FMFFVYSSLIILTGVLVVIISRIIMYFYNRKHEISYSLYVFWVILEIIFMSLFYTIYT
                     LSVKDGADVMETFKNSIINTSLVLLLPYATLWIYMGWKEAQKKLQKIEMDEEDINPLK
                     TIAFKDEKGQVMLSIPLQDLLYVESSNNYVEIHYTSNGKNKKYLLRNTLKNIENLLEN
                     TSVGRCHRSYLVNFGRVKVMRREKGGLFLELDAEGVMDIPVSKSFQDKVGEKFVSNSF
                     "
     gene            975..3233
                     /locus_tag="P5293_02665"
     CDS             975..3233
                     /locus_tag="P5293_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR48839.1"
                     /translation="MNIVSGKLRIIFVCNLIFGMTALPAFSQIKIDSVIVTGYLKGGI
                     LEEQLVPISKINLQKLENENVTALKGLSGSAPNFYIPDYGSRMTSSVYLRGIGARIDN
                     PVIGLYVDGIPYLNKNNFDFDFFDIRSVDILRGPQSSLFGRNTIGGVVNITTLSPFAY
                     QGTKASIGYGNGNSFSIQASQYSKCNDRLGISAGAYYNSSDGFYTNDFSHNDLDWYKG
                     GGVRLRLQWIKSADLSFDNSFTIGYLEQGGFPYGKVDTLTWSASNPNYNDKSGYKRFT
                     VSEGVSFKKNLDKYILTGIISYQYTDDKMNMDQDYTPDSYFSLIQNQKEHTIYQDIVL
                     RSKEDNRWKWLNGASCFFKYLDMDAPVTFKEDGIDRLILANANKGLSLMFPGEEIKIK
                     EENFVINSFFKIPTYGVALYHHSFFRYGKWLFEAGLRLDCEGSHLRYDNGADIHYLFT
                     MTMSEYREIVTKLKGNENLLFIEILPRVAASYNTGNYKIFSSLARGYKAGGYNTQLFS
                     DILQNQMMEDMMNDLGVHLTNSAYSAYNIADVITYKPEYCLDFELGVNYSSNTGFNFN
                     LLFFDIICTNQQLTIFPERNSTGRMMTNAGKTRSYGVEGSISYNWKNLKLYGDYGYTD
                     ARFIKYNNGRNDFSGKYVPYAPQQTVSAGVDYTILAKNNQLLPDKIVIHADVKGFGKI
                     YWNETNTLAQNFYTLLNGSISLQKKPFTLELWGKNLLSSDYSTFYFLSMGNSFLQKGK
                     PLQYGLKFKINI"
     gene            3244..3720
                     /locus_tag="P5293_02670"
     CDS             3244..3720
                     /locus_tag="P5293_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48840.1"
                     /translation="MKTFKTIKFSVIIAFAVVSFFASSCEKDDNEPIVITEDITFSGR
                     MTVDQLDGTNYTVNGVQIKIEPATSGNILTIEMLQVSFSPKMPVKLDMKIQEVMYYSS
                     SKKIIISGNNIIPIAMGGEFPAYTITNLSGEIENGSITLSMMCGNYPLTYSGTLQL"
     gene            complement(3715..4317)
                     /locus_tag="P5293_02675"
     CDS             complement(3715..4317)
                     /locus_tag="P5293_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRR48841.1"
                     /translation="MGTHGNLRLAVIDENIVTCVGLRTILNEVVPFAQLSIYTDINTF
                     IEDRPEEFFHYFVSAGIFIQNQVFFTERNKKTIVLTAKDEDCQIPDTIRIINVTQAEE
                     SLIKSILSIHQNAHSNGYHVQEAPVQNKAPRLLSNREAEVLTLVVKGFMNKEIADRLN
                     ISITTVITHRRNITDKLNIKSVSGLTIYAVTHGYIDIETL"
     gene            4471..5325
                     /locus_tag="P5293_02680"
     CDS             4471..5325
                     /locus_tag="P5293_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR48842.1"
                     /translation="MKKEKIIKAPNGYLMFAVLLVMVAFTIYFLVNNLYLIGIIMLVI
                     DIFMLPGFFVINPNSSKVLLLFGAYRGTIKVNGFYWVIPFYTRFGVSLKARNFESERL
                     KVNDKLGNPIMISSILVWKVDDTYKAMFEVDDYEAFVKVQTDAAVRKLAGSYPYDQFE
                     DEKASVTLTSNFEEVSKSLETEISERLKLAGITVIEARIGYLAYSQEIAQAMLKRQQA
                     TAIIAARSKMVEGAVGMVEGALRLLSSKKVVELDEDKKATMVSNLLVVLCGDKETSPV
                     INTGTLYQ"
     gene            5337..5510
                     /locus_tag="P5293_02685"
     CDS             5337..5510
                     /locus_tag="P5293_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRR48843.1"
                     /translation="MGEKKSFVLRIDPETMKAIEKWAADEFRSINGQLEWIINKSLKE
                     AGRLKEDKKESET"
     gene            5605..6837
                     /locus_tag="P5293_02690"
     CDS             5605..6837
                     /locus_tag="P5293_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRR48844.1"
                     /translation="MWRKWLLIIGILLTSQLSIAGNPEDTIPTSNRYIHQLETEVRQA
                     YIFPSTSFLEGENNKSKALRSAFSAHLRYAFSFPPLSYIDRIYGSPYQGIGVAYYNFG
                     DRHEIGNPIAVYLLQGGRLADFSSRLSLNYEWNFGISAGWHPYDVNSNPNNTVIGSKV
                     NAYLNTSAYLNFTLSPQFDLTSGISLTHFSNGNTDFPNAGLNTIGIKMGLTYNFKKNK
                     AIQLNNTPPVLISDFPRHFSYDLIFFGSWRRKGIVYNDIPLASPYTYEVFGFNFTSFY
                     NIRYRFRAGISLDGVYDSSANIYTEEYNVTADGHDPSFTFYKPPLKKQLSLGLSARAE
                     YIMPYFSINMGLGTNIIHGGGDLRGLYQIVALKIETSRNSFIHIGYNLHDFKEPNYLM
                     IGFGYRFHNKYPFIYHSF"
     gene            complement(6861..7562)
                     /locus_tag="P5293_02695"
     CDS             complement(6861..7562)
                     /locus_tag="P5293_02695"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRR48845.1"
                     /translation="MSIKENIDFQLKALAEEKYRFFNQKIVPTRYELLGVRMPSLKKL
                     AKELSTDNEIEVYLKNALKSEDQSYEHIILYGLILGELKNISIETLFTYLDPLIYKFD
                     NWAHVDSVVSALKIFKKSPDEAFSHFLPLKEDDGEFTKRFFVIFMMDYLMDATHIDAT
                     LKHLSQVRQGQYYVDMGIAWALSNGLVKFYDKTVRYLENRSFSEFVHNKTIQKACDSF
                     RIASVTKGLLKNMKI"
     gene            complement(8017..8089)
                     /locus_tag="P5293_02700"
     tRNA            complement(8017..8089)
                     /locus_tag="P5293_02700"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8054..8056),aa:Phe,seq:gaa)
     gene            complement(8158..8230)
                     /locus_tag="P5293_02705"
     tRNA            complement(8158..8230)
                     /locus_tag="P5293_02705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8195..8197),aa:Pro,seq:cgg)
     gene            8269..8835
                     /gene="efp"
                     /locus_tag="P5293_02710"
     CDS             8269..8835
                     /gene="efp"
                     /locus_tag="P5293_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRR48846.1"
                     /translation="MATTADFKNGLCIDFNGKPCSIIWFQHVKPGKGGAFVKVKMRNL
                     ENGKILENTYTAGERIEILNVERRPYQFLYKDDMGFNFMHTETFEQITLDPEMVENAD
                     LMKEGQYVEMMFLADQERALTCELPPFVELEVTYTEPAVKGDTASTNALKAATLETGA
                     EIMVPLFINQGERIKVDTRDRSYYERCK"
     gene            8846..10294
                     /locus_tag="P5293_02715"
     CDS             8846..10294
                     /locus_tag="P5293_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRR48847.1"
                     /translation="MKHKSIFLPFLFLLIISCSGEKSYQLPAIAHINNEISSGNFTEA
                     SKLIKIRLMSDTLPEIERWNLNFELEKMDRIRYDFRASDSSVISYIKRYYPNVTKSEI
                     EHWEQTNALENMIIDGKKCYFRSAARNLFRIDSIAAKHFVAAEGQAGDSLTRFLAKHI
                     PQLISERQNVNGTYFKPVTMNVKYTLSVKPNEVPDGEIVRVWMPYPRGDVRSQSNIRL
                     ISTSQPDFLISPDKYLHKSIYMEKKAVKDSATIFSYNLEYTCYSQHFNFDPNNIKPYN
                     IEDSIYKKYTSEIPPHIVFSNKIKDTVAKIVGSETNPYKKIKLIYEWIDSNFPWASAR
                     EYSTIENIPEYVLANRHGDCGQVSLLLITMARCAGVPAKWQSGWMMHPGNLNLHDWAE
                     IYYDGIGWVPVDQSFGRVKSALGNDEVYYFYTKGIDAYRMIVNQDISAPLFPAKIYPR
                     SETVDFQRGEVEWKGGNLYFDRWNYDMEVKYH"
     gene            complement(10310..10939)
                     /locus_tag="P5293_02720"
     CDS             complement(10310..10939)
                     /locus_tag="P5293_02720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRR48848.1"
                     /translation="MTKEEKYIFDNSSPLSDALVWLERETNLRTSHARMLTGAVQGRF
                     LELVVRLLKPHRILEIGTFTGYSAICLATGLDEGGILDAVEKNDELATLIEEAIQRAN
                     LSDRIMLHYGDALEIIPKLNNSYELVYIDANKREYTRYFDLIIDKVPSGGIILADNVL
                     WDGKVYQDNIPIDTQTQEILKFNKRVREDSRVENFILPLRDGINIIRKL"
     gene            complement(10942..12144)
                     /locus_tag="P5293_02725"
     CDS             complement(10942..12144)
                     /locus_tag="P5293_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRR48849.1"
                     /translation="MRFAYKHINSPVAYCALTIGAGTRDEDPDVNGVAHFTEHMIFKG
                     TNHRSSVQINNLLERCGGELNAFTTKEETVVHATVLKEDLSKAIGLLFDIVFNSTFPE
                     EEFTKEKNVILEEIKSYKDSPSELIYDDFESYLFGEHPLSRPILGTTSTVKRINVGDI
                     KNFTSKFYLPGNMTFSIVSELPEERCLKLVGHYFGLDKAKKMESNRWTNCDCFLSINT
                     DKVKTISKKTHQIHCIVGSPAFSLYNRERIALSMLTNILGGPAINSRFNQILRERNGL
                     VYSVEASYTPYMETGIFTVYFGTDKSKLDKALKLVKHELERMRSSMLSATAVKSAKKQ
                     LLGQLAISMDNPENLCLSMGKSLLAYNKVETIDYIREKIEETTAEELQQIASEIFSEE
                     KMKVLIYK"
     gene            complement(12147..12995)
                     /locus_tag="P5293_02730"
     CDS             complement(12147..12995)
                     /locus_tag="P5293_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48850.1"
                     /translation="MRRLILLIFSLFFFTFASAERPVKDVKNNFRNFGSRTTKIVLSD
                     DGIIDAYIRDAAIKEWKISPYEFCTPQEYEVIKEDTSFFFLLRTDGIFSKEYEPSIEF
                     LSLIKGGPKFKRGLFMSAELISLPFQAKDDDSGEIIPFIPAYLQIIQDYIMTVQKNIL
                     ASISGNVVLGDLAGINGKTLLFKEEDIAFPAEKDEIKWIFRGRAEVVSQDVIESAISE
                     ISQDVAIGLVISPLRAHKGGYYYKLVIDPAEGKLYYFKKNRINEKLPVGFSRNDINKL
                     SAPYKR"
     gene            13049..14680
                     /gene="dnaX"
                     /locus_tag="P5293_02735"
     CDS             13049..14680
                     /gene="dnaX"
                     /locus_tag="P5293_02735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRR48851.1"
                     /translation="MERFIVSARKYRPNSFDTLIGQENIARTLKNSILRKQLAHAYLF
                     CGPRGVGKTTTARIFAKTINCSNPTADMNPCGECESCISFAEGRSYSIHELDAASNNS
                     VDDIRQLNDLVRIPPQIGKYSVYIIDEVHMLSQAAFNAFLKTLEEPPAHAIFILATTE
                     KHKILPTILSRCQTFDFNRISVEDIVKNLKIIAQQEKVDIDLESLHIIAQKADGAMRD
                     ALTLFDQTVAFCGNKVAYEQVISTLNVLDYDYYLRLTDCFLNSQHSEALLIFDEILSK
                     GFNALHFIGGLSSHFRNLLLCKDASTLKLLDLAPSVAEKYREQSTKCSISFLYTALNI
                     TTSCEAGYKLSGNQRLHIEFALIKLCRISATDKTAETVTEAAVSSKNNSSENETKQQK
                     HATTSIKNLYKSAEKEISTQEELRSQELGVDEFDMEKLLLIWEQMAESEKDFPNLANT
                     LRHSKPTISDNYKIHFTVSNNAQKEWIEKKCLPRLNVFLQKSLNNKMINLCVDVVSED
                     KITKKPYLPEEKASFLLKNNPELGELQKDLKLELK"
     gene            14699..15589
                     /gene="lptB"
                     /locus_tag="P5293_02740"
     CDS             14699..15589
                     /gene="lptB"
                     /locus_tag="P5293_02740"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRR48852.1"
                     /translation="MRLHCSNLVKKYGTRTVVKNVSIEVEQGEIVGLLGPNGAGKTTT
                     FYMITGLIKPNAGRIFLDEEEITDLPMYKRAKKGLGYLAQEASVFRKMSVENNILSVM
                     EFSNMTKKERHKRLEELLDEFGLQGVRKSMGIQLSGGERRRCEIARALAIDPKFILLD
                     EPFAGVDPIAVEDIQHIIANLKTKNIGIIITDHNVHETLAITDRAYLLYEGSILESGT
                     AEVLANNPEVRKKYLGENFELRKAVLKEKKNAEGTTNRSENKIHKPKTAESKDLKSIP
                     ENGADIQSYSDKANDLERGK"
     gene            complement(15602..16195)
                     /locus_tag="P5293_02745"
     CDS             complement(15602..16195)
                     /locus_tag="P5293_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRR48853.1"
                     /translation="MKKMLSIFTMLAIVANMSAQGVQKEAKPAMKLGMEKGYLRANPH
                     DLAPKFTEEQKKQVADFKLSRDKQLIQINNLLNEKRAQLKTLQQADKPDMKKINAKID
                     EITDLQNQKMKLNATFKNNVRSILTEEQRVRMDMMREHQRNNQGRPQRQIQIRTDMKP
                     MMTGHGQNRHDALCPGCPGCKESSACKDSVKVIKVKM"
BASE COUNT         5353 a         3035 c         3021 g         5052 t
ORIGIN      
        1 gacaaaatgc cccattaagg gacaaaaaca ttgtataggt tgccaatata tttttgacta
       61 tttttgtatg aagaatcatg aacataccta aatatataaa cgaaaagcat aacattgtta
      121 agcttgtctc acttaccgct ctttttgcac tgatttttat caacatctac aaacctttca
      181 gttcagaaag ctggtatgaa gtaagtgatt ttatgttttt tgtttactca agcctgataa
      241 tcctaacagg agttctggtt gtaattatca gcagaattat catgtacttc tacaaccgta
      301 aacatgaaat ctcatattcg ctttatgtct tttgggtaat cctggaaatt atattcatgt
      361 ctctttttta cacaatctat actttgagcg tcaaggatgg agctgatgtt atggagacat
      421 tcaaaaacag cataataaat acctctttgg tattgttgct tccatatgca actttatgga
      481 tttatatggg ttggaaggaa gctcaaaaaa aactgcaaaa aatagaaatg gatgaagaag
      541 atataaaccc attgaaaaca atagccttta aagacgaaaa gggacaagta atgctctcaa
      601 ttcctctgca ggaccttctt tacgtagaat caagcaataa ttatgttgag atccattata
      661 cttccaatgg caagaacaaa aaatatcttt taagaaatac acttaaaaat atagagaacc
      721 tgttggaaaa tacttctgta ggacgctgtc acaggtcata tcttgtcaac tttggcagag
      781 tcaaggtaat gcgcagagaa aaaggcggac tctttctgga gctggatgct gagggagtta
      841 tggatatacc tgtatcaaag agctttcaag ataaggttgg cgagaaattt gtctcaaatt
      901 cattctgaga taccttaaaa tcctaattaa tgagtattga cagttgatgc aagagggata
      961 cctttgcagc tgtcatgaat attgtatccg gaaaactgcg aattatattt gtctgcaatc
     1021 tgatttttgg aatgactgcc ttacctgctt ttagccagat aaaaattgat tctgtgattg
     1081 taacaggtta tcttaagggc ggaatcctcg aagagcagtt agtcccaata tcaaaaatta
     1141 atttgcagaa actcgaaaac gaaaacgtaa ctgctctcaa gggactatca ggatctgcac
     1201 ctaattttta tatacctgac tatgggtcca gaatgacctc ttctgtatat ttaagaggta
     1261 ttggtgcaag aattgacaat cctgtcatcg gactttatgt ggatggcatt ccatacctca
     1321 acaaaaacaa ttttgacttc gacttttttg atattcgttc agtagacatt cttagaggtc
     1381 cccagagtag tctctttggg agaaacacca tcggaggagt tgtaaatatt acaactctct
     1441 cacctttcgc ttatcaaggt acaaaagctt caattggcta tggcaatggt aacagtttct
     1501 caatacaggc ctctcaatac tctaaatgca atgatagatt aggcatttct gccggagctt
     1561 attataacag ttcagatgga ttctacacca acgatttttc acacaatgac ttggactggt
     1621 ataagggagg aggtgttcgt ttaaggcttc agtggatcaa atctgcagac ttaagttttg
     1681 acaactcttt tacaattggg tatctcgaac aaggaggatt tccatatggc aaagtagaca
     1741 cactcacatg gtctgcctcc aatccaaact ataacgacaa aagtggatat aaacgattta
     1801 ctgtcagcga aggagtttca ttcaaaaaaa atctggacaa atatattctg accggtataa
     1861 tatcatatca atatacagat gacaagatga acatggatca ggattatact ccggattctt
     1921 atttctcact tatacaaaat caaaaagaac atactattta tcaggatatt gtattaaggt
     1981 caaaagaaga taatcgttgg aaatggctga atggagcatc ttgcttcttt aaatatcttg
     2041 acatggatgc ccctgtgaca tttaaggaag acggtattga caggttaatc cttgcaaatg
     2101 caaataaagg attgtctttg atgttcccgg gagaagagat taaaattaaa gaggaaaact
     2161 ttgtaattaa cagctttttc aaaatcccaa cttatggtgt ggcactttac catcattcct
     2221 ttttcaggta tggcaaatgg ctgtttgaag ctggtttaag actggattgt gaaggctcac
     2281 atcttagata tgataatgga gctgatattc actatctatt taccatgaca atgtctgaat
     2341 atagagaaat tgtaacaaaa ttaaaaggta atgaaaatct actctttata gaaattcttc
     2401 caagagttgc agcctcatac aatacaggta attataaaat tttctcttct ttagcaagag
     2461 gttacaaggc tggaggatat aatactcaac ttttttccga tatacttcaa aaccagatga
     2521 tggaggatat gatgaacgat cttggagtac atctgaccaa ttctgcatat tccgcttaca
     2581 acattgcaga tgtaattaca tataaaccag aatattgctt ggattttgag ttaggagtca
     2641 actactcttc aaacaccggc tttaacttta atcttctatt ttttgatatt atctgtacca
     2701 accagcaact tacaatattc cctgaaagga acagtaccgg aagaatgatg actaacgcag
     2761 gaaagacaag aagttatggc gttgaaggaa gcatctccta taattggaaa aatctgaaat
     2821 tatatggtga ttacggatat actgatgcac gctttataaa atacaataat ggccgtaatg
     2881 attttagcgg caaatatgta ccttatgctc cgcaacaaac agtctcagcc ggtgtagatt
     2941 ataccattct cgcaaaaaat aatcagctgc ttccagataa gattgtaata catgcagatg
     3001 ttaaaggctt tggaaaaata tactggaacg agaccaatac ccttgctcaa aatttctata
     3061 cccttcttaa cggctcaatt tctttgcaaa aaaagccttt tactcttgaa ttgtggggca
     3121 agaatctttt atcctctgac tacagtacgt tttattttct ctccatggga aactcttttc
     3181 ttcaaaaagg gaagcctctg caatatggcc taaaatttaa aattaatata taacgtaata
     3241 aatatgaaaa catttaagac aatcaaattt tctgtaatta ttgcatttgc agttgtttca
     3301 ttttttgcat ccagttgcga aaaagatgac aatgagccaa tagtcattac tgaagatatt
     3361 accttctccg gaagaatgac agttgaccag cttgatggaa caaactatac tgttaacgga
     3421 gtccaaatta aaattgagcc tgcaacttct ggaaacatac tgacaataga aatgttgcaa
     3481 gtatcattct cgccaaaaat gccggtaaaa ctggatatga agatacagga agtaatgtat
     3541 tatagttcaa gtaaaaagat catcatctca ggaaacaata ttattccgat agcaatggga
     3601 ggagagttcc cggcatatac tattacaaat ctgtcaggtg aaatagaaaa cggtagtatt
     3661 accttgtcaa tgatgtgtgg aaattatccg ttgacttaca gtggtacatt gcagttatag
     3721 agtttcaata tctatatacc catgagtaac ggcataaatt gtcaatcctg aaactgactt
     3781 gatgttcagt ttgtcagtta tatttctcct gtgagttata actgttgtta tactgatatt
     3841 cagcctgtca gcaatttcct tgttcataaa tccctttacg accaacgtaa gcacctcagc
     3901 ttctctgtta gaaagcagcc tgggtgcttt gttctgaaca ggggcttcct gaacatgata
     3961 accattgctg tgggcatttt gatgaatact cagaattgac ttgataagag actcttcagc
     4021 ttgtgttacg ttgataattc ttattgtgtc gggaatttgg caatcttcat ctttagccgt
     4081 aagaactata gtttttttat ttctctctgt gaaaaaaact tgattttgga taaaaattcc
     4141 agcagagaca aaataatgga aaaattcttc cggccggtct tcaataaatg tattaatatc
     4201 ggtgtaaatg ctcaattggg caaatggcac aacttcgttt agaatagttc gaagtcccac
     4261 acaagtaact atattttcat caataaccgc taaacgcaga ttcccatgtg ttcccacgta
     4321 tttgtttgct ttaaaacttt tgcaaaggta attaattcaa aaaagatgac taaaaaacct
     4381 gcaataattg tagtaatcaa aaaataattt tgcaaataaa aaattttgcc tatatttgat
     4441 accgtattca tatcaaaata atatcacatc atgaaaaaag aaaaaattat aaaagcgcct
     4501 aacggctatt taatgtttgc agttttgtta gttatggtag cattcactat ttacttcctg
     4561 gtaaataatt tatatctgat tggaataata atgttggtta ttgatatatt tatgcttcca
     4621 ggtttcttcg ttatcaaccc caacagttcc aaagtactgt tgttgttcgg agcatatcgt
     4681 ggcacaataa aagtaaatgg gttttattgg gttatacctt tttacacaag atttggagtt
     4741 tctttgaaag caagaaattt cgagagtgaa aggttgaaag ttaatgacaa acttggaaat
     4801 ccaataatga tcagttcaat attggtgtgg aaagttgacg acacttacaa agctatgttt
     4861 gaggtagatg attatgaggc tttcgtcaag gttcagactg atgccgctgt ccgcaagctt
     4921 gccggatctt atccctatga tcagtttgaa gatgaaaaag catctgttac actaacctct
     4981 aactttgaag aagtaagcaa gagtctggaa actgaaatct ctgaacgtct gaaactggca
     5041 ggtataacag ttatagaagc aagaatcgga tatcttgcat attctcagga aattgcacag
     5101 gcaatgctta agagacaaca ggcaactgct attattgcag caagaagtaa gatggttgaa
     5161 ggagccgtag gcatggtgga aggtgcactc agactgctct ccagcaagaa agttgttgaa
     5221 cttgacgagg acaaaaaagc cacaatggtt agtaatttgt tagttgtttt gtgtggcgat
     5281 aaagagacct cgcctgttat taatacagga acattatatc agtaacaaat cttcagatgg
     5341 gagagaaaaa atcatttgta ttgagaattg atccagaaac aatgaaggca attgagaagt
     5401 gggctgctga tgaattcagg agtataaatg gacagttgga atggataatc aacaaaagcc
     5461 tcaaagaggc cggcaggctt aaggaggata aaaaagaatc tgagacgtaa tatcatcatt
     5521 catcatattt atgctaactt tggccactga tagagttgtc attctatagc tctatagcat
     5581 taaatttgat aattgaaaac atatatgtgg agaaaatggc ttctgattat cggaatactt
     5641 ttgacgtcgc aactttcaat tgccggtaat cctgaagata ctatccctac ttccaatcgt
     5701 tatattcatc agctggaaac agaagtccgt caagcatata tattcccttc aacttctttt
     5761 ctggaaggag aaaacaataa atcaaaagca ctgcgcagcg ctttttctgc tcatctacgc
     5821 tatgcttttt cattccctcc attatcatat atagatcgta tttatggaag tccctatcaa
     5881 ggtataggtg tggcatatta caattttggc gatcggcatg aaataggcaa tcctatagct
     5941 gtgtatctgt tacagggagg acgactggcc gattttagct ctcgactttc tttaaactac
     6001 gaatggaatt ttggtatctc tgcagggtgg cacccttatg atgttaacag caatcctaat
     6061 aatacagtta tcggttcaaa agtaaatgca tatctcaata caagtgctta cttaaatttc
     6121 acgctgtcgc cacaatttga cttgacatcc ggcatttcgc tgactcattt ctccaacgga
     6181 aataccgact ttccaaatgc aggccttaat acaatcggta taaagatggg gttaacttac
     6241 aatttcaaga agaataaggc aatacaatta aataatactc cacctgttct catcagtgat
     6301 tttccgcgtc actttagtta cgatctgata ttttttggtt catggcgccg taaaggtatt
     6361 gtatataatg acatccctct cgcctcccct tacacttatg aagtgtttgg atttaatttc
     6421 acctcttttt acaacataag ataccgattc agagcaggta tatctttaga tggagtctat
     6481 gacagcagtg ccaacattta tacagaagag tataatgtga ctgcagatgg acatgatccc
     6541 agttttactt tttataagcc tccattaaaa aagcaactat ctttgggact ctctgctcgt
     6601 gccgaatata ttatgcccta tttttcaatt aatatgggat tgggaaccaa tattatccat
     6661 ggcggaggag acctgagagg actttatcag atagtggcac tgaaaataga gacctcacgc
     6721 aattcattta tccatattgg ttacaatctg catgatttca aagagcctaa ttatctgatg
     6781 ataggatttg gttatcgctt tcacaataaa tatcctttta tttatcactc cttttagttt
     6841 tttgtatatg atatcgtggc ctaaatcttc atgttcttta acaggccttt ggtgacagaa
     6901 gctattctaa aactgtcaca tgctttctga attgttttgt tatgaacaaa ttctgagaag
     6961 gatcggtttt caagataccg aactgtttta tcataaaatt tgacaagtcc attgcttaaa
     7021 gcccacgcta tccccatatc gacatagtat tgcccctgac gaacctgaga aagatgcttt
     7081 aaagttgcgt caatatgagt agcatccatt aaataatcca tcataaagat tacaaaaaaa
     7141 cgctttgtaa actctccgtc atcttccttt agaggtaaaa aatgactaaa tgcctcatca
     7201 ggactttttt taaaaatctt taaggctgaa acaacagaat ccacatgtgc ccagttgtca
     7261 aatttataaa tcaacggatc aagataagtg aaaagagttt caatggaaat attttttaat
     7321 tctcccaaaa tcagcccata aagaataata tgttcatatg attggtcctc tgatttcagt
     7381 gcgtttttga gataaacctc aatttcattg tccgttgaaa gttcttttgc caactttttc
     7441 agtgaaggca ttcttacacc aagtaattcg tatcttgtag gaactatctt ttggttaaaa
     7501 aatctgtatt tctcctcagc aagtgctttt aattgaaaat caatattctc ctttatgctc
     7561 atactttatt acctatgaca gtaaaaacat caggtcataa aaatcacggc ctttagctcg
     7621 ggctaacatt acagcaattt tcatactgca caatacacca tcggaaggaa cgggaaatgg
     7681 gaaaaagaat ccgcgaccct tgatatttgt aataatatgt ccgctcaacc ccaaattgag
     7741 taatagttct ggaaaatgaa tattacgccg taaagccgac cgataaaagc catctttctg
     7801 atgcttggag tagaagaaag gtaatccaga atcatcaact gcaaatattc tttcagtatg
     7861 cgcttatcga aactcgaatt tccacgaatt tgagaaggaa aaaaatttct tatctgttca
     7921 atctttatca taagtggctt aacattttta gagaaaagga aaatccctga aatatagata
     7981 tctcagagat tttacaatta tggagtcttt ttgaagtggt gtcaccggga atcgaaccag
     8041 ggacacaagg attttcagtc ctttgctcta ccaactgagc tatgacacca ttctttaact
     8101 ttgggaatgc aaaattagta ctaattttct tttatacaaa attttcgtac tgctttgtcg
     8161 gaatgaggcg actcgaacgc ccgaccccta cgtcccgaac gtagtgcgct accaactgcg
     8221 ctacattccg atatttgtag tatatttgtg acaaatatat acaaaaatat ggcaacaact
     8281 gcggatttta aaaacggttt atgtattgat tttaatggaa aaccatgcag cattatttgg
     8341 tttcaacatg ttaaaccagg aaagggaggt gcatttgtga aggttaaaat gagaaacctt
     8401 gaaaatggta aaatacttga aaacacatat actgccggtg aaagaattga gatccttaat
     8461 gttgagagaa gaccttatca atttttatac aaagacgaca tggggttcaa ttttatgcat
     8521 accgagacat ttgagcaaat cacacttgat ccagaaatgg ttgaaaatgc cgatctaatg
     8581 aaagagggtc aatatgtgga gatgatgttt ctggctgacc aggagagggc gctcacttgt
     8641 gaactgcctc cgtttgttga attagaagta acctacactg aacccgccgt taagggggat
     8701 actgcatcca caaatgcttt gaaggcggct actcttgaaa caggtgctga aattatggtg
     8761 ccacttttta taaaccaggg cgaaaggata aaagttgaca caagggatag aagttactac
     8821 gaacgctgta agtaaaaaga aaaaaatgaa acataaatcc atttttttgc cttttctttt
     8881 tctgctgatc atatcctgtt caggagaaaa gagttaccag ttgcctgcta ttgcacatat
     8941 taacaatgaa atatcatcag gtaattttac tgaagcatcc aaattgataa aaatcagatt
     9001 gatgagcgat actctgcctg aaattgaaag gtggaatctc aattttgagc tggaaaagat
     9061 ggatcggatc agatacgatt tcagggcaag tgactcttca gtaatcagtt atattaagag
     9121 atattatccc aacgtcacaa aaagtgaaat tgaacattgg gaacagacta atgctcttga
     9181 aaacatgatc attgatggca aaaaatgtta tttcagatct gcagcaagaa accttttcag
     9241 aatagactcc attgctgcaa aacatttcgt cgctgctgaa ggtcaagcag gagattcgtt
     9301 aacacgattc cttgctaaac acattcctca attgatttct gaacgccaaa atgttaatgg
     9361 cacttatttc aagcctgtaa caatgaatgt taaatataca ttatctgtca aacccaacga
     9421 agtgcccgat ggggaaattg tcagggtatg gatgccttat ccaagaggcg atgtcagaag
     9481 tcaatccaat attcgtttga tttcaacatc acagcctgat ttcttaattt ctcctgacaa
     9541 atatttgcat aaatcaattt atatggagaa aaaggctgtc aaggattctg ccactatttt
     9601 ctcctacaac ttagaatata catgctatag tcagcatttt aatttcgacc caaacaatat
     9661 taagccttat aatattgaag attctatcta caaaaaatat acatcggaga tacctccaca
     9721 tatagttttc agcaacaaaa tcaaggatac tgttgctaaa atagtcggca gtgaaaccaa
     9781 cccatacaaa aaaatcaagc tgatatatga gtggatagac agtaattttc catgggcaag
     9841 tgcaagagaa tattccacaa ttgaaaacat tccggaatat gtacttgcaa atcgccatgg
     9901 agattgtggg caagtatcgc tgcttctgat cacgatggca cgatgtgcag gggttcctgc
     9961 caaatggcag tcaggatgga tgatgcaccc aggtaatctc aatctgcacg actgggctga
    10021 aatttactat gatggtatag gctgggttcc tgtggatcag tcttttggaa gagttaaaag
    10081 tgcattgggt aatgatgaag tctattattt ctatacaaaa gggatagatg catatcgaat
    10141 gattgtcaat caggatattt cggcaccttt attccctgca aaaatctatc cgaggagtga
    10201 aactgttgac ttccaacgcg gtgaagttga gtggaaaggt ggcaatttgt attttgacag
    10261 gtggaactac gatatggaag tgaagtatca ttgatactta agacatcact cataattttc
    10321 taatgatatt tatcccgtca cgtagtggaa ggataaaatt ttccacgcga ctatcttccc
    10381 ggactctttt attaaacttc agaatttcct gggtctgggt atcgataggg atattgtcct
    10441 gatagacttt tccatcccag agcacattgt ctgcaagaat aatccctcct gaaggaacct
    10501 tatctattat caagtcaaaa tatctggtgt attccctttt gtttgcatct atataaacaa
    10561 gctcgtagct gttattgagt tttggaataa tttccaacgc atctccataa tgaagcatta
    10621 ttctgtctga aaggttagct ctctgaattg cttcctctat tagtgtagca agctcgtcat
    10681 ttttttcgac agcatcaaga atacctcctt cgtcaagtcc cgtagcaagg caaatggccg
    10741 aatatccggt aaatgtacct atttccaata ttctatgggg tttaagcaac ctcacaacaa
    10801 gctcaagaaa cctaccctgg actgcacctg tcagcattct tgcatgactg gttctcagat
    10861 tggtttcacg ctctaaccag acaagagcat cgcttaaggg tgatgaattg tcaaatatat
    10921 atttttcctc ttttgtcaca attatttgta aattaatact ttcatttttt cttccgaaaa
    10981 aatttcactt gctatctgct gcaattcttc tgcagttgtc tcctcaattt tttctctaat
    11041 ataatcaatc gtttcaacct tgttatatgc aagaagagac ttacccattg agagacatag
    11101 attttcggga ttgtccatgg aaatggccag ttgacccagc aattgcttct tagcagactt
    11161 aacagctgtt gctgaaagca ttgaagatct cattctttct aattcatgtt tgacaagttt
    11221 taaagccttg tcaagtttgc ttttgtcagt gccgaaataa acagtaaaaa taccggtttc
    11281 catataggga gtgtacgaag cttctactga ataaactaga ccatttcttt ctcgcaaaat
    11341 ttggttgaat cttgaattga tagccggacc tccaagaata tttgtcaaca ttgaaagagc
    11401 aattcgttcc cgattgtaaa ggctaaaggc aggagaacca acaatacagt gaatctgatg
    11461 agtctttttt gaaattgttt tgactttatc tgtattaatt gataaaaaac aatcacaatt
    11521 agtccatctg ttagattcca tttttttagc tttatcaaga ccaaaatagt gcccgactaa
    11581 cttaaggcat ctctcttccg gcaactctga tacaattgaa aaagtcatat tacccggaag
    11641 ataaaatttt gatgtgaagt ttttaatatc gccaacatta atcctcttga cagtggaagt
    11701 tgttcccaaa ataggtctgg aaagggggtg ctcaccaaaa agataactct caaaatcgtc
    11761 atatatcaac tctgaaggag aatccttata tgactttatc tcttccaaaa tcacattttt
    11821 ttcttttgta aactcttctt caggaaaggt tgaattgaaa actatgtcaa ataaaagtcc
    11881 aatggctttt gaaagatctt ctttcaagac tgtagcatga actactgttt cttctttagt
    11941 ggtgaatgcg tttaactctc ctccacatct ttcgagaagg ttgttgatct gtaccgaact
    12001 tctatggttg gtacctttaa agatcatatg ttcagtaaaa tgggcaactc cattgacgtc
    12061 tggatcctcg tctcttgtac cggcaccaat agttaaagca caatacgcta ccggagagtt
    12121 tatgtgctta tatgcaaacc tcataactat cttttatatg gggcagataa tttgttaata
    12181 tcatttcggg aaaatccaac aggtaatttt tcattaatcc tgtttttttt gaaataatat
    12241 aatttaccct cagcaggatc tatgactaat ttatagtaat accctccctt atgcgctctc
    12301 agaggagaaa taaccagacc gattgcaaca tcctgggata tttcagaaat tgcagattct
    12361 ataacatcct ggctaaccac ctctgctcta cccctgaata tccactttat ctcgtctttt
    12421 tcagcaggaa aggcaatatc ttcttcctta aacaataatg tctttccatt aattcctgcg
    12481 agatccccaa gaaccacatt gccactaatc gatgccaaaa tgttcttctg aacagtcata
    12541 atataatctt gaattatttg aagatatgca ggaatgaaag ggataatttc tccagaatca
    12601 tcgtcttttg cctgaaaagg taatgaaatc aattctgcac tcataaacag acctctttta
    12661 aattttggtc cgcctttaat gagcgaaaga aactctatgg atggctcata ctcttttgaa
    12721 aaaattccat ctgtccttag cagaaaaaag aatgatgtgt cctctttgat cacttcatat
    12781 tcttgaggcg tacagaattc ataaggagat atcttccact cttttatagc agcatcccta
    12841 atataagcat ctataatgcc atcatcagac aaaacaatct ttgtcgtcct ggatccaaag
    12901 ttgcgaaagt tgtttttgac atcttttaca ggtctctctg cagatgcaaa tgtgaagaaa
    12961 aacagcgaaa aaataagcag aattaatcgt ctcatattgt attaccgtaa tacacaaaat
    13021 tagataaaaa tttgtacttt tgcccggtat ggaaagattt attgtatcag ccagaaaata
    13081 cagaccgaac agttttgaca ctctaatcgg tcaggaaaat attgcaagga ctctcaagaa
    13141 ttcaatacta cgcaagcagt tggctcatgc ctatcttttc tgtggcccaa gaggtgttgg
    13201 gaaaactaca acagctcgca tttttgcaaa aaccatcaat tgttcaaacc ctacagctga
    13261 tatgaatcca tgtggagaat gcgaatcctg tatctctttt gcagaaggac ggtcatactc
    13321 aattcatgaa ctcgatgcag cttctaacaa ttcagtagat gatatccgac agttaaacga
    13381 tcttgtgaga attccgccac agatagggaa atacagcgtc tatatcattg acgaggtgca
    13441 tatgctctct caggcagctt tcaatgcttt cctcaaaacc cttgaagaac cgcccgctca
    13501 tgcaattttc atactcgcaa ccacagaaaa acataagatt cttccaacaa ttctttccag
    13561 atgtcaaaca tttgatttta acagaatatc agtcgaagat attgttaaaa acctcaaaat
    13621 tattgcccag caggaaaaag tcgacataga tctggagtcc ttacatatta ttgcacaaaa
    13681 ggcagatgga gctatgagag atgcacttac tcttttcgat cagactgttg cattttgtgg
    13741 aaacaaagta gcttatgagc aggtcatttc tactttaaat gtgcttgact acgattatta
    13801 tttgagactg actgattgtt ttctcaattc tcaacactct gaagcactgt tgatttttga
    13861 tgaaatactt tcaaagggtt ttaatgctct gcactttata ggaggattaa gttcgcattt
    13921 tagaaattta ctcctttgca aagatgcaag tacgctcaaa ttattggatc tggcaccaag
    13981 tgttgctgaa aaatacagag aacaatctac aaaatgcagt ataagctttc tttatactgc
    14041 acttaacatc actacatctt gtgaggcggg atataaactc agcggaaatc aaaggctgca
    14101 tatagaattt gctcttatca agctctgtcg aatttcagcc actgacaaaa cagccgaaac
    14161 tgttacagaa gctgctgtaa gttcaaagaa caactcctcc gaaaatgaaa caaagcagca
    14221 gaaacatgcg acaacttcaa ttaaaaattt gtacaagtca gccgaaaaag agatttctac
    14281 gcaggaagaa ctaagatcac aggaactcgg agttgatgaa tttgatatgg agaaactatt
    14341 gctcatatgg gaacagatgg cagaatccga aaaagatttt cccaatcttg ccaatacctt
    14401 gaggcattct aagccaacta tttctgataa ctataagatt cattttactg taagcaacaa
    14461 cgcacaaaag gagtggatag aaaagaaatg tcttccaaga cttaatgtct tccttcaaaa
    14521 aagcctcaat aataaaatga tcaatctttg tgtagatgta gtctctgaag ataaaatcac
    14581 aaaaaaacca tatcttcctg aagaaaaggc aagttttttg ctgaagaaca atcctgaact
    14641 gggagagttg cagaaagacc ttaaacttga gcttaaatag caacgaaaat aatttataat
    14701 gcgtttacat tgcagtaatc ttgttaaaaa atacggaacc cgcacagtag ttaaaaacgt
    14761 ttctatcgaa gtagagcaag gagaaattgt gggtttactt gggccaaacg gagccggaaa
    14821 gacaacaact ttctacatga taacaggtct tattaaacct aacgccggaa ggattttcct
    14881 tgatgaagaa gagattactg atcttcctat gtacaaaagg gctaagaaag ggttgggtta
    14941 tctggctcaa gaggcttctg tatttagaaa aatgagcgtt gaaaacaata tactctctgt
    15001 tatggaattt tcaaacatga caaaaaagga gagacacaaa cgtcttgaag aacttttgga
    15061 cgaatttgga cttcagggag ttagaaaaag tatgggaatc caactgtcag gaggtgaaag
    15121 aagaagatgt gaaattgcac gtgcactagc tattgatcca aaatttatac tgctcgatga
    15181 accgtttgca ggagtagatc caattgccgt tgaagatatt cagcacatta tagcaaatct
    15241 taaaacaaaa aacatcggca ttatcattac tgaccacaat gttcatgaga cccttgccat
    15301 tactgacagg gcatatcttc tttatgaagg aagtattttg gaaagtggga cggccgaagt
    15361 gcttgcaaac aatcccgaag tcagaaaaaa atatcttggt gagaatttcg agctcagaaa
    15421 agctgttctt aaggagaaga aaaatgccga aggcaccaca aacaggtcag aaaataaaat
    15481 ccataaaccc aaaaccgctg aatcgaaaga cctgaaatct atacctgaaa atggtgccga
    15541 catccaaagc tactctgaca aggcaaatga cctcgaaaga ggaaaatgat aaactattta
    15601 actacatctt aactttaatt actttgacag aatccttgca agctgaagat tctttgcaac
    15661 caggacatcc cggacataaa gcgtcatgtc tgttttgccc atgccctgtc atcatcggtt
    15721 tcatgtcagt acgtatttga atctgacgct gtggccttcc ctgattattc ctctgatgtt
    15781 ctcgcatcat gtccattctg actctctgct cctctgtaag aattgacctg acattgttct
    15841 tgaaagttgc attaagtttc attttctgat tttgaagatc tgtaatctcg tcaatttttg
    15901 cattaatttt cttcatatca ggtttatctg cctgttgaag tgttttaagt tgagcccttt
    15961 tttcattgag aagattattg atctgaataa gctgtttatc gcgggaaagc ttgaagtctg
    16021 caacttgttt cttttgttct tctgtaaatt taggagctaa atcatgagga tttgccctca
    16081 gatagccctt ctccataccc agcttcatgg ctggtttagc ctctttttga actccctgag
    16141 ctgacatgtt tgcaactatt gcaagcatgg taaagatact aagcattttt ttcatattat
    16201 tggcaattta tattgtttgt aattaagact tcaagcctta taaaaggttt aattgttatg
    16261 aagaattgtt acctttgcca caaaataagg cattattaac ataacaatca atatggcaga
    16321 aaaattattc gctgagtttc cgcctgtttc cacccaacaa tgggaagagg tgatcgctaa
    16381 agaccttaaa ggcgctgact acgagaaaaa actcatatgg aagacattgg aaggatttcc
    16441 ggtgcgtcca tattaccgag c
//
LOCUS       DAOTQL010000024        15522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3664_length_15522_cov_5.439064, whole genome shotgun sequence.
ACCESSION   DAOTQL010000024 DAOTQL010000000
VERSION     DAOTQL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3664_length_15522_cov_5.439064"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..801)
                     /locus_tag="P5293_02750"
     CDS             complement(142..801)
                     /locus_tag="P5293_02750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRR48854.1"
                     /translation="MRNNSLRVIDLTHTIESTMSVFPGTSTPVITQESTIERDNYAEK
                     SLSFCSHTGTHIDAPSHIFENSCSLDKLTVDKFIGKAVVIDVSSCENELIGKELIDRN
                     IRVISKVEFVLFYSGWDIKWGRSEYFEGFPTLSPEAAKYLCNFKLKGVGFDVISADPV
                     ASSGLPNHKLLLGNGIIIVENLCNLDKLPNIPLTFITLPLKIAESDGAPARAVGIVSQ
                     F"
     gene            932..1696
                     /locus_tag="P5293_02755"
     CDS             932..1696
                     /locus_tag="P5293_02755"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HRR48855.1"
                     /translation="MNPIIHINPKYLYLKDFILNIPAQFYEMGIPIHTGRNELRLVET
                     ENVKIVIKHFKRITEINRFIYANFRKSKGRRSFEHSMQLIEKGFASPEPVAYINLIKG
                     GILTDDYYICLYCDFRPFKELLSKPLAEAQKGLEEYAKYTYKLHKCGIFHKDYSTSNI
                     LFNDNGGNYEFSLIDDNRLLFRSYSKTRSMVTMVKRQDISVEQMGIIAGSYARAAGEN
                     EYSILNNMTRQVLIISKVFSVRRRIKKSLKAIKVKM"
     gene            1758..2786
                     /locus_tag="P5293_02760"
     CDS             1758..2786
                     /locus_tag="P5293_02760"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HRR48856.1"
                     /translation="MTNYLTNEKKIANLKGLIGKTPLLKIHYSYKGEERNLYAKAEFL
                     NFTGSIKDRMAYYIMWDAVLTGKLKPGMVVAEATSGNTGISIVAISRFLGHNVSIYMP
                     DWMSSERIELIKSFGANICLVSKEEGGFIGSIEMTNKLKRDDPDGVFLPLQFDNEINC
                     KAHYFSTGPEIYNQLNSQGIIPNAFIAGVGTGGTIMGTGLFLREAYPDIKVHPLEPAS
                     SPTLSTGHCVGKHRIQGISDEFIPSILDLDKLDEVIQVDDGDSIIMAQMLAKSFGLGV
                     GISSGANFLGAVELQNRHNSDYDVVTVFADDNKKYLSTDYSKEEPVKPNFLSKDIRLL
                     EMVAIPYY"
     gene            2800..5409
                     /locus_tag="P5293_02765"
     CDS             2800..5409
                     /locus_tag="P5293_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR48857.1"
                     /translation="MKKLFQNSVIVSILIVLFSTLNISTYAAVGGKQLDSTVFETWRT
                     CESIKIADDASWSIAEYKNKEDSSILVIKIDKINFRKEIKKGKNPQMFNNMESAVFFV
                     KDTLFTINGHEGTIQKIGITKSASVNDKSPFFTYKRGKTLFISNVKNGSTDSIKNVGT
                     SIFINPETLTATVNEGKSNIILIIRLGNKKGIDTTQIYKTDKFITDVSSNKNGDKLIF
                     FTCIDSLLKSPEVTMIKIKERSANKINLNESLLPEGMQFNIKKGIVFSQDDSYLKFEI
                     EPVKANHDKSNEKVSKPPFDLELWKWNDTLIPLQQKRGPKAFEKSMKCAFYPETNKFV
                     QLSTGKGVYLQIDDNSPYGIEFNERPYLYRSNWEDPTPRDIICIDVKTGERKILLKEF
                     KGSIAVSPRTPHLFIFEPENGEWSIIDTKSGAKTLISDLIDYPLQDEEFDRPQPAGSY
                     GQAGVSRDNQYFLVYDKYDVWALPTSGIGEPLCITNGYGRKHNIRFRILKIESDSDQN
                     KNRGVNLKETIFLESLNLNNMYSGYYKVAPGKDPVKLIEDACKFSFYKKLGKDNYIFK
                     KESFDEAPDFWLSSKDFQPVRRLTDLNEQICGYFIGKSKVITWSDGSGKEQKGVLYTP
                     ADYDSSKQYPVIVYFYETMTENTFLFYHPLPTESTINPILFVSRGYVVFMPDIHYEIG
                     WPGKSCVNTVVSGTEYLIKEGIADPERIGVQGHSWGGYQVAFLITQSDIFKCACSEAA
                     VTNMTSAYTGIRYGSGSPRMFMYECSQSRIGGNLWEKQNNYILNSPTFFLNRVTTPLL
                     SRHCDADEAVPYSQGLELFLGLRRLGKEVWMFNYEGVGHNIKPWEVKKDWTKRMDEYF
                     DYYLMDKEKPSWM"
     gene            complement(5412..6953)
                     /locus_tag="P5293_02770"
     CDS             complement(5412..6953)
                     /locus_tag="P5293_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR48858.1"
                     /translation="MNRLFICALLTIGIIVETSPLVWSQELVNYDPGEQMIRFDLKKG
                     TVYQMINRLSDITGYYFVYDSELINNDRKVRINPGDYKLKDLIREITGNNVDVKLQDK
                     YLLLFISKGNTEITSELKLKSSADSVFIVKGRLLDRITGEPVVYGTIDVACGKKTTVS
                     NQDGNFMLKLPDSLQSSVIRISHIGYLNRMISADVIAGKNIDIYLDQKIIPLQEIVVR
                     MVDPKKVLREMLKRRQDNYNSETAYLTSFFREGIENRHGFNLTEAVLKIYKTGIHSTL
                     ASEQVKILKMRRLGTPNYNDSLIVRLKSSIYSCQMLDIIKNLPDFLDPQYYNYYNYSH
                     TDITVLDERRVLVFFFEQKEEIKDPLFMGKLYIDAENYALLKATFQINPLYVEDAADI
                     LVLKQSRHIKAIPQSAIYEVSYAPVDGKYYLNHVRADLTLKMKKRGSLFSSNEHVWFE
                     MANCETNTSGVKRFANEEIVSNKNFLSEVSYSYDEAFWGNFNTLLPEKSLKELIRKYN
                     FDIGN"
     gene            complement(6946..7797)
                     /locus_tag="P5293_02775"
     CDS             complement(6946..7797)
                     /locus_tag="P5293_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRR48859.1"
                     /translation="MNSKKNSEMTNLAWSKLYDRLNNEGLLYTENSKGRRGKMLHNIT
                     LRWALIGMIVVLVSVYLGIDRYNNVGKMLSYSNNDNSTLVKTLEDGSIVFLENGSEIT
                     FPAKFGKVRKIYLRGNAFFEVVRNTETPFIVDAGMVEIKVLGTSFSVETENHSCPSLS
                     VRSGIVKITLKENGTNQKVVAGETAFVERHSLRIVPTLDLSKFAVYSERIHFKDEKLR
                     DVVKVVNKMKKSGDLTLTLADDEKLPERSLTATFIDRSTESIAQMICFALDLNYEIRG
                     DVIVIYE"
     gene            complement(7803..8342)
                     /locus_tag="P5293_02780"
     CDS             complement(7803..8342)
                     /locus_tag="P5293_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRR48860.1"
                     /translation="MLQDLLLVKRIKEGDIAAFEQLFKSSYMSLLYYSISITGSKEIS
                     EEIIQELFYYIWKERDKLQIMSSLKGYLYKSVKNRSIQYLHSKCFNMQFKSSSEVEMN
                     TGEDGESNMELKDLENVILKSMEMMPIRRAQIFRMHRFDKMSYSEIAEKLSLSIKTIE
                     AEIGKALKTLRREVKKYND"
     gene            complement(8390..9652)
                     /locus_tag="P5293_02785"
     CDS             complement(8390..9652)
                     /locus_tag="P5293_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRR48861.1"
                     /translation="MKKRIKILATLLSLLLPILTVTPALSQAEKGFYTVKGVVKDKTT
                     NKTLEYANIFVSGTNIGTVSNKDGEFSIKIKNELNAKTIEVSRIGYSSEVIAVKGVNI
                     DNLVVYLSPRDNVLTEITILGMDPEMIIREAISRIGDNFSNQENLLSGFYRETVRKRS
                     TYVNVAEAIVEIFKTKYTDNYEGDRVQVYKGRRIVSPKANDTLVVKLLGGPNLSIYLD
                     VVKNPDLMLNPASLGDYTYKMEMPVMIDDRQNYVVSFAPQVVLPYPLYYGKYYIDRES
                     LTFSRIEFSLSMDDRNKVTQMILKKKPFSLRFKPEEVSYLVTYKRLGDKSYLNYVKSE
                     IQFKCDWKKKLFSTNYAIVAETVITKVSNDIPLKFTKKYAFNDNYSLSDKVDDFYDEN
                     FWEDYNIIEPEQSLENAVNKLRKTINKK"
     gene            9805..10341
                     /locus_tag="P5293_02790"
     CDS             9805..10341
                     /locus_tag="P5293_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRR48862.1"
                     /translation="MDTNAEILVDKRTIDEIISRNAGKPGMLLTSMEEIQEANEYKFL
                     DQQSMEHLSYRTKVPLSQIYSVATFYSFFNLKPQGKHTIIVCRGTACHTKGSKVLLED
                     LNILPGCKDAFDNGETSFTTEDRQITVKTVACFGQCALAPVVAVDGMIYSNVTSEMIL
                     KLLQNLREDKKDENIKLC"
     gene            10319..13483
                     /locus_tag="P5293_02795"
     CDS             10319..13483
                     /locus_tag="P5293_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR48863.1"
                     /translation="MRISNYADLEEIRLKAATKILPDKPYIAVGMGTCGIGRGAEVLY
                     DSFQRIINERKLDIILKKTGCFGFCAEEPLVNIYIPGKPLVILHRLAEVDVIPVVSAI
                     QKGLMPFRNVLCRIEDWDFITGKFEFGKGLTEHPLWNEIPFFKWQKKIVLRNCGLIVP
                     DDIEEYIGVGGYFPLYSVLTGKSPQEVIDEVKLSKLRGRGGAGFPTGQKWELMAKVKA
                     DQKYVICNADEGDPGAFMNRNEIESDPFMLLEGMTIGAYAMDASKGVVYIRAEYPLAV
                     RRLKGAIEQAKSLGLLGENILGSGFNFDIDIVEGAGAFVCGEETALIKSIEGKAGRPV
                     PRPPYPAVKGLFDYPTNINNVETWCNIPVVMSKGASWFTETGTDKSAGTKVFSLVGKV
                     KNTGLVELPLGSTLETIVFRIGEGSGSQKRVKAVQTGGPSGGCIPVDYMKTPVDYESL
                     NALGAIMGSGGMVVMDQDNCMVDVARYFTEFSNSESCGKCTPCREGLAQTLEIIKKFT
                     SGKAQEEDIKTLEELGQVIKATALCGLGQTAPNPVLTTLRYFRDEYDHHMIEKKCEPG
                     ICQDLFTALCENSCPMHMNIPRYLQLLKENKLEDAFESSLRDNPLPSTIGRICHFHCQ
                     MKCRREGIDAPVSQGEIHRYISDTVYAKESYRKIYDRLISEKMPPTHKKIAIVGGGPA
                     GLTAAYYLVRLGHDVTIYDSESEAGGILRWGIPKYRLPLDILQREIDFIKELGVKFVM
                     NTCVGKDISLEQLEHDNHVVILAVGAQKDTELQIPGCNLNGVISGMDFLKEIAHEKKP
                     EIGHHVVIVGAGNVAIDAARTALRLGSEVTVVYRRLKADMPANKDEIKEAENEGVNFL
                     FLCNPEEILADANDKVRGVRLMKMACGEVDLSNRRKPVPTGEFFTLDCDNVIMAIGEE
                     VDAEFAKEYGINLKSNGNILVDHFTHETNHPKFYAIGDAITGPATAAEAMGIAKKAAE
                     VIDLKLMDKNRFHKLFREFEYSNEVPLKPEGGNRHYSVKLPLEERIGNFKEISLGYTP
                     AQAYAEAARCLRCDVRTTVKREEE"
     gene            13486..15258
                     /locus_tag="P5293_02800"
     CDS             13486..15258
                     /locus_tag="P5293_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRR48864.1"
                     /translation="MENNKVNITVDGREYQVPAGATILEACREIGIQIPSLCYMKDVS
                     HNASCSICVVEVKGAKTLVRSCISTVAEGMDIKTHSTLVDQARKINLELILANHPKDC
                     LICERNGNCELQDLTSIIGIKETPFLKTKTAAKVDDTSLSLLRTPEKCILCGRCVAVC
                     AEVQTVNAINFTGRGLKTKVSTYLDKGLGNVACTNCGQCALVCPTAAITERDESRNVF
                     NEIYNKDKFVVVQTAPAIRVGIGEAMGMEYGALVTGKMVAALRRVGFDKVFDTQFAAD
                     LTIMEEGYELIERIKNNGALPMVTSCSPGWIKFIEHFFPKSLDHLSTCKSPQQMFGAV
                     AKTYYAEKLNIDPRKIVVVSIMPCTAKKFECKRPEMKSAFKYWKDKINLNENEEFFDV
                     DYVLTTRELARMFKQSGIDFKNLPEEEFDEPLGISSGAGVIFGATGGVMEAAVRTAYE
                     VLTSKPLPNIDLKAVRGFEGIKEAELDINGMKLKVAVAHTLKNARTLLDQIEEGKSPY
                     AFIEVMTCPGGCLGGGGQSIPTTWEIRQKRADSIYREDSLKPIRKSHENPAIIAIYEE
                     FLEKPLGHHSHELLHTSYVERGIF"
BASE COUNT         5073 a         2901 c         2944 g         4604 t
ORIGIN      
        1 cagttggtta gagcacctga ctgttaatca gggggtccta ggttcaagtc ctagacgagg
       61 agcttaaaac taaagcactt caagctacct ttatatcagc aacagaaaaa cttctccgaa
      121 tttttctaaa gatcgtttca atcaaaactg tgatacaatc cctactgctc gcgcaggagc
      181 accgtcactt tcggcaattt tcaaaggaag agttataaaa gttaatggta tgtttggcaa
      241 cttgtcgaga ttacacaaat tttccacaat aataattcca tttccgagca gcagcttatg
      301 attaggcaaa cctgacgaag ctacgggatc ggctgaaatt acatcaaacc ccactccttt
      361 cagtttaaaa ttacaaagat atttggcagc ttcaggagat aatgtcggaa aaccttcaaa
      421 atattcgctc cttccccatt ttatatccca tccggaataa aataagacaa actcaacttt
      481 ggatatgact cttatatttc tatcaatcaa ttccttgcct atcaattcgt tctcacatga
      541 actaacatca attactacag ccttacctat aaatttatca acagttaatt tgtcgagaga
      601 gcaactgttc tcaaaaatat gagaaggtgc atctatatga gtccctgtat gagaacaaaa
      661 tgaaagagat ttctctgcat aattatccct ttcaatagtg ctctcctgag ttatgacagg
      721 tgtagatgtg cctggaaaaa cagacatggt agattctata gtgtgtgtca gatctataac
      781 tcttaatgaa ttatttcgca tagtacaaaa atatctgatt atatttcaaa tatactgcat
      841 ttaataaaaa atgacattct taaagggcac ggcttttgaa caacgcttaa aaatatttat
      901 acttgaagtt taattacaac ttcagcaaat catgaatcca atcattcata tcaatccaaa
      961 atacttatat ttaaaagatt tcatattaaa tatacctgca caattttatg aaatgggtat
     1021 tcctattcat acaggcagaa atgaacttcg tcttgtagaa acagaaaatg ttaaaattgt
     1081 tataaaacat ttcaaaagaa taacagaaat aaacagattt atttacgcaa attttagaaa
     1141 gtcaaaaggc aggagatctt ttgaacattc aatgcaactg attgaaaagg gattcgcttc
     1201 tcctgagcca gttgcttata ttaatttaat aaaaggcggg atacttactg atgactatta
     1261 tatctgtcta tattgtgatt tcagaccatt caaggaattg ctttccaaac ctttggccga
     1321 agcacagaaa gggctcgaag aatatgcaaa atacacttac aaattacata aatgcggcat
     1381 attccacaaa gattacagca cgagcaatat tctttttaat gataatggcg gcaattatga
     1441 attttcgcta atcgatgaca accgtttatt attccgatca tacagcaaga caagaagtat
     1501 ggtaaccatg gtaaaaaggc aggatatctc tgtcgaacaa atgggtataa tagccggctc
     1561 ctatgcgaga gctgcaggtg aaaatgaata tagtatttta aacaacatga cccgacaagt
     1621 actgataatt tcaaaagtat tttctgtaag aagacgaata aaaaagagtt tgaaggcaat
     1681 aaaagttaaa atgtagttga actatataat attgatacat ttgcaattca taatcagact
     1741 taaataagta ccataaaatg acaaactatt taacaaacga aaaaaaaatc gctaacctta
     1801 agggacttat tggtaaaacc cctcttctga aaatacacta ttcctataag ggagaagaac
     1861 gtaatttata tgctaaagct gaatttctca actttaccgg cagtataaag gacagaatgg
     1921 catactacat aatgtgggat gctgtcctca cagggaaact gaaacccgga atggtagttg
     1981 ctgaagcaac cagcggaaat accggtatct caatagttgc aataagccgt tttctaggtc
     2041 ataatgtttc aatctacatg cctgactgga tgagtagcga aagaatagag cttattaaaa
     2101 gttttggtgc caatatctgc ctcgtttcaa aagaagaagg aggatttatt ggtagtatag
     2161 aaatgacaaa caaattgaaa agggatgatc cagacggagt ttttctgccg ttgcaatttg
     2221 ataacgagat aaattgcaaa gctcactact tttcaaccgg tcctgaaata tacaatcaat
     2281 tgaattctca gggtataata ccaaatgcat ttattgccgg agttggtaca ggcggaacaa
     2341 taatgggaac aggtctattt ctcagagaag catatcccga catcaaggtt catcctctgg
     2401 aacctgccag ttcaccaact ctttctacag gacattgcgt aggaaaacat agaatacaag
     2461 gcatttctga cgaatttata ccatcgattt tggacctcga caaacttgac gaagtgattc
     2521 aggtagacga tggtgattcg attattatgg cgcagatgct tgcgaaatca ttcggtcttg
     2581 gagttggaat ttcttcaggt gcaaatttct tgggagctgt cgaacttcag aatcgtcaca
     2641 atagtgatta tgacgtagtt acggtatttg cagatgataa caaaaaatat ctttctactg
     2701 attactcaaa ggaagagcct gttaaaccaa actttctgag caaggatatt cgtttgcttg
     2761 agatggttgc aattccttat tattaataga taattaccaa tgaaaaagtt atttcaaaat
     2821 agtgtaatag tcagtattct gattgtatta ttttccaccc taaacatttc cacatatgct
     2881 gccgttggtg gcaaacagct tgacagcacc gtttttgaaa cttggagaac atgcgaatca
     2941 ataaaaatcg ctgatgatgc ttcatggagc attgccgaat ataaaaacaa ggaggatagc
     3001 agtattctcg ttattaaaat tgacaagatt aatttcagga aagagataaa aaaaggcaag
     3061 aatcctcaga tgtttaacaa tatggaatca gctgttttct ttgttaaaga tactcttttt
     3121 acaattaatg gacatgaagg cacaattcaa aaaattggaa taacaaagag tgcctcggtc
     3181 aatgacaaat cccctttttt cacatataag aggggaaaaa ctttgttcat tagcaatgtt
     3241 aaaaacggct caacagattc aattaagaat gtcggaacat cgatattcat aaatccagag
     3301 acattgactg ccacagtcaa tgaaggcaaa agcaatatta tactgataat cagacttgga
     3361 aataaaaagg gtattgacac aacacaaatc tataagactg ataaatttat tactgatgta
     3421 tcctctaata aaaatggcga caaacttata ttttttacat gcatcgattc tctgttgaaa
     3481 tcacctgaag tgacaatgat aaagatcaaa gagagaagtg ccaataaaat taacctcaat
     3541 gaaagtctgt tgcctgaagg tatgcaattc aatatcaaga aaggcatagt cttttctcaa
     3601 gatgacagtt atctaaaatt cgaaatcgaa cctgtaaaag cgaaccacga caaatcgaat
     3661 gagaaagtca gcaagcctcc atttgatctt gaactctgga aatggaatga tactttgatc
     3721 cctttacagc aaaagagagg gcctaaagct tttgaaaaaa gtatgaaatg tgcattttat
     3781 cctgaaacaa acaaatttgt acaactttcc acaggcaaag gtgtctatct ccagatagat
     3841 gataattctc catacggcat tgaatttaac gaacgtcctt acctttatcg ttccaactgg
     3901 gaagatccta ccccgagaga tataatatgc attgatgtta aaacgggaga gagaaagata
     3961 ctgctaaagg aatttaaagg ctctatcgcc gtatcaccca gaacacctca tctatttatt
     4021 tttgaaccgg aaaatgggga atggtcaata atagatacta aaagtggtgc aaaaactctt
     4081 atttcagacc tgattgacta tccactgcaa gatgaagagt ttgaccgccc tcagccagca
     4141 ggcagttacg gtcaggcagg agtttcaaga gacaatcaat attttcttgt ttacgacaag
     4201 tatgatgttt gggcactacc aacttctggt attggagagc ctctctgcat aactaacgga
     4261 tatggaagaa aacataatat tcgcttcaga atactcaaaa ttgaaagtga cagtgatcaa
     4321 aataaaaatc gaggggtgaa cttaaaagag acaatctttc tcgaatcact caatctaaac
     4381 aacatgtatt ctggatatta caaggtggct cctggaaaag atcctgtcaa gcttatagaa
     4441 gatgcttgta aattttcttt ctacaagaaa ttgggtaaag acaactatat cttcaagaaa
     4501 gaaagctttg atgaagcacc tgacttctgg cttagcagta aagattttca acctgtaaga
     4561 agacttactg accttaatga acagatctgc ggatatttta ttggaaaatc aaaagtaata
     4621 acatggagtg atggctcagg aaaagaacaa aaaggggttc tttatactcc tgcagattat
     4681 gattcatcaa aacaatatcc tgtaatagtc tatttctatg aaacaatgac ggaaaacact
     4741 ttcctgtttt atcatcctct tcctacagaa tccacaatca atccaattct attcgttagc
     4801 cgagggtatg tggtcttcat gcccgatatt cattatgaga taggatggcc tggaaaaagc
     4861 tgcgtaaata cagtggtaag tggcacagaa tacctaataa aagagggcat tgcagatcca
     4921 gaaagaatag gagtccaggg acatagctgg ggaggctatc aagtagcatt tctcataact
     4981 cagtcagata tattcaagtg tgcatgttct gaggcagcag ttacaaacat gacatctgca
     5041 tatactggaa taagatatgg ttcaggttca cccagaatgt tcatgtatga atgttctcaa
     5101 agtagaattg gaggtaatct ttgggaaaaa cagaataact atatattgaa ttctcctact
     5161 ttctttctga acagagtaac cactcctttg ctttcaaggc actgtgatgc agatgaagct
     5221 gtaccatact ctcaaggttt ggaattattc ctggggctca gaaggctcgg caaagaggtt
     5281 tggatgttca attacgaagg cgtaggacac aatatcaagc catgggaagt caaaaaagac
     5341 tggactaaaa gaatggatga atattttgac tattacttga tggacaaaga aaaaccatcc
     5401 tggatgtaaa attaatttcc aatatcgaaa ttatactttc taatcagctc ctttaacgac
     5461 ttttccggta atagagtatt aaagtttccc cagaaggctt catcataact atatgacact
     5521 tctgagagaa aatttttgtt tgaaactatt tcttcatttg caaatcgctt tactcctgat
     5581 gtattagttt cgcagtttgc catttcaaac cagacatgtt catttgaact aaatagactg
     5641 cctctctttt tcattttaag ggtgaggtca gctcttacat gattaagata gtatttgcca
     5701 tcgaccggcg catacgaaac ttcgtaaatg gcactttgag gtatagcttt aatatgacga
     5761 ctctgcttca aaacaagtat atccgctgca tcttcaacat agagaggatt gatttggaaa
     5821 gttgccttga gaagagcata gttttctgca tcaatgtaaa gtttacccat aaaaagagga
     5881 tcttttattt cttctttttg ttcaaagaaa aaaacgagca ctcttctctc atcaagcact
     5941 gttatgtcag tatgtgagta attatagtaa ttgtaatact gaggatccag aaaatcaggc
     6001 agatttttta taatatcgag catttgacat gaataaatgc tcgatttaag tcgcacaata
     6061 agtgaatcat tataattggg agttccaaga cgcctcattt ttaatatctt gacctgttca
     6121 ctcgccaatg tgctatgaat acctgtttta tatattttta aaactgcttc ggtaagatta
     6181 aatccgtgcc tattttctat accctctcta aaaaatgagg ttaaataggc tgtctcgcta
     6241 ttataattat cctgtctgcg ttttaacatc tctctcaata ccttctttgg atctaccatt
     6301 cttaccacta tctcctgaag aggtatgatc ttctgatcaa gatatatgtc tatattctta
     6361 ccggcaatta catcagcact tatcattctg ttgagataac caatatgtga gattctaata
     6421 accgaggatt gaagggaatc aggaagttta agcataaagt tgccatcttg gttggaaaca
     6481 gttgtttttt tcccacaagc tacatcgatt gtaccataaa ctacaggctc tccagttatt
     6541 ctgtcaagca gcctcccctt aacaataaaa acagaatcgg ctgaagattt taatttcaat
     6601 tcagatgtaa tttctgtatt accctttgaa ataaaaagga gcaaatattt atcctgcaac
     6661 ttaacatcaa cattattacc agttatttct cttattaaat ccttgagttt ataatcacct
     6721 ggattgattc ttactttccg gtcattattt atcaactcgc tgtcatatac aaagtagtat
     6781 cctgtaatat ctgacaatct gttgatcatc tgatatactg tccctttttt aagatcaaac
     6841 ctgatcatct gttctcctgg atcataatta accagctcct gagaccaaac caaaggagag
     6901 gtctctacaa taatacctat tgtcaacaaa gcacagataa acaatctatt catagattac
     6961 aataacatct cctcttatct cataattaag atcaagtgca aaacaaatca tttgagcaat
     7021 tgattcagtg gatctgtcaa taaatgtagc agtcagactg cgctcaggca atttttcatc
     7081 atctgcaaga gtcaaagtta agtccccgct ttttttcatt ttatttacaa cctttactac
     7141 atctctcaac ttttcatctt tgaaatgtat tctttcagaa taaacagcaa atttagataa
     7201 gtccaatgtg ggaactatcc gtaaagagtg tctctcaaca aaggctgtct cacctgcaac
     7261 aaccttctgg ttagtgccat tttctttaag tgtaattttg acaataccgc tcctgacaga
     7321 gagtgatgga catgaatgat tctccgtctc tacactgaaa gaggttccaa gtactttgat
     7381 ttcaaccata ccggcatcta caataaaagg tgtttctgta tttctgacaa cttcgaaaaa
     7441 agcattccct ctcagataga tcttcctgac ttttccgaac ttggcaggaa aggtaatttc
     7501 agaaccattt tcaaggaaca ctattgaacc atcttcaaga gttttaacca gagtagaatt
     7561 gtcattgttg ctgtatgaaa gcatttttcc aacattatta taccggtcta tgcccaaata
     7621 tacggatact aaaaccacaa tcatcccaat taaagcccaa cgtagtgtta tgttgtgcaa
     7681 catcttccct ctccttcctt tagaattttc agtatataac aaaccctcgt tattaagacg
     7741 atcatacaat ttgctccacg caaggtttgt catttcactg ttcttttttg aattcatgat
     7801 gttcaatcat tatatttttt tacctctctt cgtaatgttt ttaaagcctt accaatctct
     7861 gcctcaatag ttttaataga aagtgaaagt ttttctgcaa tttcggagta gctcatttta
     7921 tcaaaacggt gcattctgaa aatttgcgct ctacgtatcg gcatcatctc catactcttt
     7981 aaaataacat tttcaagatc tttcaactcc atattactct ctccatcttc accggtgttc
     8041 atttcaactt ccgaagaact cttgaactgc atattgaaac atttactgtg aagatattga
     8101 attgacctgt ttttcaccga tttatacaaa taacctttta gtgaactcat tatttgaagt
     8161 ttatccctct ctttccagat ataataaaac aactcctgaa ttatctcttc tgatatctct
     8221 ttactgccgg taatacttat cgaatagtag agaagggaca tatagcttga tttgaagagc
     8281 tgttcaaaag cagctatatc cccctcctta attctcttaa ccagcaaaag atcctggagc
     8341 atattgaaaa ataaataaat atttattcaa atataggaaa aggatgtggt tattttttat
     8401 taatcgtttt tctaagttta ttgaccgcat tttcaagaga ttgctcaggt tcaataatgt
     8461 tataatcttc ccagaaattc tcatcataga agtcatctac cttgtcagag agagagtagt
     8521 tatcgttaaa ggcatatttc tttgtaaatt taagtggtat atcattagac accttagtta
     8581 taactgtttc tgcaactatt gcataatttg tagagaaaag cttcttcttc cagtcacact
     8641 taaattgtat ttcgctttta acatagttaa gatagctttt gtctcccagt ctcttgtatg
     8701 ttaccagata cgaaacctcc tctggcttaa atctcaggct aaaaggtttt ttcttgagaa
     8761 tcatctgtgt taccttgtta cggtcatcca tgctcaggct gaattcaatc cttgaaaatg
     8821 taagcgattc tctatcaatg taatatttgc cgtaataaag tggatatggc agcacaacct
     8881 gcggggcaaa acttacaaca taattttgtc tatcatcaat cataaccggc atttccattt
     8941 tataagtata atctcccaga gaagcaggat ttaacattaa gtcgggattt ttgacaacat
     9001 ctaaatagat tgatagatta ggacctccaa gaagttttac tactagtgta tcatttgcct
     9061 tgggagatac aattcttctt cctttatata cctgtactcg gtctccttca taattatcag
     9121 tatattttgt cttaaatatc tcaactatgg cctcggccac atttacataa gtgcttcttt
     9181 tcctgacagt ttctctgtaa aatccggaaa gaaggttttc ctgattgctg aaattatctc
     9241 ctattctgct gattgcctca cgtataatca tttcagggtc cattccaaga atagttatct
     9301 ccgtcagaac attatccctt ggcgaaagat aaacaacgag gttatcaata tttacaccct
     9361 ttacggctat cacctctgat gaatatccta tacgagatac ttcaatagtt ttggcattaa
     9421 gttcgttttt tatcttaatc gaaaattcgc catccttgtt ggaaactgtc ccaatattag
     9481 taccgctcac aaatatgttg gcatactcga gagttttatt agtcgtcttg tctttaacaa
     9541 cacccttcac tgtgtaaaat cccttttcag cctgagacaa tgcaggtgta actgtcaata
     9601 ttggcagtaa gagggaaaga agagtagcta aaattttaat tctctttttc atatcaattt
     9661 gcattaatgt atctattcgg tttacataaa taagactgtc aaaaaaggta aaaccctata
     9721 attttttcaa tttttctttg attttgtaat catattataa tttttgtaaa tttactcggg
     9781 tattttaaac aaacacacaa ttaaatggac actaatgctg aaattcttgt cgataaaaga
     9841 actatcgacg agattatttc cagaaatgca ggcaaaccag ggatgctgct cacctccatg
     9901 gaagagattc aggaggcaaa tgagtataaa tttcttgacc aacagagtat ggaacacctc
     9961 tcttacagaa ccaaagtgcc gctttcacaa atttacagcg tagcaacttt ttactctttt
    10021 ttcaacctta aacctcaggg taagcatact ataattgtat gcagaggcac agcctgccac
    10081 acaaaaggtt ccaaggtgct gcttgaagac cttaacattc ttccgggatg taaggatgct
    10141 tttgataacg gtgagaccag tttcactact gaagatagac aaataacagt caaaaccgtt
    10201 gcctgtttcg gacagtgtgc ccttgcacct gtagttgcag tagacggcat gatctacagt
    10261 aatgttactt ccgaaatgat tctgaaatta ttacaaaatc ttagagagga caaaaaagat
    10321 gagaatatca aattatgctg acttggaaga gatacgctta aaagctgcca caaaaattct
    10381 ccccgacaag ccttatattg ctgtaggtat ggggacttgc ggcataggca gaggagctga
    10441 agtcctctac gacagttttc agcgtataat taacgaacga aaactagaca ttatcctcaa
    10501 aaaaacagga tgcttcggtt tttgcgccga agaaccatta gtcaacattt acatacctgg
    10561 aaaaccactc gtcatattgc acagactggc cgaggtagat gtaattccag tcgtttctgc
    10621 cattcagaaa ggcttgatgc ctttcagaaa cgtgctttgc cgcatagaag actgggattt
    10681 tattacaggg aaatttgaat ttggcaaggg gttgactgaa catcctcttt ggaatgaaat
    10741 tccatttttc aaatggcaaa agaaaatcgt gcttcgcaac tgtggactga tagtgcccga
    10801 tgatattgaa gagtatattg gagttggtgg atactttcct ttatatagcg ttcttacggg
    10861 taaatctcca caggaagtca tagatgaggt taaactctcc aaactgcgtg gacgtggtgg
    10921 agcaggattc cctacaggtc aaaaatggga actaatggcc aaagtcaaag ctgatcaaaa
    10981 atatgtcata tgcaatgccg atgagggaga ccccggagca ttcatgaata gaaacgaaat
    11041 agagagtgac ccgtttatgt tgttggaagg catgactata ggcgcttatg ccatggatgc
    11101 atcgaaaggc gttgtataca taagggcaga gtacccactt gcagtaagac gcctcaaggg
    11161 agctattgag caggcaaaat ctctgggatt gcttggtgaa aacatattgg gaagtggatt
    11221 taattttgat attgatattg ttgagggagc tggagctttc gtgtgcggtg aagagactgc
    11281 tcttattaaa agtattgaag gcaaagcagg aagaccagtc ccaagacctc cttatccggc
    11341 tgttaaaggt ctgtttgact accctacaaa tatcaataat gttgaaacat ggtgcaatat
    11401 acctgtcgtc atgtccaaag gagcctcctg gtttacagaa accgggactg acaaaagcgc
    11461 gggaactaaa gtattctctc tggtagggaa agtcaaaaat accggtttag tagaattgcc
    11521 tctcggttct actcttgaaa caattgtgtt cagaattgga gagggaagtg gttcacaaaa
    11581 gagagtgaag gctgttcaaa caggcggccc atccggagga tgcatacctg tggactacat
    11641 gaagactcct gttgactatg aatctctaaa tgctctagga gccattatgg gatcaggagg
    11701 aatggtggtt atggaccagg ataattgtat ggttgatgtt gcaagatact tcactgagtt
    11761 ttcaaattcc gaatcctgtg gaaaatgtac accttgtaga gaagggcttg cacagacact
    11821 tgaaattatt aaaaaattta caagtggcaa agcgcaggag gaggatataa aaacattgga
    11881 ggagctcgga caagtgatta aagctacagc tctttgtggc cttggacaaa cagctcccaa
    11941 ccctgtactt actactctga gatacttcag ggatgagtac gaccatcata tgattgaaaa
    12001 gaaatgcgag cctggtattt gccaggatct atttactgct ctttgtgaaa acagctgtcc
    12061 gatgcatatg aatatccccc gttatctcca gctgcttaaa gaaaataagc ttgaagatgc
    12121 atttgaatca tcattaagag acaaccctct gccttcaaca attgggcgta tctgtcactt
    12181 ccactgtcaa atgaaatgcc gcagagaggg aatagatgca cctgtatcac agggcgaaat
    12241 tcacagatat atatctgata ctgtctatgc caaagaaagt tataggaaaa tttatgacag
    12301 actgattagc gaaaagatgc caccaactca caagaaaatt gcaattgtgg gaggaggacc
    12361 tgcaggattg actgcagcat actatcttgt tagactcgga catgacgtaa caatttatga
    12421 ttctgaatct gaagcaggag gtattctcag atggggtatt ccaaaataca ggcttcctct
    12481 cgatattctt caaagagaga ttgatttcat aaaagagctt ggtgttaaat ttgtcatgaa
    12541 tacatgtgtg ggaaaggata tctcacttga acagttagaa cacgacaatc atgtggtcat
    12601 tcttgctgtt ggagctcaaa aggacactga attgcagata cctggttgca atcttaatgg
    12661 ggttatctct ggtatggatt ttcttaaaga aatagctcat gagaaaaaac ccgaaatagg
    12721 gcatcatgta gtaattgtcg gtgccggcaa tgtagccata gacgcagctc gtacagcctt
    12781 gagattaggt tccgaggtta cagttgtcta tcggagattg aaagctgata tgcctgccaa
    12841 taaggatgag atcaaggaag ctgagaacga gggcgttaat ttcttgttcc tctgcaatcc
    12901 tgaagagata cttgcagacg caaatgacaa agttagaggt gtcagactta tgaaaatggc
    12961 ttgtggagag gtagatcttt ctaacagacg caaacctgtc cctacaggag aattcttcac
    13021 gctcgactgc gacaatgtta ttatggccat tggagaagaa gttgatgctg aatttgcaaa
    13081 agaatatggt ataaacctta aaagcaatgg aaatattctg gttgaccatt tcactcatga
    13141 aaccaatcat cctaaattct atgcaatagg cgatgcaatc acaggacctg ccactgcggc
    13201 tgaggccatg ggaatcgcca agaaagctgc agaagtgatt gatcttaagt tgatggataa
    13261 gaaccgcttc cacaaactct tcagagaatt tgaatacagt aatgaggttc cgctcaagcc
    13321 cgaaggagga aaccgtcatt attcagttaa gctgccttta gaagagcgta ttggtaattt
    13381 taaagaaata agtttgggtt atacacctgc ccaggcatat gctgaggcag caagatgtct
    13441 tagatgtgat gttcgcacta ccgtcaaaag agaggaggaa taattatgga aaacaataaa
    13501 gttaatatta cagtggacgg cagagaatat caggttccgg ccggagccac tattcttgaa
    13561 gcatgcagag aaattggcat tcagattccg tctctctgtt atatgaagga cgtgtctcac
    13621 aacgcctcat gctccatatg tgttgtcgag gttaaaggag ctaaaactct tgtaagatct
    13681 tgcatcagta cagttgccga aggtatggat ataaaaacac actctactct tgtagaccag
    13741 gctcggaaaa taaatcttga gcttattcta gccaaccatc ctaaagattg tcttatatgt
    13801 gagagaaatg gaaactgtga gctacaggat ttgacgtcaa taatagggat aaaggagact
    13861 ccttttttaa aaaccaaaac ggccgctaag gttgacgata cctcactttc actacttaga
    13921 acgcctgaaa agtgtattct ttgcggaaga tgtgtggcag tatgtgctga agttcagaca
    13981 gttaatgcga taaattttac aggtagagga ttaaaaacca aagtctctac atatctcgac
    14041 aagggacttg gcaatgttgc ctgcacaaat tgcggacaat gtgctttggt ctgcccaact
    14101 gcagcaatca ccgaaaggga tgagtcaaga aatgttttca atgaaatata caacaaggat
    14161 aaatttgtcg ttgttcagac tgcacctgca atacgtgttg gcatcggaga agcaatgggc
    14221 atggaatatg gcgcccttgt taccggtaaa atggtggcag ctttaagaag agtgggattt
    14281 gacaaagttt tcgatactca gtttgctgct gacctcacaa taatggagga aggatatgaa
    14341 cttattgaaa gaatcaaaaa taatggagcc ctgccaatgg tgacttcctg ttctccagga
    14401 tggataaaat ttattgagca tttcttccct aaatctctag accacctctc aacctgcaag
    14461 tctcctcaac agatgttcgg agctgttgcc aaaacatatt atgcagaaaa gttaaacata
    14521 gatccccgca aaattgtggt tgtttccatt atgccttgta ccgcaaagaa atttgaatgt
    14581 aaaaggcctg agatgaaatc tgcctttaaa tactggaaag ataagataaa tctcaacgaa
    14641 aacgaggagt tctttgacgt agattatgta ttgacaaccc gagagcttgc aagaatgttc
    14701 aaacagtccg ggattgactt caaaaatctc ccagaagaag aatttgacga acctcttggc
    14761 atatcatcag gagctggtgt aatattcgga gctacaggtg gagtaatgga agctgcagta
    14821 cgtacagctt acgaagtcct taccagtaaa ccacttccaa atattgatct aaaagctgtg
    14881 agaggattcg aaggcatcaa ggaagcagaa ctcgacatca atggcatgaa gcttaaagtt
    14941 gccgttgccc acactcttaa gaatgctcgc actttgctcg atcagattga agaaggaaag
    15001 tcgccatacg cattcattga ggttatgacc tgtcctggag ggtgtctcgg aggtggcgga
    15061 caatctattc ctactacttg ggagatccga caaaaaagag ccgactctat ctacagggaa
    15121 gattcattga aaccaatcag aaaatctcat gaaaatcctg ccattatagc aatatatgaa
    15181 gagttccttg aaaagcctct cggccatcat tcacacgaat tactgcatac aagctacgtt
    15241 gagcgaggca ttttctaata gataaatatt ttaagagggt tcatgatttt ttctcatgtt
    15301 ccctctttta ttttagcagt ataacccaaa tggtatagtt tatgcgaggg ttcttattcg
    15361 tcctaatatt ataaaatcat gaaacatcca tgactaaaaa ataaacgcca aaggggatga
    15421 aaatatttta ggataattta gatttgttct tacaaatcta aattaccagg aaatggaaga
    15481 agtatccttt cgtcaagttg tatcacgagg ctgtggtata ga
//
LOCUS       DAOTQL010000025        15041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3815_length_15041_cov_5.163286, whole genome shotgun sequence.
ACCESSION   DAOTQL010000025 DAOTQL010000000
VERSION     DAOTQL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_15041_cov_5.163286"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="P5293_02805"
     CDS             complement(<1..1244)
                     /locus_tag="P5293_02805"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR48865.1"
                     /translation="MSNLLDSDIKYLPGIGPKRAELLASELNIRTFREMLYFFPFRYL
                     DRTKIYSISEIDPDMSHIQLRGRIVRINLIGEGGKKKRLVATLADATGVVDLVFFNGI
                     KWIKNKIKINSEYIVFGKPTIFNQSINFIHPEIDPPNEENSLYSGNMIGIYSSTERLK
                     SSGITNKQIAKYQSLLLKMTSDAIKETLPSYLIENKKLLPLRNALKMIHFPDNQVSLN
                     LAVFRIKFEELLYLQLSLLRQKNLRERFTTGFMMPRVGKYFNACFQNIPFELTNAQKR
                     VIKEIRSDLVSGKQMNRLLQGDVGSGKTMVAVLCALLAADNGFQSCIMAPTEVLANQH
                     YKSALKIVEGSGIEIALLTGSTKSYERNKILEGLSQGKIHILFGTHALIEDRVKFNNL
                     GFAVIDEQHRFGVEQRARLWTKS"
     gene            1311..2372
                     /gene="pdxA"
                     /locus_tag="P5293_02810"
     CDS             1311..2372
                     /gene="pdxA"
                     /locus_tag="P5293_02810"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRR48866.1"
                     /translation="MINNKIVVGISQGDINSISSEIIIKALSDSRLLDTCIPVIYGSS
                     RVIGIYKKHSPDTENFSVNVINSSKDAHAKRINIINCIPDNYPFEPGRPSEEGAKAAM
                     IALEKSVNDLKNKNIDVLVTGPFNKFEAKRSGFNFTGHTEYLANVFAVSNPLMFMISD
                     RIKVGLVTNHIPIAQVSSSINSGLIVEKLRIMSASLKRDFAISSPKIAVLGLNPHCGD
                     QGLIGNEESDIIKPAIHQAFDEGILSFGPYSPDGFFGSEMLCKFDAVLAMYHDQGLIP
                     FKTIAFENGVNFTAGLPVVRTSPDHGTAYDIAGKNKANPASMLSAIYWACDIFKNRKY
                     YDELRSNVLSSDQYISEKQ"
     gene            2372..2854
                     /gene="rnhA"
                     /locus_tag="P5293_02815"
     CDS             2372..2854
                     /gene="rnhA"
                     /locus_tag="P5293_02815"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRR48867.1"
                     /translation="MIEIFTDGASRGNPGPGGYGVILKSGKHYKELSGGFAYTTNNRM
                     ELMAVIKGLEAIKVQKADICVYTDSSYICNAINQGWLFKWEKKNFEKKMNADLWKQFL
                     PLYRKHNVKFIWIKGHAGHPENERCDRLAVEASMNPDLPVDTGYTCLPECNPDSSTKD
                     "
     gene            complement(2775..3899)
                     /locus_tag="P5293_02820"
     CDS             complement(2775..3899)
                     /locus_tag="P5293_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HRR48868.1"
                     /translation="MELDNLYTKALNLEPLNCSEGMELFQRAPVNELFSLAKRLRYIH
                     NPESKVSWQIDRNVNYTNVCISGCIFCNFHCNISQKEKSFTITESQLEEKITELRKLG
                     GDQLLLQGGLHPEYDISYYEELFRKIKAIDPLIKLNALGPPEIAHIAKLSNLSIKETL
                     VRLSLAGLDTLPGAGAEILSNRVRKIISPAKPGVESWIEVMEEAHKLHIGTTATMVYG
                     HIETLEERIEHLILLRDIQNRKPAEAPGFRAFISWPMQTADTKLAEKFKLRQLSLIEH
                     LKMVAISRIILNNVDHIQVSWLTIGKQAGMIALHCGADDMGSIMIEENVVSSAGANNS
                     MNAEEMQKAIREAGFNPWLRNQDYTPVNKYNQCLQEDLDS"
     gene            3975..4985
                     /locus_tag="P5293_02825"
     CDS             3975..4985
                     /locus_tag="P5293_02825"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRR48869.1"
                     /translation="MEKYIERKNSNLAVIGAGSWATALAKLLLNNCGEISWYIRNKRV
                     LESIKTTGHNPLYLEDVHFDTSKLKISNDINKIVDGADVLLFCIPSAYFLPEVAKIQN
                     LKDKFIISAIKGIIPGDNITIAEYFHRVHKIPFSRIGVISGPCHAEEIALERLSYLTL
                     SSKYRETALRLCNLFSCNYIKTIPGTDIFGVEYAAVLKNIYAVAAGVATTLGYGDNFM
                     AVLVTNSFHEMRRFLHVAHPDSERSTTKSAYLGDLLVTCYSKFSRNRTFGTMIGKGYS
                     VRSALLETKMVAEGYYGTKCIWELNQKFNVDMPVADAMYRILYGRENAASVMKELTSK
                     LE"
     gene            5011..6351
                     /locus_tag="P5293_02830"
     CDS             5011..6351
                     /locus_tag="P5293_02830"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRR48870.1"
                     /translation="MEKAVKINLEAINGFLTREEIIDAKKRALASFDTLINRSGRGND
                     YLGWLDLPTNTDEAVLQRIDQIVALWKNKIEVVVVIGIGGSYLGAKAAIDALSHSFGA
                     YLGKRSFPVILFAGQNLSEDYMAELFDIIDKKSVACVVISKSGTTTEPAVAFRMIRKS
                     MEERYGKVEAANRIVAITDKSRGALKELADSEGYETFIIPDDVGGRYSVLTPVGLLPI
                     ALAGFDIRSLLRGACLMQKICHLRDDNNPAIEYAAIRNALYNKGWKIEILANYNPKLQ
                     YFAEWWKQLFGESEGKDGKGIYPASVNYTTDLHSMGQYIQSGERILFESVLSVENPSR
                     CAVIPEDARNMDGLNFIAAKRIDECNKMAEAGTRIAHVDGGVPNIRIIIGNISEYSLG
                     ALFYFFEFACGISAYMMGINPFDQPGVEDYKNNMFALLGKPGYEEIAEKLRRRV"
     gene            6351..9092
                     /locus_tag="P5293_02835"
     CDS             6351..9092
                     /locus_tag="P5293_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRR48871.1"
                     /translation="MDFLNSIATAFYEREKGLISEYCFVFPNRRASLFFQRALASIIR
                     EPVFSPVIVTINDLFEQLSSLKQVDRIEALTELWTLYNSISTKKESFDEFIYWGDIIL
                     SDFDDVDKYLVDAGKLFANIQDLKEIESDYSFLTQRQLDAIHQFWYNFFPAGSSENKL
                     KFKEIWEILAPLYYRFTEKLSMRSIGYEGMIYRKVASEILSERYSENSSFKKLFLNRK
                     IVFIGFNALNPCEKTLMDLLKRRGMADFYWDYAGEMISNSDNLSSFFIKDNIKRYPSE
                     IEIAPYSCGKCSFEVIGVPSTIGQAKRVMNILKEVGGDINTAVVLPDESLLMPVLYSI
                     PEEVKDVNVTMGYPLKEGGIVSLVGNILELQKGVYYYRRVLQVLQHNYIQVIAGNEAN
                     SLIERIYQGNVIYVGAEEFNVHPLFRLIFKETNDISSYLLDILEFLNRSVQLTKIEKE
                     FIFSIYTSVMRLKDLLPEVSADTYAKILSQIIATSTIPFKGEPLSGLQIMGVLETRCL
                     DFENVIICSMNEGIFPKRSFNNSFIPVTLRRGFSLPDFDYSDRVSAYLFYRLISRAKK
                     VYMLYDTRSEGLKSGEVSRYVYQLKYHFGLQLQESIAVQRVELPSIREVVINKDEGTI
                     LQLKKLYFPLGESALSASAINTYITCSLKFYYRYIKGFKENENVAEGVEADTFGSIFH
                     NTIADLYKIFKGRRVTSEMLKELSRDSVKIEKAIDDSFLKFMNLKEVRGRNLLTKKLI
                     LKYVKQVFIFDTAVAPFEYIDSEMVVNREVELKDGFSLHLKGVMDRLDRIEEGKRIVD
                     YKTGYGKIPKNIDLAKLFSENHDKGDEVVFQLLFYMLIINDKDVSFFEPYLLRNLFKK
                     DRYLQNVNEDMLLSFSYQLGDKLSELFDITVPFRQTSREQNCEYCPYSKICHRYGKS"
     gene            9079..12054
                     /locus_tag="P5293_02840"
     CDS             9079..12054
                     /locus_tag="P5293_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRR48872.1"
                     /translation="MGNLKILKASAGSGKTYNLTKEYLNLIEKEREIFSHILAVTFTN
                     KATEEMKHRVIKELHKKSLSYPVAKERLIEILHNYSNFNISTIDRFFQQIMRSFAREI
                     GKSGSYNVELDDNLILVEAVEMMLQDLDIEENEQLLDWLVKFSIDAIEDGKSWDIKKG
                     IIPFAQQMFKEAFRAIKSDFKDSVNNKITLQEFISNLDGVIKKYEDEILNITEEVFRV
                     MERYSLSCESFKGGTRSKFKFYDKTASGDYYSVSPNSLMAFTEDFDNWFAKSVIKSNP
                     VLFDSISNAYNESLRELVEQTVSLQNDWIEAHSAILVRSRIYILGILIDINKNIQRYS
                     KEHNIMLLSDTTEILNNIIDGSDSPFIYEKIGSRIDHFMLDEFQDTSLMQWNNFKPLI
                     KNSLASGNSNLVVGDVKQSIYRWRGSDWSLLNAEIYNDFGRDDIEESVLEENWRSYEN
                     IVNFNNKFFKYAANQCDILIGDSIIAQKIYSDCEQKVCKQNIGNSGHVKIKFIQKEEK
                     KWKENVLNELPLIVLKFRENGYQFKDIACLVRTNKDGVSVVNKLVEKGLPVISNESLL
                     ITSSRGVRKVISILRGLVNKNDLFSKAIAELEGLKPDYDDDILRLPIYEICERVVESL
                     EDEIQETDHPYIQGFMDAVLDFLANNSPDLNLFLEWFDKQWYKLSISSPEEQNAIRVM
                     TIHKSKGLGIKAVIVPFFDLELDHTGNPIIWCKPDRAPFNSIPFLPVSYKKELEDTIF
                     SADYKMEKQRSYIDNLNLAYVAFTRAREELVVYAPLPSEKGTNASLANILYNLLKEEL
                     NPDLECNLGEWCHESTPENSRTTTYYDNYNSVPPGDRLHLSLRAGEFFDKQSKRNYGL
                     IMHDILSGVYTEKDLLPCVKKAVAAGEISADEEIKILDYLTRIMESVEERHWFDSSYT
                     IYNELEILSPGGRISRPDRVLIGDDKAIVIDFKFGNVKDSSYIKQVANYKKLIEKITG
                     KSSEGYIWYLEDNDIVEVK"
     gene            complement(12047..12976)
                     /locus_tag="P5293_02845"
     CDS             complement(12047..12976)
                     /locus_tag="P5293_02845"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HRR48873.1"
                     /translation="MTEFKKRLIILSGILLVLSSCKNVNNNEQNALKGELKMIEVPSI
                     ITDNYERNLYIVKHFWDGVNFSDTSYLVNQQGLNTHFSAYLEYLTTVPHDMALASVKA
                     LANRAILGHPKMMMALQEMFEKGFYHPNSIFKNEELYIGVLEEYIKSDKLDSAIKEKL
                     TKQLELAMRNRVGTKAIDFSFIYPDGQRGSLYNIPAEYLILIFFDPDCPSCKSTMENM
                     EKSQVIASFGGKVKVLTMYAGVDFENWKSFAPLLNKKWLNGCDKDLVIMNNSLYDLRP
                     TPSLYLLDKSKTVILKDAPFEAIESYLKNNTTI"
     gene            13061..14473
                     /gene="gnd"
                     /locus_tag="P5293_02850"
     CDS             13061..14473
                     /gene="gnd"
                     /locus_tag="P5293_02850"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HRR48874.1"
                     /translation="MRKTDIGLIGLAVMGENLVLNLESKGYSVSVYNRTKAVTDGFIR
                     NRAIGKSIFSYYTLFEFINSISLPRKIIIMVKAGEAVDAVVEQILPLLTPGDIIIDGG
                     NSFYKDTERRIKIVESKGVFYIGAGISGGEEGALNGPSIMPGGSKEAWPLVESILMKI
                     AAKADDGSPCCRWIGPGGSGHFVKMVHNGIEYADMQLIAEVYHVLKVVFNFDNEQIAK
                     YFTLYNKGKLKSFLMEITAEILTHRDNSGGYLVDKILDSAGQKGTGRWSVESAMELGV
                     PFNIIATSVFERILSTQIDDREKGEQVYGHQIHKVVNSTVEDIFKAMYCSRVALYSQG
                     FELLDMASKKFEWDLNLSSVAEIWRNGCIIRSAFLDDISAVFRQEESSNKLLMTSSFA
                     DDIIKLQDVWKNVVSEILSAGESIPVMSASLNYFLSTTSKNLPANLIQAQRDFFGAHT
                     FERVDRPRGEFFHEEWKRNQ"
     gene            14485..>15041
                     /locus_tag="P5293_02855"
     CDS             14485..>15041
                     /locus_tag="P5293_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRR48875.1"
                     /translation="MEEIVFVIFGGSGDLTRRKLIPALYNLYREKRYSYNVGIIGVGR
                     TQYSCQSYRDYLKDFLAKQIREDEWDEMSVDSFFLGVHYVIMDQTQESDYLILNSNIQ
                     NLFPACNNFIFYLATPPVLYEKIPLFLKSSGLISNSDKSGIRRVVVEKPFGYDLESAL
                     KLDIIFKKVFDEDSIYRIDHFLGKET"
BASE COUNT         4820 a         2253 c         3044 g         4924 t
ORIGIN      
        1 gattttgtcc acagtcgcgc tctttgttca actccaaacc tatgttgttc gtcaattaca
       61 gcgaatccca aattattaaa tttaactcta tcttcaataa gtgcatgtgt gccgaaaagt
      121 atatgaattt tgccttgaga aagtccctct aaaattttat ttctttcata agactttgtg
      181 cttccggtaa gcagggcaat ttcaattcct gatccttcaa caatttttaa tgcacttttg
      241 taatgttgat ttgcaagcac ttctgtcggg gccatgatgc aggactgaaa gccgttgtct
      301 gcagcaagca aagcacaaag aacagcaacc atagtttttc cgctgccgac atctccttga
      361 agtaatctgt tcatttgttt accgcttacc aggtcagacc ttatctcttt tataactctt
      421 ttttgtgcat tagtaagttc aaatggaata ttttggaaac aggcattaaa atattttcct
      481 actcgtggca tcataaatcc tgttgtaaat ctctctcgta aatttttttg acgtagaaga
      541 gaaagttgaa gataaagtag ctcttcaaac ttgattctga aaactgccag atttaatgat
      601 acttggttat caggaaaatg tatcattttt agggcatttc gaaggggaag cagtttttta
      661 ttttcaatta agtaggaagg gagtgtctct tttatggcat cacttgtcat ctttagaagg
      721 agagactggt atttagcaat ttgtttgttt gtgatgccac ttgactttag cctttcagta
      781 gaagaatata taccaatcat attgccgctg taaagagaat tctcttcatt gggagggtct
      841 atctcggggt gaataaagtt gattgactga ttgaaaatgg ttggtttacc aaatacaata
      901 tactctgagt taattttaat tttattcttg atccatttta ttccgttaaa aaataccagg
      961 tcaactacgc ctgtagcatc agccagcgta gccacaagtc ttttcttctt accaccttct
     1021 ccgatcagat ttattctgac aattcttcct cgaagttgta tatggctcat atcaggatca
     1081 atttccgata tggagtatat ttttgttctg tcaagatatc gaaatggaaa aaaatagagc
     1141 atttctctga aagttctaat gttcaattca gaggcaagaa gttctgctct tttagggcca
     1201 atacctggca gatactttat atcgctgtcg aggagatttg acatattcac aaatttaaga
     1261 atcttatcga tttttcgtat cttgcaataa atttacagaa acaagttgaa atgataaaca
     1321 ataaaatagt tgttggtatt tctcaaggag atatcaatag tatttcttca gaaataatta
     1381 taaaagctct ttccgattct cgattgctcg atacatgcat accagtaatt tatggttcct
     1441 caagggtaat aggaatttat aagaagcata gtcccgatac tgaaaatttt tccgtgaatg
     1501 taataaattc ttcaaaagat gctcacgcta aaagaattaa cataattaat tgtataccag
     1561 ataattaccc atttgagcct ggtaggccga gcgaagaggg agcaaaagct gccatgattg
     1621 ctcttgagaa gtctgtcaat gatcttaaaa ataaaaatat agatgtactt gttaccggtc
     1681 cttttaataa gtttgaagca aaacgtagtg gctttaattt cacaggtcat accgagtatc
     1741 ttgccaatgt atttgctgtc agtaatccac ttatgttcat gatttccgac agaatcaaag
     1801 tcggcttggt aacaaatcat atacctatag cccaggtatc atcctctata aacagcggac
     1861 taatcgttga gaagcttaga attatgtcgg cttcattgaa aagggatttt gcaatttcat
     1921 ctccaaagat tgctgttttg ggcttaaatc cacattgtgg tgatcaggga ttaataggga
     1981 atgaagagtc tgatattatt aaacctgcta ttcatcaggc ctttgatgaa ggtattctgt
     2041 cttttgggcc ttattctcct gatggttttt ttggaagtga gatgctctgc aaatttgatg
     2101 ctgtgttagc aatgtatcat gatcaaggtt taatcccttt caaaacaatt gcttttgaaa
     2161 acggtgtaaa ctttactgca ggtttgcctg ttgtgaggac atcacccgat catgggacag
     2221 catatgatat tgcaggaaaa aataaagcta atccagcttc gatgctttct gcaatatatt
     2281 gggcatgtga tattttcaaa aatagaaaat attatgacga gttgcgctcc aacgttcttt
     2341 cttcagatca atatatttct gagaaacagt aatgattgaa atttttactg atggtgcttc
     2401 caggggtaat cctggtcctg gtggatatgg tgtaattctt aaatcaggaa agcattataa
     2461 ggaactttct ggcggatttg catatactac aaacaatcga atggagttga tggctgttat
     2521 taaaggtttg gaagcaatca aggtacaaaa ggcagatatt tgcgtttata ctgactcatc
     2581 atacatttgt aacgcaatca accagggatg gctttttaag tgggagaaga aaaattttga
     2641 aaaaaaaatg aatgcagatc tttggaaaca atttcttcct ctttacagaa aacataatgt
     2701 caagtttata tggataaaag gacatgcagg tcatccggag aatgagcgat gtgacaggtt
     2761 ggcagtggag gcatctatga atccagatct tcctgtagac actggttata cttgtttacc
     2821 ggagtgtaat cctgattcct caaccaagga ttaaaaccgg cttctctgat agctttctgc
     2881 atctcttctg cattcatgga gttatttgca cctgctgacg agacgacatt ttcttcaatc
     2941 ataatggagc ccatatcatc agcaccacaa tgaagtgcaa tcattccggc ttgtttgccg
     3001 atagtgagcc atgacacttg gatatgatca acattgttga gaataattct ggaaattgct
     3061 accattttta gatgttcaat aagagaaagt tgtcttagct taaatttttc agccagtttt
     3121 gtgtctgctg tctgcatcgg ccaactgata aaagctctga aacccggtgc ttcagcaggt
     3181 tttctgtttt gaatatctct tagcaaaatc aaatgttcaa ttctttcctc gagagtttct
     3241 atatgcccat aaaccatagt agctgtagtg ccaatatgaa gtttgtgtgc ttcttccata
     3301 acttctatcc agctttcaac tccgggtttg gcaggagaaa ttatcttcct aactctgttt
     3361 gaaagaattt cagcacctgc acctggaaga gtatccagtc ctgcaagtga gagacgaaca
     3421 agtgtttctt tgatggagag attgctcaac tttgcaatat gtgcaatttc cggagggcct
     3481 aaagcattta gttttataag aggatcaatt gccttgattt ttctaaatag ttcttcataa
     3541 tatgaaatgt cgtattcagg gtgtaatcct ccttgcagaa gcagttggtc tccgccaagt
     3601 ttacgaagct cagttatttt ttcttcaagt tgactttcag taattgtaaa gctcttctct
     3661 ttttgtgaaa tgttgcagtg aaaattgcaa aagatacaac ctgatataca tacgtttgta
     3721 taattgacat ttctatcgat ctgccaacta acttttgact caggattatg aatatagcga
     3781 agtctttttg caagtgaaaa taattcattg acaggagccc tttgaaaaag ttccatgcct
     3841 tcactgcaat ttagtggctc aagatttaat gctttagtat acaggttgtc cagctccata
     3901 atgatttgta taatgcttag caaaagtatt taattatttc gtttatggct caaattgggt
     3961 atatttgttc tgttatggaa aaatatatag aaagaaaaaa ttcgaatttg gctgtcattg
     4021 gtgctggaag ctgggctacg gctttggcta aattacttct caacaattgt ggagaaatct
     4081 cctggtacat cagaaacaaa agggtactgg aatctattaa aacaaccggg cacaacccac
     4141 tctatcttga agatgtacac tttgacacat caaaactgaa aatcagtaac gatattaata
     4201 aaattgtaga tggcgctgat gtcttgcttt tttgtattcc ttctgcctat tttttgcctg
     4261 aagttgctaa aattcaaaat ttaaaggata aattcattat atctgcaatc aagggcatta
     4321 ttccgggaga taatattact attgctgaat attttcacag ggttcataaa attccattca
     4381 gcagaatcgg agtaattagt gggccttgcc acgctgagga aatagctttg gagagacttt
     4441 cttatcttac gctttcaagc aaatacagag aaactgcttt aaggctttgc aatctctttt
     4501 cgtgcaacta tataaaaaca attccaggca ctgatatttt tggggttgaa tacgctgctg
     4561 ttcttaagaa tatatatgcc gttgctgccg gagttgcaac tactcttgga tatggggata
     4621 attttatggc agtgcttgta acaaactcgt ttcacgagat gagacgtttt ttgcatgttg
     4681 ctcatcctga ctccgaaaga tctaccacta aaagtgccta tttgggagat cttcttgtga
     4741 catgttattc taaatttagc agaaacagaa catttggcac aatgataggc aaagggtatt
     4801 cagtaagaag cgcattactt gagactaaaa tggttgcgga gggttattat ggtactaagt
     4861 gcatctggga gttgaatcag aaatttaatg tggatatgcc agttgctgat gctatgtaca
     4921 gaattttata tgggagggag aatgctgctt ctgttatgaa agagcttacg tccaagctgg
     4981 aataatagta aaattaaata aattgtttct atggaaaagg cagtaaaaat taatttggaa
     5041 gcaattaatg gttttttaac aagagaagaa attattgatg caaaaaaacg tgcattagct
     5101 tcatttgaca cgcttatcaa tcgttctgga agaggtaatg attaccttgg atggcttgac
     5161 ttgccaacca atactgatga agctgttttg caaagaatag atcagatagt tgcactatgg
     5221 aaaaataaga tagaagttgt tgttgtaatt ggtataggtg gttcctattt aggagccaag
     5281 gcggctatag atgctttgtc tcattctttt ggtgcctatt tgggcaagcg aagttttcct
     5341 gtaattcttt ttgcaggtca gaatctatca gaagattata tggcggagtt gtttgatatt
     5401 atcgataaaa agagtgtagc ctgtgtggtt atatccaagt cagggacaac aacagaacct
     5461 gcagtggctt ttagaatgat aagaaaatct atggaagaaa ggtatggaaa ggtggaagct
     5521 gctaatagga ttgttgcaat tactgataaa agtcgtggtg ctctcaagga actcgccgac
     5581 agtgagggat atgaaacatt tattatacct gacgatgttg gaggacgcta ttcagtatta
     5641 actcctgtag gtttgcttcc aattgctctt gcgggatttg atattcggtc tctgttgcgc
     5701 ggagcttgtt tgatgcagaa aatatgtcat ttgagagatg ataacaatcc tgccattgaa
     5761 tatgccgcta taagaaatgc actttataac aagggatgga aaatagagat attagcaaat
     5821 tacaatccca aattacaata ttttgccgaa tggtggaaac agctttttgg cgagagtgaa
     5881 gggaaagatg gtaaggggat ttatcctgca tcagtaaatt atactaccga tcttcactca
     5941 atgggtcaat atatccagag tggtgagaga attctttttg aaagtgtcct cagtgtggaa
     6001 aatccctcaa gatgtgccgt aattcctgaa gatgccagga atatggatgg cttgaatttt
     6061 atagctgcta aaagaataga tgaatgtaac aaaatggcag aggccggaac tcgcatagcc
     6121 catgtcgacg gtggagttcc aaacattcgg attataattg gaaacatatc ggaatattca
     6181 cttggagctc ttttctattt ttttgaattt gcatgtggaa ttagtgcata catgatggga
     6241 atcaatccat ttgatcaacc aggtgttgaa gattacaaaa ataacatgtt tgccttgctg
     6301 gggaaacctg ggtatgagga gattgccgaa aagctcagaa gaagagtctg atggattttc
     6361 ttaattccat agcaactgcc ttttatgaaa gagaaaaagg tttgatttct gagtactgtt
     6421 ttgtttttcc aaatcgaaga gcttcgcttt tttttcagcg tgctttagca tctattataa
     6481 gggaaccggt attttctcct gttattgtta caattaacga tctttttgaa caactatcat
     6541 cgcttaaaca ggttgacaga atagaggcgc ttactgaatt atggactctc tacaactcta
     6601 tttcgaccaa aaaagagagc tttgacgaat ttatttattg gggggatata attctttcag
     6661 attttgatga tgttgataaa tatttagtcg atgcagggaa actttttgca aacattcaag
     6721 acctaaaaga gattgaaagt gactattcgt ttttgactca aagacagttg gacgccattc
     6781 atcagttttg gtataatttc tttcctgcag gaagcagtga aaacaaactg aaattcaagg
     6841 agatttggga aattctggct cctctttatt atcggtttac agaaaagtta tctatgagat
     6901 ctataggata tgagggaatg atctatagaa aagttgcttc tgaaattctg tcagaaagat
     6961 attctgaaaa ttcatcgttt aaaaaactct ttctcaatag aaagatagtt tttattggat
     7021 tcaatgctct taatccatgt gaaaagactt tgatggatct tcttaaaaga agaggaatgg
     7081 cagattttta ctgggattat gcgggagaaa tgataagtaa ttcagacaat ctttcttctt
     7141 tttttataaa agataatata aaacgctatc catcagaaat agagatagca ccatattcat
     7201 gcggcaagtg ctcatttgaa gttattggag taccttcgac cattggtcag gcgaagagag
     7261 tgatgaatat tcttaaagag gtgggaggag atattaatac ggcagtagtt ttacctgatg
     7321 aatcactttt aatgcctgtt ctgtattcaa tacctgaaga agtgaaggat gtcaatgtta
     7381 ccatgggata tcctttgaaa gagggaggaa tagtatcgct tgttgggaat attcttgaac
     7441 ttcagaaagg tgtttattat tacagaagag ttttgcaggt tctccagcac aattatattc
     7501 aagtaattgc cggtaacgag gctaattcat taattgaaag aatatatcaa ggcaacgtga
     7561 tatatgttgg tgctgaggaa tttaatgttc atccactttt tcgtttgata ttcaaagaga
     7621 caaatgatat atcatcttat cttcttgata ttcttgaatt cttaaataga tcagttcaat
     7681 tgaccaaaat tgaaaaggag tttatttttt caatttatac ttcagttatg aggctaaagg
     7741 atcttttacc tgaagtatct gcagatactt atgctaaaat actcagtcag attatagcta
     7801 catcaacaat acctttcaag ggagagccat tgagtggttt gcaaataatg ggagtacttg
     7861 aaacaaggtg tctggatttt gaaaatgtaa ttatctgttc aatgaatgaa ggaatttttc
     7921 ccaagagatc atttaacaat tcgtttattc cagtgactct tagaagaggc ttttcacttc
     7981 cggattttga ttacagtgac agagtatccg cttatttgtt ttatagattg atttcaagag
     8041 caaaaaaagt ttatatgttg tatgatacaa gaagtgaggg acttaaaagt ggtgaggtaa
     8101 gcagatatgt atatcagttg aagtatcatt tcggacttca attacaggag agtattgctg
     8161 tgcaaagagt tgaattacct tctatcagag aggtagtaat taataaggat gaagggacta
     8221 ttttgcagct taaaaaactt tattttcctt taggagaatc agctctttct gcaagtgcta
     8281 tcaacacata tattacatgc tctcttaagt tttattatcg ttatattaag ggttttaaag
     8341 agaatgaaaa tgttgctgaa ggggttgaag cagatacttt tgggtctatt tttcataata
     8401 caatagctga tttgtacaaa attttcaagg ggagaagagt aacttcggag atgttgaaag
     8461 agttaagcag agatagtgtc aaaattgaga aagctattga cgattcattt ttgaaattca
     8521 tgaatctaaa agaggtcaga ggtcgaaatt tacttacaaa aaaactgata ttgaaatacg
     8581 ttaaacaggt ttttattttt gatactgctg tagcaccttt tgaatatatt gattctgaaa
     8641 tggttgttaa tagagaagtc gaattaaaag atggcttttc actgcattta aaaggggtaa
     8701 tggacagact tgacaggatt gaggagggca agagaatagt tgactataag acgggatatg
     8761 gaaaaattcc aaaaaatatt gatctggcaa aacttttttc tgaaaatcat gataaaggag
     8821 acgaggtcgt ttttcaactg ttgttctaca tgctgatcat taatgataag gatgtttctt
     8881 tttttgaacc atatctgctc agaaacctgt ttaaaaaaga tcgatatctg cagaatgtta
     8941 atgaagatat gttgctttct ttcagctatc agctcgggga taaactttct gaactttttg
     9001 acataactgt tcctttcagg caaacttcca gagagcaaaa ctgtgagtat tgcccataca
     9061 gtaaaatttg ccaccgatat gggaaatctt aaaattttaa aagcctctgc tggatctggt
     9121 aagacatata atcttaccaa agaatatctt aatcttatag agaaagaaag agagatattt
     9181 tcacatattc ttgcagtgac ttttacaaat aaggcaactg aagagatgaa gcacagagta
     9241 ataaaggagt tgcataaaaa gtccttatct tatcctgttg caaaagagag attaatagaa
     9301 attttacata attattctaa ttttaatatt agcacaatag acaggttttt tcaacaaata
     9361 atgagatcat ttgcccgtga gataggtaaa agtggttctt ataatgtgga gcttgacgat
     9421 aatctcattt tagttgaggc tgttgaaatg atgcttcaag atctggacat tgaagagaac
     9481 gaacaattgc tcgactggct ggtaaaattt tcaattgatg ctattgagga tggaaagtca
     9541 tgggatataa aaaagggaat tattccattt gctcaacaga tgtttaagga agcttttcga
     9601 gcaattaaaa gtgattttaa ggattctgtt aacaacaaaa taactctgca ggaatttatc
     9661 tcaaatcttg atggtgtaat taagaaatat gaagatgaga tattaaatat tacagaagag
     9721 gtctttagag ttatggaaag atattctctc agctgtgaaa gttttaaggg gggaaccaga
     9781 tcaaagttta agttttatga caagacagca tcaggagatt actattctgt atcacctaat
     9841 tctttaatgg cttttacaga ggattttgac aattggtttg ctaagtcagt tattaaaagc
     9901 aaccctgttt tatttgattc aatcagtaat gcatacaatg aatctttaag agaactggtg
     9961 gaacagactg tttcactcca gaatgactgg atagaagcac actcggccat tcttgtgcga
    10021 agcagaatat atatacttgg tattttgata gatataaaca aaaacataca gagatactct
    10081 aaagaacata atattatgct tctttcagac actacggaaa ttctcaacaa tattatagat
    10141 ggcagtgact ctccttttat ttatgagaaa attggcagtc gtatagatca ctttatgctg
    10201 gatgaatttc aagatacttc tttgatgcag tggaacaatt tcaaaccctt aattaaaaac
    10261 agtcttgcat caggtaacag taatcttgtc gttggagatg ttaagcaaag tatctataga
    10321 tggagaggat cagactggtc attgcttaat gccgaaatat acaacgattt tggaagagat
    10381 gatattgaag aaagtgtttt ggaagaaaat tggagatcat acgaaaacat tgttaatttc
    10441 aataataagt tttttaaata cgctgcaaat caatgtgata ttctgatagg ggacagtatc
    10501 attgctcaga aaatttattc tgattgtgag cagaaagttt gtaaacagaa tattggcaat
    10561 agtggacatg tgaaaattaa atttattcaa aaagaagaaa aaaagtggaa agaaaatgtt
    10621 ttgaatgaat taccattgat agttttgaaa ttccgagaga atggttatca gttcaaagat
    10681 atagcttgtc ttgttagaac gaacaaagat ggtgtaagtg tagttaataa gcttgtggaa
    10741 aaaggattgc ctgttatttc caatgagtct cttctaatca catcttcacg aggagtaaga
    10801 aaagtaattt caatcctaag aggtttggta aacaaaaatg atttattcag caaggcaata
    10861 gctgaattgg aagggcttaa accagattat gatgatgaca ttttacgact tcctatatat
    10921 gaaatttgtg aaagagttgt tgaatcactt gaagatgaaa ttcaggagac agaccatcct
    10981 tatattcaag gttttatgga tgcggttctg gattttttgg caaataacag tcctgactta
    11041 aatctttttt tggaatggtt tgataaacaa tggtataaat tgtccatttc ttctcctgag
    11101 gagcagaatg ctatcagagt tatgacaatt cacaagtcca aggggttggg aattaaagca
    11161 gttattgtcc cgttttttga tcttgaatta gatcataccg gaaatccaat tatctggtgc
    11221 aaacctgata gggctccatt taattctatt ccatttcttc ctgtctctta caagaaggaa
    11281 ttggaagaca ccattttctc ggcagattat aagatggaga agcagagaag ttatatagac
    11341 aatttgaatc ttgcatatgt ggcatttaca agggcaagag aagaacttgt cgtctatgcg
    11401 ccactgccat cggagaaagg gacaaacgct tcacttgcca atattcttta taatttactt
    11461 aaagaggaat taaatcctga tttagaatgt aatttgggtg aatggtgtca cgaaagcaca
    11521 cctgaaaata gtagaactac aacttattat gacaattata actctgtgcc gccgggtgat
    11581 agactacatc tttcgctgag agcgggagaa ttctttgaca agcaaagcaa acgaaattat
    11641 ggtttgataa tgcacgatat tctttctgga gtttatactg aaaaagattt gctgccttgt
    11701 gttaaaaaag ctgttgcagc cggtgaaata agcgcagacg aagagataaa gattcttgat
    11761 tatctgacca gaataatgga gagtgtagaa gaaagacatt ggtttgactc ttcttatact
    11821 atttataatg aacttgagat tcttagcccc ggaggaagaa taagtcgtcc tgacagagtt
    11881 ttgattggag atgataaagc aattgttata gattttaagt tcggaaatgt taaggattct
    11941 tcttacataa aacaggttgc aaattataaa aaattgattg aaaagataac aggaaaatcc
    12001 tcagagggat atatctggta tttagaggat aatgatatag ttgaggtcaa atagtagtgt
    12061 tgtttttcaa ataactttca attgcctcaa acggtgcgtc ttttaaaatt actgtttttg
    12121 acttgtcaag aagataaaga gaaggagtgg gcctgaggtc ataaagagaa ttgttcatga
    12181 ttaccagatc tttgtcgcaa ccgttcagcc attttttatt tagaagtggt gcaaaagatt
    12241 tccaattctc gaaatctact ccggcataca ttgttaaaac tttgaccttt cctccgaaag
    12301 aagctataac ctgagatttt tccatgtttt ccattgttga tttgcaactt gggcagtcag
    12361 ggtcaaaaaa aatcaaaata agatattctg caggaatgtt ataaagagaa cctctttgtc
    12421 catctggata gatgaatgaa aaatctatag cttttgttcc aactctattt ctcattgcta
    12481 attcaagttg ttttgtcagt ttttccttaa tggcagaatc taatttgtct gacttgatat
    12541 attcttcaag aactcctata taaagctctt cgtttttgaa aattgaatta gggtgataaa
    12601 agcctttttc aaacatctcc tggagcgcca tcatcatttt cggatgaccc aatatggctc
    12661 tgttagccaa agctttaaca gatgcaagag ccatgtcgtg tggtacagtt gtaagatatt
    12721 caagataggc gctgaaatgt gtatttagtc cttgttgatt tacaagatag gaagtgtcac
    12781 tgaaattaac accatcccag aagtgtttaa ctatataaag atttctttcg taattatcag
    12841 taataattga aggaacttca atcattttaa gttcgccttt aagagcgttt tgttcattat
    12901 tattcacatt tttacaggaa gaaagaacta aaagaatccc cgatagtata ataagtcttt
    12961 ttttaaattc agtcatcaat attaaattta tatgttcaat tacaaattta taaacttgta
    13021 atgtttttaa ttgttaataa taaattaaaa tgatatttcc atgagaaaaa cagatattgg
    13081 actgattggg cttgccgtga tgggtgaaaa tctggtattg aaccttgaaa gtaaaggtta
    13141 cagtgtgtca gtctataatc gcacaaaagc ggtgacagat ggttttatta gaaatagggc
    13201 tatcgggaaa agtatttttt cttattatac tttatttgaa ttcataaatt ctatctctct
    13261 tccgaggaag ataattataa tggtaaaggc gggagaggcg gttgatgccg tagtagaaca
    13321 gatattgccg cttctaactc cgggagatat tattatagat ggaggtaact ctttctacaa
    13381 ggatacagaa agaagaatta aaatagtaga gtcaaaagga gtattttata taggagcggg
    13441 aatttccggt ggtgaagaag gggcacttaa tggtccttca attatgcctg gaggttccaa
    13501 agaggcatgg cctttagttg aatcaatatt aatgaaaatt gcagcgaagg cagatgatgg
    13561 ttctccatgt tgtcggtgga ttggtccggg tggttcgggt cattttgtaa agatggtcca
    13621 caatggtata gaatacgcag acatgcagct tattgcagag gtttatcacg ttttaaaagt
    13681 ggtgtttaat tttgataatg aacaaattgc taaatatttt acattatata ataaaggaaa
    13741 gttaaaaagt tttttaatgg aaattactgc tgaaatttta actcacagag ataattctgg
    13801 aggctattta gtggataaaa ttcttgattc ggcaggacaa aaagggacag gaagatggag
    13861 tgttgaaagt gccatggaac ttggagtacc ttttaatatt attgcaacat ctgtatttga
    13921 aagaatacta tcaacacaga tagatgacag agaaaaaggt gaacaagtat atggtcatca
    13981 aatacataaa gttgtaaata gtacagttga agatattttt aaagctatgt attgttcaag
    14041 agtggcttta tattcacagg gatttgagct tttagatatg gcatcaaaaa aatttgaatg
    14101 ggatcttaat ttaagttctg tggcagagat ttggagaaac ggatgcatca ttcgttcggc
    14161 ttttttggat gatatatctg ctgttttcag acaagaagag tcttctaata agttattaat
    14221 gacttcttcg tttgccgatg atatcataaa acttcaggat gtatggaaaa atgttgtttc
    14281 tgaaatcttg tctgccggtg aatcaattcc tgtaatgtca gcgtcgttaa actatttttt
    14341 gtcaacaact tcaaaaaact tgcctgccaa tttgatacag gctcaaagag atttttttgg
    14401 agctcataca tttgaacgtg ttgacaggcc aaggggcgag ttttttcatg aagagtggaa
    14461 gcgtaatcag tgattattat tggaatggaa gaaattgtat ttgtgatatt tggtgggtca
    14521 ggagatttaa ccagaagaaa acttatccct gctctatata atctttacag agagaaaaga
    14581 tattcttata atgtcggaat cattggtgtt ggacgaacac agtattcttg tcaaagttat
    14641 cgagattatt taaaagattt tttggcgaaa cagataagag aagatgaatg ggatgaaatg
    14701 agtgtagata gttttttctt aggagttcat tatgttatta tggatcaaac tcaagaatct
    14761 gattacctga tactcaattc caatattcag aatctttttc ctgcttgcaa taattttatt
    14821 ttctatttgg ctactcctcc tgttttatat gaaaagatac ctttattcct aaaatcctcc
    14881 ggtttgatta gtaattcaga caaaagcgga ataagaagag tagttgttga aaaacctttt
    14941 ggatatgatc ttgagtcggc gttaaagctt gacataatat tcaaaaaagt atttgatgaa
    15001 gatagtattt accgaataga tcactttctt ggcaaggaga c
//
LOCUS       DAOTQL010000026        14984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3837_length_14984_cov_5.349722, whole genome shotgun sequence.
ACCESSION   DAOTQL010000026 DAOTQL010000000
VERSION     DAOTQL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3837_length_14984_cov_5.349722"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..883)
                     /locus_tag="P5293_02860"
     CDS             complement(98..883)
                     /locus_tag="P5293_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRR48876.1"
                     /translation="MVSIAGSGHPGGSLGCTDLLTVLFNKEMIHDPKKWRRDGMGFDM
                     FFLSAGHLAPALYSQLARTGYFPLNELYELRKIGSRLQGHPSVERGLPGIHIASGSLG
                     QGLSVAIGAALAKKLRNEENFVYVLTGDGECEEGQIWEAALFGAHHRVDRLIAMTDWN
                     GQQIDGPVDKILNLGNLQKKWEEFGWDTLIVDGNNIEEVIRAFSEIKSLVGNGKPKML
                     LCKTVMGKGVDFMEGTHKWHGKAPNAEQTEKALNQLKETLGDF"
     gene            1047..2834
                     /gene="lepA"
                     /locus_tag="P5293_02865"
     CDS             1047..2834
                     /gene="lepA"
                     /locus_tag="P5293_02865"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRR48877.1"
                     /translation="MRHIRNFCIIAHIDHGKSTLADRLLEYTKTLNSREMGDQVLDSM
                     DLEKEKGITIKSHPVQMDYIYEGEKYTLNLIDTPGHVDFSYEVSRAIASCEGALLVVD
                     ATQGVQAQTISNLYLAFNHNLEIIPVLNKIDMEAAMIETVKDQIIELTGCKEEEIILA
                     SGKTGQGVPEILEAIINRVPPPVGDEDAPLQALIFDSVFNSFRGIIAYFKVQNGIIRK
                     GDKVKFFNTGKEYEADEVGILKLKMVPTEEIRTGNVGYIISGIKIASEVKVGDTITHR
                     DRPCSTSIAGFEEVKPMVFAGLYPVEADEYEDLRASLEKLQLNDASLVFEPESSLALG
                     FGFRGGFLGLLHMEIIQERLYREFNMDVITTVPNVSYKVHTTKGEIIDVHNPSGLPAP
                     TLINCIEEPYIRAQVISKSEYFGVIMKLCLDKRGTLVSQHFLTSERVELNFEMPLAEI
                     VFDFYDKLKSVSKGYASFDYHVTGYKPAELVKLDILLNSEPVDALSSLIHKDHAYDFG
                     RRMCEKLKELIPRQQFDIAIQAAIGAKIIARETVKAVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMRQIGNVEVPQSAFMAVLKLD"
     gene            complement(2920..4575)
                     /locus_tag="P5293_02870"
     CDS             complement(2920..4575)
                     /locus_tag="P5293_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48878.1"
                     /translation="MRRSLSTILSIAVIGFAMVSCSNPAKMAKESQLITTETTPKVLE
                     VVAGEISANYSINFPEGYFHPKAIMEIVPVLVYEGGEVSAPAFKLQGEKVTDNFKVIP
                     IAGAKSNHSVKFAYKPGMEVSHLELRVAIWNKDKKIDFPAAYKLADGANTTYMLVDKS
                     GKTVCAKDEYKKVLSEQKEAQILYPINSAVVKPKELTKAEIKEFENFLASVSKDEKRT
                     VKSTNIVAYASPDGPETLNAKLSANRASTAKEAFDKITQKTSVNVPVDVTSIDEDWEG
                     FQELVSQSSIPDKELILRVLSMYSDPAVREREIKNMSKVFKILADKILPELRRARFIT
                     SIDYTNYTDEELKELAKTNIDIMDVEALLYTASLVDDMNTKIRLLTKAGDKFKCDRAY
                     NNLAALYLDNGKETEAKAALAKVADKTSDFYYNNAGILALRNGDYETAKTMFAKSKLP
                     ESRQNMAIIDILEGRYNDAVNKLAGTGDPNEGLAYILTNQLDKASSVIKCNCARSSYL
                     RAIIAARQGKMSEVAKELKNVHEKDEVLKARSLKDIEFAKFRE"
     gene            complement(4641..5402)
                     /gene="kdsB"
                     /locus_tag="P5293_02875"
     CDS             complement(4641..5402)
                     /gene="kdsB"
                     /locus_tag="P5293_02875"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRR48879.1"
                     /translation="MKVIAIIPARYGSTRFPGKPLTLIDGKSMIRRVYERSSAVFEHV
                     CVATDDLRIFDEVKSFGGNAVMTSEKHRSGTDRCYEALQIVEKKDSAKFEVIVNVQGD
                     EPFIEPGQLLELMNCFNDSNTQIATLVKKFSPEEDIFTLNTPKVVVSKDMNALYFSRS
                     VIPFKRGIEKDKWNDGNTYYKHIGLYAFTREVFEQITKLEQSPLEVAEQLEQLRWLEN
                     GYKIKVAVTQYQSFSIDTPKDLEIVLSKNIIKKGE"
     gene            5448..>6267
                     /locus_tag="P5293_02880"
     CDS             5448..>6267
                     /locus_tag="P5293_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR48880.1"
                     /translation="MYIMDIRAIKQRFDIIGDNQALNRAIDIAVQVASTDLSVLVIGE
                     SGVGKEVFPQIIHTNSPRKHSQYFAVNCGAIPEGTIDSELFGHEKGSFTGAYEMRKGY
                     FEVADGGTLFLDEVVELPLSTQVRLLRVLQSGEFIKVGSSKVQKTDVRVIAATNVNIM
                     KAIETGRFREDLYYRLNTVPIYVPPLRERKEDIHLLFKKFALDFADKYKMPAIRLTPE
                     AVKVLESYRWPGNVRQLKSIAEQISILEQNREISSSVLRKYIPEEVITHLPVKAE"
     assembly_gap    6268..6367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6368..6577
                     /locus_tag="P5293_02885"
     CDS             <6368..6577
                     /locus_tag="P5293_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48881.1"
                     /translation="EVASQNRLTVTVKIKYTNIKHPEENFEKSFAAFEDYSSTLGLDV
                     VEASLVDSIVEKLVNEIFNATVANW"
     gene            6589..7218
                     /locus_tag="P5293_02890"
     CDS             6589..7218
                     /locus_tag="P5293_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48882.1"
                     /translation="MADANLSTLESTIEQYPWFALGHFELFRKACELGEEHVRSCLSR
                     TAAHIYSREILFNLVKRAREHNNSETQVVEEAFDEIVFEVEEEPLNIHLTSKIIMAGG
                     DYFSKEELEETSLDPHEPLDKFICEKPSLLKSSINSRLNIDVAEEKIAEEEFDDSGFY
                     TETLAKIYSEQGYYKKALEVYAKLILLYPEKSSYFASLAQEIKSKHNQN"
     gene            7220..7588
                     /gene="secG"
                     /locus_tag="P5293_02895"
     CDS             7220..7588
                     /gene="secG"
                     /locus_tag="P5293_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRR48883.1"
                     /translation="MYTAISILIVIASVLLTIIVLIQNSKGGGLAANFAAGNQTFGVR
                     QTADFLEKATWTLAISVIVLCILATAFVSTGGQEASTVQQKIENAAPIQGQPQFPSST
                     PSNTPAPNNAPAENQAVPQK"
     gene            7724..8290
                     /locus_tag="P5293_02900"
     CDS             7724..8290
                     /locus_tag="P5293_02900"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRR48884.1"
                     /translation="MKKVINVGIGGRSFVIDEDAYQQLEKYLDKFRDKIGMGIQTKDV
                     MEDLEQRIAELFSESLKSEQEVVNLSLVNKVISQLGMPDGSPVEEDFTSCVSGTPNSV
                     VTKRLYRDPDSRVIGGVCSGLAYYLNVDVVLVRVLFCIAFMLGGVGFWGYIIFWIVTP
                     LANTASKKCEMRGLSVTAENLRKFSIYK"
     gene            8300..8641
                     /locus_tag="P5293_02905"
     CDS             8300..8641
                     /locus_tag="P5293_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRR48885.1"
                     /translation="MSDINTDNIKSQMRKGVLEYCILSILDNHDAYASELISQLKEAS
                     MIVVEGTLYPLLTRQKNQGLLSYRWEESPQGPPRKYYSITAKGRALLAEMDGVWEELI
                     QTIALLRKNSN"
     gene            8644..10158
                     /locus_tag="P5293_02910"
     CDS             8644..10158
                     /locus_tag="P5293_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRR48886.1"
                     /translation="MKKVVKVSIGNLAFTIDEDGYELLKSYLGELRAHYRFKPSGNEI
                     IEGIEERMAELFIEKVGINSVVSLEIVKEVINILGRPEVIDEEVTEDFNERRDSSYRS
                     IPRRLFRDPDNKMIGGVCSGLAAYINTDPLLIRILFIIFFLGFSFLGTHVGGPIFMLL
                     LYFVLWIMIPEAKTVEQKCAMRGEGVDLNNIQRRVEYGASKFSEYVRNSSSDSKSTMT
                     KISKVVTKILAILFIIFAIIGILSLTLLFLGIEIMNGFFPINLFDFVEFGITNSFWMK
                     LLIILVLLLPLVWMFIVGIQTLAGSRTRRFRPGLTIFIIWAISVFSLGIVGTIASKPF
                     WGQATEEVSYALRGDLDTIYIQLESDRKMPSDNLLLDADHSSFNLLWVDESGKELVIF
                     PEIDIIRQSEESARELICETTTMSNTYGEALLKGRNMPQCVDVKDSLITIRSDIYSKN
                     TKWNGTTKEIKLFLPEKVKVIITKPVNFDFSHRYRSDFQWVGGCKHFHSHYRSY"
     gene            complement(10161..11351)
                     /gene="kbl"
                     /locus_tag="P5293_02915"
     CDS             complement(10161..11351)
                     /gene="kbl"
                     /locus_tag="P5293_02915"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRR48887.1"
                     /translation="MYNNFQSYLSQELQEIKSSGLYKDERVITTSQKAVIKVNTGQEV
                     LNFCSNNYLGLSNNPVLINAAKEALDKRGFGMSSVRFICGTQDLHIELERKISDFFGT
                     EDAILYASCFDANGGVFEPLLTEDDAIISDSLNHASIIDGVRLCKAQRYRYTNANMAE
                     LEECLNIAQSQRFRLIVTDGVFSMDGNVAPIGEIYKLADKYNAMVMVDESHSAGVVGK
                     TGRGTTELYDLKGKIEIITGTLGKAFGGAIGGFTTGKKEIVAMLRQRSRPYLFSNSLP
                     PMVVAAGIATFNMMSKTNELQDKLHSNTNYFFNKMQTAGFDIKPTQSAICAVMLYDAK
                     LAQLFASKMLDEGIYVTGFYYPVVPKDQARIRVQISAGHEPEHIDKCVSAFIKVGREL
                     NVIK"
     gene            11504..12139
                     /locus_tag="P5293_02920"
     CDS             11504..12139
                     /locus_tag="P5293_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRR48888.1"
                     /translation="MELKERIVESSIRLFLQRGCKSVTMDDIVEENGISKRTLYEIFE
                     DKNSLLMECLETVYARTKTITDHIFATSNNVFELIFRTYECQSDSGYHLRLSFLTELK
                     KYYPEVYEQLLAKFSKYHYENTIKFISRGQAEGLIMEQLDISIVSRTLITMSNIVIRD
                     DTYDNVNATKIEILKQTILLYIRGISTERGIKLLDNYLNNKQYDQKKNEKN"
     gene            12126..13466
                     /locus_tag="P5293_02925"
     CDS             12126..13466
                     /locus_tag="P5293_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR48889.1"
                     /translation="MKKIRVLMIGGALLLLCGNVAIAQTNISQNHDTLRLTIDQALEI
                     ALSDNPSIKIAEAELERVDYLKKESWNALLPDLNTTMQYTNNIYKQIFFSDFFPGGKM
                     EVGSTHSYSVTTNATLPIFAMGLYKNIELSGLEIKAALESARTTKLDLIQQVKNSFYG
                     IVMMKESLRVLEKSYKNASESAENIKMMYEQGLASEFDKIRSEVAVRNILPSLTEARN
                     GLELAKLQLKILLSLKMDLPIDVIGDFSYLEAEINNFNSNIPYNFEMNSSLRTMDIQL
                     EKLNKTFELVRSQRLPVLAGFGNYVIQKQSNDFSFQGVWPNSLMVGVSLKVPIFNKFS
                     TTLKEKETKIGIRQLELQKNLLENSLSLAAQNAINAMQRAKIQLQSDKEAVAQAIKGY
                     EIAKVRYKTGIGTVLELNDSEVSLTTSELNLNKTLYDFLKAKNEFEKVLGIEKL"
     gene            13481..14530
                     /locus_tag="P5293_02930"
     CDS             13481..14530
                     /locus_tag="P5293_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR48890.1"
                     /translation="MKKGSILLALSLLFLSYACSSDKQKKGADDATVVPEVVEVKVAE
                     TVKQMVPQQVEFTGNIEPFVKNNIASGASQRVEKIMVDVGSHVRKGQLLVQMESVNYS
                     QAKIQLENLKVNLARTEELYKAGGISQQQYDQIKTQVDVAEQNISNLYKNTQLFSPID
                     GVVTQKNYDNGDMVGAMPILVVMQIQPVKILINISEEYFPQVKIGTPVQIALDVYNGR
                     KYPGKVSLIYPTIDPTTRTFQVQVSIPNPNLDIRPGMFARATVDFGSKDRIVVPDKSV
                     IKQVGTNDKYVYVLDGDIVRYTKVDVGKRVGSIYEIKSGLELGQKVVVAGITRLVDQA
                     KVKVVDTNLDLNKNE"
     gene            14534..>14984
                     /locus_tag="P5293_02935"
     CDS             14534..>14984
                     /locus_tag="P5293_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR48891.1"
                     /translation="MKLYESAVKKPVTTALIFVAIVVLGLFSYSRLSIDLIPDIEMNM
                     ATVMTTYSGASAADIETNITKGLETSLSTVSKLKKISSVSKDNVSLITLEFEYGTNMD
                     EAVNDMRSVIDMQATYLPEEAEKPVIFKFSSEMMPIFFISARTDKSIQ"
BASE COUNT         4662 a         2566 c         2990 g         4666 t
ORIGIN      
        1 tctttcttcc aacttcaagc aatccggctc caaagccgga tcgtgtatct ttattgcctg
       61 tatttataaa aatctctttc atgttaattg gtttgaatta aaaatcgcct aatgtttctt
      121 taagctggtt tagagctttc tctgtttgtt ctgcatttgg agccttgcca tgccatttat
      181 gtgtgccttc catgaagtcg actcccttgc ccattactgt tttgcaaagt aacattttgg
      241 gttttccgtt accaactagc gattttatct ctgaaaaagc tcggatcacc tcttcaatat
      301 tattcccatc aacaatcaaa gtatcccatc caaactcttc ccattttttt tgaagatttc
      361 caaggtttag gattttgtct acagggccgt caatttgctg tccgttccag tcagtcattg
      421 caattaatct gtcgactctg tgatgggcac caaaaagtgc tgcttcccaa atttgtccct
      481 cttcacattc accatcgccg gttaatacat atacaaaatt ctcttcattt cgcagttttt
      541 ttgcgagggc tgcgccaatg gctacagaca atccttgtcc gagagagccg gaagcaatat
      601 gaatacctgg caatcctctt tcaaccgaag gatgtccctg aagtcttgat ccgatttttc
      661 gaagttcgta caactcattc agaggaaaat accccgttct tgcaagttga ctgtaaagag
      721 caggagcgag atgtcctgca gatagaaaaa acatatcaaa acccatcccg tcgcgacgcc
      781 attttttcgg atcatgaatc atttctttgt taaaaagtac agtcaaaaga tctgtacaac
      841 ctagcgaacc tccgggatgc cctgaccctg ctattgaaac cattcgtata atatccctcc
      901 gggcttgagt agcgatatcg tttaaattcg taacagttaa cattattata ggttaaataa
      961 tttcaaatta ttacaaagat aaagccaatc aaatgatttt atcaattatt tttatctttg
     1021 caaccagcga tactcttaat ttttatatgc ggcacatcag aaatttttgc ataattgcac
     1081 acatagacca cggtaaaagc actttggccg atcgtctcct tgaatatact aaaactctta
     1141 attcaaggga aatgggggat caagtgctag attcaatgga tcttgagaag gaaaaaggaa
     1201 ttactattaa gagtcatcct gtacagatgg attacattta tgagggtgag aaatatactc
     1261 ttaatctgat tgatactccc ggacatgtag atttttcata tgaagtttca agagccattg
     1321 catcctgtga aggagcactc cttgttgtag atgctactca gggagttcag gctcagacaa
     1381 tttccaatct ctatcttgcc tttaatcaca accttgaaat tattcctgta cttaataaaa
     1441 ttgatatgga agcagccatg attgagacag ttaaggatca gattattgag cttacaggct
     1501 gcaaggaaga agagattatt cttgcaagtg gcaaaactgg acagggagtt cccgagattc
     1561 ttgaagcaat cataaacagg gttcctcctc ctgtaggtga tgaagatgct cctttgcaag
     1621 cgcttatttt tgattctgta ttcaattctt tcaggggcat tattgcttat ttcaaggttc
     1681 agaacggaat tataagaaag ggtgataagg ttaaattttt taacaccggg aaagagtatg
     1741 aagctgacga agtaggaata ttgaaactca aaatggtccc gacagaagag ataagaactg
     1801 gtaatgttgg atatattatt tcaggcatta agatcgccag tgaggtcaaa gttggtgata
     1861 caattactca cagagacaga ccttgttcaa cttcaatagc tggttttgag gaggtaaaac
     1921 caatggtttt tgcagggctc tatcctgttg aggcagacga gtatgaggat ctaagagcgt
     1981 cacttgagaa gttgcagctt aatgatgctt ctcttgtctt cgaaccagag tcttcactgg
     2041 ctcttgggtt tggttttcga ggaggatttc tgggtcttct tcatatggag attatacagg
     2101 agagactcta cagagagttt aatatggatg ttattaccac cgtgcccaac gtttcctata
     2161 aagttcatac tacaaaaggt gaaataattg atgttcataa tccctcaggg ctaccagctc
     2221 caacattgat caattgtata gaagaaccat atattcgtgc tcaggttatc tccaaatcag
     2281 aatattttgg agtaattatg aaactttgtc ttgataaaag agggacactt gtaagtcaac
     2341 attttctcac aagtgagagg gttgagctta actttgaaat gcctttggct gagattgtgt
     2401 ttgattttta tgataaattg aagtctgtct ccaaagggta tgcctctttt gattatcatg
     2461 ttaccggtta taagccggcg gagcttgtca agttggatat acttcttaac tcagagccgg
     2521 tagatgctct ttcaagtttg attcacaaag atcatgcata tgattttgga aggagaatgt
     2581 gtgagaaact taaagagttg attccaagac aacaatttga tatcgcaatt caggctgcga
     2641 taggagccaa gattattgca agagaaacag ttaaggctgt cagaaaagat gtaactgcca
     2701 agtgttatgg tggtgatatt tcgcgaaaac gaaaacttct cgagaaacag aagaaaggca
     2761 aaaaaagaat gcgtcaaata ggaaatgtcg aagttcctca aagcgctttc atggctgtgt
     2821 tgaaactgga ttaaagaaaa caacgttgat attgaatata aaaacaggag gctgtctaaa
     2881 aagtagacag cctctctgat ttatcagcaa aattatatgt tattcacgga atttggcaaa
     2941 ctctatatcc ttaagtgagc gggcttttag aacttcgtcc ttctcatgaa cattcttaag
     3001 ttctttggca acttcagaca ttttcccctg acgggcagca atgattgcgc ggagatatga
     3061 agaacgtgca caattgcatt taatcacgct tgaagctttg tccagctgat ttgtaagtat
     3121 atatgccaga ccttcgttag gatcaccagt tcctgcaagc ttatttactg catcgttgta
     3181 cctgccttca aggatatcaa tgattgccat attttgcctt gattcaggca attttgattt
     3241 tgcaaacatt gtctttgctg tttcataatc tccatttctc aaagccagaa tccctgcatt
     3301 gttataataa aaatcgcttg ttttgtcagc aacctttgca agagcagcct tggcttctgt
     3361 ctctttgcca ttgtcgagat aaagggcggc cagattattg tatgcgcggt cgcatttaaa
     3421 tttatcaccc gcttttgtca ggagtctaat cttggtgttc atgtcatcga ccaaactggc
     3481 tgtataaagc aatgcttcaa catccattat atctatattg gttttagcca gttccttaag
     3541 ctcctcgtca gtatagtttg tgtaatctat acttgttata aatctagccc tgcgaagttc
     3601 cggaagtatt ttgtcggcta aaattttaaa tactttagac atgtttttaa tctctctctc
     3661 tctcactgca ggatcgctgt acattgatag tacacgcaaa ataagctctt tgtcaggaat
     3721 tgaagattgt gatactaatt cctggaatcc ttcccagtct tcgtcgatgg aagttacgtc
     3781 aacaggaaca tttactgaag ttttttgtgt aattttatca aaagcttcct ttgctgttga
     3841 tgccctatta gcagaaagtt ttgcatttaa agtttctggt ccgtccggag atgcataagc
     3901 tactatattt gtgcttttta cagttctttt ttcatccttg cttactgaag ctaggaaatt
     3961 ctcgaattct ttaatctcag cttttgtgag ttcttttggt ttaactactg cactattaat
     4021 cggataaagg atctgtgcct ctttttgctc gcttaaaact tttttatact catccttagc
     4081 gcacacagtc tttccggatt tgtcaacgag catataagta gtatttgcac catctgcaag
     4141 tttgtatgct gccgggaaat ctattttttt gtctttattc catatggcaa ctcgcagttc
     4201 aagatgagag acctccatgc ccggtttata ggcaaattta acgctgtggt ttgatttggc
     4261 accagctatc ggaataactt tgaaattgtc tgtaactttt tcaccctgaa gtttaaaggc
     4321 aggagcagaa acttcaccac cttcatatac aagtacgggt acaatctcca ttattgcttt
     4381 aggatgaaaa tatccttcag gaaagtttat tgaataattg gcactaatct ctccggcgac
     4441 cacttcaagt acttttggag ttgtctcggt agttatcagt tgtgactctt tggccatctt
     4501 tgcaggattg ctgcaggaca ccattgcaaa tccaattaca gcaatggaaa ggattgtaga
     4561 taaacttctt ctcatgttca ttattattgt atttaattta tttgtaattt tcgttataca
     4621 gtttacaaaa ttaataaatt ttattcacct ttcttgataa tattttttga taacacaatt
     4681 tctaaatctt ttggtgtatc aatagagaaa gattgatatt gtgtgactgc aactttaatc
     4741 ttataaccat tttcaagcca tcttaactgt tcaagttgtt ctgcaacctc aagaggtgac
     4801 tgctcaagtt ttgtaatttg ctcgaatacc tctctggtaa aagcataaag cccgatatgc
     4861 ttataatagg tatttccatc attccacttg tctttttcta ttcctctttt aaaagggata
     4921 acacttctac tgaaatacaa tgcgttcata tcttttgaca ctactacttt tggagtgtta
     4981 agcgtaaaaa tgtcttcttc gggcgaaaat ttctttacta aagtcgcaat ttgagtatta
     5041 ctatcattaa aacaattcat caactccaaa agttgtccgg gttctatgaa aggctcatct
     5101 ccctgcacat ttactattac ttcaaatttc gctgaatctt ttttttcaac aatttgtagg
     5161 gcctcgtaac aacggtcggt accactgcga tgcttttcag aggtcattac agcattcccg
     5221 ccaaaacttt tcacttcgtc aaaaatacgc aagtcatccg ttgcaacaca aacatgctca
     5281 aaaaccgcag agcttctttc gtatactctt cttatcatac tcttgccatc tatcaatgta
     5341 agaggcttgc cggggaaacg agttgaacca tatctggcag gtatgattgc tattactttc
     5401 atctaaatag attttaaatt tttcaaaggt acaaatttta actacttttg tatatcatgg
     5461 atatacgcgc aattaaacaa agatttgata ttattggtga taatcaagcc ctaaataggg
     5521 ctattgatat tgcagttcaa gtggcaagta cagatttgtc agttcttgtt ataggagaga
     5581 gcggggtggg caaggaggtt tttcctcaga ttattcatac aaacagccct agaaaacaca
     5641 gccagtactt tgccgtaaac tgcggcgcca tacccgaagg aactatagat tctgagcttt
     5701 ttggtcatga aaaaggatca tttactggag catatgaaat gcgcaaaggt tattttgaag
     5761 ttgctgacgg agggacactt tttctcgatg aggttgtaga gcttccttta tcaacgcagg
     5821 tcagactgct gagggtgctt cagtctggag agtttatcaa ggttggttcc tcaaaggttc
     5881 agaagacaga cgtaagggtt attgctgcta caaatgtcaa tattatgaaa gctatcgaaa
     5941 cagggaggtt tagagaagat ctttattaca gactgaatac tgttccgata tatgtacctc
     6001 ctcttagaga gagaaaagaa gatattcact tgttgttcaa aaaatttgcg cttgattttg
     6061 cagataaata caaaatgccc gcaatcagac ttactccgga agctgtgaaa gttttagaaa
     6121 gctacagatg gcctggcaat gtcagacaac taaagagtat tgccgagcaa atttctattt
     6181 tggaacaaaa cagagagatc tcttcaagcg ttcttagaaa gtatattcct gaagaggtaa
     6241 taactcattt acctgtaaaa gctgaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnngag gtggcttcac agaacaggct tacagttact gtaaagatta agtatacaaa
     6421 cataaagcat cctgaggaga attttgaaaa atcatttgca gcctttgaag attattcctc
     6481 tactcttggt ctggatgtag tagaggcttc tcttgttgat tcaatagttg agaagttggt
     6541 taatgaaatt tttaatgcca ctgtggccaa ttggtaaaat gattcattat ggcagatgca
     6601 aatttatcaa cattagaaag tacaatagag caatatccat ggtttgcact aggtcatttt
     6661 gaacttttcc gaaaagcttg tgaattgggc gaagagcatg ttagatcttg cttaagccgt
     6721 acagcagctc atatctattc gcgggagata ctcttcaatc tcgttaaaag ggcaagagag
     6781 cataacaact cagaaactca agtggttgaa gaagcttttg atgaaatcgt ttttgaggtt
     6841 gaggaggaac cacttaatat acatttaact tccaaaatta ttatggctgg aggagattac
     6901 ttttcaaagg aagaacttga agaaacttct cttgatcctc atgaaccact tgataaattt
     6961 atttgtgaaa agccatcctt gcttaagagt agtattaatt cccgtttgaa tatcgatgtg
     7021 gccgaagaga aaattgctga ggaagagttt gacgattcag gcttttacac tgaaacactt
     7081 gcaaaaatat acagtgaaca agggtattac aaaaaagcgt tggaggttta tgctaaactt
     7141 attttgttat atccggaaaa aagtagttac tttgcgtccc ttgcacagga aataaaaagt
     7201 aaacataatc aaaattgaca tgtatacagc aatctccatt ttaattgtga tcgccagtgt
     7261 tttactgaca ataatagttt tgatacaaaa ctcaaaaggc ggaggtcttg cagcaaactt
     7321 tgccgcgggc aatcaaacat ttggagttcg tcaaactgca gatttccttg aaaaagctac
     7381 gtggacactt gctatttcag tgatagtttt atgtatactt gctactgcct ttgtttctac
     7441 aggcggccaa gaggcaagta cagttcagca gaagattgaa aatgctgctc ctatacaggg
     7501 acaaccacag ttcccgtcct caactccttc aaatacgcct gcgcccaata atgcaccggc
     7561 tgaaaaccaa gcagttcctc agaaataatt ttacaaaagt gaacattcca ggtatctgaa
     7621 aagatacctt tttttaaaaa aacactacta tgcgatacaa ggtaataaaa attttatata
     7681 tatttgcact gccaaaaact attaaatttt aaatactata aaaatgaaga aagttataaa
     7741 tgtcggtatt ggtggcagaa gttttgtcat tgacgaagat gcttatcaac agctagaaaa
     7801 gtatttggat aaattccgag ataaaatcgg gatgggtatt caaacaaagg atgtcatgga
     7861 agatctcgag cagagaattg ctgaattatt ttcagaatct cttaaatccg aacaggaggt
     7921 tgtaaatcta tctttagtaa acaaggtgat ttctcaattg gggatgcctg atggtagtcc
     7981 tgttgaggag gatttcactt cttgtgtatc gggaacccca aattcggttg tgacaaagag
     8041 actttacaga gaccctgatt ccagagttat tggaggagtt tgtagtggtc ttgcttatta
     8101 tctcaatgta gatgtagtat tagtcagggt gcttttttgt attgccttta tgcttggagg
     8161 agttggcttt tggggctata ttattttctg gattgttacc cctttggcta atacagcatc
     8221 taaaaagtgt gaaatgagag gattatcggt aactgccgaa aatctgagaa agttttcaat
     8281 ttataaataa ttaaagatca tgagtgatat aaatacagac aatattaagt cacaaatgcg
     8341 aaaaggagta ctggaatatt gtatcctgag cattcttgac aatcatgatg cctatgcatc
     8401 agaattgata agtcaactta aggaagcgag tatgatagta gttgaaggta cactgtatcc
     8461 acttttaact cggcaaaaga atcaaggact tttgagctac cgttgggagg aatctcctca
     8521 aggtcctcca agaaaatatt attccataac tgctaaagga agggctcttc tggcagagat
     8581 ggatggtgtc tgggaggaat taattcaaac aatcgcatta ttaagaaaaa acagtaatta
     8641 gccatgaaaa aggtcgttaa agtaagtata ggaaatcttg cctttaccat tgatgaagat
     8701 ggttatgagt tgttaaaatc ctatttggga gagctgagag ctcattatag atttaaacca
     8761 agcggaaatg aaattataga aggcatagaa gagaggatgg ccgagctttt tattgaaaaa
     8821 gtcggtataa acagtgtagt ttcgttagag atagtcaaag aggtgattaa tattcttgga
     8881 aggccagaag ttattgatga agaagtcaca gaagatttta atgaaagaag ggactcgtct
     8941 tacagatcta ttccaagaag actttttcgt gatccggaca ataaaatgat tggcggagtc
     9001 tgttccggtc ttgcagctta cataaataca gatccgcttt taatcaggat attgttcata
     9061 atatttttcc ttggtttttc attccttggc actcacgttg gtggcccaat atttatgcta
     9121 ttgctatatt ttgttttatg gataatgatt cctgaagcaa aaacagttga acagaaatgt
     9181 gcaatgagag gcgagggggt tgaccttaac aatattcaga gaagagtaga atatggtgct
     9241 tcaaaattca gtgagtatgt tagaaattca tcttcagaca gtaaatcaac aatgacaaaa
     9301 atatcgaagg ttgtcacaaa gatattggct attttgttta taatttttgc aataatcggt
     9361 atattatctt taactcttct tttccttggc atcgagataa tgaatggctt cttcccgata
     9421 aatctatttg attttgtgga gtttggaata actaattcat tttggatgaa gcttttaata
     9481 atattagtgc ttttactccc tttagtttgg atgttcattg tgggtataca gacacttgca
     9541 ggatcaagaa ccagaagatt tagaccagga ctcaccatat tcattatctg ggcaatatct
     9601 gttttttcac ttggaatagt cggtactata gcttcaaaac ctttttgggg tcaggcgacc
     9661 gaagaagttt catatgcttt aagaggtgat ttggatacga tttatattca acttgaatct
     9721 gatagaaaga tgccgagtga taatttatta cttgatgctg atcactctag ttttaatctg
     9781 ctttgggttg atgaatcagg aaaagagttg gtaatttttc ctgaaattga cattataaga
     9841 cagagtgaag agtctgcaag agaattgatt tgcgagacaa ctacaatgtc aaatacttat
     9901 ggcgaagcat tgcttaaggg gagaaacatg cctcagtgtg tagatgttaa agattcactt
     9961 atcactataa gaagcgatat atacagtaaa aacaccaagt ggaatggaac tacaaaggaa
    10021 attaaactat tcctgcctga aaaagtaaaa gtaattatta caaagccggt taattttgac
    10081 ttttcgcaca gatacagaag tgattttcaa tgggtgggag gttgtaaaca tttccacagt
    10141 cactaccgaa gctactaatg ttacttgata acgttaagtt ccctgccaac ttttataaat
    10201 gctgaaacgc atttgtcaat atgttcaggc tcgtgacctg ccgaaatctg aacccttatg
    10261 cgagcctgat cctttgggac aacaggataa tagaatccag taacataaat tccttcatcg
    10321 agcatctttg aagcaaatag ttgagcgagt ttggcatcat agagcatgac ggcacaaatg
    10381 gctgactgag ttggtttgat gtcaaaaccc gcagtttgca ttttattgaa aaagtagttg
    10441 gtgttgctat gcagcttgtc ttggagctcg ttggtctttg acatcatgtt aaatgttgca
    10501 attcctgccg caacgaccat tggagggagt gagtttgaga aaagatatgg tcttgagcgt
    10561 tggcgaagca tggctactat ctcctttttg ccagtagtga agccgcctat tgctcctcca
    10621 aatgccttgc caagagtgcc ggtgattatt tctatcttcc ctttgagatc atacaactct
    10681 gtagtacctc ttccagtctt gccaacaact ccggcagagt gtgattcatc aaccattacc
    10741 atggcattgt atttatctgc cagtttgtat atttcaccaa ttggtgcaac attgccgtcc
    10801 attgaaaaga cgccgtcagt gactataagt ctgaatcttt gtgattgtgc aatattaaga
    10861 cattcttcca gctcagccat gttggcattt gtatatctgt atctctgtgc tttacaaagt
    10921 cttacaccat ctataataga tgcgtggtta agtgaatcgg atataattgc atcgtcttct
    10981 gtaagaagag gttcaaatac tccaccattg gcatcgaaac aagaggcata taaaattgca
    11041 tcctctgtgc caaaaaaatc agaaattttt ctttccagtt cgatatgcag gtcttgtgtt
    11101 ccacaaatga aacgtacact tgacattcca aatcctcttt tgtcgagggc ctctttggca
    11161 gcatttatga ggacaggatt attggaaaga ccaagataat tatttgagca gaaattgaga
    11221 acctcctgtc ctgtattaac tttgataaca gctttttgag atgttgtaat tactctttca
    11281 tccttataaa ggcctgaaga tttgatttct tgtaattctt gtgacaaata gctctgaaaa
    11341 ttgttgtaca tattgttata attagatgat tgcgacaaag tactggttac tattacttac
    11401 aaagttataa tgaaatctaa tattttataa aaaatatgaa aactattttt gttttttaag
    11461 tttccaattg taaatttgcg cctcagtttt gcgtttgttt aatatggaat taaaagagag
    11521 gattgtagaa agttctatca ggcttttttt gcagcgtgga tgcaagtctg tcacaatgga
    11581 tgatatagtt gaagaaaacg ggatatcaaa aagaactttg tatgaaattt tcgaagataa
    11641 gaattcgtta ttgatggaat gtctggaaac agtttatgca aggacaaaaa ctattacaga
    11701 ccacatattt gcaacatcca ataatgtatt tgaacttatt ttcaggacat atgaatgcca
    11761 atcggacagc gggtatcatt tgagattgtc ttttttaact gagttaaaaa aatactatcc
    11821 ggaggtatat gaacaattat tggcaaagtt ttcgaagtat cattacgaga atactatcaa
    11881 gtttataagt agaggacagg cagagggatt aattatggag caactggata taagcatagt
    11941 ttcgcggacg ttgattacaa tgtccaatat agtaatcagg gatgatacat atgataacgt
    12001 taatgcaacc aaaatagaga tattaaagca gacaatattg ctttatatta gaggaatatc
    12061 aacagaaaga ggtataaaat tattagataa ttatttaaat aataaacaat atgatcagaa
    12121 gaaaaatgaa aaaaattaga gtattaatga ttggaggggc actgttgctc ttatgtggca
    12181 acgttgccat agctcagaca aatatcagtc agaatcatga tacgttaagg cttactattg
    12241 atcaagcttt ggaaatagcg cttagtgata atcccagcat caaaattgca gaggcagagt
    12301 tagaaagggt agattatctt aagaaagaaa gttggaatgc ccttcttcct gacttgaaca
    12361 caacaatgca gtacacaaat aatatttaca agcagatatt tttctctgat tttttcccag
    12421 gaggtaagat ggaggtgggt tcaacacaca gttattcagt taccacaaat gctactttac
    12481 cgatatttgc catgggtcta tacaaaaaca ttgaactttc agggttggag ataaaagctg
    12541 ctcttgaatc tgccaggaca acaaaactag atcttataca gcaggttaaa aatagttttt
    12601 atggcattgt tatgatgaaa gagtctctga gagtcctgga aaaaagttat aaaaatgctt
    12661 cggagagcgc agagaacatt aagatgatgt atgaacaggg tttagcaagt gagtttgata
    12721 agatacgctc ggaagttgca gtaagaaata ttttgccctc tcttaccgaa gcaagaaatg
    12781 gacttgaact tgctaagtta cagcttaaaa tacttctttc attgaaaatg gaccttccaa
    12841 ttgatgtaat tggcgatttt tcatatctgg aggcagagat aaataatttc aacagtaata
    12901 ttccatacaa ttttgagatg aacagcagtt taagaacaat ggacattcag cttgaaaagc
    12961 tgaacaagac ctttgaactc gttcgttctc aaagactgcc ggttctagcc ggtttcggca
    13021 actatgttat tcaaaaacag agtaatgatt tcagctttca gggagtatgg ccaaattctt
    13081 tgatggtcgg agtatctctc aaagtgccaa tttttaataa gttttctaca actcttaagg
    13141 agaaagagac aaaaatagga attaggcagt tggaacttca aaaaaatctt ttggaaaact
    13201 ctctctcatt agctgctcag aatgccatca atgcaatgca gagagcaaaa attcagcttc
    13261 aatctgataa ggaggctgtt gcacaggcta taaaaggata tgaaatagcg aaggtgaggt
    13321 ataaaactgg gataggcacg gttctggaac tgaatgactc agaagtgtct ttaacaacat
    13381 cagaacttaa ccttaacaaa acgctatatg actttttgaa ggctaaaaat gaatttgaaa
    13441 aagttttggg gattgaaaaa ttataaataa taaaacacat atgaaaaagg gatctatttt
    13501 attggcttta agcttattat ttttgtcata cgcttgctct tctgacaaac aaaaaaaagg
    13561 tgcagacgac gctactgtag tacctgaagt ggtggaggta aaggtcgctg aaactgtaaa
    13621 acaaatggtt cctcagcaag tggagtttac cggtaatatt gaaccatttg taaaaaacaa
    13681 tattgcaagt ggagcctcac aaagagttga aaagataatg gtagatgttg gctcacatgt
    13741 caggaaagga cagctgcttg ttcagatgga gagtgttaac tattcacagg ccaaaattca
    13801 actcgagaac ttaaaagtta atctggcacg tacagaagaa ctctacaaag cgggaggtat
    13861 ttcacaacag cagtatgatc agataaagac tcaggtggat gttgctgaac agaatattag
    13921 taacctttat aaaaatactc aacttttttc tccaattgat ggagttgtaa cacaaaagaa
    13981 ttatgacaac ggagacatgg taggagccat gcctatactg gttgtcatgc agatacagcc
    14041 tgtaaagatt ttgataaata tttcagaaga atactttcca caggttaaga ttggaactcc
    14101 tgttcaaata gctcttgatg tatataatgg aaggaagtac cccgggaaag tcagtcttat
    14161 ttatcctaca attgatccca caacgagaac atttcaggta caggtcagta ttccaaatcc
    14221 caatttggac attcgcccgg gtatgtttgc tcgtgctacg gtagattttg gctccaaaga
    14281 tagaattgta gttcctgaca agtcagtaat taaacaggtg ggaacaaatg acaaatatgt
    14341 atatgttctc gatggggata ttgtaaggta tacaaaagta gatgtaggca agagggttgg
    14401 ttctatttat gagataaaaa gtggtttgga acttggtcaa aaagtggttg tagcaggtat
    14461 taccaggctt gtggatcaag ctaaagttaa agttgttgat acaaatcttg accttaataa
    14521 aaacgagtaa cttatgaaat tatacgaaag tgcagttaag aaaccggtca caacggcact
    14581 catttttgtg gcgattgtgg tattgggact tttttcatat tcaaggcttt ctatagatct
    14641 tattcctgat attgaaatga acatggctac agtcatgact acttattcag gagcaagtgc
    14701 agcagatata gaaactaata ttacaaaggg acttgaaaca tctttgagta ctgtctccaa
    14761 attgaagaag ataagttctg tgtcaaaaga taatgtgtca ctcatcacac ttgagtttga
    14821 gtatggcacg aacatggatg aggcggtgaa tgacatgaga tccgttattg acatgcaagc
    14881 cacctatttg ccggaagagg ctgaaaagcc ggttattttt aaattcagtt cagagatgat
    14941 gcctatcttc tttatctcag ccagaacaga caaaagcatt cagg
//
LOCUS       DAOTQL010000027        14803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3891_length_14803_cov_5.559330, whole genome shotgun sequence.
ACCESSION   DAOTQL010000027 DAOTQL010000000
VERSION     DAOTQL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3891_length_14803_cov_5.559330"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..817
                     /locus_tag="P5293_02940"
     CDS             281..817
                     /locus_tag="P5293_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRR48892.1"
                     /translation="MNIYLQIFSTFSKIGAFTIGGGYAMLPLIQREVVTNKKWLSEED
                     FIDVLAISQSTPGVFVINISVFIGLRIKGIKGSIVAALGSALPSFVIILLIAMFFNSF
                     KDNEIVEKIFKGIRPAVVALIAVPLIGMSKNMNLNRYTAFMPVITVMLIVFFQISPIY
                     ILIAGAIFGIFYPYLFKK"
     gene            814..1338
                     /locus_tag="P5293_02945"
     CDS             814..1338
                     /locus_tag="P5293_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRR48893.1"
                     /translation="MIYLQLFITFFKIGLFTFGGGYAMISLIQNEVVVNHHWVDAASF
                     TDMIAISQMTPGPIGINSATYVGYSVTGNAFGSAIATIAVSLPSFIIILTIAHFYVQF
                     KKNVLFESAMNGIKPVVLGLIGAAATLLVTKETFIDWKSWIIFGIAFALTQWGKFNPI
                     YVILLAGLAGYLIY"
     gene            1362..2024
                     /locus_tag="P5293_02950"
     CDS             1362..2024
                     /locus_tag="P5293_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRR48894.1"
                     /translation="MKKLLITIIVLTSTFFTSLGQEPKYTPLYYHRSSLFESLPINKS
                     DIIFLGNSIIHYCEWAELFDNPHVKNRGISGDIVQGVYDRLDPIIKGQPKKIFIMIGV
                     NNLSRDYTVDSTYMGLVKIVDKLSKECPYTKLYIQSALPVNSTFSNYSKHRTRGEEIK
                     KYNTMVEKMCQEKGITFINLYPLFKCKDSDELDPEFTRDGLHINGDGYLVWRDAIKKY
                     VK"
     gene            complement(2300..2375)
                     /locus_tag="P5293_02955"
     tRNA            complement(2300..2375)
                     /locus_tag="P5293_02955"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2338..2340),aa:His,seq:gtg)
     gene            2558..4339
                     /locus_tag="P5293_02960"
     CDS             2558..4339
                     /locus_tag="P5293_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HRR48895.1"
                     /translation="MAREIKFSLLYRDMWQSSGKYVPMVHQLKQIAPVIIEMGCFDRV
                     ETNGGAFEQVNLLFGENPNKAVREWTAPFNKAGIQTHMLERGLNALRMNPVPADVREL
                     MYKVKAKQGTNISRSFCGLNDPRNLELSVKYAKSGGMISQVALSITHSPVHTVEYYMD
                     LVDKVVEFGADEICLKDMAGIGRPAFLGRLTKSIKDKYPKMIVQYHGHSGPGFSVASM
                     LEVARAGADYIDVAMEPLSWGMVHPDVITIQAMLKDAGFLVKDINMNAYMEARSLTQS
                     FIDDYLGYFIDPGNKVSTSLLVGCGLPGGMMGSMMADLKGFQGAINVSLRNQDKKELK
                     LDELVVMLFQEVEYVWPRLGYPPLVTPFSQYVKNTALMNLMHILKGEPRWSTIDKDTW
                     NMILGKTGKLPGELAPEIKQLAKERGMEFYDGNPQDAFPNELDRFRKEMKENNWDFGE
                     DDEELFEFAMHERQYRDYKSGVAKQRFNAELEAARAKANAPIVVARPVVELPKVDVEK
                     ILATYPNAKPVQSTVKGQLIWQYDMIDKSSAPNIGEVVKAGKPMCFVQAFYGLEAIKP
                     AFDGKIVAICAKHGETVQKGEIIAFIE"
     gene            complement(4659..4732)
                     /locus_tag="P5293_02965"
     tRNA            complement(4659..4732)
                     /locus_tag="P5293_02965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4696..4698),aa:Arg,seq:ccg)
     gene            4809..5684
                     /gene="fabD"
                     /locus_tag="P5293_02970"
     CDS             4809..5684
                     /gene="fabD"
                     /locus_tag="P5293_02970"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRR48896.1"
                     /translation="MKAIVFPGQGAQFSGMGKELYNSYPEARELFERANEILGFRISE
                     IMFEGSDEALKQTKVTQPAVFIHSVILSICHKDLMPHMVAGHSLGEFSALVAAKAISF
                     EDGLKLVAARASAMQKACELEPSTMAAILGLDDEIVEQVCSSIQGIVVPANYNTKGQL
                     VISGSVDAVAKACEALKAAGAKRALPLAVGGAFHSPLMEPARKELAEAIAVTYFNKPA
                     CPIYQNVDAKPHTSPEEIKENLISQLTSPVRWRQSIENMWADGAREFIELGPGNVLQN
                     MIKKILPESTTGGYQ"
     gene            complement(5689..6414)
                     /locus_tag="P5293_02975"
     CDS             complement(5689..6414)
                     /locus_tag="P5293_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48897.1"
                     /translation="MKDEWNIKRIVANTCLIAIILSLSVRIGFAQDGILKVQNGKIAD
                     SELFKKNRYAMPSFSKGIIMLKDRRSYSGIFNIDNCSQTLRMIDEKGDTVTVAAEDMV
                     VSLSAGGYLFLKIKNNYVQILDTDGETSLGVVRQITFGRKVQEGVFGITNDIASMNNI
                     VSTEDNFYYQIEKGLWSGSVPFNYKEHVFLVKDGKLFPVNKPTLKKFYPKKYPEIENY
                     ISEHNPDLSKREDVSLLYKFVIR"
     gene            6719..10249
                     /gene="nifJ"
                     /locus_tag="P5293_02980"
     CDS             6719..10249
                     /gene="nifJ"
                     /locus_tag="P5293_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRR48898.1"
                     /translation="MAKEKKFITCDGNYAAAHVAYLFSELAAIYPITPSSPMAEYVDE
                     WSAFGKTNLFGEKVKVVEMQSEGGAAGALHGALQAGVLATTFTASQGLLLMIPNMYKI
                     AAELLPTVFHVSARTLATHALSIFGDHSDVMATRQTGFAMIASSSVQEIMDLGAVAHL
                     STLKSKIPFVHFFDGFRTSHEIQKIEMLDQEALKAMISEKSLIAFRNKALNPVRPVTR
                     GTAQNPDVFFQAREACNPFYDAVPDIVARYMGEISELTGRHYLPFDYYGDPEAENIII
                     AMGSVCETIKESVDYLTEKGEKVGLLTVRLYRPFSAKYFFRALPKSVKKIAVLDRTKE
                     PGSTGEPLYLDIKSVIADQGVKMPLIIGGRYGLSSKDTSPAQILSVFENLKLKEPKNG
                     FTIGIVDDISFKSLPVKEEISLAKSGVKECKFYGLGSDGTVGANKNTIKIIGETTPKY
                     VQAYFDYDSKKSGGYTCSHLRFGDSPIQSPYLVSTPDFVACHVTSYLHKYDILKGIKK
                     GGTLLLNTLWSVEETKEKLPDYIKATIAKKDLKLYIINATNIAEEIGLGNRTNTILQS
                     AFFKITNIIPYDQAVKEMKKAVEKSYGRKGEAILKMNYDAIDRGAEYVQVDIPCEWKN
                     LTAKFRPSNYDRLAPDWVRNVADPVNAQNGNDLPVSAFLNLEDGTIPAGTAAYEKRGI
                     AVNVPEWQSENCIQCNQCAYVCPHAAIRPFLMSEEEQANAPKGIKKLTGNGPFKAYSF
                     TIQVSVMDCTGCGNCVDICPAKNQAIIMKPLETQLHEQSNFDYLNSVVGYKDTIQPKT
                     QNVKNLMFSQPLFEFSGACAGCGETPYLKAISQLFGDRMMVANATGCSSIYSGSFPSS
                     PYTTNKDGYGPSWANSLFEDNAEFGLGMRLGSERVRDRIAKLMADGLECDKCSAETKM
                     LFGEWLSKRNDPAATREIADKLVPLITSCNCDICKELTTLKDFLVVRSQWIVGGDGWA
                     YDIGYGGLDHVLASGENVNVIVLDTEVYSNTGGQSSKSTPAGAVAKFATSGKKIRKKD
                     LGMMAISYGYVYVAQVAMGANNNQLLKALKEAEEYNGPSLIIAYSPCINHGLKNGMGA
                     SQREEKLAVECGYWHLYRYNPALEAEGKNPFTLDSKEPDWSKFQDFIKGEVRYSSLVK
                     TFPKEAEELFKLTEEYAKMRYNSYKRLASI"
     gene            complement(10318..11745)
                     /locus_tag="P5293_02985"
     CDS             complement(10318..11745)
                     /locus_tag="P5293_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48899.1"
                     /translation="MAKERAAMGFRENEIENIGKSLSWYQSHRKDGRNYCRALLYNGI
                     LISMGGSKDSTAYDYLIKADSVYQAYSLEDPYILSKCYEYKSKILRAQKAFPLAEVYL
                     NKAIKAASELNDSVGIVRLKTKLLRLYLLMGNIPKAFQSIASIQFAQNASPAMEYELC
                     NAMYIFYVSMRESEIATAYLKRMLNLAKKYNLKDVDYQSLNYRLAIYYDNKGIRDSAE
                     TYFLETLKQSNDTVADKSYTYPARYALFLQKHGDFQSACNYYKEAYENLLRYSTTRTR
                     EKLLEIDNKNEVAKLNTQLDKAEKHQLYLILVVVVLFFFLTYNVLKNNYLRKSNISFA
                     GIEEKEWLLSEISKANALSMPDLIEDVFNEAERSRRYNQTISDNLHKFVKRTKEEMKD
                     QYSQIVNSNRFISAYPYTRYLSSICSPIDILMLVLVKSGLTNKDISIILSIPYSSVRA
                     RKSKIKDVILESGDIPEEEKKIILF"
     gene            complement(12004..13710)
                     /gene="ftcD"
                     /locus_tag="P5293_02990"
     CDS             complement(12004..13710)
                     /gene="ftcD"
                     /locus_tag="P5293_02990"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRR48900.1"
                     /translation="MIKKLIECVPNFSEGRNMSVIKQITDEIESVEGVKLLNVDPGAA
                     TNRTVVTFVGAPEAVVEAAFRAIKKASEVIDMSRHHGEHPRMGATDVCPLIPIAEVSM
                     EECAILARNLAKRVGEELSIPVYCYENSAFKTERRNLANCRSGEYEGLAKKFEDPEWK
                     PDFGPDKFEGNVVHSGATAIGARDFLIAVNFNLNTTSTRRANAIAYDVREKGRKLREG
                     NSVSGKVVKDANGKDVWIPGTLKGCKAIGWYIEEYGIAQVSMNVTNITQTPLHVAFDE
                     VVAKAAARGIRVTGSEIVGLIPKKVLIDAGKYYLEKQQRSLGVAEEEIIKIAIKSMGL
                     DDLKPFNPKEKVIEYLIEDKNCKRLVDLTARGFTLETASESSAPGGGSVSAYMGALGA
                     ALGTMVANLSAHKPGWDERWKYFSDWAEKGQEIISELTFLVDEDTNAFNKIMNAFSMP
                     KGNDSEKKMREDAIQSATQYATEIPLKTMKVAYSAFDLLEAMIKDGNPNSVSDAGVGA
                     LAVRSAVYGAYMNVLINSVDLEDKSVAERLTGEAREVLDLSLERERELVDYVIQVLKS
                     RK"
     gene            complement(13716..14360)
                     /locus_tag="P5293_02995"
     CDS             complement(13716..14360)
                     /locus_tag="P5293_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR48901.1"
                     /translation="MAKFVTPLGNINVTIIGHASVMIDWNGRKIITDPYGEIADYTKL
                     PVMDLILITHDHYDHLDPVALRPITGDKTIIISTPAVAKILKGVRILRQGETYEFFGV
                     HITATYAYNINQRNEKGELFHPKGVGNGYILDFGGFKLYFAGDTELIPEMRDLGTIDV
                     AFLPKNLPFTMSDDMFIDAVNVIKPHNVYPYHYFHLDVNALKKRLPAEVKMFVK"
     gene            complement(14345..>14803)
                     /locus_tag="P5293_03000"
     CDS             complement(14345..>14803)
                     /locus_tag="P5293_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR48902.1"
                     /translation="KYNAISVDHLEFTGEEEFKTLHASETMATLLPGTTQFLEMDYAP
                     ARKMIDFGLSVAIATNYNPGSCPSGDMRYMMFLGALKLKMTPQELINAVTINGAYAMG
                     LEESHGTIAVGKIANFFITKPVPSIEYIPYAFTSPLVEKIYLNGKLWQNL"
BASE COUNT         4579 a         2880 c         2795 g         4549 t
ORIGIN      
        1 aagcctgccg ctagcgttca tcctgagcca ggatcaaact cttcattgta aaaaatgtat
       61 tccttagctt atcctgactt cgaattgttg acgaggttta aaaaacctct acctttgcta
      121 ctcattaatg ctatcaaaga actttctatt cagcgtttca atttcttcga aacgggctgc
      181 aaagatatgc gactttttta atctaacaaa attattcctc caaaatttat gaaaaatcaa
      241 tgctctcttt tctccgaaat taaattattt ttgcagcagt atgaatattt atctacaaat
      301 attttcaact ttttcgaaaa taggtgcttt tactattggt ggaggttacg ccatgctgcc
      361 tcttatacaa agagaagtag taacaaataa gaaatggctt agtgaagaag attttataga
      421 tgtactggcc atttcacaat ctacacctgg agtgtttgtc ataaatattt cagtatttat
      481 cggactccgc ataaaaggta taaaaggtag catcgttgct gcccttggta gtgcgttgcc
      541 atcatttgtg ataattttac ttattgcaat gttctttaat tcatttaagg acaatgagat
      601 agtagaaaaa atttttaaag gcatacgtcc tgcagttgtc gctcttatag ctgtacctct
      661 gattggaatg tcaaaaaaca tgaatcttaa cagatacact gcgtttatgc ctgttataac
      721 agttatgctg atagttttct ttcagatatc tcctatatat atattaattg cgggagcaat
      781 attcggaatt ttctatcctt atctttttaa gaaatgattt atttacagct atttataact
      841 ttttttaaaa tcggcctttt tacctttggt ggtgggtatg ccatgatatc gctgattcag
      901 aatgaggtag ttgtaaatca tcactgggtg gatgcagcct cttttaccga tatgatagca
      961 atatcacaga tgactcctgg gccaataggc ataaacagtg cgacatatgt aggatactct
     1021 gttaccggca atgcttttgg atctgcaatt gcaaccatcg cggtatctct tccgtctttt
     1081 attattattc tcacaattgc acatttttat gtccaattca aaaaaaatgt cttgtttgaa
     1141 tctgctatga atggtattaa accggtagtt ctcggattaa tcggagcagc tgcgactctt
     1201 ttggtcacaa aagaaacttt tatagactgg aaaagttgga taatttttgg tattgcattt
     1261 gctttgaccc agtggggcaa gttcaatccg atttatgtga tactattggc gggtcttgca
     1321 ggttatttaa tttactaaaa tatacttaaa ataagtaaga aatgaaaaag ttattaatta
     1381 caattattgt cctaacctcg acatttttca cgtcattggg acaggaacct aaatatactc
     1441 ctctttacta tcacagaagc tctctatttg aatcgcttcc aattaataaa tctgatatta
     1501 tcttccttgg taatagtatt attcattatt gtgaatgggc tgaattattc gataaccccc
     1561 atgtaaagaa cagaggcata agtggtgata tagttcaagg tgtttatgac aggctcgatc
     1621 caataattaa ggggcagcca aaaaagattt ttatcatgat tggcgtcaat aatttgtcaa
     1681 gggattacac tgtagattct acatatatgg gacttgtcaa aattgtagac aagctctcaa
     1741 aagagtgtcc ttataccaag ctctatatac aaagcgcatt gcctgttaac agcactttca
     1801 gcaattactc taaacacaga acaagaggtg aggagataaa aaagtataac actatggttg
     1861 aaaagatgtg tcaggagaag ggtattactt ttatcaattt ataccctctt ttcaagtgta
     1921 aggatagtga cgagcttgat cctgagttta ctagggatgg gcttcatatt aatggtgatg
     1981 gttatttggt ttggagggat gctattaaaa aatacgttaa atagtatgca aaaatatgag
     2041 gttatctaaa aagtaggcag cttctatttt tatatgtggt agtctattac gcatataaat
     2101 cggcgctttc gccacgggaa tctcaactaa cgtttaaaaa aagcgttgat atagttgaga
     2161 ttcccgtggc gaaacttttc taatatgttt ttattgcccc actgctcaag caagcttggc
     2221 agaatgcagg gattgcctcg cttaagcttg gcagtagggc aataaaaaac cagcgcattt
     2281 ctgcgccggc tttttctttg ggtggaagat gggattcgaa cccacgaccc ctagtgccac
     2341 aaactagtgc tctaaccagc tgagctacaa ccaccatata gcccataatt catacggggt
     2401 tgcaaagtta gctaaaaaaa tcaaataaag tattaacagt cgaaaaatag tgccagtttc
     2461 agatcttaag caaaatgtat caaatcgaca caatagatta ttcaaatatt tcttactttt
     2521 gccaaattaa attgtttata taatattttt ttgacatatg gctcgcgaaa ttaaattctc
     2581 tctgttatac agagatatgt ggcaatcatc aggcaaatat gttccaatgg tgcaccagct
     2641 aaaacagatt gctcctgtca tcatcgaaat gggttgcttc gaccgggtcg agacaaatgg
     2701 tggtgctttt gaacaagtta atcttctctt tggtgaaaat cccaataaag ctgtaagaga
     2761 gtggactgct ccatttaata aagccggcat tcagactcat atgctcgaac gcggcctcaa
     2821 cgcacttcgc atgaatcctg ttcctgctga tgttcgtgaa cttatgtaca aagtcaaggc
     2881 taaacaagga actaatatat cacgttcttt ctgcggacta aatgatccga gaaacttgga
     2941 actgtctgta aaatatgcaa aaagtggagg gatgatttct caggttgctt taagtatcac
     3001 tcattctcct gttcatactg ttgaatatta tatggatttg gttgacaaag tcgtagagtt
     3061 cggtgctgat gaaatttgct tgaaagatat ggcaggcatc ggtcgtccgg cgtttctagg
     3121 aagattgacc aagtctatta aggataaata tccaaaaatg atagtccaat atcatggtca
     3181 tagcggcccc ggtttttctg tcgcctcaat gctcgaagtt gcacgcgcag gagcagatta
     3241 tattgacgta gctatggagc ctctgtcctg gggaatggtc catcctgatg tcataacaat
     3301 tcaggcaatg cttaaagatg ccggattcct ggtcaaagat atcaacatga atgcctatat
     3361 ggaggcaagg agtcttaccc agtcattcat tgacgattat ctgggatatt ttattgatcc
     3421 aggtaataaa gtcagcacat ctttactggt gggttgcgga cttccgggtg gaatgatggg
     3481 atctatgatg gcagacctta aagggttcca aggtgcaatt aatgtttcac ttagaaatca
     3541 agacaaaaag gaacttaaac tcgacgagct tgttgttatg cttttccagg aggtcgaata
     3601 tgtttggccg cgtttgggtt accctcctct cgtgactccg ttcagccagt atgtcaagaa
     3661 taccgcactg atgaacctta tgcatattct taaaggtgaa ccaagatggt caacaataga
     3721 caaggataca tggaacatga ttcttggcaa aaccggcaaa ttgcctggtg aacttgctcc
     3781 agagatcaag caacttgcaa aagaaagagg aatggaattt tatgatggca atccacagga
     3841 tgcatttcca aacgaacttg atcgcttcag aaaagagatg aaggaaaata attgggattt
     3901 tggtgaagat gatgaagagt tatttgaatt tgctatgcac gaaaggcagt atagagatta
     3961 caagagcgga gttgcaaaac agcgtttcaa tgcagaactc gaagcagcca gggcaaaagc
     4021 taatgctcca atcgttgttg ccaggcctgt tgtagaattg cctaaggtgg atgtcgagaa
     4081 aattcttgct acttatccta atgcaaaacc tgttcaatca actgtcaagg gtcaacttat
     4141 atggcaatat gacatgattg acaagtcgtc tgctcctaat ataggtgaag tggttaaagc
     4201 cggcaagcca atgtgctttg ttcaagcctt ttacggattg gaagcaatta aaccggcctt
     4261 tgacggcaaa atagttgcaa tatgcgccaa acatggtgaa actgtacaaa aaggagaaat
     4321 aatcgctttt atagaataat taatattact tacccaattt tttaaaaagg aaaaatttta
     4381 aaatttttcc tttttttaat gcaacgaaaa tgcattaaaa tgcatctata aaatgattgg
     4441 caaataaagt aactatatgc tgaatattat gctttagagc ccatcagagg tgaggtacgt
     4501 ggattgaaga catataaggt gcttgtgatc tgtagtttgg gtagcagcaa acaaaggtga
     4561 acaaaaatct aaaatatgtg gaatattcaa ctagcctgca tcgacgaagg gaagttcctt
     4621 tttctaattc taacagtata atattcgaca aattgcagtg acctaggagg gattcaaacc
     4681 cccgaccttc agaaccggaa tctgacgttc tattcaactg aactactagg ccttgtattg
     4741 agcagcaaag gtactaattt taatattcat taaaaaatgt atctttgctg cttaataatt
     4801 tatgcatcat gaaagcaatc gttttccccg gacaaggggc tcaattcagt ggcatgggaa
     4861 aagagcttta taattcatat cccgaagcca gagaactttt tgaaagagct aatgagattc
     4921 ttggattcag aataagtgag ataatgtttg aaggaagtga tgaagcactc aaacagacaa
     4981 aggttacgca acctgcagtt tttattcact ctgtaatact atcaatatgt cataaggatt
     5041 tgatgcctca catggttgct ggtcactctt taggagaatt ttctgcttta gttgctgcta
     5101 aagccatttc ttttgaagac ggtttgaaac tagttgctgc aagagcatcc gccatgcaaa
     5161 aagcttgtga actggaaccc tctaccatgg ctgcaattct tggcctcgat gatgaaattg
     5221 ttgaacaagt atgctcttct atccagggaa ttgttgtgcc tgcaaattat aacaccaaag
     5281 gtcagctcgt aatatccggt tcagtagatg cagtcgccaa agcgtgcgaa gcccttaagg
     5341 ctgcgggagc aaagagagca ctgcccttgg cggttggtgg agcttttcac tcacctctta
     5401 tggaaccagc aagaaaagaa cttgcagaag ccatagctgt gacatatttt aacaaacctg
     5461 catgtccaat ttatcagaac gttgatgcca aacctcatac tagccccgaa gaaatcaagg
     5521 aaaatctgat tagccagttg acttctcctg taagatggag gcaaagcatc gaaaacatgt
     5581 gggcagatgg tgcaagagaa tttattgaac ttggccctgg aaatgtcctc cagaatatga
     5641 ttaaaaagat actccctgag agtacaacag gcggttatca atagcaaatt aacgtattac
     5701 aaatttatag agtaaggaca catcttctct tttagaaaga tcgggattgt gttctgaaat
     5761 ataattttca atttcaggat atttttttgg ataaaatttc ttaagtgttg gtttgttaac
     5821 agggaagagt ttcccgtctt taaccaaaaa aacatgctct ttgtagttaa atggaactga
     5881 accagaccat agacctttct caatctgata ataaaaatta tcttcagtcg aaacaatatt
     5941 gttcatggag gctatatcat ttgtaattcc gaaaaccccc tcctggactt tccttccaaa
     6001 ggtgatttgc cttacaacgc ccagacttgt ctctccgtca gtatctaata tctggacata
     6061 attattttta attttcaaaa aaaggtaacc gccggcacta agagatacaa ccatatcttc
     6121 agccgcaaca gtcactgtat cgcctttctc atcaatcatc cttaaagttt gggaacaatt
     6181 gtcaatattg aaaataccgg aataacttct acgatcttta agcataatta taccctttga
     6241 aaatgaaggc atcgcatacc tgtttttttt aaacaattca ctgtcggcaa ttttcccgtt
     6301 ttgaactttc agaatgccgt cctgggcaaa accgattcta acagacaaac tgagtataat
     6361 tgctattaga caggtgtttg caacaattct tttaatattc cattcatctt tcatatagga
     6421 tttatcttta tggtttatag tggcatgaat ttagcaaaat aaactttata ttataaaata
     6481 aatgactaat tttgtaaagt agataaaatg ctaaaaccta taccattcat aaaggagttt
     6541 gagacagaaa cagagttctt tccggcaaag tccttcattg ttgactttgc cgcggtgata
     6601 tttttaaaga tataaatatc ggtcagtaat acaattgtgt taccggcctt tttaatttta
     6661 tatattatcc tgttctaaaa cacaataaca atcaatataa atctataata aaaaagccat
     6721 ggcaaaggaa aaaaaattta ttacctgtga tggtaactac gccgcagcac atgttgccta
     6781 tcttttcagc gaacttgcag caatttaccc tattactcca tcttctccga tggcagagta
     6841 tgtcgacgag tggagcgcct tcggaaagac aaatcttttt ggagagaaag tgaaagttgt
     6901 tgagatgcaa agtgaggggg gtgcagcggg tgcgttacac ggagcactcc aggcaggtgt
     6961 actggctaca acatttacag cttctcaagg tcttttattg atgattccga acatgtataa
     7021 aattgctgca gaattgcttc caacagtttt tcatgtatct gcaagaacat tggcgacaca
     7081 tgctctgtca atttttggag atcattctga tgtgatggct acccgacaga caggttttgc
     7141 aatgatagca tcaagcagtg tccaggaaat catggatctt ggcgctgtag cacatctctc
     7201 tactctaaag tctaaaatac cttttgtaca tttctttgac gggttcagaa cttcacatga
     7261 aatccagaaa attgaaatgc tcgaccaaga ggcattaaag gcaatgataa gtgaaaaatc
     7321 tctgatagct tttagaaaca aagctctcaa tcctgttagg cctgtaacaa gaggtaccgc
     7381 acaaaatcct gatgttttct tccaggcaag agaagcttgc aacccgtttt atgatgctgt
     7441 ccccgacata gttgccagat atatgggcga gataagcgag ttgaccggaa gacattattt
     7501 gccttttgac tattatggag atcctgaagc tgaaaatatt ataatagcaa tggggtcagt
     7561 ttgtgaaacc attaaagaat cagtcgatta tctgactgaa aaaggagaaa aagttggcct
     7621 tctcacagtt cgtctttaca ggccattctc tgctaaatat ttcttccgtg ctttacctaa
     7681 gagcgttaaa aagatagccg tactggacag aactaaagaa ccgggttcga caggagaacc
     7741 tctctacctc gacatcaaat ctgtgattgc agaccaaggt gtcaaaatgc ctttgattat
     7801 tggcggtcgt tacggactct cttctaaaga tacaagtcct gctcaaatac tttctgtttt
     7861 tgaaaatctt aaactcaaag aacctaaaaa cggctttact atcggtatcg tagatgatat
     7921 cagcttcaaa tctttacctg tcaaggagga aatcagtctt gccaaatcag gtgtaaagga
     7981 atgtaaattc tatggccttg gttcagatgg tacagtagga gctaataaaa atacaataaa
     8041 aatcattggt gaaacaactc caaagtatgt tcaggcatat tttgactatg actcaaaaaa
     8101 atccggaggg tatacttgtt cacacttaag atttggcgat tctccgattc aatcgcctta
     8161 tcttgtttca actcctgatt ttgttgcctg tcatgttact tcttatcttc acaaatatga
     8221 tattctcaag ggcatcaaga aaggaggcac tcttctcctg aacaccttgt ggtctgtaga
     8281 ggaaaccaag gaaaagcttc cagattatat taaagccaca atagctaaga aagatctgaa
     8341 actttacatt atcaatgcta caaatattgc cgaagaaata ggtttgggca atagaacaaa
     8401 tacaattctt cagtcagcat tcttcaagat tacaaatata atcccttacg atcaagcagt
     8461 taaagagatg aaaaaggctg tcgaaaaatc atatggaaga aagggtgagg caattttgaa
     8521 aatgaactac gacgctattg acagaggtgc agaatacgtt caggtagata tcccttgtga
     8581 gtggaagaat ctgaccgcca aattccgtcc ttctaactat gacaggcttg cacctgactg
     8641 ggtaagaaat gttgcagacc ctgtaaatgc acaaaatggc aatgatcttc ctgtaagtgc
     8701 attcctcaat ttggaagatg gtactattcc tgccggaact gctgcatatg aaaaaagagg
     8761 tattgctgtc aatgtgccag aatggcaaag tgaaaactgt atacaatgta accaatgtgc
     8821 ctatgtttgt cctcatgctg caatacggcc atttttgatg agtgaggagg aacaggctaa
     8881 tgctccaaaa ggtattaaga aacttaccgg taatggtcca ttcaaagcat acagcttcac
     8941 aatacaggtg tctgtgatgg attgtacagg ctgcggaaac tgcgttgata tatgcccagc
     9001 aaaaaatcaa gccataataa tgaagcctct tgaaacacag cttcatgaac agagcaactt
     9061 tgactacctt aattcggttg ttggatataa ggacacaata cagccaaaga cacagaatgt
     9121 caagaatctc atgttttctc aaccattatt cgaattttcc ggagcttgtg ccggttgtgg
     9181 agaaactcct tatctgaagg ctatatccca gctttttggt gacaggatga tggttgcaaa
     9241 tgcaacaggt tgctcatcaa tatacagcgg ctctttccct tcttcaccat ataccacaaa
     9301 caaggatgga tatggtccat cctgggcaaa ttctttgttt gaagataatg ctgaatttgg
     9361 tttagggatg agacttggtt cagaaagagt tcgtgacagg attgcaaaac ttatggcaga
     9421 tggactcgaa tgcgataaat gttctgcaga aacaaaaatg ttatttggcg agtggctctc
     9481 caaacgcaat gaccctgcag ctacaagaga gattgccgat aagcttgtgc ctttaatcac
     9541 atcttgcaat tgtgacattt gcaaagaact gaccactttg aaagattttc tggttgttcg
     9601 ctctcaatgg atagttggag gtgacggatg ggcatatgat attggatatg gtggactgga
     9661 ccatgttctt gcatctggtg aaaacgttaa tgttatagtg cttgatactg aagtatattc
     9721 taatacaggt gggcaatcct caaagtctac ccctgcaggt gccgttgcaa aattcgctac
     9781 ctccgggaag aagattcgta aaaaagacct tggaatgatg gctataagct atggatacgt
     9841 gtatgtcgca caggttgcaa tgggtgctaa caacaaccag ctccttaagg ccctcaagga
     9901 ggcagaagaa tataatgggc cttctctcat cattgcctac tcaccatgta ttaaccacgg
     9961 actgaaaaat gggatgggtg cgtcccaaag agaagaaaag ttagctgttg aatgtggtta
    10021 ctggcatctt tacagatata atcctgctct cgaagcagaa ggcaaaaatc ctttcactct
    10081 cgacagtaaa gagcctgact ggagtaaatt tcaggatttt atcaaaggag aagttcgcta
    10141 cagttcactt gttaaaacat tcccaaagga agccgaagag ctgtttaaac ttaccgaaga
    10201 gtatgctaaa atgagatata attcatacaa aagattggcc agcatttagt agtctctaaa
    10261 ctgattgaaa tgaggttgtc tacttttaga cagcctcatt ttttatgtgc aattgcttta
    10321 gaacagtatt atctttttct cctcttcagg gatatcacca ctctcaagga taacatcttt
    10381 tattttagac tttcttgccc tcacacttga ataaggaatg gagagaataa ttgaaatatc
    10441 tttatttgtg aggccactct ttaccagaac cagcataaga atgtcaattg gagaacaaat
    10501 cgagcttaag taacgggtat atggataagc agatataaat ctattactat ttacaatctg
    10561 tgaatactga tccttcatct cctcctttgt ccttttaaca aatttgtgaa gattgtctga
    10621 aatggtctga ttgtatcgcc tcgatctttc agcttcgttg aaaacatctt ctataagatc
    10681 aggcattgac aaggcattgg ctttgctgat ctcactcaac agccattctt tttcttcaat
    10741 accagcaaat gagatatttg atttccttaa gtagttattt tttaatacgt tataagtcaa
    10801 aaaaaagaaa agaactacaa cgaccaatat cagatataat tgatgtttct ctgctttgtc
    10861 aagctgagta ttaagctttg caacttcatt tttgttatct atttcaagaa gtttttcacg
    10921 tgttcgggtt gtagaatatc ttaagagatt ttcgtatgcc tccttgtaat agttacatgc
    10981 agactgaaaa tcaccatgct tctgaagaaa caaagcatag cgcgctgggt atgtataact
    11041 tttatctgca acagtatcat ttgattgttt gagcgtttca agaaaatagg tctcagcact
    11101 gtctcttata cctttattgt cataataaat agcgagccta taatttaaac tttgataatc
    11161 aacatcttta agattatatt tcttagcaag atttagcatt cttttgagat aagcagtagc
    11221 tatctccgac tctctcatac ttacataaaa gatatacatg gcattacaca attcatattc
    11281 catagcaggt gatgcatttt gagcaaattg aattgatgct atgctctgaa aggctttggg
    11341 aatattaccc attaaaagat atagtcttaa tagttttgtc ttaagtctta caattcccac
    11401 tgaatcattt aactcggaag cagcctttat ggctttgttt aaatagactt cagcaagggg
    11461 aaaagctttt tgagctctca aaattttact tttgtactca taacactttg aaagaatata
    11521 tggatcttca agactatatg cttgataaac agaatcggcc ttaatcagat aatcataagc
    11581 agtagaatcc tttgaacctc ccattgaaat tagaatacca ttatacagca atgctctgca
    11641 atagtttctt ccatcttttc tatgcgactg ataccatgaa agagatttac cgatattctc
    11701 aatttcattt tctctgaatc ccattgcagc cctctcttta gccatacttc tcagtaaata
    11761 ataataggct tcgttctcac cctccaaaga aaatttatcc agttggttaa ggctatctag
    11821 tagagcttca gaattttgaa taattaaatg ctcccaagat tgcatgcgag aatataactt
    11881 ggcattttca ttcttaattc catcacatga aattataaaa aacagtaata gcaataagag
    11941 tcttcttcgc atattaataa cctaaaactg caataaagat actactcaaa attcaaaaaa
    12001 acactattta cgagatttca aaacttgaat cacataatca acaagttctc tctctctctc
    12061 aagtgaaaga tccaacacct ccctagcctc gcctgtaagt cgttctgcta cagacttatc
    12121 ctcgagatcg accgagttaa tcaacacatt catatatgca ccgtaaacag cagatctgac
    12181 agccaatgct cccactcctg catctgaaac agaatttggg ttgccatctt ttatcatggc
    12241 ttcaagaaga tcaaaagctg aatatgccac tttcattgtt tttagaggaa tctccgtagc
    12301 atattgtgtg gcagattgta tagcatcttc tcgcatcttt ttttctgaat catttccttt
    12361 aggcatggaa aatgcgttca taatcttatt gaatgcatta gtatcttcgt ctactaaaaa
    12421 agtcaattcg gatataattt cctgaccttt ttctgcccag tctgaaaagt atttccacct
    12481 ttcatcccat cccggtttat gagccgaaag atttgcgacc atagtgccga gtgcagcgcc
    12541 aagagcaccc atataggccg agacagatcc tcctccgggc gcggaagatt ccgaagctgt
    12601 ctccaaagta aatcctcttg ctgtaagatc aacgagcctt ttacagtttt tgtcttcaat
    12661 cagatactca ataacctttt ctttaggatt gaaaggtttg agatcatcaa gccccattga
    12721 cttaatggca atttttatta tttcctcttc agcaactccc agggaacgct gttgtttttc
    12781 caaataatat tttccggcat caataagaac tttcttagga atcaacccga caatttccga
    12841 ccctgttact cttatacctc ttgcagcagc tttagcaact acctcatcga aagccacatg
    12901 taacggtgtc tgggtgatat ttgtaacatt cattgaaact tgtgctattc cgtattcctc
    12961 tatataccag ccaatcgcct tacatccttt gagagtgccg ggaatccaaa catctttgcc
    13021 gttcgcatct ttaacgactt tccccgaaac agaatttccc tccctcaatt ttcgtccttt
    13081 ttctcttaca tcatatgcaa ttgcatttgc cctgcgggta gatgttgtat ttagattgaa
    13141 attaacagca ataagaaaat ctctggcacc tatcgccgtt gcacctgaat gaacaacgtt
    13201 tccttcaaat ttgtccggcc caaaatccgg tttccattca gggtcttcga acttctttgc
    13261 cagaccttcg tattcaccag atctgcaatt ggccagattc ctgcgctcag tcttaaatgc
    13321 cgaattttca taacagtata caggtattga aagttcttcc ccaactcttt tagccagatt
    13381 tcttgcaagt atggcacact cctccataga aacctcagca attggaatta gaggacaaac
    13441 atcagtagct cccattcttg gatgctctcc gtgatgacga ctcatatcaa tcacctcgga
    13501 agcttttttt atagccctga atgcagcctc gacaaccgct tcaggtgctc ctacaaatgt
    13561 cactaccgtc ctgttagttg cagcacctgg atcaacattc aacagcttaa caccttcaac
    13621 actttctatt tcatctgtaa tctgttttat tacagacatg tttctccctt cgctgaaatt
    13681 ggggacacat tctattagct ttttaatcat aacatctatt ttacaaacat tttcacctct
    13741 gccggcaatc ttttcttcaa cgcattaaca tccagatgaa agtagtgata aggataaaca
    13801 ttatgtggtt ttataacatt taccgcatct ataaacatgt catcactcat tgtaaaaggc
    13861 agattcttgg gaagaaaagc aacgtcaatt gtaccaaggt ctctcatctc agggattaac
    13921 tctgtgtctc ctgcaaaata aagtttgaaa cctccaaaat caagtatgta accatttccc
    13981 actcctttag gatgaaacaa ctctcctttt tcattacgtt gattgatatt ataagcataa
    14041 gttgcagtaa tatgtacacc gaaaaattca tatgtctcac cctgcctcaa aattctgaca
    14101 cctttgagaa ttttagcaac tgctggtgta gatatgatta tagttttatc ccctgtaatc
    14161 ggcctaagag ccacagggtc caaatgatca tagtgatcat gcgttatcaa aataagatcc
    14221 attaccggca attttgtata gtcggctatc tctccataag ggtctgtaat aattttacga
    14281 ccattccagt caatcattac tgatgcatgt ccaataatgg tgacattaat atttccaagt
    14341 ggtgttacaa attttgccat aattttccgt ttaaatatat cttctccact aatggagatg
    14401 taaatgcata aggaatatat tcgatagaag gcacaggttt ggtaatgaaa aagtttgcaa
    14461 tcttaccaac ggctatcgtc ccatgtgatt cttccaaacc catggcatat gccccgttaa
    14521 tagtgacagc attaataagt tcctgtggag tcatctttaa tttaagtgcc ccaagaaaca
    14581 tcatgtacct catgtctcct gaagggcatg acccaggatt ataattggtt gctatggcaa
    14641 cagaaagtcc aaaatcaatc atttttctgg ccggggcata atccatttcc agaaactgag
    14701 tcgtgccagg caaaagtgtt gccatagtct ctgaagcatg tagagtttta aattcttctt
    14761 cacctgtaaa ttccaagtga tcgacagata tggcattata ttt
//
LOCUS       DAOTQL010000028        14716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3920_length_14716_cov_4.814065, whole genome shotgun sequence.
ACCESSION   DAOTQL010000028 DAOTQL010000000
VERSION     DAOTQL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_14716_cov_4.814065"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="P5293_03005"
     CDS             complement(<1..394)
                     /locus_tag="P5293_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR48903.1"
                     /translation="MRKAYNILTICSLLLTLNSCGNDWLNLEPSDRIETSRSISTLND
                     VQAAVVGIYNQLQNYEYYGARMTYYGDVTGDDMQSNGDTKRCASFYRYAYTAENAPRS
                     LWEQPYKVIRLANNILAVIDNIKVTDAQK"
     gene            complement(406..3594)
                     /locus_tag="P5293_03010"
     CDS             complement(406..3594)
                     /locus_tag="P5293_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR48904.1"
                     /translation="MIERDYKIRFFYDEKTIDVNSIKSISLKNITINELVNALFEGSA
                     SCKISENGLVTLVKSELNEKARENDKVTVTGLITDESKNPLVNVYVYIKGTNLGTYSD
                     QNGRFTLKNVPLHSVINFDYIGMKSKTLQVLQENERFIVTLEEDVSILEDVIITGYGM
                     LKKSAYAGSASVVKTENIKDVPTMDLSAMLQGSAPGIQVTGSSGQPGAVPTVRIRGIG
                     SFNASNSPLYVIDGVPVISGDINNSGSDAGTNIMATINTNDIENITVIKDAAAASLYG
                     SRAANGVILITTKTGKKGKAQFNFKTSYGFTDFAYRYRPVMFGDERRNFIYNSMIRRA
                     LYQDGKSETEAIAYAEANINSVAAKPWCGWVNWEDVLLRKGSQMDYEFSVSGGEEKIS
                     YFSSLSYSNQEGIQYQQGLERISGRLNVKYDANKWLQLGSNIVFSDMDQDVGYDGMEY
                     VSPIYSSKHKLTCSDAVYNPDGTYNENLQSNGARNPKAALDLNYDKQRVTRSFNTLFA
                     QVAFSEDLKLKTTLSYDYTVTKARSWTDTRTSSGAATNGSAYKSYYDYNQMVWSTNLN
                     YIKTFGNSHHIDALIAYEISDLKSDYLSGTQVNFINPEYNAIGNGTQPRSIGGYPRQN
                     RLVSYISKINYDWNGTYYAGVSYRLDGTSRLHPDKRWGDFWSISGAWRFSNEDFFKPL
                     KEWISDAKFRASYGVNGNLPSSYYAYMSLTDVDASYNEQPGIAEGNIANNNLLWETNY
                     TTNFGLDFSVKRILDFSLEYYIRKTKNLLMDMPTSLTSGFSSYTTNIGNVKNKGIELQ
                     INTVNIKNNNLTWTSTFNLGHNRNKILVLDGIQTEIISGTQIRRVGLPYYTYYMIEFA
                     GINPDNGKPQFYTNTKDENGKYVKDITENSSEAKPIANKSPFPDVTMGLTNTLKYKII
                     DFSFTLTSTFGGWSYDSAAQKSQTSGSGDGAINQIPLYYKNSWQKPGDITKYEAWIYG
                     NSFKMSSPANSRRLHSTDHVRIKNLTFGISAPKLITNKLGLNSARIFFSAYNLWTFAA
                     FREYDPEVPIDGSVAYNTPPLKTLTFGLDINF"
     gene            complement(3837..4403)
                     /locus_tag="P5293_03015"
     CDS             complement(3837..4403)
                     /locus_tag="P5293_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRR48905.1"
                     /translation="MKDLVSKIISGDEEAFEYIKNKWNLRLKIFARSYVRSDDVSEDL
                     IQDTYIKFWESRAVVNNEKMIGPYLFTILKNKCLDYLKHKIIEQKYNNVTSERYYLKA
                     NIYALEDRSINIITEEQIKKALLKAIDKLPQPRKDIFIMSRFKEMKNAEIANRMSLSV
                     KTIEYHITKAISFLRSKLEDYYNYIIFF"
     gene            4756..5007
                     /locus_tag="P5293_03020"
     CDS             4756..5007
                     /locus_tag="P5293_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48906.1"
                     /translation="MDNDLTILLETCDKVIAEDIQVLLEASQVYSVLVSDNPASSVLN
                     IYSGFNPIENIIIKVNKNDYLKAVEIIKDSAYKEFLANA"
     gene            complement(5158..5230)
                     /locus_tag="P5293_03025"
     tRNA            complement(5158..5230)
                     /locus_tag="P5293_03025"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5195..5197),aa:Lys,seq:ttt)
     gene            5373..6140
                     /locus_tag="P5293_03030"
     CDS             5373..6140
                     /locus_tag="P5293_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48907.1"
                     /translation="MEFLRDYSLAFTRINMIFIVQKCGSLHSNEQLSIRLIPICRQSK
                     SQDGFSADVDINIFEEEWINKKEIVINRLKANLGLQKTIYARKCVAREISVADAIQFL
                     NANHLLGYTRSNYRYGLFATEDSETVKSESLVAVATFSGPKIINREGTNVKSYEWVRY
                     ANYSDFRVVGGMGKLLDFFIQKEFPQEIMSYADRSWGNGNAYVKLGFKLLETTCNITY
                     YLDRKSLERVPKRLLSKIDKTELLVRHKSDFKFIRRF"
     gene            complement(6096..8615)
                     /locus_tag="P5293_03035"
     CDS             complement(6096..8615)
                     /locus_tag="P5293_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRR48908.1"
                     /translation="MDLHLSNELNEILSYSKEEAMRLGSYTIDNDHILLGILRHQDNS
                     AFEILIRSGVDYKELKQRIEEGIRAGEIVPFEQSDKLNLSKGADNSLKLMYLEAKSLN
                     EAQPGPIHLLLAILRSNDAYAIPILKEFNVTYDTIRSLLKGKENVIPETGMEAAGEIN
                     NPASSESGSKISKSSKLTSDTPVIDSFGFDMTAAALEGKLDPVVGREKEIERLAQILG
                     RRKKNNPVIIGEPGVGKSAIAEGLALRIAHRNVSRVLLNKRLVSLDIGSIVAGTKYRG
                     QFEERMKAILNELSKNPNIILFIDELHTLVGAGGASGSLDAANMMKPALARGEIQCIG
                     ATTLDEYREYIEKDGALERRFQKIMVEPTTYDETLNILRNIKERYEKHHNVIYTDDAL
                     VACIKLSQRYITDRCLPDKAIDAMDEAGSRVHLKHLQVPDSIVKIESEIEPIRKQKKL
                     AANNQQFEKAAELRMIEKQMMDELEAAQNSWNEEQTKNPPVVNSNDVAEVLSMITSVP
                     IHRLAEKEGDRLARMSEAIKRKIIGQDEAVEKVVRAIQRNRAGLKDPNKPIGTFLFLG
                     PTGVGKTQLAKTLTEFLFDSVDNLIRIDMSEYMEKFAVSRLIGAPPGYVGYEEGGQLS
                     EKVRRKPYSVVLLDEVEKAHPDIFNLLLQVLDEGRLTDSNGRRIDFRNTLLIMTSNIG
                     SREVKDFGAGLGFSNTTKRNVNDNTRQIIEKALKKTFSPEFLNRLDEYILFNPLSKED
                     ICKIIDIELDDLFKRIDNVGYKLSIDDKAKMFVADMGYDPQLGARPLKRAIQKYIEDP
                     LAEAIITGRFKLGDELHIELDTNLEDPSKSPDKLKVTLVSH"
     gene            8729..11230
                     /gene="gyrA"
                     /locus_tag="P5293_03040"
     CDS             8729..11230
                     /gene="gyrA"
                     /locus_tag="P5293_03040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRR48909.1"
                     /translation="MIEESDSNPRIIKINIEDEMKSAYIDYSMSVIVSRALPDVRDGL
                     KPVHRRVLYGMSELGLTSGSGYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSMR
                     YMLVDGQGNFGSIDGDSAAAMRYTEAKFRKIAEEVMADLDKNTVDMKPNFDESLLEPT
                     VMPSKAPLLLLNGASGIAVGMATNMPPHNLGEVVDAICAYIDNNEITTEELLKYLKGP
                     DFPTGGIIQGTQGIKDYFETGRGRIIVRAKTEIEVNDSGRETIVVTEIPYMVNKREMI
                     EKIAELVENKKIEGIAYINDESDRKGMRIVIKLKMGAVANVVLNMLFKYTQMQSSFSV
                     NNIALVDGRPRILSVKQIIKYYVRHRHDVILRRSRFELEQAEKRAHILEGLLKALDFI
                     DEVIAIIRGSKRVEDARNSLMDRFSFTEQQASAIVEMRLRQLTGLEREKLQSEYEELE
                     KQIAYLNQILSQVSLQMQIIKDELQELKTKYADPRRTMIELSEDEFNPEDFYADEDVI
                     ITISHLGYIKRTPLNEYRTQNRGGVGVKGGATRDEDFIENIYVANMHSTMLFFTKKGK
                     CYWLKVFDLPEGSKISKGRAIQNVLNIEPDDKACAFINVHNLSDKEYVNNNYIVLCTK
                     RGIVKKTSLEAYSRPRANGVNAITIKENDELLEATLTNGKNEILLASREGKCVRFNES
                     CVRAIGRTGAGVKGINISEDNEVIGMICVDTDTKGSGETKHVLVVSENGYGKRSLLDD
                     YRVVNRGGKGVKTLSITEKTGKLIAIKDVSDDNDLMIITKSGLTIRLEVSSIRVSGRA
                     TQGVKLINIKEGDSIAAVCRVNKSEEKPVETEELE"
     gene            11293..12609
                     /locus_tag="P5293_03045"
     CDS             11293..12609
                     /locus_tag="P5293_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR48910.1"
                     /translation="MKKILLALTLLFSLQLAAQTKEVADAIKNYEKALKESADAKKST
                     NPATWIKLADACAAVYDTPVKSIWNGASRNEIKLLLKDQRIISTEDRTVLDVKYTVDI
                     YDDKELYYNQDGTLACFVQTKNYITGNPIDDGVAALLKAAELDVKKAKAKDITDRLLA
                     MKNRYQTEAMTAYTLGDFKKASYNFEGCVNISSNPLINQPDTVMMYYTGLTALMAKEY
                     DRAVKFLQMSIENGYDAKGDIYSSLAEAYKSSGNVEKAKEVLQAGFTKYPTNQSILVM
                     LINSYLESNDDPNKVLTLISQAQKNEPNNASLYYAEGNVWKNLKNIDKAIECYKKSVE
                     IDKSYYFGSFAIGAAYYDRAVEIQAAATEEVDDNKYEELVKQLEENLMAAIEPFENCF
                     ASTSDKEVKLVVAEYLKNIYFRFREKGDDYKAKLEKYTNYLNENKK"
     gene            complement(12789..>14716)
                     /locus_tag="P5293_03050"
     CDS             complement(12789..>14716)
                     /locus_tag="P5293_03050"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR48911.1"
                     /translation="GCTGELISEKGLLITNHHCGFSYIQSHSTVEHDYLKDGFWAMNM
                     EEELPNDNLTVSFLVKMEDVTDAVLKSYLPSMSESERQKIVAENSARIIENATKGTTY
                     KGSIESLYYGNQYFLFIYETFYDVRLVGAPPSSIGKFGGDTDNWMWPRHTGDFSIFRI
                     YADKNNRPAKYSKNNVPYRPKKYFSISTTGINEGDFTLVYGFPGKTQEYLMSSAVKYI
                     SEVSNPHKIKLRTLRLDVQKEYMSQNQEIRIMYAAKNANVANSWKKWQGEMNGIKRMN
                     IVEKKTEYEKRFNEWAKNRDEYKNIVSSLDSLYKMLEPYSFANDYNNEAINSNEILKF
                     TGSLLTCFKGKDGNIKNEDKDSVKNIIELFFKDYHLPIDKKCFVLLIDEYRKNIPLKF
                     QPEYFISKMREYEGDIYKFADYVFDNSIFANQSQCMKILQDSVTISTILKDPAVEMYF
                     AFKQKFDDDIKPEYNRLNNQITLLYRKYMKGQMEFDKKKDFYPDANSTLRVAYGKVSG
                     YRPADGIYYKPYSTLEGIIEKDNPQIYDYNIPQTLRDIHKNKDYGKWNINGTVPVCFI
                     ANNHTSGGNSGSPVINGKGELIGINFDRVWEGTMSDIAFDPDMCRNICLDIRYVLFLI
                     DKYANAQWILKEISINN"
BASE COUNT         4583 a         2524 c         2670 g         4939 t
ORIGIN      
        1 ccttttgtgc atctgtaact ttaatgttat caattactgc aagtatatta tttgctagac
       61 gtataacctt atatggctgt tcccataaag atcgaggagc attttctgct gtataggcat
      121 atcgataaaa actggcacat ctttttgtgt ctccgttaga ctgcatgtca tcacccgtaa
      181 catctccata ataagtcatt cttgctccat aatactcata attttgaagt tggttgtata
      241 ttcctacaac tgctgcctga acgtcattaa gagttgaaat tgatctggag gtctcaattc
      301 tgtctgatgg ctccaagtta agccaatcat ttccacaaga gttcaatgtt aataataaag
      361 aacagattgt taaaatatta tatgcttttc tcatgttaat tgcaattaaa agttaatatc
      421 aaggccaaaa gttagagttt taagaggagg ggtattgtaa gctacggatc catctattgg
      481 aacttcgggg tcatattcac ggaatgctgc aaatgtccat aaattatatg ctgagaagaa
      541 aattctggca ctatttaatc caagtttatt tgttattaat ttaggtgcac ttattccaaa
      601 agtaagattc tttatcctta catggtctgt tgaatgcaat cttctactgt ttgcaggcga
      661 agacatttta aaggaatttc catatatcca tgcttcgtat ttggttatgt cacctggttt
      721 ttgccaacta tttttgtagt ataatggaat ttggtttatt gcaccatctc cggagccact
      781 tgtttgagac ttttgtgcag cactgtcata tgaccatcct ccaaatgtag atgttagtgt
      841 aaaactgaaa tctattattt tatatttcag ggtgttggta agtcccatag ttacatcagg
      901 gaaaggagat ttgtttgcaa taggtttggc ttcggaactg ttttcagtga tgtctttaac
      961 atatttaccg ttttcatcct ttgtattagt atagaattgt ggtttcccgt tatctggatt
     1021 tattcctgca aattcaatca tataataagt atagtatgga agtccgactc ttctaatttg
     1081 tgtaccgctt attatctcgg tttgtatgcc atcaagaacc aaaattttat ttctattatg
     1141 gcctaaatta aatgtggatg tccatgtgag gttattgttt tttatattga ctgtattaat
     1201 ttgcaattca atgcccttgt tttttacgtt acctatattg gtggtatacg acgaaaaacc
     1261 cgaagttaaa gatgttggca tgtccatgag aaggtttttc gtttttctta tatagtattc
     1321 aagagaaaaa tccagtattc gttttacaga aaaatcaagt ccaaaattgg ttgtataatt
     1381 agtttcccat agcagattat tgtttgcaat attaccttca gctattcccg gttgttcatt
     1441 ataggaagca tctacatccg ttaaactcat atatgcatag taagatgatg gtaaattgcc
     1501 attaactcca taagaagctc taaattttgc atcgctaatc cattccttta aaggtttaaa
     1561 aaaatcttca tttgagaatc tccaagcacc tgatattgac cagaaatctc cccacctttt
     1621 gtctggatgc aatctggatg taccatccag tcgatagctc actcctgcat aataagttcc
     1681 attccagtca taattgattt tagaaatata tgatacaagt ctgttttgtc ttggataacc
     1741 acctatagat cttggctgag tcccattacc tatcgcattg tattcaggat taataaaatt
     1801 aacttgagta cctgacaaat agtcactttt taaatctgaa atttcatagg caatcagtgc
     1861 gtcgatatga tgactattcc caaatgtttt tatatagttc agattagttg accataccat
     1921 ctgattgtaa tcatagtaag atttataagc gcttccattt gtagctgctc cactagaagt
     1981 tcttgtgtct gtccatgacc tggctttggt tacagtgtag tcatagctca aagttgtctt
     2041 aagtttcaag tcttcactga atgctacttg agcaaacaaa gtattgaaag accttgtaac
     2101 tctttgtttg tcataattaa gatcaagtgc agcctttgga tttctagctc catttgactg
     2161 taaattttca ttatatgttc catctggatt ataaactgca tctgaacatg tcagtttgtg
     2221 ttttgatgaa taaatgggtg aaacgtattc cattccatca taaccaacat cttggtccat
     2281 atcagaaaaa actatatttg agccgagctg taaccatttg tttgcatcgt actttacatt
     2341 cagtcttcca cttattcgtt caaggccttg ttgatattga atgccttcct gattagagta
     2401 agagagagaa gaaaaatatg aaattttttc ttctccacca gaaacggaaa attcataatc
     2461 catttgagag ccttttctta atagaacatc ttcccagtta acccatccac accatggttt
     2521 tgcagcaacc gagttaatat tggcctctgc atatgcaatt gcttctgttt ctgactttcc
     2581 atcttggtat aaagctcttc tgatcatcga attatatatg aaattacgtc tttcatctcc
     2641 gaacatcacc ggtctgtatc tataagcaaa atctgtaaac ccgtaactag ttttaaaatt
     2701 aaactgtgcc tttccctttt tcccggtttt tgtagttata agaattaccc cattggcggc
     2761 tcgtgaacca tagagtgagg ccgctgctgc atccttgatt actgtgatat tttcaatgtc
     2821 atttgtattg atggttgcca ttatgttagt tccggcatca cttccactat tattaatatc
     2881 gccggagatt acaggaacgc catcaattac ataaagtgga gaattagatg cattgaaaga
     2941 tcctattcct cttattctta ctgttggaac agcacctggt tggccagaac ttcctgtaac
     3001 ctgaattcct ggtgcacttc cttgcaacat ggcagaaagg tccatggtag gcacatcttt
     3061 aatattttct gttttgacta ctgaggccga tcccgcatat gctgattttt taagcatgcc
     3121 atatcctgta atgataacgt cttctaagat agaaacatcc tcttcaagag taactataaa
     3181 acgttcattt tcttggagta cttgtaatgt tttggatttc atgcctatgt agtcgaaatt
     3241 tattactgaa tgaagtggaa cattttttaa agtaaatctt ccattttgat cactataagt
     3301 gccaaggttt gtgcctttaa tgtatacgta gacatttacc aaaggatttt ttgactcgtc
     3361 cgtaataagt cctgtaacag taaccttgtc attttcgcgt gctttttcat taagttcgga
     3421 ttttacaagt gtaacaagtc cgttttcaga aattttacac gaagcagaac cttcaaacaa
     3481 tgcgttaaca agttcgttga tcgtgatgtt tttcaaggat atgctcttaa tagagtttac
     3541 atcaatcgtt ttttcgtcat aaaaaaatct gattttgtaa tctctttcaa tcatctccaa
     3601 aattttttta ataggagaat tttcagctga aacagaaatt tttagattgt ttaaatcctg
     3661 agcagacaag ttgcctgata aaaaaatcag gcaacataaa agcaaagaaa tttgctttaa
     3721 tgcattttta caaaagttta ttctcatgtt caaagttatt agttaagtgg ttcttcgttt
     3781 gtcgttatta aactttcttt ttgtataacg actataatag aacaaaccct aatgaattag
     3841 aaaaaaatta tataattata ataatcttct aatttacttc tcaaaaaaga aatggcttta
     3901 gtgatgtgat attcaatggt tttaactgag agtgacattc tattggcgat ttcagcattt
     3961 ttcatttcct tgaacctgct cataataaaa atatcttttc ttggttgagg aagtttatct
     4021 atagctttca acagtgcttt tttaatctgc tcttctgtaa ttatatttat tgatctgtct
     4081 tcgagagcat aaatattagc tttcaaataa tatctctcgg aagttacgtt gttatatttt
     4141 tgttcaatta tcttatgttt taaataatcg aggcatttat ttttgagaat ggtaaagagg
     4201 tatgggccta tcattttctc attattcaca accgcacggg attcccagaa ttttatatat
     4261 gtatcttgaa ttaggtcttc agacacatcg tctgaacgga catatgagcg ggcgaaaatc
     4321 tttaaccgca aattccattt atttttaatg tattcgaaag cctcctcatc tcctgaaata
     4381 attttagaga caagatcttt catgataaat tttattttaa agttgattaa aaaattaaaa
     4441 ttaattcttt tttatcaaga tacaatttcg atttataaga aaaaccgaaa taaatggtct
     4501 gtgccataaa ggaactggag gaagataatc gcaagttgaa acagatgtat gccaatctgg
     4561 ctctggatta gaatacaagg aaatgaaaag ggttggataa aatatatatg attcactttt
     4621 tggggctgtc tgccccagtt gttcaagtaa actccgaaaa gtttttcata tgttaaagaa
     4681 taaattacta atttaacaat ctaaatcaag accgtccgaa aaagggaagt tgacaattta
     4741 aaaaacatat atacaatgga caacgattta acaattttgc tagagacatg tgataaggta
     4801 atagctgaag atattcaggt tttattagaa gcctctcagg tgtattcggt actggtctca
     4861 gataatccag catcttctgt attaaatatc tattcgggat ttaatccgat agagaacatt
     4921 attattaaag taaataaaaa tgattatctg aaggctgtcg agataataaa agatagcgca
     4981 tataaggaat ttttagcaaa cgcataacac caaggatagt tcagtcaccg aagtgtgtcc
     5041 aattatgacc attttttgac tattattcag gacaaacaaa ttgaaaataa aaaacctaca
     5101 atcatttgat attgtaggtt ttgtctagtt attgtccatt atttcggaca ttttttgtga
     5161 tccagctggg attcgaaccc aggaccccaa cattaaaagt gttgtgctct acctgctgag
     5221 ctactgaatc tttgattggg agcgcaaaga tattgcttat tggttttatt tccaaatttt
     5281 actttccttt attatattaa ttaggctaaa tttgcaaata ggcataacga tatttattaa
     5341 taatagtgga caaagacttt ttatatgaat ttttggaatt tttaagagac tactctttgg
     5401 cttttacaag aattaatatg attttcatcg ttcagaaatg tggaagtctc cattcaaatg
     5461 agcagttatc catccgactt attccgatat gtcgccaatc aaagtctcag gatggcttta
     5521 gtgcagatgt tgatataaac atttttgaag aggagtggat taacaaaaaa gagattgtta
     5581 taaaccgttt gaaagccaat cttgggttgc aaaaaacaat ttatgccaga aaatgtgtgg
     5641 caagagaaat atctgttgcc gatgccattc aatttttaaa tgccaatcat ttgttaggct
     5701 atactagaag taattaccga tatggcctct ttgcaactga agattcagag acagttaaaa
     5761 gtgagtcgct ggttgcagta gcaacatttt cggggccaaa aattatcaat agagaaggta
     5821 ctaatgtcaa gtcttatgag tgggtgaggt atgcaaatta ttcggacttc agggttgtag
     5881 gaggaatggg gaagctgctt gattttttta ttcaaaaaga gtttccccaa gaaataatgt
     5941 catatgcaga cagaagctgg ggtaatggca acgcttatgt taaactaggg tttaaattat
     6001 tggaaacgac ctgtaatata acttattatt tggacagaaa atctcttgag agagttccca
     6061 agagattact gtctaaaatt gataagactg aacttttagt gagacacaag agtgacttta
     6121 agtttatcag gagattttga gggatcctca agattagtgt cgagttctat atgtaattca
     6181 tctccaagct tgaatcttcc tgttattata gcttccgcaa gtgggtcctc aatatatttt
     6241 tgaatggctc gttttaatgg tcttgctcct aactgaggat catatcccat atcggccaca
     6301 aacattttag ccttgtcatc aatagataat ttgtatccta cattatcaat tcttttgaaa
     6361 agatcatcta gctctatatc aatgatttta caaatgtctt cttttgagag tggattaaat
     6421 agtatatatt catccagtct gttcaaaaat tctggagaaa atgtcttttt taatgctttt
     6481 tcgataattt gcctggtatt atcattaacg tttcgtttag tggtattgga aaatccaagt
     6541 cctgccccaa aatctttaac ctctctgctc cctatgttag aagtcataat gagaagagta
     6601 tttctgaaat caattcgtct gccgttactg tcggtcagtc ttccctcatc aagaacctgc
     6661 aacaaaaggt tgaaaatatc aggatgagcc ttttctactt catcaagtaa tacaactgaa
     6721 taaggtttcc ttctgacttt ttcgcttaat tggccaccct cttcatatcc gacataaccc
     6781 ggaggcgctc ctatcaatct actgacagca aacttttcca tatattcact catgtcgatt
     6841 cttatcagat tgtcaacaga atcaaacaga aattctgtaa gggtttttgc cagctgagtt
     6901 tttccaacac ctgtagggcc aagaaataga aaagttccaa taggcttgtt tggatctttc
     6961 aaaccagccc gatttctttg gatggctctt actacctttt cgacagcttc atcctgacca
     7021 attatttttc ttttaattgc ttcactcatt ctggcaagtc tgtctccctc tttttctgcc
     7081 agtctgtgga tagggactga tgttatcatt gaaagtactt ctgccacatc gtttgaatta
     7141 acaacaggag gatttttagt ctgttcttca ttccagctgt tctgagcggc ctcaagttca
     7201 tccatcattt gtttttcaat cattcttaac tctgctgctt tctcaaactg ctgattgttt
     7261 gcagcaagtt ttttttgttt gcggattggt tctatttcac tttctatttt gacaatcgag
     7321 tcaggaactt gtaaatgttt caagtgaacc cttgatccag cttcgtccat cgcatcaata
     7381 gccttgtcag gaaggcacct gtcagtaata tacctttgtg aaagcttaat acaagcaaca
     7441 agggcatcat cagtatagat tacattatga tgtttctcgt atctctcctt aatatttctc
     7501 agaatattga gagtttcgtc ataggtggtt ggttcaacca ttattttctg aaaacgtctc
     7561 tctaatgcgc catctttttc gatatattca cgatattcat caagtgtggt agcaccgata
     7621 cattgaattt ctcctcttgc aagagcaggt ttcatcatat ttgcggcatc aagtgaaccg
     7681 gaggctcctc cagcacctac aagagtatgg agttcgtcaa taaacaagat aatattggga
     7741 tttttggata actcattaag aattgccttc attctctctt caaattgtcc tctgtatttt
     7801 gttccggcaa ctatagagcc tatatcgagt gatacaagtc ttttgttaag taacactctc
     7861 gagacatttc tatgagcaat tctgagtgca agaccttcag ctatagcaga tttgcctact
     7921 ccaggttctc caattataac tggattattt ttttttctac gtccaagtat ttgtgccagt
     7981 ctttcaatct ctttttctct tccaactaca ggatcaagct ttccttcaag tgcagcagct
     8041 gtcatgtcaa acccaaaact atctattaca ggagtatcac ttgttaactt tgaacttttt
     8101 gaaatttttg atccgctttc agatgaagca gggttgttta tttctccggc tgcttccatg
     8161 cctgtttctg ggataacgtt ttcctttccc ttgagtaaag atcttatagt gtcatatgtg
     8221 acattgaatt ctttaagtat tggtatagca tatgcatcat tactccgtag aattgccagg
     8281 agaagatgaa tagggcctgg ctgtgcctcg ttaagtgatt ttgcttcaag gtacattagt
     8341 ttaagagaat tatcagcgcc ttttgaaaga ttaagtttgt cagattgttc aaatgggaca
     8401 atttctcctg ctctaatacc ttcttctatt cgttgtttta actctttgta atctactcct
     8461 gatctgatga gtatttcaaa tgctgaatta tcctggtgac ggaggatacc taaaagtata
     8521 tggtcgttgt caatagtata gctgccaagt ctcattgctt cctctttgct atacgaaaga
     8581 atttcattaa gttcgttcga aagatgtaag tccattacta taaaaattta ttgcaaatct
     8641 aattaaagac aatcagcaat acaatttgtt cagatgatat tttattagta ctttagcaga
     8701 ctttttttaa gctgaaagag aattaaaaat gattgaagaa tctgatagca atccaagaat
     8761 tatcaagatt aatattgagg atgagatgaa gagcgcttac atagattatt ctatgtcagt
     8821 gatagtgtca agagctttac cggatgtgag agatggtctc aaaccggtgc acaggagagt
     8881 tttatatggt atgtcggaac ttggacttac ttcaggtagt ggatataaga aatctgcacg
     8941 tattgtagga gaagttcttg gaaagtacca tcctcacggt gattctagcg tctatgacgc
     9001 aatggtcaga atggctcagg attggagtat gagatatatg ttggtggatg gtcaggggaa
     9061 ctttggttca atagatggag actctgcagc cgctatgagg tatacagagg ctaaattccg
     9121 aaaaattgca gaagaggtga tggctgatct tgacaaaaac actgtcgata tgaagccaaa
     9181 tttcgatgaa tcacttcttg aaccaacagt tatgccttca aaagctcctc ttcttttact
     9241 caatggagca tcaggaatag ctgtaggtat ggctacaaac atgccacctc acaatttggg
     9301 tgaagtagtg gatgctattt gtgcttatat cgacaataat gaaataacca ccgaagaact
     9361 gctcaaatat cttaagggtc ctgattttcc tactggtggc attatccagg ggactcaagg
     9421 aataaaggac tattttgaaa caggaagggg tagaattatt gttcgtgcta aaacagaaat
     9481 tgaggtaaat gatagcggga gagagactat tgtggttact gagataccat atatggtcaa
     9541 taaacgtgaa atgatagaga aaattgcaga acttgtagag aataaaaaaa ttgaaggcat
     9601 tgcttatata aatgacgaga gtgacagaaa ggggatgagg attgtgataa aactgaaaat
     9661 gggagcggtc gcaaacgttg ttttaaacat gctcttcaag tacactcaaa tgcagtcaag
     9721 tttttctgtc aataatattg ctcttgtaga tggacgtcca aggattttaa gtgtaaagca
     9781 aattatcaaa tattatgtca gacacagaca tgatgttatt cttcgtcgtt caagatttga
     9841 acttgaacag gcagaaaaaa gagctcacat acttgaaggc ctgttaaagg ctcttgattt
     9901 tattgatgaa gttattgcca tcattagagg ctcaaaaaga gtagaagatg caaggaactc
     9961 tcttatggat aggttctctt ttaccgaaca acaggcatct gccattgtgg agatgagact
    10021 gcgtcaactg acaggtcttg aaagagaaaa attgcaaagt gaatatgaag aacttgagaa
    10081 acaaattgca tatttaaatc aaattctttc acaagtatct ctccagatgc agataatcaa
    10141 ggatgagctt caggaactaa aaaccaaata tgcagatcca aggagaacaa tgatagaact
    10201 ttctgaagat gagtttaatc ccgaagattt ttatgcagat gaagatgtta tcattactat
    10261 ttcacatctg ggatacataa agaggacgcc tctcaatgaa tataggactc agaacagggg
    10321 aggtgttggt gttaaggggg gagcgaccag ggatgaggat tttatcgaaa acatctatgt
    10381 ggccaacatg cacagcacta tgcttttctt tacaaaaaaa ggtaagtgtt actggcttaa
    10441 agtttttgat cttcctgaag gctccaagat atcaaaagga cgtgcgatac agaatgttct
    10501 taatattgaa cctgatgaca aagcgtgtgc ctttattaat gttcacaatc tttcagacaa
    10561 ggaatatgtt aacaataact atatagtact ttgtacaaaa aggggaatag tcaaaaaaac
    10621 ctctcttgag gcatattcaa gaccaagagc caatggagtt aatgctatca ctatcaaaga
    10681 aaatgatgaa ctactcgagg caactctcac aaatggaaaa aatgagattc ttctcgcatc
    10741 aagggaaggt aaatgtgtga ggtttaatga aagttgcgtc agagctatag gaagaaccgg
    10801 tgctggggtc aagggtataa atattagtga agacaatgaa gttattggta tgatttgtgt
    10861 tgacacagat actaaaggca gtggtgaaac aaaacatgtg cttgttgtga gtgaaaacgg
    10921 ctatggcaag agatcattac ttgatgacta cagagtggtg aacagaggcg gaaagggtgt
    10981 taaaacactg agtattactg aaaaaacagg taaattgatt gccattaaag atgtttctga
    11041 tgataatgat ttaatgatta ttaccaaatc tggtcttaca atcaggcttg aagtatcttc
    11101 aataagagtt tcaggaagag caactcaagg cgttaaattg attaacatca aagagggaga
    11161 tagcattgcc gctgtttgtc gcgttaacaa gagcgaagaa aaacctgttg aaacagaaga
    11221 acttgaatga acataaagct atagaaatca ataattaaat ttattaacaa caatcaatac
    11281 caaaatatta caatgaaaaa aatattatta gcattgacat tgcttttttc actgcaattg
    11341 gctgcccaaa ccaaagaggt agcagatgcc ataaaaaatt atgaaaaggc acttaaagag
    11401 tcagctgatg ccaagaaaag tactaatcct gcaacttgga taaagcttgc agacgcttgt
    11461 gctgcagtct atgacacacc tgttaagtcc atttggaacg gggcttcaag aaatgaaatt
    11521 aaacttcttc ttaaagatca gaggattatt tcaactgaag acagaacagt tttagatgtc
    11581 aaatatacag tcgacattta tgatgataag gaactgtatt acaatcaaga tggcacactg
    11641 gcctgttttg ttcagactaa gaactatatt accggtaatc ctattgacga tggagtagca
    11701 gctcttttaa aagcagctga actcgatgtt aaaaaagcta aagccaagga tattacagac
    11761 agactgttag caatgaagaa tcgttatcag actgaagcaa tgacagctta tacacttggt
    11821 gattttaaga aagcgtctta caattttgaa ggttgtgtaa acattagttc aaatccgctt
    11881 attaatcagc ctgatacagt tatgatgtac tatacaggtc tgacagcctt aatggccaaa
    11941 gaatacgata gagctgtgaa atttttacaa atgtccattg aaaatggtta tgacgcaaag
    12001 ggtgatatat actcttctct cgcagaagca tataaatctt caggaaacgt agaaaaggca
    12061 aaagaagtgc ttcaagcagg ttttacaaaa tatcctacaa atcagagcat acttgttatg
    12121 cttattaaca gttatcttga gtcaaatgac gatccaaaca aggttttaac tcttatatcc
    12181 caggctcaaa aaaacgaacc taataatgca agtctgtact atgccgaggg taatgtgtgg
    12241 aaaaatctga aaaacattga caaggctatt gaatgttaca aaaagtctgt agagattgac
    12301 aagtcgtact attttggctc attcgccatt ggagcagcct attatgacag agctgtagag
    12361 atacaggccg cagctactga agaggttgat gacaataaat acgaagaact tgttaaacaa
    12421 cttgaagaga atctaatggc tgccatagaa ccttttgaga attgttttgc atctacatcg
    12481 gataaggaag taaaacttgt tgttgcagag tatctcaaaa atatatattt ccgtttcaga
    12541 gagaagggag acgactacaa agcaaaactt gaaaaataca caaactacct gaacgaaaac
    12601 aagaagtaaa ttaaataatt ttgtttgaaa ttaaagaggg tgttcaaaaa gcaatttttg
    12661 gtccctcttt tttattttct gatttgggaa gctcacctac agacacaaaa gttaaaaaac
    12721 agcagtgctt attggtgagc ttcccatttc tatggttgaa taatatataa aaacagaggc
    12781 tgccttcttt aattattgat agatatctct tttaaaatcc attgagcatt tgcatactta
    12841 tcaattaaaa aaaggacata tcttatatcg agacagatat tcctgcacat atcaggatcg
    12901 aaggcaatat cgctcatggt tccttcccat actcggtcaa aattaattcc tattaattca
    12961 cccttcccgt taatgacagg acttccggaa tttccgcctg atgtatggtt attggcaata
    13021 aaacagacag gtactgtacc atttatattc catttgccat agtctttatt tttgtgaata
    13081 tctctcaatg tttgtggaat attgtaatca taaatttgtg gattgtcttt ttctattata
    13141 ccttccaagg tagaataagg tttgtagtat attccatcag caggcctata accagacact
    13201 ttaccgtatg caactctgag agttgagttt gcatcgggat aaaaatcttt ctttttgtca
    13261 aattccatct gacctttcat gtactttctg tacagaagtg tgatttgatt gtttaacctg
    13321 ttgtattcag gctttatgtc atcatcaaat ttttgtttaa aggcgaagta catttcaaca
    13381 gcaggatctt taagaattgt ggagattgtg acagaatctt gcaatatttt catgcattga
    13441 gattgattgg caaatattga attatcaaat acataatcgg caaatttata tatatctcct
    13501 tcatattcac gcatctttga tataaagtat tcgggttgaa attttaaagg tatatttttt
    13561 ctatattcat caattaacaa aacaaagcat tttttgtcta tcggcagatg atagtcttta
    13621 aaaaaaagtt caattatgtt ttttactgaa tccttatctt catttttaat atttccgtct
    13681 ttacccttaa aacaggttaa cagtgaaccg gtaaacttca atatctcatt tgaattaatt
    13741 gcttcgttat tatagtcgtt agcaaaagaa tatggctcaa gcattttata taaagaatcc
    13801 agactcgata caatattttt atattcatct ctgttctttg cccattcatt gaaacgtttt
    13861 tcatactcag ttttcttctc gacgatattc attcttttaa ttccattcat ctctccctgc
    13921 cattttttcc atgaattggc aacgttagca tttttggccg catacattat tcttatttct
    13981 tgattttgcg acatatactc tttttggaca tccaacctta gtgtccgaag tttaatcttg
    14041 tgaggatttg aaacttcaga gatgtattta actgcgctgc tcattaagta ttcctgagtt
    14101 ttgccgggga agccatatac aagagtaaaa tctccttcat ttataccggt tgtagatata
    14161 gagaaatatt ttttaggtct gtaaggaaca ttattttttg aatattttgc aggtctatta
    14221 ttcttgtcgg cgtatatcct aaaaatagaa aagtctccgg tgtgtcttgg ccacatccaa
    14281 ttatctgtat cacccccaaa tttcccgata gaagatggtg gtgcaccaac aagtctaaca
    14341 tcataaaatg tttcatatat aaaaagaaaa tactggttac cataatacaa agattctatg
    14401 ctgcctttgt aagtagttcc ttttgttgca ttttcgatga ttcttgccga attctcagca
    14461 actatttttt gtctttcaga ttcagacatt gaaggaagat atgatttcag aacagcatca
    14521 gtaacatcct ccatttttac aagaaaactt actgtaagat tatcatttgg cagttcttct
    14581 tccatgttca ttgcccagaa tccgtctttc aggtaatcgt gttctactgt actgtgagac
    14641 tgtatgtaac tgaatccaca gtgatgattt gtgattaaaa gccctttttc agaaattaat
    14701 tctcctgtgc aacctc
//
LOCUS       DAOTQL010000029        14491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_3982_length_14491_cov_5.364921, whole genome shotgun sequence.
ACCESSION   DAOTQL010000029 DAOTQL010000000
VERSION     DAOTQL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_14491_cov_5.364921"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..671)
                     /locus_tag="P5293_03055"
     CDS             complement(114..671)
                     /locus_tag="P5293_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR48912.1"
                     /translation="MKKEEENILIDKIKKGDKSLFDKLVIEYSPLVISVIRGILCNRE
                     DAEEIAQDVFVKAFFSLKKFRGDSSFSTWIYRIAYNMAISKARQKKKIFIQIDKLSIP
                     DKSFTTAEVESKEKKIQKLLNDILKNLTPSDRFIIISFYMHRKSIAEISVITGLSESN
                     VKVRLHRMKKKLNLILKEKLEVSYG"
     gene            793..1203
                     /locus_tag="P5293_03060"
     CDS             793..1203
                     /locus_tag="P5293_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48913.1"
                     /translation="MLDFITVPLVTGICIFGFYKLVELFVRKRERIMLIERISQLENI
                     DSNKIDLSRIFGNEPYRNGRFVALRAGMLLIGVGLGLLVGFIIVYNTFGPLEGDFYDR
                     SNGMAELIYGSTTLLFGGLGLIAAFIIEHKLRKG"
     gene            complement(1281..2354)
                     /locus_tag="P5293_03065"
     CDS             complement(1281..2354)
                     /locus_tag="P5293_03065"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HRR48914.1"
                     /translation="MKPTKKDLLKETIKHIDISKIDAIPFIDAMRDMSFTSRDTANAA
                     DILQLMIKDKECSNWLIIAGSTSAGGCMQVYVDMLRYNMIDVVVSTGASIIDMDFFEA
                     LGFKHYKGSQFINDTYLRENYIDRIYDTFIDEEELQKCDATIKAIADSLEPRPYSSRE
                     FIHEMGKWLTKNSVKKNSLIQTAYELNVPIFVPAFSDCSAGFGLVMHQIERMKEGKPY
                     LSIDSVADFRELTEVKLSSKNSGLFMIGGGVPKNFAQDTVVCAECLGFEVPMHKYAVQ
                     ITVADVRDGACSSSTLKEANSWGKVDTANEQMVYAEATTVVPMIAAYAYQKGNWKNRE
                     RRNHALIFKNGTQGSGILLKSNC"
     gene            2452..3603
                     /gene="lysA"
                     /locus_tag="P5293_03070"
     CDS             2452..3603
                     /gene="lysA"
                     /locus_tag="P5293_03070"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRR48915.1"
                     /translation="MFDTQTLRRFETLDTPFYYYDLSLLRQTLKVYTFYTRKYNYSAH
                     YALKANADEKILSIIKEAGLGADCVSGNEVSLALKIGFPPEKVVYAGVGKSDKEIITA
                     LKGNIFSFNCESIPEIEVINALASSMGRIANISLRINPNIDAHTHKYISTGRAQDKFG
                     ISPWMFDSVLETIKRSANIRLIGLHFHIGSQITDLTVFKLLCERVDSLMHWFIDRDVT
                     ISNINLGGGIGVDYSNPDEYPIADFEDYFKIISQNLKVLPGQRVHFEPGRSIVAQCGT
                     LISRVLYVKIGKGRKFVILDAGMNDLIRPALYQASHAIQNLSSTGRAMRYDVVGPICE
                     SSDVFGKNIKLPKTQRGDIIAIRSAGAYGQVMAMRYNQRDFAPQYYSET"
     gene            3607..4215
                     /locus_tag="P5293_03075"
     CDS             3607..4215
                     /locus_tag="P5293_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRR48916.1"
                     /translation="MSRVSYKEKYVIQLYRLFRKKGLRLTMDQIASELNLTKKTLYNN
                     FKSKEELMRIVMQHVLSDIEFRMIISLTQGKNAIEALYNTSSMMNKALEEIGPLLLND
                     TATYLPDLKLLNHTDRMSFYSRIIFENLERGISESLYRDDLNPKLVTIFFTSAMAKIY
                     SWNGSYRYLKDPYRFHSELIRYHLEAIVNDEGRLVLRQYLKK"
     gene            4243..5574
                     /gene="ffh"
                     /locus_tag="P5293_03080"
     CDS             4243..5574
                     /gene="ffh"
                     /locus_tag="P5293_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRR48917.1"
                     /translation="MFENLIDRLEKSFKILKGEGKITEINVAETLKEIRKALLDADVS
                     YKIAKNFCDEVKQKALGQNVLKSVSPGQMMTKIVHDELTRLMGGEAVDISVKGNPGIV
                     LVAGLQGSGKTTFSGKLALNCKSKRGLKCMLVACDIYRPAAIEQLKIIGNQVGVPVYS
                     EEGNNNPVQIAKNAIQKARQDGFSLVIIDTAGRLAVDEQMMTEIEAIKKAVNPTETLF
                     VVDSMTGQDAVETAKTFNERLNFDGVVLTKLDGDTRGGAALSIRAVVDKPIKFISAGE
                     KMEALDVFHPSRIADRILGMGDVVSLVEKAQEQFNEEEARKLQKKLAKDQFTLWDFYN
                     QIQQIKKMGNIKELASMIPGMGKALRDIDIDNSSFKSIEAIIQSMTPEERENPAILNG
                     SRRKRIATGSGTTIADVNRLIKQFDDMRKVMKNMSTGGKSAMRAVGAMRGR"
     gene            5594..6460
                     /gene="folD"
                     /locus_tag="P5293_03085"
     CDS             5594..6460
                     /gene="folD"
                     /locus_tag="P5293_03085"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRR48918.1"
                     /translation="MIILDGKKTADEIKREISAKVEQIVKDGGKRPHLAAILVGEDGA
                     SQTYVNNKEKTCFQVGFKSTVIRMPESTTQQRLLEEIKRLNEDEDIDGLIVQLPLPKH
                     IDEQKIIEAIDPEKDVDGFHPMNVGKMVLGMPSYISATPGGILELISRYDIKTSGKHC
                     VIIGRSNIVGRPLANLLSQKTQPGDCTVTICHSHTKDIKEITLQADILIAAIGKPGFV
                     RGDMVKSGAVVIDVGITRVPANNKSGFKLAGDVLFDEVAPKASFITPVPGGVGPMTII
                     SLLKNTLKASGN"
     gene            complement(6474..8909)
                     /locus_tag="P5293_03090"
     CDS             complement(6474..8909)
                     /locus_tag="P5293_03090"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR48919.1"
                     /translation="MYNKLAYLLIALLINTNLLAAKDNNIKGKIIEKENGKALPYATI
                     SVQDNNLKIIGGTTTNEEGQFTINNIEIDSFMVKISFIGFKDTTINVNMDNLTDNNLG
                     IIALSPDASTLKAAVITEKIPVIEQKVDKIVMNVSEAVSTQGSSTLDILRKAPGISVD
                     PSGNILLNGNIVQIWIDNRPSNLTGADLEALLSSTDGSTIDKIEIIAHPSAKYDAEGS
                     SGIVNIKTKRNFAKGLSGSLRATYGASPFESELYNSVSSSINLAYRGNKSNTTVTYSP
                     KFDESYDKFTSVTNLDEAQMLKGITLNKYINKRFNFKVSHDYYINKQNIIGVIISGLN
                     RKNDENTDNDITGSKLYKNGTLIKTINTSIDGKSNFNNIYTNLNYTHIFKDNHEVTVN
                     ADYGYYDIGSDSHQENKYFNVIEGTDDPSNIFNSYSRQYLNIVSTKLDYEQLVLNKIM
                     LEVGGKWAQSQTDNNLKREDFIDNVWVKNNLLSSKFNYKENIVASYISAGIQFGPKLS
                     AKAGIRAEYTISDGDWISADTMTQKKYLDIFPTLFFGFNPNKNTTIAATYNLRIQRPN
                     FSQLNPFRIYVDANTAVEGNPNLLPQYTHQASLSLIYKHFLSLGFYGQFNTKSIIQNT
                     KFNSVTGEKLLIWENFGNLNLYGGVLNVTELPVTKWLTINSGLLVAQVKSKTDGYEKK
                     SLFSSINMNAEFSLPKDTKIELSGIFQSGIPYGFFKVKPQGDISLGIKKGVLNNRGTL
                     ILNCSDLFLTQNSRAVLATDIIENYRFKSQWKSREISISFLYHFGNGIATKARKVGES
                     EEAKRAESDNAKK"
     gene            complement(9000..9353)
                     /locus_tag="P5293_03095"
     CDS             complement(9000..9353)
                     /locus_tag="P5293_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRR48920.1"
                     /translation="MEFNEHKPIYLQIADNLCDKILSEEWKPEERIPSVRELGMLLGV
                     NPNTIMRVYDYLQSYCIIFNKRGVGYFISKDAKEIIISVQKQQFIQEELPKILKRMKL
                     LGINPSELLKNNIDN"
     gene            complement(9357..10151)
                     /locus_tag="P5293_03100"
     CDS             complement(9357..10151)
                     /locus_tag="P5293_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48921.1"
                     /translation="MNEIFSFKRYSKLLTKELKERRIIILKMAGIVSIIVLGIWLTSI
                     LLKGGTGVSPSFRLSMMLIILVITMIAAPFNLYKSYNHPKKGIDYALLPASVYEKFFS
                     MLTIVVIITPITIFAALMLTDFVLSIITPSVFYGFAFSGKIDEISFSSIYDVLIVQWA
                     FIFGNFLFKKSKITKTISLGIGIYLVLGLILASTVKSVVFGQGDNSVNFSMEINNLSD
                     FLANNSYDEENKIRLLLYTTKTIVYYIIPIGCLGGTLLKMKNQQYY"
     gene            complement(10161..10988)
                     /locus_tag="P5293_03105"
     CDS             complement(10161..10988)
                     /locus_tag="P5293_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR48922.1"
                     /translation="MLKIDNLSFSYSKHEVFKGITLELDCGKIYGLLGQNGVGKTTLL
                     KIISGLLRPSSGTCKLLNHFTYKREPSFLQELFFIPEDFAGIDLEIYKYADLRGMFYP
                     RYDKNKFEKTLIDFEVDKKVKFTKLSFGQQKKAIISFALATNTKLILMDEPSNGLDIP
                     SKTQLRKIIAQASTDESCIVLSTHQVRDLENIIDPIIILDTNDVLLNASIEEISKKLK
                     FELRESLDEKALYNEPSLGGYISVYPNTDNIETKVNIEALFNTVLTNKKIIQLILNK"
     gene            11108..12682
                     /locus_tag="P5293_03110"
     CDS             11108..12682
                     /locus_tag="P5293_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRR48923.1"
                     /translation="MKIKILVFLFIFFQTVNLYAQRLYSNEDKLKKTLYCLADDKMAG
                     REAGTEYEFNAAGYIVETLYSYGFKPVFNNNPLVKFKLGKGRISGNNSILEINGKDFI
                     LKQDFFVPPFSSLSNVSGRLSTVPEAESVLLLKSSTDSIRLKVASFRDLGVKSLIYYS
                     GSELDTLKYMETVPTSIPVVQVTEECAKLLQEASGSIIHIYTDVKSVDLMSNNVFMSK
                     GGRLDKPYILIGAHYDHLGFGGEGSMKKGINAIHNGADDNASGVAAMMEIARILRNSG
                     DGLYDFVFVAFGAEEKELVGSGFLADTLKRLGRLPVLMINLDMVGRLVDNKLQISGMG
                     TFKGADSLLQNVNQKFNFMLLTTKDGYGPSDQSSFVSMGIPVLYFTTGVHSDYHTPDD
                     DPDKINYSGLKMITDFVASLLRDLSMSGMKMEYVNLPPPPSMMSKKSLKVTLGLIPDF
                     TYDVGDGFKVGPVTEGKPAAMAGLKAGDIITAINSKKVNNVYEYMSRLSELKIGDKIV
                     LDVRRDGKELKLIVQL"
     gene            12685..13602
                     /locus_tag="P5293_03115"
     CDS             12685..13602
                     /locus_tag="P5293_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48924.1"
                     /translation="MYNIKIKKILYWFGAVLITLASVVYQRATGPTNPKKYSFNLAGK
                     TYEVKLPRSLETEITPEQAKGDYEALGKISCFDVEVKGASDSMQMTVFFRRYPSQDTI
                     SIVKSERSGDIFTICIPSQPPAGKLEYFVEFSDRNDHITLGYGEYLTIRFKNLVPVWV
                     LLPHVLLMFIAMLLSTYVGIIAFDKTQKTRVPVMLLMISLFLGGLLFGPIVQKYAFGE
                     FWTGWPFGHDMTDNKTLAAMLVWMVAILGNRSKKKRWWYVVAAIFMLLIYSIPHSTSG
                     SEFDYSKKEVVTGESVNICEHPVNSDLRC"
     gene            complement(13559..>14491)
                     /locus_tag="P5293_03120"
     CDS             complement(13559..>14491)
                     /locus_tag="P5293_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRR48925.1"
                     /translation="SFPLYYSTSFGGAYWLWITILFLFVIQAVSYEYRNKAGNLLGAK
                     TYEVFLFLNGTLGTILLGVAVATFFTGGSFVMDKMSITETAKPSISYWTSSWHGLEAI
                     TVPFNFLMGIVVFLAARTLGLLYVIMQIDSESLKSKCKSRVKATGIGFVVAFILFLVS
                     LFTRVGYSVNPESGLITPVQYKYFFNMIELGWPAVMLLIGVVLVLWGLGNNYYGKGKY
                     SFYLASAGVVFSVWSILICAAFNNTAFFVSTYNLQDSLTLYNSSSSLFTLKVMAWVTL
                     LLPFVIAYIVYVWRALTRKNISIEDLNSQDAHKY"
BASE COUNT         4576 a         2351 c         2683 g         4881 t
ORIGIN      
        1 tttttctatc ctcaattgtg atttttctca tcacttcatc ttcgaatccc aattcttttt
       61 tttcgtaatc ttttgacaaa gatttaatga gttttaataa tatatcttct tgctcatcca
      121 tagctcacct ccaatttttc tttaagaatt agatttaatt tctttttcat tctgtgcaat
      181 ctaactttta cgtttgattc gctcaaacct gttattacag aaatttctgc aatacttttt
      241 ctgtgcatgt aaaaagaaat aattataaag cgatcagatg gagtcagatt cttaagaatg
      301 tcatttagta acttttgaat tttcttttct ttagattcaa cttctgcagt agtgaaactc
      361 ttatcaggaa ttgataactt atctatctga ataaaaattt ttttcttttg tcttgcttta
      421 gatatagcca tattataagc aattcggtat atccatgttg agaaggaaga atcaccacga
      481 aattttttta atgaaaaaaa ggccttgaca aaaacatctt gtgcaatttc ttctgcatct
      541 tccctgttac aaagaatacc cctaataact gaaatgacca atggagaata ctcaattaca
      601 agtttatcaa atagtgattt atctcctttt tttattttat caatcagtat attttcttct
      661 tcttttttca tttgacatat tgacgcaaat gaatattcaa ggttacaact aatattatat
      721 ttttttgttt aaccattttg taacctccat taaaagattt cgtcaaaaat ataaatttaa
      781 ataatataag ttatgcttga ttttattaca gttccattag ttacaggaat atgtatcttt
      841 ggattttaca aactcgtcga acttttcgtt agaaagagag aaagaataat gcttattgaa
      901 cgtatatctc aattagaaaa tattgatagc aataaaattg atctttctcg aatttttggt
      961 aatgagcctt atcgtaatgg tcgttttgta gcattgagag caggtatgtt gttaataggc
     1021 gttggattag gccttcttgt gggctttatc attgtttata atacttttgg cccgttagag
     1081 ggagattttt acgatagatc taatggaatg gctgaactta tttatggttc tacaacattg
     1141 ctgtttggtg gattgggtct tattgctgca tttatcatcg agcataaact tcgtaaaggt
     1201 tagataaatg actctattaa taataaaagg agcgtcttat tgtggataat caagacgctc
     1261 cttattatat cagaaaagtg ttaacaattg ctttttaaaa gaattccaga gccttgagtg
     1321 ccatttttaa aaattaaagc gtgatttctt ctctcgcggt ttttccagtt tcccttttga
     1381 taagcataag cagcaatcat aggaacaaca gttgttgctt ctgcgtatac catctgttcg
     1441 ttagcagtat caacttttcc ccatgagttt gcctccttga gggtagatga cgagcaggct
     1501 ccatctctga catccgcaac agtgatttga acggcatatt tgtgcattgg aacttcaaat
     1561 cccaagcatt ctgcacatac aacagtgtct tgtgcaaagt tctttggaac accacctcca
     1621 atcataaaca atccactgtt ttttgaagat aatttaacct cagtaagttc tctaaagtcg
     1681 gccacagagt caatggataa gtacggttta ccttctttca ttctttcaat ttgatgcatg
     1741 accagaccaa aaccggcact gcaatcacta aatgcaggaa cgaatatggg cacattcagc
     1801 tcataggctg tttgaataag agaattcttt ttaacagaat tttttgtcaa ccatttaccc
     1861 atttcgtgaa taaattctct tgaagaatat ggacgtggtt cgagagaatc ggcaatcgct
     1921 ttgatagttg cgtcacactt ttgaagctct tcttcgtcga taaatgtgtc atatattctg
     1981 tcgatatagt tttctctaag ataagtatca ttaataaact gagatccttt gtagtgttta
     2041 aaacccaaag cttcaaagaa atccatatca attatggatg ctccagttga aacaacaacg
     2101 tctatcatat tatatcttaa catatccaca tagacttgca tgcatcctcc tgcagaagtg
     2161 ctgccggcaa ttatcagcca atttgaacat tctttgtctt taatcattag ttgaagaata
     2221 tccgcagcat ttgcagtatc tcttgatgta aaggacatat ccctcattgc atctataaat
     2281 ggaatagcat ctattttgga gatatcaatg tgtttgattg tctcttttaa aagatctttt
     2341 tttgttggtt tcatttttat atataataat tttgtggcaa agatagataa tttaagttaa
     2401 gaacaagttt tttgctattt ttgcagcatc ttacaatata ttaattacta catgtttgac
     2461 acacaaactt taaggagatt tgaaacttta gatactcctt tttactatta tgatctctct
     2521 ctcttgagac aaacattgaa ggtatatacc ttttatacaa gaaaatataa ttatagtgct
     2581 cactatgcgc taaaggctaa tgcagatgag aaaatccttt cgattattaa ggaggcagga
     2641 cttggggcag actgcgtaag tggcaacgag gtttctcttg cacttaaaat cggctttcca
     2701 ccggaaaagg tagtttatgc aggagtcggg aaatcggata aggaaataat tactgctctt
     2761 aaaggcaaca ttttcagttt taattgcgaa tccatacctg aaattgaggt aattaatgcc
     2821 ttagcctctt caatgggaag aatagccaat atttccttga gaataaatcc gaatatagat
     2881 gctcatactc acaagtatat tagtacagga agagctcaag acaagtttgg gataagtcca
     2941 tggatgtttg actctgttct agaaaccatt aaaagatctg caaacatacg tcttattggt
     3001 ctgcattttc atataggatc tcagataaca gaccttactg tcttcaaact tttgtgtgaa
     3061 agagtggatt cactaatgca ctggtttatt gacagagatg tgacaataag caatataaat
     3121 cttggtgggg gtattggtgt ggattacagt aatcctgatg aataccctat cgctgatttt
     3181 gaagattatt ttaagattat atctcaaaat ctcaaagttt taccggggca aagagtacat
     3241 tttgaaccag gtcgctcgat cgtagctcag tgtgggaccc tcatttcaag agttttgtat
     3301 gttaaaatcg gaaaaggaag aaagtttgtt attttagatg cagggatgaa cgatttaatc
     3361 cggccagccc tttatcaggc atcacatgca attcaaaact tgtcttcaac aggaagagct
     3421 atgaggtatg atgtagtagg ccctatatgt gaatccagcg atgtttttgg aaaaaatata
     3481 aagttgccaa aaactcaaag gggagatatt atagcgataa gaagtgccgg tgcgtatggt
     3541 caggtgatgg caatgaggta taatcaacgc gattttgctc ctcaatacta ttctgaaact
     3601 tgatttatga gcagagtcag ttataaggaa aaatatgtaa ttcagctata cagacttttc
     3661 agaaagaagg gtttaagatt aacaatggat caaattgcat cagagttgaa tcttaccaaa
     3721 aaaactcttt ataacaattt caaaagtaaa gaagaactca tgcggattgt tatgcagcat
     3781 gtactaagcg atattgaatt tagaatgata atttctctga ctcaaggcaa aaatgcaatt
     3841 gaagcacttt ataacacaag ttctatgatg aataaggctc ttgaagagat tgggccttta
     3901 ttgctaaatg atactgcaac ttaccttcct gaccttaagt tgttgaatca cactgacagg
     3961 atgagttttt attcaagaat tatttttgaa aatcttgaga gaggaatttc tgagtcgctt
     4021 taccgggatg atttgaatcc caaattagta actattttct ttacttctgc aatggcaaaa
     4081 atttattctt ggaatggttc ttatcgttat ttaaaagatc cctataggtt tcattcagaa
     4141 ttgataagat atcacctaga ggctattgta aatgatgagg gtagactagt tctgagacag
     4201 tatctaaaaa aatgactatt tttgaaacgt aaaatgtgaa agatgtttga gaatttaata
     4261 gaccgattag agaaatcatt taagatactc aagggtgagg ggaaaattac tgagataaat
     4321 gtagctgaaa cactaaaaga gatacgtaag gcattacttg atgctgatgt cagctataaa
     4381 atagccaaaa atttctgtga cgaagttaaa caaaaagcct tggggcagaa cgttcttaag
     4441 tcggttagtc ccgggcagat gatgaccaag atagttcacg atgagctcac tcgtcttatg
     4501 ggtggtgaag ctgtagatat ctcagtaaaa ggtaatccgg gtattgttct tgttgcaggt
     4561 ttgcaaggtt caggtaaaac aacattttca ggtaagctgg cacttaattg caagagcaaa
     4621 agagggctta agtgtatgct ggttgcatgc gatatttacc gtcctgctgc gatagaacaa
     4681 ttgaagataa ttggaaatca ggttggtgtg ccggtataca gcgaagaggg taacaacaat
     4741 cctgttcaaa ttgcaaagaa tgcaatacaa aaagccagac aggatggatt ttcacttgtg
     4801 ataatagata cagcaggacg tcttgctgtt gatgaacaaa tgatgacaga aatagaagct
     4861 atcaaaaagg ctgttaatcc tacggaaaca ctttttgttg ttgattctat gactggccaa
     4921 gatgcagttg aaactgccaa aaccttcaat gaaaggctga attttgatgg tgttgtactc
     4981 acaaaacttg atggagacac gcgtggtgga gcagcactgt caataagggc tgtggttgac
     5041 aagcctatca agtttatatc tgccggagaa aaaatggagg ctctcgatgt ttttcatccg
     5101 agtcgtatag ctgacaggat tcttggaatg ggagatgtag tttctctagt tgaaaaagct
     5161 caggagcagt ttaacgaaga agaggcaaga aagttacaaa aaaaacttgc caaagaccag
     5221 tttactcttt gggattttta caaccagata caacagataa aaaagatggg taatatcaaa
     5281 gagttggcat ctatgattcc cggtatgggt aaagctctgc gagacattga tatagataac
     5341 tcttctttta aaagtattga agccattatt cagtcaatga ctcctgagga acgtgaaaat
     5401 ccagctattt tgaatggaag tagacgcaag cggattgcta caggaagtgg gacgactatt
     5461 gcagacgtta acaggttaat caaacaattt gacgatatgc gcaaggtaat gaaaaacatg
     5521 tcgactggag ggaagagtgc tatgagagct gtgggggcta tgcgtggcag ataaacagtt
     5581 aattacaaag gtaatgataa tactagatgg taaaaaaacg gcggatgaga taaaacggga
     5641 gatttctgca aaagttgagc aaattgtcaa ggatggaggc aaaagaccac atcttgcggc
     5701 tattctggtt ggagaagacg gtgcttctca aacctatgtg aacaataagg aaaaaacttg
     5761 ttttcaggtt ggattcaaat ctacagttat aagaatgcct gaatcgacta ctcaacaaag
     5821 acttcttgaa gagattaaaa ggcttaatga ggatgaagat atcgatggcc tgatagttca
     5881 gctacctttg cccaaacata ttgacgagca gaaaataata gaggctattg atcctgaaaa
     5941 agatgttgac ggatttcatc ctatgaatgt tggtaaaatg gttctaggca tgccctcata
     6001 catttctgct actccaggag gaatattgga acttatatca cgatacgata ttaaaacttc
     6061 tggaaagcat tgtgttataa ttggtagaag caatattgtc ggtaggcctt tggctaatct
     6121 tctttctcaa aaaactcagc ccggggactg tacagtcact atctgtcata gtcatactaa
     6181 agatattaaa gagataaccc tacaagcaga tattttgatt gccgctatag gaaaaccagg
     6241 ctttgtcaga ggtgatatgg ttaagagtgg ggcagtcgtt attgatgttg gtattactag
     6301 agttccagca aataacaaat ccggatttaa acttgcagga gacgttctgt ttgatgaagt
     6361 tgcgcctaag gcttctttca ttacccccgt tccgggggga gttggtccca tgacaattat
     6421 ttctttactc aaaaacacct taaaagcctc aggtaattga tatctctgaa agtctatttt
     6481 tttgcattat cactttctgc tctttttgcc tcttcgcttt caccaacttt tcttgctttt
     6541 gttgcaattc cgttaccgaa atgatagagg aaacttatgg atatttctct gcttttccat
     6601 tgtgatttaa atctataatt ttcaattatg tctgttgcaa gaactgcacg gctgttttga
     6661 gtaagaaaca gatcactaca atttaatatt aaagttcctc ggttattaag cactcccttt
     6721 ttaatgccta aggatatatc tccttgaggt tttaccttaa aaaaaccata gggaattccg
     6781 ctttgaaata taccggatag ctcaattttt gtgtcttttg gaagagaaaa ttctgcattc
     6841 atgttaattg acgagaataa tgattttttt tcatatccat ctgttttact tttaacttga
     6901 gctaccaaaa ggccagaatt tatggttaac cacttagtaa caggaagttc tgtgacattt
     6961 aaaacccctc catataaatt tagatttcca aaattttccc atatcaatag tttttcacct
     7021 gttacagagt taaattttgt attctggatg atagacttag tgttaaattg tccataaaaa
     7081 cccaaactga gaaaatgctt gtatattaaa gaaagacttg cttgatgagt atattgtggt
     7141 aataaatttg gattgccttc tactgcagtg ttggcatcga catatattct gaatggattg
     7201 agttgactga aattgggtct ctgaattctt aaattataag tagcggctat agttgtattt
     7261 ttatttggat tgaacccaaa aaacagagtt ggaaatatat cgagatattt tttttgtgtc
     7321 attgtatcag cacttatcca gtcaccatcg gaaatagtgt attcagccct tattccggct
     7381 tttgcactca attttggtcc aaattgtatt cctgctgata tgtatgatgc gacaatattt
     7441 tccttgtagt taaatttact acttaataaa ttgtttttta cccaaacatt atcaataaag
     7501 tcctctcttt ttagattatt gtcagtttga ctctgtgccc actttccacc gacttccagc
     7561 ataattttat tcaaaactaa ttgttcataa tccaactttg ttgagacaat gttgagatat
     7621 tgtctggaat agctattaaa tatatttgat ggatcatcag ttccttctat tacattgaaa
     7681 tatttgtttt cctgatgact gtcagaacct atgtcatagt acccataatc agcatttact
     7741 gtaacttcat gattatcttt aaatatatga gtgtagttta aatttgtata aatgttatta
     7801 aaattacttt tgccatctat tgaagtatta atggttttaa ttaaagttcc gtttttgtat
     7861 agtttacttc cagtaatgtc attgtctgta ttttcatcat tcttacggtt taagccactg
     7921 ataataacac ctataatatt ttgcttgttt atataatagt cgtgtgaaac cttgaaattg
     7981 aatcttttat tgatatattt gttcaatgtt atccctttaa gcatttgggc ttcatccaaa
     8041 tttgtcacag aggtaaactt atcgtatgac tcatcgaact ttgggctgta agtcactgtt
     8101 gtgttgctct tatttcctct gtaggccaga ttgattgaac tgcttacact attatacaat
     8161 tctgattcaa aaggagatgc gccataagtg gctcttaaag agccgcttag cccctttgcg
     8221 aaattcctct ttgtttttat attaacaatt ccactgcttc cttctgcatc atattttgct
     8281 gaaggatgtg caataatttc aattttatcg attgtacttc catctgtgct tgaaagaaga
     8341 gcctccaaat cagctccagt caagtttgat ggcctattgt caatccatat ttggacaatg
     8401 tttccgttca aaagtatatt tcctgatgga tccacactta tcccaggagc ctttctcagt
     8461 atgtcaagag tgctgctacc ctgagtcgac acagcttcgc taacgttcat aactattttg
     8521 tcaaccttct gttcaataac tggaattttt tctgttataa cggcggcttt taatgttgat
     8581 gcatctggag aaagcgcaat aatcccaaga ttattgtcag ttaaattatc catattaaca
     8641 tttatggtag tatccttgaa tccaataaag gagattttta ccataaatga atctatctct
     8701 atattattga ttgtaaattg accctcttca tttgttgttg ttcctcctat aatttttaaa
     8761 ttgttgtcct gtacactgat agttgcatat ggaagagctt tgccattctc tttttcaatt
     8821 atcttacctt ttatattgtt gtcttttgcg gctaaaagat tagtgttaat aagtagtgct
     8881 atcagcaaat aagctaattt gttatacata gtaaaagatg ttttatggta cattgttaag
     8941 cataataatt ggttttgtta ttataggacg ataaagaata aagtttgtga catttttaat
     9001 taattatcta tattgttttt taacaattct gatggattta tgccaagtaa cttcatcctt
     9061 ttgagaatct tgggcagttc ttcctgaata aattgctgct tttgtacaga aatgataatt
     9121 tcctttgcgt ctttggaaat aaagtagcca actccacgtt tgttgaaaat tatacagtag
     9181 gattgtaaat aatcatagac tctcattatt gtattagggt ttactcccaa taacattccc
     9241 aattctctca ctgagggaat tctctcttcg ggtttccact cttcactgag tattttatca
     9301 catagattat ctgcaatttg aaggtagatt ggtttatgtt cgttaaactc catacattaa
     9361 taatattgtt gatttttcat tttaagcaag gtcccgccaa gacagccaat gggaattata
     9421 tagtagacaa ttgtttttgt agtatataac aacaatctga ttttgttttc ttcatcataa
     9481 gaattgtttg ctaaaaaatc actaagattg tttatctcca tagaaaaatt aacagaattg
     9541 tctccttgtc caaaaacaac cgatttaact gtcgaggcaa gaattagccc taatactaaa
     9601 tatattccta ttccaagtga aatggttttt gtaatcttac tttttttaaa aaggaaattt
     9661 ccaaatataa aagcccactg cacaattaga acatcgtaga ttgaactaaa gctgatttca
     9721 tcaattttcc cactaaatgc aaaaccgtaa aaaacggaag gagtaataat agaaagaacg
     9781 aagtctgtca gcattagcgc tgcaaaaatt gtgattggag ttattatgac aacaatagtc
     9841 agcatactaa aaaatttttc gtaaaccgag gcaggtaaaa gtgcatagtc aatccctttt
     9901 tttggatgat tgtagctttt ataaagatta aatggtgcag ctatcatagt tataaccaaa
     9961 atgataagca tcatagaaag cctgaatgat ggcgaaactc cagtaccacc tttaagaaga
    10021 attgaagtca gccatatgcc aagtacaata atagatacta ttccggccat tttgagtatt
    10081 ataattcttc tctcttttaa ctcctttgtt aaaagctttg aatatctttt aaaggaaaaa
    10141 atttcattca ttgttttaag ttatttattg agaattagtt gaattatttt tttgttagtt
    10201 aaaacagtat taaacagagc ttctatgttt acttttgttt caatattgtc tgtatttgga
    10261 taaacagaga tgtaacctcc caaagatggt tcgttgtata gagctttttc gtccagagat
    10321 tctcgaagtt caaattttag ttttttactg atctcttcaa ttgaagcgtt taacagcaca
    10381 tcgtttgtgt caagaataat gattggatca ataatatttt cgagatctct gacttgatga
    10441 gtggacaata cgatgcaact ttcatcagta gaggcttgag caataatctt tcttaactgt
    10501 gtttttgaag gaatatcgag gccgttactt ggttcatcca tcaatattag ttttgtattg
    10561 gttgcaagtg caaaagaaat gattgccttt ttttgttgcc caaatgatag ttttgtaaat
    10621 tttacttttt tgtcaacttc aaaatcaatc aacgttttct caaatttgtt tttatcgtat
    10681 cttggataaa acattcctct caaatctgca tatttataaa tttccaaatc aatacctgca
    10741 aaatcttctg gaatgaaaaa caattcttgc aagaaacttg gttctctttt ataagtgaaa
    10801 tgattcaaaa gtttacacgt gcccgatgaa ggtcttaata aaccactaat aattttgaga
    10861 agagtagtct ttccaacccc attttgtccg agtaaaccgt agatttttcc acagtcaagt
    10921 tcaagagtga ttcctttgaa tacttcatgt ttagagtagc taaaacttag attatcaatt
    10981 tttaacataa tatattaaaa ttaattatag tgtattagtg aaatagtaca ctgcaaaggt
    11041 agtaatgatt ttattagttc caaattttat ttacttttgg atctaatttt tttgataaag
    11101 taatttgatg aaaataaaaa tattggtttt tctgtttatt ttctttcaga cagtcaatct
    11161 ctatgcgcaa aggctatatt ctaatgaaga taaattaaag aaaactcttt attgtcttgc
    11221 tgatgataaa atggctggga gggaagccgg gacagaatat gaatttaatg ctgccggcta
    11281 tattgtcgag acactttact cttatggatt caaacccgtt tttaacaaca atccgctggt
    11341 taaatttaaa ttgggaaaag gaagaatttc tggaaacaat tcaattttgg aaattaatgg
    11401 taaggatttt attttaaaac aggatttttt tgttcctccc ttttcttcat tatctaatgt
    11461 ttcaggacga ctatcgacag tgccggaagc ggaatcggtt ttgcttttga aatcctcgac
    11521 tgactcaata agattgaagg ttgcttcatt cagggatctg ggcgtaaagt ctttaattta
    11581 ttattcagga tcagaacttg acactctcaa gtatatggag actgttccaa cttcaatacc
    11641 agttgttcag gttaccgaag agtgtgccaa attgcttcaa gaagcatcag gaagtattat
    11701 ccatatttat actgatgtaa aatctgttga tcttatgtca aataatgttt ttatgtcaaa
    11761 gggagggaga cttgataaac catatatatt gataggggca cattatgatc atcttggttt
    11821 tggaggtgag ggaagcatga aaaaaggcat aaatgcaatt cacaatggtg cagacgacaa
    11881 tgcaagtgga gttgctgcta tgatggaaat cgcaagaatt cttagaaata gcggcgatgg
    11941 tctttatgat tttgtctttg tagcttttgg cgcagaagaa aaagagcttg tgggttctgg
    12001 ttttttggct gatacactta aacggttggg acgattgcca gttcttatga taaaccttga
    12061 catggttggc aggttggtag ataataagct tcaaataagt ggtatgggta cttttaaagg
    12121 tgctgactct ctgttgcaaa atgtaaatca gaagtttaat ttcatgttgt taacaacaaa
    12181 agacggatat ggcccttctg atcaatcttc gtttgtctca atgggtattc cagttttata
    12241 ttttacaaca ggggtacatt cagattatca cactcctgat gatgatccag ataagattaa
    12301 ctattcagga cttaaaatga taactgattt tgtggcttct ttgttgagag atttatcaat
    12361 gagcggaatg aaaatggaat atgttaattt accaccgcct cctagtatga tgagtaaaaa
    12421 atctttaaag gtaacactag ggcttattcc ggattttact tatgatgtag gagacgggtt
    12481 caaagtcggc cctgtcactg aaggaaagcc tgcagctatg gccggtttaa aggcaggaga
    12541 catcattacc gcaattaatt caaagaaggt taacaatgtt tatgagtata tgtcaagact
    12601 ttcagaactc aagattggag ataagatagt tttggatgta aggagagacg ggaaggaact
    12661 taaacttata gtgcaattgt agttatgtat aatatcaaga ttaaaaagat tctttactgg
    12721 tttggcgcag tgttaataac attagcttct gttgtgtacc agagagcaac gggtccgaca
    12781 aatcccaaaa agtattcttt taatttggct ggcaaaacat atgaagtgaa acttcccagg
    12841 agtcttgaga cagaaatcac acctgaacag gcaaagggag attatgaggc tcttggaaag
    12901 atatcatgtt tcgatgttga agtcaaagga gcttctgatt caatgcaaat gactgtgttt
    12961 ttcaggagat atccttcaca ggatactatt tctattgtta agtctgaacg ttcaggggat
    13021 atttttacaa tttgtattcc atctcaacct cctgctggaa aacttgaata ttttgttgaa
    13081 ttttctgaca gaaatgatca cattacactt ggatatggag aatatcttac aatccgcttt
    13141 aaaaatttag ttcctgtttg ggtgcttttg cctcatgttc tactaatgtt tattgcaatg
    13201 cttttatcaa cttatgttgg gataattgct tttgacaaaa cgcaaaagac tagagtaccg
    13261 gttatgttgt tgatgatatc tttgttcctg ggcggtttac tttttgggcc tatcgttcaa
    13321 aaatatgcct ttggagagtt ttggacaggt tggccgttcg ggcatgacat gacagataat
    13381 aaaacattag ctgccatgtt ggtatggatg gttgccattt tgggcaatag atcaaaaaag
    13441 aaaagatggt ggtatgttgt tgcggcaata tttatgttgc tcatttattc aatacctcac
    13501 agcacatcag gctctgaatt cgattattca aaaaaagaag tagttaccgg tgaaagtgtt
    13561 aatatttgtg agcatcctgt gaattcagat cttcgatgct gatgtttttt ctggttaatg
    13621 ctctccaaac atatacaatg tatgcaatta caaaaggtaa taaaagagta acccaagcca
    13681 tcactttgag ggtaaacaag ctggatgaac tattatagag cgtaagtgaa tcctgaagat
    13741 tgtaagttga aacgaaaaaa gcagtgttgt tgaaagcggc gcaaataagt atactccaaa
    13801 ctgaaaaaac cactccggct gaggctaagt aaaaactata ctttcctttt ccataataat
    13861 tgttacctag accccacaga acaaggacaa ctccaataag aagcattaca gcaggccagc
    13921 caagttcaat catattgaaa aaatatttgt attgaacagg agtaataagt ccggattctg
    13981 gatttacaga gtagcctaca cgtgtaaaaa gagatactaa aaacagaata aaagccacaa
    14041 cgaatccgat tccagtcgct tttacacgag atttgcattt agatttaaga gattcggaat
    14101 caatttgcat aatcacataa agtaatccaa gagttcttgc agctaaaaat acaacaatcc
    14161 ccatgaggaa attaaaaggc actgtaatgg cttcaagacc atgccagctt gatgtccagt
    14221 aagatataga aggttttgcc gtctctgtta tgctcatttt atccatgacg aaagatccac
    14281 ctgtgaaaaa agttgcaaca gcaactccta agagaatagt tcccagagta ccgttaagga
    14341 ataaaaaaac ttcatatgtc tttgcaccaa gaagattacc agccttattt ctatattcat
    14401 atgatactgc ttgtattaca aataaaaaca gtatagttat ccaaagccag taagctcctc
    14461 caaaacttgt tgagtaatag agtgggaagg a
//
LOCUS       DAOTQL010000030        14278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4050_length_14278_cov_5.184138, whole genome shotgun sequence.
ACCESSION   DAOTQL010000030 DAOTQL010000000
VERSION     DAOTQL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14278
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_14278_cov_5.184138"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5293_03125"
     CDS             <1..893
                     /locus_tag="P5293_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48926.1"
                     /translation="ADELARIEKNNSEFMPVYTLLQSFKDSLLKDEAIKKMWEGITYK
                     RVDEFIKYMNDTSSFNKVKDSLHGVWEQQYGQYSHKVDSVLAYWKSYREANDASQYVK
                     IELENIDKEYYPYSNKLNNVSFEIKVTPLRGPIDYLRFSYKVIPRSAIDRAKSDYDIY
                     HSSLYEASRCTITERFSGPLTNYWEANYTNRKILEERNIETFKRDFVVYFEVDELRQG
                     GKRITKSNVDVPASVLNYWKNENDPVMKEKYAKDIVKDILGVDFVSEQEFYNNAFEKY
                     IENKDKEVYELLKLCGYRQF"
     gene            933..1964
                     /locus_tag="P5293_03130"
     CDS             933..1964
                     /locus_tag="P5293_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRR48927.1"
                     /translation="MIINGSGQSGSASTDQMLTLDNYLIPVGVKGAKIADVIVPESCN
                     CDAEVAIVKDTAGIFTIDSKGGISLKKSVTLKTDSGPFVYGITIKAGCLQKEFELVKD
                     QFIKNKVIAHRGAWKNHEASQNSRNSLKYAIEIGCEASELDVWMTSDGAIVLNHDPEI
                     NGKIVEDNTVATMTSVPLKGNDFLPTLEQIIKDVKQQNRTKLVIEIKASLKSQERTNE
                     LTAKVVKLVHDMKAQAWVACYISFNYDACLLVTQIDPTARTAYLGDNKSIEKLKSVNM
                     WGIDFNYKMFEKDPGLIQKAHNAGMTVNVWTVNKKEDLISFLDKGVDFITTNEPEMLL
                     EIIKEKSSK"
     gene            complement(1956..3251)
                     /gene="thrC"
                     /locus_tag="P5293_03135"
     CDS             complement(1956..3251)
                     /gene="thrC"
                     /locus_tag="P5293_03135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRR48928.1"
                     /translation="MKLYSTNNKNNQVSFKEAVIKGIADDGGLYVPVSLPQMQEAFFD
                     RIGILDLQDIAFAVSRRVLKEEIPDYELFKIIRSAFTFTAPLQPLDRDKNILELFHGP
                     TLAFKDFGARFLAETLSFFNQDEDKKLVILVATSGDTGSAVANGFYNVSGIDVVLLYP
                     SGKVSKIQEQQLTTLGGNVTALEVLGSFDDCQRMVKEAFNDKELRSKVKLTSANSINF
                     ARLFPQSFYYHYAYSQLERRDRAVIFSVPSGNLGNLTAGLIAREMGLPVKKFIAALNS
                     NDVFENYLKSGTFQPKTSVQTISNAMDVGNPSNFYRIYSMFDGNLNSMREVIFSRSFS
                     DNQTRQAIGEIYNKYGYLADPHGAVGYLALKEYLEEYPGSYNSIILETAHPAKFKDIV
                     EENSGTSVEIPESLVKCLEKKKKSIIISNNLDALKSILL"
     gene            complement(3256..4194)
                     /locus_tag="P5293_03140"
     CDS             complement(3256..4194)
                     /locus_tag="P5293_03140"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRR48929.1"
                     /translation="MNKCIRVFAPATVANMACGFDVMGFAINKPGDELIITTSNEPGV
                     KITKITGDEGKLPLEPALNTAGKPILSMVKKFRIKCGVEIEINKNMPLGSGLGSSAAS
                     AVAAVFALNALFELKLSKDELLNFALEGEQLSSGTVHADNVAPCLYGGIVLIRSNKPL
                     DIIALKVPDKLYCTIIHPQIVIPTKDSRRILPAEIPLSSAVTQWGNIAALVAGIMKSD
                     YDLIGRSLQDVVIEPVRSQLIPGFSEMKKAALDNGALGCSISGSGPSLFALSKGIKTA
                     SRVGKAMQKCLIKYNLQSELYVSKVNKNGPEILKDR"
     gene            complement(4215..5267)
                     /gene="asd"
                     /locus_tag="P5293_03145"
     CDS             complement(4215..5267)
                     /gene="asd"
                     /locus_tag="P5293_03145"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRR48930.1"
                     /translation="MEEREKLKVAVLGATGSVGQKFIELLQNHPWFQLHEVAASERSA
                     GKKYKDAVNWVQATPLPESMANMIVKECSPNLESKILFSGLDSSVAGEIEEEFASQGY
                     YVISNARNHRFDKDVPLMIPEVNPEHMELVRNQNRSGFIVTNPNCATIGLVMALKPLQ
                     DKFGIEAVNVVTMQAISGAGYPGVASLDILDNVIPFIGGEEEKVESEPLKILGKIVNG
                     AIEEINFKISAQCNRVAVIDGHTEAVQIKLKKRTSVEEIKKAWIEFRSVPQELNLPSA
                     PLQPIHYFDNDKYPQPKLHRNIDKGMAVSIGRLRECPLFDFKFVITSHNTVRGAAGGT
                     ILIAEMMYKNGYFNNL"
     gene            complement(5299..6699)
                     /locus_tag="P5293_03150"
     CDS             complement(5299..6699)
                     /locus_tag="P5293_03150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRR48931.1"
                     /translation="MKVLKFGGSSVSCPSRIREVIQIIINCKRDGSAITVVFSAFQGV
                     TDMLIEMANRASSGDNSYLSVFEELRDRHLNTIRELIQIRNQSHILTNTMMSLKELSD
                     ILHGVVLIKELSAKTLDLVMSYGERLSAYIISEAIKDVGIDAEYIDSRKLVICDDNYG
                     YGRVNYELTYNNIREYYSSHSKLQVVTGFIASTKKGNTVTLGRGGSDYTASLLGAALE
                     AEEIEIWTDVDGVMTADPKKVKGAFPIPFLTYDEAMEMSHFGAKVIYPPTMQPAMDKS
                     IPIRILNTLNPSFKGTLIGRKNDGERYEIKGISSIDNISILLIQGSGMIGVAGIAKRV
                     FGALADANVNVILISQASSEHSICIGVMPDSARQAKRSIEKEFAYEIQAKIVNEVSVT
                     NDLSIVTVVGAQMRHTPGNAGKFFQSLGNIGVNIVAIAQGSSELDISAVIQRNNLEVA
                     LNAIHNTYFKKSKKAS"
     gene            complement(6942..7256)
                     /locus_tag="P5293_03155"
     CDS             complement(6942..7256)
                     /locus_tag="P5293_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRR48932.1"
                     /translation="MLMTTTSTIQGYEIDQYLGLVSGETIIGANIVKDFFAGIRDIVG
                     GRSSSYERVLKQAKEEALNEMCSQAIALGANAVIAIDLDYETVGSSMLMVTAAGTAVK
                     IR"
     gene            complement(7299..8534)
                     /gene="pepT"
                     /locus_tag="P5293_03160"
     CDS             complement(7299..8534)
                     /gene="pepT"
                     /locus_tag="P5293_03160"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRR48933.1"
                     /translation="MESVIDKFLRYISVETTSDPDSETQPSTPSQVEFMKILVKELEQ
                     MGVKDVSLDEFGYVMASIPSNLNNGETAPKLGFIAHVDTSPDAPGKDIKPQFFHNYDG
                     GEIVINKEKNLVLSPIEFPELKKYIGQTIITSDGNTLLGADDKAGVAEIMSAVEYIFT
                     HPDFKHGEIKIAFTPDEEIGRGVDKFDVTRFGAEFAYTLDGGEIGEMEYENFNAASAK
                     IFIQGRNIHPGYAKNKMINSILLATEFNSLLPVEQRPELTEGYEGFIHIVHMSGTVEE
                     SMIQYIIRDHDRAKFEEKKKLVEDCTDFMNCKYKDNRFRLELKDQYYNMKEKVEPHYH
                     IIEKAVEAMKKAGITAQVQPIRGGTDGARLSYMGLPCPNIFAGGHNFHGKLEYVPAES
                     MEKAVEVIINLISLYIQHE"
     gene            complement(8561..9364)
                     /gene="zupT"
                     /locus_tag="P5293_03165"
     CDS             complement(8561..9364)
                     /gene="zupT"
                     /locus_tag="P5293_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRR48934.1"
                     /translation="MDSNLIYAFILTLLAGLATSIGSGIVLFSKKFSSKFLAASLGFS
                     AGVMIFISLVELLNDSRTRLGEIYGENSGFLYALIAFFGGMALIAIIDNLVPSYENPH
                     EVGDLSINENGAAKSDSPLMRVGIMSALVIAIHNFPEGMATFVSAINDPGLGLSIAVA
                     IGIHNIPEGIAVAVPIYYATKSKTKAFTNATLSGLAEPVGAVIGYFLLSFLFHDNFMG
                     IIFAAIAGIMVYISLDELLPTAEKYGEHHIAILGVVAGMFVMGISLVLI"
     gene            complement(9368..11368)
                     /locus_tag="P5293_03170"
     CDS             complement(9368..11368)
                     /locus_tag="P5293_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRR48935.1"
                     /translation="MQKEENKQVGLPENAYRELKEGEEYKPIMSPDKVYPEVSVWSVS
                     IGLLMTVIFSAAAAYLGLKVGQVFEAAIPIAIIAVGLSSAFKRKNALGENVIIQSIGA
                     CSGAIVAGAIFTLPALYILQAKYPELTVDFIKVFLSSLLGGVLGILFLIPFRKYFVSD
                     MHGKYPFPEATATTQVLVSGESGGKGAKLLLISGLIGGIYDFIVSTFGWWSEVFTTRV
                     LPFGETIAEKVKLVFKVNVGAAVLGLGYIIGLKYAFIICCGSFLVWFVIIPLMNLFFG
                     GSIVDLMNSGITEVVGSMSPETIFKTYARHIGIGGIATAGIIGIINSAGIIKSAFGLA
                     VKEIKGSGSGKNNVKRTQKDLPMKIIAIGILVTLAITFIFFYFGVVSNLTHAIIALLV
                     VGIIAFLFTTVAANAIAIVGTNPVSGMTLMTLILASVILVACGLSGTAGMTAALVIGG
                     VVCTALAVSGAFITDLKIGYWLGSTPIKQESWKFLGTLVAAATVGGVMIILNKTYGFT
                     GDSALVAPQANAMAAVIEPLMSGGGAPWLLYGIGALIAIVLTLFKIPALPFSLGMFIP
                     IELNIPLLVGGAVAWFVSTRSKDAALNNARKERGTLLASGFIAGGALMGVVSAIIRFG
                     GIDLTNRQWMESKPAAFLALVMYVLIIVYLALDSMRAKVDDK"
     gene            11607..12221
                     /locus_tag="P5293_03175"
     CDS             11607..12221
                     /locus_tag="P5293_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR48936.1"
                     /translation="MDVDICVANASHVKYAERICELMYISSQERGTGIAKRSPDYIAG
                     KMIAGKAVIALAGEEFAGFAYIETWSHSKFVANSGLIVAHQFRKSGLARRIKAKVFEL
                     SRTLYPDAKIFSITTGLAVMKLNTELGFKPVTFSELTDDEEFWKGCEGCVNFDVLQRN
                     NRKMCLCTGLLYDPEEHKEYKKQNIKPLPLIVSKISSILTFKKL"
     gene            12227..13417
                     /gene="argG"
                     /locus_tag="P5293_03180"
     CDS             12227..13417
                     /gene="argG"
                     /locus_tag="P5293_03180"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRR48937.1"
                     /translation="MKSKVVLAFSGGLDTSFCIPYLKRERGLEVHTVMVNTGGFSPEE
                     EKKIEERAIALGAASHRCVNAVNEYYSKGVKYLLFGNILKNATYPLSVSSERAFQALE
                     VLKVVNEVGAEFVAHGSTGAGNDQVRFDLVFEVMAPEVKIIAPIRELSISRKEEIEYL
                     NKCGFDFSWEKAKYSINQGIWGTSIGGVETLKSSGILPEEAYPSQVTKEGSERIKITF
                     EKGEPCALNGKKMDPVSLIHKLNSIASPYGVGRDIHVGDTIIGIKGRVAFEAAAPLIL
                     IKSHHLLEKHTLTKGQLYWKDQLAGWYGQLMHEAQYLDPVMRNIELFLEDTQKNVCGT
                     VEVELRPYHFAVLACESKYDLMNSRFGEYGEANRSFTGQDVIGFTKVLANPLKIYYSV
                     HEED"
     gene            13404..>14278
                     /gene="argC"
                     /locus_tag="P5293_03185"
     CDS             13404..>14278
                     /gene="argC"
                     /locus_tag="P5293_03185"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRR48938.1"
                     /translation="MKKIKAAIAGGTGYTAGELIRILIRHPYVEIISVLSSSTPGAKI
                     TSFHRDLLGETDMCFVDKVEFAKALSGTDVVFLCLGHGLSREFLDEYNLGKRCKVIDL
                     GNDFRIEGEYGNLHFVYGLCDLFREKIRNASYIANPGCFATAILLALSPLAIVEELTD
                     EIHIHAITGSTGAGRKLSESSNFSYRDNNISVYKPFTHQHIPEIRKSLAELSGSDIPQ
                     INFVPLRGDFTRGIFASIYTRASERFDLSEMIEHFNSVYKNSPYVIISDDSISLKEVA
                     NTNKALIHVEKHNGY"
BASE COUNT         4356 a         2793 c         2802 g         4327 t
ORIGIN      
        1 ccgctgacga gcttgccagg atagaaaaga ataattctga gtttatgcct gtatatactc
       61 ttttacagtc ttttaaggat tccctgttaa aggatgaagc tataaaaaaa atgtgggaag
      121 gaattactta caaaagagtt gatgaattta tcaagtatat gaacgatacc agctctttta
      181 acaaagtaaa agacagtctt cacggtgtgt gggaacagca gtatggccag tattcccaca
      241 aagttgactc agttttggcc tactggaaaa gctatagaga ggctaatgat gccagccagt
      301 atgtaaagat agaattggaa aatatagaca aagaatatta tccttactct aacaagctta
      361 ataatgttag ctttgagata aaagtaaccc cactgcgtgg ccctatagat tacctgagat
      421 tcagttacaa agtgattcca aggtctgcaa tagacagagc caagtctgat tatgatattt
      481 accattcttc tctttatgaa gcgtcccggt gtactatcac agaacgtttt tcaggacctt
      541 tgacaaatta ctgggaggct aattacacaa acagaaagat tcttgaggag aggaatattg
      601 agactttcaa gagagatttt gtagtttatt ttgaggttga cgaacttaga cagggaggta
      661 aaaggatcac aaaaagcaat gttgatgttc cggcgtcagt gttgaactat tggaagaacg
      721 aaaatgatcc ggttatgaaa gaaaaatatg ccaaagatat agttaaggat attttagggg
      781 ttgattttgt ttctgagcag gaattttaca acaacgcatt tgaaaaatat atagaaaaca
      841 aagataaaga ggtttatgaa cttcttaaac tttgcggtta tcgtcaattt taaatattta
      901 ttatgaaatt ctttttatta atttgttgtt cgatgattat caatgggtca ggccagtcag
      961 gatctgcaag cacagatcaa atgctaactc ttgacaatta tttgattccg gtaggtgtca
     1021 aaggtgcaaa aattgccgac gtaattgtgc ctgaaagctg taattgtgat gcagaagttg
     1081 ctatcgtgaa ggatacggcc ggtattttca ctattgacag caaaggaggt atatctttga
     1141 agaagagtgt aactttgaaa actgattcag gtccttttgt gtatggtatt acaattaaag
     1201 caggttgcct gcaaaaggag tttgaacttg ttaaggatca gtttataaaa aataaggtaa
     1261 ttgctcacag aggagcttgg aaaaatcatg aggcaagtca gaattcaaga aattcactca
     1321 aatatgctat tgagattggt tgtgaagctt ccgaactgga tgtgtggatg acctctgacg
     1381 gagcaattgt ccttaaccac gatcccgaaa ttaatggcaa gatcgtagaa gacaacacag
     1441 ttgctactat gacttcagtg cctctcaaag gaaatgattt tctgcctact cttgagcaga
     1501 ttataaaaga tgtcaagcaa caaaacagga caaaactggt gattgaaatc aaagcatccc
     1561 taaagagtca ggaaagaaca aatgaattga cagcaaaagt tgtaaagctt gtacatgata
     1621 tgaaggctca agcctgggtt gcttgctata tttccttcaa ctatgatgct tgtctcctgg
     1681 ttacacagat tgaccccaca gcgagaactg catacttggg agacaataag tcaatagaga
     1741 agctcaaatc cgtgaacatg tggggtatag acttcaatta caaaatgttt gaaaaagatc
     1801 caggtttaat tcagaaagca cataatgcag gaatgacagt aaatgtatgg acagtaaata
     1861 aaaaggaaga tctaatctca tttttggaca aaggggttga ctttattaca accaatgaac
     1921 ctgagatgct tcttgagatt ataaaagaga aaagttcaaa gtaaaatact tttcagagca
     1981 tccagattat tggaaataat tatgctcttt ttcttcttct ccagacattt cactagagat
     2041 tcggggatct caacagaggt tcccgaattt tcttctacta tatccttgaa ttttgcaggg
     2101 tgagctgtct cgagtatgat tgaattgtaa gatccgggat actcttcaag atattctttg
     2161 agagcgagat atcccacagc tccgtgagga tctgcaagat atccgtattt gttatatatc
     2221 tctcctattg cctgacgcgt ctggttatct gaaaatgacc gactgaaaat aacttctctc
     2281 atgctgttaa gatttccatc aaacatagag tagatgcggt aaaaattgct tggatttcct
     2341 acatccatag catttgatat ggtttgaacg gaggttttag gttgaaatgt gcctgactta
     2401 agataatttt caaacacatc attagagttt aaagctgcga taaacttttt gacaggcaac
     2461 cccatttctc ttgcaatcaa tccggcagta agatttccca gattaccgct tggtactgag
     2521 aaaatgaccg ctctgtcacg tctttcaagt tgagagtagg catagtgata ataaaaggat
     2581 tgagggaaca gtctggcaaa attaatggaa ttggcagagg taagtttaac ctttgatctt
     2641 aattctttgt cattaaaggc ttccttgacc attctttgac aatcatcaaa actgcctagt
     2701 acctcaagag cagtgacatt gcctccgaga gtggtaagtt gctgttcctg tatcttgcta
     2761 actttgcctg atggatacaa tagtactacg tcgatcccgg aaacattgta aaatccattt
     2821 gcaactgcac ttcctgtatc gccggatgtt gcaaccagaa taaccaattt cttatcttca
     2881 tcttgattga agaaggagag agtttcagcc aaaaaacgtg ctccaaaatc cttaaaggca
     2941 agtgtgggtc cgtgaaacag ttcgagaata tttttgtctc tatcaagtgg ttgaagaggc
     3001 gctgtaaagg taaatgcgct tctgattatt ttgaaaagct cgtaatctgg tatttcttct
     3061 tttaagactc ttcttgatac ggcaaatgca atgtcttgca agtcgaggat ccctattctg
     3121 tcaaaaaagg cttcttgcat ttgtggcagt gaaacaggaa cgtataatcc gccatcatcg
     3181 gctattcctt taattaccgc ttccttgaat gagacttgat tgtttttgtt gttagtgctg
     3241 tagagtttca ttgatctatc tgtctttcaa aatttcagga ccatttttgt taacctttga
     3301 cacatatagt tcagattgaa gattgtactt aattagacac ttttgcattg cttttccaac
     3361 ccgagaagct gtttttatcc cttttgaaag agcaaacagt gacggtccgg agccggatat
     3421 gctgcaaccg agtgcaccat tgtccaaagc tgcttttttc atttctgaaa aaccagggat
     3481 aagttgagac ctaacaggtt ctataacaac gtcttgcagt gagcgtccaa tgagatcata
     3541 atcacttttc ataattccgg ctacgagagc cgctatgttt ccccactgag ttacggcact
     3601 tgaaagcggt atctctgcag gcaatatcct tctagaatct tttgtgggga tcacgatttg
     3661 tgggtggatt atagtacagt aaagtttgtc gggcactttc agtgctataa tgtcaagagg
     3721 tttgttggag cgaatcaaaa cgattccacc ataaagacat ggagcaacat tatcagcatg
     3781 aacagttcca cttgatagtt gttcaccttc gagagcaaag ttcagaagct catctttaga
     3841 cagtttgagt tcgaagagag cattaagagc aaaaactgcc gcaacagcac ttgcggcgct
     3901 tgaaccaagt ccgctgccaa gaggcatatt cttgttaatc tcaatttcaa cgccacattt
     3961 tatcctgaac ttttttacca ttgaaagtat cggtttgccg gccgtgttaa gggcaggctc
     4021 aagaggcaat tttccttcgt ctcctgtgat ttttgtgatt ttaactccag gttcatttga
     4081 ggttgttatg ataagttcat ctcccggctt attaattgca aaccccatca catcaaatcc
     4141 acatgccata tttgccactg ttgcgggggc aaataccctt atacacttgt tcattattta
     4201 taataattaa aatctcagag attattaaaa tacccatttt tatacatcat ttcggctatc
     4261 agaatagtgc ctccggcagc gcccctgaca gtattgtgtg aggtaatgac aaacttgaag
     4321 tcaaataacg gacactctcg taaacgtcct attgacacag ccattccctt atctatattt
     4381 ctgtggagtt ttggttgggg atatttatca ttgtcgaaat aatgaatggg ttgaagcggg
     4441 gcagatggta aattaagttc ttgtggaaca gatctaaatt ctatccatgc cttttttatc
     4501 tcctcgacag aagttctttt tttaagttta atctgcacag cctcagtatg tccatcgatt
     4561 acggcgactc tattacactg ggcacttatt ttgaaattta tctcttcaat ggctccgttg
     4621 acaattttcc ccagtatttt caatggctca ctctctactt tttcctcttc tcctccgata
     4681 aaaggtatta cattgtcgag aatgtcgagc gaggccactc ccgggtagcc tgctcccgat
     4741 attgcctgca tggtgactac atttacggct tcaatcccaa acttgtcctg aagaggttta
     4801 agtgccatta caagaccaat tgttgcgcag ttagggttag taacaataaa gcctgagcga
     4861 ttctgatttc tgacaagctc catatgttcc ggattaactt ctggaatcat aagtggcaca
     4921 tctttgtcga aacgatggtt gcgagcattg gaaatgacat aataaccttg tgaggcaaac
     4981 tcttcttcaa tctcacctgc cacagatgaa tcaagacctg agaatagaat tttgctttcc
     5041 aaattgggac tgcattcctt tacaatcata tttgccatac tttctggcag aggagtagcc
     5101 tgtacccaat tgacagcatc tttatatttt tttcctgctg aacgttcaga tgctgctact
     5161 tcgtgcaatt gaaaccaggg gtgattttgc aacaattcaa taaatttttg gcctacgcta
     5221 cctgtagctc ccaatacagc aacttttaat ttctctctct cttccatggg gattagattt
     5281 attttattct aaaaatgatt atgatgcttt tttgcttttt ttaaaatagg tgttgtgaat
     5341 tgcatttaat gcaacttcaa gattatttct ctgtataaca gccgaaatat caagttctga
     5401 agagccctga gctatggcga caatattgac tccgatattg cctagtgact ggaaaaattt
     5461 tcctgcattt cccggagtgt gcctcatctg agctccaact accgtaacaa tagacagatc
     5521 atttgttaca cttacttcgt ttacaatttt tgcctgtatt tcgtaggcaa actctttttc
     5581 aatactccgt ttagcctgtc tggcagaatc tggcatgact cctatgcaaa tagagtgttc
     5641 agaagatgcc tgagagataa ggatcacatt gacatttgca tctgccaaag cgccgaacac
     5701 tctctttgca attcctgcaa cacctatcat tccacttccc tgaatcagaa gaatggatat
     5761 attgtcgatt gatgaaattc ctttaatctc atatcgttcg ccatcgtttt ttcttccaat
     5821 taaagtccct ttgaaagatg ggtttaaggt attcagtatt cttatcggta tgcttttgtc
     5881 catagccggc tgcattgtcg gaggataaat aacctttgct ccaaagtgcg acatttccat
     5941 agcttcatca taagtaagaa agggtatggg gaatgctcct ttaaccttct ttggatcagc
     6001 tgtcattact ccatcgacat ctgtccatat ttctatctct tctgcttcaa gcgcagctcc
     6061 aagaagggaa gcagtataat cagatccacc ccggccaagg gtaacagtat tacctttttt
     6121 tgtggaggca ataaacccgg taacaacctg caattttgaa tgtgaactat aatattctct
     6181 tatattgttg tatgtgagtt cataattaac acgtccgtat ccataattat cgtcacaaat
     6241 caccaatttt ctgctatcta tatactctgc atcaattcct acatccttga tagcttcgct
     6301 aataatataa gcagagagtc tttcaccata actcatcacc agatcaaggg ttttggcaga
     6361 caattccttt atcagtacta ctccatgcaa tatatccgac aactctttca agctcatcat
     6421 agtatttgtc agaatgtgac tttggtttct tatctgtatc aactctctta tggtatttaa
     6481 atgcctgtct cttaattctt caaaaacaga caaataacta ttgtcacctg aagatgcccg
     6541 gtttgccatc tcaattaaca tatccgttac tccctgaaat gccgaaaata caaccgtaat
     6601 agctgaacca tctctcttac aatttataat aatttgaata acctctctta ttcttgaggg
     6661 gcagcttaca gatgatcctc caaattttaa tactttcata gtcgagcaat taacagggtt
     6721 attaataaaa aaccggcctc ctgaatcaag aagccggttt caatttacct acaaactaaa
     6781 aattaccaac ttccatcata caacattgtt gttgtgattg ttgacatgga tgttttacac
     6841 ataatgacag aagttgaatg taaagttgtc attttatctt tcgaattata acttgacaaa
     6901 tgtagtggaa atatttatta atgcaaatat ttaaaaaaaa attatctaat cttaacagca
     6961 gttcctgccg cagtaaccat aagcattgaa gatccaactg tctcgtagtc aaggtctata
     7021 gcaataacag cattggctcc gagtgctata gcctgtgaac acatctcatt aagcgcttcc
     7081 tcttttgcct gtttgagtac tctctcataa gaactagatc tgccacctac aatatctctg
     7141 atcccagcga agaaatcttt gactatattg gcacctataa tagtttcacc tgatactaaa
     7201 ccgaggtact gatcaatttc atacccttga atagtggatg ttgttgtcat taacattgtg
     7261 ttgaatttta gattgttgaa ttaagttgat tattgtattt attcatgttg aatataaagt
     7321 gatataagat tgatgattac ctcaactgcc ttttccatac tttcagccgg cacatattcc
     7381 agtttaccgt ggaaattgtg accacctgca aatatattag gacatggaag tcccatatat
     7441 gacagacgtg ctccatcggt accacccctt attggttgga cttgagcagt aatcccggcc
     7501 tttttcatgg cttcaaccgc tttttcaata atgtggtaat gaggttctac cttttccttc
     7561 atgttgtagt actggtcttt aagttcaagt ctaaagcgat tgtctttgta tttgcaattc
     7621 ataaaatctg tacaatcctc aacaagtttc ttcttttctt caaatttggc tctgtcatga
     7681 tcgcgtatga tgtattggat cattgattct tctactgtac cactcatatg aactatatgt
     7741 atgaatcctt catagccctc tgtcaattca ggtctctgtt ctacaggcag gagagaatta
     7801 aactctgttg caaggaggat agagttaatc atcttatttt tagcataacc cggatggata
     7861 ttccttccct gaataaatat tttggcagat gctgcgttga aattttcata ttccatctct
     7921 ccaatttcac ctccgtccaa agtatatgca aactcagctc cgaaacgggt tacatcaaat
     7981 ttatccactc cgcgtccaat ctcttcgtca ggagtaaatg ctattttgat ctctccatgt
     8041 ttgaaatcag gatgagtaaa gatatattcc accgcagaca ttatctctgc tactcctgcc
     8101 ttatcatctg ctccaagaag agtattgccg tcagaagtta tgatagtctg cccaatatat
     8161 tttttcaatt cggggaattc gatcggagag agaactaaat ttttttcttt gttaattact
     8221 atctcgccac catcgtaatt gtgaaaaaac tgaggtttta tatctttacc tggagcatcc
     8281 ggcgaagtgt ccacatgtgc tataaagccg agtttgggag cagtttcccc attattaaga
     8341 tttgaaggga tggaggccat aacatagccg aattcatcga gtgaaacatc ttttacaccc
     8401 atctgttcaa gctctttgac aagtattttc atgaactcca cctgagaagg tgtgcttggt
     8461 tgtgtttcag aatctggatc tgacgttgtt tcgactgaga tatagcgtaa aaatttgtct
     8521 attactgatt ccataattat tagatctaaa agttaattaa ttaaataagc acaaggctta
     8581 ttcccattac aaacattcct gcaacaactc ccagaatggc aatatggtgt tccccatact
     8641 tttcagcggt aggaagaagt tcatcaagag atatatatac cataattccc gctattgcgg
     8701 cgaaaataat acccataaaa ttatcgtgga agaggaatga tagcaaaaag tatccgatta
     8761 cagctccaac aggttctgca aggcctgata atgtggcgtt tgtaaaagcc ttggtcttac
     8821 tttttgttgc atagtaaata ggtactgcta cggcaatccc ttcaggtatg ttgtgaattc
     8881 cgatagcaac agcaatactt agaccaagtc ccgggtcatt tattgcactt acgaatgttg
     8941 ccatgccttc cgggaagttg tgaattgcaa tcacaagggc agacattatt cctactctca
     9001 ttagaggaga gtcgctcttg gcagctccgt tttcattaat tgacaaatcc ccgacttcgt
     9061 gagggttttc ataggacggt actaaattgt cgataatagc gatgagtgcc atacccccaa
     9121 aaaatgcaat caatgcatac aaaaagccgg aattttcgcc ataaatctct ccaagtcgtg
     9181 ttcttgagtc gtttaacagc tcaaccagag atataaagat cattactccc gcagaaaaac
     9241 caagcgatgc ggctagaaat tttgagctga attttttgga aaaaagaact atcccactac
     9301 ctatagaggt agcgagaccg gcaagaaggg tcagtataaa tgcataaata agattgctgt
     9361 ccatttttta tttatcatca acttttgctc tcatgctgtc caatgccaaa tatacaatta
     9421 tgaggacata cattacaagt gcgaggaaag cggcgggttt agattccatc cattgacggt
     9481 ttgtaagatc aatcccgcca aatcgtatta tagcacttac aacacccatt aaggctccac
     9541 ctgcaataaa accggaagca agaagagtcc ctctctcttt tcgcgcatta ttaagtgctg
     9601 catccttact gcgtgtggaa acaaaccaag caacagcccc tccaaccagg agagggatat
     9661 tgagttcgat aggaataaac attccgagag aaaatggaag agccggaatc ttgaagagag
     9721 taagaacaat agctatcaac gcgccaatac catacaagag ccatggagca cctccacccg
     9781 acattaaagg ttcaatgact gcagccatgg catttgcctg cggtgctaca agagcactat
     9841 cgcctgtgaa accataggtt ttgttgagga taatcataac accgccaact gtggctgctg
     9901 caacgagtgt accgagaaat ttccaggact cctgtttaat aggtgtagaa ccgagccaat
     9961 aaccaatctt gaggtcagta ataaatgctc cggaaacagc gagtgcagta catacaacac
    10021 ctccaatcac aagggcagca gtcatacctg ctgtacctga aagaccgcag gcaaccaata
    10081 ttacagatgc aagaataaga gtcatgagtg tcatgccact tactggattg gttccaacaa
    10141 tagcaatagc atttgcggcg acagtagtaa acaaaaaagc aataatgccg acaaccagca
    10201 acgctattat tgcatgtgta agatttgaaa ccactccaaa atagaaaaat ataaatgtta
    10261 ttgcaagagt caccaaaatg ccaattgcaa taattttcat tggaagatct ttctgagtcc
    10321 tcttaacatt gttctttccc gaaccggaac ctttgatttc ctttactgca aggccaaagg
    10381 ccgatttaat gatgcctgcg gaattaataa tgcctataat tccggcagtt gcgatacctc
    10441 caataccgat gtgtctcgcg taggttttaa atattgtctc aggcgacatg cttcccacaa
    10501 cctctgtaat gccactattc atcaaatcaa caatacttcc tccaaaaaag aggttcatga
    10561 gaggtattat gacaaaccag accagaaaag aaccacaaca tataataaaa gcatacttca
    10621 gtcctattat ataccctaaa ccaaggacag cagcaccaac attgacctta aaaacaagtt
    10681 ttaccttttc agcaattgtc tctccaaaag ggagaactct tgtagtaaac acctctgacc
    10741 accaaccaaa agtagataca ataaaatcgt aaataccacc aatcaagcca cttataagaa
    10801 gtaattttgc tccttttcct ccactttcgc cgctgactag cacctgagtg gttgctgtag
    10861 cttctgggaa tggatacttt ccgtgcatgt ccgaaacaaa atacttgcgg aaagggatca
    10921 gaaacaaaat tccgagaact ccgccgagca gagaactaag aaaaactttt ataaaatcaa
    10981 cagtaagctc cggatattta gcttggagta tatatagagc aggaagagta aatatcgctc
    11041 cggccacaat agcaccagag catgcaccga ttgactgaat aatcacattt tctccaagtg
    11101 catttttcct tttgaaagca cttgaaagtc cgacagcaat aattgcaata ggaatagctg
    11161 cttcaaatac ctgtcctact ttcaaaccaa gataggctgc tgctgcagaa aaaatgactg
    11221 tcataagcaa accaattgac acagaccaaa cacttacctc tggataaact ttgtcaggtg
    11281 acataattgg tttatattcc tctccttctt taagttctct ataggcgttt tccggtaagc
    11341 ctacctgttt attctcttct ttttgcataa ttttaatgat tagtgaccaa agatagaaat
    11401 tttataaata attattttat ctttgcaccc acaatgaaaa ccgtttactt caatattgaa
    11461 cttaaaccca ggcccagaca cgtccgacga tgagcctgag gggacggtta taacttaatc
    11521 ccttagggac cacagcgaaa ttctacgcag gtatagtctc ctgcaaatta atcatacata
    11581 ttttaacttt atattgttaa ttactaatgg atgtagacat ctgtgtggct aatgctagcc
    11641 atgtaaagta tgcagaaagg atctgcgaat tgatgtatat ctcttcacag gagaggggta
    11701 caggcattgc caaaagatcc cctgactata ttgccggaaa aatgattgcg ggtaaggccg
    11761 ttatagcgtt agccggagag gaatttgcgg gctttgccta tattgaaaca tggagtcaca
    11821 gcaagtttgt agccaattcg ggtctgatag ttgctcatca gtttagaaag tcaggattgg
    11881 caagaaggat aaaagcaaaa gtttttgagc tttcacgaac actgtatcct gatgccaaga
    11941 ttttcagtat taccacaggt cttgcagtta tgaagcttaa taccgaattg gggtttaaac
    12001 cagttacatt ttcagagttg actgatgatg aagagttttg gaaaggttgc gagggatgtg
    12061 ttaactttga tgttttacag agaaataaca gaaaaatgtg cctttgcaca ggattacttt
    12121 atgatcctga agaacacaaa gaatataaaa agcagaatat aaaacctctt ccgcttatcg
    12181 tttcaaagat aagttcaata ttaaccttta aaaaattgta aaagagatga aaagtaaagt
    12241 tgttcttgca ttcagcggcg gccttgatac atctttctgt atcccttatt taaaaaggga
    12301 gaggggcctt gaggtgcata ctgttatggt aaatacggga gggttttctc ctgaagagga
    12361 aaagaaaatt gaggaaagag ccattgcttt aggggctgct tctcacagat gtgtaaatgc
    12421 tgtaaatgag tactattcca aaggtgtaaa atatcttctt tttggcaata ttctcaaaaa
    12481 tgctacttat cctctttctg ttagctctga aagagctttt caggctcttg aagtattgaa
    12541 ggtagtaaat gaagttggag cggaatttgt ggcacacggc agcacgggag ctggaaacga
    12601 tcaggtgaga tttgatcttg tttttgaagt tatggcacct gaagttaaga ttattgctcc
    12661 cataagggaa ctttccatca gcagaaagga agagatagag tatctcaaca aatgtggatt
    12721 tgacttttcg tgggaaaagg ctaagtattc tataaatcag ggtatttggg ggacaagtat
    12781 tggtggagta gagactctta aatcttccgg tattttacct gaagaagctt acccgtctca
    12841 agtaaccaaa gagggaagcg aaagaataaa aataacattc gaaaaaggag aaccttgcgc
    12901 actcaatggg aaaaaaatgg atccagtaag tctgattcac aagttgaatt ctatagcttc
    12961 tccttatggt gtgggcagag atattcatgt cggagataca attattggca ttaaaggcag
    13021 ggttgcattt gaggctgctg caccattaat tctcattaag tcgcatcacc tgcttgaaaa
    13081 acacacactt acaaagggac agctatactg gaaagatcaa ctggccggat ggtatggaca
    13141 gttaatgcat gaggcacagt atttggaccc tgtaatgaga aatatcgaac tttttctcga
    13201 ggatacccaa aagaatgttt gtggcaccgt tgaagtggaa ctcagaccat atcattttgc
    13261 agtactcgct tgtgaaagta aatatgatct catgaattcc agattcggag agtacggaga
    13321 ggcaaacaga tcatttacag ggcaggatgt aattggcttc actaaagttt tggctaatcc
    13381 gttgaaaata tattattcgg ttcatgaaga agattaaggc agcaatagca ggaggcacag
    13441 gatacacggc tggggaattg atcagaattt taatacgtca tccttatgta gagataattt
    13501 ctgttcttag cagcagcact cccggggcga agattacatc atttcacaga gatctgcttg
    13561 gagaaacaga tatgtgtttt gtggacaagg tagaatttgc caaggctctt tccgggacag
    13621 atgtggtatt tctttgtctc ggtcacgggc tgtcgaggga atttctcgat gagtataatc
    13681 ttggtaaaag atgtaaagtc atagatcttg gtaatgattt cagaattgaa ggagaatatg
    13741 gaaatttaca ttttgtctat ggtctatgtg atctttttcg cgagaagata agaaacgcat
    13801 cttatattgc aaacccgggt tgttttgcta cagccattct tcttgcactt tctcctttgg
    13861 ctattgttga agaactgact gatgaaattc atatacatgc tattacaggt tcaacgggtg
    13921 ccggaaggaa actttcggag agttctaatt ttagttatag agacaataat atttctgtat
    13981 acaaaccttt tacccatcag cacattcctg aaataaggaa gagccttgcc gagctttcgg
    14041 gaagcgatat tccgcaaatc aattttgtgc ctttgagagg ggattttacc aggggaattt
    14101 ttgcgagtat ctatacacga gcctcagaga ggtttgattt aagtgaaatg attgaacatt
    14161 tcaactctgt ttacaaaaac tccccatatg tgataatctc cgatgatagt atttcactta
    14221 aagaagtagc taatacaaac aaggccttaa ttcatgtgga gaagcataat ggttactg
//
LOCUS       DAOTQL010000031        14113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4103_length_14113_cov_5.438256, whole genome shotgun sequence.
ACCESSION   DAOTQL010000031 DAOTQL010000000
VERSION     DAOTQL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4103_length_14113_cov_5.438256"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="P5293_03190"
     CDS             <1..379
                     /locus_tag="P5293_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48939.1"
                     /translation="WVGFYIVRPDNSQFGRSLVLGPFQGPIACTRIAYGKGVCGTSWK
                     EKRSLIVADVDKFPGHIACSSFSRSEIVIPIFGEFESKDGENCKEPGEVKALLDIDSK
                     FLDSFDDIDREYLEILVKNIKFT"
     gene            379..2961
                     /locus_tag="P5293_03195"
     CDS             379..2961
                     /locus_tag="P5293_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRR48940.1"
                     /translation="MNRWRLTNLCVFTLFYCAFFFIAGCSVKVPKEAVVGVSEELAEM
                     RTVNISNVRYNLLFEIPKERANNVEGILSLTFDVKKRTHIVLDFKQGDSSVISVTVNG
                     KPGKYVFENEHIILSSRQILKGENQVNIKFHAGDQSLNRREDLLYTLLVPDRARTLFP
                     CFDQPDIKSKYSLSLEIPAGWEAVANGQTVQRDTIKQNDSQYIVFSETEPIPTYLFSF
                     VVGKMNKITKAQDGRKVSIYHRESAKDKIAQCDEIFSQIFSALRWMEDYTNVKYPFTK
                     YDIIIIPGFQYGGMEHMGATLYSDKTMFLSDNPTIAEKLARAELVAHETAHMWFGDYV
                     TMKWFDEVWTKEVFANWFAKRMVAPLFPEVNHQLNFLNSYFPAAYAEDRTAGATPVRQ
                     ELDNLNNAGLVYGNIIYDKAPIVMDKLVRMVGEDNFKKGIREYLENYSYGNATWNDLI
                     NILDKFFDKDLKQWSHVWINEAGMPRITATISGDSTVWKTDDPMGRGLFWEQPLSYKI
                     ENSYLLPNVDGKGYGYFMLDRETSEYLLGKFADYRFSNTYKDTSVVYNDLARCSLLIT
                     LYENLLHNNLDPVSFCNALTKYVPYEDNPLILSRALSYLSDVFVSYIYENGSSFNDGG
                     MPAKIEQTLLNMFENRERMEDRLLAFRTFYGICSSREGVNLLYSIWKDPGSFKAVKLG
                     ERELTNLSYAVLLRLPEIYNEVKTIQVQRITNPDRKAEFNYILPSLSGSKEVRDSVFQ
                     SLLLEKNRQVEPWTQMSLGFLNHYSRQESSLEYIKPGLDILEEIQRSGDIFFPTGWLR
                     ALLSGHHSERAAGIVQEFLDTHKNYPPMLKSKILQQSDHLFREKRSSTFVGGEEVDHL
                     QNKK"
     gene            2961..3866
                     /locus_tag="P5293_03200"
     CDS             2961..3866
                     /locus_tag="P5293_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRR48941.1"
                     /translation="MISRQRQALLMGGMVVLCWATVATAFKIALAEMSYLQLLLISSI
                     IALVICFVELLRSGKFGEVLQLLCRKNARKVGVLMLQALLAPFLYYLVLFKAYSLLPA
                     QIAQPLNYSWQVVLILMMSLFYRQKLSLRQLTGVLICFTGVISLSLNGEADISGKLSV
                     LGIALALGSSLIWSSYWILKLKNEMDPVVELFFNFLFGSVYLIIISFFTPMEWPSLRG
                     FGAAVYVGCFEMGFTFILWAKALRMAENKISLTQLTYLSPLLSLILIHFILGENITYL
                     TVLGLALVIGGIYITNKRVSAPGES"
     gene            3956..4026
                     /locus_tag="P5293_03205"
     tRNA            3956..4026
                     /locus_tag="P5293_03205"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3988..3990,aa:Cys,seq:gca)
     gene            complement(4049..4411)
                     /locus_tag="P5293_03210"
     CDS             complement(4049..4411)
                     /locus_tag="P5293_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HRR48942.1"
                     /translation="MYFEDFDNTETQERNGDDVYSKPVRAGKRTYFFDVKATKNNDYY
                     LTITESKRRLDRDGRFVYDKHKIFLYKEDFDKFSQGLEEIIQFIKEKAPSVEGKNEKN
                     SSGDAQITPFSDVNFEEL"
     gene            4501..5448
                     /locus_tag="P5293_03215"
     CDS             4501..5448
                     /locus_tag="P5293_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination protein NusB"
                     /protein_id="HRR48943.1"
                     /translation="MINRRLIRVKVFKVLFSRMSSGSESMDGAEKELLMSCEKTLELY
                     YFLLTLPTALKRLSEIKIESGLKKFHPTEEEANPNRRFIENAVIAKLENCRELTDFCE
                     KKGLLWNDYDSVVKKIHSSLMEREYYRSYMNAAENTFEDDLKVVMNIFEQELEDCEDL
                     SNSLEDASLYWVDDLAYVINIILKRLSHLKTNSKISFPKVFIKEDDREFAIRLLGKAM
                     LNYDEYVELMSKYVLNWELDRIASTDMNLIVLGIAEAIAFPNIPLKVTINEYVELSKF
                     YSTQNSKIFVNGILDKVLLGLQKEGIIQKSGRGLVGSID"
     gene            5481..5807
                     /gene="yajC"
                     /locus_tag="P5293_03220"
     CDS             5481..5807
                     /gene="yajC"
                     /locus_tag="P5293_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRR48944.1"
                     /translation="MNFLVFLQSGASQQGGGGTWSFLIMMLLIFAVMYFFMIRPQQKR
                     QKELAKFRSNLAKGDKIITMGGIYGIIVDVKDQYVIVEVDNNVKLKVDKGSIVKDSSD
                     IQAAGK"
     gene            5819..6796
                     /locus_tag="P5293_03225"
     CDS             5819..6796
                     /locus_tag="P5293_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48945.1"
                     /translation="MLKRRKIGISYERGRQVLLFLFCLFLAFVLWAIRNLSDYYSVNL
                     NYSVHVVTQKRGHSAEAFSDNKLSVTGRASGFYILQNRLGIQGNLITLSVPGYMLKPL
                     SGTEDKFFFVVSEVRDIITDALSGHVEIESFSSDTLYLTLPRQSHRNIPVAVDYKASF
                     KDQHMPVGKIKISPSTVTVYGDISLLQNIDSVRTKHLTFERLSSTRSGIVELLPVSGL
                     RFETKEIYYTLEVVRYVENSTTIPVESVNVPEGKYVKFFPNTVRVTYRIPFSNKQNRE
                     NTIFVATIDYKEITASISNTVEPMVSGLPENVLSVELDPKFIKCKIIEQ"
     gene            6808..7404
                     /gene="coaE"
                     /locus_tag="P5293_03230"
     CDS             6808..7404
                     /gene="coaE"
                     /locus_tag="P5293_03230"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRR48946.1"
                     /translation="MFCFACTGGIGAGKSYVIRIFSALGVPAYVADDRVKYLYDVDRN
                     LIDKLVDLLGKEIVTDGVLQRKVMAKIIFSDRNLLEKVNNIVHPRLLEDFYEWREQKR
                     SEGIEIIIYESAIFFKTPIFWHLADKVIVVFAPENVRIMRVMKRDGLSEEQVRERMRH
                     QMDYDSMIARADFVIYTDGKRAVLPQVLSILESVKYKC"
     gene            7385..8152
                     /locus_tag="P5293_03235"
     CDS             7385..8152
                     /locus_tag="P5293_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRR48947.1"
                     /translation="MSSISVEMGYGKWITGALGWALGGPIGGIIGFAIGSIFEKGKQI
                     EQYQQQEPSAGRRYSNIEQRNSFLVSLLVLSSAVMKADGRIMQSELEYVKRFIRQNFG
                     EDAEREASLFLRDLLKKDVDINAVGLQIRNYMNYEARIQLLHYLTGIAQADGSVSSSE
                     LDVLRTISAGLGISRAESESIFAMFDNGLEAAYKVLEIDKNATDDEVKKAYRRLATKH
                     HPDKVSNLGPDVQKAAEERFKSISEAYEKIKKDRGIV"
     gene            8164..8580
                     /locus_tag="P5293_03240"
     CDS             8164..8580
                     /locus_tag="P5293_03240"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRR48948.1"
                     /translation="MKTNLKKILSISGQPGLYNYLSNANAGVIVESLLTKKKTICGSS
                     FRLTALSDISIYTSDDELPLHVVFTKMHEKLGDQSAPSGKSSPDELRTFFEEVIPNYD
                     KVRFYPSHMKKVVDWYNVLKDNASLDFELPDEEKQN"
     gene            8561..9889
                     /gene="rimO"
                     /locus_tag="P5293_03245"
     CDS             8561..9889
                     /gene="rimO"
                     /locus_tag="P5293_03245"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRR48949.1"
                     /translation="MKKSKIKLITLGCSKNRVDSEHLLKQVEKSGIEISPESESVEDA
                     RADVLIINTCGFIKDAKVESIQAILEAVEAKKHGFTSKVLVFGCLSQRYREELVRSIP
                     EVDGFYGVFDIEQVVAALGSCWNSNYETQRVLTTPSHYAYLKISEGCDRTCSYCSIPI
                     IKGKHVSVPVEKLVSEAQYLALLGVKELILVAQDTTYYGVDLYGKRCIAELVKRLSDI
                     EGIEWLRLLYAYPAAFPEDLLEVMANNSKVCKYLDIPLQHVSDKVLRNMKRSVNGEQT
                     RRLVEMFRRNVPGIVLRTTMMVGHPGEDNKAFEELLSFVSQARFERLGAFTYSEEEGT
                     WSAHNLRDTVRAEVKEERYKRLMELQSGVSMEYNESRIGTKEKVLVDSVSDRYLIARS
                     RNESPEVDGEILIDRIINSSNLNSKDLIGTFVDIEITDADEYDLYAKLIL"
     gene            9906..10652
                     /gene="fabG"
                     /locus_tag="P5293_03250"
     CDS             9906..10652
                     /gene="fabG"
                     /locus_tag="P5293_03250"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRR48950.1"
                     /translation="MKLLDGKVALITGAARGIGKAIALKFASEGADIIFTDLVIDENA
                     ETTKREIESFGVKVLALASNASSFEEAHKVVDEGVKVFGKIDILVNNAGITKDGLIMR
                     MTEQQWDAVINVNLKSAFNYIHAITPVMMRQKSGSIINMSSVVGVSGNAGQANYAASK
                     AGMIGLAKSVAQELGSRGIRANAVAPGFILTDMTEKLPDAVKKEWAEKIPLKRGGLPE
                     DIANVCTFLASDLSSYVTGQVISVCGGMKM"
     gene            10665..11588
                     /locus_tag="P5293_03255"
     CDS             10665..11588
                     /locus_tag="P5293_03255"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HRR48951.1"
                     /translation="MKTNLSTIGLLFVALWILSSCAPGVQLIDVEKRVAPSYPVELSD
                     KSISIFASLDEKSDSALVSNLAQGLATSLEKRLLREEGSIFVFSLKKDSSGIDSDLIR
                     DLSVESNSDLVFILSNVSIDNPSIYPNKVPGAEYHSTFLQLPFFLSLDIYDGITADAL
                     AHLYAKDTIVWEIVSRNELKQSVLNEKIKEVFASAALDIGDNISHNFFEKWESETRSL
                     YYLFGSEWEEALNAAASFDWERASEIWMKIVSSSKNKAAACAAYNLAVACEMQGDKDL
                     AYEWLSFSKKKYPLQLTGEYELQLKNNVLDK"
     gene            complement(11589..11913)
                     /gene="rnpB"
                     /locus_tag="P5293_03260"
     ncRNA           complement(11589..11913)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5293_03260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     regulatory      11970..12059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12086..>14113
                     /locus_tag="P5293_03265"
     CDS             12086..>14113
                     /locus_tag="P5293_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR48952.1"
                     /translation="MKKLTFFCGVLFMPFLTLAQTTGQNEKTTITKLDSIVVQAYRAG
                     SKTPVAHSELSVNQIRQASPVQSLPMVLSLMPSVVSLTEGGNGLGYSSIRVRGTEGSR
                     INVTLNGVALNDAESQEVFWVNIPSLTSFLQDVQLQRGVGTSTNGPAAFGASINMRTL
                     SAESLPYGLAEAGVASFNTYLSTFGAGTGLLKNGLSFDVRFSRNSGDGYIRNAKTDLK
                     SLYLSGVWLHGNSSLRLTYIMGDQCSGITWEGISREKMAVDRRYNPAGEYFDQAGNPH
                     YYDNETDNYTQQHFQLNYLHRHSQNLIFNSTIHFTLGDGYYENYKQSKKFSEYGLENQ
                     IINGVEYKKSDFIIRQAMDNSNITCLSGITYFGEKLTATGGISYSYFDGDHFGKLIWS
                     MYNSNIPDKYQWYLNTGYKNDYSLYAKGEFDFTDRIMAYVDLQYRGVLYEIKGIDKDF
                     VSMNFDKKYNFFNPKIGMTYNPNKNHQLSLSLAVGHREPSRSDIKESIKSGVADKLKS
                     ERLFDYEVGYKFESSKVAFSANFYAMEYKNQLVPTGKLSETGYVIKENVDKSYRRGVE
                     FSAAWEPFTFLKIDGNLTLSSNKILDYTLWTDMFDNSSDWNSLPQKSTFYKKTDISYS
                     PSVIGMCRGTLVNKNGYLLTLSSKYVGKQYYDNTADDTRSIPSYCTAALILG"
BASE COUNT         4155 a         2314 c         3148 g         4496 t
ORIGIN      
        1 atgggtaggg ttttatattg taagaccgga caattcgcaa tttggcagat ctctggttct
       61 gggtccattt caaggtccta ttgcctgtac aaggattgca tatggcaaag gggtttgtgg
      121 aacttcctgg aaggagaaaa ggagtctgat agttgccgat gttgataaat ttcccggaca
      181 tatagcttgt agctcatttt cgcgttcaga gattgtaatt ccgatttttg gagaatttga
      241 gagcaaggat ggagaaaatt gtaaagagcc gggcgaagtt aaagctctgc tcgatattga
      301 cagcaaattt ctggacagtt ttgacgatat tgacagagaa tatcttgaga ttttagtcaa
      361 aaacataaag ttcacataat gaatagatgg cgcttaacaa atctttgtgt gtttaccttg
      421 ttttattgtg ctttcttctt tattgcggga tgttcggtca aggtgccaaa agaggctgtt
      481 gtcggggtaa gtgaggaact tgccgaaatg aggactgtca atatctctaa tgtgcggtat
      541 aatttactgt ttgaaattcc gaaggagaga gcaaataatg ttgaggggat tctttcactt
      601 acatttgatg ttaaaaagag aacacatatc gttcttgatt tcaagcaggg agattcttcg
      661 gtaataagcg ttactgttaa tggcaagcca ggtaaatatg tttttgagaa cgaacacatt
      721 attctttctt caaggcagat tttaaaagga gaaaatcagg ttaacataaa gtttcatgct
      781 ggggatcaat cactaaacag aagagaagat cttctttata ctctattagt tcctgacagg
      841 gcaagaacgc tttttccgtg tttcgatcaa cctgatataa agagcaagta cagtctttct
      901 ctcgaaattc ctgcaggctg ggaggctgtt gctaatggac agacagtgca gagagatact
      961 attaagcaga atgatagtca atacattgtg ttttctgaaa ccgaaccaat acccacatat
     1021 cttttttctt ttgtggtcgg taaaatgaat aaaataacta aagctcagga tggcagaaaa
     1081 gtttcgatat atcaccgtga gtccgctaaa gataaaattg ctcagtgtga tgagattttc
     1141 tctcagattt tttccgcgtt gagatggatg gaggattaca caaatgtcaa atatcctttt
     1201 acaaaatatg atattattat aattcccggt tttcagtatg gagggatgga acatatggga
     1261 gcaactctct attctgacaa aaccatgttt ctgtctgaca atccgacaat agcggagaag
     1321 ctcgccaggg cagagcttgt ggctcacgaa acagcccata tgtggtttgg cgattatgtg
     1381 acaatgaagt ggtttgacga ggtgtggaca aaagaggtat ttgccaactg gtttgcgaaa
     1441 aggatggtgg ctccactttt tcctgaagtc aaccatcaat tgaatttttt gaattcatat
     1501 tttcctgctg cttatgcaga agatagaact gcgggagcga ctcctgtgag gcaggaactt
     1561 gataatctga acaatgccgg tcttgtgtat ggaaatatta tctatgataa agcaccaatt
     1621 gtaatggata aactggtgag gatggtggga gaagataatt ttaagaaagg gataagggaa
     1681 tatcttgaaa attattctta tggcaatgct acgtggaatg atcttattaa tattcttgac
     1741 aaattttttg acaaggattt aaaacaatgg agccatgttt ggataaatga agctggtatg
     1801 cctcggataa cagcaactat ttctggtgat tcaacagttt ggaagacaga tgatccaatg
     1861 ggaagaggcc tgttctggga acaacctttg agttacaaga ttgaaaattc ttatctttta
     1921 ccaaacgttg acggcaaagg ctatggttac ttcatgcttg acagggagac ttctgaatat
     1981 cttctgggca agtttgcaga ttatcgattt tcgaacacgt acaaagatac ttctgtggtt
     2041 tacaatgatc ttgcaagatg ctccttattg ataactctct atgaaaatct tttgcacaac
     2101 aatctcgatc ctgtctcttt ttgcaacgct ttgaccaaat acgtcccata tgaagacaat
     2161 ccgctgatat tgtcgagggc tttgtcatat ctttcggatg tttttgtttc ttatatttat
     2221 gagaatggct catcctttaa tgatggtggt atgcctgcaa aaattgagca aactctcttg
     2281 aacatgtttg agaacaggga gcgtatggaa gaccgtctgc ttgcattcag aactttttac
     2341 ggtatctgta gttcccggga gggggtaaat ttgctctatt caatttggaa agacccaggt
     2401 agttttaagg ctgttaagct tggagaaagg gagttgacaa atctttcata tgctgtgctg
     2461 ttgcgtctgc ctgagattta taatgaggtt aaaacaattc aagttcagcg aattaccaat
     2521 ccagacagaa aagctgaatt taattatatt ttaccgtctc tttcgggttc aaaagaggtg
     2581 agggattctg tatttcaatc tttgcttttg gagaaaaacc gacaggtgga gccatggaca
     2641 caaatgtctc tgggcttttt gaatcattat tccagacagg aaagttcttt ggaatatatt
     2701 aagcccggac tcgatatctt ggaggagata cagagaagtg gagacatttt ctttcctaca
     2761 ggatggttga gagctctttt gtcagggcat cattccgaga gagctgctgg aatcgttcag
     2821 gaatttctcg atacacacaa gaactatcct cctatgttga agagcaagat ccttcagcag
     2881 tctgatcacc tttttagaga aaaaaggtcg agtacttttg ttggcgggga agaggtggat
     2941 catcttcaaa ataagaagtg atgattagca ggcaaaggca ggctctatta atgggaggga
     3001 tggtagtcct ttgctgggct actgtagcga cagcttttaa gattgctttg gctgagatga
     3061 gctatcttca attactattg atttcatcta tcattgcctt agtaatatgt tttgttgaac
     3121 tcttgcggag cggtaagttt ggtgaagttt tgcagttatt gtgtcgcaaa aatgcccgca
     3181 aagtgggtgt tttgatgctt caggctcttc ttgccccttt cctttattat cttgtattgt
     3241 ttaaggctta ttcgttattg ccagcccaga tagcacagcc tctcaattat tcctggcagg
     3301 tggtgctgat attgatgatg tcgctttttt acaggcaaaa actttcttta aggcaactca
     3361 caggtgtttt gatttgtttt accggtgtta tatcgctttc tctcaacggg gaggctgata
     3421 tctcaggcaa actctctgtt ttgggtattg cacttgctct tggaagctcg ctgatttggt
     3481 cttcatactg gattcttaag cttaaaaacg aaatggatcc tgtggttgag ctgttcttta
     3541 attttctttt tggttctgtt tatctgataa tcatttcatt ttttactcca atggagtggc
     3601 cttctttacg ggggtttggt gctgctgttt acgttgggtg tttcgagatg ggatttactt
     3661 ttatcctttg ggccaaagct cttagaatgg ctgagaacaa gatctcgctt acacagttaa
     3721 cttatctttc tcctttgctt tctctgattt tgattcattt tattcttgga gagaatatta
     3781 cctatctcac tgttctgggg ctggctttgg tgataggggg catttatata accaataaga
     3841 gagtatctgc tccaggggaa tcttagcagg atggttctat ttaagagata catcagagat
     3901 acagcgttga aatttggcat gaaaggattt tttaataact ttgcaatccc aatatggtac
     3961 cctggccgag tggttaggca acggtctgca aaaccgtgta cagcagttcg attctgctgg
     4021 gtacctccga caggtggaag gccaaggact acagctcctc aaaattgaca tcagagaaag
     4081 gtgtaatttg ggcatcacca gaagagttct tctcattttt gccttcgact gaaggagcct
     4141 tttcttttat gaactgaatt atctcttcga gaccttgtga aaacttatca aaatcttctt
     4201 tgtacaagaa gattttgtgc ttgtcataca caaatctgcc gtctctgtcg agtctccgct
     4261 tactttcagt gattgtaagg taataatcgt tattcttagt tgccttaaca tcaaagaaat
     4321 atgtcctttt ccctgctctt accggttttg agtacacgtc atctccattg cgttcttgtg
     4381 tctctgtatt gtcaaaatct tcgaagtaca tttttgttat ttttaaattt attacacaaa
     4441 gataaaatta tttttattga gcaaaaacct atctttgtaa aataattgga ataatttata
     4501 atgattaaca gaagattgat tagggtaaag gtctttaaag tacttttcag caggatgagt
     4561 tcaggttctg aatctatgga tggtgcagaa aaggaactgc ttatgagttg tgaaaagact
     4621 ctggaacttt actattttct tcttactctg ccaaccgctc ttaagaggct ttctgaaatc
     4681 aagatcgagt cgggtctcaa gaaatttcat cctacagaag aggaagcaaa ccctaacagg
     4741 cgttttattg agaatgctgt aattgcaaag ctggagaatt gcagggagtt gaccgatttt
     4801 tgcgagaaaa agggtctttt gtggaatgat tacgactctg tcgtcaaaaa gattcattca
     4861 tcattgatgg aacgtgaata ttaccgttca tacatgaatg ctgctgaaaa cacttttgaa
     4921 gatgatctca aagttgtaat gaatattttt gaacaggaac tggaagattg tgaagatctt
     4981 tccaattctc ttgaagatgc cagtctttat tgggttgatg atcttgcata tgtcatcaat
     5041 attatattaa agaggctttc tcatctaaag actaattcta aaatatcatt tccaaaagtt
     5101 tttatcaaag aggacgacag ggagtttgcc attaggttgc ttggtaaggc aatgctcaat
     5161 tatgacgaat atgttgagct gatgtcaaag tatgttctta actgggagct ggacaggatt
     5221 gcctctacag atatgaatct gatagttctt ggaatagccg aggcaattgc ttttcccaat
     5281 attcctctta aagttaccat taatgaatat gtggaacttt caaagtttta cagcactcaa
     5341 aacagcaaga tatttgtgaa tggtatactg gacaaggttt tgctcgggtt gcaaaaagag
     5401 ggaattattc aaaaatcggg cagaggtttg gtaggctcaa tagattagct aattttgcag
     5461 aaatttaaac acatattaat atgaattttt tggtatttct tcaatccggt gcttcacaac
     5521 agggaggtgg cggaacatgg agctttctga taatgatgct cttgattttt gctgtaatgt
     5581 acttctttat gattcgtcct caacaaaaga gacagaaaga gctggcaaaa ttccgtagca
     5641 accttgccaa aggtgataaa attatcacaa tgggtgggat ttacggaatt atagtcgatg
     5701 ttaaagatca atatgtcatt gttgaggttg acaataatgt taagttaaaa gttgataagg
     5761 gctctattgt taaggatagc tctgatattc aggcagcagg taaataaatc ccttttttat
     5821 gcttaaacgg cggaagatag ggatctctta tgaaagaggc agacaagtgt tacttttctt
     5881 gttctgtctc tttctggcat ttgttttgtg ggcaattcgc aatctttcag attattacag
     5941 cgttaatctt aattactccg tccatgttgt gactcagaaa agaggtcatt ctgcagaagc
     6001 tttttctgac aataaacttt cggttacagg cagagcttcc gggttctata ttctacagaa
     6061 cagacttggc attcaaggta acctgattac tttatctgtt ccaggctata tgcttaagcc
     6121 tctttctgga acagaggata aattcttttt cgttgttagt gaagtaaggg atataattac
     6181 tgatgcactt agtggacatg tcgagattga atccttctct tccgatactc tctatctgac
     6241 tttacctcgt cagtcacaca gaaatattcc tgttgctgta gattataagg cgagctttaa
     6301 ggatcagcac atgcctgtgg gaaaaataaa gatatctcct tcaactgtta ctgtttacgg
     6361 agatatctct ctgctccaga acattgattc tgtccgtaca aaacatctta cctttgaacg
     6421 actctcttct actagaagtg gaatagtgga acttttacct gttagtggat tgcgttttga
     6481 gactaaagag atttattaca ctcttgaagt tgtaagatat gttgaaaatt cgactactat
     6541 tcctgttgaa tcggtaaatg tgccagaagg taagtatgtg aaattctttc ctaatactgt
     6601 cagggttact tacagaatcc cttttagtaa taaacaaaac cgggaaaata ctatctttgt
     6661 tgctacgatt gactacaagg aaataactgc ttcaataagc aacactgttg aaccaatggt
     6721 ttcaggactg ccggaaaatg ttctttctgt ggaactggac cctaaattca ttaagtgtaa
     6781 gattattgag caataaatca aaatagtatg ttttgctttg cctgtacagg gggtattggt
     6841 gcaggaaaat cttatgtaat aaggattttt tctgctttag gagttcctgc ctatgtggct
     6901 gacgacaggg tgaaatatct gtatgatgtt gacaggaacc ttattgataa gcttgttgat
     6961 ttacttggca aggagatagt gactgatggg gtgctgcaaa gaaaggttat ggcaaaaata
     7021 attttttctg acaggaatct tctggagaaa gtcaataata ttgttcatcc caggttgttg
     7081 gaagattttt atgagtggag ggagcagaaa agatcagaag gcattgaaat tattatttac
     7141 gaatcagcaa ttttttttaa aactcctatt ttttggcatt tggcagacaa agttattgtg
     7201 gtttttgcgc cggaaaatgt tagaatcatg agggttatga aaagggatgg tttaagtgag
     7261 gaacaagtac gggagagaat gagacatcaa atggattatg acagtatgat agcaagggcg
     7321 gattttgtta tttatactga tggcaagaga gctgtgttgc cacaagtgtt gagtattttg
     7381 gaaagtgtca agtataagtg ttgaaatggg atacggtaaa tggattacag gagccctggg
     7441 atgggcattg ggaggaccga ttggtggaat aataggtttc gctataggtt caatttttga
     7501 aaaaggcaaa cagattgagc aatatcaaca gcaagaaccg tctgccggtc gtcgttactc
     7561 aaatatagag caaagaaaca gttttcttgt ttcacttctt gttctttctt ctgcagtcat
     7621 gaaggccgac ggaaggataa tgcagtcaga actggagtat gtcaaaagat ttatcaggca
     7681 gaattttggt gaagatgcag aaagagaggc atctcttttt ttaagagatt tgcttaaaaa
     7741 ggatgtagat attaatgccg tgggcttgca gattcgcaat tacatgaatt atgaggccag
     7801 aatccaattg cttcactatt tgacaggcat tgctcaggca gacggctctg taagttcgtc
     7861 tgaactggat gttctaagaa ctatttctgc cggtttgggg atctcaagag cagagagtga
     7921 gtccatattt gcaatgtttg acaacggact tgaagctgcc tacaaagtgc ttgaaattga
     7981 caagaacgca acagacgatg aggtgaaaaa agcctacaga agattggcta caaaacacca
     8041 tccggacaag gtatcaaatc tcggtcctga tgtccagaaa gctgctgaag agcgtttcaa
     8101 gagtatttcc gaagcatatg aaaagataaa aaaagataga ggaattgttt aaagattagg
     8161 atgatgaaaa caaatttaaa gaaaattctt tcgatttcag gccaaccagg gctttataac
     8221 tatttgtcga acgcaaatgc cggagttatt gtggaatcac tgttaactaa aaagaaaact
     8281 atttgtggtt cttctttcag acttacagcg ctttctgata tctctattta tacgtcagat
     8341 gatgagctgc ctttgcacgt tgtttttacg aaaatgcatg aaaagcttgg agatcagagt
     8401 gctccaagtg gaaaatcttc acctgacgaa ttaaggactt tttttgaaga ggtaattcct
     8461 aactatgaca aagttcgttt ttatccgtct catatgaaaa aggttgtcga ctggtacaat
     8521 gtccttaaag acaatgcctc actcgacttt gaattgcctg atgaagaaaa gcaaaattaa
     8581 gctaataact ctcggctgct ctaaaaacag ggttgattcg gagcatcttc ttaaacaagt
     8641 agagaagtca ggcattgaga tatctcctga aagtgagtct gtagaagacg cacgagcgga
     8701 tgtcttaatc ataaatacct gtggatttat taaagatgcc aaggttgagt caattcaggc
     8761 tattttggaa gcagttgaag ccaagaagca tggatttact tctaaagtcc tggtctttgg
     8821 ttgtttgtct caaagataca gggaggagtt ggttaggtca attcccgaag ttgatggatt
     8881 ttatggtgtg tttgacattg aacaggttgt tgctgcttta ggttcttgct ggaatagcaa
     8941 ttatgaaact caaagagtct tgactactcc atcacattat gcatatctta aaatatcaga
     9001 aggttgtgac agaacgtgtt cctattgttc tattcctatt ataaagggaa agcatgtttc
     9061 agtgcctgtt gaaaagcttg taagtgaagc acaatatctt gctttacttg gggttaagga
     9121 gcttattctt gttgctcagg atactactta ttatggagta gatctgtatg gaaaaagatg
     9181 tatagctgaa cttgtaaaga gactttcaga tatagaaggg attgaatggc tgaggttgct
     9241 ttatgcctat cctgctgctt ttccggagga tttgctcgag gttatggcaa ataattccaa
     9301 ggtgtgtaaa tatttggata ttcccctgca gcatgtttct gacaaagttc ttagaaatat
     9361 gaaacgttct gtgaatggag agcagactcg cagactcgtg gagatgtttc ggagaaatgt
     9421 tcctggtatt gttttgagga ctacgatgat ggttggtcat ccaggagaag acaataaagc
     9481 ttttgaagaa ttattgtctt ttgtttcaca ggctagattt gagagattag gtgcatttac
     9541 ctattcggag gaggaaggta cttggagcgc tcataacctt agagatactg ttcgtgctga
     9601 ggtcaaagag gaaagatata aacgcctcat ggaattgcaa tcgggagttt ctatggaata
     9661 taacgagtcg agaataggaa caaaagagaa agttttggtt gattctgtgt ctgacaggta
     9721 tttaatagca aggagcagaa atgaaagtcc ggaggtggat ggagagattt tgatagatag
     9781 aataattaat tcctcgaatt taaattctaa agatttaatt ggtacatttg ttgatattga
     9841 gattacagat gcagatgagt atgatctgta cgcaaagttg atattataaa ttaaatagct
     9901 acattatgaa attacttgat ggaaaggttg ctctcattac aggagctgca agggggatag
     9961 gaaaggcaat cgccttaaag tttgcttcag aaggagcaga tattattttt accgaccttg
    10021 tgattgatga aaatgcagag acaacaaaac gtgaaattga atcttttggt gttaaagtac
    10081 ttgccctggc ttcaaatgct tcttcttttg aggaagctca caaagttgtg gatgagggcg
    10141 taaaggtatt cggcaagata gatattctgg taaacaatgc cggtattacc aaagatggac
    10201 ttataatgag gatgactgaa cagcagtggg atgctgtaat taatgtcaac ctcaagtctg
    10261 catttaatta tattcatgcg ataacacctg ttatgatgag acagaagagt ggcagtatta
    10321 tcaacatgag ctctgtggtg ggtgtttcag gtaatgcagg gcaggcaaat tatgcagcct
    10381 ccaaggccgg gatgataggg cttgccaaat ctgttgcaca agagcttggt tcaagaggaa
    10441 tcagggcgaa tgctgttgct cccggtttta ttttaacaga tatgacagaa aaattacctg
    10501 atgcagttaa aaaagaatgg gcagaaaaga tacctcttaa aagaggtgga cttcctgaag
    10561 atattgcaaa tgtttgtact ttccttgcct ctgatttatc ttcatatgtc accgggcaag
    10621 tgatatctgt ttgtggtggc atgaaaatgt aaaaaaagaa tatcatgaaa accaaccttt
    10681 caaccatcgg attgttattc gttgctcttt ggattctttc ttcctgtgca ccgggtgttc
    10741 aattgattga tgtggagaaa agagtggcgc caagctaccc ggttgagtta tcagacaaaa
    10801 gtatttcaat ctttgcatca cttgacgaga aaagtgattc agctttggtt tccaaccttg
    10861 ctcaagggtt agctacttct ttggagaaga ggcttttgcg agaggaaggt tctatttttg
    10921 tttttagttt gaaaaaagat tcttccggga ttgattctga ccttatcagg gatctttctg
    10981 ttgagtcaaa ttcagatctt gtctttattt tgagtaatgt ctcaattgat aatccaagta
    11041 tatatcccaa taaagttccg ggagctgaat atcattctac ttttcttcag ttgccatttt
    11101 ttctctctct ggatatctat gacggtataa cagctgatgc acttgctcat ttgtatgcaa
    11161 aggatacaat tgtatgggaa attgtttcaa gaaacgaatt gaaacaaagc gtgcttaatg
    11221 aaaaaatcaa agaagttttt gcgagtgctg ctctagatat aggagacaat atctctcata
    11281 acttttttga aaaatgggag agtgaaacaa ggagtttata ctatctattt ggttcagaat
    11341 gggaagaagc acttaatgca gcagcttctt ttgattggga aagagcatct gaaatttgga
    11401 tgaagatagt ttcatcttcc aagaataaag cggcagcttg tgctgcttat aatttggcag
    11461 tagcttgcga gatgcaggga gacaaagatc tggcttatga atggctttct ttttcaaaga
    11521 agaagtatcc tcttcagctt actggagaat atgagctgca actcaaaaat aatgtcctag
    11581 ataaataaaa aaaaggtggg tctgtaagcc ggattctgtg cctcattgag gcctctatca
    11641 tttatctgat gcagcctacc ccccggcatt atcggacgag cagtcctgag ccggtataca
    11701 tggccttgca ggagactggt cgtacccaaa atatgtcacc atattttggc gtgggctctt
    11761 accccgcgtt ttcaccctta cctcacaaga ggcggtaatt ttctgttacg atagccctaa
    11821 ggttacccct agctgtgatt tccacagagt cttgctctac cctgtccgga ctttcctccc
    11881 cctttgtgag agagcgatag agcgacccac ctattacaaa gttaaataaa aagtgtcttc
    11941 tgtgacttat tttatatctt tgcatcacta ataatacttg ggggtgccgt ttcggctgag
    12001 attataccct ttaacctgat ctggataatg ccagcgaagg gaaagcgtct ttttcttata
    12061 tccccctgct gataaatatt taattatgaa aaaattaact tttttctgcg gggtactttt
    12121 tatgcctttt cttacccttg cccaaactac agggcaaaat gagaaaacaa caattacaaa
    12181 actggacagt atagtggtgc aagcttacag agctggcagt aaaactcctg ttgcccattc
    12241 agagctgtct gttaatcaga ttagacaggc gtcacctgtc caatcattac caatggtcct
    12301 ttctttaatg ccgtctgtcg ttagtttgac tgaggggggc aatggattgg ggtattcatc
    12361 gattcgtgtt agaggcacag agggttcaag aatcaatgtg actctcaatg gtgtagcact
    12421 caatgatgca gaatcccagg aagttttttg ggtgaatatt ccttcgttaa cttcattttt
    12481 gcaggatgta cagcttcaaa gaggtgtagg aacatccact aacggtccgg cagcttttgg
    12541 tgcaagtatc aatatgcgta ctctgagtgc tgaaagcttg ccatatggtt tggctgaagc
    12601 aggagttgca agctttaata catatctttc aacattcgga gctgggacag ggttactcaa
    12661 gaatggtctt tcttttgatg tcaggttctc ccgtaattcc ggggatggtt atattagaaa
    12721 cgctaaaact gatcttaaat ctctttattt gtcaggagta tggttacatg gaaattcatc
    12781 cttaaggctg acttatatta tgggagatca atgttccggc attacctggg agggtatcag
    12841 tagagaaaaa atggcagttg acaggagata caatcctgct ggagagtatt ttgatcaggc
    12901 aggaaatcct cactattatg ataatgagac tgataattat actcaacaac attttcagct
    12961 taattatcta catcgtcatt cgcaaaatct gatatttaac tccactattc acttcacact
    13021 tggtgatggt tattatgaga attataaaca gtctaaaaaa ttttcagagt atggacttga
    13081 aaatcaaatt ataaacgggg ttgaatacaa aaagtctgat tttattatca ggcaggcaat
    13141 ggataattct aacattactt gtttgtcggg tataacctat tttggagaga aacttacggc
    13201 aacaggcggg atatcttatt cttatttcga cggagatcat tttggaaaac ttatctggtc
    13261 tatgtataac agtaatattc ctgataaata tcaatggtat ctcaacacag gttacaaaaa
    13321 tgactattct ttatatgcaa agggagaatt tgattttaca gataggatta tggcttatgt
    13381 tgatttgcag tacagaggag ttttatatga gatcaaaggt atagacaaag attttgtatc
    13441 aatgaacttt gacaagaagt acaatttttt taatcctaaa atagggatga cttacaaccc
    13501 aaataaaaat catcaattat ctttatcact tgctgtaggt cacagagagc caagtcgttc
    13561 ggatattaaa gaatccatta agtcaggagt agcagacaag ctgaagtctg aaagattgtt
    13621 tgactatgag gttggttata agtttgagtc atcaaaagtt gcattctctg caaactttta
    13681 tgctatggag tataaaaatc agcttgtccc tacaggtaag ttgagtgaga cgggatacgt
    13741 tatcaaggaa aatgtcgaca aatcttatag gaggggagtg gaattttcgg ctgcatggga
    13801 gccttttaca tttttgaaaa ttgatggtaa tcttactctg agcagtaaca agatccttga
    13861 ttatacttta tggactgaca tgtttgataa ttcatctgat tggaattctc ttccacaaaa
    13921 gagtacattt tataaaaaga cagacatctc ttattcaccg tctgttatag gaatgtgtag
    13981 gggaacgtta gtaaataaaa acggatatct tcttactctt tcttcaaagt atgtagggaa
    14041 gcaatattat gataacactg cagatgatac gagatctatc ccttcatatt gtactgcagc
    14101 tttgatttta ggc
//
LOCUS       DAOTQL010000032        13929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4171_length_13929_cov_5.612945, whole genome shotgun sequence.
ACCESSION   DAOTQL010000032 DAOTQL010000000
VERSION     DAOTQL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4171_length_13929_cov_5.612945"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..1853)
                     /locus_tag="P5293_03270"
     CDS             complement(54..1853)
                     /locus_tag="P5293_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48953.1"
                     /translation="MIVKMDKYMLLIFHQDLQMFLEHLQELGMVDIRRQGHAVDGKSK
                     ELSDIIQRYRRTIKKLKETKNTLKDQEIDNSLIDLKSPVEELFQECEKAYSDNSLLEM
                     ELQLLLRDKEEAALWGDYNKGDISKLKDLGFDLHFYSISESKFRKEWEKDYILYELNR
                     ANGKVFFVILSPKGSEIKFKIPESKFPQTPLHALEERENELRKQISLNHKKMIAYTFC
                     VEMFQSKLNELNMELERYLATVSSQKEVEGLVDTLTGFAPAESRDDVCKFLDDEGIYY
                     EVVEAVEEDKPPIKLKNNFFTRLFEPIGALYMYPKYGELDLTPFFAPFYMLFFGFCLG
                     DMGYGIVLMIIGGLAKLKFPNFKSILTLVQLLGLGAFIMASFTGTFFGGKLYDWLPLP
                     DSIQSFFFSDIKMFWFAIIFGLVHIVFARLINAIDSIIRKGWKYGMSNIGWAIVITWA
                     ATAYASTMIPEMVLPKWFNYLAICGAALILFFSETSGNIFIRMLKGTFSFYDITGVFG
                     DTLSYIRLFGLGTSGGILGMVINSVAFSLTAIPYVGWFFTILILIVGHIAVLALSSLG
                     AFVHPMRLTFVEFYKNAGFTGGGKEFKPLKKIN"
     gene            complement(1850..2452)
                     /locus_tag="P5293_03275"
     CDS             complement(1850..2452)
                     /locus_tag="P5293_03275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRR48954.1"
                     /translation="MAIKFQFNKTSLNDLNKQLKIRTNALPTLKNKESALRFEVKRAK
                     YRSDELANRLESVLRSYQHICGLWNEFRPDLIKVKDVNLETVKVAGVKTPSLKEIIFE
                     ETPFNFFTQPFWYNEGINILKELSSLGIESEIYREKMRLLDFSRKKTTQKVNLYEKVQ
                     IPGYQEAILKIKRFIEDEENLSKASQKIVKNRHIAEEEGI"
     gene            complement(2466..3785)
                     /locus_tag="P5293_03280"
     CDS             complement(2466..3785)
                     /locus_tag="P5293_03280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRR48955.1"
                     /translation="MEGRAFTKIYTQLESITKATVSLRAKGIANDELASVGGRLAQVV
                     KIKGDLVTLQVFAGTEGIPTNAEVSFFGDPPYLKVSEELAGRFFNAYGEPLDNGPAIE
                     GEKRYLGGPSVNPFRRKQPSELIPTGIAGIDLNNTLVSGQKIPFFADPDQPYNQVMAM
                     VALRADVDKIILGGMGITNDDYLFFKQSFENAGALNKIISFVNTTEQAPVERLLIPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQLPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRTDSDTGKVIVDPFR
                     SLSRLKQLVIGKKTREDHNYVMNAAVRLYADAANAKTKLENGFDLSDYDNRCLEFAKE
                     YSVRLLAIDVNINTTEMLDTAWELFRKYYTPTELGIKESIMNKYWNN"
     gene            complement(3775..5526)
                     /locus_tag="P5293_03285"
     CDS             complement(3775..5526)
                     /locus_tag="P5293_03285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRR48956.1"
                     /translation="MKTTGKVTGIIANLVTIEVDGPVAQNEICYIDLSGTKLMAEVIK
                     VNGKEAFVQVYESTRGLKTGDSAEFEGHMLEATLGPGLLSSNYDGLQNNLETMEGVFL
                     RRGEYTSPLDMEKLWHFTPIANPGDEVTAADWLGEVKEGWLPHKIMVPFSYKEQYKVK
                     YIAAEGDYKVSDKIAVVIDENGLEHEITMMQKWPVKVAITAYKDKPRPFRIMETGIRC
                     IDTFNPIAEGGTGFIPGPFGCGKTVLQHAISKQAEADVIIMAACGERANEVVEIFTEF
                     PELIDVHTGRHLMERTTIICNTSNMPVAAREASVYTAMTIGEYYRSMGLRVLLLADST
                     SRWAQALREMSNRMEELPGADAFPMDLPAIISNFYSRAGIVNLNNGQTGSVTFIGTVS
                     PAGGNLKEPVTESTKKAARCFYALSQSRADKKRYPAVDPIDSYSKYLEYPEIIGDLCS
                     RVNKDWVDKVMKGKNIVLRGKEAFEQINILGDDGVPIEYHRKFWKSELIDFIILQQDA
                     FDKIDSVTSLERQEFMYSLIIDICDKDFEFEDFEECANFYKKLINILRQMNYSEFKGD
                     NFNKYLEQLKEEINNGR"
     gene            complement(5535..6170)
                     /locus_tag="P5293_03290"
     CDS             complement(5535..6170)
                     /locus_tag="P5293_03290"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HRR48957.1"
                     /translation="MKNYEFIIAGLPYIEPDFDNISIDYDSQVSQIISSLDDKDRRYV
                     DWLMFGLEGDNLSPHFYRVISKNKIKFLRLYFGFDLMLRNIQAAYISRKRNVDPSPYL
                     VGENDIVEALKKNKAPDFGITSEIEFAGKLLQIFEMSDILEREKALDLLRWEEAEKIC
                     VFNYFDMNVILSYILRAFILKRWRSMDKSQGEMLFRKYVEEMKNSYKNNIE"
     gene            complement(6176..6790)
                     /locus_tag="P5293_03295"
     CDS             complement(6176..6790)
                     /locus_tag="P5293_03295"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRR48958.1"
                     /translation="MQNKLQELTEKLYSEGLSKGREEAEKIKADAVKEAELIISKAHE
                     ESNKIIATAQKEAEDIKERVLNEVKMSSRQSYTVLKHEIENMIIANAIGKPVSEAMSD
                     SSFIKSLIQTIVNAFSQTSDKPADLSIILPEKMKGDLDNYIVNSIAQQFKGGIEIRFD
                     HKLSSGFKIGPKAEGYYISFTDKDFEELIGTYLRPRIREIIFAK"
     gene            complement(6959..8116)
                     /locus_tag="P5293_03300"
     CDS             complement(6959..8116)
                     /locus_tag="P5293_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRR48959.1"
                     /translation="MENGSLSLIGENLRQFIQYTGFANVTFNHLIMIVIGLAFIYLAI
                     KKQWEPMLLVPIGFGIIVGNIPFFPGLSIGVYEQGSVLNILYQGVIQGWYPPLIFLGI
                     GAMTDFSSLISNPKLLLIGAAAQLGIFGAYAIALLMGFSASDAGAIGIIGGADGPTAI
                     FLSSRLAPNLLGAIAVSAYSYMALVPVIQPPVMRLLTNKKERLIRMKPPRAVSQTEKI
                     LFPIIGFLLTCFIVPSGLPLLGMLFFGNLLKESGVTRRLAETARGPLIDVITILIGLT
                     VGASTQADKFLQLTSIYIFILGALSFIIATTGGVLFVKFFNLFLKEGNKINPLIGNAG
                     VSAVPDAARVSQVIGQEYDKTNHLLMHAMGPNVAGVIGSAVAAGILLGFLG"
     gene            complement(8118..8561)
                     /locus_tag="P5293_03305"
     CDS             complement(8118..8561)
                     /locus_tag="P5293_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRR48960.1"
                     /translation="MKEYKLKINGNAYSVTVSDIEENLAEVEVNGIPFKVEFENPIKK
                     NIVTPPKTLSSVVSPSSTVQKPSVQVTPPSSSTVINVTSPLPGIILEVAVKEGDIVKK
                     GQKLIVLEAMKMENVIEASADGKIISVKVSKGDSVLEGATLVTIG"
     gene            complement(8578..9486)
                     /locus_tag="P5293_03310"
     CDS             complement(8578..9486)
                     /locus_tag="P5293_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRR48961.1"
                     /translation="MRIISKVFLLLGFMSITLTLGAQSQNDIRINEFLVHNTNDFEDD
                     FGHKNGWIELFNSSYGTVNIGGCYLTNDPSNLTKYIIPKGDVLTKIQPRQHVLLWADN
                     EPFRGTFHINFKLEDSQEILFVASDGKTIIDRVKIPHDLLDTNVSYGRTYDGVGSTDG
                     SEGWQVMKRTSPSTNNSGVDDVPKSVLVKKADPYGIIMSITAMSVVFIALILLYIIFK
                     QVGNYHIKKSRDRSLVSLSDSDKPKATNVSAEIYAAIAAALHSYLRGNETHDVENTVL
                     TISRVTKNYSPWSSKIYTLRKTPQKK"
     gene            complement(9505..11058)
                     /locus_tag="P5293_03315"
     CDS             complement(9505..11058)
                     /locus_tag="P5293_03315"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRR48962.1"
                     /translation="MSQQLEQVKALTQLREKARLGGGQKRIDSQHSKGKFTARERIAM
                     LLDEGSFEEFDMFVTHRCTNFGMEKERFLGDGVVTGFGTIDGRLVYVFAQDFTVFGGS
                     LSESFAMKICKIMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVI
                     PQISAIFGPCAGGAVYSPALTDFNIMTKGTSYMFLTGPKVVKTVTGEDVTQEELGGAS
                     VHSSKSGVAHFAVNSEEEGIQVIRDLLGYLPQNNLEDPPMTENTDPIDRLEDSLNEII
                     PDSPNKPYDMYEVIGSIVDNGKFLEVHKDYATNIIVGFARFNGASVGIVANQPKVLAG
                     VLDINASRKAARFVRFCDAFNIPLVTLVDVPGFLPGTQQEYGGIILHGAKLLYAYGEA
                     TVPKITVTLRKSYGGAYCVMSSKHLRGDINYAWPSAEIAVMGPSGAIEVLYGKEMATA
                     ENPEKFAAEKEAEYREAFANPYNAARYGYIDDVIEPRNTRFRIIRALQQLTTKKLTNP
                     PKKHDNLPL"
     gene            complement(11086..11505)
                     /gene="mce"
                     /locus_tag="P5293_03320"
     CDS             complement(11086..11505)
                     /gene="mce"
                     /locus_tag="P5293_03320"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRR48963.1"
                     /translation="MKLSHIEHIGIAVKSLDTAIPYFEKVLGLKCYAVEEVKDQKVKT
                     AFFKIGETKIELLESTDPEGAVGKFIESRGEGVHHIAFAVEDGVQNSLNELETKGIRL
                     IDKSPRNGAENLKIAFLHPKSTFGVLTELCEDPSKSK"
     gene            complement(11528..12244)
                     /locus_tag="P5293_03325"
     CDS             complement(11528..12244)
                     /locus_tag="P5293_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRR48964.1"
                     /translation="MGRAFEFRKERKFKRWGNMSRTFTRLGKEITIAAKSGGPDPDNN
                     PKLRAIISTARSENMPKENIERAIKRAVEKEHSEYKEVVYEGYGPHGIAFLIETATDN
                     PTRTVANIRSYFNKFNGSLGTQGSVDFMFDKKCVFRIRSSKPVNLEELELELIDYGGE
                     EMFMEDDNIVIYGAFEAFGAIQKYIEEGGYELVSGGFERIPNTELKELNEDQKAEIEK
                     LIERFENDDDVQNVYHTMAV"
     gene            complement(12268..12930)
                     /locus_tag="P5293_03330"
     CDS             complement(12268..12930)
                     /locus_tag="P5293_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR48965.1"
                     /translation="MSKKKKNEQEAVSEVVNQTEHFIERFKKPLLYSVITILAITAII
                     LGYNQFYRKPKTNEAMAQSFVAERYFRADSFALAFNGDGNALGFKQIIEEYGDKSGAA
                     VYLYAGICELQLGQYKEAIEYLKKYKGKDPILQARALCNIGDAYAFLENNKEALNYYN
                     KAAKLADNDYAAGYLLKAGIMYEELGDIDNALKTYEEIKAKYPNTIEGYDIDKYIYRI
                     KK"
     gene            13038..>13929
                     /gene="recF"
                     /locus_tag="P5293_03335"
     CDS             13038..>13929
                     /gene="recF"
                     /locus_tag="P5293_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRR48966.1"
                     /translation="MYLKKITINNFKNISEAQIILSPKINCITGINGSGKTNLLDAVY
                     YLSMTKSFFSSIDHFSIRYNTDSATINGEYVRENNNGVDIISLSFNINGEKHLKRNSR
                     SYSRFSDHIGLLPIVMVSPADTSLVNESGDERRKFMNAILSQIDKEYLRRLQNYNQLL
                     AQRNRLLKGDVVSDLLLSTLNEQMSVNAEYVYLKRKDFINKMVPVIKDYYDMLSGDRE
                     EVNIEYKSDLHTCSLDELFSRNLEKDKFLGYTSSGIHRDDLNFTMDGYPIRRGGSQGQ
                     QKSFLIALKLAQFTVMKNIHC"
BASE COUNT         4128 a         2781 c         2434 g         4586 t
ORIGIN      
        1 attaaaggca atgtttgttc cataattata ttatttcagt ttaatttatt atattaatta
       61 atttttttaa gtggcttaaa ctctttacca cctccggtaa atccggcatt tttgtaaaat
      121 tcaacaaatg taagacgcat aggatgtaca aacgctccaa gacttgagag tgcaagtact
      181 gcaatatgac ctacaatcag aataagaatt gtaaagaacc atccgacata aggtattgct
      241 gttaagctaa aagctaccga gtttattacc attccaagaa ttcccccgga tgttccaagc
      301 ccaaagagtc gaatgtagga aagagtatct ccaaacacac ctgtgatgtc ataaaaagaa
      361 aatgtccctt tcagcatgcg tataaaaata tttccgcttg tttccgaaaa gaagagaata
      421 agtgcagctc cacatattgc aaggtagtta aaccattttg gaagaaccat ttcaggaatc
      481 attgtgcttg catatgctgt agctgcccaa gtaattacga ttgcccagcc tatgttgctc
      541 atcccatatt tccatccttt tctgattatt gagtctatag cattaatcaa cctcgcaaaa
      601 acaatatgta cgagaccgaa tataatggca aaccaaaaca ttttgatgtc agagaaaaag
      661 aaagactgta tagaatcggg caaaggtagc cagtcatata attttcctcc aaaaaaggtt
      721 ccggtaaaac ttgccataat aaatgctcca agaccgagta actgtactaa agttaaaata
      781 cttttaaagt ttggaaattt taatttagcc aatcctccaa taatcatcag tacaattcca
      841 tatcccatgt cacctaagca aaaaccaaaa aacaacatgt aaaatggtgc aaagaaagga
      901 gtaagatcaa gctctccata ttttggatac atatataaag cgcctattgg ttcaaacagt
      961 cttgtaaaga aattgttttt aagctttata ggtggtttgt cttcttcgac ggcttctaca
     1021 acctcataat atattccttc atcatcaaga aacttgcata catcatctcg agattctgca
     1081 ggtgcaaaac cggtaagagt gtctaccaaa ccttctacct ctttttgaga cgaaacagtc
     1141 gccagatatc tttctaattc catatttaac tcattgagtt tcgactgaaa catttcaaca
     1201 caaaaagtat aagctatcat ctttttgtga ttgagagata tttgttttct aagttcattt
     1261 tccctctctt caagagcatg taatggtgtt tgaggaaatt tactctcagg tattttgaat
     1321 ttaatttctg aacctttagg tgaaagaata acgaagaaaa ctttaccatt agccctgtta
     1381 agttcataca gaatataatc tttttcccat tcttttctga atttactttc agaaattgag
     1441 taaaagtgga gatcaaaacc caaatctttc agttttgaaa tatctccctt attatagtct
     1501 ccccaaagtg cagcttcttc cttgtcgcgt agtagcagtt gaagttccat ttcgagcagg
     1561 gaattgtctg aatatgcctt ttcgcattcc tgaaacagct cttcgacagg agatttaagg
     1621 tctattagtg agttgtcaat ttcttgatct ttaagagtgt ttttggtttc cttaagtttt
     1681 ttgatagtgc gtctgtatct ctgaataata tcactcaact cttttgattt gccatccaca
     1741 gcatgacctt gacgtctgat atctaccatt cctaattcct gaaggtgctc cagaaacatt
     1801 tgaagatctt gatggaatat cagcaacata tatttatcca tctttactat catatccctt
     1861 cctcctcagc gatgtgtctg ttctttacta ttttttgaga ggcctttgaa aggttttcct
     1921 catcttctat aaatcttttg attttaagaa tagcttcctg ataacccggg atctgtactt
     1981 tttcatacag atttactttt tgtgtggttt tttttcttga gaaatccaga agtctcatct
     2041 tttctctata aatttcggat tcaataccaa gagatgacaa ttcttttaag atattgattc
     2101 cttcgttgta ccaaaatggc tgagtgaaga aattaaatgg cgtctcttca aaaataatct
     2161 ctttgaggct tggtgtcttg actccggcaa ctttgacagt ctccagattt acatctttta
     2221 cctttataag gtcaggccga aattcattcc acaacccgca gatatgttga tatgatctga
     2281 gcactgattc aaggcggttt gccaactcgt cagaacgata cttcgctctt ttaacctcaa
     2341 acctcagagc agactccttg ttcttgagtg taggcaaagc attggttctg atcttgagct
     2401 gcttgttcag atcattcagt gatgttttgt tgaattggaa tttaattgcc atgattaata
     2461 aactgtcagt tattccagta tttattcata atagattcct taattcccag ttcagtagga
     2521 gtgtagtact ttctaaacag ctcccaggct gtgtcaagca tttctgttgt attgatgtta
     2581 acatcgatag cgagtaatct tacagaatac tcttttgcaa attccaggca tctgttatcg
     2641 tagtcgctaa gatcaaatcc attttcaagt ttcgttttgg catttgcagc atcagcatat
     2701 agacgaacag ccgcattcat aacataattg tgatcttctc ttgtcttttt gccgataact
     2761 aactgtttca gacgagacaa agaacgaaac ggatcaacaa taactttccc tgtatcagaa
     2821 tcagtcctca ggaatagctg accttcagtt atataaccag tattatcagg gattgcatga
     2881 gtgatgtctc caccactcaa tgtggtgacg gcaataattg ttatggaacc tccatcagga
     2941 agttgtacgg ccttttcata tatttttgca agatcagaat aaagagatcc cggcattgaa
     3001 tccttggacg gaatttgatc cattctgttg cttacaatac ttagtgcatc agcataaaga
     3061 gtcatgtctg taagcaaaac caagactttt tcatttttat cgactgcaaa atattctgct
     3121 gcagtaagtg ccatatccgg gatcaaaagt ctttccacag gggcttgttc ggtcgtattt
     3181 acaaaactga tgattttatt aagagctcct gcattttcaa aactctgttt aaaaaagaga
     3241 tagtcatcat tagtgattcc catgcctccg agaatgatct tgtctacatc ggccctaagg
     3301 gcaaccatgg ccattacctg attataaggt tgatccgggt ctgcaaaaaa aggtattttc
     3361 tgaccggaaa ccaaagtgtt attcaaatct atgccggcaa tacctgtagg aataagctca
     3421 gaaggttgtt ttctcctgaa agggttaacg gagggaccac caagatatct tttctcacct
     3481 tcaattgccg gtccattgtc gagaggttcg ccatatgcat tgaaaaatct gcctgctaac
     3541 tcttcgctga ctttaagata aggtggatct ccaaaaaatg acacctctgc atttgtcggg
     3601 ataccttctg ttccagcaaa tacttgtaaa gtaacaagat ctcccttgat tttaactact
     3661 tgagccaatc tacccccaac agaggcaagt tcatcattag ctataccttt tgctctaaga
     3721 gatacagttg cctttgtaat agactccagt tgagtatata tctttgtgaa tgctctacct
     3781 tccattgtta atctcctctt taagttgttc cagatatttg ttgaaattat cgcccttaaa
     3841 ctctgaatag ttcatttgac gtaatatatt tatcagtttt ttatagaagt ttgcacactc
     3901 ttcaaaatct tcaaattcga agtctttgtc acaaatatca attatcagag agtacatgaa
     3961 ttcctgtctt tcaagagatg tgacagagtc aattttgtca aaggcgtctt gttgtaaaat
     4021 tataaaatca ataagttctg acttccagaa ttttcggtga tactctatag gtactccgtc
     4081 atcacccaga atattgattt gctcaaaagc ctctttgcct ctaaggacta tattcttgcc
     4141 cttcataacc ttatcaaccc agtctttatt taccctgctg cataaatcgc caattatttc
     4201 agggtactca agatattttg aataagaatc tatagggtca actgcaggat atcttttctt
     4261 gtcggcacgg ctttgagaaa gggcgtaaaa acatcttgcg gctttttttg tggattcggt
     4321 aacgggttct ttcaagttac caccagcagg agaaacagtg ccgataaatg ttaccgaacc
     4381 tgtttgccca ttgttaagat taacaattcc ggcccttgaa tagaagttgg aaataatagc
     4441 cggaagatcc attggaaaag catctgcacc aggtagttct tccattctgt tactcatctc
     4501 tcggagagct tgagcccagc gagatgtaga atctgccaaa agaagtactc ttaaccccat
     4561 tgatctgtaa tattcgccaa tggtcattgc agtgtatacg gaagcttctc gggcagcaac
     4621 aggcatgttg gaggtattac atataattgt tgttctttcc atcaggtgac ggcctgtatg
     4681 aacatcaatt agttccggaa attcagtaaa gatctcaact acctcattgg cgcgttcacc
     4741 acaagctgcc ataattataa catctgcttc tgcctgtttg gaaattgcat gttgaagcac
     4801 agttttacca catccgaatg gtccagggat gaatccagtt ccgccttcag caatgggatt
     4861 aaatgtatct atgcagcgta tacctgtttc catgattctg aatggtcttg gcttatcctt
     4921 gtatgcagtg attgcaactt ttacaggcca tttctgcatc atggtaatct catgttccaa
     4981 accattttca tcaataacaa ccgctatttt gtcagagacc ttataatcgc cttcagctgc
     5041 tatgtattta accttatact gttctttata tgaaaaagga accattattt tgtgaggtag
     5101 ccaaccctct ttgacctctc cgagccagtc cgctgctgta acttcatctc ccgggtttgc
     5161 aatcggagta aaatgccata atttttccat atcgagtgga gatgtatatt cgcctctcct
     5221 aagaaagaca ccctccatgg tttcaaggtt attttgcagg ccatcgtaat tgcttgaaag
     5281 gagtccgggt ccaagtgtag cttccagcat gtgaccctca aattcggcag agtctccagt
     5341 tttgagacct ctagtacttt catagacctg aacaaaagcc tccttaccgt tgacctttat
     5401 tacctctgcc ataagttttg tccctgagag gtcaatatag caaatctcgt tttgagcaac
     5461 gggaccgtca acctctatag taacaagatt ggcaataatc ccggtaactt ttccagtggt
     5521 tttcatataa taatttattc tatattattc ttataagagt ttttcatctc ttctacatat
     5581 ttgcgaaaca gcatttctcc ctgactttta tccatggaac gccatctttt taaaatgaat
     5641 gctctgagaa tatatgagag tataacattc atgtcaaagt agttgaaaac acatattttt
     5701 tctgcttctt cccatcttaa aaggtctaat gctttttctc tctccaatat atctgacatt
     5761 tcaaaaattt gaagaagttt gccggcaaat tcaatttcag atgttattcc aaaatccggt
     5821 gctttatttt ttttcaatgc ttcaacaata tcgttttctc caacaaggta tggtgaaggg
     5881 tcaacattcc ttttacggga aatataggct gcctgaatat ttctaagcat aaggtcaaat
     5941 ccaaaataca gtctgagaaa cttaattttg ttttttgaaa taaccctgta gaaatgtgga
     6001 gacagattgt ctccctcaag accaaacatc agccagtcaa cgtatcttct gtctttatcg
     6061 tcgagagagg agataatctg actgacttga gagtcataat ctatagatat attatcaaag
     6121 tcaggttcta tgtaaggtaa tcctgctatg ataaactcgt agtttttcat agcctctact
     6181 ttgcaaatat aatttcacga attcttggtc taaggtatgt gcctattaac tcctcgaagt
     6241 ccttatctgt gaagcttata taatatcctt cagctttagg gccaatttta aatcctgaag
     6301 aaagcttatg atcaaaccga atctctatgc cacctttaaa ttgttgtgct atgctattta
     6361 caatataatt gtcgagatct cctttcatct tttcaggaag aattatagac aggtcggcag
     6421 gtttgtcact cgtttgggaa aaggcattga ctatagtttg aattaagctc ttaataaaag
     6481 aactgtcact catggcctca gagacaggtt tacctattgc atttgcaata atcatattct
     6541 ctatctcatg tttgagaact gtatagctct gtcttgatga cattttaacc tcatttagaa
     6601 ccctttcctt tatatcttca gcctcctttt gtgctgtggc tataatctta ttggattctt
     6661 catgagcctt tgaaatgata agttctgcct ctttaacagc atctgccttg atcttttcag
     6721 cttcctccct tcccttggaa agtccttcgg agtataattt ctctgtaagc tcttgcaatt
     6781 tgttctgcat agtaaactaa aattagaatt tcaaagttat aaaaaaaaat aactctttat
     6841 tgtcaatatt actctaattt tgaaaaatca caaaaatgtc ttacgaaata aaacaaaaaa
     6901 ggagcgtttg acacagtaat ttgtttcgaa acgctccaag tatggttttg tttaagaact
     6961 atccaagaaa tccgagtaat ataccggcag caacagcact tcctataact cctgcaacat
     7021 ttggtcccat ggcatgcata agaaggtgat ttgtcttgtc atattcctga cctattactt
     7081 gtgaaacacg ggcagcatca gggacagctg atacaccggc attcccaatg agggggttga
     7141 ttttgttacc ttccttgagg aacagattga aaaatttcac aaaaagaact ccacctgtag
     7201 tggctattat aaaagataat gcaccaagaa taaaaatata gatagaggtc aactgcaaaa
     7261 atttatcggc ttgtgtggat gctccaacag ttagaccaat aaggatagta ataacatcaa
     7321 tcagtggtcc tcttgcagtc tctgccaaac gacgtgtaac tccgctttct ttaagtagat
     7381 tgccaaaaaa cagcatgccg agcaatggaa gaccagaagg tactatgaaa caggtgagaa
     7441 gaaaaccgat aattggaaat agtattttct ctgtttgaga aacagccctt ggtggcttca
     7501 tccttatcaa acgctctttc ttgtttgtga gaagtctcat gacaggaggc tgaattactg
     7561 gtacgagtgc catatatgag taagcagata cggcaattgc tcccaaaaga tttggagcaa
     7621 gtcgtgatga gaggaaaatg gcagtaggac catcagcacc accaattatt ccaatagcac
     7681 cggcatctga agccgaaaaa cccataagca gtgcaatggc atatgcacca aaaataccca
     7741 attgtgcagc tgcaccaatg agaagtaatt ttggattgga aattaaagat gaaaaatcag
     7801 tcattgcgcc aatgcccaag aaaatgagag gaggatacca gccttggata acaccctgat
     7861 aaagtatgtt aagcactgag ccctgttcat acacgcctat gcttaagcct ggaaaaaaag
     7921 gaatattacc tacaatgatc ccaaatccaa ttggtaccaa caacattggt tcccattgtt
     7981 ttttgattgc aaggtaaata aacgcaagac ctataacaat cattattaga tgattgaagg
     8041 tgacattggc aaatccggta tattggataa attgacgtaa attttctcca atcaatgaaa
     8101 gtgaaccatt ttccatatta tccaatagtt actaatgttg ctccctctag aactgaatct
     8161 ccctttgaaa ctttaacaga tatgattttc ccatcagcac ttgcttcaat aacattttcc
     8221 attttcatcg cttcaagaac aatcagtttc tgcccctttt taacaatatc accttctttg
     8281 actgcaactt ccagaataat tccgggaaga ggagatgtaa cattaattac tgttgaagaa
     8341 gaaggaggag tcacttgaac agaaggtttt tgaactgtgg aagatggtga aactacagaa
     8401 gataaggttt taggaggagt gactatattt tttttaatcg gattttcaaa ctcaacttta
     8461 aaaggtatgc cgtttacctc tacttcggca agattctcct cgatatcgga aacagtcact
     8521 gagtaggcat ttccattaat ttttaattta tattctttca tatcttttta aaatttttca
     8581 ttttttttga ggtgttttac gaagtgtata aattttagaa ctccaaggtg aataattttt
     8641 tgtaaccctt gaaattgtaa gtactgtatt ttcaacatcg tgggtttcat tgcctcgaag
     8701 gtaagaatga agcgctgctg cgattgctgc ataaatctcg gctgagacat tagtggcttt
     8761 tggcttgtca ctatcactaa gtgataccaa agatctgtca cgacttttct taatgtgata
     8821 attgccaact tgtttgaaaa tgatgtaaag aagaataaga gcaataaaaa ccacagacat
     8881 ggctgtgata gacattatta tgccataagg atcagctttc ttcaccaaaa cacttttagg
     8941 tacgtcatct acacctgaat tgtttgtact tggtgatgtt cttttcataa cctgccagcc
     9001 ttctgatccg tcggtcgaac caactccgtc atatgttctt ccataagaaa cattagtgtc
     9061 aagtagatca tgaggtatct ttactcgatc gattattgtt tttccatctg aggcaacaaa
     9121 cagaatctct tgagaatctt caagtttgaa attgatatgg aatgtgcccc taaaaggttc
     9181 attatcagcc cataagagca cgtgttgtct aggttgaatt tttgtgagaa cgtcaccttt
     9241 tgggattatg tattttgtga gatttgaagg atcatttgtc aaatagcatc caccaatatt
     9301 gacagtgccg taggaagaat tgaagagttc tatccatcca tttttatgtc caaaatcatc
     9361 ttcaaaatca ttcgtattgt gcacaagaaa ttcatttata cggatgtcat tctggctttg
     9421 cgctccaagt gtcaaagtga tagacataaa tccaagcaac agaaatactt ttgatattat
     9481 tctcatattg tgctgatttg ttaattagag tggaagatta tcatgcttct ttggaggatt
     9541 ggtcaatttt ttcgttgtca actgttgaag agcacggata atcctgaagc gggtattacg
     9601 tggttctatg acgtcatcta tgtaaccata gcgtgctgca ttgtaagggt tagcaaatgc
     9661 ttctctatat tctgcctctt tttctgcggc aaatttctcg ggattttctg ctgttgccat
     9721 ttccttgccg tacaatacct cgatagctcc tgaaggaccc ataactgcaa tttcggcaga
     9781 tggccatgca taatttatat cacctctcaa atgcttgctg ctcattacac aatatgcgcc
     9841 cccatacgat tttctcaaag taacagtgat ttttggaact gttgcctctc catatgcata
     9901 aagcaattta gctccatgaa gaataatgcc accatattcc tgttgagtgc ctggaagaaa
     9961 tccaggcaca tctacaagtg taacaagtgg aatattaaat gcatcgcaaa agcgaacaaa
    10021 acgtgctgcc tttcgggatg cattaatgtc aagtacaccg gcaagaactt taggctgatt
    10081 tgccacaata ccgacagatg cgccgttaaa tcttgcaaaa ccaactatta tattagttgc
    10141 atagtcctta tgaacttcga ggaacttgcc attatctaca atagatccaa tgacttcata
    10201 catatcgtat ggtttgttgg ggctgtcagg aattatttca ttcaatgaat cttcaagtct
    10261 gtctatagga tcagtatttt cagtcatagg tggatcctca agattatttt gtggcagata
    10321 gccaagtaaa tcacgaataa cttgaattcc ctcttcttcg ctgttcactg caaaatgtgc
    10381 aacaccgctt ttgctcgagt gaacagatgc acctccaagc tcttcttgag tgacatcttc
    10441 acctgtaaca gttttgacaa ctttaggacc tgtaaggaac atgtaacttg tgccttttgt
    10501 cattatattg aagtcagtca gagccggtga gtaaactgct cctccggcac atggaccaaa
    10561 aatagcagaa atctgaggaa tgacgcctga agccagaata tttcgctgga aaatttcggc
    10621 ataaccggca agagcattga ccccttcctg gattcttgct cctcctgaat cgttaagccc
    10681 gataacaggt gcccccatct tcattgcctg atccataatt ttgcagatct tcattgcaaa
    10741 actttctgaa agtgatcccc caaaaacagt aaaatcttgg gcaaaaacgt aaaccagcct
    10801 tccatctata gtcccaaatc ctgttacaac tccatctccc aggaatctct ctttttccat
    10861 tccgaaattg gtgcaccggt gtgttacaaa catatcaaac tcctcgaaac ttccttcgtc
    10921 gagcagcatt gctattcttt cacgagcggt aaatttccct tttgagtgtt gtgagtcgat
    10981 ccttttttga ccacctccca gacgcgcctt ttcacgtaat tgtgtcagcg ctttaacttg
    11041 ttcaagttgt tggctcatta tatttcttat tagatgatta ttctctcatt tggactttga
    11101 gggatcttca cacaattctg tcaaaactcc gaaagttgat ttgggatgaa gaaaagctat
    11161 tttaagattt tctgcaccat tgcgaggaga tttgtcaatc agtcttattc cttttgtttc
    11221 caattcatta agagaatttt gaacaccatc ttctactgca aatgcaatat gatgaactcc
    11281 ctcacctcta ctttcaatga attttcctac tgctccttca ggatccgtac tttcaagcag
    11341 ttcaatctta gtttctccaa ttttaaaaaa agctgtttta actttttgat ctttaacctc
    11401 ttctacggca taacatttca agccaagcac tttttcgaaa taaggtattg cagtgtcaag
    11461 agatttaaca gcaataccaa tgtgctctat atgagacaat ttcataaaat tttattgttt
    11521 aatattttta gactgccatt gtgtgatata cattctgaac atcatcatcg ttttcaaatc
    11581 tttctatcaa cttttctatc tctgctttct gatcttcatt taactccttc aattcggtgt
    11641 tggggattct ttcaaaacca cctgaaacca gttcatagcc accctcttct atgtattttt
    11701 gaatggcacc aaaagcctca aaagcgccat aaatgacaat attgtcgtct tccataaaca
    11761 tttcttctcc accatagtct ataagttcaa gctcaagctc ttccaagtta acaggttttg
    11821 aacttctaat gcgaaaaaca cattttttat caaacataaa atctacgctt ccctgtgttc
    11881 caagagatcc gttgaatttg ttgaaatagc ttcttatatt ggccacagtt ctcgttggat
    11941 tgtcagtggc agtctctata aggaaagcaa ttccgtgggg accatatcct tcatagacaa
    12001 cttccttata ttcactatgt tctttttcaa cagctctttt gatagctctt tcaatatttt
    12061 ctttgggcat attttctgag cgggcagtcg agataatagc tctaagttta ggattgttgt
    12121 caggatcagg tccacctgat ttggcagcaa ttgtgatctc cttacccagt ctggtaaaag
    12181 tgcgggacat atttccccac ctcttgaatt ttctctcttt tctgaattca aatgcccttc
    12241 ccatatatag gaatcatatt atatttgcta cttcttaatt ctataaatat atttatcaat
    12301 gtcatatcct tcaatagtgt taggatattt agccttgatc tcttcgtaag ttttaagagc
    12361 gttgtcaata tctccaagtt cctcatacat tattcctgcc ttgagcagat agcctgctgc
    12421 atagtcgtta tcagccaatt tagcggcctt gttgtagtag ttgagagctt ccttgttgtt
    12481 ttccaggaaa gcatatgcat ctccaatgtt gcaaagtgcc ctggcttgaa gaattggatc
    12541 ttttcctttg tattttttaa ggtattcaat agcttctttg tattgaccaa gctggagttc
    12601 acatatcccg gcatacagat aaactgcagc gccggatttg tctccgtatt cttcaattat
    12661 ctgtttaaaa ccaagtgcat taccatcacc attaaaggcc aatgcaaaag aatctgcacg
    12721 gaaataacgt tcggccacaa atgattgtgc catagcctca ttggtcttag gtttgcggta
    12781 aaattggttg tatcctaaaa tgatagctgt tatagctaat attgtaatta cggaatacaa
    12841 taaaggtttc ttgaatcttt cgataaaatg ttcagtttga tttacaactt cagaaactgc
    12901 ctcttgttcg ttttttttct tcttagacat ataatttttc ttttatgcag tctgcaaaat
    12961 tacaataatt tgtctaaaca caaatattta ttaattgaaa aaggagtaac tttgcttgta
    13021 aacagaatga ttgaaaaatg tatctaaaaa aaatcacgat caataatttt aaaaatattt
    13081 cagaagcaca aattatctta tctcccaaga ttaattgcat tacaggaata aatggctctg
    13141 gtaaaacaaa tctacttgat gctgtgtatt atctttcaat gaccaaaagt tttttttctt
    13201 ctatagatca tttctcaatt agatataata ctgattctgc gacaattaat ggagagtatg
    13261 ttagagagaa caataatgga gtagatatta tttctctttc atttaatata aatggtgaga
    13321 agcacttaaa aagaaattcc cgaagttata gccgtttttc agatcatatc ggacttttac
    13381 caattgtaat ggtctcgccg gctgacacat ctcttgtaaa tgagagtgga gacgagagac
    13441 gtaaatttat gaatgccatt ctttctcaaa tagataaaga atatttacga cgtcttcaaa
    13501 attataatca gcttcttgcc cagaggaaca gattgctcaa aggtgatgtt gtctctgacc
    13561 tgcttctgtc gactttaaat gagcaaatgt ctgttaatgc agagtatgtt tatttaaaaa
    13621 gaaaagattt tataaataaa atggtcccgg ttattaagga ttattatgat atgctttcag
    13681 gtgacagaga agaggtgaat attgaatata aatccgatct tcatacctgt tcgcttgacg
    13741 aacttttttc aagaaatttg gagaaggata aatttcttgg gtatacttca tctgggattc
    13801 acagagacga tttaaatttc acaatggatg gatatccaat aaggagaggc gggtctcagg
    13861 ggcagcagaa aagctttctt attgcactta aacttgctca gtttacagtt atgaagaata
    13921 ttcactgtg
//
LOCUS       DAOTQL010000033        13823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4192_length_13823_cov_4.869407, whole genome shotgun sequence.
ACCESSION   DAOTQL010000033 DAOTQL010000000
VERSION     DAOTQL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13823
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_13823_cov_4.869407"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="P5293_03340"
     CDS             <1..1371
                     /locus_tag="P5293_03340"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRR48967.1"
                     /translation="ATLDNKINFKSEEFGEADFMQKFKAGVKHNFTIGLPTFTLFKYI
                     QCSPGVSYGMNWYFQSMDRSYDPSTDQVVNDTSALFKHFGATQDYSGSLSINTRLYGM
                     YNFGDRGKIRAIRHMITPQISLSYRPDLGTYGNGYRTLNYTDKDGVLKTMRYNIYDGL
                     PYGYPSSGESGGLSFSVANNVEAKVKSDKDTTNGGITKIKLIDNLSFAGSYNFLADSM
                     RLSNISATMSTTIFGSLAFNANAMFDPYAIDEKGQRINELNIVKEGGFKLARMTNASV
                     SLSYSLSGKGERGGGQSEANKVQSPATTVGGHPNPVYYNRVYKHPLTGEYIPGGWLYY
                     LDPSIPWSLSFNYNYSYSKSYSYAGGQLSKINSFTQTLGISGQLRMTKDLNVSLNTGF
                     DLMERKLTTTQMSATYDLHCFQISFSWVPTGTWKSWSFRINAKASALADLLQYKKSAS
                     YWDNKH"
     gene            1386..1763
                     /locus_tag="P5293_03345"
     CDS             1386..1763
                     /locus_tag="P5293_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRR48968.1"
                     /translation="MKNVISSVRAPKAVGPYSQATESNGFIFISGQLPIDVSKGEIVD
                     GGIREQTAQSLRNIGYILEEAGCTFDNVLKSTVYLSDINDFTAMNEVYATFFKEPYPA
                     RVAYAVVALPKGALVEIDVIAAK"
     gene            1864..3843
                     /gene="rho"
                     /locus_tag="P5293_03350"
     CDS             1864..3843
                     /gene="rho"
                     /locus_tag="P5293_03350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRR48969.1"
                     /translation="MYDIKELSKMSQEDLLAIAQKLNVSKAKSFSTEELIYEILDEQA
                     KQGSLTNEPLKKPLVQRQKKQRINKPKEGQKVGVSSKVEESSAEASKLELHKNEDLRA
                     AAPSDKTVPSSSSNTEISNTETANKETSSSSKKRGRKPTVVKVLEQPKAKQETEQKNQ
                     VKTESEIVISEKSQKETQSAPQPSQHPPQAQGQAQAQHVQQYQNKQQNQYPQQTGRVQ
                     HNQQQQQNGQQQQNGQPHQNSHPQQQAQQQHKQHHNLSKDNKMSQPSDDELSIESVEN
                     SIKEQYKKVEFEGTVEATGVLEIMPDGYGFLRSSDYNYLNSPDDIYISQSQIKLYGLK
                     TGDTLVGDIRPPREGDKYFPLVKVNQINGRSPEFIRDRIPFDYLTPLFPNEKFDITSN
                     GHNNLSTRIVDLFAPIGKGQRGLIVAQPKTGKTILLQDIANAIAANHPEVYMIVLLID
                     ERPEEVTDMARSVKAEVIASTFDEPAERHVKIANIVLEKAKRLVECGHDVVILLDSIT
                     RLARAFNTVQPASGKVLSGGVDANALHKPKRFFGAARNIENGGSLTILATALIDTGSK
                     MDEVIFEEFKGTGNMELQLDRKLANKRIFPAVDVTLSSTRRDDLLFDKDTLNRIWILR
                     NYLADMNSVEAMEFMKTRLMRTENNQEFLMSMNGE"
     gene            complement(3844..4956)
                     /locus_tag="P5293_03355"
     CDS             complement(3844..4956)
                     /locus_tag="P5293_03355"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HRR48970.1"
                     /translation="MNLVNIIIEALKNSILITGLVMVMMLLIEYVNVHSHGSTFNKLR
                     NSPVKQVLLSALLGLVPGCVGGFAVVSLYTHNIVSFGSLLAMMISSSGDEAFVLLAAV
                     PKTALILFFTLFITGLLIGIVADKVFKDRRRPFAPEHYKIHENEYFVSSSPIFGNSIR
                     ENLKKLSKERLIIVVGVAIFVIAVVMGILEHDHIETGASGETHSTDEHLHGSINIFSE
                     RWINLLFAGISIITLYLTLKAKEHFIKEHLWNHVIVNHFKSIFLWTFGALIVIHFGTQ
                     YLHIENWVRENVIIMILMAAAIGLIPESGPHMIFITLFANGLIPFSVLFVSSMVQDGH
                     TALPLLAESKGSFFKAKAINFVAGITIGITMHLFGI"
     gene            complement(4953..6035)
                     /gene="meaB"
                     /locus_tag="P5293_03360"
     CDS             complement(4953..6035)
                     /gene="meaB"
                     /locus_tag="P5293_03360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRR48971.1"
                     /translation="MNDIERDSALIVNEGVEQPPVVNPYINNFFKKNRSRNLTVKDYV
                     EGVLAGNTTVLSQAITLIESSKPEHRIVAQQVVEACQPFAVQSMRIGITGVPGVGKST
                     FIEAFGGLITSRGHKLAVLAIDPSSERSKGSILGDKTRMETLSSDPNAFIRPSPSGGS
                     LGGVARKTRETIVLCEAAGYDVIFIETVGVGQSETTVHSMSDFFLLLMLAGAGDELQG
                     IKRGIMEMSDLIAITKADGSNIQKAEMAKALYSNALRLFPPTESGWIPTALTTSAVNK
                     TGLDEILSKIFEYFQLTRENGYYIHKRREQAKFWMYESINDELKNRFYEDPKIQVKLK
                     EYEELVLSGKMGSFTAASELLKLYNK"
     gene            complement(6032..7132)
                     /locus_tag="P5293_03365"
     CDS             complement(6032..7132)
                     /locus_tag="P5293_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRR48972.1"
                     /translation="MKNILYKMCAMTFITLMSITLLLAQTTPQMSIEFNKTIHDFGYI
                     DMNSGPRSCSFIFKNISNQPVVIQTVISSCGCTTPEWTKSPVKPGEKGKIDVTFLNDQ
                     GPYPFDKSLTVYVTGSSRPIVLRVKGVVTQKPKTLRQQYPVKIGQLGLKRNPVELGQI
                     QYGTISKGAVEVVNLAGNPIKISFTDFSKGFYINAVPDLVPENGKTDLIFSIDTKAAN
                     NWGTTIFSASPVINGKKADRPIQIKATILENFADMSKAELDKAPLPIFRRSSFSFGEV
                     NEGSTVKCSFDFRNTGKSDLIIRQIQYSEDGIIAKYKDKIPASGSSKIEIEINTKNMS
                     GEKNFVVTLVTNSPTRPIINLIISGKVTNKNS"
     gene            7237..7404
                     /locus_tag="P5293_03370"
     CDS             7237..7404
                     /locus_tag="P5293_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR48973.1"
                     /translation="MAYKISEDCTACGTCIDECPVEAISPGDIYKIDPNICSDCGTCA
                     DVCPVGAISQM"
     gene            7521..8792
                     /gene="serS"
                     /locus_tag="P5293_03375"
     CDS             7521..8792
                     /gene="serS"
                     /locus_tag="P5293_03375"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRR48974.1"
                     /translation="MLNIKLLRERPQFVAERLAVKNFDATEIIKSINELDQKRRETQT
                     ELDTILSEQNLLSKKIGLLMKDGKIVEAEEVKAKVSDLKEKSKLLENEMQIVSSKMNE
                     QLLLLPNLPHISVPSGKSAEDNVIVREGGSIPEFDKSALPHWELARKYDIIDFDLGVK
                     LTGAGFPVYKGKGAKIQRALISYFLDKNTNAGYTEFQPPLMVNEASGYGTGQLPDKGE
                     QMYYVNLDNFYLIPTAEVPVTNIFRDVILGYSDLPVKCTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQITHPDNSYDALEEMVKHVEGIVRSLCLPYRILRLCGGDMSFTSA
                     LTYDFEVYSAAQRRWLEVSSVSNFETFQSNRLKLRFKDENGKTQLAHTLNGSSLALPR
                     ILAALLENNQTERGIEIPSVLREYTGFSYID"
     gene            8798..9352
                     /gene="ruvC"
                     /locus_tag="P5293_03380"
     CDS             8798..9352
                     /gene="ruvC"
                     /locus_tag="P5293_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRR48975.1"
                     /translation="MDDEKIILGIDPGTRILGWGVVRSFGNSVDFLDMGVVDMHSETD
                     HYIKLGIIFREVSALINRFNPTDLSIEAPFYGKNVKSMFLLGRAQGAAITAALALGIP
                     VSEYAPRKIKMSITGRGAASKEQVANLLYNILKIEQSVNYFDATDALAVAYCHFLETS
                     NPYFGKGFNESWKSFVKRNSSRVK"
     gene            9479..12295
                     /locus_tag="P5293_03385"
     CDS             9479..12295
                     /locus_tag="P5293_03385"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRR48976.1"
                     /translation="MIFLNSTKTFAVDVEPKDFRIKIILIDSVTQDPIEFATVSITPE
                     GKKEALKYALTDASGWAEIVKVPEGTYTLKAECLGYLPFETKVNTTGKSLLDLGRVKM
                     KQEINVLSTVTVTALGNPIIVKKDTIEYNASSFKVTDQDMLEALLKKLPGIEVSSDGT
                     ITANGKTIDKITIDGKTFFFNDPQLATKNLPAKIIDKVKVLEKKSEQAQFTGIDDGQS
                     ETVIDLSIRPGMMNGWFGNVTGGYGTDDKYQVAGMLGNFTSKSQISMIANANNTNNRG
                     FFDVAGDAMRSMRNSLGGAGGVRIGGAVMNFGGSGITTSWMGGVNANTVSNNKKLNIG
                     GSYLYSGSDTENKQVTSKQTFLADSSFIYNSNQYSKSSTDANSASVNLEYKFNDKTSI
                     LFRPNMNIGSGSFNEENVYFTKGVLGSSINDGSSKSYGNSDTRSLNGDLLLRQKFNKT
                     GRTFSVNFNYGYSNNDIEAFNESTTNSYGTNPLTTEVRQKYNTEQDNYSLGGRASYTE
                     PLGNHYYMELAYSYNFRKNNSDKVAKNFNQNTGNYDLYDSTYSNSFENTFINQIGELN
                     IRKVEEKYSFTLGMNVQPAYTHSITTSDLKTTDLERSVVNYSPSALLDIDFSDSKSLR
                     IRYRGSTRQPSISQMQPVKDNSNPLFVSEGNPDLLPEFSHNLSLNFRNTNRSNFSTVN
                     ARLDGTYTLDKIANKTWYEEGGIQHTKPVNDQAVYSISGNIMWNTPIEKTKKFYFMTN
                     TRAGINNGINYSSSTASETGAVKNITTSLSVSEMLRLAYRGEKLEITAGGRVSYAKAW
                     YTIGETKDATWNNSLNATINWTLPAGFGLASDIEHQFYVGYGEGYNDPATVWNAEVSK
                     LLFKNSASLIFRVFDILDQSKSVRRTTTDNYIQDVSSLTLKQYFMLSFTWRFGKFGDR
                     SGQQGGSRGMNHGRFGPPGGVFHR"
     gene            complement(12301..>12689)
                     /locus_tag="P5293_03390"
     CDS             complement(12301..>12689)
                     /locus_tag="P5293_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRR48977.1"
                     /translation="KVFTDGSIGKHAITHYKVIERFGYTTLVSCRLETGRTHQIRVHM
                     DYIGHPLFNDDRYGGDKIVNGTFYAKYKQFVDNCFELLPRQALHARTIGFTHPKTGER
                     LFFESPVPSDMLSVIEKWRSYSHYKR"
     assembly_gap    12690..12699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12700..13339)
                     /locus_tag="P5293_03395"
                     /pseudo
     CDS             complement(12700..13339)
                     /locus_tag="P5293_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655702.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
     gene            complement(13341..>13823)
                     /locus_tag="P5293_03400"
     CDS             complement(13341..>13823)
                     /locus_tag="P5293_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRR48978.1"
                     /translation="NTIQPKLVTMPSVTGFSLRQALAELEAKQLSVGRLTYVYDMATD
                     NVLSQKFNGRFIAPGTKIEAGSQIDLELGDNGQSATSIPDVTGVSLQLAKEILLENSL
                     NLGKIHYDNLVKSYSDTVSSVVIRQIPSPTRVTSYPLGTEVELFLSINKEKSKAIKTE
                     "
BASE COUNT         4291 a         2452 c         2857 g         4213 t
ORIGIN      
        1 gcaacccttg acaacaaaat taattttaag tcagaagagt ttggagaagc agattttatg
       61 caaaagttca aggcaggggt taaacataat ttcactattg gtcttccaac gtttacactt
      121 tttaaatata ttcaatgttc tccaggggtc tcgtatggga tgaactggta ttttcaatca
      181 atggatcgat catatgaccc ttctaccgat caggtggtta atgacacatc tgcactgttt
      241 aaacattttg gtgcaactca ggactattca ggttcgcttt caattaacac tcgactgtat
      301 ggaatgtata attttggtga cagaggaaag atcagggcta taaggcacat gataacgcct
      361 cagatatcac ttagttacag gcctgatctc gggacttatg gcaatggata caggactctc
      421 aattatacag acaaagacgg agttttgaaa acgatgcgat ataatatcta tgacggtctt
      481 ccatatggct atccatcttc aggagagagc ggcggattga gtttctcggt agcaaacaat
      541 gttgaggcta aggttaaaag cgacaaggac accacaaatg ggggcatcac aaaaattaaa
      601 ttgatagaca atttatcttt tgccggttca tacaattttt tggcggattc tatgagactt
      661 tcaaatattt ctgctactat gagtactact atttttggct cattggcatt taatgcaaat
      721 gcaatgtttg atccttatgc gattgatgag aagggccaaa ggataaatga attgaatatt
      781 gttaaagagg gcggttttaa attggcgaga atgacgaatg caagtgtatc actttcctat
      841 tcgctatctg gcaaaggaga aagaggagga ggccagtctg aagccaataa ggtacaaagt
      901 cctgccacaa ctgtaggagg acatccgaac ccggtctatt ataatagggt atataaacat
      961 cctttgaccg gagagtacat tcctggagga tggctctatt atcttgatcc ttcaattcct
     1021 tggtctttga gctttaatta caactattct tacagtaaat catattctta tgccggaggg
     1081 caactttcaa agataaattc ctttactcaa actcttggca tttcaggtca attgaggatg
     1141 acaaaggact tgaatgtttc attaaataca ggttttgatt taatggaaag aaaactaacg
     1201 accactcaga tgagtgctac atatgatctt cactgtttcc aaatttcttt ttcatgggtg
     1261 ccgaccggta cttggaagag ctggagcttt agaataaatg caaaggcttc ggcacttgca
     1321 gatctgctac agtacaaaaa aagtgcaagt tattgggata ataaacatta attagatata
     1381 taattatgaa aaatgtaatt tcatctgtta gagcgccaaa ggctgttgga ccgtacagcc
     1441 aggcaacaga gtcgaatgga tttattttta tttcaggtca gcttccgatt gatgtctcta
     1501 aaggagagat cgtggacgga ggtataagag aacaaactgc ccagtcactt agaaatattg
     1561 gatatatttt ggaagaggca ggatgtactt ttgataatgt actcaaatca acagtgtatc
     1621 tttctgatat aaatgacttt acagcaatga atgaggttta tgccactttt tttaaggaac
     1681 catatcctgc aagagttgca tatgctgtag ttgcattgcc taaaggtgca ctggttgaaa
     1741 tagatgtgat tgctgcaaaa taaagttaat tttggatatt cattatatgc tttcaatatt
     1801 tttactaatt ttgcaacaac caattattct ttccaggtta ataattaccc ccttatacat
     1861 ctaatgtacg atattaaaga gttaagcaag atgagtcagg aagacttgct tgctattgcg
     1921 cagaagctga atgttagcaa agccaaaagc ttttccactg aagaactgat ttacgaaata
     1981 cttgatgaac aggcaaaaca gggcagtcta acaaatgaac ctcttaaaaa gccattagtc
     2041 caaaggcaga agaagcagag aatcaacaaa cctaaggaag ggcaaaaagt gggagtgtct
     2101 tctaaagtgg aagaatcttc ggctgaggca tcaaaattag agcttcacaa gaatgaggat
     2161 ttacgagcag cagccccttc tgataaaaca gttccttctt caagctcaaa tacagaaatt
     2221 tctaatactg agactgcaaa taaggaaact tcttcttcat ctaaaaaaag aggcaggaaa
     2281 ccaactgtgg ttaaggttct tgaacagcca aaagcaaaac aggaaactga gcagaaaaat
     2341 caggttaaaa cagaaagtga aatagttata agtgaaaagt ctcaaaagga gactcaatct
     2401 gctcctcagc cttcacaaca ccctccacag gcacagggac aggcacaggc acagcatgtg
     2461 cagcaatatc aaaacaaaca gcaaaatcag tatcctcagc aaaccggtcg ggttcaacat
     2521 aatcagcaac agcagcagaa tggtcaacag cagcagaatg gtcaaccaca tcagaatagc
     2581 catccacagc aacaggctca gcagcagcac aagcaacacc acaatctttc gaaagataat
     2641 aagatgtctc agccaagtga tgatgagttg tcgattgaaa gcgttgaaaa tagcataaag
     2701 gaacagtata aaaaggttga atttgaaggt acagttgagg ccacaggagt ccttgaaata
     2761 atgcctgacg ggtatgggtt tttgaggtct tcagactata attacctcaa ctcgccagat
     2821 gatatttaca tatcccaatc acaaattaaa ctttatggct taaagactgg agacacactt
     2881 gttggggata tcaggcctcc aagggaaggc gataaatatt tccctcttgt taaggtaaat
     2941 cagataaacg gtcgttctcc cgaattcata agagatcgta tcccttttga ttatcttact
     3001 cctcttttcc caaatgaaaa atttgatatt acttccaatg gtcacaataa tctttccaca
     3061 agaattgtag atctctttgc gcctataggt aagggtcaga gaggtttgat agttgctcag
     3121 ccaaagactg ggaagactat tcttctccag gatatagcca atgcaattgc tgccaatcat
     3181 ccggaggtat acatgattgt tcttcttata gatgaacgtc cggaagaggt tacagacatg
     3241 gcgcgaagtg ttaaggctga agtaattgca tctacttttg acgaaccggc agagagacat
     3301 gttaaaattg cgaatatcgt tcttgagaaa gctaaaagac ttgttgagtg tggtcacgac
     3361 gtggtgattt tactggactc catcacacgt ctggcgagag ccttcaacac tgtgcagccg
     3421 gcttcaggta aagtgttgag tggaggtgtt gatgctaatg ctcttcataa acctaaacgt
     3481 tttttcggtg cagccaggaa tatcgaaaat ggcggttcac ttacaattct tgctacggct
     3541 ctgattgata caggttccaa gatggacgag gtgatatttg aagagtttaa gggtacaggt
     3601 aatatggaac tgcaactcga caggaaactt gcaaataaaa gaatatttcc tgcagtcgat
     3661 gttactctta gtagtactcg tagagatgat cttctttttg acaaggacac tttaaacagg
     3721 atttggatct tgagaaatta tcttgctgac atgaattcag tcgaggcaat ggaattcatg
     3781 aagaccaggc ttatgcgtac agaaaataat caggagtttt tgatgtcaat gaatggagag
     3841 taactaaatt ccgaaaaggt gcatagttat tccaatagtt attcctgcaa caaaattgat
     3901 ggcttttgcc ttaaagaagc ttcctttgct ttcagcaagt aatggaagtg cggtatgtcc
     3961 gtcctgcacc attgaactaa caaataggac actgaaagga atgagaccat tagcaaagag
     4021 tgtaatgaat atcatatgtg gtccagattc aggaataagt ccaatagctg ctgccatcag
     4081 aatcattatt atgacattct ctctgaccca attttctatg tgaaggtatt gagtaccaaa
     4141 atgaatgaca attaatgctc cgaaagtcca gaggaaaata gatttgaaat ggtttactat
     4201 tacatgattc caaagatgct ctttgataaa atgttctttg gctttgagtg tgagataaag
     4261 tgtaataata cttattcctg caaacaggag atttatccat ctttcactga aaatattaat
     4321 tgagccatgc agatgttcat ctgtagagtg agtctctccg gaagcaccgg tttcaatatg
     4381 atcatgctcc aaaatgccca tcacgactgc aatgacaaat atagctacac caactacaat
     4441 aatcagtctt tccttactta attttttaag attttctcta atactgttgc caaatattgg
     4501 agaagaagag acaaagtatt cgttttcatg gatcttgtag tgttccggtg cgaatgggcg
     4561 acgtctatcc ttgaaaactt tatcagcgac aatgccaata aggagacctg tgataaaaag
     4621 tgtaaaaaac aaaatcaggg cagtttttgg aacggctgcc agaagaacaa atgcttcgtc
     4681 tcctgatgag gagatcatca tagcgagtag tgaaccgaag ctaactatat tgtgtgtata
     4741 gagagagacg acggcaaaac ctcctacaca tcccggaacc aatcccagga gtgctgacag
     4801 aagaacctgt tttacaggag aatttctaag tttgttgaat gtggatccgt gagagtgaac
     4861 gttgacgtat tcaatcagaa gcatcattac catgacgagt ccggtaataa gtatgctgtt
     4921 tttgagggct tcaattataa tgtttacaag attcatttgt tatacagttt aagaagttct
     4981 gaggctgctg tgaaagatcc cattttgcct gataaaacaa gttcttcgta ctctttaagt
     5041 ttaacttgta ttttaggatc ttcgtagaaa cggtttttca gctcgtcatt gatggattca
     5101 tacatccaga atttagcctg ttctcttctt ttatgaatat aataaccatt ttctcgagta
     5161 agttggaaat attcaaatat cttggataga atttcatcca gccctgtttt gttgacagct
     5221 gaagtggtaa gggctgtggg tatccatccg gattctgtag gagggaaaag gcgcagtgcg
     5281 ttggaataca atgcttttgc catctcggcc ttttgtatat tagagccatc ggctttagtt
     5341 attgctatga ggtctgacat ttccataatg cctcttttaa tgccttgaag ctcatcgcct
     5401 gcacctgcta acattaaaag aagaaaaaag tcactcattg aatgtactgt ggtttcagac
     5461 tgcccgactc caactgtttc aataaaaata acatcataac cagctgcttc acacagaaca
     5521 attgtctcgc gggtctttct tgcaacgcct ccaagagaac ctccgcttgg acttggtcta
     5581 ataaatgcat tgggatcgga ggagagtgtc tccattcttg ttttgtctcc aagaatactt
     5641 cctttgcttc tttcactgct cggatctatt gcaagtacag caagcttgtg accacgtgag
     5701 gtgattaatc ctccaaaggc ttcgatgaat gtacttttgc caactccggg cactccggta
     5761 atacctatac gcattgactg aactgcaaaa ggttgacagg cttctacaac ttgctgggct
     5821 acaattctgt gctctggttt agagctctcg ataagtgtaa tggcctgact taacacagtc
     5881 gtatttccgg ctaaaactcc ctctacataa tctttaaccg taaggtttct gctcctgttt
     5941 ttcttgaaga aattattgat ataagggttt acaactggtg gctgttcaac accttcattt
     6001 actataagtg cgctgtctct ttcgatgtca ttcatgagtt tttatttgtc acctttccgg
     6061 aaattatcag attaattatt ggtctggttg gcgaattagt tacaagggta acaacaaaat
     6121 ttttttctcc ggacatattt tttgtattta tttcaatttc aattttcgag ctgcctgagg
     6181 caggaatctt atctttatat tttgctataa taccatcttc actatattga atttgtctga
     6241 taattaaatc gcttttcccg gtatttctaa aatcaaaact acattttact gtagaccctt
     6301 cgttcacctc tccaaatgaa aaggaacttc tcctaaaaat tggcagtggg gctttgtcaa
     6361 gttctgcttt tgacatatcg gcgaaatttt caagaatagt tgctttaatc tgaattggtc
     6421 tatctgcttt tttaccatta atgacaggac ttgctgagaa aatggttgta ccccaattgt
     6481 ttgctgcctt tgtgtcaata gaaaatatca agtcagtttt tccattttca ggcactaaat
     6541 caggaacagc atttatataa aatccttttg agaaatcggt aaaacttatt tttatcggat
     6601 ttcctgcaag gttaactacc tcaacagctc cttttgaaat agtaccgtac tgaatttgtc
     6661 ccagttctac aggatttctt tttaatccaa gttgtccaat cttgactgga tactgctgcc
     6721 gaagagtttt tggtttctga gtgacaacac cttttactct taacacaata ggcctggatg
     6781 atcctgttac atatacagtt aacgatttgt caaatgggta aggtccctgg tcgtttagaa
     6841 aagtaacatc aattttccct ttttcacctg gtttaacagg acttttggtc cactcgggag
     6901 tggtacatcc acatgaggaa ataacggtct gtatgactac aggctgatta ctgatatttt
     6961 tgaaaataaa ggaacaactt cttggacctg aattcatgtc gatatatcca aaatcgtgaa
     7021 tcgtcttgtt gaattcgata ctcatctgag gtgtggtttg tgcaagtaag agtgtaattg
     7081 acatcaaagt gatgaacgtc atggcacaca tcttatataa aatatttttc atcacaatta
     7141 ggtttatgtt tcagtccaca aagatacaat tactatttgt tcttttctac ccattttata
     7201 cctttgcaga taataaacca attaaatttt atagtaatgg cttacaaaat ttcagaagac
     7261 tgcacagctt gtggaacatg tatcgatgaa tgccctgtag aggctatttc acctggtgat
     7321 atctacaaaa tagacccaaa tatctgttca gactgtggta cttgcgctga cgtttgccct
     7381 gtaggggcta tcagtcagat gtaatttatc ttgctgtaca acaaaatgag ggggtgtcat
     7441 ttattggcac tccctcttct attagttata tgttagagag taattttttc atacctttga
     7501 agctcatatt ttaagggaca atgctaaaca ttaaattact aagagagaga cctcaatttg
     7561 tggcagagag gcttgcagtc aaaaactttg acgcaactga aataataaaa agtatcaatg
     7621 agttggatca gaaaagaaga gaaactcaaa cggaattgga tactattctt tcggaacaga
     7681 atcttctttc aaaaaagata gggttgttaa tgaaggatgg taaaatagtt gaagcagagg
     7741 aggttaaggc caaagtttct gatttgaagg aaaagtcaaa gttgcttgaa aacgagatgc
     7801 agatagtttc ttctaaaatg aacgaacaat tgttactgct tccaaatttg cctcatatat
     7861 ctgttccttc aggtaaaagt gcagaggata atgtgatagt aagagaagga ggatctattc
     7921 cggaatttga taagagtgct ttgccacact gggagcttgc tagaaaatat gatattattg
     7981 attttgatct tggggttaag cttactggag caggttttcc tgtttataaa ggtaaaggtg
     8041 caaaaatcca gagagcgctt atttcttatt ttcttgataa aaatacaaat gcaggttata
     8101 cagaatttca acctccgctt atggtgaatg aggcatcagg atatggaacg gggcaattgc
     8161 ccgataaagg agagcagatg tactatgtga atctcgataa tttctatcta attcctactg
     8221 ctgaggtgcc tgtgactaat atattccgtg atgttatatt gggttattca gaccttccag
     8281 tgaagtgtac tgcctataca ccttgtttta gaagagaagc tggttcatat ggcaaggatg
     8341 ttcgcggtct gaaccgtctt catcagtttg acaaagttga gatagtacag ataactcatc
     8401 cggataattc gtacgatgct cttgaagaga tggtcaagca tgttgaaggc attgttcgtt
     8461 ctctttgtct cccttacaga attttgcgac tttgcggagg ggacatgagt tttacatctg
     8521 ctcttacata cgattttgaa gtctattctg ctgcacagag aagatggctt gaagtaagtt
     8581 cagtgtcaaa ctttgaaaca ttccagtcta acaggctgaa actccgtttt aaagatgaaa
     8641 atggcaaaac tcaacttgct cacactttga atggaagttc tcttgctctt ccaagaatac
     8701 ttgcggcttt gctggaaaac aatcagacag aaaggggaat agaaatacct tctgttttga
     8761 gagaatatac aggattttcc tatatcgatt aatttagatg gatgacgaga agattatact
     8821 tggcattgat cccggtacaa gaattcttgg ttggggtgta gtgagaagct ttggcaacag
     8881 tgtcgatttt ttggatatgg gtgtggtaga tatgcatagt gaaacagacc attatattaa
     8941 actcggcata attttcaggg aagtttctgc tctcataaat cgtttcaatc ctacagacct
     9001 gtctattgag gctccatttt atggcaaaaa tgttaagtct atgtttttgt tgggaagagc
     9061 acaaggggca gctattactg ctgctcttgc cttgggcatt cctgtttctg aatatgctcc
     9121 tcgaaaaatt aaaatgtcaa taacaggtag aggggctgct tcaaaagaac aggttgccaa
     9181 tcttctatac aacatactca aaatagaaca atctgtaaat tattttgatg ctactgatgc
     9241 cctggctgtt gcttactgtc attttcttga aacgagtaat ccgtattttg gtaaaggttt
     9301 taatgaaagt tggaagagtt tcgttaagcg taattccagt cgagtgaaat aattttttgt
     9361 tttgatttaa cctttttcat ttttgtgtgt caaagcagaa agtttcttaa aatagactaa
     9421 agaataaata attatgagta tgaagtatat taataaaata tacctggtgg ttgtttgtat
     9481 gattttttta aactcaacaa aaacttttgc tgttgatgta gagcctaaag atttcaggat
     9541 aaagataata ctcattgata gcgtaactca ggatccaatt gaatttgcta ctgtttccat
     9601 aacacctgag ggcaagaagg aggccttgaa atatgctctt actgatgcat caggctgggc
     9661 tgaaattgtt aaagttccag agggtacata tacactgaaa gcagagtgtt taggttattt
     9721 accttttgaa actaaagtaa atacaacagg taaaagtctt ttagatcttg gcagggttaa
     9781 gatgaaacag gagataaacg ttttgtcaac tgtgacagtt acagctcttg gaaaccctat
     9841 tattgttaag aaagacacaa ttgaatataa tgcctcgtct ttcaaagtta cagatcaaga
     9901 tatgcttgag gctctcctca agaaattacc tggtattgaa gtctcaagcg atggtacaat
     9961 tacagcaaat ggcaagacaa tagacaagat aacaattgat ggtaaaacat tttttttcaa
    10021 tgatccacag ttagctacaa aaaatcttcc tgctaaaatt atcgataagg tcaaggtgct
    10081 ggaaaagaaa tctgaacagg ctcagtttac tggtattgac gacggtcagt ctgagacagt
    10141 tatagatcta tcaatcaggc cggggatgat gaacggatgg tttggaaatg ttaccggtgg
    10201 ttatggtact gacgataagt atcaggttgc aggaatgttg ggtaacttca cttccaaaag
    10261 ccagataagt atgattgcga atgccaacaa caccaataat agaggttttt ttgatgtggc
    10321 cggtgatgct atgagaagta tgagaaattc acttggaggt gccggggggg tgcgaatagg
    10381 aggagcagtt atgaattttg gaggatcagg aatcacaact tcatggatgg gtggagttaa
    10441 tgccaatacg gtaagtaata acaaaaagtt gaatattgga ggaagttacc tctacagtgg
    10501 aagcgatact gaaaacaaac aggttacatc gaagcagaca tttcttgcag acagtagttt
    10561 tatctataat agtaatcaat attcaaagag cagtactgat gccaacagcg cttctgtcaa
    10621 tcttgaatat aagttcaatg ataaaacatc tattttgttc agacccaata tgaatatcgg
    10681 aagtggatcc tttaatgaag aaaatgtata ttttacaaag ggtgtgctcg gaagttcaat
    10741 aaatgacggt agcagcaaat cttatggcaa tagtgatact agatctttaa acggggatct
    10801 tttattaagg cagaagttta ataagacagg caggacgttt tctgttaact ttaattatgg
    10861 atacagtaat aatgatatag aagccttcaa tgagtcaact accaatagct atggcactaa
    10921 tccactcact acagaagtaa gacagaagta taatacagaa caggacaatt attctctcgg
    10981 cgggagagct tcatatacag agccacttgg taatcattat tacatggagt tggcttattc
    11041 ttacaacttc agaaaaaata attctgataa agttgcaaaa aactttaatc aaaataccgg
    11101 taattatgat ctttatgatt caacctattc aaacagtttt gagaatacat ttatcaatca
    11161 aataggagag cttaatatca gaaaagttga ggaaaagtac agctttactc ttggaatgaa
    11221 tgtccaacca gcttatacac atagtataac tacatctgat cttaaaacta ctgatcttga
    11281 aagaagtgtt gttaattatt ctccttcagc tttgttagat attgacttta gtgattccaa
    11341 atctttgaga ataagatatc gtggcagcac aagacagcct tcaataagcc agatgcagcc
    11401 agtgaaagat aattccaatc cgctttttgt gtctgaagga aatccagatc ttcttcctga
    11461 attttcacac aacctttctc taaacttcag aaataccaac aggagcaatt tcagtacagt
    11521 caatgccaga ttggatggaa cttatactct cgacaagatt gcgaacaaaa cctggtatga
    11581 agagggtgga atccagcaca caaaacctgt aaacgatcaa gctgtctact caatttctgg
    11641 aaatattatg tggaatacac cgatagagaa aacaaagaag ttttatttta tgacaaatac
    11701 cagagcaggt attaataatg ggataaacta ttccagcagc actgcttctg aaacaggtgc
    11761 agtgaagaat ataacaactt ctctctctgt ttcagaaatg cttagattgg catatcgtgg
    11821 cgagaagctt gagattactg caggaggaag ggtttcttat gccaaggctt ggtacactat
    11881 aggtgaaaca aaagatgcca catggaataa ttctctgaat gctactatta actggacttt
    11941 gcctgcaggc tttggtcttg caagtgatat agagcaccag ttttatgttg gatatggaga
    12001 aggatataat gatccggcga ctgtttggaa tgccgaggtt tccaagttgc ttttcaaaaa
    12061 ctctgcttct ttgatattta gagtatttga tatattggac cagtccaaga gtgttagaag
    12121 gactactaca gacaactata ttcaggatgt cagcagtctg actctcaaac aatattttat
    12181 gctctcattt acgtggcgtt ttggaaaatt tggggacaga agcggtcagc aaggaggctc
    12241 cagaggtatg aatcatggaa gattcggccc tccaggtgga gtatttcaca ggtgattatg
    12301 ctaacgtttg taatgagaat aacttctcca tttttcaata acagagagca tatctgaagg
    12361 tacgggagac tcaaaaaaga gtctctcgcc tgtttttgga tgagtaaagc cgatagttct
    12421 tgcatgtaag gcctgtcgag ggagcaattc aaagcagttg tctacaaact gtttgtattt
    12481 ggcatagaaa gttccattga ctattttatc acctccatat ctatcatcgt tgaaaagagg
    12541 atgcccgatg taatccatat ggactcttat ttggtgtgtt cttcctgttt ccagtctaca
    12601 tgaaacaagg gttgtatagc caaatctttc aataacttta taatgtgtta ttgcatgttt
    12661 cccaatggaa ccatcagtaa aaaccttaan nnnnnnnnnc cttaaaacgc attcagtcgt
    12721 ttggatctct cgaaatattg cctgtaatag tgccactatc ttctttgaga ttgccccaaa
    12781 caagggcaac atattcccgg tcgatagtgt gatcaaagaa ttgacgtgca aggttaaact
    12841 gtgcttcgtc atttttagct actaacaaaa ggcctgatgt gtttttatcg attctgtgga
    12901 ctaaaatacc catcctttca tcaggagcat tttcatcttg acttataccg agatggaagg
    12961 cgagagcgtt tacaagagtc cctgaaaaat ggccgtggcc tggatgaact acaagcccgg
    13021 gcggtttatt aatcagtaac aggtcgtcat cttcgtagat aatatcgaga ggaatgtttt
    13081 caggcttaat ctccatgccc cttctttggt atggaagttc aatagttatt atatcaagtg
    13141 gtttaactat ataacttgcc tttgccctct tgccgttaac tagaacatag ccggcatcaa
    13201 ttgcgagctg tattctgtga cgggaggttt tctccatatg gctggtaatg aacttgtcaa
    13261 ctcttacagg tgtttgaccc ttatccgaga tgaaacggta atgttcaaag ctgactacat
    13321 cttcttcatc ttcaagcatt ctactcggtt tttatagctt ttgatttttc cttattgatg
    13381 ctaagaaaaa gttcaacttc tgtgcccaaa ggataggagg ttactctggt aggagaggga
    13441 atctgtctga ttacaacgct tgaaacagta tcagagtaac ttttgacgag gttgtcatag
    13501 tgtatttttc cgaggttcaa agaattttcc aagagtattt ctttggcaag ctgaagggaa
    13561 actcctgtta catctggaat agatgtagct gattgtccgt tatcgccaag ttctagatcg
    13621 atttggcttc cagcttcaat ttttgttcct ggggcaatga aacgtccatt aaatttctgt
    13681 gataatacat tgtctgttgc catgtcatag acataagtaa gtctgccgac actaagctgt
    13741 ttggcttcca gttcagccaa cgcttgacgt aaggaaaatc ctgttactga aggcatagtg
    13801 acaagttttg gttggattgt att
//
LOCUS       DAOTQL010000034        13260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4383_length_13260_cov_5.476789, whole genome shotgun sequence.
ACCESSION   DAOTQL010000034 DAOTQL010000000
VERSION     DAOTQL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_13260_cov_5.476789"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..630
                     /locus_tag="P5293_03405"
     CDS             187..630
                     /locus_tag="P5293_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48979.1"
                     /translation="MVNFFGEFTVKMDDKNRLVLPSSFKSFLKDGDMRFVVRKEIFSD
                     CLEMFTFEDWNAESEKIRAKLNMNNREHALLWREYNREVALVTPDEKMGRISIPKKLL
                     EKIGIVKEVVFVGIGNKIEVWASEKYQNSKLSDNDYLSLLEKILG"
     gene            645..1547
                     /gene="rsmH"
                     /locus_tag="P5293_03410"
     CDS             645..1547
                     /gene="rsmH"
                     /locus_tag="P5293_03410"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRR48980.1"
                     /translation="MSDYHTPVLLDESVSALAISPGGVYVDATFGGGGHSREILSRMD
                     KKSRLIAFDRDEEALKNAPDDYRLTLINNNFRFIRNFIRLEGYEYVDGIICDLGVSSH
                     QFDTGERGFSFRYDAPLDMRMSSNSKLTAADVVNAYSEEEMETLFADYGEVENSKKVA
                     NLICKYRAENTIMTTTDLNKAIEPLIPRFNEYKYLAKIYQALRIEVNGEMRALEGFLH
                     GALFSLKKGGRVVIITYHSLEDRMVKNFLKSGNISGLSERDFFGNILSPFKVITKKPI
                     IPSETEVASNARARSAKLRVGEKM"
     gene            1555..1878
                     /locus_tag="P5293_03415"
     CDS             1555..1878
                     /locus_tag="P5293_03415"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRR48981.1"
                     /translation="MKVKQIIDDIAGGMWLVKTGIFKHAYFVIYIFFLILVYIGMSYG
                     FENSQIKERRNLGIIKDLKADYTGKTAKLLYQSKRLEIEKRLVEYNSKLKNPTEPPKR
                     VIIER"
     gene            1880..3925
                     /locus_tag="P5293_03420"
     CDS             1880..3925
                     /locus_tag="P5293_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRR48982.1"
                     /translation="MKTEMNRAFVVYALFVLIGIAIIARIVYLQFVASEDLSNEVKTF
                     RVEEIEANRGSILACDGRILSASVPYYKIKMDCVAPEQAVFDKFKDSLAIALSNFFGD
                     NTAAWYKKRIENARAIKDRDISLGNRLVDHSELVTIKNFPLFKLGSLKGGFIYEQKNK
                     RNNPYGRLAYRTIGFINSNGQGVGIEESFDYYLKGTPGRQVVQRVLRNQWKPVDIDKI
                     IPPQDGYDVQTTLDIDIQEAAEAALKEQLSLSDQFEGATAIVMEIQTGAVRAIANMRK
                     DSNGNFDEVFNYAIGQATEPGSTFKLVTLVSLIEDGYVTLETPVDAGNGKWTYAGHTI
                     SDVTAGGYGQINVLKAFEKSSNVAFAKLTVEFYANNEKHFVERINNMKVNEKFNLDIK
                     GEARAVIYSPGDVSWSKLTLPLMGIGYATLLTPMHTLAFYNAIANGGKMMKPYFVDNI
                     QRNGVIEKKFEPVELSGAICSKSTIETVHKALRSVVEEGTAKALNDKRYSISGKTGTA
                     RIAFNGSGYTDREGYRRHQASFAGFFPSNNPKYSAIVVLYTGRTKGNFYGGGWAAPVF
                     KKISDKIYVASPEWNEPVKGNGNISLKKTKIITQLEESMKCDSIPQVTGMGIRDAIYI
                     LENQGYNVKFSGKGRVVAQNFFPGNNSDNKGYIYLQLSDKYETSEDTLKMQSVADNR"
     gene            3873..5321
                     /locus_tag="P5293_03425"
     CDS             3873..5321
                     /locus_tag="P5293_03425"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRR48983.1"
                     /translation="MKLLKILSKCKVLQIIGNEEIDIKTVCFDSRKVGKGALFVAFKG
                     VTSDGHDFIRQAVEAGATAIVCEKIPEEQLPATIIQVDDSHLALSIIASNFYGNPSES
                     LKLIGITGTNGKTTTVTLLYRLFTELGYKCGLLSTIANFVVDKQVGATHTTPDPIEIN
                     ELLSQMAECGCEFCFMEVSSHSLDQKRVAGLHFTGAIFSNITHDHLDYHKTLEEYIRV
                     KKSLFDNLPSTAFALVNIDDKNGMVMLQNTSAKKYTYSCHSPANFNCRIIEKSMDGML
                     LNIDNREVWTRFIGEHNAYNILAVYATAVILGANKEEVLLKLSNMTSVAGRLEYLKGK
                     NDITAVVDYAHTPDALENVLKTLRETARGKKLTTVFGCGGNRDRSKRPEMAEIAAKYS
                     DRIIVTSDNPRFEDPDEIIKEIKNGFNHNDLSKTIFITDRKEAIRTAIITALPGEVIL
                     VAGKGHEDYQDVKGEKHHFDDKEIIAETFNQL"
     gene            5336..6616
                     /gene="mraY"
                     /locus_tag="P5293_03430"
     CDS             5336..6616
                     /gene="mraY"
                     /locus_tag="P5293_03430"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRR48984.1"
                     /translation="MLYNLFEYLNGKIDFPGMGLFKYISFRAISASILSLLIALFVGK
                     KIIKTLTKKQIGETIRELGLEGEQQKKNSPTMGGIIILISFLVPTLLLADLTNVYVIL
                     MIITALWLGFVGFIDDYIKVFKKNKQGLSGKFKIIGQVSLGLVVGLTLYFSDQAVIRV
                     PSSQRGSEARPEIVSQQGFSDKITFFKEEKSTKTTIPFFKGNEFDYSWLSPVGGKWEE
                     LAGWIIYIAVIIFVVTAVSNSTNLTDGLDGLATGISAIEVATLGVLAYVSGNIIYSNY
                     LNIMYIPYTGELVIVLAAFIGALIGFLWYNSYPAQVFMGDTGSLAIGGIIAVSAIVIK
                     KELLIPIFCGILLAESLSVIIQTSYFKYTRIKYGAGRRVFRMTPLHHHFQKIDGDALI
                     RIPKRIIPEAKIVTRFWIIAILLAVFTLITLKIR"
     gene            6618..7952
                     /gene="murD"
                     /locus_tag="P5293_03435"
     CDS             6618..7952
                     /gene="murD"
                     /locus_tag="P5293_03435"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRR48985.1"
                     /translation="MSRVVILGGGESGVGAAVLAKIKGHETFLSDNGMLSQEAKESLE
                     RWKIDYEEGRHTRELILKADEVIKSPGIPDSAPIIEDIKVKGIPVISEIEFAGRYDRA
                     KKICITGSNGKTTTTSIIYFLMKNAGLNVGLAGNIGQSYAYQVATQDYDYYVLELSSF
                     QLDGMYDFKADIAVLLNITPDHLDRYDNQMEKYIKSKFRITQNMSESDCFIFCADDEI
                     SISHLNEIVLKAKQLPFSQKEKFDEGAFIEEEQIKVRYKDDEYNMFLNELSLQGKHNI
                     YNSMAAAITGKVLNIRNEVLRQSLSSFKGVEHRLEKVLKVKNVLYINDSKATNVNSTW
                     YALESMTTPVIWIAGGKDKGNDYTPLFDLVKEKVRAIICLGADNTKLHNVFGNLVEEM
                     YDTNSAEEAVKIAYNIAQPGETVLLSPACASFDLFKNYEERGRLFKEAVKKL"
     gene            7954..9168
                     /locus_tag="P5293_03440"
     CDS             7954..9168
                     /locus_tag="P5293_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRR48986.1"
                     /translation="MSEPNPIIYRLRGDKVIWTVVLLLSLTSLAAVYSSSCSLALREG
                     KTTFDILLRQLSSIVLGLTALYICYKIPLKYYRSFANILMLVSIGLLIATIFVGKSIN
                     SASRWIEIFGLSFQPSEIAKICTILYIAKIVEKNNLETFKKFTLLILVPVSIVLVLIL
                     WGSASQALLLAFICCLILYISDVKRSHLLKSAGIIGAGLILILLLHFSFGIFGRIDTA
                     ISRIKNNDTEQLNNKNLSPEDRKELENSTFQKRMAKVAVSSAGIIGKGPGKSTQRYVL
                     PHPYSDFIYAIIVEEYGLIGGVIVLMLYLWLLFRCMTLAYSCTKPFSRIVVIGLGLMI
                     TFQALLHMMVNVTLIPVTGHTLPLVSLGGTSIIIMGGAFGIILSISRTIEITKENNTK
                     KIEVNVGEENYN"
     gene            9143..10249
                     /gene="murG"
                     /locus_tag="P5293_03445"
     CDS             9143..10249
                     /gene="murG"
                     /locus_tag="P5293_03445"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRR48987.1"
                     /translation="MSEKRIIISGGGTGGHIFPAISIANSLKKIDPECEILFVGALGR
                     MEMEKVPAAGYKIVGLPVAGLKRELSVENLKLPFKIFKSLCQARKIVYDFDPDVAVGV
                     GGYASGPVLWMASRMNIPILIQEQNSYAGLTNRILGKKAKKICVAYEGMEKFFKAEKI
                     ILTGNPVREGIVLINPQQKEEAYRYFELDPQKKTILIIGGSLGASTLNNCAKEWIRKG
                     TQENIQLIWQYGSFYKNEIRDFISANPKPNVKAFEFIKRMDYAYAIADVVISRAGAGT
                     ISELAVAGKASVLIPSPNVVKDHQTHNAMALVRKNAAIMIPDDEAVEKAFKAAVDLAE
                     DENKRKELETRISGMAIRNSAEIIAREVLKLAEK"
     gene            10246..11619
                     /gene="murC"
                     /locus_tag="P5293_03450"
     CDS             10246..11619
                     /gene="murC"
                     /locus_tag="P5293_03450"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRR48988.1"
                     /translation="MNFVYLIGIGGIGMSALARYYNHAGAIVAGYDRTPSKLTSELEK
                     EGIRIHYEDDIALIPPEFTSYKEKTLVIYTPAIPDNHSEYQYFLKNGYNIVKRSRALG
                     NIASQKRCLAISGTHGKTTTSTLLAHILTQSGKGCTAFLGGISKNYHSNLLLDKRDYL
                     VAEADEFDRSFLQLYPEMAVITSTDADHLDIYSTHNKIKEAFLEFASQVKPDGALIVK
                     KGTDIDLSSVKAERVYEYSYDIPCDFYASSIKVLEDGFFNFDLNYPGGVIKNCTVGIP
                     GWINIENSIAASSIALLEGISPEDLKNSLKTFAGVERRLDIRVKNEKCSYVDDYAHHP
                     KELSSAISSLREIFAGRKITGIFQPHLYTRTRDFAEEFAKSLSLLDELILLDIYPARE
                     EPIEGVTSEIIYKNVSLDSKIMLHKEELINYLAKIDIDVLVTFGAGDIDRFVNPITQL
                     VSAKSKC"
     gene            11613..12413
                     /locus_tag="P5293_03455"
     CDS             11613..12413
                     /locus_tag="P5293_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR48989.1"
                     /translation="MLKKTLIIILYLLLTGVLCCYFIFSSKITKEGKMHEMCHQIKVV
                     ILDSAEIRFVSKTEIADLIVDNGITPGESKLRHINNYQIEQLLNKRTAVKKSEVSYTR
                     DGILRVIILQRKPILRLQTENGGFYMDENAYVFPLIKGFSPFVPVVSGEIPLEIPPKY
                     NGYVKNNEQWANSLKDLCLYILKNESWDALIEQIYVDSRGILSFIPRDGDFEIIFGNM
                     DKIEYKFKKLYAFYKDILPNVGPEKYCKINLQFDKQIVCKKTNKNIEI"
     gene            12410..>13260
                     /gene="ftsA"
                     /locus_tag="P5293_03460"
     CDS             12410..>13260
                     /gene="ftsA"
                     /locus_tag="P5293_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRR48990.1"
                     /translation="MKKLVAAVDLGTTKVVSVVGEKSESGYRIVALKEAPSKGVMRGE
                     VVNIQNVLESLKPTLEEIRAEGIEISEVYVGIAGQNIKCNSARISRNRSRSSEWITNR
                     EVEEMEREMFNSRVNAGEKILHVIPQSYNVDDNVGVKEAVGMTGTRIEGDYKLFIGKT
                     NSFEHSKIVIERAGLKLKDIILEPVASAKAVLTEDEMELGVAMVDMGGGTTDLLIIQD
                     NIIRHTAVIPFGGNSITEDLRQGCGVSLRNAEQMKIQYGSCYSDRAPDNKTVIIPGLG
                     GRDSREVA"
BASE COUNT         4586 a         2320 c         2725 g         3629 t
ORIGIN      
        1 cccaatatat tttcatcccc tttggcgttt attttttagt catggatgtt tcatgttatg
       61 ttatttcagg ccgaagttaa taatttcagg gaaattgttt gtaatttggt gtaaaagttt
      121 gtaaaaattt gggtaatatt gtaaaaagat tttatatttt tgcataacaa agaaattgga
      181 aattcgatgg ttaacttttt cggggaattc actgttaaga tggacgataa gaacagatta
      241 gttctaccat catcgttcaa atcctttcta aaagatggag acatgcgctt tgtagtaaga
      301 aaggagatct tttcggactg cctcgaaatg ttcacgtttg aggactggaa cgcagaatcg
      361 gagaaaatac gtgccaaatt aaacatgaat aacagggaac atgctctttt gtggcgtgaa
      421 tacaatcgcg aagtagcact tgtcacccct gacgaaaaga tgggcagaat ctccattccc
      481 aagaaattac ttgaaaaaat aggaattgtc aaggaggtgg tttttgtcgg gataggcaac
      541 aaaatcgaag tttgggccag cgaaaaatac cagaactcca aattatccga caacgactat
      601 ctttcattac tggaaaagat attaggataa ggaggcacta catcatgagt gattatcata
      661 cacctgtcct gcttgacgaa agtgtgtcag cccttgcaat atcgcctggg ggtgtatatg
      721 tagatgccac attcggagga ggaggacaca gcagagagat cctttcgagg atggataaga
      781 aatccaggct tattgctttt gatcgagatg aggaagctct taaaaatgct ccagacgact
      841 atagattaac attaataaac aacaatttta gatttataag aaatttcata agattagaag
      901 gttatgagta tgttgacgga ataatttgtg atttgggtgt atcatcacat cagtttgaca
      961 caggagaaag aggtttttcg ttcagatatg atgcacctct cgatatgaga atgagcagta
     1021 attcaaagtt aacagcggca gatgtggtaa atgcatattc agaagaagaa atggaaactc
     1081 tttttgccga ttatggcgaa gttgaaaaca gcaagaaggt tgctaatctt atctgcaaat
     1141 acagagctga aaatacaata atgactacaa ccgatctaaa caaagcaata gaaccattaa
     1201 taccaagatt taacgaatat aaatacttgg ctaaaatata tcaggcacta agaattgagg
     1261 ttaacggtga aatgagagca cttgagggat ttcttcacgg agcactgttc tctctcaaaa
     1321 aaggaggaag agtagtgata attacctatc attctcttga agacagaatg gtcaaaaatt
     1381 ttcttaaaag cgggaatatt agcgggttat ctgaaagaga cttttttggc aatattcttt
     1441 caccttttaa agtaataaca aagaaaccaa tcataccatc cgaaactgaa gttgcatcaa
     1501 atgcaagagc aagaagtgcg aaacttagag ttggagagaa aatgtaaata actgatgaaa
     1561 gtcaaacaga tcatagatga tattgccgga ggaatgtggc tggtcaaaac aggcatcttt
     1621 aaacatgcct attttgtgat ctatatattc tttctcattt tggtgtatat tggaatgagc
     1681 tatggctttg aaaacagtca gataaaagag agacgcaatt tgggcataat taaagacctt
     1741 aaggccgact atacaggtaa aactgccaaa ctgctgtatc aaagcaagcg actcgagatt
     1801 gaaaaaagac ttgttgaata taattcaaaa ctaaaaaatc ctacagagcc tcctaaacgt
     1861 gtaatcattg agagatagta tgaaaacaga gatgaataga gcttttgtag tatacgcact
     1921 ttttgtgtta atcggcattg cgattattgc aagaattgtg tatttgcagt ttgtagcctc
     1981 tgaagatctc agtaacgaag ttaaaacatt tagagtagaa gaaattgaag ccaaccgtgg
     2041 aagcattctg gcatgtgatg gtagaatact ctctgcctct gttccatact acaaaattaa
     2101 aatggattgt gttgcaccag aacaggctgt gtttgataaa tttaaagatt cattggcaat
     2161 tgcacttagc aatttttttg gagacaatac tgcagcttgg tacaaaaaaa gaattgaaaa
     2221 tgcaagagca ataaaagaca gggacatatc gcttggcaac agactggtcg atcactctga
     2281 acttgtaaca attaaaaatt tccctctttt caaactcggt tcacttaagg gaggcttcat
     2341 ctatgaacaa aaaaacaaaa gaaataaccc gtatggaaga ctggcatacc gcacaatagg
     2401 atttattaac tctaatggac agggtgtggg aatcgaggag agttttgatt attacctcaa
     2461 aggaacacca ggtagacaag tagttcagag agttctcaga aaccagtgga agcccgttga
     2521 catcgataaa attataccac ctcaggacgg atatgatgtt cagactacac tcgacataga
     2581 catccaagaa gcagcagaag cagctcttaa agaacagctt tctctttctg accagttcga
     2641 aggggcaact gctattgtta tggagataca gaccggtgct gtcagagcaa ttgcaaacat
     2701 gagaaaggac agcaatggaa attttgatga ggttttcaac tatgcaattg gccaggctac
     2761 agaaccggga tctactttta aacttgtcac ccttgtctct cttatagaag atgggtatgt
     2821 gactcttgaa actcctgtcg atgcaggaaa tggcaaatgg acttatgccg gacatactat
     2881 ttcggatgtt actgccggag gatatggcca aatcaacgtt ttaaaagcat ttgaaaaatc
     2941 gtcaaatgtg gcatttgcca aacttacagt tgaattttat gccaacaacg agaaacactt
     3001 tgttgaaaga atcaacaaca tgaaggtcaa tgaaaaattt aatttggata tcaaaggaga
     3061 ggcaagagct gttatatatt caccaggaga cgtatcttgg tcaaaactta cacttcctct
     3121 catgggaata ggttatgcaa ctctgctgac tcccatgcac actttagcct tctacaatgc
     3181 tatagctaac ggaggcaaaa tgatgaaacc atattttgta gataatattc aaagaaacgg
     3241 agtaatagaa aaaaaatttg agcctgttga attgagtggt gcaatttgtt caaaatctac
     3301 catagaaact gttcacaaag cacttaggag tgttgttgaa gagggaacag ccaaagcttt
     3361 aaacgacaag cgttatagta tctcaggcaa aacagggaca gcgagaatag cttttaacgg
     3421 ttcgggatat actgacaggg aaggatatag aagacatcag gcatctttcg caggattttt
     3481 cccgtcaaac aacccaaaat actctgctat agttgtttta tacacaggaa gaacaaaagg
     3541 taatttttac ggtggtggct gggcggctcc agtctttaag aaaatttcag ataagatata
     3601 tgttgcttca ccagaatgga acgaaccagt gaaaggcaac ggcaacattt cattgaaaaa
     3661 gactaagata attactcaac tcgaagagtc aatgaaatgt gactccatac ctcaagtaac
     3721 cggaatggga atacgtgatg caatctatat cctggaaaac caaggatata atgtaaaatt
     3781 tagcggaaag ggaagagtgg ttgcacaaaa tttcttccct gggaataata gcgacaacaa
     3841 aggatatatc tatttacaat tatcagataa atatgaaact tctgaagata ctctcaaaat
     3901 gcaaagtgtt gcagataata ggtaacgagg aaatcgacat aaaaactgta tgttttgatt
     3961 cacgtaaagt tggcaaaggt gctctctttg tcgcattcaa aggtgtcact tcagacggtc
     4021 acgactttat ccggcaggca gtcgaagcag gagccactgc tattgtttgt gaaaagatac
     4081 ctgaagaaca attacctgca acaataatac aagtagatga cagccatctt gctctaagca
     4141 ttatagcatc aaacttttat ggtaatccct ccgaaagttt aaaacttatt ggaataacag
     4201 gcaccaatgg aaaaacgacc acagttacat tgctttacag attatttaca gaactcggct
     4261 acaaatgtgg tttgctctct acaattgcta atttcgtagt agacaagcaa gtcggtgcaa
     4321 cacatacaac tcccgatcca attgaaataa acgagcttct ttcacaaatg gctgaatgtg
     4381 gatgtgaatt ctgttttatg gaggtaagtt cacattctct cgaccagaaa agagtcgcag
     4441 gacttcattt tacaggagct atcttctcaa atattacaca cgaccatctt gattatcaca
     4501 agaccttaga agagtatatc agagtcaaga aaagtttgtt cgacaatctg ccttctactg
     4561 cttttgcgct tgtcaatatt gacgacaaga acggaatggt tatgctgcag aacacatctg
     4621 caaaaaaata tacctactca tgccactctc ctgcaaattt taattgcaga ataattgaaa
     4681 aaagcatgga cgggatgttg ttaaatattg acaatcgaga agtatggaca agatttatag
     4741 gtgaacacaa tgcatataat attctggcag tatatgctac tgcagtgata ttgggagcta
     4801 acaaggaaga agtgctgctt aagctaagca atatgacatc agtagcaggc aggttagaat
     4861 atcttaaagg caaaaacgac attactgctg tagttgacta tgcacataca cctgacgcac
     4921 ttgaaaatgt actgaagact cttcgcgaga cagccagagg taagaaactc acaaccgttt
     4981 tcggatgtgg aggtaaccgt gacagaagca aaagacctga aatggccgaa attgcagcca
     5041 aatattcaga tagaatcatt gtaacttccg acaatccacg ttttgaagac ccggatgaaa
     5101 taattaaaga gattaaaaat ggctttaacc acaatgattt gtcaaagaca atttttataa
     5161 cagaccgtaa agaagcaatt agaaccgcaa taattactgc cttacctgga gaagttattc
     5221 tcgttgcagg caaaggtcac gaagactacc aggatgtaaa gggtgaaaag catcactttg
     5281 acgataagga gataatagca gaaactttta accaattata aaataataac aggagatgct
     5341 ttataatcta tttgaatatt taaacggaaa aattgatttc cccggaatgg gactttttaa
     5401 gtacatctcc ttcagggcta tttctgcctc aatattatct cttctgatag cactgttcgt
     5461 tgggaaaaaa attattaaaa cccttacaaa aaaacaaata ggagagacca tcagggaact
     5521 tggcctggaa ggtgaacaac agaaaaaaaa ttctcctacc atgggtggga taattattct
     5581 gatttcgttt cttgttccaa ctcttcttct tgccgatctt acaaatgttt atgtgatcct
     5641 tatgattatc acagccctat ggttgggatt tgtcggtttt atcgatgatt atattaaggt
     5701 atttaagaaa aacaagcagg gtctatccgg taaatttaaa attataggtc aagtctctct
     5761 tggtttagtt gtaggactga cactgtattt tagcgatcag gcagtgataa gagtaccttc
     5821 ttctcaaaga ggtagtgaag cacgccctga aatagtttca caacaaggat tctccgataa
     5881 aatcacattt tttaaggaag aaaaaagtac aaaaactacc ataccattct tcaaaggaaa
     5941 tgaatttgac tactcctggc tctctccggt tggagggaaa tgggaagaac ttgcaggatg
     6001 gataatatat atagcagtta tcatatttgt tgttacagct gtgtcaaaca gtacaaacct
     6061 aaccgacgga cttgatggcc ttgccacagg tatatcggca atagaggtgg cgacacttgg
     6121 agtgctcgca tatgtatctg gtaatattat ctattcaaac tatctgaata ttatgtatat
     6181 cccatatacg ggagaacttg taatagtact tgccgcattt ataggagctc taataggttt
     6241 tttatggtac aactcctacc ctgcccaggt ctttatgggt gatacaggaa gccttgcaat
     6301 cggaggaata attgctgttt ctgccattgt tataaaaaag gaactgctta ttcccatctt
     6361 ctgtggcatt ctgctggcag aaagtctttc cgtgattatc caaacatctt attttaagta
     6421 tacaagaata aaatatggag caggaagaag agtattcaga atgactcctc ttcaccatca
     6481 ttttcagaaa attgacggag atgcgttaat acgtatccca aaaagaataa ttcctgaagc
     6541 aaagattgta acaagatttt ggataatagc cattttactt gctgtattta cattaataac
     6601 attaaaaata agataatatg tcaagagtag tcatattggg aggaggcgaa agcggcgttg
     6661 gagctgctgt ccttgctaag ataaaaggac atgaaacatt tctttcagac aacggcatgc
     6721 tttcacagga ggcaaaagaa tcacttgaaa gatggaaaat agattatgag gaaggaagac
     6781 atactcgcga acttattctg aaagcagacg aagttataaa aagtccaggc atccctgatt
     6841 cggcgcctat tatcgaagac ataaaagtta aaggtatacc tgtaatttca gaaatagagt
     6901 ttgcaggaag atacgataga gcaaaaaaaa tctgtatcac aggcagcaac ggtaaaacta
     6961 ccactacctc aataatatat tttttaatga aaaatgcagg tctgaacgta ggccttgcag
     7021 gtaatatcgg acaatcctat gcatatcaag tcgcaacaca ggattatgac tattatgtac
     7081 tcgaacttag cagcttccag ctggatggaa tgtatgactt caaggctgat atagctgtat
     7141 tgctcaatat cactccagat catcttgaca gatatgacaa tcaaatggag aaatatatta
     7201 aatcaaaatt cagaataact caaaatatgt ccgaaagcga ctgctttatt ttttgtgcgg
     7261 atgatgagat aagcatatcc catctaaacg agattgttct caaggcaaaa caactgccgt
     7321 tcagtcaaaa agagaaattt gatgagggtg catttattga agaagaacag attaaagtca
     7381 gatataaaga tgatgagtat aatatgttcc tcaacgagct ttcacttcag gggaaacata
     7441 atatttacaa ctccatggca gcggcaataa caggcaaagt acttaatatc agaaacgaag
     7501 tacttcgcca aagcctttcc tcttttaaag gagttgagca cagacttgag aaagtgctga
     7561 aggttaaaaa tgtgctatac ataaatgact ctaaagccac caatgtcaat tcaacatggt
     7621 atgcacttga aagtatgaca acccctgtaa tatggattgc tggaggcaag gataagggga
     7681 atgattatac tcctctgttt gacctggtaa aagaaaaggt tagggcaatt atatgtcttg
     7741 gcgctgacaa tacaaagcta cacaatgtat ttggcaatct agttgaagaa atgtatgata
     7801 caaattctgc cgaagaagct gtgaagattg catataacat agcacaacca ggtgaaacag
     7861 tattgctttc gcctgcatgt gcaagctttg atttattcaa aaattacgag gaaagaggaa
     7921 gattatttaa ggaagcagtt aaaaaacttt aaaatgagtg agcccaatcc aataatttac
     7981 cgtctgagag gcgacaaagt aatatggaca gtagtactgt tactttctct tacatcactt
     8041 gcagctgtat actcatcaag ctgctctctg gcgttaagag agggtaaaac cacctttgac
     8101 atacttcttc gtcaactttc atcaattgtt ttgggattga cagctctgta catttgttat
     8161 aaaatacctc ttaaatatta cagaagcttt gcaaatattc ttatgctagt aagcattgga
     8221 ttattaattg caacaatttt tgtcggaaaa agcatcaaca gcgccagcag gtggattgaa
     8281 atattcggct tatcttttca gccctccgaa atagctaaaa tatgtacaat actctacatt
     8341 gccaaaatag tagaaaaaaa taatttagag acatttaaaa aattcacgct tctaatactg
     8401 gtacctgtct caatagtact tgtgttaata ctatggggaa gtgcctcaca ggcactgttg
     8461 ctggcattta tatgctgtct gattctttat atatctgatg taaaaagaag tcatctcctt
     8521 aaaagtgcag gtataattgg tgccggacta attctgattc tattactcca cttcagcttt
     8581 gggatattcg gacgcataga tacagcaatc agccgtataa aaaacaatga tacggaacaa
     8641 ctaaacaata aaaacctctc accagaagac agaaaagaac tggaaaacag cacatttcaa
     8701 aaaagaatgg ctaaagtggc cgtctcttca gcagggataa taggtaaagg gccgggaaaa
     8761 agtactcaaa gatatgtatt gcctcaccct tactctgatt ttatatatgc aataatagtc
     8821 gaggaatacg gtctgatagg aggtgttatt gtacttatgc tttatctgtg gctgctgttc
     8881 agatgtatga ctctcgccta ttcctgtacg aaaccattct cgcggatagt tgtcattggc
     8941 cttggcctta tgataacatt tcaggcacta ttgcatatga tggtaaatgt tactctgatc
     9001 ccggtaacgg gtcatacttt accattagta agccttggag gaacatctat tattattatg
     9061 ggtggagctt ttggaataat tctctctata agcagaacta ttgagataac caaagaaaat
     9121 aacacaaaaa aaattgaagt aaatgtcgga gaagagaatt ataattagtg gagggggaac
     9181 agggggtcat atatttcctg ccatctccat cgccaacagt cttaaaaaga ttgatcctga
     9241 gtgtgagatt ctttttgttg gtgcactcgg aagaatggag atggaaaaag tacctgcagc
     9301 aggctataag attgtcggcc ttccagttgc aggtctcaaa agggagttgt ctgtcgaaaa
     9361 tctaaaattg ccttttaaaa tttttaaaag tctttgtcag gcaagaaaaa tagtttacga
     9421 ctttgatccg gacgtagcag tgggagtagg aggatatgcg agtggtccgg tactctggat
     9481 ggcaagtaga atgaatattc ctatcctgat acaggaacaa aactcctatg caggccttac
     9541 caaccggatt cttggaaaaa aagccaaaaa aatatgcgta gcctatgaag gaatggaaaa
     9601 gttttttaaa gctgagaaaa taattctcac cgggaatccg gttcgtgaag ggatagttct
     9661 cataaaccca caacaaaaag aagaagctta tcgatatttt gaactggacc ctcaaaagaa
     9721 aacaattctt attattggag gtagtcttgg cgccagcacc ctgaataatt gtgcaaaaga
     9781 atggataaga aaaggcactc aggaaaatat tcagctaata tggcaatatg gaagttttta
     9841 taaaaatgag atacgtgact ttataagtgc caatccaaaa ccaaatgtta aagcatttga
     9901 atttatcaag agaatggatt atgcatatgc aatagcagat gtagtaattt caagagcagg
     9961 agcgggaaca atctcagaat tggcagtagc cggcaaggca tcggtgctga taccatctcc
    10021 aaatgttgtt aaagaccatc agactcacaa tgcaatggct ctagtcagaa agaatgccgc
    10081 tataatgatt cctgacgatg aagctgtaga aaaagcattt aaggcggccg tggatctggc
    10141 agaagatgaa aataaaagaa aagagctcga gactcgaata tccggaatgg ctataagaaa
    10201 ttcagctgaa attattgccc gtgaggtact taaactggca gagaaatgaa ctttgtatat
    10261 ttaataggaa taggaggaat aggcatgagc gctctcgcaa ggtattacaa ccacgcaggg
    10321 gcaattgttg ccggatatga tcgcaccccc agcaaactta cttcagaact tgaaaaagag
    10381 ggaatcagaa ttcattatga agacgatatt gcactaatcc cacctgagtt cacaagttac
    10441 aaggagaaaa cgttagtgat ttatactcct gccatacctg acaatcacag cgagtatcag
    10501 tattttctga aaaacggata taacatcgta aaacgttcac gggcactcgg aaatattgcg
    10561 tctcaaaaac gctgccttgc aatatcgggc actcacggta aaactactac ttccacatta
    10621 ctggctcata ttttaaccca atcaggcaag ggctgtactg catttttagg aggcatctca
    10681 aaaaattatc actccaatct tcttcttgac aaaagagatt accttgttgc agaggcagat
    10741 gaatttgatc gctctttttt acaactttat cctgaaatgg cagtcataac ttcgacagat
    10801 gccgatcatc tcgatattta cagtacccat aacaaaataa aggaggcttt tttagaattt
    10861 gcttcacaag ttaagcccga cggagcactt atcgtcaaaa aaggcactga catagacctc
    10921 agctctgtaa aagcagagag agtatatgaa tattcctatg acattccctg cgatttctat
    10981 gcgtcgtcaa tcaaagtatt ggaagatggc ttttttaatt ttgatcttaa ctatcctggt
    11041 ggcgttataa aaaattgcac tgtaggaata ccaggatgga taaacattga aaattccatt
    11101 gccgcctctt cgattgctct tctcgagggc atctcccccg aagatttaaa aaacagtctc
    11161 aaaacgtttg caggagttga acgtagactt gacataagag taaagaacga aaaatgttcc
    11221 tatgtcgacg attatgctca ccatccaaaa gaattatctt ctgcaataag ctctttgaga
    11281 gaaatctttg ctggaagaaa gattacagga attttccagc cgcatctcta tacaagaaca
    11341 agggactttg cggaagaatt tgcaaaaagt ctcagtcttc tggatgaact aattctgctc
    11401 gacatttatc ctgcaagaga agaaccaata gaaggagtaa catccgaaat aatatataaa
    11461 aacgtcagcc tcgacagtaa aataatgttg cacaaggaag aactaataaa ctacttggca
    11521 aagatagata tagatgtttt agtcacattc ggcgcaggag acattgaccg tttcgtaaat
    11581 ccaataacac aattagtttc tgcaaaatca aaatgctaaa aaaaactctc ataattattc
    11641 tttatctgct tctgaccgga gtactttgtt gctatttcat cttctcatct aaaataacaa
    11701 aagagggcaa aatgcacgaa atgtgccatc aaataaaagt tgtaatactt gacagtgccg
    11761 agataagatt cgtttcaaaa acagagattg cagatttgat cgtcgataat ggtataactc
    11821 caggagagag caagttacgc catattaaca attaccagat agaacaactt ctaaataaaa
    11881 gaactgccgt caaaaaatct gaagtaagct atacgagaga cggtattttg agagttataa
    11941 ttttacaaag aaaaccgatt ctcagacttc agacagaaaa tggaggtttt tacatggatg
    12001 aaaacgctta tgtatttcca ttaataaaag gattctcccc ttttgttcct gttgtatctg
    12061 gagagattcc gcttgaaatt cctccaaaat acaacggata tgtcaaaaac aacgaacagt
    12121 gggctaattc tcttaaggat ttatgtttat atatcttaaa aaacgaatcc tgggatgcat
    12181 taattgaaca gatatatgtt gacagcagag gtattttaag cttcatcccc cgtgatggag
    12241 attttgaaat aatatttgga aatatggaca agatagaata taagttcaaa aaattgtatg
    12301 ccttttataa ggatatactt ccaaacgtcg gtccggagaa atactgcaaa attaaccttc
    12361 agtttgacaa acagatagta tgtaaaaaaa caaataaaaa cattgagata tgaaaaaact
    12421 tgtcgcagcc gtagaccttg ggacaacaaa ggtcgtctct gttgtaggtg aaaaaagtga
    12481 atccggttac aggattgtgg cattgaaaga agccccttca aaaggggtaa tgcgtggtga
    12541 agttgtcaat attcaaaacg ttctggaatc tcttaaaccg actctcgaag aaataagagc
    12601 agagggcatt gaaattagtg aagtatacgt cggtatagca gggcagaata taaaatgtaa
    12661 ctcggcaaga atcagcagga acagaagccg gtcatcagaa tggattacaa acagggaagt
    12721 cgaagagatg gagagagaaa tgttcaattc aagagtcaac gctggagaaa aaattcttca
    12781 tgttattcct caatcatata atgtcgacga caatgttggt gtaaaagagg cagtaggcat
    12841 gactgggact cgtattgaag gagattacaa gctttttata ggcaagacca attcctttga
    12901 gcacagtaaa attgttatcg aaagagcagg cttaaagtta aaagatataa tcctggaacc
    12961 ggtcgcatcg gccaaggcag ttctcacaga agacgaaatg gaacttggtg ttgcaatggt
    13021 tgacatggga ggagggacaa cagaccttct tataatacag gataacatta tcaggcatac
    13081 tgcagtaata cctttcggcg ggaattccat tactgaagac ctccgtcaag gatgtggcgt
    13141 atctctccga aatgctgaac agatgaagat ccaatatggt tcttgctaca gtgaccgtgc
    13201 tccggataat aagactgtaa taataccagg tcttggtggg cgtgattcga gagaggtcgc
//
LOCUS       DAOTQL010000035        13164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4413_length_13164_cov_5.240827, whole genome shotgun sequence.
ACCESSION   DAOTQL010000035 DAOTQL010000000
VERSION     DAOTQL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_13164_cov_5.240827"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="P5293_03465"
     CDS             complement(<1..731)
                     /locus_tag="P5293_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRR48991.1"
                     /translation="MITKELISPKSIVVVGGSDETQKPGGAVLRNLRESNYKGKLYVV
                     NPKSDFVQGIKCHKRVEELPQVDLAILAIAAKMCPDSVEILCSKKMCKAVIILSAGFH
                     EDGPEGAILEEKIVEIVNRNGASLIGPNCIGVMTPNYTGIFTKPNPPLSENGVDLISG
                     SGATVVFILETALQLGVRVSSIFSVGNSAQTGIEDVLEYLDETYIHKKSAPVKLLYIE
                     SISYPEKLLKHAVSLIKKGAKIAAI"
     gene            916..2169
                     /locus_tag="P5293_03470"
     CDS             916..2169
                     /locus_tag="P5293_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRR48992.1"
                     /translation="MSTLLIKNVNNNNKITDIFIKDNRIEKISEHIDKGADRIIEGKE
                     KYVIPGFINMHTHAAMTLLRGIGEDMSLMDWLQNVIWPTEQKLDEELVYWGTKLACVE
                     MIRSGTTCYNDQYWYLPAAQRATAEMKLRSVHSYVILDLMDKSKSEKMKDECKKMYDF
                     SRDWPERNIFAVGIHAPYSVSEDMIRWASDFARERGLLVHLHLSETESENLDSLKKYG
                     MSPTKYLDSLGALGPETLAAHCLWIDDEDIEILAKRRVKIIHNINSNLKIASGYRFKY
                     REFKEAGIIVSLGTDGCASSNNLDMLETMKTTALVQKAWRKDPTVLPMEELLSLATLN
                     GGLSLGLETGIIKEGYLADLSLVDVNSVAFTPNINFLANLIYSANSSCIDTVICDGNI
                     LMENKKIDEEEEIIQMVNKLYKKLL"
     gene            2179..3000
                     /locus_tag="P5293_03475"
     CDS             2179..3000
                     /locus_tag="P5293_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase I, inosine and
                     guanosine-specific"
                     /protein_id="HRR48993.1"
                     /translation="MEYIQQIEEATAFISDKLSGRKPKVGIILGSGLGGLAEIIEEKC
                     IIPYTQIPHFPQSTAIGHKGNLIGGILGGKYVLAMQGRFHYYEGYSMNQVTLPVRVMK
                     KLGISSLFVSNAAGGVNHDFKVGDLMIIRDHINLMPNPLIGKNLDEFGPRFPDMTRPY
                     DLELIRKAERIASVLDITLKKGVYLAGTGPTYETPAEYAFYGIVGADAVGMSTVPEVI
                     VARHCNIPVFGISVITNEAHDFTEDFVNDGEEVIAAANRAADKMTQLFVSLISDL"
     gene            2997..3620
                     /locus_tag="P5293_03480"
     CDS             2997..3620
                     /locus_tag="P5293_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRR48994.1"
                     /translation="MIRNIVFDLGGVIITLDRQACVDSFKKLGFKDFDDILNDFVQDG
                     FFLEFEKGAIDAARFREIICDHIGHIVKDYEIDNAMGDFLSDIPVKRFETLLRLKKSY
                     NVFLLSNTNPIAMSKVRDIFSAYGIDMNDCFDKMYLSYQMKMVKPNNEIFLKMMEDSR
                     IKAEESLFIDDGEMNINTANSLGFKTLHLKKGIDLDSEIEKFLNEQK"
     gene            complement(3669..5339)
                     /locus_tag="P5293_03485"
     CDS             complement(3669..5339)
                     /locus_tag="P5293_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRR48995.1"
                     /translation="MKKGLLILTLSVIMASCSKNDEKRLQEIITSTEDTIRPLAKMAA
                     LAYWNGTITGDPAEFKKYSDATIQITKVFSNPDTFAELKTIKERGKVKDSLLTRQLTV
                     LYNQFLANQADTSLLNAIINKTSALELKYAEYRANFKGKPITDNEVESILKTSTDSKV
                     LEEVWRAHKEIGPVVANDIIELVKMRNKVATSLGFDNYHSMTLELSGQDPKEISAIFD
                     ELDSLTKDAFVKVKGEMDSVLSLRYKTASADLMPWHYQGRYFQESPNIYPVNLDSYYK
                     GKNLEKLTSNFYNSIGIDITKVMQNSDLYEKPKKNQHAYCTDIDNEGDIRVLCNIRDN
                     EQWMGTMLHEFGHAAYSLGHDNPNNPYFLRSAAHTFTTEAIAMLFERLSRNPEWMKQA
                     LGLTQEEKENISKDCEKAARLKQLVFSRWVQVVYRFEKSMYENPDQDLNKLWWDLVEK
                     YQMLKKPVDRNSPDWATKIHIALYPCYYHNYQLGELLASQIHYYIVDSVTKSGDYKNE
                     CYIGNKDIGNWMKEKIFNVGMRYEWNDMIERATGEKLTAKYFAKQFID"
     gene            complement(5343..6941)
                     /gene="pckA"
                     /locus_tag="P5293_03490"
     CDS             complement(5343..6941)
                     /gene="pckA"
                     /locus_tag="P5293_03490"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRR48996.1"
                     /translation="MTSIDLTKYGIRNVKNVLHNPSYEELFQAEVSEKNEGYEKGILT
                     KTGAVCVKTGIFTGRSPKDKYIVKDATSDQHMWWDGNINQPVSPEIYNECKALVQKQM
                     SDKETIYVMDAFCGTNEDTRLKVRFIVEVAWQAHFVKNMFIRPSRYELEHFGEPDFVF
                     INGSKVTNPKWKEQGLHSDVFVMFNLTERIAICGGTWYGGEMKKGIFSMMNYFLPLKG
                     IASMHCSANVGENGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGIFNYEGGC
                     YAKTINLSQENEPDIWDAIRRDALLENVTIREDGTPDYSDASVTENTRVSYPIYHINK
                     IVLPSKAGHAKKIIYLSADAFGVLPPVSILDRDQAQYHFLCGYTSKLAGTERGITEPV
                     PSFSPAFGEAFLTLHPTVYASTLAKKMDEHGAKAYLVNTGWNGTGKRISIKDTRAIID
                     SIIDGSIEKTEKKHIPILNLTVPVKLDNVSDILDPRDSYADKNVWEEKARSLAAKYIK
                     NFEQYCDNDAAKKLVKSGPQLNNE"
     gene            7050..8954
                     /locus_tag="P5293_03495"
     CDS             7050..8954
                     /locus_tag="P5293_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRR48997.1"
                     /translation="MVRENEILKKYWGYETFRPLQREIIESALEGKDTLALLPTGGGK
                     SICFQIPALMVEGICLVVSPLIALMKDQVENLKKRGIKALQVHSGMTRDEIDYTLDNA
                     IYGDYKFLYLSPERLQSEIVRVRISNMNVSFLVVDEAHCISQWGYDFRPDYLLIKDIR
                     DIVGNVPVIALTATATPEVANDIMKQLNFSKSNIIRSGFERKNLSYVVREVEDKNGQL
                     LKMVFSIQGSGIVYVRERKKADEISAFLNAQGISADSYHAGYGSEERAKKQERWMKGD
                     TRIIVATNAFGMGIDKADVRFVCHFDLPESIESYFQEAGRAGRDEKKAYAVLLWNKHD
                     IKRLNRIISISFPSFEYLTEVYQRLFNYIGYEFGTGKGDVIRFDIGDFALKAKLHLVS
                     AYHAIKYIESEGYWELTEEIETPSRIMFTVSRDELYKVQLKSTYLDSFIKILMRLYTS
                     IFSTYVPIDEEYIARVSRNSKANVTANLITLSRMHVISYIPSVISPLLILKVERLENR
                     GLLFSYENYKKRKANFEKRVKSIIDYASSKEQCRSKMLLEYFGEIGAEKCGICDVCLK
                     RKDDFTRLSIKGDNTVEKLIRQQLSAKSLSMEEILLNFPEETDLIIDIMRDMIDRGII
                     KEVSGKYSVI"
     gene            complement(8943..9539)
                     /locus_tag="P5293_03500"
     CDS             complement(8943..9539)
                     /locus_tag="P5293_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRR48998.1"
                     /translation="MLKTLLEGKKIILASSSPRRKQLLDGLDIEFTVEENGNMSEQFD
                     PNMDLYKIPEHIALLKSKGFKRKLNYNELLVTADTMVICNNEIMGKPKDRSDAFKMLQ
                     KLSGNKHTVLTGVCMRNNYKIRSFTSSTDVFFRHIKDEEIEHYIDNYLPYDKAGAYGA
                     QDWIGLVAIERIDGSFFNVMGLPVQKLYTEMESFLSDN"
     gene            complement(9551..10081)
                     /locus_tag="P5293_03505"
     CDS             complement(9551..10081)
                     /locus_tag="P5293_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRR48999.1"
                     /translation="MSNYKYKLSSIKAFAFDVDGVFTDGNVLVTDSGDLLRSHNAKDG
                     FAVRMALLNGYPVAIITGGISNSILKRFYQIGIDKEDIYLDSHHKIQDFIDFCKRHSF
                     DPEEVLFMGDDIPDIGILKECGLPTCPSDAVNEVKEVCEYISLYPGGKGCVRDVIEQV
                     LKIQDNWNPSSEAYSG"
     gene            complement(10101..10940)
                     /locus_tag="P5293_03510"
     CDS             complement(10101..10940)
                     /locus_tag="P5293_03510"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRR49000.1"
                     /translation="MQNLINLAYNSFMYRISIWGTGNVAYRLSIELKAAGHSIPFICG
                     RNVTSGKKLVRILNKEENNPHKVEEATCFTNDFHSLKDSDIIILAVSDDVIQTLAKEF
                     ATITFKDKKIPLLIHTSGATSIEALECYPFTGVLYPLMTLSKIKPVNFKLVPFLIESY
                     SPEGEKILKDIVLSLGSEYMISTSDERLKMHLAAVYVSNFVNYMIGLAFDLSKPNQEL
                     LMPLAIETVRKAFLYEHPSIVQTGPAKRGDIQTISKHLKLLEGMSEHRYVYEMLSKLI
                     SKQ"
     gene            complement(10963..12690)
                     /locus_tag="P5293_03515"
     CDS             complement(10963..12690)
                     /locus_tag="P5293_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRR49001.1"
                     /translation="MANFYRDNKDIQFQFSHPLMKKIIQLREQDFKDFGKFDYAPCSF
                     EDALDSYDRVMDILGEICGDVIAQNAEEVDKVGPHHENGHVTYAPGTQQNHEVLTQAG
                     LYGMNLPREYGGLNFSMVPYIMSAEIVGRADAGFSNIWGLQDCADTIYEFANQEIRDE
                     FLHRINKGETCSMDLTEPDAGSDLQAVMLKATLDEKSGMWKLNGVKRFITNGDAQIKL
                     VLARSEENTTDGRGLSYFVHDQKWGGVTVRRIENKLGIKGSPTCELVFNNAPAKLVGE
                     RRMGLIKYVMSLMNGARLGVAAQAVGISEAAYREALKFAKERRQFGKPIIQFPAVYEM
                     LSIIKARLQASRALLYETTRFVDVYKAYNMIAETRKLTLEEKNEAKQYQRLADMFTPM
                     VKMMASEYSNQNAYDALQVHGGSGFMKDYPIERIYRDARITTIYEGTSQLQVVAAQRY
                     VTNGAYLNQIKEYEKIEIKSEFTQLKKILVAMTEQYQQAVAIVSGGDTEFIDFHARRL
                     VEMASHIIMSYLLIIDAQSDRRFRDSAIIFTNKSKAWNDERYSYIADFAPCDLNQFLA
                     VKEEYLPES"
     gene            complement(12692..>13164)
                     /locus_tag="P5293_03520"
     CDS             complement(12692..>13164)
                     /locus_tag="P5293_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRR49002.1"
                     /translation="GVINQLDVNLILNDSDFVVEIIRRDIQEKKIDIKGASIIVSGGF
                     GVGSKENFNQLFELAHLLGGEVGASRAAVDAGFADHARQVGQTGVTVRPKLYIAVGIS
                     GQIQHVAGMDQSAMIISINNDPNAPINNIADYAIIGDLNEVLPKIIKSYKKNIK"
BASE COUNT         4039 a         2362 c         2420 g         4343 t
ORIGIN      
        1 ttaattgcag caatttttgc tccttttttg ataagtgaaa cggcgtgttt taacaatttc
       61 tctggatagc tgatgctctc aatataaaga agcttaacag gagcgctttt tttatgaata
      121 tatgtttcgt caaggtattc gagtacatct tctatacctg tctgggcact gttgccaaca
      181 gaaaaaattg atgagactct tacgccaagc tggagggctg tttcgagaat gaaaacaacg
      241 gtagcaccgg aaccggaaat aaggtcaact ccattttctg aaagaggggg atttggtttt
      301 gtgaatatgc cagtatagtt gggagtcata acgcctatac agtttggccc tataagagat
      361 gcaccgtttc tgttgactat ttcaacaatt ttttcttcta aaattgctcc ttccggccca
      421 tcttcatgaa accctgccga caatattatg acagctttgc acattttttt gctgcacaat
      481 atctcgacac tgtccggaca cattttggct gcaatggcta aaattgctaa atctacctga
      541 ggcaactctt cgactctttt atggcattta attccctgta cgaagtctga ctttggatta
      601 accacatata gttttccttt ataattggat tctctgagat ttcttagaac tgcaccgccg
      661 ggcttttgtg tctcatccga accacctaca acaacaatgc ttttaggaga tattaattct
      721 tttgttatca tatgttaata aatttgtcac aaaggtaata ataagaaaga gttattaatt
      781 aacaggatgc aattataaat aaaaatccat tttgacttat atatttatat ttcaaaacga
      841 atatgacatt aaaaaaatat ttttgagtta gagtaaaaaa actgattatt tttacattac
      901 taataacaaa gaaacatgtc tacactatta attaaaaatg tcaataacaa caataagata
      961 acggatatat ttataaagga taatcgtatt gaaaagattt cggagcatat tgacaaaggt
     1021 gctgacagaa taattgaagg gaaagaaaaa tatgtaattc ccgggtttat taatatgcac
     1081 acacatgcag ctatgactct tctgcgcggg attggtgagg atatgtcttt aatggactgg
     1141 ttgcagaatg ttatctggcc taccgaacaa aagcttgatg aagaacttgt atattggggg
     1201 acaaagttag cttgtgtgga aatgataaga tcaggaacaa cctgctacaa tgatcaatat
     1261 tggtatcttc ctgctgctca aagagcaact gccgaaatga aactcagatc tgttcattcg
     1321 tacgttattt tggatttaat ggataagtct aaatctgaaa aaatgaagga tgaatgtaaa
     1381 aagatgtatg atttttccag ggattggcct gaaagaaaca tttttgctgt aggcatacac
     1441 gctccctaca gcgtttctga agatatgata agatgggcaa gtgattttgc gagagaaaga
     1501 gggttgcttg ttcatctcca cctttctgaa acagaatctg aaaatttgga ttcacttaaa
     1561 aagtatggaa tgtctcctac aaaatatctg gattctcttg gtgcattggg acctgagact
     1621 cttgctgccc attgtctttg gatagacgat gaagatatcg aaatacttgc aaaaagaaga
     1681 gttaagataa ttcataacat aaattccaat ttaaaaattg cttctggcta cagattcaag
     1741 tatagagaat tcaaagaagc cgggattatc gtatctttag gaacagatgg ttgtgcttct
     1801 tcaaataatc ttgacatgct tgagaccatg aaaactacag cacttgtcca gaaagcctgg
     1861 cggaaggatc caacagtgct tccgatggag gaacttttat ctctggcaac tctcaatggc
     1921 gggctttctc ttggtcttga aactggaata ataaaagagg gatatcttgc tgatctttca
     1981 cttgtggatg ttaatagcgt tgcatttact ccaaatatca attttttggc taatctaata
     2041 tactccgcta acagctcatg tattgacact gtaatatgtg acggaaatat tttaatggag
     2101 aataaaaaaa ttgatgagga agaggaaatt attcaaatgg ttaacaaatt gtataaaaaa
     2161 ctattataga ttcatattat ggaatatatt caacaaattg aagaggcaac agcctttatt
     2221 agtgataaac tttctggcag gaaacctaaa gtcggtatta ttcttggcag tggattggga
     2281 ggattagcag aaataattga agagaaatgt ataattccct atacacaaat tccacatttt
     2341 ccccagtcca cggccatagg tcataaaggg aacttgattg gtggtattct tggtggcaaa
     2401 tatgtacttg ctatgcaggg aaggttccat tattacgaag gatacagcat gaatcaggtt
     2461 acacttccgg taagagttat gaaaaaactt ggaatctctt ctcttttcgt ttcaaatgcg
     2521 gcaggaggtg taaaccatga ttttaaagtt ggtgatctta tgataattag agatcacatc
     2581 aatttaatgc ctaatccgtt gattggaaag aatttagatg aatttggtcc acgttttcct
     2641 gatatgacga ggccatatga cctggaacta ataagaaaag cagaaagaat tgcatccgtt
     2701 ttggacatta ctttaaaaaa gggggtttac cttgcaggta caggtccaac ttatgagact
     2761 cctgctgaat atgctttcta tggcatagta ggtgctgatg cagtaggcat gtctactgtt
     2821 cctgaagtaa ttgttgcaag acattgcaac ataccggttt ttgggatatc tgtaattaca
     2881 aacgaggctc atgattttac agaagatttt gtaaatgatg gggaagaagt aattgctgct
     2941 gccaacaggg ccgctgataa gatgacccaa ttgtttgtct ccttaatatc ggatttgtga
     3001 ttagaaatat tgtttttgat cttggaggag taataattac tcttgacaga caggcctgcg
     3061 tagattcttt taagaagtta ggcttcaagg attttgatga tattctgaat gattttgttc
     3121 aggatggttt ctttcttgaa tttgaaaaag gagctattga tgcagcaagg tttagagaaa
     3181 taatttgcga tcatatagga cacattgtca aggattatga aattgacaat gcaatgggtg
     3241 actttctttc tgatattcct gtcaaaaggt ttgaaacgtt gttgagactt aaaaagagtt
     3301 acaatgtttt tttactcagc aataccaatc ccatagcaat gagtaaagtt agagatattt
     3361 tttcagctta tgggattgat atgaacgact gctttgataa aatgtatctt tcatatcaga
     3421 tgaagatggt taaaccaaac aacgaaatat ttttgaaaat gatggaggat tcaagaatta
     3481 aggcagaaga atctcttttt attgatgatg gagaaatgaa tattaacaca gctaattcac
     3541 tgggtttcaa aactctacat ttaaaaaagg ggatagatct cgatagcgag attgaaaagt
     3601 ttttgaatga acaaaaataa atcagggtgt caaagcaata atttccgaca ccctgatact
     3661 atattgaatt aatcaataaa ttgttttgca aagtattttg cagtaagttt ttctcctgtt
     3721 gctctctcaa tcatgtcatt ccattcgtat ctcattccta cattaaaaat cttctctttc
     3781 atccaattgc caatatcttt attgccaatg tagcattcat ttttgtagtc tcctgattta
     3841 gtgacgctat caactatata gtaatgaatt tgagaagcaa gcagttctcc taactggtaa
     3901 ttgtgataat aacaaggata aagtgcaatg tgtatcttag ttgcccagtc aggagagttt
     3961 ctatctaccg gttttttgag catctgatat ttttcaacca aatcccacca caatttgtta
     4021 agatcctgat ctggattttc gtacatcgat ttttcaaatc tgtatacaac ttgaacccac
     4081 cggctgaaaa cgagctgttt taatctggca gctttttcac aatcttttga aatattctct
     4141 ttttcttcct gagtaagacc aagtgcctgt ttcatccatt cagggtttcg agacaggcgt
     4201 tcaaatagca ttgctattgc ttctgtagta aatgtatgag cagcacttct cagaaaataa
     4261 ggattatttg gattatcatg tcccaaagag taggcagcat gtccaaattc atgaagcatc
     4321 gttcccatcc actgttcgtt gtctcttatg ttacatagaa ctctgatatc gccttcgttg
     4381 tcaatatcag tacagtatgc atgttgattt ttctttggtt tttcgtacag atcgctgttt
     4441 tgcattactt tagtaatgtc aattcctatg ctgttataaa aattcgaggt taatttttcc
     4501 agatttttac ccttatagta cgagtcaaga ttaaccgggt aaatatttgg agattcttga
     4561 aaatatcttc cttgatagtg ccaaggcata aggtctgccg aagcagtttt atatcgtaaa
     4621 gaaagtacgg agtccatttc tcctttgact ttcacaaaag catcctttgt aagagaatct
     4681 aattcatcaa aaatagcaga aatttcttta gggtcttgtc ccgagagttc caaagtcatg
     4741 ctgtgatagt tgtcaaatcc aagagatgtc gcaactttat tgcgcatctt tacaagttct
     4801 ataatatcat tggcaacaac cggtccaatc tccttgtgcg ctctccacac ttcctcaaga
     4861 accttactgt cagtagaagt tttaagaata ctctcaacct cattgtctgt tattggtttg
     4921 cctttgaagt tagctctgta ctctgcatat ttaagttcaa gggcagaagt tttgttgatt
     4981 attgcattta gaagagaagt atctgcctgg tttgcaagaa actgattata aagcacagtg
     5041 agttgtctgg tcaatagcga atctttaact ttcccccgtt cctttattgt ttttaattca
     5101 gcaaaagtat caggatttga gaacacttta gttatttgta ttgtggcatc cgaatatttt
     5161 ttaaactctg cagggtctcc tgttattgtt ccgttccaat aagcaagagc tgccattttg
     5221 gctaatgggc ggatagtatc ttcagtgctt gtaataatct cctgaagcct cttctcatcg
     5281 ttttttgagc aactcgccat aattactgac agtgtcaaaa ttaacagtcc ctttttcata
     5341 ttttattcgt tattgagttg tgggccagat ttaactaatt tttttgcggc atcattatca
     5401 caatattgtt cgaaattttt tatgtatttg gcagctaatg atctggcttt ttcttcccaa
     5461 acgtttttat cagcataaga atctcttgga tcaagaatgt ctgaaacatt gtccagcttt
     5521 accggaacag tgagattgag aataggaata tgtttttttt cagtcttttc aatagatcca
     5581 tcaataatag aatcaataat tgctcgggta tccttaattg aaatcctctt tcctgtacca
     5641 ttccatcctg tatttacaag ataagcctta gctccgtgtt cgtccatttt tttggcaaga
     5701 gtagatgcat aaacagttgg atgtaaggtt aaaaaggcct caccaaaagc aggagagaac
     5761 gatggaacag gttctgtaat tcctctttca gtaccggcta atttagaagt gtaaccgcac
     5821 agaaaatggt attgagcctg atctctgtcc aaaattgata caggagggag tactccaaag
     5881 gcatctgcgg aaagatagat aatttttttt gcgtgacctg cttttgaagg aagaacaatc
     5941 ttattaatat gataaatagg ataagatacc cgggtgtttt cagtaacaga agcgtcagaa
     6001 taatctggtg tgccatcctc tctaattgtc acattttcca gaagtgcatc tcttctaata
     6061 gcatcccata tatccggttc attttcctga ctaagattta tcgttttagc ataacagcca
     6121 ccttcatagt taaatatgcc atgatcatcc cagccatgtt cgtcatcacc tattaaatag
     6181 cgcttagggt cagctgaaag agtagtttta cctgttcctg aaagaccgaa gaaaatagcc
     6241 acatcaccat tttcaccaac attggctgaa cagtgcattg aagcgatgcc tttgagtgga
     6301 agaaagtagt tcatcatgga gaaaattccc tttttcatct cacctccata ccatgttcca
     6361 ccacatatag ctattctttc agtaagattg aacatgacaa atacgtcaga atgaagtcct
     6421 tgctctttcc atttaggatt tgttactttg gagccgttaa tgaaaacgaa gtctggttct
     6481 ccaaaatgct caagttcata tctggatggg cgaataaaca tgtttttaac aaagtgtgcc
     6541 tgccatgcta cctccactat aaatctgact tttagtctgg tatcttcatt agtgccacag
     6601 aaagcatcca tgacatatat tgtctcctta tcagacatct gtttctgaac aagagccttg
     6661 cattcgttat atatttcagg agagaccggt tggttaatgt ttccatccca ccacatatgc
     6721 tgatcgctgg tagcatcttt tacaatatat ttatctttag gtgaacgacc tgtgaagata
     6781 cctgttttaa cacatactgc gcctgtttta gtaagaatac ccttttcata tccttcattt
     6841 ttttctgaaa cttcggcctg aaaaagttcc tcgtaagagg gattgtgtaa cacattcttt
     6901 acgtttctga taccatactt agtcaaatca atacttgtca tagagcattt tttaaattat
     6961 tggttaatat tactatccgt ttctttagaa gatgtaaaat tagtaaatta gcaacaaaaa
     7021 tcaaaccata ttgttaatgt tgtgaatgta tggtaagaga aaatgagatt ctaaaaaagt
     7081 actggggcta tgaaacattt cgtcctcttc aaagagaaat aattgaatct gctcttgaag
     7141 ggaaggacac actggctctt cttcctacag gtggaggtaa gtctatttgc tttcagattc
     7201 ctgcattgat ggttgaaggt atttgcttgg ttgtaagtcc tctgatagca ttaatgaaag
     7261 atcaggttga aaatttgaag aaaaggggga taaaagcttt gcaggttcat tcagggatga
     7321 caagagatga gatagattat acgttagaca acgcaatata tggagattac aagtttctct
     7381 atctatctcc tgaaaggctt caaagtgaga tagtcagggt caggatatct aacatgaatg
     7441 ttagttttct tgttgtggat gaagctcact gtatttcaca gtggggttac gattttcgtc
     7501 ccgactatct gttaataaaa gatatcagag atattgtcgg gaatgttcct gttattgctc
     7561 taactgctac agcaacaccc gaagttgcca acgatataat gaaacagtta aatttttcta
     7621 aatccaatat tatcagatct ggctttgaaa ggaaaaatct ctcttatgta gttagggagg
     7681 tcgaagataa aaatggtcaa ttacttaaaa tggttttttc tattcaaggt tcaggcattg
     7741 tatatgtcag agaaagaaaa aaagcagatg agatttcggc atttcttaat gctcaaggaa
     7801 ttagtgccga ttcgtatcat gccggttatg gatctgaaga gagggctaaa aaacaggaaa
     7861 gatggatgaa gggagatacc agaatcattg ttgccacaaa tgcctttgga atgggtattg
     7921 ataaagcaga tgttagattt gtatgtcatt ttgatcttcc tgaatccatt gaatcatatt
     7981 tccaggaagc tggtagagca ggcagggatg aaaaaaaggc gtacgctgtt ttattgtgga
     8041 acaagcatga tattaaaagg ctgaatagaa tcatttcaat atcatttcct tcatttgaat
     8101 atttgactga agtttatcag cgccttttca attatattgg atatgaattt ggtacgggaa
     8161 aaggtgacgt gatacgattc gacattggcg attttgcact taaagccaag ctacatttag
     8221 tttcagcata tcatgcgata aaatatattg agagtgaagg ctactgggaa ttgacagaag
     8281 agattgaaac tccttccaga attatgttta cagtatcaag agatgaactt tataaagttc
     8341 aacttaaaag tacttatctt gattctttca taaaaattct catgaggctt tacacttcca
     8401 ttttttcaac ctatgttcct atagatgaag aatatattgc aagagtttct agaaacagca
     8461 aggcaaatgt tactgccaat cttattactt tgtctagaat gcatgtcatt agttatatcc
     8521 cttctgtaat atctcctctt ttaattttaa aggttgagag acttgagaac aggggactgc
     8581 ttttttccta tgaaaattat aaaaaaagaa aagcaaattt tgaaaaaaga gtaaagtcca
     8641 ttattgacta tgcatcttcc aaagaacaat gccggtccaa aatgttgctt gaatattttg
     8701 gtgaaattgg agctgaaaaa tgtggcattt gtgatgtttg tctcaaaaga aaggacgatt
     8761 tcacaagact ctccataaaa ggagacaata ctgttgagaa attgatcaga caacaattat
     8821 ctgcaaaatc tctatcaatg gaagagatat tattaaattt tccggaagag acagatctca
     8881 ttattgatat tatgagagac atgattgaca gaggaataat aaaagaagtt tcaggaaagt
     8941 attcagttat ctgataggaa actttccatt tctgtataaa gtttctgaac aggtaaaccc
     9001 attacattaa agaaagatcc atcaatacgt tcaatagcaa ctagtccgat ccagtcctgt
     9061 gctccatatg ctcccgcctt atcatatgga agataattgt cgatgtagtg ttctatctct
     9121 tcatctttta tatgcctgaa aaagacatcc gttgaggatg taaatgatct aatcttataa
     9181 ttatttctca tgcatactcc tgtcagaact gtgtgcttgt tacccgaaag tttttgcaac
     9241 attttgaaag catctgatct gtccttgggc tttcccataa tctcattatt gcaaatgacc
     9301 attgtatctg cagtgacaag taactcgtta taattcaact ttcttttgaa tccttttgat
     9361 ttgagtaagg caatatgttc aggtatctta tataaatcca tatttggatc gaattgttct
     9421 gacatattgc cattttcctc aacagtaaat tcgatatcca aaccgtccaa caactgtttc
     9481 ctgcgtggag aagaggatgc taaaataatt tttttgcctt caagcagtgt tttaagcatt
     9541 ttaaacctcc tcaacctgaa tatgcttcag aacttggatt ccaattatct tgaatcttca
     9601 aaacctgttc tatcacatct cttacgcatc cttttccgcc agggtaaagt gagatatatt
     9661 cacaaacttc cttaacttca ttaactgcat cagatggaca agtaggtaaa ccgcactctt
     9721 taagtatccc aatgtcagga atatcatctc ccataaaaag tacttcttca ggatcaaaag
     9781 aatgcctttt gcaaaaatca ataaaatcct gaattttatg atgtgagtca agataaatat
     9841 cctctttgtc aattcctatt tgataaaaac gtttgagaat actattggaa attccaccgg
     9901 taatgatggc aacaggataa ccattgagca acgccattct tactgcaaat ccatctttgg
     9961 cattgtgact tctaagtagg tccccactat cagttacaag cacattgccg tcagtgaata
    10021 caccatctac gtcgaaggca aaagctttaa tagaagaaag tttgtattta tagttagaca
    10081 ttacattaat aattatatga ttattgtttc gaaatcaatt tagagagcat ttcatagaca
    10141 tatctgtgtt cactcattcc ttcgagcagc ttcagatgtt ttgaaattgt ttgaatgtca
    10201 cctcgtttgg caggtcctgt ctgaacaata gaaggatgtt cgtataaaaa agcttttctg
    10261 acagtctcaa ttgccaatgg cataagtagt tcctgatttg gttttgaaag atcgaatgct
    10321 aatccaatca tgtaattgac aaaattactc acgtatacag cggctaaatg cattttaagt
    10381 ctttcgtcag atgtagatat catatattca gaacccaaag acaacacaat atctttgagg
    10441 atcttttctc cctcgggcga gtaggactca ataagaaatg gtactaattt aaaatttaca
    10501 ggtttaattt tacttaaggt cattaaagga tataaaacac ctgtaaatgg ataacattcg
    10561 agtgcttcga tagaagttgc accgctcgta tgtatcagga gtggtatttt tttgtcttta
    10621 aaagtaattg tggcaaattc ttttgccaaa gtttgtatta cgtcatcaga tactgcaaga
    10681 attattatgt cagagtcttt caaagaatgg aagtcattag taaaacatgt tgcctcttca
    10741 actttatgtg gattattttc ttctttgtta aggatgcgta ccaacttttt cccggatgtc
    10801 acgtttcttc cacaaataaa aggaatagag tgaccagcgg ctttcaattc aattgaaagc
    10861 cgataggcca cattacccgt tccccatatt gaaattctgt acataaatga attataagca
    10921 agattaatca aattttgcaa atgtacagat tttataaaac aattacgatt ccggcagata
    10981 ttcttccttt actgcaagaa actggttaag atcacatggt gcaaaatcag caatataaga
    11041 gtatctttcg tcattccagg ctttagactt atttgtaaaa ataatggcag agtctctgaa
    11101 tcttctgtct gactgggcat caattataag aagataagac atgataatat gggatgccat
    11161 ttccacaagt cttcttgcgt gaaaatcaat aaattctgta tcaccacctg aaacaattgc
    11221 aacggcctgc tgatattgct ctgtcatagc aacaagtatc tttttcaatt gtgtgaattc
    11281 tgactttatt tcaatctttt catactcttt aatctgattg agataagctc cgtttgtgac
    11341 atatctttgt gcagccacta cctgcaattg agatgtccct tcgtaaattg ttgtaatacg
    11401 agcatctctg tatattcgtt cgataggata atctttcatg aagcctgaac caccatgaac
    11461 ctgaagtgca tcatatgcat tttggttaga atattcagac gccatcattt tgaccatagg
    11521 cgtgaacatg tctgcaagtc tctgatattg tttggcctca tttttctctt caagagttag
    11581 ttttctggtc tcagcaatca tgttatatgc tttgtacaca tcaacaaaac gggttgtctc
    11641 gtagagaaga gctctagaag cttgaagtct tgccttaatt attgaaagca tttcataaac
    11701 tgctggaaat tgaataatag gcttaccaaa ctgtcttctc tcttttgcaa atttgagagc
    11761 ttctctatag gcagcttccg agatgccaac tgcttgtgct gccacgccaa gtcttgctcc
    11821 attcatcaag gacatgacat atttaataag gcccatacgt ctttcgccca ctagtttggc
    11881 tggagcatta ttgaaaacaa gttcacaagt tggtgaccct ttgatgccaa gtttattctc
    11941 aattcttcga actgtgacgc ctccccattt ctgatcgtga acaaaataag aaagacctcg
    12001 accgtctgta gtattctcct cagaacgtgc aagaaccagc ttgatctgtg catcaccatt
    12061 agtaataaac cttttaacac cgttcaattt ccacataccg cttttctcat ccaaagttgc
    12121 cttaagcatt acagcttgca agtcgcttcc tgcatcaggt tctgtaaggt ccattgagca
    12181 tgtctctcct ttgtttattc tgtgaaggaa ttcatcccta atctcttgat tggcaaattc
    12241 gtatatggta tcagcacaat cttgaaggcc ccaaatattt gaaaatcctg catcagccct
    12301 tccaacaatc tcagcagaca ttatataggg aaccattgaa aagttaagcc ctccgtattc
    12361 gcgaggaaga ttcattccat aaagaccggc ttgtgtcaac acttcatgat tctgttgcgt
    12421 tcctggtgca taggttacat gcccattttc atggtgaggc ccaactttat ctacttcttc
    12481 tgcattttgg gctatgacat ctccgcatat ttctcccaga atatccatga ctctgtcata
    12541 agagtctaaa gcatcctcaa atgaacaggg agcataatca aacttgccaa aatctttaaa
    12601 gtcctgttct ctaagctgta ttattttttt cattagagga tgtgagaact ggaactgaat
    12661 atctttgtta tctctgtaaa aattagccat gttatttgat gtttttcttg taagatttta
    12721 taatctttgg taatacttca ttcaggtcgc caataatggc atagtcagca atattgttaa
    12781 ttggagcatt ggggtcattg ttaattgaaa taatcatggc agactgatcc attcctgcta
    12841 catgctgaat ttgaccggaa attccaactg caatataaag tttgggcctt actgtgacgc
    12901 ctgtttgtcc aacctgtctt gcatgatctg caaatcctgc atctacagct gctctggacg
    12961 ctccaacttc accaccgagc agatgggcca attcaaacaa ttgattgaaa ttttcttttg
    13021 aacctacgcc aaagcctcct gaaacaatta ttgatgcacc tttaatatct atttttttct
    13081 cttgtatgtc tcttctgatt atttcaacta caaaatcaga atcatttaat attaaattga
    13141 catccaattg atttattact ccat
//
LOCUS       DAOTQL010000036        12768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4567_length_12768_cov_4.767561, whole genome shotgun sequence.
ACCESSION   DAOTQL010000036 DAOTQL010000000
VERSION     DAOTQL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_12768_cov_4.767561"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="P5293_03525"
     CDS             complement(<1..362)
                     /locus_tag="P5293_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRR49003.1"
                     /translation="MNNSINNFPLPANEPVLKYLNGSPERIALEKELERQSSTVVEIP
                     LIIDGKEVFTGNTAKVVMPHNHSHTLAVYHKAGEKEVKMAIDAAMKAHVLWSEVNWAT
                     RASIMLKIAELLSTKYRAT"
     gene            478..1356
                     /locus_tag="P5293_03530"
     CDS             478..1356
                     /locus_tag="P5293_03530"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRR49004.1"
                     /translation="MKKSIFFLIAVLFSVAASAQQITKSFVADDFTGITASSVFDIEL
                     IKSPVESVVVTADREVMNYVNIKVVMGQLNLKIETDKMPRILKKNLKSVKVKINMKNE
                     LSWLSLSGACKLTSSSNFTPQNFKGQISGAASICGLEITSESARISISGASSVSMRGK
                     INIADYELSGASNAVMNQEISTLSVKCSGASKMDFKGSIASLDLKLSGSSEVKLKGSG
                     EEIRTQISGASKLIAVEFAANEAYITISGVSKAVVNVSKTLEAEISGSSHLEYKGSPV
                     INNVSVNSISNFKKIY"
     gene            1400..2488
                     /locus_tag="P5293_03535"
     CDS             1400..2488
                     /locus_tag="P5293_03535"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRR49005.1"
                     /translation="MDFQKLHRNAFVLDSHCDSPLKLYEGVDMGKRNDQGHFDFIRMK
                     EGGVDAIFFAIYTSNKLDPDAATRKALQLIARSYDAIEQNEDKVAAALSVEDAFELKK
                     RGLGAIFFGMENGLPIQKDLSLLRLFYDMGVRYMTLTHAGNNDICDSCGSPEKKWHGL
                     SPFGKKVVKEMNRLGMIIDVSHISDESFYDVLKYSKKPVVATHSCCRAIADLPRNMSD
                     QMIKDLAAHGGVIQINFYPPFLNKDYADKFWPLCDAFEVAETKFKKNPKKYEAEYKKA
                     LSDMMALKRPSYTEVVDHIDHVVKLVGVDSVGIGSDFDGIEVTPEGLEGVDKLPVITK
                     ELVKRGYTEENIYKILGGNFLRIMDLNF"
     gene            2493..3251
                     /locus_tag="P5293_03540"
     CDS             2493..3251
                     /locus_tag="P5293_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR49006.1"
                     /translation="MHKTILITGATSGIGEAVAEAFAKEKFRLILTGRRVERLNSLTS
                     RLVKKYEVNILPLNFDVREMAQVEKHLLNLPQEWQHIDILINNAGLAVGLSPLNEGVI
                     DDWERMIDTNIKGLLYVSKIVSNLMIEHGGGHIVNIGSIAGKSVYPNGNVYCATKYAV
                     DALSKGMMMDFIQKNIKVSQVCPGAVETEFSKVRFKGDEKRAAAVYEGFEPLNASDVA
                     DLILYIVKAPAHVNISDVVILPAAQASPTIFCRK"
     gene            complement(3232..4359)
                     /locus_tag="P5293_03545"
     CDS             complement(3232..4359)
                     /locus_tag="P5293_03545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRR49007.1"
                     /translation="MRNIVVFLLIISSMLTSCTSNRKEPGVLTVNKIDSLFQSLYPSN
                     EPGAAVLIMKGDSIVFDKGYGIADLEEMTKIDGNTFFNIASVSKQFAAVAVMMLAEEG
                     KLSLDDNVKKWFPYFKADFFEKIKIKHLLSHTSGIPDARDRSNRNFVLTATDVASYSY
                     MKNLDTLNFEPGSDYEYMNPTFQLLYTIIEKASGMQFDTFMKNKIFLPCGMNETTYFE
                     AGKEIPRMAHGYLLDSTSNNFREFDFGEESFFATKADGGIYTSTREFAKWEIALRDNK
                     LISEQMKNTAHSPKISIKDMPYTFYGYGWFIEEKPGYPVKVFHTGDNGGFQIYAGRYP
                     DNKILYLIFSNRNDRDRELTISKLDKILKEAGWLDTTSDRK"
     gene            complement(4415..5680)
                     /locus_tag="P5293_03550"
     CDS             complement(4415..5680)
                     /locus_tag="P5293_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HRR49008.1"
                     /translation="MLNASICTIGDEILIGQIVDINSAIIAKELNSIGVKVNKIISVQ
                     DDENEIISSLQECYKTSDLIIVTGGLGPTKDDITKKALAKLCNCEQYKYDINQARLIE
                     DICNKRNIALTKLNRDQAFVPECCEVIINRLGTAPGMIFRLNNKNKNVILFSLPGVPY
                     EMEHMLPAVIETVKKEFKIDSIYHRTLLTFGIPESTLASMIENWEDNLPDEIKLAYLP
                     NPHTGVKLRLSIYGGDLDNNVQLVEKEVVKLKQILGNAIYGEGDESLESVLSKYLTYN
                     HKTISVAESCTSGRISSILTSIPGSSVYFKGGIVAYDNSIKMNILNVKEDTLNNFGAV
                     SAKCVEEMAENVRKLMKSDFSIATSGIAGPSGGSTDKPVGTVWIAISGPNFLESQMKV
                     FNGNRNNNIERFSTEALNFLRLRLGLQLK"
     gene            complement(5682..6713)
                     /locus_tag="P5293_03555"
     CDS             complement(5682..6713)
                     /locus_tag="P5293_03555"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRR49009.1"
                     /translation="MYKILRPLLFRFPPEWTHNMTLNLLKFLRYLPFSGTFIRWIFSY
                     NDEKLEKEVFGLKFKNPLGLAAGFDKNGEMYNDLANLGFGFIEIGSLTPVEQIGNPRP
                     RVFRLVEDNAIINRMGINNKGVKNAIKHLKTHKPKIIVGGNISKNASCSNETAAIDYE
                     KSFTQLYDFVDYFVLNISCPNVKGLVKLQDINILSEIIDRLTAIRKFNDEYRPILIKI
                     SPDITFDQLDEIIELILISGIDGIVATNTTTSRESLKTSKDILKKIGDGGLSGEPLFN
                     KTLEIVKYIHRKSGGMFPIIAVGGITTVERAKEMLDAGASLIQIYTGFIYNGPGFVKK
                     ILKHLSNNQ"
     gene            complement(6741..7163)
                     /locus_tag="P5293_03560"
     CDS             complement(6741..7163)
                     /locus_tag="P5293_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRR49010.1"
                     /translation="MKERLQQFLNAEQLSSSKLSDILGIQRSGMSHILSGRNKPSFDF
                     IQKLLLKFPSLNAEWLITGKGNMYKENSDASLYANRAEYQSDEKENEDYKPPEILINE
                     SEKIEAQNNRRLIKIILIYSDGTFSDFRHERIKADNDN"
     gene            7652..8896
                     /locus_tag="P5293_03565"
     CDS             7652..8896
                     /locus_tag="P5293_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRR49011.1"
                     /translation="MYKFARKNILIPEIKISDYTYILPDSKIAKYPLQSREDSKILIF
                     ENGLISDSKFSNLDAILPGKSIMVLNNTKVVPARLIFQKKSGANIEILCLEPYFPAEY
                     NQSFAQNKFCTWKCLVGNRKRWKEGYVYFYSNGNEELEKYDLKAKLVGKENDKTLVEF
                     SWNNGIIFSDLMDKCGKVPIPPYLNREAEDLDTDRYQTLYAQIRGSIAAPTAGLHFSE
                     DVLKKISEKGIEIENICLHVGAGTFIPVKSEFISDHNMHSELFSITKNTIKNLINKGD
                     KKIIAVGTTSTRTLESLYYLGVNCIENKSIEPVKQWDPYNKDYKYSTNEALEALLEWM
                     NENKRDYIETKTGIIIVPGFQFRLVDVLITNFHQPNSTLLLLIATFVGDYWREIYSYA
                     LANNFRFLSYGDSSILFRSVDQ"
     gene            complement(8877..10640)
                     /locus_tag="P5293_03570"
     CDS             complement(8877..10640)
                     /locus_tag="P5293_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HRR49012.1"
                     /translation="MMFNTCSPYIKLLFDKGIDWYPYLDQKLIERAEKEDKLIFQHIG
                     YISNMKVREMAERLFSDPRVINILNDNFICIVEDKESSPESYLIALDLLFLNQDFSFG
                     PMNLFVMPDRKPIICFSDCNPEYFLEVAGSIVDAKKNKKYLLKQLSEELSKSALNTGI
                     ITEKVSPSTISSKILDTYIHQWYNGIAENDFIYQITPFTPNPSTLLTIVEYLKFYKDK
                     TISDKIESMLDHLQFSSVFDPIGGGFFKQAKDYSCKDPSFEKTLEDNAHFINLYANAY
                     NYFKKESYKITSWMTYKFIQDELATQSGGYKSSTTITSSLDEVNYYKYSINELMIMFP
                     ERYIDVAIALNLDPLADKHLKQIPARTSRTYSILSEDEIEKLKIRRTEHIGYFADRRE
                     ITSYMSQLAESLASSSLMLNDRSLYETALNIVRYIITNNSHSDGRLYRYCCCNKSNIP
                     GYLSDYADFIAALIELYKSDNCKNQRLFDYAKKYIDFVITHFYKPENGMFNKSECSIE
                     PNTIPFKRESNIDFLKPSANSIMAGNLLSFYEITGDKEYFKIAEQQIMNIAPNLHSSG
                     PMLSSWAHKVLKYLNIIDQPT"
     gene            10777..11925
                     /locus_tag="P5293_03575"
     CDS             10777..11925
                     /locus_tag="P5293_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRR49013.1"
                     /translation="MENEKFSEIRPFNDFETREAMLRISKHPLLSPIIQYLFPDKNLY
                     EMREHIASLKTVDEFQAKVMSNVIQKIISDTSRNLTYSDVERFKDNKKHLLISNHRDI
                     LLDSAIIQLIFFVNGIQTSEMAVGDNLITDVFIEDIARSNKMIKVERNKNPRDFYNSS
                     ILLSHYIRETITSGRSSVWIAQRNGRTKDGKDMTEQGLLKMFDMSAPSGDFVEDFNDL
                     SIVPVSISYEYEPCDLLKTKELYISRRRKYEKAPGEDLNSILTGIMQFKGNIHVNFCD
                     TITREELTWCSNMEKNDRYKNLAEIIDRKIISSYKLFKNNYIAYDLLNHSNKYSSYYS
                     ESDRQSFLSYINYKLSGIDEGQKEMEEIFLTIYSGPVFNNERIEVTDI"
     gene            12078..12728
                     /locus_tag="P5293_03580"
     CDS             12078..12728
                     /locus_tag="P5293_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRR49014.1"
                     /translation="MNKLINLEEAMSKIKSGMTVMVGGFLTKGGPNLFMETLADTDVS
                     NLTLICNDSAYADRGLGKLIANKKVKKLIVSYIGSNSAAIDLMNSGEMEVEFSPQGTL
                     IERIRAYGAGLGGVLTPTGLGTIVANNKEIINIDGKEYLLEKPLKADVAVVGASISDT
                     IGNLFYKGTKRNFNPVIAMAADIVIVEPEKIVECGDIEPEHVHTPAILIDYIISKN"
BASE COUNT         4219 a         1991 c         2195 g         4363 t
ORIGIN      
        1 gttgccctgt attttgtgga cagcagttct gctattttta acattataga tgctctggtt
       61 gcccagttta cctcactcca caaaacatga gccttcatgg cggcatctat tgccatcttt
      121 acctcttttt ctcctgcttt atgatatact gcaagagtat gtgagtgatt atgaggcatg
      181 acaacttttg cagtgttccc tgtaaatacc tctttcccgt caataataag aggaatttca
      241 acaacagttg aactttgtct ttcaagttct ttctcaagtg caatcctttc cggactgcca
      301 tttaaatatt tcagtaccgg ttcatttgct ggtagaggaa aattgttaat ggaattattc
      361 atatctttat ttaaaaatta attcgaatgc aacaaattta aataattttc gtctaattaa
      421 tggagatttg taagagaaaa gtttatttat tgatatatat atatttaatt attttttatg
      481 aaaaagtcaa tatttttttt aatagcagta ctcttttcag ttgctgcttc tgcccaacag
      541 atcacaaaaa gttttgtggc tgatgatttt actggaataa cagcctcaag tgtatttgac
      601 attgaactta taaaatcacc tgtagaatct gtagtagtta ctgctgaccg agaagttatg
      661 aattatgtta acataaaggt agtaatggga cagcttaatc ttaaaattga aactgataag
      721 atgccgagaa ttcttaaaaa aaatctcaaa tccgtgaaag ttaaaattaa tatgaaaaat
      781 gagctttcat ggctttctct ttccggagct tgtaaattaa cgtcctcgtc taattttact
      841 ccacagaatt tcaagggaca gatcagtggt gctgcttcta tttgtggtct tgaaatcacg
      901 agcgaaagtg cacgtatttc aataagtggt gcttcttcag tatccatgag aggcaaaata
      961 aatattgcag attacgaatt gtcgggagcc agtaatgcag ttatgaatca agaaatttcg
     1021 acactttcag taaagtgcag tggagcatcc aagatggact ttaaaggtag tattgccagt
     1081 ttggatctga aactttcagg ttcttcagaa gtgaaattga aaggtagtgg agaggagata
     1141 agaactcaga tttcaggagc ttcaaaatta attgccgttg aatttgctgc caatgaggct
     1201 tatattacca taagtggtgt ttctaaggct gtagtcaatg tctcaaaaac acttgaggcg
     1261 gagatctcgg gaagttcaca tcttgaatac aagggttctc cggtcataaa taatgtatcg
     1321 gtcaattcca tttcaaactt caaaaagata tactaatctt cgttttttat tacctttata
     1381 aaaaataaat aaaggtaata tggacttcca gaaactgcat agaaatgctt ttgtccttga
     1441 ttctcattgt gattctccat taaaactcta cgagggagtt gatatgggta aaagaaacga
     1501 ccagggacat tttgatttta taaggatgaa agagggtgga gtagatgcga ttttttttgc
     1561 tatttacaca tccaataagc ttgatcctga tgctgcaaca agaaaggcac tgcaacttat
     1621 tgcaagaagc tatgatgcta ttgagcaaaa tgaagacaag gttgctgcag cacttagcgt
     1681 cgaagatgct tttgaattga aaaaaagggg attgggagct attttctttg gtatggaaaa
     1741 cggacttcct attcaaaagg atctttcctt actaagactt ttttatgata tgggagttcg
     1801 ttatatgact ctcactcacg caggcaacaa tgatatttgt gattcatgtg gttctcccga
     1861 gaagaaatgg catggtctaa gtccctttgg taaaaaagtt gtgaaagaga tgaacaggtt
     1921 ggggatgatt attgatgttt ctcatatttc tgacgaatct ttctatgacg ttttaaaata
     1981 ttcaaaaaaa cctgttgtag caactcattc atgttgcaga gcaatagctg atcttccaag
     2041 aaatatgagt gatcagatga tcaaagattt agcagcacac ggcggagtta ttcaaattaa
     2101 tttttatcct ccttttttga ataaggatta tgcagataaa ttttggcctc tatgtgatgc
     2161 ttttgaagta gcagagacaa aattcaaaaa aaatcccaaa aagtatgaag cagaatataa
     2221 aaaggctttg tctgatatga tggcattaaa aagaccttca tatacagagg tggttgatca
     2281 cattgaccat gtagttaaac ttgtaggtgt tgacagtgtt ggtattgggt ctgattttga
     2341 tggcattgaa gttacacccg aaggtcttga aggggttgat aaacttcctg ttattacaaa
     2401 ggaacttgtg aaaagaggtt atactgaaga gaatatttac aaaatactag gaggtaattt
     2461 tctaagaata atggatctaa atttttaaaa tcatgcataa aacaatattg attacagggg
     2521 caacatctgg aataggagag gctgttgctg aagcgtttgc caaagaaaag ttcagattaa
     2581 ttcttacagg cagaagagtt gaaaggttaa attccctaac aagtcgtctg gttaaaaaat
     2641 atgaagtaaa tatactgcca ctgaattttg atgttagaga aatggctcag gttgaaaagc
     2701 atcttctcaa tttgccacaa gagtggcaac atattgacat attgattaac aatgctggtc
     2761 tagctgtagg tttaagccct ttgaacgagg gtgtcattga tgattgggaa aggatgattg
     2821 atacaaatat caaaggactt ctctatgtta gcaagatagt ttcaaattta atgattgaac
     2881 atggtggtgg gcacattgtg aacataggct caattgcagg taagagtgtt tatccaaacg
     2941 gtaatgttta ctgtgcgacc aaatatgcag ttgatgctct atcaaaaggc atgatgatgg
     3001 attttatcca gaagaacata aaagtatctc aggtttgtcc tggtgcagtt gaaacagaat
     3061 tttcaaaagt tcgtttcaag ggagacgaaa agcgagcagc agcagtatat gaaggatttg
     3121 aaccattgaa tgcttcagat gttgcagatt tgattttata tatcgttaag gctccagcgc
     3181 atgtaaatat tagtgatgta gtaatattac ctgcagcaca ggcatctccc actattttct
     3241 gtcggaagta gtatcaagcc atccggcttc ttttaatatt ttatccagtt ttgaaatagt
     3301 taattctctg tctctgtcat ttctatttga aaaaatgaga tagagaattt tattgtcagg
     3361 atatcttcct gcatatatct gaaagccacc attatcacca gtatgaaaca ctttaacggg
     3421 ataacctggt ttttcttcta taaaccatcc atatccgtag aacgtatatg gcatatcttt
     3481 aatggatatt ttaggagaat gagcggtgtt tttcatctgc tcactgataa gtttgttgtc
     3541 tcttaatgca atttcccact ttgcaaattc tcttgtcgaa gtataaatgc ctccatcagc
     3601 cttagtcgca aaaaaggact cttctccaaa atcaaattct ctaaagttgt ttgatgttga
     3661 atctaacaaa tatccatgtg ccattcttgg aatctctttt cctgcctcaa aatatgtggt
     3721 ttcattcata ccacacggta gaaagatttt atttttcata aaagtatcga actgcatacc
     3781 tgaagccttc tcaatgattg tgtataataa ttgaaaagtt ggattcatat attcgtaatc
     3841 tgatccgggc tcaaaattga gagtatcgag attcttcata tatgaatagg aagcaacatc
     3901 tgtagcagtc agaacaaaat tcctattact ccgatctctc gcatccggaa ttcctgatgt
     3961 gtgggatagc aagtgtttta tctttatttt ttcgaaaaaa tcagctttaa aataagggaa
     4021 ccactttttg acattatcat ctaatgaaag tttcccctcc tctgcaagca tcattactgc
     4081 tactgccgca aattgcttag aaacagaagc tatattaaaa aaagtatttc catcaatttt
     4141 tgtcatttcc tccaaatctg caattccata acctttgtca aaaacaatag aatccccttt
     4201 cataattaaa acagctgccc ctggctcatt agaaggatag agagattgaa aaagagagtc
     4261 aatcttatta acagtcaaaa cgcctggctc ttttctattt gaagtacatg aagtcaacat
     4321 acttgatatt attaacaaaa acacaactat atttctcata aacaataaaa tataatattg
     4381 tatcacaatt tagttacatt atgtaacgta taaattactt tagctgtaaa ccaagtctta
     4441 gccttaaaaa gtttaatgct tccgtagaga atctttcgat attgttattc ctgtttccat
     4501 taaaaacttt catttggctt tcgagaaaat tgggaccgga aattgctatc catacagtac
     4561 cgactggttt atctgttgaa cctcctgaag gacctgcgat tcctgatgta gcaattgaaa
     4621 agtcactttt cattaactta cgcacatttt cagccatttc ttcaacgcat ttagcactta
     4681 ctgctccaaa attattaaga gtatcttctt ttacattgag gatattcatt ttgatgctgt
     4741 tgtcatacgc tacaattcca cccttaaaat aaacagatga accaggtata gatgtaagga
     4801 ttgacgaaat tcttcctgaa gtacaagatt ctgctacaga aatagtttta tggttgtatg
     4861 ttagatattt agataaaact gactcaaggg actcatctcc ttctccataa atagcatttc
     4921 caagaatttg tttgagcttg actacttctt tttcaactaa ttgaacatta ttatctaaat
     4981 ctccaccata aatagataat cgcaatttga ctccagtatg tggatttgga agatatgcca
     5041 gtttgatttc atcaggaagg ttgtcctccc aattttcaat catagaagct agagtagact
     5101 caggaattcc aaaagtcaaa agtgttctgt ggtatatgga atctattttg aattcttttt
     5161 ttacagtttc aattactgcc ggtaacatat gttccatctc atatggcaca ccgggcaaag
     5221 agaatagaat cacattttta tttttattgt ttagtctgaa tatcatacct ggcgctgttc
     5281 ccaacctgtt gattattact tcgcaacatt caggaacgaa agcctgatct ctattaagtt
     5341 tagtaagagc aatatttctt ttattgcaga tatcttcaat taaccttgct tgattaatat
     5401 catatttgta ttgctcacag ttgcaaagtt ttgccagagc tttcttagtt atgtcgtctt
     5461 tagtagggcc cagacctcct gtaacaatta ttagatcact tgttttataa cattcttgta
     5521 aggatgaaat aatctcattt tcatcatctt ggactgaaat tattttattt actttaactc
     5581 caatactgtt taattcctta gctattatcg cagaatttat atccacaatc tgacctatta
     5641 gtatttcgtc tccgattgtg cagattgatg cattcaacat tttactggtt gttagataag
     5701 tgtttaagta ttttttttac aaaaccagga ccattatata tgaatccggt atatatttga
     5761 attagagaag ctccggcatc tagcatctct ttggctcttt ctaccgttgt aatgcctcca
     5821 acagcaatta ttggaaacat tccaccactt tttctatgaa tatatttaac tatttcaaga
     5881 gttttattga acaacggttc accactaaga ccgccgtctc caatcttttt tagtatatcc
     5941 ttgctagtct tgagagactc tcttgaagtt gttgtattag ttgctacaat accgtctatt
     6001 ccggaaataa gtatcaattc tattatctcg tctaattgat cgaatgtaat atctggagat
     6061 atttttatca atattggacg atattcgtca ttaaatttac gaattgcagt aagtctgtca
     6121 atgatttctg ataaaatgtt aatatcctga agtttaacca atcctttaac atttggacat
     6181 gaaatgttta aaacaaaata atctacaaaa tcataaagct gagtaaatga tttttcataa
     6241 tcaatagccg ccgtttcatt tgaacatgag gcatttttac tgatattgcc accaacaata
     6301 attttaggtt tatgtgtttt tagatgtttt atagcatttt ttacaccttt attgtttatt
     6361 cccattcgat ttataatggc attatcttca accagtctaa atactcttgg tcgtggattg
     6421 ccaatttgtt ctactggagt aagagaccca atctcaataa aaccaaatcc cagattagca
     6481 agatcgttgt acatctctcc attcttatca aatccggcag ctagtcccag tggattttta
     6541 aatttaagac caaatacctc tttctcgagt ttttcgtcat tatatgagaa tatccacctt
     6601 ataaaagttc ctgaaaaggg tagatatctt aagaacttca gtagattcaa ggtcatgttg
     6661 tgagtccatt caggaggaaa tcggaaaaga agaggtctaa gaattttata cattataaat
     6721 cctttttgca aagttagaaa ttaattgtca ttatcagctt taatcctctc atgtctgaaa
     6781 tcagagaatg taccatcaga gtagatcaag attatcttta tgagacggcg attattctga
     6841 gcttctatct tttcagactc atttatcaaa atctcaggag gtttataatc ctcgttttct
     6901 ttttcatcac tttgatattc agctcgatta gcatataaac ttgcgtcaga gttttcttta
     6961 tacatatttc cttttcctgt aataagccat tctgcattaa gagaaggaaa tttcaatagt
     7021 aacttttgaa taaaatcaaa gcttggttta tttcttccag atagaatgtg tgacatccca
     7081 gaacgttgta ttccaaggat atcagataac ttggaagaag ataattgttc cgcatttagg
     7141 aattgctgta aacgttcttt catggctaag acttattttt acaaatgtaa tcattatatt
     7201 aattacatgt gtaactactc cttattatac aaatgtaaac agaataagaa tgaaattttt
     7261 aaatatattt actatgattt agtaatcaaa catttatcat ttattgacta atcttaacaa
     7321 ctggtttata taagacttat aaatacctgg taaataataa tttataatag taatatatca
     7381 tttttatcaa taatttatgg taaaatatta caaaactaat aaattaacct atgtttttac
     7441 tttatataaa ttaagtaaat atctggttat taatataaaa tataataaaa attatttgta
     7501 aatttacata tgtaaacatc acaacttagg gaacatatat aacttgcaat gactatttat
     7561 ttaacatgtg taaacagtta gtttggatta attttattac atgtgtaaca taaggtgagg
     7621 aataatttct atatttcaaa catgtggaca tttgtataaa tttgcaagaa aaaatatttt
     7681 gatacctgaa attaaaatta gtgattacac ttacattttg cctgatagca aaattgcaaa
     7741 ataccctctt caaagtcgtg aagactccaa gattttaata tttgaaaatg gactgatatc
     7801 tgattcgaaa ttttcaaatc tcgatgcgat attgcctgga aagtcgatta tggtgttgaa
     7861 taacacaaag gttgttcctg caagattaat ttttcaaaaa aaaagcggag ctaatattga
     7921 aattctttgt ctagaacctt attttcctgc ggaatataat caatcctttg ctcagaacaa
     7981 gttttgtact tggaaatgtt tggtaggtaa cagaaagaga tggaaggaag gctatgttta
     8041 tttttattca aatggaaatg aagagttaga aaaatatgat ctaaaggcaa aactagtcgg
     8101 taaggaaaac gataaaactt tggttgagtt ttcctggaat aatgggatta tcttttcaga
     8161 tttgatggac aaatgtggta aggtgcctat tcctccatat ctaaatcgcg aagcagaaga
     8221 tttagacact gatcgatatc agactcttta cgcacaaata cgcggttcaa tagcggcacc
     8281 aacagcaggc cttcattttt ccgaagatgt tttaaaaaaa attagtgaaa aaggaataga
     8341 aatagaaaac atctgtttac atgttggtgc aggaacattt attcctgtta aatctgaatt
     8401 tatcagtgat cataatatgc attcggaact gttttcaatt acaaaaaata ctattaaaaa
     8461 tctgatcaat aaaggtgata aaaagataat tgctgttggg acaacttcaa caagaacact
     8521 tgaatcactc tattatctag gagttaattg tattgaaaat aaaagtattg aacctgtaaa
     8581 acaatgggac ccgtataaca aagattataa atattctact aatgaagcat tggaagcttt
     8641 gcttgaatgg atgaatgaaa ataaaagaga ttatattgaa actaaaaccg gtataataat
     8701 tgtccctggt tttcaattca ggctagttga tgttttgatt acaaattttc atcagcccaa
     8761 tagcactctt ttactattaa ttgccacttt tgtaggtgat tattggagag aaatttatag
     8821 ttatgcattg gcaaataatt tcagattttt gagttatggt gacagctcaa tactgttcag
     8881 gtcggttgat caataatatt taagtatttc agcactttgt gagcccaact tgagagcatt
     8941 gggcctgaag aatgaagatt gggagctatg ttcattattt gttgttctgc gattttaaaa
     9001 tactctttat ctcctgtaat ttcataaaag gatagaagat ttccggccat aatagaatta
     9061 gcagaaggtt tcaaaaaatc tatatttgac tctcttttaa atggaatagt attcggctca
     9121 attgaacact ctgatttatt gaacattccg ttttccggtt tataaaaatg ggtaataaca
     9181 aagtcaatat atttttttgc ataatcaaaa agcctttgat ttttacaatt atctgattta
     9241 taaagttcta taagggcagc tataaaatct gcataatctg acagataacc gggaatattg
     9301 cttttgttac agcaacaata tcgatatagt ctaccgtcag aatgactgtt atttgttata
     9361 atatatctaa cgatatttaa tgccgtttca tataatgatc tatcattaag cattagtgag
     9421 gaagatgcaa gagattcagc taattgcgac atgtaagatg ttatttctct tctgtcggca
     9481 aagtaaccta tatgttcagt tctcctaatt ttaagttttt caatttcatc ttcactgaga
     9541 attgaatatg ttctcgaagt tctggcagga atttgtttta aatgtttatc tgcaagaggg
     9601 tcaaggttga gagcaattgc tacatcaata tatctttcgg gaaacattat cattaactca
     9661 ttaattgaat atttataata attgacttcg tctaaagagc ttgtaattgt tgtactactt
     9721 ttgtaaccac ctgattgtgt agctaattca tcttggatga atttataagt catccaagat
     9781 gttattttat atgattcctt tttgaaataa ttatatgcat ttgcatataa attaataaaa
     9841 tgagcattgt cttctaaagt tttttcaaaa gaaggatctt tacagctata atcttttgct
     9901 tgtttgaaaa agccacctcc gataggatca aaaacagaag aaaattgcaa atgatcaagc
     9961 atcgattcaa ttttgtcaga tatggtttta tctttataaa actttaaata ttcgacaatt
    10021 gtcaacagcg tacttggatt tggagtaaaa ggagtgatct gatatataaa atcattctca
    10081 gctattccat tataccattg atgtatgtat gtatctaaga ttttcgaact aatagtggaa
    10141 ggagacactt tttctgttat tattcctgtg tttaatgcgc tttttgataa ttcttctgaa
    10201 agttgtttaa gaagatactt tttatttttt tttgcatcaa caatacttcc tgcaacttcc
    10261 agaaaatatt caggattaca atcagaaaaa caaataattg gttttctgtc cggcataaca
    10321 aataagttca tagggccaaa cgaaaaatcc tgattaagaa acagtaggtc tagtgctata
    10381 agatatgatt cagggctact ttctttatct tcaactatac atataaaatt atcattcaaa
    10441 atattaatta ctcttggatc agaaaagaga cgttctgcca tttctcttac tttcatattg
    10501 cttatataac caatatgttg aaaaattagc ttatcctctt tttcggctct ttcaattaat
    10561 ttctggtcca aatacggata ccaatcaatt cctttatcaa acagcaattt tatgtatgga
    10621 gaacatgtgt taaacatcat gtatattaat ttgtcaataa ggaaatgtta atgactgtac
    10681 aacaattcga tggttatttt gttcattaga cattagagaa agaatatttt cttgaatata
    10741 atcttgatgt tggaagaaaa ataattattt ttgcatatgg aaaatgaaaa atttagcgaa
    10801 ataagaccat ttaatgactt tgaaacaaga gaggcaatgt tgcgtatttc taaacatcct
    10861 ttgctctctc ccattattca atatcttttt ccagataaaa atctgtatga aatgagagag
    10921 catatagcat ctctcaagac agtggatgaa ttccaggcta aagtcatgtc caatgtcata
    10981 cagaagataa tttctgatac ctctagaaat cttacatatt ctgatgttga acggtttaag
    11041 gacaacaaga agcatttgct tatttccaat cacagggata ttttgcttga ttcagccatt
    11101 attcagctga tttttttcgt aaatggtatt caaacttctg agatggcagt cggagataat
    11161 cttattactg atgtttttat tgaagatatt gcacgttcta acaaaatgat aaaggttgaa
    11221 aggaataaaa atcctcgtga tttttataac tcttcaatac tactttctca ttatattcgt
    11281 gagacaataa caagtggccg tagttctgtg tggattgctc aaagaaatgg gcgtaccaaa
    11341 gatggtaagg acatgacaga acagggactt ctcaaaatgt tcgacatgag tgctccttcg
    11401 ggagattttg ttgaggattt taatgatttg tcaattgtac cagtttctat atcatatgaa
    11461 tatgaacctt gtgatctctt gaaaactaag gaactctata tttcaagaag aagaaagtat
    11521 gaaaaagctc ctggagaaga tctgaacagc attcttaccg gaataatgca gtttaaaggt
    11581 aatattcatg tgaatttttg tgacacaatt acccgtgaag agttaacatg gtgttcaaat
    11641 atggagaaaa atgatcgtta taaaaatctt gcagagatta tagacagaaa gatcatcagt
    11701 tcctacaagc tgtttaaaaa taattacatt gcttatgatt tgcttaatca tagcaataaa
    11761 tactcttctt attattccga atctgacagg cagtcttttt taagttatat caattataaa
    11821 ctctcaggaa ttgatgaggg acaaaaagag atggaggaaa tcttcctcac aatatactcc
    11881 gggcctgttt ttaacaatga aaggatagaa gtaacggata tatagctacc tgctattatg
    11941 taatagtagg tgctctatga aatgtaacta cgatgttgtt aagaacaact tgttagttac
    12001 attctctatt ggtgaactag ttagcctgat cattacaaaa aataatatac tgtataaatt
    12061 aataaaatct taaaaaaatg aataagttaa ttaatcttga agaggccatg tcaaaaataa
    12121 aaagcggcat gactgttatg gttggaggat ttcttaccaa aggtggtcca aatttattca
    12181 tggagactct tgccgacaca gatgtaagca atttgacttt aatttgcaat gactcagcct
    12241 atgcagacag aggacttggt aaattgattg caaataaaaa ggtaaaaaaa ttgatagtat
    12301 cctatatagg atcaaattct gctgcaatcg atttaatgaa ttccggagag atggaggttg
    12361 aattttctcc acagggaacc cttattgaaa ggattcgtgc ttatggtgcc gggttgggag
    12421 gtgtattgac tccaacaggt ttgggtacta ttgtagcaaa taataaagaa ataattaaca
    12481 tagacggcaa ggaatatctt ttagaaaagc cactcaaggc tgatgttgca gttgttgggg
    12541 cttccatatc tgatactatt ggaaatctat tttataaagg aacaaaaaga aactttaatc
    12601 ctgtaattgc aatggctgca gatatagtta ttgttgagcc tgagaaaatt gtagaatgtg
    12661 gcgatattga gccagaacat gtgcataccc ctgctatatt gatagattac attatttcaa
    12721 aaaattaata aaatatatta tgtcagaaaa atcaatgatt cgcatgag
//
LOCUS       DAOTQL010000037        12598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4629_length_12598_cov_5.734354, whole genome shotgun sequence.
ACCESSION   DAOTQL010000037 DAOTQL010000000
VERSION     DAOTQL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_12598_cov_5.734354"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="P5293_03585"
     CDS             complement(<1..242)
                     /locus_tag="P5293_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR49015.1"
                     /translation="MQITSEFLHEQLKRFFGFDSFKWQQEDIINHLINGGDAFVLMPT
                     GGGKSLCYQLPALLMDGTAVIISPLIALMKNQVDAI"
     gene            338..1309
                     /locus_tag="P5293_03590"
     CDS             338..1309
                     /locus_tag="P5293_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRR49016.1"
                     /translation="MTISKEELAQIAQEVINKEATSIARLAGFIDSQFIDILHLIFNS
                     KGRLIVTGIGKSAIIANKVVATLNSTGTPAIFMHAADAIHGDLGIIQKDDIVLCLSKS
                     GNTAEIKVLVPWIKKMGNKIIGMVSDTNSFLAKNSDYLIRATVDSEACPNNLAPTSST
                     TAQLVMGDALAVCLLKMRGFSQEDFAKVHPGGSLGKKLYTRVSDLIDRSVKPMVREDD
                     SIQNTILNISANRLGATVVLHENGEIAGIITDGDVRRMVEKGIDIQTLRASDIMSPNP
                     KKIDAEELAVKAFNMMEQNKITSLVVIEKGKYIGLVHIHDILREGIV"
     gene            1306..2346
                     /locus_tag="P5293_03595"
     CDS             1306..2346
                     /locus_tag="P5293_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRR49017.1"
                     /translation="MTEFNPNDIGVANGNFFGLPFKPEQSEIVLISVPWDVTTSYRSG
                     TSYGPQAIMNASLQVDLFDNHVKRAWETKIGTIPEDYSIRQDNKKVRKIAEKVIEHLA
                     KGESLQSVQSEVDTVNTASAALNSNIYRISKDYISKGLTAGIVGGEHSVPFGYFTALS
                     EIYEDFGILQIDAHADLRKAYEGFKYSHASIMYNALNEIPQISQLTQVGIRDYCQEEA
                     DFINRDPRIRSFTDYEIRTNIFEGKVWKRQCEEIISSLPQNVYISFDIDGLSPDNCPN
                     TGTPVPGGLSFMEADFLLYMLALSNKKIIGFDLCEVAPASDNEWDANVGARLLYKLCI
                     YSQYNSKRQLDR"
     gene            2343..3602
                     /gene="nhaA"
                     /locus_tag="P5293_03600"
     CDS             2343..3602
                     /gene="nhaA"
                     /locus_tag="P5293_03600"
                     /inference="COORDINATES: protein motif:HMM:NF007111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRR49018.1"
                     /translation="MSQQTTPITKIKRPSTKSAFRSFLNNQHSGGIALLIFASISIIA
                     ANFEPLNFLTKLWDREIGFSLGSFELKMSLLHWINDGLMALFFLSVGLGIKRELVTGE
                     LSSIKRASLPIIAALGGMIFPALIYSAFNYNTPTQNGWGIPMATDIAFAIGVISLMNK
                     RVPHSLKVFLTALAIVDDLGAIIVIAIFYPTHDINFTMLLWASAIMIILIAMNKKNVQ
                     NAVFYILPGLLLWLFILKSGIHSTIAGVLLAMTIPVKMKEDNLTKNRVPLLKKMESFI
                     TPIVTFIIVPVFALANAGVKFSIDLSGPLQNISKGIFCGLVFGKPIGIFLSSYVACKI
                     GLADLPTNSNWKMLFSVGIIAGIGFTMSIFIDNLAFEDPDIVAAGKRVVLLSSLTAAI
                     IGSLILYASTKKTMETNKPNKQKNENN"
     gene            3589..4323
                     /locus_tag="P5293_03605"
     CDS             3589..4323
                     /locus_tag="P5293_03605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRR49019.1"
                     /translation="MKTIKFISVILIALLVSTSCGKKESTSTNSNYGISQSAIDSASY
                     AVGITLGSMIKQSNFGELNLKEVTKAMKAVIDGDSLKISMIKANEVIQNFLMKRQEAI
                     GAENKTKGEEFLKANATKDSVVTTASGLQYIIINPGSPLKPTETDTVQVHYVGTLIDG
                     TEFDSSYKRGEPARFPLNGVIKGWVEGVQLIGEGGKIKLFIPSELAYGAQQAGPQIGP
                     NSTLIFTIDLLKVSPSKPVAETKAAK"
     gene            4332..4727
                     /locus_tag="P5293_03610"
     CDS             4332..4727
                     /locus_tag="P5293_03610"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRR49020.1"
                     /translation="MAIEITGKLIKKLPLQSGSTPRGEWKKQEFIIETQENYPKKICM
                     SVWGDDGISDIERHSEGDILKLSVNIESREYNQRWYTDVRAWRIEKLSGENTDNSPHY
                     NSQPEHQNSSQISNSENYDSDSEIDDLPF"
     gene            complement(4800..5675)
                     /gene="miaA"
                     /locus_tag="P5293_03615"
     CDS             complement(4800..5675)
                     /gene="miaA"
                     /locus_tag="P5293_03615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRR49021.1"
                     /translation="MIRKRLTIIVGPTAAGKTDYAISLALKFGSPVISCDSRQIYREM
                     KIGTAPPTPEQLSLVKHYFIFSHSIHDDYTAGQYEMEALPLIETLFENHDNLVMVGGS
                     GLYVDAICKGMDDFPKSDPELRMTLMSRLKTEGLYSLRSELRLLDRESYDSIDITNPQ
                     RVIRALEVTLMTGRKFSSFKSYTQKKRNFEIEKIVVDRPREELYERINLRVEKMMEAG
                     LLEEATGLYQFRKRTALKTVGYSELFDYIDGKVSLDEAVWLIKRNTRRYAKRQITWWR
                     REGLIPSESIKRRSR"
     gene            complement(5668..6147)
                     /locus_tag="P5293_03620"
     CDS             complement(5668..6147)
                     /locus_tag="P5293_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49022.1"
                     /translation="MVYRFKASIAGNKIFMREYEIKGNTSLYSLHLFLQNDLGFAPDQ
                     MVVFRGLNGEGKVKSEYGLFDLGDGAMDSISVEKVVNKGEVKLQYVYDIYKDKAIDLE
                     FLSTEEESLKRSYPRLVAERGKNPDQFSDNYDDFEQMIEMGDSDSEDFLGGQEDTDD"
     gene            complement(6172..7197)
                     /locus_tag="P5293_03625"
     CDS             complement(6172..7197)
                     /locus_tag="P5293_03625"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRR49023.1"
                     /translation="MYSKVFIDDFSALGDRLKATLSGSVFDRKFEAALRNSFKGNPLF
                     TLNMQHIALTSICNCFLDKSKLEKWLLDYSDRIEERQGEVLAVMAGNIPLVGFHDFMT
                     ILAAGFRAKIKLSSKDSYLLPYILGVLFEIDDYWSDRVEIIDYLPLCADILIASGSDN
                     TASFFENKYPLSKKIIRGSRFSIAVLKGDEDYVALEALAKDVFLYYGLGCRSVSSLFV
                     PCGYDLTLLSVSFATMRSELTSDDYLAAYRYQKAVMTLESKNIVDGNFYILARGGNLP
                     PSMGVVNLFEYDNLDEIKEFVQTNRNRLQCVVNYDSNVSFGQAQFPGIEQYADNLNSL
                     EFLLQFV"
     gene            7250..7864
                     /locus_tag="P5293_03630"
     CDS             7250..7864
                     /locus_tag="P5293_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRR49024.1"
                     /translation="MEQIKIMDTFKSISKPASGIYKELGSKFLSFAYPVSSEEEIKDI
                     LAYLRKTYFDAKHHCYAYRLGKNGEKWKVSDDGEPSSTGGKPILGQLLSNELSDILIV
                     VVRYFGGTKLGLPGLMRAYRNAAADAVSNSEIIMKTVFKQLVVHYDYSLTHEIMQSIK
                     ENEAEIVDQRIEQNSILKLKIKENAFDSFCNKLSSFGATFEICN"
     gene            7888..8793
                     /gene="pyrB"
                     /locus_tag="P5293_03635"
     CDS             7888..8793
                     /gene="pyrB"
                     /locus_tag="P5293_03635"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRR49025.1"
                     /translation="MPKHLISIHDFSKEDILDVLRMAADFEKQPSQPIFHDKVIASIF
                     FEPSTRTRLSFETAANRLGARVIGFSDAGNTSVSKGETLKDTIMMVSNYADLIIMRHP
                     LEGSARYASEVSKVPVINAGDGANQHPSQTLLDLYSIQKTQGMLSNLKIDVVGDLKYG
                     RTVHSLLQAMSHFSPTFIFTAPEELKLPDEYKLFLKSKDIPFSETNELIYGFNDADII
                     YMTRVQRERFTDPIEYERVKNVYSLNLSMLEKTKPNMKILHPLPRVNEIAYDVDNSNH
                     AYYFEQAKNGVYTRMAIIAKLLGEK"
     gene            8803..9267
                     /gene="pyrI"
                     /locus_tag="P5293_03640"
     CDS             8803..9267
                     /gene="pyrI"
                     /locus_tag="P5293_03640"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRR49026.1"
                     /translation="MEKHLKVSAIKEGTVLDHIPPDQLFNVIDILGLAHSTNQMTFGT
                     NLDSKLLGKKAIIKIADRFFKDDDINKIALIAPQAKINIIHDFEVVEKKELSIPATIK
                     GIVKCMNPVCITNHQPVETLFTTIKENGELKLLCHYCEKATSEKNIKIISND"
     gene            9348..10421
                     /gene="serC"
                     /locus_tag="P5293_03645"
     CDS             9348..10421
                     /gene="serC"
                     /locus_tag="P5293_03645"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRR49027.1"
                     /translation="MKVAHNFNAGPCVLPKPAIKAAIEALNDYKGTGMSVIEVSHRSK
                     EWEATMEECRALWKELLNIPEGYHVLFLGGGASLQFLYVAMNLLENKAGYLETGVWAK
                     KALKEAQGIGNAFAVASSSDKNFNYIPKGYEIPKDLDYFHITTNNTIYGTEIHEDIDC
                     PVNLVADMSSDIMSRPVDVSKYALIYGGAQKNVGPAGVAFVIVREDILGKVSRYLPTM
                     LDYRTHIKEQSMFNTPPVFSIFVMTETLKWLKGFGGLQAIYRQNKEKAALLYNEIDRN
                     KMFVGTAAKEDRSLMNVCFVMAEGYKDKEDAFMAFAKERGMVGIKGHRSVGGFRASIY
                     NACPVESVKALISCMQEFEKLNS"
     gene            10426..11346
                     /locus_tag="P5293_03650"
     CDS             10426..11346
                     /locus_tag="P5293_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR49028.1"
                     /translation="MKVLVATEKPFAKVAVDGIRKIVEGAGYTLALLEKYTSSDEFYT
                     AVADADALIVRSDKVTKEVINAAKKLKIVVRAGAGYDNLDLNACSERGIVAMNTPGQN
                     SNAVAELAIGLMIYMSRNCFTPGTGVELKEKTLGIQAFGNVGRLVAELGKGFGMNVIA
                     FDPYVNAESIKSSGVTPVNNLDELYEHSNYLSIHIPATDQTKKSIGYDLINKMPKGAV
                     LVNTARKEVINEDGLIKVLEERSDLKYATDVAADNQAILNEKFGIRVYATPKKMGAET
                     AEANINAGLAAANQIIRFFTADDRTFQLNK"
     gene            11366..>12598
                     /locus_tag="P5293_03655"
     CDS             11366..>12598
                     /locus_tag="P5293_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRR49029.1"
                     /translation="MVKVKPFAAIRPPKEIAHEVASPPYDVLSSEEAKSLAGEKSLLH
                     IIKPEIDFDPIIDEHSQKAYDKAVENFKLWQQKGWLVQDKKEMYYVYAQTMGERTQYG
                     LVVGANVDDYLSGKIKKHELTRKDKEEDRMIHVRIQNANLEPVFFAYPDNDEINKTVS
                     EIVKSTPEYDFVAEDGFGHKLWLIEDNTTIKRITDIFSDIPAFYVADGHHRTAAAALV
                     GEEKRRNNPHHTGDEEYNYFLAVVFPESHLKIMDYNRVVKDLNGMTPSEFVEKLKYDF
                     TVEKKGSEIYRPDTFHNFSMYIDGCWYSLTAKPGKYDENDPVGVLDVTIISNLVFDKL
                     LDIKDLRTSKRIDFIGGIRGLGELKKRVDSGKMAAAFALYPVSMRQLIDIADTGNIMP
                     PKTTWFEPKLRSGLTIHKL"
BASE COUNT         4298 a         2447 c         2341 g         3512 t
ORIGIN      
        1 ctaatagcat ctacctggtt tttcataaga gcaataagcg gagatataat aacagccgta
       61 ccatccataa gtaaagccgg aagctgataa cacagagact tgccaccgcc cgtaggcatc
      121 aacacaaatg catcacctcc attaataaga tgattaataa tatcctcctg ctgccatttg
      181 aaagagtcaa acccaaaaaa cctcttcaac tgttcatgca aaaattcaga agtaatttgc
      241 atataataaa ataaggaata attgtctaaa aacagaacaa aataagttac ctttgcaata
      301 ctaagttatc cattagtttt tttatctcaa aatttttatg accatttcaa aagaagaact
      361 agcacaaatt gcacaggaag taattaataa ggaggcaact tcaatcgcac gccttgctgg
      421 tttcattgac agtcagttca tagatatact ccatttaata ttcaacagta agggaagatt
      481 gatagttaca ggcataggca aaagtgccat aattgccaac aaagttgtgg caactctcaa
      541 ttcaacaggt actcctgcca tatttatgca cgcagcagat gctattcatg gagatcttgg
      601 tattattcaa aaagatgata tagttctttg cctctcaaaa agcggtaata ctgccgaaat
      661 aaaggttttg gtaccatgga ttaagaaaat gggtaacaaa ataataggca tggtatccga
      721 caccaattca tttttggcaa aaaattcaga ttatcttatc agagcaacag tcgattctga
      781 agcctgtcct aacaatcttg ctcctacatc aagcacaact gctcagttag taatgggcga
      841 tgctcttgca gtttgtctac tcaaaatgag aggattttca caggaagact tcgcaaaagt
      901 ccacccggga ggttcactgg ggaaaaaact ctatacaaga gtatcggact tgatagacag
      961 atctgtaaaa ccaatggtta gagaagacga ttctatccag aatacaatat taaatatctc
     1021 ggccaacaga cttggagcca cagtggtatt gcatgaaaac ggagagatag caggtattat
     1081 tacagacgga gatgtaagaa gaatggttga aaaaggaatc gatatacaga cactccgagc
     1141 cagtgatata atgtcaccca accctaaaaa aattgatgca gaagaattgg ctgtaaaagc
     1201 tttcaacatg atggagcaga acaaaattac ctctttggtt gttattgaaa aaggaaaata
     1261 tataggactt gtacacattc acgacatact cagagaggga atagtatgac agaattcaat
     1321 cctaatgaca taggagtagc taatggcaat ttttttgggt tacctttcaa acccgaacaa
     1381 tctgagattg ttctaatctc tgttccatgg gatgtcacaa catcttatcg ttccggcacc
     1441 agctacggac cacaagctat aatgaatgca tcactgcagg tagatctgtt tgacaatcat
     1501 gttaaacgtg catgggaaac taaaattggt acaatacccg aagattattc aataagacaa
     1561 gataataaaa aagtacgcaa aatagcagaa aaggttattg aacatctcgc taaaggagaa
     1621 tctttgcaat cagtccagag tgaagtggat acagttaaca ctgcgtcagc ggctctaaac
     1681 agcaacattt acagaattag taaagactat atatctaaag ggttgactgc agggattgtg
     1741 ggtggagaac acagtgtgcc atttggatat ttcacagcac tgtctgaaat atacgaagat
     1801 tttggcatac ttcagataga tgctcatgca gatctgagga aagcatacga aggttttaaa
     1861 tattctcacg cctcaataat gtataatgct ttgaatgaaa tacctcaaat ttctcaactt
     1921 actcaagtag gcatcaggga ttattgtcag gaagaggcag atttcatcaa tagagaccca
     1981 cgcatcagaa gcttcactga ttatgaaatc cgcactaata tctttgaagg taaagtctgg
     2041 aaaagacagt gtgaagaaat catttcttct ctgcctcaaa acgtatacat aagttttgac
     2101 attgatggtc tttctcccga caattgcccc aatacaggta ctcctgtgcc gggaggactg
     2161 agctttatgg aagcagattt cctgctgtac atgctggctt tatccaacaa aaaaatcatc
     2221 ggttttgatt tatgtgaagt agcgccagct tctgacaacg aatgggatgc caatgtcgga
     2281 gcaagattac tttataaatt gtgtatttat tcacaatata attcaaaaag acaattagat
     2341 cgatgagcca gcaaacgact cctattacga agatcaaacg tccgtccacc aagtctgcat
     2401 ttagatcctt tctaaacaat cagcacagtg gaggaatagc attgctgatt tttgcatcta
     2461 tttcgattat tgcagccaat tttgaaccat taaatttttt aactaagctg tgggacagag
     2521 aaataggttt ttctttgggc agctttgagc ttaagatgtc tctcctgcac tggatcaacg
     2581 atggtttaat ggctctcttt ttcctttctg tgggtcttgg gataaaacgt gagcttgtta
     2641 caggggagct ttcatcaatt aaacgcgcgt ctctcccaat tatcgcggca ttaggaggca
     2701 tgatattccc cgctttaatt tattcggcat tcaattataa tactccgacc caaaatggat
     2761 ggggaatacc aatggctact gatattgctt ttgcaatagg agttatctca ttaatgaata
     2821 aaagagttcc ccattcattg aaagtttttc tcacagcttt ggccattgtc gacgatttag
     2881 gcgcaatcat cgtaatagcg atcttttatc caacacatga tattaacttt acaatgcttt
     2941 tatgggcatc tgccataatg ataatcctga tagccatgaa caagaagaat gttcaaaacg
     3001 ctgtttttta tattcttcca ggcttattgc tttggctttt catattgaaa tcgggaatac
     3061 attcaactat tgccggagta cttctagcta tgactatccc ggtaaaaatg aaagaagata
     3121 atttaacgaa gaatagagtt cctcttttaa agaaaatgga gagtttcatt actccaatag
     3181 ttacatttat aatagttcct gtgtttgccc ttgctaatgc aggagttaaa ttttcaatag
     3241 acttaagcgg accacttcag aatatctcaa aaggtatttt ttgtggcctg gtctttggta
     3301 aaccaattgg aatattcctc tcatcttatg tcgcctgtaa aataggtttg gccgatttac
     3361 caacaaattc caactggaaa atgttatttt cagtaggaat aattgctgga attggtttta
     3421 caatgtctat ctttatcgat aatttagcat ttgaagatcc tgacatagtt gcagcaggta
     3481 aaagagttgt tttgctttcc tcattaactg ctgccataat tggatcctta attctatacg
     3541 cttcaactaa aaaaacaatg gaaacaaaca aaccaaataa acaaaaaaat gaaaacaatt
     3601 aaatttattt ctgtaatctt aattgcactc cttgtgtcaa cttcttgtgg taaaaaggag
     3661 agcacaagca ctaacagtaa ttatggtatt tcccaatcag ctatcgattc agcaagttat
     3721 gcagtcggca tcactttggg ttcaatgatc aaacagtcca attttggtga actgaatctg
     3781 aaagaagtta ccaaggcaat gaaggccgtt attgacggag acagcctaaa aataagcatg
     3841 attaaagcta atgaagtgat ccagaatttc ttaatgaaaa gacaggaagc tattggcgca
     3901 gaaaataaaa ccaaaggtga agagttttta aaagccaatg caactaaaga cagtgttgta
     3961 acaacagcgt ctggtttgca atacatcatt atcaacccag gatctccgct taaacctaca
     4021 gaaacagata cagttcaggt tcattatgta ggaactttga ttgacggtac agaatttgat
     4081 tcttcataca agagaggaga gcctgcaaga tttccgctta acggtgtgat caaaggctgg
     4141 gtcgaaggtg ttcaacttat cggtgaaggt ggcaaaataa aactctttat cccatctgaa
     4201 cttgcatacg gcgctcagca agccggcccg caaattggtc caaactcaac acttatcttc
     4261 actattgacc tgctgaaggt tagtccaagc aaaccagttg cagagacaaa agctgctaaa
     4321 taaatgtggt tatggctatt gaaatcacag gtaaactgat aaaaaaactc cctcttcaat
     4381 ccggatctac tcctcgtgga gaatggaaga aacaggaatt tatcatcgaa acacaggaaa
     4441 attatccaaa aaagatctgt atgagtgtgt ggggagatga tggaatatca gacattgaac
     4501 gccattcaga aggagatatt ctcaaacttt cagtcaacat tgagtcaaga gaatataacc
     4561 aaagatggta tactgatgtc cgcgcgtgga gaattgagaa attatcaggc gaaaatactg
     4621 acaatagccc tcattacaat tctcaacctg aacatcagaa ttcatctcaa atctctaaca
     4681 gtgaaaatta tgattccgac tcagagatag atgatcttcc tttctaatta atagtcactc
     4741 tataacgaca tatggagctg aacaaaagaa tatttttttg agtcagctcc atttattttt
     4801 tatcttgagc gacgttttat ggattccgaa ggaataagac cttctcgtcg ccaccaagtt
     4861 atttgccttt tagcatagcg tcttgtattg cgtttaatca accaaaccgc ttcatcaaga
     4921 gaaaccttac catcaatata atcaaacagt tcagaatagc ctacagtctt taaggctgtc
     4981 cgttttcgaa actgatacag ccctgttgcc tcttcaagaa ggccagcttc catcatcttc
     5041 tctactctta gatttattct ttcatacaac tcttctctcg gcctgtcaac aactatcttt
     5101 tctatttcaa aattcctctt cttttgagta taacttttga aggatgaaaa ttttcttccg
     5161 gtcatcaaag taacctccaa agcccgtatt accctctgag gatttgtaat atctattgaa
     5221 tcgtagctct ctctgtccaa caacctcaat tcagatctaa gagaataaag tccctcagtt
     5281 ttcaatcgag acattaaggt cattcgtaac tctggatcgc tttttggaaa atcatccatg
     5341 cctttgcata tggcatcaac atagagaccg cttccgccaa ccatcaccag attgtcgtga
     5401 ttctcaaaca atgtttcgat aagaggtagt gcctccattt catattggcc ggcagtataa
     5461 tcatcgtgaa tagaatgtga aaaaataaaa tagtgtttaa cgagagataa ttgttcagga
     5521 gtaggaggcg cagtgccaat tttcatttct ctgtatatct ggcgggaatc gcaagaaata
     5581 acaggagaac caaacttaag agctagtgaa atggcataat cagttttgcc ggcagcagtt
     5641 ggtccgacga tgatagtcaa cctctttcta atcatcagta tcttcctgac ctcctaaaaa
     5701 atcttcagag tcagaatcgc ccatttcaat catctgttca aagtcgtcat aattatcaga
     5761 aaactgatcc ggatttttcc ctctttctgc cacaagtctg ggataagagc gtttcagaga
     5821 ctcttcttca gttgaaagaa attccaagtc tatagccttg tctttataga tatcatagac
     5881 atactgaagt tttacctcac ctttattcac aactttttca actgatatag aatccatagc
     5941 gccatctcca agatcaaaaa gaccatattc actttttacc ttaccttcgc cattcaatcc
     6001 tctgaaaacc accatttggt caggagcaaa acccaagtca ttctgcaaaa acagatgtaa
     6061 tgaatataaa gaagtattgc ctttaatctc atactccctc atgaaaatct tgttaccagc
     6121 aattgacgct ttgaacctgt aaaccataac atgaagattc tttacgcaaa gttaaacaaa
     6181 ttgcagtaaa aattccaaag aatttaaatt gtctgcatac tgctctatac caggaaattg
     6241 agcttgccca aatgaaacgt tgctgtcata attcactaca cattgaagcc gatttctatt
     6301 agtttgaaca aactccttta tctcatctaa attgtcatat tcaaacaaat tgactactcc
     6361 catagatgga ggtaaattgc ctcctcttgc aagaatgtaa aaattaccat ccacaatatt
     6421 tttagattct aaagtcataa cagccttttg atatctataa gcggccaaat aatcatccga
     6481 cgtcagttct gacctcattg ttgcaaatga gactgacaaa agagttaaat catatccaca
     6541 tggcacaaac agagagctca cacttctaca cccaagaccg taatataaaa aaacgtcctt
     6601 tgccagagcc tcaagtgcaa catagtcttc atctcccttc aaaactgcaa tactaaaccg
     6661 actgcccctg ataatttttt tggataaagg atatttattc tcaaaaaagg aagcagtgtt
     6721 atctgaacct gatgcaatta aaatatctgc acataaaggt aaatagtcaa taatttccac
     6781 tctatcactc caataatcat ctatttcaaa gagtactcca agaatatatg gtaacaaata
     6841 agaatctttg gaagaaagtt ttattttcgc tctaaaccct gctgcaagaa tagtcataaa
     6901 atcgtgaaat ccgacaagag gaatgttccc tgccatcact gccagtactt ctccttggcg
     6961 ttcttctatt ctgtcagagt aatctaaaag ccatttttca agtttggatt tatcaagaaa
     7021 acaattacaa atagaagtca gggcaatatg ctgcatattc aaagtaaaaa gaggattacc
     7081 cttaaaagag tttctcaatg cagcttcaaa ttttctatca aaaaccgatc cgctcagagt
     7141 cgctttcaat ctatcaccca gggcagaaaa atcatcaata aaaactttac tatacatttg
     7201 gcaaatttag caaaaggggc tacttttaca aaacgcaaag tttgttcaaa tggaacaaat
     7261 caagatcatg gatacattca agtcaatatc caagcctgct tcaggtatat ataaagaact
     7321 tggcagtaaa tttctctcat tcgcataccc tgtgagttct gaagaagaaa ttaaagacat
     7381 actggcatac ttgaggaaga catatttcga tgccaaacac cattgttacg catacagact
     7441 tggcaaaaat ggagaaaaat ggaaagtcag tgatgacggt gaaccatctt caacaggtgg
     7501 caagcctatc cttgggcaat tgctctcaaa cgaattgagc gacattctta tcgtagttgt
     7561 gagatacttt ggaggcacaa aattaggact gcccggactg atgagggctt acagaaatgc
     7621 agcagctgat gctgtttcca acagtgagat aataatgaaa acagttttca aacaactggt
     7681 tgtgcattac gattactcac ttacgcacga gattatgcaa tcaattaagg agaacgaagc
     7741 agaaatagta gaccaacgca ttgagcaaaa cagtattctt aagctcaaaa tcaaagaaaa
     7801 tgcatttgat tctttctgca ataaactctc gtcatttggt gcaacttttg aaatatgcaa
     7861 ctaatcttct aacacaaata taattatatg ccaaaacacc ttatatccat ccacgatttc
     7921 tccaaagagg atatcctgga tgttcttcgg atggctgcag attttgaaaa acaaccatca
     7981 cagcctattt tccacgataa ggtaattgca agcatctttt ttgaaccatc taccagaacg
     8041 agactaagtt tcgaaacagc agctaacagg cttggtgcga gggtaatagg tttttccgat
     8101 gcaggcaata caagtgtgtc aaaaggagaa actcttaaag acactataat gatggtctct
     8161 aattatgcag atctcataat tatgagacat ccgctcgaag gcagcgcaag atatgcatct
     8221 gaagtttcaa aagtcccagt aataaatgca ggagatggag ccaaccaaca tccatcgcaa
     8281 acattgctag atctttactc gattcaaaaa acacaaggaa tgctttcaaa cctcaaaatt
     8341 gatgtggttg gagatctgaa atacggaaga acagttcatt ctcttcttca agccatgtca
     8401 catttctctc ctacatttat ctttactgcg cctgaagaac ttaaacttcc cgacgaatac
     8461 aaattatttt taaaaagcaa agatattccc ttcagtgaga ctaatgaatt gatttacggc
     8521 tttaatgatg cagatattat ttacatgaca agagtacaga gagaacgatt tactgatcct
     8581 atagaatatg aaagagttaa aaacgtttat agtctaaatc tttccatgct tgaaaaaact
     8641 aaaccaaaca tgaaaattct tcacccactt ccaagagtaa acgaaattgc atatgatgta
     8701 gataacagca atcacgccta ttattttgaa caagccaaaa acggcgtata taccagaatg
     8761 gccattatag ctaaactatt aggagaaaaa taatttcaaa caatggaaaa acatctaaaa
     8821 gtaagtgcga taaaggaagg aactgtgcta gaccatattc ctccagacca gctattcaat
     8881 gtaattgaca tattggggct tgctcatagc acaaaccaaa tgacatttgg aacaaatctc
     8941 gatagcaaac tgcttggaaa aaaagcaata ataaaaatag ccgatcgttt cttcaaagac
     9001 gacgatatca acaaaatagc actcattgct cctcaagcca agataaacat catacacgac
     9061 ttcgaagtcg tagaaaagaa agagctttcg atacctgcga ctattaaggg aatagttaaa
     9121 tgcatgaatc ccgtatgcat aacaaatcat caacctgtag aaaccctatt cacaacaata
     9181 aaggaaaacg gggagctgaa acttctctgt cattactgtg agaaagcaac aagtgaaaaa
     9241 aacatcaaaa tcataagtaa tgattaacaa ttagcttaca aattgttaat ttttgtctat
     9301 ctttgaaaca cattaaaatt aaatgttgaa ttaatatata ttatatcatg aaagtcgctc
     9361 acaactttaa tgcaggccct tgtgtcctgc caaaaccagc catcaaggct gcaattgaag
     9421 cccttaatga ctataagggt acaggcatgt cagttatcga ggtatcacac cgttcaaaag
     9481 agtgggaagc aacaatggaa gagtgtcgtg cactatggaa agagctactt aacattcctg
     9541 aagggtatca tgtattgttc cttggaggag gcgcaagtct tcaattcctg tacgttgcaa
     9601 tgaacctgct ggaaaataaa gcaggttatc tcgaaacagg tgtatgggca aagaaagcac
     9661 ttaaggaggc ccaaggtatc ggtaatgcct ttgctgttgc ctcatcctca gataaaaact
     9721 tcaactatat acctaaagga tatgagattc ctaaagattt ggattatttc cacattacaa
     9781 caaacaacac tatttacggt actgagattc acgaagacat agactgccct gtcaaccttg
     9841 tagctgatat gtcatccgac attatgagtc gtccggttga tgtaagcaag tatgcactta
     9901 tttatggagg agcccagaaa aatgttggcc ctgccggcgt tgcattcgta atagttcgtg
     9961 aagatatttt aggcaaagta tctcgctacc tgcctacaat gctcgactac agaactcaca
    10021 taaaggaaca gtcaatgttc aatactccgc ctgtattctc aatttttgtt atgacagaga
    10081 ctctcaaatg gctcaaggga tttggcggtc tccaggcaat atacaggcaa aataaagaaa
    10141 aggcagctct tctgtacaat gagatagatc gtaacaaaat gtttgttggc actgcagcca
    10201 aagaggacag atcactgatg aatgtatgct ttgtgatggc agaaggttac aaagacaaag
    10261 aagatgcttt catggcattt gccaaagaga gaggaatggt tggtatcaag ggacaccgtt
    10321 cagtaggagg tttccgtgct tcaatataca acgcatgccc tgttgaaagc gttaaagcat
    10381 tgatttcatg tatgcaggaa tttgaaaaat taaacagtta aggttatgaa agtattagtt
    10441 gccacagaaa aaccatttgc taaagttgca gttgacggaa taagaaaaat agttgaaggt
    10501 gcaggttaca ctttagcact actcgaaaaa tatacttcaa gtgatgaatt ttacacagca
    10561 gtagcagacg cagatgcatt aatcgtccgc tccgacaagg tgacaaaaga ggtaataaac
    10621 gctgcaaaaa agttgaaaat tgttgtacgc gcaggcgccg gatatgataa tcttgacctt
    10681 aatgcctgca gtgaaagagg gattgtagcc atgaacaccc ctggacagaa ttctaatgct
    10741 gttgcagagc ttgcaatagg gttgatgatc tacatgtcaa gaaactgttt tactccgggc
    10801 acaggtgttg agctaaagga gaagactctt ggtatccagg cttttggcaa tgtaggacgg
    10861 cttgttgccg aattgggaaa gggatttggc atgaatgtca tagcatttga tccatatgta
    10921 aatgctgaat caattaaaag ctccggtgta actccggtta ataatctcga tgaactttac
    10981 gaacattcaa attatctttc aattcatata cctgccactg atcaaactaa aaaatcaata
    11041 ggttacgacc ttattaataa aatgcctaag ggagccgttt tagtaaacac agcccgaaaa
    11101 gaggtaataa atgaagatgg actcattaaa gttctcgaag agagaagcga tcttaaatat
    11161 gcaacagatg ttgctgcaga caaccaggca atactcaacg aaaagtttgg tataagagtc
    11221 tatgctactc ctaaaaaaat gggagcagaa actgcagaag caaacatcaa tgccggactt
    11281 gctgctgcaa atcagataat aagatttttc actgcggatg acagaacatt tcaacttaac
    11341 aaatagagta aacagtaaag ataatatggt aaaagttaaa ccttttgcag ctatacgccc
    11401 tccaaaagag attgcacacg aagtagcatc acctccctat gatgtcctta gctccgaaga
    11461 ggcaaaatca cttgcaggag agaaatctct gttacatatc attaaacctg agattgattt
    11521 tgaccctata attgatgaac attcccagaa agcctatgac aaggctgttg aaaatttcaa
    11581 attatggcag cagaaaggat ggctggttca agacaaaaaa gagatgtatt atgtttacgc
    11641 tcaaacaatg ggagaaagga ctcagtacgg acttgttgtt ggagccaacg ttgatgacta
    11701 tctttcagga aagatcaaaa agcacgaact caccagaaag gataaagaag aagaccgaat
    11761 gattcatgtt cgcattcaaa atgccaatct tgaacccgtt ttctttgcat atcccgacaa
    11821 tgacgagatt aataaaacgg tatcggaaat tgttaagagt acacctgaat acgattttgt
    11881 agcagaagat ggttttggac acaaattatg gctaatagaa gacaatacaa ctatcaaaag
    11941 aataactgat atattctctg atataccagc attttacgtt gccgatggac atcacagaac
    12001 agctgctgca gcactcgttg gagaggaaaa gagaagaaac aatcctcacc acacaggaga
    12061 tgaagaatat aactattttc tcgctgttgt ctttccggaa agtcatctta aaattatgga
    12121 ctacaatcgt gtagttaaag accttaacgg tatgacacct tccgagttcg ttgaaaaact
    12181 taaatatgat ttcacggtcg agaaaaaagg aagtgaaatt tacaggccag atacatttca
    12241 caatttttcc atgtatattg atggttgctg gtattcactc acagcaaaac ccggaaaata
    12301 tgatgagaat gatcctgtcg gagtgctcga tgtcacaatt atttcaaatc ttgtgttcga
    12361 caagcttttg gatattaaag atctgagaac atcaaaacgt attgacttca tcggaggtat
    12421 ccgaggttta ggtgagctga agaaacgcgt tgactcaggt aaaatggctg ctgcctttgc
    12481 tttatatcct gtatctatga gacagcttat cgatatagca gatactggca atataatgcc
    12541 gccaaagaca acctggttcg agccaaaact gagaagcggg cttactattc acaagctt
//
LOCUS       DAOTQL010000038        12052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4867_length_12052_cov_5.286738, whole genome shotgun sequence.
ACCESSION   DAOTQL010000038 DAOTQL010000000
VERSION     DAOTQL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4867_length_12052_cov_5.286738"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..1035
                     /locus_tag="P5293_03660"
     CDS             292..1035
                     /locus_tag="P5293_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRR49030.1"
                     /translation="MDDKKKILVVDDEEDVCEILKFNLVNSGYEVDTAYSSEEALIKL
                     AKNFDLLLLDVMLGGISGFTLADVIRKELHNEVPIIFITAKDTVDDKLKGFNVGADDY
                     ILKPFSIKEVMARIQAVLSRTALYKKAEEERREYNKKHPGHQVIEISGLKIDSGSKQV
                     IVNGTTLSLTKKEYEILYMLASSPFKFYSRQEILDTVWENEVYVLERTVDVHIARLRK
                     KLGFYGSMIINRSGFGYCFNPNEIEKDKR"
     gene            1010..2023
                     /locus_tag="P5293_03665"
     CDS             1010..2023
                     /locus_tag="P5293_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRR49031.1"
                     /translation="MKLKRIKDNNSGVFLVSLALLVVAGVIAYLLIIYHERYLLGILF
                     LLAISIGSVVFFNSKVNKPIRDFKEFLNLLQKGHGNYENISFSKNELGEIEEKIIQTF
                     KQLEETKRYKQQLTHNIAHELKTPVTSIRGYLETLMQQEMDKDQRKFFIERAYMQTLR
                     LSSLITDISTLNNIEEASWKYETEKINIKKCIEEIESDLSFSLSENNIKVYTDVSDNI
                     EIEGVYILIYSLFKNLIDNSIEHAGRDVEIHIQTTKIDPDIIWFRYYDTGKGVSEEHL
                     ERLFERFYRVEAGRSRKSGGSGLGLSIVKNSVLLHNGSITVCNREEGGLQFDFSLSAK
                     MYQ"
     gene            complement(2001..2600)
                     /gene="folE"
                     /locus_tag="P5293_03670"
     CDS             complement(2001..2600)
                     /gene="folE"
                     /locus_tag="P5293_03670"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRR49032.1"
                     /translation="MAYTKIEHYDEEVTQRLAAHFREILSLIGEDPAREGIKLTPIRV
                     ARSVQFLTQGYEMDGTAILSSAMFKEDYKQMVIVKDIELYSLCEHHMLPFFGKAHVAY
                     IPNGHITGLSKIARVVEAYARRLQVQERLTVQIRDCIQSTLNPLGVAVVIEASHMCMQ
                     IRGVQKQNSVTTTSAFTGAFLKDIKTREEFMSLIGTSLH"
     gene            2657..3325
                     /gene="mqnB"
                     /locus_tag="P5293_03675"
     CDS             2657..3325
                     /gene="mqnB"
                     /locus_tag="P5293_03675"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRR49033.1"
                     /translation="MKILITAAEEEELITAKQAYNSLPLEIKERLDITFMLTGIGCTS
                     TSYRLTKMLCSSGPFNFALNIGIAGSFTESFPIGSVAMIDKEYFGDLGFETYFGFQTL
                     FDYNALNADSFPFKGGALYCKDIPPEIENALSSYKRATGVTVQTVSGLPEKKELLKNI
                     FSPEIESMEGAAFFYVCLHEKVQFAEIRSVSNFVGERDRKKWDIPLALDSLREAVRKM
                     LVAL"
     gene            3322..4155
                     /locus_tag="P5293_03680"
     CDS             3322..4155
                     /locus_tag="P5293_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRR49034.1"
                     /translation="MKLKIAYSSCPNDTFAFHAMINELIDTEGIEFSVDIADVEQLNK
                     RAARGIYDVCKLSYSTFFSICDRYVMLRAGSALGYDNGPLFVSLTNNVSKENLADLKV
                     AVPGEGTTGALLLRTAFPECKNLIPVLFSDIEKGILEGEFDAGVLIHEGRFTYRDKGL
                     NLIEDLGSYWQRSTGLPVPLGGIAVSRSIENDIRKKINRILKRSIEFALKNPDKSAEY
                     VCENAQEMDTEIQRKHIDLFVNEYTLDIGDIGQKAVETLYETASRLRPDIKNEKLLFI
                     E"
     gene            4172..4873
                     /locus_tag="P5293_03685"
     CDS             4172..4873
                     /locus_tag="P5293_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR49035.1"
                     /translation="MNQEVIKIKGIKKIYKVGNQEVKALNGIDIIINRNEYVAIMGPS
                     GSGKSTMMNILGCLDSPTSGEYILNGTDVSKMDDNELAVIRNREIGFIFQSFNLLPRY
                     TALDNVALPLIYSGESKENREERAKVALASVELTDRMHHKPNELSGGQRQRVAVARAL
                     INNPSIILADEPTGNLDTKTSVDIMKLFEEIHKKGNTIIIVTHEEDIAKHAKRIIRIR
                     DGVIESDHFNEHQII"
     gene            4900..5445
                     /locus_tag="P5293_03690"
     CDS             4900..5445
                     /locus_tag="P5293_03690"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRR49036.1"
                     /translation="MKIYTKTGDKGTTSLVGGTRVNKRDSRVNAYGTVDELISYIALL
                     RAVTVEDLYTDNLRRIQSDLMLAASHLAADEKGASKLKLLEENEITFLEEEIDKISNK
                     LPQQKAFIIPGPHKASAICHIARTVCRRAERAVLLIEEEIFEPRIEQCIKFLNRLSDY
                     LFIYARHLSINGNVPDDFWVQ"
     gene            5531..5752
                     /locus_tag="P5293_03695"
     CDS             5531..5752
                     /locus_tag="P5293_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRR49037.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYATRSGAPLEVIENLEDLE
                     DDGEIYESIEDIWPDYPTKDDFFFNEDEY"
     gene            5775..7124
                     /gene="trkA"
                     /locus_tag="P5293_03700"
     CDS             5775..7124
                     /gene="trkA"
                     /locus_tag="P5293_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRR49038.1"
                     /translation="MKIIIAGAGEVGSHLAKMLSNEYHDLTIIDNDEARLNRVAESAD
                     VVTIFGSPTSISTLSKAGAASADLFIAVSPAQEQDVNIISSLLAKKMGSTRVTARINN
                     DEYLENDNKILFTEMGIDLLFYPEKIASHEIIDLLKQTGTSEFMDFSGGKLQLIAFRL
                     DDGAPLIDKTIGELSSSNEEPLYRAVAVAREGQTIIPRRDTKFKEHDLVFVISKRAGV
                     HEVMAYSGKNNINVRRLMIVGGGRIGEMVAKKLENSVDYIKLIELRKERCELLNEKLS
                     KTLVINGDARNTDLLIEEDVNEFDAFVAVTSSSETNILSCVMAKKMGVAKTIAEVENI
                     EYIKLAENMGVDAVINKKLITASRIFRFTLSNKVQSIKCLNGSDAEILEFIVNPNSLI
                     TKDKIRNLGFPKDAIIGGVIRGNHSYIALGDTEIKAYDRVVVFALPSALKKVNKFFI"
     gene            7138..8997
                     /locus_tag="P5293_03705"
     CDS             7138..8997
                     /locus_tag="P5293_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRR49039.1"
                     /translation="MATILQAENISKSYGTRILFNNLEININEGDRIALIAPNGSGKS
                     TLLRILAGKDSSDIGGSVRCRESVKIAWLEQDPSLNPERDVFEEVFASSDELSKTVEE
                     YERAILSNNTDIIGSAIREMDRLNGWQYELKVKQILSLLKIDNLNQKVATLSGGQRKR
                     VAIAGIMLANADLLVLDEPTNHLDLEVIEYLEEYLCKPSLSLLMVTHDRYFLDRVCTQ
                     IMELDNGQLYRYQGNYSYYLQKKEERIENFNSETDRARNLFRKELEWIRRMPQARATK
                     AKYRINAFEQIKERALQKKVNHKIAIDVKSERLGSKIINCKGVTHHFGDYCTIDNFSY
                     NFAKGEKVGLVGPNGVGKTTFLNIISELIPPQSGIIETGETVVKGYYKQSGLEYTPEE
                     TVIDVVRKYSEIATLSDGSIIPVTQFLSKFLFPYSCHNTPVSRLSGGERRRLYLVTIL
                     MKNPNFLILDEPTNDLDIITLNVLEDYLSDFKGCLIIVSHDRFFLDKLADHLFVFMGN
                     GIIKDFPGNYSDYKEYCRNLEKEKNTTSKKIEERKQPSLKKANNKYSYKEKFEITTLE
                     TEINLLEKEKAEIEEILSTGHLSQKELTEKSKRIGEIISLIDQKSDRWIELNS"
     gene            complement(9009..10685)
                     /gene="ettA"
                     /locus_tag="P5293_03710"
     CDS             complement(9009..10685)
                     /gene="ettA"
                     /locus_tag="P5293_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRR49040.1"
                     /translation="MAEEKIIFSMNGVGKILPNSNKVILKDIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGIENDFQGEVVWAPGYSVGYLEQEPKLDDNKTVIEIVQEGVKEVVE
                     LMKEYEAVNNRFAEPMDEEEMNRLIEYQGELTDKIDQINGWDIDSKLERAMDALQCPE
                     PMASVKTLSGGERRRVALCRLLLREPDVLLLDEPTNHLDAESIQWLEAHLQQYRGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSVRLAMEEKQESKRRKTLER
                     ELEWVRMSPKARQAKSKARLSQYEKMLNEDGKSKDEKLEIFIPNGPRLGDKVIEALDV
                     SKSFGERLLFENLNFKLPPAAIVGVIGPNGAGKTTLFRMIMGYEKPDKGCFTIGETVK
                     LAYVDQQHKQIDPDKTVYQTIAENSEFIKLGNREVNARAYVSRFNFSGADQEKKCGIL
                     SGGERNRLHLALTLKEEANVLLLDEPTNDVDVNTIRALEDGLENFAGCAVVISHDRWF
                     LDRIATHILAFEGDANVYFFEGSYSEYEENKRRRLGDSEPKRFKYKKLIG"
     gene            complement(10717..12003)
                     /locus_tag="P5293_03715"
     CDS             complement(10717..12003)
                     /locus_tag="P5293_03715"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRR49041.1"
                     /translation="MKQLPYFLPVTIFFIALFLINTTVTSCSEGGKENSKDEESPGEV
                     ITEFGIPVSMFDVQEGSVKKGQFFSKLMSDLGVSNTDAYALAEASKGVFDLKKLKVGK
                     NYRAYFTRDEVPRLAYLVYEETPTSYFVFGLNDSIFVKSFNEQTETRLRVADILIENS
                     LWADVQRAGLSFEIAARLENIFAWSVDFFGLQKGDCFKVLYDEILIKNNVVGIGTVYA
                     STFTSKGKVYDAYLFQEGDTNAIQYFNSSGENLKKAFLKAPLSYTRVSSGFTYARRHP
                     ITRIVRPHTGVDYAAPKGTPVMSIGDGVVIQKGYSGGGGNTVKIKHNATFTTAYMHLS
                     KYGAGIAKGVRVRQGQVIGYVGSTGLSTGPHLDFRVWKNGKPVNPLNMESPPAKSVDK
                     AHMELFRATIAQRKHQIDSIISAQYVDTLVNNLGKM"
BASE COUNT         4062 a         2285 c         2202 g         3503 t
ORIGIN      
        1 cctgtatttt tttcaaccac tgttccggtc agaatagaat aattttgtgc taaagtcgtt
       61 ttaaagaaaa acattattaa taataaagag aaaattgtgg ttcttttaaa taatctgttc
      121 actgtttata ctttttaaat tctgacgaca aagctaaaag tgacatgtta cgatatcatg
      181 acaaaaccat taaaaaataa ttaagaaata tttaagaaaa tattaagaaa aaaacattta
      241 taaagtgttt aaataaatat ggttattttt gaaaaataat tgcatcaacg tatggatgac
      301 aaaaaaaaaa tcctggtagt agatgatgaa gaggatgttt gcgaaatatt gaaattcaat
      361 cttgtaaact ccggctatga agttgacaca gcctattcat ctgaagaagc tttgataaaa
      421 ctggcaaaaa atttcgatct gctactactt gacgtcatgc tgggcggaat ttccggtttt
      481 acattggctg atgtgatcag aaaagagttg cacaacgaag tacctattat cttcataacc
      541 gctaaagata ctgttgatga caaacttaag ggatttaatg tcggagctga tgattatatt
      601 cttaaaccat tctcaattaa agaagtaatg gcacgaatac aggcagttct aagcaggact
      661 gctttatata aaaaggcaga agaagaaaga agagagtaca acaaaaaaca tcctgggcac
      721 caagttatag aaatatctgg acttaagatt gatagtggct caaaacaggt aatcgtaaat
      781 ggtacaactc tttcgctaac taaaaaggaa tatgaaattc tttacatgct cgcatcttct
      841 ccttttaaat tttattcaag gcaggagatt cttgataccg tatgggaaaa tgaggtttac
      901 gtcctggaaa ggactgtgga tgtacatatc gctcgattaa ggaaaaaact cggcttttat
      961 ggatcaatga ttatcaacag atccggtttc ggatattgtt ttaatccaaa tgaaattgaa
     1021 aaggataaaa gataacaaca gtggggtttt tttagtatct ctggcgcttc ttgtggtagc
     1081 cggagtaatt gcttatttac taattatata ccatgaaaga tatttattag gtatactttt
     1141 tcttttggct atctctatag gatcagtcgt cttcttcaac agcaaagtca ataaaccaat
     1201 cagagatttt aaagagtttc tgaacctcct tcagaaagga cacggaaact acgaaaacat
     1261 aagtttttca aaaaatgaac tgggagaaat agaagaaaaa ataattcaga catttaaaca
     1321 acttgaagaa acaaagaggt acaagcagca acttactcat aatattgcac atgaactcaa
     1381 gactcctgtc acaagtataa gaggttatct tgaaaccctg atgcagcaag agatggataa
     1441 agaccaaaga aaattcttta tagaaagagc ttatatgcag actttaagac tctcttcgct
     1501 aattacggat atttctactc ttaataatat agaagaggcc tcttggaaat atgaaacgga
     1561 gaaaattaat attaaaaaat gcatagaaga gattgagagt gacttatctt tcagtctttc
     1621 tgaaaataat atcaaagtat atacagatgt aagtgataat attgagattg aaggtgtgta
     1681 tatactaata tattctcttt tcaaaaatct cattgacaat tcaatagaac atgcaggaag
     1741 agatgtggag atacatatcc agactacaaa aattgatcct gatattatct ggttcagata
     1801 ttatgacacc ggcaaaggag tttctgaaga acatctcgaa agattgttcg aaagatttta
     1861 cagggttgaa gctggaagaa gtagaaaaag tggaggctcc gggctcggtc tttcgattgt
     1921 caaaaattct gttctactgc acaatggttc cattaccgtc tgcaacagag aggaaggagg
     1981 ccttcagttt gactttagcc ttagtgcaaa gatgtaccaa taagactcat gaactcttct
     2041 cttgttttaa tatctttaag aaaggctcca gtaaaagcag aagtagttgt tacagaattt
     2101 tgtttctgca cccctcttat ttgcatgcac atatgagatg cttcaatgac tactgcaact
     2161 ccaagaggat ttaaagtgct ctggatgcag tccctaatct gaaccgtaag ccgctcctgt
     2221 acctgaagtc tccttgcgta tgcttcaaca acccttgcaa ttttactgag ccctgtaata
     2281 tgtccatttg gaatgtatgc aacatgtgcc ttaccaaaaa aaggaagcat atggtgttca
     2341 cacagtgaat aaagctcaat atccttaaca ataaccatct gtttgtaatc ttctttaaac
     2401 atagcagagc taagtattgc cgtcccgtcc atctcatatc cctgagtaag aaactgtacg
     2461 gatctggcca ctcttattgg tgtcaactta atcccttctc ttgcaggatc ctctcctatc
     2521 aaacttagaa tctctctgaa atgagctgcc aatctttgtg tgacctcctc gtcatagtgt
     2581 tcaatctttg tatatgccat aattattatt aatttcgggc aaaattagaa aattagatag
     2641 agaaacatct ctcataatga aaattttaat aactgcagca gaagaggaag aacttattac
     2701 agccaaacag gcttacaact cacttccctt agaaatcaaa gaaaggcttg atattacatt
     2761 tatgcttacc ggcattggct gcacctctac aagttacaga cttaccaaaa tgctttgcag
     2821 ttccggacca tttaattttg ctctaaatat tgggattgct ggaagtttta cagaatcatt
     2881 ccctatagga tctgttgcta tgatagacaa agagtacttt ggtgatctgg gatttgaaac
     2941 atactttggt tttcaaactc ttttcgatta taatgctcta aatgcagatt ctttcccatt
     3001 caagggtgga gcactctact gcaaagatat tcctcctgaa attgagaacg cactctcttc
     3061 atacaaaaga gctaccggag ttacagtaca aactgtaagt ggacttcctg aaaaaaaaga
     3121 acttctgaaa aatatttttt cacctgaaat tgagtccatg gaaggagcag ccttttttta
     3181 cgtctgtctt catgagaaag tacaattcgc agaaattcgt tcagtatcca acttcgttgg
     3241 agaacgtgac agaaaaaaat gggatattcc tctggctctt gactctctaa gagaagctgt
     3301 cagaaaaatg cttgttgcct tatgaagcta aagattgcct attcatcatg ccctaatgac
     3361 acctttgctt tccacgcaat gatcaacgag ttaatagata cggagggcat tgagttttca
     3421 gttgacatag ccgacgtgga acaattgaac aaaagggctg cccgtggcat ttatgacgtg
     3481 tgcaaattaa gttacagtac tttcttttct atttgcgaca ggtatgtaat gttgagagcc
     3541 ggaagtgctc taggttatga taacggcccc ttgttcgttt ctctcacaaa taatgtcagt
     3601 aaagaaaatc ttgctgattt aaaagttgct gttcctggag aaggtacgac aggtgctctt
     3661 ttgttaagaa cagcgttccc ggagtgcaaa aatcttatac cagtactttt ctctgacata
     3721 gaaaaaggta ttcttgaagg agagtttgat gcaggggtgc ttatacatga aggaagattc
     3781 acatacagag ataaaggttt gaatctcatt gaagatctgg gaagctattg gcaacgttct
     3841 acaggattac cggtaccatt aggaggaata gctgtatcaa gatctattga aaacgacatt
     3901 cggaaaaaaa ttaacagaat cctaaaacga agcatagaat ttgcattgaa aaatcccgac
     3961 aaatctgcag agtacgtttg tgagaatgct caagaaatgg atacggaaat tcaaaggaaa
     4021 cacattgatc tatttgtaaa tgagtatact ctcgatattg gagatattgg tcaaaaagct
     4081 gtagagacac tctatgaaac agcttcccgg cttagaccgg acattaaaaa tgaaaaactg
     4141 ctttttattg aataaataaa tcaaaaacgt catgaatcag gaggtaatca aaataaaagg
     4201 gatcaaaaag atctacaagg taggaaatca ggaagttaaa gctctcaatg gtattgatat
     4261 aattattaat cgaaatgaat atgttgccat aatggggccc tccggttcag gaaaatccac
     4321 aatgatgaat attcttggat gccttgacag tccaacatca ggagagtata ttttaaacgg
     4381 aacagatgtc agcaaaatgg acgataatga actggccgtc ataagaaata gggagatagg
     4441 ctttatattt caatctttca atcttttacc tagatatact gctcttgaca atgtggcctt
     4501 acccctcatt tattcaggag agtcaaaaga aaacagagaa gagagggcaa aagttgctct
     4561 ggccagtgta gaattgactg acagaatgca ccataaacca aatgaactat caggaggtca
     4621 aagacaaaga gttgctgttg ccagagctct tatcaacaac ccatcaataa ttttggcgga
     4681 tgaacctacc ggcaaccttg ataccaaaac ctctgtggac attatgaaac tatttgaaga
     4741 gattcacaaa aaaggcaata caataattat cgtgactcat gaggaagata tagccaaaca
     4801 tgcaaaaaga ataatccgaa taagagacgg cgtcattgaa tcagatcact ttaacgaaca
     4861 tcagattatt taaaagttaa taatcagcat aaatcaataa tgaaaatata tacaaaaaca
     4921 ggcgacaaag gtacaacctc tcttgtcgga ggaacaaggg taaataagag ggattccaga
     4981 gtcaatgcat acggaactgt agacgaactt atttcatata ttgctcttct tagagcagta
     5041 acagttgaag acttatatac agacaatctg cgcagaatac agtctgatct tatgctggca
     5101 gcttctcacc ttgccgcaga tgagaaagga gcatctaaac ttaagttgct tgaagaaaat
     5161 gagataacat tcctcgaaga agaaattgat aaaatatcaa acaagcttcc tcaacagaaa
     5221 gcatttatta ttcccggacc acataaagcc tctgcaatct gtcacatagc aagaactgta
     5281 tgccgcagag cagagcgggc agtactccta attgaagagg aaatatttga accaagaatt
     5341 gaacaatgta ttaaattttt aaacagactc tccgattatc tgtttatcta tgcaagacat
     5401 ttatcaataa atggaaatgt gcctgatgat ttttgggttc aataaatgaa gaaaatcaac
     5461 aattaattac taccttcgca cactattatt gaataatata gtaaatgttt aattattaat
     5521 cgtttacgtt atgtattgga cacttgaact ggcatctaaa cttgaagatg ctccatggcc
     5581 tgcaaccaaa gaagagttga ttgactatgc aactcgttcc ggagcacctc ttgaagtgat
     5641 tgagaacctc gaagatctgg aagatgacgg agagatttac gaaagtattg aggacatttg
     5701 gcctgactat cctaccaaag atgacttctt ttttaatgag gacgaatact aaaaaataga
     5761 aagaatcact caaaatgaaa attattattg ccggggcagg agaagttgga agccacctgg
     5821 ctaagatgct cagcaatgag tatcacgatc ttactataat cgacaacgac gaagcaaggc
     5881 tcaatcgtgt agccgaaagt gctgatgtgg tgaccatttt tgggtcacct acgtctataa
     5941 gtacactttc aaaagcaggt gctgccagtg cagatctatt tattgccgta agccctgcac
     6001 aggagcagga tgtaaatatc atcagctctc tactggctaa aaaaatgggc agtacccgtg
     6061 tcactgcaag aattaataac gacgagtacc ttgaaaacga caataagatt ctttttactg
     6121 aaatgggtat tgacctttta ttttatcctg aaaagattgc ttctcacgaa ataattgatc
     6181 ttctcaaaca gacaggcacc tcagagttta tggatttttc aggtggcaaa cttcaattga
     6241 ttgctttcag acttgacgat ggagcaccat tgattgataa aacaattgga gaactgtctt
     6301 cttcaaacga ggagccttta tacagagctg tggcagtcgc ccgagaaggt caaactatta
     6361 ttccaagacg tgacacaaaa ttcaaagaac atgatttggt atttgtaatc tccaaacggg
     6421 caggtgttca cgaagtcatg gcctattccg gaaaaaataa cattaatgtc agaagattaa
     6481 tgattgttgg aggtggcaga attggagaga tggttgcaaa aaagcttgaa aacagtgtag
     6541 attatataaa attaatcgaa ttacgaaagg aaagatgcga attacttaac gaaaaattat
     6601 ccaaaacgct ggtaataaat ggagatgcaa gaaacaccga tctgttgatt gaagaagacg
     6661 taaacgaatt tgacgcattt gtagctgtca ccagcagctc tgaaacaaat atactttctt
     6721 gtgtcatggc aaagaagatg ggtgtggcta aaaccatcgc cgaagttgag aatatagagt
     6781 atataaagtt ggcagaaaat atgggcgtag atgctgtcat caataaaaaa ctaatcacag
     6841 ctagtagaat tttcaggttt acactttcca ataaagtgca gtctataaaa tgtcttaatg
     6901 gctcagatgc agaaattctt gaatttattg tcaatcccaa cagtttaata actaaggata
     6961 agattcggaa tttagggttc cctaaagatg ccatcattgg aggagttata agaggtaatc
     7021 acagctatat agcacttgga gacactgaaa ttaaggcata tgacagagtt gtggtatttg
     7081 ctctgccatc tgcactcaaa aaggtcaaca agttttttat atagcctgct gttctttatg
     7141 gcgaccattc ttcaagctga gaatatttcc aagtcctatg gcacccggat tctattcaat
     7201 aatctggaaa taaacataaa tgaaggtgac agaattgcac ttattgctcc caatggaagt
     7261 ggtaaaagta cactcctccg tatccttgcc ggaaaagata gctcagatat tggcggatct
     7321 gttcgttgca gagagagtgt taaaatagca tggcttgagc aagaccccag ccttaatcct
     7381 gaaagagatg tatttgaaga ggtatttgct tcatctgatg agctttccaa gacagtagaa
     7441 gaatatgaaa gagcgattct ttcaaataac actgacataa ttggctcagc aattcgcgaa
     7501 atggacagat taaatggatg gcaatacgaa ttaaaggtca aacaaatact ctcattactc
     7561 aagatagaca acctcaatca aaaagttgca accctctcgg gaggtcagcg taaaagagta
     7621 gcaattgcag gaatcatgct cgccaatgca gatcttcttg ttctggacga acctacaaat
     7681 catctcgacc ttgaagtcat tgaatatttg gaagaatatt tatgtaaacc atctctctcc
     7741 ttactaatgg ttactcatga ccgatatttc ctcgacagag tatgtactca aattatggaa
     7801 cttgacaacg gtcaattata ccgataccag ggtaattact cctactacct gcaaaaaaaa
     7861 gaagaacgaa ttgaaaattt caactctgaa actgacagag caaggaatct cttcagaaaa
     7921 gagcttgaat ggataagaag aatgccccag gcaagagcca ccaaagctaa atacagaatt
     7981 aatgcattcg aacagatcaa agaaagagca ttacaaaaaa aagttaacca caaaatagca
     8041 attgatgtta aaagtgaaag acttggctca aagattatta attgcaaggg agtaactcat
     8101 cattttggcg attactgtac aatcgacaac ttttcctaca attttgcaaa gggagaaaag
     8161 gtgggattag tgggcccaaa tggtgtagga aagacaactt ttctcaatat tatcagtgaa
     8221 ctgataccac cgcaaagtgg aatcatcgaa accggagaga cagtggtaaa gggttattac
     8281 aaacaaagcg gcctcgaata tactcctgaa gaaactgtaa tagatgttgt tagaaaatat
     8341 tcggaaattg ctaccctttc tgatggcagc attatacctg tcacgcaatt cctttcaaaa
     8401 tttctttttc catattcctg tcacaacact ccagtaagca ggctaagtgg tggagaaaga
     8461 agaagactct atcttgtaac aatattgatg aaaaatccca attttctcat tcttgacgaa
     8521 cctacaaacg accttgacat aataacattg aatgttcttg aagattatct ttctgacttc
     8581 aaaggatgtt tgataattgt atctcatgac cgtttttttc tcgataagct tgcagatcat
     8641 ctttttgtat tcatgggaaa tgggataatc aaggatttcc cgggcaatta ttctgactat
     8701 aaagaatatt gccgtaattt ggaaaaagaa aaaaatacaa cttccaaaaa gattgaagaa
     8761 agaaaacaac cttcgctaaa gaaggcaaac aataaatatt cctacaaaga gaagtttgaa
     8821 attacaacac tcgaaactga aattaacttg ctcgaaaaag agaaagcaga aattgaagaa
     8881 atactctcta caggtcatct ttcccaaaaa gaattaacag aaaaatcaaa aagaatagga
     8941 gaaattattt cactcattga ccaaaaaagc gacaggtgga tagagttaaa cagctaaaaa
     9001 taattccatt aacctatcaa tttcttgtat ttaaatcttt tcggttcaga atcacccaga
     9061 cgtctgcgct tgttctcctc atattcagaa taacttcctt caaagaaata gacattggca
     9121 tcgccctcaa aagcaagaat gtgtgtagct attctgtcaa gaaaccatct gtcgtgtgaa
     9181 ataacaactg cacatcccgc aaaattttcc agaccatctt caagagcacg gattgtattg
     9241 acatccacat catttgtcgg ttcgtcaagt agaagaacat tggcctcttc cttcagtgtc
     9301 aaagcaagat gaagtctatt tctctcacca cctgacaaaa tcccacattt tttctcctga
     9361 tcagcacccg agaaattgaa tctcgaaaca taagctcttg catttacttc tctgttacca
     9421 agttttataa actcggaatt ttcagctata gtctgatata cagttttatc aggatctatc
     9481 tgtttgtgct gctggtcaac ataggccaac ttgactgttt caccgattgt aaagcagccc
     9541 ttgtctggct tttcgtaccc cattatcatc ctgaaaagag ttgtttttcc ggctccgttt
     9601 ggcccaataa cgccaactat agcagcagga ggaagcttaa agttcagatt ttcaaaaagt
     9661 agtctctccc caaatgactt ggaaacatcc aaagcttcaa taaccttatc gccgagacgt
     9721 ggcccgtttg gtataaaaat ttccagtttt tcatccttac ttttaccgtc ctcattcaac
     9781 attttttcat attgagaaag tcgggcttta gacttagctt gacgagcttt aggcgacatg
     9841 cgtacccatt ccagttctcg ctcaagggtt ttccgtcgtt tactttcctg tttttcctcc
     9901 atagcaagtc gaacactttt ttgctcaagc caggacgagt aattcccttt ccaaggtata
     9961 ccctctcctc tgtcaagttc caaaatccat cctgcaacat tgtcgagaaa ataacgatca
    10021 tgggtaacag caataactgt ccctctgtac tgttgaagat gcgcttccaa ccattgaata
    10081 ctttcagcat caagatgatt ggtcggttca tccaataata atacatcagg ctctctcaaa
    10141 agtaaacgac aaagtgcaac tctccttctt tcacccccac ttagcgtttt aactgatgcc
    10201 attggttcag gacattggag agcatccata gccctttcaa gtttggaatc aatgtcccaa
    10261 ccattaattt gatctatttt gtcagtcaat tccccctgat attctatcag acgattcatc
    10321 tcttcctcat ccattggttc tgcaaacctg ttgttgacag cctcatattc tttcatcaac
    10381 tccacaacct ccttaacacc ttcctgcact atctcaatta cagtcttgtt atcgtctagt
    10441 ttcggttctt gttcaagata tcccacagaa tatcccggag cccaaactac ttctccttga
    10501 aaatcatttt caattcctgc tattattttc aaaagagtag atttaccgga accattcaac
    10561 ccgattattc caattttagc accataaaaa aaggagaggt aaatatcctt aagaatgact
    10621 ttattgctat ttggcaatat tttgccaaca ccattcatgg aaaaaataat cttttcttca
    10681 gccattttat ctattttgag caaagataat atgacattac atctttccca agttgttgac
    10741 taaagtatcc acatattgcg cagatattat tgaatctatc tgatgtttcc tctgagctat
    10801 agtcgctctg aaaagctcca tatgtgcctt gtcaacactt ttagcaggag gggattccat
    10861 attaagtgga tttacaggct tgccgttttt ccaaaccctg aaatcgagat gtggcccggt
    10921 tgagagaccc gtactaccta catagcctat cacttgccct tgacgtactc taactccctt
    10981 tgctatacca gcaccatatt ttgaaagatg catataagca gtagtaaatg tggcattatg
    11041 ctttatttta acagtattgc caccgccacc tgaatatccc ttttgaataa ctactccgtc
    11101 tcctattgac ataaccggtg tgccttttgg tgctgcataa tcgacacctg tatgtggtct
    11161 aacaatacga gttattgggt gtctgcgcgc ataggtaaat ccagaagaga ctctggtata
    11221 gctcaaaggt gccttaagaa atgctttttt taagttctca cccgaggaat taaaatattg
    11281 tattgcattt gtatcaccct cctggaaaag atatgcgtca taaaccttac ccttactcgt
    11341 aaaggtcgat gcataaaccg tcccaatacc tactacgttg ttctttatca gtatctcatc
    11401 ataaagtacc ttaaaacagt cgcctttttg cagtccgaag aaatctacag accaagcgaa
    11461 aatattttcc agtctggcag ctatttcaaa actcaaacct gctctttgta catccgccca
    11521 taaagaattt tctatcaaaa tatctgcaac tcgaagacgc gtttcagttt gctcattaaa
    11581 gctttttaca aatattgaat cattcagacc aaaaacaaaa tatgaagtag gagtctcttc
    11641 ataaacaagg tatgcaagtc taggtacttc atctcttgta aaatatgctc tatagttttt
    11701 acctactttc aatttcttaa gatcaaacac tcctttgctt gcttcagcaa gagcataagc
    11761 atcagtattt gaaactccaa gatcgctcat tagttttgaa aaaaattgcc ctttttttac
    11821 actcccctcc tgaacatcaa acatggaaac aggaatgcca aattctgtaa taacctcccc
    11881 tggagactct tcatcttttg agttttcctt accgccttca gaacatgaag taacagtagt
    11941 atttattaaa aataaggcaa taaaaaaaat agtaacaggt aaaaaatatg gtagttgttt
    12001 catacgtaaa tattttataa tttattaccc ataaacaggg tatttttcag tg
//
LOCUS       DAOTQL010000039        11931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4916_length_11931_cov_5.007915, whole genome shotgun sequence.
ACCESSION   DAOTQL010000039 DAOTQL010000000
VERSION     DAOTQL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4916_length_11931_cov_5.007915"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="P5293_03720"
     CDS             complement(<1..204)
                     /locus_tag="P5293_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49042.1"
                     /translation="MKERFLFGYDPFVLPFTLGMVFIILYLLVGTARIIISLTYEERI
                     SLLKSLKPKQLFKTIKDVIVDCLL"
     gene            complement(201..536)
                     /locus_tag="P5293_03725"
     CDS             complement(201..536)
                     /locus_tag="P5293_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR49043.1"
                     /translation="MNNFGYSISPSSRIDLDKRDNDKFTRIVLLESGFLKCMACGLCS
                     STCSGGNFTDVSLRRAIEMIQDGQGKNAVVLLKGCMLCGKCNIICPRGINTRNLIRNI
                     LKVYMEELK"
     gene            complement(543..1547)
                     /locus_tag="P5293_03730"
     CDS             complement(543..1547)
                     /locus_tag="P5293_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR49044.1"
                     /translation="MNKEVLIIGGGPAGLECANTLLMLGYKVFIVEKDSELGGHLRKW
                     DRLFPESISASETLERLLIPSKKAVVFNETEVSSVKLTDGFFDVTLSDNTELHVHSVV
                     VATGFKLFPAEKKEEYGYGIYNRVITNADLEASFKNHSRDILQSKRVGFVHCVGSRDE
                     KISNRHCSKVCCVTAVKQACEFKEANPDAQIYCFYMDLRMFGRGYEDLYLKAQNKYGI
                     RFIRGRVSEVAENVDGELVIKAEDTLISRPLKVTLDLLVLMSGMSKNPDNDKLADMLG
                     LDIAEDGFFVQHDPTINSIKSKIKGIFFAGTVTGPKTIPETLNEARSAALAVHKYLSE
                     "
     gene            complement(1547..2563)
                     /locus_tag="P5293_03735"
     CDS             complement(1547..2563)
                     /locus_tag="P5293_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRR49045.1"
                     /translation="MIINGKKKIWSDYQKEIPDDHYFYVRSCIRQNFFPASEKLFLEI
                     VRDRLNLDFYETEHHTTCGGIAYHSDTIPQETVMTIVARQFALMSEAGYKNYVASCIT
                     SFGLYTEILDTWYHYPELEAKIKENLWKTTRREFNKPEYLAHASDVIYKLRDKIASQA
                     RYKLVNKDTGKPLKVVEHIGCHYSKMFPSKGVGGAEYPYVLTGMIESWGGEIVDYPER
                     RHCCGFGFRQYMVRANRGYSLSNTYKKFESMEPFHPDMIITNCPGCPYFLDRWQYVIK
                     EMEGKTYGKEGQGIPVFTYEEVAGLVLGYDPWYLGLQMHQIDVEPLLDKIGIKYSKTD
                     KYLL"
     gene            complement(2560..3252)
                     /locus_tag="P5293_03740"
     CDS             complement(2560..3252)
                     /locus_tag="P5293_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR49046.1"
                     /translation="MGKLLDKLNKDYRFLEGLNACINCGTCTAICPAAEFYKYDPRII
                     TDTVQSKNEDEIEELLKSDTIWYCGECMSCVTRCPRKNAPGLIIMALRTLSMDTGLFI
                     NSEKGRQQYALSLALNNNILNYGYCVYPRTFKYEDHPEYGPVGKWIENNLDAVHKKVG
                     SNLDGQGPGILRKIPDEDLQELKRIFDLTGATERMNTIEKYSLSKAQELGMTKEEYLN
                     YIETTIKTEHHK"
     gene            complement(3237..3695)
                     /locus_tag="P5293_03745"
     CDS             complement(3237..3695)
                     /locus_tag="P5293_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRR49047.1"
                     /translation="MILNKKVGVFLNLVHCRFKLYLSTIFQDNGFDITPEQFLVLDTL
                     WDDGVQSQQEIADKIQKDKNSVTKLIDALEKKNYVKRSQDKQDRRLNLINITDEGLKI
                     KDAVTKIAMEATNIIIRDIPLEDLYTFIRVAEKMLDNMVIKKNQIKWENY"
     gene            3774..5030
                     /gene="rmuC"
                     /locus_tag="P5293_03750"
     CDS             3774..5030
                     /gene="rmuC"
                     /locus_tag="P5293_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRR49048.1"
                     /translation="MALLLLVIITILVIILLVRSESLLSLRNSIEKLENTLSDEIRYL
                     REENSRSLRENRDELNNALKIFSTSIGDGMKNFQDASERGMNLLSDIQKEKLSQIENR
                     QNEMIKNTEEKLEKIRSVVEEKLEKTLSERLGQSFETVGKQLIEVQKGLGEMQALAQD
                     VGGLKKVLSNVKMRGGIGEVQLSMLLEQILAPDQYEPNVKTKHDGKEFVEFAIKLPGK
                     DDLIQNIWLPVDAKFPSEAYEQLQDAYETNDLLRIESEQKNLENVIKKMAKDIFEKYI
                     DPPYTTDFAIMFLPFEGIYAEVVRKASLLEEVQRKFKVIVTGPTTLAAILNSLQMGFR
                     TLAIEKRSSEVWRILGAVKTEFDTFGGLLQKAQKNIQGGLDDIDKLVGTRTNVIRRKL
                     KEVEALDEAISKEILGEGDKPQSDDL"
     gene            complement(5025..8249)
                     /gene="carB"
                     /locus_tag="P5293_03755"
     CDS             complement(5025..8249)
                     /gene="carB"
                     /locus_tag="P5293_03755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HRR49049.1"
                     /translation="MERNLNKVLLLGSGALKIGEAGEFDYSGSQALKALKEEGIATVL
                     INPNIATVQTSEGVADKIYFLPVTPYFVEEVIKKEKPDGILLAFGGQTALNCGVQLHK
                     SGVFDRYNLKVLGTPVQAIIDTEDRELFKERLEEISVKTINSQAVENLDDAVTAAKIL
                     GYPVILRAAYALGGMGSGFCDNEEELVILAKKAFSYSPQVLIEKSLKGWKEIEYEVVR
                     DRYDNCITVCNMENFDPLGIHTGESIVVAPSQTLTNREYHLLRELSIRIVRHIGIIGE
                     CNVQYAFDPKSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFELKNSV
                     TKSTPAFFEPALDYIVCKIPRWDLSKFQGVSREIGSSMKSVGEVMAIGRSFEEAIQKG
                     LRMIGQGAHGFVANREIKVENIEHSLSAPTDNRIFVISKAFNEGYTVDQIHEYTKIDK
                     WFIDKLYNIFLTSQQISEVDNMEDLNIDLLRKAKKEGFSDFQIGRLIFKDKIDVDEAS
                     ARIRQFRKQNGIVPVVKQIDTLAAEYPAQTNYLYLTYNGTYNDVRYFGDHKSIIVLGS
                     GAYRIGSSVEFDWCSVNALQTIRNRGWRGVMINYNPETVSTDYDMCDRLYFDELTFER
                     VMDIYELENPYGIIVSVGGQIPNNLALKLDRSGVKLLGTSAESIDNAEDRHKFSSMLD
                     RLQIDQPRWKELSTLDEVKEFVDEVGYPVLVRPSYVLSGAAMNVCSNNNELEQFLQLA
                     ANVSKRHPVVVSEFIQNAKEIECDAVADKGNVIAYAISEHIEFAGVHSGDATIQFPPQ
                     KLYVETFRRIKKIAKKIAEELKISGPFNIQFLAKENDIKVIECNLRASRSFPFVSKVI
                     KINFIDLATRIMIGEEVLPPHKSAFDLDYVGIKASQFSFSRLQKADPVLGVDMASTGE
                     VGCLGDDTNEAILKAMISVGYRVPEKNILISSGDPKQKADLLSVAKLLADKGYNLFAT
                     GGTYKFLTDSHVNATHVYWPSETNKEPQALKMIQNKQVELVINIPKNLSQIELENGYK
                     IRRAAVDYNIPLITNARLAAAFINAFCTLSLEDIRIKSWDEYIS"
     gene            complement(8252..9322)
                     /gene="carA"
                     /locus_tag="P5293_03760"
     CDS             complement(8252..9322)
                     /gene="carA"
                     /locus_tag="P5293_03760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRR49050.1"
                     /translation="MGDNRVAKLYLESGVEFVGYSFGNDQAIDGEVVFSTAMVGYPES
                     LTDPSYSGQILCVTFPLVGNYGVPEDTLINNLSTLFESEKIHVRGLIISDYSFNYSHW
                     NAAKSLDKWLKENNITGIYGVDTRELTKILREEGSLLGKIIPEGTSLEGYYDPNTVNQ
                     VDIVSCKEVIHYGSGYKKIVLVDCGVKHNIIRCLLRRGVEIIRVPWNFNFHTIDYDGL
                     FISNGPGNPEFCTTAIDNIARAFEMEKPIFGICMGNQLMAKAGGARTYKMKYGHRSHN
                     QPVRLVNSDRCFITSQNHGFAVDNSTLGTNWEPLFINMNDNSNEGIKHKYKPFFSCQF
                     HPEASSGPTDTEFLFDDFIKQL"
     gene            complement(9392..10969)
                     /locus_tag="P5293_03765"
     CDS             complement(9392..10969)
                     /locus_tag="P5293_03765"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRR49051.1"
                     /translation="MKKTYLSLLTLIVSSSLSFAQTNLYKESCTSIMVGKGASTDGSV
                     MTAHTCDANYRTWVEITPSKSFKPGDMEPIYWGTLHNEEPWDMRNVKEKGKIPAPEKT
                     FAYLNTAYPCLNEKQLAIGETTIEGRPELLNENGLFPIEELERIALQRCSTAVDAIKL
                     IGRLAEQYGYGDWGECITIADKKEVWQMEIFGSGPGKPSAIWVAQRIPDDHIGISANI
                     PRISKVNFNDQRNFMYSSDIKERVIKLGLWDGKGDFVFWKINSGKKPFSVREYFVFNT
                     LAPSLNLQFDAEELPFSIKPDKKVDVRDILAMYRQTYDGTQFDQTANLFVVVPRKDAV
                     RGEYQDTVRPVSTFMTNDMRALLNSLKPGVTERNRTIAVIQCSYSHVIQLRDWLPDEV
                     GGVAYFAFDNPAQSPKIPIYSGTTTLPKSYSICGQHRYREDAAIWTFRETNRIATINW
                     DKTRKIIEPQLMSLEDAMFRDAANIETAATKLINNGKPEEAKKMLTEFTNNFAAAAMR
                     RWSELKAELWIIFARSM"
     gene            complement(11017..>11931)
                     /locus_tag="P5293_03770"
     CDS             complement(11017..>11931)
                     /locus_tag="P5293_03770"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRR49052.1"
                     /translation="DKFGDTPIGMAKAAMEWVKMAQINDFDQIVISLKSSNTRVMVDA
                     YRMLYHEMAKTGIIYPIHLGVTEAGNGEPGRIKSAVGISTLLSEGIGDTIRVSLTEDP
                     VNELPVAKLIASFFEKGHYYSPVFNTFIRDDGTVVCHRSYKCNDWNDFIITASCELGP
                     LLLDKKIDDISIELEMQSPYTKNDIDRFRRELLQASRRVFFEPEYVACPGCGRTLFNI
                     ESVFSEVKKRTAHLKGYVIAVMGCIVNGPGEMADADYGYVGEGKNKVSIYRGKDPIFR
                     AVPEEIAIDKLLELIESDAKSGIFMPHF"
BASE COUNT         3628 a         2355 c         2004 g         3944 t
ORIGIN      
        1 caacaggcaa tctacaatga catctttaat ggttttaaaa agttgcttgg gttttagaga
       61 ttttagtaga gagattcgtt cctcataagt gagggatata ataattctgg ctgttcctac
      121 aagaagatac agaataataa aaaccattcc aagcgtaaag gggagtacaa aagggtcata
      181 gccaaataaa aacctttcct tcatttcagt tcctccatgt aaactttaag aatgtttctt
      241 attaaatttc tcgtgtttat gcctcttgga cagataatat tacattttcc acaaagcatg
      301 catcccttga gcaacactac agcatttttt ccctgcccat cttgaatcat ttcaatagct
      361 cttctaagac tcacatctgt aaaattacct ccactgcatg tagaagaaca caatccacaa
      421 gccatacatt taaggaatcc ggattctaat aagacaattc ttgtaaattt gtcattatct
      481 cgtttgtcaa ggtctattct tgaacttgga gatatcgaat atccaaaatt attcatacca
      541 atttattcag atagatattt atgcacagcc aaagctgccg atcgagcttc atttaatgtt
      601 tcgggaattg ttttaggacc tgttaccgtt ccggcaaaaa atattccttt tattttgctt
      661 tttatactgt ttatagtagg atcatgctga acaaaaaaac catcttcggc tatatctaac
      721 ccaagcatat cggctaattt atcattatcc ggatttttcg acatgccact catcaaaaca
      781 agcaaatcga gagtgacttt taatggtctg gatattaaag tatcttcagc ctttattact
      841 aattctccat ctacattctc agcaacttct gaaactctgc ctctgatgaa ccggatacca
      901 tatttatttt gggctttgag atacagatct tcataacctc tgccaaacat tcttaaatcc
      961 atgtaaaaac aatagatttg agcatcggga tttgcttcct taaattcaca tgcctgcttg
     1021 acagctgtca cacagcaaac tttggaacaa tgcctgttgg aaattttttc atctcttgac
     1081 ccaacacagt gtacaaatcc tactcttttt gattgtaata tatctcttga atgattttta
     1141 aagcttgctt ctaaatcagc atttgtaatg actctgttat atataccata tccatattct
     1201 tctttttttt ctgcagggaa aagtttaaaa cctgtagcca caacaaccga atgtacatga
     1261 agctcagtat tgtcggaaag cgtaacatca aaaaaaccat ctgtcaattt aacagaactt
     1321 acttcagttt cattaaatac aactgctttt ttggatggaa taagaagtct ttcaagagtt
     1381 tcagaggctg atatactttc ggggaaaagc ctgtcccatt ttctcaaatg tcctccaagt
     1441 tcagagtctt tttcaacaat gaaaacttta taaccaagca taagaagtgt attggcacac
     1501 tccaatccgg caggtccacc gccgataatt aaaacctcct tattcatcac aatagatatt
     1561 tatctgtttt actatatttg attccaatct tgtctaagag aggttctaca tcaatttgat
     1621 gcatctgcaa cccaagatac catggatcat atccgagtac caatcccgct acttcttcat
     1681 aagtaaatac cggaattccc tgaccttcct ttccataagt tttgccttcc atctccttaa
     1741 taacatattg ccatctgtca agaaaatatg gacacccggg acagttagtg attatcatat
     1801 cggggtgaaa cggctccatt gattcaaatt ttttgtatgt gtttgataaa gagtatccac
     1861 gattagctct gaccatgtat tgtctgaatc caaagccaca gcagtgtctt ctttccggat
     1921 aatcgacaat ttcacctccc catgactcga tcattcctgt aaggacataa ggatattcag
     1981 caccaccgac accttttgag ggaaacattt tagaatagtg acatccaatg tgttccacaa
     2041 ctttcagcgg ctttcccgta tctttgttta caagtttata cctggcctgc gaagcaattt
     2101 tgtctctaag cttatatatc acgtcgcttg cgtgggcaag atattccggt ttattaaact
     2161 ctcttcttgt agttttccaa agattctctt tgattttagc ttctaattcc ggataatgat
     2221 accaggtatc gagaatttct gtatacaagc cgaaagaagt tatacaagat gcaacatagt
     2281 ttttataacc tgcttcactc atcagtgcaa actgccttgc aacaatcgtc atgactgtct
     2341 cttgagggat agtatcactg tgataagcta tgcctccaca agttgtatgg tgttccgtct
     2401 cataaaaatc caaatttaga cgatctctga ctatctctaa aaaaagtttt tcggaggcag
     2461 gaaaaaaatt ctgtcgaatg cagcttctga cataaaaata gtggtcatca ggaatctcct
     2521 tttgataatc actccaaatt ttctttttcc cgtttattat catttatgat gttctgtttt
     2581 aatggtagtt tcaatataat tcagatattc ttctttagtc attccaagct cctgagcttt
     2641 agaaagagaa tacttttcaa ttgtattcat cctctctgta gctcctgtca agtcaaaaat
     2701 tctcttaagc tcctgcagat cttcatcagg tattttgcgt agaatgccag gtccttgacc
     2761 atcaagatta gaaccaacct ttttatgtac agcatccaaa ttgttttcta tccatttacc
     2821 tacgggacca tattcaggat gatcctcata cttgaaagtt cttggataaa cacaataacc
     2881 gtaatttaat atattgttgt taagtgctaa agatagggca tactgttgtc ttcccttttc
     2941 ggaattaata aacaatcctg tatccataga gagtgtcctg agagccatga ttattagtcc
     3001 tggagcattc tttcttggac atcttgtcac acaggacata cattctccac aataccatat
     3061 agtatcgctc ttcaagagtt cttctatctc gtcttcattt ttagactgaa ccgtgtctgt
     3121 tataattctt ggatcatatt tgtaaaattc tgctgcagga catatcgcag tgcaagtacc
     3181 gcagttgata caggcattca acccttccaa aaaacgataa tctttattta acttgtctag
     3241 taattttccc attttatctg atttttctta ataaccatgt tatcgagcat tttttctgca
     3301 actcgaataa atgtgtaaag atcttcgaga ggaatatccc ttataattat gtttgttgcc
     3361 tccatggcta ttttggtaac agcatccttt atcttcagcc cttcatcagt aatgtttata
     3421 aggttgagcc ttctatcctg tttgtcctgt gaccttttaa cataattctt tttttcaaga
     3481 gcatctataa gctttgtaac agaatttttg tctttttgga ttttatctgc aatttcctgc
     3541 tgagactgaa caccgtcatc ccaaagtgta tctagaacta aaaactgttc aggagtgatg
     3601 tcaaatccat tatcctggaa tatggtactg agatatagtt taaatctgca gtgcaccaaa
     3661 ttaaggaaaa ctccaacttt tttatttagt atcatatact atagtataag agtttactat
     3721 tgcaaatata acagaaattt gattaatttg tctatatgaa catatttatt acaattgcac
     3781 ttttattatt ggttattatt accattctgg tgattatttt gcttgtcaga agtgaatcac
     3841 tcctttctct tagaaatagc attgaaaagt tggaaaatac tctatctgat gaaattagat
     3901 atttaaggga agagaattcg cgttcactca gagagaacag agatgagctg aacaacgctt
     3961 taaaaatatt ttctacatct attggagatg gcatgaaaaa ctttcaggat gcttctgaaa
     4021 ggggtatgaa cttgctttct gatattcaaa aagagaaact ttctcagatt gaaaatcgtc
     4081 agaacgaaat gattaagaat actgaagaga aacttgaaaa gataagaagt gtcgttgagg
     4141 aaaagcttga aaaaacttta agtgaaagac ttggacagag ttttgaaact gttggaaagc
     4201 agctcataga agttcagaaa gggctgggag agatgcaggc acttgcacag gacgttggag
     4261 gccttaaaaa agttttgagc aacgtaaaaa tgagaggcgg gataggagaa gttcagctat
     4321 caatgcttct tgagcagata cttgctcctg accagtatga acctaatgtc aagacaaagc
     4381 acgatggaaa agagtttgta gaattcgcca ttaaattacc aggaaaagat gatctgattc
     4441 aaaatatatg gttgcctgtg gatgcaaagt tcccaagtga agcatatgag caactgcaag
     4501 atgcctatga aacaaatgat cttttacgca ttgaatcaga acagaaaaat cttgagaatg
     4561 ttataaaaaa gatggccaag gatatctttg aaaagtatat tgatcctcca tacactacag
     4621 attttgcaat aatgttttta ccttttgagg gtatttatgc agaggtagtt agaaaagcct
     4681 cgttactgga agaagtacag agaaagttca aggttattgt gactggcccc actactttag
     4741 cagctatatt aaacagtctg cagatgggat tcaggacact tgctattgag aagagaagta
     4801 gtgaagtatg gagaattctt ggagcagtta agaccgagtt tgacacattt ggcggtcttc
     4861 ttcaaaaagc gcagaaaaat attcagggag ggctcgatga tatcgacaaa ctggtgggaa
     4921 ccagaactaa tgtcataaga cgcaaattaa aagaagtcga agctcttgac gaagctatct
     4981 caaaagagat cttgggagag ggggataaac ctcaatctga tgacttatga tatatattca
     5041 tcccagcttt tgatacgtat atcctccaga gagagtgtgc agaatgcatt gataaatgct
     5101 gcagcaagtc ttgcatttgt tattagtggt atattgtagt caacagcagc tcttcttatc
     5161 ttatatccat tttccagttc aatttgtgat aaatttttag ggatatttat aacaagctca
     5221 acctgtttgt tttgaatcat tttaagagct tgaggctctt tattggtttc gctcggccaa
     5281 taaacgtgtg tggcattgac atgactgtca gtaaggaact tatatgttcc gcctgtagca
     5341 aataaattat atcccttatc agccaataat tttgcgacag ataataagtc tgccttttgt
     5401 ttaggatctc ccgatgagat gagaatattt ttttctggca ctctgtaacc tactgaaatc
     5461 atggccttaa ggatagcttc attagtgtca tcacccagac agccaacttc tcctgttgag
     5521 gccatatcaa ctcctagcac cggatcagct ttctgaagtc gtgaaaaaga gaattgcgat
     5581 gctttaatgc cgacataatc caagtcaaag gcacttttgt gtggtggtag aacttcttct
     5641 ccgatcataa tcctggtagc taaatcaata aagttgattt taataacctt tgatacaaat
     5701 ggaaaacttc ttgacgctct tagattgcac tcgattacct taatatcatt ctcttttgcc
     5761 agaaactgaa tattgaaagg tcccgaaatt ttaagctctt ctgcaatttt ctttgcaatt
     5821 ttttttattc ttctaaaagt ctcgacatat agtttttgtg gaggaaactg tattgttgca
     5881 tctcccgaat gtacgccggc aaattctata tgttcggata tagcataggc tattacattc
     5941 cctttatcgg caacagcatc acattcaatt tccttggcat tttggataaa ctctgaaact
     6001 acaacaggat gtcttttaga aacatttgca gcaagttgca gaaattgttc gagttcattg
     6061 ttgtttgaac aaacattcat agctgcgccg gatagaacgt aagatggacg aacaaggact
     6121 ggatatccga cttcatctac aaactccttt acctcatcaa gagttgaaag ctctttccat
     6181 cttggttgat caatctgaag ccggtcaagc attgaagaaa atttatgtct atcttcagca
     6241 ttatctatac tttcagcact tgttcccaat aatttaacac cggacctgtc aagtttcaga
     6301 gccaggttat ttggtatctg tcccccaacg gatacaatta ttccataagg attctcaagc
     6361 tcataaatgt ccataaccct ttcaaaagta agttcgtcaa agtaaagacg gtcgcacata
     6421 tcataatcag tagaaacagt ttcaggatta taattaatca tgacacctcg ccagcctctg
     6481 tttcggattg tttgaagggc attgacactg caccagtcga attcaactga gcttcctata
     6541 cggtatgctc cagaaccaag aactataata gacttatgat caccaaaata tctgacatca
     6601 ttataagttc cgttataagt gagatacaga taattggtct gtgcaggata ttcggctgct
     6661 agggtatcta tttgtttgac aacaggtaca ataccattct gcttgcgaaa ttgtcttatg
     6721 cgggcagatg cttcatcaac gtcaatttta tccttgaaga tcaatctgcc aatttggaaa
     6781 tcagaaaatc cctctttttt tgcttttctt aaaagatcaa tattaagatc ttccatattg
     6841 tcaacttctg agatttgctg tgatgtcaaa aagatattat aaagcttatc tataaaccat
     6901 ttgtcaatct tagtatattc atgaatctga tcaactgtgt agccctcatt aaaagctttg
     6961 gagatgacaa aaattctatt atctgttggt gcgctgaggg aatgttcaat attttcaact
     7021 ttaatttctc tgtttgctac aaagccgtga gctccctgac ctatcatcct gaggcctttt
     7081 tgaatggctt cttcaaaact ccttcctatt gccataacct caccaacact tttcatactt
     7141 gagccaatct ctctcgatac tccttgaaat ttggagagat cccatcttgg gattttgcaa
     7201 acaatataat caagagcagg ttcaaagaat gcaggagtac ttttggttac agagtttttc
     7261 aattcaaaaa ggccatatcc aagaccaagt tttgcagcta caaatgcaag aggataacca
     7321 gttgctttag aagctaaagc agacgatctg ctcaatcttg cattgacctc aattacccta
     7381 taatcttctg atttaggatc aaaagcatac tgaacattac attcacctat aataccaatg
     7441 tgtctaacga ttcttatgga taattctctt aaaagatgat attctcggtt tgtaagagtc
     7501 tgagatggcg ccacaacaat actttcacct gtgtgaatcc caagagggtc aaagttttcc
     7561 atattacaga cagttataca gttatcatat ctgtctctca caacctcata ttcaatttcc
     7621 ttccagcctt tgagagactt ttctattaaa acttgaggag aatatgaaaa tgcctttttt
     7681 gcaagtataa ccagttcttc ttcattgtca caaaaaccgc ttcccatccc acctaatgca
     7741 taggcagctc ttaatattac aggatatcct aaaatttttg ctgcagtaac tgcatcatca
     7801 agattttcta cagcttgact attaattgtt ttgacagata tctcttccaa tctttcttta
     7861 aacaactctc tgtcttcagt atctattatt gcttgaacag gtgtcccaag aacttttaga
     7921 ttatatctgt caaaaacacc ggatttgtgg agttgcacac cgcaatttaa agctgtttgt
     7981 cctccgaatg caagtaaaat cccatcaggt ttctctttct ttatcacttc ttcaacaaaa
     8041 taaggagtta ccggtaaaaa atatatttta tcagctactc cttcagatgt ttgaaccgtt
     8101 gctatatttg gatttataag gactgttgca ataccttctt cctttaaagc tttaagggcc
     8161 tgagaacctg aatagtcaaa ttctccagct tctcctattt taagagctcc agaaccaaga
     8221 agaagtactt tattaagatt tctttccatt attacagttg tttaataaag tcatcaaata
     8281 ggaattcagt atctgtagga ccacttgagg cctcaggatg aaattgacac gaaaaaaatg
     8341 gtttatattt atgctttatc ccttcatttg aattatcgtt catattaata aagagaggtt
     8401 cccaatttgt gccgagcgtt gaattatcaa cggcaaatcc gtggttttga gatgttatga
     8461 aacatcggtc agaattaacc aatcttacag gttgattgtg gctcctatga ccatatttca
     8521 ttttgtatgt tctagctcca ccagctttag ccattaattg attccccata caaattccaa
     8581 aaattggctt ttccatttca aatgccctgg cgatattatc aatagctgta gtacaaaact
     8641 caggattacc cggtccattt gaaataaata aaccatcata atcaatagta tgaaagttaa
     8701 aattccatgg aaccctgatt atttcgacac ctcttcttaa aagacacctt atgatattgt
     8761 gctttactcc acaatctaca agaacaattt tcttatagcc cgaaccataa tgaataactt
     8821 ctttgcaact tacaatatca acctgattga cagtgttagg atcatagtag ccttccagag
     8881 aagtgccttc tggaataatt ttaccaagta gcgatccttc ttctcttaaa atttttgtaa
     8941 gttctcttgt atcaactcca taaatgcctg ttatattgtt ctctttaagc catttatcga
     9001 gacttttggc tgcattccag tgactgtaat tgaatgaata gtcagaaata attaatcctc
     9061 ttacatggat tttctcagat tcaaacagtg tagaaagatt gttaattaaa gtatcttccg
     9121 gaacaccgta gtttcctact agaggaaaag taacacacag tatctgtcct gaataggagg
     9181 gatcagttag actttcggga taaccaacca tggctgtaga aaaaacgacc tcaccatcaa
     9241 tggcttggtc gtttccaaat gagtaaccga caaattcaac cccggattcg agatatagct
     9301 ttgcaactct attgtctccc attatttttc cgattaaaat tgttaaaatc gggcaaaggt
     9361 aatgaaaaag ggggagtagt taaaaaatat gctacatgct ccgtgcaaaa attatccaaa
     9421 gttcagcttt gagctctgac catcttctca ttgcagcagc agcaaaattg tttgtgaatt
     9481 ctgtcaacat tttttttgct tcttcaggtt taccgttatt tattaatttt gttgcggctg
     9541 tttcaatatt tgcagcatct ctaaacatcg catcttccaa agacataagc tgaggttcta
     9601 ttatctttcg tgttttatcc cagtttattg ttgctattct gtttgtttct ctgaaagtcc
     9661 aaatagctgc atcttctcta taccgatgtt gtccacaaat agaatagctt ttaggcaatg
     9721 ttgttgtacc agaatatata ggaatttttg gactttgtgc cggattgtcg aaagcaaaat
     9781 aggccacacc tccaacttca tcaggtaacc agtctcttag ttgaatgaca tgagagtagg
     9841 agcattgaat gacggcaata gttctattcc tttctgtaac gccaggcttc aaagaattta
     9901 acaaagctct catatcatta gtcataaagg ttgaaacagg ccttacagta tcttgatact
     9961 ctcctcttac ggcatcttta cgtggcacaa ctacaaaaag atttgctgtc tggtcaaatt
    10021 gtgtaccatc ataagtttgt ctgtacattg ccaagatatc tctgacatca acttttttgt
    10081 caggttttat tgagaatggc aactcttctg catcaaactg aaggtttaaa gatggagcaa
    10141 gagtattgaa aacaaaatat tctctaacag aaaacggttt tttaccacta ttgattttcc
    10201 aaaatacaaa atcgcccttt ccatcccaaa gaccaagttt tataactctt tctttgatgt
    10261 ctgagctgta cataaaattg cgttggtcgt taaaattgac tttagaaatt cttggaatat
    10321 tggctgagat cccaatgtga tcatcaggta ttctttgagc tacccatatt gctgaaggtt
    10381 taccgggacc tgaaccaaaa atttccattt gccaaacctc ttttttgtca gctatagtaa
    10441 tgcactctcc ccaatctcca taaccatact gttctgcaag tctgccaata agtttaatgg
    10501 catctactgc tgtcgaacac ctttgaagag caatcctttc aagttcttca attggaaaaa
    10561 gaccattttc atttaataat tcaggtctgc cttctatagt tgtctctccg attgcgagtt
    10621 gtttttcgtt gagacaagga taagctgtat ttaaataagc aaaagtcttt tctggggctg
    10681 ggattttacc tttttcttta acatttctca tatcccaagg ctcttcattg tgaagagttc
    10741 cccagtaaat aggctccata tctcctggct tgaatgattt tgaaggggtt atttcaaccc
    10801 atgttcgata atttgcatca cacgtatgtg ctgtcataac tgagccatca gtactcgctc
    10861 ctttacctac cattatggag gtacaacttt ctttatacag atttgtctgg gcaaaagaaa
    10921 gagaagagct aacaatcaaa gttagaagag ataaataagt ttttttcata aaattttatt
    10981 tagatatttc aactcaacag actgtaagaa aagtgattag aaatgtggca tgaatattcc
    11041 acttttagca tcagattcaa ttagttcaag aagcttgtct attgctattt cttccgggac
    11101 tgctctgaat attggatcct ttcctctata tatagatacc ttgttctttc cttctccgac
    11161 ataaccataa tcggcatcgg ccatttctcc aggaccattg acaatgcatc ccataacagc
    11221 aataacatat ccctttaaat gagccgttct tttcttaact tctgaaaaaa ctgattcaat
    11281 attaaaaagt gttcttccac atcctggaca agcaacgtat tcaggttcaa aaaatactcg
    11341 tctcgatgcc tgcaatagct ctctgcgaaa tctgtcaatg tcattttttg tataaggcga
    11401 ctgcatttca agttcaatcg atatatcatc aatctttttg tctaaaagta atggtcctaa
    11461 ttcgcaagaa gctgttataa taaagtcgtt ccaatcgttg catttatatg agcgatgaca
    11521 aacgactgtg ccatcgtctc tgataaatgt attaaataca ggagaataat aatgaccttt
    11581 ttcaaagaaa gaagcaatca attttgctac aggtaattca tttacaggat cttcagttaa
    11641 tgaaactcgg atggtatcac cgattccctc agaaagaaga gtagaaattc ctacagccga
    11701 tttaatcctt ccaggctcgc catttcctgc ttctgttact ccaaggtgaa ttggatatat
    11761 tattcctgtt ttcgccattt catggtacaa cattctgtaa gcatctacca taactcttgt
    11821 gttacttgat ttgagcgaaa tgacaatttg gtcaaaatca tttatttgtg ccattttaac
    11881 ccattccatg gctgcttttg ccataccaat aggagtatca ccaaatttat c
//
LOCUS       DAOTQL010000040        11924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4923_length_11924_cov_5.658269, whole genome shotgun sequence.
ACCESSION   DAOTQL010000040 DAOTQL010000000
VERSION     DAOTQL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4923_length_11924_cov_5.658269"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="P5293_03775"
     CDS             <1..326
                     /locus_tag="P5293_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HRR49053.1"
                     /translation="MEPFHPDLIIANCPGCTFFLDRWQYVISEIEGKIYGDNGYGIPV
                     LTYEELAGLLMGYNPWDVGLQLHQVAVEPLLDKLGVKYNPDDKYKGRDGKILKLPQQS
                     LLKMY"
     gene            complement(347..2443)
                     /locus_tag="P5293_03780"
     CDS             complement(347..2443)
                     /locus_tag="P5293_03780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRR49054.1"
                     /translation="MTKLGTVIVLIALIILQSCDRKMTENPLLLTSNNQYGAPAFDKI
                     KTFHFKPAFEAAITASKARIDSIINNPDAPTFANTVEALEFASRNLSNIASIFFALNG
                     SNTDSSMQKVALEVSPMLSDFGNDIMLNEKLFNRIKSVYSAKDSLQLNQEQKRLLEET
                     YKSFTRNGANLNPKQKAELKKIDNELAQLSQKFDQNVLGATNKFFLHITDSSQLVGLP
                     EFVKEMGASEAKSRSLEGWVYTLQYPSYGPFLQYSENRELKEKLWKASNRKAFGDEFD
                     NQEIIKKIVGLRAKRAKLLGFKNHAEYILSDNMAKNPETVNKFLSDLLDKSLPYARKD
                     LANIQAYADANGFKGKLMPWDFSYYSEKYKHEKFSINDELLKPYFKLENVEKAVFSLA
                     DSLYGLKFVQNKSLPVYHPDVKVFEVFDADGKFLSLLYVDYFPREGKNSGAWMTNFRE
                     EYIMKGVEYRPFVTLVFNFTKPTENKPSLLTFYEVTTMLHEFGHALHGMFAKGSYASL
                     TGTNVKRDFVELPSQIMENWATEPDFLLSFAKHYETGEIIPQEMIQKIVDSKNFLSGY
                     NSVRQISFGINDMAWHTVTETPDKGVMEFEREATQKAQLMPLIDSTCTSTSFSHIFSG
                     GYSAGYYSYKWAEVLEADAFSLFKEKGIFNKEVAASFRENILSKGNLIDADVLYRNFR
                     GRDPRPEALLEKLGMK"
     gene            complement(2447..3340)
                     /locus_tag="P5293_03785"
     CDS             complement(2447..3340)
                     /locus_tag="P5293_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49055.1"
                     /translation="MKKLLTVLLITFFAISLSSCVESSKKYKALQAQMDSLSTLTATK
                     SAEFEEVFSTLNEVENGLKSIREAENILVLQSQKGGAEVNASSRDRLMSDVTAIGEAI
                     KNYKKQIEKLKNDSRIQSAQFMKRLNALEAELNAKSELIADLGRQLEEKENQIKVKTQ
                     QISSLEETVETLKNDVSSLSAESSSQKETISSQEAQINTGYYIVGTKADLIAANVMSK
                     GGLFRSAKISYQAEQSAFVKIDIRELREIKLNATKAKVMSLHPTGTYSIEEDEAGMLV
                     LKITDPKAFWEQTKYLVIQIQ"
     gene            complement(3418..3768)
                     /locus_tag="P5293_03790"
     CDS             complement(3418..3768)
                     /locus_tag="P5293_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49056.1"
                     /translation="MGICKECGKELRGRSDKKFCSDMCRNSFHNKKRKTELIRIAEVN
                     KILLRNNRIFNKFISEDEVFIDINQAIDEGVDLTHFTSIQINPSGETCFVCYDTGFVI
                     KSRNMIKITKMSNS"
     gene            3999..4343
                     /gene="ssb"
                     /locus_tag="P5293_03795"
     CDS             3999..4343
                     /gene="ssb"
                     /locus_tag="P5293_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRR49057.1"
                     /translation="MENWVNKVELRGNVGFDPKLISLEDGGSFIRFSLATNETFKNRK
                     GELHEETVWHNIIAWNGKNIPDFSQIKKGSYLHVTGRIKPVQYQTKSGTERHTYEIIA
                     VSIKLLDRESLE"
     gene            4371..5849
                     /gene="cysS"
                     /locus_tag="P5293_03800"
     CDS             4371..5849
                     /gene="cysS"
                     /locus_tag="P5293_03800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRR49058.1"
                     /translation="MDRKMRDFTIYNTLSRKKEKFETIHPQMVGIYVCGPTVYGDAHL
                     GHARPAVTYDVLVRLFRHLGYKVRYVRNITDVGHLESDADEGDDKILKKAKLEQLEPM
                     EIVQRYTNRFHECMRLLNTLPPDIEPRASGHIIEQIALVKTILDNGFAYESRGSVYFD
                     VEKYNKKFKYGVLSGRQLEDLQSNSRSLDGQDDKRSPYDFALWKKASPEHIMRWPSPW
                     GEGFPGWHLECSAMSAKYLGEEFDIHGGGMDLLFPHHECELAQNTASRGVGGVRYWMH
                     NNMITINGQKMGKSLGNFITLEELFEGKHQMLSQPFSPMTVRFFILQAHYRSTLDFSN
                     EALVASERGLKKLIQALKDVVKLPVVTETSELGVDSLIEKIYDSLCDDLNTPVAISHI
                     FDAVKIVNQVKEKQLVLSQSEKEGLVYIFNILLTEILGLKDDKVERSDIEILDEVIKL
                     LLDIRSEAKRKGDFTTSDKIRNNLKALGIEIRDTKEGTEWSY"
     gene            6430..8592
                     /locus_tag="P5293_03805"
     CDS             6430..8592
                     /locus_tag="P5293_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49059.1"
                     /translation="MKFFPTKIVLVALIIVAAVSYASAKSQPRSFAIVVDKNTYENCR
                     ASIDKYSTAVRLDGLNTFVIVDKWNIPDSIRMELKRLYLNNNLEGAVLIGDIPVPMIR
                     DAQHLTTAFKMDQRRPWNLSSVPSDRFYDDFDLKFKFLKRDSTNNLYFYYNLASDSPH
                     HITCDIYSARIKPPTVKGKDKFQLINEYLEKAAREKGIPRSLGAITYFAGHGYNSDCM
                     VARADERYSLTEQFPFVVAGYNRLNYIDHTYDNYVKYRLMAELAREDLGLAILHHHGS
                     EDRQYLNGTPLATTASDWIGLAKKFFRSKIRNSDDTTASKNYYIREYGIPESWVNNAF
                     DPEIVRQDSLSDEAIDITLHDMEGYVSNAKVVILDACFNGSFHQDDYIAGHYIFNSGN
                     TMVVKGNSVNTLQDIWTNQLMGLFDLGVCIGNWAKGQMTLESHLFGDPTFHFTSSRPD
                     LNNINEAIVSRKDDEKFWRKAMKDANADYKALAMKMLYQANQISTDELLRIQKEENRS
                     VVRLEAFTLINKSYDKNLVPSIKLGLNDSYELQRRLAAISAANNSSPELLEQIVSIYM
                     QPGVSKRVEFQLKSAIENYPEELAFAAIDKAISNKNYQWYKDKMDEKKKFEYSFSGQK
                     KDFNALLDKSISAKEKKFTITSLRNSTNPTYLDTLFRFMKESTDNDLKQLLAEAFGWY
                     TQSWRRDDIVNFCQEQLNVEQNEQVKSELVRTINRLKN"
     gene            8614..11502
                     /locus_tag="P5293_03810"
     CDS             8614..11502
                     /locus_tag="P5293_03810"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRR49060.1"
                     /translation="MIKRKKQVSGRRDGILSFTVLVLFLTAGFCTDLSAQTTQGGKAT
                     LRGIVIEKGTNNPIEFATIQVLPQGSVATTDKKGEFIIEKLSPGKVNVKIFFLGMEPV
                     DTTFLMPAGIIEMKFEMQYSSFRLNEVSVVAKESKAGQATASNISRQAMDHLQTSSLS
                     DIMQLLPGGVIANPSLTTAKTFTIRTIDNSTTGANMNSLGTAIYVDGSPLSNNANLQA
                     LTPTITGSAGAVAGGASPNSGFDIRSISTDNIESVEVIRGIPSVEYGDLTSGAVIIRS
                     KAGREPFTVRFKTDPHIYQASVSRGISLGEKKGNLNISGDYAYSTADLTESYVYYQRV
                     TAKALYSNVFNNLVSNTSLDFTMGKDTRELNPNDARSQLTSGAKEYGFRFNTNGTWNI
                     NKGWLNNVKYALSANYTDKHSYRSELLGNAFAAYSMSNTDGAVLSNKPGQKVYDIYGH
                     ELTNIPPGESSYYATILPNEYKSRYDIYGDELNIFAKVNGTFTKTVGNVLNRIVIGAD
                     FKSDGNLGDGKVYDLANPPYRNLGSENSSPRPRKYSDIPFVNQLGIYAEENMVWSFGK
                     RELYIQAGGRYDNIAGKNFVAPRINASIEVIPDKFFIRGGYGVNAKAPTILYLYPENA
                     YFDFVHFNTLNSSIVPTNEQLLLISTRSFNTKNSDLKIASNKKSEIGIDLKIDKMRFS
                     VTAYDEKLKNGYYLWYSVDNFKLINYIQYEVGQANAGAIPLLKEKSRSNIFVSYASPM
                     NNTRSHSRGVEFDFDLGRFDQIRTSFVLNGAYMRTTDWNAGNKFGTNKNGNSLEKNIA
                     IYEPANSKDERERFTTTLRATHNIPAIGFVLTATAQVNWINKTWTTYGNDSVFVAYIS
                     YEDGLVHNLTENQIRELTNKQGPTYDQLEYMLDVKSAVRHIAESYFPALTINLNLTKE
                     IGELMKASFYVNNMFNTRPKYESKRYPGSFTRLNVPIYFGFEFTVTIK"
     gene            11519..>11924
                     /locus_tag="P5293_03815"
     CDS             11519..>11924
                     /locus_tag="P5293_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49061.1"
                     /translation="MKNKIKIFMVFLWAFAGLTACDQFDDNAHTKKVEPVRSVIVLDL
                     QLGDAPTPSEFNVKLINYNERYEINKTMSSDGMLTVDDVIPGIYTITVSAEMNEHGFT
                     YNYSGSVVNTEIIRSGVRQVVKVESSKAGNLVL"
BASE COUNT         3755 a         1978 c         2382 g         3809 t
ORIGIN      
        1 caatggaacc ttttcatccg gatctgatca ttgctaattg tccaggatgc actttctttt
       61 tagacaggtg gcagtatgtc atatctgaaa tcgaaggtaa gatatatgga gataatggat
      121 atggaattcc tgtgctgaca tatgaggaat tagctggact gcttatggga tataatcctt
      181 gggatgtagg cttacagctt catcaagtag cagtagaacc attattggat aaacttggtg
      241 tcaaatataa cccggatgac aagtataaag gaagagacgg gaagattttg aagttaccac
      301 aacaatcctt attaaagatg tattgaataa attttaataa ggatgattat ttcattccca
      361 atttctcaag cagagcttca ggtcttggat ccctgcccct gaagttgcga tacaaaacat
      421 ctgcatcaat aagattaccc tttgaaagga tattttcacg gaaggatgca gcaacctcct
      481 tgttgaaaat tcctttttct ttaaagagtg aaaatgcatc tgcttcaaga acttctgccc
      541 acttatagct ataatatccg gcagaatatc cgcctgagaa tatgtggctg aaagaggttg
      601 atgtacaggt agaatcaata agtggcatta attgggcttt ctgtgtggct tcacgttcga
      661 attccataac tcccttgtct ggagtctcag tgactgtatg ccatgccata tcatttattc
      721 cgaacgaaat ttgacgcaca gaattatatc cgcttaaaaa gtttttggag tcaacaatct
      781 tttgaatcat ttcctgagga ataatttcac cggtttcgta atgctttgca aaagataaaa
      841 gaaaatccgg ttctgttgcc caattctcca tgatctgcga aggcaattcc acaaaatccc
      901 gcttgacatt tgtacctgtc aggctggcat aacttccttt tgcaaacatg ccgtgaaggg
      961 catgcccgaa ttcgtgtaac attgtggtta cttcgtagaa tgtgagaaga gatggtttat
     1021 tctcagttgg ttttgtaaag ttgaaaacta gagtaacgaa tggtcgatat tcaactccct
     1081 tcataatata ctcctctctg aaatttgtca tccatgctcc actgtttttg ccttcacgag
     1141 ggaagtaatc tacatagagt aaagacaaga actttccgtc agcgtcaaaa acttcaaata
     1201 cttttacatc aggatggtaa acaggaagag atttgttttg gacaaatttc agtccgtaaa
     1261 gagaatcagc cagactgaaa actgcttttt caacattttc aagcttgaaa tatggtttta
     1321 aaagttcatc attgattgag aacttttcat gtttatattt ttcagaatag taactgaaat
     1381 cccacggcat taacttgcct ttgaatccat tggcatctgc ataggcttga atattcgcca
     1441 gatcttttct tgcataagga agtgacttgt caagtagatc tgacagaaat ttattaactg
     1501 tttcgggatt cttagccatg ttgtcagata gaatatattc tgcatgattt ttaaaaccga
     1561 gcagttttgc tcgtttcgct ctcagaccaa caattttttt gattatttcc tgattgtcaa
     1621 attcatcacc aaatgctttt ctatttgatg ctttccataa tttttcctta agttccctat
     1681 tctcactata ttgaagaaat gggccatagc ttggatattg aagggtataa acccaacctt
     1741 ctaaagaacg acttttagct tctgaagcgc ccatttcttt aacgaactca ggtaaaccca
     1801 caagttggga actgtcagta atgtgtaaga aaaacttatt tgttgctccc agaacattct
     1861 gatcgaattt ttgactcaac tgcgccagtt cattatcaat ttttttaagt tctgccttct
     1921 gtttgggatt taaatttgct ccatttcttg tgaaactctt gtaagtctct tcaagtaaac
     1981 gtttctgctc ctgattaagt tgcagggaat ccttagctga gtatacactt tttattctgt
     2041 taaacagctt ttcattgagc atgatatcgt tgccaaagtc agagagcatg ggagagactt
     2101 caagcgcaac cttctgcatg gatgagtctg tatttgaacc gtttaaagca aagaaaatgg
     2161 atgcaatatt actcaggttg cggcttgcaa attcgagagc ttcaacagta tttgcaaatg
     2221 ttggagcgtc aggattgtta ataattgaat ctatccttgc ctttgaagca gtaatagctg
     2281 cctcaaaagc aggtttgaaa tgaaatgttt tgattttatc gaaagcagga gcaccatatt
     2341 ggttgttcga tgttaacaac aatgggtttt ctgtcatttt acggtcacat gattgaagaa
     2401 taataagggc gatcaagacg attactgtgc caagcttagt catactttat tgaatttgga
     2461 ttacgagata tttagtctgt tcccaaaaag cctttggatc ggtaatttta agaacaagca
     2521 ttccggcttc atcctcttca atagaatatg ttcctgtcgg gtgtaatgac ataacttttg
     2581 ctttagttgc atttaacttg atctctctga gttccctgat gtcgattttc acaaatgctg
     2641 attgctctgc ttgataagaa atcttggctg atctgaacaa accgcctttt gacattacgt
     2701 tggctgcaat aaggtctgcc tttgtaccaa caatataata tccggtgttt atttgagctt
     2761 cttgtgatga gatagtctct ttctgagagg aactttctgc actcaggctg gaaacgtcgt
     2821 ttttgagtgt ttcaacagtc tcttcgagtg aggaaatctg ttgtgtcttg acttttatct
     2881 gattttcctt ctcttccagt tgtcttccga gatcagctat aagttctgat ttagcattaa
     2941 gttctgcttc gagagcatta agccttttca taaattgagc agactggatt ctgctgtcat
     3001 tttttagctt ttcaatttgt tttttgtaat tcttaattgc ttctccaatg gctgtgacgt
     3061 ctgacatcaa acgatctctg gaacttgcat taacttcagc tcctcctttt tgagactgca
     3121 atacaagtat attttcagct tcccttatag atttaagtcc attttcaacc tcattgagag
     3181 tagaaaatac ttcttcaaac tcggcacttt tagtggcagt taatgtggat aatgaatcca
     3241 tttgagcctg cagggcttta tacttttttg agctctccac gcaggatgac agagagatgg
     3301 cgaaaaaagt tattaataag actgtgagta attttttcat ggctttattt attaaagaaa
     3361 ttttcaaatt ttgttaaatt tttatgtttc aaaaatagct ataattttga tagattgtta
     3421 actattggac atttttgtga tcttaatcat attacgtgat tttattacaa agccagtgtc
     3481 ataacacaca aaacatgtct ctcctgaagg gtttatttga attgatgtga aatgcgttaa
     3541 atccactcct tcatcaatag cttgattaat atcgataaag acttcgtctt cggaaataaa
     3601 tttgttgaat atgcgattat tacggagaag aattttgttt acttcagcta ttctgataag
     3661 ctcagttttc ctttttttat tgtgaaaaga gtttctacac atatcagaac aaaatttctt
     3721 atcgcttctt cctcgtaact cctttccaca ttccttacaa attcccatat catttaatca
     3781 taattgtgaa gaattcaaat gtattgataa taaaacatca aaaagtatat aatcgccaaa
     3841 aattacgttg atttctaata tttgtttgat tttaaaaatt gataaaaata atataattga
     3901 tgaagtagtt agattaaacg tatataaacg tttataagtc atttaagata ttttttttgc
     3961 ttttatttgt ctcgatttta atttataaaa attacaagat ggaaaactgg gtaaataagg
     4021 tggagttaag aggaaatgtc ggctttgacc caaagctgat ttcacttgag gatggaggat
     4081 catttataag attttcactg gcaactaatg agacgtttaa gaacagaaaa ggcgaattgc
     4141 atgaggagac agtctggcac aacattattg catggaatgg caaaaacata cctgactttt
     4201 ctcaaattaa aaaaggcagt tacttgcatg ttaccggaag aattaagccg gttcagtacc
     4261 agacaaaaag cggcactgaa agacatacct atgaaattat tgcagtaagt atcaagcttc
     4321 tcgataggga aagcctcgaa taaaaatatt attgtaattt tacggcatta atcgaccgta
     4381 aaatgaggga ttttactatt tataacactc tttccagaaa aaaagaaaag tttgagacta
     4441 ttcatcctca aatggtaggc atatatgttt gtggtcctac tgtatatgga gatgcacatc
     4501 ttggacatgc aaggcctgct gtaacgtatg atgttctggt taggcttttc aggcatctag
     4561 gttacaaggt cagatatgta aggaacatta cagatgttgg tcaccttgag agcgatgcag
     4621 acgaaggaga tgataaaatc ttaaaaaaag caaagctgga acaactggag ccaatggaga
     4681 tagtacagag gtacacaaat cgctttcatg aatgtatgag attgctaaat acacttcctc
     4741 ctgatattga accgagagcc tcaggacata ttatcgaaca gattgcgctt gtgaaaacta
     4801 ttcttgataa tggctttgca tacgagagtc gtggttcggt ctattttgac gttgagaaat
     4861 ataataaaaa gtttaaatat ggagtattat ccggcagaca gcttgaggac cttcaatcaa
     4921 attcaagaag tctcgacgga caggatgata aaaggtctcc atatgatttt gctttatgga
     4981 aaaaggcttc gcctgagcac ataatgagat ggccttcacc ttggggcgaa ggattcccgg
     5041 ggtggcatct tgaatgttct gcaatgagtg caaaatatct gggtgaagag tttgatattc
     5101 acggaggagg aatggatctt cttttcccac atcatgaatg tgaacttgcc cagaatactg
     5161 cttcaagagg agttggtgga gttcgttact ggatgcataa taatatgatt accattaatg
     5221 gacagaaaat gggcaaatct cttggtaatt ttattaccct tgaggagctt tttgagggta
     5281 agcatcagat gctctcacag cctttttctc caatgacggt aaggtttttt attcttcagg
     5341 ctcattaccg aagtactctc gattttagta atgaggcttt ggtagcgtcg gagagaggtt
     5401 tgaaaaaatt gattcaggcc ttaaaagatg tagtaaaatt accagttgtt acagaaacct
     5461 cagagcttgg agttgattca ttaatcgaaa agatatatga ctcactgtgt gatgatttaa
     5521 acacacctgt cgcaatatct catatttttg atgcagtaaa gattgtcaat caagttaagg
     5581 aaaaacagct agtattatct caatcagaga aagagggcct tgtctatatt tttaatattt
     5641 tgttaacaga aatactcgga cttaaggatg ataaggttga aagaagtgat attgagattt
     5701 tagatgaggt aataaaatta ctgcttgaca taagatcaga ggctaaaagg aaaggagatt
     5761 ttacaacaag tgacaaaatt agaaataatt taaaagctct tggcattgaa ataagagata
     5821 ctaaagaggg gacggagtgg agctattaaa ggatatgatt tcatttatca tcttttttta
     5881 tatttttgct ttaactttaa aagggccttt aattgcgttc gggttttgtt atattatcaa
     5941 ttgtgagttc tgccatgtat cttgttgcca tcatgggtat tggcaggtat gcttgttttt
     6001 gtagccactc atcacattta gttttttggg ggataagttc aaaatgttcc tgtactcagc
     6061 ctgatcaccc ttgtgatgat ccgaaaaatt gttgcccttg ttgtggcaga catctcattt
     6121 cgcatagtgt taagaaaaat gattgctgca gagtaaagta caatttttta aggatagatc
     6181 aaagtcagtc cacagttaat ctgctttcag atattaaatc aacactggag gccataattc
     6241 ctgttcagag aataataatt gcgaagtggg tcaccacaga tttgataaaa aactttcagg
     6301 ctttgtttcg aagtgtcacg gaccatttat atgaaaaaca cggacaattg attttatgat
     6361 gagagtgaac tttggttaac atctctgtct ttttattgtt ttttattaca taacattatt
     6421 aaataaaata tgaagttttt tcccacaaaa attgttcttg tggcacttat aattgtagcg
     6481 gcagtaagtt atgcaagtgc caaatctcaa cctcgttctt ttgcaatcgt ggttgacaaa
     6541 aatacttatg aaaactgtcg tgcatcaatt gataaatatt ccacagctgt gaggcttgat
     6601 ggtcttaata cttttgtgat agtcgataaa tggaatattc ctgattctat tagaatggaa
     6661 ttgaaaagac tatatctaaa taacaatctt gagggagcag ttttaatagg tgatattcct
     6721 gttcctatga ttcgcgatgc gcaacatctt acaactgctt ttaaaatgga tcagcggcga
     6781 ccatggaatc tttcatcggt gccatctgat agattctatg atgattttga tctaaaattt
     6841 aaatttttaa aaagggactc cacaaataat ctctattttt attacaattt agcatctgac
     6901 agtccgcatc atattacatg cgatatttac agtgccagaa ttaaacctcc tacagtcaag
     6961 ggcaaggaca agtttcaact tattaatgag tatcttgaga aggctgcacg ggaaaaaggc
     7021 attcctcgat ctttaggcgc aattacctat tttgcaggtc acggctataa ttcagactgt
     7081 atggttgccc gtgctgatga gcgttatagt ctcaccgaac agttcccttt tgtagtggca
     7141 ggttataaca ggttgaacta tattgaccat acctatgata attatgtcaa atatcgtttg
     7201 atggcagagc ttgcgcgaga agatctcgga cttgcaatac ttcatcacca cgggtctgaa
     7261 gacaggcagt atttgaatgg aactcctctt gctacaactg cttcagactg gatcggactt
     7321 gctaaaaagt tttttcgtag caaaatcaga aattcagacg atactactgc aagcaaaaat
     7381 tattatatac gggaatatgg aataccggag agttgggtaa ataatgcgtt tgatcctgaa
     7441 atcgtacgac aggacagcct tagtgacgaa gctattgata taactcttca tgatatggag
     7501 ggatatgttt caaatgctaa agttgtaatt ctcgacgctt gttttaatgg ttcttttcat
     7561 caggacgatt atatagcagg acattatatt tttaattccg gcaataccat ggtagtcaag
     7621 ggtaattctg tcaatacttt gcaggatatt tggacaaatc agctgatggg gcttttcgat
     7681 cttggcgttt gtatagggaa ctgggcaaag ggacagatga ctcttgagtc tcatcttttt
     7741 ggtgacccta cttttcattt tacttccagc aggcctgatt tgaataacat aaatgaggcg
     7801 atagtgagtc gtaaggatga tgaaaaattt tggagaaaag ccatgaaaga tgcaaatgct
     7861 gattacaaag cgcttgccat gaaaatgctt tatcaagcaa atcaaatttc tacagacgaa
     7921 cttttgagga ttcaaaagga agaaaatcgc agtgtagtaa gacttgaagc attcacactt
     7981 attaataaaa gctatgataa aaacttggtt ccttcgatta aattaggatt aaatgacagt
     8041 tatgagcttc agagaagact tgccgcaatt tcggcagcta ataatagttc gccggaactt
     8101 ttggagcaga ttgtcagtat ttatatgcaa cccggagtca gcaaaagagt tgaatttcag
     8161 ttgaagagtg ctatagaaaa ttatcctgaa gagttagctt ttgctgctat agataaagca
     8221 attagtaata agaattatca atggtacaaa gacaagatgg acgagaaaaa gaagtttgaa
     8281 tattcatttt ccggtcagaa gaaagatttt aatgccctat tagataagtc aatcagtgcc
     8341 aaagagaaaa aatttacaat aacttcctta cgtaactcaa ccaatccgac atatttagac
     8401 acacttttcc gttttatgaa agagtcgaca gacaatgatc tcaagcagtt acttgccgag
     8461 gcatttggct ggtatactca gtcatggaga agggacgata ttgtcaactt ttgtcaggag
     8521 cagttgaatg tagagcaaaa tgaacaggtt aaaagtgaac ttgtcagaac aataaataga
     8581 ttgaaaaatt aacccacaaa tattttctgg actatgatta aacgcaaaaa acaagtatca
     8641 ggtaggaggg atggaattct ttcctttact gtcttagttt tgtttttgac agcgggattc
     8701 tgtacagact tgtctgcaca gacaactcaa ggcggaaaag caactctaag aggaattgtg
     8761 atcgagaagg gtacaaataa tcctatcgaa tttgccacaa ttcaggtact acctcaagga
     8821 tctgttgcca caacagataa aaagggggag tttattattg aaaaattgtc acccggaaag
     8881 gtgaatgtga agattttttt cttgggcatg gaaccagttg ataccacatt cttaatgcct
     8941 gcaggaatta ttgaaatgaa gtttgaaatg cagtatagtt cttttagact gaatgaagtt
     9001 tctgttgttg ccaaagagag taaggctggt caggccacag cttccaatat atcgagacag
     9061 gctatggacc acttgcaaac gagctctctt tctgatatta tgcaacttct accaggagga
     9121 gtaatagcca acccaagcct cacaactgct aagactttta ctattaggac aatagataat
     9181 tccaccactg gtgcaaacat gaacagtctg ggtactgcta tttatgtaga tggatctcct
     9241 ttgtctaaca atgccaatct acaggcttta acacctacca taacaggctc tgcaggtgca
     9301 gtggctggtg gagccagccc taattcagga tttgacataa ggtctatttc tactgacaat
     9361 attgaatcag ttgaggtcat aaggggtatt ccctctgtgg aatatggaga tttgacttca
     9421 ggggctgtaa tcataagatc taaagctggt cgcgagcctt ttactgtaag gtttaaaaca
     9481 gaccctcata tctatcaagc ttcagtgagc aggggaatat ctttgggtga gaaaaaaggt
     9541 aatttaaata tcagtggtga ttacgcatac agtactgctg accttacaga atcctatgta
     9601 tactatcaga gagtcacagc caaggctctg tattccaatg tttttaacaa tcttgtgtct
     9661 aatacttcac tggattttac aatgggtaag gacactcggg aattaaatcc taatgatgcg
     9721 agaagtcaac tgacttctgg agctaaggaa tatggattta ggtttaatac taacgggact
     9781 tggaatatta acaaaggatg gcttaataat gtgaagtatg cattatcagc taattataca
     9841 gataaacatt cctacagaag tgagcttttg ggtaatgctt ttgctgccta ttcaatgtcc
     9901 aatactgatg gagcggtttt aagcaataaa cccgggcaga aagtttatga catctatgga
     9961 catgagctta caaatattcc tccgggtgaa agtagttatt atgctactat attgccaaac
    10021 gaatataaga gtagatatga tatttatgga gatgaactga acatttttgc caaggtgaac
    10081 ggaaccttta caaaaactgt tggtaatgtt ttaaacagaa tagtaatagg tgcagatttt
    10141 aaatctgatg gcaatcttgg tgatggaaag gtgtatgatt tggcaaatcc gccttataga
    10201 aatttaggca gtgaaaactc ttctccgaga ccaaggaaat attcggatat tccttttgta
    10261 aatcaacttg gaatttatgc agaggagaat atggtctggt cttttggaaa gagagaatta
    10321 tatatacagg ctggtggaag atatgataat atagcaggaa aaaattttgt agcgcctcgt
    10381 attaatgctt caatagaagt aatacccgat aaatttttca ttcgtggagg ttatggtgta
    10441 aatgccaagg caccaactat cctctatctc tatcccgaaa atgcatattt tgattttgtt
    10501 cattttaata ctcttaatag ctctatagta ccaacaaatg aacagctgct attaatctct
    10561 accagatctt tcaataccaa gaattcagat ctgaagatag cgtctaataa aaagagtgaa
    10621 ataggtattg atttgaagat tgataaaatg cgcttttctg ttactgctta tgacgaaaaa
    10681 ctaaaaaacg gttactatct ttggtattct gtagacaact tcaagttaat taattatatt
    10741 caatatgaag ttgggcaggc taatgcaggt gcaatacctc tattgaaaga gaaaagcaga
    10801 agcaatattt ttgtaagtta tgcgagccca atgaataata caaggagtca tagcaggggc
    10861 gttgaatttg attttgatct tggaaggttt gaccagatta ggacatcttt tgttttaaat
    10921 ggtgcctata tgaggacaac agactggaat gccggtaaca aatttggaac taacaaaaat
    10981 ggtaatagtc tggagaagaa tatagcaata tatgaaccag ccaattccaa agatgagagg
    11041 gaacgtttta ctacaacttt gcgtgcaaca cacaatattc cagctattgg atttgttctt
    11101 actgctactg ctcaggtaaa ctggatcaat aagacctgga caacatatgg aaatgattca
    11161 gtatttgtag catatatttc atatgaagat ggtttagttc ataatcttac tgaaaatcaa
    11221 ataagggagc ttacaaataa acaaggtcct acttatgacc aactggagta catgcttgat
    11281 gtaaaaagtg ccgttagaca tattgcggaa tcatattttc cagcattaac catcaattta
    11341 aacctaacaa aggagatagg agaacttatg aaagcctcat tttacgtgaa taatatgttc
    11401 aatacacgtc ctaaatatga gagtaaaaga tacccgggtt catttaccag gttgaatgtg
    11461 ccgatttatt tcggctttga atttactgta actatcaaat agtctaaaat gatgtggtat
    11521 gaaaaataaa ataaagatat ttatggtatt tctgtgggct tttgcaggct taactgcttg
    11581 tgatcaattt gatgataatg cccatactaa gaaggtagag ccagtaagat cggtcatagt
    11641 attggatctt caacttggag atgctccaac tccttctgag tttaatgtta agctgatcaa
    11701 ttataatgag aggtatgaga taaacaagac aatgtcttct gatggtatgc tgacagttga
    11761 tgatgtgatt ccaggtatat atacaatcac tgtttctgca gaaatgaatg agcacggctt
    11821 tacttataat tatagcggaa gcgtggtaaa tactgagatt atcagatccg gggtacgtca
    11881 agtagttaaa gtagaatctt cgaaagctgg caatcttgtt ttaa
//
LOCUS       DAOTQL010000041        11789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_4994_length_11789_cov_4.599540, whole genome shotgun sequence.
ACCESSION   DAOTQL010000041 DAOTQL010000000
VERSION     DAOTQL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11789
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4994_length_11789_cov_4.599540"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="P5293_03820"
     CDS             <1..1463
                     /locus_tag="P5293_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRR49062.1"
                     /translation="KAYEITPEILLEDNPLLVHGLKEGSTILVRDLSKKVSFIKHTVR
                     WYDSLSSIAKKYGVSETDIIQVNSLKDDRLSVRQVLLIPDSSSISNPLSDDNIMQTDV
                     TSDEKVATSINYDQKEILTEKHVSANRHFADFRFNVSLILPLGSNKTEISEMDANFLD
                     FYEGFLLAVNDMKEEGMNINLKVLDMYDFPSGQILAQSGKLDNSHLIVGPVFEKEIAQ
                     IVSHTEREGIPLVSPMDSKTESLVSDHPFFYQINTNLYNIQMTLLKSIPKGSHVTIIY
                     EDSGEDRELIELTKRILSELGLTYNSISYNILSGRGVTAKIGDKLSPTSMNAVVVPSN
                     QEAFVSDVLRNLNLLQTINKFNITLYGTSKWRSFDNIDINYYHSMNLHIALQHFVDYN
                     DPNVKKFLLRYRALYNCEPNPYSFQAYDIAKYFLGQLLKEGPDFYKLQNHSTQRLLQS
                     DFKFERPDSDSGYINTATRLVIYNPDYSVNIGSLGL"
     gene            complement(1434..3224)
                     /locus_tag="P5293_03825"
     CDS             complement(1434..3224)
                     /locus_tag="P5293_03825"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRR49063.1"
                     /translation="MATYKLDLLRKAMKEQNVSAIIVPMADPHFGEYTQEHYKCIKWL
                     SGFDGSAGTIVVTMKDAALWTDSRYFLQATIQLANSGIELQRLKMPGTESIEVWLKGR
                     LQARERVGVVKDLFSFSEFSSLKSELVPLDLIEIEDIFQNIWKSRPAVEFYSIDQLDD
                     IYAGESISSKHSRVVEEIREEGDFIYILSTCDDIAWLFNIRGKDIKYNPVSFSYAAIT
                     REQLYLFVKNGTVCEELYKKLTNEGVSIREYDDFEQFLLNYSANAVRIAPPDKISLRK
                     YTISLLSGATFKSDTLRGGIVANLKAVKNPTEINGFRKAMILDGVAWVKYLRYIEKRL
                     QDVDNPLGEWEAAGMIGRFRAESDLYVGESFAPIVAFGHNAALPHYEPSEEIQSIIAK
                     DNFLLTDTGGQYICGTTDTTRTIAIGEITEEQKRDYTLVLAGMIDLAMAKFPTGTRGA
                     QLDILARGKVFSVGKMYLHGTGHGVGHYLCVHEGPQSIRMEDNLVTFKPGMVTSDEPA
                     IYEEGRYGIRSENLLLCREWCRNNAGTFLEFETLTLVPFDTRPILRNLLGEERVNWLN
                     EYHQRVFRELSPYLKNEDREWLKTKTANID"
     gene            3321..3899
                     /locus_tag="P5293_03830"
     CDS             3321..3899
                     /locus_tag="P5293_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRR49064.1"
                     /translation="MEKSIKGTKTEQNLLKSFAGESQARSRYVFFASVAKKEGFEKIA
                     DVFAETAEQEKEHAKRFFKFLEGGMVEITASYPAGVIGTTKDNLTAAAAGEYEEWHDL
                     YPAFADIAMEEGFPQIATAFKMISKVEAEHEKRYRTLLARVEEDKVFERDEEIEWQCR
                     NCGYVHKGKKAPENCPACNHPKAFFEPKKNNY"
     gene            3910..4854
                     /locus_tag="P5293_03835"
     CDS             3910..4854
                     /locus_tag="P5293_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRR49065.1"
                     /translation="MEGTIGERTLKAIREVLPSTLKTCIWLVKITVLISLGILILKYF
                     NILPWFSQLISPFFNVFGLPGEAALAYVTGYFVNVYSAIAVIATLDLDTRAITILSVM
                     VLCSHNMIIETAVQKKTGSSAVRIVIIRTLSAFILAFLLNLIMPGEVVSNVQHSQIDK
                     VSLTEMLEDWFFSTLRVVLTMTVLIFSLSVLQKILSEFGIIRWLSKIMRPFLAIFGLP
                     AKTSFLWIVANTLGLAYGAAVMIEEYKSGKVTKKDIDLLNHHIAISHSNLEDIILLAS
                     VGGMLWWMLLSRWCMSLLLVWELRCEMAIRKKLVKFAT"
     gene            4876..5811
                     /gene="trxB"
                     /locus_tag="P5293_03840"
     CDS             4876..5811
                     /gene="trxB"
                     /locus_tag="P5293_03840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRR49066.1"
                     /translation="MNTESVKCLIIGSGPAGYTAAIYASRANLSPVIYEGIQPGGQLT
                     TTTEVENFPGYPKGISGPELMEDFKKQALRFNADIRSGFVTSADLTERPFKLIIDEEK
                     EIIADTLIIATGATAKYLGLESEEKFRGQGVSACATCDGFFYKGKDVAVVGGGDTACE
                     EALYLSNLCRKVYMIVRRDVLRASKTMQERVKSTPNIEILWQYNTKEITGTDQGGVNG
                     AILVHKNGSERRIDIHGFFLAIGHHPNSELFSKWVKTNEEGYIVTEGKSSKTNVPGVF
                     AAGDVQDPNYRQAITAAGSGCMAALDAERFLCENK"
     gene            5819..6619
                     /gene="bamD"
                     /locus_tag="P5293_03845"
     CDS             5819..6619
                     /gene="bamD"
                     /locus_tag="P5293_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRR49067.1"
                     /translation="MKQGKLILIIATCVLLIASCKSPYEALLNSTDIQQKYKGAMTYF
                     DKGKYYKAAQLFEQLLLYVNGTDQDDTVQFYLGLSNYKYGDYINAEANFNEFVSVFPR
                     SPFTEEAKFLRIECLYNGTYRYELDQTPTFRTMAAINEYLYEYPNTNNLRRCRDMLVD
                     LQERLDRKSYEAAKLYYTLEDYKAASYALKNTLRENPDNQYREDIMYYIVCSSYKYAV
                     NSIPEKQKERFMVLIDDYYNFISEFPESKFRNELDGMFAEAQKITNKN"
     gene            6628..6942
                     /locus_tag="P5293_03850"
     CDS             6628..6942
                     /locus_tag="P5293_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRR49068.1"
                     /translation="MDIKKNVPTNTITRDVSKLCESTGNVYETTMIIAKRANQIAAIL
                     KQELDQKLKEFSNFADTLEETFENREQIEISRYYEKLPKPTLIAIAEYEEGCLNVRIA
                     EG"
     gene            6942..8159
                     /gene="coaBC"
                     /locus_tag="P5293_03855"
     CDS             6942..8159
                     /gene="coaBC"
                     /locus_tag="P5293_03855"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRR49069.1"
                     /translation="MMLEGKHILLGITGSIAAYKAAILARSLVKEGAEVKVIMTDMAK
                     DFITPLTMATLTKNPILVDHFDPQNGSWNSHIALGIWADLYVIAPVTANTIAKMSSGI
                     ADNLLLTTYLSARCPVMIAPAMDLDMFEHPATQNNLSTLSKRGVIVVEPSSGELASGL
                     EGKGRMEEPDIIVEQIKKILCPITSFSGKRILVTAGPTREEIDPVRFITNYSSGKMGY
                     AIAEEFAARGAEVLLVSGPTSLNTSSNLIKRIDVTSSEEMFQQTMEIYKNGVDIVVLC
                     AAVSDFTPNKRSETKIKREQEKNNKFILELKPTQDIAAELGKIKRPGTLHIGFALETD
                     NEVQNAFAKLDKKNLDAIVLNSLRDEGAGFGTDTNKISIIDIKGSIKDFNIKSKQLVA
                     VDIVNYIERLPEC"
     gene            8153..9793
                     /gene="recN"
                     /locus_tag="P5293_03860"
     CDS             8153..9793
                     /gene="recN"
                     /locus_tag="P5293_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRR49070.1"
                     /translation="MLKSLSIENYALIENLEIDFPEGFIIITGETGAGKSILMGAISL
                     LFGGKADPSILKNRDKNCIVEATFQIDADREITLRRVINHNGKSRSFIDDEPVSASTL
                     KELSEKLIDIHAQHQHLLLADSDFQLSVLDSYAENWQLLSEYQKDYELLNELTHNYNS
                     LKSKIEKEESESDYIKFQLNQLKEARLSPGELEELEEEQLLLNNAESIKKSLLTISNL
                     ISPEGSETSVLQNLKEAATLCSKLANDYKIVASLADRIETCRIELKDIEEEITNKAEE
                     IRIDPERSLIVEERLSELYTLLKKHHADKVEKLVEIMKELEDKSLMTDNHRIELEKME
                     NEIEECKIKRNQLAEKLHSRRVKASKGFSEVMLDSIKELEMPHASFYAEISQLSQYNP
                     YGNDSVTFFFSANKNIPPRELAKVASGGELSRIMLCLKAIMASGKGMPSMIFDEIDSG
                     VSGSIADKMGNLLYDLSKKMQIFAITHLPQIASKGNCHLLVFKDFDDKGVTKTNIKKI
                     EGEERVLEIARMLSGSELTQAAIANARELLKSDNLNMK"
     gene            9804..10628
                     /gene="nagB"
                     /locus_tag="P5293_03865"
     CDS             9804..10628
                     /gene="nagB"
                     /locus_tag="P5293_03865"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRR49071.1"
                     /translation="MRLIIQNTYEEVSQWAAAYIVKRINEFEPTKSRPFVLGLPTGST
                     PYGTYKELIRLYREGIVSFKNVVTFNMDEYIGIPREHPQSYFSFMWDNFFQYIDIERE
                     NANILDGNARDLELECENYEKKMAAYGGVNLFMGGIGPDGHIAFNEPGSSLSSRTRVK
                     SLTTDTIIANSRFFNNDIHQVPKTALTVGVGTIMDAEEVMILVNGHHKARALYEAVEG
                     SINHMYTVSILQMHRKGIIVCDDQSTVELKVGTVNYFKDIEKDNLDPKTLLNNLKK"
     gene            complement(10649..11680)
                     /locus_tag="P5293_03870"
     CDS             complement(10649..11680)
                     /locus_tag="P5293_03870"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HRR49072.1"
                     /translation="MKRTVTPSFYCKPDHEIEENRVFDYKIIEPELSPMRRTLNGKAD
                     SLILLFHGLNERNWDKYYPWAEYIAEKTRKPVLLFPIAFHINRAPDEWSAPRVMQPFI
                     SLENRDYIKNDHLTFLNYALSKRIQADPYRFYLAGRESVYNVCQLMNQIQKGRHPMID
                     ARASVDIFAYSIGAMLAQVLLFANPGYYFTDSKLFMFCGGSVFNEMNGDSRMIMDGES
                     FRTMIKYYTENFIYFKDEKRLKGDQLEHAFICHIDKSLYRSQREYFYNENAQRIRVLS
                     LKKDTVIPTSGIKSALGVEQLQCLEELDFPFNYSHEVPFPEVGPKVSATERRYWFERV
                     FSEVSDFLG"
BASE COUNT         3873 a         2251 c         2214 g         3451 t
ORIGIN      
        1 gcaaagctta tgaaattaca cctgagatct tgcttgaaga caacccgctt cttgttcatg
       61 gactcaaaga aggcagtaca atactggtga gagaccttag caaaaaagtt tcattcatca
      121 aacacactgt cagatggtat gattcgctat catctatagc taaaaaatat ggtgtttcgg
      181 aaacagatat tatccaggtg aactctttga aagatgatag attgtctgta aggcaggtat
      241 tgctgattcc tgactcttct tcaataagta acccgctgtc ggatgacaat attatgcaaa
      301 cagacgttac gtctgatgag aaagtggcaa cttctatcaa ttatgaccag aaggagattt
      361 taactgagaa gcatgtttct gccaatagac attttgctga ctttagattt aatgtttcac
      421 tcattctccc tcttggaagt aataaaacag aaataagcga gatggatgca aacttcctcg
      481 atttttatga gggttttttg cttgcagtca acgacatgaa agaagaaggg atgaatatca
      541 atcttaaagt tttggatatg tacgatttcc cttcagggca gatacttgcc caatcaggga
      601 agttggataa cagtcacttg atagttggcc ctgtatttga aaaagaaatt gctcagattg
      661 tcagccatac tgaaagagag ggaattccac tggtttctcc aatggattcc aaaacagagt
      721 cacttgtttc tgatcatcca tttttttacc agattaatac taatctttat aatatacaaa
      781 tgactcttct taaaagtatt cctaaaggtt ctcatgttac aattatctat gaagattcag
      841 gtgaagacag agaattaatt gaacttacaa aaagaattct ttccgaattg ggtttaactt
      901 ataactctat ttcctataac atcctgtcag gcaggggtgt gactgctaaa atcggagata
      961 aactctctcc aacatccatg aatgccgtag ttgttccatc aaaccaggaa gcctttgttt
     1021 cagatgttct gaggaatttg aatcttcttc aaacaatcaa caaatttaac attacattat
     1081 atggtacttc aaaatggaga tcctttgaca atatagacat taactactac cattcaatga
     1141 atcttcatat tgctttacaa cattttgtcg attacaatga cccaaatgtt aaaaagtttc
     1201 ttcttagata ccgggcatta tataattgtg aaccaaatcc atactctttc caggcatatg
     1261 atattgcaaa atatttcctg ggacaactat taaaagaagg acctgatttc tataaattac
     1321 aaaatcatag tactcaaagg ctgcttcagt ctgactttaa atttgaacga cctgactctg
     1381 acagtggata tatcaatacc gcaacgcggc ttgtaatata caaccctgac tactcagtca
     1441 atattggcag tcttggtctt taaccattcc ctgtcctcgt ttttaagata tggggagagt
     1501 tctctgaata ctctctgatg atattcatta agccagttca ctctttcttc accgagcaaa
     1561 tttcttaata tcggccttgt gtcaaaagga acaagagtca atgtttcaaa ttcgagaaat
     1621 gtgccggcat tgttcctgca ccattcacgg cacagaagaa ggttttcgct tctgattcca
     1681 tatctgcctt cttcatatat ggcaggttca tctgatgtaa ccatacctgg tttgaatgtg
     1741 acaagattat cttccattct tatgctttgc ggaccttcat gaacacaaag ataatgaccg
     1801 acaccgtgac ctgttccgtg caaatacatc ttgccgacag aaaaaacttt tcctcttgcc
     1861 aaaatgtcta attgagctcc tcttgtgcct gtcggaaatt ttgccattgc aagatcaatc
     1921 attcctgcga gaacaagagt ataatccctc ttttgctctt cagtaatctc tccaattgca
     1981 atggttctgg ttgtatctgt tgtgccacaa atgtattgtc cgcctgtatc tgtaagcaaa
     2041 aagttgtctt tggcaataat tgattgaatc tcttctgaag gttcataatg aggtaatgca
     2101 gcattatgac caaaagcaac aataggagca aaactttcac ctacataaag atcagattcg
     2161 gctctaaaac gtcctatcat tcctgcagct tcccactcac cgagaggatt gtctacatcc
     2221 tgaagccttt tttcaatgta tctaagatat ttcacccagg cgacaccatc taaaatcata
     2281 gcttttctaa atccgtttat ctctgttgga ttcttgacgg ctttgagatt tgccactatt
     2341 cctcccctca gggtgtcaga tttaaaagtg gctccggata ataaagaaat agtgtatttc
     2401 ctgagagaaa ttttatcagg aggagctatt cgtactgcat tggcagagta atttaaaaga
     2461 aactgttcaa aatcatcata ctctctgatt gatacccctt catttgtcag ttttttgtaa
     2521 agctcttcac aaacagttcc gttcttaaca aaaagatata gctgttctct tgttatggct
     2581 gcatatgaaa aagagacagg attgtatttt atatccttac ccctaatgtt gaagagccaa
     2641 gcaatatcat cacaggtaga taaaatataa ataaaatcac cttcttctct tatctcttcc
     2701 acaactctgc tgtgcttgga cgatattgat tctcctgcat atatgtcatc aagttggtca
     2761 atactataaa actcgacagc aggacgggat ttccatatat tctgaaaaat atcctctatc
     2821 tctatcaaat caagtggaac taattcactt ttgagagaag aaaattccga aaaagaaaat
     2881 aaatccttaa caacgccaac ccgttctctt gcctgaagtc ttcccttcaa ccaaacttca
     2941 atactttcag taccaggcat ttttaatctc tgtaattcaa tgcccgaatt tgcaagctga
     3001 attgtagctt gaagaaaata tcttgaatct gtccaaagtg cggcatcctt cattgtaaca
     3061 acaattgtcc cggcagatcc atcaaaccct gaaagccatt ttatgcactt ataatgctcc
     3121 tgagtatact cgccaaaatg tggatcagcc ataggtacta ttattgcgga aacattctgc
     3181 tctttcatcg ccttgcgcaa aaggtctaat ttataagttg ccatcttcaa aataatattt
     3241 gtattgaatg cgaatataca aattcatcaa ggatttttta cctttgtgaa taatgattaa
     3301 caacttttaa aataagagtt atggaaaaga gtattaaagg cacaaaaaca gagcaaaatc
     3361 ttctcaaatc atttgctggc gagtcacaag ccagaagcag atatgtcttt tttgcaagcg
     3421 ttgccaaaaa agaaggtttt gaaaaaatag cggatgtttt tgcagaaaca gctgaacagg
     3481 agaaagagca tgcaaaaaga tttttcaagt ttcttgaagg aggtatggta gaaattactg
     3541 catcctaccc tgccggcgtt ataggaacta caaaagataa tcttacagct gcagcagcag
     3601 gtgaatatga agaatggcac gacctttatc ctgcttttgc agatattgca atggaagaag
     3661 gattccctca aattgccact gccttcaaaa tgatttcaaa ggttgaagca gaacatgaaa
     3721 aacgctacag aacactgtta gccagagtcg aagaggacaa agtgtttgaa agggatgaag
     3781 aaatagaatg gcaatgccga aactgtggct atgttcataa aggtaaaaag gctcctgaaa
     3841 actgtcctgc ctgcaaccat cctaaagcat ttttcgaacc taagaaaaac aactactaac
     3901 atccaaactt tggaaggaac tatcggagaa agaacgctga aggctatacg ggaagttctc
     3961 ccatccacgc ttaaaacatg catttggttg gttaagataa ctgtgttaat ctcccttggg
     4021 atattgattc tcaagtattt taacattctt ccatggtttt cacaacttat aagtcccttt
     4081 ttcaatgtat ttggacttcc cggtgaagca gcgctggcat atgttacagg atattttgtc
     4141 aacgtctatt ctgcaatagc agtcattgcc acacttgatc tcgatacaag agccatcact
     4201 atattatctg tgatggttct ttgttcacat aacatgatca ttgaaactgc agtacaaaaa
     4261 aagaccgggt cttctgcagt cagaatagtt attatcagaa ctctgtctgc ttttattctg
     4321 gcttttttgc ttaatctcat aatgccaggt gaagttgtaa gtaatgtcca gcactctcaa
     4381 atagataagg tctctctaac agaaatgctt gaagactggt ttttttccac tttaagagtt
     4441 gtccttacaa tgacagtcct tatcttttcg ctttctgttt tacaaaaaat actgtcagaa
     4501 ttcggaatta tcaggtggct ctcaaaaata atgcgtcctt ttcttgctat tttcgggctt
     4561 cccgcaaaga cttccttttt gtggattgtt gcaaacacac ttggacttgc atatggcgca
     4621 gctgtaatga ttgaagagta taaaagcggc aaggttacaa agaaagatat cgaccttctc
     4681 aaccatcata tagctatctc gcacagcaac cttgaggata tcattttact tgcatcagta
     4741 ggaggaatgt tgtggtggat gttactttca agatggtgta tgtcgcttct tctggtctgg
     4801 gaattaagat gtgagatggc cataagaaaa aaattagtta aatttgccac ctgaataatt
     4861 aatcctcaac acacaatgaa cactgaaagt gtcaaatgtt tgataatagg cagtggacct
     4921 gccggttata cggcagcaat ctatgcctca agagcaaatc tctcccccgt catatacgaa
     4981 ggtattcaac ccggagggca acttacaaca accactgagg tcgaaaattt tccgggttat
     5041 cctaaaggga tttccgggcc tgaacttatg gaggatttta aaaaacaagc tcttagattt
     5101 aatgctgata ttcgctcggg ttttgttact tctgccgatt tgactgaaag accatttaaa
     5161 ctcattattg acgaagaaaa ggaaatcatt gcagatacat tgataattgc taccggggct
     5221 acagccaagt atctcggcct cgaatctgaa gagaagttta gaggtcaggg agtatctgcc
     5281 tgtgccacct gtgacggttt tttttacaaa ggaaaagacg tggcagttgt tggaggcggt
     5341 gatacagcat gcgaagaggc tttatatctt tccaatctct gtcgcaaagt atacatgatt
     5401 gtgagaagag atgtgctgag agcttccaaa acaatgcaag aacgggttaa aagcactcca
     5461 aatatcgaaa ttctctggca gtacaataca aaagagataa caggaactga tcaaggaggc
     5521 gtgaatggtg cgattcttgt tcataagaat ggttcggaaa ggagaattga catccacggt
     5581 ttctttcttg ccattggtca tcatcccaat tcggaacttt tcagcaaatg ggtgaaaaca
     5641 aatgaagagg gatatattgt gacagaaggc aagagcagca aaacaaatgt acccggcgta
     5701 tttgccgcag gtgacgtaca agaccccaat tatcgacagg ccataacagc tgccggttct
     5761 ggctgcatgg cagccttgga tgcagaaaga ttcctttgtg aaaataaata agttatttat
     5821 gaaacagggt aaacttatat taattatcgc tacgtgcgta ttattgattg cgtcatgcaa
     5881 atctccttat gaggcactgc ttaattctac tgatattcag caaaagtaca aaggagcaat
     5941 gacttatttt gacaaaggaa agtactataa agcagcgcaa ttgtttgaac agttgctatt
     6001 atatgttaat gggacagatc aggatgacac tgttcaattc tatcttggat taagtaacta
     6061 caaatatgga gattatatta atgctgaagc aaatttcaat gaatttgtaa gtgttttccc
     6121 aagaagtcct tttacagaag aggctaaatt tctgaggatt gaatgcctct acaatggaac
     6181 ataccgatat gaacttgatc aaactccaac tttcaggact atggctgcga taaatgaata
     6241 tctctatgaa taccccaata ctaacaatct cagacgatgc cgcgatatgc ttgttgacct
     6301 tcaggaaaga cttgacagaa aaagttatga agcagcaaag ttatattata cccttgaaga
     6361 ctataaggct gcaagttacg ctctcaagaa cacacttagg gagaacccag ataatcagta
     6421 ccgtgaagat ataatgtatt atattgtctg ttcaagttat aaatatgctg tcaatagcat
     6481 acctgaaaag cagaaggagc gttttatggt acttatagat gattattaca attttataag
     6541 cgaattccct gaatccaaat tcagaaatga gctggatggg atgtttgccg aagctcagaa
     6601 aataacaaac aaaaattaaa aacagatatg gatattaaaa aaaatgttcc caccaatact
     6661 attacaaggg acgtttcaaa actttgtgaa tcgacaggta atgtttatga aacaactatg
     6721 attattgcca aaagggccaa tcaaatcgct gctatactca aacaggaact ggatcagaaa
     6781 cttaaggagt tttccaactt tgccgatact ctcgaagaga cttttgaaaa tagagaacaa
     6841 attgagatct cacgctatta tgagaaattg cctaagccta ctctaattgc aattgccgag
     6901 tatgaagagg gctgcttaaa cgtgagaatt gccgaaggct gataatgctt gaggggaaac
     6961 atatattact cggtataacc ggtagtatag ctgcatacaa ggcagctata ctggctcgtt
     7021 cacttgttaa agaaggggct gaagtcaaag tgattatgac tgatatggcc aaggatttta
     7081 ttactcctct taccatggct acccttacaa aaaatcctat actggttgac cattttgacc
     7141 cgcaaaacgg cagttggaac agtcacatag cactagggat atgggctgat ctctatgtta
     7201 ttgcccctgt tactgcaaat actattgcaa aaatgtcttc aggtattgca gacaacctgc
     7261 tgttgacaac ttatttgtcg gctcgctgcc ctgttatgat agcccctgcc atggatcttg
     7321 atatgtttga acaccctgct actcaaaaca atctaagcac tctttcgaag cgtggagtta
     7381 ttgtagttga accatcttct ggagaattgg caagcggctt ggaaggaaaa ggaaggatgg
     7441 aggagccaga tattattgtt gaacagataa aaaaaattct ttgcccaatt acttcttttt
     7501 ccgggaaacg cattttagtc acagctggcc ctaccagaga ggagatagat ccagtaagat
     7561 tcatcacaaa ctactcttca ggcaaaatgg gatatgcaat tgccgaagaa ttcgctgcaa
     7621 gaggtgcaga agttttgctc gtcagcggtc ccacttcctt aaacacatct tccaatctta
     7681 tcaaaaggat agatgttaca tcttctgaag aaatgtttca gcaaaccatg gagatataca
     7741 aaaatggggt tgacattgtt gttttgtgcg ctgctgtgtc agatttcacc cctaataaga
     7801 gaagtgaaac aaagataaag cgggagcaag aaaaaaataa taaatttatt ttggaactta
     7861 agccaacaca ggatattgcg gccgaattag gtaaaattaa acgtccaggt acactccata
     7921 taggatttgc ccttgaaaca gataacgagg tacaaaatgc ttttgcaaaa cttgacaaaa
     7981 agaatcttga tgccatagtg ctgaattctc tcagagacga aggtgccggt tttggaaccg
     8041 acaccaataa aatctccatc attgacataa aaggttcaat caaagatttc aacattaaat
     8101 caaaacaact ggtagctgtt gatattgtaa attatattga gagattgcct gaatgctaaa
     8161 atctctttct attgaaaatt atgctctaat agagaatctg gaaattgatt tcccggaagg
     8221 atttatcatt ataacaggtg agaccggcgc aggtaagtct attctgatgg gagccatttc
     8281 acttttgttt ggtggcaaag ccgatccatc cattttgaaa aacagagata aaaactgcat
     8341 cgttgaagcc acattccaaa tcgatgctga cagagagata acccttagaa gagtaatcaa
     8401 ccataacgga aaatcaagat catttataga tgatgaacct gtttcagctt caactctcaa
     8461 agaactttct gaaaaactta tcgatattca tgcgcagcac caacatcttc tactggctga
     8521 ttctgatttt caactttcag tactggactc atatgcagag aattggcaac ttttgtcaga
     8581 gtaccaaaaa gattatgaac tgcttaacga gcttacacat aattataatt cccttaagtc
     8641 taaaattgaa aaagaggagt cagaatctga ctacattaaa ttccagctca accagctcaa
     8701 agaagccaga ctttctcccg gagaacttga agaacttgaa gaagaacaac ttctattaaa
     8761 taatgcagag tcaataaaaa aatcacttct tactatctca aatctgatat cccctgaagg
     8821 atcagaaact tcagttctgc aaaatcttaa agaagctgca actttatgtt ccaaattagc
     8881 aaatgattat aaaattgttg cctctttagc tgacagaata gaaacttgca ggatagaact
     8941 aaaagatatt gaagaggaaa taacaaacaa agcagaagaa ataagaatag atcctgaaag
     9001 aagtctaatt gtggaagaaa gattatctga actttatact cttcttaaga aacatcatgc
     9061 agacaaagtt gaaaagctgg tagagataat gaaagaactc gaagataaaa gtcttatgac
     9121 tgataatcac aggatcgaac ttgaaaaaat ggaaaatgag attgaagaat gtaaaataaa
     9181 gagaaatcaa cttgctgaaa agctgcactc tcgtagagta aaagcttcta aaggattctc
     9241 agaggtgatg ttggattcta taaaagagct ggaaatgcca catgcttcat tttatgcgga
     9301 gatatctcaa ctgagtcaat acaatcctta tggtaatgac agtgttacat ttttcttttc
     9361 agccaataaa aatatacctc cgagagaact tgccaaagta gcttcgggag gagaattatc
     9421 cagaatcatg ttatgcctta aggctattat ggcatcagga aaggggatgc cgtcaatgat
     9481 ttttgacgaa attgacagtg gggtttcagg aagtatcgca gacaaaatgg gtaacctttt
     9541 gtatgatctt tcaaaaaaga tgcagatatt tgcaataaca cacctgccac aaattgcatc
     9601 aaaagggaac tgccacctgc ttgttttcaa agactttgac gacaagggtg ttacaaaaac
     9661 caatattaaa aaaatcgaag gagaagagcg agttcttgaa attgcaagaa tgttgagtgg
     9721 atcagaactc acacaagcag caattgcaaa cgcaagagaa ttactcaaaa gtgataactt
     9781 aaatatgaaa taacaataaa actatgagat tgattattca aaacacttac gaagaggtct
     9841 ctcaatgggc tgctgcctat atagttaaga gaatcaacga atttgaacct acaaaatcca
     9901 gaccttttgt tctcggcctt cccacagggt ctactcctta tggtacttat aaagagctaa
     9961 tcagacttta tagagagggg atagtttcct ttaaaaacgt agttacattt aacatggacg
    10021 aatatatcgg aatacctcgc gaacatccac aatcttattt ttcgttcatg tgggataatt
    10081 tttttcagta tatagacatt gaaagagaaa atgcaaatat ccttgacgga aatgctcgtg
    10141 atctggaatt ggaatgcgaa aattatgaga aaaaaatggc cgcttatgga ggtgtgaatc
    10201 tttttatggg aggaatcggt cctgacggtc acattgcttt caacgaacca ggctcctcac
    10261 tttcatcccg caccagagtt aaaagtctta ctacagatac tatcattgca aactcaaggt
    10321 tctttaacaa tgatatccat caggtaccaa aaactgctct taccgtagga gtgggtacaa
    10381 tcatggatgc tgaagaggta atgattcttg taaatgggca tcacaaagca agagctttgt
    10441 atgaagccgt tgaaggttca ataaatcaca tgtatacagt gagtattctt caaatgcaca
    10501 gaaagggcat aattgtctgt gacgatcaat ctacagtgga actaaaagtg ggcactgtaa
    10561 actattttaa agatattgaa aaagacaatc ttgatcccaa aactcttctc aataatctaa
    10621 agaaatagaa ccggttttgc cactgttctt atccaagaaa gtcggaaacc tcagaaaaaa
    10681 ctctctcaaa ccagtatctc ctttcagtgg cagatacttt aggtcctacc tcagggaaag
    10741 gaacttcgtg agaatagtta aacggaaaat caagttcctc aaggcattga agttgttcta
    10801 caccaagagc tgacttgata ccgctcgtag gtattactgt gtcctttttg agagacaaaa
    10861 ctctgatcct ttgggcattt tcattataaa aatactccct ctgacttcta tacagtgatt
    10921 tgtctatatg acatataaac gcgtgttcaa gctgatctcc tttcagtcgc ttttcatcct
    10981 tgaaatagat aaagttttct gtatagtact ttatcatcgt tctgaaagat tctccatcca
    11041 taatcattct ggaatcacca ttcatttcat tgaaaacaga ccctccacaa aacatgaaaa
    11101 gtttactgtc ggtaaaataa tacccaggat tagcaaaaag aagcacctga gccagcattg
    11161 cacctattga atatgcaaaa atatccacac tcgcccttgc atcaatcata gggtgtctac
    11221 ccttttgaat ctgattcatc aactgacata cattatagac actttccctt ccggccagat
    11281 aaaatctata aggatctgcc tggatgcgct tgctcaaggc ataattcaaa aatgtaagat
    11341 ggtcattttt tatatagtct ctgttctcaa gcgaaataaa tggctgcatc acacgcgggg
    11401 cactccattc atctggagct ctgttgatat gaaaagctat tggaaaaaga agcaccggtt
    11461 ttctcgtctt ttcagcaata tattctgccc aaggataata tttgtcccaa tttctttcat
    11521 taagaccgtg aaaaagtaaa atcaaagaat cggctttccc gtttaaagtt cttctcatgg
    11581 ggcttagttc cggttcgata attttatagt cgaaaactct gttctcttca atttcatgat
    11641 cgggtttaca ataaaaagag ggggtaactg ttcgtttcat atcctcactt atctcttctc
    11701 ttccgagaat ttctctaaat aaaaatttat gagatttcat ggttgctctg tttttgacaa
    11761 aaaaacatta atcatataac tataacaaa
//
LOCUS       DAOTQL010000042        11711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5034_length_11711_cov_4.774194, whole genome shotgun sequence.
ACCESSION   DAOTQL010000042 DAOTQL010000000
VERSION     DAOTQL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5034_length_11711_cov_4.774194"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="P5293_03875"
     CDS             complement(<1..700)
                     /locus_tag="P5293_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRR49073.1"
                     /translation="MIKITFPDGSVRQYEKGITPYEIAQSISPKLAQEVLVATVNGKI
                     CELNRPINEDAAIKLHKWEDEEAKRTFWHSSSHLMAEALELLYPGVKFGIGPAIEIGF
                     YYDIDLGDRVIAESDLKRIEDKMLELAKEKQHFVRREVSKEEAMKTYSDKGDQYKCEL
                     IRDLEDGTITFYTNGSFTDLCRGPHLVDTSVIKAVKLTSIAGAYWRGNEKNKMLTRIY
                     GVTFPQKKLLDEYLI"
     gene            833..1351
                     /locus_tag="P5293_03880"
     CDS             833..1351
                     /locus_tag="P5293_03880"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRR49074.1"
                     /translation="MQSSQWRTAAINALLLSIITIVVSLIQVVFKPGTVLSILLWIIK
                     FIGCLALLYYFMKEFGKELESFSYKDGFNYGFKICLLSSIVLAAFNFLQYAILFPESL
                     QDAVEQVAMAMQSANPAGAEAIERYQAKLPQIITVTSFFYYTVFGLIASAIIANYTKK
                     ETFPTDNYQQTL"
     gene            1358..2311
                     /locus_tag="P5293_03885"
     CDS             1358..2311
                     /locus_tag="P5293_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR49075.1"
                     /translation="MDLSIIISVYNEEESLVELIPWIEKALYGKRIEYEIIIIDDGST
                     DSSWKVIEDLSRKYPSLIRGVSFRRNYGKSAALFCGFEAARGDVVVTMDADLQDSPEE
                     IPALFDMINSGQYDLVSGWKKKRRDNFIAKNIPSKIYNATARMVTGIKLHDMNCGLKA
                     YRKEVVKNIEVYGEMHRYIPYIAKNSGFTRIGEKVVIHRPRKFGKSKFGVDRFMNGFL
                     DLLTLWFLSKFGKRPMHFFGLIGSLTFVIGAITSIWLIVEKISAQSKGLYYRAVTDQP
                     LFYISLVALIVGLQLFLAGFVGELVTRNSSERNNYKIKATI"
     gene            complement(2324..2734)
                     /locus_tag="P5293_03890"
     CDS             complement(2324..2734)
                     /locus_tag="P5293_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRR49076.1"
                     /translation="MSKVESVTQVNEVNRISVGTEFRGKLISSCDIRIDGYFEGDLNT
                     TGKLVIGESGKLIGNVISRSCDIWGTLQGKVVVRETFGLKKSGSITGNLFCQRIFIED
                     GGLFNGSCKMIDENTFGELSGGELPGKQEHLEEN"
     gene            complement(2753..4141)
                     /gene="lpdA"
                     /locus_tag="P5293_03895"
     CDS             complement(2753..4141)
                     /gene="lpdA"
                     /locus_tag="P5293_03895"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRR49077.1"
                     /translation="MYDIIVLGSGPGGYVAAIRASQLGMKCAVVERAELGGICLNWGC
                     IPTKALLKSAQVLNYAKHAGEYGLNISCNVSADISKMVSRSRDVASGMSRGIEYLFKK
                     NGISVYFGNGKIVQPGKVKVDLNDGTSTQLEASNIVIATGSRANSLPFAPIDGEKIIS
                     YRQALVPEIIPASMVVIGSGAIGSEFAFLYRSLGSEVTLIEYLDNIVPLEDEEVSAQL
                     SRSFRKMGIKILTSARVNSVEKNDSGCKVEVLTKKGTEYIEAEKVLSAVGVIANTENL
                     GLENCGVVVERGKIRVDPFYSTNVKGIYAIGDVIATPALAHIASAEAICCVEKIAGLD
                     SKPLDYNNFPACTYTSPEIASVGMTEKAVKESGINYKVGKFFFTASGKAASSGEKDGF
                     VKLIIDAESDNILGAHLIGANVTEMISGIVVARSLGVKARDIAHSIHPHPTMSEAIME
                     AAAAAHGEVIHL"
     gene            complement(4154..7447)
                     /gene="secA"
                     /locus_tag="P5293_03900"
     CDS             complement(4154..7447)
                     /gene="secA"
                     /locus_tag="P5293_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRR49078.1"
                     /translation="MAFINGILKKFFGTKSEKDMKLIQPYVDKTKEAFERIDSLTHDE
                     LRAETQKIKKIITDYVAEDEAKKKSLRKKLEDLTIDISEKESLATEVDKLTKKIDEKL
                     EEVLLQVLPDAFAIMKSTARRFKENNEIVVTATQSDKDLSAEHDFVRIEEDKAIWKNT
                     WMAGGNRIQWDMVHYDVQLVGGVALHLGKIAEMATGEGKTLVATLPVFLNALAGKGVH
                     IVTVNDYLSKRDSEWMGPLYQFHGLKVDCIDKHEPNSDSRKAAYRADITFGTNNEFGF
                     DYLRDNMAVNQDDIVQRKHHYAIVDEVDSVLIDDARTPLIISGPVPRGDDQQFVEYKP
                     IVEKLYAAQRSIVTQVLNEARKKISEGNDEEGGLLLLRAHKGLPRYNPLIKYLSEPGI
                     KQLLLKTENFYMQDNNKQMHIVTDPLYFVIDEKQKSVDFTDAGNEFVSKIVDDPKFFI
                     LPDIGSEISELEKKNISDEEKAAAKDALIADYALKSERVHTVSQLLKAYTMFEKDVEY
                     VILDNKIKIVDEQTGRILEGRRYSDGLHQAIEAKENVKVEAATQTFATITLQNYFRMY
                     HKLAGMTGTAETEAGEFWSIYKLDVVVIPTNRPVIRKDMEDLIFKTKREKYNAVIEEI
                     VRLTNLNRPVLVGTTSVEVSELLSRMLKLRGIRHNVLNAKQHAKEAEIVAEAGQPRSV
                     TIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHDSRRVDRQLRGRAGRQGDPGSSSFY
                     VSLEDDLMRLFGSERIAGLVDRMGMKDGEMLQHSMLSKSIERAQRKVEENNFGIRKRL
                     LEYDDVMNSQREVIYTRRRNALYGERVDVDVQNMITDYVENFVVSHHGSSNFDDFVNE
                     SIRNLSIEVGMDETFYEGSSLNEVTEAYLNHVNDILKRRRDTLLQQSWPIIKQIYESQ
                     GAVYQNIAIPVSDGHKMLQMIVDLKKAYETNGLEVVRALNKSITLMMIDEHWKEHLRD
                     MDDLKQSVQNATYEQKDPLLIYKFESFELFKTMLDKISRDVISFLLKAHIPLRENTQQ
                     PPQLNLRQEARRKAGLNNIRTSREELSTSGGEPKSQGPVHVEKKVGRNDPCPCGSGKK
                     YKNCHGANL"
     gene            complement(7517..8326)
                     /locus_tag="P5293_03905"
     CDS             complement(7517..8326)
                     /locus_tag="P5293_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HRR49079.1"
                     /translation="MIIKISRSQSLFKIFITAVLLFAGVLDRVSLAQMVVKAEVCRCK
                     IVEVLPHDVNSYTQGLFFHKGQLYESSGQYGASFFRKVDLSRGTALQSFNFPDEYFAE
                     GATALGDLLYILTWQQRVVFVYDINTFKPIKQFFNPREEGWGLTTDGKDLIMSDGSSN
                     LFFMDPATFKEKRRIAVKLEGKPVDQLNELEYIKGEIWANVYQEDFILIIDPSTGAVR
                     KVVDCKNILPTSMRTSKTDVLNGIAYNPLTDKIYITGKYWPKMFRITLSSR"
     gene            complement(8323..9957)
                     /locus_tag="P5293_03910"
     CDS             complement(8323..9957)
                     /locus_tag="P5293_03910"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRR49080.1"
                     /translation="MSTKYVFVMGGVTSSLGKGIIASSLAKLLQARGLSVTIQKFDPY
                     INVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLNTFTSKANNVTTGKIYRTVIDK
                     ERQGAYLGKTVQIIPHITDEIKRRMLLLGKSGNFDVIITELGGTVGDIESLPFIEAVR
                     QLQWELPDEDCLVVHLTLVPYLNAAKELKTKPTQHSVRALQQNGVHPDIIVCRTEHPL
                     PAEIRRKVALFCNVRPNAVIESIDAPSIYEVPLNMYKEKLDELVLRKFKLDSYLTRNL
                     ELVKWKEFLDRIHNPKDTVDIAIVGKYVELQDAYKSILESFIHAGAENECKVNVHYVH
                     SEYVNENNVTEKLAAMHGILVAPGFGERGIQGKIISIKYAREQGIPFFGICLGMQMAV
                     VEYAQNVLGLEDAMTTEIKQNAKNPVIDLMEDQKSLTIKGGTMRLGAYRCDIEEGSLA
                     YSIYGKKEISERHRHRYEVNSNYVEALENGGLHASGKNPDTGLVEIVELPGHPFFIGV
                     QFHPELKSTPERPQPIFVAFVKAALKHKHKNDSSNI"
     gene            10045..10764
                     /locus_tag="P5293_03915"
     CDS             10045..10764
                     /locus_tag="P5293_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRR49081.1"
                     /translation="MKIYSEKYLVKSYETDMNANLKLFAFMNYAQEIAGQHANLLGFG
                     YDTLIKEGVVWVLSRMHIIFNDIPKWKDNITMYTWHKGGDRLFWYRDFVVNNEIEETV
                     IKATSSWVIINVETRKMHKIELLGHPDTINSEDAITEHAEKIITPHEMEYVHTKKVSY
                     SDVDINNHTNNAKYVEWAIDSLDPEYYSSIQLSDMIINFNLETRLNDQVEIYRHVSDN
                     NVIIEGKREKNSIFTIEMIIK"
     gene            complement(10776..11234)
                     /locus_tag="P5293_03920"
     CDS             complement(10776..11234)
                     /locus_tag="P5293_03920"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRR49082.1"
                     /translation="MKTIALVAHDNRKKDLMEWVEFNKETLKKHKLVCTGTTGRMVRE
                     TLSKGTHNEGKELDITILKSGPLGGDQQLGALIAQEEIDLLFFLWDPMQPQPHDVDVK
                     ALLRISSLYNVPTATNRSTADFLISSPLFDTDYHPKLISYEDYVTRKLIL"
     gene            complement(11251..11703)
                     /locus_tag="P5293_03925"
     CDS             complement(11251..11703)
                     /locus_tag="P5293_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR49083.1"
                     /translation="MEFIIRAAKPDDTGIILGFIYQLAEYEKLKHDVTATEEILYDSL
                     FVKKSAEVLIAEEDYKPVGFAVYFHNFSTFKGKAGLYLEDLFVLPEHRGKGYGKKLFL
                     YLIQIARERGCERFDWSVLKWNEPSIKFYKSFGAEPLEDWVTFRLTKF"
BASE COUNT         3497 a         2503 c         1931 g         3780 t
ORIGIN      
        1 agatcagata ctcatcaaga agtttctttt gaggaaatgt gaccccgtat attctagtca
       61 gcattttatt tttttcgttg cctctccagt aagcaccggc aatagaggtg agttttacag
      121 cttttatcac tgatgtgtct acaagatgag gccctctgca taaatctgta aaggaaccat
      181 ttgtataaaa ggttatagta ccatcttcca gatcgcgtat taattcacat ttatattgat
      241 ctcccttgtc ggaatatgtc ttcatggcct cttccttgct cacttctctt ctgacaaaat
      301 gctgtttttc tttagccaac tcaagcattt tgtcttcaat tctctttaag tctgattcag
      361 ctataactct gtcccccaga tcaatatcat aataaaaacc tatttcgata gcaggaccta
      421 tgccaaattt aactcccgga taaagaagct caagcgcttc ggccataaga tgcgaagatg
      481 agtgccaaaa tgttctctta gcttcttcgt cttcccactt atgcaacttg atggcagcgt
      541 cttcatttat tggcctgtta agttcacaaa ttttgccatt aaccgttgca acaagaacct
      601 cctgagctaa ttttggactg atactttgag caatctcata aggggtaatt cccttttcgt
      661 attgacggac gctcccgtcc gggaaagtaa ttttgatcat aatctttaac acaattctta
      721 taggttgcga agttagttat tttttgttaa tccataaatt ttgcattcat aacattttta
      781 gttacttttg aatagcttaa attttacagt ttacattata aatcaacttg ctatgcaatc
      841 ttctcaatgg agaacagctg ccataaatgc gctactcctt tcaattatta ctattgttgt
      901 cagccttatc caggttgttt tcaaacccgg aacagttctc tccatactat tgtggataat
      961 aaaatttata ggatgccttg cactcttata ttatttcatg aaagagtttg gcaaagaact
     1021 tgaaagtttc tcgtataaag acggttttaa ctatggattc aaaatttgcc ttctttcgtc
     1081 aatagttctg gctgcattta actttctcca gtatgctatt ctattccctg aatctttgca
     1141 ggatgccgtt gaacaggtag caatggccat gcagtcagcc aaccctgccg gagcagaagc
     1201 catagaaaga tatcaagcaa aacttcctca aataattact gttacatcat ttttctatta
     1261 taccgttttc ggattgattg cttctgcaat tattgcaaat tatactaaaa aagaaacctt
     1321 tccaactgac aactatcagc aaacattata atttataatg gatctctcga taattatatc
     1381 cgtatacaac gaagaggaat ctcttgtaga acttatacca tggattgaga aagcccttta
     1441 tggcaaacgt attgaatatg aaataataat aatcgacgat ggaagtactg attcttcatg
     1501 gaaagttatc gaagatcttt caagaaaata tccctcattg attcgaggag tcagctttag
     1561 aagaaactat ggcaaatcgg ctgcactttt ctgtggattt gaagccgcaa ggggagatgt
     1621 tgttgtaact atggacgcag atcttcagga tagtcccgaa gagattcctg cactcttcga
     1681 tatgattaat tcagggcaat atgatcttgt ctcaggctgg aaaaagaaga gacgggacaa
     1741 ttttatagcc aaaaatattc catcaaaaat ttataatgcc actgcaagaa tggtcaccgg
     1801 cattaaactg catgacatga actgcggcct caaggcatac agaaaagagg ttgttaaaaa
     1861 tatcgaagtc tatggagaaa tgcacagata tattccttac atagctaaaa attcaggatt
     1921 cacaagaatc ggagaaaaag ttgtgattca cagacccagg aagttcggaa aaagtaaatt
     1981 cggcgtggat agatttatga atggatttct ggatcttctt actttgtggt ttctgtcaaa
     2041 atttggaaaa aggccgatgc atttttttgg cctgatagga agtctgacat tcgttattgg
     2101 agctattacc tcaatctggc taatcgttga aaaaatctcc gcacaatcca aaggccttta
     2161 ttacagggct gttacagatc aacctttgtt ttacatatct ttggtagcac ttattgtggg
     2221 tttacaattg tttcttgcag gttttgtcgg agaacttgtt acaagaaaca gcagcgaaag
     2281 aaacaactac aaaatcaaag ctacaatata aagttcagga gcatcagttc tcctcaagat
     2341 gctcctgttt gccgggaagt tctccaccgg aaagttctcc aaaagtgttt tcgtctatca
     2401 ttttacagga accattaaaa agaccaccat cttcaataaa aattcgctgg caaaatagat
     2461 tacctgttat cgaaccactt ttcttcaagc caaatgtctc tctgactact actttaccct
     2521 gaagtgttcc ccaaatgtca caacttctac ttataacatt gccgataagt ttaccggatt
     2581 cacctatcac aagcttgcct gtggtattaa gatctccttc aaaatatcca tcaattctta
     2641 tgtcacatga ggaaattaac tttccacgaa actctgttcc cacagagatt ctgttgacct
     2701 cgtttacctg tgtaactgat tccaccttag acatattgta aattattaat aattataaat
     2761 gaattacctc tccgtgagca gcagctgcag cttccataat agcctcgctc atagttggat
     2821 gaggatgaat tgaatgtgct atatctcttg ccttaacccc cagtgatctt gcgacgacaa
     2881 tgccggaaat catctctgta acgttagcac cgataagatg agcgcccaat atattatcac
     2941 tttctgcatc aataataagc tttacaaaac catctttttc tcctgatgag gccgccttgc
     3001 ctgaggctgt aaaaaaaaat ttccctactt tatagttaat tccggattcc ttgactgctt
     3061 tttcagtcat tcctacagat gctatttcgg gagaagtata ggtgcaggcg gggaaattat
     3121 tataatcgag aggtttagaa tctaatccgg caattttttc aacacagcaa atagcttcag
     3181 cagaggcgat atgagccaga gcaggagttg caattacatc tcctattgca tatataccct
     3241 tcacatttgt tgaataaaac gggtcaactc ttattttacc tctttcaacc acaacaccac
     3301 aattttccaa tcccaaattt tccgtgttgg caataacacc tactgcggat aaaactttct
     3361 ctgcttcaat atattctgta cccttctttg tcaagacttc aaccttgcat ccagaatcat
     3421 ttttctcaac actgttgact ctggcagaag taagaatttt aattcccatt ttacgaaaag
     3481 atctactcag ctgtgcagag acctcttcat cttccaaagg gacgatgttg tcaagatatt
     3541 caatcagtgt tacctctgaa ccaagtgatc tatacaaaaa tgcgaattcg cttccaatag
     3601 ctccggaacc tatcaccacc atgcttgccg gtataatttc ggggacaaga gcttgacgat
     3661 aagagattat tttttcacca tcgattggtg caaaagggag tgaattggct cttgaacctg
     3721 tagcgataac aatattagag gcctccaact gtgtggatgt tccgtcgttt aaatcaactt
     3781 ttacttttcc aggctgaaca attttaccat tcccaaaata gacagaaatt ccattttttt
     3841 tgaaaagata ctcaatacct ctgctcattc ctgaagcgac atcacggctt cttgaaacca
     3901 tcttcgaaat atcggccgat acattacagg aaatatttaa accatattcc ccggcatgct
     3961 ttgcataatt caatacttgc gcacttttta acaaagcctt ggtaggaata catccccaat
     4021 taagacaaat acctccaagc tccgctctct caaccacagc acacttcatc cccaactggg
     4081 atgcccggat tgctgctaca taaccacctg ggccggatcc aagtactata atatcataca
     4141 taagtcaaaa ttcttataaa ttagcgccat gacaattttt atactttttt ccactcccgc
     4201 atggacatgg atcatttctg ccaactttct tttcaacatg aacaggtcct tgtgattttg
     4261 gttctcctcc tgatgttgaa agctcttcgc gagatgttct aatattattt aaacctgctt
     4321 ttcttctagc ttcctgtctc agattaagtt gaggaggctg ttgggtattc tctctaagag
     4381 ggatgtgagc cttcaacaaa aaggagataa catccctgct gattttgtcg agcattgttt
     4441 taaagagttc aaaactctca aacttatata tcaaaagtgg gtctttctgt tcatatgtag
     4501 cattctgaac tgactgctta agatcatcca tatcccgcaa atgttctttc caatgttcat
     4561 ctatcatcat cagtgtaatg ctcttgttaa gagctctcac aacttcaaga ccatttgttt
     4621 catatgcttt tttgagatct actatcattt gcagcatttt atgcccatca cttacaggaa
     4681 ttgcaatatt ttgatataca gctccctgag attcataaat ttgtttgatg ataggccatg
     4741 actgctgtaa aagagtgtct ctcctgcgtt tcaggatgtc attgacatga tttagataag
     4801 cttcggtcac ttcatttaaa gaagaacctt catagaatgt ctcgtccatg ccgacttcaa
     4861 tagaaagatt tcttatcgat tcattgacaa agtcgtcaaa attggatgag ccgtgatgtg
     4921 atacaacaaa attctcaaca taatcagtaa tcatattctg aacatcaaca tcaactcttt
     4981 caccataaag agcattgcgg cggcgtgtat aaattacctc tctctgagag ttcattacat
     5041 catcatattc gagcagtctt ttacgtatcc cgaaattatt ctcctccacc ttacgctggg
     5101 ctctttctat tgattttgaa agcatactgt gctgtagcat ttctccatct ttcattccca
     5161 ttctgtctac cagacctgct atcctctccg aaccaaacaa gcgcatcaga tcatcttcaa
     5221 gagaaacata aaagctggaa gatccaggat ctccctgacg tcctgcacgg cctcttagct
     5281 gtctgtctac acgtctcgaa tcatgtctct ctgtaccaat aatagctaga cctcccgctt
     5341 ccttaacccc gggaccgagt tttatatctg tacctctacc tgccatattg gtcgcaatag
     5401 taacagatct tggttgacct gcctcagcaa caatttccgc ttctttggca tgttgctttg
     5461 cattcaaaac attatgacgt atacctctca atttcaacat tctgctaagg agttccgaaa
     5521 cctccacaga tgtagttcct accaaaaccg gtctgttcaa gtttgtgagt ctgactattt
     5581 cctctataac tgcattgtat ttttcacgtt tggtcttgaa gatcaaatct tccatatcct
     5641 ttcttatgac cggcctgttg gtcggaataa ccaccacatc taatttgtag attgaccaga
     5701 attcgccggc ctctgtctct gcagttcctg tcattccagc caatttatga tacattctaa
     5761 aatagttttg cagagtaatt gtggcaaaag tctgggtagc agcctctacc ttaacattct
     5821 cttttgcttc tatcgcctga tgaagaccat cagaatatct tctaccttcg agtatacgac
     5881 ctgtctgctc gtcaacgatt ttaatcttat tatcaagaat gacatattca acatcttttt
     5941 caaacattgt gtatgcttta agaagttgac tcacagtgtg aactctctca ctcttaaggg
     6001 catagtctgc aatcaaagca tctttggccg cagccttttc ttcatctgaa atgttttttt
     6061 tctccagctc agagatttcg cttccaatat cgggtaaaat gaaaaatttc ggatcatcta
     6121 caatcttgga aacaaattca ttacctgcgt cagtgaaatc cacagatttt tgtttttcat
     6181 ctattacaaa atagagagga tctgtcacta tatgcatctg tttattatta tcctgcatat
     6241 agaaattttc agtcttcaaa agaagctgtt taattcccgg ttcacttaaa tatttaatca
     6301 ggggattgta tcttggcaaa cccttatgag ccctcaaaag gagtaagccg ccttcctcat
     6361 cgtttccctc agatattttt tttctggctt cattaagtac ctgtgttact atagaacgtt
     6421 gagcagcata aagtttttca actataggct tatattccac aaactgctga tcatcacctc
     6481 ttggcacagg acctgatata atcaatggtg ttcttgcatc atcaattaac actgagtcca
     6541 cctcatcaac tatagcataa tggtgcttgc gctgaactat atcatcctgg tttactgcca
     6601 tgttatcacg aaggtaatca aagccaaatt cattatttgt tccaaaagta atatcggctc
     6661 tgtatgctgc ttttcttgag tcggaatttg gttcgtgctt gtcaatacaa tcaaccttta
     6721 gtccgtgaaa ttgataaaga ggccccatcc attccgagtc tctttttgaa agataatcat
     6781 tcacagtaac aatatgtact cctttacctg ccaaagcatt gaggaataca ggaagggttg
     6841 cgacgagtgt tttaccttcg ccggttgcca tctccgcaat cttgcccagg tgaagagcta
     6901 caccaccaac caactgaaca tcgtaatgaa ccatgtccca ttgaattctg ttgccaccag
     6961 ccatccaggt gtttttccaa atagctttat cttcttctat tcttacaaaa tcatgttctg
     7021 cagataaatc tttgtcactt tgagtagctg taacaacaat ctcattattc tccttaaatc
     7081 ttcgagcagt agatttcatt attgcaaatg catcaggtag aacctgaagc aaaacctctt
     7141 ccaacttctc atcaatcttt ttagtcaact tgtcaacttc tgtagcaagt gattcctttt
     7201 cagatatatc tatggtaagg tcctccagtt ttttccttaa ggattttttc tttgcctcgt
     7261 cctctgcaac atagtctgta attatctttt ttatcttttg tgtctcagct cttaactcat
     7321 catgagtaag gctgtctatc ctttcaaaag cttctttagt cttgtcaaca taaggctgga
     7381 taagcttcat gtccttttca gacttggtcc cgaagaattt tttaaggata ccgtttatga
     7441 atgccattgt ttattaaaaa ttattgatgt aatttacaaa tataagtaaa atcgaggtaa
     7501 attatttttt tatagtttac ctcgatgata atgtaattct aaacattttc ggccaatatt
     7561 tgcccgtaat atatattttg tctgtcaatg gattatatgc tatcccatta agcacgtctg
     7621 tttttgatgt tctcatactt gtcggaagga tgtttttaca atccacaact ttcctaactg
     7681 caccagtaga aggatctatt atcaatataa aatcctcctg atatacatta gcccagattt
     7741 ctccctttat atattccaat tcatttaact ggtcaacagg tttaccttca agctttacgg
     7801 caatccttcg cttttcctta aaagtggcag gatccataaa aaaaaggttt gatgaaccat
     7861 cgctcattat cagatcctta ccatcagttg taagccccca accctcctca cgaggattaa
     7921 aaaattgttt gatcggttta aacgtgttaa tatcatacac aaaaacaact ctttgctgcc
     7981 aagtaagaat atataacaaa tctcccaagg ccgttgctcc ttcagcaaaa tattcatcag
     8041 gaaaattaaa actttgcaaa gctgttcccc tggaaagatc tactttacga aaaaaagatg
     8101 caccatactg tccggaactc tcataaagtt gacctttatg aaaaaaaaga ccttgtgtat
     8161 acgaattaac atcgtgtgga agtacttcaa caattttaca tctgcatact tccgccttga
     8221 caaccatttg ggctaaggat actctatcca atacaccggc aaacaatagc actgctgtta
     8281 taaaaatttt aaaaagactc tgtgaacgcg agatttttat gatcatatgt ttgaactatc
     8341 attcttgtgt ttatgcttca acgctgcttt aacaaaggca acaaaaatgg gctgtggtct
     8401 ttcaggagta ctctttaact cagggtgaaa ctgtactccg ataaagaaag gatgtccagg
     8461 taattctaca atctcaacca gtccggtatc cggattcttg ccggatgcat gaagccctcc
     8521 attctcaaga gcttctacat aattgctatt aacctcgtat ctgtgtctgt gtctttccga
     8581 aatttccttt ttgccataaa tggaatacgc gagagagcct tcttcaatat cacacctata
     8641 tgcaccaagc ctcattgttc cacctttgat tgtaagactt ttctgatctt ccatcaaatc
     8701 tattaccgga tttttggcat tctgcttaat ttctgtagtc atggcatctt ctagtccaag
     8761 tacattttga gcatattcaa ctactgccat ttgcatgcca agacatatgc caaaaaatgg
     8821 gattccctgt tctctcgcat attttattga aatgatttta ccttggatgc ctctctcccc
     8881 aaaacctgga gcgacaagaa taccatgcat cgcggcaagt ttctcagtca cattgttctc
     8941 gttaacgtat tcactatgaa cataatgcac atttaccttg cactcatttt ctgctccggc
     9001 gtgtataaag gattccaaaa tagacttgta ggcatcctgt aattcaacat atttaccaac
     9061 tattgcaata tctactgtat ctttaggatt atgaattctg tccaaaaatt ctttccattt
     9121 tacaagttca agattgcgtg ttaagtacga atccaattta aactttctaa gaacgagctc
     9181 atccagtttt tccttataca tattcaaagg cacttcataa atactgggtg catcaattga
     9241 ctcaattaca gcatttggtc taacattaca gaacaaagca acttttcttc taatctctgc
     9301 tggtaatgga tgttctgtgc gacaaacaat tatatcgggg tgcaccccgt tttgctgtaa
     9361 agctctaact gaatgctgtg ttggctttgt ttttagctct ttggctgcat ttaaataagg
     9421 gacaagagta aggtgtacta caagacaatc ctcatcagga agttcccatt gaagttgtct
     9481 aacagcttca ataaaaggca acgactcaat atcacccact gtaccaccaa gttctgtaat
     9541 aattacgtca aaattacccg acttgccaag caacaacatt ctgcgtttta tttcatcagt
     9601 aatatgagga attatctgaa ctgtctttcc aaggtaagcg ccctgtctct ccttgtcaat
     9661 tacagttcta tagatcttgc cggtcgtaac attattggcc ttagatgtaa atgtgttcag
     9721 gaacctttca taatgaccca gatctaaatc ggtctccgcg ccatcctctg taacgtaaca
     9781 ttctccatgc tcatatggat tcaacgtacc agggtcgaca ttgatatatg ggtcaaattt
     9841 ttgaattgta acagacaacc ctcttgcttg taacaactta gctaaagagg atgcaataat
     9901 accttttcct aaggaagagg taactcctcc cataacaaaa acatacttag tgctcataaa
     9961 tacggattgt aatacagggt tgcaaagtta atctaaattt attgtttaga caattaatct
    10021 ctaattttgc ttttttgaat tactatgaaa atctacagtg aaaaatatct tgtaaaaagt
    10081 tatgagactg acatgaatgc aaaccttaaa ctatttgcat ttatgaatta tgcacaagaa
    10141 attgcaggtc agcatgcgaa tttattgggg tttggatatg acacacttat aaaagaggga
    10201 gttgtttggg tgctttcgag aatgcatatc atattcaatg atattccaaa atggaaggat
    10261 aatatcacca tgtatacatg gcacaaagga ggagacagat tattctggta tagagacttt
    10321 gttgtaaata atgaaattga agagactgtt atcaaagcga cctcttcatg ggtaataatc
    10381 aacgttgaaa caagaaaaat gcacaaaatt gaattattgg gacatcctga taccatcaac
    10441 tcagaagatg caattacaga acatgctgaa aaaattatta ctcctcatga aatggaatac
    10501 gtccacacta aaaaggtatc atatagcgat gttgacataa ataaccatac aaacaacgcc
    10561 aaatatgttg aatgggctat agattcactt gatcctgaat attattcaag catacaactc
    10621 tctgatatga ttataaattt caaccttgaa accaggctga atgatcaggt cgaaatctac
    10681 agacacgttt cagataacaa tgtgattata gagggaaaaa gagagaaaaa ctccattttt
    10741 accattgaga tgataatcaa atgatatgta caagtttata aaatcagttt tctggttaca
    10801 taatcttcat aactgatcaa ctttggatga tagtcagtat caaaaagagg agaagaaatc
    10861 aaaaaatctg ccgtagaacg gttagtagcc gtagggacat tgtagagact tgaaattctg
    10921 agtagggctt taacatcaac atcatgtggt tgaggctgca ttggatccca taagaaaaag
    10981 agtaaatcaa tctcttcctg ggcaataagg gcgcccaact gttgatcacc ccccaaagga
    11041 ccggacttga gaatagtaat atccagctcc ttaccttcat tatgtgttcc tttactgaga
    11101 gtttccctga ccattcttcc agttgtcccg gtacaaacca atttatgctt cttaagtgtc
    11161 tccttattaa actcaaccca ttccattaaa tctttttttc tgttgtcgtg tgctactaat
    11221 gcaatagtct tcataattga tgattaaaaa ttaaaatttt gtaagcctaa atgtaaccca
    11281 gtcttcgagc ggttctgctc caaaactttt ataaaacttt attgagggtt cattccattt
    11341 aagaactgac cagtcaaacc tttcgcaccc tctttccctg gcaatttgaa taagatataa
    11401 aaacagtttt ttaccataac ctttgcctct atgttctggc aaaacaaaca gatcttcaag
    11461 ataaagacca gccttccctt taaaggtaga aaaattgtga aagtaaacgg caaatccgac
    11521 cggtttgtaa tcctcctcag caatgagcac ctctgctgac ttctttacaa aaagtgaatc
    11581 atacaatatc tcctcagtag cagtcacatc atgcttcaat ttttcatatt ctgctagttg
    11641 ataaataaat ccaagaataa tgcctgtatc atctggctta gctgccctta ttataaattc
    11701 cataaatact a
//
LOCUS       DAOTQL010000043        11641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5070_length_11641_cov_5.914120, whole genome shotgun sequence.
ACCESSION   DAOTQL010000043 DAOTQL010000000
VERSION     DAOTQL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5070_length_11641_cov_5.914120"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="P5293_03930"
     CDS             <1..800
                     /locus_tag="P5293_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49084.1"
                     /translation="PFYYKSDDFIVDKNHFNLSLTFRPKIRISHTLEVEYDQTDIAKS
                     ILEENPDYWGGTDTLRRGISICYKYSSDQRDNNQYPLQGYLISGGLSCYTAFDDNFRY
                     GQLSTTLQYCYPICKRWFFSTTLSAALSAKNKNAYIFDRAIGYEMASIRGMELYVADG
                     QHYVVLNPTIKYNIIPTKVVVLDWLSFLKKFYKIHFALYGKAFFDGGYSYNRYKTDHN
                     DLSNKLLYSTGIGLDFVTYYDINISIEYSFNMLNKGGFFFSFKGPLI"
     gene            892..3297
                     /gene="lon"
                     /locus_tag="P5293_03935"
     CDS             892..3297
                     /gene="lon"
                     /locus_tag="P5293_03935"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRR49085.1"
                     /translation="MKLENIYLQQSIPEDVNIIPVVNIEGIRHIDDSELPDTLPVIAL
                     RNAVLFPETVIPITVGREKSVKLIREVYGKMKILGAVSQKDAKLEDPSPSDLFDIGTL
                     AKIIKIVEMPDGSITIILQGIKRFKIKEIVTTEPYFVASVKYLEDYIEKKNQKEFDAI
                     VDSVKDAALNIIKLSPNIPQEAAFAIKNIEGQSFLINFIASSMEVENPLDKIALLREN
                     RLKQRALKLLELLNKQIDILKIKDDIQQKVRSEIDQQQREYYLSNQLKTIQEELGINN
                     SIKEFNDLRQQASKKKWSKSVAETFEKELQKLERTNTGSPDFNIQLSYVKFLLELPWE
                     EFSSDNLDLKHAQKILDDDHYGLETVKDRIIEHLAVLKLKGDMKSPILCLYGPPGVGK
                     TSLGKSIAKALGRSYERISLGGLHDEAEIRGHRRTYIGAMPGRIINSIKKAGTSNPLL
                     VLDEIDKVSNDFRGDPASALLEVLDPEQNVAFHDNYLDIDYDLSKVLFITTANNITTI
                     HPALRDRMELINVSGYLAEEKREIAKGYLVPRQIEAHGLKKSQFKISIDGIDTIINEY
                     TRESGVRGLDKQIAKLARITAKNIALDLPYNSLLGPKEVKGMLGVPIYQHETQKGNEA
                     SGVVTGLAWTENGGEILFIECVMSKGKGALSTTGNLGDVMKESAIIAYQYLKANPQLL
                     SLSPKDFSDRDIHIHVPEGSIPKDGPSAGITMISAMASAFLKKKVKSGIAMTGEITLR
                     GRILPVGGIKEKILAAKRAGIDTIILSEDNRKDIEEIKEIYLKGLNFKYVKTIPEVLE
                     FIF"
     gene            3300..3962
                     /gene="ung"
                     /locus_tag="P5293_03940"
     CDS             3300..3962
                     /gene="ung"
                     /locus_tag="P5293_03940"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRR49086.1"
                     /translation="MDVKVEESWKRELQEEFDKDYFREITAFIKRDIASNIIIYPKGS
                     LIFNAFNSTPFDKVKVVLLGQDPYYNPGQAHGLSFSVPDGVPFPPSLVNIFKELNADL
                     GIEPPTNGSLQRWAQQGVFLLNAVLTVRAGLPASHSKIGWTKFTDAVIKKISDKKEGI
                     VFLLWGNYARSKKTLIDTKRHYVLEAAHPSPLAKGAFFGCRHFSKTNEILLKQGKTPI
                     IW"
     gene            complement(3952..4623)
                     /locus_tag="P5293_03945"
     CDS             complement(3952..4623)
                     /locus_tag="P5293_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRR49087.1"
                     /translation="MTISERILNIKNELPPSVKLVAVSKFKPVEDIMEAYNAGLRDFG
                     ENRPQEMAEKIKLLPKDIKWHFIGHLQTNKIKLILGDVFMIHSVDSLKLSREINRQAY
                     SRGLVKDCLLQVHIAREETKQGFLIEELNCLIEELLSLENIRICGLMGMASFVNDITL
                     IRGEFRNLKEQFDYLKKHYFTKNDCFKEISMGMSSDYKIAIEEGSTIIRIGTGIFGSR
                     LQSTK"
     gene            complement(4620..6491)
                     /gene="yidC"
                     /locus_tag="P5293_03950"
     CDS             complement(4620..6491)
                     /gene="yidC"
                     /locus_tag="P5293_03950"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRR49088.1"
                     /translation="MKKNSTIGFLIIGAILIGFSWYNSKIYNEQLKKQAQIDSIARVK
                     AQEVLEQEALDSASKITSFKDSIGIVPNNASQSIYKDSLLIMANEASEEFFTLENDKI
                     KITFINRGAQPYKVEIKNYYTFDSLPLFLVKDSASSLSLVLFTNQEIKTDAFKFNRAE
                     SSDSSVIYRLPFDEDSFIEYTYNLSAGSYLVDFNIRLVGMQEHIQKKVTTMDLNWVMD
                     VPRLEKGYDNEKNYSSVLYKHPNAKSVESLGLRKQNAEKDVRTKINWVAFQQQFFSAI
                     LVSKDNFNMGFLSYKIYPEEDPFKNLMHCEANMQIPCSYGEEINLPFQLYYGPNHYKT
                     LKSYGQGFEKIVPLGGWLIGWINRFIVIPVFDFLSKFISNYGIIILILTLIIKIIISP
                     LTLRSYMSTAKMRVLKPEIDKINEKYPRKEDALKKQQETMALYKKAGVSMFGGCIPVL
                     LQMPILFAMFKFFPASFELRQQGFLWAEDLSGYDSILNLPFRIPLYGDHVSLFALLMA
                     LSTYFYSKMNADQMGSGPQMAGMKFMSLYFMPIFLLVLCNNFSSGLSYYYMLSNVLTM
                     LQTWIIRKFFVDEKKLYAKLKEKASSNAPVKKSKFQQRLDEAYRMQQQQLQQQKKRK"
     gene            complement(6492..8327)
                     /locus_tag="P5293_03955"
     CDS             complement(6492..8327)
                     /locus_tag="P5293_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HRR49089.1"
                     /translation="MDQDNIPTASQEQFKEKDILENTMSKDSDSEYPDHSNKSLKEIL
                     QIFQEMIERGDQQELYKHAEGIKAAFYKALKREKIAAGFIEPSENNVVNEDDQTESNI
                     SLNPFAEVERGFKELFNRYKTLRSDFLQTIEKQKEENLAKKLEIIEELKELLEKQEDL
                     HHTFPAFRELQNRWKSIGPVPQSNSKNVWETYQYLVEKFYDFVKINNELRDLDLKKNL
                     EMKVELCEKAEALSEEPNAVLAFKKLQKLHEEWREIGPVSKEMREEIWDRFKKATAVI
                     NKNQQQFFENQKDIQKQNLELKKALCERVEAIVAMEGEKDWNALTKEIENIQKEWKGI
                     GFASKKDNQKIYERFRSACDQFYNAKREHYSNFKSVMQENYEKKIALCEQAEALKDST
                     DWKRTTDQLISIQKKWKEIGPVARKQSDAVWKRFRAACDEFFENKSKYYNSVDDSFEQ
                     NLAKKLELIKEITDYKLDTKELENREALKDFQARWAEIGFVPFKDKERIQAAYRYALE
                     QKFGDIRNLDQENKLSRYKKHIKEVQTFSKGGGLRTERERLIQKYRQMEQDIAILENN
                     IGFFTKSKNADSLLLEIDKKISAAKYELTQLEEKIKLIDNQKEQQ"
     gene            8406..9086
                     /gene="trmD"
                     /locus_tag="P5293_03960"
     CDS             8406..9086
                     /gene="trmD"
                     /locus_tag="P5293_03960"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRR49090.1"
                     /translation="MIRIDILSVVPELLESPLNHSIVQRAKSSGLAEIHIHNIREYGK
                     GNYRQVDDYCFGGDAGMVLMIEPVFKIITKLKDERNYDEIIYMSPDGELFNQKIANQL
                     SCMNNIILLCGHYKGIDHRIREHLITREISIGDYVLSGGEIPAAIICDAVIRLIPGAL
                     SDESSALSDSFQDNLLAPPVYTRPAEFNGWKVPDVLLSGNPKLIKDWQEQESIERTKK
                     LRPELLKD"
     gene            complement(9083..10615)
                     /locus_tag="P5293_03965"
     CDS             complement(9083..10615)
                     /locus_tag="P5293_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR49091.1"
                     /translation="MYKTLKWIRVALSLLILIILTLNFFYFSNTSPNFSAVLLKFQFV
                     PSMLGILTGSALFFIILIILTFLFGRVYCSTLCPVGTFQDIVSRFASLFKSKKNRRYK
                     YSKGYDWLRYSVLVLVMLPLIGGITLPIAILDPYSNWGRISSQLFDRGVQTINNIIAS
                     IFPNTFYINNYSHFALAALLAAIVFLLVIICFSAIRGRLYCNTICPVGSILGGLSKFA
                     LFKPEINGNCNQCMLCVSKCKSQCIDVKNHKIDVSRCVACLDCMQGCNRNAIGYRFAF
                     KKVENKSSEEGKNIDKQGRRRAILTLGLLSGVVAARAAKIGPLVRTKAEKNAIAPPGA
                     VSFENLKNHCTTCHACISACPSQIIKPALLEYGLEGIMMPTLSYKDHFCTYECNKCSQ
                     VCPNGALLPLSIEEKKLCQIGKAKYCLKECIVYRDGTDCGACDEHCPTNAITMVPYKD
                     TGLYIPKLDRNVCIGCGACEYICPASPVKAIIVIGNEEHQIAMEAAKEEKKEVKIDEF
                     GF"
     gene            complement(10627..11556)
                     /locus_tag="P5293_03970"
     CDS             complement(10627..11556)
                     /locus_tag="P5293_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRR49092.1"
                     /translation="MERRGFLKVAAASSLLGFLGGGSSILRASEPASPPNINDLVAVM
                     GGEPEAMYLRAIKEMGGISRFVKKGQRVVVKPNIGWNKKPEEAANTNPQLVAAIVKDC
                     LKAGASEVVVFDHTCDSWMPCYKNSGIEAAAKAAGARIAFGHDEKYYRMVNIPRGVRM
                     KQAKIHEEILDCDVWINVPVLKNHGGARMTISMKNFMGIVWDRKFLHANDLQQCIADC
                     STISKMPVLNIVDAYRIMTTNGPVGKGPEDVQLAKALFMSTDIVAADTAALKFFSQFR
                     EMKLSDVGHIMIAEKMKIGTTDLDKLQVSRIKM"
BASE COUNT         3753 a         2196 c         1930 g         3762 t
ORIGIN      
        1 ctccatttta ttataaatcc gatgatttta ttgttgacaa aaatcatttt aacctttccc
       61 tgacatttcg tcccaaaata agaataagtc ataccttaga agtagaatac gatcaaactg
      121 atatagctaa aagtatactt gaagaaaatc ctgattattg gggaggaact gatacattga
      181 gaagaggaat atcaatctgt tataagtata gttcagatca aagagacaat aatcaatatc
      241 cactacaagg atatctaata tctggcggat tgagctgtta tacagcattt gatgacaatt
      301 tcagatatgg acagttgtca acaacactac aatactgtta tccgatttgt aaaagatggt
      361 ttttttcaac gacactctct gcagctttat ctgcaaaaaa taaaaatgca tatatctttg
      421 atagagcaat tggttatgag atggcgtcaa taagggggat ggagctttac gtagcagacg
      481 gtcaacatta tgtagtgctt aatcccacta ttaaatacaa tataatccca acaaaagtag
      541 tcgttttaga ctggttatct tttttaaaga aattttataa gatacacttt gcactttatg
      601 gcaaagcttt ttttgatgga ggatattcat ataatcgata caaaacggat cataacgatt
      661 tatctaacaa gttgttgtat agtacaggta ttggccttga ttttgtaaca tattatgata
      721 ttaacatatc tattgaatac tcgttcaata tgcttaataa gggtggcttc ttcttttctt
      781 tcaagggtcc attaatttag tttggcacaa tcattgacct tgaggaaatg aacaaataat
      841 agcgttattc gttgcttata agtgtgacaa attggcataa cttaaatgat tatgaagtta
      901 gaaaatatat atttacagca atctattcca gaagatgtta acataatccc tgttgttaat
      961 atcgaaggta taaggcatat tgatgattct gaactgcctg ataccttacc tgtcattgcc
     1021 ttgagaaatg ctgttctttt ccctgaaaca gtaatcccta taacggtagg gagagaaaaa
     1081 tccgttaaac ttattagaga ggtctacggg aaaatgaaaa ttcttggagc tgtctcacaa
     1141 aaagacgcaa aacttgaaga tccttcaccg tcagaccttt ttgatatagg aactttggca
     1201 aagattatca agatagttga aatgcctgat ggaagcatta ctattatttt acaaggcatc
     1261 aaaagattca aaatcaagga gattgttaca acagagcctt actttgtcgc ctctgtaaaa
     1321 tacctcgaag attatattga aaaaaagaac cagaaagaat ttgatgctat tgtagactct
     1381 gttaaggatg ctgccttaaa cattattaag ctttctccta atattcccca ggaggctgca
     1441 tttgctatta aaaatattga aggacaatca ttcttgatca attttattgc ttcaagcatg
     1501 gaggttgaaa atccacttga caaaattgct ctattaagag aaaatagact taaacaaaga
     1561 gcattaaagc ttcttgaact tttaaacaag caaattgata tactgaaaat taaagatgat
     1621 attcagcaga aagttagaag tgaaattgat cagcagcaaa gagagtacta tttgagtaat
     1681 cagctcaaaa ctatacagga agaacttgga attaataaca gtataaaaga attcaatgat
     1741 ctgaggcaac aagcttcaaa gaaaaaatgg tctaaaagtg ttgccgaaac ttttgaaaaa
     1801 gaacttcaaa aacttgaacg gaccaatacg ggttctcctg atttcaatat acaactttct
     1861 tatgtcaaat tcttacttga actgccatgg gaagaattca gttctgacaa tctggacctg
     1921 aaacatgcgc aaaaaatact ggatgatgat cattatgggc ttgaaactgt aaaagacagg
     1981 ataatagagc atttggcagt gttgaaatta aagggtgaca tgaaatctcc tattttatgc
     2041 ctttatggtc ctccaggcgt gggtaagaca tctttgggca aatcaatagc aaaagcacta
     2101 ggaagaagtt acgaaagaat ttcacttggc ggattgcacg acgaggccga aataagagga
     2161 cacagaagaa catatattgg agcgatgcct ggcaggataa ttaattcaat aaaaaaagcc
     2221 gggacatcca atcctttgct ggtgcttgac gaaatagata aagtcagcaa cgatttcaga
     2281 ggagatcctg cttctgctct tctagaagtc ctcgatccag agcaaaatgt tgcatttcac
     2341 gataattatc ttgatattga ctacgacctt tcaaaggttc tatttattac tacagcaaat
     2401 aatatcacaa caattcatcc tgctttaaga gacaggatgg agttaattaa tgtcagtggt
     2461 tatcttgcag aagaaaaaag agaaattgca aaaggttatt tagtcccaag acagatagag
     2521 gctcacggtc tcaagaaaag tcaattcaag attagtatag acggaattga cactataatt
     2581 aatgaataca caagagaatc cggagtcaga ggtcttgaca aacaaattgc aaaacttgcc
     2641 agaattactg ctaaaaatat tgctcttgat cttccatata attctcttct tgggcctaaa
     2701 gaggtaaagg gaatgcttgg agtacctatt tatcaacatg agacacaaaa gggaaatgaa
     2761 gcttcgggtg ttgtcacagg actcgcatgg actgaaaatg gaggggaaat cctttttata
     2821 gaatgtgtaa tgagcaaagg taaaggagct ctatcaacta caggtaatct tggtgatgtt
     2881 atgaaagaat ctgcaataat tgcatatcaa tatttaaaag caaatcccca attgctttct
     2941 ctcagcccta aagattttag tgacagagat attcatattc acgtgccaga aggctccatt
     3001 ccaaaagacg gtccatctgc aggaataaca atgataagtg ccatggcctc cgcttttctt
     3061 aagaaaaaag ttaaaagtgg cattgcgatg acgggagaga tcacattgag aggaagaatt
     3121 ttacctgttg gaggaattaa ggagaaaatc ctggcagcca aaagggcagg aattgacaca
     3181 atcattctat ccgaagataa tagaaaagat atagaagaaa ttaaagagat ttaccttaaa
     3241 gggcttaatt tcaagtatgt taaaactata cccgaagtcc ttgaatttat attctaaaaa
     3301 tggatgtaaa agttgaagag agttggaagc gtgaactcca ggaagaattt gacaaagatt
     3361 actttcgaga aattactgct tttatcaaaa gagatatcgc ctctaatatt atcatatacc
     3421 caaaaggcag tttgattttc aacgctttca actctactcc ttttgataag gttaaggttg
     3481 tcttacttgg acaagaccca tactacaacc ctggtcaagc tcacggtttg tctttttcag
     3541 taccggatgg ggttcctttt cccccttctc ttgttaatat tttcaaagaa cttaatgctg
     3601 acttggggat agagcctcca acaaatggtt ctttacagcg atgggcacaa cagggagttt
     3661 ttttgttaaa tgctgtcctt acagtaagag cagggttacc tgcatcccat agcaaaattg
     3721 gatggactaa atttactgat gctgtaatca aaaaaatttc tgataaaaaa gagggaatag
     3781 tgttcctttt atggggtaat tacgcaagaa gtaaaaaaac attaattgac accaagcgtc
     3841 attacgttct tgaagcggca catccgtctc cccttgccaa aggtgctttt tttggatgca
     3901 ggcacttttc aaaaacaaat gaaattcttc tcaaacaagg taaaacacca attatttggt
     3961 agattgtaaa cgactcccaa agatccctgt tccaattcgt ataatcgtac tcccctcttc
     4021 aattgcaatt ttgtaatcac tggacatacc cattgaaatt tctttgaaac aatcgttttt
     4081 agtaaaataa tgttttttaa gataatcgaa ttgttcttta agatttcgaa actctcctct
     4141 tataagagtt atgtcattta caaaagaggc cattcccatc agtccgcaaa ttctgatatt
     4201 ttcaagagat aacagctcct caattaaaca gttaagctct tctatcaaaa atccctgttt
     4261 cgtctcttct cttgcgatat gaacctgtag aagacaatct ttaaccaacc cccttgaata
     4321 ggcttgacga ttaatttccc gggaaagttt taaagaatct actgaatgaa tcatgaatac
     4381 gtcacccaga atcagtttaa tcttattagt ctgaaggtga cctataaaat gccacttaat
     4441 gtcttttgga agcagtttta ttttttcagc catttcttgt ggacgattct caccaaaatc
     4501 tcttaaacct gcattatacg cctccattat atcttctaca ggtttaaatt ttgaaacagc
     4561 caccaattta acagaaggtg gtaattcgtt tttaatattc aatattcttt cggaaatagt
     4621 catttccttt tcttctgctg ttgaagctgt tgttgttgca tcctataagc ttcatcaagc
     4681 ctttgctgga attttgactt tttaacaggt gcatttgatg aagccttttc cttaagcttg
     4741 gcatataatt tcttttcatc cacgaaaaac tttcttatta tccacgtctg aagcattgtc
     4801 agaacatttg aaagcatgta ataataactc aaaccacttg agaaattatt acagaggaca
     4861 agaagaaata tcggcataaa atagagactc ataaatttca tacccgccat ttgtggacca
     4921 gagcccattt gatctgcatt catcttggaa taaaaatatg ttgaaagagc cattagcagt
     4981 gcaaaaaggc tcacgtgatc accatagaga ggtattctaa agggtaaatt gagaatagaa
     5041 tcgtaaccac ttaaatcttc tgcccaaaga aaaccttgct gcctcaattc aaatgatgcg
     5101 ggaaagaact taaacatggc aaacagaata ggcatctgca ataagacagg gatacaacct
     5161 ccaaacatgc tgacacccgc ctttttatat aacgccatag tttcctgttg ctttttaagt
     5221 gcatcttctt tcctgggata tttttcatta atcttatcaa tttctggctt taaaacacgc
     5281 atttttgcgg ttgacatata agaccttaat gtaagaggag aaattattat tttgataatt
     5341 aaagtgagaa ttaaaataat aataccataa ttacttataa acttgcttag aaagtcaaaa
     5401 acaggaatga caataaatct attaatccaa cctatcagcc agcctcccag tggaacgatt
     5461 ttttcaaagc cttgaccata tgatttaagg gttttgtagt ggttaggacc ataataaagc
     5521 tgaaaaggaa gattgatttc ttctccatag gaacacggaa tctgcatatt ggcttcacaa
     5581 tgcatcaagt ttttaaaagg atcctcctct ggataaattt tatagctaag aaaacccatg
     5641 ttaaaattat cctttgaaac cagtattgct gaaaaaaact gctgttgaaa tgcaacccaa
     5701 ttaattttcg ttcttacatc cttctctgcg ttttgttttc ttaaccccag actttcaacg
     5761 ctttttgcat ttggatgttt ataaagtact gacgaataat ttttttcatt atcatatccc
     5821 ttttcgagtc ttggaacatc cattacccag ttaagatcca tggtcgtaac cttcttctga
     5881 atatgttcct gcatacccac aagccttata ttaaaatcta ctaaataact gccggcggat
     5941 aaattatagg tgtattctat gaaagaatct tcatcgaagg gtaatctgta aattacagaa
     6001 gaatcagatg actcagctct gttaaattta aaagcatccg tttttatctc ctgatttgta
     6061 aacagcacta aagacagtga actggcacta tctttaacca agaaaagagg aagagaatca
     6121 aaagtatagt aatttttaat ctcaacttta tagggttgag ctcccctatt gataaatgta
     6181 attttaatct tatcattttc aagggtgaaa aattcttcag aagcttcatt ggccataata
     6241 agcaaagaat ccttataaat tgactgtgaa gcattattag gaacaatacc aatagaatcc
     6301 ttgaaacttg taatctttga agctgaatca agtgcctctt gctccaacac ctcctgtgct
     6361 ttaacgcgcg ctattgaatc aatctgtgcc tgttttttta gctgttcatt gtatattttg
     6421 gaattgtacc aactaaaccc aataagtata gctccaatta ttaaaaatcc aatagtggaa
     6481 tttttcttca tctattgttg ttctttttga ttatcaatta atttaatttt ctcctcaagc
     6541 tgagtcaatt catacttggc tgcagaaatt ttcttatcta tttcgagaag taaagaatct
     6601 gcattctttg atttagtaaa gaaaccaata ttgttctcca aaatagctat atcctgttcc
     6661 atctgtctgt atttctggat cagcctttct ctttctgtcc ttaatccacc tccttttgaa
     6721 aatgtctgga cttccttgat atgtttttta taacgactga gtttattctc ctgatctaaa
     6781 ttccttatat ctccgaattt ctgctctaaa gcatatctgt atgcagcctg gatcctctct
     6841 ttatccttaa aggggacaaa acctatctca gcccatctgg cctgaaaatc tttaagcgcc
     6901 tctctatttt ccaattcctt ggtgtctaat ttataatcag tgatttcctt gatgagttcg
     6961 agtttctttg ccaaattttg ttcaaaagaa tcatcaacag aattatagta ttttgacttg
     7021 ttttcaaaaa attcgtcaca tgctgctcta aatctcttcc acacagcatc agactgtttc
     7081 cgtgccacag gacctatttc cttccacttc ttttgaatgc tgatgagctg atcagtagta
     7141 cgtttccagt cagtactgtc ctttaatgcc tctgcttgct cgcaaagagc aatctttttc
     7201 tcataattct cctgcattac acttttgaag tttgagtaat gttctcgctt tgcgttataa
     7261 aactgatcac atgcactcct gaaacgttca tagatctttt gattgtcttt cttggaagca
     7321 aaaccaattc ctttccactc tttctggata ttttcaattt cctttgttag tgcattccaa
     7381 tccttctcgc cttccattgc aactatggct tcgactctct cgcacagagc ctttttcagt
     7441 tcaagattct gtttctgaat atccttctgg ttttcaaaaa attgttgctg atttttgttt
     7501 attactgctg ttgctttttt aaatctatcc caaatctctt ctctcatttc cttactaaca
     7561 ggacctattt ccctccattc ttcgtgcagc ttttgaagtt ttttaaaagc taacacagcg
     7621 tttggttctt cagagagtgc ttctgccttt tcacacaatt caaccttcat ttcgagattt
     7681 ttcttgagat caagatctct tagttcgttg ttaattttta caaaatcata aaatttctca
     7741 actagatact gatacgtttc ccaaacattc ttactattag actgaggtac aggtcctata
     7801 gacttccatc tgttttgtag ctctctgaaa gcaggaaatg tatgatgaag atcttcctgt
     7861 ttttcaagta gttcttttaa ttcttcaata atttcaagtt tcttagccaa attttcctct
     7921 ttttgtttct cgatagtttg gagaaaatca cttcttaaag ttttatatct gttaaataac
     7981 tctttaaaac ccctctcaac ttcagcaaaa ggattaagag agatatttga ctcagtctga
     8041 tcatcttcat ttacaacatt gttttcagaa ggctctataa agccggctgc tattttttct
     8101 cttttgagag ccttgtaaaa agcagctttt atcccttcgg catgtttata caactcctgc
     8161 tgatctccac gttcaatcat ctcctgaaat atctgcagaa tctcttttaa acttttattt
     8221 gaatgatctg gatactctga atcagagtct ttactcatcg tattttcaag gatatctttc
     8281 tccttgaatt gctcttgaga agcagtagga atattgtctt ggtccattat tgaaaggcta
     8341 aaatgttatt ttcttgcaaa gataaaaaaa aattgctaac gtcgtgattt atgtaaattt
     8401 gcaaaatgat acgtatagat atattaagtg ttgttccaga attgcttgaa agtcctttaa
     8461 atcattctat tgtacaaaga gctaagtcaa gtggtctggc agaaatccat attcacaaca
     8521 tacgtgaata cgggaaaggt aattatcgac aggtagatga ctattgtttt ggaggagatg
     8581 ccggaatggt tttgatgata gagcctgtat ttaagataat tacgaaatta aaagacgaaa
     8641 gaaattatga tgaaataatt tacatgagtc ctgatggaga actttttaat caaaagatag
     8701 caaatcaact ctcatgcatg aataatatta tattactctg cggacattac aaaggaattg
     8761 atcatcgaat acgagaacat cttattacca gagaaatatc aataggagat tatgtgctca
     8821 gcggtggaga aattccggct gcaattattt gtgatgcagt cattcgtctg attcctggtg
     8881 cactttctga cgaatcgtca gccttaagcg actcattcca ggacaattta ctcgcacctc
     8941 ctgtttatac aagaccggca gaatttaacg gatggaaagt ccctgacgtt cttctttcag
     9001 gtaatccaaa gcttatcaaa gattggcaag agcaagaatc aattgaaaga acaaaaaaac
     9061 ttcgacctga acttcttaaa gattaaaatc caaattcatc tatcttaact tccttctttt
     9121 cttcttttgc tgcctccatg gcaatttgat gttcttcgtt acctattaca atgattgctt
     9181 taacaggtga agccggacat atatactcac atgctccaca accgatgcaa acattcctgt
     9241 caagctttgg aatataaagt cctgtatcct tgtaaggtac catggtaatt gcattagttg
     9301 gacaatgttc atcgcaagct ccacaatcag ttccatctct ataaactata cactctttta
     9361 aacaatattt agctttaccg atctgacaaa gctttttctc ctcaattgaa agaggtaata
     9421 gcgctccgtt aggacaaact tgagaacatt tgttgcattc ataagtacag aaatgatctt
     9481 tgtaactgag cgtaggcatc ataatgcctt ccaggccata ttccaaaagt gctggcttaa
     9541 ttatctgact tggacatgca gatatgcatg catgacaggt tgtacaatga ttctttaagt
     9601 tttcaaaact aacagcacca ggaggcgcta tagcgttttt ttcagctttt gttctgacaa
     9661 gtggtccaat tttggctgcc cttgcagcaa ccactccact aaggagacct aatgtaagaa
     9721 ttgctcttct tcgcccttgt ttgtcaatat ttttcccttc ttcagaactt ttgttttcaa
     9781 cttttttaaa ggcaaacctg tatccgatcg catttctgtt acagccctgc atacaatcta
     9841 aacaagcgac acacctgctt acatctattt tatggttctt aacatcaata cattgacttt
     9901 tacacttaga gacacatagc atgcactgat tgcagttgcc attaatttca ggcttaaata
     9961 aggcaaattt agaaaggcca ccaagaatag accctacagg acatatcgta ttgcaataaa
    10021 gccttcctct gattgcactg aaacatatta tcaccaataa aaaaacaata gctgcaagta
    10081 atgccgcaag tgcaaaatga gaataattat ttatataaaa agtattaggg aatattgagg
    10141 caattatgtt atttatagtc tgtactcccc tatcaaacag ttgacttgaa attctccccc
    10201 aattggaata aggatccaaa atggctatcg gcagtgttat tcctcctatt aacggtagca
    10261 ttactaatac aagaacagaa tatctcaacc aatcatatcc tttagaatat ttataacggc
    10321 gattcttttt tgatttgaat aaagaagcaa atctcgagac aatgtcttga aatgtaccaa
    10381 caggacaaag agttgaacag taaactctcc caaacagaaa tgtgagaata atgagaatga
    10441 taaaaaaaag tgccgaacct gtcaaaatgc caagcattga cggcacaaac tgaaacttga
    10501 gaagtactgc tgagaaattt ggtgatgtgt ttgaaaaata gaaaaaattc agggtcagaa
    10561 ttataaggat taataaagaa agagcaactc tgatccattt aagagtttta tacattatga
    10621 tgaatattac attttaattc ttgaaacttg caatttgtcg agatcagttg tgccaatttt
    10681 catcttttcg gcaatcatta tatgccctac atcagatagt ttcatctctc taaactgact
    10741 gaaaaattta agtgctgccg tgtctgccgc aactatatct gtcgacataa aaagtgcctt
    10801 agcaagctga acatcctcag gacctttacc tacaggacca tttgtggtca taattctgta
    10861 agcatctaca atattcaata caggcatttt agaaattgtg gagcagtcgg ctatacattg
    10921 ttgcaaatca ttagcatgca agaattttct atcccacaca attcccataa agtttttcat
    10981 gctgatagtc atccttgcac ctccatgatt ctttaataca ggaacgttta tccatacatc
    11041 acaatcgaga atttcttcat gaattttagc ctgcttcatt cttacacctc taggtatatt
    11101 aaccattcta taatactttt catcatgacc gaaagcgatt cttgcaccag ctgcttttgc
    11161 agcagcttca attccactat ttttgtaaca tggcatccat gagtcacaag tatgatcaaa
    11221 taccacaact tcagaagcac ctgctttcaa acaatctttt acaattgcag ctacaagttg
    11281 aggatttgta tttgcggcct cttcaggctt tttattccag cctatatttg gtttcacgac
    11341 cactctctgt ccttttttaa caaaacggga aatacctccc atctctttta tagcccgtaa
    11401 atacattgcc tcaggctcac ctcccataac agccactaaa tcattgatat ttggtggact
    11461 tgccggttct gaagctctca aaatgcttga accacctccc aaaaaaccca ataagctact
    11521 tgctgcagca actttaagga atcctcttct ttccataaaa agataaattt tgttgcaaat
    11581 atatcaaata aatatttttt actaccttta aaaataatta aaaagtgaga taagtgctta
    11641 a
//
LOCUS       DAOTQL010000044        11492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5141_length_11492_cov_4.889919, whole genome shotgun sequence.
ACCESSION   DAOTQL010000044 DAOTQL010000000
VERSION     DAOTQL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5141_length_11492_cov_4.889919"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="thrS"
                     /locus_tag="P5293_03975"
     CDS             <1..1217
                     /gene="thrS"
                     /locus_tag="P5293_03975"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRR49093.1"
                     /translation="DHRKIGKELELFAFSQKVGQGLPLWLPKGAQLRERLENFLRIVQ
                     KKGGYLPVITPHIGQKELYVTSGHYAKYGADSFQPIHTPQEGEEFLLKPMNCPHHCEI
                     YKTKPRSYKDLPLRFAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCRQDQIKEEFN
                     KVIDIVLYIFKTLDFKDFTAQISLRDPENKEKYIGSDENWEKAERAIIESAHEKGLNT
                     VIVLGEAAFYGPKLDFMVKDAIGRKWQLGTIQVDYNLPERFDLEYIGSDDKKYRPVMI
                     HRAPFGSMERFVAVLIEHTGGKFPLWLAPDQAVILPVSEKYNEYAKKVVDLLTNNDIR
                     ALIDDRNETIGKKIRENELKRIPYILIVGEKEAEAGTVSVRRQGEGDKGTMTPDNFVE
                     VVNREIKEQIGY"
     gene            1239..1859
                     /gene="infC"
                     /locus_tag="P5293_03980"
     CDS             1239..1859
                     /gene="infC"
                     /locus_tag="P5293_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRR49094.1"
                     /translation="MESTFKPRQGVSSSRTPGSNKSGNPSREHQYRKAPVQDGPRVNN
                     EITAPKVRVVGENIQNPGIYSFSEAIKMAEALELDLVEISPNADPPVCKIVDYQKYLY
                     QQKKKAKEMKSNASKVVIKEIRFGPNTDEHDYQFKLKHAQAFLQEGCKVKAFVFFKGR
                     SIIFSDQGEKLLLRFALDLEDYGKAEQMPKLEGKRMIMMLAPIKKK"
     gene            1885..2079
                     /gene="rpmI"
                     /locus_tag="P5293_03985"
     CDS             1885..2079
                     /gene="rpmI"
                     /locus_tag="P5293_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRR49095.1"
                     /translation="MPKMKTNTGAKKRFELTGTGKVKRKHAFKSHILTKKSTKRKRNL
                     THTAIVSPADEKKVKDLLVS"
     gene            2157..2501
                     /gene="rplT"
                     /locus_tag="P5293_03990"
     CDS             2157..2501
                     /gene="rplT"
                     /locus_tag="P5293_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRR49096.1"
                     /translation="MPRSVNSVASRARRKKILKQTRGNFLARKNVWTVAKNTWEKGLT
                     YAYRDRKTKKRNFRALWIQRINAAARQHGMNYSQFMGKLAKNNVGLNRKVLADLAMND
                     PKAFEALINSIK"
     gene            2571..3857
                     /locus_tag="P5293_03995"
     CDS             2571..3857
                     /locus_tag="P5293_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRR49097.1"
                     /translation="MLWVIWLENWWFLLGLIIIFDIFITRKVKWAFWKKNYKKGEKRN
                     VWLDWLDAIIFALIASAFIKGFFFEAYMIPTSSMERTLMTGDYLFVSKVAYGPRVPMT
                     PISVPLIHNVLPITGGESYVKWIQNPYRRMAGFRSVKRDDIVVFNFPNGDTVLKGDPT
                     ADYYQCIRLYGREYTYKTYGPVVVRPDDKKDNYVKRCVATAGDTLEIINGKVYVNGLP
                     QNNYPGIQNTFTVYTNGTTINSKIIDDMDINPNELYFDVSLPGYINFPLNEDEVEKIR
                     AMANVIDVRQNIDTYPPDLPDSPLTIFPFEGDFRWTRDNFGPLWVPKKGTTVKLTIAN
                     LPLYKRIISSYEDNSLEVKDSLIFINGRVVDSYTFKMDYYFMMGDNRHNSADSRYWGF
                     VPESHVVGTPSVIWFSKDKNKSFPKNIRWKRIFKTV"
     gene            3942..4184
                     /gene="rpmB"
                     /locus_tag="P5293_04000"
     CDS             3942..4184
                     /gene="rpmB"
                     /locus_tag="P5293_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRR49098.1"
                     /translation="MARVCQITGKRRMIGNNVSHSKRRTKREFSPNLKNKRFWLEEEQ
                     RFVTLKVSTAGMRNIYKNGLAATLRKAQKNGLIDLY"
     gene            4204..4386
                     /gene="rpmG"
                     /locus_tag="P5293_04005"
     CDS             4204..4386
                     /gene="rpmG"
                     /locus_tag="P5293_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRR49099.1"
                     /translation="MAKKGNRVQVILECIEQRESGVPGISRYITTKNKKNTTQRLERK
                     KYNPYLKKVTIHREIK"
     gene            4408..4569
                     /locus_tag="P5293_04010"
     CDS             4408..4569
                     /locus_tag="P5293_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HRR49100.1"
                     /translation="MAKKVVATFGSGKGTGKNVVKCIRMVRSERTGAYTFEEEIVPTE
                     GVKDFFARK"
     gene            4692..>5205
                     /locus_tag="P5293_04015"
     CDS             4692..>5205
                     /locus_tag="P5293_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HRR49101.1"
                     /translation="MALFGLFGSKSKEEKEALDKGLEKTKKGIFEKLSRAVVGKSKVD
                     DDTLDDIEEALIASDIGVETTLKIIDRLEARVHRDKFMDSNELNQILKEEIEALLNVD
                     DNHEGDMPFDFSKKPYVILVVGVNGVGKTTTIGKLAARLKNSGYKVVLGAADTFRAAA
                     VEQLTIWSERA"
     assembly_gap    5206..5305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5306..5800
                     /locus_tag="P5293_04020"
     CDS             <5306..5800
                     /locus_tag="P5293_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HRR49102.1"
                     /translation="DTFRAAAVEQLTIWSERAGVPIVKQAMGADPASVAYDTVSSAIA
                     QGADVVLIDTAGRLHNKVNLMNELTKIRNVIKKIIPDAPHEVLLVLDGSTGQNAFQQA
                     KEFTKATDVTALAITKLDGTAKGGVVIGISDQFKIPVKFIGVGEKIEDLQIFNKKEFV
                     ESLF"
     gene            5819..8287
                     /gene="pheT"
                     /locus_tag="P5293_04025"
     CDS             5819..8287
                     /gene="pheT"
                     /locus_tag="P5293_04025"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRR49103.1"
                     /translation="MNISFNWLKKYVDINLNPEEVANILNTIGLEVEGMELIEQVPGG
                     LAGVVVGEVLECYKHPDADKLRVTKVNIGQGDPLQIVCGAPNVAAGQKVLVATLGTTL
                     LFNSGEKVKIKKSKIRGIESQGMICAEDELGLGASHEGIMVLPEGAVPGTAAKDFFNF
                     KNDTLFEIGLTPNRVDAASHIGIARDLSAYLRLNKCGGEMHIPDVSSFDDLERSDDPA
                     KSIDIEVIASEGAPRYSGLTFDNIKIAPSPEWLQSALKSIGLRSINNVVDITNFILFE
                     TGQPLHAFDREKIEGDRVVVRYARNGERFVTLDGVERNLHPEDVMICNVNRPMCIGGV
                     FGGLDSGITDKTTSIFLESAYFNPVSIRKTSKRHGLKTDASFRYERGADPDIVPYALK
                     RAAILLQEVAGARIVGEIKEVYPKKIERPRIKIDLKRIESFIGKNIGKETILKILDCM
                     DFEIVCQDEASVDVLSPLYRVDVTRECDVVEELLRIYGYNNIELPERVVSSINTTSRP
                     DVEKVRNVASDLLVSNGFYEMMNNSLTKSEYYSKLKTFPQSSLVRILNPLSSDLDCMR
                     QTLLLNGLEVVSHNINHQQTDLKLFEFGNVYSITPNADPTELNSYIEQQKISLFVTGN
                     GALTWKGGTGSSSYFLLKGYLEMLLRRFGIELADLSYLMAPEDIFSEGLLYLTNDGKE
                     ISTIGSISNNLLKQFGIKQKVYAAEISWAPLLNFIKRNKITFRELPRFPEVRRDLALL
                     VDETVSFAELRALAFKTEKKLLKKIALFDVYKGDKIPSGRKQYAISFVLQDPDKTLTD
                     KYVEEVMNRLLTAFTDKFGATLRN"
     gene            8289..9161
                     /locus_tag="P5293_04030"
     CDS             8289..9161
                     /locus_tag="P5293_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HRR49104.1"
                     /translation="MSISIWRRGADYLSLVKFAHTVFALPFAIIGFFLAMKIDCQEFS
                     WKIFILVLCAMVFARNSAMGFNRYVDRFIDAVNPRTAGREIPAKKLSPSSVLIFVIVN
                     VSLFMLTAALINRLTFILSLPALIIILCYSFLKRFTYLCHYGLGFALAIAPVGAYISM
                     TGSFHIVPLILSAIVFLWSSGFDILYSLDDEEFDKNHAIHSIPAKFGRKRAMIISAVG
                     HALVLPLLLLFYYYGMMGTKYIIGAVVFILLLIYQHLIIRPTDISRLNAAFFTSNGVA
                     SLLFAIFTVWDILA"
     gene            complement(9250..10521)
                     /gene="eno"
                     /locus_tag="P5293_04035"
     CDS             complement(9250..10521)
                     /gene="eno"
                     /locus_tag="P5293_04035"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRR49105.1"
                     /translation="MEISNVFAREILDSRGNPTLEVDVELLSGAFGRASVPSGASTGS
                     NEALELRDGDKSRYMGKGVLQAVKNVNDIIAPEILGMSALNQVQIDRTMIELDGTPNK
                     SKLGANAILGVSLAVAKAAADYLQIPLYRYIGGTNAVTLPVPMMNIINGGSHSDAPIA
                     FQEFMIRPAGAQSFREGLRMGAEVFHNLKKIFHDRGLSTAVGDEGGFAPNLNGTEDAL
                     ESIIAAIKKAGYIPGKDVMIALDCAASEFYSNGIYDYTKFEGTKGAKRNAKEQVAYLK
                     SLVAKYPIDSIEDGMSEDDWDGWKLLTEELGSTTQLVGDDIFVTNVEFLRKGIEMGCA
                     NSILIKVNQIGTLTETLNCIELAHRNKYTTVTSHRSGETEDTTIADIAVATNSGQIKT
                     GSLSRTDRIAKYNQLLRIEEELGKLAQYGRK"
     gene            10582..>11492
                     /locus_tag="P5293_04040"
     CDS             10582..>11492
                     /locus_tag="P5293_04040"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HRR49106.1"
                     /translation="MKSRTFILISILALIFSITSNAQVTSGLRIVSGKVDTVFRASHY
                     IVGAAKPGSEIFINGISVKQYKTGSFGMEINLVEGNNPIEVRVRNSNEDIRESFSVYY
                     KLQSPQSMRKSVKDTLPYNGLVITTLPGAYLNYSAGDDRLGGAKINFISEGINMAVID
                     STGDLYKVRLSENRYCFLPKEYAKKVPFGTNPPFTITGSWSVSKNDKCDRVRISLQER
                     VPYTLSFELDPSKIIVDLYSAQCNSNWITQYLDLEAIDYVDFAQIESDVFRVIIKLKS
                     NKCWGYTAEYSDNSLVISVKHSPNPTL"
BASE COUNT         3611 a         1842 c         2430 g         3509 t
ORIGIN      
        1 gtgatcatag aaaaattggg aaagagcttg aactgtttgc cttttcacaa aaagttggtc
       61 agggtcttcc tctttggttg cccaagggtg ctcagttaag agaaagactt gaaaattttt
      121 tgaggattgt tcagaaaaaa ggcggttacc ttcctgtcat cactcctcat attgggcaga
      181 aagaacttta tgtcacttcc gggcactatg caaaatatgg tgcagactca tttcagccta
      241 ttcatacccc acaggaggga gaagagtttc tattaaaacc tatgaattgt cctcatcatt
      301 gtgaaatata taagacaaaa ccacgttcat acaaggattt gcctcttcgt tttgcggaat
      361 ttggcacagt ttatcgttat gagcagagtg gagaacttca tggacttacc agagttagag
      421 gttttacaca ggatgatgct cacatttttt gtcgccagga tcagataaag gaagagttta
      481 acaaggttat agacattgtg ctttatattt ttaagacttt ggattttaaa gattttacag
      541 cacaaatttc acttcgtgac cctgaaaaca aagagaaata tattggtagt gacgaaaatt
      601 gggaaaaggc ggaaagggct attattgaat cggctcacga gaagggactt aatacggtca
      661 ttgtgcttgg tgaagcagcg ttctatggcc caaaactgga ttttatggtc aaagatgcta
      721 ttggcagaaa atggcaactt ggtacaattc aggtagatta taatttgccc gaaagatttg
      781 atcttgagta tatcggctct gatgacaaga aatacaggcc tgtaatgata catagagctc
      841 cgtttggctc tatggagaga tttgtcgcag tgcttattga acatacgggc ggtaaattcc
      901 cattgtggct tgctcctgat caggctgtta ttcttcctgt tagtgagaag tataacgaat
      961 atgcaaaaaa agttgtagat ttgctgacta ataacgatat tcgcgcccta attgacgacc
     1021 gaaacgaaac tattggcaaa aaaatcagag agaatgagtt aaaacgcatt ccttacattc
     1081 tgattgttgg agaaaaagaa gctgaggcag ggactgtttc tgtcagaaga cagggcgaag
     1141 gagataaagg cacaatgact ccagataatt ttgttgaagt ggttaataga gagattaaag
     1201 aacaaatagg atattaattt aaaataggag gatattttat cgaatcgact tttaaacctc
     1261 gccagggtgt atcatcgtca agaacaccgg gctcaaacaa atcgggcaat cccagcaggg
     1321 agcatcaata caggaaagct ccagtacagg atggcccgag agtaaataat gaaataactg
     1381 caccaaaagt gagagtggta ggggagaata tacaaaaccc cggtatttat tcattttcgg
     1441 aggcaattaa gatggcagag gcgttagagt tggatctggt tgaaatatct cctaatgcag
     1501 atcctcctgt ttgtaagatt gtagattatc aaaaatatct ttatcagcag aagaaaaagg
     1561 caaaggagat gaagtccaat gccagcaagg tggttataaa ggagatacgt tttggtccaa
     1621 acactgatga gcatgactat caattcaaac ttaagcatgc tcaggcgttt cttcaagagg
     1681 gatgtaaagt aaaggccttt gttttcttta agggaagatc aattatcttt tcggatcaag
     1741 gggaaaaatt gcttttaaga tttgccctcg accttgagga ttatggaaag gctgaacaga
     1801 tgccaaaact ggaagggaaa aggatgataa tgatgcttgc gcccattaag aaaaagtaat
     1861 tttaactttt aaaataatac atagatgcct aagatgaaga ccaacaccgg tgcaaaaaaa
     1921 cgatttgaac ttaccggcac cggcaaagtg aaaagaaagc atgctttcaa aagtcacatt
     1981 ttaacaaaga aatctaccaa gagaaagaga aaccttactc atactgctat tgtttcgcca
     2041 gcagacgaga aaaaggttaa agatcttttg gtttcataat tatttattaa cctgaatgtt
     2101 cagcagaaca agtctcttgt ggagcagagc gctgacgttc ttaaacaaaa caaaagatgc
     2161 caagatcagt taattcggta gcttcaagag cacgccgtaa aaaaattcta aaacagacac
     2221 gcggtaattt ccttgcacgc aaaaatgtat ggacagttgc taaaaatact tgggaaaagg
     2281 gtttaaccta tgcgtaccgc gaccgtaaaa ccaaaaagag aaattttaga gctctttgga
     2341 ttcaaagaat aaacgctgct gcaagacagc atggaatgaa ctattcacag tttatgggga
     2401 aacttgcaaa gaataatgta gggcttaacc gtaaagtgtt ggcggattta gcaatgaatg
     2461 atcctaaggc ttttgaggca ctcatcaatt caataaagta aacagagttg aaagttcgga
     2521 agataatagg caacagatgg ttcaaatttt ccgtttgggc agttctttat ttgttgtggg
     2581 taatttggct ggaaaattgg tggtttcttt taggattaat aatcatcttc gatatcttta
     2641 ttacccgcaa ggtaaagtgg gccttttgga aaaagaacta taaaaaagga gaaaaacgaa
     2701 atgtatggct tgattggctg gatgcgataa tttttgcatt aattgcttct gcatttataa
     2761 agggtttttt ctttgaggct tacatgatac ccacttcatc tatggagaga acattgatga
     2821 ccggtgacta tctttttgta agcaaggttg cttatggtcc aagggtgccc atgacaccaa
     2881 tttctgttcc acttattcac aatgtgcttc caattaccgg aggagagtct tatgttaaat
     2941 ggattcaaaa tccatatagg agaatggcag ggttcaggag tgtaaagcgt gacgatattg
     3001 ttgtctttaa ttttccaaat ggggatactg tgctaaaggg agatcctaca gctgactact
     3061 atcagtgtat caggctatat ggcagagaat atacttataa gacctacggt cctgtagtag
     3121 taagacctga tgacaaaaag gacaattatg ttaagagatg tgtggcaaca gcaggtgata
     3181 ctcttgagat tataaacggc aaagtttatg tgaacggatt acctcagaat aattatccag
     3241 gtattcagaa tacttttacg gtatacacaa atggtactac catcaattca aagataattg
     3301 atgatatgga tatcaatcca aatgaacttt actttgatgt tagtctgcca ggatacataa
     3361 attttccgct gaatgaagat gaagttgaaa aaatcagagc aatggcaaat gtaatagatg
     3421 ttcgtcagaa cattgacaca tatcctcctg atcttccgga ttcgcccctc actatttttc
     3481 cttttgaggg tgatttcaga tggactcggg acaattttgg gccattatgg gtgcctaaaa
     3541 agggaacaac agttaaactt actatagcca atcttcctct ttacaaacgc attatttcat
     3601 cttatgaaga caactctctt gaagttaagg attctttgat atttattaac ggaagagttg
     3661 ttgacagcta tacttttaag atggattatt actttatgat gggtgataac agacacaatt
     3721 ctgctgattc cagatattgg ggctttgttc cggaatcaca tgtggttgga acgccttcag
     3781 taatttggtt ctctaaagat aagaacaagt cttttccaaa aaatataagg tggaaaagga
     3841 tttttaaaac agtataaata ttttgataat acagaaaaaa tcatgatatt tgcagcccct
     3901 tatgggtggt aaggaatgaa ttaataaata aatgaacaaa gatggcacga gtttgtcaaa
     3961 taaccggtaa gagaagaatg atagggaata atgtttctca ttcaaaacgc cgtacaaaga
     4021 gggaattttc tccaaatttg aagaataagc gtttttggtt ggaagaggag cagagatttg
     4081 ttactcttaa ggtttctact gcaggaatgc gaaatattta taaaaacggg ctggctgcta
     4141 cacttagaaa agctcaaaaa aatggtttga ttgacttata ttaagtatta agtttcggat
     4201 attatggcaa agaaaggaaa tagagtccag gttattcttg agtgtataga acagagagag
     4261 agtggtgtgc ctgggatttc aagatatatt accacaaaaa acaaaaagaa taccactcag
     4321 cgtttggaac gcaagaaata taatccttac ttaaaaaagg tgacaattca ccgagaaatt
     4381 aagtaactaa ggctaaatta ttaaacaatg gcaaaaaagg tagttgctac attcggtagc
     4441 ggaaaaggaa ctgggaaaaa tgttgttaag tgtatccgca tggttcgttc agaacgtaca
     4501 ggagcttata catttgagga ggagattgtc ccgacagagg gagttaaaga tttttttgcg
     4561 aggaaatagc tcctctgttg agaatataag ctaaggggtg tgtcaggtta tcatgacaca
     4621 ccccttagct ttaaacttca ataatattat gttatattaa aattgatatc tttgtaaatt
     4681 atttttcgaa aatggcatta tttggattgt tcggatcaaa atcaaaggag gagaaagagg
     4741 ctctcgacaa agggcttgag aagacaaaaa aaggtatttt tgagaagctt tcaagagcag
     4801 tcgtaggtaa atcaaaagtt gacgatgaca cactggatga tattgaagag gctctgattg
     4861 cgtctgacat tggcgttgaa actacattaa agattattga caggcttgag gcaagagttc
     4921 atagggataa atttatggac agcaatgagc ttaatcagat tctaaaggag gaaatagagg
     4981 ctttgctcaa tgttgatgac aatcatgagg gtgatatgcc ttttgatttt tcaaaaaaac
     5041 catatgtcat tcttgttgtt ggagtgaatg gagtaggtaa aactacaaca attggaaaac
     5101 ttgcggcacg tcttaagaat tcaggatata aagttgttct tggtgctgca gatactttta
     5161 gagctgcggc tgtcgaacag ttgaccatat ggagtgagcg tgcagnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnngatac ttttagagct gcggctgtcg aacagttgac
     5341 catatggagt gagcgtgcag gagtgccaat tgtaaagcag gcaatgggag cagaccctgc
     5401 ttcagttgct tatgatacag tttcttctgc gattgctcag ggtgcagatg tggtgcttat
     5461 agatactgca ggtcgtcttc acaacaaggt taatcttatg aacgaactta ctaaaataag
     5521 aaatgttatt aaaaaaatta ttcctgatgc acctcacgaa gttttacttg tattagatgg
     5581 atctacaggc caaaatgcct ttcagcaagc taaggaattt acaaaagcca cagatgtgac
     5641 ggcacttgca attacaaaac ttgacggcac agctaagggt ggagtagtta ttgggatttc
     5701 agatcaattt aagattcctg ttaagtttat tggtgttggg gagaaaattg aagatttgca
     5761 gattttcaat aaaaaagaat tcgtagaatc tctgttttaa agttattaat aaattgttat
     5821 gaatatatcg tttaattggc tcaaaaaata cgttgatatt aatttaaatc ctgaagaagt
     5881 tgctaatatc ttaaatacaa ttggtctcga agttgaaggg atggaattga ttgagcaagt
     5941 tcctggaggc cttgctgggg ttgtcgtcgg tgaagtattg gagtgttata agcaccctga
     6001 tgcagacaag ctcagggtta ctaaagtaaa tattggtcag ggtgatccac tgcaaattgt
     6061 atgtggcgct cctaatgttg cagcggggca aaaagtcttg gttgctacac ttggaacgac
     6121 tcttttattt aacagcgggg aaaaggttaa gattaaaaaa tccaagatta gaggcattga
     6181 gagtcaggga atgatatgtg ctgaagatga attgggactc ggtgcttctc atgaaggaat
     6241 aatggtactt cctgagggtg ctgttccagg tactgcagca aaagactttt ttaattttaa
     6301 gaacgatact ctttttgaaa ttggattaac accaaataga gttgatgcag cttcacatat
     6361 tggaattgca agagacctgt cggcgtattt aaggcttaat aaatgtggag gagagatgca
     6421 cataccagat gtctcttctt ttgatgattt ggaaaggtct gatgatccag caaaaagtat
     6481 agatattgaa gttattgctt ctgaaggagc tccaaggtat tcaggattga cttttgataa
     6541 tataaaaatt gctccttctc ccgagtggtt acaaagtgca ctgaaatcga ttggcctgcg
     6601 atctattaat aatgtcgtgg atattaccaa ttttatttta tttgaaacag gacagccact
     6661 gcatgctttt gacagagaaa aaatagaagg agacagagtt gttgtaaggt atgcaagaaa
     6721 tggagaacgc tttgttactc ttgatggtgt tgaaagaaat cttcatcctg aggatgtcat
     6781 gatctgtaat gtcaacaggc cgatgtgcat cggaggtgta tttggaggtt tggattcagg
     6841 tattacagac aaaactacat ctattttttt ggagagtgca tatttcaatc ctgtttcaat
     6901 cagaaagaca tccaaaagac atggtttaaa gacagatgcc tctttcaggt acgaaagagg
     6961 tgcagatccg gatattgttc cctatgcact taaaagagca gctatcctgt tacaggaagt
     7021 cgccggcgca cgaattgttg gtgaaattaa agaggtctat ccaaagaaga ttgaaaggcc
     7081 gagaataaag atagatttaa aacgaattga gagtttcata ggcaagaata ttggcaaaga
     7141 gacaattctc aagattcttg attgtatgga ttttgagatt gtatgtcagg atgaggcttc
     7201 tgttgatgtt ctttctccac tttacagggt agatgttaca agggagtgcg atgttgttga
     7261 ggagctgctt cgtatatatg gatataataa tatagaatta ccagaaagag tcgtttcatc
     7321 tattaataca acctcaagac cagacgttga aaaagtaaga aatgttgctt cagatttact
     7381 tgtttctaat ggtttttatg agatgatgaa taattcactc accaaatcgg aatattattc
     7441 taaactaaag acatttcctc aatcttctct tgttagaata ttaaatcctc tgagttctga
     7501 cctcgactgc atgcgccaga ctcttttgct aaacgggttg gaagttgttt cacataatat
     7561 aaatcatcag caaaccgatc tgaaactttt tgaatttggc aatgtctatt caataactcc
     7621 aaatgcagat ccaactgaat tgaattcata tattgaacaa caaaaaattt ctctctttgt
     7681 tacaggaaat ggagccttaa catggaaagg tgggactggt tcctcttctt attttcttct
     7741 gaaaggttat cttgagatgc ttttaagaag atttgggatt gaattggctg atttgtcata
     7801 tttaatggct cctgaggata ttttttctga aggactttta tatttgacaa atgatgggaa
     7861 agagatatca actattggct caataagcaa taatcttctt aagcagtttg gaattaagca
     7921 aaaagtttat gcagctgaaa tatcgtgggc acctctttta aactttatta agagaaacaa
     7981 aataacattt agagaacttc caaggttccc tgaagtcagg agagatttag ctttactcgt
     8041 ggatgagaca gtctcttttg cagaattaag agctcttgct tttaagacag aaaaaaaatt
     8101 gcttaaaaag attgcgctat ttgatgttta caaaggagac aaaatacctt caggcaggaa
     8161 acagtatgca atcagttttg tgttgcagga tcctgataag actctgacag ataaatatgt
     8221 tgaggaggtt atgaacaggc tgcttactgc gtttacagat aaatttggtg ccacattaag
     8281 gaattaagat gagtatttcg atatggagga gaggggcaga ttatctgagt cttgttaaat
     8341 ttgctcacac tgtatttgca ttaccatttg ccataattgg ctttttcttg gcaatgaaga
     8401 ttgactgtca ggaattttcg tggaaaatat ttattcttgt actttgtgcc atggtgtttg
     8461 caagaaacag tgccatgggt tttaacagat atgtagacag gtttattgat gcagttaatc
     8521 ctagaacggc cggaagggaa atccctgcca aaaagttgtc gccatcatca gtattgatat
     8581 ttgtgattgt aaatgtctcg ctttttatgc tgactgctgc attaattaac aggttgactt
     8641 ttattctttc cttacctgct ctcataataa ttctttgtta cagttttctg aagcgcttta
     8701 catatttgtg tcactatgga ttaggctttg ctcttgccat tgctccagtt ggtgcatata
     8761 tcagtatgac aggttcgttt catatagttc ctcttatttt gtctgcaatc gtatttttat
     8821 ggtcgagtgg ttttgatata ttatattcac ttgatgatga ggaatttgat aagaatcatg
     8881 ctattcactc gatacctgca aagtttggac gaaaaagagc catgattatt tcagcagtcg
     8941 gacatgcttt ggttttaccg cttttattgc tgttttatta ttatggaatg atgggtacaa
     9001 aatacataat aggagcagta gttttcattt tgcttcttat ttatcagcat ttaatcatta
     9061 gacctactga tatttcccgt ctgaatgctg ctttttttac ctctaacgga gttgcatctc
     9121 ttttgtttgc aatttttact gtttgggata tattggcata aaagttccat aacggaacca
     9181 ttctcataaa aaaaggcgcc caaaataact ttttgaacgc cctttgaaaa atctatggga
     9241 ttgttaatat tattttctac catattgtgc aagttttcca agctcttctt caattctaag
     9301 aagttggtta tactttgcaa ttctgtcggt tctgctgaga gaaccagttt taatctgacc
     9361 ggaatttgtc gcaactgcaa tatcagcgat tgttgtatct tctgtttctc cggagcggtg
     9421 agatgtgact gtagtatatt tgttgcgatg tgcaagctct atgcaattga gcgtttccgt
     9481 aagtgtgcct atttggttta ctttgataag gattgaattg gcgcaaccca tctcaatgcc
     9541 cttgcgcagg aattccacgt ttgtaacgaa tatatcatca ccaacaagct gcgttgttga
     9601 gccaagttct tcagtaagaa gtttccatcc atcccagtca tcttctgaca tgccgtcttc
     9661 tattgagtca attggatatt ttgcaacaag gctcttaaga taagcaacct gttcttttgc
     9721 atttctcttt gcccctttag taccttcaaa ttttgtgtaa tcgtaaattc cattgctgta
     9781 aaattcactg gcagcacagt caagagcaat catgacatct tttccgggaa tgtatccggc
     9841 ctttttgatg gcagcaataa tagattcgag tgcatcctct gtgccattta gattaggagc
     9901 aaacccacct tcgtctccaa cagctgtgct gagacccctg tcatggaaaa tcttctttag
     9961 attgtgaaaa acttctgcac ccattctaag gccttctctg aaagattgag caccggcagg
    10021 acgaatcata aattcctgaa atgctattgg ggcatcagag tgtgatccac cattgatgat
    10081 attcatcata ggtactggta gagttactgc atttgtgccg ccaatgtatc tgtaaagtgg
    10141 aatttgaaga taatctgcag cagccttggc aacagcaagt gatacaccaa gaatggcatt
    10201 agctccaagt ttcgatttgt taggagtacc atccaactct atcattgtcc tatcaatttg
    10261 tacttggttg agagcgctca ttcccaatat ttcaggtgca ataatgtcgt taacattttt
    10321 aacggcctgt aatacacctt tacccatata gcgagattta tctccgtctc tcaattcaag
    10381 tgcttcatta ctccctgtag atgctccgga aggtacagat gctcttccaa aagctcctga
    10441 caatagttca acatcaactt ccaaagtagg atttccgcgt gaatcaagaa tctcgcgagc
    10501 gaaaacatta ctaatttcca taattaatta tatagttatt tggttaaatt tgtataatat
    10561 tttaataaca aatatcaagc catgaaatca agaactttca ttctgatatc aattttagca
    10621 ttaattttct caatcacatc caatgcacaa gtaaccagtg gtttgagaat tgtcagtggt
    10681 aaagttgata ctgtattcag agcttcacat tacattgtag gagctgcaaa accaggatct
    10741 gaaattttta taaacggcat aagcgtaaag cagtataaaa caggctcttt tggtatggag
    10801 attaacttgg tagaggggaa taatcctata gaagtgagag ttagaaattc aaatgaggat
    10861 atcagagaaa gtttctctgt atattacaaa ttgcaatcac ctcagtcaat gagaaagtct
    10921 gtaaaggata ctctgcctta taatggatta gtaattacaa ctcttcccgg agcctatttg
    10981 aattatagtg caggtgatga cagacttgga ggagcaaaga taaatttcat ttcagaaggt
    11041 attaatatgg ctgttatcga ttcaacagga gacctttata aagtcagatt gtctgaaaac
    11101 agatattgtt ttttacctaa ggagtatgct aagaaggttc catttggaac taatcctccg
    11161 tttactatta ccggttcatg gagtgtttca aagaacgata aatgtgaccg agtaagaatt
    11221 tcacttcaag agagagtacc ttacacactt agttttgagc ttgacccttc taagataata
    11281 gtagatttgt atagtgctca atgcaattct aactggataa ctcaatatct tgatttggaa
    11341 gccatagatt atgtggattt tgcacagatt gagtctgatg ttttcagagt aattattaag
    11401 cttaaatcca ataaatgttg gggatatact gcggagtatt ctgacaactc tttagtaatt
    11461 agtgttaaac attctccaaa tcctactctt aa
//
LOCUS       DAOTQL010000045        10954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5425_length_10954_cov_4.502340, whole genome shotgun sequence.
ACCESSION   DAOTQL010000045 DAOTQL010000000
VERSION     DAOTQL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10954
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5425_length_10954_cov_4.502340"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="ablA"
                     /locus_tag="P5293_04045"
     CDS             <1..1135
                     /gene="ablA"
                     /locus_tag="P5293_04045"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRR49107.1"
                     /translation="IKLTAEEEEGVKKSLETLRMAITPYYLSLIDPDNPDCPIRKQAI
                     PTGAETYRSAADLVDPLHEDEDSPVPGLTHRYPDRVLLLITDMCSMYCRHCTRRRFAG
                     QKDDESPANNIDRAIDYIARTPQVRDVLLSGGDALMVSDAKLESIIKRLRAIPHVEII
                     RIGTRTPVVCPQRITDKLVDMLKQYHPIWLNTHFNHPNEVTPESIAACEKMANAGIPL
                     GNQSVLLRGVNDCVNIMKKLVHLLVKMRVRPYYIYQCDLSMGLEHFRTPVSKGIEIIE
                     GLRGHTSGYAVPTFVVDAPGGGGKIPVMPNYLISQSPGKVILRNYEGVITTYTEPDNY
                     VNQCHCPDCLKNEAVEGVSALLKGQKLSLEPEHLIRKERSHKH"
     gene            1143..2165
                     /locus_tag="P5293_04050"
     CDS             1143..2165
                     /locus_tag="P5293_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49108.1"
                     /translation="MSLVQVVLKYRSLSIVGMEKNTGKTECFNYLVRKLPDECLNIAV
                     TSIGLDGERVDSVTGTKKPEIMLRRGMYFATSEKLYKKKEILSEVVNVSESSGSLGRT
                     ITAKALASGKVMLAGPPATVQLRKWMEEMTIKFKTRMCIIDGAISRMSLASPAVSEAM
                     ILTTGAALSMNIDSLVQKTSRMVDLIKLPVTQICSYEKLKIISNGIWGIICGEELIDL
                     NISSSLEIEKVDTDLLSKLEAIFISGALTDIFLKILTDRIDVSNLEIIVRDFSRIFAN
                     HKTLFNFERRGGKIRVLQKSNLIAICVNPVSPNGATLNSERLCSELSKVIKLPVYDII
                     REDYEA"
     gene            2155..3519
                     /locus_tag="P5293_04055"
     CDS             2155..3519
                     /locus_tag="P5293_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49109.1"
                     /translation="MKLRTFLDSQSSFRFFVERLNISSSHARNVLLDTQFPDTLKEIK
                     SAYQSLKTFYDQFIKEGKFKYTILHVQEKLSEVRDIRGSLSRLEKGNLLDDIELFEIK
                     SISLISEDIKHIFNETGINTEYLHFSDLSPVVEILDPEGNRINSFYVYDAYSEELALI
                     RKELKSKEVFDAELYYKASLIEEKVRERLCADLIKYVELIGDSLKTLANLDIIIAKAL
                     QVKELNLCFPDYSKGTINYKALVNPEIEEYLSKEGKVFQPVDISIETQKPLLITGANM
                     GGKSVSLKSLFTAQLLFQFSFGIPAKNASISYVDNLFLITGDNQSISKGLSSFAAEMI
                     AIDSVIKEVVSGKKILALIDEPAGTTNPTEGTALVSSLLKRLSGQCSYVVITTHYNIE
                     NSECRRLRVRGYIDGKMDYSLIPVNEIEAPREAVNIARSLNVDDIWINEAELIVNQKN
                     KYAK"
     gene            3509..5065
                     /locus_tag="P5293_04060"
     CDS             3509..5065
                     /locus_tag="P5293_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HRR49110.1"
                     /translation="MQSKLGLDFKRVEYARQIAKSIADDVQNFVSNYTTIAVERTLCR
                     LLGIDGVDSNQVPLPNVVTDELKSKGVLSHGAVFYISNAMVNTSLNPQEIAEKIANET
                     LDITKLNVVPYEERMKAIEPLIKASIEKIKSNRAKREQYLQTLGEGSRPYLYVIVATG
                     NIYEDVVQAEAAARQGADIIAVIRTTGQSLLDYVPYGATTEGFGGTYATQENFRIMRK
                     ALDKVGEEIGRYIRLCNYCSGLCMPEIAVMGAFEGLDVMLNDALYGILFRDINMQRTL
                     IDQYFSRIINGFAGVIINTGEDNYLTTADAYEEAHTVLASDLINEQLASLAGIPEEQM
                     GLGHAFEMDPELTNGFLYELAQAQMTREIFPNAPLKYMPPTKYMTGNIFKGHVQDALF
                     NIIGIWTHQGIQLLGMLTEAIHTPFMSDRYLSIENAKYVFNNMRNIGDEIVFKEGGIV
                     RNRASQVLEGAISLLERIDNEGLFSALEKGIFANVKRPRNGGKGLEGVVEKGNNYINP
                     FIGIMKGGKR"
     gene            5065..5871
                     /locus_tag="P5293_04065"
     CDS             5065..5871
                     /locus_tag="P5293_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HRR49111.1"
                     /translation="MSSGLYSMQTQDFDKTLDLSKVKPYGDTINDGKVQLSFTLPVPS
                     GAEAVEAAKQLLKQMGFENPQVVFYRELTPGFTFFNCYGSTVHTVNYSDIHVPKVESQ
                     VMSMEETDRFIRENIGRPLVVIGASTGTDAHTVGIDAIMNMKGYAGHYGLERYEMIEA
                     HNLGSQVPNEEFVAKAIELKADALLVSQTVTQKDVHIKNMVELVELLEAEGIRDKVIL
                     ICGGPRITHELAQELGYDAGFGMNSYADDVASFIAQEFVRRNKKVNNYQK"
     gene            5877..6539
                     /locus_tag="P5293_04070"
     CDS             5877..6539
                     /locus_tag="P5293_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRR49112.1"
                     /translation="MDKNSMREVIAKRAALELKDGYVVNLGIGLPTLIPNYLPAGVSV
                     ILQSENGLLGMGPEPEPGKEDDNYTNAGGGYITYTKGASCFDSSLSFAIIRGGHVDVT
                     ILGALEVDEQGNLANWMIPGKKSPGMGGAMDLITGAKRVIVAMEHTAKGAPKILERCT
                     LPLTAAGKVNMIITEMGVMHILPEGKIMLTEINPEFSVEDVQQATGAKLIISDNLIKM
                     QV"
     gene            6542..7747
                     /locus_tag="P5293_04075"
     CDS             6542..7747
                     /locus_tag="P5293_04075"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRR49113.1"
                     /translation="MKKVFIVAAKRTAIGKFMGSLSPFNPGELGAIVIRELLNETKID
                     PAVIDTVIVGNVLSAGQMQGVARQVSIKGGVPYEVPAYSINMVCGSGMKAVMNGYAGI
                     ISGAADVVIAGGTESMTNAGFILQSSLRSGVKMGDLKVIDHMVYDGLTDAFNNYHMGI
                     TAENIAERLGISREEQDVFAYASQQKAIKAVDSGAFVNEIVPVKIESKNGTVIFDKDE
                     FPNRASTPEKLAALKPAFKKDGTVTAGNSSGINDGASFVMIMSEDAVAKYGMTPLCEL
                     IAVGQGGVDPREMGLGPIPAIRKALKNASMKFEDIERIELNEAFAAQSLGVIKGLIEE
                     YKVTREWIDERTNLNGGAIALGHPIGASGNRIIVTMIHGLIATGKNIGLASLCIGGGM
                     GTAVIVKRV"
     gene            complement(7841..9115)
                     /locus_tag="P5293_04080"
     CDS             complement(7841..9115)
                     /locus_tag="P5293_04080"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR03844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HRR49114.1"
                     /translation="MNNYKLMNISTGRVFDDAGWVLSDPESPTPSLLRSVYPNKKFEL
                     NNNDYGFYRYASWLPICRTLENSASTITYRSNGLANRLGLDNLFISFSGYWPEIGANM
                     TTCSFKETEAYSVCARFHPDNNKIMVVASAGNTARAFAKVCSDNKIPLVVVIPEDNLD
                     ALWFEKPLNKCVKIVCTPSQTDYFDAIDLSNKICKSPMFFEEGGAKNVARRDGMATTL
                     LSAVNTIGRIPDYYFQAIGSGTGTIAVWEANLRLIEDGRFGNHKMILIPSQNFPFTPM
                     FDAWKVRSRELLPFDDDEARKKALQIKAKVLSNRRPPYSITGGLFDALKDTNGDIEIA
                     TNDESEAACELFKETEGIDIYHASGIALAGMIKSIKSGKVSKDKVIMLNITGGGENKF
                     KSTHDYVMAKPDLVIDNNGDTGTICKSVENLF"
     gene            complement(9143..>10954)
                     /locus_tag="P5293_04085"
     CDS             complement(9143..>10954)
                     /locus_tag="P5293_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRR49115.1"
                     /translation="SLYGGTAMKERFLFGYDPFVLPFTLGMVFIILYLLVGTARIIIS
                     LTHEEQISLLKSLKPKQLFKTIKDVITDCLLHIKIFKRNPLLGYMHFSIAFGWFMLIV
                     VGHIEVVLYTPQRNGILYYPVFFRYFVMETQQTLRGAFFFFLMDLFLLMVLSGIALAI
                     FKRFRSRALGMRRTTKLKIGDTVALYSLWAIFPLRLLAESFTADISGGSFLTKSINHL
                     VHGFVSNPDNALPIWWSYSIALGVFLFALPFSRYMHIPTEILLIFLRNAGIKATSFRN
                     GYAESEIYSCSRCGICIDACPMGVNLGDIKFSTVYTIRLLRKRENALEGAEKCLMCNK
                     CVEVCPVGINSTQLKLLYRQSFNDTGDHDFSYLSLQNVKHNKEKILYYAGCMTHLAPA
                     IYLSLFKILEKAGIEYNFMDADGSICCGRPLMLSGDFKGASQLIDKNKDIILNSGCTT
                     LLLSCPICYKVFREEYNLQGVEIIHHSVYINDLLNSGKIKVDRVHESFVFHDPCELGR
                     GSNIYDEPRELLSSIGILKDSPEEKGESICCGGSLGSLTMQYERRKRITEHSLSMLYT
                     NNPDTLVTACPLCLKTFSPVNSKKTVDIAQVVSEHLI"
BASE COUNT         3573 a         1815 c         2225 g         3341 t
ORIGIN      
        1 tattaagttg acagcagaag aggaggaagg tgttaagaag tcacttgaga ctctcagaat
       61 ggctataact ccttattatc tttcattgat tgatccagac aaccctgatt gtcctatcag
      121 aaaacaggca atccctacag gagcagagac atatcgctct gcagcagacc ttgttgatcc
      181 tcttcatgaa gacgaggatt ctccggtgcc cgggttgact cacagatatc ctgacagagt
      241 tttgcttctg ataaccgata tgtgttctat gtactgtaga cactgtactc gtagaagatt
      301 tgccggccaa aaggatgatg agtctcctgc aaataatatt gacagggcaa tagattacat
      361 agcaagaaca ccgcaagtca gggatgtcct tttgtcggga ggtgatgctc taatggtttc
      421 ggatgctaag cttgaatcta ttatcaaaag attacgtgct attccacatg ttgaaattat
      481 aagaattggc accagaacgc cggttgtttg tcctcaaaga attactgaca agcttgttga
      541 catgcttaaa cagtatcacc caatatggct aaatacacat tttaatcatc caaatgaggt
      601 tactccagaa tcaattgctg catgtgaaaa aatggcaaat gcaggtatcc ctcttggaaa
      661 tcaatctgtg ttgcttagag gcgtaaatga ttgtgttaat ataatgaaaa aacttgttca
      721 tcttcttgtt aagatgagag tccgaccata ttacatatat caatgtgatc tctcaatggg
      781 actcgaacac ttcagaactc ctgtttcaaa aggaattgag attattgaag ggttgagagg
      841 tcacacttca ggatatgcgg ttccaacttt tgttgtagat gcacccggag gaggtggaaa
      901 aataccagtt atgccaaact acttgatttc ccaatctccg ggcaaggtta ttttgagaaa
      961 ttatgagggt gtaataacaa cttatacaga acctgataat tatgtaaatc aatgccactg
     1021 tccggattgc ttgaaaaatg aggctgttga aggagtttct gccttattga aaggacaaaa
     1081 actatctctt gaacccgaac atctcattag aaaggaaaga tcacataaac attaataatc
     1141 caatgtcact tgttcaggtt gttcttaaat acagaagtct ctcaatagtg ggtatggaaa
     1201 aaaataccgg taaaaccgag tgttttaatt acctcgtccg caaattgcct gatgaatgtt
     1261 taaatattgc tgtaacatca ataggtcttg atggtgaaag agttgattct gttacaggaa
     1321 caaaaaaacc tgagattatg ctcagaagag gaatgtactt tgcaacaagt gagaaattat
     1381 ataaaaaaaa agaaatcctg tctgaagtag taaatgttag tgaatcatca ggatccttgg
     1441 gcaggactat tacagctaaa gcattggcat cgggcaaggt tatgcttgca gggcctcctg
     1501 ctactgtgca gttgagaaag tggatggaag aaatgacgat aaagtttaaa accagaatgt
     1561 gtatcattga cggcgcaatt tcgagaatga gtttggcttc gcctgctgtt tcggaagcaa
     1621 tgattttgac aaccggagct gctttatcaa tgaatattga tagccttgtt cagaaaacaa
     1681 gtcgcatggt tgatctaata aaacttcctg ttactcagat ctgctcatat gaaaagctga
     1741 aaataatcag taatggtatt tggggaatta tttgtggaga agaattgatt gatctcaata
     1801 tctcatcttc acttgaaatt gaaaaagttg atacagattt actgagcaaa ttggaagcaa
     1861 tatttatttc aggtgcttta actgacattt tcttaaagat tttgacagat agaattgacg
     1921 taagcaactt agaaattatt gttagagatt tttcaagaat atttgctaat cacaaaacgc
     1981 tttttaattt tgaacgtaga ggcggaaaaa taagagttct tcaaaagagc aaccttatag
     2041 ctatatgtgt caaccctgtt tcaccaaacg gagctacact taactcagag agattgtgtt
     2101 cagagctctc aaaagttata aaacttcctg tttacgatat tattagagaa gattatgaag
     2161 cttagaactt ttttggactc acagtcgagt tttagatttt ttgtagagag gctgaatatc
     2221 agttcctcac atgcacgtaa tgttttgttg gatactcaat ttccggatac tcttaaagag
     2281 attaaatctg cttatcagtc tctcaaaact ttttatgacc agtttatcaa agagggaaaa
     2341 tttaaatata caattctcca tgtccaggaa aaactttctg aagttagaga tattagagga
     2401 tctctatcaa ggcttgaaaa agggaatttg ttggatgata tagaactttt tgaaattaaa
     2461 tcgatttcac ttatttcaga agacataaag catatattta atgaaacagg aataaatact
     2521 gaatatttac atttttctga tttgtcacct gttgtagaaa tacttgatcc agaaggtaac
     2581 aggattaatt ctttttatgt ttatgatgcg tattctgagg agcttgcgtt gattcgaaaa
     2641 gaattaaaat caaaggaagt atttgatgca gagctgtatt ataaagcatc cttaatagaa
     2701 gaaaaagtta gagagagatt gtgtgctgat ctgattaaat atgttgaatt gataggagat
     2761 tcacttaaaa ctcttgcaaa tttggatata atcatagcca aagctttgca agtaaaggag
     2821 ttaaatcttt gttttcctga ttattccaaa ggaactatca attataaagc tcttgttaat
     2881 ccagaaattg aggaatattt atccaaagaa ggaaaagtat ttcaacctgt tgatatttca
     2941 atcgagactc aaaaaccact attgattaca ggagccaaca tgggtgggaa aagtgtatct
     3001 ctcaaatctc ttttcacagc tcaactgtta tttcaatttt cttttggaat acctgccaaa
     3061 aatgcttcta tttcatacgt agacaatctt ttcctgataa cgggagataa tcaaagcatc
     3121 tctaaaggat tatcctcttt tgctgcagag atgattgcca ttgattctgt tatcaaggaa
     3181 gtagtctcag gaaaaaaaat acttgcattg atagatgaac ctgcaggaac tacaaatcca
     3241 actgaaggta cggcgttggt ttcttcattg ttaaaaagat tgtcaggtca atgctcatat
     3301 gttgtgataa caactcatta taatattgaa aatagtgaat gcaggcggtt acgtgttagg
     3361 ggctatatag atggaaaaat ggactattct ctgattcctg tcaatgaaat agaggccccc
     3421 agagaggctg ttaatatagc tagatctctt aatgtcgatg atatatggat aaatgaagct
     3481 gaattaattg ttaatcaaaa aaataaatat gcaaagtaaa ttaggattag attttaaaag
     3541 agttgagtat gctcggcaaa ttgccaaatc catagcagat gatgtgcaga attttgtatc
     3601 aaattacaca actattgctg tggaaagaac actatgccgg ctattgggta ttgatggtgt
     3661 tgattcaaat caggtgcctt tgccaaatgt tgtaactgat gaactgaaat caaagggtgt
     3721 tttatctcat ggtgcagtct tctatatctc aaacgcaatg gtaaacactt ccttaaatcc
     3781 tcaggaaatc gcagaaaaaa tagctaatga aactcttgac ataacaaaat tgaatgttgt
     3841 accctacgaa gagagaatga aagctattga gccattaata aaagcaagta ttgaaaaaat
     3901 caaatcaaac agagcgaaaa gagagcaata tcttcaaact ctgggagaag gcagcagacc
     3961 ttatctttat gtaatagttg caacaggaaa catatatgag gatgttgttc aggcagaagc
     4021 tgctgcccgc cagggggcgg acattattgc cgtaattaga accactggac agtcattatt
     4081 ggactatgtt ccatatggag ctacgactga aggttttgga ggaacatatg ctactcagga
     4141 aaatttcagg ataatgcgta aagctttgga caaagtggga gaggagatag gaaggtatat
     4201 tagattgtgt aattactgtt caggcctctg tatgcctgaa attgccgtaa tgggtgcttt
     4261 tgaaggactt gatgtaatgt taaatgatgc tctttatgga attctattta gagatattaa
     4321 tatgcagcgc actttgattg accaatattt ttcaagaatt ataaacggat ttgcaggagt
     4381 gataattaac actggtgaag ataattatct tactacagct gatgcatatg aagaggcaca
     4441 tacagtcctt gcttcggatt taataaatga acagttggca tcattggccg gaatacctga
     4501 ggagcaaatg ggtttgggac acgcttttga aatggatcct gaactcacaa atggttttct
     4561 ttatgagctt gctcaggcac aaatgacaag ggaaatattt ccaaatgctc ctttgaaata
     4621 tatgccacct actaaatata tgacggggaa tatttttaag ggacacgtcc aagatgccct
     4681 ttttaatatt attggaatat ggacgcatca gggcattcaa cttcttggca tgttgacaga
     4741 agctattcat acaccgttta tgtcggatag atatctttcg atagaaaatg ccaaatatgt
     4801 ctttaacaat atgagaaata ttggtgatga aattgttttc aaggagggcg gaattgtgag
     4861 aaacagagct agccaagtgc ttgaaggagc aatctctctt ctcgaaagga ttgataatga
     4921 aggattattt tctgctttgg aaaaaggtat ctttgccaat gttaaaaggc cgagaaatgg
     4981 gggcaaaggt cttgaaggcg tagttgaaaa agggaataat tatattaacc cgtttattgg
     5041 aataatgaaa ggaggaaaga gataatgagt agtggactat attcaatgca aacacaggat
     5101 tttgacaaga cgcttgactt gtcaaaggta aaaccttatg gtgatacaat taatgatggc
     5161 aaagttcaac tcagcttcac attgcccgta ccatcaggag ctgaggcggt ggaagccgct
     5221 aaacaattgc taaagcagat gggatttgaa aatcctcaag ttgtttttta cagggaactt
     5281 acaccaggat ttactttttt caattgttac ggttccactg ttcatactgt taattactct
     5341 gatattcatg tacctaaggt tgaatctcaa gttatgagca tggaggagac agacaggttt
     5401 attagggaga atatcggccg accattggta gttataggtg ccagtactgg tactgatgct
     5461 catactgttg gaattgacgc tataatgaat atgaaaggtt atgcaggtca ttatggactt
     5521 gaaaggtatg aaatgataga ggcgcataat ctaggttctc aagtaccaaa tgaggaattt
     5581 gtagccaagg ctatagaact taaggcagat gctttacttg tatctcagac agtaactcag
     5641 aaggatgtcc acatcaagaa tatggtagaa cttgttgagt tgcttgaagc agaaggtatc
     5701 agagataagg taattcttat ttgcggaggg ccgcgcataa ctcatgagct ggctcaggaa
     5761 cttggctatg acgctggttt cggaatgaat tcctatgccg atgatgttgc atcatttata
     5821 gctcaagaat ttgttagaag aaataaaaaa gttaataatt atcaaaaata aagtctatgg
     5881 ataaaaatag tatgagagag gtaattgcca aaagagcagc tctcgaactg aaagatggat
     5941 atgttgttaa tctgggcatt ggtcttccta ccttaattcc aaactattta cctgcaggtg
     6001 taagtgttat tttacaatct gaaaacggtt tgcttggtat gggaccagaa cctgagcctg
     6061 gtaaagagga tgataactat actaatgccg gcggcgggta tattacatat accaaaggag
     6121 catcatgttt tgactcatcg ctctcttttg caattataag aggtggacat gtagatgtaa
     6181 ccattcttgg tgctctggaa gttgatgagc agggaaatct tgctaactgg atgattccag
     6241 gcaaaaaaag tccaggtatg ggtggtgcaa tggatctaat tactggagca aaaagagtga
     6301 tagttgccat ggagcataca gcaaaaggtg caccaaaaat tcttgaaagg tgtactttgc
     6361 ccttaactgc agccgggaag gtaaatatga ttattactga gatgggagtt atgcatattt
     6421 tacctgaagg caaaataatg ttgacagaga taaatcctga attctctgtt gaagatgttc
     6481 agcaggcaac aggagcaaaa ttgattattt cagataacct aataaaaatg caagtttaat
     6541 tatgaaaaag gtatttattg ttgctgcgaa aaggacagct ataggcaagt tcatgggttc
     6601 actttctcct tttaatcccg gagaattggg tgctattgtt ataagggagt tgcttaacga
     6661 aactaaaatt gatcctgcag ttattgacac tgtcattgtt ggcaatgttc tttctgccgg
     6721 tcaaatgcaa ggtgttgcaa gacaggtttc cattaaggga ggtgttcctt atgaggttcc
     6781 cgcttattct attaacatgg tatgcggttc tgggatgaaa gcagtcatga acggatatgc
     6841 tggtattata tcgggtgctg cagatgttgt tattgcaggt ggtacagaat caatgacgaa
     6901 tgcaggcttt attcttcaaa gctcattaag gagtggtgta aaaatgggag acctcaaagt
     6961 tattgaccat atggtgtatg atggtttaac tgatgcattc aataactatc atatgggtat
     7021 aacagctgaa aatattgctg aaagattggg aatttcacga gaagaacaag atgtatttgc
     7081 ttatgcatca cagcagaaag caattaaagc tgtggattcg ggagcttttg tcaacgaaat
     7141 tgttcctgta aaaattgagt ctaagaatgg aacagttatt ttcgataagg atgaattccc
     7201 aaatagagca agtactcctg aaaaactggc agctcttaag ccagccttta aaaaggacgg
     7261 tactgttact gccggaaact cttcgggcat taatgatggt gcttcttttg tcatgattat
     7321 gagcgaagac gccgttgcaa aatatggaat gacaccacta tgcgaactta ttgcagtagg
     7381 gcagggagga gttgacccta gagaaatggg acttgggcca attccagcga taaggaaagc
     7441 tcttaaaaat gcatcaatga aatttgagga tatagaacgt attgagttga atgaagcttt
     7501 tgccgctcag tcacttggcg ttataaaagg attgattgag gagtataaag tcaccaggga
     7561 gtggattgat gaaagaacaa atcttaatgg aggagcaata gctcttggtc atcctatagg
     7621 agcttcaggg aatagaataa tagttacgat gattcacgga ttgattgcca ctggcaaaaa
     7681 tatcggtctc gcatcactct gtattggagg tggtatgggt actgctgtaa ttgttaaaag
     7741 agtataatat catttctacc aaagagactg cattcagata tattatttaa tacctattgc
     7801 agtctctttg aaaaagtcaa ctattcaata ttttggtaat ctagaaaaga ttttccacag
     7861 atttacatat agtaccagta tcaccattat tatcaataac caagtcgggt tttgccataa
     7921 cataatcatg agttgatttg aatttgtttt cacctccacc tgttatattt agcattatta
     7981 ctttgtcttt tgaaacttta cccgatttga tgctttttat catgcctgcc aaggcaattc
     8041 ctgaagcatg ataaatgtct ataccctctg tctccttgaa taactcacag gcagcttcag
     8101 attcgtcgtt agtggcaatt tctatatcac cgtttgtatc tttgagagcg tcaaacaaac
     8161 ctccagttat ggaataagga ggccttctgt tagataatac tttagctttt atttgaagtg
     8221 ctttttttct ggcttcatca tcgtcgaatg gaagtaattc tctgcttcga actttccagg
     8281 catcgaacat tggagtaaaa ggaaaatttt gagaaggtat caaaatcatt ttatgatttc
     8341 caaatcttcc atcttcaatt agtcttaaat tggcttccca tactgcaata gtacccgttc
     8401 cacttcctat agcctgaaaa taataatcag ggattcggcc tattgtattt actgcagaaa
     8461 gtaatgttgt tgccattccg tctcttcgag ccacattttt tgcaccacct tcttcaaaaa
     8521 acattggaga tttgcaaatt ttgttagaaa gatctatggc atcaaaataa tctgtctgag
     8581 aaggtgtgca gacaatttta acacatttgt ttaaaggctt ttcaaaccac agtgcgtcca
     8641 aattatcttc tggaataact actactaatg gaattttatt gtcagaacag acttttgcaa
     8701 aggctcttgc tgtattaccc gctgaagcaa ctaccatgat tttattatta tcaggatgga
     8761 atcgtgcaca aacagaataa gcctctgttt ccttaaagga acatgtagtc atatttgctc
     8821 ctatttcagg ccaatagcca ctgaaactga taaaaagatt gtccaatccc agtctattgg
     8881 caagcccgtt gctacgataa gttattgtcg aagcagaatt ttcaagcgtt ctacatattg
     8941 gaagccatga agcgtatcta taaaaaccgt aatcattatt attcaattca aactttttat
     9001 tgggataaac agatcttaga agagaaggag taggagattc tggatctgag aggacccatc
     9061 ctgcatcatc gaaaactctt cctgtagaga tattcattaa tttgtagtta ttcattaatg
     9121 tggttaatta tttatatgtt tattaaatta aatgttcaga aacaacttgt gcaatatcta
     9181 cagttttttt gctgttaaca ggtgaaaatg ttttaagaca aagaggacat gcagtaacca
     9241 atgtgtcagg gttgttagta taaagcattg aaagtgaatg ttctgttatc ctctttcttc
     9301 tttcatactg cattgtaaga ctgccaagac tcccaccaca gcagatactt tcaccttttt
     9361 cttcgggaga gtccttaaga ataccaatag aggatagtaa ttctctcggt tcatcatata
     9421 tgttagaccc tcttccaagt tcgcacggat cgtgaaatac aaaactttca tgaactctgt
     9481 caaccttaat ttttccacta ttaagcaagt cattgatata cactgagtga tgaataattt
     9541 ctacaccttg aagattgtac tcttctctaa atactttata acagatagga catgaaagaa
     9601 gaagagttgt acaacctgag tttaaaatta tatctttatt cttgtcaatt agttgacttg
     9661 cacctttaaa atctcctgat aacataagcg gtctgccaca acatattgaa ccgtcagcat
     9721 ccataaaatt gtattctatt ccagcctttt ccagaatttt gaatagtgac aaataaattg
     9781 caggagcaag atgtgtcata catcctgcgt aataaagaat cttctcttta ttatgtttta
     9841 cattttgaag agacaaatag gaaaaatcat gatctcctgt atcattgaaa gattgcctgt
     9901 aaagcagttt caactgtgtt gaattaatcc ctacagggca tacttctaca catttattac
     9961 acataaggca tttttcagcc ccttccagtg cattctctct ctttcgcaat aatcttattg
    10021 tatagactgt agaaaattta atatctccga gatttacgcc cattggacag gcatcaatac
    10081 aaattccaca tctcgagcag gagtatattt ctgactctgc ataaccgttt ctgaaactgg
    10141 ttgccttaat accggcattt ctcaaaaaaa tcaataaaat ttctgtcgga atatgcatat
    10201 atcttgaaaa aggaagtgca aaaagaaaaa cccccagggc tattgaataa gaccaccata
    10261 taggtaatgc attatcggga ttggaaacaa atccgtgaac aagatgattt attgattttg
    10321 tcaaaaaact tccaccactt atatctgctg taaagctttc tgctaaaagc cttaagggga
    10381 aaatagccca aagagagtaa agagcaacag tatcaccaat tttaagtttg gttgttcttc
    10441 tcattcctaa agcccgtgat ctgaaacgct tgaaaattgc aagtgcgatg cctgacagta
    10501 ccatcagaag aaataggtcc ataagaaaga aaaagaaggc acctctaaga gtttgttgag
    10561 tctccatgac aaaatacctg aaaaaaacag gataatagag tattccgttt cgttgaggag
    10621 tataaagcac aacttctatg tgtccaacta caatgagcat aaaccatccg aaagcaatgc
    10681 tgaaatgcat atatccaagt aatggatttc ttttgaatat ttttatatgc aacaggcaat
    10741 cagtaatgac atctttgata gttttaaaaa gttgcttggg ttttagagat ttcagtagag
    10801 agatttgttc ctcatgagtg agggatataa taatcctggc tgttcctaca agaagataca
    10861 aaataatgaa gaccattcca agcgtaaagg ggagtacaaa agggtcataa ccaaataaaa
    10921 acctttcctt cattgcagtc cctccatata aact
//
LOCUS       DAOTQL010000046        10921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5445_length_10921_cov_5.017854, whole genome shotgun sequence.
ACCESSION   DAOTQL010000046 DAOTQL010000000
VERSION     DAOTQL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5445_length_10921_cov_5.017854"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="P5293_04090"
     CDS             complement(<1..222)
                     /locus_tag="P5293_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49116.1"
                     /translation="MGKNKFLEQIPAAAISGNCLDGIVVIINVTGNNILFRYLENLYP
                     QSNYKIYYTEPGIDQLRETIEQCGQFDLKS"
     gene            complement(215..2515)
                     /gene="topA"
                     /locus_tag="P5293_04095"
     CDS             complement(215..2515)
                     /gene="topA"
                     /locus_tag="P5293_04095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRR49117.1"
                     /translation="MGANLVIVESPAKAKTIEKFLGNEYTVKSSYGHIRDLSKKNLGI
                     DIENGFKPTYLISEDKKDIVNELKKLAKKADTVWLASDEDREGEAIAWHLKDVLELPD
                     CKVRRIVFHEITKDAIIHAVENPRNVDMNLVMAQQARRVLDRLVGFELSPVLWKKIKP
                     KLSAGRVQSVALRLIVEREREIQAFSAKSQFKIEGIFNPEGSKTTVKVLAELNEKFDT
                     EKEARELLEKCIKANFNISSIEEKELKRSPSPPFTTSTLQQEASRKLGFSLNQTMRAA
                     QNLYEAGLITYMRTDSTNLSKVALTAAKQTITEMFGKEYSKTRQYETKTKGAQEAHEA
                     IRPTYLNNVNIDAGVQEKKLYSLIWKRTIASQMSDAVIQRTEINIGSDSFTQKFIASA
                     EKIKFDGFLKVYLESADDEDNAGDAFQKLPSLKTGQRMNALTIKAVEKFSQKPPRYTE
                     ASLVKKLEELGIGRPSTYAPIISTIIMRGYIVKDSRPGQERACREIILENGKVSAKVL
                     KETWGAEKNKLFPEDIGIVVNDFLLENFESIMDYNFTAKVEEDFDTIAAGKLVWNEVI
                     EDFYRPFHKQVEKTLEISRPNAERKLGKDPKSGKNLYVRIGRFGPLAQIGDNDDPEKK
                     FVSLAKGQLIETITLEEALKLFELPRVVGSYKGKEIVSGIGRFGPFLKYDNKFISLGK
                     EKDPRTVDLENSIEIIEEHYKKEKEKIIKDFPKERIQILNGRFGAYIKRGKDNFKIPK
                     GTDPKSLELEEVLAIIAKGSNGKHHG"
     gene            2625..4976
                     /locus_tag="P5293_04100"
     CDS             2625..4976
                     /locus_tag="P5293_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRR49118.1"
                     /translation="MNQKSRDKVKKWFWIIVISPFVLVVFALLMVGIFTDIPSFEELE
                     DPKNNLATELISQEGEILTTFHIENRSYIAYDEMSPDLINAVIATEDARFYNHSGIDF
                     KSLARVAVKSIILGQRSSGGGGSTISQQLAKTLFPRDTVVSKIPGAATFNLIVSKFKE
                     WITAVKLERNYTKNEIVSMYLNSVFFGSNSYGIRAAASTFFGKHPSQLTIEEGALLVG
                     MVNKPTRYNPVINPDLSLARRNHVLNQMHKYHYIDQATCDSLKALPVKLNYNPQDHNS
                     GTSAYFRDMLRRVMNAEKPQRSRYRQYDDYRADSILWRDDPLYGWLNKNHRADGTKYN
                     LDKDGLKIYTTVNAKMQQYAQEAIEEHLSKDLQPTFNREIKWKTNRPFSNNVSKTLIE
                     SIMNKARKWSDRYRNLKNAGLSEEEIMASFSKPVRMSVFAWNDRKYIDTTLTPDDSIK
                     YYKSFLRAAFMAMEPNTGYVRAYIGGPNYRFFKYDNARQGKRQVGSTIKPFLYTLAMQ
                     EGYTPCYKVVNVPQTFIVGDTTWTPESTDKPEYIGKTVTLKWGLTHSSNNISAYLMKQ
                     FGPEAMVDMCRRLGITSFLDPVVSLCLGPADLTLYEMVGAYNTYPSKGVHIEPIFVLR
                     IEDKAGNILSDFTPRKREAISEQTAFLMVNLMQGVVNEGTANRLRWKYIPEGQLAGKT
                     GTTNDQADGWFMGFVPKLTAGVWVGAEDRQVHFEGLALGGGANMALPIWGIFMKKVLS
                     DPSLGISVKDVFIAPAVYPEGLDCNGSDNELFNGPSENDPFFN"
     gene            4991..5968
                     /locus_tag="P5293_04105"
     CDS             4991..5968
                     /locus_tag="P5293_04105"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002527.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRR49119.1"
                     /translation="MKKVALIYGGDSSESEISVLSGKNISAHIDRKKYNVYEILLKGG
                     DWNVQLEDGRSFPIDKSDFSFLLPVEDSRAQNRINFDIALIMIHGTPGENGMLQAYLE
                     MVGMPHTTSPSIVSAITFNKYATKCFLRDLNIPMAKDIFIRQGEKWNAAEIISKLGLP
                     LFVKPNEGGSSFGITKVKKIDELDKAVNHALEEDSSVLLEEFIEGREMTNGVFEINGE
                     FSRLPVTEIISKNEFFDYEAKYLGACNEVCPAEISNSLSERIIEQGHRIYHHLGCRGV
                     VRMDYIVRGNEIFFLEINTVPGMTEMSLVPQEIKAAGMTLGNFISKLLG"
     gene            5978..6910
                     /gene="prmC"
                     /locus_tag="P5293_04110"
     CDS             5978..6910
                     /gene="prmC"
                     /locus_tag="P5293_04110"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRR49120.1"
                     /translation="MTYSEFIDYLDVKLSDLYALHERRAMAVRLLCHFCGISHYEHII
                     EPFGLISSDKLSELYRAADQLAMARPLQYVLGFCEFSGLNFHVEDGVLIPRPETEELV
                     RWIGEDRDYADIKEEFAIFDAGCGSGCIGIALANLFPNAKVCMCDISDKAIEVSKFNC
                     RTVLNKAKWPRVNIFKYDLLGYMPLENHIQESSLDIIVSNPPYVRQSEKQLMHRNILE
                     FEPSNALFVSDKDPLLFYRVIAERGRKMLKTSGKIYFEINEAFGYETMQLLQSLGYEN
                     IELKKDLNGRDRMVRASSSRYRESRRDDPPHVEK"
     gene            complement(6846..7460)
                     /locus_tag="P5293_04115"
     CDS             complement(6846..7460)
                     /locus_tag="P5293_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRR49121.1"
                     /translation="MKILKIDLNKMAVVIVCITAFCSCSKKLTFSENEMQLIGLDSTD
                     GIMPIYTVDDPADTALLNAVSSDLSINDMQSEYYKILKKRLLATVQDSTNPGVGIAAP
                     QVGVSKRLVAVQRFDKPEEPFELYPNIRLVSLSQDKAWGWEGCLSVPGKRDTVLRSTS
                     VVISYIDDKDFKEKKDTVEGYTAIIFQHEVDHLDGILYTERKKL"
     gene            complement(7492..10137)
                     /locus_tag="P5293_04120"
     CDS             complement(7492..10137)
                     /locus_tag="P5293_04120"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HRR49122.1"
                     /translation="MKKLWFLSLIFIFLTACQKEDDSLQLSTDNINVSSDIYTAQITI
                     STNQSWSITGGDGWASCSPSSGSGTTTVTLTVDKNTNTSSRNTSLTVIAGSKTQTIQI
                     AQKQKDALILTKNLEAFPNSGGSSTIELKSNISYNVTVPVTASWLTLTQTKAMSTYEY
                     SLQVSQNQTLSSRSAKIVFKDKASNLSDTLVVFQDGPDQISLTTKNYYISKEGATINV
                     DLTYNVDYEMSLNSGVDWVSTVPDSKTTSTKSYSFIIARNNSGTDRFANIIFTQKNAP
                     QGKKAIADTIIIKQSSFDGTYVYLNGNNTLASQLPTSGLTSIQNLKIEGKMKSSDFAT
                     IKNSIVNLKNLDIKDLMLDGDSIPSEALMMTTPVSILESIIFPENLEKIGKSAFNSCV
                     FLKSITLPASVKSIGSMAFYGCASLATVTSNITNTFEVSTNVFSGINPSAILYVPSGK
                     LNSYQTTKGWGYDFFKYICEIGTNPQEYLRLDKYTQNSTGLGCRFSVNITATANWSVE
                     KKPSWVTLSSTSGNGGTSILEITVLPYTGTTIRQDSLILKLNNSSVRAILKIRESGIQ
                     YNDGDVVTLQRATTGAGVDIVILGDGFDFDDIVSGKFESKLREAYSHFFNIEPYKSYQ
                     EYFNVYMVYAYSPESGITDLDNTLNTTFGTRYTKAKPSTSMESNYNTAFTYAAYAPIS
                     NISKTLIIMVANSTRYGGTCVLYSDGKAIAMCPTSNSNYPYDFRGVVQHEAGGHGFGK
                     LADEYVNYSETINTDNINKIKQWQGYGHYMNVDLTSNLTNILWKHFIGIQKYAAVGAY
                     EGAFYYSKGVWRPESTSVMINNIKYYNGPSREQIVKRIKLYAGMVYSFEEFMAKDIID
                     NTPATKAQGMYIDPSMLLAPPILIP"
     gene            complement(10165..10881)
                     /locus_tag="P5293_04125"
     CDS             complement(10165..10881)
                     /locus_tag="P5293_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRR49123.1"
                     /translation="MIISCLIVDDEPLALDLVESYVRQTPFLRLVGRCSNAMEVFAFL
                     EKEKVDLLFLDIQMLGLNGIEISKLLKDMTRVIFTTAYEQYAIDGYKVEAIDYLLKPV
                     SYPDFLKAALKAQKLFEISHQNKEESDNNPGLFIKSDYRMFRVDPEDITFIESEKDYV
                     KIHTTSGESIMALMSMKSIEKQLDHNIFIRVHRSFIVNLLKIKCIERNRIMIGENSIP
                     ISDSYKKELMEKINLRILTK"
BASE COUNT         3132 a         1993 c         2200 g         3596 t
ORIGIN      
        1 acttttcaaa tcaaattgtc cgcactgttc aatagtttct ctcaactgat caatgccggg
       61 ttctgtgtaa tagatcttgt aattactttg tggataaagg ttttccaggt atcgaaaaag
      121 tatattgttg ccggtgacgt ttatgattac aactatacca tccaggcaat tcccactgat
      181 tgctgcagcg ggtatctgtt caaggaattt gtttttaccc atgatgtttt ccgttacttc
      241 cctttgcaat tatagcaagc acttcttcta attcaagaga tttcgggtcg gtacctttgg
      301 gtatcttgaa attatctttt ccccttttaa tgtatgctcc aaaacggccg tttaaaatct
      361 gtattctctc ttttggaaaa tctttgataa ttttctcttt ctctttttta tagtgttctt
      421 ctataatctc aatggaattt tccaaatcga cagtccttgg atctttctct ttgccaagtg
      481 aaataaattt attgtcatat ttgagaaatg gtccgaaacg accaatgccg cttacaatct
      541 ctttgccctt atatgatcct actactcttg gaagttcgaa aagtttcaaa gcttcttcaa
      601 gcgttattgt ctcaattagc tgtccttttg caaggctgac aaatttcttt tcggggtcgt
      661 cattatctcc gatttgagct aatggtccaa aacgtcctat cctgacgtac agatttttgc
      721 cacttttagg atctttgccg agctttcttt ctgcattggg tcttgaaatt tctaaagttt
      781 tctcaacctg tttatgaaaa ggtctgtaga aatcttcaat tacttcgttc catacaagtt
      841 tacctgcggc tatagtgtca aaatcctctt caacttttgc tgtgaaattg taatccataa
      901 ttgattcaaa gttttccagt aaaaagtcgt taaccacaat ccctatatct tcagggaaga
      961 gtttattttt ttctgcaccc caagtctctt tcaaaacttt tgcagacacc ttcccatttt
     1021 caagtataat ttccctgcaa gctctttcct gacccggtct actgtctttg actatatatc
     1081 cccgcatgat aatagttgaa ataatagggg catatgttga gggtcttcct atgccaagtt
     1141 cttcgagttt cttaaccagg gaagcctctg tatatcttgg aggcttttgt gagaattttt
     1201 caacagcttt aattgtcagt gcattcatcc gttgccctgt cttaagtgag ggaagtttct
     1261 gaaaagcatc cccagcatta tcttcgtcat cggctgattc cagatatact tttaaaaaac
     1321 cgtcaaactt gattttttcg gcagaggcaa tgaatttttg tgtaaaactg tcactgccta
     1381 tgtttatctc tgttctttga atgactgcat ctgacatttg ggatgctatt gtccttttcc
     1441 atatgaggga gtaaagtttt ttctcctgaa caccagcatc aatatttaca ttgttcaggt
     1501 aggttggtcg tatggcttcg tgggcctctt gggctccctt agttttggtt tcgtattgtc
     1561 ttgtttttga atactctttt ccaaacatct ctgtaatagt ttgctttgct gctgttaatg
     1621 caactttcga gaggtttgta gagtctgtac gcatataggt tattaatccg gcttcgtaga
     1681 ggttctgggc agctctcatt gtctggttga gagaaaaccc cagtttacgt gaagcttcct
     1741 gttggagtgt tgaagtagta aatggaggag aaggagatcg ttttaattcc ttttcttcta
     1801 tagatgaaat attgaaattg gctttaatgc atttttcaag cagttcccgt gcctcttttt
     1861 cagtgtcgaa cttttcgtta agttcagcca acactttaac tgttgtttta gatccttcgg
     1921 ggttgaaaat gccttctatc ttaaattgag actttgctga gaatgcctga atctctcttt
     1981 ctctctccac aataagcctc aaagcaactg actgcacccg tcctgcagac aatttaggtt
     2041 ttattttttt ccacaaaaca ggtgagagtt caaaaccaac cagtctgtca agtactctgc
     2101 gtgcctgctg agccatgaca aggttcatgt cgacatttct cggattttca actgcatgaa
     2161 ttatagcgtc tttggtgatc tcgtgaaata cgattctgcg cactttacag tcaggaagtt
     2221 caagaacatc tttcaaatgc cacgctatcg cctctccttc ccggtcttca tccgatgcga
     2281 gccagacagt gtctgccttt ttagcgagct ttttaagctc atttactata tcttttttat
     2341 cttcagaaat gaggtaggta ggtttgaatc cgttttcgat atctattccc agatttttct
     2401 tggaaagatc tctaatgtga ccgtaacttg atttaaccgt gtattcattt ccaagaaatt
     2461 tctcgattgt cttggctttg gcaggagatt caacaatcac cagattagct cccatatctt
     2521 taaatgttta ccctataaat ttacaaagta agaaataaag taaggtatta tccaaatttt
     2581 tatttctaat tttgcaatca gacacatact aacatattct tgtaatgaac caaaaatcaa
     2641 gggataaagt aaagaaatgg ttttggataa ttgtaataag cccctttgtg cttgtagtgt
     2701 ttgcactgtt aatggtaggc atctttactg atatcccctc ttttgaggaa cttgaggatc
     2761 cgaaaaataa tcttgcaaca gaactgatat cgcaggaagg agaaattctt acaacatttc
     2821 atattgagaa cagatcgtat attgcttatg atgaaatgtc tcccgatttg attaatgcag
     2881 taattgccac tgaagatgcc cgattttata atcacagcgg cattgatttc aagagtcttg
     2941 caagagttgc agttaagtcc ataatattgg gacagagaag ttctggtggc ggtggaagta
     3001 ctatttcaca gcaacttgcc aaaacacttt tccctcgcga tacagtggta agcaaaatac
     3061 ccggtgctgc gacattcaat ttgatagtat ccaaatttaa agaatggata acagcagtca
     3121 agcttgaaag aaactacacc aaaaacgaga ttgtaagcat gtatctgaac tcagtgttct
     3181 tcgggagtaa ttcatatggt attagagcag ctgcaagtac tttctttggc aaacatccgt
     3241 ctcagttgac tatagaggag ggagctttat tggtgggtat ggttaataaa cccactagat
     3301 acaatcctgt tattaatccg gatctttcgc ttgccagaag aaatcatgtt ctgaatcaga
     3361 tgcacaaata ccattatatt gaccaggcaa catgtgattc tcttaaagct ttacctgtta
     3421 aacttaatta caatccccag gatcataatt ccgggacatc tgcctatttt agagacatgc
     3481 tgcgcagagt aatgaatgca gaaaagccac aaaggtccag atacagacag tatgatgatt
     3541 acagggctga ttccatactt tggagagatg accctcttta tgggtggctt aataagaatc
     3601 atcgtgctga cgggacaaaa tataacctcg ataaggacgg attaaaaatt tacacaactg
     3661 taaacgcaaa aatgcaacaa tatgctcaag aggctattga agaacatttg agcaaggatc
     3721 ttcaaccgac ttttaacaga gaaattaaat ggaaaacaaa cagaccattt tctaataacg
     3781 tttccaagac ccttatagag tctattatga ataaggcaag gaaatggagt gatcgttatc
     3841 gtaatcttaa gaatgcagga ctttccgagg aggaaataat ggcaagtttc tccaaacctg
     3901 taagaatgag cgtttttgca tggaatgaca gaaaatatat tgatactaca cttactcctg
     3961 atgattcaat aaaatattac aagtcttttc tcagagctgc ctttatggcc atggaaccta
     4021 atacaggcta tgtcagagcc tatatcgggg ggcccaatta cagatttttt aaatatgaca
     4081 atgcccgtca ggggaagcgt caggttggtt caactatcaa accttttctt tatacactgg
     4141 ccatgcagga gggttacaca ccatgttaca aagttgtaaa tgtgcctcag acatttattg
     4201 ttggagatac tacttggacg ccagaaagca cggacaagcc cgaatatata ggcaagactg
     4261 ttactctcaa gtggggtttg actcacagtt cgaataatat ctctgcttat ttgatgaaac
     4321 agtttggtcc ggaagcaatg gtagatatgt gcaggaggct tggaattacc agttttctag
     4381 atcctgttgt ttctttgtgt ctgggtcctg cagatctcac tctttatgaa atggtaggag
     4441 cctataacac atacccgagt aaaggcgttc acattgagcc aatttttgtg ttaaggattg
     4501 aggacaaggc aggtaatatt ctttctgatt ttactccaag gaagcgggag gctataagtg
     4561 agcagactgc ttttctgatg gtaaatctaa tgcagggtgt tgtaaatgag ggaactgcca
     4621 atagattgag atggaaatat atccctgaag gacaacttgc aggtaagaca gggaccacaa
     4681 atgaccaggc agatgggtgg tttatggggt ttgtacctaa acttacggcc ggtgtttggg
     4741 ttggagcaga ggacaggcaa gttcactttg aaggtcttgc tcttggaggg ggggcaaaca
     4801 tggctcttcc tatatggggt attttcatga aaaaagttct ttcagatccc tctttaggta
     4861 tatcagtcaa agatgtcttt attgctcctg cagtttatcc agaagggtta gactgtaatg
     4921 gtagtgataa cgaactgttt aatggacctt cggaaaatga cccgtttttc aattaattaa
     4981 tatcaataac atgaaaaaag tcgcactcat ctacggtgga gattcttcag aatcagagat
     5041 atctgtattg agtggtaaaa atatatctgc acatatcgat agaaaaaaat acaatgttta
     5101 tgagattttg ttaaaaggag gagactggaa tgtgcagtta gaagatggcc gtagttttcc
     5161 aatagacaag tctgattttt cctttttgct tcctgtggag gattcaagag cacagaacag
     5221 aattaatttt gatattgctc tgataatgat tcacggtaca ccgggtgaaa acggaatgtt
     5281 gcaagcctat cttgaaatgg tagggatgcc tcatacaaca tctccttcaa tagtatctgc
     5341 cattactttt aataaatatg ccaccaagtg ctttttaaga gatcttaata ttcctatggc
     5401 aaaagatata tttataaggc aaggtgaaaa atggaatgct gcagaaatta tcagtaaact
     5461 tggacttccg ctttttgtaa aacctaatga aggtggaagt agttttggca tcacgaaggt
     5521 taagaagata gatgaacttg acaaggcagt aaatcatgcc ttagaagaag acagctctgt
     5581 tttgctcgag gagtttattg aagggcgaga aatgactaat ggagtttttg aaattaatgg
     5641 agagttctca agattgccgg ttactgagat tatttcaaaa aacgagtttt ttgactacga
     5701 agctaaatac ttaggggctt gtaatgaggt ttgtcctgca gaaatttcaa attctctttc
     5761 tgaaagaatt attgagcagg ggcacagaat atatcatcat cttggatgta gaggtgtagt
     5821 cagaatggac tatatagtca gaggaaatga gatttttttt cttgagataa atactgttcc
     5881 ggggatgacc gagatgagtt tggtccctca ggagataaag gctgcaggga tgactttagg
     5941 taattttatt tcgaagttac ttggttaggt taaacagatg acttattccg aatttattga
     6001 ttatcttgat gtcaagctgt cggatctcta tgcattgcat gagaggcgtg ccatggctgt
     6061 cagacttctt tgtcactttt gtggtattag tcactatgag catataatag agccttttgg
     6121 tttaatttct tccgacaaac tttccgaact ttatagagct gctgatcaac tggccatggc
     6181 acgccctctc cagtatgttt tgggtttttg tgagttttca ggattaaact ttcatgttga
     6241 agacggtgtt ctgattccaa ggcctgaaac ggaagagtta gttaggtgga taggagaaga
     6301 cagagattat gcagacataa aggaagagtt tgcaattttt gatgcaggat gtggttcggg
     6361 ttgcataggg attgcattgg ccaatttgtt tccaaatgcc aaagtttgca tgtgtgatat
     6421 ttctgataaa gcaattgagg tgtccaagtt caattgcaga acagttttga ataaggcaaa
     6481 atggccgagg gtgaatattt ttaagtatga tcttcttggt tatatgccat tggaaaatca
     6541 tattcaagag tcttctcttg atatcattgt aagtaatcct ccttatgtca ggcaatctga
     6601 gaaacagctc atgcacagga atatcctgga atttgaacca tcaaatgcac ttttcgtaag
     6661 tgacaaagac ccgctccttt tttacagagt aatagcagaa agaggcagga agatgctcaa
     6721 aacttcagga aagatctatt ttgagatcaa tgaggctttt ggctatgaaa ccatgcagct
     6781 gcttcagtcg ctcggctatg aaaatattga gttgaaaaaa gacctgaacg ggcgtgacag
     6841 aatggtcaga gcttcttcct ctcggtatag agaatcccgt cgagatgatc cacctcatgt
     6901 tgaaaaatga tcgcagtata accttcaaca gtatcctttt tctctttgaa atccttgtca
     6961 tcgatgtacg agattactac acttgtagat cgtagcacag tatcgcgttt cccgggaact
     7021 gacagacatc cttcccaacc ccatgctttg tcttgagaga gactgactaa cctaatatta
     7081 ggatataact caaacggttc ttctggtttg tcgaatcttt gtacagcgac aagtctttta
     7141 cttacaccaa cttgaggtgc agctattcct actccgggat tggtactgtc ttgcactgta
     7201 gccagtagtc tctttttgag aattttataa tattcacttt gcatgtcatt gattgagaga
     7261 tcagacgaaa cagcatttaa aagagctgta tcggccggat catctactgt atatatcggc
     7321 attatgccgt cagttgaatc aagcccgatc aattgcatct cattttcact gaatgtcaat
     7381 tttttggagc aggaacaaaa agctgtaata caaacgatta ctactgccat tttatttaag
     7441 tcaattttta atattttcat agaataaaat atttagtact acaatgattt atcacggtat
     7501 caaaattggt ggagcaagca acattgaagg atcgatgtac ataccctgag cttttgttgc
     7561 cggagtatta tcaataatat cttttgccat gaattcttca aaagaataaa ccataccggc
     7621 atataatttt attcgtttga caatttgctc gcggcttggg ccattatagt atttaatatt
     7681 gttaatcata acacttgtgc tttcaggtct ccatacccct tttgagtagt agaatgctcc
     7741 ctcataggct ccgaccgcag cgtatttctg aatacctata aagtgtttcc acagaatatt
     7801 ggtcaaattg cttgtcagat cgacattcat ataatgtcca tatccttgcc actgtttaat
     7861 tttatttata ttgtcagtat ttattgtttc ggagtagttt acgtattcat ctgcaagttt
     7921 gccaaaacca tgcccgccag cctcgtgctg cacaactccc ctgaaatcat aaggatagtt
     7981 agaattggag gttgggcaca tggctattgc cttaccgtct gaatatagga cacaagttcc
     8041 gccatacctt gtactgtttg ccaccattat aattaatgtt tttgaaatat tagatatagg
     8101 agcgtaagca gcataagtaa atgcagtatt ataattactt tccatacttg ttgacggttt
     8161 agccttggta tatcttgttc caaaagtagt atttagcgtg ttatctaaat cagtaattcc
     8221 gctctctgga gaataggcat aaaccatata aacattaaaa tattcctgat aacttttata
     8281 tggttcaatg ttgaaaaagt gtgagtaggc ttcccgcagt tttgactcaa acttgccgga
     8341 aacgatgtca tcaaaatcaa aaccatcgcc aagaattact atgtctactc cggcacctgt
     8401 agtggctctt tgtaatgtaa caacatctcc atcattatat tgaatccctg attcccttat
     8461 cttgagtatt gctcttaccg aactgttgtt taatttcaga ataagagaat cttgtcttat
     8521 agttgttccg gtatatggga ggacagtaat ttcaagaata gatgttcctc cattgccaga
     8581 ggttgaacta agagtcaccc acgaaggttt tttctcaacg ctccagtttg ccgtagctgt
     8641 tatattgaca gagaatctac accctaagcc ggtggagttc tgtgtatatt tatcgaggcg
     8701 taaatactcc tgagggtttg ttccaatctc gcatatatat ttgaaaaagt catatcccca
     8761 gccttttgtg gtctggtagg agttcagttt accagatggc acatatagga tagctgaagg
     8821 atttatccca gaaaaaacat tagtgctgac ttcaaaagtg tttgtaatat ttgatgtaac
     8881 tgtcgccaga gaggcacacc cataaaaagc cattgatcca attgacttga cagatgccgg
     8941 gagtgtaata cttttaagaa atacgcagct gttaaaagcc gattttccta tcttttcaag
     9001 attttcagga aagataatag attccaaaat ggaaacaggc gtagtcatca taagagcttc
     9061 tgatggaatc gaatcaccgt caagcatcaa atccttaata tctaaatttt taaggttgac
     9121 tatgctgttt tttattgtgg caaaatcaga acttttcatt ttaccctcta tcttaaggtt
     9181 ttggattgag gttaatccgc ttgtgggtaa ttgggaagca agggtattat tcccgttgag
     9241 ataaacataa gtgccatcaa aggatgactg tttaattatg atagtatctg caatagcctt
     9301 tttgccttga ggagcatttt tctgagtaaa gattatattt gcaaatctgt ctgtcccact
     9361 gttatttcgg gcaataataa atgaatatga ttttgtagag gttgttttgg aatcaggaac
     9421 agtggaaacc cagtccactc cactgttgag agacatttca taatctacgt tatatgtgag
     9481 gtcaacatta attgtagcgc cctctttgga aatataataa ttctttgtag tcagggatat
     9541 ttgatctggc ccgtcctgga acacaacaag tgtgtcactc agattagagg ccttgtcttt
     9601 gaatacaatc ttagcagaac gacttgaaag tgtttgattt tgacttacct gcaaggaata
     9661 ttcatatgtt gacattgcct tggtctgggt gagtgttagc caactggctg ttacaggtac
     9721 tgttacgtta tatgaaatat tacttttgag ttctattgta gagcttcctc ctgagtttgg
     9781 aaacgcctcc aggttctttg taaggataag tgcatccttt tgtttctgag caatttggat
     9841 ggtttgcgtt tttgaaccgg ctatgactgt cagacttgtg ttcctggaag atgtattggt
     9901 atttttgtcg acagtaagtg tgactgtagt tgtgcctgat cctgaactcg gagaacagct
     9961 ggcccatccg tctcctccgg ttatcgacca ggattgattg gtagagatgg taatttgcgc
    10021 tgtatagatg tctgatgaga cgttaatgtt gtctgtagaa agttgtaatg aatcatcctc
    10081 tttctggcag gcggttaaaa aaatgaagat caaagagaga aaccaaagtt ttttcataag
    10141 gtttaaggtt attttagaat aagtttattt tgtcaggatt cttaaattga ttttttccat
    10201 taactctttt ttgtaggagt cggagatggg tattgaattt tctcctatca taattctgtt
    10261 tctttcaata catttgatct tgaggagatt gactatgaag gaacggtgaa ctcttataaa
    10321 gatattatga tcaagttgtt tttcaattga tttcatgctc atgagagcca taatactttc
    10381 accactcgta gtatggatct tgacataatc tttttcgctt tcaataaatg taatatcctc
    10441 cggatcaact ctgaacattc tataatcact tttaatgaat aggcctggat tgttgtctga
    10501 ttcttctttg ttctgatgac ttatctcaaa tagtttttga gccttcagag cggccttaag
    10561 aaagtcagga taacttacag gcttaagaag ataatcaata gcttctactt tgtatccatc
    10621 gatagcatat tgttcatatg ctgttgtaaa aatcactctg gtcatgtcct taaggagttt
    10681 tgaaatttca attccgttta gtccgagcat ctggatatca agaaaaagca gatcaacttt
    10741 ttctttttcc agaaaagcaa aaacctccat ggcattagaa cagcgtccaa ccagtcttaa
    10801 aaaaggtgtt tgccggacat aactttccac aaggtcaaga gcgaggggct cgtcatctac
    10861 tatgaggcaa ctgataatca tgcctcaaat ataactataa atttataatt aacagtgaaa
    10921 t
//
LOCUS       DAOTQL010000047        10794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5510_length_10794_cov_6.094608, whole genome shotgun sequence.
ACCESSION   DAOTQL010000047 DAOTQL010000000
VERSION     DAOTQL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5510_length_10794_cov_6.094608"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..786
                     /locus_tag="P5293_04130"
     CDS             13..786
                     /locus_tag="P5293_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR49124.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLSASLAVMERKVLLVDADPQAN
                     STSGFNFDPDSNSKMTVYEVVIGKVNIKEILLDTEISGLKLAPSHINLVGAEFEMLNI
                     PDREMVLKKSLEEIKNDFDFIIIDCSPSLGIITINALTAADSVLIPVQCEYFALEGLG
                     KLLNTIKLVQNRLNRSLQIEGFLLTMFDARLRHANLVVDEVRNHFNEMVFNTVIQRNV
                     KLSEAPSFGKPVILHDAVSSGTNNYLNLAKEILKKNAQQ"
     gene            787..1710
                     /locus_tag="P5293_04135"
     CDS             787..1710
                     /locus_tag="P5293_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRR49125.1"
                     /translation="MSAMKKPALGRGLGALISDIDNMPFNTRVEIPESQVTPASIMEI
                     SMDKIIANPYQPRSTFDEDSLEELADSIKTLGVIQPITVRTTDDDKYQIISGERRYRA
                     ALLAGLETIPAYIRKADDQGMLEMAIVENIQRENLDAIEIAQSFQRLIEECNLTQEDM
                     ANRVGKKRATITNYLRLLRLPPEVQLAIRAKAISMGHAKALLSIEDSKKQIKLCNQII
                     EHDLSVRQIEDKIRKLASENKQKSEPQDSHDDPSVDEAYIHLLDIFGRHLDNNSFFIK
                     RGENGKGSVTIHFNGEEELFNFLNLLEQTNL"
     gene            1717..2619
                     /locus_tag="P5293_04140"
     CDS             1717..2619
                     /locus_tag="P5293_04140"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRR49126.1"
                     /translation="MSRKFLISVIVMLISFPLSAQITGRLNTAGPPGANLFQQQTTTK
                     NDSTEYENTPKVPRYTIKRYFKSLAHKDSMRINHMWMGSLVLPGTAQIYNKQYWKLPI
                     VYGGMAALIYKGYSSNGNDQEKFYIGAAMLHYATIMDGLVSYKYYKPILPARASLYSA
                     MLPGLGQAYTGNYWKIPIIYGGLITCGYFINFNNIQYQKYKNLYIEAYNDPQSEAATK
                     YSLESLDYFKTAYRRYRDYSILAGILVYALNIIDANVFAHFTDFDVSDDLSASFAPVI
                     IHPLNNQFANNLSPTFGLQVNLNF"
     gene            2621..4240
                     /locus_tag="P5293_04145"
     CDS             2621..4240
                     /locus_tag="P5293_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRR49127.1"
                     /translation="MKIRTLFLVLLTIVLTSELSYGQLLSKRATRKDLLIEIEHLKKT
                     VDSLKNELNGSAIELSDTTTLNDTINPGAIRFFEGEDFMNATVSTDSLLSLYYIQKQF
                     STDTDLNIDLDSAKLTTDIPDSVFIYRLKAMNSFIPLPYNDIVRNHIIFYTQKIPNKI
                     DLILGMSAYYLPIFEEIIDQYDLPKELKVMAVIESALNPNACSRVNAKGLWQFMYRTA
                     LQYNLKINSFVDERLDPIASTHAACKYLKDSYTIFGDWSLAIASYNCGTGNVNKAIRR
                     AGSRDFWKIYPYLPKETRGYVPSFVAALYTLNYYKEHKIVPRPISMPAHVDTFLIHKP
                     LHFGQISEVTGVSVEELQNLNPQYIKQIIPGSPEGMVLNLPYQYTAAFVNNEDSIYSY
                     KDSLYFNPIIFNTGSDKRSYTASATSARETGKTYTHIVRKGETLGGIAMKYGVKLSDL
                     QKWNHTKSRINIGQKLIIHGSSKGNDEFYSSTPTTKSGGYIYYTVKKGDTLWEIAKKF
                     DGVTLNDILELNGLSKESKIFPGKKLKIKRG"
     gene            complement(4261..6216)
                     /locus_tag="P5293_04150"
     CDS             complement(4261..6216)
                     /locus_tag="P5293_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRR49128.1"
                     /translation="MRRMIASLLLLTGSWVSYVEIVRGQELPDKVTEMLIRSVEESGV
                     MPDESGIEEILDYYRELLHFPLNINNAGREDLERLIILTDWQVEAILSYKEEFGNLLS
                     LNELYNIPGLPSQLVSIIIQFLTTDTESSKNKYSAKDLCNNLGFEMISRVKCLIEKEK
                     GFKPISKSEFEKHPDSRYLGPRGQLYSQFKVESMNHLNCAVTLERDAGEKGVDYKSIN
                     LSLINTGCIEKVIIGDYSARFGQGLVLWNTFAMNSYSDSKGLLKYPVGISPYNSTDEN
                     RSLRGAAVTFRAEKWRFTLLVSQRKYDARVVDGTYTSLLKTGLHNTKTTLERKGTLGG
                     SLIGSNLTWSGNNIRVSQTSAIYRYSMPYGGRDSVLRKRDLSMDRYQGNFSLDGYWTL
                     KKVRFFGEVAADHIGSFAAIAGALYSPSNRLETALVLRNYSEEYRAPFASPIARNLSI
                     GGERGGRICSTFSIGKYWKTAVTFELLKDYYNLTLYATKSIEEKGISTSIKYNWSDQR
                     HCFRYNHSFRISSKFTACYRFDFNFFTGKETDIGFHLFGETVFKAFSGKFDASVRVAY
                     FNVPVWETRVYVYERDALHNFSIPFYYGEGIRCYLNVHIAPLKKGDLWLRIASTRYLD
                     RNENGEGITLISCPSKSEAKLQLRLSL"
     gene            complement(6253..6765)
                     /gene="purE"
                     /locus_tag="P5293_04155"
     CDS             complement(6253..6765)
                     /gene="purE"
                     /locus_tag="P5293_04155"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRR49129.1"
                     /translation="MNPKVSIIMGSTSDLSVMKQAAEFLDEMEIPFEINALSAHRVPE
                     MVMEFAKKAYGRGIRVIIAGAGGAAHLPGIIAAFTPVPVIGVPIKSSNSMDGWDSVLS
                     ILQMPSGIPVATIALDGAKNAAILATQILATGDEKLMEKVVKFKSELAGKITKANEEL
                     KRVEYRFKVG"
     gene            6833..7372
                     /gene="hpt"
                     /locus_tag="P5293_04160"
     CDS             6833..7372
                     /gene="hpt"
                     /locus_tag="P5293_04160"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRR49130.1"
                     /translation="MERIKLHDKNFRLFIKNEEIEQSIKTVADQLNNDFKNVERPLFL
                     AILNGSFMFAASLLKNIDFECEIAFVRLSSYNGTSSSGNVKHILGFDKSLEGRNVIIV
                     EDIIDSGETIEEINKILYEANVKDKKICTMLLKPDAVKKDLKIDYAAMRIPNDFIVGF
                     GLDYNELGRQYRDIYILEQ"
     gene            7384..7971
                     /locus_tag="P5293_04165"
     CDS             7384..7971
                     /locus_tag="P5293_04165"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRR49131.1"
                     /translation="MKFYIILGPPGAGKGTQSALLAKKYSLLHISTGDMLRREIEAGT
                     ELGIQVKQTIESGKLVDDETMLKLIKKIISEPQEGIKGFILDGFPRTLPQAEALDKML
                     SERKDSISAVIYLDIDEDIIFQRISYRAKMEDRKDDMERSTIENRIVQYHTKTEPLIV
                     YYSNKGIFYRIKGDKSIEENFKEICSIIDKIEYKR"
     gene            7968..8960
                     /gene="obgE"
                     /locus_tag="P5293_04170"
     CDS             7968..8960
                     /gene="obgE"
                     /locus_tag="P5293_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRR49132.1"
                     /translation="MSSSNFVDYIKIFCQSGNGGKGSSHLRREKYIPKGGPDGGDGGR
                     GGHIILKGDAQTWTLLQFKYRKHIRAGHGEAGSGQLRHGADGKDIILNVPLGTVAKLA
                     ETGEVLFEITADGEEKILVKGGRGGLGNNNFKSATNQTPRYAQPGEPGIENWYILELK
                     ILADVGLVGLPNAGKSTLLSTVSAARPKIANYPFTTLEPNIGIVAYRDNKSFIMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSILLFTVPVDSPDILKEYHVLLDELKCYNPELLDK
                     QRILAITKSDMIDDELEREIQKHLPRKVRHIFISSLSGKNISKLKDMIWESLNN"
     gene            8964..9572
                     /locus_tag="P5293_04175"
     CDS             8964..9572
                     /locus_tag="P5293_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRR49133.1"
                     /translation="MFEKIIDLDKSLFLILNHLTNPFLDKVMVLASEKYTWLPLYIVI
                     AFFIFFKIENGGTIRPRPFKPAFLILAGAFLTFALTDIISASVIKDLVQRLRPGHDPE
                     IGGLARLLDGKGGMYGFVSSHAANVFGLATFTTLCFKKKWYAFFIFFWAILVSYSRIY
                     VGRHFPLDVICGAMLGFLIGWMIYKFKNLIAERINNSHTANN"
     gene            9579..10559
                     /locus_tag="P5293_04180"
     CDS             9579..10559
                     /locus_tag="P5293_04180"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HRR49134.1"
                     /translation="MIYIIDNNTDPYWNLAAEEYLLKNMTQPVFRLWQNDNAIIIGMH
                     QNALAEINIDYVNKNGIKVVRRLSGGGAVFHDMGNLNFTFIDKRERSEDSSAMFARFT
                     KPIIHALEKLGINAALEGRNDLVIDGKKFSGNAIAVYKDRILQHGTLLFSSSIKDLSM
                     ALAGRPEKFISKSVKSTSARVTNISDHIKSHMTIDEFILFLENEIAKAENYNRYYYSV
                     DDLKEIEKLRDEKYSRDWWNLCSAPPYKYSKAFQFPGGLVEVYLEVSKGTISDVRIFG
                     SYFFTRETTEIENILKGCKHTKDDILERLQTINLSHYFNNITREEFISLF"
     gene            complement(10575..>10794)
                     /locus_tag="P5293_04185"
     CDS             complement(10575..>10794)
                     /locus_tag="P5293_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRR49135.1"
                     /translation="KLPRYYDGILGVDVVLSLLPVYDNKNVKMMIKIPGDEIVVERHS
                     VKFEDAVVDCVDVLKDILTKYKEKKIKR"
BASE COUNT         3598 a         2115 c         1959 g         3122 t
ORIGIN      
        1 ttataaaaat aaatgggaaa gattattgcg atagcaaatc aaaagggagg agtaggaaaa
       61 actactacag caattaattt gtctgcctca cttgctgtta tggagagaaa agtgcttctt
      121 gtggatgcag atccgcaagc caattccaca tccggattta actttgaccc ggactccaac
      181 tccaaaatga ccgtatatga agtagttata ggaaaagtga atattaagga gatacttctc
      241 gataccgaaa tctccggtct caaacttgct ccttcacata taaaccttgt aggtgcagaa
      301 tttgagatgc tcaatatccc tgacagggag atggttctta aaaaatctct ggaagagatt
      361 aaaaacgact tcgacttcat tattatcgat tgctctcctt ctttaggtat tataacaatt
      421 aacgctctta ctgctgcaga ttctgttttg attcctgtac agtgtgaata ttttgcactt
      481 gagggattgg gtaagttgct caataccatc aagcttgttc agaacagact taacagatct
      541 ttgcagatag aaggatttct tctgacaatg tttgatgcaa ggcttcgaca tgccaatttg
      601 gtagtagacg aagttcgtaa tcattttaac gaaatggtgt tcaataccgt tatccaacgt
      661 aatgttaagc ttagtgaggc tccgtcattt ggtaaacctg taatattaca cgatgcagta
      721 tcttccggca caaataacta cctcaatcta gcaaaagaga ttttaaaaaa gaacgcacaa
      781 caatagatgt cagccatgaa gaaacccgct ttaggaagag gccttggtgc tctcatttca
      841 gacatagaca atatgccttt caatacaaga gttgaaatcc ctgagagtca ggtcacccct
      901 gcgtctatta tggaaatatc catggataaa attattgcaa atccatatca gccaagaagc
      961 acattcgacg aagattctct cgaggaactt gcagattcaa taaagacact gggagtcatc
     1021 cagcctatta ctgtcagaac cactgatgat gacaaatatc agattataag tggagaaaga
     1081 agatataggg cagctcttct ggctggatta gaaactattc ctgcctatat ccgcaaagct
     1141 gatgaccagg gaatgctcga aatggcgatt gtagaaaata ttcaaagaga aaatcttgat
     1201 gccattgaaa tagcacagag ttttcaaaga cttattgaag aatgcaatct tacacaggaa
     1261 gatatggcta atcgagttgg gaaaaaaaga gcaactataa caaactatct cagacttctc
     1321 cggcttcctc cagaggtgca gcttgccata agagcaaaag ccatctccat gggacatgca
     1381 aaagccttgc ttagcattga ggattcaaaa aaacagatta aattgtgcaa tcagattatc
     1441 gaacacgatc tttcagtgcg gcaaattgaa gataaaatac gcaagcttgc ttcagagaac
     1501 aaacaaaagt ccgaaccaca agactcacat gatgatccat cagtagatga agcatatatt
     1561 catctgctcg atatatttgg ccgccatctt gacaataaca gtttctttat caagagaggc
     1621 gagaatggaa aaggttctgt cacaatacat tttaatggtg aagaagaact gtttaatttt
     1681 ttgaatctac tcgagcaaac aaatctataa agctcaatga gtagaaagtt cctcatatca
     1741 gtcattgtca tgttgatatc gtttccactg agtgcccaaa ttacaggaag gttaaatact
     1801 gcaggccctc cgggtgccaa cttatttcaa cagcaaacca ccactaaaaa cgattctaca
     1861 gagtacgaaa ataccccgaa ggttcctcgc tacactatca agcgatattt caaaagtctg
     1921 gctcacaagg actctatgcg aataaatcac atgtggatgg gatctcttgt tctgccaggt
     1981 actgcccaga tctataataa acagtactgg aaattaccaa ttgtatatgg aggaatggcc
     2041 gcattgatct ataaaggata cagcagcaat ggtaatgacc aggaaaagtt ttacattgga
     2101 gcagccatgc ttcactatgc gacaataatg gatggacttg tatcttacaa atattacaaa
     2161 ccaatacttc cggcaagagc ctcactatac tcggccatgc tgcccggact tggtcaggct
     2221 tatacgggaa actactggaa aattcccata atatacggag ggcttattac atgtggttat
     2281 tttattaatt tcaacaatat tcaatatcaa aaatataaaa atttatatat tgaagcttac
     2341 aatgaccctc aaagtgaagc tgcaacaaaa tacagtctcg aatccctcga ctatttcaag
     2401 acagcctaca gaagatatcg tgactactcg attctggcag gaattcttgt atatgcactc
     2461 aacataattg atgcaaatgt ttttgctcat tttacagact ttgatgttag cgatgatttg
     2521 tcagcatcat ttgcaccagt tataattcat cctttaaaca atcaatttgc taataatctg
     2581 tctccgacat tcggacttca ggtaaactta aatttttgac atgaaaatca gaacattatt
     2641 ccttgtatta ctgacaattg tcttaacttc cgaattatct tacggacaac ttctctcaaa
     2701 aagagcaaca agaaaggatc tgctcatcga gatagaacat cttaaaaaga cagtagattc
     2761 attaaaaaat gaacttaatg gctctgccat tgagctttct gacactacaa cccttaatga
     2821 cacaattaac cctggagcaa taagattttt tgaaggggaa gattttatga atgcgacagt
     2881 atctactgac agtctgttaa gcctctatta catccaaaaa cagttttcga ctgatactga
     2941 tctcaatata gatcttgaca gtgcaaaact tacaaccgac atcccggatt ctgttttcat
     3001 ttaccgactt aaggccatga actcattcat cccactcccc tacaacgata ttgtaagaaa
     3061 ccatattata ttttacacac aaaaaattcc taataaaata gatttaattc ttggaatgtc
     3121 agcatattat ctgccaatat ttgaagagat tatcgaccag tatgatcttc ccaaagagtt
     3181 gaaagtaatg gccgtaatcg aatcagccct taatccaaat gcatgctcga gggtaaatgc
     3241 caaagggctc tggcagttta tgtaccgcac tgcattacaa tataacctta aaattaattc
     3301 ttttgtggat gagaggttgg accctatagc ctccactcat gcagcctgta aatatctgaa
     3361 agattcttat acaatatttg gggactggtc acttgcaatt gcatcttaca attgtggtac
     3421 aggcaatgta aataaggcaa tccgaagagc aggatcacga gatttctgga aaatttatcc
     3481 ttatctgcca aaagagacca gaggatatgt tccatctttt gtggccgctt tatataccct
     3541 taattactac aaagagcata aaattgtacc aaggcctatc tcaatgcctg cccatgttga
     3601 tacatttctg attcacaaac ctcttcattt cggtcaaatt tctgaagtta ccggcgtttc
     3661 ggttgaggag ctgcaaaatc taaatcccca atacattaaa cagataatac cggggtctcc
     3721 tgagggaatg gtcctgaatc taccatatca atacactgct gcgtttgtca ataatgaaga
     3781 ttcaatatat tcatataaag attcattgta cttcaatccg ataattttca ataccggctc
     3841 cgataagaga agttatactg cttcggcaac atctgccaga gaaacaggca aaacatatac
     3901 acacattgta agaaaaggag aaacattggg cgggattgcc atgaaatacg gagtaaaact
     3961 atctgatctg caaaaatgga accatacgaa atcgaggata aacatcggcc agaaactgat
     4021 tattcacgga agtagcaaag gcaatgacga attttattca tctacaccta ctaccaaaag
     4081 cggcggatac atttactaca ccgtaaagaa aggtgatacg ctgtgggaaa tcgctaagaa
     4141 atttgatgga gttactctca acgatattct tgaattgaat ggcctttcaa aagaaagcaa
     4201 aattttccct ggcaagaaac tcaaaatcaa aagaggataa actcggatta tcatcaacga
     4261 tcaaaggctc aatctgagtt gaagttttgc ttcacttttt gacgggcagg aaattaaagt
     4321 gatgccttct ccattttcat ttctatcgag atacctggtt gaggctattc taagccacaa
     4381 gtctcctttt ttcaaaggag cgatatgaac gttaagatag catcgaatac cttctccata
     4441 ataaaaagga atagaaaaat tgtgtagagc atcccgttca tacacataaa ctcttgtttc
     4501 ccaaacaggt acattgaagt aggcgactct cactgaggca tcaaacttcc cggaaaaagc
     4561 tttgaataca gtctccccaa agagatggaa cccaatatcc gtctccttgc ctgtaaaaaa
     4621 attaaaatca aatctgtaac atgccgtaaa cttagatgat atcctgaaag agtggttgta
     4681 tctgaaacaa tgcctctgat cactccagtt atatttgatt gatgtcgata ttcctttctc
     4741 ctcaattgat ttagtggcat aaagagtcaa attataataa tccttaagta attcaaaagt
     4801 tacagctgtc ttccaatact ttcctatcga gaatgttgaa caaattctcc cgcctctctc
     4861 tcctccaatt gacaagtttc tggcaatagg agaagcaaat ggcgctctat attcttcaga
     4921 ataatttctt aatacaagtg ccgtttccaa cctgttcgac ggtgagtaaa gtgctccagc
     4981 tattgctgca aatgaaccaa tatgatcagc agcaacttct ccaaaaaatc ttactttttt
     5041 caaggtccag taaccgtcga gagaaaagtt tccttgatat ctgtccatgg aaagatctct
     5101 ttttctcaaa acagagtctc tgcctccata aggcatggaa taacggtaaa ttgcagatgt
     5161 ttgagaaact ctgatgttat ttcccgacca tgttaaatta gagccaatca gtgaaccacc
     5221 caaagtacct tttctttcaa gagtagtctt ggtgttatga agccctgtct taagcagtga
     5281 agtataagtg ccatccacta ctcttgcatc atactttctc tgggaaacca gtaaggtaaa
     5341 cctccatttt tctgctctaa atgtaactgc ggctcctctc aacgaacgat tttcatcagt
     5401 cgaattataa ggagagatac caacaggata tttgagaagg cctttactat ctgaataaga
     5461 gttcatcgca aacgtattcc aaagaacaag tccctggcca aaacgggcag aataatctcc
     5521 aattatcact ttttctatac acccagtatt aattaaagaa aggttaatcg atttataatc
     5581 aacgcctttc tctcctgcat ctctttcaag agtaaccgca cagtttaaat gattcataga
     5641 ctctacctta aactgggaat ataattgtcc tcttggtccc agatatctgc tgtcgggatg
     5701 tttctcaaac tcacttttac taatcggctt aaatcctttt tctttttcta tcaaacactt
     5761 gactctcgag atcatctcaa atccaagatt attacataaa tccttggctg aatatttatt
     5821 tttgctactc tctgtgtcgg ttgtaagaaa ttgaataata atcgatacta attgtgaagg
     5881 caatcctggt atgttgtata attcattcaa ggaaagcaaa ttaccgaatt cctctttata
     5941 tgaaagaatg gcttccacct gccaatcagt caagattatc aacctttcta aatcttccct
     6001 tcctgcattg tttatattaa gtgggaaatg caataactcc ctgtaatagt caagtatctc
     6061 ctcaatccct gattcgtcag gcataacacc actttcttcc acactcctga tcaacatttc
     6121 tgtaaccttg tccggcaact cctgccctct aactatttct acataagaaa cccagctacc
     6181 tgttaaaagc agcagacttg caatcattcg cctcataaat ccctctcctc ataaaaaaac
     6241 agtaaaagca gtctatccta ctttaaaacg atattcgacc ctcttcaact cttcgttggc
     6301 ttttgtaatc ttacctgcaa gttcagattt gaacttaaca actttctcca taagcttttc
     6361 gtcacctgta gctaaaatct gagtcgccag tatggctgca ttcttagcgc catccagagc
     6421 aattgtagcc accgggatac ctgaaggcat ctgcagtatt gaaagaacag aatcccatcc
     6481 gtccatggaa tttgaagatt ttatcggaac accgataacc ggaaccggag taaaagccgc
     6541 aataatacca ggaagatgtg cagcacctcc tgcaccggca ataattactc ttattcctct
     6601 gccataagcc ttcttagcaa actccatgac catttcagga actctgtgag cagatagagc
     6661 attgatttca aagggaatct ccatctcatc aagaaattcc gcagcctgtt tcataaccga
     6721 aaggtctgaa gtactgccca taataatact gacttttgga ttcatcggtg ttttgttaaa
     6781 ttgggttaag acaaaattaa tatttaattt cgcgaacaca aaatcaattc ctatggaaag
     6841 aattaagctg catgacaaaa actttcgttt atttattaaa aatgaagaga ttgaacaaag
     6901 tattaaaacg gttgcagatc aattgaacaa tgattttaaa aatgtagaac gtcctctttt
     6961 tcttgcaatt ttgaatggat catttatgtt tgcagcttct cttttgaaaa atattgactt
     7021 tgaatgtgaa attgcttttg taagactctc ttcctacaac ggcacctctt cttctggcaa
     7081 cgtaaaacac attcttggtt ttgacaaatc tctcgaggga agaaatgtta tcatagtaga
     7141 agacattatt gacagtggag agactataga agagataaac aaaatccttt atgaagctaa
     7201 tgttaaggat aaaaaaattt gcactatgct tctcaaacca gatgctgtaa aaaaggattt
     7261 aaaaattgac tatgcagcca tgcgcatacc caatgacttc atagttggat ttggacttga
     7321 ttacaatgaa cttggcagac agtaccgaga tatatatatt cttgaacaat aaattagaat
     7381 catatgaaat tctatattat tctaggtcca ccaggagcag gtaaagggac tcagtctgct
     7441 ctccttgcca agaagtattc actgcttcac atctccacag gcgatatgtt gagaagagag
     7501 attgaagccg gaactgaact tggtattcaa gttaaacaga caatagaaag tggcaaactc
     7561 gtggatgacg agactatgct caaactgatt aaaaaaataa tatccgaacc tcaagaagga
     7621 atcaaagggt tcattcttga tggattccca cgaactctgc ctcaagctga agctcttgat
     7681 aaaatgcttt ctgaaagaaa agatagtatt tctgccgtga tatatcttga cattgatgaa
     7741 gatataatat tccaaagaat atcttaccgg gctaaaatgg aagatcgcaa agatgatatg
     7801 gagagatcta ccattgaaaa cagaatcgtc caatatcaca caaagacaga gccgttgata
     7861 gtctactaca gcaataaggg tatattttac agaattaagg gagataaaag catagaagaa
     7921 aattttaagg agatttgctc aattatagat aaaattgaat ataaacgatg agcagttcca
     7981 attttgtaga ctatataaag attttctgtc aatcaggtaa tggaggcaaa ggctcttctc
     8041 atctgagaag agaaaaatat attcctaagg gtgggcctga cggaggtgat ggaggcagag
     8101 gaggtcatat tatccttaaa ggagatgcac aaacgtggac gcttctgcaa tttaaatata
     8161 gaaaacatat aagagccgga catggagagg caggtagcgg ccaactccga catggtgcag
     8221 acggaaaaga tattatccta aatgttcctc tcggcactgt tgccaagctt gctgaaaccg
     8281 gagaagtact ttttgaaatc actgctgatg gagaagagaa aattctggtg aaaggaggaa
     8341 gaggaggact gggcaataac aattttaaaa gcgcgaccaa tcaaactcca agatatgcac
     8401 aaccaggaga gccaggaatt gaaaattggt atatactcga gttaaagatt ttagcagatg
     8461 tgggtcttgt gggtcttcca aatgccggca aatcaacact cctctctaca gtttcagctg
     8521 ctagaccaaa aatcgcaaat tatccattca cgactcttga acccaatata ggtatagttg
     8581 catatagaga caataaatct tttattatgg ccgacatccc cgggattatc gaaggagcac
     8641 atgaaggaaa aggcttggga ttaagatttt tgagacatat tgaaagaaac tccatacttc
     8701 tctttactgt tccagttgac agtcccgata tactcaaaga gtatcatgta ctgcttgacg
     8761 aattgaagtg ttacaatccc gagctgcttg ataagcaaag aattctcgcc attacaaaat
     8821 ctgacatgat agatgatgaa cttgaaaggg aaatccagaa acatttgcca cgtaaagtaa
     8881 gacatatatt tatctcttca ctgtcaggta aaaacatctc caaattaaaa gatatgatct
     8941 gggaatcact aaataattag caaatgtttg aaaaaataat agatcttgat aaatctcttt
     9001 tcctaatcct taatcactta accaatcctt ttctggataa agttatggtc ttggcctctg
     9061 aaaaatatac ctggctgcca ttatacatag ttatcgcatt ttttatattc tttaaaattg
     9121 agaatggagg gacaatccgt ccgcgcccat ttaaacctgc ttttctgatt ctggccgggg
     9181 catttctaac atttgcactt acagatatta tctctgcaag tgtaattaaa gatcttgtcc
     9241 agagattaag acccgggcat gatcctgaaa taggaggatt agcaagattg ctcgacggca
     9301 agggaggtat gtatggtttt gtatcaagtc atgctgcaaa tgtatttggt ttggcgacat
     9361 ttacaacact ttgctttaaa aagaaatggt atgctttctt catattcttt tgggcaatat
     9421 tggtaagtta cagccgaata tatgtaggca gacacttccc tcttgacgtt atttgcggag
     9481 ctatgctcgg atttcttatc ggatggatga tttataagtt taaaaattta attgctgaaa
     9541 gaataaataa ctcgcataca gcaaataatt aatatcatat gatatatata atcgacaata
     9601 acactgatcc atattggaat ctggcagcag aagaatacct tctcaaaaac atgactcaac
     9661 cggttttcag actttggcag aatgacaatg caataattat tggtatgcac cagaatgctc
     9721 ttgcagagat aaacattgat tatgttaata aaaacgggat taaggtagta aggcgtttat
     9781 ccggaggcgg tgctgttttc cacgatatgg gcaatctcaa ttttactttt attgacaagc
     9841 gggaaaggtc tgaagatagt tctgcaatgt tcgccagatt tacaaagcca ataattcatg
     9901 ctctcgaaaa attgggtata aatgctgcct tggagggaag aaatgatctg gtaattgacg
     9961 gaaaaaaatt ttcaggaaat gccatagctg tttataagga tagaatactc caacacggta
    10021 ctcttctgtt ttcatcttca atcaaggatc tctcaatggc attggcggga agacccgaaa
    10081 aattcatcag taaatctgta aagtcaacca gcgcacgagt cactaacata agtgaccata
    10141 ttaaaagtca catgacaatt gatgagttta ttctttttct cgaaaatgaa atcgcaaaag
    10201 cagaaaatta taacagatat tattattcag tggatgatct gaaagagata gaaaaattaa
    10261 gggatgaaaa atacagtcgt gattggtgga acctgtgttc agcaccacca tacaaatatt
    10321 ctaaggcttt tcaattccct ggagggttgg tagaagtata tctagaagta tcaaaaggga
    10381 cgatatccga cgttcgtatt tttggaagct atttctttac acgggaaaca accgaaattg
    10441 agaatattct caaaggatgc aaacacacta aggatgatat tcttgaaaga ttgcaaacaa
    10501 taaacctctc tcattatttc aacaatataa caagagagga gtttatttca cttttctaaa
    10561 atatttaatt ataattatct ttttattttt ttctctttat atttagtaag tatatccttg
    10621 aggacatcaa cacaatctac gaccgcatct tcaaacttaa cagagtggcg ttcaacaact
    10681 atttcatctc ctgggatttt gatcatcatc ttcacattct tgttgtcata aaccggcaac
    10741 agacttaaga ctacatctac cccgagaatg ccgtcataat aacgtggaag ttta
//
LOCUS       DAOTQL010000048        10775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5522_length_10775_cov_4.279944, whole genome shotgun sequence.
ACCESSION   DAOTQL010000048 DAOTQL010000000
VERSION     DAOTQL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5522_length_10775_cov_4.279944"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="P5293_04190"
     CDS             <1..1435
                     /locus_tag="P5293_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR49136.1"
                     /translation="FSDDQALHSGINTFPTAAPASSTDWNNAYIYIRKANLLLERADK
                     VPMTDEAKNHYKGIARFFRAFEYANLIKRFGDVPYIDKFLDQSQTDLIYTPRTARNTI
                     VDNIVADLQFAAQNIRTKAAAEANTVNRDVANALLSRVALYEGSIAKYHNNDLTRANA
                     LFTIAKNAANEVMSQNYKLSTNYKDIYTSISLASNTEAILYKYYVKGTFMHCLTAYLN
                     STTPISGPTKDLVESYLCKDGKPIGTSTQYQGDADINSLLANRDTRLADMIGNVLAYK
                     GNPVGGLISSTGYLVKKFNNPSITGNDLTQINMNYTNAPLFWLAEVYLNYAEAAAELG
                     TISQTDVDNTINKLRTRGGVASLNITSIPSDPKKDASVSALLWEIRRERRVELALDGF
                     RTWDLRRWKKISYLDPAVKPDIFKGAKVPAQTTGLLVDANMYIVPYSNISLRIVTEPK
                     HYLDPIPTGVKNLYDLQGVDFPQNPGW"
     gene            1532..2923
                     /locus_tag="P5293_04195"
     CDS             1532..2923
                     /locus_tag="P5293_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRR49137.1"
                     /translation="MFEKNVYIRRREALMKSMKSGLLLFLGNSEVGCNYPSNTYPFRQ
                     DSSFLYFFGLDEPDLAAIIDVENGEEIIFGNDVSMDDIIWMGPQPKMRDKALMVGVNK
                     VMTHESLQDYLVKANKSGRRIHYLPPYRDANKIYLNSVLGIPFSELKSKASVEFIKAV
                     VALREIKKEEEIREMDEASNIGYTMHYTAMKMLKAGMVEQELVGVMEGIAHSQGPMTS
                     FPTILSQNGETLHNHTHHQVLTNGRLLVIDAGAENKLHYASDFTRTLPVSGKFTQQQR
                     DIYTIVSQANALAISLVRPGITYMEVHKAAIRLIAQGLKNLGLMKGDVDEAVNQGAVA
                     LFMPHGLGHQLGLDVHDMEDLGEDYVGYDDNIQRAKQFGLASLRMGKELRPGHIITVE
                     PGCYFIPALIDKWRDEGMYREFINYDALQPYYKFGGIRLEDNVIVTENSCRLLGLSRL
                     PITTDDVEKSMEA"
     gene            3066..6236
                     /locus_tag="P5293_04200"
     CDS             3066..6236
                     /locus_tag="P5293_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR49138.1"
                     /translation="MYVSQQFTVWCKQFALAVILVLASSAIFAQNIRVTGKVVDNSGQ
                     PTVGVSVMIEGTKTGVATDLDGNYSIEAPANGVLIFSAIGMENQRIEIQGRRVINVTM
                     KESAQLLEELVVTALGIKKEKKALGYAVQDIKSDEILKNKQTNVINSLAGKVAGVNVT
                     QSSGSAGVGSTIIIRGGNSASESRDNQPLFVVDGVIYDNSTVNSGNSLTDGVTKNATS
                     FGNRVMDINPEDIENMSILKGAAATALYGSRAADGVVVITTKKGTEGSTRVNFSSKFS
                     YSWVGQTPELQGVYGRGYYNQVGVFSDYTTNSWGNKITGPVYDNIGEFFQGGTVYDNN
                     ISVSGGSSKGNFYLSASNFDQEGIIPKTGFYKTTFRFNGEQKYGKLTVGANASYAVAN
                     TLKTLTAAGLYNGGGNGTMTAVYGWPRSDDMKHYLNEDGSKYRMFEGLQQLADDVENP
                     YWILNKNRLTDYNNRFTGSVNAAFNVVDWFNITYRLGYDNYDNGSFTYIAPGGAVLEK
                     YQKGRLSKSNGKYNYMSSNLMLNFQKSINDFDFGLLLGQAIEDTKRSTWTHWGYDFKT
                     PGTISFANIIDLNKFFTERNMKKRMVSVFGELRANYKNIAYLTVTGRNDWSSTLPKEN
                     RSYFYPSVAGSFLFTELLPKNDILSYGKLRLSWAQVGKDADAYATNTYLWPSQTVSGN
                     FVGAGNNWTGGGPNLKPEIQTSYEIGTELRFLKGRLGLDFTYYNSETKNQLAAPRLCQ
                     STGYIFLTLNSGSVKNQGMELSINATPIDKKDFTWDVTLNLSGNRGKLGDFLPGVGLF
                     YVTDVQIGGVKAASIPNGGYFLGMTGDYWVREKDANNKEIPGGRYVIDEATGLYTTTK
                     VTTNIVGNREPKVIGGLNNSFRYKNLDFSFLLDIRIGGDIYNGTEYYTTMKGLSKRTL
                     QRDAVTVSGVGKASGQPLSYTYERGQNYVINNATYSGAYMIQQYWSSYCDNSYNFITD
                     TNWLRLRSISLTYDFTSMFKKNGFVKGLSANISANNLFVWTNYKGMDPEVSVSGSGTG
                     GSGSMGIDYCGVPSTKNVTFGINLTF"
     gene            6263..8194
                     /locus_tag="P5293_04205"
     CDS             6263..8194
                     /locus_tag="P5293_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRR49139.1"
                     /translation="MKKILHITLSLVFLLSVSSCNEWLDVNVDPDNPSDQSATVEIRL
                     PWIQHYFMYAYGTANMRTSTIAGIITQTSTTSGNGLLAAWDPAQSSCTTIYQNWFIGA
                     AVNLQPMIDKAIKTGSYHYIGAAYCIRAMGFLMMLDLHGEMPYTEALTGKYNPKYDDG
                     KTIYDGCMADLDRAIEYFNMTQKAGSTPLSAGDTWNGGDVQKWIKLCYGVKARYLNII
                     SKKSNYSPDAVLAALEHAPQSNADNTVMKHYNVQGDATNITVGDPYQTNSTWNSVGYG
                     TSQRMTRWYLNLYTNKYTGGSNVIDPRLSKVFPAMMKNIKLDASGKILSYEWARDIGV
                     DMMNSDIRQNSGPMLASYATADKVIKYTIADQAERNSFIASLGSKPYTVSGNDVSVTY
                     RKGSFYVNSTNYKRAGDTVYVNLRSNSLSTSGRSATDTYYYILADQDAVAGTGTFYAR
                     PDSDSDILTYAEMCFIKAEVYLRKNMQSQAHAAYLAGIQAHFNRMQTKLNEWKSKGVV
                     NPDQMPMSDADIAAYMASAAVVQNPANLTMAEIMKQKMIALGFDYQNWNDMRRFNYSA
                     GNIGNFGVVYKDYKRPYEFKATNKMTGTSPTDLTYWFRRFSQSTHESNYNKEQLMASN
                     PLAMQDAIWSDPVWWDIPE"
     gene            complement(8363..9046)
                     /locus_tag="P5293_04210"
     CDS             complement(8363..9046)
                     /locus_tag="P5293_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49140.1"
                     /translation="MKQIINLLSIVLIITALNFTTSCNLANTNNNSRADIRLDTLSMK
                     DSTANCEILIVTPVCDINEFDTCIKNELLRQKDQFIESIVKPEFSPNETHPPFISSFM
                     ASPSSIYSDRKIVSYSFTVFNYMAGAAHPMSSYYSFNFDKKKKARISFSDFFRLTSKA
                     DTTALLDIINKSINREGVSLDSLYELDFNFIKDTVSFNFDCYEIACYAEGIIQSKVAI
                     KDLKPFLKR"
     gene            complement(9063..9893)
                     /locus_tag="P5293_04215"
     CDS             complement(9063..9893)
                     /locus_tag="P5293_04215"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRR49141.1"
                     /translation="MEQEVKYVDIRKILSEGNSTFLQSLPRFVTNIIAGIIKEDELNY
                     IMNKYKDFIGKDFLPEIIDELNLKIEITGTENLPESGRCFFVANHPFGIVDGLILTYI
                     ITQKYGTVKAIANEAFMYIPQLRPLIAAVNVFEGSSREYLQALEEVYNGNDPISHFPA
                     GEVSRKYHRKVEDCDWQKSFISKAISSKRDVVPICFRGRNSNLFIFIYMFRKLFGIKT
                     NIELALLPRELFNKKGKTIRVKIGKPIPWQTFDKTHSHFEWAQIVKKQVYSLWENFTE
                     "
     gene            10135..>10775
                     /locus_tag="P5293_04220"
     CDS             10135..>10775
                     /locus_tag="P5293_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRR49142.1"
                     /translation="MDRTLIPKGYKSPLDIWQTELAIKFIKDTFQVELSSELKLRRIT
                     APLFLQAGTGLNDNLNGVETPVSFKTEGGNGNMAEIVQSLAKWKRFALWRHNFKPGIG
                     VYTDMNAIRPAEITDNIHSIYVDQWDWEKVIKPSDRNVKTLYNCVRHIYSAIKRTQFL
                     LTERFTQLDPFLPDDISFFHAQDLLEMYPDLEPKERENEAARKYGAIFIRGIGG"
BASE COUNT         3358 a         1897 c         2259 g         3261 t
ORIGIN      
        1 tttttctgat gaccaggcgc ttcattcagg aatcaacaca tttcctacag ctgcaccggc
       61 atccagtaca gactggaaca atgcctatat ttacattagg aaagccaatc ttttgcttga
      121 aagggcggat aaggtaccaa tgacagatga ggccaagaat cattacaagg gcattgcaag
      181 gtttttccgt gccttcgaat atgccaattt gataaaacgc tttggagatg ttccttatat
      241 tgacaagttc ctcgatcagt cgcagacaga tttaatttat actccgagaa cagctagaaa
      301 tacaattgtt gacaacattg tagcggatct tcagtttgct gcacagaata tccgaacaaa
      361 agcagctgct gaggccaata ctgtcaacag agatgtggca aatgcacttc tttctagagt
      421 ggctttgtat gaaggctcta ttgccaagta tcacaataat gatttgacaa gagccaatgc
      481 tctctttaca attgcaaaga atgcagcaaa cgaggtaatg tctcagaact acaaattaag
      541 cacaaactat aaggatattt atacaagcat ctcacttgct tccaataccg aagcaatatt
      601 gtacaagtat tatgttaagg gtacttttat gcactgtctg acagcatatt tgaattccac
      661 aactcctatc agcggaccta ctaaggatct tgtagagtca tatctctgca aggatggcaa
      721 gcctatcggt acaagcacac aatatcaagg agatgctgac atcaacagtc tccttgccaa
      781 cagagatacc cgccttgcag atatgatagg caatgttctt gcttataagg ggaatcctgt
      841 tggaggatta atttcttcta cagggtatct ggttaaaaaa ttcaataatc cttctattac
      901 gggcaatgat ctgacccaga ttaacatgaa ttatacaaat gctcccctct tttggcttgc
      961 agaagtttat cttaattatg cagaagcagc tgccgaactg gggaccatat ctcagacaga
     1021 tgtagataat accattaata aattgcggac aagaggggga gttgcttctc ttaatattac
     1081 aagtattcct tccgatccaa agaaagacgc aagtgttagt gccttgttat gggaaataag
     1141 aagagagagg agagttgaat tggcgctgga tggattccgt acttgggatc ttcgccggtg
     1201 gaaaaagatt tcttaccttg atccggcagt aaagcctgac atatttaagg gcgcaaaggt
     1261 tccggcacag acaacaggtt tgcttgtaga tgcaaatatg tacattgttc cgtacagcaa
     1321 tatttctcta agaattgtga ctgaacctaa acattatctt gaccctattc ctacaggagt
     1381 aaagaatctg tatgacctgc aaggtgtaga ttttcctcag aatccaggat ggtaaatagt
     1441 tttcaaaaca aaaaacggag ggtgccttca aaggtgccct ctttttttta actttgttaa
     1501 aattgattat taacaatata aaactaatgt tatgttcgaa aaaaacgtgt atatcagacg
     1561 cagagaggct cttatgaaga gtatgaaatc tggtcttctt ttgttcctcg gcaattcgga
     1621 agttggatgt aattatccgt caaatacata tccttttagg caagattcgt cattccttta
     1681 ttttttcggg ttggatgagc ctgaccttgc ggccattata gatgtggaga acggtgaaga
     1741 gattatattt ggtaatgacg tttccatgga tgatatcatt tggatgggtc ctcagccaaa
     1801 aatgcgtgat aaagccctta tggtaggtgt gaataaagtt atgacgcacg aatctttgca
     1861 ggattatctt gtaaaggcca ataaatcagg gagaaggatc cactatcttc ccccttatag
     1921 ggatgccaat aaaatctatt taaattcagt tcttgggatc cctttttctg agctaaaatc
     1981 aaaagcttca gttgaattta ttaaagctgt tgttgctctt cgagagataa agaaggaaga
     2041 agaaataaga gagatggatg aggcatcaaa tataggttat accatgcatt atactgccat
     2101 gaaaatgctc aaggccggga tggtggagca ggagttggta ggtgtaatgg aaggcatagc
     2161 tcactctcag ggacctatga cttcgtttcc aacaattttg tcacaaaacg gagagacact
     2221 tcacaatcac acacatcatc aggtccttac aaatggaagg ctgcttgtca tagatgcagg
     2281 ggctgagaat aaattgcatt atgcttcgga ttttacaagg acacttcctg tttccgggaa
     2341 gtttactcaa cagcaaagag acatttatac cattgtatct caagccaacg ctcttgcaat
     2401 aagtcttgta agaccaggta taacatatat ggaagttcac aaggctgcaa taaggcttat
     2461 tgcacaggga ttgaaaaatc tcggcctgat gaagggcgat gttgacgaag ccgtcaatca
     2521 gggagctgtg gctttattta tgcctcatgg actgggacat caacttggtt tggatgttca
     2581 tgatatggaa gatcttggag aagattatgt cggctatgac gacaatattc agcgtgcaaa
     2641 acagtttgga cttgcctctt taagaatggg taaggagtta agacctgggc atatcattac
     2701 tgttgaacca ggatgctatt ttatccccgc acttattgac aaatggaggg acgaagggat
     2761 gtacagagag tttataaatt atgatgctct acaaccttac tataagtttg gaggcataag
     2821 acttgaagac aacgtaatcg tgactgaaaa ttcctgtaga cttttaggtt tatcaagatt
     2881 acctattact acggacgatg ttgaaaagtc aatggaagca tgaatgaaat ttccttataa
     2941 tctataatat gaggaagatg taagttaggc caacaagatt cttcatttta attaccgaat
     3001 tttacattat tagtgttaaa tttacatgca tttttttaca ctaattaacc ttaaatatta
     3061 atttcatgta tgtttcacaa caatttactg tctggtgcaa gcaatttgcg ctagcagtta
     3121 ttttggtgct tgcatcaagt gccatttttg cccaaaacat cagagttaca ggaaaggtgg
     3181 tagacaattc cggtcaacca accgtgggtg tctccgtcat gattgaggga accaagacag
     3241 gcgtagccac agaccttgac ggtaattact caattgaagc tcctgcaaat ggtgttttga
     3301 ttttctctgc aatcggtatg gaaaatcaac gtatcgaaat tcagggaagg agggtcatta
     3361 atgtgaccat gaaggaatcg gctcagctat tggaggagct ggtagttacc gcattaggca
     3421 tcaagaagga gaaaaaggcc ttgggatatg ccgtacagga tattaagagt gatgaaatac
     3481 tcaaaaacaa acaaactaat gtaatcaatt cactggcagg aaaagtagct ggtgtaaatg
     3541 tgactcagtc aagtggttct gccggcgtag gttccacaat tattattcgt ggaggtaact
     3601 cagcaagtga atctcgtgat aaccaaccac tttttgtcgt agatggtgtt atctatgata
     3661 actcaacagt aaattcaggg aactcattga cagatggtgt tacaaaaaat gctacatctt
     3721 ttggtaaccg tgtaatggat ataaatcccg aagatatcga gaacatgtcg attctgaagg
     3781 gtgcagcagc tacagctctt tacggttcac gtgctgctga cggtgtagta gttattacaa
     3841 ccaaaaaagg tactgaaggc tcaacaaggg taaacttcag ttctaaattt tcatattcct
     3901 gggtaggtca gactccggaa cttcagggtg tttatgggag aggttattac aatcaggtag
     3961 gagttttcag tgactatacc acaaactctt gggggaataa aattaccggt cctgtgtatg
     4021 ataacattgg ggagttcttc cagggaggca ctgtttatga taataatata agtgtatccg
     4081 gtggttcgag caaaggaaat ttttaccttt cagcatcaaa ttttgatcaa gagggaatta
     4141 ttcctaaaac tggtttctac aagacaacat tccgtttcaa tggagaacaa aaatacggta
     4201 agctgacagt tggagcaaat gcatcctatg cagttgccaa taccttgaag actcttacag
     4261 ctgcaggtct ttataatggt ggaggaaacg gaactatgac agctgtttat ggctggcctc
     4321 gcagtgatga tatgaaacat tatctcaatg aggacggatc taaatacaga atgtttgaag
     4381 gtcttcaaca gctggcagat gatgttgaaa acccttactg gattcttaat aagaacagac
     4441 ttacagatta taacaaccgt tttactggat cagtaaatgc tgcttttaat gtagtggatt
     4501 ggtttaatat tacttatcgt ttaggatacg ataattatga taacggttcc tttacttata
     4561 ttgctccagg tggtgctgtt ctcgaaaaat atcagaaagg tcgtctcagt aagagcaatg
     4621 gtaaatataa ctacatgtcg tcaaatttaa tgctcaattt tcagaagagc atcaatgatt
     4681 ttgactttgg acttttgctt ggtcaagcta tcgaagatac aaagagatct acatggacac
     4741 attggggata tgattttaaa actccaggca ccattagttt tgcgaatata attgatctaa
     4801 acaagttctt tactgagagg aatatgaaga agcgtatggt cagcgttttt ggcgaactta
     4861 gggccaatta caaaaacatt gcttatttaa ccgttacagg tcgtaacgac tggtcttcaa
     4921 ctcttccaaa ggaaaataga tcttatttct acccttctgt tgcaggcagt tttctgttta
     4981 ccgaactgct gcctaaaaac gacattcttt cctatggcaa gcttcgccta tcatgggctc
     5041 aagtaggtaa agatgcagat gcgtatgcta ccaataccta tctttggcca tctcaaacag
     5101 taagtggtaa tttcgtgggt gccggtaata actggacagg aggtggccct aatttaaagc
     5161 cagaaattca gacatcttat gagataggta cagagcttag atttctaaag ggaaggctgg
     5221 gtcttgattt tacatattac aacagtgaaa ctaaaaacca gcttgctgcc cctcgtcttt
     5281 gccaatcaac aggatatata tttcttactt taaatagtgg ttctgttaaa aatcagggta
     5341 tggagctatc aataaatgct actccaattg ataaaaagga ttttacatgg gatgtaactc
     5401 tcaatctctc cggtaaccgc ggcaagcttg gagattttct tccgggagta ggtctatttt
     5461 atgtaaccga tgttcaaata ggaggggtta aagctgcttc aattccaaat ggtggatact
     5521 tccttggtat gacaggcgat tactgggtga gggaaaagga tgccaacaat aaagagattc
     5581 cgggaggaag atatgtgatt gatgaggcaa caggactcta taccacaact aaagtcacta
     5641 ccaatattgt aggtaacagg gaacctaagg ttattggagg tttgaacaac agtttccgtt
     5701 ataaaaactt ggatttttca tttcttctcg atataagaat aggaggtgat atttataatg
     5761 gcacagagta ctacacaaca atgaagggtt taagtaaaag gactctccag cgtgatgccg
     5821 ttacagtatc aggtgtagga aaggcatcag gccaaccgtt gagctatact tatgagcgcg
     5881 gtcagaatta cgttatcaat aatgctacct actctggtgc ttatatgata cagcagtact
     5941 ggtcaagtta ctgtgacaat tcatataatt ttattactga cacaaactgg ctgagacttc
     6001 gttctatctc actcacatat gattttacaa gtatgtttaa gaaaaatggc tttgtaaaag
     6061 gtctttcagc aaatatatct gccaataatc tatttgtatg gactaactac aagggaatgg
     6121 atccggaagt gagtgtttct ggatcgggta caggtggttc aggttcaatg ggtatcgatt
     6181 actgcggtgt gccttctacc aagaacgtga catttggcat taatttaaca ttttaattaa
     6241 cagatttagg aaaaatatga ttatgaaaaa aatattacat ataactttaa gtctggtttt
     6301 tctacttagt gtatcctcat gtaatgaatg gttagatgtc aatgttgacc ctgataaccc
     6361 aagtgaccag agtgctacag tagaaatcag gctgccatgg atacagcatt attttatgta
     6421 tgcatatggt actgccaaca tgcgtacaag tacaatcgca ggaattatta cacaaacttc
     6481 cactacttcc gggaatggtc tcttagctgc atgggatcct gctcagtcaa gttgcactac
     6541 catctatcag aactggttta ttggtgcggc tgttaatttg caacctatga ttgataaggc
     6601 tatcaaaacc ggttcctatc attatattgg agcagcttat tgtataagag caatgggctt
     6661 tttaatgatg ctcgatttac atggagaaat gccttatact gaggctttaa caggtaaata
     6721 caaccctaaa tatgacgatg gcaaaactat ttatgatggc tgtatggctg atttggatcg
     6781 tgctattgag tactttaaca tgactcaaaa ggccggctct acaccactct ctgccggaga
     6841 tacgtggaat ggcggagatg tccagaaatg gattaaatta tgttatggtg taaaggctcg
     6901 ttatctgaac attatctcca agaagtcaaa ctacagtcct gacgccgttc ttgccgcact
     6961 ggaacatgca cctcagtcca atgcagataa tactgtgatg aaacattaca atgttcaggg
     7021 agatgctaca aatattactg taggagaccc ttaccaaact aacagtactt ggaactcagt
     7081 tggatatggt acttcacaac gtatgaccag atggtatcta aatctttata ctaacaaata
     7141 tacaggtggt tctaacgtta ttgatccacg tttatccaag gttttccctg caatgatgaa
     7201 aaacataaaa ttggatgctt cgggcaagat tttaagttat gaatgggcac gagatattgg
     7261 agttgacatg atgaactccg atatccgtca aaatagtggg ccaatgttag cttcgtacgc
     7321 aacagcagat aaggttataa agtatactat tgccgatcag gctgaaagaa attcctttat
     7381 tgcttctctt gggagtaagc catatactgt cagtggaaat gatgtttctg tcacatatag
     7441 aaagggatct ttctatgtca acagcacaaa ctacaaaagg gcaggagaca ctgtatatgt
     7501 aaatttgaga tccaacagtc tgtcaacaag cggaaggtct gctactgata catattacta
     7561 tattttagct gatcaggatg cagtagccgg tacaggtact ttctatgcaa gaccggattc
     7621 agactcagat attcttacat atgccgagat gtgtttcatc aaggctgagg tctatctaag
     7681 gaagaacatg caatcacagg ctcatgctgc atatcttgca ggtattcagg ctcattttaa
     7741 caggatgcag accaagctta atgagtggaa gtctaagggt gttgtcaatc ccgatcagat
     7801 gcctatgagt gatgcagata ttgctgctta tatggcaagt gctgcggttg ttcaaaatcc
     7861 tgcaaatctg actatggccg agattatgaa acaaaaaatg atcgctctgg gcttcgacta
     7921 tcagaattgg aacgatatga ggcgcttcaa ctacagtgct ggcaatattg gcaactttgg
     7981 cgttgtttac aaagactaca agaggcctta tgagtttaag gcaactaata agatgacagg
     8041 aacaagccct acagatttga catattggtt ccgccgcttc agtcagagta cacacgaaag
     8101 taactacaac aaggagcagt taatggcatc aaatcctctt gccatgcaag atgcaatctg
     8161 gagtgatcct gtttggtggg atattcctga ataatttgta tttgatattg tctcattaga
     8221 taaagagggt gtccaaaaag caatttttgg cccctttttt tcatttttta agccccgaaa
     8281 aaatcaaaga aaaaaagcgt taaaaaaaca gcagtgacgt gggggagctg tcatttcgac
     8341 gggatatatt tatttgaaaa agttaccttt taaggaaggg ttttaaatct ttaatagcta
     8401 ctttgctctg tataatacct tcagcgtaac atgcaatttc ataacagtca aagttaaaag
     8461 agacagtatc tttgatgaag ttaaaatcta attcataaag agaatcaagt gatactcctt
     8521 ccctgttgat tgatttgttg ataatatcaa gcagggcagt tgtgtctgct ttacttgtca
     8581 atctgaaaaa gtcactaaag cttattcttg cttttttctt tttgtcaaaa ttgaaagagt
     8641 agtaggatga catgggatga gcagcacctg ccatgtagtt aaaaacagta aaagaataac
     8701 ttacgatttt tctatcactg taaattgatg acgggcttgc catgaaactg ctaataaaag
     8761 ggggatgagt ctcatttgga gagaattcag gtttcactat actttcaata aattgatctt
     8821 tttgtctaag taattcattt tttatacaag tgtcaaattc atttatgtca cacacaggag
     8881 tgacaattaa tatttcgcaa tttgcagttg aatctttcat gctaagagtg tcaagtctaa
     8941 tatcagctct actattattg ttagtatttg ctaagttgca acttgtagta aagttcaagg
     9001 ctgttataat caggacaata cttaggagat taataatttg tttcataata atagttattt
     9061 gtttattctg tgaagttttc ccataaagaa tagacctgtt tttttactat ctgtgcccac
     9121 tcaaaatgtg agtgagtttt atcgaaggtc tgccaaggga taggctttcc tatttttacc
     9181 ctgattgttt ttcctttttt gttaaacagt tctcttggca gcagtgcaag ttcaatattt
     9241 gtctttattc cgaatagttt cctgaacata tagatgaata tgaaaagatt ggaatttcta
     9301 cctctaaagc aaattggcac aacatctctt ttacttgaaa ttgcctttga aataaaactt
     9361 ttttgccagt cacagtcctc aacttttcta tgatattttc tggagacttc tcctgcagga
     9421 aaatgagaaa ttggatcatt tccgttgtaa acctcttcca gtgcctgaag atattctctc
     9481 gaggaaccct caaatacgtt aacagctgct atcaacgggc ggagttgagg aatatacata
     9541 aatgcctcat tggcaattgc tttgacggtg ccatactttt gtgtaattat gtatgtaagt
     9601 ataagcccat caactatgcc aaaaggatga tttgcaacaa aaaagcaacg accgctttca
     9661 ggcaggtttt ctgttccggt aatttcaatt ttcagattga gctcatcaat tatttctgga
     9721 agaaaatctt tgccaataaa gtccttatac ttgttcatta tgtagttcag ctcatcctct
     9781 ttgatgatac cggcaataat atttgttaca aatcgaggta gtgattgcaa aaatgtcgaa
     9841 ttgccttcag aaagtatttt ccttatgtcc acgtacttaa cttcttgctc catgtatatt
     9901 aattttgggt tgtaataagc aattttatgg ccaaaatttt ggataaaata aaaaaaattt
     9961 tcacttttgc tgcaaatgta agagcaatga tgattttcaa caataatttt aaccttaata
    10021 atgacaattc taataaattg ccagggggaa gttgttgata gtgtttaaga tataatttat
    10081 tagcgacctc ccctgcaact caggggaggt tttattttta tagatatata atacatggat
    10141 agaacattaa ttccgaaggg ctataagtcg cctcttgata tttggcagac agagctggca
    10201 atcaaattta ttaaggacac atttcaggtt gaattgtctt ctgagcttaa actaagaaga
    10261 ataacagccc cgctgttctt acaggccggt acgggtctca atgacaattt aaatggagtt
    10321 gagacacctg tcagttttaa aactgaaggt gggaatggaa atatggctga aatagtacag
    10381 tctcttgcta aatggaaacg ttttgcctta tggcgtcata attttaaacc aggcataggt
    10441 gtgtacacag acatgaatgc aatcagacct gctgagatta cagacaatat tcactcaatt
    10501 tatgttgatc agtgggattg ggaaaaggta atcaaaccat cagacaggaa tgtaaaaact
    10561 ttgtataatt gtgtaagaca catttactca gccattaaac gtactcaatt tcttttgact
    10621 gagagattca cccagctgga tccattcttg ccggatgata tatcattttt tcatgcccag
    10681 gatttacttg aaatgtatcc tgacttggag ccaaaagaga gggagaatga agcagcaagg
    10741 aaatatggag caatttttat tagaggaatt ggagg
//
LOCUS       DAOTQL010000049        10679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5582_length_10679_cov_5.289345, whole genome shotgun sequence.
ACCESSION   DAOTQL010000049 DAOTQL010000000
VERSION     DAOTQL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5582_length_10679_cov_5.289345"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /gene="rocD"
                     /locus_tag="P5293_04225"
     CDS             complement(<1..772)
                     /gene="rocD"
                     /locus_tag="P5293_04225"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HRR49143.1"
                     /translation="MELSNKAKHFIECEEKYGAHNYHPLEVVLSKGKGPYVWDVDGKK
                     YFDFLSAYSAVNQGHCHPKIVNALKEQADTLCLTSRAFYNDCLGPYEEYMHNYFGYDK
                     LLPMNSGAEAVETALKLARRWGYVRKGIPEDKAIIICCEGNFHGRTITIVSLSTDPDA
                     YDKYGPFTPGFIKIPYNDTSALASVLEKYADETAGFLVEPIQGEAGVRVPDEGYLKSC
                     YDLCKKYNVLFIADEVQTGIARTGKMLCCDHENVRPDIV"
     gene            912..1310
                     /gene="rpsL"
                     /locus_tag="P5293_04230"
     CDS             912..1310
                     /gene="rpsL"
                     /locus_tag="P5293_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRR49144.1"
                     /translation="MPTIQQLVRNGRKVIVEKSKSRALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHILRGAL
                     DTAGVDGRKQGRSLYGTKRPKKSSAAPAKK"
     gene            1339..1815
                     /gene="rpsG"
                     /locus_tag="P5293_04235"
     CDS             1339..1815
                     /gene="rpsG"
                     /locus_tag="P5293_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRR49145.1"
                     /translation="MRKTKPKKRILLPDPKYHDTLVTQFVNNLMLQGKKSIAYGIFYD
                     AMDIIGEKMKDSDKAPLEIWKKALENITPQVEVKSRRVGGANFQVPTEVRPERKISLS
                     IKNMILYARKRSGHSMAEKLAAEIMAAYNNEGGAFKRKEDMHKMAEANKAFAHFRF"
     gene            1833..3956
                     /gene="fusA"
                     /locus_tag="P5293_04240"
     CDS             1833..3956
                     /gene="fusA"
                     /locus_tag="P5293_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRR49146.1"
                     /translation="MARDLKYTRNIGIMAHIDAGKTTTSERILYYTGKTHKIGEVHEG
                     AATMDWMVQEQERGITITSAATTAFWNYKNEHYQINLIDTPGHVDFTVEVERSLRVLD
                     GAVATFCAVGRVQPQSETVWRQADKYHVPKLAYVNKMDRVGADFLAVVREIKEKLGAI
                     AIPISLPIGSEDKFEGIVDLISKKAFFYNQENKDLINYSVGDIPQNMLSEVEEWRSKM
                     IESIAEYNDSILEKFFEDPDSITEEEIISAIRKATINMAIVPVCCGSSFKNKGIQYLL
                     DSIIRFLPSPLDIGDVHGINPVTEKEEVRKANPKDPFCGLVFKIATDPFVGRLAFLRA
                     YSGRLDSGSAVLNTRSDKKERIARLYQMHSNKQNPIDFVEAGDICAAVGFKDVRTGDT
                     ICDENHPIVLESMTFPDPVIGLAIEPKTQKDLDKLGIALGKLSEEDPTFTVKTDQETG
                     QTIISGMGELHLDIIVDRLKREFGVEINQGAPQVNYKEAVKSTIQHREVFKKQTGGRG
                     KFADIIFEIGPADEGVTGLQFIDEVKGGNIPREFIPSVEKGFKAAMANGVLAGYEVTS
                     LKVVLKDGSFHPVDSDALSFEICARQAFRKACPKANPVIQEPIMSVEVVTPEDYVGDV
                     IGDLNKRRGQITNMDSKGNAKIVKAKVPLAELFGYVTVLRTLTSGRATSTMEFSHYTE
                     LPPNLAKEVIERAGGKKFNENNDDF"
     gene            3984..4289
                     /gene="rpsJ"
                     /locus_tag="P5293_04245"
     CDS             3984..4289
                     /gene="rpsJ"
                     /locus_tag="P5293_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRR49147.1"
                     /translation="MSQKIRIKLKSYDHMLVDKSADKIVKTVKATGAVVSGPIPLPTD
                     KKIFTVNRATFVNKKSREQFELGSYRRLLDIYSSTPKTVDALMKLELPSGVEVEIKV"
     gene            4336..4410
                     /locus_tag="P5293_04250"
     tRNA            4336..4410
                     /locus_tag="P5293_04250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4371..4373,aa:Ile,seq:cat)
     gene            complement(4471..5733)
                     /locus_tag="P5293_04255"
     CDS             complement(4471..5733)
                     /locus_tag="P5293_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49148.1"
                     /translation="MESTMKEEILKHMDDAKDLERLYRSNGSLFKREFMAIYSEIGES
                     PIAKSWYERLTYESELTSKGNKKELFVVVLLALVAGLIVKLNSILGIDEEFYYTRNIG
                     FVVFPVLFAYFAWINRLSLKKNIISGAFFVLLAVFINLLPNSQSDTVILACIHMPLLL
                     WALLGFAFVADADDRNTRRLAYLKYNGDLVVMSTLLIIAGMILSLVTLGLFELLGMNI
                     EDFYMGNIGRVGFAITPVVATYLIQKNPQLVGKISPVIARIFSPLVLIMLVVYLVAIV
                     YSGKDPYNDRTFLLIFNALLIGVLAVIYFSVSESSGNPVNKLRIWILFLLSAVTIIVN
                     GIALSAILFRISEWGLTPNRTAVLGANTLVLVNLILVAVQLIKVIAGKSNIGRIGIVI
                     SRYLPVYIIWAAIVTFVFPLLSQLGIGF"
     gene            complement(5744..6046)
                     /locus_tag="P5293_04260"
     CDS             complement(5744..6046)
                     /locus_tag="P5293_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRR49149.1"
                     /translation="MISTAGLHKAFESRVRLGIMSVLAVNDKLDFNSLKEYLDVTDGN
                     LATHIKALEREEFIGVEKSFVGKKPNTRYFMTQKGRKAFNEHLRALEKFIEDNKKQ"
     gene            complement(6043..6642)
                     /locus_tag="P5293_04265"
     CDS             complement(6043..6642)
                     /locus_tag="P5293_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49150.1"
                     /translation="MEKIKDYVKEITEIRSMMERSSKFMLLSGWAGIIAGLIAIAGTF
                     VAFKFLHFSSSTIFCEAKISVLWLALAVLTTALTVAVYCSYNKAVKRGEKLWNPTTRR
                     MLLSLSVPLITGVLIIIVLIINGLNGLIAPFTLIFYGLALFGGGYHTLPEVKVLGLVE
                     IFLGIVALFIIKYALIFWATGFGLAHIIYGIYIHLKYER"
     gene            6782..8053
                     /locus_tag="P5293_04270"
     CDS             6782..8053
                     /locus_tag="P5293_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HRR49151.1"
                     /translation="MKRFYLILSLTILLIPQLLLSQTTKEEFLSNIKYASGIYQPYNY
                     VKTTKTPAPKDYTPFYISHYGRHGSRWIQTQETYEYPRSILAKAHNDSKLTQLGKSVY
                     QRINTAAQDAWKRYGDLSQLGAKEHKAIAERMFDSFPEVFSTRNGKKCTIYSRSTVVP
                     RCILSMAANNEKLKEFNPQIETIREASDRNYYLNNRYTQVYRDSISKIVNNFLLKHVN
                     FDTFLNSLFNDMTYAHNVVDSPVTFMRNIHLIATDIPCVDNLDFTLFDIFTKDELFYL
                     WQANNMSTYYACGPSNVNGKVAKDSSKLLLKDILDCAEKAIKGHGVSADLRFGHDSYI
                     IPLLALMDIEGMNVAIDDPEKISEVWSDFKVSPMGANLQIIFYRSSKNPEILVKILHC
                     EKEVKIPLKSDIAPYYRWSDFKKYYTTKLAN"
     gene            8107..9339
                     /locus_tag="P5293_04275"
     CDS             8107..9339
                     /locus_tag="P5293_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRR49152.1"
                     /translation="MGKKVKLALHWQILIGIGLGVLLGIFGHEYTIYTKWLGDLFLNG
                     LKMVVIPLVFSALIMGVSSIGETKDLGRIAGKTLLYYLFTTLIACILGLSLVNAFRPG
                     FGMELNLKEEVTSIAATQVSIIDQIVNIVPTNIVKAMADGNMLPVIFFAILFGIFITL
                     SSQKSRVIMTDFFNSVYEVMIKMTFLVIRFAPLGLFGVVSSIIGTQAGDSKALGEMFG
                     SLGIYAAIIAGGCLIQGLGILPLIFVAFTKKNPFKFMSQMGTGLLTTFCTASSAASLP
                     ITIPDIQSKTGVSQKIAAFCLPLGNTINMNGTALFECVTAIFIAQVYGIDLTFGQQIM
                     VVLTSLLAAVGSAGIPLAGLVMLAVVLNAVGLPLEGIGLVLVVQQPLDMIRSAVNVWG
                     DLTGAVIVAKSEGETITI"
     gene            9344..10450
                     /locus_tag="P5293_04280"
     CDS             9344..10450
                     /locus_tag="P5293_04280"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRR49153.1"
                     /translation="MNSKFICPSCGAEYSTEEPIWRCNCGSYLDLFFKPKFEKKLLSN
                     EKTLWRYSQVLPVDKRFAVTFDEGYTPLISSDFWGINVLCKLDFMFPTGSFKDRGAAL
                     LIAKCKELGITEIVEDSSGNSACSISAYCARAGIKANIYMPAGNSTGKAVQAKAYGST
                     IHLVQGNREDCANAALEGAKKSYYAAHAWNPYFLQGTKTVIYETVEQLGWDVPDYIFV
                     PVGSGTLLLGIYLGLRELISLSIIDKMPKLIAVQSEKCSPLKDYVEGKKGCNSISDKS
                     IAEGIAIHKPVRLTQIVNAVTESKGDFVTVDDSTIIVALKESAHQGIFIEPTSAAAIA
                     ALKQYAGKISPPSKVLVILTGSGLKTTATIEKLL"
     gene            complement(10470..>10679)
                     /locus_tag="P5293_04285"
     CDS             complement(10470..>10679)
                     /locus_tag="P5293_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49154.1"
                     /translation="GVARRNAAINLTCRLIDTSDGSQVALVMIMNSSANSFSGTDFDS
                     GYRIQECYAKAGRELAKFIIKECKL"
BASE COUNT         3490 a         2110 c         1999 g         3080 t
ORIGIN      
        1 taacgatatc cggcctcaca ttctcatggt cacaacataa catttttccc gtacgcgcta
       61 tgcctgtttg tacttcatct gcaataaaga gaacattata tttcttacat aaatcataac
      121 agcttttcag atatccctca tccggcaccc tgactccagc ctctccctga ataggctcaa
      181 caagaaaacc agctgtttca tcagcatatt tttcaagaac agatgcaagt gctgaagtat
      241 cattgtaagg tattttaata aatccaggag taaaaggacc gtatttatca tatgcgtcag
      301 ggtctgtgga aagagagaca atagtaatag tccttccgtg aaaatttccc tcacaacaga
      361 taataatagc tttatcctca ggtatcccct ttctgacata tccccatctt ctggcaagtt
      421 tcagcgcagt ttcaacagcc tctgcaccag aattcatggg aagtaactta tcatatccaa
      481 aatagttatg catatactct tcatatggac caagacagtc attataaaat gctcttgatg
      541 taagacatag agtgtctgct tgttctttaa gtgcatttac aatttttgga tgacagtgac
      601 cttgattaac agcagaatag gcagataaaa aatcaaaata cttttttccg tcaacatccc
      661 atacatatgg acctttacct ttcgaaagta ctacctcaag cggatgataa ttatgagctc
      721 catatttttc ctcacattca atgaaatgtt ttgctttatt tgataattcc ataattttga
      781 attttctcaa agatagtcct tttttcctca aaaaatgtca atcagatgtt tgttattaga
      841 attaataatc ttacctttgc aacccaattt tatttggcga aaatatttaa agcaaagaac
      901 aacaacaaat tatgcctaca attcaacagt tagtccgcaa tggacgcaaa gtaatcgtag
      961 aaaagagcaa atctcgcgct ttggattcgt gcccacagag acgcggagta tgtgtacgag
     1021 tttacacaac aactccaaag aaacctaatt ctgcaatgcg taaggtagct cgtgtacgcc
     1081 taaccaacca gaaggaagtt aacgcttaca ttccgggaga aggacacaat ctgcaggaac
     1141 actcaattgt gcttgttcgt ggtggtcgtg ttaaggatct tcctggtgtt cgttatcata
     1201 tcctgcgtgg tgcccttgat acagcaggcg ttgatggacg caagcaaggc cgttcccttt
     1261 atgggacaaa gagacctaag aaaagttcgg ctgctcctgc aaaaaagtaa ttttacttaa
     1321 ataacaaatt tataaccaat gagaaaaacg aagcctaaaa agaggattct acttcctgat
     1381 ccaaagtatc atgatacact ggtaacacag tttgtcaaca accttatgct tcaggggaag
     1441 aagagtatcg cttatggcat cttttacgat gccatggaca tcatcggcga gaagatgaaa
     1501 gattctgaca aggctccttt ggagatctgg aaaaaagctc tcgaaaacat caccccacag
     1561 gttgaggtga agagccgtcg tgtaggtggt gcaaacttcc aggtccctac agaagtgcgt
     1621 cctgaaagaa aaatttcatt atcaattaaa aacatgattc tgtacgcacg taaacgttca
     1681 ggtcattcta tggccgaaaa actcgcagcc gaaataatgg cagcatacaa taacgaaggc
     1741 ggtgcattca agagaaaaga agatatgcac aagatggcag aagcaaataa ggcatttgcg
     1801 cacttccgtt tctaagttcc aggaaataat tgatggcaag agatttaaaa tataccagaa
     1861 atattggcat catggctcat attgatgccg gtaagacaac tacatccgaa cgaatcctct
     1921 actatacagg caaaacacac aaaatcggag aggttcacga aggtgccgct acaatggatt
     1981 ggatggtcca ggagcaggaa agaggtatta caataacctc tgcagcgact actgcttttt
     2041 ggaattataa aaacgaacat tatcagatca atcttatcga tactccgggc catgtcgact
     2101 tcactgttga agttgagagg tctctccgtg tactggatgg agcggtcgcc actttctgtg
     2161 cggttggacg ggtccagcct caatcagaaa ctgtatggcg tcaggcagat aagtatcatg
     2221 tccctaaact ggcctatgtc aacaagatgg atcgtgtggg agctgatttt ctggctgttg
     2281 ttcgcgagat aaaggaaaag ttaggggcta ttgctatacc gatttcactt ccaattggtt
     2341 ccgaagacaa atttgaagga atagtggatt tgatatccaa aaaagcattt ttctataatc
     2401 aagaaaataa ggatctgata aactattcag tgggtgacat tcctcaaaat atgctatccg
     2461 aagttgaaga atggagaagt aaaatgattg agtcaatagc tgaatataat gattcaatac
     2521 ttgagaaatt ttttgaagat cctgattcca taaccgaaga agaaataatc tctgcaatca
     2581 ggaaggctac cataaatatg gctattgttc ctgtatgctg cggctcttca ttcaagaaca
     2641 aaggcattca atatctcctc gattctatta ttcgcttcct gccatctcca ctcgacattg
     2701 gagacgttca tggaataaat cctgttacag aaaaggaaga agttcgcaag gcaaatccaa
     2761 aagatccatt ctgtggtctt gtattcaaaa ttgctacaga tccttttgtc ggccggcttg
     2821 cattccttag agcatattct ggaaggctgg attcaggttc tgcagtgttg aatacacgtt
     2881 cagacaagaa agaaagaatt gcaagactct accagatgca ttccaataaa caaaacccga
     2941 ttgattttgt tgaagccggg gatatctgcg ctgcagttgg attcaaggac gtacgaacag
     3001 gagatacaat ttgtgatgaa aaccatccta ttgttctaga atcaatgaca tttcccgatc
     3061 ctgttatcgg acttgcgatt gaacctaaaa cccagaaaga tctcgacaaa ctcggaatag
     3121 cactcggaaa actttcagag gaagatccta cattcacagt taagacagat caagagacag
     3181 gtcaaacgat aataagcgga atgggtgaat tgcacctcga tattattgtt gaccgtctta
     3241 aaagagagtt tggtgtagaa atcaatcagg gtgcacctca ggttaattac aaagaggcag
     3301 taaaatctac cattcaacac cgtgaagtat tcaagaagca aacaggtggc cgcggtaaat
     3361 ttgcagacat catttttgaa ataggccctg ctgacgaagg agttacagga cttcaattca
     3421 tcgacgaggt taaaggcggt aacatcccga gagagtttat tccatctgtc gaaaaaggtt
     3481 tcaaagcggc aatggcaaac ggagttcttg caggttatga agtaacttca ctcaaggttg
     3541 tcctcaagga tggttcattc caccctgtcg attcagacgc tctatcgttt gagatatgtg
     3601 caagacaagc gttccgtaag gcatgtccta aggccaatcc ggtaatccag gaaccaatta
     3661 tgtctgttga agtagttact cctgaagatt acgtgggaga tgttatcgga gacctcaaca
     3721 aacgaagggg acagattaca aacatggact ctaagggtaa tgccaaaatt gtcaaagcaa
     3781 aagttcctct tgcagagctg tttggatatg ttacagttct tagaacactt acttccggac
     3841 gtgccacaag taccatggaa ttctctcatt atacagaact ccctccaaat cttgctaaag
     3901 aggtaataga aagagccggt ggcaagaaat ttaatgagaa taacgacgat ttttagtaaa
     3961 ataattggtt aataataaat aaaatgagcc aaaaaataag gataaagctt aaatcttatg
     4021 atcatatgct ggttgataaa tcggcagaca agattgtaaa aaccgtaaaa gcaacagggg
     4081 ctgtagtcag tggtcctatt ccactcccta cagacaagaa gatttttaca gttaaccgtg
     4141 ccacattcgt taacaagaaa tcccgcgaac aatttgaatt aggttcatac cggcgccttc
     4201 ttgacatcta cagttccact cccaaaacag tggatgcatt gatgaagctc gaacttccaa
     4261 gcggagttga ggttgaaatc aaagtctgag tatacattgc ggcaaattaa taattatatt
     4321 tgccattata aattcgggcc catagctcag ttggtcagta gcacctgact cataatcagg
     4381 gggtcgttgg ttcaagccca actgggccca cttttaaaaa gcacttgcaa actctttctg
     4441 taagtgcttt cttattttat tcaatataat ttaaaaaccg attccgagtt gtgacaaaag
     4501 tggaaacaca aatgttacga ttgcggccca tatgatgtat actgggagat agcgcgaaat
     4561 aacaatccct atcctaccaa tattactttt acctgcaatt accttaatta gttgcacggc
     4621 aaccagtatc aaatttacaa gaacaagagt atttgcaccc aaaactgcgg ttctgttagg
     4681 agtcaagccc cattctgata ttctgaaaag tatagcagag agtgctatac cattgacaat
     4741 aatagtcact gcagacagca aaaataaaat ccatatcctt aatttgttga caggatttcc
     4801 agaggactct gacactgaaa aatatatgac agccaaaacc cctattaaca aagcattgaa
     4861 aataagaaga aatgttctgt cgttataagg atctttccct gaataaacaa ttgctaccag
     4921 atatactact aacatgatca gcacaagagg gctgaatatc cttgcaatca caggagagat
     4981 cttacccact aactgtggat ttttttgtat caaataggtt gcgacaacag gtgttatggc
     5041 aaatcctact cttcctatat tgcccatata aaagtcttct atgttcattc cgagcaattc
     5101 aaaaagtcct aatgtcacaa gactgagaat cattccggct ataattagta acgtggacat
     5161 aaccactaaa tccccgttat atttcaaata ggcaagcctt cgtgtattcc tgtcatctgc
     5221 atctgcgaca aaagcaaaac caagtaaagc ccataacaat aacggcatat gaatgcatgc
     5281 aagaattaca gtatctgact ggctgttagg taatagatta ataaacactg ctaacagcac
     5341 aaaaaatgct cctgaaatga tatttttttt cagagacaat ctgtttatcc atgcaaaata
     5401 agcaaacaat acaggaaaca ccacaaagcc gatatttctt gtgtaataaa actcctcatc
     5461 aatacccaaa atggaattca attttactat cagacctgca actaaagcca gcaacacgac
     5521 aacaaacaat tcctttttat tacccttgct tgttaactca ctttcatatg tcagcctttc
     5581 gtaccaagac tttgcgatag gactttctcc tatctctgaa tagatcgcca taaattctct
     5641 tttaaagaga gaaccattac ttctgtaaag tctttcaaga tcctttgcat catccatatg
     5701 tttaagaatc tcttctttca ttgttgattc cataaattta aaattattgt tttttattat
     5761 cctcaataaa tttttcgaga gcacgcaaat gttcattaaa tgccttcctg cccttttgtg
     5821 tcataaaata acgtgtattt ggcttcttgc caacaaacga cttttcaacg ccaataaact
     5881 cttctctttc gagagcttta atatgagtcg caaggttacc atcggtaaca tcgagatact
     5941 ctttgagaga gttgaaatca agcttgtcat tgactgccaa aacagacata attccaagcc
     6001 tcacccgact ttcaaaagcc ttatgtaaac ctgcagtaga aatcaccttt cgtactttaa
     6061 atgaatatat atcccgtaaa taatatgtgc caagccaaaa cctgttgccc aaaaaattag
     6121 agcatatttt attataaata gtgcaacaat tcccaggaat atctccacca aaccaagaac
     6181 ttttacctcc ggcaatgtat gatatcctcc tccaaaaagt gcaagaccat aaaatatcaa
     6241 agtaaaaggt gcaattaacc cgtttaatcc attaataatc aaaacgatga taatcaagac
     6301 acctgtaatc agtggaactg aaagactcaa cagcattcgc ctcgtagtag gattccataa
     6361 tttttcacct cttttaacag ctttgttgta ggaacagtaa acagcaactg taagggcagt
     6421 tgttaaaact gccaaagcca accataatac gcttattttg gcttcacaaa atattgttga
     6481 ggaagaaaag tgcaaaaatt taaaagccac aaacgtacct gcaatagcaa ttaaaccggc
     6541 aataatccct gcccatccgg acaaaagcat aaactttgag gatcgctcca tcatcgagcg
     6601 tatctctgtt atttccttta cataatcttt tatcttttcc attaaaagta ctttttatta
     6661 caaagtaaaa tttattattt gaaagttcca aatttgtcaa agaatttttt ttatgtttac
     6721 ttaaaataag aaataaatta tctttatagg caaataataa taaaccaact ctcagtatat
     6781 catgaagcga ttctatctaa ttcttagtct tacaattctg cttattccac aattattatt
     6841 gtctcaaacc acaaaagaag agtttctaag taatatcaag tatgcaagtg gcatttacca
     6901 accttacaac tatgttaaaa caaccaagac acctgctcca aaagattata ctccgtttta
     6961 cataagtcac tatggaagac atggttcaag atggattcaa actcaagaaa catatgaata
     7021 ccctcgttcc atacttgcca aagcacacaa tgatagcaaa ttgacacagc ttggaaaaag
     7081 tgtataccaa agaattaata cagccgcaca agatgcttgg aaaagatatg gagatctttc
     7141 acaacttggt gctaaagaac acaaagcaat agcagagagg atgtttgact ctttccctga
     7201 agttttttca acacgaaatg gtaaaaaatg cacaatatac agtcgctcga cagttgtacc
     7261 gcgatgcatt ctgagcatgg ctgccaataa cgagaagctt aaggaattca atccacaaat
     7321 tgaaacaata agagaggcat ctgacagaaa ttactatcta aacaaccgtt atactcaagt
     7381 atacagagat tccatttcaa aaattgtcaa taattttctt ctcaaacatg ttaattttga
     7441 cacatttctt aattcattgt ttaatgatat gacctatgct cataatgttg ttgacagtcc
     7501 tgttacattt atgagaaata ttcaccttat agctacagat attccatgcg ttgacaacct
     7561 tgattttacc ctctttgaca ttttcacaaa agacgagctt ttttatctct ggcaggcaaa
     7621 caatatgtct acatactatg catgtggccc ttcaaacgta aatggcaaag tagcaaaaga
     7681 cagttccaaa ctgcttctca aagatatact cgactgtgcc gaaaaagcca taaaagggca
     7741 tggtgtatct gctgatctga gatttggaca cgattcttat attattccgc tacttgctct
     7801 tatggatata gaaggcatga atgtagccat agatgatcct gaaaaaatca gcgaagtatg
     7861 gagtgacttt aaagtaagtc ccatgggagc aaatctgcaa attatatttt acagaagcag
     7921 taagaatccg gaaatcctgg ttaaaatcct tcactgcgaa aaagaagtta agataccttt
     7981 aaaaagtgat attgctcctt attaccgatg gagcgacttc aaaaaatatt ataccaccaa
     8041 acttgccaat taagatctct gtgacccaat ttatctattt aattataaaa acttcaatca
     8101 gactatatgg gcaaaaaagt taaactcgcc ttacattggc agattttgat aggtatcgga
     8161 ctcggcgttc tgctgggtat tttcggacac gaatatacta tctatacaaa atggttgggt
     8221 gatctgtttc ttaatggtct caagatggta gttataccat tggtcttcag tgctcttata
     8281 atgggagttt cgagcattgg ggagactaaa gatcttggaa gaattgcagg caaaaccctt
     8341 ttatattatt tgtttacaac actaattgcg tgtattttag gtctttcact tgttaatgct
     8401 ttcagacctg gtttcggaat ggaacttaac ttaaaggaag aggttacatc aattgcagct
     8461 actcaagtat caataattga tcagattgtc aatattgttc cgaccaatat tgttaaggcc
     8521 atggcagatg ggaatatgct gcctgtaatc ttctttgcaa ttctgtttgg aatattcatt
     8581 acactctctt cccagaagag ccgcgttatt atgaccgatt tttttaacag cgtttacgaa
     8641 gtgatgataa agatgacctt cctggttatc cgctttgctc ctttaggcct tttcggcgtt
     8701 gtatcctcaa ttatcggaac tcaggccggg gactcaaaag ctttgggaga aatgttcgga
     8761 agccttggaa tctatgcagc aataatagcc ggaggttgtt taatacaggg acttggaata
     8821 ttgccactga tatttgttgc atttacgaag aaaaatccat tcaagttcat gagccagatg
     8881 ggtacagggc ttctgacaac attctgtacc gcatcttctg ccgcttcatt accaattaca
     8941 atacctgaca tacaaagtaa aacaggagtt tctcagaaaa ttgctgcttt ttgcctcccc
     9001 ttgggaaata caataaatat gaacggtact gcactttttg aatgcgttac agctattttc
     9061 atagcccagg tctatgggat agatttaaca tttgggcaac aaattatggt agttctgaca
     9121 agtcttcttg cagcagtagg ttctgccgga attcctctgg caggacttgt tatgctcgca
     9181 gtagtattga atgcagtagg tttgccatta gaaggaattg gattagtcct tgttgttcag
     9241 caaccacttg acatgattcg ctcagctgta aatgtatggg gagatttgac aggtgcagtc
     9301 attgttgcaa aaagcgaagg agaaaccata accatttgat attatgaata gtaaatttat
     9361 ttgtccttcc tgtggggctg aatattctac agaagaacct atatggcgtt gtaattgtgg
     9421 tagttattta gatttatttt ttaagccaaa atttgagaaa aagttacttt ccaatgaaaa
     9481 gacattgtgg cgctacagtc aggttttgcc agttgataaa agatttgctg tgacgttcga
     9541 tgaaggttat acacctctga tatcctctga tttctgggga attaatgttt tgtgcaaact
     9601 tgactttatg tttccaacag gttcattcaa agacagaggt gctgccttat tgatagcaaa
     9661 gtgtaaagaa ttgggcataa ccgagattgt ggaggactcg tcaggtaatt cagcctgctc
     9721 catatcggca tattgtgcaa gagccggtat taaagccaac atatatatgc cagctggcaa
     9781 ttctacaggt aaagctgtgc aggcaaaagc ttatggatca actatccatc ttgttcaagg
     9841 gaacagagaa gattgtgcaa atgctgccct cgaaggggcc aaaaagagct attacgctgc
     9901 tcatgcttgg aatccatact tcttacaagg gactaagact gtaatatatg aaactgttga
     9961 acagttaggc tgggatgttc cagattatat atttgttcct gttggcagtg gaaccctgct
    10021 tttagggatt tatttagggc ttagagaact aattagttta tcgattattg ataaaatgcc
    10081 aaaattaata gccgttcaaa gtgaaaaatg ttctccacta aaagattatg ttgaaggtaa
    10141 aaaagggtgt aattcaatca gcgacaaatc aattgcagag ggtatcgcta tacacaagcc
    10201 tgtaagactt acacaaatag taaatgctgt tacagaaagc aaaggcgact ttgtcactgt
    10261 tgacgattct acaattattg ttgctttaaa agaatcagca catcagggta ttttcattga
    10321 accaacatct gcggcggcaa tagctgcact aaaacaatat gctggaaaaa tctctccacc
    10381 cagtaaagtc ttagtaatcc ttacaggaag tggactgaaa actacagcca caattgaaaa
    10441 attgctttaa atgttgatga gacaggatgc tacagtttgc actcctttat aataaacttt
    10501 gccagttccc tgcctgcttt agcataacac tcctgaattc tgtagcctga atcgaaatct
    10561 gtccccgaaa aactattggc agaagaattc attatcataa cgagtgccac ttgagaacca
    10621 tctgaagtat ctatcaggcg gcatgttaga ttgatagctg catttcttct tgcaacacc
//
LOCUS       DAOTQL010000050        10651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5603_length_10651_cov_4.725840, whole genome shotgun sequence.
ACCESSION   DAOTQL010000050 DAOTQL010000000
VERSION     DAOTQL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5603_length_10651_cov_4.725840"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2353
                     /locus_tag="P5293_04290"
     CDS             <1..2353
                     /locus_tag="P5293_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49155.1"
                     /translation="EFSRSGRGRDPEQMIYFKASFLDGDYRNWPKWKLGSGRLFTPDK
                     YSLGYFVHSYIRTSSGNSTYIGDITDYVADHFYNPFCVKKAYKSYTGYTIREHFENIK
                     YSIGEKWKNEDSLKRPLTLYKRISHKGNDYSSYRSVVSISPDTLFAIRSDMDEPARLV
                     MLDSSGNEKRIAYMGNRSSYLVYSNGKMFWTEQFPSLRWELESYSVLREYDVKSGKIQ
                     TVTSKSSYSNPSFSFTGDTVAVSEYLPEGGSSVVLLNTFSFDIIGSFKVPNGAQVLET
                     LFMGNTILYATVITESGLGLYKLDTSSGEWGNEISDQSRYITRLNRTPDGIIFESDLD
                     GTNNLYCYNPQLKFLQKLTNSRFGAFEPNVDERGNVYYSEYDSGGYFIAKADRDSLKW
                     EPALFSKPAKIEIADLLSLEAGYCIDSVHVEQGMRYESKPYRKGANLFNFHSWAPFYY
                     NVDKLMNKSFDSMYEAIAPGFTIYSQNLLGSAVTMFGYSYVSGFHAGHMSFNYSGLFP
                     VIEVSMDYNTRWRREIRMIQDINRKKYQLFYDVNGSPMFTSDLLLYLPLNFSKDGWNR
                     GLLPSLLWRFSNDSYYSIENNRYNYYQYISAGIQYYSVLKMAARDIFPKWGWGMVFKL
                     NRVPFSNENYGSLFYSRVYAYMPGLLRNHGLKIGFSFQYQDFSGKNYLLSNIADSPRG
                     YDQLYGKRYYSFSIDYALPLYLGDIEIRPLLYLKRLQVIPFTDYAYNVGSRENKNMLS
                     VGSDLLLDFNILNISIGLSAGLRFAHTAENNNVFQFLFNIPIY"
     gene            complement(2376..4175)
                     /gene="uvrC"
                     /locus_tag="P5293_04295"
     CDS             complement(2376..4175)
                     /gene="uvrC"
                     /locus_tag="P5293_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRR49156.1"
                     /translation="MIQRPDIRETLKILPAQPGVYRFVNKEGIIIYIGKAKNLRNRIS
                     QYFQSENTLSPKTKAMVSKIDHIEYTVVNSEEEALLLENNLIKKYQPRYNILLKDSKT
                     YPWICIKNEPFPRVFVTRKYSKDGSLYFGPYSSAMYAHSLIDLINTLYQIRNCKLSLT
                     ESNIKTKNFRPCLNYHIGKCKAPCAGYISDHRYMQQIDGVKSILKGETSRLKKEFKES
                     MLKAAQEMRFEEAQNLKEKLDLLEKHYSKSTIVNPLITNIDVFSLAQEDNIAFGNFLR
                     VVNGSIIQSLNLEFRLQIEENMSDVISTFMAEIRSKFGELSSEILVSVMPDENFSGKQ
                     VHIPHRGDKLKLLELSIKNANQFKAERIKQEETLRPEDHFNRLLSTLQKDLNMDQLPI
                     HIECFDNSNIQGQYAVAACVVFKDGMPSKKDYRHFNIKSVVGANDFATMKEVVNRRYS
                     RLLAENAPLPQLIVVDGGRGQLNYAYEALRELDLHNKIKIIGIAKRLEELIIPGDPDP
                     LFLDKNSSSLKLIMHLRDEAHRFGIKHHRNQRSKGQINSELRQIKGIGEKKEELLLGK
                     FKSLKAIKNATFEELTETVGGKTAKIIYDYYHK"
     gene            4220..>5663
                     /locus_tag="P5293_04300"
     CDS             4220..>5663
                     /locus_tag="P5293_04300"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRR49157.1"
                     /translation="MNRVVTKSDVKKAIKLKGFPGNIAASFLMWILGLNTLNKKYSKI
                     CQFEGRDFTTAVMREFGFGYELDDRQLKNIPQEGPFILVSNFPYGSIEGIILLHIVSA
                     VRPEIKFLGNFMLSIIPNLRDYIFPINPFVDHPELHNSFSGLRQASETLANGGCLGLF
                     PAGEVSTYYGGIFIQDKEWQISIIKLIKNAGVPVIPVYCHGTNSRFFHWAGRIHPLLR
                     TLRLPKELLNKRGKIVSVRIGKSVSSYEISEYKDLKSLGAYLKARTYALEANIKTAQK
                     IEARALTPIALPNNRKHLQKEINTLPQNCLLFSTGNYVCYLADYKLIPMMMHELGRRR
                     EESFRAVGEGTGKPLDLDEYDEYYKHLILWDKYRQRLVGAYRLGFGKNIIETRGVKGL
                     YSNSLFGYEKPFQDVLKQSIELGRSFVTLDYQKDNLSLMLLIKGLLYSVLSHPEVKYL
                     IGPVSISSWYPKFYRSVMIYYIQNKHSINDF"
     assembly_gap    5664..5763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5794..6534)
                     /locus_tag="P5293_04305"
     CDS             complement(5794..6534)
                     /locus_tag="P5293_04305"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRR49158.1"
                     /translation="MKAKRILISQPAPTNGSSPYTDLITKYKLSIDFVPFFKVEPLQA
                     KEFRLQKINILDHTAVVFTAKTAIDAFFKICEEMRITVPETMKYFCNSESIALYLQKY
                     IVYRKRKIFFGNGTNASIIDAIGTKHKNENFLVCVTDSSKADIHKIFVKAKLKHTSGV
                     FVKTVFSDLSNLELNNYDMLVFYSPSDIRSLKQNFPDFKPNGIQFATFGPATLKALKG
                     AKLTSTVIAPTPEAPSIAKALSIYFEQK"
     gene            complement(6538..7314)
                     /locus_tag="P5293_04310"
     CDS             complement(6538..7314)
                     /locus_tag="P5293_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRR49159.1"
                     /translation="MEQTCAYPYDYSWIPDSIQTLPVDSLWGKDTFYITGSNYTTPRR
                     EKSENDSIMSGLFLAFFIVLIIFSKEIINVYPSIIKSFFKLKNHEKIEERLAISNQRN
                     IVVGIFFYYYPLMLSLTLREQIVTKYNITPPLFFLILLGTVICLWLVRKGALALLSWL
                     LNENYAFNFIEKISYNYFIISVFFTFPISLASFFWPELDNKLFINLLIYCVLFVYFIY
                     LVKTWQVIRSRQFSLFFYILYLCTLEFLPIALTVDLILTI"
     gene            complement(7314..7664)
                     /locus_tag="P5293_04315"
     CDS             complement(7314..7664)
                     /locus_tag="P5293_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRR49160.1"
                     /translation="MSQNDWKSRLGIVFSTDPDFNYTKDNNKDDAIHTLKPEKQYLTV
                     AIDRKQRAGKQVTLIKGFQGSKEDLESLAKILKNKCGTGGSAKEGEIIIQGDFRDKLI
                     NILQGMNYKAKRGN"
     gene            7748..>10651
                     /locus_tag="P5293_04320"
     CDS             7748..>10651
                     /locus_tag="P5293_04320"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRR49161.1"
                     /translation="MEQKKFKLINNLLGLLVFIIAAFTYLSTIEPTASFWDCGEFIAS
                     SYKLEIGHPPGNPVFQLIARFFTLFADNQHAAMMVNAMSAICSALTIMFLFWTITHLV
                     RRIVEKHKETVTAATAISVFGAGLVGSLAYAFSDTFWFSAVEGEVYAMSSLFTAVVFW
                     AMLKWEECADEPHANRWIVLIALLMGLSIGVHLLNLLTIPALVFIYYYKRYNVTNKGL
                     VWVFVLSCVILAFILYGIIPFLPKIAAWFDLFFVNVLGFGINTGAAIFLLLLLIASLW
                     SVWYTYKKGKVLANTILLCFTMIVIGYSTFAVVIIRSSANTPTNENQPDNAFSLVRYL
                     AREQYGSNPLIYGETFASPYDIKQESYYAKVDNKYKKVEGPVQPVYDPASKMVFPRMW
                     SSASDDHVKFYKMYTEGRGKSIVGSDEKIPLMKDNLAFFFDYQLNWMYLRYFMWNFAG
                     RQNDLHGSTPGDLLRGNWESGITFFDRARLGDQSEGPAYIVKSKAKNHYYMIPLFLGI
                     LGFFYQLAKDKRNTWIVTLLFILTGVAIVVYLNQTPYQPRERDYAYAGSFYAFAIWIG
                     LSVTALYDAFRKRIPALVTASAVTLVTLCVPVLMACENWDDHDRSNRYSARDLAYNYL
                     NSCDKQAILVTHGDNDTFPLWYIQEVEGVRTDVRIMNTSLLGTDWYIDQMKRKTYESE
                     PVKLSLPRSEYLYGTNDMIHIVDRLNRPVPIKAVIDLIANPEAKLQAENGKRYSFVAS
                     KQLLLPVNVKNAIACGIVSEADSAQILDTLLLKIPEKKNYLTKCEMIILDMLANYDWS
                     RPIYLVSMGGDLELGLRDYLEYNGFAYKFVPIKSSTSLGSPGRVVPEVMYDKCMNVYR
                     WGNLNDPKVNVDYQNLLTFNALMCGRNIYSTTARSLLECGKTKEAVEVLDKMQEYFIP
                     SQFPLNCSLLPSLNEISVMEAIDIYLQAGERDKGLALVKAFTDETYQALNLFTK"
BASE COUNT         3181 a         1694 c         2069 g         3607 t
ORIGIN      
        1 agagttttca agatcaggaa ggggtagaga tcctgaacag atgatttatt tcaaggcatc
       61 atttcttgat ggagattata gaaattggcc taaatggaaa ctgggctcgg ggagattgtt
      121 tactcctgat aagtatagct tgggttattt tgttcattca tacatacgta catcttctgg
      181 aaacagcacg tatataggag atataacaga ctatgtggca gaccatttct acaatccttt
      241 ctgtgtaaaa aaggcttaca agagttatac ggggtataca attagagaac acttcgaaaa
      301 catcaagtat tcaattggag aaaagtggaa gaatgaagat tctctcaaac ggcctttaac
      361 tttatataaa cgaatttctc acaaaggtaa tgattactca tcttatagat ctgttgtaag
      421 tatctcgcct gatacactct ttgcaataag aagtgatatg gacgaacctg caagacttgt
      481 aatgttagat tcttcaggaa atgagaagag aatagcctat atgggaaaca ggagcagtta
      541 tcttgtatat agtaatggga aaatgttttg gactgaacag ttcccatctt tgagatggga
      601 acttgaaagt tattcggttt tgagagagta tgatgtaaaa agtggtaaaa ttcaaaccgt
      661 gaccagtaaa agcagttatt caaacccttc tttttctttt actggagaca ctgttgctgt
      721 aagcgaatat ttgcccgaag gagggtcaag tgtcgtttta cttaatacct tttcttttga
      781 tattattggt tcgtttaaag tgccaaatgg tgctcaggtt ttggaaacac tttttatggg
      841 gaatactatc ttatatgcca ctgttattac tgagtcggga ttagggttat ataagcttga
      901 tacatcctcc ggagaatggg gaaatgaaat ttcggaccag agcagatata ttacgaggtt
      961 gaacagaacg ccggatggaa taatattcga gagcgatctg gacgggacta acaatttata
     1021 ttgctacaat cctcagctca aatttcttca gaaactcact aattcaagat ttggagcatt
     1081 tgaaccaaat gtcgatgaaa gaggtaatgt ttactattca gaatatgaca gtggaggata
     1141 ttttattgca aaagctgaca gggattcact taaatgggaa ccggctcttt tttctaagcc
     1201 cgcaaagatt gaaattgctg atttactatc acttgaagcg ggttattgca ttgatagtgt
     1261 gcatgtagaa caaggaatgc gatatgaatc aaagccttac agaaaagggg ctaatttatt
     1321 caatttccac agttgggcac cattttacta caatgtagat aaattgatga acaaatcttt
     1381 tgatagtatg tatgaagcta tagccccggg ttttacaatt tattctcaaa atttgctcgg
     1441 gagtgctgtt acaatgtttg gatattccta tgttagcggg tttcatgcgg gtcatatgag
     1501 tttcaattat tcgggactgt tccctgttat tgaagtatca atggattaca acacaagatg
     1561 gaggagggag atccgaatga ttcaggatat taatagaaaa aaatatcagt tattttatga
     1621 tgtgaatggg tcaccaatgt ttacctcaga tcttcttttg tatttgcctt taaatttttc
     1681 gaaagacgga tggaacagag gtttgctgcc aagtttactt tggcgtttca gtaacgattc
     1741 ttattattct attgaaaata accgatataa ttattatcaa tatatttcag caggtattca
     1801 atattacagc gtactgaaaa tggctgcaag agatattttt cctaaatggg gatggggaat
     1861 ggtgtttaag ttaaacagag ttccattttc aaatgaaaat tatggatcac tgttctattc
     1921 aagagtgtat gcgtatatgc ctggattact tagaaatcat ggccttaaaa taggtttttc
     1981 gtttcaatat caagattttt caggtaaaaa ctatctctta tccaatattg cagactcacc
     2041 gagggggtat gaccaacttt atggtaagag atattattct ttttctattg attatgcctt
     2101 gcctctctat ctgggagata tagaaatacg tcctctatta tatcttaaaa ggctgcaagt
     2161 tattcctttt actgattatg catacaatgt gggttcaaga gaaaataaaa acatgctttc
     2221 agttgggagt gatctgcttc ttgactttaa tatattgaat atatctattg gactttcagc
     2281 aggattaaga tttgcacata cagctgagaa taataacgtg tttcagtttt tgtttaatat
     2341 tccaatatat tgaatattca ttatataatt tgaatctact tgtgataata gtcataaatt
     2401 attttggctg tttttccccc aacagtttca gttaattctt caaacgtcgc atttttaata
     2461 gcttttaaac ttttaaattt accaagcaag agttcctctt ttttttctcc aattcctttt
     2521 atttgtctta actcagagtt aatctgtcct ttgctgcgtt gattacggtg atgtttgatg
     2581 ccaaagcggt gggcttcatc tcttaaatgc ataatcaatt taagagagga agaattttta
     2641 tcgagaaaca gtggatcagg atcgccagga attatcagtt cttcgagcct tttagctatt
     2701 cctatgattt ttattttatt atgtaagtcg agttcgcgaa gtgcctcata tgcataattt
     2761 aattgtcctc ttcctccatc aactacaatg agctgaggaa gcggggcatt ttctgcaagt
     2821 agtcttgaat aacgacggtt tacaacttct ttcatagttg caaaatcatt tgcgccaact
     2881 acactcttta tgttaaaatg tctatagtct ttttttgacg gcatgccgtc tttaaaaact
     2941 acacaggcag caactgcata ctgaccctga atgtttgaat tatcaaaaca ctcaatatgg
     3001 atggggagtt gatccatgtt taaatctttt tgcaatgttg aaagtaatct gttgaagtga
     3061 tcttctggcc ttaatgtttc ttcttgtttt attctctctg ccttgaactg gtttgcattc
     3121 ttaatactga gttcaagtag tttgagctta tcacctctat gaggtatgtg aacttgtttg
     3181 ccggagaaat tttcatcggg cattactgat acaagtatct cagaggagag ctctccaaat
     3241 tttgaacgaa tttcagccat aaaggtgctt ataacatcgg acatattctc ttcaatttga
     3301 agtctgaact caaggttaag agattgaatt atagaaccgt ttacaactct aagaaaattt
     3361 ccaaaagcaa tattatcttc ttgtgcaagt gaaaatacgt caatatttgt tattaaagga
     3421 tttacaatag tagatttgct gtaatgcttt tctaataaat caagtttttc cttaagattt
     3481 tgagcttctt caaatcgcat ctcttgtgca gcttttagca tggattcttt aaactctttt
     3541 tttaatcttg aagtctctcc ttttagaatt gatttaactc catcaatttg ttgcatatat
     3601 ctatgatcag aaatgtatcc agcacaaggt gctttgcatt ttccaatatg ataattaaga
     3661 caaggcctga aattctttgt ttttatattt gattctgtta gtgacagttt acagtttctt
     3721 atctgatata atgtattaat caggtcaatc agactgtgag cgtacattgc agaactataa
     3781 ggtccaaaat agagagaacc gtctttactg tattttcttg ttacaaagac tcttgggaat
     3841 ggttcatttt ttatgcagat ccatggataa gtcttactgt cttttagaag aatgttatat
     3901 cgaggctgat atttctttat caaattattt tcgagaagta atgcttcttc ctctgaatta
     3961 acaaccgtat actcaatatg atcaattttt gagaccatgg ctttagtctt gggagagaga
     4021 gtgttttcag actggaaata ttgagagata cggtttctga gattttttgc cttgccgata
     4081 tatataatta ttccttcctt gttaacaaaa cggtaaactc cgggttgtgc tggaagtatt
     4141 ttgagagtct ctcttatatc gggtcgttgg atcatagttt ttcgtaaaat taacaattat
     4201 cctatatttg tgatatttta tgaatagagt agtaacaaaa tctgacgtta aaaaggcaat
     4261 aaaacttaaa ggatttcccg gtaatattgc agcatctttt ttgatgtgga ttttaggttt
     4321 aaatacgctc aataaaaaat acagtaaaat ttgtcagttt gaggggcggg attttactac
     4381 tgctgttatg agagaatttg ggtttggata tgaactcgat gacagacaac ttaaaaatat
     4441 tccacaggaa ggccccttta ttttagtttc caattttcca tacggttcaa tagagggaat
     4501 aatattattg catatcgtct ctgctgtaag gccagaaatt aagtttctgg gaaatttcat
     4561 gttatctata attcccaatt tgcgcgatta tattttcccc attaatcctt ttgtagatca
     4621 tcctgagtta cataatagtt tttctgggct cagacaggcc agtgaaactc tcgcaaacgg
     4681 aggctgtctt ggactttttc ctgccggaga agtttctact tattatggag gaatatttat
     4741 tcaggataaa gagtggcaga tatctattat caaattgatt aaaaatgcgg gcgtacctgt
     4801 aattcctgtt tactgtcatg gtactaactc gcgttttttt cactgggcgg gtaggatcca
     4861 tccgttgtta aggacactca gacttccaaa ggagctttta aataaaagag gtaaaattgt
     4921 ttcagtaaga attggaaagt cagtctcttc ttatgaaata tcagaatata aagatcttaa
     4981 atctttggga gcatatctta aagctcgcac atatgctctt gaagctaata ttaaaacagc
     5041 ccaaaaaatt gaagccaggg cacttactcc tattgcatta cctaataaca gaaaacattt
     5101 acaaaaggaa attaatactt taccacaaaa ctgtctgctt ttttcaaccg gcaattatgt
     5161 ttgttatctt gctgattata agcttatacc tatgatgatg cacgaattag gcaggaggag
     5221 agaagagtct ttcagggcag taggtgaggg taccgggaaa ccacttgatc tcgatgaata
     5281 tgatgagtac tataaacatt tgattttgtg ggataaatat agacaacgtc ttgttggtgc
     5341 ctatcgtttg ggctttggta aaaatataat tgaaacaagg ggagtaaagg gtttatactc
     5401 aaattctctt tttggctatg aaaagccgtt tcaggacgta cttaaacagt ccatagaact
     5461 tggccgctca tttgtgacac ttgactatca aaaagataac ctttcattaa tgttgcttat
     5521 caagggactt ttatattctg tgctcagtca tcctgaagtt aaatacctga ttggtcctgt
     5581 aagtattagc tcctggtatc ctaaatttta tagaagtgtt atgatttatt atattcaaaa
     5641 caaacactct ataaatgatt ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnngaaagac gttcgacttt tcgtaatttg caatcacttt tgttcaaagt aaatagaaag
     5821 tgctttggca attgatggag cttcaggtgt tggggctatg acagtagatg tcaactttgc
     5881 tcctttgaga gccttgagtg ttgccggacc aaatgtggca aactggatgc cgtttggttt
     5941 aaaatcagga aaattctgtt taagggatct tatgtcagaa gggctgtaga aaacgagcat
     6001 atcataatta ttcaactcta aattactaag atcactgaat acggttttga caaatactcc
     6061 gcttgtatgt ttgagtttgg cctttacaaa tattttatga atatctgcct ttgacgaatc
     6121 tgttacacaa acaagaaaat tttcattttt atgtttggtt ccaatagcat caattattga
     6181 ggcatttgtg ccattgccaa aaaatatttt tctttttcgg taaacaatat atttttgcaa
     6241 atagagggca atggactctg aattacaaaa atacttcatg gtttcaggta ctgtaatcct
     6301 catctcttcg caaattttaa agaaagcatc aatagctgtt ttggctgtga atactactgc
     6361 ggtgtgatca aggatattta ttttttgaag gcgaaactct tttgcctgta atggttcaac
     6421 tttgaaaaat ggtacaaagt cgatacttaa cttatatttt gttataagat ctgtgtaggg
     6481 agatgatcca tttgttggag caggttgaga aatcaggatc cttttggctt tcatatttta
     6541 tatagtgaga attaaatcaa cggttaatgc aattggtaag aattccaaag tgcaaagata
     6601 caaaatataa aaaaacagag aaaattggcg agatcttatt acttgccatg ttttgaccaa
     6661 atagatgaag tacacaaata aaacacagta aataagcaag ttaatgaata atttattgtc
     6721 taactcaggc cagaaaaaac ttgccagcga tattgggaaa gtgaaaaaga cggaaatgat
     6781 aaaataatta taacttattt tttcaataaa attaaacgca tagttctcat ttaatagcca
     6841 tgagagtaat gcaagagcac cctttcgaac taaccataaa caaattactg ttcctaataa
     6901 aatcaaaaaa aatagtggag gagtgatatt atattttgta acaatttgtt ctcttaaagt
     6961 aagggagagc atgagaggat aataataaaa gaatataccg accacaatat ttctttgatt
     7021 ggagatagca agtctttctt caattttttc gtgattttta agtttaaaaa aagattttat
     7081 aattgaaggg taaacattaa taatttcctt tgaaaaaatt ataagtacaa tgaaaaaagc
     7141 aagaaaaagc cccgacataa tactgtcatt ttctgacttt tctcttcgtg gtgttgtgta
     7201 attactacct gttatgtaaa atgtatcctt tccccacagc gaatctacag gtagagtttg
     7261 aatagaatca ggtatccagg aatagtcata aggatatgca caggtctgct ccatcaatta
     7321 ccacgttttg ctttataatt catgccttgg agaatgttga ttagtttgtc acgaaaatct
     7381 ccttgaataa ttatttcacc ctctttggct gaacctcctg ttccacactt gtttttaagt
     7441 attttggcca atgactcgag atcctcttta gaaccttgaa atcctttaat aagtgttact
     7501 tgctttcctg ctctctgttt ccgatctata gcaactgtca aatattgttt ttcgggcttt
     7561 aacgtatgaa tagcatcgtc tttattgttg tcttttgtat agttgaagtc aggatctgtt
     7621 gagaagacga ttccaagtct gctcttccaa tcattctgag acattgctga tttttgttac
     7681 aaagttaggt agaaaaattc aaaatagtaa ctttgtttat tattagcaat aattaggcaa
     7741 aaacaatatg gagcaaaaaa aatttaaact gataaataac ttgctaggat tactggtttt
     7801 tatcattgct gcattcacct atctttcgac tattgagcct acggccagtt tttgggattg
     7861 tggtgaattt attgcatcat catacaagct tgaaatagga catccaccag gaaatcccgt
     7921 ctttcagcta attgcccgat tttttactct ttttgctgat aaccagcatg cggcgatgat
     7981 ggttaatgca atgagcgcta tttgttctgc tttgacgata atgtttttgt tctggacaat
     8041 tactcatttg gtaaggagaa ttgttgagaa gcataaagag actgtgactg cagctacagc
     8101 tatttcggtt tttggcgcag gtctggttgg ctctttagct tatgcttttt ccgacacttt
     8161 ctggttttca gctgttgagg gagaggtata tgccatgtct tctttattta cggcagttgt
     8221 tttctgggct atgttaaaat gggaagagtg cgccgacgaa cctcatgcca acagatggat
     8281 agttcttatt gctcttttga tggggctttc catcggagtt catttgttga acttacttac
     8341 tattcctgct cttgtgttta tatattatta taaacgatac aatgttacta acaaagggct
     8401 tgtctgggta tttgtgcttt cttgtgtaat tctggccttt attctttacg gaataattcc
     8461 gttcttacca aaaatagctg cttggtttga cctctttttt gtcaatgtgc ttggctttgg
     8521 catcaatact ggtgcagcta tatttcttct gttgcttctt atagcttctt tgtggtcagt
     8581 gtggtataca tataaaaaag gcaaagtttt ggccaacacg attttacttt gttttactat
     8641 gattgtgata ggttattcga catttgccgt tgtaattatc agatcatctg caaatactcc
     8701 aacaaatgag aatcaacctg ataatgcttt ttcacttgta agataccttg cacgtgagca
     8761 gtatggcagt aatcctctta tttatggcga gacattcgct tcgccatatg atatcaaaca
     8821 ggaatcgtat tatgctaaag tggataataa atataaaaag gttgagggac ctgttcagcc
     8881 agtatatgat cctgcttcaa aaatggtatt tcctcggatg tggagttctg cttctgatga
     8941 tcatgtgaaa ttttataaga tgtatacaga ggggagagga aaatcgatag tcggatcaga
     9001 cgaaaagatt ccattgatga aagataatct tgctttcttt tttgactacc agcttaactg
     9061 gatgtatttg cgttatttta tgtggaattt tgcaggtcgt caaaatgatc ttcatggttc
     9121 tactccagga gatcttctca gggggaattg ggagagtggg attactttct ttgacagagc
     9181 aagacttgga gatcagagcg aaggtcctgc atacattgta aaaagcaagg ccaaaaatca
     9241 ttattacatg attcctttgt ttctgggaat attaggcttt ttttatcagc ttgccaagga
     9301 caaaagaaat acctggattg ttacactttt gtttattctt acgggcgtag ccatagttgt
     9361 atatcttaat caaacgccat atcagcctcg tgagagagac tatgcttatg ccggatcttt
     9421 ctatgctttt gctatctgga tagggcttag cgttactgct ctttatgatg ctttcaggaa
     9481 gagaatcccg gcattagtga cagcttcagc ggtaacactt gtaactcttt gtgtacctgt
     9541 tctgatggct tgcgaaaatt gggacgatca tgatagaagt aacaggtaca gtgcgcgaga
     9601 tcttgcgtat aactatttga attcttgcga taaacaggca atacttgtta cacatggtga
     9661 taacgatacg ttccctttgt ggtatattca agaggttgag ggagtcagaa cagatgtaag
     9721 aataatgaat acttcactcc ttggaacaga ttggtatatt gatcagatga aacgtaaaac
     9781 atatgaatcg gaaccggtca aactctcatt gccgaggagt gagtatcttt atggtacaaa
     9841 tgatatgatt catattgttg acagattgaa caggccggta cccattaaag cagtgataga
     9901 tctgatagcc aaccccgaag ctaaattaca ggctgaaaat ggaaagagat atagttttgt
     9961 ggcttcaaag caattgcttc ttcctgtcaa cgtaaaaaat gccattgcct gtggaattgt
    10021 ctccgaggct gattctgcgc aaatactgga tactttgttg cttaagatac ctgaaaagaa
    10081 gaattatctt acaaagtgtg aaatgattat tcttgatatg ctggcaaatt acgattggag
    10141 cagaccgatt tacctggttt caatgggggg agatcttgaa cttggattga gggattatct
    10201 tgaatataac ggttttgcct ataaatttgt gccgattaag agctcaacat ctcttggtag
    10261 tccgggaaga gttgttccgg aagttatgta tgacaagtgt atgaatgtat atcgttgggg
    10321 caatttaaat gatcccaaag taaatgtgga ctaccagaat ctgctcacat tcaatgcctt
    10381 gatgtgtgga agaaatattt attctacaac tgccagatca ttgcttgagt gtggaaagac
    10441 taaggaagct gttgaggttc tggataaaat gcaggaatat tttatccctt cacaatttcc
    10501 tcttaattgc tctcttttac catcacttaa cgaaatatct gtgatggagg caatagatat
    10561 ttatctccaa gccggcgaaa gagacaaggg attagctctt gttaaagcgt ttacggacga
    10621 aacatatcag gccttgaatc ttttcactaa g
//
LOCUS       DAOTQL010000051        10591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5640_length_10591_cov_5.038345, whole genome shotgun sequence.
ACCESSION   DAOTQL010000051 DAOTQL010000000
VERSION     DAOTQL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_10591_cov_5.038345"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="P5293_04325"
     CDS             <1..1503
                     /locus_tag="P5293_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRR49162.1"
                     /translation="DEKGILKAGFKTMDPVKELKLEDRHIVYAAITSLTKESLKDSGL
                     DTKAILRCKNMFTLGMCFYMFNRPLELTFAYIDKKFRKKPEIAEANKKALKDGFNYAS
                     NIQAIPNTYIVQPAKLEKGIYRNINGNQATAWGLLAASEKSGRPLFCGSYPITPATSI
                     LEELALRKDLGVKTLQCEDEISGICTAIGAAFAGNFAVTSTSGPGFSLKTEALGLAMI
                     TELPLVVVDVQRSGPSTGIPTKTEQTDLNQALYGRNGECPIAVMAAHSPSHCFEAAFY
                     AGKYAMEHMMPVILLTEGFLGNGSEPWKIPSMKDLPDINPPIITTPEETPFWPFKRDP
                     ETMVRRWALPGSIGLEHRVGGLEKNNNGAVTSDPLVHERMVSERAEKVRRLSNFIPEQ
                     KVIGKEKGDVLIVGWGGTFGHLYTAVKEFHEQGKSVSLAHFDFIFPLPKNTGEIFSNF
                     KKIIVCELNSGQFANYLRTQYQQFHYYQYNKVQGQPFIVKEIVDAVNAIL"
     gene            1519..2544
                     /locus_tag="P5293_04330"
     CDS             1519..2544
                     /locus_tag="P5293_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRR49163.1"
                     /translation="MMELKYTPQDFKSNQEVKWCPGCGDHAVLASVQRAMPDICEALN
                     YTRERFVFVSGIGCSSRFPYYMNTYGFHGIHGRASAISTGVKVANPYLTVWQITGDGD
                     ALAIGGNHFIHAIRRNIDINIILFNNQIYGLTKGQYSPTSKLGMVTKTSPYGTVEHPF
                     NPGELVLGARGTFFARSLDVELKLTQDIMVESAKHDGTSVTEVLVNCVIFNDKTHATI
                     SDKETRADRTIILKHGEPMIFGKNRDKGLILVGRKLKVVKIGEGGITEKDILIHDAEE
                     ENPGLHSMLINMKYPEFPVALGVIRSVKDKTYDDNVRDQVIDVREHAQIHNMDELLHS
                     GSTWEVK"
     gene            2586..3923
                     /gene="gdhA"
                     /locus_tag="P5293_04335"
     CDS             2586..3923
                     /gene="gdhA"
                     /locus_tag="P5293_04335"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRR49164.1"
                     /translation="MKVADIMAELERKHGHEKEFLQAVREVLESIEEIYNQHPEFEKA
                     KIIERLVEPDRIFTFRVSWIDDKGEIQNNLGYRVQFNNAIGPYKGGIRFHPSVNPSIL
                     KFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSDNEIMRFCQAFITELWRHLGPDTDV
                     PAGDIGVGGREVGYMYGMYKKLARENTGVFTGKGSTFGGSLIRPEATGFGAVYFVTHM
                     LKTKGIDIKGKRVSVSGFGNVAWGAALKATQLGAKVVAISGPDGVIVDEDGLNDEKIA
                     YMLELRASNNDVVAPFAAKFPRAKFIPGKKSFSVKVDIAMPCAFQNELNGDDAKELIK
                     NGVTCVAEVSNMGCTPEAIEEFIKAKILFAPGKAVNAGGVAVSGLEMTQNAMHISWTA
                     EEVDAKLKQIMNSIHDACIKYGKQSDGYINYVKGANIAGFMKVAQATLEQGVI"
     gene            4146..7325
                     /locus_tag="P5293_04340"
     CDS             4146..7325
                     /locus_tag="P5293_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49165.1"
                     /translation="MKKIKLFLTGLLFVLTAGLSFAQNRQITGTVIDASTGEPVAFAS
                     VQLKGTMTGTTTNESGKYTINAPGNGTLIFSFIGYTTVEVAINNQNVVNVSLSPEAVN
                     LEDIVVVAYGTAKKSSFTGSASTVKGSKIAERSVSNVTNALAGQVAGVQVTSNNGQPG
                     TAATIRIRGIGSMSASNAPLYVVDGVPYDGTISAINPQDIESLTVLKDAAASAIYGAR
                     GANGIVLITTKKATSKDAIVTFDAKWGTNSRAIPNYDVMTDPAMYYETLYRALYNAKA
                     YNGSSAAAAYAYADVALLDDKNGGVGYMVYTVPGGEKFIGTNFKLNPRATLGYSDGEY
                     YYIPDNWYDEIFDKGNLRSEYNFSVSGSTDKLNVYLSAGYLNDSGIVSGSGFERFTTR
                     IKADYQAKEWLKIGANTAYTYYDSKAPGSQTDWGSSGNLFYVTNMIAPIYPMYVRNAD
                     GTIKVDNRGITVYDFGGSSTNFTRSFMALANPAITLKLDKYHAYTDVVNSKWYATANI
                     IEGLQFTATVGANIRNQRSNNLYNQFYGASVGSQGAVSVRHYREFGVNQQYLLTYKKN
                     IATNHNIDLLVGYESYNLKLQNLGGSNTKLYNPYIGELSNAIQIPPSVYSYTDTYTTQ
                     GILARAQYDFKEKYFLSASYRRDASSRFHPDKRWGNFGSIGGAWLISKENFMSSINWI
                     DMLKIKASYGIQGNDNLGNYYAYLDQFTVTNSSGDFSVAFAYKGNKDITWETSYAFNG
                     GVDFELFGSKLTGTVEYFSRKTVDLLYNQPVPISLGYSSIPMNVGSIVNNGVELELNG
                     TILKTNNILWTANFNATHYKNKITDLAETPKKAGGIKGSNYIYRIGGSLYNSYLRKWA
                     GVDPETGKSLYYVDPDKGDMTTTDDYTKAVQCDLGSTLAKVYGGFGTTINAFGFDLSI
                     QLSYQLGGKLYDGSYEQLMHQGDDPGTNWSMDILKAWTPEKPSTKYPRLDASIDTYQK
                     QSSRFLTSSDYLSFNNVMLGYTLPKNLVKSLKLSTVRIYVSGDNLGVISARKGLDPRQ
                     SLGLGSSTTSGNYSYSAMRTISAGISLSF"
     gene            7353..9002
                     /locus_tag="P5293_04345"
     CDS             7353..9002
                     /locus_tag="P5293_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR49166.1"
                     /translation="MKLNKKLYVFALGGLLFTSCVDLNTAPEGGTFTSEQKSEVVLAL
                     PQRLAADVNGMFASIGKQYCVFGTASSRHDDAGYPTVCLSQDLNGPDMVSDNSNYNWF
                     SVSSSYEDRNDTYANPYMRWAVFYNQLKLANDILATIPADTDDPTLKIYQAQARAIRA
                     FDFLSLVPYYQFKYKGHEDQPSVPIITENMEGDPSNNPRAPLSAVYELIISDLDAAIT
                     ALAGYTRTSKAEIDQKVAYGLRARANLYMENWAAAASDANNAMAGYSPYTRAQVSKPT
                     FIDANDQNWMWAIILTPTNIPDPYPSWPAVLGSFSGDSYSAAVGCYKSVNRLLFDKIP
                     STDVRKGWWVNENLESPNLATVTWGGVTGNAVAKLAITDVKLPFVPYTNVKFGQYGFI
                     GNNINAGDWCIMRVEEMILIKAEATAMAGNLSAGKQILEDFVKTYRDPSFVSTATSAE
                     DFQNEVWLQRRIELWGEGFSMSDIMRLGKNVVRFTPSITSNFPDAFKFNLASNDGWLL
                     LRIPQRETNNNRGIPLSANNNEGTLPVSGAGSGLTDGVIVH"
     gene            9027..9860
                     /locus_tag="P5293_04350"
     CDS             9027..9860
                     /locus_tag="P5293_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49167.1"
                     /translation="MKLHNKFKFGILLTSISLLLSATACEDEVKREPSPETNPNSSNV
                     YFASSNSSSVVLGVSATNFEVIVAREKTAAALTVKLKTESIYGDLFTVPESVSFAAGE
                     AQKSISIACGDLDLMKTYHFAIEIDPNETKPYVAQNVYPRIELNVLKEDFQPYANGLW
                     WDLFFAPGNDYISWPLTLEYSEMSQTYRLKETWGYPGYDITFKWDGGANVTMGGTASG
                     SYKVFKTGYVDPTYGMVSAYFGTCTYNAATKTFTFPITWRVSAGSFGLYPDYYEIKTL
                     L"
     gene            complement(9938..>10591)
                     /locus_tag="P5293_04355"
     CDS             complement(9938..>10591)
                     /locus_tag="P5293_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRR49168.1"
                     /translation="RIGETTIDVAGKVVNVAEATIPDLVAYYQINLMNPKVLTGVFIG
                     SMMSFLFCGLTMNAVGRAAQKMVEEVRRQFREIAGILEGKAKPDYAKCVEISTKGAQH
                     EMLLPSLLAIIVPVLVGFIFDVPGVMGLLVGGLSTGFVLAIFMANAGGAWDNAKKYVE
                     DGNFGGKGSEAHKATVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVIAGLTVAFHLF"
BASE COUNT         3258 a         2154 c         2140 g         3039 t
ORIGIN      
        1 gatgaaaagg gaatacttaa agcaggtttc aaaacaatgg accctgtcaa ggagcttaaa
       61 cttgaggaca gacacattgt ttacgcagct attacttccc tcacaaaaga gagtcttaaa
      121 gatagtggtc ttgataccaa agctatactt cgctgtaaaa acatgttcac gcttggcatg
      181 tgcttttaca tgttcaacag acctttggaa ttgacttttg cctatatcga caaaaagttc
      241 cgtaaaaaac cggagattgc cgaggccaat aaaaaagctc ttaaagatgg cttcaattac
      301 gcaagtaata ttcaggctat cccaaacaca tatattgttc aaccggcaaa actggaaaag
      361 gggatttaca gaaatatcaa tggcaatcag gcaacagcct ggggtttgct tgctgcctca
      421 gaaaaatccg gcaggcctct tttctgtggc tcctacccta ttacaccagc tacaagcatc
      481 cttgaagagc tagctttgcg taaggatctt ggtgtcaaaa ctcttcagtg tgaggacgaa
      541 atttctggaa tatgtaccgc aattggtgct gcatttgctg gcaacttcgc cgttacatct
      601 acttcaggtc ctggattttc tcttaagaca gaagcactcg gtctggctat gattactgaa
      661 ctacctcttg ttgttgtaga tgttcagaga agtggccctt caacaggcat ccctaccaaa
      721 acagaacaga cagaccttaa tcaggctctt tatggaagaa atggtgaatg tcctattgct
      781 gtcatggctg ctcattcccc atcacactgt ttcgaagctg ctttttacgc cggaaaatat
      841 gctatggaac acatgatgcc agtaattctt cttacagaag ggttcctcgg taatggatca
      901 gaaccatgga agattccgtc aatgaaagat ttaccagata ttaaccctcc aattattacc
      961 actccagaag agacaccatt ctggccattc aaaagagatc cggagactat ggttcgtcgt
     1021 tgggcattgc caggatcaat tggacttgaa cacagagttg gcggactgga aaaaaacaat
     1081 aacggagcag ttacctctga ccctcttgtt catgaacgaa tggtttccga aagagcagaa
     1141 aaggtaagaa ggctgtccaa tttcattcct gaacaaaaag tcattggaaa agaaaaaggg
     1201 gacgttttga ttgttggctg gggaggaacc ttcggccatc tttacacagc tgtcaaagaa
     1261 tttcatgaac aaggcaaaag tgtttcactg gctcattttg acttcatttt ccctcttcca
     1321 aaaaataccg gagaaatctt ctccaatttc aagaagataa ttgtatgtga acttaacagt
     1381 ggccaatttg caaattacct aagaacccaa tatcagcaat tccactatta ccaatacaat
     1441 aaggtgcagg gacagccatt cattgtcaaa gagatagtgg atgcagtcaa tgctatatta
     1501 taacttcaat aaagaattat catggaactt aaatatacac cacaagattt taaaagcaat
     1561 caggaagtta aatggtgtcc tggttgtgga gatcatgctg ttcttgcttc cgtgcagaga
     1621 gctatgcctg atatctgtga agcccttaac tacactagag agcgtttcgt atttgtatcc
     1681 ggcattggat gttcttcacg tttcccttat tacatgaata cttatggttt tcatggtatt
     1741 catggcagag cttcagctat ttccacagga gtaaaagtgg caaatcccta tctaacagtt
     1801 tggcaaatta caggtgatgg agatgctctt gctataggcg gcaatcattt tattcatgca
     1861 ataaggagaa atattgatat aaatataatc ctcttcaaca accaaatata tggcttgaca
     1921 aagggacagt actcgccaac ttcaaaattg ggaatggtta ctaaaacatc cccatatggt
     1981 acagtcgaac atcctttcaa tccgggagaa cttgtattgg gagcaagagg tacatttttt
     2041 gcgagaagtc ttgatgttga actaaaactt actcaagata taatggttga atctgcaaaa
     2101 cacgacggta catctgttac tgaagtactt gtcaactgtg tcatcttcaa tgacaagact
     2161 catgctacta tctctgataa agagacacgc gccgacagaa ctattattct taaacacggt
     2221 gagccaatga tatttgggaa aaatcgtgac aaggggctca tccttgttgg aagaaaactc
     2281 aaagttgtca aaattggaga aggaggaatt acagaaaaag atatccttat ccatgatgct
     2341 gaagaggaaa atccgggact tcattcaatg ctaatcaata tgaaatatcc tgaatttcct
     2401 gttgcccttg gagtcataag gtccgttaag gataaaacat acgacgacaa tgttcgtgat
     2461 caggtcatcg atgttaggga gcatgcacaa attcacaata tggatgaact ccttcacagt
     2521 ggatcaacct gggaagtaaa ataaaatatt taattaaata ccaaccaatt actaaaaaaa
     2581 caattatgaa agtagcagac attatggccg aactcgaaag aaaacatggc catgagaaag
     2641 agttcttgca agcagttcgt gaagtcctcg aatctattga ggaaatttac aatcaacatc
     2701 cggaatttga aaaggcaaag attattgaaa ggcttgttga gcctgaccgt atcttcactt
     2761 tccgcgtttc ctggatagac gataagggag aaatccaaaa caatcttggt taccgtgtac
     2821 agtttaataa tgccattggg ccatataagg gaggtatccg tttccatcct tcagtcaatc
     2881 cttcaattct caagttcctc ggttttgaac aaacattcaa aaatgctctt acaaccctac
     2941 caatgggtgg aggaaaggga ggatcagatt ttgatcctaa gggaaaatcc gacaatgaga
     3001 tcatgcgttt ctgccaggca tttatcactg aactctggcg tcatttaggc ccagacactg
     3061 acgtgcctgc aggtgatata ggagtaggcg gacgagaagt tggctacatg tatggaatgt
     3121 acaagaaact ggctcgcgaa aacactggcg tattcacagg caaaggctcc acttttggag
     3181 gttctctcat tcgtcctgag gccacaggat ttggggctgt ctattttgta actcatatgc
     3241 tcaaaaccaa aggtatagac atcaaaggca agagagtgtc agtttcaggt ttcggaaatg
     3301 ttgcatgggg cgctgccctc aaggctactc aacttggcgc aaaagtggtt gcaatttccg
     3361 gtcctgatgg agttatcgta gacgaagatg gattgaatga tgaaaaaata gcttatatgc
     3421 ttgagctccg tgcctcaaac aatgacgtag ttgcaccatt tgccgctaaa ttcccgaggg
     3481 caaaatttat ccccggtaaa aagtcattca gtgttaaagt tgacattgca atgccttgtg
     3541 cattccagaa cgaacttaac ggagacgatg caaaagaact gattaaaaac ggggttacct
     3601 gtgtcgcaga ggtttctaat atgggttgta cccctgaagc tattgaagaa tttatcaaag
     3661 ccaagattct atttgcccct ggtaaagctg tcaatgcagg tggtgtagct gtttccggtc
     3721 ttgaaatgac tcagaacgca atgcatataa gctggactgc agaagaagtt gatgccaaac
     3781 tgaaacagat tatgaatagc atacacgatg cttgcatcaa atatggtaaa cagtctgacg
     3841 gctatattaa ctatgttaag ggtgcaaata ttgccggctt tatgaaagta gcacaggcca
     3901 cattagagca gggggttatt taataacaaa ctgcttaaaa ttgtgtttta ggaaagaggg
     3961 tgcccaaaaa gtaattttgg gcgcccttat ttattcttta atttatgcag accttttttt
     4021 attaataatc aacttaatga taatataaaa tttaatacaa taattttaat ttttcaaaaa
     4081 aaaaattaac tttgttaaaa ctaattctta atcataaact taattactaa ctaaaacttc
     4141 cttacatgaa aaaaattaaa ctatttttga caggtctgct gtttgtgctg actgctgggt
     4201 tgtcttttgc tcagaacaga caaattacag gtactgtcat agatgcaagt acaggggaac
     4261 ctgttgcttt tgcatctgtt cagctgaaag gtaccatgac aggaactacg actaatgaat
     4321 ccggtaagta taccataaac gcccctggta atggtacttt gatttttagt ttcataggct
     4381 acactactgt tgaagttgcg ataaacaatc agaatgtagt taatgtttcc ctctctcctg
     4441 aagctgttaa tcttgaggac attgtggtcg tagcatatgg tacagctaaa aaatcttctt
     4501 ttaccgggtc tgcgtcaaca gtcaaaggaa gcaagatagc agaacgttcg gtttccaacg
     4561 ttaccaatgc tcttgccggt caggtagccg gtgttcaggt taccagcaac aatggacaac
     4621 ctggtacagc tgctacaatc agaattcgcg gtatcggttc catgagcgca agcaatgcac
     4681 ctctatacgt agttgacggt gtaccttatg atgggactat atctgccatc aatccacaag
     4741 atattgagag tcttactgtt cttaaggatg ctgctgcaag cgccatctat ggagcacgtg
     4801 gtgctaatgg catagttctt atcaccacaa agaaggcaac ttccaaagat gctatagtta
     4861 ctttcgacgc caaatggggt acaaactcca gagctattcc aaactacgat gtaatgacag
     4921 atcctgccat gtattatgag actctgtata gagctttata caatgcaaaa gcttataatg
     4981 gctcatctgc cgctgctgct tatgcttatg ctgatgtagc tcttcttgac gacaaaaacg
     5041 gtggagttgg ttatatggta tacactgtac caggtggtga aaaatttatt ggtactaact
     5101 ttaaattaaa tccaagagcc acacttggtt attccgacgg tgaatactac tatatacctg
     5161 acaactggta tgatgaaatc ttcgacaagg gtaatcttcg ttcagaatac aacttctctg
     5221 tctctggttc aacagacaaa ctgaacgttt atctctctgc aggttatttg aacgattccg
     5281 gtattgtatc aggatcaggt ttcgagcgtt ttactactcg tataaaagct gactatcagg
     5341 ctaaggaatg gttgaaaatc ggagcaaata cagcttacac ttattatgac tcaaaagctc
     5401 cgggtagtca aactgactgg ggatcctcag gcaacctgtt ttacgtgact aacatgattg
     5461 ctcctatata tcccatgtac gttcgtaatg cagatgggac catcaaagtg gacaacaggg
     5521 gtataactgt ttatgatttt ggagggtctt ctaccaattt tacaaggtct ttcatggcac
     5581 tcgcaaaccc tgcaattact ctgaaactgg ataagtatca cgcctataca gacgttgtta
     5641 acagcaagtg gtatgcaaca gccaacataa tcgaagggtt gcaatttaca gctacagttg
     5701 gtgcaaacat taggaaccag cgttccaaca atctttacaa ccagttttac ggtgcctctg
     5761 taggcagtca gggtgcagtc tctgtaagac actaccgcga attcggagtt aatcagcaat
     5821 atcttttgac ttataagaaa aatattgcaa ctaatcataa tattgatctc cttgttggtt
     5881 atgaatcata caatcttaag ctgcaaaatc tcggaggttc aaatactaaa ctgtataatc
     5941 catatatcgg cgaattgagc aatgctattc agatacctcc ttctgtctat tcatatacag
     6001 atacatatac aactcagggt attcttgcaa gggctcaata tgattttaag gagaaatatt
     6061 tcttaagcgc ctcttatcgt agggatgctt cttcacgttt ccatcctgac aaacgttggg
     6121 gtaattttgg cagcatcggt ggtgcatggc tcatcagcaa agagaatttc atgagcagca
     6181 taaactggat agatatgctc aagattaaag ccagttacgg tattcagggt aatgacaatc
     6241 tcggaaacta ttatgcatac cttgaccagt ttacagtaac caacagtagt ggggatttct
     6301 cagtagcatt tgcatacaag gggaacaaag acatcacatg ggaaacatcc tatgcattta
     6361 atggaggagt ggattttgaa ctttttggaa gcaaattgac aggtaccgtt gagtacttca
     6421 gccgtaagac tgtagacctt ctctataacc aacctgttcc aatttctctc gggtactctt
     6481 caatcccaat gaacgtcgga tctattgtta ataatggagt tgaacttgaa ttgaatggaa
     6541 caattctaaa gactaacaac attctatgga cagccaactt caacgccact cattataaaa
     6601 ataaaattac agaccttgct gaaacaccta aaaaagcagg tggaattaaa ggcagtaact
     6661 acatttaccg cattggaggt tctctctata actcatatct gcgtaaatgg gcaggagttg
     6721 acccggaaac tggcaaatca ctttattatg tagatcccga taagggagac atgacaacaa
     6781 cagatgacta tacaaaagct gttcagtgcg acctaggctc tacccttgct aaagtttatg
     6841 gtggatttgg tacaactatc aatgcatttg gatttgacct ctcaattcaa ctgtcatacc
     6901 agttgggtgg caagctgtat gatggttcgt atgaacagtt gatgcatcag ggagatgacc
     6961 ctgggactaa ctggtctatg gacattctca aggcctggac acctgaaaaa ccttccacta
     7021 aatatccaag gcttgatgct tctatagata cttaccaaaa gcaatcttca agatttctta
     7081 caagttcaga ttatctgagt tttaacaatg tcatgttagg atacaccctg ccaaagaatc
     7141 ttgtaaaatc cctcaaattg tcaactgtcc gcatatatgt ttcaggtgac aatcttggag
     7201 ttatttctgc ccgtaagggt cttgacccgc gccaatcttt ggggcttggc agttctacaa
     7261 cttccggaaa ctatagctac tcagcaatgc gtacaatatc tgccggtatc tctttatcat
     7321 tctaagtctg acttaactta attataatca ctatgaaatt aaataagaaa ttatatgttt
     7381 ttgctttagg aggtctcctg ttcacttcat gtgttgacct taatactgct cctgaaggag
     7441 gtacttttac atctgagcaa aaaagcgagg tagtgcttgc attgcctcaa agactggctg
     7501 ctgatgtgaa tggtatgttt gcaagcattg gaaagcaata ttgtgttttt gggactgctt
     7561 cgtccagaca tgatgacgca ggttatccga cagtatgcct gtcacaggat ctcaatggcc
     7621 cggacatggt tagtgacaat agtaactata actggttttc agtcagtagc agttacgaag
     7681 acagaaatga cacatatgca aatccataca tgcgatgggc tgttttctat aaccagctta
     7741 aacttgcaaa tgacattctt gcaaccatcc ctgctgatac agacgatcct actttgaaaa
     7801 tctatcaagc ccaggcgaga gcaattagag cttttgactt cctgagtctt gttccttact
     7861 atcagttcaa atataaaggg catgaagatc agccttcagt tcctataatt acagaaaata
     7921 tggaaggtga cccttcaaac aatcctcgtg caccactttc tgcggtatat gagcttatta
     7981 ttagtgactt ggatgctgca attacagctc ttgccggtta tacaagaaca agcaaggccg
     8041 aaatagacca aaaagttgct tatggtcttc gtgcacgcgc caatctgtat atggaaaact
     8101 gggcagcagc agcgtctgat gcaaataatg caatggctgg atactcccct tataccagag
     8161 ctcaggtttc taaaccaaca tttattgatg caaatgatca gaactggatg tgggcaataa
     8221 ttttaactcc gacaaacatt cctgatcctt acccgtcatg gcctgctgtt cttggttcat
     8281 tttccggcga ctcttactca gcagctgttg gctgttacaa atctgtcaac cgtctgttgt
     8341 ttgacaagat tccttctact gacgttcgta aaggatggtg ggttaacgaa aaccttgaat
     8401 caccaaatct tgcaactgtt acatggggcg gagttacagg caacgctgtt gctaaacttg
     8461 ctattactga tgtcaaactt ccattcgttc cttacaccaa tgttaaattc ggtcaatatg
     8521 gatttatagg aaataatata aatgctggcg actggtgtat catgagagtt gaagagatga
     8581 tattaattaa ggccgaagcc acagctatgg ccggtaacct ttctgcaggc aaacaaatac
     8641 ttgaagattt tgtcaagaca tatcgtgacc cttcatttgt aagcactgct acttctgcag
     8701 aagactttca gaatgaagta tggcttcaac gccgtattga actttgggga gaaggcttct
     8761 ccatgtctga tatcatgaga cttggcaaga atgtagtacg tttcacccca tctattacct
     8821 ccaatttccc tgatgctttc aaattcaacc tcgcatcaaa tgatggctgg ctgctgcttc
     8881 gtattcctca aagggagaca aacaacaaca gaggtattcc actctcagca aacaataatg
     8941 aaggaacact tcctgtttca ggagccggtt caggtttgac agatggagtt attgttcact
     9001 aatactaata attgaaaaag aaaaatatga aactacataa taaattcaaa ttcggaatat
     9061 tgttgacttc aatttcatta ttgttgtcag ctactgcctg tgaagatgag gtgaaacgtg
     9121 aaccatctcc tgaaaccaat ccaaacagct caaatgttta ctttgcctcc tccaacagtt
     9181 catcggtagt tttaggcgtt agtgctacaa atttcgaggt gatagtagca cgtgaaaaga
     9241 cagctgcagc tctgactgta aaacttaaaa cagaatctat atacggagat ctctttactg
     9301 tgcctgagtc agtctctttc gctgctggtg aagcccagaa aagcatttct attgcttgtg
     9361 gagacctgga tcttatgaaa acctaccatt ttgccattga aattgaccct aatgagacta
     9421 aaccgtatgt tgcacagaat gtttatcccc gtattgaact caatgttctc aaggaagact
     9481 ttcaacccta tgcaaatggt ctttggtggg atcttttctt tgccccggga aatgattata
     9541 taagctggcc tttaaccctc gaatactcgg aaatgagtca gacttacagg ctcaaggaga
     9601 cttggggata tccggggtac gatattacat ttaaatggga cggaggagct aatgtgacaa
     9661 tgggtggaac agcaagtggt tcatataaag tttttaaaac aggatatgtt gacccaacat
     9721 acggaatggt gtctgcttac ttcggaacat gtacatataa tgctgcaaca aaaacattca
     9781 ctttccctat tacatggaga gtgtctgcag gtagtttcgg gctttatcct gactattatg
     9841 agataaagac tttgctttaa atctatatta agcctaaaat gattagaggc tgtctaaaag
     9901 taggcagcct ctttttctta ttttacactc ttattaatta aaacagatga aatgctacag
     9961 tcaatccggc aatcacaatt gaaaccatac tcatgagctt aatcagaata ttcaaagaag
    10021 gaccagatgt atctttaaat ggatctccta ctgtatctcc tacaactgtt gctttatgtg
    10081 cttctgagcc cttacctcca aaattaccat cctcaacata ttttttggca ttatcccaag
    10141 caccacctgc attggccatg aaaattgcaa gtacaaaacc tgttgaaaga cccccaacta
    10201 aaagacccat aactccggga acatcaaaaa taaagcctac aagaaccgga acaataatgg
    10261 ccagcaagga aggcaataac atttcatgtt gagctccttt ggtcgatatc tccacacact
    10321 ttgcataatc aggttttgct ttaccctcaa gtattccagc aatttctcta aattgccttc
    10381 tgacctcttc gaccatcttt tgggctgctc ttcctaccgc gttcattgtc agtccacaga
    10441 atagaaaaga catcattgaa ccaataaata ctccagttaa gactttagga ttcataagat
    10501 taatctgata atacgctact aaatcaggaa ttgtcgcctc tgcaacattt acaactttgc
    10561 cggctacgtc tatagtggtt tctccaatac g
//
LOCUS       DAOTQL010000052        10255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_5844_length_10255_cov_4.966176, whole genome shotgun sequence.
ACCESSION   DAOTQL010000052 DAOTQL010000000
VERSION     DAOTQL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5844_length_10255_cov_4.966176"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..560
                     /locus_tag="P5293_04360"
     CDS             105..560
                     /locus_tag="P5293_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49169.1"
                     /translation="MEQTLPLILAYTGLAFMLTLACIGSALGVSICGNSTIGGLKKNP
                     DIFGSSMILTALPSTQGLYGFAAFFLALSRLKSISDLTLSGGLAILAVGIALGFVGLF
                     SAFYQAKLCANGINEMSNGQDVFGKTLILAVFPELYAILAFAAAFLALP"
     gene            651..2042
                     /gene="glmM"
                     /locus_tag="P5293_04365"
     CDS             651..2042
                     /gene="glmM"
                     /locus_tag="P5293_04365"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRR49170.1"
                     /translation="MTLIKSISGIRGTIGGKVGDALTPPDIVKFTAAYVKRLKERIPD
                     KDSYKIVVGRDARISGEMVDKIVCGTLMACGIDVVNAGLASTPTVEIAVVFEGADGGI
                     ILTASHNPKQWNALKLLNENGEFLSSAEGELLLKHAEEEAFDFVSVERLGKCTCKDFT
                     SKHVEAVLSYPLVDIESIYRNGFKVILDAVNSVGGIIMPKLLEAMGVTCIKINCEPTG
                     NFAHNPEPLPEHLKELSTKVVEEGADLGISVDPDVDRLALICEDGTPFGEEYTLVAAA
                     DYILEKRKGATVSNLSSSRALKDITEVAGCVYSAAAVGEINVVNEMKRVNAVIGGEGN
                     GGVIVPDFHYGRDALIGTALILSHIANKKIKASSLRDSYPKYYISKNKIELSDKIDVG
                     YLFEEIKKVFSNNKITDIDGVKIDFDKEKKWVHLRKSNTEPIIRIYAEAPTIEEADSL
                     AKLIIDKIQNIIK"
     gene            2049..3281
                     /locus_tag="P5293_04370"
     CDS             2049..3281
                     /locus_tag="P5293_04370"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRR49171.1"
                     /translation="MFSFRTTPFLDQPDIVLRSGRLGVLCNQTAWLPEKGEYLFETLF
                     KSGRLKRIFTPEHGLFGELQDQERLDSTDSYKALGLNGCEIISLYGSDEKSLTVSASK
                     LNDIDALIVELQDIGSRHYTYTSTLYNLFKVLKQEEINLPIYIVDRLNPAGRQVEGTM
                     LEKNYESFIGIEGIVHRHGLTFGELANMFYNELNAKFPLHIISYKAERAVKELLPWSI
                     PPSPNIPGLFTCHFYSGQVLWEGTNVSEGRGTTRPFEQFGAPFMLNLLSYNQLHGLKN
                     WNDPDNPIFDSAVYIRWQKFIPTFHKYKGEICSGFQLIPVPGMQYHALAHNLKIIRFV
                     AENCSEFQFREGIYERGNDKRAIELLLGDKLLLDYVSGNVDWNQVKDHIKIEEQKWIR
                     KARKFLLYDEQLFRCKQF"
     gene            3295..4047
                     /locus_tag="P5293_04375"
     CDS             3295..4047
                     /locus_tag="P5293_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRR49172.1"
                     /translation="MANPVLTEKVFEKSRVTAGTGAMTIKGTASKSLVMILMILAGAM
                     YTWKVAFQAINPASVQPWMWGGVIGGFVVAMIISFKPIWAQYLAPVYAVLEGLFLGGI
                     SALFSQAFAATAPGIVMNATLLTILTAVSMLLVYRTNIIKVNGKFARIMIIAVGAIAL
                     YYLVSIIVSLFGVDLTLLNDSGPLSIGISLVIIGVAAFSLLLDYEFIARASEAGAPKY
                     MEWYGAFSLMVTLIWLYLEILRILAKFAIGRE"
     gene            4109..4360
                     /locus_tag="P5293_04380"
     CDS             4109..4360
                     /locus_tag="P5293_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRR49173.1"
                     /translation="MKKGIHPENYRLVAFKDMSNDYVFITRSCANTRETIVHDGVEYP
                     LVKVEISNTSHPFYTGKMKLVDTAGRVDKFLNRYKNIKK"
     gene            4439..5155
                     /locus_tag="P5293_04385"
     CDS             4439..5155
                     /locus_tag="P5293_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 domain-containing protein"
                     /protein_id="HRR49174.1"
                     /translation="MESTSEKLIKLAAEYNNLKYFTTDPIIFPRYFSEKLIQGESSLQ
                     DVEIAGILSAHLAWGKRELIVRDCNRMFDEMKWQPYSYIMAGDFKSDSVSLHRSIKWS
                     DFAKICENLRQFYLNNESLECFSPSYIRLNIYGCRDNPNAANKKIHMFRRWMVRNDGI
                     VDLGLWRKISPADLIIPLDTHVHRSSINLGITKRNVSDYKTAVEITEYLRKVFPDDPC
                     LGDFSLFAFTATIGKKNGYF"
     gene            5164..5745
                     /locus_tag="P5293_04390"
     CDS             5164..5745
                     /locus_tag="P5293_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HRR49175.1"
                     /translation="MKNLFIIAGCNGSGKTTASYNILPEMLGCKEYVNADEIARGISP
                     FKPESVSIQAGKIMLARIKELIANGTDFALETTLATRTHANFIRYAQEHGYKVTLVFF
                     WLNLPNLAVERVKMRVESGGHNIDEKVIRRRYDFGLKNLFSLYVPLCEYWMIINNSTL
                     PPELIAEGGKNQETKVYNKPTYNKIVNYERTRD"
     gene            5726..5881
                     /locus_tag="P5293_04395"
     CDS             5726..5881
                     /locus_tag="P5293_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49176.1"
                     /translation="MSEQETKELRDNILNGLNISFQRLIQEKKKNNSELAFSNKGKII
                     KVKAAEL"
     gene            5910..7463
                     /locus_tag="P5293_04400"
     CDS             5910..7463
                     /locus_tag="P5293_04400"
                     /inference="COORDINATES: protein motif:HMM:NF037031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HRR49177.1"
                     /translation="MKFYLLLLSLFSSSFLFSQVRFSSDFESGSMGEVKLIDSVWIKT
                     SPMDSMLTLSYDVYSRLDPLNPIDTTLEPSSRWYYFRITGVKGKQLFINIRNSEVVRP
                     FYSYDNTHFTRLDWSENVNKGAINKIFTRDTVYISYFIPYTCTHLSTKLSDWSKNKFV
                     KEEQIGKSQQGRDIWMMTITDWSVNDQSKKKVWIHGRTHPSETPSSWHLEFFVDKLLD
                     GSKQSNAILANTIFYIVPFNNPDGVYGGFSRSTSTGVNIEINWDKPDSLTMPEVLVLK
                     NKISELTKDGSFDLLLNMHSQIANNITYWVHTAESTSDEMFRKQLLLSHLTINNNPYF
                     CSTDLSFSNLAPKYAEGWMWNKAGNNCVAITFETPYTFYKNQGDDEWVSLENLKDLAS
                     NSLIAISDFLEISSPTRVLADIKVKNNKKWKKIDDNSLIYFGNDCMTTDLKGLKVKAN
                     VNFLEPGLYNIFSWTPGISSKSSVEGTNCWKEIGTVFVTQKGKYLWKGYSEQINGIAD
                     KILFENIFL"
     gene            7496..7568
                     /locus_tag="P5293_04405"
     tRNA            7496..7568
                     /locus_tag="P5293_04405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7529..7531,aa:Gly,seq:ccc)
     gene            7593..8000
                     /locus_tag="P5293_04410"
     CDS             7593..8000
                     /locus_tag="P5293_04410"
                     /inference="COORDINATES: protein motif:HMM:NF039669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6146 family protein"
                     /protein_id="HRR49178.1"
                     /translation="MKTIVRFSFFTLLTLLAISCSSQRNLSANMTGRVAIHDTIPVED
                     TVEYELIVFDPGFESWLISNPHSQHMYSNEYLQNMNYQYVIAWNYLYSRGHPRVESYL
                     DYDPTIKYNYDFNYKLFMYFKYFEETNRIKLIP"
     gene            complement(8028..8348)
                     /locus_tag="P5293_04415"
     CDS             complement(8028..8348)
                     /locus_tag="P5293_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49179.1"
                     /translation="MKKALLLTIVPMLMTTCFHTVSFAQKVVQIGSTEIVLQSPLKVN
                     TAETEMKYRDGRSFNPFNVGLQCSLGFNGFSVYAKYRLTNCFNHYNIELEVPRLNIGI
                     QFEI"
     gene            complement(8353..8841)
                     /locus_tag="P5293_04420"
     CDS             complement(8353..8841)
                     /locus_tag="P5293_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HRR49180.1"
                     /translation="MNKSAILAVLLFIFPSWNLLGQTKQLDKIFSTYENYKNVQSVSI
                     NPAAFFHLSEETKSNELLSKMSLLKILNVTKGGNEKDKNVWINLKSDVEKFIRENNFS
                     QILKVKDKDELLEMYSRKEDNSLLLLTADSQSEYAVFYIQGDIDKSVIDALMKGNIKI
                     KK"
     gene            complement(8870..9379)
                     /locus_tag="P5293_04425"
     CDS             complement(8870..9379)
                     /locus_tag="P5293_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49181.1"
                     /translation="MKKEKVEKLLKLYFDGKSSVREEKLLRHYFLSDNILPEFMKYRD
                     LFNFFSSEAKLINSYRYKENKNKFILSFTAVAASFIAFLLIINNSEADKIRVVVGGEK
                     MKDREIALQIVDKQLSKINIMYDIADRNVNKLNNMSVFEKCFWFVDENGAIRNVPKLL
                     DKLSGEYSE"
     gene            complement(9387..9884)
                     /locus_tag="P5293_04430"
     CDS             complement(9387..9884)
                     /locus_tag="P5293_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR49182.1"
                     /translation="MTQREFEREIVRIRPVLLQFARSFNIVGASCAEDMVQEAVMRVW
                     QSILGGEVVRSMEALTKRILRNVCLDYLRLKKNICNDYDIYGVDKIDSYDTARQLEIK
                     EEFTRIVEIIKTLPVDQQLCIRLKDMMGFENWEIAEILNTTEVNVRTLLSRGRKSIRN
                     ELIMK"
     gene            complement(10018..10254)
                     /locus_tag="P5293_04435"
     CDS             complement(10018..10254)
                     /locus_tag="P5293_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006948103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRR49183.1"
                     /translation="MKQQIIEALNLEEMKPEDIDTDAPLFVDGLGLDSIDALELVILL
                     EREYGIRLEDPKANKEIFKSVRTMAEFIEKNRKM"
BASE COUNT         3362 a         1844 c         1836 g         3213 t
ORIGIN      
        1 ttgttgaatt ttacaaaaat gctggattta ccggaggtgg taaagaattt aaaccactta
       61 aaaaaattaa ctgatataat aaattaaact gaaataatat aattatggaa caaacattgc
      121 ctttaatttt ggcttacacc ggcctggctt tcatgctcac acttgcttgt attggaagtg
      181 ctcttggtgt ttcaatttgt ggtaacagta ctattggtgg tctcaagaaa aaccctgaca
      241 tttttggttc atcaatgatt cttacagctc ttccttcaac tcagggcctt tacggttttg
      301 ctgcattctt tcttgcacta agcaggctta aatcaatttc tgacctcact ctttccgggg
      361 gtcttgctat ccttgcagta ggtatagctc ttggctttgt tggtctgttc tctgcatttt
      421 atcaggcaaa attgtgtgct aatggcatta acgagatgag taatggccag gatgtatttg
      481 gaaaaacact cattcttgca gtattccctg aactttacgc aatccttgcc tttgcagctg
      541 ctttcctcgc tcttccgtaa acgttttaaa tataagtata attcgggtgt caggatatta
      601 aagcttgaca ccctcttttt ttgtacattt gtaaataaaa aaacagacct atgactctaa
      661 ttaaatcaat ttcaggcata agaggtacaa ttggaggaaa agttggagat gcactcacac
      721 ctccggacat tgtgaagttt actgctgcat acgtaaaacg tttaaaagaa agaataccgg
      781 ataaggatag ctacaaaatt gtagtaggaa gagatgcgag gatatctggc gaaatggtag
      841 ataaaattgt atgtggaact cttatggcct gtggcattga tgttgtcaat gcaggacttg
      901 cttctactcc aacagttgag attgcggtag ttttcgaagg tgcagatgga ggaataattc
      961 ttactgcatc acataatcca aaacagtgga atgctttgaa acttctaaat gaaaacggcg
     1021 agtttctcag tagtgccgag ggcgaattac ttcttaaaca tgctgaagag gaagcatttg
     1081 attttgtatc ggttgaacga cttggaaaat gcacatgtaa agattttacg tccaaacatg
     1141 ttgaagcagt cctgtcctat cctttagtgg atatagaatc tatttacaga aatggattta
     1201 aagtcatact cgatgctgta aattctgtcg gaggtataat catgcccaaa cttctcgaag
     1261 caatgggtgt tacatgtatt aaaatcaact gtgagcctac aggtaatttt gctcataacc
     1321 cagaacctct tccagaacat cttaaagagc tttccacaaa agtcgttgag gaaggcgcag
     1381 acctgggaat atcggtagat cctgatgtcg acagacttgc gctcatctgt gaagatggta
     1441 ctccttttgg agaagaatac acactcgttg ctgcagctga ttatattctc gaaaaaagaa
     1501 aaggtgctac cgtttcaaac ctttcctcat caagagcatt aaaagacatt actgaagttg
     1561 caggatgtgt ctattccgca gctgccgtag gagagattaa tgttgtaaac gaaatgaagc
     1621 gtgtaaatgc tgtcataggt ggtgaaggaa acggaggggt catcgtccca gatttccatt
     1681 acggaagaga tgccttaatt ggcactgcac ttattcttag ccatatagcc aataagaaga
     1741 ttaaagcttc atctcttaga gacagctatc caaaatatta tatttcgaaa aacaaaatag
     1801 aactttctga taaaatagat gtcggatatc ttttcgaaga gattaagaaa gtattctcta
     1861 ataacaaaat aaccgatatt gatggtgtca aaatagattt tgacaaagag aaaaaatggg
     1921 ttcatctccg taaatccaat acagaaccaa taatacgaat ttacgcagaa gctccgacaa
     1981 ttgaagaagc agattcactg gcaaaattga taattgacaa aatacaaaat attataaaat
     2041 aacttttaat gttttcattt aggacaactc cctttctcga ccaacctgac atcgtattaa
     2101 gaagtggcag gcttggtgtg ttatgcaatc aaactgcatg gcttcctgaa aaaggagaat
     2161 atctgtttga gacacttttt aaaagtggtc gtctgaaaag aatattcaca ccggaacacg
     2221 gtcttttcgg tgaacttcaa gatcaggaga gactggacag tactgattca tacaaagcgc
     2281 ttggcctgaa cggatgtgaa ataatctcac tatatggctc agatgaaaaa tctctgactg
     2341 taagcgcctc caagcttaat gatattgatg cattaattgt tgaattgcag gatatcggat
     2401 cacgacatta tacatatact tctacccttt ataacctgtt caaagttttg aagcaggaag
     2461 agataaactt gcctatatat attgtagatc gtctaaatcc tgccggtaga caagttgagg
     2521 gaacaatgct tgaaaaaaat tacgaatctt ttataggtat tgaaggaata gttcacagac
     2581 atggtctaac ctttggtgag ctcgcaaata tgttttacaa tgaacttaat gccaagtttc
     2641 cactccatat tatctcatat aaagcagaaa gagcagtgaa ggaacttctt ccatggagta
     2701 ttcctccttc tccaaatatt cccggtcttt ttacctgtca tttttacagt ggtcaggttc
     2761 tatgggaagg aactaatgtc agtgaaggaa gaggtaccac aagaccattt gaacaatttg
     2821 gagcaccctt tatgttaaat ttgctgtcat ataaccaatt acatggatta aaaaactgga
     2881 atgaccctga taatcccata tttgattccg ctgtttacat cagatggcaa aaatttattc
     2941 ccacatttca caaatataag ggagagattt gctcaggctt tcaattaata cctgttccag
     3001 gaatgcaata tcatgcattg gcacacaatt tgaaaatcat aagatttgta gcagaaaact
     3061 gttcagaatt tcaatttcgt gaaggcattt atgaaagagg aaacgataaa cgtgcaatag
     3121 agcttctgtt gggagacaag ttgcttttgg attacgttag tggtaatgtt gactggaatc
     3181 aagttaagga tcacatcaag atcgaagagc aaaaatggat aagaaaagca aggaagtttc
     3241 tgctttatga tgaacagtta tttaggtgta aacaatttta aaaaattaat aaatatggca
     3301 aatccagttt taactgagaa ggtttttgag aaaagtagag ttaccgcagg aacaggtgca
     3361 atgactatta aaggtactgc ctcaaaatca cttgtaatga ttttgatgat tcttgccggt
     3421 gcaatgtata cctggaaagt tgcttttcaa gccataaatc ccgcttcagt ccaaccatgg
     3481 atgtggggag gtgttattgg aggctttgta gttgcaatga ttattagttt caagccaatt
     3541 tgggcacagt atcttgctcc tgtttatgct gtacttgaag gacttttcct tggtggaatc
     3601 tccgctctgt tcagtcaggc atttgcagca actgcccctg gaatagtaat gaatgcaact
     3661 ttacttacaa ttctaactgc cgtatcaatg ttgcttgtct acaggacaaa cataataaag
     3721 gtaaatggca aattcgcaag aataatgata attgcagttg gagctattgc actttattac
     3781 ctggtttcta taattgtatc actttttgga gttgatctta cattgctcaa tgacagtggc
     3841 cctttaagca ttggaatcag ccttgtcata attggtgtag cagccttcag cctgctgctc
     3901 gactatgagt ttatagcaag agcatctgaa gcaggtgctc caaaatacat ggagtggtat
     3961 ggagcattta gtttaatggt aacattgatt tggctttatc ttgaaatctt acgaattctt
     4021 gcaaaatttg caatagggcg tgaatagttt tggctattga taaattattt gtacatttgc
     4081 agccccataa tatttaacag cataagaaat gaaaaaagga atacatcccg aaaactaccg
     4141 tcttgtagct ttcaaggata tgtctaatga ctacgttttt attactcgtt catgtgcaaa
     4201 cacaagagag acaattgttc atgacggagt agaataccct cttgtaaaag ttgagatatc
     4261 caatacttct catccattct atacaggcaa gatgaaactt gtagatacag ccggtcgtgt
     4321 cgataaattc ctaaacagat acaagaatat aaagaaataa gtttgctaaa taataaacta
     4381 aaaaggccct taagggtctt tttagtttat tatttgttaa ctttggaaaa agagtttaat
     4441 ggaaagcaca tctgaaaaac tgatcaaact ggcagcagag tacaataatc ttaaatattt
     4501 cactactgat cctattattt tccccagata cttttcagaa aaattaattc aaggagaatc
     4561 atcacttcaa gacgttgaaa ttgccgggat cttgtctgcc catcttgcat ggggaaaaag
     4621 agagttaatt gtcagggatt gcaacagaat gtttgatgaa atgaaatggc agccatattc
     4681 ttacataatg gcaggagatt tcaaaagtga ttctgtatct cttcacagaa gtattaaatg
     4741 gagtgatttt gcaaaaattt gtgaaaactt gaggcagttt tatcttaaca acgaatcact
     4801 ggaatgtttt tcaccctctt acataagact aaatatttat ggatgcaggg acaatcctaa
     4861 tgctgccaat aaaaaaattc acatgtttcg cagatggatg gtcagaaatg acggtatagt
     4921 cgatctggga ctctggagaa aaatttcgcc tgcagatctt ataatacctc ttgacactca
     4981 cgtccacaga agttccatta acctcggcat aaccaaaaga aatgtatctg actataagac
     5041 agccgttgag ataactgaat atctcagaaa agttttcccg gatgatccct gccttggaga
     5101 cttttcactt tttgctttta ctgcaacaat cggtaaaaaa aatggttatt tttgatttgg
     5161 cttatgaaaa atttatttat catagcaggt tgcaacggat caggaaaaac tacagcttct
     5221 tataatatcc ttccagaaat gctcggctgt aaagaatatg ttaatgcaga tgaaatagca
     5281 agaggaatat ctccctttaa accggaaagt gtttcaatcc aggccggtaa gataatgctt
     5341 gcaagaataa aagaactcat agccaacggc acggattttg cgcttgaaac aacgttagca
     5401 acaagaactc atgcaaactt tatcagatat gcacaggagc atggctataa ggttacactg
     5461 gtttttttct ggctaaactt gccaaacctt gctgttgaga gagttaagat gagggttgaa
     5521 agcggtggac ataatattga tgaaaaagtt ataagaagaa gatatgattt tggattaaaa
     5581 aacttatttt cgctttatgt tcctttatgc gaatattgga tgataattaa taactctact
     5641 ttgccacctg agctgattgc agaaggaggg aagaaccagg agacaaaggt atacaataaa
     5701 ccaacctaca ataaaatagt taattatgag cgaacaagag actaaagagc ttagggataa
     5761 tatcctgaac ggtctgaaca tctcatttca gcgattaatt caggagaaga agaagaataa
     5821 ttctgaactt gccttttcta ataagggtaa gattattaaa gtaaaggccg cagaacttta
     5881 actctaatat ctgcttagat atgaccggaa tgaaatttta tttactgctg ctgagtttgt
     5941 tcagtagtag ttttttattt tcacaagttc gtttttcctc tgattttgaa tctggaagta
     6001 tgggggaagt aaaacttatt gattccgttt ggatcaagac atctccaatg gactcaatgc
     6061 tgactctttc ttatgacgta tattcaagat tagatccttt aaacccaata gacaccaccc
     6121 ttgaaccaag ttcgagatgg tactatttca gaatcacagg cgttaaaggc aagcaactct
     6181 tcattaacat aagaaattcc gaagtagtaa gaccatttta ttcctatgac aacacccatt
     6241 ttacaagatt agactggtct gagaatgtta acaaaggagc aattaacaaa atttttacaa
     6301 gagacactgt ctatatttct tatttcattc catatacctg tacgcatctt tcgacaaaac
     6361 ttagtgattg gagcaaaaat aaatttgtaa aagaagaaca gattggaaaa agtcagcaag
     6421 gcagagatat atggatgatg acaattactg actggtcagt aaatgaccaa tccaaaaaaa
     6481 aggtatggat tcatggaaga acacatccaa gcgaaacacc atcaagctgg caccttgaat
     6541 tttttgttga taaactattg gatggttcca aacaatcaaa tgccatactt gcaaatacaa
     6601 tattttatat agtaccattt aataacccgg atggagtata tggtggtttc tcaagatcaa
     6661 cctcaaccgg agtaaatatt gaaattaact gggacaaacc tgattcactt actatgcctg
     6721 aagttttagt gcttaaaaat aagataagtg aattgacgaa ggatggatca tttgatcttc
     6781 tgctcaatat gcactcacaa atagccaata acataacata ttgggttcat accgcagaat
     6841 caacctcaga tgagatgttc cgtaaacagt tgctcctatc ccaccttact attaacaata
     6901 atccatactt ctgttctacc gacctctctt tttccaatct tgctcccaaa tatgcagagg
     6961 gatggatgtg gaacaaggca ggtaataact gcgttgctat tacttttgag acaccttata
     7021 cattctataa aaatcaaggt gacgatgaat gggtctccct cgaaaatctc aaagatcttg
     7081 catcaaactc tcttattgcc ataagtgatt ttctcgaaat tagttcgcct acaagagttc
     7141 ttgcagatat taaagttaag aacaacaaaa aatggaaaaa aattgatgat aattcactta
     7201 tatattttgg aaatgattgc atgacgaccg atttaaaagg attgaaagtt aaagcaaacg
     7261 tcaatttttt agaaccaggc ttgtacaata ttttctcatg gacaccggga atttcttcaa
     7321 aaagctctgt cgaaggaaca aattgctgga aagagatagg aactgtattt gtgacacaaa
     7381 aaggaaaata cttgtggaaa ggctattcag aacaaattaa tggcattgct gacaaaatcc
     7441 tatttgaaaa tatttttttg taaatttgta gatgtaatgt gctattgatt caaatgcgga
     7501 agtagctcag taggcagagc atcagcttcc caagctgagg gtcgcgggtt cgaatcccgt
     7561 tttccgctca aaagttcata aatttttgaa agatgaaaac aattgtcaga ttctcatttt
     7621 ttaccttgct gacactgttg gccatctcat gcagttcaca gagaaatttg tcagccaata
     7681 tgacaggaag ggtagcaatt cacgatacca ttcctgttga agacactgtt gaatatgaat
     7741 taattgtatt tgatcctggc tttgaatcat ggttaatcag taatcctcac agtcaacaca
     7801 tgtactctaa tgaataccta caaaacatga attatcaata tgttattgca tggaactatc
     7861 tttattcaag aggacaccca agggtagaga gttatctcga ttacgatcct acaattaaat
     7921 acaactacga tttcaactac aagctcttca tgtattttaa atattttgaa gagactaaca
     7981 gaataaaact gataccttaa aaaatcaatt tctgtactgg ttacaggtta tatttcgaac
     8041 tggatgccta tatttaatct aggcacttcc agttctatgt tatagtggtt gaagcaattt
     8101 gtgagtctat actttgcata tacagaaaag ccattaaatc ccagagaaca ctgaagaccg
     8161 acgttaaaag gattaaagct tcttccatct ctatatttca tctcggtttc agccgtatta
     8221 accttgagag gtgattgtaa gactatctct gttgatccta tttgaactac tttttgagca
     8281 aatgatactg tatggaaaca tgttgtcata agcatgggca caatagtcaa taatagagcc
     8341 ttcttcatgt tcttattttt ttattttaat atttcctttc attaatgcat ctatcacaga
     8401 tttgtcaata tccccctgaa tataaaatac agcatattct gattgagaat cagcagtcaa
     8461 caaaagcaaa gaattatctt cttttcttga atacatctct aacaattcat ctttatcctt
     8521 taccttaagg atttgtgaga aattattttc acggatgaat ttctcgacat cacttttcaa
     8581 attaatccag acattcttgt ctttctcatt tcctcccttt gtgacattta atattttaag
     8641 taacgacatt ttactaagaa gttcattgga ttttgtctct tcagaaagat gaaaaaatgc
     8701 ggctggattg atagataccg attgaacatt cttatagttc tcataagttg agaaaatttt
     8761 atctaattgt ttcgtttggc ctaaaagatt ccaagatgga aagataaata atagaactgc
     8821 caatatcgcc gatttattca tattaactat tgttaaaatt cataataatt tattcactgt
     8881 attctcctga caacttgtcg aggagtttgg gcacatttct aattgcacca ttttcatcaa
     8941 caaaccaaaa acacttttca aaaacagaca tgttgttgag tttattgaca ttcctgtctg
     9001 caatgtcata catgatgtta atctttgaaa gctgtttatc cacgatttgt agggctatct
     9061 ctctgtcttt catcttttca ccaccaacaa caacccttat tttatctgcc tcagaattat
     9121 ttataattaa taaaaaggca ataaaagatg ctgcaactgc agtgaaagaa agaataaatt
     9181 tatttttatt ctccttgtat ctataactat ttatcagctt tgcctcagat gaaaaaaagt
     9241 taaacaagtc tctatatttc ataaattcag gaagaatatt atcagacaga aaatagtgtc
     9301 taagtaattt ttcctcacgc actgagctct ttccatcaaa atagagctta agtaactttt
     9361 caactttctc tttcttcatt ttatcttcat ttcattatca gttcatttct tattgacttg
     9421 cgtcctcgtg agagcaaagt tcgtacatta acctcagtag tattgagaat ctctgcaatt
     9481 tcccaatttt caaatcccat catatctttc aaccttatgc acaattgctg atctacagga
     9541 agagttttta taatttctac tattcgtgta aattcctctt ttatctcaag ttgacgagcc
     9601 gtatcataac tatctatctt atcaacacca taaatatcat aatcattgca gatattcttc
     9661 ttaagtctaa gataatcaag acacacattt cttaagatgc gttttgtaag tgcttccata
     9721 ctcctcacaa cctctccacc taaaatactt tgccatactc tcatgactgc ctcctgcacc
     9781 atatcttctg cacaagacgc ccctacaatg ttgaacgagc gtgcaaactg caataaaaca
     9841 ggtcttatcc tgacaatctc cctttcaaac tcccgttgag tcattatcaa atattcaaca
     9901 ttaagacgaa ctatttgcga tattgttgca aatagacatt actaactgca acatgatcga
     9961 attgcagaca gataaacttg acaataaaag atatctaact tactttaaag cagaaattta
    10021 catcttcctg ttcttttcta taaattctgc catagttctg acagatttaa aaatttcctt
    10081 atttgctttc ggatcttcca gacgtattcc gtactctctt tcaagtaaaa ttaccaactc
    10141 gagagcatca atagagtcaa gcccaagacc atcaacaaac aagggagcat ctgtatcgat
    10201 atcttcaggc ttcatctctt caagattaag cgcctctatg atttgttgtt tcagt
//
LOCUS       DAOTQL010000053        10010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6000_length_10010_cov_4.584430, whole genome shotgun sequence.
ACCESSION   DAOTQL010000053 DAOTQL010000000
VERSION     DAOTQL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_10010_cov_4.584430"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="miaB"
                     /locus_tag="P5293_04440"
     CDS             <1..1158
                     /gene="miaB"
                     /locus_tag="P5293_04440"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRR49184.1"
                     /translation="WGRLEHFKTMKKKKSSLTVGILGCMAERLKEELLNHPAVDLVAG
                     PDSYRDLPNLLVQIREGSKQINTILSQEETYADITPVRMDPNGVSAFISIMRGCNNMC
                     TYCVVPYVRGGERSRDSESIVREATELYENGYKEVTLLGQNVDSYQWVDKDNPLKTTN
                     FAQLLEKVALISPKLRVRFSTSHPKDMSNSVLYTMAMYDNICKHIHLPVQSGSDSVLS
                     RMNRKYTRNEYLERVAMIHKIIPDCSISTDIIAGFCGESEEDHEHTMSLMRDVEFYTA
                     FMFQYSERPNTKAARRYKDDVPSNVKNRRLNEIIKLQNELSLASNQKDLGKVFEVLVE
                     GTSKKSKSELCGRNQQNKMCVFPADGHKTGDFVNVRITSCTSATLKGEVVK"
     gene            1178..1588
                     /locus_tag="P5293_04445"
     CDS             1178..1588
                     /locus_tag="P5293_04445"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRR49185.1"
                     /translation="MTKFKTTYIGNLQTEVIHLKSGSKIITEAPEDNNGKGTMFSPTD
                     MFAASLASCMMTIMAISAQAHGFSIDGMYIETEKIMAANPRRVGELKLDIYLPKKDYT
                     EREMRFIETSVKTCPVANSIHPDIVKTITYHLPE"
     gene            complement(1608..2693)
                     /locus_tag="P5293_04450"
     CDS             complement(1608..2693)
                     /locus_tag="P5293_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49186.1"
                     /translation="MKKKCIKWLFAALAVIFLNVSCSKDDNPELIGNKGKVLVLNQGN
                     YTEQSASISLYDETTNQITNRLYESANGVSIGATLNSGYITPYGDAALICNYPDKILF
                     INPETGVDKGYSITEGLVSPRNMVMSDNYIYVTNWGSDHIVLPSGSWEFDKSYISIYS
                     FSTLAFQGNIDVGSDAEGIILYDGRLYVAVKQGLKIFDIVGNSLRLAATLKPTTFVNG
                     ARHVVVDGMNKIWVSFPGDGLMRINPSSLEIMSTLDVPVDAMDGYIAIDKNGDYIYTY
                     CTTFDSNYSPVEANIYKVSVKSNSYSKLFTGTYFYGVGVSPVTGNIFTSEVSFTSNSV
                     LKVVAPSDGSIRNSATAGIGTCRYLFF"
     gene            complement(2703..4637)
                     /locus_tag="P5293_04455"
     CDS             complement(2703..4637)
                     /locus_tag="P5293_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49187.1"
                     /translation="MQTRFVRLSLFFILFSSICSVAEAECRFITNDTIKKRRLDSIKI
                     VSQKTSGFSSNSRFSVGTNFIKFDDGTLAPVRANSLSDFIQKENASYIKEFGRGMNAY
                     ISIRGSSSSHTTVDWNGMNLAMPTLGQTDFSHIPLYLFDVVELHIGGNSAIYGAGAIG
                     GTVQLKTSPKWEKGVHGDILVSQGSFNSLFTGGTLRYSNSSFETRTSLLYSFAENNYE
                     FINNTKLGKPKERLNNSSSKNAGLLQEIFRKFRDSSMLSATLLYLDFDRDIQPSVSLN
                     DRPDTYRSIYDNNLKMSLGYSGAKGIVTYAAHLSYSFDKQLYEDDRIAAGRFSFSTDI
                     ESKFGDMVVKGGFFAERTTPQVDSYADSVKENRLNIYLLGSYNFNNRLMLAGGVRFAC
                     ATDVSVPLMPSLDVRYNVLNNNSHSLAIRCALSGNSKIPTLNDRYWGGEHLYLKSESS
                     ITLEGGFNYNWFSSVWSLDMFGTLYKSKVNDWIRWLPAGTVWRPQNIPLVVTRGGEAG
                     LKIGRSFSTIKTFFNFSLAYTNIKMVEGLWDEDPAIGEQLAYQPKLSWRSGLRVVKDN
                     WEIYSNVSYTGKRTTVDLYDILPSYTLLDIGTSCNLRLFGCNFVLNGIIKNITNEMYQ
                     NVKFYAMPGRNYQLSLQYKF"
     regulatory      complement(4664..4874)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4950..5033
                     /locus_tag="P5293_04460"
     tRNA            4950..5033
                     /locus_tag="P5293_04460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4984..4986,aa:Leu,seq:caa)
     gene            complement(5250..6614)
                     /locus_tag="P5293_04465"
     CDS             complement(5250..6614)
                     /locus_tag="P5293_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR49188.1"
                     /translation="MKFVNRTEETKRLKQVLSADKSTFTVVYGRRRLGKSTLITRVLN
                     DNDVYYLADQTEASLQREVLAKMIAVIIPGFDKVIYPSWNVFFDTLNDRTTQMFTLCI
                     DEFPYLVRSSPDLSSVLQNKLDSKKLKFNLVICGSSQQLMYGLVLDASSPLYGRADVI
                     LKIAPMKVPYIQEALGVSSLDAVEEYAVWGGVPRYWELREGYANLREAIISNMLSVNG
                     TLYEEPVKLFKDDINDIVKTATIMSYVGAGANRMSEIAGRSGEVVTNLSRPLAKLISL
                     GYLTKEIPFGENEKNSKKSLYKISDPFMNFYFQFVIPNRSFIEIGRIAPILSNLDSHF
                     SDYVGDYWEKLCRETVSGNEIDGVTYGIARRWWGNVSRDERVELDIVAESLDKKYLLV
                     GECKWTTKENSDRILSELIEKANKLPFARNHTLVPKLFLKNKPVGNLDNVLLPDDIMK
                     LYID"
     gene            6775..7215
                     /locus_tag="P5293_04470"
     CDS             6775..7215
                     /locus_tag="P5293_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49189.1"
                     /translation="MKRLIINAMAVIKKGVGILLWLLVSAFPGFSQEHRGYSIEVRTD
                     KNGTLNFYCENERHITYTIRVDLYNYINYAPSCTFPFAGEAKAGTSFLFKLTPILPSA
                     SPTYRMVTDFKLGKYIENPDSNYLYLLPLKAQREIIVFKNIFTD"
     gene            complement(7421..7543)
                     /locus_tag="P5293_04475"
     CDS             complement(7421..7543)
                     /locus_tag="P5293_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49190.1"
                     /translation="MPKVKRKNARQLEKFLPAIIETVNSITIVANSRNHSIGTV"
     regulatory      7942..8126
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8189..>8535
                     /locus_tag="P5293_04480"
     CDS             8189..>8535
                     /locus_tag="P5293_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49191.1"
                     /translation="MRKKYFTKLTLAVLLLLPFSFVATSCLDDDSEFEEQVRIDDQKI
                     NKYLTDNNIVAQKHNSGIYYRKLTENNTGTSLNRGNVVSFYYSISLLDGTVLQSISKE
                     NGDKPLQFCLLSNT"
     assembly_gap    8536..8635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8636..8968
                     /locus_tag="P5293_04485"
     CDS             <8636..8968
                     /locus_tag="P5293_04485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRR49192.1"
                     /translation="PGTGFRPFVGSRVDIDFTRKYLNDSIITSATGVSFFLDHFQAVQ
                     GLEEGIKLMKEGGYAILLMPAKLGFNESLCIIPQKVRKELLYDRVITSEVEPYSILKY
                     VVRLRNTN"
     gene            9017..9574
                     /locus_tag="P5293_04490"
     CDS             9017..9574
                     /locus_tag="P5293_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR49193.1"
                     /translation="MVKQEEQDITVIIQGCIRQNEKSREVLYRKFFGYAMSVALLYAK
                     ERNDAIEIVDDSFIKVFHEIKRYDTSKPFKIWLRKIVINTAIDKYRKELKRGVMVDVQ
                     ELTLQDSHQDVVGSITFKEIINMLKYLPQLHRTVFCLYEIEGYSHEEISKELRIPESS
                     SRVYLSRSKKRLQEIFKLHFNTGHK"
     gene            9591..>10010
                     /locus_tag="P5293_04495"
     CDS             9591..>10010
                     /locus_tag="P5293_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49194.1"
                     /translation="MATEENEIVEIIKKTALEHEEIYEEGAWENFVLKQRRKRRALFL
                     KISSGAAACMIIGLALFLTFKQPTVQNNNNATIVLNDKKQTKTPIIKQNFLIKTPDLF
                     KEDLTIKKIIPKEEIAANRIDRSKIDNGQNLIEVSTVK"
BASE COUNT         3174 a         1929 c         1801 g         3006 t
ORIGIN      
        1 tggggaagac ttgaacactt caaaacaatg aagaaaaaaa aatcaagcct gactgtcggg
       61 atattgggat gtatggcaga aagactgaaa gaggaattac taaatcatcc tgcagttgat
      121 ctggtggctg gacctgattc atatcgagat ctccccaacc tgcttgtcca aataagagaa
      181 ggcagcaagc agataaatac aatcctttct caggaagaga cctatgcaga cataactcct
      241 gtgagaatgg acccaaatgg tgtaagtgca tttatctcaa taatgagagg atgtaacaat
      301 atgtgtacct attgtgtagt cccctacgtg agaggaggag agagaagcag ggattctgag
      361 agtattgtcc gtgaggccac cgaactatac gaaaatggat ataaggaggt aactcttctt
      421 gggcagaatg tcgattcata ccaatgggtt gacaaagata atcctttaaa aaccaccaat
      481 tttgcacaac ttctcgaaaa agtcgctcta ataagcccaa aattaagagt acgcttttct
      541 acttcacatc ccaaggatat gagtaattct gtcttataca ccatggctat gtacgataat
      601 atttgtaagc acattcacct ccctgtccag tccgggagcg actctgttct ttcaaggatg
      661 aataggaagt ataccagaaa tgaatatctt gagagagtag cgatgatcca caagataata
      721 ccagactgct ccatcagcac cgatattatt gccggcttct gtggcgaaag cgaggaagat
      781 catgagcata ccatgtcatt aatgagagat gtggaatttt ataccgcttt tatgtttcaa
      841 tactccgaaa gaccaaacac taaggcagcc aggcgttata aagatgatgt tccttccaat
      901 gtaaaaaaca ggagactgaa tgagataata aaacttcaaa atgaattgtc actcgcaagc
      961 aatcagaaag acttaggcaa agtattcgaa gttttagtcg aaggaacttc aaaaaagtcc
     1021 aaatcagaac tgtgtggcag aaaccaacag aataaaatgt gcgtattccc tgcagacgga
     1081 cataaaacag gagattttgt caatgtccgg ataacatcct gcacctctgc cactcttaaa
     1141 ggagaagttg taaaataatt tttttaaacg ataagatatg accaaattta agacaaccta
     1201 cattggtaac ctccaaacag aggttattca tctgaaatct gggagcaaaa ttattactga
     1261 agctcctgaa gacaataatg gcaagggaac tatgttttcg cctactgata tgtttgctgc
     1321 ctctcttgcc tcatgtatga tgacaattat ggcaatctca gcacaggctc atggtttttc
     1381 aatagatggg atgtacattg aaacagagaa aataatggct gctaatccca gaagagttgg
     1441 agagctgaaa cttgatatct acctcccgaa gaaagattat acagagaggg aaatgagatt
     1501 tatcgaaaca tctgtcaaaa catgccctgt agcaaacagc atacaccctg acattgttaa
     1561 gaccatcaca tatcacctcc ctgaataaag ttaatcttta aactttttta aaagaaaagg
     1621 tagcgacaag ttccaatgcc ggcagttgct gagtttctga tcgaaccatc agaaggtgcc
     1681 actaccttta aaactgaatt tgaagtaaaa cttacttctg aagtgaaaat attgcctgtt
     1741 acaggactaa ccccaacccc gtaaaaataa gtgcctgtaa ataatttgct gtaactgttg
     1801 ctttttacac ttaccttata aatattggcc tctacaggag agtagttgga atcgaaagtt
     1861 gtacaatatg tataaatata atccccgttc ttatcaatgg ctatgtatcc atccatggca
     1921 tctacaggaa catcgagagt tgacattatc tcaagtgacg aagggttgat tctcatgaga
     1981 ccgtctccag gaaaagagac ccaaatttta ttcatgccat ctacaacaac atgtcttgca
     2041 ccatttacaa aagttgttgg cttgagtgtt gccgccagtc tgagactgtt tccaacgatg
     2101 tcaaaaatct taagtccctg ttttactgcc acataaagtc ttccgtcata taatattatc
     2161 ccctcggcat ctgaaccgac atctatgttg ccctggaatg ctaatgttga aaaagaataa
     2221 atagatatat atgatttgtc aaactcccat gaacctgatg gaagaactat atgatcagac
     2281 ccccagtttg tcacataaat atagttgtca gacattacca tgttacgagg agacacaagt
     2341 ccctcagtaa tactgtagcc tttatctaca cctgtttcgg gatttataaa caaaatcttg
     2401 tccggatagt tacatataag cgcagcatct ccatagggtg ttatatagcc actgttaaga
     2461 gttgctccta tcgaaacccc gttggctgat tcataaagac gatttgtaat ctgattagtg
     2521 gtttcatcat aaagagatat acttgcagac tgctctgtat aattaccctg atttagaacg
     2581 agtaccttac ctttgttacc aatcaattca ggattgtcat cctttgaaca tgacacattt
     2641 agaaaaataa ctgctaaagc agcaaataac catttaatac attttttttt cataactata
     2701 ttttaaaatt tatactgtag tgacaattga taatttctcc ccggcattgc atagaattta
     2761 acattctgat acatctcatt cgtaatgttc ttgataatgc cattcaaaac aaaattacac
     2821 ccgaaaagtc ttaaattgca ggacgttcct atatctaaca gtgtataaga aggaagtatg
     2881 tcataaagat ccacagtggt ccttttgcct gtataactga cgtttgaata tatttcccaa
     2941 ttatctttga ctactctcag cccgcttctc caagacaatt ttggctgata agcaagttgc
     3001 tctcctattg ccggatcttc atcccacaat ccttcaacca ttttaatatt cgtataggca
     3061 agagaaaaat taaaaaaagt cttaattgtt gaaaatgatc tgccaatttt aaggccggcc
     3121 tctccacccc ttgtcacaac aaggggtata ttttgaggac gccagactgt tcctgccggg
     3181 agccatctga tccagtcgtt cactttgctt ttgtatagag tcccaaacat atccaaactc
     3241 cagacagatg aaaaccagtt gtaattgaaa cctccttcaa gagttatgga actttcactt
     3301 ttaagataga gatgctctcc tccccaatat cggtcattca aggtaggaat cttgctattc
     3361 cctgacaaag cgcatctaat tgctaaagaa tggctgttat tattcaatac attatatctc
     3421 acatcgagcg acggcattaa gggtacggag acatctgtag cacaggcaaa tctcacccct
     3481 ccggcaagca ttaatctgtt attaaaatta taactgccaa gtaaatatat atttaacctg
     3541 ttctctttga cagaatcggc atatgagtca acctgaggtg tggtgcgttc tgcaaaaaat
     3601 ccacctttta ctaccatatc accaaattta ctttctatat ctgttgagaa cgaaaagcga
     3661 cctgcagcta ttctgtcatc ttcatagagc tgtttatcaa atgaatagga aagatgagcg
     3721 gcgtaagtta ctattccctt tgcgccagaa tatccgaggc tcatcttcaa attattgtca
     3781 tatatcgatc tgtaagtatc tggacgatca ttcaaagaca ctgatggttg tatgtccctg
     3841 tcaaaatcga ggtagagcaa tgttgccgat aacatggatg agtctctaaa tttcctgaaa
     3901 atttcttgta acagacctgc gtttttggac gaagagttgt ttagacgctc ctttggcttc
     3961 cctaacttcg tattgttaat aaattcataa ttattctccg caaaagaata gagtaaggat
     4021 gttcgagttt caaaacttga gtttgaatat cttaaagtac ctccagtaaa caatgaatta
     4081 aagctacctt gagagacaag aatatctcca tgtactccct tttcccactt tggagatgtt
     4141 tttaattgaa cagtgcctcc aatagctccc gcaccataaa tcgcagaatt accaccaatg
     4201 tgcagctcta caacatcaaa cagataaagt gggatatgtg aaaagtcagt ctggcccaaa
     4261 gtaggcatag caagattcat cccgttccag tcaacagtcg tgtgagatga agatgatcct
     4321 cgaatggaaa tataagcatt catacccctt ccaaactcct taatatagga ggcgttctcc
     4381 ttttgaataa agtcagaaag agaattcgcc ctaacagggg caagtgtccc gtcatcgaat
     4441 tttataaaat tcgtccctac cgaaaagcga gaattggaag aaaagccaga tgttttttgg
     4501 ctgacaatct taattgaatc taaacgtctt tttttaatcg tgtcattagt aataaatcta
     4561 cattcagcct cagcaacaga acatatgcta ctaaaaagaa taaaaaaaag tgataaacgg
     4621 acaaatctgg tttgcatcgc tctctatctc ccgaaagttc gattaataaa tttcgcccct
     4681 cagcaggttt tctgacttag cttttaaatc tcttgccttc ccaccaaatg gcagtggctt
     4741 gcgaaagata accccttgca gggtaagcat tacagcagcg ggtactgtcc tcgaatctca
     4801 cgaggttccc tttctgttga gctttgcaaa tgtatggatt tattttttta gcaacaaata
     4861 aaccttccag ataactccta tttggctgaa caaatttttt ccttaccttt gtttccctgt
     4921 ttacagcact gttaccgaaa tataattaag cccggatggc ggaatcggta gacgcgctgg
     4981 tctcaaacac cagtggccgc aaggctgtgc cggttcgacc ccggctccgg gtacggagag
     5041 aaataaactc taattgataa tgagtcagtt agggttttct cttttacaat ttgcgccatt
     5101 tttgcgccat atttgaaaaa ataccgatga aattaaactg aaaattatct ctttgtaatc
     5161 agttatccct cgtttgaaaa caaatgattt tgtagaatga aactatgtcc gattatttgg
     5221 gggtgttgga acccctttat ttcagacaat taatcaatat acagtttcat aatatcatct
     5281 ggcagcaata cgttatccag gtttccaaca ggtttgtttt tgaggaaaag tttaggtact
     5341 aaagtgtgat tcctggcaaa tggcagctta tttgcctttt caattaactc ggatagaatg
     5401 cggtcgctat tctctttggt agtccattta cattcgccca caagtagata ttttttatcc
     5461 aaagattcgg caactatatc taattcaaca cgttcatctc ttgaaacatt gccccaccag
     5521 cgacgtgcaa taccgtaagt aacgccgtca atctcatttc ctgaaacagt ctctctgcat
     5581 agtttttccc aataatcgcc cacataatcc gaaaaatggc tgtcgagatt tgatagtata
     5641 ggagcaatcc gccctatttc aataaatgag cggtttggga taacaaactg gaaatagaaa
     5701 ttcataaatg ggtcacttat cttgtagagg ctctttttag aattcttttc gttttctcca
     5761 aatgggatct ctttggtcaa gtatcctaat gatattaatt tggcaagtgg tctcgaaaga
     5821 tttgttacaa cttcgccact tcttcctgct atctcagaca tacgatttgc cccggcacca
     5881 acatacgaca ttatcgttgc cgtcttgaca atatcattaa tatcatcttt gaataatttt
     5941 acaggctctt cgtataaagt tccgtttact gatagcatat tgcttataat tgcctcccgc
     6001 agattggcat aaccctcacg cagctcccaa tagcgtggta ctcctcccca gactgcgtac
     6061 tcctctactg cgtcaagtga agatacgccg agggcttcct gaatgtaagg cactttcatt
     6121 ggagcaattt ttaatataac atcggcacga ccatatagtg gagatgaggc atctaaaacc
     6181 aaaccataca tcaactgttg ggaagagccg caaataacta aattaaactt cagcttttta
     6241 gaatctaatt tattttgaag aaccgatgat aaatcggggc tacttctcac aagatatgga
     6301 aattcatcta tgcatagcgt aaacatttgt gtagtgcggt cgttgagcgt gtcaaaaaaa
     6361 acattccacg aagggtatat taccttatca aatcctggaa tgataacagc tatcatcttt
     6421 gctaacacct ctcgctgtaa agaggcttcc gtttgatctg ctaaataata gacatcgtta
     6481 tcatttagaa ctctggtaat caatgttgac ttacctaaac gcctgcgacc atagaccact
     6541 gtaaatgtag atttatctgc acttaacacc tgtttgagcc ttttagtctc ttctgttcta
     6601 ttaacgaatt tcatatctgt ctatttatta tgcattacaa acataatgtt tgtttagcat
     6661 aattccaaat acttttccgg aattgttatt tgtgcctact tttttgcttt ttcaaacatc
     6721 ttggttgcaa atttttgtct ttttgcaaat tttcacataa atcatttatt atctttgaaa
     6781 agactaataa ttaatgcaat ggctgtaatt aaaaagggag ttggtatact actttggctt
     6841 ttggtttcgg cgtttccggg attttctcag gaacacagag gttactcaat cgaggtcagg
     6901 accgataaaa acggaaccct gaacttttat tgtgaaaatg aaagacatat cacttatact
     6961 attcgggtag atttgtacaa ttatataaat tatgctccga gctgtacatt tccatttgct
     7021 ggtgaggcaa aagccggtac atctttccta ttcaaactga cgccaatcct tccatctgca
     7081 agcccgacat acagaatggt aaccgacttc aaattaggta aatacattga aaaccctgat
     7141 tccaattatc tatacctttt accattaaaa gcccagagag agataatagt gtttaaaaat
     7201 attttcacgg actaaaaatt ttgtctaatt tggaactgtc ctatttttaa ggaaggctac
     7261 ataaacatat gaaatcttct tttcatagtt acaaaagcac attgatagcc attacaattt
     7321 ttagcataat gatggctgcg cttgaagcta ttgttgtaat ttacctaaga caaatttatt
     7381 accctcagga atttgaacca caaccgactg tctcttttct tcaaactgta ccaatagaat
     7441 ggttacggga atttgctaca atagtaatgc tgttaactgt ttcaatgatt gcaggtaaga
     7501 atttctctag ttgtctggca ttttttcttt ttactttcgg catttgggat attttttact
     7561 atgtctggct ctacattctt gtcggttggc ctgaatcgct gctaacatgg gatcttcttt
     7621 ttttgattcc tgtcccgtgg gtagggcctg tattagctcc tgtaatatgc tccttaacaa
     7681 tgatcttaat cgccctgtat attttaaatc atgtttcaaa aggattacgg ttaagatttg
     7741 gattaaggca atggttgctt attgcattag gagtcattgc tattttgttt acatttatgc
     7801 aagattacac tgccatactg attcaagaat atttactctc cggacttgac ggtcttatgc
     7861 agtccccaca ttttcaacaa acaatcagtc aatttatacc tacacatttt aactggctta
     7921 tttttgcaat aggagagatt ttgatcataa tcggctttta cctctgtaac acatctggtt
     7981 ataaaaggga atccggtgaa agtccgggac tatcctagta gctgtaagtt ccttcataag
     8041 atctgtttac tctatgccac tgactatata gttgggatgg ccagaaccaa taatttctcc
     8101 ggtttggtat gcattttaac acgtggtctc tcacaaatgt agcattttta tatttgctta
     8161 cgtatggtca aataaaatta gaaatattat gaggaaaaag tattttacca aattaactct
     8221 tgcggttcta ttgctgcttc cattctcctt tgttgcaaca tcatgtctcg acgatgattc
     8281 ggaatttgaa gaacaagtta ggattgatga tcagaaaatc aacaagtacc ttacagacaa
     8341 caatattgtt gcacagaaac ataatagtgg tatatattac agaaagttga cagaaaataa
     8401 caccggaaca tcactaaata gaggcaacgt tgtcagtttt tattattcaa tttcactttt
     8461 ggacggaaca gtactgcaat ccatcagtaa agaaaatggt gacaagccat tgcaattttg
     8521 tcttttaagc aacacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctgg
     8641 cacaggtttt agaccttttg tcggctcaag ggttgatatt gattttacaa ggaaatattt
     8701 aaacgattcg ataattacat ctgcaacagg agtatcattc ttccttgacc attttcaggc
     8761 ggtacagggc ctcgaagagg gaataaaact gatgaaagag ggtggttatg caatcttgct
     8821 tatgccggca aaacttggat ttaatgaaag tttatgtatt attcctcaaa aggtcagaaa
     8881 agagcttctg tacgatagag ttataacatc cgaagttgaa ccttactcaa tccttaagta
     8941 tgtagtaaga ttaagaaata ccaactgaat ttgaaaataa atccttataa ttgtcaggca
     9001 tgtaaagttc aaattggtgg taaaacagga agagcaagat ataactgtca taatacaagg
     9061 ctgcattcgt caaaatgaaa aatcccgtga ggtgctgtac aggaaatttt tcggttatgc
     9121 tatgagtgta gccttgttat atgccaaaga gaggaatgat gctatcgaga ttgtcgatga
     9181 tagctttata aaggttttcc atgaaattaa aagatatgat acttcaaagc catttaaaat
     9241 ctggttgaga aagatagtga taaatactgc aattgataaa tatagaaaag aattgaaaag
     9301 aggtgtaatg gttgatgttc aagagctgac acttcaggat tcgcaccaag atgtagttgg
     9361 ttcaattact ttcaaagaga taattaacat gctcaaatat ctaccgcagc ttcatagaac
     9421 agttttttgc ctgtatgaaa tcgaaggata cagtcacgaa gagatatcta aagaattaag
     9481 aattccggaa agttcttcaa gggtatacct ttcacgttca aaaaaaagac tgcaggaaat
     9541 tttcaagctc cacttcaaca cgggtcacaa ataatatgga gaaagaaagt atggcgacag
     9601 aagagaatga aatagtggaa attatcaaaa aaaccgcttt agaacatgag gagatctacg
     9661 aagagggcgc ttgggagaat tttgttctta aacaaagaag gaaaagaaga gcattgtttc
     9721 tcaagatttc ttctggagct gcagcatgta tgattattgg gctggcatta ttcttaacat
     9781 ttaaacaacc aactgttcaa aacaataaca acgctacaat cgtcctgaat gataaaaaac
     9841 agacaaagac accaattatt aaacagaact ttttaataaa aactcctgat ttattcaaag
     9901 aagacctaac tattaaaaaa atcattccta aagaagaaat tgcagcaaac agaatagatc
     9961 gctctaaaat agataacggt caaaatttga ttgaagtttc aacagtaaaa
//
LOCUS       DAOTQL010000054         9993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6013_length_9993_cov_4.870698, whole genome shotgun sequence.
ACCESSION   DAOTQL010000054 DAOTQL010000000
VERSION     DAOTQL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6013_length_9993_cov_4.870698"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="P5293_04500"
     CDS             <1..1550
                     /locus_tag="P5293_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HRR49195.1"
                     /translation="YIMMAILTLFISAKFGLSETSTGYIYSAFYASIYVLAVVGGLIA
                     DKTRNYKRTIFWGLILMSFGYLIIAIPTPTPVSSVPLYLSITCIGLLVIAFGNGLFKG
                     NLQALVGQMYDKQDYKDYIAKKFGLDKNGKPKDMRDAGFQLFYMFINIGGFFAPFIAI
                     GVRNWWLKHNGFDYNAQLPELCHKFLSNPAVMNPEQVANLQTYSSQVMLNGEPVSNMT
                     AFCNQYLDVFTRGFHFAFIATIIAILISLVIYVTNKHKFPDPAASKAASTEDKKNHNL
                     SKEEIAMSASEVKQRIYALFAVFGVVIFFWFSFHQNGYSLTYFARDYINLDAINIDLG
                     FTSLKGAEIFQSVNPFFVVVLTPVVMWLFGSLKKKGKEPSTPRKIGIGMGIACAAYLF
                     LLLVSLTLPDKSSLASMSANDLNAMKLTPWVMIAMYFILTCAELFISPLGLSFVSKVA
                     PPHLQGIMQGAWLAATAVGNSLLFIGGLLYTSVPLWACWMVFAIACGLSMIVMLSMVK
                     WLERVAN"
     gene            1645..2211
                     /locus_tag="P5293_04505"
     CDS             1645..2211
                     /locus_tag="P5293_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRR49196.1"
                     /translation="MIPFAKNRSYRRFYQDVSIDEADLIKMVEAARLSPSSRNMQPIK
                     FFITNDRNMNDAIFKNLSWASYLLDWDGPEEGERPSAYIIMLHDKKISSTYSCDNGIF
                     AQSILLQAVDLGFGGCIIGSLKKDAIAKLLQLPEHLEPILAVALGKPKENVIIEDMKN
                     GDVKYWRDDSQNHHVPKRTLDELIYKIK"
     gene            2214..3266
                     /locus_tag="P5293_04510"
     CDS             2214..3266
                     /locus_tag="P5293_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HRR49197.1"
                     /translation="MKIMNPDRPFVIAGPCSAENEEQVIATALGLKEIGIQVFRAGLW
                     KPRSKPGSFEGAGNAGLQWLSRVKKETGLLTCVEVANSSHVKASLKAGIDMIWIGART
                     SANPFAVQEIADTLKGCDIPVLVKNPVNPDVDLWIGAIERFQNSGISCIYAVHRGFST
                     YERGLYRNIPQWYIPIELKRRLSDVPMLCDPSHIAGRRDLILQIAQQSMDLGFDGLMI
                     ESHIDPDSALSDKEQQVTPSELKTILSSIIIRDYEGIPELLKELRREIDEIDDRLLTL
                     ISRRMQVAREIGRCKKSNKIQILQPTRYERLVTQKVSSAEFLQLDPDFIKRVLEAIHE
                     ESVRQQLSITNSSEEI"
     gene            3329..4036
                     /locus_tag="P5293_04515"
     CDS             3329..4036
                     /locus_tag="P5293_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRR49198.1"
                     /translation="MRELLKIFGVALLFLSTCISCKQNVKENPYKLDIVDTKEAYLES
                     IKVDSTNVLVDLQKYIPGIVLDIRYADTNNFTKTKIYTQPRAFLRKPAADSLLKIQQI
                     LAEKGLGLKIYDAYRPYAATLYFYEVYPDTTFVAAPWKGSIHNRGCAVDLSIINLENG
                     SELKMPTPFDSFTEQASHNYNDLDSTALANRALLKDVMLSHGFTLYNAEWWHYNLKQR
                     DNYKLMNLSFEELDAIK"
     gene            4058..5188
                     /locus_tag="P5293_04520"
     CDS             4058..5188
                     /locus_tag="P5293_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49199.1"
                     /translation="MKRSNKKIILLLLFTLFLSVSINAQEVINHSSAETWNPNKVTFP
                     SYITTHRENLILPKIKGYEVISCDFHVHTVFSDGLMWPTFRVLEAWKQGLDCFAMTDH
                     VEYRPHRENLKADLNTSYDLAKEDADQVGLVLIKGAEITRKPKNWGHFNALFIKDANL
                     LEVEDPKEALLAAKKQNAFIIWNHPGWSLDSTLINPFVGEMFKEKIINGIEVYNNCEF
                     YPKAMAWAIDKHLAIIAATDVHGTVEQGTLKQDANQRPMTLVLAKEHSLNGIREALEA
                     GRTMAFFQGYIAAREEIAIWLTEEYLSVKKVYSNEKNDFYTLTNSSSVPFKILFNNKR
                     YVLPALSTISFNVPKGVSKIAVKFENIIIYENKVLAHDLLLQ"
     gene            5386..5955
                     /locus_tag="P5293_04525"
     CDS             5386..5955
                     /locus_tag="P5293_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRR49200.1"
                     /translation="MAVKIRLARHGKKNFSYFHIVVADSRSPRDGRFIERIGFYNPNT
                     DPATIEINSEKALDWLMKGAQPTETCRRILSYKGVLLRKHLLEGVKKGAINQEIADQR
                     WNAWYEAKEAKITQKLSNLELDGRSTKKNTLEAEAKVNAARAEEIAKKKAAEEAAKKA
                     AEAKAAAEVSEPEASATIENEGSSEASEQ"
     gene            5924..6508
                     /gene="rimM"
                     /locus_tag="P5293_04530"
     CDS             5924..6508
                     /gene="rimM"
                     /locus_tag="P5293_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRR49201.1"
                     /translation="MRDLQKHLNNSNSLIGRTLIPVAKVLRSFGTDGRVILKYNLPRP
                     EDENLDRKRPVFIFFDGLPVPFFIEDVNTKGSQQLLIKFEGINTQKFAEEIVGETVYI
                     EKIQKKGSKVKVDKNNGDDITEIIGFKVFDSYEQYIGIIQNIYPYPNNLCIGVHREDK
                     EDELLIPFNEKLIISIDVSHKKAVLNLPKGILDL"
     gene            complement(6426..9254)
                     /locus_tag="P5293_04535"
     CDS             complement(6426..9254)
                     /locus_tag="P5293_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRR49202.1"
                     /translation="MKLLKKIGKILFIGLVTILLLMAVIPYFFPGAVKEKVKNVACSY
                     IDGNVEFKNIGFSFFRHFPHLTFNLDEIVIKGPLPFQNDTLLSGKSVSTSINLLPLLK
                     GNVEIDGIYLDEVFLNLITDKNGKNNYEIVQLSQSNDTLTVQQERDASFKGSVNLKNI
                     VISNSSLRYFDSPNKMALEIDGLNYRGKGNIKDANFLLLSLLNIKELSFAQNGTKMID
                     KKGIKANFTTNVNLDNFKISLLKNKLKIAGVKTEFHGYYSPTDNGYSVDMELNTSDNS
                     FKELFSLLPEKYSEWLANTKIKGNASIQLQVNGVANDSLGTFPDLIVAIDINKGSLSH
                     AKAPVPLNAINLSCQIILPSLNTEQVAVKVNNLSFNLGGDKNKLTLEMKGLSSPLIKV
                     DMDGSIDLETLSNAMGFENYKAEGKLDYKILLDGIYNPELRLLPVIDAKIRLKEGAVS
                     TTFYPGTLCDLNADIEIKDVKGNYSDLKIVADPFNFNFDGKPFTLTAKMENFDNLKYK
                     ITSNGQLNLDNLYRLFAIDGITLKGEISTDLYLSGNQQDAKAGRFNKLDNRGQIGFRN
                     FEFMSQDYPFPFRVPDAVIIIDKDKILLSNAILKYRDNTFRIDGYAKNFMGYFFEGGK
                     LTGNATLTADSVNLDDFRFLFEGEEFEGEEISSIDSAGVIRIPSNFNLSLNAHVHKLN
                     FAGTRIDDINGGVRLDNEEAKLKNTTLQIAGARFLLSANYKPLSHEKALFDMSVRADS
                     FDIHRAYNEIPLFKELVRSAESIYGKVSLQYSLKGELDREMSPIYPSLKGSGYIRLDE
                     VKINGLRVLSEISKATGRDSLDNPELKGVLVKSSINNNIVTIERTKMRIFGFRPRFEG
                     QVSLDGRLNIKFRLGLPPFGIIGIPVTVTGTMDNPKVHLRRSQSGDILYETEPQEEAD
                     YIKDQVSLWEDLTLPFCVKHQLKLSIFH"
     gene            complement(9256..>9993)
                     /locus_tag="P5293_04540"
     CDS             complement(9256..>9993)
                     /locus_tag="P5293_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49203.1"
                     /translation="EANPLIPENIVALQTLVVEKGCDVGIIFDGDGDRVIFVDENGRF
                     ISPDLMIAVLGHYFLEERGERGKVLQDIRSSKSVGEYLVPMGAEMVTWKVGRAYAAHK
                     LREIDGIFGGELAGHYYFKDFFYSDSGIMASLIILGIISGMKKKGVTLSQLISRIEKY
                     KNSGEINFKIEQKQKAMDRVRDYFISIEEPVAYYDFDGYRVEFPQWWFNIRPSNTEPY
                     LRFIAEASTAELLESKISKVKELLEDL"
BASE COUNT         3170 a         1939 c         1822 g         3062 t
ORIGIN      
        1 tctacattat gatggcaata ttgacattgt tcatctctgc aaaattcggt ctgtccgaga
       61 cctctactgg ctatatttac tccgctttct acgcatcaat ctatgttctt gctgtcgttg
      121 gaggtttgat agccgataag actcgtaatt ataagcgtac tattttttgg ggacttattt
      181 tgatgtcctt tggttatttg attattgcta ttcctactcc tactcctgtc tcaagtgttc
      241 ctctttatct ttcaatcact tgcataggtt tgttggtaat tgcctttgga aacggacttt
      301 ttaagggtaa ccttcaggca ttggttggac aaatgtatga caaacaagac tacaaggatt
      361 atatcgcaaa gaaattcggt ctggacaaaa atggcaagcc taaggatatg agagatgctg
      421 gtttccagct attttacatg ttcataaata tcggaggttt ctttgcccct tttattgcta
      481 ttggtgtaag aaactggtgg cttaaacata atggttttga ttataatgct caactccccg
      541 agctttgcca caaattttta tcgaatcctg ctgtaatgaa tcctgaacag gtagctaatc
      601 ttcagactta ctcctcccaa gtaatgttaa atggtgaacc tgtttcaaat atgactgctt
      661 tttgcaatca atacctcgat gtatttacaa gaggtttcca ttttgctttt attgcaacaa
      721 taatcgcgat attaatttct ctggtaatat atgtaacaaa taaacataaa tttccagatc
      781 cggctgctag taaagcagct tcaactgaag ataaaaagaa ccacaatttg tccaaagaag
      841 agattgcaat gtcagcttct gaagttaaac aaaggattta tgcactattt gctgtttttg
      901 gagttgttat ctttttctgg ttctctttcc atcaaaacgg ttattctctt acctattttg
      961 cgcgtgacta tattaatcta gatgcaataa atattgatct tggttttaca tctctaaagg
     1021 gagctgaaat cttccagtcc gtcaatccat tctttgttgt tgtcttaacg cctgttgtaa
     1081 tgtggctgtt cgggtctctt aaaaagaaag gaaaagaacc ttcaacccct cgaaagatag
     1141 gaattggtat gggtattgct tgtgctgcat atcttttctt gcttttagtt tctcttacat
     1201 tgcctgacaa atcatcgctg gcaagtatgt ctgcaaatga tctgaatgcc atgaaactta
     1261 ctccatgggt aatgatcgcc atgtatttta ttcttacgtg tgccgagttg tttatctcgc
     1321 cattaggttt gtcatttgta tctaaagttg ctcctccaca tcttcagggc ataatgcaag
     1381 gagcatggct cgcagctact gctgtaggaa attctctttt gttcattggt ggccttcttt
     1441 atacatctgt ccctctatgg gcatgctgga tggtatttgc aattgcatgc ggactctcaa
     1501 tgatagttat gctttcaatg gttaaatggc ttgagagagt tgccaattag aatagtaaca
     1561 tttctattta agattatagg aagccgaact gaaataccat tttagtcggc ttcctttgta
     1621 acagataaaa tcaacaatta tattatgata ccatttgcta aaaacagaag ttacagaagg
     1681 ttctatcagg atgtttcgat tgatgaggct gatcttatca agatggtaga ggctgcaagg
     1741 ctctctcctt cttcaagaaa catgcaacct attaagtttt ttataacaaa cgacagaaat
     1801 atgaacgacg cgattttcaa aaatctttca tgggcaagtt atctccttga ttgggatggc
     1861 cctgaagaag gagaaagacc gtctgcatat attataatgc tccatgataa aaaaatctct
     1921 tcaacatatt catgtgacaa tggaattttt gcacaaagca ttcttcttca ggctgtagat
     1981 cttggctttg gtggctgcat aatcggttcc cttaagaaag atgctattgc aaagttgctc
     2041 caacttcctg agcatcttga gccaattctt gctgtagctc ttggcaaacc aaaagaaaac
     2101 gttattattg aagacatgaa aaatggagat gtcaagtact ggagagatga cagccaaaat
     2161 caccacgtcc ccaaaagaac acttgacgag ctgatttata agataaaata aaaatgaaaa
     2221 tcatgaatcc tgacagacct tttgtgatag ccggcccatg cagtgcagaa aacgaggagc
     2281 aggtaattgc aactgcttta gggctaaaag aaatcgggat tcaagttttc agagccggcc
     2341 tctggaaacc tcgctccaaa cctggatcat tcgagggagc aggcaatgct ggcttacagt
     2401 ggttatcaag agtcaagaaa gaaacagggt tgcttacttg tgtggaggtt gctaactctt
     2461 ctcatgttaa agcatctctt aaagccggca ttgatatgat ctggattggc gcaagaacaa
     2521 gcgcaaatcc ttttgccgtt caggagatag ccgatacact gaaaggttgt gatatcccgg
     2581 tacttgttaa aaatccggtt aaccccgatg tggatttatg gataggagca atcgagagat
     2641 ttcaaaacag tgggataagt tgcatatatg cagtacacag aggattcagc acttatgaga
     2701 gaggacttta caggaatatt cctcaatggt atattccaat tgaactcaaa cgcagacttt
     2761 cggatgttcc gatgctatgc gacccaagcc acatagcagg caggcgtgac cttatacttc
     2821 aaatagccca gcaatctatg gatctgggct ttgacggact gatgatagaa tcgcacatag
     2881 acccagattc tgcattgagc gataaggaac aacaagtcac tccgtcagaa ttaaagacaa
     2941 tactctcatc cattataatc agagattatg aaggaatccc tgaattattg aaagagctac
     3001 gaagagagat tgatgagata gatgacagac ttttaacatt gatttcgaga aggatgcagg
     3061 ttgccagaga gatagggagg tgcaagaaaa gcaacaagat ccagatctta cagccgacgc
     3121 gctacgaacg ccttgtaacc cagaaggtat caagtgcaga atttctgcag ttagatcctg
     3181 attttatcaa aagggtactt gaggcaatac atgaagagtc agtcaggcaa cagttgtcaa
     3241 ttacaaacag tagcgaggaa atttaaattg acagtatctg tttttttgta aatttacaaa
     3301 atattcataa cctaataaac tcgacaacat gagggaacta cttaaaattt ttggtgttgc
     3361 cctgcttttt ctctccacct gcatctcctg taagcaaaat gtcaaggaaa atccatataa
     3421 actggacatt gttgacacaa aagaggctta tctcgaaagt ataaaagttg acagcacaaa
     3481 tgtgcttgta gatttacaaa agtacatacc cggtattgtg ttagatatca gatatgctga
     3541 caccaataat ttcactaaaa caaaaattta tactcagcca agggctttct tgagaaaacc
     3601 tgctgccgac tcactcctga aaatacaaca aatcttggct gaaaaaggac tcggtcttaa
     3661 aatttatgat gcatacagac cttatgccgc aacactctat ttttacgaag tatacccaga
     3721 caccacattt gtcgcagctc catggaaagg gtcaattcat aacagagggt gcgcagtgga
     3781 tctttcaatt ataaaccttg aaaacggttc tgaattgaaa atgccgacac catttgacag
     3841 cttcacagaa caagcttcgc ataattataa cgaccttgac tctactgctc tggcaaacag
     3901 agcacttcta aaagatgtaa tgttgagtca cggcttcaca ctttacaatg ccgaatggtg
     3961 gcattacaat ctaaaacaaa gggacaatta caagctgatg aatctctctt tcgaggaact
     4021 tgatgcaatt aaataatcct atcactataa ttgaacaatg aaaagatcaa acaaaaaaat
     4081 cattctgctg ctcctgttta ctctttttct atcagtcagc ataaatgctc aggaggttat
     4141 aaatcactct tcggctgaaa cttggaatcc taataaggtg acctttccgt cctacataac
     4201 aactcatcgt gaaaacctta ttttgccaaa aatcaaagga tatgaggtta tttcctgcga
     4261 ctttcatgta cacaccgttt tttctgacgg ccttatgtgg cctactttca gagttcttga
     4321 agcctggaaa cagggtcttg actgctttgc tatgacagat cacgttgagt acagaccaca
     4381 cagagagaat cttaaggcag atctgaacac gtcttacgat ctggcaaaag aagatgctga
     4441 ccaagttgga cttgtgttga taaaaggtgc tgaaataacc cgtaaaccca aaaattgggg
     4501 acattttaat gctcttttta tcaaagatgc caatcttcta gaggttgaag atcctaaaga
     4561 ggcattgctt gcagcaaaaa aacaaaatgc ttttatcata tggaatcacc ctggatggtc
     4621 tttagacagt actctcataa atccgtttgt cggggaaatg ttcaaggaga aaataataaa
     4681 cggtattgaa gtctataata attgtgaatt ttatcctaaa gccatggcat gggcaattga
     4741 taaacatctt gcaattattg ccgcaacgga tgtccacgga acagttgaac aaggcacact
     4801 caaacaagat gcaaatcaga gacctatgac acttgtcctg gctaaagaac acagtttaaa
     4861 tggcatcaga gaggcacttg aggccggaag aacaatggcg ttttttcaag ggtatattgc
     4921 tgccagggaa gaaattgcca tatggcttac agaggaatat ctgagcgtca aaaaggtata
     4981 tagcaatgag aaaaatgatt tttacacatt gactaacagt tcttcagtac cgtttaaaat
     5041 attatttaac aataagagat atgtgttacc tgccctctca acaatctctt tcaacgtgcc
     5101 taaaggggta tcaaagattg cagttaaatt tgagaatatt attatctatg aaaataaagt
     5161 tttggcacat gatttgttat tacaatagac gtgaagtttt ttcataggtt aagttttagg
     5221 ttaagtgtaa aatgaaagag ggtgtctggc aaacaggcgt cctttttcat tttacaaacc
     5281 aaactctaaa caaaattgca ttttcaataa tttgttgtac atttgcacgc cttttctcga
     5341 gaagattagg aagttaaaca ataataactt aaaaaacaca gtattatggc tgtaaaaatt
     5401 cgtcttgcca gacatggcaa aaaaaacttt tcttattttc atattgttgt agctgacagt
     5461 cgttcacccc gtgacgggcg atttattgaa cgtataggat tttataatcc caatacagat
     5521 cctgcaacta ttgaaataaa ttctgaaaaa gcactcgact ggcttatgaa aggtgcacaa
     5581 cctacagaaa cctgccgcag aatactatcc tataaagggg tgttgttgag aaagcatctg
     5641 ctggaaggtg taaagaaggg ggccattaat caagaaattg ctgatcaaag atggaatgca
     5701 tggtatgagg caaaagaggc taagattact caaaagctgt caaacttgga actcgacgga
     5761 cgctctacca aaaagaacac tcttgaagcc gaggccaaag tcaatgctgc acgcgcagag
     5821 gaaatagcca agaagaaagc tgcagaggaa gctgctaaaa aagctgccga ggcaaaagct
     5881 gctgctgaag tttcagaacc ggaggcatct gcaacaattg aaaatgaggg atcttcagaa
     5941 gcatctgaac aatagcaata gccttattgg cagaacactg attcctgttg caaaagttct
     6001 aagatcattt ggaactgacg gtagagttat tttaaaatat aacttacctc gtccggaaga
     6061 tgaaaatttg gatagaaaga gaccggtgtt tatctttttc gatggattgc cggtcccttt
     6121 ttttattgaa gatgtcaata ctaaaggttc acaacaacta cttataaagt ttgaaggaat
     6181 taatacccaa aaatttgccg aagagattgt tggtgaaact gtttacatag aaaaaataca
     6241 aaagaaaggc tcaaaagtaa aggtcgacaa aaataatggc gacgatatta ccgagataat
     6301 tggatttaag gtgtttgatt cttacgaaca atatattggc attatccaaa atatatatcc
     6361 atatccaaat aatctttgta taggagttca cagagaagat aaagaagatg aactccttat
     6421 tccttttaat gaaaaattga taatttcaat tgatgtttca cacaaaaagg cagtgttaaa
     6481 tcttcccaaa gggatacttg atctttaatg taatctgctt cttcctgagg ttctgtctcg
     6541 taaagaatat caccgctctg actgcgtctg agatgtactt ttggattatc cattgtacca
     6601 gtcacagtga caggtatacc tattatgcca aaaggaggaa ggcccagtct gaacttgata
     6661 ttcagcctgc cgtcgagaga aacttgtcct tcaaatcttg gacgaaaacc aaatattctc
     6721 atcttggttc tctcaatcgt tacaatattg ttattaatac tgcttttaac caaaactccc
     6781 ttaagttcag gattgtccag actgtctcga ccggtagctt tactaatttc actcagcacc
     6841 ctgagaccgt ttatcttcac ctcgtccagt cttatatagc cagatccttt aagagatgga
     6901 taaatcggag acatctctct gtccaactcg cctttgagtg aatattgcaa tgataccttg
     6961 ccgtatatac tttctgcact tcttacaagc tctttgaaca gcggtatctc attataagct
     7021 ctgtgaatat caaaggagtc tgctcttaca ctcatatcaa acaatgcctt ttcatgtgaa
     7081 agcggcttgt aatttgcact taataagaac cttgcgccag ctatttgaag tgttgtattt
     7141 ttaagtttgg cctcctcatt atcaagtctg actccaccgt taatgtcgtc aattctggtt
     7201 ccggcaaaat tcaatttatg cacatgtgca tttaatgaaa ggttgaaatt ggaaggaatt
     7261 ctgataacac ctgcactatc aattgaactg atttcttctc cctcaaattc ttctccctca
     7321 aataaaaaac gaaaatcatc cagattaaca gaatctgcag taagtgttgc gtttcccgta
     7381 agtttacctc cttcaaaaaa gtagcccatg aaattcttag catacccgtc aattctgaaa
     7441 gtattatccc tatattttaa tattgcattg ctaagcaata tcttgtcctt gtcaataatt
     7501 atgacagcgt caggaactct gaaagggaaa ggataatctt gactcataaa ttcaaaattg
     7561 cgaaatccaa tttgccctct gttgtccaat ttattgaatc tcccagcttt cgcatcctgt
     7621 tgattacctg aaagataaag atcggttgat atctctcctt tcagcgtaat tccatctatt
     7681 gcaaaaagcc ggtacaaatt atctaaattt aactggccat tagacgtaat tttatatttc
     7741 aaattgtcga aattttccat tttagcagtc aatgtaaacg gctttccgtc aaaattgaaa
     7801 ttaaaaggat ctgctacaat cttaagatct gaatagttgc ctttaacgtc cttaatctca
     7861 atatccgcat ttaaatcgca caaagttcct ggataaaagg tagtagaaac agccccttcc
     7921 ttaagcctga tttttgcatc aataactgga agcaatctga gttcaggatt ataaatacca
     7981 tctaacaaaa tcttgtaatc cagttttcct tctgctttat aattttcaaa tcccatagca
     8041 ttagaaagag tctccaagtc aatagaccca tccatgtcta ccttaattag aggtgacgaa
     8101 agtcctttca tttctaaagt taatttattc ttatcacctc cgagattaaa ggataaattg
     8161 tttaccttaa ctgcaacttg ttccgtgttg agagatggaa gaataatttg acaactaaga
     8221 ttaatggcat taagtggaac aggtgctttt gcatgtgata aacttccctt attaatatca
     8281 attgccacaa taagatctgg aaaggttcca agtgaatcat tcgcaactcc attgacttgt
     8341 aattgaatag aagcattccc tttaattttg gtatttgcaa gccattctga atacttctca
     8401 ggcaaaagtg aaaaaagttc cttaaaactg ttatcggaag tattcaactc catgtccaca
     8461 ctatacccat tgtcggtagg tgaataatat ccgtgaaatt ccgttttaac ccctgcaatc
     8521 tttaatttgt ttttcaacaa cgaaatctta aaattatcaa gattgacatt tgtggtaaaa
     8581 ttggctttaa ttcctttttt gtcaatcatt ttagtaccat tttgggcaaa agacaactcc
     8641 ttgatgttaa gcagtgataa aagtaaaaaa tttgcatcct taatatttcc cttccctctg
     8701 taattgagcc catcaatctc taaggccatt ttgtttggac tgtcaaaata tcgtaaactg
     8761 ctattggaaa tcacaatgtt tttcagattg accgagcctt taaaagaagc atctctctct
     8821 tgctgtacgg ttaatgtatc atttgactgg ctcaattgga caatttcata gttgtttttc
     8881 ccattcttat cagtaataag attcagaaat acctcgtcga gatatattcc gtcaatttca
     8941 acattccctt ttagcaaagg caacaagttt atgcttgtag agactgattt acctgacaaa
     9001 agagtgtcat tttgaaatgg aagtggccct tttattacta tttcgtctaa attaaaagtc
     9061 aggtgaggaa aatgacgaaa aaatgaaaaa ccgatgtttt taaattctac atttccatca
     9121 atatagctgc atgcaacatt tttaaccttc tctttgacag ctcctggaaa aaaataagga
     9181 atgacggcca taagcaaaag aatggtgaca agtcctatga acaagatctt tcctattttt
     9241 tttaatagct tcatattaca aatcctctaa caactcctta acttttgaaa tctttgattc
     9301 aagaagctcc gctgtagatg cctctgcaat aaatctcaaa tatggttctg tatttgaagg
     9361 cctgatatta aaccaccatt gagggaactc aactctatat ccatcaaaat cataataagc
     9421 aaccggttct tctatagaga taaagtaatc tctaactctg tccatagctt tttgcttttg
     9481 ctctatctta aaattaatct ctccagaatt tttatatttt tctatccttg aaataagctg
     9541 agaaagagtt acaccttttt ttttcatccc tgatattatt cccaatatta tcaaagaggc
     9601 cattatgcct gaatcactgt aaaaaaaatc tttgaaataa tagtgacctg cgagctctcc
     9661 tccaaaaatc ccgtcaatct ctcttaattt atgagcagca tatgctcttc caactttcca
     9721 tgtaaccatt tcggcaccca ttggaacaag atattcgccg acagacttag agcttcgtat
     9781 atcctgcaag actttccccc tttctcccct ttcttcaaga aaatagtgtc caagaacagc
     9841 aatcatcaga tcgggagaaa taaaacgtcc attttcgtca acgaatatca ccctgtcacc
     9901 gtctccgtcg aaaattatgc ctacatcaca acccttctca actacaagag tttgaagggc
     9961 aactatgttt tcaggaataa gaggattggc ttc
//
LOCUS       DAOTQL010000055         9889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6107_length_9889_cov_5.311165, whole genome shotgun sequence.
ACCESSION   DAOTQL010000055 DAOTQL010000000
VERSION     DAOTQL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6107_length_9889_cov_5.311165"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..268
                     /locus_tag="P5293_04545"
     CDS             92..268
                     /locus_tag="P5293_04545"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRR49204.1"
                     /translation="MRETVESIFRELQDFKDDAKVADYIPALAKVNPDSYAISVSILG
                     KENISVGDYSDNGV"
     gene            945..2054
                     /gene="pdhA"
                     /locus_tag="P5293_04550"
     CDS             945..2054
                     /gene="pdhA"
                     /locus_tag="P5293_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRR49205.1"
                     /translation="MTIFNEYDPIKKQIFRIMDDDGKIINKTWMPEIDNERLVKAYKD
                     MLFSRIADLQIVSYQRQGRIYTYPPNYGQEAISGAAGAIIKDRDWMVPAFREIGTMMA
                     KGVSLKEIFLYYMGYEEGSNFKNAEFVLPVSVPIASQLIHASGIGYAIKYKKEEKVVY
                     AFVGDGGTSEGDFHEAVNFAGVWKVPVIFIIQNNQYGISTPVRLQTASESLAIKSIAY
                     GVKGIQVDGNDFFAMYKTLEESAKACLAGEGPILIEAVTYRKGAHTTSDDPSRYRTKE
                     EEESWEKRDPLKRLKNYLISKELWSEREEEKLIPQYREEVDRQFIEAENFGPYPKEDV
                     FKHLYADMPDDLLEQMNQYDRFLQWKETKVNNKTT"
     gene            2061..3038
                     /locus_tag="P5293_04555"
     CDS             2061..3038
                     /locus_tag="P5293_04555"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HRR49206.1"
                     /translation="MKIMNMVNAINDALDIKLKEDRGVIVYGEDVGVEGGVFRVTEEL
                     QQKYGKERVFDSPLAESGIMGTAIGMAVAGLRPVVEMQFCGFIYPAFNQIISHASRMR
                     NRSRGKYETPIVIRMPYGGGINALEHHSESMEAIFGHIPGLKVVIPSTPHDAKGMLIS
                     AIESNDTILFMEPKRIYRAIKQEVAEGKYTIPLGKANVVTQGTDVTVVAYGAMVREVQ
                     RAMVMAKEQGISIELIDLRSIYPIDRETIAKSVKKTGRIITVTEGPRSFGVGSEISQI
                     AIEEAFLSLEAPPSRISGFDTIVPLPRGEYHYMQDPYKILYEIEKIVKY"
     gene            3043..4353
                     /locus_tag="P5293_04560"
     CDS             3043..4353
                     /locus_tag="P5293_04560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRR49207.1"
                     /translation="MRYIFKFPDIGEGLDEGTIVEWHVEKGQSVKMGDPLCTMETDKV
                     VTAIPSPKDGIIAARFGKTGEVIHVGNALVEIDIEGIDGEAAVEEANKVEVVNEEGAG
                     VVGTLEVAGNNFVLSASNEGVQKMDDDVSRRGGKVLATPVARAIAKDLGVKIDLVKGS
                     GPAGRVTKEDIIRFSEDQGSAATTTRSTSDILVVNQTSAPNNPENLVEIENMSQIRKS
                     IAKHMSQSKHNAAHMTLFEEVEISELERIRTKYKEELLKEGVKLTYLAFIVKAVALSL
                     KKFRALNSEMNFEGGKMIYKKYYNIGIATDTDKGLVVPVIKNADQLSVKEIAQEITHI
                     SNKARQGKLTLEDMKGGTFTITSYGSIGGLFAVPVINYPEAAILGVGRISKQPVVKGN
                     ELAVGLVLPLSLSVDHRIADGGETARFMNSVLSYISDPVTLLFN"
     gene            4368..5720
                     /gene="lpdA"
                     /locus_tag="P5293_04565"
     CDS             4368..5720
                     /gene="lpdA"
                     /locus_tag="P5293_04565"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRR49208.1"
                     /translation="MYDIIIIGSGPAGYVASIRAGQMGLRCAIVEKEKIGGMCLNWGC
                     IPSKSMIESVKFYQRIIKDSSRFGIEGIDRKVVTFSWNTAIKRADTIVKRLTGGVEFL
                     LKKNGVEIITGEARIISANSVLVENRLLKTKNIIIATGSTSLPIDNLFENLVIEPKDL
                     FKEREIPENIVITGNAPVAIELAQLFRMMERSVTLVSTADHIMPKADDYIRKYMENKL
                     KKDKIEILYNIGLSSTAGSWNDGVLNLEGKEVKCDLIINASSRKGNLIKSDIEIVIEN
                     GFYSVNEDCETSVKGIYAVGDVNGVSQFAHIGSAQGLHVVNLLKGIRQRLDIKKLPMN
                     MYSVPEAAQIGLTEQEAKEEGYDYKISEFPLSANGKAMAEGQAEGFVRLISEKKYGEV
                     LGVQIVAPNATDMINEAAAYMQLESTVYDIAQTVHTHPTVSEVFMEAGFEAVDKAIHK
                     "
     gene            5722..6345
                     /locus_tag="P5293_04570"
     CDS             5722..6345
                     /locus_tag="P5293_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49209.1"
                     /translation="MEPIVNKYTLPDAAILDSPDEYSYKVWQPDKLYAVLGRANNIDD
                     SLITGNITKDNVTVLKRPSGGEAVILSPRMLVISAKMKFYKEMNTKQIFNRINTLLID
                     RLSALGIKGLYSRGISDLSINDRKILGSSMYLNKDVIFYHAVLNISEDVSLLSKYLKH
                     PTREPDYRKGRSHLEFVTSIHKEGYPFTSRELSSVLTLALQQILFSK"
     gene            6731..6976
                     /locus_tag="P5293_04575"
     CDS             6731..6976
                     /locus_tag="P5293_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49210.1"
                     /translation="MNNDTKRYLKFLITPVAILLLGIVIGRSCFIFLRNWAWIPVVLT
                     YWIAIGLVLYLDYKKKGTTPVYSCTKIDDEPQIAGVL"
     gene            6948..7154
                     /locus_tag="P5293_04580"
     CDS             6948..7154
                     /locus_tag="P5293_04580"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR49211.1"
                     /translation="MNRKLREYYNHTTGGNFMSAVLNDKGEFIGVNFDRIWQGVVSDY
                     RYNSTPSQAIAVDIRYIMSLFDKL"
     gene            7245..8120
                     /locus_tag="P5293_04585"
     CDS             7245..8120
                     /locus_tag="P5293_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRR49212.1"
                     /translation="MNTYVVIAIIVLVIFAAGIRIVRPTHRALIERLGKYYKFANTGF
                     HWIIPVIDKIYKVNVTERMVDAEPQEIITNDNLNAKVDAQVYFRVKSDEESVKGSIYN
                     VNNFQWQIVNLARTTLRNIIGTLTLKSANSERGKINSDLYKTLHDETREWGIEIVRTE
                     LKEIDPPKDVQETMNKVVKAENEKIAAIDSATAAETVADGVKRAKIKEAEGYKQSKIL
                     HAEGEAEAIRLVNEAADKYFIGNAQLLKRLETLEGALSENSKIVIPTGTDLVNIISDM
                     AGVNVLPLEKRKKSE"
     gene            8251..8706
                     /locus_tag="P5293_04590"
     CDS             8251..8706
                     /locus_tag="P5293_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR49213.1"
                     /translation="MLPTIRKATADDISQIVYFQMNMALETERLELNRDILTAGVTNV
                     LTGEKGASYYMAEIDGTLAGMLMITLEWSDWRNGWVWWIQSVYIRPEFRGKGLYKMLY
                     FHIVNLAKKSDDVMGIRLYVDKTNTNAQKVYKALGMNGDHYLVYEWMKD"
     gene            complement(8805..9131)
                     /locus_tag="P5293_04595"
     CDS             complement(8805..9131)
                     /locus_tag="P5293_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRR49214.1"
                     /translation="MNIFISNLSYSVKDAELNQLFSEFGEISSAKVIMDRETGRSRGF
                     GFVDMQDEEAGQKAINELNGCEYDGKVINVNVAKPKENRDNSRGGYNRGGGNDRRGGY
                     ENRRRY"
     gene            9317..>9889
                     /locus_tag="P5293_04600"
     CDS             9317..>9889
                     /locus_tag="P5293_04600"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HRR49215.1"
                     /translation="MRKKDLKKLILALVLITTVQGIGIDIYVNSAEGGIWEGLSIFKY
                     FTLQSNALVMLYALLALINFRSMTEKEWFKRALTPVTSYIMLTGITFLIILAPTSNAQ
                     GLQRVASDLLHYLVPSLMFFYWIVFEERELQYSYIWSWIIYPFVFMFWGLYLAIMKED
                     YLYPFFDINKLGILIVPYLAGMTIAVFLICS"
BASE COUNT         3233 a         1780 c         2146 g         2730 t
ORIGIN      
        1 gccatctttt taaaaagagg tgtccagaaa gttttggaca cctctttttt tatttaaatt
       61 tgaagaatac aaataattac taactatagt gatgagagaa acagttgaga gtatcttccg
      121 tgaactacaa gattttaagg atgatgccaa agttgccgat tatattccgg ctttagcgaa
      181 agtaaatcct gattcgtatg caatctcagt atcaatactg ggaaaagaaa atatatctgt
      241 aggagattat tcggacaacg gagtgtaaat gtggcgggga catggtgcaa gtgttttgtc
      301 tatttattgt gttaataatg tgagtattac taattattca ttagacccca actaaatctt
      361 tagttttcaa tttttaagtt actactaatc aggcacatgc ttcataccgt ggcagtgtcc
      421 cactgaagtt ccccatgttc tttaagtact caattgtgta caatgactta tattttatgg
      481 aaattacttc ttcataatca gcctgccatg tcatgacagc aaagcagaag ggtgcattgt
      541 ttttttagcc aatttcgaag ctgtttttta ggaaaaagtg cgaggaccgt ccaacgaccg
      601 aagggaggcg ttcatcaaca ccttgaaaac aagcaagaaa tggcgtgaag aaaaaacaca
      661 gtacccgatg attatgctgt ttctgaccgg ttagcagtga aggagtgaat agtttttaga
      721 tttacaccat gattttcaat aggaggatta tggtgtattt ttgtttataa aactaagagt
      781 ggcagccgat taccttagac tttggacatt tagacattag acgtttaatg cactccatca
      841 tttttcacta ttagttagct tcacatgtgg ggaacttcag cagagtgtgc ctgaacaaaa
      901 cctggtaatt cgttgttaat tgaattgata aatcacaatt caacatgaca attttcaacg
      961 agtacgatcc aataaaaaaa cagattttca ggataatgga tgatgatgga aaaatcatca
     1021 ataagacctg gatgcctgaa atcgacaatg aacgtcttgt taaggcatac aaggacatgc
     1081 ttttctcaag gattgcagat ctacagatag tttcttatca aagacagggc agaatctaca
     1141 catatccccc aaactatgga caggaagcaa tctccggcgc agcaggcgca ataataaagg
     1201 acagggactg gatggttccg gcattcaggg aaataggaac aatgatggca aaaggagtgt
     1261 ctctcaagga gatctttctt tactatatgg gatatgaaga aggctccaac ttcaagaatg
     1321 cagaatttgt actacctgtg agtgtcccta tcgcttctca gctaatccat gcttcaggta
     1381 ttggatatgc aattaaatac aagaaagagg agaaggtggt gtatgctttc gttggagatg
     1441 gcggcacatc cgaaggtgac tttcatgaag cagtaaactt tgccggagtc tggaaggtcc
     1501 cggtaatatt cattatccaa aataatcaat atggaatttc aacaccagta cgtttgcaga
     1561 cagcgtcaga atcacttgcc atcaagtcaa tagcttatgg ggtcaagggt attcaggtgg
     1621 acggaaatga cttctttgcc atgtacaaaa ctttggaaga gagcgctaaa gcatgccttg
     1681 caggggaagg tcctatcctt attgaggctg tgacttacag aaagggggca catactacat
     1741 ctgacgatcc atcaagatac agaacaaaag aggaggagga atcttgggaa aagagggatc
     1801 cacttaaaag gctcaagaat tatttaatat caaaagaatt atggagtgag cgagaggaag
     1861 agaagcttat tcctcaatac agagaagagg tcgacagaca attcattgag gctgaaaatt
     1921 tcggaccata cccaaaagag gatgtattca aacacctata tgcagacatg ccggatgatc
     1981 ttctggaaca gatgaaccag tacgaccgtt tcttgcagtg gaaagagaca aaagttaata
     2041 acaagacaac gtagcaagca atgaaaataa tgaatatggt caatgcgata aatgacgcat
     2101 tggatataaa actaaaagaa gacaggggag ttattgtata tggggaagat gttggcgtag
     2161 aaggtggagt ctttcgtgtt acagaggaac ttcaacagaa atatggtaaa gagagagtat
     2221 tcgactctcc tcttgcagaa tcaggaataa tgggaactgc aataggtatg gcggttgcgg
     2281 gactcaggcc tgtggttgaa atgcaattct gcggatttat ttatccagca ttcaatcaga
     2341 taataagtca tgcctcccgg atgcgcaacc gctcaagggg aaaatatgaa actcctatag
     2401 tcatccgaat gccttatggt ggtggtatta atgcacttga acatcacagt gagagcatgg
     2461 aggctatctt cggccatatt ccaggactta aagttgtcat cccatcaaca ccgcacgatg
     2521 ccaaaggtat gctaatctcg gcaatagaaa gtaatgacac tatcctcttt atggagccaa
     2581 aaagaattta ccgagccata aaacaagagg ttgctgaagg taagtacact ataccattag
     2641 gaaaagccaa tgtcgtgact caaggaacag atgtaacagt agtagcatac ggtgcaatgg
     2701 taagagaagt acaaagagcg atggtaatgg caaaggaaca gggaataagt atagagttga
     2761 tagatcttcg ttctatctat cctattgaca gggagactat agcaaaatcg gtcaagaaga
     2821 ctggccggat aattacggta acagaggggc ctcgttcgtt cggagtgggt tcggaaatca
     2881 gtcagattgc tattgaggaa gcattcctta gtcttgaggc acctccctca agaatttccg
     2941 gatttgatac cattgtgcca ctaccaaggg gagaatatca ttacatgcaa gacccataca
     3001 agattctgta cgaaattgaa aaaattgtaa aatattaaag ttatgagata tatatttaaa
     3061 tttcccgata ttggagaggg tctcgatgaa gggactattg ttgaatggca cgttgagaaa
     3121 ggccagtcgg tcaaaatggg agatccactt tgcacaatgg agacagacaa ggttgtaaca
     3181 gccatcccat cgccaaaaga tggtattatt gctgcaaggt ttggaaaaac cggcgaagtc
     3241 attcatgtag gaaatgcgct cgtagaaatt gatattgaag gtattgatgg agaagctgcc
     3301 gtagaggagg caaataaggt agaggttgtg aacgaagagg gtgcaggtgt tgtcgggaca
     3361 cttgaggttg caggtaataa ttttgttcta tccgcaagta acgaaggcgt acaaaaaatg
     3421 gatgatgatg taagcaggcg cggtggaaag gttctggcca ctcccgtggc ccgcgcgatt
     3481 gcaaaagatc ttggtgtcaa gatagacctg gtaaaaggct ccggtcctgc gggcagggtt
     3541 acaaaggaag atattatccg cttttcagag gatcaaggtt ctgcagcaac aacaacaaga
     3601 tccacctcag atattcttgt ggtaaatcaa acctcagctc caaacaatcc agaaaatctg
     3661 gttgagatcg agaatatgtc acagatacgc aagagcattg caaagcacat gagccaaagc
     3721 aagcacaatg ctgcacacat gacattgttt gaggaggtag aaatatcgga actggagaga
     3781 atacgcacta aatacaagga agaacttcta aaagaggggg taaaactgac atatcttgcg
     3841 tttattgtca aggctgttgc cctatctctc aaaaaattcc gtgcactcaa ttcagaaatg
     3901 aactttgagg gcggtaaaat gatttataaa aaatattata acatcggaat tgcaaccgat
     3961 acagacaagg ggctggttgt acctgtcata aagaatgcag accagctctc tgtaaaagag
     4021 attgcacagg agataaccca tatatccaat aaggcccgtc agggcaaact tactcttgag
     4081 gatatgaaag gaggcacatt tactatcacg agctatggca gcataggtgg acttttcgca
     4141 gtacctgtga taaattaccc tgaagcagcc atccttggtg taggtagaat ttcaaaacag
     4201 ccagttgtaa agggaaatga gcttgctgtt ggtcttgttc tccctctctc ccttagtgtt
     4261 gaccatcgca tcgcagacgg cggggagact gcccgcttta tgaactcagt attgagttat
     4321 atttcagatc ctgtcactct actttttaac taattatgga aaaaatcatg tacgatataa
     4381 taatcattgg ttccgggcca gcaggctatg tagcctcaat ccgtgccggg cagatgggcc
     4441 tccggtgcgc catagttgag aaggaaaaaa taggcggaat gtgcctcaac tggggatgta
     4501 tcccctcaaa gtcaatgata gagagtgtaa aattttacca aaggattatc aaggattctt
     4561 cccgcttcgg cattgaagga attgaccgga aggtggtcac ctttagttgg aatacagcta
     4621 tcaaaagagc tgacacaatt gtaaagaggc tcacaggagg tgttgaattt cttcttaaga
     4681 aaaacggagt agagataatt acaggcgaag caagaattat ttcagcaaat tcagtacttg
     4741 tcgagaaccg cttgttgaaa acaaagaata taataattgc taccggttca acgtctctcc
     4801 cgatagataa cttatttgag aatcttgtta ttgagccgaa agatcttttc aaagaaagag
     4861 aaatccctga aaacatagtc attacaggaa atgcgccagt agcgatagag cttgcacaac
     4921 tattccggat gatggagaga tcagtcacat tagtgtctac tgccgatcac atcatgccca
     4981 aagccgatga ttatatcaga aaatatatgg agaataagtt gaagaaggat aaaattgaaa
     5041 tactctacaa cattggactt tcatcgactg caggatcttg gaatgacggc gttctcaacc
     5101 ttgaaggaaa agaggtcaag tgcgatctca ttataaacgc atcctctcgc aaaggaaacc
     5161 tcatcaaatc agacatagag attgtaattg aaaatgggtt ctactctgta aacgaagatt
     5221 gtgaaacatc tgtaaaaggg atttacgctg ttggggacgt aaatggagtc tcacaatttg
     5281 cgcatatagg ttcagcacag ggacttcatg ttgtcaatct tctcaaagga atccggcagc
     5341 gtcttgatat taagaaactc ccgatgaata tgtattctgt ccccgaagct gctcagatag
     5401 ggctcactga acaagaggca aaagaagagg gatacgatta caagatcagc gaatttcctc
     5461 tgtcagcaaa tggcaaggct atggccgaag gccaggcaga gggttttgta agacttattt
     5521 cagagaaaaa atacggagag gtacttggtg tacaaatcgt ggctccaaat gccacagaca
     5581 tgataaatga ggcagctgcc tatatgcaat tggaatcaac tgtgtacgac attgcacaga
     5641 ctgtccatac ccaccctact gtttcggaag tattcatgga agctggcttc gaggctgtag
     5701 acaaagcaat acataaataa attggaacca attgtaaata aatatactct gccagatgca
     5761 gcaattctcg actcacctga cgagtactca tacaaagtct ggcagccgga caagttatat
     5821 gctgtacttg gcagagccaa caacatagat gattcactca taaccggcaa tatcaccaag
     5881 gacaacgtta ccgtactaaa acgtccatct ggcggagagg cggtgatatt gtccccaagg
     5941 atgcttgtaa tatctgcaaa aatgaaattt tacaaagaga tgaatacaaa gcaaattttt
     6001 aaccgaatca acactttgct tatcgaccgc ctctccgcat taggaatcaa gggactttat
     6061 tcacgaggaa tctctgatct ctcgatcaat gacaggaaaa ttctcggttc ctcaatgtac
     6121 ctgaacaaag atgtaatttt ttaccatgcc gtgctgaata tatcagagga tgtatcactc
     6181 ttatcgaagt atctgaagca ccctacacgt gaaccagatt acagaaaagg gagaagccat
     6241 ctcgaattcg tcacctcaat tcataaagag ggatacccat tcacatcgcg cgagctatca
     6301 tctgtactca ccctcgcgct gcagcaaatc ctattttcaa aataaattct ataaaaaaga
     6361 tttatacaaa ttcataccca ttttacgaaa cataccggcg aaatttttaa tagcatttct
     6421 gtttggtttg tttattgtct ttttccttgt ggcattcaat ccatcacaca atacagactt
     6481 tgttcaagaa cagtttctga aagttactgt atatggattc ataacatttt tcactatcag
     6541 cttatacaat gtaggtgccc ctttgctttt cccaaaagta ttcgactcag aaaaatggag
     6601 tgtaaaaaac aatgattcgc ataagcctta aacaagtcga acaagccttc tcttattcag
     6661 agaatataat ttatcacata aagttccatc attaaagata accagctact ttcgtgaaaa
     6721 tttcaaaatt atgaataacg atacaaaaag gtacctgaaa tttcttatca cacccgttgc
     6781 aattctttta cttggaatcg ttatcggacg gtcttgtttc atatttttaa gaaactgggc
     6841 atggataccg gttgtattaa cttattggat agcaataggg ctggtcctat atttagatta
     6901 taagaagaaa gggactactc ctgtctattc ctgcaccaaa attgacgatg aaccgcaaat
     6961 tgcgggagta ttataatcat acaaccggag gtaactttat gtcagcggtt ttaaacgaca
     7021 aaggagaatt catcggtgtg aatttcgaca gaatatggca gggagtagta tcggattata
     7081 gatataattc aaccccatca caggctattg cggtggatat aagatatata atgtctctct
     7141 ttgataagtt gtaactggaa tagaggaggc aaacagataa tttaagctaa gtattcagga
     7201 ttaattacta tcttagaagt aattaaaatt caataaataa agttatgaat acttatgtag
     7261 ttattgcgat tatcgtgctg gtcatttttg cagcaggaat aaggattgta cggcctactc
     7321 accgcgccct gattgagagg ctcggtaaat attacaaatt tgccaatacg ggatttcact
     7381 ggataattcc tgtcatagac aaaatatata aggttaatgt aactgagagg atggttgatg
     7441 ccgagcctca ggagattata accaatgata atcttaatgc aaaggttgat gctcaggtat
     7501 attttcgtgt aaaatctgac gaggagagtg ttaagggatc tatttacaat gtcaataatt
     7561 ttcaatggca gatagtaaat cttgccagga ccacactcag aaatataatt ggcactctta
     7621 ccctgaagtc tgctaatagt gaaagaggaa agataaattc agatctttat aaaacattgc
     7681 atgacgagac aagagaatgg ggaattgaga tagtacgaac cgagctgaaa gagatagatc
     7741 ctccgaaaga tgttcaggag acaatgaaca aggttgtaaa ggcagaaaac gaaaagatag
     7801 ccgccataga ttctgctact gcagctgaga ctgtagctga cggtgtaaaa cgtgcaaaaa
     7861 taaaagaggc agagggatac aaacaatcaa agattcttca cgctgagggt gaggctgagg
     7921 caatacgcct tgtcaacgag gctgcagaca agtattttat cgggaatgca cagttgctaa
     7981 agagacttga gactctggaa ggggcacttt ctgaaaattc aaaaatagtt attccgacag
     8041 gaacagattt ggttaatatt atcagtgaca tggcaggagt gaatgtgctt ccattggaaa
     8101 aaagaaagaa gtctgaatag tctgatgata tgggcaatgc ttttttatta ttataacctt
     8161 attaatttaa taagattata ataataatta ttcagtaaac cctacattag aactgcttat
     8221 tgtattttaa aacaataaaa actcaatatt atgttaccaa ccatcaggaa agcaacagct
     8281 gacgacattt ctcaaatagt ctatttccag atgaatatgg cattagagac agagagactt
     8341 gagttaaaca gagatatact tacagctggt gtcacaaatg tactgacagg tgagaaggga
     8401 gcatcctatt acatggctga aatagatggc actttggccg gaatgctgat gatcactctg
     8461 gagtggagtg actggcgcaa tggatgggtg tggtggattc agtcggttta tatcagaccg
     8521 gaattcagag gtaaaggttt atacaaaatg ttatattttc atattgttaa ccttgcgaag
     8581 aaatcggatg atgtcatggg aatcaggtta tatgtagaca aaaccaatac taatgcacaa
     8641 aaggtgtata aagccctggg aatgaatggt gaccactacc tggtctatga atggatgaag
     8701 gattgactat tacatgtgat ttaattcttt ggatggaata aaaaaagaag ctgcctagta
     8761 ctttttagac agcctcttgt tttatgcatt ggttttttga cagattaata acgtcttctg
     8821 ttttcgtatc cgcctcttct atcgtttcca ccgccacggt tgtaaccgcc acggctgttg
     8881 tctctgttct cttttggttt agcaacattt acattgataa ctttgccgtc atactcgcat
     8941 ccgttaagtt cgttgattgc tttttgtcca gcttcttcgt cttgcatatc tacgaagccg
     9001 aatcctcttg atctgccggt ttctctgtcc atgataactt tagcagagct gatttcaccg
     9061 aactcgctga ataattggtt gagctcagcg tcttttacgc tgtaactcag atttgaaata
     9121 aaaatgttca ttaaataaac tttaatataa aataataata atgtgagtgt tatagatatt
     9181 aatgtctatt aataaactaa aaaatcagta aaaacataaa cttttgaatt gatgaataac
     9241 actctttcgg tgcaaagcta caataaaaaa atgatatttt aaacttggat ataaaaaaaa
     9301 tttctaaaat tgtaacatga gaaagaaaga tttaaagaaa cttatactgg ctctggtttt
     9361 aattactacc gtccagggaa tagggattga tatttatgta aactctgctg aaggtggaat
     9421 ctgggaggga ctttcaatat tcaaatactt cactttgcaa tcgaatgctc tggtcatgct
     9481 ttacgcgctc ctggcactca tcaatttcag atcaatgacg gaaaaagagt ggtttaaaag
     9541 agcccttaca cctgtcacct catatataat gcttacggga attacattcc tgattatatt
     9601 ggccccgact tcaaacgctc agggcctgca gagagttgca tctgatcttt tgcattatct
     9661 cgtaccatca ctgatgttct tctattggat tgtttttgaa gagagggaat tacagtatag
     9721 ttatatatgg agctggatta tatatccgtt tgtttttatg ttctggggct tataccttgc
     9781 aataatgaaa gaggactatc tctatccctt ctttgacata aacaaactcg gaatattgat
     9841 agtcccctat ctggccggca tgactattgc tgtttttctt atatgcagt
//
LOCUS       DAOTQL010000056         9674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6242_length_9674_cov_4.885331, whole genome shotgun sequence.
ACCESSION   DAOTQL010000056 DAOTQL010000000
VERSION     DAOTQL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6242_length_9674_cov_4.885331"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="P5293_04605"
     CDS             <1..642
                     /locus_tag="P5293_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRR49216.1"
                     /translation="GGRDVYLIYEPMAAAVGIGLDVEAPTGNMVVDIGGGTTEIAVIS
                     LGGIVVNYSIRVAGDGFTGEIQQYMRQQHSIKIGELTAEDIKIKVGAALSELDEYPEP
                     YVVRGPNLMTAHPVEVNVTSQEIAHCLDKSIAKIESAVISVLEQTPPELYADIVQRGI
                     FLTGGGALLRGLDKRLYEKINIPFHVSEDPLHAVAKGTSTALKNTDRYPFLMR"
     gene            649..1485
                     /gene="mreC"
                     /locus_tag="P5293_04610"
     CDS             649..1485
                     /gene="mreC"
                     /locus_tag="P5293_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRR49217.1"
                     /translation="MSRKTSARPVLISILAFIVLEGGAVWIMSKNSFFQRAKLNKIYT
                     EVEDKFLDINSDIRYYLTLNKSNEALAIENSYLRNSLAKYQSLFDTLSIRDSIAARLP
                     DSSLFSYIPARVISNSTNKSHNYLILNKGRKAGVKADMGVISANGVVGVVNAVSENYS
                     HVISLLNIKQSVSARIKGSDAFGPLMWNGVSVSHAVLTEIPQHIKFRIGDRVVTSGFS
                     AIFPPDIPLGKIVGSRIVFGTHHEIRVKFFQDFKTLKYVDIVVCKDRHEIDSLITAGN
                     EE"
     gene            1475..1993
                     /locus_tag="P5293_04615"
     CDS             1475..1993
                     /locus_tag="P5293_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49218.1"
                     /translation="MKSSLGTDIILFFSFLIAQILLSNFTDFGPMIFISIYPLYLLTR
                     PNNINPVISLISAFIMGLIIDYFTGDILGINAAAATMIAFVQPYLISIVLRKSLHEWP
                     IRPGMNELGFSKFCFYVFIGILLHHTAIILLENFSVHLDKYSLTRLFLSLALNLLLAI
                     LIEFGIFYKNWR"
     gene            2014..>3697
                     /locus_tag="P5293_04620"
     CDS             2014..>3697
                     /locus_tag="P5293_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRR49219.1"
                     /translation="MELKKSNILTLTVITIAVILIIRLFNLQIIDKEYKITASNNAFR
                     YEVRYPARGLILDRNGKILVGNETAYDIMVTPYEAVNFDTVALCHIFDLNVEDIRKTF
                     KEYRQNRRKIGFQTLPFVKRVSNRQYAVFLEQSYKFPGFSAVSRTLRTYPYNAGGNLL
                     GYITEVDTAYIRKHPEYKSGDYVGRTGIEQSYEEVLRGKKGYNIFLRDVHNKVKSSFA
                     NGEYDVDAIPGKNIISSIDAELQSYGEKLMTNKVGSLVAIEPSSGEILALISSPGIST
                     EKLASISKFYSEIVNDPLKPMFNRAIMAPYPPGSVFKLVNGLIALQDNVIDTIVKYPC
                     SMGFHIGRGVGCHAHPSPLDLTHAIMMSCNSYFCYAFRNIIDNPAYPDVTSAFSHWRD
                     MVESFGFGHKLGSDFPNELAGVLPSVKVYDRIHGKGKWKSLSIISLAIGQGEIGTTPL
                     HLANLAATIANRGYYYTPHIIKGAEDTVINTDYSKRHYTKVDTSNFEKIIYGMYLAVN
                     APPGSGGTATRVAVKGLDICGKTGTAQNPHGKDHSVFICFAPMNNPKIAVAAY"
     assembly_gap    3698..3797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3798..3998
                     /locus_tag="P5293_04625"
     CDS             <3798..3998
                     /locus_tag="P5293_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRR49220.1"
                     /translation="VFICFAPMNNPKIAVAAYIENAGFGATWAAPIASLLVEMYLDRK
                     VERAELESYVLNGNTMMNVPIK"
     gene            3995..5425
                     /gene="rodA"
                     /locus_tag="P5293_04630"
     CDS             3995..5425
                     /gene="rodA"
                     /locus_tag="P5293_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRR49221.1"
                     /translation="MIRESIYRKLDWQLIIIYLLLVLTGWLNIFSSVYDPELSKGILS
                     WDERSGMQFIWIIISIGVAALILFVISPKLYSVAAWLFYALGIIALLGVLVVGVDVNG
                     SKSWVAIGPFRFQPAEFSKIATALALSSLMGKYHFRLNSLKSKIKVILTIAVPMMLII
                     LEKETGSALVYLAFVFMLYREGLPGWYLLLGFMAIVLFVITLAFSPFEAIILLFSATV
                     IARGLFTHNILTHIIFLLLFMPLMIYVPNVGKLKIISSHILMADYYWLLLLISIPFIW
                     QIVKAVKTRINYHRNIVGAFIVSIALIFSVDFLFEKVLQEHQRARIENLLGITEDLQG
                     SGYNVHQSKIAIGSGGLFGKGFLQGTQTKFNFVPEQSTDFIFCTIGEEWGFAGSFLVI
                     ILYTILITRIINLSEKQKDSFNRIYGYSVASIIFFHFFINIGMTMGLVPVIGIPLPFL
                     SYGGSSLLSFTILLFIFIRLDLERWR"
     gene            complement(5397..5747)
                     /locus_tag="P5293_04635"
     CDS             complement(5397..5747)
                     /locus_tag="P5293_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRR49222.1"
                     /translation="MNDINNFESIQIDESGRVYKTLLTKKVKNRKVWKKSDIQEICSI
                     IPGTVAKLNVREGDKVKKGEEMLEYEAMKMLNIIKAPFDGIVEKIYVKEGDKLAKGDS
                     MLYIKSTSSVPDQV"
     gene            complement(5758..7308)
                     /locus_tag="P5293_04640"
     CDS             complement(5758..7308)
                     /locus_tag="P5293_04640"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRR49223.1"
                     /translation="MTSLSKKISELAERKSKATEGGGPSAIAKQIAMGKLPARERINQ
                     LLDEGSFFEYDLFIEHDARGFGLDGKTLAGDGVVIGTGTIHGRPVAIYAQDFTVAGGS
                     LGSMHAKKITKIMDYAMKMRIPLIGINDSGGARIQEGVNALAGYGEIFFRNTQASGVI
                     PQLSVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKSVLGEEIDMESLGGAR
                     VHSEISGNAHFFAYSETECFEQIKTLLDYIPANNSCKCARFEDFHEPKKSYKIEEIVP
                     ADPTKPYDVRDVIKALTDKSEFFEVQELWAANIVVGFGRIAGITVGFIANQPLVLAGV
                     LDCDSSDKSARFIRFCDAFNIPIVTIEDMPGYLPGIDQEHSGVIRHGAKLLYAYSEAT
                     VPKVTVILRKAYGGGYIAMNSRHLGADFVFAWPIAEIAVMGPDGAANIIFKKEIMDAE
                     NPEEMRRIKVEEYKQKFANPYVAAGKGYIDAVINPEETRKYLIHALEISQDKAVKGPE
                     KKHGIPPF"
     gene            7424..>9674
                     /gene="gcvP"
                     /locus_tag="P5293_04645"
     CDS             7424..>9674
                     /gene="gcvP"
                     /locus_tag="P5293_04645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRR49224.1"
                     /translation="MSLSFTDRHNGPREEEIKEMLAYIDANTFDELINQTIPSDIMLN
                     KPLDIDEGMTEQTYLSNLKAIASKNKNFRSFIGQGFYGTGTLPVIIRNVFENPSWYTS
                     YTPYQAEISQGRLEALLNFQTMISSLTGFKMSNCSMLDDATAAAEAVRMMYELRSREA
                     VKAGKNKVFLDEQTFLQVKAVIETRSSALGIEIVQGDFRTFKFDDKFCGAVVQYPAAN
                     GEVRDYTQFTSAAHEKGILVTAVADLLALAILKEPAAWGADIAVGSAQRFGLPMGFGG
                     PTAGFMATKDEFKRQMPGRIIGVSVDRLGNPALRMALQTREQHIKREKATSNICTATA
                     LMATMTGMYAVYHGPNGIREIAERIAAIAHTVASELIKSGIELSCNNFFDTIEIINTN
                     VEVVKERALECGFNFYYPDDKRVMISFDEITTVKEACDILEIFECNLDQTPKVVMYNG
                     FMRRESEILQESVFNKYHSETEMMRYIKKLEKRDISLTQSMIPLGSCTMKLNAAVEMM
                     PLSWSEFTNIHPFAPKDQVEGYLLLIKDLERDLATITGFDACSLQPNSGAAGEYAGLM
                     TIRAYHHAKGHKERNIAIIPTSAHGTNPASAAMAGMEILPVSCDENGNINVEELREKA
                     TANRDRLSCLMITYPSTHGVFEKRIREIISIIHENGGLVYMDGANMNAQVGITNPGFI
                     GADICHLNLHKTFAMPHGGGGPGVGPICASKELAPYLPSHPYLKNRNSQGVQAVSSSP
                     YGFPLLLPVT"
BASE COUNT         2939 a         1930 c         1844 g         2861 t
ORIGIN      
        1 ggtggccgag acgtctattt aatatacgaa ccaatggcag cagcagtagg gatagggctt
       61 gatgttgaag cccctactgg caatatggta gtagacatag gaggaggtac cacagagatc
      121 gcagttattt ctctgggagg tattgtcgtc aattacagca tcagggtagc cggggatggc
      181 ttcactggag aaatacagca gtatatgcgc cagcagcaca gcattaagat aggagaacta
      241 actgcagaag atataaagat aaaggtcggt gctgcactct cagagctgga cgaatatcct
      301 gagccatacg tggtgagagg tcctaatctt atgacagctc atccggttga ggttaatgtt
      361 acaagtcagg agattgccca ctgccttgat aaatctattg ccaagataga gtctgcagtt
      421 atctctgttc ttgaacagac acctcctgaa ctttatgccg acatcgttca aagaggtatt
      481 tttctcacag gaggaggagc attgctgaga ggcttggaca aaaggctcta tgaaaagata
      541 aatatacctt tccacgtttc agaagaccct ttgcatgcag tagcaaaagg aacaagtact
      601 gctcttaaaa atactgatag atacccattc ctaatgagat agatataaat gtcgaggaaa
      661 acctctgcca gaccggtact gatttcaatt ctcgccttca tagttcttga gggtggagct
      721 gtgtggataa tgtctaaaaa ttcatttttt cagagggcaa aactcaataa gatttatact
      781 gaagtcgaag ataaatttct tgatataaat tcagacatac gttattatct gacacttaac
      841 aaaagcaatg aggctctggc catagaaaac tcatatctta gaaattcact cgccaaatat
      901 caatcacttt tcgacacact cagtattagg gatagtattg cagccagact tccggacagt
      961 tccctttttt catatattcc ggccagggtt atcagtaaca gtacaaacaa aagtcataat
     1021 tatctaattc ttaacaaggg tagaaaggct ggagttaagg cagacatggg tgttatatca
     1081 gccaatggag ttgttggtgt ggtaaatgct gtaagtgaga attattctca cgtcatctca
     1141 cttctcaaca tcaagcagag cgtgagcgca agaattaaag gatctgacgc tttcggacca
     1201 ctaatgtgga atggagtaag cgtctcccat gcagtattga ccgagatacc tcaacacatt
     1261 aaatttagaa taggagacag agtagttaca agcggatttt ctgctatttt cccacctgac
     1321 atacctcttg gtaaaattgt aggttccagg atagtattcg gaacacatca tgaaataaga
     1381 gtgaaatttt ttcaggactt caaaactctg aaatatgtag atatagttgt atgcaaagac
     1441 agacatgaaa ttgacagttt aattacagca ggcaatgaag agtagcttag gtaccgatat
     1501 aatactgttt ttcagttttc tgattgcaca aattttattg agcaatttca ccgactttgg
     1561 tccaatgatt ttcatatcta tttaccctct ctatctgctt acaagaccaa ataatatcaa
     1621 tcccgtaatc tccctgattt cagcttttat tatgggatta attattgact acttcaccgg
     1681 agatatcctg ggaatcaatg cagctgctgc aactatgata gcctttgttc aaccctacct
     1741 catcagtatc gttctcagaa aaagtttgca tgaatggcct atccggccgg gaatgaatga
     1801 actcggtttc agtaagtttt gtttctatgt tttcattggc atattactac atcataccgc
     1861 aataatatta ctcgaaaatt tctcagtcca cctggacaaa tattctttaa caaggttgtt
     1921 tttaagtctt gcacttaatc tattgctggc aattttgatt gaatttggta ttttctacaa
     1981 aaactggcgc tgagtggagg attttctatg gcaatggaac ttaaaaagag caatattctc
     2041 acacttacag tcataacaat tgctgtaata ctaattatca ggctgtttaa tctccagata
     2101 attgataaag agtataaaat tacagccagt aacaatgctt tcagatatga ggttcgctat
     2161 cctgcccgag gacttattct cgacaggaac ggaaagatcc ttgttggtaa cgaaacagca
     2221 tatgacataa tggtaacacc ttatgaagca gtgaattttg atacagttgc cctgtgccat
     2281 atatttgatt taaatgttga agatatacga aagacattta aagaatatcg gcagaatcga
     2341 agaaaaattg gatttcagac tcttcctttt gttaagcgag tctctaacag acaatatgct
     2401 gtttttcttg aacagtcata caaattccca ggcttcagcg ccgtatcaag aacattaagg
     2461 acatatcctt ataatgcagg cggtaatctt ttgggatata tcacagaagt tgacactgcc
     2521 tatatccgta aacatcccga gtacaagagt ggagattatg tgggcagaac cgggattgaa
     2581 cagtcttatg aagaggtact cagaggtaaa aagggatata acattttttt gagagacgtt
     2641 cacaataagg ttaagtccag ctttgccaat ggtgagtatg atgttgatgc cattcctggg
     2701 aaaaatatta tatcttcaat tgacgccgaa cttcagagtt atggagaaaa acttatgacc
     2761 aacaaagttg gcagccttgt agccatcgaa ccttcatcgg gcgaaattct ggctctcata
     2821 tcaagcccgg gcatcagtac tgaaaaactt gcttcaatta gcaaatttta tagtgaaata
     2881 gtaaacgatc cattaaaacc gatgtttaac agagccatca tggcacctta tcctccgggt
     2941 tctgtcttca aactggtaaa tggccttatc gcactccagg ataatgtgat agatactatt
     3001 gtaaaatatc cgtgcagtat gggctttcac atcggaaggg gagttggctg tcacgcacac
     3061 ccttccccac ttgaccttac tcatgctatt atgatgtcct gtaactctta tttttgctat
     3121 gcttttagaa atattataga taatccagca tatcctgatg taacctctgc tttctcccac
     3181 tggagagata tggttgaaag tttcggattt ggtcacaaac ttggttctga ctttccaaac
     3241 gaactagccg gagtacttcc ttctgtcaag gtttatgaca gaattcacgg caagggaaag
     3301 tggaagtctc tatcaattat ttcacttgct atcggacaag gagaaattgg aactacaccg
     3361 cttcatcttg ccaatctggc tgcaactata gcaaatagag gatattatta tacaccccac
     3421 atcattaaag gcgctgaaga tacagttatt aacacagatt attccaagag acattatacc
     3481 aaagtagata cttcaaattt tgaaaaaata atatatggaa tgtatcttgc cgtaaatgct
     3541 cctccaggat caggaggaac tgccacaaga gtagctgtta agggattgga catttgcgga
     3601 aaaacaggaa cagctcaaaa tcctcatggt aaagatcatt cggtgtttat ctgcttcgca
     3661 ccaatgaaca atccaaaaat agccgttgca gcttatannn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnngtg tttatctgct tcgcaccaat gaacaatcca aaaatagccg
     3841 ttgcagctta tatagaaaat gcgggttttg gagcaacctg ggctgcgccg atagcttcac
     3901 tccttgttga aatgtatctt gacagaaagg ttgaaagagc agaactggag tcttacgtcc
     3961 ttaacggaaa tacgatgatg aatgtcccaa taaaatgata agagaaagta tatatagaaa
     4021 acttgactgg caattaatta taatttatct gctactggtt ctaactggtt ggttaaatat
     4081 attctcttct gtgtatgatc ctgaattatc taaaggaatt ctgagttggg atgaaagatc
     4141 cggaatgcag ttcatatgga taatcatttc aattggcgtt gctgcactta tcctttttgt
     4201 aattagccca aagctctaca gtgttgcagc gtggttgttt tacgcacttg gaataattgc
     4261 actacttggc gttcttgtcg ttggtgtaga tgtcaatggt tccaaatcgt gggtggcaat
     4321 aggacctttc cgattccaac ccgcagaatt ttcaaaaata gcaacagctc tggccctttc
     4381 atcccttatg ggaaaatacc attttcgact caattcttta aagtcaaaga taaaagttat
     4441 tttaaccata gctgtcccga tgatgttgat tattctggaa aaggagacgg ggtctgcttt
     4501 ggtgtattta gcctttgttt tcatgttgta ccgtgaaggg ttacctggct ggtatctact
     4561 gctgggattt atggcaatag ttctctttgt tattacactt gcattctctc cattcgaagc
     4621 aataatattg cttttttctg ctacagtcat tgccagaggc ctttttactc acaatatctt
     4681 aacccatatt atattcttgt tgctttttat gccattgatg atttatgttc ctaatgtcgg
     4741 caagctgaaa ataatttcat ctcatatact gatggcagat tactattggc tgcttctcct
     4801 catctccata ccttttatat ggcaaatagt caaggcggtc aaaacaagaa tcaattacca
     4861 tcgaaatatt gttggagctt ttattgtatc tattgctttg attttttcag tcgacttttt
     4921 gtttgaaaag gtacttcagg agcatcagag agcaagaatt gaaaatttac tcggaataac
     4981 tgaagatctg cagggatcag gttacaatgt tcatcaatca aaaattgcaa ttggctccgg
     5041 aggcctgttt ggtaagggat ttctgcaggg aacccaaact aaatttaatt tcgtaccgga
     5101 acagagtact gattttatct tctgcactat tggagaggag tggggatttg ccggttcttt
     5161 tcttgtaatt atactgtata cgatattaat aacacgtata atcaaccttt cagaaaagca
     5221 aaaagacagt ttcaacagaa tttatggata ctccgttgct tcgatcattt tctttcactt
     5281 ctttatcaat atagggatga cgatgggact tgtacctgta ataggaatcc ccctgccctt
     5341 tctgagctac gggggatcat cactactaag ttttacgatc ttactcttta tatttatcag
     5401 acttgatctg gaacgctgga ggtagattta atatagagca tactgtcacc cttggccagc
     5461 ttgtcacctt ccttaacata aattttttca actataccgt caaatggagc cttaataata
     5521 ttaagcattt tcatggcttc atattcaagc atttcttccc cctttttaac tttatcaccc
     5581 tctctgacat taagtttggc aacagtgcct ggaataattg aacaaatttc ctgtatatca
     5641 gactttttcc agactttgcg attcttcact ttctttgtaa gaagcgtctt gtaaaccctt
     5701 cctgattcat ctatctggat ggattcaaaa ttattaatat cattcatgtc tcaactatta
     5761 aaatggtgga ataccatgtt tcttttcagg gcctttgaca gccttgtctt gtgaaatctc
     5821 caaagcatga attaaatatt ttcttgtctc ctcaggattt ataactgcat caatataacc
     5881 cttcccagca gcaacataag gattcgcaaa cttttgctta tattcctcaa ctttaattct
     5941 tctcatctct tcaggatttt ctgcatccat tatctctttt ttgaaaataa tattggcagc
     6001 tccatccggt cccataacag caatttcggc aataggccat gcaaaaacaa aatctgcccc
     6061 gagatggcgt gaattcatgg caatataacc tcctccataa gcttttctga gaataacggt
     6121 aaccttggga acagtcgctt cactatatgc ataaagcagt ttggctccat gacgaataac
     6181 tccagaatgc tcctgatcaa taccaggcaa ataaccaggc atatcctcga tagtaacaat
     6241 agggatgttg aaagcatcac aaaatctgat gaatcttgca gacttgtctg acgaatcaca
     6301 atcgagtact cctgcaagca ccagaggttg attggcaata aaaccaacgg ttattcctgc
     6361 aattctccca aatccaacaa caatattggc tgcccacaac tcttggactt caaagaactc
     6421 agacttgtcg gttaaggcct ttataacatc tcttacatca taaggtttag ttggatctgc
     6481 aggaactatt tcttctattt tataactctt tttgggttca tgaaaatctt caaacctggc
     6541 acatttacat gaattatttg caggaatata atcaagaagt gtcttaatct gttcaaagca
     6601 ttcagtttca ctataagcaa aaaaatgggc gtttccggaa atctcgctgt gaactctggc
     6661 tccacccaaa gattccatgt caatttcttc tccaagtacc gatttaatta cttcaggccc
     6721 cgtaataaac atcttggata ttttatccac cacaaataca aaatcagtaa gtgcaggtga
     6781 atagacagcc cctcccgcac aaggcccgag aataacagac aattgaggaa ttacgccact
     6841 tgcttgagta tttctaaaga aaatctcacc ataaccggca agtgcgttga caccttcctg
     6901 tatccttgcc cctcctgaat cattaatacc aataagaggt atacgcatct tcatagcata
     6961 atccatgatc tttgtgatct tttttgcatg cattgaaccc aaagagcccc ctgcaacagt
     7021 aaaatcctgt gcatagattg caacaggtct accatggata gtcccggtcc cgatcactac
     7081 cccatcaccg gccaatgttt ttccgtcaag tccgaaaccc cttgcatcat gttcaatgaa
     7141 aagatcatac tcaaagaaag aaccctcgtc aagaagctgg ttaatccttt cccttgcagg
     7201 aagtttgccc atcgcaatct gctttgcaat agcagatggt cctccacctt ccgtggcctt
     7261 agacttgcgt tctgccagtt cagaaatttt tttactaagt gaagtcattt attttgaaat
     7321 tagtttcgca aaattatgaa aatattttta ttgccaaatt ttaagaattt ataattaaat
     7381 ttgtactttt aataataaat aataacttta taaatagcaa aatatgtcac tttcattcac
     7441 agacagacat aacggcccta gagaagagga aataaaagag atgcttgcat atatagatgc
     7501 gaacactttc gatgaactta taaatcagac aattccttca gacattatgc ttaataagcc
     7561 tctcgatata gatgaaggca tgacagaaca gacatatctt tcaaatctca aagctattgc
     7621 ttccaaaaac aaaaattttc gttccttcat tggtcaaggt ttttatggca caggaacttt
     7681 accagtcata atcagaaatg tttttgaaaa tcccagctgg tatacatcat atactccata
     7741 tcaggcagag atctcacagg gaagacttga agcacttcta aatttccaga ctatgatatc
     7801 aagtctcaca ggcttcaaga tgtcaaactg ttccatgctc gatgatgcaa ctgctgcagc
     7861 tgaggctgta agaatgatgt atgaactacg ctcaagagaa gccgtaaaag caggaaaaaa
     7921 caaagtgttt ctggatgaac agacattttt gcaagtcaaa gccgtaattg aaactcggtc
     7981 ctcagctcta ggaatagaga ttgtccaggg agattttcgc acatttaaat ttgatgacaa
     8041 gttctgtggc gctgtggtac agtatcctgc agctaacgga gaagtacgag attataccca
     8101 atttacatct gcggctcatg agaaaggtat tttagttaca gccgttgctg atttgcttgc
     8161 acttgcaatt ctcaaggagc cagcggcttg gggagcagat attgcagttg gttccgcaca
     8221 acgcttcgga cttccaatgg gttttggagg tcctacagcg ggttttatgg ctactaaaga
     8281 cgaatttaaa agacaaatgc caggaagaat cattggcgtt tccgtcgata gacttggtaa
     8341 tcctgcattg agaatggctc ttcaaacacg tgaacaacat atcaaaagag agaaagctac
     8401 ttccaatatc tgcacagcga cagctctgat ggccacaatg acaggcatgt atgcagtata
     8461 tcatgggcca aacggcattc gagaaattgc agaaagaatt gccgctattg cacatacagt
     8521 tgcttcggaa cttattaaat caggcattga actctcttgc aacaactttt tcgataccat
     8581 tgaaatcata aatacaaatg tggaagtcgt aaaagagcgt gccctggaat gcggcttcaa
     8641 tttctattat cctgatgaca agagagtcat gattagtttc gacgagatta cgactgtcaa
     8701 agaggcttgt gacatcctgg aaatctttga atgtaatttg gaccagactc ctaaagttgt
     8761 aatgtacaac ggctttatga gaagagaaag cgagatactc caagaaagtg ttttcaataa
     8821 atatcactct gagaccgaaa tgatgcgtta catcaagaaa ctggaaaaga gagatatttc
     8881 tctaactcaa tccatgattc ctctaggttc ctgcaccatg aaacttaatg cagccgtcga
     8941 gatgatgcct ttgagctgga gcgaatttac aaacatccat ccatttgccc ctaaagatca
     9001 ggttgaagga tatctgcttt taataaagga tttggagaga gatcttgcta caattaccgg
     9061 ttttgatgca tgttcattgc agcctaattc cggagcagca ggagaatacg ccggattgat
     9121 gacaataaga gcctaccatc atgcaaaagg ccacaaagag aggaacatag ccataatacc
     9181 tacatctgca catggtacaa atccggcttc agcagcaatg gccggtatgg aaattttacc
     9241 ggtttcgtgt gatgagaatg gaaatataaa tgttgaagaa ttaagagaaa aggcaacagc
     9301 aaatagagat cgcttatcct gccttatgat tacatatcct tccacacatg gtgtttttga
     9361 aaaacgaata agagagatta tctccatcat acacgaaaat ggaggacttg tttatatgga
     9421 tggagcaaat atgaatgctc aagtagggat aacaaatcct ggtttcatcg gtgccgatat
     9481 ttgtcatctc aacttacata agacatttgc aatgcctcat ggaggaggag gccctggagt
     9541 tggccctata tgtgcctcca aagaacttgc accatacctc ccttcccacc catatcttaa
     9601 aaatcgcaac tcacaaggag ttcaagcagt aagttcctct ccttacggtt tccctctttt
     9661 actccctgta actt
//
LOCUS       DAOTQL010000057         9612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6280_length_9612_cov_3.600502, whole genome shotgun sequence.
ACCESSION   DAOTQL010000057 DAOTQL010000000
VERSION     DAOTQL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6280_length_9612_cov_3.600502"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="P5293_04650"
     CDS             complement(<1..592)
                     /locus_tag="P5293_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49225.1"
                     /translation="MNKEHKNRLGLNKNIFYIGLTSFFTDTSTKMVYSVMPLFLLSIG
                     ASKSVISLIEGIAESTASLLKFISGFWSDKIGRSKPFMIIGYGVTAIVTPLYALAKIP
                     FHILALRFTERVGKGLRAAPRDKLIGDSVDKKDAGKNFGFHKAMDNSGAIIGPLLAAL
                     LLIAFPHNYRLIFIWAAIPAFIGVITIIFLIKDTRRP"
     gene            complement(585..2303)
                     /locus_tag="P5293_04655"
     CDS             complement(585..2303)
                     /locus_tag="P5293_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRR49226.1"
                     /translation="MNEKLFRKKSISHILNSSNADLSNPQLKRNLTVLDLTALGVAGI
                     VGAGIFSTIGNAAYSGGPAVSLLFVFTAIACGFSALCYAEFASRIPISGSAYTYAYAS
                     FGELVAWVIGWTLIMEYAVGNIAVAISWSDYFTGMMNGFGVKIPEFITTDYLSASRGF
                     REACVQMAAGTDFDSLTSALQESYMAWTKAPQIAGLRLIGDIPAFSIVVLITVLVYIG
                     IEETKIASNIMVLVKMIILLVVISVGAFYVNTDNWSPFAPNGLGGVLKGVSAVFFAYI
                     GFDAISTTAEECKNPQKDLPRAMFYSLIICVVLYVAISLVLTGMVSYKELAVGDPLAF
                     VFHKLNINWLSGIIAISAIFAMASVLLVFQVGQPRIWMSMSRDGLLPKRFGKVHKKFH
                     TPSFSTVITGLFVALPTLFLNLTEVTDLTSIGTLFAFIVVCAGILIVNRDEIQSEKGN
                     EKKGFRVPYYNSKLFLPPIVIIIIIALICFNLDGLKTFFSGYKATNIPMYGFLICASY
                     IVYISVKRSLSLIPVLGLLINMYLITELGIANWMRFLIWLIIGIIIYFTYGIKNSKLK
                     NYTVNE"
     gene            2386..>5446
                     /locus_tag="P5293_04660"
     CDS             2386..>5446
                     /locus_tag="P5293_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRR49227.1"
                     /translation="MKKFTIFFCLFLFFQILVNGEPVNTVATEARLLRFPDICGDQIV
                     FSCAGDLYIVSADGGVARKLTSNIGYEMFPRFSPDGKMIAFTAQYDGNTEVYVIPSTG
                     GEPRRLTYTATLVRDDIGDRMGPNNIVIDWTPDGKNILYRTRSYSFNDFTGQLMTVPV
                     DGGLSTKIPLKNGGFASFSPDGKKLAYNYVFREFRTWKRYKGGMADDIRIFDFSSKTS
                     EKITDNINQDIIPMWSADGKKIYYISDRDNYMNLYEYDIASKNTRQLTKYNEYDIKFP
                     AAGENAIVYENGGYIYKYSMVSAKAEKVPIYITNDITWSRPVWKDLSKSISSFSLSPN
                     GERILGVARGDIFSIPAKKGITYNLTNSCEANDKSADWAPDASGYAYISDKDGEFNIW
                     FRDIKSGKERKLTDIKGYIFGFEWSPDAKYIAWSEKKNTLNILEVATGKNYVVEESGV
                     GPINDYNWSPDSRYLAYTRPGKEMNTIVIYDVKDKGKFQITDEWYNSGSPNFSKEGKH
                     LLFVSARTFNPIYGQTEWNHVYTNMYKIYVIPLTKDAFIPFAPENDVIATEKADTDKA
                     PADKSAADKKSELQYNFDNISSRIVELPVEAGYYGNLHMIGKNIYYSSRKGGVMYDLE
                     TRKETDLNARLIFASGYKKVLAISGNSMQVMDIPKSSVSITSPVPIGGVKKLVDYHKE
                     WMQIYNETWRQMRDFFYASNMHGVDWNKIYKRYAELVPYVNHRSDLTYILGEMIGELN
                     VGHAYSQNGEHPEAPRISTGLLGAEFSKDKSGFFKVEKIIQGANWDKSTRSPFTMPGS
                     EVKEGEFILSINGQSVKDCQDIFELLQGTAGVVTEFEVNSVPDFAGSRKVLAEPLASE
                     ASLRYYNWVENNIRKVNEATNGEVGYIHIPDMGVPGLNEFVKHYYPQLSKKALIIDDR
                     GNGGGNVSPMILERLQRTITYFSMHTNQKEGDVNPVGTFQGPKVLLVNEYSASDGDIF
                     PYRFKYNKLGTVIGRRTWGGVVGYSGTVPVVDGGSIVTPSYAPFAADG"
     assembly_gap    5447..5546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5547..5786
                     /locus_tag="P5293_04665"
     CDS             <5547..5786
                     /locus_tag="P5293_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49228.1"
                     /translation="VDGGSIVTPSYAPFAADGSGWIIEGRGVEPDIEIFNDPYKEFMG
                     EDEQLEKAIEVIKKQMKEYNYKPATIPPFLDKSPK"
     gene            6145..7971
                     /gene="ilvD"
                     /locus_tag="P5293_04670"
     CDS             6145..7971
                     /gene="ilvD"
                     /locus_tag="P5293_04670"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRR49229.1"
                     /translation="MKNLRSTLSTTGRKMAGARSLWRANGMKEEQFGMPVIAVVNSFT
                     QFVPGHLHLHEVGQEIKQIIEAEGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMCNAHKVDGMICISNCDKITPGMVMAAMRLNIPSVFVSGGPMEAGRIDDKDYDL
                     IDVMVNGADVSYPEEKLAILEKNACPTCGCCSGMFTANSMNCLTEALGLALPGNGTLV
                     ATHSLRKDLFSKAAKLIAKNTKDYYFNSKDEILPRNIATKSAFMNAMALDIAMGGSTN
                     TVLHLLAIANEAEVDFKMEDIDSLSRIVPVICKVAPNSHYHMQDVHRAGGVMRILEEL
                     ASKGVLDLSVHRVDCATLGEAIENNSILNRQADKNVLNFYLAAPGMRHNYKLGSQNNY
                     YDKLDKDSVNGCIRDIKHAYLRDGGLAVLKGNLALNGCIVKTAGVDDSILQFRGRAIV
                     FESQEEASEGILSGNVHPGDVVVIRYEGPKGGPGMQEMLYPTSYLKSMKLDKECALIT
                     DGRFSGGTSGLSIGHVSPEAAAGGNIALIKDGDIIVIDISKRLVSVELSESEIEKRRH
                     EMLSNGNNAFTPRNRDRHISKSLKAYAMHVSSADRGGVRVID"
     gene            7985..>9612
                     /gene="ilvB"
                     /locus_tag="P5293_04675"
     CDS             7985..>9612
                     /gene="ilvB"
                     /locus_tag="P5293_04675"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRR49230.1"
                     /translation="MKNIVKGSEALLQTLIEEGVSTIFGYPGGTMIPLYDELYNFKGK
                     INHILVRHEQGAVHAAEGYARATGKVGVAIATSGPGATNLVTGIVDAYMDSTPLVCIT
                     AQVNLDKLGTDFFQEADIVGITIPATKWSYQITKASEIVGIVSKAFCIASSGRPGPVL
                     VSITKNALVDDVDYQYVRYTSSRLNKQFFKTKLEVEISEAVRMINNAERPMIIAGQGV
                     ILSGAERELLKLAERGNIPVVSTLMGLSAVSSNHPLFMGMVGMHGNVAPNKMTQQSDL
                     IVAVGMRFSDRVTGNTEKYAPNAKIIHIDIDKAEFNKNVKVDLPLWGDARGILGKIAP
                     EVKFNERKEWFSFISEYLEKERKMIINKLMKHDPNGMISMAQAVDCVARLSGGEAIIV
                     TDVGQNQMFGARYSKFNGKRSFITSGGLGTMGYGLPASIGAKMGVPDKEVVLITGDGG
                     FQMSIQELGTILQSQIKIKILLLNNSYLGMVRQWQQLFYDKRYSFTELINPDFMKIAQ
                     AYNIASRKIEHCSELEEGVTEMLSSEGSFLLEVVV"
BASE COUNT         2978 a         1622 c         2066 g         2846 t
ORIGIN      
        1 caggtcttct ggtatcctta atcaggaaaa taatggttat tactccaata aatgctggga
       61 tggctgccca tataaaaatt aagcggtaat tatgaggaaa ggcaatcaac agaagtgctg
      121 ccaaaagagg accgataatt gctccgctgt tgtccatggc tttatggaaa ccaaaattct
      181 taccggcatc ttttttatct acagaatctc caataagttt atctcgagga gcagcacgaa
      241 gcccttttcc aactctttca gtgaatctta gagcaagtat atgaaaggga atttttgcaa
      301 gagcatataa tggtgttact atggctgtta caccatatcc aattatcatg aatggtttgc
      361 tgcgcccaat cttgtcgctc cagaatcctg aaataaattt aaggagtgaa gcagtgcttt
      421 cagctattcc ctctatcaat gagataacac tctttgatgc ccctattgat agtaggaata
      481 atggcattac actgtatacc attttggtag aagtatcagt gaaaaaactt gtaagcccta
      541 tatagaatat atttttatta aggccaagtc tgtttttatg ctctttattc atttactgtg
      601 taatttttca acttgctgtt tttaatgcca tatgtaaagt aaataataat acctataatt
      661 aaccatatca agaatctcat ccagtttgca attccaagtt cagtaatgag atacatgttt
      721 atcagtaatc ccaataccgg aataagagaa agagacctct tcacggatat gtatactatg
      781 tacgatgcac agattagaaa gccgtacatg ggaatatttg ttgctttata accagaaaaa
      841 aatgttttaa gtccatcgag attgaagcaa ataagagcga ttattatgat aattacaatt
      901 ggtggaagaa atagtttact gttatagtaa gggactctga atcctttttt ttcatttcct
      961 ttttcggatt gaatctcatc tctgtttacg ataagtattc ctgcacaaac cacaataaat
     1021 gcgaaaagtg ttccgatgct tgtgagatcg gttacttctg taagattaag aaagagtgtc
     1081 ggcaatgcca caaataatcc tgttatgaca gtagaaaatg aaggagtatg aaatttttta
     1141 tgaacttttc caaacctttt aggaagcaat ccgtcacgac tcatgctcat ccatattctt
     1201 ggttgtccta cttgaaatac aagtaataca cttgccattg caaaaatggc actgattgct
     1261 attattcctg aaagccagtt aatattcagt ttgtgaaata caaatgcaag agggtcccca
     1321 acagcgagtt ctttgtaact taccatacca gtaagtacaa ggctgatagc cacatagagt
     1381 actacacata taatcaacga ataaaacatg gcacgaggaa ggtctttctg agggttttta
     1441 cactcttctg ctgttgtgga aatggcatcg aaacctatat atgcaaaaaa gacagccgaa
     1501 acacctttga gtactcctcc aagaccattt ggtgcaaagg gggaccagtt gtctgtattg
     1561 acgtagaatg ctccgacaga tatcacaacc agaagtatta tcatctttac cagaaccata
     1621 atgttacttg caatctttgt ctcttcaatg cctatgtata ccagcaccgt aatcaggact
     1681 acaatagaaa aggccggtat atctcctatt agacgtaatc ctgcaatttg aggagcttta
     1741 gtccatgcca tatatgattc ctgaagagct gaggtaagtg agtcgaaatc cgtgcctgca
     1801 gccatttgga cgcaggcttc acgaaaacct cttgaagctg aaagatagtc tgtagtaatg
     1861 aactcgggaa tttttactcc aaaaccattc atcatacctg tgaaatagtc tgaccatgaa
     1921 atagctactg caatattacc aactgcatat tccataataa gtgtccatcc tatcacccat
     1981 gccaccaact ctccaaaact tgcataagca tatgtatatg cacttccact tattggtatt
     2041 cttgatgcaa actcagcata acagagagct gaaaaaccac aagcaatggc tgtgaagacg
     2101 aaaagaagag aaacggctgg acctccgctg taagcggcat tgcctattgt gctgaagatc
     2161 cctgcgccta ctattccggc taccccgaga gccgtaagat ccaatacagt tagattcctt
     2221 tttaattgtg ggttgcttaa atcggcattt gagctattta gaatgtggct tatggatttt
     2281 ttcctgaaaa gcttttcatt catcagttat tgtttaagtt gccaaattta atgattcttg
     2341 ttaattatat tttttaaatt tgttacaata ttaaattaaa tgtttatgaa aaaattcacg
     2401 atctttttct gtctgttttt attttttcag attttagtca atggagaacc ggtcaatact
     2461 gttgcaacag aggcaaggct gctgcgtttt cccgatattt gcggagatca aattgttttt
     2521 tcttgtgcag gtgatcttta tattgtcagt gctgatggag gtgttgcaag aaaactgacc
     2581 tcaaacattg gttatgagat gtttcctcga ttttctcctg atggtaagat gatagcattt
     2641 acagcccagt acgatgggaa tactgaagtt tatgttattc cttctaccgg aggagagcca
     2701 agacgactta cttatactgc gactcttgta agagacgata taggagaccg tatgggacca
     2761 aataatattg taattgattg gacaccagat ggcaaaaata ttttatacag aacaagatct
     2821 tattcgttca atgattttac tggtcagttg atgacagtgc cggttgatgg aggactttcc
     2881 acaaaaattc ctcttaagaa tggtggcttt gcaagttttt ctcctgatgg caaaaagctg
     2941 gcatacaact atgttttcag agaattccgt acatggaaaa gatataaggg agggatggcc
     3001 gatgatatta gaatatttga cttctcatca aagacttcag agaaaattac ggataatatc
     3061 aatcaggata taatcccgat gtggtctgca gatgggaaga aaatatatta tatttcagac
     3121 agggataatt atatgaacct ttacgagtat gatattgctt ccaagaatac caggcaactt
     3181 actaaatata acgaatatga cataaaattc cctgcagcag gtgaaaatgc aattgtctac
     3241 gagaatgggg gatacattta taaatacagc atggtttccg ctaaggccga gaaagttccg
     3301 atttatatca cgaatgatat aacatggtcg cgtccggtat ggaaagatct ttccaaaagt
     3361 atatcatcat tttctctttc tccaaatgga gagagaattc ttggagttgc gaggggagac
     3421 attttttcta ttcctgcaaa aaagggtata acttacaatc tgaccaattc ctgtgaagct
     3481 aatgacaaat ctgcagattg ggctcctgat gcaagcggtt atgcttatat ttccgacaag
     3541 gacggcgagt tcaacatttg gttcagggat ataaaaagcg gcaaggaacg taaacttaca
     3601 gatataaaag ggtatatttt tggatttgag tggtctcctg atgccaagta tattgcatgg
     3661 agcgagaaga aaaatactct taatatcctt gaagttgcga caggcaaaaa ttatgttgtt
     3721 gaggagtctg gagttggacc aattaatgat tacaactggt ctcctgacag ccgctatctt
     3781 gcctatacaa gacctggaaa agagatgaat actattgtta tatatgatgt taaagataaa
     3841 ggtaaatttc agataacaga cgaatggtac aattctggct cgcctaattt cagtaaggag
     3901 ggtaaacatc tgctttttgt ttctgcccgc accttcaatc caatttatgg tcaaacagag
     3961 tggaatcatg tttacaccaa catgtataag atatatgtta ttccattgac aaaggatgct
     4021 tttattcctt ttgctcctga gaatgatgta atagctaccg aaaaagcaga tacagataaa
     4081 gcacctgccg ataagagtgc tgctgacaag aaaagtgaat tacagtataa ttttgataat
     4141 atttcttcaa gaatagttga acttcccgtg gaagcaggat attacggcaa tttgcatatg
     4201 atcggaaaga acatctatta ttcaagtaga aagggtgggg tgatgtatga cctggaaact
     4261 cgcaaggaga cagatttaaa tgcaagactg atttttgcct ctggttacaa aaaagttctt
     4321 gccatctcag gtaattcaat gcaggttatg gacataccga aaagtagtgt gtcaattact
     4381 tcgcctgtcc ctataggagg tgtcaaaaaa cttgtagact atcataagga atggatgcag
     4441 atttacaatg agacatggag gcaaatgaga gatttctttt atgcaagcaa catgcatggt
     4501 gtagattgga ataaaatata taagcgttat gcagaactgg taccttatgt aaatcatcgt
     4561 tctgatctta catatatttt aggggaaatg ataggagaat tgaatgtagg tcatgcatat
     4621 tcccaaaatg gagaacatcc tgaagctcca agaatttcaa caggtttgct gggtgcagag
     4681 ttttcaaagg ataagtcagg ctttttcaag gttgagaaaa taattcaggg tgccaattgg
     4741 gacaagtcaa ctcgttcacc ttttactatg cctggttcag aagttaagga gggtgagttt
     4801 atactgtcca taaacggtca atctgtaaaa gattgtcagg atatattcga attgcttcag
     4861 ggaacagcag gagttgtaac tgaatttgaa gttaattcag ttcctgattt tgccggttca
     4921 agaaaagttc tggccgaacc tcttgcaagt gaggcatctc ttcgttatta caactgggtt
     4981 gaaaataata ttcgtaaggt aaatgaagct acaaatggag aagttggata tatacacatt
     5041 ccggacatgg gagtgcctgg cttgaatgag tttgtaaaac actattatcc acagctctca
     5101 aaaaaggcat tgataattga tgatagaggt aatggaggcg gcaatgtctc tccgatgatt
     5161 ttagagagat tgcaacgtac tatcacctat ttctcaatgc atacaaacca gaaagaggga
     5221 gatgttaacc ctgtaggtac tttccagggt ccaaaggtat tgttggttaa tgagtattct
     5281 gcatcagatg gagatatctt cccatataga ttcaaatata ataaacttgg aactgttatc
     5341 ggccgcagaa catggggtgg agttgtagga tatagcggta cagttcctgt agtagatggt
     5401 ggatctattg ttacaccgtc atatgcccca tttgcagctg atggaannnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnngtag atggtggatc tattgttaca ccgtcatatg
     5581 ccccatttgc agctgatgga agtggctgga ttattgaagg cagaggggtt gaacctgata
     5641 tagagatatt taatgaccct tataaggagt ttatgggtga ggatgaacag cttgagaagg
     5701 cgatagaggt aattaagaag cagatgaagg agtataacta caaacctgca acaattcctc
     5761 catttctcga taaaagtcca aaataatttt taattggtat tgtcgaataa aattttttat
     5821 acatttgcat ttcaaagact gaattaccgg agataaactg atgaaccaat ttgccaacag
     5881 ttacttattc ttgattagtg ggcttttaca gagtcacact ccggtggttt ttgctaaaga
     5941 ggatctatct ctactatctt cagtcagtgg tgtcatatca gtgattacct ctgtcattat
     6001 tatttctgtg attattctag tcattagcac gggataggat cctttaaagt gtagaagata
     6061 tatttaaata taaatgaagc ttgctttaac gggatcctgt aaagcgagct tcattttttt
     6121 tttgcataat tcaaacatta cagtatgaaa aatttaagaa gcactctatc aacaacggga
     6181 agaaaaatgg ccggagcacg aagtctctgg cgggcaaacg gcatgaaaga agaacaattc
     6241 ggtatgccgg tgattgcagt tgttaattca ttcacacaat ttgtccccgg tcacctacat
     6301 cttcacgaag ttgggcagga gataaagcaa attattgaag ctgaaggctg ttttgctgca
     6361 gaattcaaca ctatagccat tgacgacggg atagcaatgg gacacgacgg catgctctat
     6421 tcgcttcctt cgagggatct tattgctgac agtgtggagt acatgtgtaa tgctcataag
     6481 gttgatggaa tgatctgtat atcaaactgt gacaaaataa ctcctggaat ggtaatggcc
     6541 gctatgaggt taaatattcc gtctgttttt gtttctggtg gtccaatgga ggcgggtaga
     6601 attgatgaca aagattatga tttaattgat gtgatggtta atggtgcgga tgtctcttat
     6661 cctgaagaga aattggcaat acttgaaaaa aatgcttgtc ctacttgtgg gtgttgttcg
     6721 ggcatgttta ctgccaattc tatgaattgt ctgactgagg ctctgggact ggcacttccc
     6781 ggtaacggaa cacttgtggc cactcattct ttgcgaaagg atctcttttc aaaggctgcc
     6841 aaacttatag ccaaaaacac gaaagactat tatttcaact ctaaagatga gatattgcca
     6901 agaaacattg caacaaagtc tgcctttatg aatgctatgg ctctcgatat tgcaatgggt
     6961 gggtcgacaa acactgtgct tcatcttctt gccatagcca atgaagccga agtagatttt
     7021 aaaatggaag atattgattc tctttcaagg attgtacctg ttatttgcaa agtagcacct
     7081 aacagtcatt atcatatgca ggatgtacac agagcagggg gtgtaatgag aattttagaa
     7141 gagttggcct ccaaaggtgt tttggattta tcagttcata gggtggactg tgcaactctt
     7201 ggagaagcaa ttgaaaataa cagcatcctt aatcggcaag ctgataagaa tgtgcttaat
     7261 ttctatttag cggctcccgg catgagacac aattataagt tgggttcaca aaataattat
     7321 tacgataaac ttgataagga ctcagttaat ggttgtataa gagatataaa acacgcttat
     7381 ttaagagacg gaggtctggc tgtcttgaag ggtaatcttg cattaaatgg atgtattgtt
     7441 aaaacggcag gtgtagatga ctctatttta cagtttagag gaagagcaat tgttttcgaa
     7501 tcacaggaag aggcctctga gggcattttg agtggcaacg ttcatcccgg tgatgtggtg
     7561 gttattcgct atgaaggtcc aaaaggaggg ccgggaatgc aagaaatgct ttaccctact
     7621 tcatatctta aatccatgaa actcgacaaa gagtgtgctc ttataactga cggcagattt
     7681 tcaggtggga cctccggtct ttctatagga catgtttcgc ctgaggcagc tgcaggagga
     7741 aatatcgcgc ttataaagga tggggatatt atagtcatag atatttctaa gagattggtt
     7801 tcagttgaac tttcagagtc agaaatagag aaaagacgtc acgaaatgct ctcaaatggt
     7861 aataatgcat ttactcctcg taatcgtgac aggcatataa gtaagtctct aaaagcttat
     7921 gccatgcatg tttcatcggc agatagaggc ggagtaagag taattgatta attgtaaagg
     7981 agctatgaaa aatattgtaa aagggtctga ggctttattg cagacactta ttgaagaggg
     8041 agtttctaca atttttggat atcccggggg tacaatgatt cctctttacg atgaacttta
     8101 taattttaag ggtaagatca accatatttt ggtaagacac gaacaaggag ctgttcatgc
     8161 tgctgagggt tatgcaagag caaccggcaa ggtgggagtt gcaatagcta cttctggtcc
     8221 aggagctacc aacctggtga caggaatagt agatgcctat atggattcaa cgcctcttgt
     8281 atgcattact gctcaggtca atctggataa attgggaaca gatttttttc aggaggcaga
     8341 tattgtcggc attaccattc ccgccactaa atggagttac caaataacta aggcttcaga
     8401 aattgtaggg attgtttcca aagctttttg tattgcatct tcaggcaggc cggggcctgt
     8461 acttgtaagt attacaaaaa atgcacttgt agatgatgtg gactatcaat atgttcgtta
     8521 cacctcttct cgtttaaata agcaattctt caaaacaaaa cttgaagtag agatttccga
     8581 agctgtcaga atgattaata acgctgagag gccgatgatt attgcaggtc agggcgttat
     8641 actttcaggg gcggagagag aactattgaa attggcggaa agaggtaata tccctgttgt
     8701 ctcaacactt atgggacttt cagctgtatc aagtaatcat cctcttttca tgggaatggt
     8761 gggtatgcac ggtaatgtgg cgccaaacaa aatgactcag cagagtgatc ttatagtggc
     8821 agtaggtatg cgtttcagtg acagagttac aggtaataca gagaaatatg ccccaaatgc
     8881 aaagattatt cacattgata tagacaaagc tgaattcaac aagaacgtca aagttgatct
     8941 tcccttgtgg ggtgatgcaa ggggaatttt agggaaaata gctccggaag ttaaattcaa
     9001 tgagagaaaa gagtggtttt cctttattag tgaatatttg gagaaagagc gtaaaatgat
     9061 aatcaataaa cttatgaagc acgatcctaa cggcatgata agcatggctc aagctgtaga
     9121 ttgcgttgcc agattgtctg ggggagaggc aattattgtg acagatgtag gtcaaaatca
     9181 aatgtttggt gcgaggtatt caaaattcaa tggcaaaaga agttttatta cctcaggagg
     9241 tttgggcaca atgggttatg gacttcctgc ctccattgga gcaaaaatgg gtgttcctga
     9301 caaggaagtt gtacttatta ccggtgatgg agggttccaa atgtctattc aggaacttgg
     9361 gactattctt caatctcaaa ttaagataaa aattttgttg ctaaataatt catatctcgg
     9421 aatggtaaga caatggcaac agctttttta cgacaaacga tattcgttta ccgaattgat
     9481 aaatcctgat ttcatgaaaa tagcccaggc atataacatc gcttcaagaa aaattgagca
     9541 ttgttcagag ctcgaagaag gagtaacgga gatgttgtca agcgaaggaa gttttcttct
     9601 tgaagtagta gt
//
LOCUS       DAOTQL010000058         9546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6331_length_9546_cov_4.578443, whole genome shotgun sequence.
ACCESSION   DAOTQL010000058 DAOTQL010000000
VERSION     DAOTQL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6331_length_9546_cov_4.578443"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="P5293_04680"
     CDS             complement(<1..1035)
                     /locus_tag="P5293_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR49231.1"
                     /translation="MNIKKTVLYILLSLFPLTLLSQEETVLRLKNHIEVLTSDSLMGR
                     MAGTPGEKAASIYIQKELSKLGIESMFGDEGEIFSFLSTFGDTISSQNVAGIIEGYDP
                     KLRDELIVVGAHYDHLGFNKLEINGREVTQIFRGANDNASGTAVMLEVARELVKVSFD
                     LRRSVIFIAFGASETSLTGSWYFLESGESFKDRIKLMINIDQVGTSEGEYHPIAYIYP
                     SINQLTDIVKNVSAKPVMILPEIRGNDTFSSDHINFVLQGIPVIMFTTALRKTLPSEK
                     EENEKLDLEGMASLTHYIAELVHTAANMDIIVSDTSAKQKESVEKPEKSEERVYTLYD
                     VDKKPSFLNGG"
     gene            complement(1044..1424)
                     /locus_tag="P5293_04685"
     CDS             complement(1044..1424)
                     /locus_tag="P5293_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRR49232.1"
                     /translation="MPKFRFVHNNINVRNLDISLDFYKEALGLNEVRRITPEDGSFII
                     VYLSDGESEHQLELTWLRDWNRPYNLGDNEFHLAFRTDDFNAAHQQHAQMGCICFENP
                     QMGIYFINDPDGYWIEVLPLRKHP"
     gene            complement(1535..2647)
                     /gene="gcvT"
                     /locus_tag="P5293_04690"
     CDS             complement(1535..2647)
                     /gene="gcvT"
                     /locus_tag="P5293_04690"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRR49233.1"
                     /translation="MKTTPFTQKHIALGAKMVPFAGYNMPLEYVGINEEHNHVRNSVG
                     VFDVSHMGEIWVKGPNALPFLQYTTSNDVSLLFPGKVQYSCFPNGKGGIVDDLLVYCI
                     DPLTYLLVVNASNIDKDYAHLSTIAPKFHITPGKELYNASDEICQLAVQGPFALKAMQ
                     KICDVNVFDMEYYTFRKLTIAGIKDAIFSITGYTGSGGCEIYVANEDADKLWNAVFEA
                     GKEFDIKPVGLGARDTLRLEMGFCLYGNDIDDTTSPIEAGLSWITKFNDSKGDFIDRD
                     YLLKQKNEGVKRKLIGFELIEKGVPRHGYSVCDANGTVIGVVTSGTVGPSVKKPIGMA
                     YVTPEFSKVGSEIYIKIREKLLKAVVVKTPFYKTAQ"
     gene            complement(2667..3143)
                     /locus_tag="P5293_04695"
     CDS             complement(2667..3143)
                     /locus_tag="P5293_04695"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRR49234.1"
                     /translation="MKKSAVVPLIIATLLLISGCDWIRGQLGMPTSSELDALRRSRNT
                     TQVQIDTQSKTFEKSGKTDSTLTSVENKDSVLHNRYYIVAGSFKDQNNARKMEDILRK
                     NGYTPVTLEMKNGFSMVTAGAFSSENEAMKEMNLLLEKEFSPGDLWIYDANTNKHK"
     gene            3232..4251
                     /locus_tag="P5293_04700"
     CDS             3232..4251
                     /locus_tag="P5293_04700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRR49235.1"
                     /translation="MRIFFLIYLQLFFSLPLNFRADTLKLLFLGDIMQHKAQLDAAHI
                     RGKDANDPLSYNYTSCFQYLKDDFNSADVIIANMETVFGPTPYSGYPVFCSPSSLLTA
                     ACDAGIDIFLTANNHICDKGKTGIEMTINLYDTLNVKFTGIYRNMEEKITRDPLIINQ
                     KSIKVALLNYTYGINGNNIPTPFVVNIIDTNQIKTDIARSKNLNPDFIIACLHWGVEY
                     SLKHNISQERVENFFFKNGVDIIIGSHPHVAQDISIYQLDNGDIKHITFYSLGNVISN
                     MTAPNTRNGLMAKILFYKGVNGEKKILPPECKTIFTERPGHKSANYSVIPGEEYKNRK
                     NITIK"
     gene            4248..5207
                     /locus_tag="P5293_04705"
     CDS             4248..5207
                     /locus_tag="P5293_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRR49236.1"
                     /translation="MTERIIILDEINPIEFYGVNNKLFELLCSYFPKLKVTARGSEIL
                     VKGSAEDTALFEEAVHKLIQIKQRKMHLNEADISSLFESDTVEGTMSQTDFSNIIVYG
                     TDGKIIRARTKNQIRLVEEYEKNDLIFAIGPAGTGKTYTAIALAVRALKNREVRRLVL
                     TRPAVEAGERLGFLPGDMKEKLDPYLQPLYDALRDMLPTKKLETLMEDGVIQIAPLAY
                     MRGRTLENAFVILDEAQNTTLGQLKMFLTRMGFDSKFIVTGDATQIDLPRRADSGLLK
                     GAGLCEHIKGVSIINFDRGDIVRHPLVSKIVDAFEKNETLRED"
     gene            complement(5210..6829)
                     /locus_tag="P5293_04710"
     CDS             complement(5210..6829)
                     /locus_tag="P5293_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRR49237.1"
                     /translation="MANTISRRSFLKSSVVAGAALGLGFYDSKKIGVKSQFDAIISNG
                     VIYVGDGSSPFKGSIGIKNGKIAAIGDIGDSADSIVNAEEMAVSPGFVDIHSHTDGNI
                     RYAKLGDSKIYQGITTDVSGNCGDSVFPLGGWKSISDFNNEMEKMGIGVNIRSFIGQG
                     TLRHVVIGDNNVPATKDHIKRMKYLLEEELEKGAIGLTCGLEYTPGSYASDDEIVELL
                     KVVAKHDKTFAIHMRNEDDRVEEAVAESIDMARRAKVRLQISHLKTQNVANWHKAPKL
                     IKQIEDARASGIDIAFDRYPYTAFSTGMSVFIPLDYRQGSTDEIIARLKDDKQSKEIA
                     KYLNNKLEKLGGAKNVMVTSARLAENRQFVGRNVEECAAMTNLSPWEFMRKILIDERL
                     VVDIVGFAMNEDNLKMFLSHPMGMPITDCSAYSPVGKLGENMPHPRAYGSFPRFVGKY
                     CRDENLMDLSSAVRKCTSLPASRIGLKERGLLKVGYYADILVFNPLTIIDKATYSEPH
                     QFAAGINHIFVNGVWTIANGVPTGKLGGRTS"
     gene            complement(6843..7853)
                     /locus_tag="P5293_04715"
     CDS             complement(6843..7853)
                     /locus_tag="P5293_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRR49238.1"
                     /translation="MNCSKRVNELVVFLLLAIVTTSCEKKLIYRTIDGFTQGTTYHIV
                     YSVDGADSLDEVVNEVLSAIDNSLSVYNDSSLISRINRGENIPPDAFFVELFNKSREI
                     YDLSSGAFDISGAPLFDIWGFGFKNREKVTQEDIDSIKTFVGMDKIWIDNGRVKRSDS
                     RVTLNMNAVAQGYTSDVMARKFDSLGVSNYMIEIGGEIFCKGHNPKGHEWNVGIDKPV
                     EGNFIPGEELQAVIALSGKGLATSGNYRKFYEEGGKKYSHTIDPRIGYPVTHSLLSAT
                     VIAQDAMTADALATFFMVVGLDEAIKFLNDRRDIDAYLIYSENGQFKVYKTAGVKIVK
                     IK"
     gene            7928..8947
                     /locus_tag="P5293_04720"
     CDS             7928..8947
                     /locus_tag="P5293_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HRR49239.1"
                     /translation="MRRFTILLLPIIAFMLLTISSCNKDDEDETTSEYMEGTLSFKCQ
                     EYLPVSTQIELNATGITNPTSGITYKWITVGFSADTTEGQNIIITTPSTTGDYTVTVS
                     ASADGFYSKSNSLAVKVIDITSENSLKGLKKSGEYITDPRDGKVYYYSTIGDLDWFNY
                     NLNYESAGKAYKDETLLAIFMGRLYTWSEATDNISKSGLGEGPRGVCPPGWSVPTAED
                     WENLAKVLNGYTTLPFHDSWRGLGEKLTVNAQLNDNNIWKYSPDNAQKNLFTWNALPA
                     GCMMNNLNRFENLFSFGFWWSSTEKDSNFAEYRYIYFDSPDFPYNYVTKEYFGASVRC
                     VRKSL"
     gene            8988..>9546
                     /locus_tag="P5293_04725"
     CDS             8988..>9546
                     /locus_tag="P5293_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRR49240.1"
                     /translation="MKLSKGTIVILVILAILLIFLFWGMRVYNKMVAQEEGVTSAWSQ
                     VENVYQRRADLIPNLVATVKGYATHERETLEGVVNARSKATQTTIDPKNMTPDAMEKF
                     QAAQGELTNALNKLMVVVERYPDLKANQNFLELQAQLEGTENRITVERQKFNDAARGF
                     NTYIRQFPKNIIASLFGFERKPYFEA"
BASE COUNT         3007 a         1985 c         1640 g         2914 t
ORIGIN      
        1 gccaccatta agaaatgatg gctttttgtc aacatcataa agtgtataaa ctctttcttc
       61 agatttctcg ggtttttcaa cactctcttt ttgttttgcg gatgtgtccg aaacaattat
      121 atccatatta gctgcagtat gtacaagctc ggcaatatag tgagtcaatg acgccatgcc
      181 ctctaaatcg agcttttcat tttcctcttt ttctgatggc agtgtttttc tcagcgcagt
      241 tgtaaacata atcaccggaa ttccttgtaa aacaaaattg atatggtcag aagagaaagt
      301 gtcatttcct cttatttccg gcaaaatcat aaccggcttg gcagaaacat ttttaacaat
      361 atcagtgagc tgattaatag aagggtatat ataagcaatc ggatggtact ctccttcaga
      421 agtgccaacc tggtcaatgt ttatcatcag cttaatcctg tctttgaaac tttcgccact
      481 ctccaggaaa taccaagatc ctgtaagtga ggtctcagaa gctccaaaag caatgaatat
      541 aacagaacgg cgcaaatcaa aggaaacttt aaccaactct cttgcaactt caagcatcac
      601 agctgttcct gatgcattat cattagcccc tcgaaaaatc tgagtcactt ctctgccgtt
      661 aatttcaagc ttattaaatc caaggtgatc gtaatgtgcg ccaacgacta ttaactcatc
      721 tctgagtttc gggtcatacc cctcaataat tcctgctaca ttttgtgaag aaatcgtatc
      781 cccaaatgtt gaaagaaaag agaaaatttc tccctcgtct ccaaacattg attctatccc
      841 caattttgag agttcttttt gaatatagat tgaagctgct ttctcgccag gagtcccggc
      901 cattctcccc ataagagaat cagaggttaa aacctcgata tgatttttaa gtctcaaaac
      961 agtctcttcc tgcgacaaaa gcgtcaaagg aaacaaagag agaagtatat aaagtacagt
     1021 ctttttaata ttcatgagtt ttcttaaggg tgctttctca atggtaatac ctcaatccaa
     1081 tatccgtcgg gatcatttat aaaataaata cccatttgag gattttcaaa acatatacaa
     1141 cccatttgag catgttgttg atgagcagca ttaaagtcat cagttctaaa tgcgagatga
     1201 aattcgttgt caccaagatt gtatggtcta ttccaatctc taagccatgt aagttcaagt
     1261 tggtgttcag attctccgtc cgaaagataa acaattataa aggatccatc ttcaggagtg
     1321 attcttctaa cctcattgag accaagagct tccttgtaaa aatcaaggct aatgtccaaa
     1381 tttctgacat taatgttatt atgaacaaat ctgaatttcg gcatgttttt gtctaattat
     1441 cttttgatcc attttgatag actacattgg cattgcaatc acagtaatag tacaatacaa
     1501 ctcttctgcc caattaaaat cgttcattat cggcttattg tgcagtctta taaaaaggtg
     1561 ttttcacaac cactgccttt aaaagctttt ctctaatctt aatataaatc tcactaccaa
     1621 ctttgctgaa ctccggagta acgtatgcca tgcctatcgg cttcttaaca gaaggtccta
     1681 ctgttcctga agtaactaca ccaattactg tgccatttgc atcacaaact gaataaccat
     1741 gtcttggcac tcctttttca ataagctcaa atcctatcag tttacgtttg actccctcgt
     1801 tcttctgctt aagcagataa tcacgatcta taaaatctcc ctttgaatca ttaaacttgg
     1861 ttatccaact caatccagct tctattggag atgttgtatc gtcaatatcg tttccataaa
     1921 gacagaaacc catctcaaga cgaagagtat ctcttgctcc aagccccacc ggcttaatgt
     1981 caaactcctt acccgcttca aaaacagcat tccacagttt gtcggcatct tcatttgcaa
     2041 cataaatctc acatccacct gaacctgtat aaccggttat ggagaagatc gcatccttta
     2101 taccggctat tgtgagtttt cggaaagtgt aatactccat atcgaaaaca ttgacgtcac
     2161 aaatcttctg catggctttt aatgcaaaag gaccctgaac agcaagttgg cagatctcgt
     2221 ctgaagcatt gtaaagttcc ttgcctggag taatgtgaaa tttcggagca atagttgaaa
     2281 gatgagcata atccttatct atattagatg catttactac cagtaaatat gtaagaggat
     2341 ctatgcaata taccagaaga tcatcaacga tgccaccctt gccgttagga aaacatgaat
     2401 actgaacctt gcccggaaac agcaaggaaa catcattgga agttgtgtac tgaaggaaag
     2461 gaagtgcatt agggcctttg acccaaattt ctcccatgtg tgatacatca aacactccga
     2521 ctgagttcct gacgtgatta tgttcttcat taatgccaac atactccagc ggcatattat
     2581 atcctgcaaa agggaccatc ttggcgccaa gagctatgtg tttctgtgta aatggtgtag
     2641 ttttcattat aaatagtgta caataattat ttgtgtttat ttgtattggc atcatatatc
     2701 cagaggtctc caggagaaaa ttctttttcc agcaataagt tcatctcttt catagcctca
     2761 ttttccgatg aaaaagctcc ggctgtaacc attgaaaagc cgtttttcat ttcgagggtt
     2821 acaggagtat atccgttctt cctgagaata tcttccatct tgcgggcatt attctgatcc
     2881 ttgaaacttc ctgcaacaat ataatatcta ttgtggagta ctgaatcttt attttcgaca
     2941 gaggttaaag tgctgtctgt tttgccagat ttttcaaatg ttttagactg tgtatctatc
     3001 tgcacctgtg tagtattcct acttcttctt aatgcatcaa gttcagaaga ggtaggcatt
     3061 ccgagctgtc ctcttatcca gtcacaccca ctgattaaca gcaaggtcgc aataattaac
     3121 ggaacaaccg cagacttctt cataatatgc taatgtataa gaaatttgta tattaacaaa
     3181 agtacatatt ttagcattaa gagaaaaata aatgtatatt tgaaataatt tatgagaatc
     3241 ttctttttga tatatctgca gctgtttttc tcattaccgc tgaatttcag ggcggataca
     3301 ttaaagttgc tttttttagg ggatatcatg cagcataaag cccaacttga tgctgcacat
     3361 atcaggggca aggatgctaa tgaccctcta tcctataact acacttcctg ttttcaatat
     3421 ctcaaggatg atttcaacag tgccgatgtc ataatagcaa acatggaaac agtattcggg
     3481 cctactccat actcaggtta tcctgtattt tgctctcctt cgtcactgct tacagcagca
     3541 tgtgatgccg gaattgatat ttttcttact gcaaataacc atatttgtga taagggtaaa
     3601 acaggtattg agatgactat caatctctat gatactctga acgtcaaatt tacaggcatt
     3661 tacagaaaca tggaagagaa aataacgaga gatcctctta ttattaatca aaagagtatt
     3721 aaagtagcac ttctgaatta tacttacgga atcaatggta acaatatccc gacacctttt
     3781 gttgtaaaca tcatcgacac aaatcagatc aaaacagata ttgccagaag taaaaatcta
     3841 aatcctgatt ttattattgc ttgtctgcat tggggagttg aatattcatt gaaacataat
     3901 atctctcaag aaagagttga gaattttttc ttcaaaaacg gagtagatat tataattggg
     3961 tcacatcctc atgtagcaca ggatatttca atatatcaac ttgacaatgg cgatattaag
     4021 catattactt tctattctct tggaaatgtc atatcaaaca tgacggcacc aaatacacga
     4081 aatggtctga tggccaaaat attattctat aaaggagtaa atggagagaa aaaaattctt
     4141 ccccccgaat gtaaaactat ttttactgaa cgacccggtc ataaatccgc taattattcg
     4201 gtcatccctg gggaagaata taaaaacaga aaaaatatca ctataaaatg acagaaagaa
     4261 taatcattct ggatgaaatt aatccgattg aattttacgg ggtgaacaat aagctttttg
     4321 agctgctttg cagctatttt ccaaaactta aagtaactgc aagaggatcc gaaatactgg
     4381 ttaaaggcag tgctgaagat actgccttat ttgaagaggc tgttcacaaa ctaatccaaa
     4441 taaagcaacg gaaaatgcac cttaatgagg cagatatttc atctctgttt gagtctgata
     4501 cagttgaagg caccatgtca caaacagatt ttagcaatat aatcgtttat gggacagacg
     4561 gcaaaattat cagggcaagg actaaaaatc agattcggct agtagaagaa tatgaaaaaa
     4621 atgatcttat ttttgctata ggtcctgccg gaacaggaaa aacatataca gcaattgctc
     4681 ttgccgtaag agcactcaaa aatcgtgaag tgagaagatt ggtgcttaca aggcctgctg
     4741 ttgaagcagg tgaaagactc ggttttcttc ccggtgatat gaaggagaag cttgacccct
     4801 atttacagcc gctttatgat gctttaagag acatgcttcc cacaaaaaaa cttgaaactc
     4861 tcatggagga tggtgttata caaatagctc ctctcgcata tatgagagga agaactcttg
     4921 aaaatgcatt cgtaatactt gacgaagcac agaatacaac tttaggacag cttaaaatgt
     4981 tccttacaag aatgggcttt gattcaaaat ttatcgttac cggcgatgcc acacaaattg
     5041 accttccaag acgagccgac tcaggattac ttaaaggtgc cgggctgtgt gaacatatta
     5101 aaggcgtgtc aataattaac ttcgatagag gagatattgt caggcatccc ctcgtttcaa
     5161 aaattgttga tgcttttgaa aaaaatgaaa ctctaagaga agattgagat taacttgttc
     5221 ttccgccaag tttccctgta ggtactccat tggctattgt ccaaactccg ttgacaaaaa
     5281 tgtgattaat gcctgcagca aattggtgag gctctgaata agtggcctta tcgatgattg
     5341 tcaggggatt gaataccaaa atgtctgcat aatagccaac ctttagcagg cctctctcct
     5401 ttaatcctat tctcgacgca ggaagagatg tacattttct gacagctgaa cttaaatcca
     5461 taagattttc gtctcgacaa tacttgccaa caaatcttgg aaaagaacca tatgctctgg
     5521 gatgaggcat attctctcca agtttcccca caggagaata agcactgcag tctgtaatag
     5581 gcattcccat aggatgggat aaaaacatct ttaaattgtc ttcgttcatc gcaaacccaa
     5641 ctatgtccac gacaaggcgc tcatctataa gtatttttcg cataaactcc catggagaaa
     5701 gatttgtcat ggcagcgcat tcttcgacat tacgtccaac aaattgtcta ttctccgcca
     5761 aacgtgctga cgttaccata acattcttag ctccgcccag tttttcaagt ttattgttta
     5821 aatattttgc aatttcttta gactgcttat cgtcttttaa acgggcaatt atctcatctg
     5881 tagagccctg acgataatcc aaaggaataa aaacagacat tccggttgaa aaagccgtat
     5941 atggataacg gtcaaaggct atatctattc ctgatgctct tgcatcttct atttgtttaa
     6001 taagtttggg cgctttgtgc cagttagcaa cattttgagt cttaagatgc gatatctgta
     6061 atcttacttt tgcacgtctt gccatgtcaa tgctttctgc aacagcttct tcaactctgt
     6121 catcttcatt gcgcatatga attgcaaatg ttttgtcgtg tttagcaacc actttcaaca
     6181 actctacgat ttcatcgtcc gaagcatatg aaccgggtgt atattcaagc ccgcatgtca
     6241 atcctattgc tcctttttca agttcttcct cgagaagata tttcatcctc ttaatatgat
     6301 ctttagtggc cggaacatta ttatctccta taacaacatg tctcaacgtg ccctgtccta
     6361 tgaaacttct tatgttaacc cctattccca tcttttccat ctcattattg aaatcagaaa
     6421 tgcttttcca ccccccaaga gggaaaacgc tatctccgca gtttccactt acatcggtag
     6481 taatcccttg ataaatttta ctgtctccta atttcgcata tctgatattc ccatcagtat
     6541 gagagtgaat atctacaaac cccggagaca cagccatttc ttcggcatta acaatactgt
     6601 ctgcactgtc cccaatatct ccgatagccg cgatttttcc atttttaatt cctatagagc
     6661 ctttaaatgg agaagaacca tcgcccacat agataactcc attggaaata atcgcatcaa
     6721 actgactttt gactccaatc tttttggaat cgtaaaagcc taatcccaaa gcagctcctg
     6781 ctactacaga actcttcaga aaacttcttc gtgaaatagt atttgccata acagcctcac
     6841 tcttatttta ttttaacaat tttaacccca gcagtcttat ataccttgaa ttgaccattt
     6901 tccgaataaa tcagataagc gtcaatatcg cgcctgtcat taagaaattt tattgcctca
     6961 tcaaggccta caaccataaa aaaagttgcc aaagcatcag cagtcattgc atcttgggca
     7021 attaccgtag cacttagcaa tgaatgtgta acaggatacc caattcgagg atcaattgta
     7081 tgagaatatt ttttaccacc ttcttcataa aattttctat aattgcctga agttgcaaga
     7141 cctttcccag aaagtgcaat tactgcctgc aactcctctc cgggaataaa attaccctct
     7201 acaggtttgt caatccccac attccattca tgccccttag gattatgacc cttgcagaag
     7261 atctctcccc ctatctcaat catatagttg cttactccca aactgtcaaa cttacgcgcc
     7321 ataacatctg aggtatatcc ctgagctaca gcattcatat taagagtaac tctcgaatca
     7381 cttcttttca ccctgccatt atctatccag attttgtcca ttcctacaaa ggttttaata
     7441 ctatcaatat cctcctgagt cactttttct ctgttcttaa agccaaaacc ccaaatatca
     7501 aagagaggag ctccagaaat atcaaacgct cccgaactta aatcgtaaat ctccctcgat
     7561 ttattgaata gttcaacaaa aaacgcatcg gggggaatat tttcccccct gttgatcctt
     7621 gagatcaaag aagaatcatt gtaaacagag agtgaattgt caattgcact taaaacttcg
     7681 ttgaccactt cgtccaaaga atccgccccg tcaactgagt atactatatg gtatgttgtc
     7741 ccttgagtga agccgtcaat tgtcctgtaa attaatttct tctcacaact tgtcgtaaca
     7801 atagccaaca acagaaatac aactaattca ttaactcttt tagaacaatt cataataaga
     7861 accattttgg cacaaattta gaataaataa aattatattt gtacgaaata aagactcttt
     7921 caatcatatg agacgattca caattctcct tttgccaatt atagctttca tgcttctaac
     7981 catttcatcc tgtaacaaag acgatgaaga cgaaactaca tccgaatata tggaaggtac
     8041 actttctttc aaatgtcaag agtacctgcc tgtttctacc cagattgagc ttaatgcaac
     8101 cgggataact aatcccacat caggtataac atacaaatgg ataacagttg gattcagtgc
     8161 tgacacaact gaaggtcaga atattataat tacaacacca tctacgacag gtgattatac
     8221 tgtaaccgtt tctgcatctg cagatggttt ttattccaag tcaaactcac tggctgttaa
     8281 ggttattgat attacttccg aaaacagtct caaaggattg aaaaaaagcg gagaatatat
     8341 aactgatcca agagatggta aagtttatta ctattcaacc ataggtgacc ttgactggtt
     8401 caactacaac ttaaattatg aaagtgccgg taaggcatac aaagatgaaa cacttcttgc
     8461 catatttatg ggcagacttt acacatggtc tgaggcaacc gacaacatca gtaaaagcgg
     8521 actgggcgaa ggacctcgcg gtgtgtgtcc tcccggctgg tctgttccta cagcagaaga
     8581 ctgggagaat ttggcaaagg tacttaatgg atacaccact cttccttttc acgattcttg
     8641 gagaggtctg ggagaaaaat taactgtcaa cgcacagcta aacgataaca atatatggaa
     8701 atacagcccg gataatgccc aaaaaaatct ttttacctgg aatgcacttc cagcagggtg
     8761 tatgatgaat aatttaaatc gttttgaaaa tttgttttct ttcgggttct ggtggtcatc
     8821 aaccgaaaaa gattcaaatt ttgctgaata tcgttacatc tattttgaca gtcctgattt
     8881 tccatataat tatgtgacaa aagagtattt tggtgcatct gttagatgtg tcagaaaaag
     8941 tctctaactt gtttaaatca ttattaataa attaattaga ttaaattatg aaattatcaa
     9001 aaggaacaat tgtaattttg gtgatattag caattctgct catttttctc ttttggggaa
     9061 tgcgagttta taacaaaatg gttgcccaag aagagggagt aacatctgca tggtctcagg
     9121 ttgaaaatgt ttaccagcgt cgtgctgacc ttattccaaa tttagtggct actgtcaaag
     9181 gttatgccac acatgaaagg gaaactcttg aaggtgtagt caatgcaaga tccaaagcta
     9241 cgcagaccac aatagaccca aaaaacatga ctccagacgc catggaaaaa tttcaggcag
     9301 ctcagggtga actgacaaat gcactgaaca aattgatggt tgtagttgaa cgttatcctg
     9361 atttaaaggc aaaccaaaac tttttggaat tacaagcaca acttgaagga actgaaaaca
     9421 ggattacagt agaaagacag aagttcaatg acgctgcacg agggtttaat acctatatcc
     9481 gtcaatttcc aaaaaatatt atagcttctc tctttggttt cgagagaaaa ccatatttcg
     9541 aagcaa
//
LOCUS       DAOTQL010000059         9537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6335_length_9537_cov_5.557055, whole genome shotgun sequence.
ACCESSION   DAOTQL010000059 DAOTQL010000000
VERSION     DAOTQL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_9537_cov_5.557055"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..652)
                     /locus_tag="P5293_04730"
                     /pseudo
     CDS             complement(149..652)
                     /locus_tag="P5293_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035718769.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(709..831)
                     /locus_tag="P5293_04735"
                     /pseudo
     CDS             complement(709..831)
                     /locus_tag="P5293_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
     assembly_gap    968..977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1062..2099)
                     /gene="galE"
                     /locus_tag="P5293_04740"
     CDS             complement(1062..2099)
                     /gene="galE"
                     /locus_tag="P5293_04740"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRR49241.1"
                     /translation="MHKQKILVTGGTGYIGAHTVVELYESGYEAVIVDNFSNSSPDVL
                     LGIEQITGKPPIFFEADCSDYHSFANVFKTFPDIRAAIHFAAFKAVGESVEKPVEYYK
                     NNINSLINLIDLMRGREDSSIVFSSSCTVYGQPDHLPVSENAPLQPATSPYGNTKQIC
                     EEILRDCALSLKGKLKVVSLRYFNPIGAHPSSLIGELPRGVPQNLLPFITQTAAGVRK
                     ELSVFGDDYNTPDGSCIRDYIYVCDLAKAHILALDRLISGRSEMEGDMEYYNLGTGQG
                     ISVLQMVSLFEKATGIKIPYKITERRAGDIEKVWADPSKANNMLGWKAVTPIDDVLRS
                     AWKWEKKMRGI"
     gene            complement(2118..3242)
                     /locus_tag="P5293_04745"
     CDS             complement(2118..3242)
                     /locus_tag="P5293_04745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRR49242.1"
                     /translation="MTIPMLDLKGQYKKIKKDVDESIQKVIDSAIFINGKEVFNFEKN
                     LSEYLLSKHVIACANGTDALQIAMMALELKPGDEVIVPAFTYAATAEVVALLGLVPVL
                     VDVYSDTFNIKVEDIEPSVTPKTRAIVPVHLFGQTSDMGRILEIAQKYNLYVIEDNAQ
                     ALGAKYRFEDNSEKFAGTIGHIGCTSFFPTKNLGCYGDGGAIFTQDSSLAERIRMVTD
                     HGQKVKYYHDIIGCNSRLDTIQAAILNVKLDHLNEYSASRYQAAQRYKELLSDVRELV
                     LPVESDFSTHVYHQFTLRVKEGRNELKKFLNEKGIASTIYYPLPLNRQKAFNRISKIY
                     VSLENSGRVANEVLSLPMHTELTEDIQMEVCNAIKDFFGY"
     assembly_gap    3283..3292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3319..3765
                     /gene="rpiB"
                     /locus_tag="P5293_04750"
     CDS             3319..3765
                     /gene="rpiB"
                     /locus_tag="P5293_04750"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRR49243.1"
                     /translation="MLTNDKIIGIAADHAGFELKEILKKEIESMGFTCKDFGTYSTES
                     IDYPDVAHPLAEAVSKGEITRAVAICGTGNGISMVLNKHAGIRAALCWNEEIASLARQ
                     HNNANICSLPARFISSSLAISIVKTFLTTEFEGGRHKRRIDKIDIK"
     gene            3765..4505
                     /gene="truB"
                     /locus_tag="P5293_04755"
     CDS             3765..4505
                     /gene="truB"
                     /locus_tag="P5293_04755"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRR49244.1"
                     /translation="MPFYILDREHVNAKLSTDFNNYPSGIVIPLDKPYKWTSADAVRK
                     IKFMLQNFFKIRNIKVGHAGTLDPLATGILLICVGNATKEAEKLQQEEKEYLAEITFG
                     CTTPSFDKEKEIDAYFPYEHIDKPLIETSLTKFIGEQDQIPPLFSAKLVDGKRAYEIA
                     RSGNSMELKPSKITISELNIVTYEKPLLVLQIRCSKGTYIRSLARDLGYSLDSGAYLS
                     GLVRTSTGIFNLKNSVKIEEIESLLKQI"
     gene            4555..5604
                     /gene="queA"
                     /locus_tag="P5293_04760"
     CDS             4555..5604
                     /gene="queA"
                     /locus_tag="P5293_04760"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRR49245.1"
                     /translation="MKLSQFNYPLNHNQIAKYPAKYRDESRLMILHKSTGEIEHRIFK
                     DILDYSEEGDIYIFNNTKVFPARLKGNKEKTGAEIEVFLLRELNREQKLWDVLVDPAR
                     KIRIGNKLYFGENDNLVAEVIDNTTSRGRTLRFLFDGPHEEFKEALFKLGEMPIPKWI
                     RTKVESIDYERYQTIFAQKEGAVAAPAAGLHFSRELLKRMEIKGVNSTFITLHMGLGN
                     FRTVDVEDLSKHKMDSEQVEITEESANSINETKDKGKKVFAIGVTVLRAVETSVTTQG
                     RLKPFEGWTNKFIFPPYNISVPDALVTNFQLPQSTMLMAVAAFGGYDPVMNAYHVALK
                     EGYKFGTYGDAMLIL"
     gene            5693..6808
                     /gene="dprA"
                     /locus_tag="P5293_04765"
     CDS             5693..6808
                     /gene="dprA"
                     /locus_tag="P5293_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRR49246.1"
                     /translation="MQNDELLCTVAINNIFCYRPKLSNTILEEFGSFSPLFRMSYSEL
                     VERFGKNYDFLSKISNGKNLEYAEEEIAWAKNEGIKIIPISSEDYPSKLKGCSDPPIL
                     LYLLGNAPLNNNLLISIVGTRMPTKYGSECCKNIIQTMKEIGLKPVIVSGLAYGIDAV
                     AHTAALNASLSTIAILPGGMEKIYPASHKNLAEKIINNGSLITEFPRNSQFHKVNFVQ
                     RNRIIAGLSEATIIIESRERGGALITAKLAHSYSREVFALPGRIGDICSQGCNTLISK
                     NLASIFTSTEDFVKSMGWELKRSHQPVQKKLFYYDDPNKEKIILALNNESGLTKDEIM
                     AKCHINLPLLSSLLLDMELEGSVLALPGERYSIITHR"
     gene            6805..7584
                     /locus_tag="P5293_04770"
     CDS             6805..7584
                     /locus_tag="P5293_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRR49247.1"
                     /translation="MKKYIDTHSHLYDADFVTDIDEVISRAEEEGVFSCILPGIDKKS
                     HSDLIALSNKKSSFAYPATGLHPTSVNNEWEDELAFVFDNAKKGIYTAIGEIGMDGYW
                     SKEFLEQQFIAFQKQLELAAMLSLPVIIHVREATEKTFSILDKTKHLGLKGVFHAFSG
                     SYETFKRTQKYGDFMVGIGGTVTYKNSSLPSTLLKIPLDRIVLETDAPWLTPVPFRGK
                     RNEPSFIPFIADKIAESKRCSIDEVAEITTANAIRLFKLQK"
     gene            7572..8615
                     /locus_tag="P5293_04775"
     CDS             7572..8615
                     /locus_tag="P5293_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRR49248.1"
                     /translation="MTKMNSSILIIYTGGTIGMKQDPDTLALVPFKFDQIEAEVPELR
                     KFGYKIDSYSFDPPIDSSEINTSFWVELAEIIYENYEKYDGFVILHGTDTMSYSASAL
                     SFMLENLEKPVIFTGSQLPIGMLRTDGKENLISSIEIAAARDDNGRAIVPEVCVYFES
                     RLYRGNRTTKDNAENFRAFRSANYPYLAEVGIHIKYNRNFISYPEKWGQQFKINTSLD
                     TNVAILKIFPGISQYVVESFLRINGLRAIILETYGSGNAPTFDWLISLLKNSINQGLI
                     ILNVTQCPAGSVNMEAYSTGILLKKIGVQSGYDSTTEAALTKLFFILGHNFDNQNIVN
                     LLSKNLRGEISID"
     gene            8719..9504
                     /locus_tag="P5293_04780"
     CDS             8719..9504
                     /locus_tag="P5293_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRR49249.1"
                     /translation="MKKFFMFLAVVGLIAISAQYVSAQTPDSAATTTSVAAASTAAPS
                     SDQTVQKEIPIHQQLKTKFIEGGPGFMAAILLCLIIGLAIAIERIIYLNLASTNIEKL
                     LKQIEDALASGGVEAAKEVCRNTKGPVASIFYEGLSRADEGIEMVEKSISSSGSIQMS
                     QLESGLNWINLFISIAPMLGFLGTVIGLVMAFDDIQKAGDISPNIVAGGMKVALITTI
                     GGLIVAIILQLFYSYIVSKIDSIVLSMEDSSVSLIDILVKSKK"
BASE COUNT         3064 a         1895 c         1713 g         2845 t
ORIGIN      
        1 ggaccccaaa actgtcatat gttcgttgcg atgttacaca actttcttca tcctcaaaac
       61 cgaatgagat tttatcatca tccttgccac ggcaattcct tcgataaaag tttgcatggg
      121 agtttttccg aagcagtatc cacgcatcaa tgtcagcagc aacaagtgca ttctttctgt
      181 catacaactt tgcaaaccca attttagagt aagtgtcgat gaccgtctgc tgataaatat
      241 gtcccacacc tttgatatgt cccacataat aagtgtcttg ggcaacaagg aaaccggggt
      301 aataagtctc tatttcccca tgggcatact tctcctcttt cgctttctcc aatgctgcta
      361 cctggttctc atcgaggatt atgccctctt gttccacttt gactgataac gctttcaggc
      421 gtttctggaa ggtctccatg tcatgcctta accatatgga acgcactcca ccaggtgaaa
      481 taagaatacc tttcttacgc agttcattgg atacccggac ttgtcccaat gcaggattgt
      541 ctgtcgccat ttgtaagaca gcctgttcga tgtgtcctta cacacgattc tttagaattg
      601 gtttacggcg ggatatctcc tgcaaggcaa cttcacctcc ttgttcgtac aactctttaa
      661 aacgataaaa actgtcacgt ctggagcttc tcgcattcgt tgcgccctgt catcagcgta
      721 gcggacaaat ttatgacccc tgctttcaag ctctcggtct aactcgttca gtaggatatt
      781 tcccaaaagt ggacttaatg gtcctccctg cggcacgccc tcgtgagatc tcccgtggta
      841 agataaataa ccttcatccc atgtccccgc cgcatttaca ctccgttgtc cgcataatct
      901 ttggactttg acttgttagg cagtctcctc cagattccat ctcgcgataa acacccttgt
      961 ccttagcnnn nnnnnnnaat ggttggcgat tatgaacccc catagtggac ttgcaccacc
     1021 aagttattta tcatgcacgg cacacctaaa ttgagggaat attaaattcc cctcattttt
     1081 ttctcccact tccaagcaga tctcaaaaca tcatcaatag gtgtcactgc tttccatcct
     1141 agcatattat ttgctttcga aggatccgcc cacacctttt ctatatcccc cgcccttctc
     1201 tcggtaatct tataaggaat ctttatacca gtggcctttt cgaacaaaga aaccatttga
     1261 agtacagata ttccctgtcc tgtgccaagg ttataatact ccatatcccc ttccatttca
     1321 cttctaccac taataagacg atcaagtgcc aatatatgag cctttgccaa atcacacaca
     1381 taaatataat ctcttatgca tgaaccgtca ggagtgttgt aatcatctcc aaacacagaa
     1441 agttcttttc tcacccccgc agcagtctga gtaataaaag gcagtaaatt ttgaggaaca
     1501 cctcgtggta actctccaat taaagaagaa ggatgagctc caatgggatt gaaatatctc
     1561 agagaaacaa ctttcaactt cccttttaaa gagagagcac aatctctaag tatctcctca
     1621 catatttgct tggtattacc ataaggactg gttgccggtt gcaaaggagc attttcgctt
     1681 acgggaagat gatcaggctg accatagact gtacaagaag aagagaatac tatagaagaa
     1741 tcctctctcc ctcgcatcaa atcaatcaga ttgattaaag aattgatatt atttttgtag
     1801 tattcaacag gtttttccac actctcacca actgccttaa acgcagcaaa atgaattgca
     1861 gcccttatgt caggaaaagt tttgaataca ttggcaaaag agtgatagtc agaacaatca
     1921 gcttcaaaaa aaattggagg ttttcctgtt atttgctcaa tcccaagcaa cacatccgga
     1981 gaagaatttg agaaattatc cactattaca gcttcatacc ctgattcata gagttcaaca
     2041 acagtgtgag cccctatata tccggttcct ccagtaacaa gtatcttctg tttgtgcatg
     2101 agattaaaaa ttattggtta atatccaaaa aaatctttta tagcattaca cacttccatc
     2161 tgaatatctt cagtaagctc agtatgcatt ggaagagaga gaacctcatt ggccactctg
     2221 ccagaatttt ccaaagaaac ataaatcttg gaaatgcggt taaatgcttt ttgacgattg
     2281 agaggaagcg gatagtaaat tgtagaagct ataccttttt catttaagaa cttcttaagt
     2341 tcattcctcc cttccttaac tctcaaagta aactgatgat aaacatgagt tgagaaatct
     2401 gattctacag gaagaaccaa ctccctaaca tccgaaagaa gctctttata tctttgagca
     2461 gcttggtacc tcgaagctga atattcattt aggtggtcga gcttaacatt cagaatagct
     2521 gcctgaatgg tgtcaagtct tgaattacag ccaattatgt cgtgataata ctttaccttt
     2581 tgaccatgat ctgtcaccat tcttatcctc tcagcaagag acgaatcttg agtaaaaatt
     2641 gctcctccgt caccataaca gccaagattc tttgtgggga aaaaagatgt acagccaata
     2701 tgcccaatag taccggcaaa tttttcagag ttgtcttcga atctgtattt tgctccaaga
     2761 gcttgagcat tgtcctcaat cacataaaga ttgtattttt gagcaatctc caatatcctg
     2821 cccatgtcac ttgtctggcc aaaaaggtgt acaggcacta tagctcttgt ttttggtgta
     2881 acagaaggtt cgatgtcctc aaccttaata ttaaaagtat ctgaataaac atctacaagt
     2941 acaggaacca aacccaacaa agcaaccacc tcggcagttg cagcatatgt aaaagcagga
     3001 acaatcactt catcaccagg tttaagttcc agtgccatca ttgcaatttg caatgcatca
     3061 gtcccattcg cacaagcaat tacgtgtttg gaaagcaaat attcagaaag attcttttca
     3121 aaattaaaca cctcttttcc attaataaat atggcactgt caataacttt ttgaatagac
     3181 tcatcaacat ctttttttat ttttttgtat tgacccttaa ggtctaacat gggaatagtc
     3241 atatgtattt aatatttatt aaccacaaat ttaaaaatat tannnnnnnn nntaattaac
     3301 tgctatcttt gtaatgttat gttaacaaat gacaagatta tcggaattgc tgcagatcat
     3361 gcaggatttg aattgaaaga gattctaaaa aaggaaatcg aatcaatggg ttttacctgt
     3421 aaggatttcg gcacatattc aactgaaagc atagattatc ccgatgttgc acatcctttg
     3481 gccgaagctg tatcaaaagg cgaaattaca cgtgctgttg ctatttgtgg gacaggcaat
     3541 ggaataagta tggttttgaa caagcacgca ggtatcagag ctgctctctg ttggaatgaa
     3601 gagattgctt cccttgccag acaacataat aatgccaata tctgttctct tccggcacgt
     3661 tttatatcct cttcactcgc catttctata gtaaaaacat tccttactac tgaattcgaa
     3721 ggaggaagac acaaacggcg aattgacaaa atagacatta aataatgcca ttttatattc
     3781 ttgatagaga gcatgttaat gcaaaactct ctactgattt taataattat ccttccggca
     3841 tagttattcc tttggacaaa ccttataaat ggacatctgc agatgctgta aggaaaatca
     3901 aatttatgct gcaaaatttt ttcaaaatca ggaacatcaa ggtcggacat gcaggcacac
     3961 ttgatcccct tgctacagga atcctcctta tctgtgtggg taatgccact aaagaagctg
     4021 aaaaactaca gcaagaagaa aaggagtacc ttgcagagat aacatttgga tgcacaacac
     4081 cctcattcga taaggaaaaa gaaatagatg catattttcc ttatgaacat attgacaaac
     4141 cacttataga aacaagtctg acaaaattta ttggggagca ggatcagata cctccacttt
     4201 tttcggcaaa acttgtagat ggtaaaagag catacgaaat tgcaagatca gggaattcta
     4261 tggaacttaa accttcaaaa attacaatct ccgaattgaa tattgttact tacgaaaagc
     4321 ctcttcttgt tttgcaaata agatgcagca aaggaacata tattcgatct cttgcacgcg
     4381 atcttggtta ttccttagac agcggtgcgt atttaagtgg attggtgaga acttccactg
     4441 gaatcttcaa cttgaaaaat agtgtaaaaa tcgaagaaat tgaatctctt ttgaaacaaa
     4501 tttaaaaata ttacgtataa tttttggttt atataatttt tttaattttt tcaaatgaaa
     4561 ctatctcagt ttaactatcc tctaaatcat aatcaaattg ccaagtatcc agcaaaatac
     4621 agagatgaat caagattgat gatccttcac aaatctacag gtgagattga acatcgtatt
     4681 tttaaggata tattggatta cagtgaagaa ggtgatattt atatttttaa caataccaaa
     4741 gttttcccgg caagattaaa agggaacaag gaaaagacag gagctgaaat agaagttttc
     4801 cttcttagag agttgaaccg cgagcaaaaa ttgtgggatg ttctagttga ccctgcaaga
     4861 aaaatcagaa ttggcaataa actttacttt ggcgaaaatg acaatctggt cgcagaggtt
     4921 attgacaata caacttcacg aggacgaact ttgagattcc tctttgatgg ccctcacgaa
     4981 gagttcaagg aggcactttt caaattaggt gaaatgccca tcccaaaatg gattaggact
     5041 aaagttgaga gcatcgatta tgaaagatat cagactattt ttgcccagaa agagggtgct
     5101 gtagcagctc ctgcagcagg acttcacttc agccgcgaac tgctcaagag gatggaaata
     5161 aaaggtgtaa attcaacatt cataacactg cacatgggac tcggtaactt tcgtacagtt
     5221 gatgttgaag atctttcaaa acacaaaatg gattctgaac aggttgaaat tactgaagaa
     5281 tctgctaatt caataaatga gacaaaagat aaagggaaaa aagtatttgc cattggtgtg
     5341 accgtactaa gagctgtcga aacctccgtt acaactcagg gaagactcaa accatttgag
     5401 ggttggacca acaaatttat ttttcctccc tataatattt cagttcctga cgcacttgtg
     5461 actaatttcc aacttccaca gtcaacaatg ctcatggcag tagctgcatt cggaggatat
     5521 gatcctgtca tgaatgccta tcatgttgca cttaaagaag gttataaatt tggaacctat
     5581 ggtgatgcaa tgttgatact ataattgtat aattaatagc attttctgat tctaaatttt
     5641 atttatccct tttgatgacc ccaaaaacaa cggggtcatt tatttttgca ttatgcaaaa
     5701 tgatgaatta ctttgtactg ttgctattaa caatatattc tgctaccggc cgaaactctc
     5761 aaatactatt ctggaagagt ttggcagttt ttcacctctt ttcagaatga gttattcgga
     5821 actagtagaa cgatttggca agaactatga tttcctctcc aaaataagta atggtaagaa
     5881 tcttgaatac gctgaagaag aaatagcatg ggccaaaaat gagggcataa aaattattcc
     5941 tattagttca gaagattatc cctcaaaatt gaaaggctgt tcagatcctc ctattcttct
     6001 ctatcttctc ggcaatgccc ctttaaacaa caacctgttg atttcgatag ttggtacaag
     6061 aatgcccacg aaatatggaa gtgagtgttg caaaaatata attcaaacca tgaaagagat
     6121 aggcttaaag cctgtgatag tcagtggact tgcatatggg atagatgctg tcgcccacac
     6181 agccgcatta aatgcaagtt taagtacaat cgctattttg ccggggggta tggaaaaaat
     6241 ttaccctgct tcccacaaaa accttgcaga aaaaataatc aataatggtt ctcttattac
     6301 ggaatttcca agaaacagcc agtttcacaa ggttaacttt gtccaaagaa acagaataat
     6361 tgcagggctt tccgaagcga ccattattat tgaatccaga gaacggggag gtgctctaat
     6421 taccgcaaaa ttagcacatt cctattcaag agaggtcttt gctttacctg gaagaattgg
     6481 agacatatgc tctcaaggct gtaacactct tatttcaaaa aatttagcct caatttttac
     6541 ttcgacagaa gatttcgtaa aatctatggg ttgggaatta aagagaagcc atcagccagt
     6601 tcaaaaaaaa ctattttatt atgacgaccc taacaaagaa aaaattatat tagctttgaa
     6661 caatgaatcc gggttgacaa aagatgaaat tatggcaaaa tgccacataa atctgcctct
     6721 tctttcatca ttactgctcg acatggaact cgaaggttct gtcttagcac tgcccggaga
     6781 gagatacagt atcataacac atagatgaaa aagtatatcg atactcactc acatctctat
     6841 gatgccgatt ttgtaacaga tattgatgaa gtgatttcaa gagccgaaga agagggcgtc
     6901 ttttcctgta ttttgcccgg gatagacaaa aagtcacatt ctgatttaat tgcactgtca
     6961 aacaaaaaaa gttcttttgc atatccagcc actggattgc acccaacatc tgtaaataat
     7021 gaatgggaag atgaacttgc gtttgtcttc gataatgcta aaaaaggtat ctacacagcc
     7081 atcggtgaga ttggaatgga tggatactgg tcaaaagagt ttcttgaaca acaatttatt
     7141 gcatttcaaa aacaacttga acttgcagcc atgctgtcac tacctgtcat aatccatgtc
     7201 agagaagcaa ctgaaaagac attttcaatc cttgacaaaa caaaacattt agggttgaaa
     7261 ggagtgtttc atgccttttc gggcagttac gagacattca aaagaacaca aaaatatgga
     7321 gattttatgg ttggcattgg tggtacagtc acatataaaa actccagcct tccttctact
     7381 ctcttaaaaa ttcctcttga ccgcatagta cttgagacag atgcaccttg gctgactcct
     7441 gtgccgttca gaggcaaaag aaatgaaccg tcttttattc ctttcattgc cgacaaaata
     7501 gctgaatcca aaagatgcag tatagatgag gtagcagaga ttacgactgc taatgctata
     7561 agattattta aattacaaaa atgaattcat caatccttat tatttacaca ggtgggacta
     7621 ttggcatgaa gcaagatcct gatactcttg ctcttgtccc atttaaattt gatcagattg
     7681 aagctgaagt ccctgaatta agaaaattcg gctacaaaat agattcctac tcattcgatc
     7741 ctccaattga ttcatctgaa attaatacct ccttctgggt cgaacttgct gaaataatct
     7801 atgaaaatta tgaaaaatat gacggttttg tgatacttca cgggacagat actatgtctt
     7861 attctgcctc tgcactgagt tttatgctcg aaaatcttga gaaacccgtg atattcacag
     7921 gaagtcagct tcccataggg atgcttcgta ctgacggaaa agagaatctt atatcttcaa
     7981 tagaaattgc agcagctcgt gacgacaatg gcagagcaat agtcccagaa gtttgcgtat
     8041 actttgaaag cagactgtat agagggaacc ggacaacaaa ggataatgct gaaaatttcc
     8101 gggctttcag atcagccaat tacccatatc ttgcagaagt aggcatacat attaaatata
     8161 atagaaactt catatcatat cctgaaaagt ggggacagca atttaaaatt aataccagtc
     8221 ttgacacaaa tgttgcaatt ctcaaaatct ttcccggtat ctctcaatat gtggtagaaa
     8281 gctttctaag aataaatgga ttgagagcca taatacttga aacctatggt tcaggaaatg
     8341 ctcccacatt tgactggttg atatctttgc ttaaaaattc catcaaccaa ggtcttatta
     8401 ttttgaatgt aactcagtgc cccgcaggga gtgtaaatat ggaagcatat tctacaggaa
     8461 ttctgttaaa aaaaatagga gttcaaagtg gttacgacag tacaacagaa gcagcattga
     8521 caaaattatt ttttattcta ggtcataact ttgacaatca aaatattgta aatttattat
     8581 caaaaaattt aaggggtgaa atttcaatag attagtaatt ttataaaatt ctttataaat
     8641 tgcatcgaat ttaacttttg gttaaaattg ataactattt aatatacatt gtttaatact
     8701 ttaattattc aaaaatttat gaaaaaattt ttcatgtttt tggcagttgt gggattaata
     8761 gccatttctg ctcagtatgt ttctgctcag acacctgatt ctgctgcaac aacaacaagt
     8821 gttgccgccg cttctactgc cgctccttct tctgaccaga cagttcaaaa agagattccg
     8881 attcaccaac agcttaagac aaaatttatc gagggtggcc cgggatttat ggctgcaatt
     8941 ctcctgtgtc ttatcatagg tctggcaatt gccattgaaa gaatcattta cttaaacctc
     9001 gcatcaacaa atattgaaaa actattgaaa caaattgaag atgcactcgc tagtggtgga
     9061 gttgaagcag caaaagaagt ttgccggaat actaaaggtc ctgttgcaag cattttctat
     9121 gaagggctca gccgtgcaga cgagggaatc gagatggttg aaaaatccat ttcttcttca
     9181 ggttcaattc aaatgtcaca gctcgaaagt ggccttaact ggataaacct ctttatttcc
     9241 atagcaccta tgttaggatt cctcggtaca gttatcggac tggtcatggc atttgatgat
     9301 attcaaaaag ccggtgatat ctctcctaac atcgtagcag gaggtatgaa agtggcgttg
     9361 atcacaacta ttggaggtct tatagttgcc attattcttc agcttttcta ctcttatatc
     9421 gtttcaaaga ttgactctat agttctttca atggaagact catccgtctc attgatagac
     9481 atacttgtaa aaagtaaaaa atagcagatt atgcaaaaag ctttaaaaat attttca
//
LOCUS       DAOTQL010000060         9280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6544_length_9280_cov_4.631545, whole genome shotgun sequence.
ACCESSION   DAOTQL010000060 DAOTQL010000000
VERSION     DAOTQL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6544_length_9280_cov_4.631545"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="rplJ"
                     /locus_tag="P5293_04785"
     CDS             <1..379
                     /gene="rplJ"
                     /locus_tag="P5293_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRR49250.1"
                     /translation="LVVVKNTLLRKTLEQKGINEAEKLFPVLEGPSAVMFTEEINAPA
                     KLIKDFGSQYGKPVLKAALVQDCAYVGANQLDALIAIKSREELIGDIIGLLQSPVKNV
                     ISALQSNAGGKVAGIVKTLSEKE"
     gene            414..791
                     /gene="rplL"
                     /locus_tag="P5293_04790"
     CDS             414..791
                     /gene="rplL"
                     /locus_tag="P5293_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRR49251.1"
                     /translation="MADIKKFAEELVNLSVKEVQELAKVLKEEYGIEPAAAVAVAAPA
                     AGGAAPAGEAEQTQFDVILTSAGQTKLQLIKVVKEITGLGLKEAKDLVDAAPKAIKEK
                     ISKAEAEQVKAQIEEAGGEVEIK"
     gene            898..4716
                     /gene="rpoB"
                     /locus_tag="P5293_04795"
     CDS             898..4716
                     /gene="rpoB"
                     /locus_tag="P5293_04795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRR49252.1"
                     /translation="MSQKTNNQRISFATSKVLLDYPDFLEVQLKSFKDFFQLETTPEN
                     RKNEGLFKVFQEIFPITDTRNNFVLEFIDYFIDPPRYSIEECLDRGLTYSVPLKAKLK
                     LYCTDPEHEDFDTVIQDVYLGTIPYMTPRGTFVINGSERIVVSQLHRSPGVFFGQSLH
                     ANGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGYESDKDILD
                     IFGLANEVKVTKANLKKCIGSKLAARVLKTWNEDFVDEDTGEVVYIERNEIVLDRETV
                     IEEEHIDTILESGVSTILLHKEDANINDYAIIFNTLQKDQCNSEKEAVFYTYRQLRNS
                     EPPDEATARDVIDKLFFSDKRYDLGEVGRYRLNHKLGLSIPDEIRVLTKADIIAIIKY
                     LIQLINSKAAVDDIDHLSNRRIRTVGEQLANQFSVGLSRMARTIRERMNVRDNEVFTP
                     IDLINAKTLSSVINSFFGTSQLSQFMDQTNPLAEMTHKRRLSALGPGGLSRDRAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINDLGFIETPYRKVNNGIVDMTQEG
                     CIYMTAEDEEEKMVAQANVNIDENGDILDERIKCRFEADYPVVGKEEVDLIDVAPNQI
                     ASIAASLIPFLEHDDANRALMGSNMMRQAVPLISPEAPIVGTGLEGPVIKDSRTQIVA
                     RGKGEVVYVDAREIHIKYERTENDKFVSFEPEITVYKLPLYRKTNQNTSITLKPIVRK
                     GDKVVKGQIMTEGYATQNGELALGRNLKVAFMPWKGYNFEDAIVLSERLIREDVFTSI
                     HIDEYIMEVRDTKRGMEELTSDIPNVSEDATKNLDENGIIRIGANVEPGDILIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKASPSLSGTIIDKRLFSRVTKESNKKGKS
                     TSKNQMQQAEENFERKTAALRQNFLERLYTLLEGKQSQGINDLYGAEIIGKGVIFKID
                     LLDKIDYENINPVKWTSDKNTNNLIKQLINNYLIAYKEYDAELKRIKYNITIGDELPT
                     GIMQLAKVYVAKKRKIRVGDKMAGRHGNKGIVAKVVRDEDMPFLENGKPVDIVLNPLG
                     VPSRMNLGQIYETVLGWAGVELNEKFSTPIFDGATLEDICNYTDKAGVPRFGKTYLRD
                     GGTGELFDQPATVGVIYMMKLGHLVDDKMHARSIGPYSLITQQPLGGKAQNGGQRFGE
                     MEVWALEAFGAANTLQEILTIKSDDVTGRSRAYEAIVKGEPMPTPGIPESLNVLLHEL
                     RGLGLSIRLD"
     gene            4751..9010
                     /gene="rpoC"
                     /locus_tag="P5293_04800"
     CDS             4751..9010
                     /gene="rpoC"
                     /locus_tag="P5293_04800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRR49253.1"
                     /translation="MPAKKEIKVKSNFNQITIGLASPEEILERSSGEVLKPETVNYRT
                     YKPERDGLFCERIFGPYKDYECHCGKYKRIRYRGIICDRCGVEVTEKKVRRERMGHIS
                     LTVPVAHIWYFRSTPNKIGYLLGIPSKKLEAIIYYERYVVMQPGIKEKEGVKVNDLLT
                     EDEYLTIIDSLPKDNQLLDDSDPNKFIAGMGAEIIYKMLTRINLDDLSYELRHKTESE
                     TSQQRKAEALKRLGVVEAFRESKEINKPEWMILKVIPVIPPDLRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTEANR
                     ALKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVDRKDPVIWDILENVMKGHPVMLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLACTAFNADFDGDQMAVHLPLGNAAILEAQLLMLGSHNILNPANG
                     APITVPSQDMVLGLYYITKKRSGSKGEGMKFYGPEEVIICYNEGKLDLHAEIEVRLPK
                     NMCDLSQGYEMVKTTTGRVIFNQVVPKEVGYINELLTKKFLRDIISNVLKVSGVARTS
                     QFLDDIKEMGFSMAFKGGLSFNLADVLVPQEKEALIEDGYKQVENIMASYNMGLITNN
                     ERYNQIIDIWTRTNSKLTNIVEKQISLDQQGFNPVYMMLDSGARGSKDQIRQLTGMRG
                     LMAKPQKSGATSSEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLT
                     RRLVDVSHDVIITEEDCGTLRGLVATAIKNKDEVVESLYERIMGRSTVHDIYNPLTGE
                     KIIEAGEMITEEIAALIQSLPIESVEIRSVLTCESRKGVCAKCYGRNLATGRMVEKGE
                     VVGVIAAQSIGEPGTQLTLRTFHVGGTASSVASENEIIARYEGRLEFEELRTITSVND
                     DGSTREIVIGRTAEMKIVDIHTGITLSTYNIPYGATLFFKEGDIVKKGDKICEWDAYN
                     AVTIAETGGKVVFDNMIEGVTYREEAADEYSLHKEKVIIESRDKSKNPTIRIMDGQVE
                     LRQYNLPVGAHIMVENGKRVKAGDILVKIPRAVGKTGDITGGLPRVTELFEARNPSNP
                     AIVTEINGEVIMGKIKRGNREIIIRSKTGEEKRYLVPLSKQILVQENDYVRAGMALSD
                     GAISPTDILAIQGPTKVQEYIVNEVQDVYRSQGVKINDKHFEIIVRQMMRKLEIVDPG
                     DTRFLPEQLVDKWEFIHENDEIYDKKVVLDAGDSTTLKPGQIVTVRRLREENSILKRK
                     DLKLVEARDAMPATSNQILQGITRAALRTNSFMSAASFQETTKVLSEAALHGKTDTLE
                     GLKENVICGHLIPAGTGQREFEKIIVTPMDDYQKLVKSKKE"
     gene            complement(9070..>9280)
                     /locus_tag="P5293_04805"
     CDS             complement(9070..>9280)
                     /locus_tag="P5293_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49254.1"
                     /translation="DIFMTYSDAPYLSGCISQNNLERISGTPAIITETIGKGKIIYMA
                     DDLNFRSYWFGGSKIFMNAIFFGYL"
BASE COUNT         2974 a         1733 c         2024 g         2549 t
ORIGIN      
        1 acttgttgtc gttaaaaaca ccttgctgcg taaaactttg gaacaaaaag gcattaacga
       61 ggctgaaaag ctttttcctg tccttgaagg cccttctgca gtgatgttta cagaagagat
      121 aaatgctccc gccaaactta tcaaggattt tggcagccaa tacggtaaac ctgtgcttaa
      181 agcagccctg gttcaagatt gtgcatatgt tggtgcaaac cagcttgacg cacttattgc
      241 aattaagtca cgcgaagagc ttatcggtga tattatcggt ctgcttcaat cacctgtcaa
      301 aaatgttatt tctgcacttc aatcaaatgc aggaggtaaa gttgcaggta ttgtgaagac
      361 cttatcagaa aaagagtaat aaaaattaaa taaacttaat aattaaatca attatggcag
      421 atatcaaaaa atttgcagaa gaattagtta acctgtctgt aaaagaggtg caagaacttg
      481 caaaagtcct taaagaagaa tatggtattg aacctgctgc agctgttgct gttgctgctc
      541 ctgcagccgg tggtgctgct cctgccggag aggctgagca gactcagttt gacgttatcc
      601 ttacttcagc cggtcagact aaacttcagc tgattaaagt cgttaaggag atcacaggtc
      661 ttggacttaa agaggccaag gatcttgttg atgctgctcc taaagcaata aaagaaaaaa
      721 tatcaaaagc cgaagctgaa caggttaagg cacaaattga agaggccggc ggagaagttg
      781 agattaaata actccaaaat cccccgttaa tcgggtacaa taacaggttt agagccatat
      841 accggctcta agcctttttg tcgttatttc actatatttt caaccaatcc acatacaatg
      901 tcgcaaaaaa caaataatca gcggatttca ttcgcgactt caaaagtcct actcgactat
      961 ccagatttcc tcgaggttca gttgaagtct ttcaaagact tcttccaact tgaaactacg
     1021 cctgaaaatc gtaagaatga aggcttattc aaggttttcc aggaaatttt tcctatcact
     1081 gatactcgta ataattttgt acttgagttt attgactatt ttattgaccc tccacgttat
     1141 tcaatagagg agtgtctcga cagaggtctg acatacagcg tacctcttaa agctaaactc
     1201 aagctttact gtaccgatcc tgagcatgag gattttgata cggttattca ggatgtctat
     1261 ctgggaacta ttccttacat gaccccaagg ggtacttttg tcatcaacgg ttctgaaaga
     1321 attgtcgtat ctcagttgca cagatcccct ggcgtatttt ttggccaaag ccttcacgca
     1381 aacggaacaa aactatattc cgcaaggatt attcctttca agggttcatg gatcgagttt
     1441 gctactgata ttaataatgt catgtatgcc tacatcgaca gaaagaaaaa attacctgta
     1501 actactttgc taagagcaat aggatatgag agtgataaag atatactcga catattcggt
     1561 ctcgcaaatg aggttaaggt tacaaaagca aatttaaaga agtgtatcgg cagcaaactg
     1621 gctgccagag ttttaaaaac atggaacgaa gatttcgttg atgaagatac cggtgaagtg
     1681 gtttatattg aaagaaacga gattgttctt gaccgtgaaa cagttattga agaggagcat
     1741 attgacacta tccttgaatc aggagtaagt acaatacttc tccacaagga agatgcaaac
     1801 attaatgact atgccatcat tttcaatacc ctccagaaag accagtgcaa ttcggaaaaa
     1861 gaggctgttt tctataccta caggcaactt cgtaattctg aaccgcctga tgaagctact
     1921 gcaagagatg ttattgacaa acttttcttt tcagataaga gatacgattt gggagaagtg
     1981 ggaagataca gactaaatca taaactcggt ctttctatcc ctgacgagat aagggttttg
     2041 actaaagccg acattattgc aattattaaa taccttatcc aacttataaa ttcaaaggca
     2101 gcagttgacg atattgatca tctaagcaac cgccgtatca gaactgtcgg tgaacagctt
     2161 gcaaatcaat ttagcgtagg cctttcacgt atggcacgta caataaggga aagaatgaat
     2221 gtcagagaca atgaggtgtt cactcctatt gatcttatca atgccaaaac tctatcttcg
     2281 gttattaatt cattttttgg taccagccag ttatctcagt ttatggatca aacaaatcca
     2341 ctggctgaaa tgactcataa aagaagactc tctgctcttg gtccaggagg tctttcaaga
     2401 gatcgtgcag gatttgaagt tagagacgtg cactatactc attatggtcg cctctgccct
     2461 attgaaaccc ctgaagggcc taatattggt ctgatttctt ctctttgcgt atatgccaaa
     2521 atcaacgatc ttggttttat cgaaactcct tacagaaagg tgaataacgg aattgtagat
     2581 atgactcaag agggctgtat ttatatgact gctgaagatg aggaggagaa gatggttgct
     2641 caggcaaatg tcaacataga cgaaaatggc gatatactcg atgagagaat taaatgtcgt
     2701 tttgaagcag attatcctgt tgtcggaaaa gaagaagtag atcttattga tgtggctccc
     2761 aatcaaatag cctcaatagc cgcttcattg attcctttcc ttgaacacga cgacgcgaat
     2821 cgtgccttga tgggttcaaa catgatgcgt caggctgttc ctcttatctc acctgaagca
     2881 ccaatagtcg gtacaggtct ggaaggtcct gtcattaagg attcacgtac tcagattgtt
     2941 gccagaggta agggagaagt tgtttacgtt gacgctcgtg agatacatat aaaatacgag
     3001 cgcactgaaa acgacaaatt tgtaagtttt gaacctgaaa ttacggttta taagcttccg
     3061 ctttacagaa aaaccaacca gaatacttca attactctta aaccaattgt aaggaaagga
     3121 gataaggtgg ttaaaggtca aattatgaca gaaggctatg ctactcaaaa tggagaactt
     3181 gcacttggca gaaatctcaa agttgcattt atgccatgga aaggatataa ttttgaagat
     3241 gctattgtgc tttctgaaag gttgataaga gaagatgtct ttacttccat tcatatagat
     3301 gaatatataa tggaggtacg cgatactaag aggggaatgg aagagttgac ctctgatatt
     3361 ccaaatgtaa gtgaagatgc gaccaaaaat cttgacgaaa atggtataat aagaatcggc
     3421 gctaatgtgg aacccggaga tatccttata ggtaaaataa ctcctaaggg tgagagtgac
     3481 ccttctccag aagagaaact gctaagagca atttttggag acaaagccgg ggatgttaaa
     3541 gatgcatctt taaaagcttc tccttctctg agtggtacta tcatcgacaa gcgtctattc
     3601 tcaagagtta caaaggagag taacaagaag ggcaaatcta cttctaaaaa tcagatgcag
     3661 caggctgaag aaaattttga aagaaaaact gcagccctgc gtcaaaattt cctggagaga
     3721 ctctatactc ttctcgaggg caagcagtcg caaggtatca acgacctcta cggagctgaa
     3781 attatcggta aaggtgtaat attcaaaata gaccttctcg acaagataga ctatgaaaat
     3841 atcaatcctg tcaagtggac ttcagacaaa aacaccaata atctcattaa acagttaatc
     3901 aataactacc ttattgcata taaggagtat gatgccgaac ttaaaaggat taagtacaac
     3961 attactatcg gtgatgagct tcctacaggt attatgcagc ttgcaaaggt ctatgtcgcc
     4021 aaaaagagaa aaatacgtgt tggtgacaag atggccggaa gacacggaaa caagggtatt
     4081 gttgccaaag ttgttcgaga tgaggatatg cctttcctcg aaaatgggaa acctgttgat
     4141 attgtcttaa atcctcttgg agtgccttct cgtatgaatc tcggacaaat ctatgaaact
     4201 gtgcttggat gggccggagt agaacttaac gagaagttca gcacacctat ttttgatggt
     4261 gcgactcttg aagatatatg caattacaca gataaagcag gagttccaag atttggaaaa
     4321 acttatctaa gagatggtgg tacaggtgaa ttgtttgacc agcctgcgac tgtaggtgtt
     4381 atctatatga tgaaacttgg tcacttggtt gacgataaga tgcacgcccg ttccataggg
     4441 ccttattcgc tgattaccca acagccgctc ggtggtaaag ctcaaaacgg aggtcagaga
     4501 tttggtgaaa tggaagtttg ggcattggag gcttttggtg ctgccaatac ccttcaagaa
     4561 attctgacca ttaaatctga tgatgtaacg ggaagatcaa gagcctatga ggctattgtg
     4621 aaaggagaac caatgcctac cccggggatt cctgaatcac tcaatgtgct ccttcatgaa
     4681 cttcgtggct tgggattaag catcagactg gattaattaa gtagtgtttg acaatttgaa
     4741 tcagaaaaat atgcctgcaa agaaagagat aaaggttaag agtaatttta atcaaattac
     4801 aataggcttg gcttcgcctg aagagatcct tgagagatca tcaggtgagg ttctcaagcc
     4861 tgagactgtt aactaccgta catacaagcc cgaaagagac ggcctattct gtgaaaggat
     4921 ttttggacct tataaggatt acgagtgcca ttgcggtaaa tataagagaa tccgctaccg
     4981 tggaattata tgcgatagat gtggtgttga ggttactgaa aagaaggtga gacgtgaaag
     5041 aatgggacat ataagtctca ccgtacctgt tgcacatata tggtacttcc gttcaactcc
     5101 caacaaaata ggatatctgc tcggtattcc ttccaaaaag ctcgaagcaa ttatatacta
     5161 cgaaagatat gttgtgatgc aacccggcat caaggaaaaa gaaggtgtta aggttaatga
     5221 ccttcttacc gaagacgaat atcttactat tattgactct ctgcctaagg acaaccagct
     5281 tctggacgat agtgatccta ataagtttat tgccggaatg ggtgcggaaa tcatatataa
     5341 aatgcttacc cgtatcaatc tcgatgatct ttcatacgag ctgcgccata aaactgaatc
     5401 tgagacttct cagcaaagaa aagctgaggc tcttaaaagg ctcggtgtgg tagaagcctt
     5461 ccgtgaatca aaagagataa ataaacctga gtggatgatc ctcaaggtta tcccggttat
     5521 cccgccagat ctaagacctt tggttcctct cgatggagga cgctttgcta caagtgacct
     5581 taacgatctt tacaggagag ttatcattcg taacaacaga cttaaaaggc ttatagagat
     5641 caaagcacct gaagtgatac ttcgtaacga gaagagaatg ctgcaagaag ctgttgactc
     5701 tcttttcgac aattcaagaa aatccaatgc agttaagaca gaagctaacc gcgctcttaa
     5761 atcgctttca gacagcctta agggtaaaca gggacgtttc cgtcaaaatc tgcttggaaa
     5821 aagggttgac tattctgccc gttcagtcat tgttgttggt cctgaactta aaatgcatga
     5881 atgtggtcta cctaaagata tggctgcaga actatacaaa ccttttatta tcagaaaact
     5941 gattgaaaga ggtattgtta agactgtcaa gtcagccaaa aagattgttg acagaaagga
     6001 tccggttatt tgggatatcc tcgagaatgt tatgaaaggg catcctgtca tgttgaaccg
     6061 tgctcctacc ttacacagac tcggtattca ggcgttccaa ccaaagctca tcgagggcaa
     6121 agccatacag cttcatcctc tggcctgtac tgcatttaat gcagactttg acggtgacca
     6181 gatggctgtt cacctgccac ttggtaatgc tgcaatactc gaagcacagc tactgatgct
     6241 tggatctcat aatatcctta atcctgcaaa cggggctcct atcacagttc catctcagga
     6301 catggtgctt ggattgtact atataaccaa gaaacgctct ggttcaaaag gagaagggat
     6361 gaagttctat ggtcccgaag aggtcattat ttgttataac gaaggcaaac ttgaccttca
     6421 tgcagagata gaggtgagac ttcctaaaaa catgtgtgat ttgtctcaag gttatgaaat
     6481 ggttaagaca acaaccggac gtgtgatttt taatcaggtt gttcctaaag aagtaggtta
     6541 tattaacgaa ctgcttacca aaaaatttct tcgcgatata atcagcaatg tgctgaaagt
     6601 ttctggagtc gccagaacat cacagtttct cgatgatatc aaagagatgg gatttagtat
     6661 ggctttcaag ggaggacttt cattcaatct tgcagacgtc ttagtcccac aggaaaaaga
     6721 ggctcttata gaagacggat acaaacaggt tgaaaatata atggcttctt acaatatggg
     6781 ccttatcacc aataatgaaa gatataacca gataattgat atttggacaa gaaccaattc
     6841 aaaacttact aatatagtag aaaaacaaat ctctcttgac cagcagggat ttaatcctgt
     6901 atatatgatg ctggactcag gagctcgtgg ttcaaaagat cagattcgtc agttgaccgg
     6961 tatgcgtggc ttgatggcaa aacctcaaaa atcaggtgct acaagctctg aaattatcga
     7021 aaatcctatt ctctccaact ttaaggaggg actttctgtt cttgaatact tcatctccac
     7081 tcacggtgcc aggaaaggtc ttgcagatac tgctctcaaa acagcagatg cagggtatct
     7141 taccaggaga ctggttgatg tctctcacga cgtaattatt actgaagagg attgtggtac
     7201 tctgcgtggt cttgtagcta cagccataaa gaacaaggat gaggtggttg aatctctata
     7261 tgagagaatt atgggtcgtt ctacagtgca tgatatttat aatcctctta ccggtgaaaa
     7321 aataattgaa gccggagaga tgattacaga agagattgcg gcattaattc aatctcttcc
     7381 tatcgaatcc gttgaaattc gttcagtact tacttgcgaa tcccgtaaag gagtatgtgc
     7441 taaatgttat ggacggaacc tcgccaccgg acgtatggta gaaaaaggag aagtcgtagg
     7501 agttattgct gctcaatcta ttggtgaacc tggtactcag ctgacactcc gcacatttca
     7561 cgttggtggt actgcttcaa gcgttgcttc agaaaatgaa attatagcac gttatgaagg
     7621 tcgtcttgaa tttgaggaac ttagaactat tacttcagtt aatgacgatg gttcaacaag
     7681 agagattgtg ataggacgaa ctgccgaaat gaaaattgtt gacatacata caggcattac
     7741 actttcaaca tacaatatcc cttacggggc aactcttttc ttcaaagagg gagacattgt
     7801 aaaaaaagga gacaaaatat gtgagtggga tgcctataat gctgttacaa ttgccgaaac
     7861 aggtggcaaa gttgtttttg ataatatgat agaaggtgta acctatcgtg aagaagctgc
     7921 agatgagtac tctcttcata aggaaaaggt tattatcgaa tcaagggata agtcaaagaa
     7981 tccaaccata cgcattatgg acggacaggt tgaacttagg cagtacaatc ttcctgttgg
     8041 ggcacacatt atggtggaaa acggtaaaag ggtcaaggct ggtgacatac ttgtcaagat
     8101 tccacgtgct gttggtaaaa ctggtgatat cacaggaggt cttcctcgtg ttacagagct
     8161 ttttgaagca cgtaacccat caaaccctgc aattgtaaca gagataaatg gagaggttat
     8221 tatgggcaag attaaaagag gtaaccgtga aattattatc aggtcaaaga caggagaaga
     8281 aaaacgttat ttagttcctc tctccaaaca gattctggta caggagaacg attatgtacg
     8341 tgctgggatg gcactgtcag acggagccat atctccaacc gacatacttg ccattcaggg
     8401 gcctacgaaa gttcaggagt atattgtgaa tgaggttcag gatgtttacc gaagtcaggg
     8461 tgtgaagata aatgataaac attttgaaat cattgttcgc caaatgatgc gcaagcttga
     8521 aattgtggac cctggtgata cacggttcct ccctgaacag cttgtggata aatgggaatt
     8581 tatccatgaa aatgatgaga tttacgacaa aaaagttgtg cttgatgccg gtgattctac
     8641 tacattaaag cctggtcaaa tagttacagt aaggagactt cgcgaagaga attcaattct
     8701 gaagcgtaag gatcttaagc ttgtggaggc aagagatgcc atgcccgcta cttccaatca
     8761 gatacttcaa ggtatcaccc gagctgctct tagaacaaac agctttatgt ctgctgcatc
     8821 attccaggag acaacaaagg tattgagtga agctgctttg cacggcaaga ccgatactct
     8881 cgaagggctt aaggaaaacg ttatctgcgg acatttgatt cctgccggca caggacaaag
     8941 ggaatttgaa aagataattg taactcctat ggatgattat caaaaacttg tcaaaagcaa
     9001 aaaagagtaa ccataattgt aaatcaataa agagggtgtt tgagaattta tctcgacacc
     9061 ctcaattgtt tatagatatc caaaaaatat ggcattcata aatatttttg accctccaaa
     9121 ccaataagaa cgaaaattta gatcgtcagc catatagatt attttgccct tgccaatggt
     9181 ttctgttata atggcaggag taccggatat tctttccaga ttattctgag atatgcatcc
     9241 tgaaagatag ggagcatcag aataggtcat aaaaatgtca
//
LOCUS       DAOTQL010000061         9204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6601_length_9204_cov_4.755602, whole genome shotgun sequence.
ACCESSION   DAOTQL010000061 DAOTQL010000000
VERSION     DAOTQL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6601_length_9204_cov_4.755602"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /gene="pruA"
                     /locus_tag="P5293_04810"
     CDS             <1..1443
                     /gene="pruA"
                     /locus_tag="P5293_04810"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HRR49255.1"
                     /translation="MPHNHSHTLAVYHKAGEKEVKMAIDAAMKAHVLWSEVNWATRAS
                     IMLKIAELLSTKYRATINAATMLGQSKNIYQAEIDSVCETIDFFRFNVAFANEIYKNQ
                     PISTNSQINRMEYRPLEGFVFAVSPFNFTSIACNLNMSPVLMGNTTLWKPATTAILSN
                     YWLMKIFMEAGLPAGVVNFLPGSGALIGNVVLNSKDFGGLHFTGSNATFNSLWQQVGE
                     KLSSYKSYPRLVGETGGKDYIFVHPSADVHEVATAAFCGAFEFQGQKCSAASRMYVPQ
                     SLWGKIKNEMTVLSEEVKMGDVRDSGNFINAVIDEASFDNIVSYIEYVKQSNDAEIIF
                     GGTYNKSVGYFIAPTLVQTTDPHFKSMEEEIFGPFLTVYVYDDDKLEEAAELCNTTSP
                     YGLTGSVFGKDRIEVMKLCSKLTYSAGNFYINDKPTGAVVGKQPFGGSRASGTNDKAG
                     SLFNLIRWISPRAIKETLVPPTDIFYDYMK"
     gene            complement(1452..2429)
                     /locus_tag="P5293_04815"
     CDS             complement(1452..2429)
                     /locus_tag="P5293_04815"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRR49256.1"
                     /translation="MKSKLNLANLPTKIEKLNRFSEDLGINLYIKRDDQTGTEFSGNK
                     IRKLEYVLKEVVDLNYDVVITCGGIQSNHARATVASATKIGLKSVVLLRTDEVPKVEG
                     NYFLDKLLGADVRFCTSEEYKESRAEIMKNIASEYAFKGMRAYIIPEGASFGIGALGY
                     YSAMEEIVLQEKEMGINFNTIVVATGSGGTYAGLYLANKIHGYGKRIIGMAVCDDAVY
                     FKNVVHEISNDALEYLDAEVDISKDEIEINDKYIGIGYALSTKEELDFITMFARKEGV
                     ILDPVYTGKAMRGLFSEVRNLKLKDPGNVLFIHTGGLFGLFPKQDQFDL"
     gene            2501..5554
                     /locus_tag="P5293_04820"
     CDS             2501..5554
                     /locus_tag="P5293_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthan lyase"
                     /protein_id="HRR49257.1"
                     /translation="MKRSLVLLILTVSFILPFKSAAQIQEKSKFKLIADSVNVYLKPL
                     AKVKGIIEIKSVNLSRKRRLDITFNSTLGEYPLREEQIETIYSIVNTLLPPEYRDNKI
                     GIFCNGSKIEELFPSFLNPDQNKSGKAERKVKRDKGKPKTSNLITKGSLPYDISKGLQ
                     NRHIAMWQSHGYYYEQKLLRWEWQRARIFETVEDLYTQSYVLPFLVPMLENAGATVML
                     PRERDTQLNEVIADNDDSDSGYSETNGNSIWVTSSVPGFKNAKEKYIQGENPFMMGTA
                     RLVHFRKKGESAGAKYSPFFPENGEYAVYVAYQSFPQSTERAVYTVHHKGGDTRFYVN
                     QKMGGGTWIYLGTFLFEKGRNWDGCVEIYNVSSHKDEIVSTDAIKFGGGMGNIARSPA
                     SQGSQTNVPSSSHETAKTVVMPNDIIPEGSGYPRFTEGARYWLQWAGFNDTIYSPNAG
                     ANDYNDDYMSRGRWVNVLSGGSSQNPVEKGYNIPIDLSFAFHSDAGTTLNDSIIGTLA
                     IYTRLSNGDSVFPTGTPRITSRYLADMVQTQIVNDVRALYEPIWERRGLWDRSYSESR
                     TPKVPSMLLELLSHQNLADMRYGLDPTFRFTVSRAVYKGILKYLSLSQNREYVVQPLP
                     VKNFSAEISDNNIVKLNWSAVADSLESTAVPTGYVVYRRINNGGFDNGRLVNDTLFSE
                     KIETGKIFSYKISAINDGGESFPSEILSVYKAPDEKGKVLIINCFDRLSAPASFATKD
                     TTRGGFTDFIDHGVPYIKDISYIGSQYEFRRQIPWIDDDSPGFGASYADYEKRLIAGN
                     TFDYPYVHGKALASLGYSFVSCSRDALIAGKVVLNEYPIADFIFGKQLQTKAGRGVKP
                     VKYSVFPKQLQVLIKDYAVQGGKLIVSGANIATDIWDSIEMDQESMDFAQNVLKYKWR
                     TNCASKTGEVRAVQSPYKFDGQYLFHTTPNEYVYCAESPDGLEPVGENSWTVFRYGDN
                     NISAGVAYRGSYSSVVLGFPIETLTTDEQIKNLFRTILKFLKGEN"
     gene            5550..6860
                     /locus_tag="P5293_04825"
     CDS             5550..6860
                     /locus_tag="P5293_04825"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRR49258.1"
                     /translation="MSKNRELEIIELIKREVKPALGCTEPIAVSLAVAKAAEILRERG
                     FEPESVVVEVSANILKNGMGVGIPGTGMVGLHIAAALGITCGKSAYKLEVLQDINEGA
                     IERAKKMISEEKVDIILAKTRKKLHISAKCICNDHSATVVIENQHDSIVYANYDNQII
                     FDNRHNSSANTKFGESQIRDTLDYKLDVATIYQFAKQVDFKDIEFILDSVTLNKALAV
                     EGLKNNYGLKVGKTILDNIHKNIFGEGLLTYSMAMTAAASDARMAGCTLPAMSNSGSG
                     NQGITATMPVISASEKFGSNSEELARALVLSHLIAIHIKGYLGRLSALCGCVIASTGA
                     ACGIVFLNNGTYEQICYAIKNMIGNITGMVCDGAKVGCALKVASGVSSAVQSAILALE
                     GTCISENDGIIEKNIEKTIKNLGKIGSIGMQNTDNMILDIMVCK"
     gene            6960..8969
                     /gene="htpG"
                     /locus_tag="P5293_04830"
     CDS             6960..8969
                     /gene="htpG"
                     /locus_tag="P5293_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRR49259.1"
                     /translation="MQKGTIGVTTENIFPVIKKFLYSDHEIFLRELVSNAADATQKLK
                     ALISSGEFKGDAGDLKIVITSDPEKGIITVSDRGVGMTEDEVNRYINQIAFSSAGEFL
                     DKYKDQINNIIGHFGLGFYSCFMVAKKVEIQTLSWKEGAEPVKWSCEGTPEYQITKGK
                     RKDRGTDIILHLDDESKEFATENKIKELLYKYCRFLPIEIVYGKEEKIINNTNPIWTR
                     KPAELKDEDYIEFYHELYPMLEDPLFYIHLNVDYPFHLTGVLYFPKLKDNLDISRNKI
                     QLYCNQVFVTDSVENIVPDFLTLLHGVIDSPDIPLNVSRSYLQSDSNVKKISSHITKK
                     VADRLEEIFKNDRKNFEDKWDNLKLFIEYGMLSEEKFYERAEKFALFKNCDDKYFSYE
                     EYRKFIESSQTDKNKRLIYLYTTNKEEQYPYIEAARNKGYDVLVFDNQLDAHFINLLE
                     SKFKDSSFVRVDADHIDKLIQKNEISKPDLSEKEVNDISTAFETVLPKGYHYNFSVEN
                     LGENDGALLLTRSEFMRRYREMAALNGGMNFYSSIPESYNVIINYQHPLVKRVIEMMN
                     RELSSKLKDLDEKAAVHDKRLSTIREETSGKKDEEIDTVVKDERKALEEELNKIKDER
                     KELIADFAKKCELIKELADLALLANGTLKGEDLDKFIKRSLSLIK"
BASE COUNT         3112 a         1531 c         1768 g         2793 t
ORIGIN      
        1 atgcctcata atcactcaca tactcttgcg gtatatcata aagcaggaga aaaagaggtt
       61 aagatggcaa tcgatgctgc catgaaggca catgttttat ggagtgaggt aaactgggca
      121 acaagagcgt ctataatgtt aaaaatagca gaattgctgt ctacaaaata cagggctact
      181 attaatgctg ccacaatgct tggtcaaagt aaaaacatat accaggcaga gattgattca
      241 gtttgtgaaa ctattgattt ttttaggttt aacgttgctt ttgcaaatga aatatacaaa
      301 aatcagccga tttcaactaa cagccaaata aacagaatgg aatacagacc tcttgaagga
      361 tttgtattcg ctgtcagccc ttttaacttt acatcaatag cctgcaatct caacatgtct
      421 ccggtactta tgggaaacac aactctgtgg aaaccagcaa caactgctat cctttcaaat
      481 tactggctca tgaaaatctt catggaagca gggttacctg ccggcgttgt aaatttcctg
      541 cctggaagtg gagctcttat tggtaatgtt gttcttaatt caaaagactt tggcggtctc
      601 cattttacag gttcaaatgc tactttcaac tctttatggc aacaagttgg agaaaaactg
      661 tcatcctaca aatcttatcc aagactagtt ggagaaacag gtggcaaaga ttatattttt
      721 gtccaccctt ctgcagatgt tcatgaagtt gcaactgcag ccttttgtgg agctttcgaa
      781 tttcaaggac aaaaatgttc tgcagcttcc agaatgtatg ttccacaatc actatgggga
      841 aaaattaaaa atgaaatgac agtcctgtct gaggaggtga agatgggaga tgtaagagat
      901 tcaggaaatt ttataaatgc agttattgat gaagcatctt ttgacaacat cgtttcatat
      961 atcgaatatg taaaacagtc aaatgatgct gaaattattt ttggaggaac ttacaataaa
     1021 tctgtcggat attttattgc tcctacattg gtgcaaacta cagatcctca ttttaagagc
     1081 atggaagaag aaatatttgg tccattcctt accgtttatg tttacgatga cgataaactt
     1141 gaggaagctg cggaattgtg caatacaaca tctccatatg gtttgaccgg ttctgttttt
     1201 ggtaaagaca gaatagaagt tatgaaattg tgtagcaaat taacctattc tgcagggaac
     1261 ttctacataa atgacaaacc aaccggagct gtagtaggta aacaaccatt tggaggctcg
     1321 agagcttcag gaaccaacga caaggcaggc agcttattta atcttatcag atggataagt
     1381 cccagagcaa taaaagagac tcttgtacca ccaacagata ttttttatga ttacatgaag
     1441 taatttgatt tttacagatc aaactgatct tgttttggga atagtccaaa aagacctccg
     1501 gtatgtataa acaggacatt tccggggtct tttaatttta agtttctcac ttcactgaaa
     1561 aggcctctca tagctttgcc tgtatagaca ggatctaaaa taacaccctc ttttctggca
     1621 aacatggtaa tgaaatcaag ttcttcctta gtacttagtg catatccaat gcctatatat
     1681 ttgtcattaa tttcaatttc atcctttgag atatctacct ctgcatcaag atattcaagt
     1741 gcatcattgc ttatttcatg aaccacattt ttaaaataga cagcgtcatc acatacggcc
     1801 atcccaataa tccttttgcc atagccatga attttattag caagataaag gccagcatat
     1861 gttcctcctg aaccagttgc cacaacaatc gtgttaaaat ttattcccat ttctttttcc
     1921 tgaagtacaa tctcctccat agcagagtaa tatccgagag ctccaatacc aaatgaggca
     1981 ccttcaggaa tgatataagc tctcatcccc ttaaaagcat attcagaggc tatatttttc
     2041 attatttcag ctctagattc tttatattcc tctgaagtac agaaacgtac atctgcacct
     2101 agtagtttgt caaggaagta gtttccttct actttgggaa cttcatctgt tcttagcaaa
     2161 acgacagact ttagaccgat ttttgttgct gaggcaactg ttgctcttgc atgattagac
     2221 tgaatgcctc cacaagtaat aactacatca taattaagat caacaacctc tttaagtaca
     2281 tattcaagtt ttcttatctt attgcctgaa aactcagttc ctgtttgatc atctcttttg
     2341 atatagagat ttatgcctaa atcctctgaa aatctattca gcttttcaat tttagttggc
     2401 agatttgcta agtttaattt tgatttcata acctcttatt gttattctac aaagttatgg
     2461 atttaataat tatttttgcc tgataaaatg acgaaaaaac atgaaaaggt ctttagtttt
     2521 gttgattctg acagtctctt ttattttacc ttttaagtcg gctgctcaaa ttcaagaaaa
     2581 gagtaaattt aaattgattg cagattctgt caatgtatat ttaaagccat tggcaaaagt
     2641 taaaggaata attgagatta aaagtgttaa cctttcaaga aaaagaagat tggatatcac
     2701 ttttaactct acattaggag aatatcctct ccgggaagaa cagatagaga caatttattc
     2761 tattgttaat actttattgc cacctgaata tagagataat aaaataggca ttttttgcaa
     2821 tggatccaaa attgaagaat tatttccttc atttttaaat cctgatcaaa ataaatccgg
     2881 taaggctgag agaaaagtga aacgagataa aggtaagcct aaaacctcca acctgataac
     2941 gaaaggatca ctgccttatg atataagtaa gggtcttcaa aacagacata ttgctatgtg
     3001 gcaaagtcat ggttactact atgaacagaa acttttaaga tgggaatggc aaagagccag
     3061 aatctttgaa actgtagaag atctttatac gcaatcttat gttctcccat ttcttgtgcc
     3121 aatgctggaa aatgccggtg ctactgttat gctgccaaga gagagagata ctcagcttaa
     3181 tgaggtcata gcagataatg atgattctga ttcaggttat tcggaaacaa acgggaatag
     3241 catttgggtt acttcatctg ttccgggatt caaaaatgct aaggagaaat acattcaagg
     3301 agaaaaccca ttcatgatgg gaaccgcccg tctggttcat tttagaaaaa agggagagag
     3361 tgctggtgca aaatattctc ctttttttcc tgaaaatgga gaatatgccg tatatgtagc
     3421 atatcaatca ttccctcaaa gcactgaacg ggctgtttat actgttcacc acaaaggagg
     3481 tgataccaga ttttatgtta atcagaaaat gggtggaggc acttggattt atcttggtac
     3541 attccttttt gaaaaaggga gaaattggga tggttgtgtt gaaatctata acgtttcatc
     3601 acataaagat gaaattgtat ctactgatgc cataaaattt ggcggaggaa tgggaaatat
     3661 agcaagaagc cctgcttccc aaggctcaca aaccaatgtt ccgagttcat ctcatgaaac
     3721 agccaaaaca gttgtcatgc caaatgatat tatacctgaa ggcagtggat atccaaggtt
     3781 tacagaagga gcaagatact ggttgcagtg ggcaggattt aacgatacaa tttactctcc
     3841 taatgctgga gcgaatgact acaacgatga ttatatgtca cgaggaagat gggtaaatgt
     3901 tctttcagga ggttcatctc aaaatcctgt tgaaaaggga tacaatattc ctattgatct
     3961 ctcatttgca tttcattctg acgcaggcac aactcttaac gattcaatca ttggaacatt
     4021 agcaatttac acaagacttt ccaatggcga ttctgttttc cctacaggaa ctccaagaat
     4081 tactagccgg tatcttgcag acatggtaca gactcaaatt gttaatgacg ttagagcatt
     4141 atatgagcct atttgggaaa gaaggggcct ctgggaccgc tcctattcag agtcaagaac
     4201 gccaaaagtt ccatcaatgt tgcttgaact tctttcacac caaaatcttg cagatatgcg
     4261 ttacggactt gacccaacct ttagatttac agtcagtaga gctgtataca aaggaatttt
     4321 aaaatatctg agtctttctc aaaatcgaga atatgttgtt caacctcttc ctgttaagaa
     4381 cttttcggca gaaatctctg ataataacat agttaaactc aactggagtg ctgtagctga
     4441 ttctcttgag tcaactgctg ttcctacagg ctacgtggtt tatagaagaa ttaataatgg
     4501 aggttttgac aacggcaggc ttgttaatga tacacttttt tctgaaaaaa ttgaaacagg
     4561 taaaatattc agttacaaga tcagtgcgat aaatgatggt ggagaaagtt ttccttcaga
     4621 aattctaagt gtatacaaag ctcctgacga aaaaggtaaa gtgttaataa taaattgttt
     4681 tgacagacta agtgcacctg caagttttgc aaccaaagat actacgagag gtggtttcac
     4741 agactttatt gatcacggtg taccttacat aaaagatata tcatatatag gaagtcagta
     4801 tgaatttcgc aggcaaatcc cttggataga tgatgattca ccaggatttg gagcgtctta
     4861 tgccgactac gaaaaaagat taattgccgg taatacattt gattatccat atgttcatgg
     4921 caaagctctt gcatcattgg gctatagttt tgtatcttgc agcagagatg cattaattgc
     4981 agggaaagtt gttcttaacg aatatccaat tgccgacttt atatttggaa agcagttgca
     5041 aacaaaagcc ggtcgaggag tcaaacctgt aaaatattct gtttttccaa agcaacttca
     5101 ggtattaatt aaagattatg cagtccaagg tggtaaatta attgtgtcag gagctaatat
     5161 tgcaactgat atatgggaca gtattgaaat ggaccaggaa agtatggatt ttgcacaaaa
     5221 tgtgttaaaa tacaagtgga gaactaattg tgcaagtaaa acaggagaag tcagggctgt
     5281 tcaaagtcca tataaatttg acggacaata cttattccat actactccaa atgagtatgt
     5341 atactgtgcc gaaagtcctg acggattgga acctgttgga gaaaactcat ggactgtatt
     5401 tagatatggt gataataata tctctgccgg cgttgcctac agaggatcat acagctctgt
     5461 agttcttggt tttccgatcg agacattaac tactgatgaa caaattaaga acttgtttcg
     5521 gactatatta aaatttttga aaggtgagaa ttagtaaaaa cagagaatta gaaattattg
     5581 aacttattaa aagagaggtt aaaccggcac taggatgcac tgaaccaatt gcagtttcac
     5641 tagcagttgc aaaagcagct gaaattttga gggaaagagg ttttgaaccg gaaagtgttg
     5701 tggtagaagt tagtgctaac attcttaaaa acgggatggg tgttggaatt cctggaacag
     5761 ggatggtcgg acttcacatt gcagctgctc tgggaataac ctgcgggaaa tctgcatata
     5821 agctcgaggt attgcaagat ataaatgaag gagcaattga aagagcaaaa aaaatgatat
     5881 ctgaagagaa agtcgatata attcttgcaa aaacaagaaa aaaacttcac atatctgcta
     5941 aatgcatttg caatgaccac tcggccacag tagttattga aaatcagcac gattcgatag
     6001 tttatgctaa ctatgacaat caaataatat ttgacaacag acacaattcc tctgctaata
     6061 caaaattcgg agaatctcaa ataagggata ctcttgacta taaattggat gttgctacta
     6121 tttatcaatt tgccaaacag gttgatttta aagatattga atttattctt gattctgtaa
     6181 ctcttaataa ggcacttgca gttgaaggtc tcaaaaacaa ctatggctta aaagttggta
     6241 aaaccatttt ggacaatatt cacaaaaata tatttggtga aggacttttg acgtattcaa
     6301 tggcaatgac agctgcagct tctgatgcaa gaatggccgg ctgtacttta cctgccatga
     6361 gcaattccgg tagtggaaat caaggcatta ctgcaacaat gccagtgatc tctgcttcag
     6421 aaaaatttgg atcaaatagt gaagaacttg caagagcatt ggttctaagt catttgatag
     6481 caattcatat taagggatat cttggaagac tctcagcttt gtgtggttgt gtgatagcat
     6541 ctacaggagc tgcatgtggc attgtttttc ttaataatgg aacatatgag cagatttgtt
     6601 atgcaattaa gaacatgatt ggaaatatta ccggtatggt ttgtgacgga gcaaaagttg
     6661 gctgtgcact taaagttgct tcaggtgtat cttcggcggt tcagtccgct attctggccc
     6721 ttgaaggcac atgtatctcc gaaaatgacg gaattattga aaaaaacatt gaaaagacta
     6781 ttaaaaatct tgggaaaatt ggatcaatag gcatgcaaaa tacagataat atgatattag
     6841 atatcatggt ctgtaaataa atatgtctat ttgtcagatt ttgtcatttt cacgttattg
     6901 gtatagcttt tgattttgtg gaataaagtt aaaagtaatc aaaattaaaa gtgaatatca
     6961 tgcaaaaggg tacaattgga gttactaccg agaatatatt tccggtaatt aagaagtttc
     7021 tgtattcaga ccacgaaatt tttcttagag aactggtttc aaatgctgct gatgcaacac
     7081 agaaattaaa agcattaatt agcagcggag agtttaaagg tgatgccgga gatcttaaaa
     7141 ttgtaattac atcagacccg gaaaaaggta taattaccgt tagtgacaga ggtgtcggca
     7201 tgacagaaga tgaggttaac agatacatta atcagattgc attttcaagt gccggcgagt
     7261 ttctcgacaa gtataaagat caaattaaca acattatcgg gcattttggc ttgggttttt
     7321 actcttgctt tatggttgca aaaaaggttg aaatccagac attgtcatgg aaagagggag
     7381 ctgaacctgt taaatggagc tgtgagggta ctcctgaata ccaaataacc aaggggaaaa
     7441 gaaaagatag aggaacagac ataatccttc accttgatga tgaatccaaa gagtttgcaa
     7501 cagaaaacaa aataaaagaa ttactgtata agtactgtag atttcttcct attgaaattg
     7561 tctatggcaa agaagagaag ataatcaaca acactaatcc tatatggaca agaaaacctg
     7621 ctgaacttaa agacgaggat tatattgaat tttatcatga actttatcca atgctggagg
     7681 atcctctttt ttacattcat ctaaatgttg attatccgtt ccatttgaca ggggttctct
     7741 attttccaaa actaaaagac aatctcgata tttcaagaaa taaaattcaa ttatactgca
     7801 atcaggtctt cgtgacagac tcagtagaaa acatagtgcc tgattttctg actcttcttc
     7861 atggagtaat cgactcgcca gatattccgc taaatgtttc aagaagttat cttcaatctg
     7921 attcaaatgt taagaaaata tcctctcata taactaagaa agtcgcagac agacttgaag
     7981 agatatttaa gaatgacagg aaaaattttg aagataaatg ggataacttg aaacttttta
     8041 ttgaatatgg aatgctttct gaggaaaaat tttatgaaag agctgaaaag tttgctttat
     8101 tcaaaaactg tgatgataaa tactttagct acgaagaata tagaaaattt atagaatctt
     8161 cacaaacaga caaaaacaaa agactcatat atctttatac tacaaataaa gaggaacagt
     8221 atccctatat tgaggcagct agaaacaaag gatacgacgt attagtattt gataaccaac
     8281 ttgatgcgca ttttatcaat cttcttgaaa gtaaatttaa agactcttca tttgtgagag
     8341 ttgatgccga tcatattgac aaactgattc agaaaaatga gatttcaaaa cccgatcttt
     8401 cagaaaaaga ggttaatgac atatcaactg cttttgaaac agtacttcca aaaggctatc
     8461 actataattt ctcagttgaa aatcttggag aaaatgatgg agctttgctc cttacacgat
     8521 ctgagtttat gagaagatac agagagatgg ctgcgctgaa tggtggcatg aatttctata
     8581 gttctattcc tgaatcttat aacgttatca tcaattatca acatcctctt gtaaaaagag
     8641 ttattgaaat gatgaaccgg gagttgtctt ccaaacttaa agatctggat gaaaaagcgg
     8701 ctgttcatga taaaaggctt tcaacaattc gtgaggaaac atcaggcaaa aaagatgaag
     8761 aaatcgacac tgttgtcaaa gatgagcgca aagctcttga agaagaactc aataagatta
     8821 aagacgaaag gaaagaattg attgccgatt ttgcaaaaaa atgtgaattg atcaaagagc
     8881 tggcagatct tgcattactt gccaatggaa cacttaaagg agaggatctt gataaattca
     8941 taaagagaag tctctcttta attaaatagg ctctgttaag gttctgttac attttttctc
     9001 tttaacaata aatttaattt tcactctgta tttttgaagg aaatttacac gtatgaaaat
     9061 tgacagattt cttcaattat ttattgttaa agagaaaaaa ttttatccca tgttcatctc
     9121 ccagatggat attattaatc aggcaacaaa ggaactcaaa tttctctttt tagaaagcaa
     9181 ctatgacgct caaaaggagt tgta
//
LOCUS       DAOTQL010000062         9131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6655_length_9131_cov_4.795945, whole genome shotgun sequence.
ACCESSION   DAOTQL010000062 DAOTQL010000000
VERSION     DAOTQL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_9131_cov_4.795945"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="P5293_04835"
     CDS             complement(<1..942)
                     /locus_tag="P5293_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR49260.1"
                     /translation="MKKSYKIINDPVHGFINIPSGLIQEIIEHPYFQRLRDIRQLGMT
                     NLVYPGATHSRLLHALGAMHLMKEAIDALRSKGIEIDASEEEASMCAILLHDIGHGPF
                     SHALENNLLVGISHEEISLAMMKAINKEKNNALAKTIEIFEDKYPKHFLHQLVSSQLD
                     VDRLDYLRRDSFFSGVAEGMIGHERIIKMLNVHENSLVVEEKGIYSVEKFLISRRLMF
                     WQVYLHKTVIAAEQLLIQILRRARELTQSSVQLQGSSAIFFFMRNKFSEGDLDDRSII
                     DKFAMLDDNDFISTIKQWHYCDDKILSLLSRNLSMRRL"
     gene            1061..1531
                     /gene="rimP"
                     /locus_tag="P5293_04840"
     CDS             1061..1531
                     /gene="rimP"
                     /locus_tag="P5293_04840"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HRR49261.1"
                     /translation="MIKKETIAALIENYLKDNSLFLVDINISLDNDVEVTVESYFSDV
                     SLDNCTDITKIITDKFNRDVEDFSLTVTSAGLDQPFRILPQYLKYLGKEVSVQLKNGN
                     KITGILNEATDNYIDISYSQTIAIDGKKKREKKEIKEKILLSDIKSTKPFINFR"
     gene            1551..2786
                     /gene="nusA"
                     /locus_tag="P5293_04845"
     CDS             1551..2786
                     /gene="nusA"
                     /locus_tag="P5293_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRR49262.1"
                     /translation="MEGLNLVSTFAEFKELKNIDRPTMMSVLEDVFRNQLIKMYGTAD
                     NFDIIINIDKGDFEIWRNREVVEVVEDPNTQISLEEVSKIDDSYEIGEQFTEEVKLES
                     FGRRGILNLRQNLSGRIMDIEKANLYNNYKDRIGEIIVGEVYQAWRKEVLVMDEEGNE
                     LLLPKSEQIPSDFFRKGDSIRAVVSKVEMENNKPVITLSRTSPVFLERLFEQEVPEIF
                     DGLITIKKIVRIPGERAKVAVESYDERIDPVGACVGVKGSRIHGIVRELRNENIDIIN
                     WTSNLQLLVQRALSPAKISLIKINEQEKRISVYLKPNDVSLAIGKGGSNIKLASMLIG
                     MDIDVFRDLEDEDEEDVLLTAFSDEIDEWIISALKNIGCDTAKSVLALPVSEIVKRAD
                     LDEETVNEVQRILRAEFEE"
     gene            2808..5696
                     /gene="infB"
                     /locus_tag="P5293_04850"
     CDS             2808..5696
                     /gene="infB"
                     /locus_tag="P5293_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRR49263.1"
                     /translation="MTADENIRVNRILKDFNIGMGTLVEFLKTKKIEIDPKPTTRVTP
                     EIYELVRKEFGQEQFIKEQSKKVAIKVKDITERAESSKVEDNDDYVPVKEVFIKTTIV
                     EPPVTPKIVGKIDLDQLNRHSSHTDSHTDIHTPQLEKAHTDTKETKPVTTPEPESPVT
                     EELKKEEPHDNVTTENAPSVDESEKDTSPEAVRQDDEYKDKAAIVEEKKEKSEKKEDR
                     VKTEEKVSEINKKEEPIHIETKVEKLSGLKINGSIDLSQFEKKKPDQKQNSHFNPNKG
                     KRERIHKSGKEKVDLSKESKGTEFKQKGKPGNITQKNPKFQRNDRFKPVKNEVDEDEV
                     QRQIKETYAKMIESKGKTKGSKYRRDKREIASQRHMEEQELQELSNNILRVTEFVTVN
                     DLSVMMDVPVTKVIEACMNLGLMVSINQRLDAEAMVLVAEEFGYKVEFITAEIQEAIH
                     EEEDDEMNLVTRPPIVTVMGHVDHGKTSLLDYIRKSNVIAGEAGGITQHIGAYNVKLS
                     DGQRITFLDTPGHEAFTAMRARGAKITDMAIIIIAADDAVMPQTKEAINHAQAAGVPM
                     IFAINKIDKPGANPDKIREQLAAMNLLVEDWGGKYQCQEISAKKGLNVDKLLEKILLE
                     AELLDLKANPNKRAHGTVIESSLDKGRGYLATVLVQSGTLHVGDVILSGSYTGRVKAM
                     FNERGQKVNEAGPSTPVSLLGLNGAPAAGETFNVMEDEKEARELANKREQLLRIQGLK
                     TQKHITLEEIGRRIAVGNFQELNIIVKGDVDGSIEALSDSLIKLSTEEVHVNVIHKAV
                     GAISESDVLLAAASNAIIIGFQVRPSANARKLAEKEEIEIRLYSIIYDAINEVKSGIE
                     GMLAPEEKEEVIGTAEVRDVFKISKVGTVAGCMVKDGKITRNNRIRVIRDGIVVHEGT
                     LGSLKRFKDDVKEVSAGFDCGLNVDGYNDIKIGDVIEAYTIVEIKRKL"
     gene            complement(5709..6719)
                     /gene="murB"
                     /locus_tag="P5293_04855"
     CDS             complement(5709..6719)
                     /gene="murB"
                     /locus_tag="P5293_04855"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRR49264.1"
                     /translation="MKIVRECSLKDKNTFGFDVKADFYAEVESKEELKLLLSNSEYSQ
                     IPKIVIGSGSNILFRRDFKGIVIHPAIMDISIVGNDCDYIYLRVGAGVVWDDLVQFCV
                     DRGWGGVENLSAIPGCTGAAPVQNIGAYGAEVSNVIHAVEFMFYNDLSAREFSCVECN
                     FGYRDSIFKRELKGCGIITYVTLRLTKNPVINANYEDVKHALHGLDNPSIHTVRDIIS
                     SIRASKLPDPSVVGNAGSFFKNPVVSEKLALSIKNSHPSLKLFPSGEKYYKIPAAWLI
                     EQCGFKGKRVGNVGVHAKQPLVLLAFEGATGDELINLSHTIIQTVKERFNVDIEPEVN
                     IC"
     gene            complement(6697..7959)
                     /locus_tag="P5293_04860"
     CDS             complement(6697..7959)
                     /locus_tag="P5293_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49265.1"
                     /translation="MRFLRGFSRILLGLTFIFSGFTKIIDPVGVGLIFAEYFKIIGLA
                     AAPVICQVMGIVLSSVELLLGISLLLGIRMKVITSVTLILVSFFTIITFFLAIFNPIH
                     DCGCFGEAVKLTNWQSFYKNIILFICALIIYRQRDIFVPIAPTHWEWMLVSLYALAVV
                     TVSVRCYRHLPLIDFTDYKAGKDLRENFILQKNENQPVFETVLIYKKGNTIKEFSIEN
                     LPDSTWTFVDSRSKKVNNTAEPKSGTFAISDRNGEYITDSILSYKGALFITSIPYLER
                     LDQKTIKKIVERDTHFKSMGIKHIILTGSSFESVDEIFGNIDNLVVYYSDYKTLITLN
                     RSNGGLVYLYDGQISAKWSMIDADRLNIESIIKEDPEIISAKKIIKERVTVEVFIALL
                     LLVIFLMRFILKMTYKPNLKKDEDCSRV"
     gene            complement(7961..8638)
                     /gene="tmk"
                     /locus_tag="P5293_04865"
     CDS             complement(7961..8638)
                     /gene="tmk"
                     /locus_tag="P5293_04865"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRR49266.1"
                     /translation="MLIVLEGLDGAGKSTQMYMIENYFKKMGRTVRLLHFPRFDAPVF
                     GDLISRFLRGDLGKIDEVHPLLVALLFAGDRHDAAPLIKGWLEKGDIVILDRYVYSNI
                     AFQCAKAHDQHEEEMVRDWIIDTEFRHFAIPKPDLNLFLDVPIDFIFQKLNEVRVGAE
                     RKYLQGKTDIHEADIFFQKRVRDIYLAQCKLDSSFIRIDCSGVSGEMLDAGSIFELIL
                     KQIEKKC"
     gene            8687..>9131
                     /locus_tag="P5293_04870"
     CDS             8687..>9131
                     /locus_tag="P5293_04870"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRR49267.1"
                     /translation="MIKINYPSVMGIINLNPDSFFSESRCTTENEIISKFEKMIEEEA
                     DIIDLGACSTRPGLRSIPEELEWKYLQPGLKILLKYFPQCTVSIDTFRASIVERAYDF
                     IGDFIVNDISAGEDDPEMLDMVAKLELPYIAMHKKGTPETMQSLCQ"
BASE COUNT         3226 a         1678 c         1677 g         2550 t
ORIGIN      
        1 caatcgacgc attgatagat ttctactcaa tagcgacaaa attttgtcat cacaatagtg
       61 ccactgttta atagttgaaa tgaaatcatt atcgtctaac attgcgaatt tgtctattat
      121 tgaacgatca tcaagatcac cttcggaaaa tttgtttctc atgaaaaaaa agattgcaga
      181 agagccttga agttgaacag aactttgcgt aagctccctt gctctgcgaa gaatctgtat
      241 taataactgt tctgcggcta ttacagtttt atgcaaataa acctgccaaa acattagacg
      301 ccttgaaata agaaactttt ctaccgaata aatacctttc tcctcaacca caagcgaatt
      361 ttcatgaaca ttcagcattt ttattattct ctcatgacca atcatcccct ccgcaacacc
      421 cgaaaaaaaa ctatctcttc tcaaataatc cagcctgtcg acatcaagct gacttgaaac
      481 aagttggtga agaaagtgtt ttggatactt atcttcaaaa atttcaatag tcttggccaa
      541 agcattattt ttctctttat tgatggcctt catcatagca agagaaatct cctcatgtga
      601 aataccaacc agcaagttgt tttcaagtgc atgactgaaa gggccatgac caatatcatg
      661 cagcaaaatt gcacacatgg aagcctcctc ttctgaagca tcaatctcta tccccttact
      721 tctcaatgca tcgattgcct ctttcattag atgcattgcc cccaaagcat gcaaaagtct
      781 tgaatgagta gcaccgggat agacaagatt agtcatccca agttgtctta tatctctaag
      841 tcgctgaaaa taaggatgct caataatctc ctgaattaac cctgatggaa tgtttatgaa
      901 accatgcaca ggatcattaa tgattttata actttttttc atagcgtaaa gttatgaaaa
      961 taaaaaaata ttttgttgtt aataataaaa tttctaaatt tgcatcggtt aagtgaaaag
     1021 atgtaagggg acgaaagtcc ccttattttg tacaattttt atgataaaaa aagaaactat
     1081 agctgctctt attgaaaatt acttgaaaga caattcattg ttccttgttg atattaacat
     1141 aagcctggat aatgatgttg aggtaactgt tgagtcttat ttctctgatg tatctttaga
     1201 caattgcaca gacataacca aaattattac tgataaattt aacagagatg ttgaagattt
     1261 ctctctcact gtcacttctg cgggtctgga ccaacctttc agaatattgc cacagtacct
     1321 caaatatttg ggaaaggaag tctctgtcca actaaaaaat ggaaataaga taacagggat
     1381 acttaacgaa gctactgaca attatataga tatatcctat agtcagacaa ttgccattga
     1441 tggcaaaaaa aaacgtgaaa aaaaagagat taaggaaaaa attttgttgt cagacattaa
     1501 atctacaaaa ccatttataa attttagata attaaaagca aaaaatctat atggaagggt
     1561 taaatctagt aagcacattt gctgaattta aagagttgaa aaatatagac cgcccaacaa
     1621 tgatgagcgt tttggaagac gtttttcgta atcaactcat caaaatgtac ggcactgctg
     1681 acaattttga tataatcatt aacattgaca aaggtgattt tgaaatatgg agaaatagag
     1741 aggttgttga ggttgttgag gatcctaaca cacaaatttc tcttgaagaa gtttccaaaa
     1801 tcgatgattc ctatgaaatt ggagaacagt ttacagaaga agtaaaacta gaaagttttg
     1861 gaagaagagg aatactcaat cttcgccaga atctttcagg aagaataatg gatatagaaa
     1921 aagccaatct ttacaacaac tataaggaca gaataggaga gataatagtt ggagaagtgt
     1981 atcaggcatg gagaaaagaa gttctggtta tggatgaaga aggtaatgaa cttttactgc
     2041 caaaatcaga acaaatacca tctgactttt tcaggaaagg tgactctata agagctgtgg
     2101 tttctaaagt tgagatggaa aacaacaagc ctgttattac tttatctaga acatctcctg
     2161 tatttcttga gagacttttt gaacaggaag ttcccgaaat tttcgatgga cttataacta
     2221 taaaaaaaat tgtcaggata cctggtgaaa gagctaaagt ggctgttgag tcatacgacg
     2281 aaaggattga cccagttgga gcatgcgttg gtgtcaaggg ttccagaata catggaattg
     2341 taagagagct aagaaatgaa aacatagata taataaactg gacatcaaat ttacaactgc
     2401 tcgtacagag agctttgagt ccggctaaaa tttcgttaat aaaaatcaat gagcaagaga
     2461 agcggatcag cgtctacctt aaaccgaatg atgtctctct cgctatcggc aagggtggaa
     2521 gtaacatcaa acttgccagc atgcttattg gtatggatat cgacgtattc agggatcttg
     2581 aagacgagga tgaggaagat gtgcttttaa ctgctttcag cgatgaaata gatgaatgga
     2641 ttatttctgc attaaaaaat attggatgtg atactgcgaa aagtgttctt gcccttccgg
     2701 tctcagaaat agttaaaagg gcagatcttg atgaagagac cgtaaatgaa gtacaaagaa
     2761 tacttcgagc agaatttgaa gagtaatttt taacaggagg ataatgaatg acagctgatg
     2821 aaaacataag agttaaccgt atacttaagg acttcaatat tggtatggga acacttgttg
     2881 aatttctcaa aaccaaaaaa attgaaattg atccaaaacc aacaaccaga gtgactccag
     2941 aaatttatga attagttcgt aaagagttcg gtcaggaaca atttattaaa gaacagtcaa
     3001 aaaaagttgc aataaaggtt aaggatatta ccgaaagagc cgagtcatca aaagtcgaag
     3061 ataatgatga ctacgttcct gtcaaagaag tctttattaa gacgacaatt gttgaacctc
     3121 cagttactcc aaaaattgtt ggtaagattg atctggatca gctcaacaga cactcttctc
     3181 atacagacag tcacactgat atacatacac cccaacttga aaaagcacat acagatacaa
     3241 aagagaccaa gcctgtcaca actcctgaac cagaatcacc tgttaccgaa gagttaaaaa
     3301 aagaagagcc acatgataat gtaacaactg aaaatgcgcc ttctgtcgat gagtctgaga
     3361 aagatacttc tcctgaggct gtacggcaag atgatgaata taaggataaa gcagcaattg
     3421 tggaagaaaa aaaagaaaag agtgaaaaga aagaggatag agtgaaaact gaagaaaaag
     3481 tgagcgaaat caataaaaaa gaggagccta ttcacataga gactaaggta gagaaacttt
     3541 cagggcttaa gataaacggt tctatagatc tttctcaatt tgagaagaaa aaacccgatc
     3601 agaaacaaaa ttctcacttt aatccaaata aaggtaaaag agaaagaatc cacaaaagtg
     3661 gaaaagagaa agtagatctg tcaaaagagt caaaaggaac agaatttaaa cagaaaggga
     3721 aacccgggaa cattacacag aaaaatccaa aatttcaacg taatgacaga tttaaacctg
     3781 ttaaaaatga agttgatgaa gacgaagttc aaagacagat aaaggagact tatgccaaga
     3841 tgatcgaaag caagggaaag actaaaggat ccaagtatcg tcgtgataag cgtgaaatag
     3901 ccagccaaag acatatggag gaacaggaac ttcaggagtt gtcaaataat attttgagag
     3961 taactgaatt tgtaaccgtt aacgacttat ctgtaatgat ggatgtgcct gtcacaaaag
     4021 tgatagaagc atgtatgaat cttggactga tggtttctat aaaccaacgt cttgatgccg
     4081 aagccatggt tctcgttgct gaagaattcg gatacaaagt cgagtttatt actgccgaga
     4141 tacaggaagc gattcatgaa gaagaggatg acgaaatgaa tcttgtcact cgaccaccaa
     4201 ttgtaacagt aatgggacat gtcgatcatg gcaaaacatc attacttgac tacatcagaa
     4261 aatcaaatgt aattgcaggt gaagctggag gcattaccca acatattgga gcttacaatg
     4321 ttaaactgtc tgatggacaa agaataacct ttcttgacac tccgggtcat gaagctttta
     4381 ctgccatgcg agctcgtgga gccaaaataa cagacatggc aataattata attgcagccg
     4441 atgatgctgt catgccccag acaaaagaag caatcaatca tgcccaagca gcaggtgtac
     4501 ctatgatctt tgccatcaat aaaattgata aacctggagc taatcccgac aaaataagag
     4561 aacaactggc agcaatgaat cttttggttg aagattgggg aggaaaatat cagtgtcagg
     4621 aaatctctgc caaaaaaggg ctcaatgttg acaaattact tgagaaaata cttcttgaag
     4681 cagaactgct cgaccttaag gcaaatccca ataaaagagc tcatggaaca gtgattgagt
     4741 cttcacttga caagggacgc ggctatttgg cgactgtcct tgttcaatca ggaacgcttc
     4801 atgtcggtga tgttatcttg agtggaagtt ataccggaag ggttaaggca atgttcaatg
     4861 aaagaggcca aaaagtaaat gaagcaggcc cgtcaacgcc tgtctcactg cttggattaa
     4921 atggtgctcc tgcagccggt gaaacattca atgtaatgga agatgaaaag gaggcaagag
     4981 aactggccaa taaaagagaa caactactga ggattcaggg acttaaaaca caaaaacaca
     5041 taactctcga agaaattggc agacgaattg cagttggcaa cttccaggaa cttaacatca
     5101 ttgttaaagg agacgtagat ggttcaatcg aggctctctc cgattctctc attaaactat
     5161 ctacagagga ggtccatgta aatgttattc acaaagccgt aggagctatt tcggaaagtg
     5221 atgtgttgct ggctgctgca tctaacgcga taatcatagg atttcaggta agaccttcgg
     5281 ctaatgcaag aaaacttgct gaaaaagaag agatagaaat acgactctac tctataatct
     5341 atgatgccat aaatgaggtt aagagtggta tagaaggtat gcttgctcct gaagagaaag
     5401 aagaagtaat aggcacagca gaggtaaggg acgtttttaa aatttcaaaa gtcggcacag
     5461 tagcaggttg tatggttaaa gacggaaaaa ttacgagaaa caacaggata agagtcatac
     5521 gcgatggcat agttgtacac gaagggactc tcggatcgct caagcgattt aaggatgatg
     5581 tcaaggaagt gtctgccgga tttgactgtg gccttaatgt agacggatac aacgatatta
     5641 agatcggaga tgtaattgag gcatatacaa ttgttgaaat caagagaaaa ctttaatcaa
     5701 taattgattc agcatatatt aacttctggt tcaatatcca cattaaatct ctcctttaca
     5761 gtttggataa ttgtatgtga gagatttatc aattcatccc ctgttgctcc ttcaaaagca
     5821 agcaaaacca acggctgttt tgcatgaaca ccaacgtttc caactctttt ccccttaaaa
     5881 ccacactgct ctatcaacca tgcagcagga attttataat atttctctcc tgaaggaaaa
     5941 agctttagtg aaggatgact atttttgata gagagagcca gcttttcact aacaaccgga
     6001 tttttaaaaa aacttccagc attgccgaca actgaaggat ctggtaactt actagctctt
     6061 atagaagata ttatatctct gacagtatga atggaaggat tgtccaaacc atgcaaagcg
     6121 tgttttacat cctcataatt agcgttgatt acaggattct tagtcaatct gagtgtaaca
     6181 tatgttataa ttccacaccc tttcaattct cgcttaaaaa tgctatctct gtaaccaaaa
     6241 ttacactcta cacaagaaaa ttcccttgca gaaagatcat tgtaaaacat gaattccacc
     6301 gcatgtatta catttgaaac ttcagcccca taagctccaa tgttttgaac aggagctgcc
     6361 ccggtacatc caggaatagc tgaaagattt tccacacctc cccaccccct gtctacacaa
     6421 aactgcacaa gatcatccca aaccacacct gcaccaactc ttaagtaaat ataatcgcag
     6481 tcattaccga caatagaaat atccattatg gcgggatgta taactatccc tttgaaatct
     6541 cttcgaaaaa gaatatttga tcctgacccg attacgattt tggggatttg ggaatattct
     6601 gagttactca gcaacaattt caattcctcc ttactttcta cctctgcata aaagtcagct
     6661 tttacatcaa agccaaatgt atttttgtcc ttaagactac actctcgaac aatcttcatc
     6721 tttttttaaa tttggtttat atgtcatctt gagaataaat ctcataagaa aaattaccaa
     6781 caaaagaaga gcaataaaaa cttctacagt aacacgctcc ttaataattt tttttgccga
     6841 tattatctct ggatcctcct ttatgattga ttcaatatta agtctatctg catctatcat
     6901 tgaccatttc gcagaaattt gaccatcata gagatatacc aatcccccat ttgaacgatt
     6961 tagagttatg agagttttat agtccgaata ataaacaaca aggttatcaa tatttccgaa
     7021 aatttcatca acactctcaa aagaagagcc ggtgagtata atatgtttaa tacccataga
     7081 tttaaaatga gtatctcttt cgacaatttt tttaatagtc ttttgatcaa gtctttccaa
     7141 ataagggata gaagtaataa aaagagcacc tttgtaagat aagatagaat cagtgatata
     7201 ttcaccattt ctgtcagata ttgcaaaagt tccggatttt ggctctgctg tgttatttac
     7261 cttcttgctt ctcgaatcaa caaatgtcca ggtagaatct ggaaggtttt caatcgaaaa
     7321 ttccttgata gtgttcccct ttttataaat caatactgtt tcaaaaacag gctgattttc
     7381 atttttttgt aaaatgaaat tctctcttag atctttccca gctttataat ccgtaaaatc
     7441 aatcagaggc aaatgccgat agcaacgtac ggaaacggtt acgacagcta atgcatataa
     7501 agaaacaagc atccactccc aatgagtagg agcaattgga acaaatatat ctctctgtct
     7561 gtatattatc agtgcacaga taaataatat aatgttttta taaaaacttt gccagtttgt
     7621 tagtttaact gcttctccaa aacacccgca gtcgtggata gggttaaaaa ttgccagaaa
     7681 aaaagtaatt attgtaaaaa atgagactaa tatcagtgta actgaagtaa ttactttcat
     7741 ccttatacca agtagtaagg aaatacctaa aagcaactct acagatgaca gcactatccc
     7801 catgacctgg cagatcacgg gagcagcagc caaccctata attttgaaat actctgcaaa
     7861 tatcaacccc actcccaccg gatcaattat cttggtaaat cctgaaaaaa tgaaagtcag
     7921 tcctaacaat attctggaaa accctcttaa aaatctcata tcaacatttt ttttcaattt
     7981 gttttaaaat caattcaaat atcgagcctg catcaagcat ctctcctgaa acaccagaac
     8041 aatcaatcct tataaaactg ctgtccaatt tgcattgagc aagatatata tctctgactc
     8101 gtttctgaaa aaaaatgtct gcttcatgaa tatctgtctt gccttgtaaa tattttctct
     8161 ctgctcctac cctcacttca tttaattttt gaaaaataaa gtcaattgga acatctaaaa
     8221 acaagttcag atcaggtttt ggaatagcaa agtgacgaaa ttccgtatcg ataatccaat
     8281 ctctcaccat ctcctcctca tgctggtcat gtgctttggc acactgaaaa gctatatttg
     8341 aatatacata tctgtcaaga attactatat ctcccttttc aagccaaccc ttgatcaaag
     8401 gagcagcgtc atgtctgtcc cctgcaaaca acaaggcaac aagtaatgga tgaacttcat
     8461 caatctttcc caaatctcct ctcaaaaatc gagatatgag atctccaaag acaggtgcat
     8521 caaatctcgg aaaatgaaga agtctgacag tcctgcccat ttttttgaaa taattctcta
     8581 tcatgtacat ctgggtagat ttccctgccc cgtccagtcc ttccagaaca attagcataa
     8641 taagtcctat atttgtaatt aaagcaaagt tagttaataa atagaaatga taaagatcaa
     8701 ttatccgtca gtgatgggta taattaacct caatccggat tcattcttca gtgagagccg
     8761 ttgtactaca gaaaatgaga ttatctcaaa atttgaaaag atgatagaag aggaggccga
     8821 tattattgac ctgggagcat gctctaccag acccggatta agaagtattc ctgaagaact
     8881 cgaatggaaa tatcttcagc caggtttgaa gattctttta aaatattttc ctcagtgcac
     8941 tgtttcaatc gacactttta gggcttcaat tgttgaacgg gcgtatgatt ttattggcga
     9001 ttttatcgta aatgatatct ctgccggaga agatgaccct gaaatgctcg acatggttgc
     9061 aaagcttgaa ttaccataca ttgccatgca caaaaagggc acaccggaaa ctatgcaaag
     9121 cctctgccag t
//
LOCUS       DAOTQL010000063         9130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6657_length_9130_cov_4.703140, whole genome shotgun sequence.
ACCESSION   DAOTQL010000063 DAOTQL010000000
VERSION     DAOTQL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_9130_cov_4.703140"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="P5293_04875"
     CDS             <1..1909
                     /locus_tag="P5293_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49268.1"
                     /translation="INGANGTAWAWGYNSYGQLGCNNRTNYSSPISVVGEKSWAKIFC
                     QANQTIAIDTDGKCYSWGQNNYGQLGDGTTMSKSSPVSVVGDHLFTQLCGNDDHVHGL
                     EGANGTVWSWGQNISNAALGISASAHRSTPTSVVGGHSFIQITCNGSGAAGLKEDGTV
                     WAWGQNSMGEVGDNTRTTRSSPVSVVGDHSFISIAGGCSVLSTNSYYLALKADGTLWG
                     WGFAGDLQFGPSALSFSSPVCIPYFGGDRKIVRMTTGDQHYHCVDTNGRWWGMGRWSQ
                     TRYDQNRVAAVSMPRQTNRPMRVSISLKIVSGNVARSTAVAIANDGSAWSWGSNQYGE
                     LGIGTTISKSSPSSILGGKSFSKIYNASQSMIGIDGSDGSAWSWGYNNYGQLGDNSKT
                     NRSSPVSVVGSISFKDVVIDATCSLGIKGDDGTCWSWGYNSYGQLGDNTTTDRSSPVS
                     VVGGRSAIQIAMNHNSGSFLINGADGTVWAWGQNTGAATGFLGISIAGNQSTPRSVAG
                     GHTFKSICAGDGHCLALKDDGTCWSWGLNSYGQLGDNSITSRSYPVSVRGDHSFVSIR
                     ANSQVSMGIKSDGSIWVWGGDYTYNQLFMNKYEAGGTFGYSSPVAMTSIAYYKYNKGP
                     AHQNNYWDNIW"
     gene            1919..3514
                     /locus_tag="P5293_04880"
     CDS             1919..3514
                     /locus_tag="P5293_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRR49269.1"
                     /translation="MKKAIVTLCVGDSYKLISNYSHPTIKKYADKIGADFIVYNEHIS
                     DPPHYEKLIIIYDLLKKYDRLLWLDTDLIVRDDCPNLFEIVSSKELGIFNEAPYTDRI
                     PALMELNKLYNQNFETGTYYNTGVMVLSKIHRNVFYPVTEIPFIQNSFGEQTFLNYRI
                     LKDDLVKVHKLNYRLNRMSCMDEFTGENRLSSYIVHYAGCPLPALMVELMRKDLEGWE
                     KGKPDYKYKRSIFVQVGGGMGDQLCAEPVLRFIRKWYPKDSNDIYVTTHWPRLFEHLK
                     DDFVVAEKFQTFNDVSLYRMETAPNENQLIWQFISHPLVHATDFASLATLRRTIPDIE
                     KQIKLEVYDHEIEEVKSIYEKPEELILVHPGRGWKSKTFPIEWWLAVINGLINEGYKV
                     GIIGKHIGDDQGYVDVKCPKGAVDFRDMLSIGGLIALISMSPIVVTNDSSPVHIAGAF
                     DNWIVLIPTCKHPDHVLPYRKGSKSYKTLALYKRLTTDDIESLPTTIDGASVDIVPGD
                     ILDYIPEPEEVVKETINIIKREV"
     gene            3517..4086
                     /locus_tag="P5293_04885"
     CDS             3517..4086
                     /locus_tag="P5293_04885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR49270.1"
                     /translation="MITKEKIREMMDDIGIRPDMVPHMPFLALEASKHSFVIELGVEY
                     GNGSTKALAEGLRNSDAEDKLMISIDIEDKMTKYRIPTENWWKFILGDDVEATVIDKV
                     KELANGRKADIIFVDSFHDPDHITKELNAYFPLTDDNTVWYFHDVIDNISGEEQNIVQ
                     PIKDFAAQNNLTYEVVSTKSCGLGKLYKK"
     gene            4159..4326
                     /locus_tag="P5293_04890"
     CDS             4159..4326
                     /locus_tag="P5293_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49271.1"
                     /translation="MNQKRNSILEKRIMMINERLATFEDQVLTPEMEQQKERLLKVKE
                     TLQRFIGKTEE"
     gene            4406..4981
                     /locus_tag="P5293_04895"
     CDS             4406..4981
                     /locus_tag="P5293_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49272.1"
                     /translation="MDSYGFNNQGPFYVQREATLPAWSASDEGRLIYDESADSLYYGT
                     STDWVLVSISSGIVSGVKMYFYQNTAPVGWTIDVSVADALIGVKGGTSSFNVAGGNLA
                     GTWTQPGHTHMTADHTHSVVIPGDSGWPHSNIYGYGQLAVGRGHAEYVDYMTSNRTLT
                     SGSATGGSTTSSGTTNTYRPYAAIGIICTKS"
     gene            4994..5368
                     /locus_tag="P5293_04900"
     CDS             4994..5368
                     /locus_tag="P5293_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49273.1"
                     /translation="MRSHGKRFIGKTEFVFDPTGDEVLEIVTINNINIDLANRTPAST
                     RLTAFDLIVSVHGGIGEITYHMSKNGMSWSSSTNPQLAYLDYTSADIGSTAAYVKVTD
                     ESAQEDYVIVSITLQDNYGRGM"
     gene            5394..7325
                     /locus_tag="P5293_04905"
     CDS             5394..7325
                     /locus_tag="P5293_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49274.1"
                     /translation="MARTKSYGKDFQGKLILEKLATLPTWTADDYGRLVYNEDDDKLY
                     YGTDSDWLEVGTGGGGGSGGYELFTQSTPATAWSITHSLGQKYVTVMVVDDADDNIID
                     PLEIHYIDSDELEITFSSAISGKAILTGGASGAGGTSGTSGSSGISGTSGSSGTSGTS
                     GTSGSSGTSGISAGFQYEFDSTTSDADPGAGKLRFNNATPANVTYLYINGTDDYSNDI
                     TGFWDSIISTLSIYEPSGGGTDDSYWNLWPGLETFSSSSTTIAMGPNWNHSYIKFRSD
                     ETIYKTTKISKAFIRFTAAANGGTTPENHPPRVLIYGTVSAAPTNVATALALTRLQDY
                     VDWRIPEWVLGSTYDTPNIAAPLNCILQNNSHAPTDDFVFILDTYTGGFSTSRTYYTS
                     EYGSNYPVLYILHGDTATTGYLRLSKVGDPNTFVLFEVIGVLSATGYYRAWVTYVDSN
                     GTISNTDRVIVSWVKSGNDGSSGAPGTSGSSGTSGSSGTSGSSGTSGVSGTSGSSGTS
                     GSSGTSGESLALTTIPIFDSDTPCASGAGYTGFVIPLDLNGYELTDIIASVAIPGTGT
                     YEPDMDIQVRRRRAGSEVDMLLTPVTVGSGEYYARDGVIDTSNDDVATGDLIFIDVDT
                     VHDPTPAYGLSVALTFTKA"
     gene            7335..8573
                     /locus_tag="P5293_04910"
     CDS             7335..8573
                     /locus_tag="P5293_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49275.1"
                     /translation="MASVTSTLSFGIEDRYQNIGYVYSSASFNTGSASFGDYNGTYKY
                     DCFIRFGPIYIPQGSIINYAYIRFLGHNSPNLHTCNAIIKVADDPTAPTTHAQYDAKT
                     WSTTTVEWNNVEEFGGSGPDLSAKRNTPSITSLIQEKISEAGWSEGGYILIGIQDNGS
                     DSLVYRRIDGTGDGSQRCPVLVVTFTTENKWYGQAQATTDIISAIRCPSNPSYDDIEV
                     PPSTYSFLPWGASTRFSVPILYDWGLRFPSCNIPRRSIITSASLYQFNRSISQTLDVN
                     YDIYANYINNAISPPNDDEDAWAALVKTTKYYSNVWTTDMVSWTWYLMASGFTEPLQE
                     VINKLGYRQGNAIMLMGFETYVSVPNSYLQITSLGGDSPYYVDKYAYAPKLKIEFTDY
                     TGDPPQVVPLAYSGKDYTIK"
     gene            8586..8915
                     /locus_tag="P5293_04915"
     CDS             8586..8915
                     /locus_tag="P5293_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49276.1"
                     /translation="MSNGEEFSVWSNFIWNFKRLWPDILALLSIFLVLFFIPTEFFLQ
                     ENPKMGLLSIFVGKLMFISAGILHAHITRKIMWNYINFSTETDMVRKLMVVGWYMIII
                     FAWARGG"
BASE COUNT         2867 a         1469 c         2044 g         2750 t
ORIGIN      
        1 tattaatggt gcaaatggta cagcatgggc atgggggtac aattcttacg gacaactcgg
       61 atgtaataac agaacaaatt attcttctcc catatcagta gttggtgaaa aatcttgggc
      121 gaagattttt tgtcaggcaa atcaaacgat agcgatagat acggatggga aatgttatag
      181 ttggggtcaa aataattatg ggcaacttgg agatggtacc acaatgagca aatcatctcc
      241 cgtatctgta gttggtgatc atttatttac acaattatgt ggtaacgatg atcatgttca
      301 tggactcgaa ggagcaaatg gtactgtttg gagttggggt cagaacatca gtaatgcggc
      361 tcttggtata tcagcatccg ctcataggtc aactcctaca tcagtagtcg gtggtcattc
      421 ctttattcag attacatgca acggatcagg tgctgctggt ttgaaagaag atggtacagt
      481 atgggcttgg gggcagaatt ctatgggaga agtgggggat aatacacgca ccacaagatc
      541 atcacccgta tcggttgttg gtgatcattc cttcatttct attgctggtg gatgtagtgt
      601 ccttagtaca aatagttact atcttgcact taaagctgat ggtactttat ggggttgggg
      661 gtttgctggt gatttgcaat ttggtccttc ggcactcagt ttttcatctc ctgtttgcat
      721 accgtacttt ggtggtgata gaaaaattgt gagaatgact accggagatc aacattatca
      781 ttgtgtcgat acaaatggta gatggtgggg tatgggtcgt tggtcgcaga cgaggtatga
      841 tcaaaatagg gttgccgctg taagtatgcc aagacagaca aataggccaa tgagggtatc
      901 tatttcttta aaaattgttt ctggtaatgt agcaagaagc acagctgttg caattgcaaa
      961 tgatggctct gcttggtctt ggggaagtaa tcaatatggt gaacttggta tcgggacaac
     1021 tataagtaaa tcatctccat catctattct tggtggtaaa tcattttcta aaatatataa
     1081 tgcctcacaa agtatgattg gtattgatgg ttctgacggt tctgcttggt cttggggata
     1141 taataattat ggtcaactcg gagataattc aaaaactaat agatcatctc ctgtttctgt
     1201 agtcggctct ataagtttta aagatgtagt tattgatgct acttgtagtc ttggtatcaa
     1261 aggtgacgat ggaacttgct ggtcttgggg atataatagt tatggtcaac tcggagataa
     1321 tacaactaca gatagatcgt cccctgtttc agttgtgggt ggaaggtcgg ctatacagat
     1381 tgcgatgaat cacaattctg ggtcatttct tattaatggt gctgatggga cagtatgggc
     1441 ttgggggcag aatacaggcg cagcaaccgg ttttcttgga ataagtatcg caggaaacca
     1501 gtctactcct cgatctgttg ctggtggcca tacttttaaa agtatatgtg caggcgatgg
     1561 gcattgtttg gcattaaaag atgatggaac ttgctggtct tggggtctga atagttatgg
     1621 gcaacttggt gataatagta taacaagtcg gtcatatcct gtatcagtta ggggtgacca
     1681 ttcgtttgtt tctattcggg caaattcgca agtatcaatg ggaataaaat ctgatggatc
     1741 aatatgggta tggggaggag actatacata taatcaattg tttatgaaca aatatgaagc
     1801 cggaggaaca tttggataca gttctcccgt ggcaatgaca agtattgcat actataaata
     1861 taacaagggc ccagcccatc agaataatta ttgggataat atatggtaag gagaatttat
     1921 gaaaaaagca attgtgacct tatgtgtggg ggatagttat aaactcatat caaattacag
     1981 tcatcctaca atcaaaaaat acgcagataa aattggggca gatttcattg tttataacga
     2041 acatatttct gatccacctc attatgaaaa acttattatc atatatgatt tattgaagaa
     2101 atatgacagg ttgttatggc ttgatactga cctgattgta agggatgact gtcctaattt
     2161 gtttgagatt gtgtcgagta aagaactggg catcttcaat gaagctccct atactgacag
     2221 gatacctgct ttaatggaac tgaataaatt atataatcaa aattttgaga caggcacata
     2281 ttacaacact ggtgtaatgg ttctatcaaa aatacataga aatgtgtttt atcctgtgac
     2341 agaaattcca tttatacaaa actcttttgg tgaacagaca ttcttgaatt acaggatact
     2401 caaagatgat cttgtaaaag ttcataaatt gaattacagg ttaaacagaa tgagttgtat
     2461 ggatgaattt acaggagaaa acagattgtc atcatatatt gttcactatg ccggctgtcc
     2521 attaccagca cttatggttg aattgatgag aaaagatttg gaaggatggg aaaaggggaa
     2581 accagattat aaatacaaga ggtctatttt tgttcaagtt ggcggtggaa tgggtgacca
     2641 actatgcgca gaacccgtgt tgaggtttat aagaaagtgg tatccgaaag attccaatga
     2701 catttatgtg actacacatt ggccaaggtt atttgagcat ctgaaagacg attttgttgt
     2761 tgcagaaaag tttcagacat ttaatgatgt ttcactatac agaatggaaa cagcaccaaa
     2821 tgaaaatcaa ttgatatggc agttcatttc tcacccactg gttcacgcta cggattttgc
     2881 ttctcttgca acattgagaa gaacgattcc tgatattgaa aaacagataa aactggaagt
     2941 atatgaccac gaaatagagg aggtaaaatc aatatacgag aaacctgaag aacttattct
     3001 ggttcatcct ggcagaggat ggaaaagtaa aacattcccc attgaatggt ggctggcagt
     3061 aatcaacggt cttataaatg aggggtataa agttggtatt atcggtaaac atatcggcga
     3121 tgatcaggga tatgttgatg taaaatgtcc aaagggtgca gtggatttca gggatatgtt
     3181 atccatagga ggactgattg cattgatttc aatgtcccct atcgttgtaa cgaatgatag
     3241 ttctcctgtt catattgcgg gggcatttga taattggata gttcttattc caacttgtaa
     3301 acaccccgat catgttcttc cctacagaaa gggatcaaaa tcatataaaa cattggcgct
     3361 ttataaaaga ttgaccacgg atgacataga gagtttaccc actacgattg atggcgcaag
     3421 tgttgatatt gttccgggcg atatccttga ttacattcca gaaccggaag aagtagtcaa
     3481 agaaactata aatataataa aacgggaggt gtgaatatga taacaaaaga aaaaattcga
     3541 gaaatgatgg atgatatcgg tataaggcct gatatggttc ctcatatgcc ttttctcgca
     3601 ttggaagcca gtaaacattc ttttgttatt gaacttggtg tagaatatgg aaatggttcc
     3661 acgaaggctc ttgcagaggg attaagaaat tctgatgcag aggataaact tatgatatca
     3721 attgatattg aggataaaat gaccaagtat cgtattccca ccgaaaactg gtggaagttt
     3781 atcttgggtg atgatgttga agcaactgta attgataagg tgaaggaatt ggcaaacggt
     3841 agaaaagcag atattatttt tgtggattct tttcatgatc cagaccatat tacaaaggaa
     3901 ctcaatgctt attttccact gacagatgat aatacagtct ggtattttca cgatgttata
     3961 gacaatataa gtggagaaga gcagaatatt gttcagccca taaaagactt tgcagcacag
     4021 aataatttga cttatgaggt agtttcaacc aaatcttgtg gtctcggtaa actatacaaa
     4081 aaataataga aaaacatata tctataatca aacccctctg gaggaaacga tagtgatatt
     4141 tgatataagg aggaaaatat gaatcagaaa aggaattcga tattagaaaa aaggataatg
     4201 atgattaatg aaagacttgc aacatttgag gatcaagttc ttacccccga aatggagcaa
     4261 cagaaagaga gacttctgaa agtgaaagag actctacaga gatttatagg caaaacagaa
     4321 gaataatttt cataaatata aacaaattgg attcattgtg agggtggtac ggataaaaaa
     4381 agtaaaaata taagaggaaa aatatatgga ttcctacgga tttaataatc aaggcccatt
     4441 ttatgttcaa agggaagcaa cattaccggc atggtctgca agtgatgaag gtagattgat
     4501 ttatgatgag tctgcggata gtctctatta cggaacatcc actgattggg tattagtgtc
     4561 aatatcttct ggtattgttt ctggagtaaa gatgtatttt tatcagaata cggctccagt
     4621 aggatggaca attgatgttt ctgttgccga tgctcttata ggtgtaaaag gtggaacaag
     4681 ttctttcaat gttgctggtg gaaatttagc gggaacttgg acacaacccg gccatacaca
     4741 tatgacggca gaccatacac attcagtagt aattccgggc gacagcggct ggcctcatag
     4801 taatatatat ggttacggcc aacttgctgt tggccgcggt catgctgaat atgttgatta
     4861 tatgacatct aacagaacat tgacaagtgg ttctgcaacg gggggtagca ccacaagttc
     4921 tggtactacg aatacatacc gcccatatgc tgctatagga attatttgca cgaaatcata
     4981 atgaggaaat aggatgagat cacacgggaa aaggttcata ggtaaaacag agttcgtttt
     5041 tgatccaacg ggagatgagg ttcttgaaat agtcacaata aataatatta atatagacct
     5101 tgctaataga accccggcgt caacgagact tacagccttt gatttgatag tgtctgttca
     5161 tggcgggatt ggtgaaatta catatcatat gagcaagaat ggaatgtctt ggagttcgag
     5221 tacaaaccca cagttggcat atttggatta tacatctgcc gatatcgggt ccaccgcggc
     5281 atatgtgaaa gttactgatg aatctgcgca agaagattat gttatagtat ctataactct
     5341 tcaagacaat tacggtagag ggatgtaaaa atatcgatat agaggatctg taaatggcga
     5401 gaacaaaaag ttacggtaag gattttcaag ggaagctcat tcttgagaaa ttggcaactc
     5461 ttcccacatg gacagccgac gattatggta gacttgttta taatgaagac gatgataaac
     5521 tctattatgg aacggatagt gattggcttg aagtgggaac tggtggcggt ggcggtagtg
     5581 gtggatatga gttgtttaca caatcaacac ccgcaaccgc atggtctatt acacattctc
     5641 ttggtcagaa atatgtcacg gttatggttg tggacgatgc cgatgataat attattgatc
     5701 ctcttgaaat tcactacata gatagtgatg aattggaaat tacttttagt tcagcaattt
     5761 ctggaaaagc aattcttact ggtggggcaa gcggcgctgg tggaacatcc ggtacctctg
     5821 gttcaagtgg aatctctgga acttctggaa gtagtggcac aagtggaact tccggaactt
     5881 caggttcttc tggtacttca ggcatttctg ccggttttca atatgagttt gattctacga
     5941 catctgacgc cgaccccggc gcaggaaaat tgagatttaa taatgcaact cccgcaaatg
     6001 taacatatct ctatattaac ggaactgatg attattcgaa tgatataact ggattttggg
     6061 atagtataat ttcgacatta tcaatttatg agccttccgg tggtggaaca gatgattcat
     6121 attggaattt atggcccggc cttgagactt tctcatcctc aagtacaaca attgcaatgg
     6181 gccctaattg gaaccattcc tacataaaat tcagaagtga tgaaacaatt tataaaacaa
     6241 cgaaaataag taaagcattt ataagattca cagccgcggc gaatggtgga acaacacctg
     6301 agaatcatcc accaagagta ttgatatatg gaacagttag cgcagcacca acaaatgtag
     6361 ctacagcatt agcacttaca agattacaag attatgtaga ttggagaata ccagaatggg
     6421 tattgggttc tacttatgac acaccaaata ttgcagctcc tttgaattgt attttacaga
     6481 ataatagtca tgcaccaaca gatgattttg tttttatctt ggatacatat acggggggat
     6541 tttcgacaag tagaacatat tatacttctg aatatggatc aaattatcca gttctttata
     6601 ttttacatgg agataccgcc acaaccggat atttgagatt gtcaaaagtt ggcgatccga
     6661 atacattcgt tttattcgaa gttatcggtg ttctttctgc cacaggttat tacagagcat
     6721 gggtaacata tgttgatagt aatggtacaa tatcaaatac tgatcgagtt atcgtttctt
     6781 gggtgaagag tggtaatgat ggttcttccg gggctcccgg tacatccggt tcaagtggaa
     6841 cctctggttc ttccggaacc tctggctcaa gtggaacatc tggggtatca ggaacatcag
     6901 gaagttcagg cacaagtggt tcaagtggaa catctggtga atcattggca ctcacaacga
     6961 ttccaatctt tgattccgat actccttgtg catccggtgc tggatatacg ggatttgtca
     7021 ttcctcttga tttgaacggt tatgaattga cagatataat cgcctcagta gccataccgg
     7081 gcacaggaac atatgaaccc gatatggata tacaagtgag gagaaggaga gccggatccg
     7141 aagttgatat gttattaacc cctgtaacgg ttggttctgg tgaatattat gcaagggatg
     7201 gtgtaataga caccagtaat gatgatgtgg ctacgggtga cttgatattc attgatgtgg
     7261 atactgttca cgacccgaca ccagcatacg gattatctgt agccttgaca tttaccaagg
     7321 cataaggaat ttctatggca tctgttactt caacattgag ttttggtatt gaagatcgtt
     7381 atcaaaatat aggctatgta tacagttctg ccagcttcaa cacgggctca gcatcttttg
     7441 gagattataa tggaacatat aaatatgatt gttttataag atttgggccc atttatattc
     7501 cacagggctc tataataaat tatgcgtata taagattttt aggtcataat tctcctaatc
     7561 tgcatacttg taatgcaatt ataaaagtgg cggatgatcc cacggcacca acaacacatg
     7621 cgcaatatga cgcaaaaact tggagtacga ctactgtgga gtggaataat gttgaggaat
     7681 ttggtggtag cgggcccgac ttatcagcaa aaagaaacac tccgtctata acatctctta
     7741 tacaggaaaa aatctccgaa gcaggatggt ctgagggtgg ttatatatta atcggtattc
     7801 aggataatgg gtctgatagt cttgtgtatc gtcgcattga tggtacaggg gatggatcac
     7861 aacggtgtcc tgttttggtt gttacattta caactgaaaa taaatggtat ggtcaggcac
     7921 aggcaacaac tgatattata tctgctatta gatgcccctc aaatccaagc tatgacgata
     7981 ttgaagttcc cccatccacc tactcctttc ttccttgggg agctagcact cgtttttcag
     8041 taccaatttt atatgattgg ggattgaggt ttccaagttg taatattcca agaaggtcta
     8101 taattacatc tgcaagtcta tatcaattca atagatcaat atcacagaca cttgatgtaa
     8161 attatgatat atatgcaaat tatattaata atgcaatttc accacctaat gatgatgagg
     8221 atgcatgggc tgcccttgtc aaaactacca aatattatag taatgtctgg actacagata
     8281 tggtcagttg gacttggtat ctaatggcat ctggttttac agagccactt caagaggtta
     8341 ttaacaagct tggctataga cagggtaatg caattatgct aatgggattt gaaacctatg
     8401 tttcagtacc taatagctat ctgcaaataa catctcttgg tggcgatagt ccatattatg
     8461 tggacaaata tgcttatgca ccaaaattaa aaattgaatt tacagattat acaggtgatc
     8521 cgccacaggt agttcctctg gcttattcag gaaaagatta tacaataaaa taagggaggt
     8581 gttttatgtc aaacggtgaa gagttttcag tgtggagcaa ctttatttgg aacttcaaga
     8641 gattgtggcc agatatctta gctcttcttt caatttttct tgtattgttt tttataccaa
     8701 cagaattttt tcttcaggaa aatccgaaga tgggtttact tagtattttt gtaggtaaac
     8761 ttatgttcat ttctgctggt attcttcatg cacacatcac aagaaagata atgtggaact
     8821 atattaactt cagcacagaa actgatatgg ttagaaagtt gatggttgtt ggttggtata
     8881 tgatcataat ttttgcttgg gcacgcggcg ggtagtccaa tgatttcaga tgtttacatt
     8941 ataaaaaatg ttatcaacag aaggctttag atagagggaa aaatagatga tagactatat
     9001 tttggacaga aaagagaaga aaataagagg tggatttatc aacgctttta taacattcac
     9061 ctttcttttt ctcatagttt atggtataat agatgagaaa atccagaaaa gtatacttgc
     9121 tttgcaatat
//
LOCUS       DAOTQL010000064         9075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6699_length_9075_cov_5.478271, whole genome shotgun sequence.
ACCESSION   DAOTQL010000064 DAOTQL010000000
VERSION     DAOTQL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6699_length_9075_cov_5.478271"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="P5293_04920"
     CDS             <1..217
                     /locus_tag="P5293_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49277.1"
                     /translation="NLYLNSPLFSERAKIFGKIEAAKMFSYQEYRFFRQKVFVDLIFL
                     LSLFPYPYFALRDLEEKNLPYCRYMKV"
     gene            230..1054
                     /locus_tag="P5293_04925"
     CDS             230..1054
                     /locus_tag="P5293_04925"
                     /inference="COORDINATES: protein motif:HMM:NF023645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HRR49278.1"
                     /translation="MKQGEVRKILRDDNVRIGFELECIFPAFFHDKRYKSGGYRYDSK
                     YLFSIAVDYFKVNRLPFKAVFTEEYYYNNKNKWVIKPDNSIGAAGLELVSPVMELEEY
                     LDICPVIFSHMKKVKAVTDNKCGFHLNLSLKDKGWYNRLDVMKLGLLINEREIYDLFP
                     MRKNNTYCESVHKDIKKVLKKKIGEYILENYKLKPEFTESHHMAINISHLKQKNKYIE
                     FRYIGGKDYHKKWKDIKYLTAMFINAMRLACNKDTDIDCILRISQLRRGNKENAIM"
     gene            1041..1298
                     /locus_tag="P5293_04930"
     CDS             1041..1298
                     /locus_tag="P5293_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49279.1"
                     /translation="MPLCKKCNKFYPPGFVDRTKEGKECIFCQKEVNEIRYGENNLLI
                     AKREDIILEYQKYLRMIKEENSNIKLLKRGKLEVPEKFKLE"
     gene            1304..1825
                     /locus_tag="P5293_04935"
     CDS             1304..1825
                     /locus_tag="P5293_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRR49280.1"
                     /translation="MRLEAAKVLLLNADYTPLGVISGRKAIKLMSKGKVEIVTATKVV
                     IFNMERTVKFLLPKILRLIKFIRALWKSKVPFNKKHILLRDDYTCAYCGKRSISHMSI
                     DHIIPKAKGGKSIFENVVTSCVPCNQKKDNRTPSQAGMFPRYRAYEPTIMEFIKMQIK
                     RLGLEDALKELGW"
     gene            1854..2075
                     /locus_tag="P5293_04940"
     CDS             1854..2075
                     /locus_tag="P5293_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49281.1"
                     /translation="MIEIKQLREHNEREIKELLGSDGESRIIAPVGGCIKYSYEFHGD
                     HACEWAIIYKDGKEIARRNIAYVGGIIWD"
     gene            2445..3038
                     /locus_tag="P5293_04945"
     CDS             2445..3038
                     /locus_tag="P5293_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRR49282.1"
                     /translation="MDNLYIRQNSLDLNTNQSITVVGCGGIGYWVAKFAAMSGIEKLY
                     LFDPDAFEEHNLNRIDLPIKFIGVNKAQATKAFICSIRPSATVLAFPFILQDHTFPKS
                     DWVVDCTDKFCSQKENEKIAQQYGSRYVKAGYNGEDMSIHNAVADWGEAQDGYTIIPS
                     WVVPAAVVAALTVAKILKYQDKEMASNIKNLLVWGKR"
     gene            3035..3832
                     /locus_tag="P5293_04950"
     CDS             3035..3832
                     /locus_tag="P5293_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HRR49283.1"
                     /translation="MIIKKFIDGKWLITNYNNFLRGKVVSLPVSTPKKEDDEWEIKAE
                     CVTECSKAPKDVIIYFDPIVMAKIDSLMKKYPSREWLGYLIGKSDNPFNIMDLVIPVQ
                     TATSAHVDNVKFPKLEEGMKVLGVIHSHHNMGVHFSGTDDKWINQNHDISVLVSHTDM
                     KIQVRVIVPCGAKKIMEAKPKINYNLNYDEESFLKNAMENIKDHEHQFQPSQSYDDWY
                     NRYCGDSQEDFFDWKKQSIAIDTSDKELSLEEELIKEFSDEPELPIV"
     gene            4168..4626
                     /locus_tag="P5293_04955"
     CDS             4168..4626
                     /locus_tag="P5293_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49284.1"
                     /translation="MRLKKYLNEEFIGVYKYGGWHTEIYENPNANEIRFMPSIQKTEE
                     FLNPEDSIRFIAYAPSEQLFMWASDGGVHEFVLDYLHSKRKIRAAWSLEANGVFAGYA
                     ARSGSKMEFTRGYSIHGDFDWEWLSKYFSNMNRVISYIRANTEPTRTARK"
     gene            4627..5355
                     /locus_tag="P5293_04960"
     CDS             4627..5355
                     /locus_tag="P5293_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49285.1"
                     /translation="MRLKRFIRESILSTREDIFLKFPELLNDLKKMDRSAPVLWRGWS
                     NTSGWIIKVKNDRGTSFFGDFLPTPKKIIEKLNLPSPPVFTTTDHFQSVFFGHPYIFV
                     PIGSYITYYNPEIWDLNSLKRNDNLWKAAEDEVIDKVVSGYKNSHKIPQGIKGEVLLV
                     IDEYWLVSVSSFINETKGKLKTFKNAREIKKYGDVVEVLENFMKFYIWKLKKQVENNP
                     DFIKVIRTWKLPKPWLEQIESVLK"
     gene            5352..5762
                     /locus_tag="P5293_04965"
     CDS             5352..5762
                     /locus_tag="P5293_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49286.1"
                     /translation="MRLKNYLKEKYIGSYTKFSEYFEDERLAIYVNPDARDMQQFKLN
                     GMRFIADPNKRNLYVWDEEIEIHTLMLKYLIRNGFIPITEKYLSGSLLMGSCEVRGSK
                     MVITDIFGSKKLDDSWAWLDKYFSNREDARVFIR"
     gene            5759..6247
                     /locus_tag="P5293_04970"
     CDS             5759..6247
                     /locus_tag="P5293_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49287.1"
                     /translation="MRLKKYLKEEYIGFYKYAGAQAHIYENPTPKEIRKMPDEYTRSQ
                     QFPKNAIKFIAFSPNKKIFIWSSEDEGIHSCVLEYLQDINKIPIDEWDRAPYGLFSGF
                     ASIDGDKMKFEWGLGYNMRDFEHIETEGWEWLSKYFSNMDRVMEYISNFGEYNRWKEE
                     KR"
     gene            6463..7881
                     /locus_tag="P5293_04975"
     CDS             6463..7881
                     /locus_tag="P5293_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRR49288.1"
                     /translation="MNLRRRLLTEAEQLEKWKEYYNSEPILRGAVSILKKITSRGYSA
                     YITGGAVRDIVLGDKPKDIDIATNMPMEELSKIWKVYDIGKSKEFGLVVVREGGFSYE
                     VAQFREDGTYKDGRRPDTVKIVGSFEADAARRDFTINAMAVDAEGNIIDYFDGMKDIK
                     NKVIRTVGNPHDRFGEDYLRIMRTARFASRLGFAIDPKTKQAATELADKVKGLAAERI
                     KEEIFKAASSGGDKFAKYLTYLDEMGILEIILPEIAKLKGMPHVKEFHPEGDVWEHIL
                     SALENSRSKDPLINLSILLHDVGKGVTLDYNERGPIYYQHAEEGVKLVNDIADRLRLS
                     NKERDAILFAVANHMKFQKIIGMKPSKIAKLVGDENWDVLVAVARADEFSRGEKFMAR
                     DDFEKIVDLAVEIKKKWGQKMVNHIMKVVDGHRVMELTGLSPGPQVGKIIKKATEWAI
                     DNNIQDQEEIDKYILKLHRGEK"
     gene            7920..8441
                     /locus_tag="P5293_04980"
     CDS             7920..8441
                     /locus_tag="P5293_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRR49289.1"
                     /translation="MEFPELRQSYSYDCGASVLQSVLFYYGYEIRESDLIKELNVDPN
                     IGAEPADVIRVLQKYGLTPIEKMNMSISELKAYIDQGTPVMMMVQAWPDTPVDWNEEW
                     DWAHWVVAIGYDDKRIYFEDPASPKRTYLTYDELEARWHAYVDQENKSYHYGIAIHGK
                     QKGYKHTNMEHMK"
     gene            8438..8842
                     /locus_tag="P5293_04985"
     CDS             8438..8842
                     /locus_tag="P5293_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR49290.1"
                     /translation="MRLKEYLVEEINVDLGMLIKKWVDRGVRLTAVVKKNRQIPTIEI
                     LDLVISKDERNKGIGSNVIRELIDFADERGYRLELTPAVKDDRMGTTSKNRLQKFYSK
                     FGFVKNIGRHIDYSTSAGMYRDPQKHSIWRRK"
     gene            8839..>9075
                     /locus_tag="P5293_04990"
     CDS             8839..>9075
                     /locus_tag="P5293_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49291.1"
                     /translation="MKFQKYITEAVYREEIEISELIEKLTDFIEKKEKEYSGNYQSLQ
                     YKDYTDEHDRFSREFYSKAIESIRDIKGPLKKLGK"
BASE COUNT         3146 a         1291 c         2030 g         2608 t
ORIGIN      
        1 gaatctctat ctaaatagtc ctcttttttc cgaaagggct aaaatatttg gtaaaataga
       61 agcagctaaa atgttttcat atcaggaata tagatttttc aggcaaaagg tatttgtgga
      121 cttaatattt ttattgtctc tatttccata tccatacttt gcattgagag atcttgagga
      181 aaaaaaccta ccatactgcc gttatatgaa agtatagaaa ggatttccta tgaaacaagg
      241 agaagtcaga aaaattctcc gtgatgataa tgtgagaatt ggattcgaat tagaatgtat
      301 cttcccggcc ttttttcatg ataaaagata taaatctggt ggctatagat atgatagtaa
      361 atacttgttt tctatcgcag tggattattt caaggtaaac agactacctt tcaaagcagt
      421 ttttacagag gaatattatt ataacaataa aaataaatgg gttataaaac ccgataatag
      481 catcggagca gcgggattgg aattggtctc ccctgttatg gaattagagg aatatctcga
      541 tatttgccct gtaatcttct cacatatgaa gaaagtaaaa gctgttacgg ataacaagtg
      601 tgggtttcat ctaaaccttt ccttaaagga taaagggtgg tataatagat tagatgtaat
      661 gaaacttggt ttacttataa atgaacggga gatttacgat ctttttccta tgagaaagaa
      721 caatacatat tgtgaatctg ttcataagga tataaaaaag gttttgaaga aaaaaatagg
      781 tgaatatatt ctcgaaaatt ataaattgaa acctgaattt actgaaagcc atcatatggc
      841 aataaacata tcacatctaa aacaaaaaaa taaatatatc gagttcagat atattggtgg
      901 taaagactac cataagaaat ggaaggatat aaaataccta acagcaatgt ttataaatgc
      961 aatgagattg gcctgtaata aagatacaga cattgattgt attttgagaa tttcacaatt
     1021 aagacggggg aataaagaaa atgccattat gtaaaaaatg taataaattt tatccgcctg
     1081 gatttgttga cagaacaaaa gaaggtaagg aatgtatctt ctgtcaaaaa gaagtaaatg
     1141 aaatcagata tggtgagaat aatttattaa tcgcaaaaag agaggatata atacttgagt
     1201 atcaaaaata tcttcgaatg attaaagagg aaaatagtaa tattaagctt ctaaaaaggg
     1261 gtaaacttga ggtacctgaa aaatttaaac tggagtgata tatatgagat tggaagcagc
     1321 caaagttctt ctgttaaatg ccgattatac tccattggga gtaatcagcg gtagaaaggc
     1381 tataaagttg atgtcaaagg gtaaggtgga aattgttacc gcgacaaagg tggtaatatt
     1441 caatatggag agaactgtga aattcttact tccaaaaatt ctccgtttga ttaagttcat
     1501 cagggcatta tggaagtcaa aagttccctt caataagaaa catattctgc ttcgtgatga
     1561 ttatacctgt gcctactgtg gtaaaaggtc aatatctcat atgtcaattg atcatattat
     1621 tccaaaggca aaaggtggaa agtctatatt tgagaatgtt gtcacttctt gtgttccttg
     1681 caatcaaaag aaagataata gaaccccttc acaggctggt atgtttccaa ggtacagggc
     1741 atacgagcca acgataatgg agttcattaa gatgcaaatt aagcgtcttg gtctggaaga
     1801 tgcattgaaa gaattgggtt ggtaattgat aaaaattaag agggttttaa gagatgatcg
     1861 aaattaagca gttgcgtgaa cataatgaaa gagaaataaa agaattattg ggctcagacg
     1921 gtgaaagcag gattatcgcc cccgtgggag gctgtataaa atattcttat gaatttcatg
     1981 gagatcatgc ttgtgaatgg gctattatat acaaagacgg gaaggaaata gcccgtcgaa
     2041 atattgctta tgtaggagga attatttggg attgagaagt ataggcttga ataccgtagg
     2101 gcatacggga attaacgctt gtggagatgg cttaatagaa ccgtcgttaa aacaagaaaa
     2161 aaaatactta gtaaactaag cagaagccat ggggcttgcc ccatggagtg tcaccaaggg
     2221 atagtgatga ataaaatttc gctttgtaag aacaatccaa tcattttaaa agaaatcggg
     2281 gcggtaggcc accttggcga aacgccggga taagaagcat ccgcctatgg aatgcacgga
     2341 agtcaactcg gaagccatag acttccgtgc tgcttccgag ttgttctctc gcgtctcccg
     2401 gtcggcctct tttatttaca taacaattgt gaaaggaaga tgtaatggac aatctatata
     2461 taaggcaaaa ttctcttgat ctgaatacta atcaatctat aacagttgta ggttgtggtg
     2521 gtattggcta ttgggtcgcc aaatttgcag caatgtccgg tattgaaaaa ctgtatcttt
     2581 tcgatccaga tgcctttgag gaacacaatc tgaaccggat agaccttcca ataaaattca
     2641 ttggagtaaa taaggcacag gccacaaagg cttttatctg ctcaataagg ccttccgcaa
     2701 cagttcttgc attcccattc attttacagg atcatacatt tcctaaatct gattgggttg
     2761 tagattgcac cgataaattt tgttcacaga aagaaaatga aaaaattgct caacagtatg
     2821 gttcccgtta tgtcaaagcc ggatataatg gtgaagatat gagtattcac aatgcagtag
     2881 ctgattgggg ggaggctcaa gatggttata ccattatccc ttcttgggtt gtacccgctg
     2941 ccgtcgtcgc agcccttacc gttgcgaaaa tactgaaata tcaggataaa gaaatggctt
     3001 ccaatataaa aaatctactt gtttggggga aaagatgata atcaaaaaat ttattgatgg
     3061 taagtggttg ataactaatt ataataactt tcttcgtgga aaagttgttt ccttgccagt
     3121 atctacacct aaaaaagaag atgatgagtg ggaaataaag gcggaatgtg tcactgagtg
     3181 cagtaaggca ccgaaggatg ttatcatata ttttgatccg atagtgatgg ccaagattga
     3241 ttctcttatg aagaaatacc cctcaaggga atggcttgga tatcttatcg gcaaatctga
     3301 taatcccttt aatataatgg atttagttat tcctgttcag acagcaacat ctgcccatgt
     3361 tgataatgtt aaattcccga aactggaaga aggaatgaaa gttcttggag ttattcattc
     3421 ccaccataat atgggtgttc atttttctgg tactgatgat aagtggatta atcaaaatca
     3481 tgatatttca gttcttgtca gtcatacaga tatgaaaatt caggtaagag tcattgttcc
     3541 ctgtggagcc aagaaaatta tggaggctaa accgaaaata aattataact tgaattatga
     3601 tgaggaaagc ttcttgaaaa acgcaatgga gaatatcaag gatcatgaac atcagtttca
     3661 accttctcag agttatgatg actggtataa caggtattgc ggggatagtc aggaagattt
     3721 ttttgattgg aagaaacaat caatagctat tgatacttct gacaaggaac tgtctcttga
     3781 agaggaatta atcaaagaat tttctgatga acctgaatta ccaatagttt agaaacagta
     3841 aaaatttcaa ttaaaacggg aataggggat attataaatt gtaatatctc tttttttctg
     3901 taataaaaat ataaataatt ttgatataaa actattagaa tgtaaaagca atatattcct
     3961 cgaccccaca agtaatttgt attttatcat ataatttttt atttgtaaag atgctggatt
     4021 ttttgggtgg agaaaatgat atttttggag gaatatggaa gcgggatatt atggttcagt
     4081 atatgaacac gtaaattccg ccgatgtaag agaggaattg agaagtatga tttatgatga
     4141 agtagaggac gatctggacg aatattgatg aggctgaaaa aatatctgaa tgaagaattt
     4201 ataggtgttt ataagtatgg tggatggcat acggagatat atgagaatcc taatgcaaat
     4261 gaaataagat ttatgccgtc aattcagaaa actgaagaat ttcttaatcc agaagattcg
     4321 ataagattta tagcctatgc accatcagaa cagttattta tgtgggcatc tgatggaggc
     4381 gtccatgaat ttgtattgga ttaccttcac tcaaaaagga aaatccgtgc tgcgtggtca
     4441 cttgaagcca atggagtttt tgcaggatat gcggcacggt ctggcagcaa aatggagttc
     4501 acgagggggt atagtatcca cggagatttt gattgggaat ggctttcaaa atatttttcg
     4561 aatatgaaca gagtaatttc atatattaga gcgaatactg aaccaacaag aacagcaaga
     4621 aaataaatga gattaaaaag atttataagg gaaagtattt tatcgacaag ggaagatata
     4681 tttctaaaat ttccagaatt gttgaatgat ttgaaaaaga tggataggtc tgcccctgtt
     4741 ttatggaggg ggtggtcaaa tacaagtggc tggattatca aagtaaagaa tgacagaggc
     4801 accagttttt ttggtgactt tcttccaaca cccaagaaaa tcattgagaa attaaatctt
     4861 ccatctccac ctgtctttac cactacagat cattttcaat cagtattttt tggtcacccg
     4921 tatatctttg taccaattgg tagctatatt acatattata atcctgaaat atgggatttg
     4981 aactccctga agagaaatga taatctctgg aaagcagctg aggacgaggt aattgacaag
     5041 gttgtttccg gatataaaaa ttctcataag ataccacagg gaataaaagg ggaagttctc
     5101 cttgttatag atgaatattg gcttgtatct gtgtcgagtt ttataaacga gacaaagggt
     5161 aaattaaaga cattcaagaa tgcaagagaa ataaagaagt atggtgatgt ggtagaggta
     5221 ttagagaatt ttatgaaatt ttatatatgg aaattgaaga agcaggttga gaataatcct
     5281 gatttcatta aagtaataag aacttggaaa ttaccgaaac cctggcttga gcaaatcgag
     5341 agtgtattga aatgagatta aaaaattatc ttaaggaaaa atatatcggt tcgtatacga
     5401 aattcagtga atattttgag gacgaaagat tagcgattta tgtaaatcca gatgcacggg
     5461 atatgcaaca atttaaattg aatggaatga gatttattgc tgatcctaat aaaagaaatc
     5521 tttatgtatg ggatgaggaa atcgaaatac acaccttaat gttgaagtat ttgattagga
     5581 acggttttat accaattact gaaaaatatc tttctggttc tcttctgatg ggttcgtgtg
     5641 aagtgagagg atcgaagatg gtaatcacag atatctttgg tagtaagaaa cttgatgaca
     5701 gctgggcgtg gcttgataaa tatttttcca atagagaaga tgcgcgagta ttcatcagat
     5761 gagattgaaa aaatatttga aagaggaata tattggtttt tataagtatg cgggtgctca
     5821 ggctcatatc tacgaaaatc cgacaccgaa agagataaga aaaatgcccg atgagtatac
     5881 aaggtcacag caatttccaa agaatgctat aaaatttata gcattctcac caaataaaaa
     5941 aatattcatc tggtcatcgg aggatgaggg tattcattcc tgcgttcttg aatatcttca
     6001 ggatattaat aaaattccta ttgatgaatg ggaccgggct ccctacggtc tattttccgg
     6061 atttgcatca attgatggtg acaagatgaa atttgaatgg ggtcttggtt acaatatgag
     6121 agactttgaa catattgaaa ctgaaggatg ggaatggctc agcaaatatt ttagtaatat
     6181 ggacagggta atggaatata tttcaaactt tggggagtat aatcggtgga aagaggaaaa
     6241 aagataaagc tcaggaaata tctttcaaaa aaatttttgg ttacatatta cacctgcaga
     6301 gatagagtct gtcaagcagg ctattggtca gggtgatccg attgtgattg gatctttgat
     6361 agtggtggct ttctttgtta tgctggctac ttttggtatg atagacacaa tagtttggct
     6421 agcaataaaa taattatttg gtttaaacgt gacgaagata aaatgaattt gcgtagaaga
     6481 ttattgacag aagccgagca acttgaaaaa tggaaggaat actataattc cgagcccatt
     6541 cttcgtggcg ccgtttctat tctgaagaag ataacatcaa ggggatattc ggcctatatt
     6601 actggcggag ctgtcaggga tattgttctc ggtgacaaac caaaggatat tgacattgct
     6661 acgaatatgc cgatggaaga actttccaaa atctggaaag tatatgatat cggtaaatcg
     6721 aaggagttcg gtcttgttgt ggtcagggaa ggcggtttca gttatgaggt tgcccagttc
     6781 cgtgaggatg gaacatataa ggatgggaga aggcccgaca ccgtgaagat tgtgggttcc
     6841 tttgaggccg atgccgcccg aagggatttc accatcaacg caatggctgt cgatgccgag
     6901 ggaaacatca tcgactattt cgacgggatg aaagacataa aaaataaggt catcaggacg
     6961 gtgggaaatc ctcacgacag atttggtgag gactatttga ggattatgag gacggcaaga
     7021 tttgcctccc gtcttggatt tgccatagac ccaaaaacga aacaggctgc cacggaattg
     7081 gccgacaaag taaaaggtct cgcggccgaa agaataaaag aggaaatatt caaggcagcc
     7141 agttccggtg gggataagtt tgccaagtat cttacctatc tcgatgagat ggggatactg
     7201 gaaatcattc taccggaaat agctaaactg aaaggtatgc cgcacgtcaa ggaatttcat
     7261 ccggagggtg atgtgtggga acatattctt tccgctttgg aaaacagtag aagcaaagac
     7321 ccacttatca atctttcaat ccttcttcac gatgtaggta aaggtgtcac acttgattat
     7381 aacgagaggg ggccaatata ctatcagcac gccgaagagg gtgtcaaact ggtaaatgat
     7441 atagcagaca gattgcgatt gagtaacaag gaaagagatg caatcctctt tgctgtggcg
     7501 aaccatatga aattccagaa aatcattggt atgaaaccaa gtaagattgc taaactggta
     7561 ggagatgaaa actgggatgt gctggttgcg gtagccagag cagatgaatt ttcccgtgga
     7621 gagaaattta tggcaaggga tgactttgag aagattgtcg atcttgctgt ggagataaag
     7681 aagaaatggg gccagaagat ggtcaatcac attatgaagg tcgtggacgg gcacagggta
     7741 atggagttga cgggtctttc ccccggtccc caagtgggta agattatcaa gaaagccaca
     7801 gaatgggcga tagataataa tatccaagac caagaggaaa ttgataaata tatattgaaa
     7861 ctccacaggg gagagaaata gtgagtaaat tttctatttt cataaacaat ataaaattga
     7921 tggaatttcc agaattgaga caatcataca gttatgattg tggtgcttct gtattgcaat
     7981 ccgtcctttt ttattatgga tatgaaatcc gtgaaagcga tcttataaag gaactgaatg
     8041 ttgatccgaa tattggcgct gaaccggcag atgtaatcag ggtattacag aaatacggac
     8101 ttacaccgat agagaagatg aatatgtcca taagcgaact gaaggcatat attgatcagg
     8161 gaacgcctgt aatgatgatg gttcaggctt ggccagatac acctgtggat tggaatgagg
     8221 aatgggattg ggcgcattgg gttgtggcta ttggttatga tgataagaga atttattttg
     8281 aagatccagc ttctccaaaa agaacttatt tgacatacga tgaactcgaa gcaagatggc
     8341 acgcctatgt tgaccaagag aacaagtcct atcattacgg tatagcgata catggtaagc
     8401 agaagggtta taaacataca aatatggagc atatgaaatg agactaaaag agtatcttgt
     8461 tgaggaaatt aatgtagatt tgggtatgct gattaaaaag tgggttgata ggggtgtaag
     8521 attgacagcg gtagttaaga aaaatcgcca aataccaaca attgagattt tagatttagt
     8581 tataagtaag gatgaaagaa acaaggggat tgggagtaat gttattaggg aacttattga
     8641 ttttgcagat gaacgaggat atcgtcttga attgacgcct gctgtaaagg atgatagaat
     8701 gggcacaaca tccaagaata gacttcaaaa attttattca aaatttggct ttgttaaaaa
     8761 tataggtaga catatagact attcaacaag tgcaggaatg tatcgtgacc cacaaaaaca
     8821 cagtatttgg aggagaaaat gaaatttcaa aaatatatta cagaagctgt ttatagagaa
     8881 gagattgaga tttctgaact gattgaaaaa cttacagatt ttatagagaa aaaagagaag
     8941 gaatattctg gaaattatca gagccttcag tataaggatt atacagatga acatgacaga
     9001 ttcagtagag aattttacag caaagctatt gaatctatta gggatattaa aggtccgctt
     9061 aaaaaacttg gtaag
//
LOCUS       DAOTQL010000065         9034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6736_length_9034_cov_4.745851, whole genome shotgun sequence.
ACCESSION   DAOTQL010000065 DAOTQL010000000
VERSION     DAOTQL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6736_length_9034_cov_4.745851"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="P5293_04995"
     CDS             complement(<1..1085)
                     /locus_tag="P5293_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRR49292.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDRMIQYAKLSRNIEKMGDLILDN
                     NDLERERGITILAKNVSVPYKDYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKAIELGKKPVVVINKVDKPNCRPDIVNEQVFDLMFSLNATEEQLDFK
                     TIYGSAKQGWMSTDWKKVTNDITTLFDAIISEIPEPSMLEGSPQMLISSLEYSPYVGR
                     IAIGRVHRGILRQGMQISLCKRDGTFEKSRIKELMVFDGLEKTKTEEVKSGDICAVVG
                     IEGFEIGDTIADYEFPESLPPISIDEPTMSMLFTINDSPFFGKEGKYVTSRHIKERLE
                     KELEKNLALRVKNGLTADSFVVYGRGVL"
     gene            1150..2679
                     /gene="guaA"
                     /locus_tag="P5293_05000"
     CDS             1150..2679
                     /gene="guaA"
                     /locus_tag="P5293_05000"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRR49293.1"
                     /translation="MTDKILILDFGSQVTQLIGRRVREQNVYCEIYPYNHFPPIDESV
                     KGVILSGSPYSVRDSQSPQIDLSAIRGKVPVLAICYGAQYIAQKLGGEVESSHSREYG
                     RAILSINDPLSPLFREIPNNKQVWMSHGDTIKKLPANAVAIASTDNIDNAAFKIIGEE
                     TYCVQFHPEVYHTEIGPAILGNFVKFICGCKGDWTPESFIESTVSEIKQKVGNDHVIL
                     GLSGGVDSTVAAVLMHKAIGDNLHCIFVNNGLLRKNEFSDVLYSYRDMGLNVTGVDTS
                     EKFLYALKGVVDPEEKRKAIGNAFIEVFDEEASKIKNAKWLAQGTIYPDVIESTSVNG
                     PSSTIKSHHNVGGLPKYMNLKIIEPLRSLFKDEVRKVGMKLGVKEDFILRHPFPGPGL
                     GIRVLGEVTKDKLEILREADFYFIDALKKESLYNHVWQAATILLPIKSVGVMGDERTY
                     EYTIVLRAVTSTDGMTADWVHLPYQFLANVSNDIINKVKGVNRVVYDISSKPPATIEW
                     E"
     gene            complement(2690..3604)
                     /gene="xerD"
                     /locus_tag="P5293_05005"
     CDS             complement(2690..3604)
                     /gene="xerD"
                     /locus_tag="P5293_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HRR49294.1"
                     /translation="MKERYQNPYIADFATYLRMELSLSENTISAYCSDITNLNSFMGK
                     DSDGYKIKTLNEVSIEDLDKYIGARMNEGISKRSQARLISSLKSFYGFLEDAGECTNN
                     PTERLDTPKILRNLPVVLSVREVESIINSVDRSTYEGERNKTILEVLYSCGLRVSEAV
                     NLKISNLYLEERFIRVMGKGSKERLVPLGEPAAEALNLYLQIRRRLPINRNCEDVVFL
                     NRRGGKMSRQMIFMIVKNQAKIAGISKEISPHTFRHSFATHLVENGADLRVVQQMLGH
                     ESILTTEIYTHVDSEKWREGILRCHPRR"
     gene            3644..5062
                     /gene="pyk"
                     /locus_tag="P5293_05010"
     CDS             3644..5062
                     /gene="pyk"
                     /locus_tag="P5293_05010"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRR49295.1"
                     /translation="MYKKKTKIICTISDKRCDAGFIRSLYEAGMNVVRINSAHTTLES
                     SMEIVNNVRKVSDRIAILIDTKGPEIRITEMDNLDGFKVEKGDTITFADGVDLVSSKE
                     LLYTNYSNLVSEVPIGVKILIDDGEISLTTVGKSIDKLICKADNNGIIKGKKSINIPN
                     VKIMLPSVSEKDKNFIIWATDNNLDFVAHSFVRNKSDLMAVQKIIDDKKSHLKIIAKI
                     ENQEGVNNIDEILSHCYGIMVARGDLGVEIEYQKIPIIQNKIIDKCHRRKKPVIIATQ
                     MLHTMIEHPRPTRAEVTDVANAVLQSADAIMLSGETAGGAYPLEAVQTMTKIALEIEQ
                     HQTPFYNIELQNVTAPIAAVLAKCMVDATSKLPIKAIVLDTLSGRTGRYISAFRPNVP
                     VYAKCYQDYVMRELALSYGINAFCSDPYDSKEDFIKTEVKALLKDKCFNRNDTIGIIA
                     GSFGTSIGATYMEISTALNMIM"
     gene            complement(5009..5674)
                     /gene="nth"
                     /locus_tag="P5293_05015"
     CDS             complement(5009..5674)
                     /gene="nth"
                     /locus_tag="P5293_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRR49296.1"
                     /translation="MDLAERYKSVIEWFEKNMPLAVSELSYESPFQLLVAVILSAQCT
                     DKRVNMVTKELFKKYSSPESLASATPEEVFRYISSVTYPNSKSKHLVNMSKMIVERFD
                     SKVPSDPLLLQELPGVGRKTANVVASIVYGKPVIAVDTHVFRISRRLGLSKSKTPYGV
                     EQDLSANIPEDKRAIAHHWLILHGRYICVARNPKCSVCGLREWCLHYHVQGSTDLHIS
                     STD"
     gene            complement(5677..6402)
                     /locus_tag="P5293_05020"
     CDS             complement(5677..6402)
                     /locus_tag="P5293_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR49297.1"
                     /translation="MPLDNNDYLINLSGVDIIKEGNLILTDVNFSVKAGDFVFIIGRV
                     GSGKSSVIKTIIGEIPVSRGEAAVAGFDLKTIRRKQIPYLRRQIGVVFQDFQLLVDRS
                     VYDNLKFVLQSTGWKNDKEIDRKIKSKLESVGMLLKAHKMPHQLSGGEQQRVAIARAL
                     LNEPQIILADEPTGNLDTETAYEIMDLLMTIHKEQEPAIVMVTHNRSLLKKYPAKMML
                     CEDLYFKEIEESQEIDFSQLFYE"
     gene            6474..>9034
                     /locus_tag="P5293_05025"
     CDS             6474..>9034
                     /locus_tag="P5293_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR49298.1"
                     /translation="MKRAIIRYGYSTTLIVILNTLLTMSAQESQLLSKYAAHYEKGIE
                     LYSKMLYQAADSEFDMALESLQSNSPIQRAEVEGYKVLIAIKLDRPNKEALVKDYVSN
                     YPFSNQLSSIYLSFATHFFEHENYNRSLELLNQVNTRNLNTLQRAEYNFKLGYSNMRV
                     GNLKKALSIFDSMSSGKYTSFTNPALYYSAYIHYMLRDFKKAKEGFSKIKGDPQFDIL
                     ARYYILESDFMLKDYDAVITNGEKLYENLAEEFKAKCARILSEAFYNKGETEKAKIYF
                     DRYSGSNSRLSRNDHYYEGMISYKLNNYPAAIESFSKVISIADTLSQNAKYHLGYCYA
                     EIKNKQMALQLFKEASGMNFDKLIKEDAMFNYAKLSFDLNSDITKFQSYLAEYSPPDT
                     KFNEIQNYIAGHYLLSGEYKAAVDALKLISHPSDRDKANLQKASFLRGMQLMEKGGYR
                     EAIQNFELSRSCYTGNIYIKNLASFWLAEAYFRNGQIQKSIEINLALLNKELSFKKTS
                     EYPVAVFNLAYGYFKLPDYKKAEENFKRYLANSSSVPDYISEAKLRTGDCLFMQKNYS
                     DAIEFYSQVDPRDQLFTTYARYQMAIAYGLLGNDNKKKEILKEVADSKNKNRYYPEIL
                     YELGRTLVQRGEGDEAVKYFKELSEEYKDTPYFTKALLELGLISLNSKDYSEAIQYYK
                     TILEKAPDSPDAQSAIAGLENIYQERGEAEEFLSYLDQKGLSTTKSASDKELMLFNSA
                     EKLYLSGNVAGAISSLNSFISKYPSSVKLSQAYYYLGELYLKSDKPEHALDAFLTVMQ
                     KGEGSFVELATLNYARIAYRLEDYKRALEGYSTLSIIAKLPNNKIEAEVGRINSF"
BASE COUNT         2983 a         1819 c         1543 g         2689 t
ORIGIN      
        1 agcactcctc ttccatagac aacaaatgag tctgcagtca gaccattttt aactctcagc
       61 gcaagattct tctcaagttc tttttcaagt ctctccttta tgtgtcttga tgtaacatat
      121 tttccctctt tcccaaaaaa aggagaatca tttattgtaa acaacatgct cattgtaggc
      181 tcgtcaatcg aaataggggg caaagattct ggaaattcat aatctgcaat agtatctcct
      241 atttcaaaac cttctatacc aacaacagca catatgtcac cacttttcac ctcttctgtc
      301 ttggtttttt caagaccatc aaacaccata agttctttaa tacgactctt ttcaaatgtt
      361 ccatctctct tacaaagaga tatttgcatc ccctgtctga gaatacctct atgaaccctt
      421 ccaatagcaa ttctgccaac ataaggagag tactccaaag aagaaattag catttgaggt
      481 gacccttcaa gcatcgatgg ttcaggtatc tctgaaatta tggcatcaaa aagagtagta
      541 atatcattag tcactttttt ccagtcagtt gacatccacc cttgtttggc acttccataa
      601 attgttttga aatcaagttg ctcttctgtg gcattaagac tgaacattaa atcaaatacc
      661 tgctcattca ctatatctgg cctgcaatta ggtttgtcaa ccttattaat tactacaaca
      721 ggtttcttac ccaattctat tgctttttgg agcacaaatc ttgtctgcgg cattgtccct
      781 tcaaaagcat caaccagaag caggacacca tcagccatat taagaactct ttcaacttct
      841 ccaccgaaat ccgcatgtcc cggagtatca ataatattta tcttatagtc cttataagga
      901 acagagacgt tttttgcgag tatagtaata cctctctccc tctcaagatc attattgtcg
      961 agaataagat cccccatctt ttcaatattc cttgagagct tggcatactg tatcatcctg
     1021 tctaccaaag tagttttgcc atgatcaaca tgagcaataa tggcaatatt tcttattttt
     1081 tgcataattc aggccgcaaa agtaatattt tatttacttt tgcgggtcat tatttacaaa
     1141 aataatctca tgacagataa aatattgatc ctcgactttg gctcccaagt aactcaactc
     1201 attggcagaa gagtgagaga gcaaaacgtc tattgtgaaa tttatccata caaccatttt
     1261 ccccctattg atgagagtgt aaaaggagtc attctttctg gcagtccata ttcagtcaga
     1321 gactctcaat cacctcaaat tgatctgtct gccatcagag gtaaagtacc tgttctggct
     1381 atttgttatg gtgctcaata tattgcacaa aaattaggtg gagaagttga gagttctcat
     1441 tcaagagaat atggaagagc tattttatct attaatgatc ccctgtctcc actattcaga
     1501 gaaataccaa ataacaaaca ggtatggatg tcccatggcg atactataaa aaaactacct
     1561 gcaaatgccg ttgcaattgc ctcaacagac aatattgaca atgctgcttt taaaattatc
     1621 ggagaagaaa catactgtgt acagtttcat cctgaagttt atcacacaga gattggccca
     1681 gctattcttg ggaattttgt aaaattcatt tgcggatgta agggagactg gactccggaa
     1741 tcttttattg aaagcactgt ttcagagatt aaacagaaag tcggcaacga tcatgtcatt
     1801 ctcggacttt ctggaggagt agattcaact gttgctgcag tactgatgca taaagcaatt
     1861 ggtgataacc ttcattgtat ttttgttaat aacggattat taagaaaaaa cgaattttcc
     1921 gacgtcctct attcatacag agatatgggc cttaatgtaa caggcgtcga tacttctgaa
     1981 aaatttcttt atgcactcaa aggtgttgtc gaccctgagg aaaagcgcaa ggccatcggt
     2041 aatgccttta ttgaagtatt tgatgaagaa gcaagcaaga ttaaaaatgc aaaatggctg
     2101 gctcaaggga cgatttatcc tgatgttatt gaatctactt cggtcaatgg tccaagttca
     2161 accatcaaat ctcatcacaa tgttggagga ttgccaaaat acatgaatct caaaatcatt
     2221 gaaccccttc gctctctatt taaggatgaa gtacgtaagg ttgggatgaa attgggagta
     2281 aaagaagatt ttattttacg acatcctttc cccggtccgg gacttggcat aagagtattg
     2341 ggagaggtta ctaaagacaa attggaaatt ctaagagaag cagattttta tttcatcgat
     2401 gctcttaaaa aagagagtct ttacaaccat gtctggcaag cagccacaat ccttttgcca
     2461 atcaaaagcg taggagtaat gggtgacgaa agaacatatg aatacactat cgtactccga
     2521 gccgtcactt cgacagatgg aatgacagca gattgggttc atcttccata ccaatttctt
     2581 gcaaacgttt caaatgacat tatcaataag gttaaaggtg tcaatagagt ggtttatgat
     2641 atttcttcca aaccacccgc aacaattgaa tgggaataat aacattaatc taacggcgtg
     2701 gatgacacct tagaattcct tctctccact tctcactgtc cacatgagta tatatttcag
     2761 tagttaatat actttcatgc ccgagcattt gttgaactac cctcagatct gcaccgtttt
     2821 caaccagatg agtagcaaaa gagtgccgaa aagtatgagg cgaaatttcc tttgaaatac
     2881 ctgctatctt tgcctggttt tttactatca taaaaatcat ctgacgagac atcttacctc
     2941 cccgtctgtt taaaaacact acatcctcgc aattgcgatt tattggcaat cttcttctta
     3001 tttgaagata taaattaaga gcctcagcag caggttctcc cagcggaacc aatctttcct
     3061 tgctcccctt ccccattact cttataaacc gttcttcaag atagagattg gatattttga
     3121 gattgacagc ttcgctaacc cttagtccac aagaatataa cacttcgaga atggttttat
     3181 tcctctcacc ttcataagtg ctccggtcaa ctgagttaat aattgattct acctccctga
     3241 cagacagaac tacgggaaga ttacgtaata ttttaggtgt atccaacctt tccgtcggat
     3301 tgttagtaca ttctccagca tcttccagga aaccataaaa agattttaac gagcttatca
     3361 atcgagcctg agaacgcttt gagatacctt cattcatcct tgcgccgata tatttatcaa
     3421 gatcctctat cgatacctca ttcaatgtct tgattttata tccatcacta tcctttccca
     3481 taaaagaatt cagattcgta atatcagaac agtacgcact aatggtattt tctgaaagtg
     3541 acaactccat tctcaaatag gtggcaaaat ctgcaatata agggttctga tatctctcct
     3601 tcaaaattct gtatacattt gtataacaaa attagaacaa attatgtata aaaagaaaac
     3661 taaaataatc tgcacaatat cagataaaag atgtgatgcc ggttttattc gttctctcta
     3721 tgaggcaggc atgaatgtcg taagaataaa ctccgcacat accactcttg agtcttctat
     3781 ggaaattgtc aataatgtaa gaaaagtttc tgacaggata gcaattctca tagacactaa
     3841 gggaccagaa atcagaatca cagagatgga taacctcgat ggctttaaag ttgaaaaggg
     3901 tgataccata acctttgcag atggagtcga ccttgtctca tcaaaagaac ttctttatac
     3961 caactactcc aatcttgtaa gtgaagttcc cattggtgta aagatactga tcgacgatgg
     4021 agaaataagt ttgaccaccg taggtaaaag catcgataaa ttaatatgca aagcagataa
     4081 taatggaatc ataaaaggca aaaagagtat taatatacct aatgtcaaga taatgctccc
     4141 atcagtctct gaaaaggata aaaattttat aatctgggct accgacaata atctcgattt
     4201 cgtagcacac tcatttgtta gaaataaaag tgaccttatg gctgtccaaa aaataatcga
     4261 tgacaaaaaa agtcatctta aaattattgc aaaaatcgag aatcaggagg gtgtcaacaa
     4321 tatagatgag attctttccc attgctatgg gataatggtt gcacgtggag atttgggagt
     4381 tgagattgaa tatcagaaga ttcctatcat ccaaaataaa ataattgata agtgtcaccg
     4441 aagaaaaaaa ccggtaataa ttgccacaca gatgcttcat actatgattg aacacccacg
     4501 accaacaaga gccgaagtaa ctgacgttgc aaatgcggtt cttcaaagcg ctgatgcaat
     4561 aatgctcagt ggagaaactg cagggggtgc ttatccgctt gaggctgttc agacaatgac
     4621 taaaattgca ctcgaaattg aacaacatca aactcctttc tataatatag aattacagaa
     4681 cgttactgcc ccaattgcag cagttttggc aaaatgtatg gtagatgcaa catcaaaact
     4741 tcctatcaag gcaatagttc tagatacatt atcaggaaga acaggaaggt atatttcggc
     4801 gttcagacca aatgttccgg tttacgcaaa atgctatcaa gactatgtga tgagagaact
     4861 tgctttatca tacggaataa atgctttttg ctcagaccca tacgatagca aagaagactt
     4921 tattaaaacc gaagtgaaag ctcttctcaa agataaatgc ttcaacagaa atgatactat
     4981 tggaattatt gccggaagct ttggaacatc aatcggtgct acttatatgg agatcagtac
     5041 tgccctgaac atgataatgt aggcaccatt ctctcaaacc gcaaacagag catttaggat
     5101 ttcttgctac gcaaatatac cttccatgca aaattagcca gtgatgagct atcgcccttt
     5161 tatcttcagg aatattggct gaaagatcct gttcaactcc ataaggtgtt ttgctttttg
     5221 agagaccaag ccgcctcgaa attctaaaaa cgtgagtgtc gactgcaatt acgggtttcc
     5281 cataaactat tgaagccacc acattggctg tttttcttcc cacacccggt aattcctgta
     5341 aaagtagtgg atctgatggt actttggaat caaacctctc aacaatcatt ttggacatgt
     5401 tcacaagatg cttgcttttg ctgtttggat aagtaactga agaaatatat ctaaaaacct
     5461 cttctggtgt agccgaagca agtgattcag gcgaagaata ctttttaaac agctctttag
     5521 ttaccatatt tacccttttg tcagtacact gagctgataa tataactgct acaagcagtt
     5581 ggaaaggtga ttcataggaa agttcactga ccgccagcgg catgtttttt tcaaaccatt
     5641 caataactga cttataacgt tcagcaagat ccatttctat tcgtaaaaaa gctgtgaaaa
     5701 atctatctcc tgactctctt ctatctcttt aaaatagaga tcctcacaca gcatcatctt
     5761 agcaggatat ttcttcaata aagatctatt gtgtgtcacc atgacaattg caggctcctg
     5821 ttccttatgt atagtcataa gcaggtccat gatttcataa gctgtttcag tatcaagatt
     5881 gcctgtaggt tcatcagcaa gaataatctg aggttcatta agaagtgccc ttgctatagc
     5941 tactcgctgc tgttcccctc cagaaagctg atgaggcatt ttatgagcct taagcaacat
     6001 gccgacactt tctaatttcg atttaatttt tctgtcaatc tccttatcat ttttccaccc
     6061 tgtagactga agcacaaatt tcagattatc atagacactt ctgtcgacaa gaagttggaa
     6121 atcctgaaat accacaccta tttgacgtct gaggtaaggt atctgcttcc ttctgattgt
     6181 tttaagatca aaacctgcta cagcagcttc tccccttgat actggaattt ctccaataat
     6241 agttttgatt accgaagact ttccactacc aactcttcca attataaaaa caaaatcacc
     6301 tgcttttaca gaaaaattta catctgtcaa aatcagattt ccctccttga taatatcaac
     6361 tccactaaga tttatcaagt aatcattatt gtccaacggc attatattcg ctatttcatt
     6421 acaaaattac taattttggg agctaaacaa caatagaata taaaatttat taaatgaagc
     6481 gagcaataat aagatatgga tatagcacta cccttattgt tattttaaat acactcctca
     6541 caatgtcggc tcaggagtca cagttattat ccaaatatgc cgcacattat gaaaaaggaa
     6601 ttgagcttta cagcaaaatg ctttatcagg ctgcagactc agaatttgat atggctcttg
     6661 aatctcttca atcaaattca ccgattcaaa gggcagaagt tgaaggttac aaagtgctca
     6721 ttgccataaa actcgacaga ccaaacaagg aggctctggt aaaagattat gtgtcaaact
     6781 atccattttc caatcaactt agttcaatat atttgagttt tgcaacccat ttttttgaac
     6841 atgaaaacta taatcgttct ctggaactcc ttaaccaggt taatacacgt aatctcaaca
     6901 ctttgcaaag agcagaatat aattttaaac ttggatattc aaacatgaga gtcgggaacc
     6961 ttaaaaaagc actttctatt ttcgattcca tgagttccgg taaatacact tcatttacaa
     7021 atcccgcatt atattattct gcatatattc actatatgct cagagatttc aaaaaagcta
     7081 aagagggatt ttccaaaatc aaaggagatc cgcaatttga cattcttgca agatattaca
     7141 ttctcgaatc tgattttatg ctcaaagatt atgacgctgt cattaccaat ggtgaaaaac
     7201 tatacgaaaa cttggcagaa gagttcaagg caaaatgtgc aagaatactt tcagaagcat
     7261 tttacaacaa aggagagaca gaaaaagcca agatctattt tgatagatat tctggcagca
     7321 acagcagact ttcgagaaat gatcattatt atgaaggaat gatttcatac aaactaaaca
     7381 attatcctgc agctattgaa tccttcagta aagtcatctc cattgcagat actctttctc
     7441 aaaatgccaa ataccacttg ggctactgct atgctgaaat aaaaaacaag cagatggcgc
     7501 ttcaattatt taaggaagct tccggaatga actttgataa acttatcaag gaagatgcca
     7561 tgtttaacta tgcaaaactg tcatttgacc ttaactctga tatcacaaaa ttccaaagtt
     7621 atcttgccga atattcacca cctgacacaa aatttaatga aattcaaaac tatatagcag
     7681 gtcactactt gctttcggga gaatacaaag cagccgtgga cgcgctcaaa cttatcagtc
     7741 atccctctga cagagataag gccaaccttc aaaaagcctc atttctaagg ggtatgcaac
     7801 tcatggaaaa gggcggttac cgagaggcga ttcagaactt tgaactatca agaagctgtt
     7861 ataccggcaa catatatatt aaaaatcttg cctctttctg gcttgcagaa gcttatttcc
     7921 gaaatggaca aattcaaaag tctattgaaa ttaatttagc attgctaaac aaggaattaa
     7981 gtttcaaaaa aacatctgaa tatcccgttg ctgtttttaa cctggcatac ggatatttta
     8041 agcttcctga ttacaagaaa gctgaagaaa attttaaaag atatcttgca aactcatcat
     8101 ctgtacccga ttacatttca gaagcaaaac ttcgcactgg agattgtttg tttatgcaaa
     8161 aaaattacag tgatgctatt gagttctatt cacaagttga cccaagagac caacttttta
     8221 ctacctacgc aagatatcaa atggcaattg catatggtct tcttggcaac gataacaaaa
     8281 agaaagagat actcaaagaa gtagcggatt ccaaaaacaa aaatcgttac tacccagaga
     8341 tactgtatga acttggaagg acactcgttc aaagaggaga aggagatgaa gctgtcaaat
     8401 atttcaaaga attaagtgag gaatataagg ataccccata ctttaccaaa gcgctccttg
     8461 aacttggtct catatctctg aattctaaag actattctga agcaattcaa tattacaaaa
     8521 caatattgga aaaagctcca gattctccag atgcccaaag cgctattgcc gggcttgaga
     8581 acatatatca agaaagggga gaggcagaag aatttctctc atatctcgac caaaaaggtc
     8641 tatctacaac aaagtcagca agcgacaaag aattgatgct gtttaattct gccgaaaagt
     8701 tatatctctc cggcaatgtg gcaggagcaa ttagttcact taactcgttc atttcaaagt
     8761 atccttccag cgtcaaactt tcacaagcct attactatct tggagaactc tatcttaaat
     8821 ctgataaacc tgaacatgct cttgatgcat tcttgacagt aatgcaaaaa ggagaaggtt
     8881 cttttgtgga gctagcaaca cttaactatg caaggatagc ctatcgactc gaagactaca
     8941 aaagagcact ggaaggttat tccacactta gtatcattgc taaacttcct aataataaaa
     9001 tagaagcaga agtgggtaga attaattctt tttt
//
LOCUS       DAOTQL010000066         9004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6764_length_9004_cov_6.494804, whole genome shotgun sequence.
ACCESSION   DAOTQL010000066 DAOTQL010000000
VERSION     DAOTQL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6764_length_9004_cov_6.494804"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="P5293_05030"
     CDS             complement(<1..900)
                     /locus_tag="P5293_05030"
                     /inference="COORDINATES: protein motif:HMM:NF018736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49299.1"
                     /translation="MREESKKLIEKWKAVLTEGNKIRSQKILKSTAIMLENEYKYLSE
                     AGNWSEVGRGSPYAISGDFHQIAVPMVRRTFPELIAHDIVGVQPLTGPVGLAFALRFR
                     TGGPSTYGGSSDVELGYNTMDITYSGSYTTSAGEVLGSNVTGDVGLGIGSGTAINEVN
                     LTVEKAQVEAKTRKLRSRWSLEVAQDLKAMHGLDLEEEMMDILAYEITAEIDREIVNV
                     VNSIAESSTWSYSTSADGRWESEKYRNLYNVVVRRANKIAIDTRRGAGNFVLASPYTC
                     AAFEGLSSFVIHPTDGEINTLVAG"
     gene            complement(924..1832)
                     /locus_tag="P5293_05035"
     CDS             complement(924..1832)
                     /locus_tag="P5293_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49300.1"
                     /translation="MEEILKVLGIEKLDESQITSIKEKLDAIVDVKSRERADSLLKEE
                     SEKLIEEYEGKFEEYKNDITSKFSNFVDQVLEEELKLPEKIVDYAKKGELYDDVIQEL
                     KVRLAIDEGVLNEEIRELLREAKEEIIRLKDELNKETAGRLQAEEDARALASHLYLRK
                     KCDGLTESQRRKVIDLLGDLNEKTEIDRKFQIVVDTVLEQDAPDAEFGLKPGTQDPDA
                     EAKTNDCICPSCGAHATTKGACASMACPSCGASMKDASDPTPAKDMGQGKIEVEDQEG
                     KKVEEADDPFSKYLKGAVAILKENRF"
     gene            complement(1877..2329)
                     /locus_tag="P5293_05040"
     CDS             complement(1877..2329)
                     /locus_tag="P5293_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49301.1"
                     /translation="MSILDRIDEFLTREQKRLRKRDPKRCLKENFDMDDLLDKMGNFI
                     YNLDPNVLDMEGKRIREEILAAFEPPFELTDREWNFDDTHLSDDTSELDTMEDDEVVD
                     NTIADEDAELEDKVYEENEVKKKRMTIDGITTTTHIKERKKRNLQKKK"
     gene            complement(2452..2865)
                     /locus_tag="P5293_05045"
     CDS             complement(2452..2865)
                     /locus_tag="P5293_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49302.1"
                     /translation="MDKNDLKEEKIDDLLQKKSPELLKAKKKAKNRAYDVMNKYIGAG
                     PTYTGMISFPESKQLTEADIPRAIVALMNILSFVRKTNKEFETNLNSLKAMWRDGLFE
                     MDEYENIADAYKHIIKGLTIFENEIEGKIKTLRGK"
     gene            complement(2865..3503)
                     /locus_tag="P5293_05050"
     CDS             complement(2865..3503)
                     /locus_tag="P5293_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49303.1"
                     /translation="MKLITELSHDIKFIVEESEKKEKSLYITGIYSTADQENNNKRIY
                     SRHILEREISKLEERLVNRSLWGELGHPPNPEINPDKIAILTEKLEWDGPNVIGRSKV
                     LDTPMGNIAKTLIKEGRMGISSRGLGSVNENGYVNEDFYLITWDMVTDPSNHPSWVNG
                     IYEGQDFEPPQKRVVEEPESVIEEPKISLKEAQEAYYRKIWQVISDIEKRIK"
     gene            complement(3575..3922)
                     /locus_tag="P5293_05055"
     CDS             complement(3575..3922)
                     /locus_tag="P5293_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49304.1"
                     /translation="MKKIEEKIDMYLKEAVFDEPKPQEFSAGDQKLYPPVGGSVPGTY
                     DPTRDAGSITQGLKRLKYYLTMANTPEKFAKAMSLAHELVVKYPNNQRIAQVINVIKT
                     TPIRILKTVGGEE"
     gene            complement(3919..4119)
                     /locus_tag="P5293_05060"
     CDS             complement(3919..4119)
                     /locus_tag="P5293_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49305.1"
                     /translation="MDEKDIKKALDSFENDDFISAKEIVKNVVKQAKNEYLKSKLGLT
                     KDIEPPKQKQVVQKLAEEKKKK"
     gene            complement(4131..4391)
                     /locus_tag="P5293_05065"
     CDS             complement(4131..4391)
                     /locus_tag="P5293_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49306.1"
                     /translation="MKIDRIMNKYLSLVEADYSDTIQLLQKQIRDSRKLMRKAIEDTD
                     DSDKKEQIRKRGTAHIESLQKRIESYQERAKAEREREKAAKK"
     gene            complement(4381..6816)
                     /locus_tag="P5293_05070"
     CDS             complement(4381..6816)
                     /locus_tag="P5293_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49307.1"
                     /translation="MKLRNFFHQVNEEFLDQIFEAKKLVPKQKSVIKPEPKSEERPKN
                     KIKPEQIKKWNSWSPGEQNYVIQRSPEGVYAQAVKDNIIKLKDKASKPIAHEKDEEAK
                     GTTEPIKKDDSIKIPAFDKIEASNIPDDRKSSRLHDVVDAMKPEAAKEFLNSIVKKYG
                     GIVPSWVADVHNHWKNIIEKKPTKEKEKKPEKPEKKPTKEKEVPTSDMKSNVSYISDK
                     IKTNKLLKMHVKGKEIKQIYDDLLQQLINLDKAKPSEKKAKAEKIKNDFGLRIGATGV
                     LNVDLVGELHHDSKKLIGSGLVARNIAKYFEDIGVFLKAEVGEEGKTKTLSTSSKPNL
                     GMTGLDAKDNKYVAEIFSEGPLAALEPGFKKLYMPIDDKGNPIDNKGGKNSLEYLKHS
                     VSNNKALDRTIEASEKLVKAGKLKKEFVAELKAHKTRMLEIVDNEKSIPSADAEKAVG
                     DSYATLACKLSEIDKDAAESVMKNFAEMALYDTEIARGKEVYLPSAGNFPSGDKLKIT
                     RKGTKIEKIQSVSIKYGKKGDFSSYGFPGEASKYCLYHKDPKKRELLKSRPGENGYTI
                     GVKDSIIDDRKEFDAILKNSGGIDKAIKDMDKFYKLVKEYKEATAETIAKKGVLAASE
                     SAREKLDRIYTKRMRELVDYDKLSDVIGYDNARMVLGMPARKGDPDTLSGPNCFMSAI
                     TFSHVLQTSNGLEMLEHNHQEYINGEYHSETNTYEDGTINLKLWKLGWRPYGSRVQGL
                     NAGFNKRRIYLTTPEQRAAARAEKKAAEKEAKKKQRLQKASYKYPWNRNTEILVEKYI
                     NNYKYIRGVSNED"
     gene            complement(6821..8497)
                     /locus_tag="P5293_05075"
     CDS             complement(6821..8497)
                     /locus_tag="P5293_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HRR49308.1"
                     /translation="MAWYDFLRGKKDEDVRLDESMKVFVGKGDIPMKSEVEATKGEGV
                     EDYMLISGYGSLGYGNLNTFYNRYINKVFETEVAKIIEYRKMAEYPEIGDVIEDAVNE
                     STLTDNNNRVFSLIITDNKLSENKNVIKNLYKEFDDLFYRRIEIDRIIDDLMRTYYID
                     GRVYYERIINTKAPSQGIQAIKKLPSETMDYVYDPMTGQILYYYQYLAPNTKRPINRE
                     EAEKDPKVVVFNPEQIGYINYGIYGRTKGEVYGYLEKARVPYNQLKLLETSVIIYRII
                     RAPERFVFKIDTGNMPKDKAMKFVEKIKTKFIKRQTYDPQTGQLSQEPEVLSILENFF
                     LPQSADGRGSSIETVGGNPAGFSELDDIYYFARKLYRALKYPASRVTAGQEKREAEIV
                     VGGSHTGEISRDEVKWAKFLEKHQKRFCDEFKDLFLMHLAFRGLKKQYGIEKDMLTIN
                     MVPPSHYKESLEQGFVEVRHSNYNALAGNQEFSKYFLMKKYLGWTDEEIEENIRCLKD
                     KDKLFQPPPEPGMEGEMGSEELGGEKEFGAGETGAGSPAPEEGVPVEEIK"
     gene            complement(8499..8891)
                     /locus_tag="P5293_05080"
     CDS             complement(8499..8891)
                     /locus_tag="P5293_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49309.1"
                     /translation="MKGTCEPIDPVSGKVICGLYELFGDIRKCPNFVENIFTPNPLDS
                     DQGVKIVSDCIPKRTLLMIQELHNRTVALQQAQEQLRNETIWTEVVAHVIGKNIGIDL
                     TKFVEERQRQLRVIELKKQEEEKAQIEE"
BASE COUNT         2237 a         2062 c         1412 g         3293 t
ORIGIN      
        1 acccgctaca agggtgttta tctcaccgtc tgttgggtga attacgaagc ttgaaagtcc
       61 ttcgaatgcg gcacaagtgt aaggtgatgc cagaacaaag ttaccggcgc ctctccttgt
      121 gtcaatcgcg atcttgttgg ctctacgaac aaccacattg taaaggtttc tgtatttctc
      181 agattcccat ctaccatcag cggatgttga gtatgaccaa gtagaggatt ccgcaatact
      241 attcacaaca ttgacgatct ctctgtcaat ttccgccgtg atttcgtatg cgagaatatc
      301 catcatctct tcttcaagat caagaccgtg cattgccttc aggtcttgtg ccacttcaag
      361 tgaccatctg cttctcaatt ttctcgtttt agcctcgact tgcgcctttt caactgtaag
      421 gtttacttcg ttgatagccg taccagaacc aatgcccaga ccaacatcgc ccgttacatt
      481 ggagccgaga acttcaccag cagaggttgt atatgatcca gaataagtga tatccatcgt
      541 gttatatcca agttcaacat ctgacgaacc gccataagta gatgggcctc ctgttctgaa
      601 tctgagcgcg aacgctagcc ctactgggcc tgtgagtggc tgaacaccaa cgatatcgtg
      661 ggcgatcagc tctggaaagg ttctccttac catcggaact gcaatctgat ggaaatcacc
      721 ggaaatcgca taaggcgatc cacggccgac ttctgaccag tttccagctt cactgaggta
      781 cttatattca ttctccagca taatagctgt agatttgaga atcttttgag aacgaatttt
      841 atttccctct gtaagaacag ctttccactt ctcgatcaac ttcttactct cttctctcat
      901 ttcagaatct tcctcctata aatttagaat ctattctctt tcagaattgc gacagctccc
      961 ttcaaatatt tgctgaaagg atcgtctgct tcctcgactt tctttccctc ttgatcttca
     1021 acttcgattt tgccttgacc catatccttt gcaggtgttg gatctgaagc atctttcatt
     1081 gaagcaccac aagacgggca agccatcgag gcacatgcgc cctttgtggt cgcgtgagca
     1141 ccacaggatg gacaaataca atcatttgtt tttgcctctg catcaggatc ctgtgtgccg
     1201 ggttttaaac caaattcggc atcaggtgca tcctgttcga gaacggtatc aacaacaatc
     1261 tgaaacttcc tgtcaatttc tgtcttttca ttcaggtcgc ccagaaggtc aataaccttt
     1321 ctcctttgtg actccgtgag tccgtcacat ttctttctca aataaaggtg agatgcgaga
     1381 gcacgggcat cttcctctgc ctgtagtctc cccgctgttt ccttgttaag ctcatccttc
     1441 aatcgaatga tctcttcttt ggcttcacgg agaagctctc taatttcttc attcagaaca
     1501 ccctcatcaa tggccaatct gactttgagt tcctgaatga catcatcata aagctcgcct
     1561 ttctttgcat agtccacaat tttctcaggc agtttgagtt cttcttcaag aacttgatca
     1621 acaaagttcg aaaactttga tgtgatatca ttcttgtatt cctcaaattt gccctcatat
     1681 tcctcgataa gtttttctga ctcttccttc aaaaggctgt ctgctctctc acgggatttt
     1741 acatccacga ttgcatccag tttctcttta atagaagtaa tctgtgactc atcgagtttt
     1801 tcaataccga gcactttcaa gatttcttcc atctattagt tacctcctat agtatttcta
     1861 ttctttttca agaatattac ttctttttct gtaaatttct cttttttctt tccttgatat
     1921 gagtcgttgt agtaatacca tctattgtca ttcttttctt ttttacctcg ttttcctcat
     1981 atactttatc ttcgagttca gcatcttcat ccgctatagt attatcaacg acttcatcat
     2041 cctccattgt atccaattcg gatgtatcat cagaaagatg tgtatcgtca aaattccatt
     2101 ctctatctgt caactcaaat ggaggctcaa aggctgcaag aatttcttcc cttattcttt
     2161 tgccttccat atcaagaaca tttggatcaa ggttatagat gaaatttccc attttatcga
     2221 gcaaatcatc catatcaaag ttttctttca gacatctttt gggatctctc ttacgaagcc
     2281 tcttttgctc ccttgtaaga aactcatcaa ttctatcaag aatagacata ttctcctcca
     2341 ttttaaatta tttatcggtt tttcggttat ttacaacaga ttggttaaaa cacacgcgtg
     2401 gcaatgattt tggactacac aaattacttt acaacctatg attttctcta cttacttccc
     2461 cctcagtgtt tttatcttgc cttcaatttc attttcaaaa attgtcaatc cctttatgat
     2521 gtgcttgtaa gcatcggcta tattctcgta ttcgtccatt tcaaacagac catctcgcca
     2581 cattgctttg agggaattga gatttgtttc aaattcttta ttagttttcc ttacgaagga
     2641 aagtatattc ataagagcca caatggctct tggaatgtct gcttctgtta gttgtttcga
     2701 ttctggaaaa gatatcatac cggtataggt gggacccgct ccaatgtact tgttcattac
     2761 atcgtatgcc ctgttcttgg cttttttctt tgccttaaga agctcgggtg attttttctg
     2821 aagaagatcg tctatttttt cctcttttaa atcattctta tccattattt tatcctcttt
     2881 tcaatatcag aaataacttg ccaaattttt ctgtaatatg cttcttgtgc ttctttcaag
     2941 gagatttttg gctcttcaat cacagactcg ggttcttcaa cgaccctttt ctgtggcggt
     3001 tcaaaatcct ggccttcata gataccattc acccaagatg gatgatttga cgggtctgtg
     3061 accatatccc aagtgatgag ataaaaatcc tcattgacat aaccgttctc gttcacggag
     3121 ccaagaccac gggaagaaat acccattcta ccctctttga taagagtctt tgcaatattt
     3181 cccattggtg tatcaagaac ttttgaccta ccaatgacat ttgggccatc ccattccaat
     3241 ttctctgtca ggatagcaat tttatccgga ttgatctctg gatttggtgg atgcccaagt
     3301 tctccccaaa gactcctgtt taccagtctc tcttccagct tactaatttc tctctcaaga
     3361 atatgtcttg aatatattct tttattgttg ttctcttgat ctgccgtact gtaaattcca
     3421 gtaatgtaga gagacttctc cttcttttca gactcctcca caataaattt aatatcgtga
     3481 gagagctcgg ttataagctt catctttttc tcctcccagt tttatacata aaaaatgtgt
     3541 gaactaacac aactttctgg caatattata taaattactc ttcaccacca accgtcttga
     3601 gaatacgaat gggcgtggtc ttgataacat tgataacctg tgcaattctc tgattgttgg
     3661 ggtactttac tacaagttcg tgtgcaagag acatggcctt cgcaaacttt tctggtgtat
     3721 ttgccattgt gagataatat ttcaatcttt tgaggccctg tgtaatactg cctgcatccc
     3781 ttgtgggatc ataggtgccg ggaacagatc ctcctacagg cgggtaaagc ttctgatcgc
     3841 ctgccgaaaa ttcctgtggc tttggctcat caaaaaccgc ttctttcaaa tacatatcaa
     3901 ttttttcttc gatcttcttc attttttctt cttttcctcc gctagttttt gtaccacttg
     3961 cttttgtttt ggtggttcga tatcctttgt caaaccaagt ttacttttta agtattcatt
     4021 cttggcctgt ttaacaacat tcttaactat ttcttttgca gaaataaaat catcgttctc
     4081 aaaggaatcc aaagcttttt taatatcttt ctcgtccata aacacactcc ttatttttta
     4141 gccgctttct ctctttctct ttcggctttt gctctttcct gataactctc tattcttttt
     4201 tgcagagact caatatgtgc cgttcctctc ttgcgaatct gctctttctt gtcagaatca
     4261 tccgtatctt caatcgcttt tctcatcaat ttacgggagt cccttatttg tttttgcaaa
     4321 agttgaatag tatcagaata atctgcttct acaagactaa gatacttgtt cataattctg
     4381 tcaatcttca ttagatacac ctctgatata tttataatta tttatatatt tttctacaag
     4441 aatttctgta tttctgttcc acggatattt gtaggaggct ttttgtagtc tttgtttttt
     4501 cttggcctcc ttctccgctg ctttcttctc ggccctcgcc gcggcccttt gctccggtgt
     4561 ggtcaaatat atacgccttt tattgaatcc agcattcaaa ccttgtactc ttgaaccgta
     4621 tggcctccag cctaatttcc acagtttgag atttatagta ccatcttcgt atgtatttgt
     4681 ttcagagtga tattctccat ttatatactc ctgatgatta tgttccaaca tctccaaccc
     4741 atttgatgtc tgaagaacat gagaaaatgt tattgcactc ataaaacaat tcggacctga
     4801 taatgtatct ggatcgccct ttcttgctgg catacccaat accattctag cattatcata
     4861 tccaatgaca tctgataatt tatcatagtc cacaagttct ctcattcttt tggtatatat
     4921 tctatccaac ttttccctgg ctgattctga tgccgccaaa acaccttttt tcgcaatagt
     4981 ttcagccgtc gcttctttat attcttttac caatttataa aatttatcca tatcctttat
     5041 agccttatca atgccgccgc tatttttaag aatggcatca aattcttttc tatcatcaat
     5101 tatggagtcc ttgacaccta ttgtatatcc attttccccc ggccgtgatt tcaacaattc
     5161 tctcttcttt gggtctttat gatatagaca atactttgaa gcctcgcccg ggaatccata
     5221 agagctaaaa tctcccttct ttccatattt tatactaaca gattgaattt tttctatttt
     5281 tgttcccttc cttgtaatct tcagcttatc gcctgatggg aaatttccgg ctgatggaag
     5341 atatacttct ttcccccgtg caatttctgt atcatacaga gccatttcgg caaaattttt
     5401 cataacagat tcagccgcgt ctttatcaat ttctgataat ttacaggcca aagttgcata
     5461 tgaatcacct actgcctttt ctgcatctgc tgatggtatt gatttttcat tatcaacaat
     5521 ttctaacatt ctggtcttat gtgctttgag ttctgcaaca aattcttttt tcaatttccc
     5581 agctttaaca agtttttcag aggcttcaat tgttctatca agtgctttat tatttgaaac
     5641 agagtgcttg agatattcca aggagttctt ccctccctta ttatcaatcg ggtttccctt
     5701 atcatctatt ggcatatata attttttgaa accaggctcc aatgcggcca gagggccctc
     5761 ggaaaatatt tctgcaacat atttattgtc tttagcatca agtcctgtca ttcctaaatt
     5821 tggttttgat gatgtagaaa gtgtctttgt ttttccttct tcacctactt ctgcctttaa
     5881 aaatacaccg atatcctcaa aatattttgc tatattccgt gcaaccaggc cggagcctat
     5941 gagtttctta ctatcgtgat gtaattcacc aacaagatca acattcaata ctccagttgc
     6001 tcctatacgc aatccaaaat cattcttgat tttttctgct ttagctttct tttctgatgg
     6061 cttcgctttg tcaagattta taagttgctg taaaagatcg tcatatattt gttttatttc
     6121 tttacctttc acatgcattt tgagaagttt attggttttg atcttgtcgc tgatataaga
     6181 tacattagac ttcatatcag aagtaggaac ttccttttct ttggtgggct tcttctctgg
     6241 cttttctggt tttttctctt tctcttttgt gggcttcttt tcaataatat ttttccaatg
     6301 attatgaaca tcagcaaccc aagaaggaac aattccacca tattttttaa caatggagtt
     6361 gagaaattcc ttggcggctt ctggtttcat tgcatcaact acatcatgaa gccttgaaga
     6421 tttcctatca tcgggaatat tggatgcttc gattttatca aatgcgggaa tttttatact
     6481 atcatctttt ttgataggct ccgtagtacc ttttgcttct tcatcctttt cgtgagcaat
     6541 tggtttggag gctttgtctt taagcttgat gatattatct tttactgcct gagcatatac
     6601 accttctgga gatctctgta ttacataatt ctgttcgcct ggtgaccagc tattccattt
     6661 tttaatttgt tctggtttta tcttattttt aggcctttcc tcggattttg gttccggttt
     6721 tattacactt ttttgtttgg gaacaagttt ctttgcctca aaaatttgat ccagaaattc
     6781 ttcatttact tgatggaaaa aatttctaag tttcatcaat ttattttatc tcctccacgg
     6841 gaactccttc ttctggcgct ggtgagccag cgcccgtttc tccagcacca aattcctttt
     6901 caccaccaag ctcttcacta cccatttctc cttccatacc cggctccggt ggtggttgga
     6961 atagtttatc cttgtctttc agacatctta tattttcttc tatttcctca tccgtccatc
     7021 caagatattt cttcatcaag aagtatttag aaaattcctg atttccagca agagcattgt
     7081 agtttgaatg gcgaacttca acaaatccct gttccagaga ttctttgtaa tgtgacggtg
     7141 gaaccatatt gatagtcaac atatctttct caataccata ctgtttcttc aatcctctga
     7201 atgcaagatg cattaagaac aagtccttga attcatcaca gaaccttttc tgatgcttct
     7261 caagaaattt tgcccatttg acttcatcac gggaaatttc accagtatgg gaaccaccaa
     7321 caacaatctc ggcttccctc ttttcctgcc cagctgtaac acgagaagcg ggatatttta
     7381 atgctctata aagttttcgt gcaaaatagt aaatgtcatc cagttcggag aagcccgcgg
     7441 ggttaccacc tacggtttca atggatgacc ctcttccatc agcagattgt ggaaggaaga
     7501 agttttcaag aatggaaaga acttccggtt cctgtgatag ctgtcctgtt tgtggatcat
     7561 aggtttggcg cttgataaat tttgtcttaa ttttctcgac aaatttcatc gccttgtctt
     7621 ttggcatatt acccgtatca atcttgaaca caaacctttc aggtgcacga atgatacgat
     7681 aaatgatgac agatgtttca agaagcttca actgattgta aggaactctg gccttctcaa
     7741 gatacccgta aacttcacct tttgttcgtc cataaattcc atagttaata tacccaatct
     7801 gttctggatt aaacacaaca accttcggat ctttttccgc ttcttccctg tttattggtc
     7861 tttttgtatt gggagcaaga tactggtaat aataaagaat ctgtcctgtc atcggatcat
     7921 agacataatc cattgtttcc gatggaagtt tcttgattgc ttggatgcct tgtgatggtg
     7981 ccttggtatt gattattctt tcataataaa ccctgccgtc aatatagtag gttctcataa
     8041 ggtcatcaat aatcctgtct atttcaattc tcctgtaaaa gaggtcatca aattccttgt
     8101 aaagattctt gataacattc ttgttttcag atagtttatt gtctgttatg atcaaactga
     8161 aaactctgtt attattatct gttagggtcg attcattgac agcatcctca ataacatcac
     8221 caatttctgg atattccgcc atctttctgt attcgatgat ttttgcaact tctgtctcaa
     8281 agaccttgtt gatatacctg ttatagaagg tattcagatt tccataacca agagaaccat
     8341 agccggaaat gagcatatag tcctcaacac cttctccttt tgttgcctcc acttctgact
     8401 tcatgggaat atcacccttt cccacgaata ccttcataga ttcatctaat cttacatctt
     8461 catccttctt acctcgcaaa aaatcatacc aagccatatt attcctctat ctgagctttc
     8521 tcttcttcct gttttttcaa ttcaataact ctcaattgcc tctgcctttc ctcgacaaat
     8581 ttcgtcaaat caataccgat attctttcca attacatgcg ccacaacttc cgtccaaata
     8641 gtctcatttc ttaactgctc ctgcgcttgc tgaagtgcta ctgtgcgatt atgcaactcc
     8701 tgtatcatca acaaagttct ctttggaata caatcgctta caattttgac accctgatcg
     8761 ctatccaacg gatttggcgt aaaaatattc tctacaaaat tggggcattt tcttatatcc
     8821 ccaaataatt catacaaccc acaaataacc tttcctgata ctgggtcaat tggttcacaa
     8881 gttcctttca ttagttttct ccactattat ttatcttttt cataatatca ttatgtcttt
     8941 ttatttcatc gagccttgat ataactacaa aacccattga tcttaatgta ttgacgattt
     9001 tatc
//
LOCUS       DAOTQL010000067         8825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6935_length_8825_cov_5.021209, whole genome shotgun sequence.
ACCESSION   DAOTQL010000067 DAOTQL010000000
VERSION     DAOTQL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6935_length_8825_cov_5.021209"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1316
                     /locus_tag="P5293_05085"
     CDS             216..1316
                     /locus_tag="P5293_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRR49310.1"
                     /translation="MNQLAKYIMGISIALIICFLAWYFSNILIYILVSAVLSIIGKPL
                     KNLLLKIRLGKHFMPNSLAAGLTLMLLVAVFFSLFFLITPLVGQLMSNISNIDFQSLG
                     KDLSPMLDQFNALLHKWFPALDPAFKVEDVVINQFDGLVDVGVITNIFNSLTTIIIQF
                     GLAVFVIIFISYFFIKEENMFNDMVLALFPDKYVENVSRALDSINKLLARYFIGISIE
                     AILITIINSLSLHYIAGVSWSLSVAVSFLAGVLNVIPYIGPLTGGAFGTIMAFVERGV
                     TTPGGGKFILMVALIFLIAHQIDVFVFQPFIYSNSVKAHPLEIFLVILIAGSIGGIIG
                     MLVAIPAYTVIRVFAREFFSNLKLVQKLTDNI"
     gene            complement(1374..1637)
                     /locus_tag="P5293_05090"
     CDS             complement(1374..1637)
                     /locus_tag="P5293_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRR49311.1"
                     /translation="MNIFVSSLSFRAKKEDLAELFTPYGEVTSARIIFNRETRRSKGY
                     GFVEMPNEQEGNAAISALNGSEHMGRTINVAVANDRPSKEEAE"
     gene            complement(1758..3350)
                     /locus_tag="P5293_05095"
     CDS             complement(1758..3350)
                     /locus_tag="P5293_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRR49312.1"
                     /translation="MESGKVDITSDIEAISKAPAFTFILFYLAGILTGSRFFIQSTIL
                     VKIIVPLVVLVFLIISIGFKRRSMITEFLLPVLLYLSMFTAGWINIEYSNLLNNSAKF
                     SKVANIQVLNAQCKITGKILEKERSYQTEARLIDYNEKIILYIDKNVDRKSFDIGDTL
                     ECIIRPNRINNYLNSTFDYARYMEKQGFFTTSYVKASQVAVRKASKLSLKEKIIKIRE
                     NYIDYLLKNKHYVHEKAVLIALTIGDKSFIDGNTKKAFSNSGTMHLMAVSGLHVSFIY
                     SLLSSILGFMGMSRFAKIIRFIIVSLTVWTYTALVGFTPSIMRAALMATIYELSSIIE
                     REGSSLNTLSVSALIITIAQPESLFELGFQLSFASLLAIIFIHPIIVKLNPSQNIIVK
                     YLWNTISMSLACQIGTSIITIYNFRFVPTYFLITNLIAIPISGLILYLAAMHLFALWV
                     GLFHEQIMNIIIHLTRILIYTVNRIESLPLAIIDCRVNNGQAVILSALLFISVLGIPK
                     DKYMRRLSIKALIITFIISLLK"
     gene            3473..4678
                     /locus_tag="P5293_05100"
     CDS             3473..4678
                     /locus_tag="P5293_05100"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRR49313.1"
                     /translation="MILLVLNCGSSSIKYQVLDMKSDSDYSLMAKGIVERIGLDKGTL
                     THKVNGRPNYEVEMLIENHTVGIKQVLNILVDPIQGVLNSLQDIQAVGHRVAHGGEFF
                     SESRLIDSDVIEKIEKLCELAPLHNPANLLGIRAIETLLPGMKQVAVFDTSFHQTMPN
                     YAYMYPLPYEYYTKYKIRRYGFHGTSHQFVAKKGCLLTGLDYNTSKIITCHLGNGGSI
                     TSIKNGKSIDTSMGFTPLEGVIMGTRCGDIDPGIITFIQEREGLSASEMNKILNKKSG
                     FLGVFGKSSDARDIEDAAAKGDERANLALNILYYRVLKYIGAYTAVMNGVDLIVFTGG
                     IGENDPHLREMVGLRLKYMGLEFDVDSNKNARGRDVIISKSGSKVKMAAVTTNEELVI
                     ARDTMAIVS"
     gene            4720..5625
                     /locus_tag="P5293_05105"
     CDS             4720..5625
                     /locus_tag="P5293_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRR49314.1"
                     /translation="MISSFEQIFDNLRNRPKKRLVAAWGVDDHTINAVKLAIEAGIVD
                     ATLVGDEKRIREACSKENIDPSIFRIVPNDDELRAVSMAVDLINAGEGDILMKGLCST
                     DKYMRAILNKEKGLLPPKAVLSHVSVLSNPNYHKLLVLSDIAVIPAPDLTQKIAMTNY
                     VVKTAHALGIAKPKVAILCATEQMLPNMPACIDAAIISKMAERGQIAACYVDGPLALD
                     VAISQEAVAVKNLVSPVAGDADCLVFPNLEAANVFYKTNTQLCSGTRLAAMVAGAKAP
                     CVLSSRSDSIDTKLNSIALAALTSN"
     gene            5633..6703
                     /gene="buk"
                     /locus_tag="P5293_05110"
     CDS             5633..6703
                     /gene="buk"
                     /locus_tag="P5293_05110"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HRR49315.1"
                     /translation="MAYKILVINPGSTSTKIAVFEDLHELFTETLRHTTDELLPYKRI
                     TDQFEFRKRVILSALESHGVALDSISVIMGRGGLLKPVKSGVYEVNDAMLEDLKKGGG
                     EHASNLGAIIAADIASSIPNCKAYIADPIVVDELMPIARIAGHPLFERVSIFHALNHK
                     AIGRRHAASLNRRYEDINLIIAHLGGGVSVGAHRKGVVIEVNNALNGEGPFSPERSGT
                     LPALPLAELCFSGKYNLSEVTKMIVGEGGMVAHLGTNSFNLIEKMVQEGNEKAILLSD
                     AFVFNVAKAIGACAAVLKGEVDAILLTGGIANNLRLMSQIEEMVKFIAPVKVYPGEDE
                     MFALAENGYFVLTGKTKSMAYI"
     gene            6732..8003
                     /locus_tag="P5293_05115"
     CDS             6732..8003
                     /locus_tag="P5293_05115"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRR49316.1"
                     /translation="MRVRDLVVIGGGAAGMFAAASAASNGISVLLLEKMDRTGRKVRI
                     TGKGRCNITNTKPWQEFSVHIHPNPNFLKPSFFSFSNIDTINFFEKLGLHCVVERGDR
                     VFPASGVASDVVDTLMKHLDSLGVEVICNANVRNIIHQDGRVKGVIYEKGNKLYTENA
                     EAIIVATGGLSYPLTGSTGDGYNFADDLGIKVNKCFPSLTALKPDFDYTLLKWLILKN
                     VSLSLFVNGQLVQEEMGELEFTDNGIEGSIGFRVSRKAVQSLINHDKVYLVLDLKPAL
                     DKSLLIDRLTKDFNSEKSVNKVLRNYMPGQLIETFISSIPNKLISEGNSIENKINSIA
                     SKMKGWQMNITGYTSYERAVVTAGGVSLNEIIAKKLSSKRYPNLFFAGEVLDLDGDTG
                     GYNLQIAFSTGYAAGREAAYLIKKERGGSSE"
     gene            complement(7964..8515)
                     /locus_tag="P5293_05120"
     CDS             complement(7964..8515)
                     /locus_tag="P5293_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49317.1"
                     /translation="MNIVVFSVNNTGFYFKTDNTLVKGSKDFFVPDIVERLYVSPSFI
                     VKINKSAKCVSLNYAERYYNETGVGCVLYPLNLIENSTLLSSGIALSLDYSAYTSENF
                     MDKHKLSGDIVFKFGSNQFVFPTGNDVLHKVNLAIERITRYCTLKSGDLVFIELGGMN
                     EISISEEISIIYSELPPLSFFIR"
     gene            complement(8512..>8825)
                     /locus_tag="P5293_05125"
     CDS             complement(8512..>8825)
                     /locus_tag="P5293_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRR49318.1"
                     /translation="TIATLEVLEAQLERYSDTLINDIDFLAEFSTKSKSLDYAGRIIL
                     NDNEVPTFNFGKYRGKSVESVFKSDPSYYSWMMNGDFTLDTKQVITELKIKFEKSNNP
                     Q"
BASE COUNT         2843 a         1350 c         1687 g         2945 t
ORIGIN      
        1 tgaagaaagt ttaaaaccca tttttttcat ttcaggaatt ctgtctttaa tcagggaagg
       61 aagccacatt ccttcattag cagaaagtaa agagatatta actaataaga ataaagttat
      121 taaaagcttt ttcatgtgaa taaattaaat aagtatttaa gtctgtgtca caaaaatata
      181 aaaatttctt acttttgtca ataaagccat ttaggatgaa tcagttggca aaatatatta
      241 tgggtatctc aattgccctg attatctgtt tcttagcatg gtatttcagt aatattctga
      301 tttatattct ggtttcggca gtcctttcaa taatagggaa gccattaaaa aatctgttac
      361 ttaaaattag attaggtaaa cattttatgc caaacagtct ggctgctgga ttgacattaa
      421 tgttgcttgt tgctgtattt tttagccttt tctttttgat aacaccttta gttgggcagc
      481 ttatgtcaaa tattagtaat attgattttc agtcattagg taaagatctt tctcctatgt
      541 tggatcaatt taatgctttg cttcataaat ggtttcctgc tttggaccca gcattcaaag
      601 tcgaagatgt tgttataaat cagtttgatg gtcttgtgga tgttggagta ataactaata
      661 tttttaactc attgactaca ataattattc aatttggttt agctgtattt gttattattt
      721 ttatttcgta tttttttatt aaggaagaaa atatgtttaa tgacatggtg cttgctcttt
      781 ttcctgacaa atacgtcgaa aatgtctcta gagctttgga ttctattaat aaacttttgg
      841 ccaggtattt tatcggaatc agcattgaag ccattttaat aactattatt aattctcttt
      901 cactgcatta tattgcaggt gtgagctggt ctttgtcagt agccgtctct tttcttgccg
      961 gggttttgaa tgtaatacct tatataggac ctctaacagg gggtgctttt ggaaccataa
     1021 tggcatttgt ggaacgtgga gttacaacgc ctggaggagg aaaatttatt cttatggtag
     1081 cattgatttt tttaattgcc catcaaatag atgtatttgt ttttcaacca ttcatatatt
     1141 ctaatagtgt aaaggcgcat cctcttgaaa tttttcttgt aatacttatt gcagggagta
     1201 ttggagggat aatcggcatg ttagtagcca ttcctgctta tactgttata agagttttcg
     1261 cacgagaatt tttttcaaat ctcaaacttg tgcaaaagtt gactgacaac atttaaaaaa
     1321 aagagggatc tttgtttcaa agaaccctct tttttctgac acatcgaata tctctattct
     1381 gcctcttctt ttgaaggacg gtcattagct acggcaacgt tgattgtacg tcccatatgt
     1441 tctgaaccgt taagtgctga aatagctgca ttgccttctt gctcatttgg catttcaacg
     1501 aagccatatc cttttgatcg acgggtttct ctgttgaaga taatcctggc agaagtaact
     1561 tcgccataag gtgtaaatag ttcggctaaa tcttccttct tagcacggaa acttaaactt
     1621 gaaacaaaaa tgttcatgga aacggtagta aataattaat aagtaaatgt ttagttaacg
     1681 tcacaaagtt aaataaaaaa ctgaaataca aaatttttat tctaataatt tttcaataaa
     1741 ataattttag cagtttatta ttttaaaaga gatattataa atgtgataat cagagctttg
     1801 atagacagtc ttctcatata cttatctttt ggaataccta atacagatat aaataataaa
     1861 gcagacaata ttacagcttg accgttgttc actctacagt ctattatggc taaagggagg
     1921 gattcaatcc ggttcactgt atagataagt attctggtta aatgaataat tatattcata
     1981 atttgttcat gaaatagccc aacccataaa gcaaataagt gcattgcagc aagataaaga
     2041 ataagaccag aaataggaat agcaatcagg tttgtaatta aaaaataagt tggcacaaaa
     2101 cgaaaattat aaatagttat aatagatgtc cctatttggc atgcaagaga cattgaaatg
     2161 gtattccata gatatttaac tatgatattt tgtgagggat ttaatttaac aatgattggg
     2221 tgaataaata taattgcaag aagagaggca aatgagagct gaaaaccaag ttcaaaaagg
     2281 gattcaggtt gagctatcgt tattataaga gcgctgacag aaagagtatt taagctgctg
     2341 ccttctctct ctattattga tgacagttca taaatagttg ccatcaaggc tgctctcatt
     2401 attgaaggtg taaatccaac caaagcagta taagtccaga cagtcagtga tacaattata
     2461 aatcttataa ttttggcaaa cctgctcatc cccataaatc ccaatatgga cgaaagaaga
     2521 gaataaataa agctgacatg cagtcctgag acagccatca gatgcatagt tcctgaattt
     2581 gaaaatgctt tttttgtatt accgtcaata aaagatttat ctccaatagt gagtgcaata
     2641 agaacagcct tttcatgaac ataatgttta ttcttcaata aataatcaat gtaattctct
     2701 cttatcttaa taattttctc tttgagggat agcttagatg ccttacgaac agcaacttga
     2761 ctagctttaa cataagatgt tgtaaagaac ccctgttttt ccatgtacct tgcataatca
     2821 aaagttgaat ttaaatagtt gttaattctg tttggtctta taatgcattc aagagtgtcg
     2881 ccaatatcaa aagattttcg atcgacattt ttatcgatat aaagaattat tttttcgtta
     2941 taatcgataa gacgtgcttc tgtttgataa cttctctctt tttccaagat ttttcctgta
     3001 attttacact gagcattaag cacctgaata tttgcaactt ttgaaaactt agcactatta
     3061 tttaaaaggt ttgaatattc aatattgatc caacctgctg taaacatgga caggtaaagt
     3121 aaaacaggaa gaagaaactc tgtgatcatg cttcttcttt taaagccaat tgatataatt
     3181 aaaaaaacta atacaacaag aggcactata atctttacca aaattgttga ttgaatgaaa
     3241 aatctgcttc ctgtaagaat ccctgcaaga taaaacagta taaatgtaaa tgcaggagct
     3301 ttgcttatgg cctcaatgtc agaagtgata tctaccttcc cactttccat cgtcctcatt
     3361 ttgggtaaaa atagattaat ttttacaaat attggcttta aatgagcaat tattaataac
     3421 tttgtaaatt ctaatttcag atttttactg atttttttaa ttttgataac atatgatttt
     3481 attggttcta aactgcggaa gttcttcaat caagtatcag gttcttgaca tgaagagtga
     3541 ttctgactat tctttaatgg caaagggaat tgtagaaaga attggtcttg acaaaggaac
     3601 acttacccat aaagttaatg ggaggcccaa ttatgaagtt gaaatgctca ttgaaaatca
     3661 cactgttggc attaaacagg ttttaaatat tctggtagat cccatacagg gagtcttgaa
     3721 ttctcttcaa gatattcaag ctgtaggaca ccgtgttgca catggaggtg aattttttag
     3781 cgaaagcaga ttaatagatt ctgatgttat tgaaaagatt gaaaaacttt gtgaactggc
     3841 tcctctgcac aatcctgcca acttactggg gatacgcgct atagaaacac tccttccagg
     3901 catgaagcaa gttgctgttt ttgacacttc atttcatcaa accatgccta attatgcata
     3961 tatgtatcca cttccttatg agtattatac aaagtataaa attagaaggt atggatttca
     4021 tggtacaagt catcagtttg tggcaaaaaa aggttgtttg ttgacaggtt tggactataa
     4081 tacatcaaaa attataactt gtcatcttgg taacgggggt tcgataactt ctataaaaaa
     4141 cggcaaatcc attgatactt caatgggttt tactcctctt gaaggggtaa tcatgggtac
     4201 cagatgtggt gatatcgatc ctggaattat cacttttatt caggaaaggg aagggctaag
     4261 tgctagtgaa atgaataaaa ttttaaataa aaaaagtggc tttttaggag tatttggaaa
     4321 atcttctgat gcgagggata tagaagatgc tgcggccaaa ggagatgaaa gagccaatct
     4381 tgctcttaat atattgtatt acagagtttt aaaatatatt ggagcttata ctgcagttat
     4441 gaatggtgta gatttaattg tttttactgg aggcatcgga gaaaatgacc cacatcttag
     4501 agagatggtt ggattacgtc ttaaatatat gggacttgaa tttgacgttg attcaaataa
     4561 aaatgcaaga ggaagagacg tgataatttc taagtctggt tccaaggtca aaatggcagc
     4621 agttactacc aacgaagagc tcgttatagc cagggatact atggctattg tgtcataatg
     4681 tgaaattaat gataaattaa ataataaaat agttataata tgattagttc ttttgagcaa
     4741 atttttgaca atttgagaaa caggcctaaa aaaaggcttg tagcagcatg gggtgtggat
     4801 gatcatacaa taaatgcggt taaacttgct attgaagcag gtattgtaga tgcaacgctt
     4861 gtcggtgatg aaaaaagaat ccgggaagca tgttctaaag agaatatcga tccttcaatt
     4921 tttagaattg ttcctaatga tgatgaactc agagctgttt caatggctgt tgatttgatt
     4981 aatgccggag aaggggatat cttgatgaag ggattatgct ctaccgacaa gtatatgagg
     5041 gctattctta acaaggaaaa aggattgctt ccccctaaag ctgttttaag tcatgtttcg
     5101 gttctgtcca atcctaatta tcataaattg cttgtattaa gtgatattgc agtaattcct
     5161 gcaccagatc ttactcaaaa aatagcaatg acaaattacg tcgttaagac tgctcatgct
     5221 ttaggtattg caaaaccgaa agttgcaata ttgtgtgcaa cagagcaaat gcttccaaat
     5281 atgcctgctt gtattgatgc tgccataata tcaaaaatgg cagagagagg tcagatagct
     5341 gcttgttatg ttgatggacc acttgcactt gatgttgcta tttctcaaga ggctgtagct
     5401 gtcaaaaatc tggttagtcc tgttgctgga gatgcagact gtctcgtatt tccaaattta
     5461 gaggctgcga atgtctttta caaaacaaat actcaactct gttcaggaac acgacttgct
     5521 gcaatggttg caggtgctaa agcaccatgt gttctttcca gcagatcgga cagtattgac
     5581 acaaaactta attcaatagc tttagctgcg ctaacttcaa attaatacag ttatggcata
     5641 taagatttta gtaataaatc caggctctac atcaactaaa attgcagtat tcgaagattt
     5701 acatgagctt tttacagaga cccttcgtca cacgacagac gaactcttgc catataaaag
     5761 aattacagat caatttgaat ttagaaagag agtcatttta tcagctttag aaagtcatgg
     5821 tgttgcttta gattccattt cagttattat gggaagagga ggtctgttga aacctgtaaa
     5881 atctggagtc tatgaggtca atgatgctat gcttgaagat ttaaagaaag gaggtggtga
     5941 acacgcaagc aatctcgggg caattattgc cgcagatatt gcaagttcta ttcctaattg
     6001 taaagcctat attgctgatc ctattgttgt agatgaatta atgccaatag ctcgaattgc
     6061 tggtcatcca ctttttgaga gagtttcaat atttcatgct cttaatcaca aggcaattgg
     6121 aagaagacat gctgcgtctc taaacagaag atatgaagat atcaatctga taatagctca
     6181 tcttggagga ggtgtttctg ttggtgcaca tagaaaaggg gtagttatag aggttaataa
     6241 tgccttgaat ggagaaggac cattttcgcc tgaaagatct ggtacattac ccgctttacc
     6301 tcttgccgaa ctttgctttt cgggtaaata taatctttcg gaggtgacaa aaatgattgt
     6361 tggtgaaggg ggaatggtgg ctcatcttgg tacaaattct ttcaatctta tagagaaaat
     6421 ggttcaagaa ggtaatgaga aagctattct tcttagtgat gcttttgtat ttaatgttgc
     6481 taaggcaata ggtgcatgtg ccgcagtttt gaaaggagag gtagatgcta ttcttcttac
     6541 aggcggaatt gctaataatc tcagattaat gtctcaaatt gaagagatgg ttaaatttat
     6601 cgcacctgtc aaggtttatc ctggtgaaga cgagatgttt gcattggcag aaaatggata
     6661 ttttgtgctt acaggaaaaa caaaatctat ggcatacatt taatagttaa catttttttc
     6721 tcttttttga aatgagagtc agagatcttg ttgtaatagg cggaggagct gcgggaatgt
     6781 ttgctgccgc ttcagctgct agcaacggga tctctgtcct tttacttgaa aagatggata
     6841 ggaccggcag gaaagttcgt ataacaggta agggaaggtg taatattact aatactaagc
     6901 catggcaaga attttctgtt catattcatc ctaatcccaa ttttttaaaa ccatcctttt
     6961 tttctttttc aaatatagat accattaatt tttttgaaaa attagggtta cattgtgttg
     7021 ttgaaagggg tgacagggtg tttccagctt ccggtgttgc ctctgacgtt gttgacacat
     7081 tgatgaaaca ccttgacagt ttaggtgttg aagttatttg caatgctaat gtaagaaata
     7141 ttattcatca ggatggtcgt gtaaaaggtg tcatttacga aaaaggcaac aaactttata
     7201 cagagaatgc tgaagcaatt attgtagcta cgggcggttt gtcttatccc ttaacggggt
     7261 caactggtga tggatataac tttgcagatg atcttggaat taaagtcaat aaatgttttc
     7321 catcacttac tgctttaaaa cctgattttg attatacact tttgaagtgg ctaattctaa
     7381 aaaatgtatc actttcactt tttgtaaatg gacaattagt acaggaagag atgggagagc
     7441 ttgagtttac agacaacgga attgaaggat ccataggttt tagagtgagc agaaaagcag
     7501 ttcagtcact gataaaccat gacaaagtat atctggttct tgacctgaag cctgctttag
     7561 ataagtcttt attaatagac aggcttacta aagattttaa ttctgaaaag tctgttaaca
     7621 aagtactaag gaattatatg cccggacagt taattgaaac atttatttct tctattccaa
     7681 ataagttaat atcagagggc aattctattg aaaataaaat aaattcgatt gcctcaaaaa
     7741 tgaagggctg gcagatgaat attacaggat atacctcata cgagagggca gttgttacag
     7801 caggaggagt gtctcttaat gaaattattg ccaaaaaact atcttcaaag aggtatccaa
     7861 atcttttttt tgcaggagag gttcttgacc tggatggaga tactggtggt tataatcttc
     7921 agattgcatt ttcgactgga tatgctgcag ggcgagaggc tgcttatctg ataaagaaag
     7981 aaagaggagg aagctcagag taaattatcg atatctcttc tgatatagat atttcattca
     8041 ttcccccaag ttctataaaa actaaatctc cacttttgag agtgcaatat cttgtaattc
     8101 tttctattgc aaggttgact ttgtgtaaaa catcatttcc agttgggaaa acaaactgat
     8161 tactaccaaa tttaaatact atgtctcctg ataatttatg tttgtccatg aaattttcgg
     8221 aagtatatgc tgaataatcg agggagagag ctatacctga ggaaagtaaa gtggagtttt
     8281 ctattagatt aagtggataa agaacgcaac ccactcctgt ctcattatag tatctttcag
     8341 cataattcaa actgacacat tttgcggact tgttgatttt tacaataaat gacggggaaa
     8401 cataaagtct ttctacaata tccggaacga aaaagtcctt tgatcctttt acaagagtat
     8461 tgtcagtctt gaaataaaat cccgtgttat taactgaaaa tactacaata ttcattgagg
     8521 attattactt ttttcaaatt tgattttaag ttcagtaata acttgcttgg tatcaagtgt
     8581 gaaatctccg ttcatcatcc acgaatagta gcttggatcg gacttaaata cagattcaac
     8641 gcttttgcct cgatattttc caaaattaaa tgtgggaact tcattatcat tgagaataat
     8701 tcttcctgcg tagtcaagag attttgattt tgttgagaat tcagccaaga aatctatatc
     8761 gtttattaaa gtatcagaat atctttcaag ttgagcttca agaacttcaa gagtagctat
     8821 agtgt
//
LOCUS       DAOTQL010000068         8806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6959_length_8806_cov_5.079191, whole genome shotgun sequence.
ACCESSION   DAOTQL010000068 DAOTQL010000000
VERSION     DAOTQL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6959_length_8806_cov_5.079191"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..476)
                     /locus_tag="P5293_05130"
     CDS             complement(111..476)
                     /locus_tag="P5293_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRR49319.1"
                     /translation="MDQIEDFRTYRAMMNKKILDQNTLFFKRFFNLDTRTYENGALSS
                     QTKEMLGLVASMVLRCDDCIKYHLEKCFELKVSRDQLFEIFSVANLVGGSIVIPHTRR
                     ALEYWDELEIRLKDNSSVL"
     gene            527..1468
                     /gene="mdh"
                     /locus_tag="P5293_05135"
     CDS             527..1468
                     /gene="mdh"
                     /locus_tag="P5293_05135"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRR49320.1"
                     /translation="MSKVSVIGAGNVGATCAYAMSLMQVASEVILLDVKPGLAEGKAI
                     DMMQTAKHLKFETRITGVTNDYKATENSDVIVITSGIPRKPGMTREELIGTNASIVNE
                     VVSNSLKHSPNAILVIISNPMDTMTYLTLKSSGLPKNKVIGMGGMLDSSRFVYYLSKA
                     LDAPASDVAGLVIGGHGDTTMIPLIRFANLRGIPVSELTTKDVLDQIVEDTMKGGATL
                     TKLIGTSAWYAPGANAAALVRAIVHDERKVYPCCVYLEGEYFQNDICIGVPCIIGKNG
                     WEKIIELNLNLEERTLFQQSAEAVRTTNNVLKDLKLV"
     gene            1517..1590
                     /locus_tag="P5293_05140"
     tRNA            1517..1590
                     /locus_tag="P5293_05140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1551..1553,aa:Ala,seq:ggc)
     gene            1736..2434
                     /locus_tag="P5293_05145"
     CDS             1736..2434
                     /locus_tag="P5293_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRR49321.1"
                     /translation="MEVKKTPKADLENKKLMFREIGLIIALAVSLFAFEWKSTDKQET
                     TLQADTAVTIEEEMIPITQETPPPPPPSMEIPLVTDELQIVDNDIKVNTDLLINTEDD
                     KNLGVDIKNYVVGGTGKTEAVEEEEIPFQIVEEKPKFMGGDENTFTKWVFERLVYPEV
                     AKENGIQGRVTLQFVVDTDGSVRDVKVIRGVDPALDKEAVRVVSSSPKWTPGRQRDKP
                     VKVRYIFPVIFQLR"
     gene            2515..3684
                     /gene="hflX"
                     /locus_tag="P5293_05150"
     CDS             2515..3684
                     /gene="hflX"
                     /locus_tag="P5293_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRR49322.1"
                     /translation="MTSEKCVLIGIITSNDDESKVTEYLEELEFLAETAGAVCEKKFI
                     QKMEKSNNKTYVGKGKIEEIKKFITDNNIKLVIFDDELTPSQLRNIETELKCQVLDRT
                     NLILDIFAQRAKSAYAKTQVELAQYQYLLPRLTKMWSHLERQRGGIGMRGPGESQIET
                     DRRIILDKIAKLKEQLKKIDKQMSSQRGNRGSLVRIALVGYTNVGKSTIMNLLSKSDV
                     FAENKLFATLDTTVRKVVIENVPFLLSDTVGFIRKLPHQLVESFKSTLDEVREADILM
                     HIVDVSHPGFEEQIKVVNETLAEIGAKEKPQYMVFNKIDLYKYVPQDEYDFGPKKPEN
                     YSLEELKKTCMKKVNNPCIFISAKERIQIDKLKNDIYTMVAEIHAGRYPFNNFLW"
     gene            complement(3665..4804)
                     /locus_tag="P5293_05155"
     CDS             complement(3665..4804)
                     /locus_tag="P5293_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRR49323.1"
                     /translation="MKEKFIYFLFLFFFSSIVTFAQHRVRDYGIQIGVFNTGKYNAIT
                     DVSGVSVGHLTKIEGDDIRTGVTAIIPHQANIFRNKVPAAVYIGNGFGKLAGISQIME
                     LGNIETPIILTNTMSIAPALDALITYTLMQKGNEGVVSVNGVVGETNDSELNNIRARK
                     ISSQDVLSAINNAKTGPVEEGCVGAGTGTVCFGFKGGIGTSSRVLPATLGGYTVGVIV
                     QSNYGGNLVVDGFKAGEFFEKYPYRKDILQDVDGSCMIVVITDAPITSRNLERLAKRA
                     IMGLAKTGGIASNGSGDYVIAMSVAPQNLVNELQPFYTPEELQNDAMSPLFMATIEAT
                     EEALLNSLFTAKSTRGFNGREVPRLPVEQIIKYSRRQLDTTKENY"
     gene            complement(4801..5532)
                     /locus_tag="P5293_05160"
     CDS             complement(4801..5532)
                     /locus_tag="P5293_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRR49324.1"
                     /translation="MVFLHGYLETLYIWDDFISLLPDLIRVIAIDLPGHGLTGSNMEE
                     NSIPFMADVVNSVLDLCNVESAYIVGHSMGGYVAIEAARRYYDRFDGLILMHSTPWAD
                     SDEKRLDREREIELIRNAKLSLIVKTAIPKMFAKENLRRMDEKIYEIVELAETHDPEG
                     IIATIRGLMHRPDNLEFLKKYRKPLLLFFGMNDIHIPVDKAVILQHELSDAKSIFLLH
                     SGHNGFLEEPSIVCDELLRFIGLNV"
     gene            5685..6227
                     /locus_tag="P5293_05165"
     CDS             5685..6227
                     /locus_tag="P5293_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49325.1"
                     /translation="MKSRIMGKKQIILIFISILLIMCLAMTGCSKDTDTISFVGIWDI
                     DTSSFTIVYNEQVDSTHPEAMMFLKNNRENIVRSIMKPEQIIFRNTGVDFVYKSFNPE
                     LVYTGNYTVYEIYANIYNRVFPSGITAAACNSRKLELCYTTEYMMSILKNMLTEEDPD
                     YNVFESLIISFKGVGVYYKN"
     gene            complement(6224..>6817)
                     /locus_tag="P5293_05170"
     CDS             complement(6224..>6817)
                     /locus_tag="P5293_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRR49326.1"
                     /translation="PVAFDHDCREGICGTCSLYINGRAHGPDNEITTCQLHMRKFNDG
                     DTITVEPWRSAAMPVIKDLIVNRNAFDQILQAGGYISVSTGGVPDANSILIPKEKAEE
                     SMDAAACIGCGACVATCKNGSAMLFISSRVSSLALLPQGRVEAANRVKAMVDKMDELG
                     FGACTNTRACEMECPKHVSISHIARLNREFLLAKLKD"
     assembly_gap    6818..6917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6918..7012)
                     /locus_tag="P5293_05175"
     CDS             complement(<6918..7012)
                     /locus_tag="P5293_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRR49327.1"
                     /translation="MDFTLKVWRQKDAKTKGHFETYKVTNISPDTS"
     gene            complement(7027..>8806)
                     /locus_tag="P5293_05180"
     CDS             complement(7027..>8806)
                     /locus_tag="P5293_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HRR49328.1"
                     /translation="LGYNVKVFCISDSPRRAHSIAAQGGINAAKNYPNDNDSVYRLFY
                     DTIKGGDYRSREANVYRLAEVSNLIIDQCVAQGVPFAREYSGMLDNRSFGGAQVSRTF
                     YARGQTGQQLLLGAYAALNRQIHLGNVKSYTRHEMLDLVLINGEAKGIIARNLVTGEI
                     ERHGAHAVVLASGGYGNVFFLSTNAMNSNGSAAWQCYKKGAYFANPCFAQIHPTCIPV
                     HGEQQSKLTLMSESLRNDGRIWVPKKLEDVKKLRNKEITPSQIPEEDRDYYLERRYPA
                     FGNLVPRDVASRAAKERCDAGYGVNETGLAVFLDFKSAIERLGEHVIAERYGNLFQMY
                     EKITDVNPYKEPMMIYPAIHYTMGGLWVDYNLQTTIPGLYAIGEANFSDHGGNRLGAS
                     ALMQGLADGYFILPYTIGDYLASKIQVPKTDINDPAFVEAESNVKKKVDKLLSINGTQ
                     TVDFYHKKLGHIMWEYVGMARNESGLKKAIEEIRVLREQFWHDVKVPGTFSELNQELE
                     KALKVADFMEIGELMALDALDRKESCGGHFREEMQTEDGETLRDDNNFMYVSAWEYAG
                     DGVEPNLHKEPLNYEFVKPSTRNYKD"
BASE COUNT         2976 a         1710 c         1482 g         2538 t
ORIGIN      
        1 taattttttt aatgccaaaa taccctcttc gtgcaaaaca gaaattatcc atttggaaca
       61 agcgcgcttt gatttaaaaa taaaagtaat gtcttctgta aaatatctta tcataagaca
      121 gaagaattat ctttaagcct gatttctagt tcgtcccaat attccaatgc acgccgtgta
      181 tggggaatta caatcgaacc accaactaaa ttggcaacag aaaaaatttc aaacaattga
      241 tctctggaaa ctttcaattc aaaacacttt tctaaatgat atttaatgca atcgtcacat
      301 ctcaaaacca tagaagccac aagaccaagc atctcttttg tctgagaaga gagagcacca
      361 ttttcataag tccttgtgtc taaattaaaa aaacgtttaa aaaataaagt attctgatca
      421 agaatttttt tattcatcat ggcacgatat gtgcggaaat cttctatttg gtccatataa
      481 atttttttac aaaattaatc aattctaata ataatttttt tataaaatgt ctaaagttag
      541 tgtaatagga gccggaaatg taggtgcaac ctgcgcatat gctatgagcc tcatgcaagt
      601 agcatcagaa gttattcttc ttgacgttaa acccggattg gctgaaggta aagccataga
      661 catgatgcaa acggcaaaac atctcaaatt tgaaacaaga ataaccggcg taacaaatga
      721 ttataaagcc acagaaaatt ctgatgttat tgtgataaca tcagggatac cgagaaaacc
      781 cggtatgaca agagaagaac taattgggac aaatgcatca attgtaaatg aggtagtaag
      841 caattcgctg aaacattcac ctaatgcaat tttggtaata atctctaacc caatggacac
      901 gatgacatac cttacactta agagctcggg tttaccaaaa aacaaagtaa taggaatggg
      961 aggcatgctt gacagcagcc gctttgtata ttaccttagc aaggctcttg atgcaccagc
     1021 aagtgatgtg gcaggtttag taattggtgg acatggagat acaacaatga taccactgat
     1081 cagatttgcc aaccttagag gaatacctgt ctcagaacta actacaaaag atgttttaga
     1141 tcaaattgtc gaagacacaa tgaaaggtgg agcaacgttg acaaagctca taggcacatc
     1201 tgcctggtat gcaccaggag ccaatgctgc agcattggtc agagcaatag tacatgatga
     1261 aagaaaagta tatccatgct gtgtatatct tgagggtgag tacttccaaa atgatatttg
     1321 tataggggtt ccatgtataa tcgggaaaaa tggttgggaa aagataatag aactgaacct
     1381 taatcttgaa gaaaggacgc tttttcaaca aagcgcagaa gctgtaagaa caacaaataa
     1441 tgtactaaag gacttgaaat tagtttgata gttcattccg gagatggaac aatttttgta
     1501 tgaagaataa taaggagggg tattagctca gttggctaga gcgtttgcat ggcatgcaag
     1561 aggtcatcgg ttcgattccg atatgctcca caaaagtttt caacaaatta ataagcgcat
     1621 aactggctgg tttaagtata cttgacaata tttagcaaaa gtttatccac aatagctttt
     1681 gtcaaatagc aatttattat tatttttaca cactcaaaat cgcatagata aagttatgga
     1741 agtaaagaaa acacctaaag ccgatctgga aaacaaaaag ttaatgttta gggagatcgg
     1801 gctaataatt gctcttgcag tttccctgtt tgcctttgag tggaaaagca cagacaagca
     1861 agaaacaacc cttcaagctg atacagctgt taccattgaa gaagagatga ttcctataac
     1921 tcaagaaaca cctcctcctc ctcctccatc aatggaaata cctctcgtta cagacgaatt
     1981 gcaaattgtc gataatgata ttaaagttaa cacagattta ttaatcaata cagaagacga
     2041 taaaaatctg ggagtggaca tcaaaaatta tgtagtcggc ggcacaggta agacagaagc
     2101 agttgaagag gaagagattc cattccagat tgtagaagaa aagccaaaat ttatgggtgg
     2161 cgatgaaaat acttttacaa aatgggtttt tgagaggtta gtttatcctg aagttgccaa
     2221 ggaaaatggc atacaaggac gagtaaccct gcaatttgta gtagatacag acgggagtgt
     2281 cagagacgtt aaggttatac gaggtgtcga tcctgcattg gataaagaag ctgtaagagt
     2341 cgtttcttca tcacctaagt ggacacccgg aaggcaaaga gataaacctg taaaggtccg
     2401 ctatatattc ccggtaattt tccagttaag ataaaaatac ttacataaat attatataaa
     2461 tgctgcttct tacagatgca gccttttaca tataataaac aacattaata ttttatgaca
     2521 tcagagaaat gtgtacttat tggcatcata acatcaaatg atgatgaatc taaagtaacg
     2581 gaatatcttg aagagctgga atttcttgct gaaaccgctg gagcagtatg tgaaaaaaag
     2641 tttattcaaa aaatggaaaa atccaataac aagacttatg tggggaaagg gaaaattgag
     2701 gaaatcaaaa aatttataac agataataac ataaaattag taatttttga cgatgaactc
     2761 acaccatcac aacttagaaa tatcgaaaca gagttaaagt gtcaagtact tgacagaact
     2821 aatcttatct tagacatatt tgctcaaaga gcaaaaagtg cctatgcaaa gactcaagtt
     2881 gaattggcac aataccagta tcttttgccg cggctgacca aaatgtggtc acatttggaa
     2941 agacaaagag gtggtatagg tatgagaggt ccgggagaaa gtcaaattga aactgacaga
     3001 agaattatcc ttgataaaat tgcaaaactt aaagaacagc tcaaaaaaat tgacaaacaa
     3061 atgtcctcgc aaagaggaaa cagaggcagt ctggtgagaa tcgctcttgt gggctacaca
     3121 aatgtgggta aaagcacaat aatgaatctt ttgtcaaaga gtgacgtatt tgcagaaaat
     3181 aagttgtttg caacacttga cactacagtc cgtaaagtgg taattgaaaa tgtcccattt
     3241 ctcctaagcg atacagtagg gtttataaga aaattgcctc atcaattagt ggaatcattt
     3301 aaaagcacac ttgacgaggt tagagaagca gatatattaa tgcatatagt agacgtctct
     3361 catccaggat ttgaagaaca gataaaagtt gtgaatgaaa ctctggcaga aattggtgca
     3421 aaggaaaagc cacagtacat ggtttttaac aagattgatc tatataaata cgttccacag
     3481 gatgaatatg actttgggcc aaagaaacct gaaaactaca gtcttgaaga gttaaagaaa
     3541 acatgtatga aaaaggtaaa taatccatgt atatttatat cagcaaaaga aagaattcaa
     3601 attgacaaac ttaaaaatga catatacaca atggttgctg aaatccatgc agggagatat
     3661 ccattcaata attttctttg gtagtatcta actgccggcg agaatattta attatctgtt
     3721 ccacaggtaa tcttggaact tctcttccat taaaacctcg cgttgatttt gcggtaaaaa
     3781 gcgaatttaa taaagcttct tcagttgctt ctatagttgc cataaaaagt ggtgacatag
     3841 cgtcgttttg aagttcttcc ggtgtataaa aaggttgaag ttcattgacc aaattttgtg
     3901 gtgcgacaga catagctatt acataatctc cgcttccatt tgaagcaata ccgccagtct
     3961 ttgccaaacc cataattgct cttttggcca atctctctaa attgcgtgaa gttatgggag
     4021 catcagtaat aacaacaatc atacaagaac catctacatc ttgtaaaata tcttttctgt
     4081 aaggatattt ttcaaaaaat tcgcctgctt taaatccatc tactacaaga tttccaccgt
     4141 aatttgactg aactattact ccgacagtat atccacctaa agttgcaggc aaaacacgcg
     4201 aagatgtccc aataccacct ttaaaaccaa aacatacggt acctgtacca gctccgacac
     4261 atccctcttc taccggtccc gtcttagcat tatttatagc cgatagaaca tcctgcgatg
     4321 atatttttct tgctctgata ttgtttaact cactatcgtt agtctcccct actacaccat
     4381 taacagaaac gacaccctca ttgcccttct gcataagagt ataagttatt aatgcatcta
     4441 aagcaggagc aatgctcata gtatttgtca aaatgatagg tgtctcaata ttgccaagtt
     4501 ccattatctg agaaatgccc gctaacttgc caaaaccatt accaatataa accgcagcag
     4561 gaactttgtt cctgaaaata ttggcttgat gaggtattat tgcagtaacg ccggttctta
     4621 tatcatctcc ttcaatcttg gttaaatgac cgacgcttac tccggaaaca tctgttatag
     4681 cattatattt acctgtatta aaaacgccaa tttgaatgcc ataatctctg actcgatgtt
     4741 gagcaaaagt aactatagac gagaaaaaaa ataaaaataa aaaataaata aatttctctt
     4801 tcatacattt aatccgataa atcgcaaaag ttcatcacaa acaatagaag gttcctcaag
     4861 aaaaccgtta tgaccggaat gtagcaaaaa aattgacttt gcatccgaaa gttcatgttg
     4921 taatatcacc gccttatcaa caggtatatg tatatcattc atgccaaaaa ataacagtaa
     4981 tggttttcga tatttcttta aaaattctaa attgtcaggt ctatgcatca aaccccgaat
     5041 tgtggcaata atgccctctg gatcatgtgt ttcagcaagt tcgacgattt cataaatttt
     5101 ctcatccatt cgccgcaaat tttcctttgc aaacatttta ggaatagcag ttttaacaat
     5161 caacgataat tttgcattcc ttataagttc tatctccctt tctctgtcca acctcttctc
     5221 atcagaatca gcccatggag ttgaatgcat aagtatcaat ccatcaaaac gatcataata
     5281 gcgccttgca gcttctatag caacatagcc acccatcgag tgaccaacta tatatgctga
     5341 ctcaacatta cacaaatcaa gaacagaatt aacaacatct gccataaaag gtatagaatt
     5401 ctcttccata tttgaaccag ttaaaccgtg cccaggaaga tctattgcta taactctgat
     5461 tagatcaggc aacaaactta taaagtcatc ccaaatgtaa agtgtctcaa gataaccatg
     5521 aagaaatacc aaacatttcc tcccccgtat cgtatcagaa atatgtacgg gaataccatt
     5581 acaagtagca aaaaattcca ttatccgata tttactcaaa tttaattctt tttgaagatt
     5641 ttttatacat ttgtaaagat acgggggcat tttttcaaat aaaaatgaaa agccgcataa
     5701 tggggaaaaa acaaataatt cttattttta tttctattct actgataatg tgtctggcaa
     5761 tgacaggttg cagcaaagat acagacacta tcagttttgt cgggatatgg gacatagata
     5821 cctccagctt tactattgta tacaatgagc aagtcgattc aacacatcct gaagcaatga
     5881 tgtttctcaa aaataacaga gaaaatatag ttagaagtat aatgaaacct gagcagatta
     5941 ttttcaggaa tacaggcgta gattttgttt acaaatcttt caatccggaa ctcgtttata
     6001 caggtaatta tacagtttac gagatttatg caaatattta caacagagta ttcccttccg
     6061 gcatcactgc tgcagcatgc aattctcgga aacttgagtt atgctataca actgaatata
     6121 tgatgtctat tctaaaaaat atgttgactg aagaagaccc cgattacaat gtttttgaga
     6181 gtcttataat cagttttaaa ggagtcggag tctattacaa aaactaatcc ttaagtttcg
     6241 ccaacaaaaa ttctctgttc agtctggcta tatgagaaat agaaacatgc tttggacatt
     6301 ccatttcaca tgctcttgta tttgtacaag caccaaaccc caattcatcc attttatcta
     6361 ccatagcctt gactctgttg gcagcttcta ccctgccctg aggtaaaagt gcaagtgaac
     6421 ttacccttga tgaaataaaa agcatcgccg agccattttt gcatgtagct acacaagctc
     6481 cacaacctat acaagccgca gcatccatac tctcctcagc cttttcttta ggaattaata
     6541 ttgaattagc atcagggact ccacctgtag aaacagaaat ataaccacct gcctgtaata
     6601 tctgatcaaa agcatttcgg tttacaataa gatccttaat aacaggcata gctgcagatc
     6661 tccaaggttc aacagtaata gtgtcaccat cattaaactt acgcatatga agttgacaag
     6721 tagttatctc attgtcaggt ccgtgagctc ttccattaat atataacgaa caagtgccac
     6781 atataccttc acgacaatca tgatcaaaag caacaggnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnngat gtatctggtg aaatattcgt taccttatat gtttcaaaat
     6961 gacctttagt tttagcatct ttctgacgcc atacttttag tgtaaaatcc ataatgaaaa
     7021 tgtttcttaa tctttatagt ttctagtaga aggcttgaca aattcataat tgagaggttc
     7081 cttgtgcaaa ttaggttcaa ccccgtctcc agcatattcc catgcactca catacataaa
     7141 attattatca tccctaagag tctcgccatc ttcggtctgc atctcttctc taaaatgacc
     7201 accacacgat tctttcctat ctaatgcatc taaggccatc aactctccta tctccataaa
     7261 atctgcaact ttaagagcct tttcaagttc ctgatttaat tcagagaatg tacccggtac
     7321 tttaacgtcg tgccagaact gctcccttaa aactctgatc tcttctattg ccttcttaag
     7381 cccactttcg ttcctggcca ttccaacata ttcccacata atatgtccaa gtttcttgtg
     7441 ataaaaatca actgtttgag tcccatttat tgaaagcagt ttgtctacct tttttttgac
     7501 attactttct gcctcaacaa aagcaggatc atttatatct gtctttggta cctgaatctt
     7561 ggaagcaaga taatcgccta ttgtataagg caaaataaaa taaccatcag cgagtccctg
     7621 cataagagct gaagcaccta aacgattccc gccgtgatca ctaaaattag cttcacctat
     7681 tgcatacaaa ccaggtatag ttgtctgaag attataatct acccaaagtc ctcccatagt
     7741 atagtggatt gcaggatata tcatcatagg ttccttgtat ggattgacat cagtaatctt
     7801 ttcgtacatc tgaaaaaggt tgccatatct ctctgcaata acatgctcgc cgagtctttc
     7861 tattgcactt ttaaaatcaa gaaaaacagc aagtcctgtc tcattgacac catatccagc
     7921 atcacatctc tccttagcag cccttgaagc aacatcacgt ggtactaaat tgccaaaagc
     7981 aggatagcgg cgttcaagat aataatcacg atcctcttca ggtatttgag atggagttat
     8041 ctctttgttc ctcagttttt taacatcttc caatttcttt gggacccaaa ttcttccatc
     8101 attacgtaat gattcgctca tcaaagtaag tttactttgc tgttcgccat gaactggaat
     8161 acatgttgga tgaatctgag caaaacaagg attagcaaaa taagcaccct ttttataaca
     8221 ttgccatgct gcagaaccat tgctgttcat agcattagta gaaagaaaga aaacattgcc
     8281 ataaccccca gaagcaagaa ctactgcatg tgctccatgc ctttctatct cccctgtaac
     8341 caaatttctg gcaataatac ctttcgcttc tccattaatc aaaaccaaat ccagcatctc
     8401 atgacgtgtg tacgatttaa cattcccaag atgtatctgt cggttcaaag ccgcatatgc
     8461 tcctaataaa agttgctgac cggtttgccc tcttgcataa aatgttcgac ttacctgggc
     8521 accaccaaaa gagcgattat ccaacatacc gctgtattct cttgcaaagg gaactccttg
     8581 agctacgcat tggtcaataa ttaaattact tacctcagca agacgataaa catttgcctc
     8641 cctgcttcta taatctcctc cctttatagt atcataaaaa agacgataaa cagaatcatt
     8701 atcatttgga taattttttg cagcattgat gcctccctga gccgctatag agtgtgctct
     8761 tcgaggagag tcactgatac agaaaacttt aacattatat ccaagt
//
LOCUS       DAOTQL010000069         8788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_6982_length_8788_cov_5.192259, whole genome shotgun sequence.
ACCESSION   DAOTQL010000069 DAOTQL010000000
VERSION     DAOTQL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6982_length_8788_cov_5.192259"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="P5293_05185"
     CDS             complement(<1..433)
                     /locus_tag="P5293_05185"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HRR49329.1"
                     /translation="MSTDNTNLRVDVRSVIKFKNPDLLRYIPGFIIDWVERLICQDEI
                     NNILDKYGNLKGVPFATATLRELNATYSVSGIDKIANKGRYIIVSNHPLGGFDGIILM
                     DIFGNYFDNKIRFVVNDLLSAVEPLKPIFLPVNKFGKQSVEI"
     gene            501..1910
                     /gene="rpoN"
                     /locus_tag="P5293_05190"
     CDS             501..1910
                     /gene="rpoN"
                     /locus_tag="P5293_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRR49330.1"
                     /translation="MLKQGLQQKLVQKLSPLQIQTIKLLELPTLELEQRIKKELEENP
                     VLDEAVESDDTEEASSGNISLSEYSTDEQIPSYKLYANNQGKDLKPQYNVFSVKESFH
                     QNLISQLGYSQMDPRERNIARFIIGSLDDDGYLRRDLDSLVDDISFRLGITTNERELE
                     QILSKIQEFEPVGVGARNLQECLLLQIRAKNKTPEQQLAEKIISDHFNEFTKKHYQKI
                     IAKLSITEEQLKSAIDEILKLNPKPGGQIDDSYNEQSQQVVPDFVLELKDGELVMTMP
                     RFSVPELKVNKRYADLLLASTKSGSKSGKEAATFVKQKLDSAKWFIEAIKQRHNTLQN
                     TMNAIIEFQHDYFIDGDETKLRPMVLKNIAEKTNLDISTISRVVNCKYIQTHFGIFPL
                     KYFFSEGLLTESGEEVSTKEIKNILMESINQEDKKHPLTDEELVTVLSKKGYKVARRT
                     VAKYREQLNIPIARLRKEL"
     gene            1981..3558
                     /locus_tag="P5293_05195"
     CDS             1981..3558
                     /locus_tag="P5293_05195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRR49331.1"
                     /translation="MKDKVDLIFYNAEVYTVDSMFSKCNAVSVKNGLIVATGSDDEIL
                     NSYRADKYIDLKGKYLYPGFNDAHCHFISLGEGMLKCDLRGATSFDEIVERLRNFRTN
                     NLTSFIIGEGWDQNLWHSKSFPSNEKLDIFFPDIPVVLTRIDFHAVIANSAAIKMAGL
                     IPGDPSIPQREALIRNGKFTGLFLENTADRIKNALPKLSGSVLENVILAAEGECFKNG
                     LTSVSDAEEELSRILVMDSLYSRSKLRIRSDVWLTANDENLNYFKKPYQKERFRVGTL
                     KLYVDGALGSRGALLKSPYSDDSSTSGIRVTSEENLKEYCKWAFERGFQVATHCIGDL
                     ANQEALGIYGSILKGENDRRWRIEHAQVVDPEDIGLFRKYSVIPSVQPTHATSDMVWA
                     VDRLGERIKYAYAYKSLLSQLGWLPSGTDFPIEKVNPIATFFSAVYRQNADFKPDEGF
                     QMENALSKEEAIRSMTIWPAKATFEERVKGSIEKGKYADFVLLDRDLMTVIKKEVLST
                     KVIMTIIGGEVVYSLLP"
     gene            complement(3540..4193)
                     /gene="upp"
                     /locus_tag="P5293_05200"
     CDS             complement(3540..4193)
                     /gene="upp"
                     /locus_tag="P5293_05200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRR49332.1"
                     /translation="MRIVNIGEQNSILNNFIAEIRDRIIQKDSLRFRRNLERIGEIFA
                     YEISKELEYTTKSIQTPLGVAACNTLSDSLILASIIRAGLPLHNGLLSFFDASQNAFV
                     AAYRKYGKDNKFTIQIEYASTPSVEGKVLILTDTMLATGSSVVLAYNRLCDISKPKHS
                     HIVCPIASVYGIEYLSKHLPHKETTLWVAAVDEELTVKSYIVPGLGDAGDLAYGSKL"
     gene            complement(4227..4916)
                     /gene="radC"
                     /locus_tag="P5293_05205"
     CDS             complement(4227..4916)
                     /gene="radC"
                     /locus_tag="P5293_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRR49333.1"
                     /translation="MNRRLSIKEWQEDERPREKLMAKSATALDDSELIAIILGSGTRN
                     ATAVDLARELLAACNNSLRELSKLNIDNLTKRCGIGPAKAITIAALFEIAKRYSVTEN
                     KSIIQIQSSSTAAGIISPILKDLPHEECWVLYLNRANRLISKERISIGGVSATVIDVK
                     IVIKSALEKLASSLILVHNHPSGNLQPGEHDKMQTKILKEAAALFDIALLDHLIIAGD
                     EYFSFADNGII"
     gene            complement(4986..5240)
                     /gene="rpsT"
                     /locus_tag="P5293_05210"
     CDS             complement(4986..5240)
                     /gene="rpsT"
                     /locus_tag="P5293_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRR49334.1"
                     /translation="MANHASAKKRARQSEKRREHNKYYARTTRNALKALRNTTDKAAA
                     EAMLPKIYSMLDKLAKVNIIHANKAANLKSGIAAHVNKLS"
     gene            complement(5263..5334)
                     /locus_tag="P5293_05215"
     tRNA            complement(5263..5334)
                     /locus_tag="P5293_05215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5299..5301),aa:Glu,seq:ttc)
     gene            complement(5398..6378)
                     /locus_tag="P5293_05220"
     CDS             complement(5398..6378)
                     /locus_tag="P5293_05220"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HRR49335.1"
                     /translation="MKNFNFLLLIIIGSLLASCKGGPKLMPNISGKAGEIVVVISKGD
                     WESDPGIAIRSVLAQEHPYLPQREPLFTLINIPENAFSNIFQIHRNIIIIHTGVNVKE
                     PTIAYQENVWAAPQTVVTISTANAKDAVDLINRESDKLINVFLQAERNRNIANAKKYE
                     ERGLRDVVTATFGGSPYFPKGYTLKKRTDDFIWISYETTYTIQGVFIYRYPFRDSSDF
                     TPSRILEIRNEVLARNVPGPLENTYMTTNMTIEPGFRWVKYNKRTFAELKSLWEVQND
                     FMGGPFVDHIFLDKEKKNLIILEAFVYAPRYDKRNYLRQVESIIYSFEFF"
     gene            complement(6391..7716)
                     /gene="rseP"
                     /locus_tag="P5293_05225"
     CDS             complement(6391..7716)
                     /gene="rseP"
                     /locus_tag="P5293_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRR49336.1"
                     /translation="MVILIKILQVVAALSLLVLVHEFGHFVFARLFKIRVEKFYLFFD
                     PWFSLFKFKPKGSDTEYGIGWLPLGGYCKISGMIDESMDKEALKEEPKPWEFRSKPAW
                     QRFFVMFGGVFFNFILAILIYSATLFTWGEEYLKNEDAIYGVQCNELAREIGFKNGDK
                     IISVDGQPVEKFQDIQIDIARNQAGSVKVLRDGAEVTININPNYIPAILNTPGMFTLR
                     VPFEILSIPDSSLNANAGLMRGDRVAEVDSQKVFIVQDIQEILKQHKGDTVNCVINRS
                     GVMIPKKLLVDTAGRIGVYLNTDIHKFFKVTEVNYSLLEAIPSGISKTVSTLTNYVKE
                     LGLIFSPKTEAYKSVGSFIAIGSIFPNSWEWDIFWNITAWLSIMLAVINLIPIPALDG
                     GHILFLFYEIITGRKPSDKFLEIAQVIGMLILLAIMFLAFGNDIARLFK"
     gene            complement(7718..>8788)
                     /gene="dxr"
                     /locus_tag="P5293_05230"
     CDS             complement(7718..>8788)
                     /gene="dxr"
                     /locus_tag="P5293_05230"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRR49337.1"
                     /translation="EVSALTANSNSKKLVKQAKEFNPQCVVIADESHYEEVYSALKYL
                     DIKVYTGEESIAEIVKSNETDIVLTAMVGFSGLKPTIEAIKSRKTIALANKETLVAAG
                     QIITTLADEYNTPIIPVDSEHSAIFQCLQGHPEDIEKILLTSSGGPFFSKNIEEIANA
                     TVEQALKHPRWAMGAKITIDSASMMNKGFELIEAKWLFKVDPCDIEIVIHPQSIIHSM
                     VQFKDGAVIAQLSHPDMRLPILYALSYPFRAELNTRRLDFAEIRNLDFFKPDYNKFPC
                     LEIATQAINKGGNIPCAMNAANETAVAHFLNGALPFSAIPKLIENVLSGTKFVANPTL
                     EEIFLTDRIAREQADIEKYKFN"
BASE COUNT         2693 a         1633 c         1714 g         2748 t
ORIGIN      
        1 caatttctac actttgctta ccgaacttat tgaccggcaa aaaaattggt tttaatggct
       61 ctactgcaga aagtaaatcg tttaccacaa aacgtatttt attatcaaaa tagttaccga
      121 aaatatccat tagtattatt ccgtcaaatc ctccaagagg atggttggag acaataatat
      181 agcgcccttt attagctatt ttatctattc ccgaaacact gtatgtcgca tttaactccc
      241 taagtgtggc agttgcaaaa ggaacgcctt taagattacc atacttgtca agaatattat
      301 taatttcatc ttgacagatt agtctctcta cccagtcaat tataaatccg ggtatatatc
      361 tcagaagatc aggatttttg aattttatta ctgatcttac atctacccgc aagttggtat
      421 tatctgtgct catagaatag cgctgtatca ggatgcgaag ttaaagaaaa atcatatctt
      481 tgcataaacg ctccaggaat atgttaaaac aaggtttaca gcagaaatta gtacagaaac
      541 tttctccatt acagatacag acaattaaac tgctggagct tcccaccctt gaacttgaac
      601 agaggattaa aaaggaactt gaggagaatc ctgttctgga cgaggctgtg gagagtgatg
      661 atacagaaga agcgagttcc ggcaacatct ccttaagtga atacagcaca gacgagcaga
      721 taccatctta taaactctac gccaataatc agggtaaaga ccttaagcca cagtacaatg
      781 ttttctcggt taaagaaagc tttcatcaga atctaatatc tcagttggga tacagtcaga
      841 tggaccctag agaaaggaat attgcacgtt ttattatcgg tagcctggat gatgacggct
      901 atttacgaag agatcttgat tcattggtcg atgatatttc tttcagactc gggataacaa
      961 ccaatgaaag ggaactggag cagattttgt ccaagattca ggaatttgag cctgttggtg
     1021 ttggtgcaag aaatttgcaa gaatgtcttc ttttgcagat aagagctaaa aataaaactc
     1081 cagagcaaca attggctgaa aaaatcattt cagaccattt taacgagttt acaaaaaaac
     1141 actatcagaa gataattgcc aaactttcaa taactgaaga acagttaaaa tctgcaatag
     1201 atgaaatatt gaagttaaat ccaaagcccg gaggtcagat tgatgattca tacaatgagc
     1261 agtctcaaca agtagtgcct gatttcgtac ttgagctgaa agacggagag ttggttatga
     1321 cgatgccgag attttcagtg ccagagttga aagttaacaa gcggtatgct gacttgttac
     1381 ttgcttcgac caagtccggc agtaagtccg gcaaggaagc agccacattt gtcaagcaaa
     1441 agttggattc ggctaaatgg tttatcgagg ctataaagca gaggcataat actctgcaaa
     1501 ataccatgaa tgccattatt gaatttcagc acgactattt tatagatgga gatgagacca
     1561 agcttaggcc catggttctc aaaaatattg cagagaagac taaccttgac atttcgacta
     1621 tttcgagagt cgtgaactgt aagtatattc aaactcactt tggaattttt ccgttaaaat
     1681 atttcttttc tgagggactt cttacagaga gtggagagga ggtgtctacc aaagagatta
     1741 aaaacattct catggagagt ataaaccagg aagataaaaa acatcctcta accgatgagg
     1801 aacttgtaac tgtactctca aaaaaaggct ataaagttgc gcggagaaca gtagcaaaat
     1861 acagagaaca gcttaatatt cctattgcaa gactcagaaa ggagctttaa atactgtcat
     1921 ttaatgaaca aaaatctgtt tttaacaacc tttttcgcac tcataatttc ttcatgtcag
     1981 atgaaagata aagttgacct tatattttat aatgctgaag tatatactgt tgattcaatg
     2041 ttctcaaaat gtaatgctgt ttcagttaaa aatgggctga ttgtggctac tggaagcgac
     2101 gatgagattc ttaacagtta cagagccgat aaatatatag atcttaaggg taagtatctg
     2161 taccctggat ttaatgatgc tcattgtcac tttataagtc ttggagaggg gatgttgaaa
     2221 tgtgatttaa gaggagccac atcctttgat gaaattgttg agaggcttag aaatttcagg
     2281 acaaataatc ttacgtcctt tatcattggt gagggctggg atcaaaatct atggcatagt
     2341 aagtcatttc cttcaaatga aaagctagac atattttttc ctgatattcc cgtggttctt
     2401 acaaggatag attttcatgc agttatagca aacagtgctg caataaaaat ggccggttta
     2461 atacccggag acccttcaat accacagaga gaggcattaa tcagaaatgg aaagtttacg
     2521 gggctgtttc tggagaatac tgcagataga ataaaaaacg ctcttccaaa actcagcggc
     2581 agtgtcttgg agaatgtcat actggctgca gaaggagaat gttttaagaa tggtcttact
     2641 tctgtttctg atgctgagga ggagctttca agaatacttg tgatggattc gctttattct
     2701 cgttccaaac taagaattag atctgatgta tggcttacag ctaatgatga aaatctgaat
     2761 tatttcaaga aaccatacca aaaagagcga ttcagggtag gtactttgaa gctgtatgtg
     2821 gatggagctc tcggttcaag gggagcatta ttgaaatcac catattcgga tgattcaagt
     2881 acctcaggaa taagagtcac tagtgaggaa aatcttaaag aatattgtaa atgggctttt
     2941 gaaagaggat ttcaagttgc cacacattgt atcggagatc ttgctaatca ggaagctctt
     3001 ggaatttatg ggtctatatt gaagggagag aacgacagaa gatggagaat tgaacatgct
     3061 caggttgttg atcctgaaga tattggtctt tttagaaaat attcagttat tccatctgta
     3121 caaccaactc atgcaacatc agatatggtg tgggcagttg acagattggg tgaaagaata
     3181 aaatatgcat atgcatataa gtcacttctc agtcagcttg gctggttgcc ctctggaaca
     3241 gattttccaa tagaaaaggt taaccctata gctacttttt tttccgctgt ctatagacaa
     3301 aatgcagatt ttaagcctga tgaaggcttc cagatggaaa acgccctgag taaagaagag
     3361 gccattagat ctatgacaat ctggccggca aaagctactt ttgaagagag agtgaaaggc
     3421 agcatcgaaa aagggaaata cgcagatttt gtcctgcttg acagggatct catgacagtt
     3481 atcaaaaaag aagtactttc cacaaaggtg attatgacaa ttataggagg tgaagtggtt
     3541 tacagtttac ttccgtaagc aaggtctcct gcatctccta atccgggcac tatataagat
     3601 ttaactgtaa gttcttcatc tactgctgct acccaaagtg tagtctcttt atgaggaaga
     3661 tgcttggata ggtattcaat accatataca cttgctatgg ggcagacaat atgactgtgc
     3721 tttggtttgg aaatatcgca aagtctgtta tatgcaagta ctactgacga accggtggca
     3781 agcattgtat ctgtaagaat aagtactttc ccttcgacag aaggagtact tgcgtattct
     3841 atctgaatag taaatttatt gtcttttccg tattttctat aagctgcaac aaatgcattc
     3901 tgtgaagcat caaaaaaaga gagcaagcca ttatgaagag gtaggcctgc tcttattatg
     3961 cttgcaagta taagtgaatc ggaaagagtg ttgcaagcag caacccctaa aggtgtctgt
     4021 atactttttg tagtatattc caattctttg cttatttcgt aagcaaagat ttctccaatt
     4081 ctttctaaat ttcttctgaa cctgagactg tctttttgta tgattcgatc tctaatttca
     4141 gctatgaagt tgttaagaat tgaattctgt tctcctatat taacgattct catgtatttt
     4201 tcaatttggc caaaggtata aaataatcag attattccgt tatcagcaaa actgaaatat
     4261 tcatctcctg caataatcag atgatccaga agtgcaatat caaataacgc agctgcctct
     4321 ttcaagattt tggtctgcat cttgtcgtgt tcgccgggtt gtaagttacc ggaaggatgg
     4381 ttatgtacaa gaattagtga actggcaagt ttttcaagag cacttttaat aactatttta
     4441 acatcaatta ctgtagcaga gacacctcca atgctgattc tttctttgga tatcaatctg
     4501 tttgctctat tcagatataa tacccagcac tcttcatgtg gaaggtcttt caagataggg
     4561 ctgataatac cagcagcagt tgaagaggat tgaatctgta taatagattt attttccgta
     4621 acagaataac gtttggctat ctcgaagagt gcagcgattg taattgcttt ggccggtcca
     4681 ataccgcatc tttttgtcaa attgtcgata ttcagttttg acaattctct cagactgttg
     4741 ttgcatgctg caaggagctc tcttgccagg tcaacagctg tagcgtttct tgttccactt
     4801 cctaatatta tggctatcaa ttcactgtcg tcaagagctg ttgcgctttt agccatcaat
     4861 ttttctcttg gtctttcatc ttcctgccac tctttgatcg ataatctcct attcataata
     4921 aagaaataaa aaagctctcc aaattttgga aagctttttt aaattcatat gcatgaatgt
     4981 aaaaactatg aaagtttgtt tacatgtgca gcaataccgc tttttaaatt tgcagctttg
     5041 ttggcgtgaa taatgttaac tttagccaat ttatcgagca ttgaataaat ctttggcagc
     5101 attgcctccg ctgctgcttt gtcggtagta ttacgtaatg cctttaaagc attgcgagtt
     5161 gttctagcat aatacttgtt atgctctcta cgcttttcgc tttggcgagc gcgttttttt
     5221 gctgatgcat gatttgccat ttcttttcta ttatattttt tgtagtcggt aggggaatcg
     5281 aacccctatt gcaagaatga aaatcttgag tcctaacctt tagacgaacc gaccatgcac
     5341 accctcccaa gattgggagt gcaaaggtaa aaattatcta cttagttcca aaaaatatta
     5401 gaagaattca aaggagtaga ttatggattc aacttgacgt agataattcc ttttatcata
     5461 tcttggcgca tatacaaacg cctcaaggat aatcagattc ttcttctcct tatcaagaaa
     5521 aatatggtca acgaaaggcc ctcccatgaa atcattctgc acttcccaca ggcttttgag
     5581 ttctgcaaat gttcttttat tgtattttac ccatctgaac ccaggctcta ttgtcatatt
     5641 tgtggtcatg taggtgtttt ctaatggccc aggaacatta cgtgctaata cctcgtttct
     5701 tatttccaat attcttgaag gcgtaaaatc cgaactgtcc ctgaaaggat acctgtatat
     5761 aaaaacgccc tggattgtat aggttgtttc gtaagagatc catatgaaat catcagttct
     5821 tttcttaagt gtatagcctt ttggaaaata tggagatccc ccaaaagtag cagtaaccac
     5881 atctctcaat cctctttctt catacttttt agcattggca atgtttctgt tcctttctgc
     5941 ttgcaaaaaa acatttatga gtttatcact ctccctgttt ataagatcta cagcatcctt
     6001 tgcatttgca gttgaaattg ttacaactgt ttgtggggct gcccaaacat tctcctgata
     6061 agcaattgtg ggctccttaa cattaacccc agtgtgaata ataattatgt tcctgtggat
     6121 ttgaaagata ttggaaaaag cattttcagg aatatttatg agggtgaaaa gaggttccct
     6181 ctggggaaga tagggatgtt cttgagcaag gactgaacgt atggcaattc ccggatctga
     6241 ctcccagtcg cctttgctta ttactacaac tatttcacct gcctttccac ttatgtttgg
     6301 cattaattta gggcctccct tgcatgaggc aagaagtgat ccgatgatta tgagaagtaa
     6361 aaaattaaaa tttttcatat tcttgttatt ttatttaaaa agtctagcaa tgtcgttccc
     6421 gaaagcgaga aacattatag caagcaaaat gagcatacct atgacttggg caatttcaag
     6481 gaatttatcg ctaggttttc tgccggttat aatttcatag aatagaaaga gaatatgtcc
     6541 tccatcaagt gcaggaatgg gtataagatt tattactgca agcatgattg acagccaggc
     6601 tgttatgttc caaaatatat cccattccca ggagtttggg aaaatactgc caatagcaat
     6661 aaagcttccg acagatttgt aagcttcagt cttcggagaa aatataagac ccagttcctt
     6721 gacatagtta gtcagggtag aaacagtctt tgaaattcct gaaggaattg cctcaagcag
     6781 tgagtagtta acctcagtaa ccttgaaaaa tttgtgaata tccgtgttaa gatatactcc
     6841 gattctccca gcggtatcaa caagcagttt tttcgggatc ataactccac tcctgttaat
     6901 aacacaattg acagtgtcac ctttgtgttg ttttaaaatt tcctgaatgt cctgaacaat
     6961 aaacactttt tgagaatcaa cttcggcaac tctgtctcct cgcatcaatc ctgcattggc
     7021 attgagcgaa ctgtccggga tggaaagaat ttcgaaaggt actctgagtg taaacatccc
     7081 gggggtattc agaattgccg gtatataatt tggattaata ttaatagtga cctcagctcc
     7141 atctctcaaa accttaacgc ttccagcttg attacgagcg atatcgatct gaatatcctg
     7201 aaacttttcg acaggttgac catcaacgga aattatttta tctccattct tgaaaccaat
     7261 ttcccgggca agctcattgc attgaacacc ataaatagca tcttcgtttt taagatattc
     7321 ttctccccaa gtaaaaaggg ttgcggagta gattaagatt gccagaataa aattaaaaaa
     7381 gacgcctcca aacatcacga aaaatctttg ccaggcaggc ttacttctaa attcccacgg
     7441 cttaggttct tctttgagcg cctccttatc catggattcg tcaatcattc ctgaaatttt
     7501 gcagtatccg cccaaaggaa gccacccaat tccatattcg gtgtcactac cttttggttt
     7561 gaatttgaat agcgaaaacc atgggtcaaa gaagagataa aacttttcga ctcttatttt
     7621 aaaaagacgt gcaaaaacaa aatgaccaaa ttcatgaaca agaaccagca atgataaagc
     7681 tgcaacaact tgtagtattt ttattaaaat caccatttca attaaattta tatttctcta
     7741 tatctgcctg ttctcttgca attctgtcag tcaaaaatat ctcctccaat gttggatttg
     7801 caacaaattt tgttccagac agcacatttt caattaattt ggggatagca gagaaaggca
     7861 gagcaccatt gagaaaatga gcaactgccg tctcattggc agcattcatt gcacaaggta
     7921 tgtttccccc tttgtttatt gcctgagttg ctatttcaag gcaaggaaac ttattgtaat
     7981 cgggtttgaa gaaatcaaga tttcgtatct ctgcaaaatc aagtcttctt gtgttcaatt
     8041 cagctctgaa tggatacgag agtgcataca agataggcag tctcatatcg gggtggctta
     8101 attgtgctat cacagctcca tctttgaatt gaaccattga gtgaataatt gattggggat
     8161 gaataactat ctcaatatca caagggtcta ccttaaacag ccacttggct tcgattagtt
     8221 caaacccttt attcatcatg ctggccgagt ctattgtgat tttggcaccc atagcccatc
     8281 tcggatgttt aagagcttgc tccacagtgg catttgcgat ctcctcaatg ttttttgaaa
     8341 aaaatggacc accggaggag gttaaaagga tcttttcaat atcttcagga tggccttgaa
     8401 gacattgaaa tattgcggag tgttctgaat caacaggaat aatgggtgtg ttatattcgt
     8461 cggcaagagt ggtaattatc tgcccggcag caaccagcgt ctctttgttt gctaaagcga
     8521 tagtttttct ggattttatt gcctcaatag ttggttttaa tccgctaaat ccaaccattg
     8581 ctgtcagaac aatatcagtt tcgttacttt ttacgatttc ggcaattgat tcttcaccag
     8641 tatatacttt aatgtccaga tatttaaggg cgctgtagac ctcttcgtaa tgagactcgt
     8701 cagctataac aacacactgt ggattaaact ccttggcctg tttaacaagc ttttttgagt
     8761 ttgagtttgc agtaagagca ctaacctc
//
LOCUS       DAOTQL010000070         8763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7000_length_8763_cov_5.499311, whole genome shotgun sequence.
ACCESSION   DAOTQL010000070 DAOTQL010000000
VERSION     DAOTQL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_8763_cov_5.499311"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="P5293_05235"
     CDS             complement(<1..649)
                     /locus_tag="P5293_05235"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRR49338.1"
                     /translation="MKRSFLLLAIVVLNLDFVFAQRENQLAFAPGEMLHYLVKYQWGP
                     FNTEVGDVVFTIEQKSDTIKPYYIAKANGKSFKFYDVFFKVRDYYESRFYPEDITPFY
                     FYRDVLEGKYRMKNYIYFLKDNNIRALYKKRDNPIRDTLHKVPTKTYDLLSLVYSIRN
                     ENISSKPVGVAFTVSFAIDGKINYVKYSYAGSEVKKVFGLGIFNTLKIEAKPVAGH"
     gene            complement(651..2150)
                     /gene="dnaB"
                     /locus_tag="P5293_05240"
     CDS             complement(651..2150)
                     /gene="dnaB"
                     /locus_tag="P5293_05240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRR49339.1"
                     /translation="MAKTYKKKENPDKEVDLISIEMGKKPPQAVDIEEAVLGALMLEP
                     EAVADVVDTLVPECFYKESNRKIFSAIISLATRHAPVDIFTVSEELNKTGDLDIVGGT
                     VYLTQLSMKIGAAAHIDYHSKILLQKYIQRELISISYQIQRDSFDDTIPVDDLLDSTQ
                     QKIFTLSDRNMKKDTQSIQYVISEAIEELQNNQIKANGLSGIPSGYTSIDRVTLGWQP
                     SDLIIIAARPSMGKTAFVLTMARNMAVDHNVPVAFFSLEMSSLLLAKRLMISETGLEP
                     DKIKGGKRLEDYEWEQLNAKLNKLVKAPLYIDDTPSLSIYEFRSKARRLVSNAGVKMI
                     VIDYLQLMTGPPEFKGMREQEVSAISRSLKAIAKELNIPIIALSQLSRAVETRGGTKR
                     PQLSDLRESGAIEQDADIVMFIHRPDYYSSAEENPELIGLTDIIIAKHRNGEVRDVQM
                     RFRHSEVKFVDTNDVKLDTEMFSNERIETFSSKMNKEEFISNSDFDINF"
     gene            complement(2154..3371)
                     /locus_tag="P5293_05245"
     CDS             complement(2154..3371)
                     /locus_tag="P5293_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRR49340.1"
                     /translation="MSSVPYILRELFFRHIAQTSPYPFGLEIEKAEGVFLYGPDGGKI
                     IDLISGVSVSNVGHANKEVIDAVKAQIELYMHLMVYGELIQAPQVEHATLLTSCLPEN
                     LNSVYYVNSGSEANEAAVKLAKRVTGRREIVSCYNCYHGSTQGVMSLMSDEELKRAFR
                     PLLPGISHINFNNFDDLRKITEKSAAVIIEPVQGEAGVILPVEGYLEALRQRCNEVGA
                     LLIFDEVQTGFGRCGKLFAFQKYGVIPDIITLAKALGGGMPLGAFIASGDLMESWQFS
                     PALGHITTFGGHPVSCAAALASLKVLLKEPWVDEVEGKSQRFVKYLRDHPLIKEIRAA
                     GLLIAVELGESGLGELAVRLLLKEGVMSDWFLSTDSAFRIAPPLCINDAEIDYAVEKI
                     LKVLDKIFSEKGR"
     gene            complement(3372..4187)
                     /locus_tag="P5293_05250"
     CDS             complement(3372..4187)
                     /locus_tag="P5293_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRR49341.1"
                     /translation="MATAKSKSNIETPLDGGTFISLQKTANEGDLSMETKLRLLYNLQ
                     QTDSKIDKIHLLRGELPLEVQDLEDDIQGLKTRLENLQTEYKEFEKLIAQKKMDLENS
                     KNLIEKYTSQQNNVKNNREYDSLSKEIEYQGLEVQHAEKIIKEKTLLLAEKKEAIAAA
                     QEAIKERTIDLENKKKELESIITETAKEEKELIKKSEEIASQIDARMLAAYQKVRCNA
                     RNGLAVVTVRRDACAGCFNKIPPQRQMDIASSKKIIVCEYCGRILVSADFEKE"
     gene            complement(4194..4973)
                     /locus_tag="P5293_05255"
     CDS             complement(4194..4973)
                     /locus_tag="P5293_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRR49342.1"
                     /translation="MTKASRIIEILESLAPLDIQEEWDNSGLCIGNAEREVTSLLLAL
                     DCTEEVLDEAVKLGAEMIVTHHPLIFRGIKQITQATFTERIIGRAIKNDIVIYSLHTN
                     LDKVIAGVSGEMASKLGLSNLSFLDPYPGGDTGLGIVGNFIEPVSFNELILRVKRVFG
                     TECVKSSRPLDKLVSRVALCGGSGASLIEAAKKSGADVFITADLSYHLFFTEPGFALM
                     DIGHFESESCVLEVIKNELLKKMATFAIHIASSNINPIYYY"
     gene            complement(4970..5308)
                     /locus_tag="P5293_05260"
     CDS             complement(4970..5308)
                     /locus_tag="P5293_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRR49343.1"
                     /translation="MPYKEFKVDKLFYTIGEVAEILGENTSLVRFWAQKFPEYIKPAR
                     NKKGNRLFTAEDLSNFKIIHYLVKERGMTLEGAAKRMKDNKTGVDRRVDVIASLNNIK
                     TILLEIAESL"
     gene            complement(5332..6288)
                     /locus_tag="P5293_05265"
     CDS             complement(5332..6288)
                     /locus_tag="P5293_05265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRR49344.1"
                     /translation="MSKYRFNKEQLKFVEDKLGVTGKIRVVVRYLLLTVLLAIFYYII
                     IAFFVNTPAEERLIKENELKAQQYREAVKKMEVLDNVIQNLENRDRDIYQSIFKSEPP
                     EIFPVNNRSVLYAQLDSSSDDQLVLMIASKVKLVESMLVDQNKKIEAISVLIKNNPDV
                     KSIPSILPIKGIEISQTGAGIGKRIHPFFKTAVDHTGLDLIASLGAQIHATADGVVSE
                     ITKTGDKGSGNVLVISHSNGYVTKYAHLGDILVRKGQPVKQGAIIARVGNSGLSFAPH
                     LHYEVILNGEVMDPVDYFFADLSPEQYREMKIIAMSSGQSLD"
     gene            complement(6303..7265)
                     /locus_tag="P5293_05270"
     CDS             complement(6303..7265)
                     /locus_tag="P5293_05270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRR49345.1"
                     /translation="MPKKRRYKFNPETLAYELHKIPMRVKFSKGFLMFLASLLTSTGC
                     YMLYTKYFGFETPKMMILLSKSEELSNRLDLMNKRFQEANNSLISLQMRDNNIYRPVF
                     GMEEIPQEVRNAGFGGVDRYSYLKNFDNSGLLERTAENLDILYKKAFVQSKSFDEVIE
                     QAKNADKLALSVPAIPPVNISSHRIRFSSGFGYRPDPFTGQYRMHTGVDLSGQVGEPI
                     YSTGNGKVVEIGYDFFGYGNFIIIDHGFGYKTRYAHLKASLVVMGKQVRRGEQIALMG
                     NTGRSKGPHVHYEVIYQGRPVNPLNYYNRDIKPEEFLAMVTPKG"
     gene            7361..>8763
                     /gene="alaS"
                     /locus_tag="P5293_05275"
     CDS             7361..>8763
                     /gene="alaS"
                     /locus_tag="P5293_05275"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRR49346.1"
                     /translation="MMTSNEIRDKFLNFFASKDHTILPSAPMIVKDDPTLMFTNAGMN
                     QFKDWFLGNTPAKYKRVADSQKCLRVSGKHNDLEEVGHDSYHHTMFEMLGNWSFGDYF
                     KKEAIEWAWELLTEIYKIDKERLYVTVFEGSKEDNISPDEEAKTQWLKFLPGDRVLFG
                     NKKDNFWEMGDTGPCGPCSEIHIDIRSTEDRKKISGASLVNKGDPHVIEIWNLVFIQY
                     NRKANGELVQLPQKHVDTGMGLERLCMIIQGKQSNYDTDLFTNMIASISRLSGKSYNE
                     SEQISVAMRVIADHIRAISFSIADGQLPSNVKAGYVIRRILRRAVRYAYTFLGIDQPF
                     LCKLVPTLVTNLGEAYPELIKQQSLIEKVIKEEEEAFLRTLDKGIKLMETLIEKNKTT
                     KVIPGKEAFTLYDTYGFPIDLSELIAKEHGYTIDLSTFEKELSKQKERSRNATSTEES
                     DWIELRPFNGVEFVGYD"
BASE COUNT         2718 a         1882 c         1468 g         2695 t
ORIGIN      
        1 cgtgtccggc cacaggttta gcctcaatct taagcgtatt aaaaataccc aagccgaaaa
       61 ctttttttac ttcagatcct gcatatgaat atttaacata attaattttg ccgtcaattg
      121 cgaaacttac tgtaaaggca acaccaacag gtttggatga gatgttttca tttcttatag
      181 aataaacaag tgaaagaaga tcatatgttt tagtcggaac tttatgtagt gtatctctta
      241 taggattatc tctcttttta tacaaagccc ttatattatt atctttaaga aaatatatat
      301 aatttttcat gcgatatttt ccttcaagta catccctgta aaaataaaaa ggagtaatat
      361 cttcaggata aaatcttgat tcataataat cccttacctt aaaaaaaaca tcataaaatt
      421 taaaactttt accatttgct ttggcaatat aatatggttt tattgtatcg cttttctgct
      481 caatcgtaaa aaccacgtct ccgacttcag tgttaaatgg tccccactgg tatttgacca
      541 aataatgaag catctcaccg ggagcaaagg caagttgatt ttctctctga gcaaatacaa
      601 aatctaaatt taaaacaaca atagctaaaa gtagaaaaga tctcttcatt ttaaaaatta
      661 atatcaaaat cggaattaga tataaattct tccttattca tcttggaaga aaatgtttca
      721 attcgctcat tggaaaacat ctcagtatcg agcttcacat cattggtatc aacaaatttt
      781 acctctgaat gcctaaacct catttgaaca tctcttactt ctccatttct atgtttagca
      841 ataataatat cagtaagtcc tattagctcc ggattctctt ctgctgaaga ataataatca
      901 ggacgatgaa taaacataac aatatccgca tcctgctcaa tagcacctga ttcgcgaaga
      961 tcgctcagtt gtggcctttt tgtaccacct ctggtctcta ctgcacgact caactgcgaa
     1021 agtgctataa tcggaatatt caactcctta gctatagcct tgagtgagcg agatattgcc
     1081 gatacttcct gctctcgcat gcctttaaat tcaggaggtc ctgtcatcaa ctgaaggtag
     1141 tcgataacaa tcatcttaac accggcattt gaaaccagcc tccttgcctt ggaacgaaat
     1201 tcataaattg agagagaagg cgtgtcatcg atataaagag gtgcttttac cagtttatta
     1261 agttttgcat taagctgttc ccattcataa tcttcaagtc tcttccctcc cttgatttta
     1321 tcgggctcca gtcctgtttc actaatcatt aatcttttgg caagtaagag agaagacatc
     1381 tcaagagaga aaaatgctac cggcacattg tgatcaacag ccatattcct tgccatagta
     1441 agcacaaaag ctgtttttcc cattgatgga cgtgctgcaa taattatcaa gtcagaaggt
     1501 tgccagccaa gagtaactct gtcgatagat gtataaccag acggtattcc acttaaacca
     1561 tttgctttta tctgattgtt ctgcaactct tcaatcgctt ccgaaataac atattgtatc
     1621 gactgagtat cttttttcat gtttctgtcg gagagagtaa aaatcttctg ctgagttgag
     1681 tcaagcagat cgtcaacagg aatagtatca tcaaaagaat ctctctgtat ctgataggaa
     1741 attgagatta actccctctg aatatacttc tggagcaaga ttttagaatg atagtcgata
     1801 tgagctgcag ctcctatttt catgctgagc tgagtaaggt aaacagttcc gccaactata
     1861 tcaagatcac cagttttatt aagttcttct gaaacagtaa aaatatctac gggagcatgt
     1921 cttgtagcaa gagagattat tgcagaaaat atctttctgt tactctcctt ataaaaacac
     1981 tcaggcacaa gagtatctac gacgtccgca acagcctccg gttcaagcat aagagcccca
     2041 agaacggctt cttcaatatc tacagcctga ggtggtttct tccccatttc aatgctgata
     2101 agatctacct ccttgtcagg attttccttt tttttataag ttttagccat taactacctc
     2161 cctttttcag aaaagatctt gtccaatact ttaagaatct tctccacagc gtaatcaatt
     2221 tctgcatcat tgatacacag tggaggagct attctgaatg ctgagtctgt cgaaaggaac
     2281 cagtcactca ttacaccttc tttcaataac aatcttacag caagttctcc caatcctgat
     2341 tctccaagct caactgcaat taacaatcct gctgccctaa tctctttgat taaaggatga
     2401 tccctgagat atttcacaaa acgctgtgac ttaccttcca cctcgtcaac ccatggttct
     2461 ttgagcaata ccttaagaga tgcaagtgct gcagcacagg agacaggatg acctccgaaa
     2521 gttgtaatat gacctaatgc tggtgaaaat tgccaacttt ccattaaatc tccggaagca
     2581 ataaatgccc ctaatggcat acccccacct aaagctttag ccaaagtaat gatatcagga
     2641 ataaccccgt acttttgaaa tgcaaaaagc tttccacaac ggccaaaacc tgtctgcacc
     2701 tcatcaaaga tcagtaaagc tcccacctca ttacagcgtt gtcgcagagc ttcaagataa
     2761 ccctcaacag gtaagattac accggcttcg ccttgaacag gttcaataat tacagctgca
     2821 gatttttccg ttatctttct taaatcatca aaattattga aattaatatg actaatcccg
     2881 ggtaaaagtg gtctaaaagc tctcttaagt tcttcatcac tcataaggct cataacaccc
     2941 tgtgtgctac cgtggtaaca attataacag cttacaatct cacgccttcc tgtaactctt
     3001 ttggccaatt tgacagccgc ttcgttcgct tcacttcctg agttgacata atagacgcta
     3061 ttcagatttt caggcaaaca agatgtgagc aaagtcgcat gctcaacttg tggagcctgt
     3121 atcaattcac catataccat caaatgcatg taaagttcaa tctgagcctt aactgcatca
     3181 ataacctcct tgttggcatg tcccacattc gagactgata caccggagat aagatcaatt
     3241 atttttcccc catcaggtcc ataaagaaaa actccttcgg ctttttcgat ttcaagacca
     3301 aaagggtaag gggaagtctg cgctatatgc ctgaaaaaaa gctccctgag aatatagggt
     3361 actgaagaca tttactcttt ttcaaagtca gcactaacaa ggattctgcc acagtattca
     3421 caaacaatta tttttttgct tgaagcgata tccatctgcc tttgaggggg tatcttgtta
     3481 aaacatccag cacaagcatc tctcctgact gtaacaaccg caaggccatt tctcgcatta
     3541 catcgaacct tttgataagc agcaagcatc cttgcatcta tctgggaagc aatttcttca
     3601 gatttcttta tcaactcctt ttcttctttg gcagtctcgg taataatgga ttcaagttcc
     3661 tttttcttat tctcaagatc tatggtcctc tccttgatag cttcctgagc agcggcaata
     3721 gcctctttct tttcggctag caacaaagtc ttctctttaa taatcttctc tgcatgctgc
     3781 acttcaagcc cctgatattc tatctctttc gacagagaat catactcacg gttattctta
     3841 acattattct gctgactggt atatttttct atgagattct ttgaattctc aagatccatt
     3901 ttcttctgag caattaattt ctcaaactcc ttgtactctg tctgcaaatt ttccagcctt
     3961 gtcttcaatc cctgaatatc atcctcaaga tcctgaacct ccagtggcaa ttctcctcta
     4021 agaagatgaa tcttatctat cttactgtct gtctgctgca aattgtatag aagacgcaac
     4081 tttgtctcca tactcaggtc accttcattg gcggttttct gcaaagagat gaatgttccc
     4141 ccatccagag gggtttcaat attggatttt gatttagctg ttgccataat ttcttaatag
     4201 tagtatatgg gattaatatt actcgatgca atatgaattg caaatgtagc catttttttt
     4261 aaaagttcgt tctttatcac ttcaagtaca caagactcac tctcaaaatg accgatatcc
     4321 atcaaagcaa agccgggctc tgtaaaaaaa agatggtatg aaagatcggc agttataaaa
     4381 acatctgccc cggatttttt tgcagcctct attaatgatg ctccgcttcc accacaaagt
     4441 gcaactcttg acacaagctt gtcaagaggt ctggaagatt taacacattc tgttccgaat
     4501 actcttttga ctctcagaat tagctcatta aaagatacgg gttctataaa attacccaca
     4561 attccaagtc ctgtatcacc cccaggatac ggatcaagaa atgacaggtt tgacaaacca
     4621 agcttacttg ccatctcgcc actcactcct gcaattacct tatctaaatt agtatgaagt
     4681 gaatatatta caatgtcatt tttaattgcc ctgccaatta ttctttcagt aaaagtggct
     4741 tgagtaatct gcttaatacc tctaaaaatc aaagggtgat gagtaactat catttctgcc
     4801 cccaatttaa cagcctcgtc caaaacctcc tctgtacagt caagagcaag cagtaaagaa
     4861 gtgacttccc tttccgcatt gccgatacaa agtcctgaat tatcccactc ctcctggata
     4921 tctaaaggag ccaaagattc aagaatttct attatccggc ttgcctttgt cataacgatt
     4981 ctgctatttc caataaaata gttttaatgt tattcaatga tgctatcaca tcaacgcgtc
     5041 tatctacgcc tgtcttattg tctttcattc ttttcgcagc tccctcaagg gtcatgcctc
     5101 tctcttttac aagataatga atgatcttga aattggaaag atcctcagca gtaaagagtc
     5161 tgttgccttt tttgtttctt gcgggtttta tatattcagg aaatttttga gcccagaatc
     5221 ttacaagtga agtgttttca ccaagaatct cggcaacttc acctatggtg taaaaaagtt
     5281 tatcgacctt aaattctttg tacggcatga tgattattat gttttcaatt attaatcgag
     5341 agactggcca ctgctcatgg caataatttt catctctctg tactgttctg gagataagtc
     5401 agcaaagaag tagtcaaccg gatccatgac ttcaccattt aaaataacct cataatgcaa
     5461 atgtggagca aaagaaaggc cgctgttgcc tactcttgct ataattgcac cctgctttac
     5521 aggctgcccc tttcgtacaa gaatatctcc aagatgtgca tatttggtaa cgtatccatt
     5581 agagtgagag ataacgagca catttccact ccccttgtct ccagtctttg taatctctga
     5641 aacaactcca tctgcagtag catgtatttg agcacctaat gatgcaatga gatcaagtcc
     5701 agtatggtca acagccgttt tgaaaaatgg atgtattctt ttaccgatac ctgctcctgt
     5761 ctgagatatc tcaattccct ttatgggcag tattgacgga attgatttaa catctggatt
     5821 attcttgatt aagacactga ttgcttctat ctttttattt tgatccacca gcatgctctc
     5881 cacaagttta acctttgagg cgatcatgag caccaactgg tcatcggaag atgagtccaa
     5941 ctgagcataa agaacagacc gattattaac aggaaagatt tctggaggtt ctgacttgaa
     6001 tatactctga taaatatctc tgtctctatt ttcaagattt tgaataacat tatccaaaac
     6061 ctccattttc ttgactgctt ctctatattg ctgagcctta agttcatttt cctttataag
     6121 tctctcttca gcgggagtgt taacaaaaaa ggcaattatt atataataga aaattgcaag
     6181 taaaacagtt aataagagat aacgcaccac tacgcgaatc ttgccagtga cgcccaattt
     6241 atcttcaaca aattttagct gttccttatt gaatctatac ttactcataa caagcctgcc
     6301 aactaacctt ttggagtcac catggcaagg aactcttctg gcttaatatc tctgttatag
     6361 taatttaaag gatttacggg acgcccttga tatataactt cgtaatgaac atgaggtccc
     6421 tttgatctgc ctgtgttgcc catcaacgct atctgctccc cccttcttac ctgtttaccc
     6481 ataactacaa gacttgcctt taaatgtgca tatcttgtct tatatccaaa tccatgatca
     6541 ataataataa aattgccata accgaaaaag tcatagccaa tctcaactac cttgccattt
     6601 ccggtagaat aaataggttc tccaacctgc cctgaaagat ctactcctgt atgcattctg
     6661 tactggccag taaaaggatc aggtctgtaa ccaaaaccac ttgaaaaacg aatccggtga
     6721 ctggaaatat taacaggagg tatagctggc acacttaagg ccagtttatc ggcattttta
     6781 gcctgttcga taacctcatc aaaagacttt gactgcacga aagccttctt ataaagtata
     6841 tcaagatttt cggcagttct ctcaagcaaa ccagaattgt caaaattttt gagataagaa
     6901 tatctgtcaa ctcctccaaa acctgcattt ctaacttcct gaggtatctc ttccatacca
     6961 aaaacaggcc tgtaaatatt attgtccctc atttgtaaag atatcagtga attgtttgcc
     7021 tcttgaaagc gcttattcat gagatccagc ctgtttgaaa gttcctcaga tttactcaac
     7081 aagatcatca tcttgggagt ttcaaaacca aaatactttg tatagagcat ataacaaccc
     7141 gtacttgtaa gaagactcgc caaaaacatg agaaaaccct ttgaaaactt aacgcgcata
     7201 ggaatcttgt gcaactcgta agctaaagtc tctggattaa atttatatct acgttttttt
     7261 ggcatggcat aaagaaaatg gggctaattt tgcgcaaatt taaaaaaaat taaataaaaa
     7321 cctatttttg tggttcattt agattatatt attaatagtg attatgacat caaacgaaat
     7381 cagagataaa tttttaaact tttttgcgag caaggatcac accatattac catcagctcc
     7441 catgattgtc aaagatgacc caactctcat gttcacaaat gcaggtatga accaattcaa
     7501 agactggttt ctaggtaata ccccagcaaa atataaaaga gttgctgact ctcaaaaatg
     7561 tctaagagtc agtggcaagc acaatgatct tgaggaagtt ggccacgaca gctatcatca
     7621 caccatgttt gaaatgctag gaaattggag tttcggagac tatttcaaaa aagaagcaat
     7681 tgagtgggcg tgggaattat tgactgaaat atataaaatc gacaaagagc gtctttacgt
     7741 tacagttttt gaaggaagta aagaagacaa catttctcca gacgaagaag caaaaacaca
     7801 atggcttaag tttttacctg gagatagagt cctgtttggt aacaaaaaag ataatttctg
     7861 ggaaatgggt gataccggac catgtggtcc ttgcagcgag attcatatcg atatacgcag
     7921 tactgaagat agaaaaaaaa taagcggcgc atcccttgta aataaggggg atccacatgt
     7981 tattgaaatc tggaatcttg tatttattca gtataacaga aaagctaatg gagaattagt
     8041 ccaattacct cagaaacacg ttgatacagg aatgggactt gagcgactct gcatgataat
     8101 tcaaggaaaa caaagcaact atgacaccga tctgttcact aatatgattg cctccatatc
     8161 tcgtctaagt ggcaaaagct ataatgaatc agaacaaata tcagtagcaa tgagagtcat
     8221 tgccgatcat atccgagcca taagtttttc aattgctgac gggcaacttc cgtccaatgt
     8281 caaggcagga tatgttatcc ggcgtatcct gcgaagagct gtacgctatg cttatacatt
     8341 tcttggtata gatcagccat tcttgtgtaa actcgtacct actcttgtaa ctaatctcgg
     8401 tgaagcttat cctgagctga tcaaacaaca atcccttatt gagaaagtta tcaaagagga
     8461 agaagaggct ttcctcagaa cattggacaa aggtattaaa ctgatggaga cattaataga
     8521 aaaaaataaa acaaccaaag tcattcctgg aaaagaggca tttactcttt atgacaccta
     8581 tggtttcccc attgacctga gtgaattaat tgcaaaagaa catggctaca caatcgacct
     8641 tagtaccttt gaaaaagagc tctccaaaca aaaagagaga agtcgtaatg cgacctccac
     8701 agaagaaagt gactggatag aacttcgtcc ttttaatggg gtcgaatttg tgggttacga
     8761 caa
//
LOCUS       DAOTQL010000071         8546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7190_length_8546_cov_5.619715, whole genome shotgun sequence.
ACCESSION   DAOTQL010000071 DAOTQL010000000
VERSION     DAOTQL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7190_length_8546_cov_5.619715"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..822)
                     /gene="rplQ"
                     /locus_tag="P5293_05280"
     CDS             complement(301..822)
                     /gene="rplQ"
                     /locus_tag="P5293_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009237641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRR49347.1"
                     /translation="MRHNKTINHLGRKSGHRKAMLANMASSLIIHKRIETTLPKAKAL
                     REYVEPLITKSKDDSTHSRRTVFAYLKQKEAVTELFRVVAPKIADRPGGYTRILKTGF
                     RQGDAADMALIELVDFNEVAVAAAKTETKKATTRRSRAKKSTDVVEASEPAAEKKPKA
                     KAEAKPAAEEKAE"
     gene            complement(809..1822)
                     /locus_tag="P5293_05285"
     CDS             complement(809..1822)
                     /locus_tag="P5293_05285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRR49348.1"
                     /translation="MAILAFQKPDKVIMLDATETFGRFEFRPLEPGYGITIGNALRRI
                     LLSSLEGYAITSVKIHGVDHEFATIPGVIETVVDIILNLKQIRFKQIVDGEDSEIVNI
                     TVSGKGNFIAEDISKKLSSFKVLNPDWHICSMEPSVVLNIELRIGKGRGYVPAEENKV
                     DDAPFGLIAIDSIFTPIKNVKYSVDNWRVEQKTDYEKLNIEITTDGSIHPKEALKEAA
                     KILIHHFMLFSDEKITLETPLEVTSNELDEESLRMRHLLLTKLADMELSVRALNCLKA
                     ADIETLAELVSLQRSELMKFRNFGKKSLSEIDQLIERYRLSFGMDVTKYNIEKKVLKH
                     EAQ"
     gene            complement(1835..2440)
                     /gene="rpsD"
                     /locus_tag="P5293_05290"
     CDS             complement(1835..2440)
                     /gene="rpsD"
                     /locus_tag="P5293_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRR49349.1"
                     /translation="MARYTGPSTKIARKFGEPIFGPDKSFEKKNYPPGQHGLAKKRKK
                     SSEYGMQLKEKQKVKYTYGILERQFRNLYEKASRMKGRKGENLILLLETRLDNLVYRM
                     GIAPSRAAARQLVTHCHIVLDGEVCNIPSTLVKPGQIVGVRERSKSLEVIQNNVSRGA
                     SRFAWIEWDGVSLAGKLISMPERTEVPEVINEQLIVELYSK"
     gene            complement(2471..2863)
                     /gene="rpsK"
                     /locus_tag="P5293_05295"
     CDS             complement(2471..2863)
                     /gene="rpsK"
                     /locus_tag="P5293_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRR49350.1"
                     /translation="MAKKTGTTNKKRVVKVDAYGQLHVSSTFNNVIITLTNSTGQVIS
                     WSSAGKMGFRGSKKNTPYAAQTAAADCAKVAYDLGLRRVKAYVKGPGAGRESAIRTVH
                     SAGIEVTEIIDVTPLPHNGCRPKGRRRV"
     gene            complement(2874..3251)
                     /gene="rpsM"
                     /locus_tag="P5293_05300"
     CDS             complement(2874..3251)
                     /gene="rpsM"
                     /locus_tag="P5293_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRR49351.1"
                     /translation="MARIAGVDLPNNKRGEIGLTYIYGIGRSSARKILSTLGIDFDTK
                     VKDWTDEQIAAIRSMIASDYKIEGELRSVVQMNIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGKKKTVANKKKATK"
     gene            complement(3290..3406)
                     /gene="ykgO"
                     /locus_tag="P5293_05305"
     CDS             complement(3290..3406)
                     /gene="ykgO"
                     /locus_tag="P5293_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRR49352.1"
                     /translation="MKVKTSIKKRSEDCKIVKRKGRLYVICKKNPRFKMRQG"
     gene            complement(3418..3636)
                     /gene="infA"
                     /locus_tag="P5293_05310"
     CDS             complement(3418..3636)
                     /gene="infA"
                     /locus_tag="P5293_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRR49353.1"
                     /translation="MAKQPAIEKEGTIIEALSNAMFRVELDNGHIIIAHISGKMRMHY
                     IRILPGDRVKVEMSPYDLTKGRISFRYK"
     gene            complement(3657..4424)
                     /gene="map"
                     /locus_tag="P5293_05315"
     CDS             complement(3657..4424)
                     /gene="map"
                     /locus_tag="P5293_05315"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRR49354.1"
                     /translation="MVKLKTEEEIELLRENALLVSRTLAEVGKHIVPGITTLELNNIA
                     ETFIRDHGAVPAFLGYNGFPYSLCISVNDEVVHGFASDYRLKEGDIVSVDCGTVMKGY
                     FGDSAYTFPVGDVSPETFKLLEVTKESLYRGASKAVVGNRIGDISFTIQSYVESFGFS
                     AVREMVGHGIGKRLHEAPEVPNYGRVGDGKKLIEGMVICIEPMINAGTKSIIMHDNGW
                     TVSTLDKKNSAHFELMVAVRGGKPDILSTFKFIEEKN"
     assembly_gap    4554..4653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4654..5927)
                     /gene="secY"
                     /locus_tag="P5293_05320"
     CDS             complement(<4654..5927)
                     /gene="secY"
                     /locus_tag="P5293_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRR49355.1"
                     /translation="MKKFIETIKNIFKIEELRKRIVYTILLLLVYRLGSFVVLPGIDP
                     TQLDKLQSQASEGLLALLNMFSGGAFGNASIFALGVMPYISASIVIQLLGVMVPYFQK
                     LQREGESGRRKMNQWTRYLTIVILILQGPAYMANLHAQLPESAFLFHGFSYNIFATIV
                     LIGGTMFIMWLGERITDRGLGNGISLIIMVGIVARLPFALVSEAGARLSQSAGGGILM
                     LIVELVILFFVFVATIALVQGTRKIPVQYAKRIIGNKQYGGVRQYIPLKVNAAGVMPI
                     IFAQAIMMFPLLLSGFNATRGLAATLSNSTGFWYNLLYGLMVIAFTYFYTAVTVNPAN
                     MAEDMKKNGGFIPGIKPGKKTAEYLDSIMSRITLPGSFFLAIVAILPAFAKMLGISNQ
                     FASFFGGTTLLILVGVILDTLQQIESHLLMRH"
     gene            complement(5936..6382)
                     /gene="rplO"
                     /locus_tag="P5293_05325"
     CDS             complement(5936..6382)
                     /gene="rplO"
                     /locus_tag="P5293_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRR49356.1"
                     /translation="MKLHNLKPAEGSTGRVKRVGRGEGSGHGGTSTRGMNGAKSRSGY
                     SRKLGFEGGQMPLQRRLPKFGFNNINKVEYKAINLFTLQALSDKSGITTFNIETLIDA
                     GLISKNDKVKILGNGELTAKLDVTAHAFSQSALAKIEAQQGKATKI"
     gene            complement(6398..6580)
                     /gene="rpmD"
                     /locus_tag="P5293_05330"
     CDS             complement(6398..6580)
                     /gene="rpmD"
                     /locus_tag="P5293_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRR49357.1"
                     /translation="MTKVRVTQVRSKNSANKRQIATLTSLGIHRIGHSVELELNPVNK
                     GMIGKVLHLVKVEEIN"
     gene            complement(6593..7111)
                     /gene="rpsE"
                     /locus_tag="P5293_05335"
     CDS             complement(6593..7111)
                     /gene="rpsE"
                     /locus_tag="P5293_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRR49358.1"
                     /translation="MANTNIKKVKTTDLELKDRLVAVQRVTKVTKGGRHFSFAAIVVV
                     GDEKGVVGYGLGKANEVTTAISKGIEDAKKNLVKIPLNKKTIPHEQEAKFGGARVFMK
                     PAAEGTGVKAGGAMRAVFESVGVHDVLAKSKGSSNPHNLVKATVAALLEMRDAYTIAG
                     VRGIPMNKVFKG"
     gene            complement(7118..7480)
                     /gene="rplR"
                     /locus_tag="P5293_05340"
     CDS             complement(7118..7480)
                     /gene="rplR"
                     /locus_tag="P5293_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRR49359.1"
                     /translation="MALSKIERRKRIQHRIRKVVNGTPERPRLAVFRSNKQIYVQFIN
                     DIDGVTITSASSRDKEIVEECKGKPGIEVAKVVGKLAARRAIEKGISEVSFDRGGYLY
                     HGRVKSLADAAREGGLRF"
     gene            complement(7495..8055)
                     /gene="rplF"
                     /locus_tag="P5293_05345"
     CDS             complement(7495..8055)
                     /gene="rplF"
                     /locus_tag="P5293_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRR49360.1"
                     /translation="MSRIGKLPVNLPAGVTVKVEPGNIVKVKGPLGELTQKVDPDITV
                     TVAGNVVTVSRPTDQPRHRSLHGLYRALINNMVVGVSTGYTVKQEFVGVGYRAEVKGQ
                     VLELSLGYSHDIHFLLPKEIKASAEVAKKGANPVLTMTSHDKQLLGTVAAKIRSLRKP
                     EPYKGKGIKFVGEQLRRKAGKSASAK"
     gene            complement(8081..8476)
                     /gene="rpsH"
                     /locus_tag="P5293_05350"
     CDS             complement(8081..8476)
                     /gene="rpsH"
                     /locus_tag="P5293_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRR49361.1"
                     /translation="MTDPISDYLTRLRNAYLAGHKIVEIPASKMKLEITRILHEKGYI
                     LSYKIVEGKPYNTIKIALKYHPDTKKAAIKKITRVSSPGLRKYVSVANLPRVLNGLGI
                     AIISTSKGVITDKEAKEMNIGGEVICYVY"
BASE COUNT         2373 a         1942 c         1407 g         2724 t
ORIGIN      
        1 agaaagaatt ttattaatca tgtcctcgac acctttagta acaaaaatac tgttcttagg
       61 tatcatgtat gcattacgat taaaactatc agacattctg cttataacac ataatacaca
      121 actgaatgtt caggcacatt aaggaatagc taatccaata cagccattaa tacatcattc
      181 aaacaacact ggagttagat atggtgtcat attgcaaaag gaaagagtcc ggtgcaatac
      241 cgaactcttg cataataaat ttttaaacga gcgctcttct ttagagtttg agttatgcaa
      301 ttactcagct ttctcttctg ctgcaggctt agcctcagct tttgcctttg gtttcttctc
      361 tgcagcaggc tcagaagctt caacaacatc agtgctcttc tttgcacggc tacgacgtgt
      421 agttgccttc ttggtctcag tttttgcagc tgcaacagct acctcgttga aatcaactaa
      481 ctcgataaga gccatgtcag cagcgtctcc ctgacggaaa cctgtcttaa ggattcttgt
      541 gtatccaccc ggacgatcag caatttttgg tgcaacaaca cgaaaaagct ctgttacagc
      601 ttctttttgc ttcaaataag caaaaactgt acgccgagaa tgagtcgaat catcttttga
      661 ttttgttatt aaaggctcca catactctct cagtgccttt gccttgggta atgttgtttc
      721 aatcctcttg tgaattataa gagatgatgc catattggca agcattgcct tccggtgccc
      781 gctcttacga cccaaatggt taattgtctt attgtgcctc atgttttaaa actttctttt
      841 caatattata ctttgttaca tccatgccaa aactaagtct gtagcgctca ataagctgat
      901 ctatctctga aagcgatttc ttgccaaagt ttctaaactt cattagttct gacctctgaa
      961 gagataccag ttccgcaaga gtttcaatat cggctgcctt aaggcaattc aaagccctaa
     1021 cagaaagctc catgtcggca agttttgtca aaagaagatg acgcattctg agagactctt
     1081 catcgagctc attgcttgtg acctctaagg gagtttcaag tgttatcttt tcgtctgaaa
     1141 acagcatgaa gtggtgaata aggatttttg cagcctcctt cagagcttct ttcggatgaa
     1201 ttgaaccgtc tgttgtaatc tctatattga gcttttcata gtcagttttc tgctctacac
     1261 gccagttatc aactgaatac ttgacattct ttattggagt gaaaatagaa tcaatcgcga
     1321 taagcccgaa aggggcatca tcaaccttgt tttcctcagc agggacataa cctctcccct
     1381 tgcctatcct cagctcaata ttaagtacaa cagagggttc catggaacat atatgccaat
     1441 ctggattcaa aactttgaaa gaagataatt tttttgaaat atcttcagca ataaagtttc
     1501 ctttgccact tactgtaata tttacaatct ctgagtcctc tccatctaca atttgtttaa
     1561 aacgaatttg ctttaaattc agtattatat ctacaactgt ttcaattact ccaggaattg
     1621 tggcaaattc atgatcaact ccatgaatct tgaccgaagt gattgcatac ccttccaaag
     1681 atgagagaag aattcggcgt agtgcgttcc ctattgtgat accataacca ggttccagcg
     1741 gacgaaactc aaaacgacca aacgtctcag ttgcatcaag cattatcacc ttatcgggtt
     1801 tttgaaatgc taatattgcc attttattta ttatttattt agagtataac tcaactatca
     1861 gttgttcatt aattacttca ggcacttctg ttctctcagg catacttata agcttaccgg
     1921 caagagatac tccatcccac tctatccagg caaacctgct tgcaccacgg gaaacattat
     1981 tctgaataac ttccaaactt ttagatctct ctcttacccc gacaatctgg ccaggcttaa
     2041 caagtgtaga aggaatattg caaacttccc catcaagcac aatgtgacaa tgtgtaacaa
     2101 gttgtcttgc tgctgccctg gaaggagcaa tacccattct gtaaaccaaa ttgtcgagac
     2161 gggtctcaag aagtaaaatc aaattctcac cctttcttcc tttcattctt gaagcctttt
     2221 cgtacagatt gcgaaactgt ctctcaagaa taccgtaagt atatttaact ttctgctttt
     2281 cctttaactg catcccatat tctgaagact tcttgcgctt ttttgcaaga ccatgttgtc
     2341 cgggagggta atttttcttt tcaaagcttt tatccggacc aaatataggt tctccaaatt
     2401 ttcgggcaat ttttgttgac ggccctgtat atcttgccat attagataat tttaaattaa
     2461 ttattaaaga ttaaactcta cggcgccctt ttggacgaca tccattatgt ggaagcggag
     2521 tcacatcaat tatttcagtt acttcaatcc ctgcactgtg tacagtacgt atcgcagatt
     2581 cacgtcctgc tcccggacct ttcacataag ccttcaccct acggagaccc aaatcatatg
     2641 caacttttgc acaatcggca gctgctgtct gagctgcgta aggagtattc tttttagaac
     2701 ctctgaaacc catcttacct gcagatgacc aactgattac ctgacctgta gagttagtta
     2761 aagtaattat cacattattg aaagtagatg aaacatggag ttgcccataa gcatccacct
     2821 ttacaactct tttcttgttt gttgttcctg ttttctttgc catgacctaa cctctattta
     2881 gttgcttttt tcttgttagc tactgttttc ttcttaccct tgcgtgttcg ggcattgttt
     2941 ttagttgact gtcctcttac aggcaaacct aaacgatgac ggatacctct ataacatcct
     3001 atatccatca acctcttaat gttcatttga actactgagc gcaactctcc ctcaattttg
     3061 taatccgatg caatcatgga tctgattgca gcaatttgct catctgtcca gtcctttact
     3121 tttgtatcaa aatcaatccc cagtgtagag agaattttgc gtgcagaact gcgaccgata
     3181 ccgtagatat aggtaagacc tatctctccg cgtttattgt ttggtaaatc tactccggct
     3241 atacgtgcca tatctgtttt ttctctttat tgttttacaa attaataaat tacccttgac
     3301 gcattttgaa ccttgggttt ttcttgcaaa taacatacaa acgacctttg cgttttacaa
     3361 tcttgcaatc ctcactgcgt ttctttatgg atgttttaac tttcattttt atatttttta
     3421 tttgtatctg aaagaaattc ttcctttggt taaatcatat ggggacatct ctaccttgac
     3481 tctgtcaccg ggaagaatac gaatataatg cattctcatc ttgccggata tatgggcaat
     3541 aatgatatgt ccattatcca actctacacg aaacattgca tttgaaaggg cctctataat
     3601 tgttccctct ttttctattg ccggttgttt tgccattaaa gtttttaacc cctgttttaa
     3661 tttttctctt ctataaattt aaatgtagat agaatatcag gttttccacc ccttactgcc
     3721 accattaatt cgaagtgtgc agaattcttt ttgtcaagtg tactgacagt ccatccatta
     3781 tcatgcatta taatgctttt ggtacctgca ttgatcatcg gttcaataca aataaccatt
     3841 ccttctataa gtttcttacc atctcccact ctcccgtaat tcggaacttc aggagcttcg
     3901 tgaagccttt ttccgattcc gtgccctacc atctctctga cagctgaaaa tccaaaactt
     3961 tccacataag actggatcgt aaatgagata tcacctattc tattgccaac aactgctttg
     4021 ctcgcacctc tgtaaagcga ctcttttgtt acctcgagaa gcttgaaggt ctcaggcgaa
     4081 acatcaccaa caggaaatgt atatgcggaa tcaccaaagt accctttcat cacagtacca
     4141 caatcaactg aaacaatatc accctccttg agacgatagt ctgaagcaaa accatgtaca
     4201 acctcgtcat tgactgaaat acaaagggaa taagggaacc cgttataacc caggaaagct
     4261 ggtaccgcac catgatctct gataaaggtt tcggcgatat tgttaagctc cagggttgtt
     4321 atcccgggaa ctatatgttt gccaacctct gccaaagttc tggataccag tagagcattt
     4381 tctctaagta actctatctc ctcctcggtt ttaagtttaa ccatatctac aaagaacttt
     4441 tgaatcctgt tacatccctg aacgtccctt taaacggcct gttttcataa gaccatcata
     4501 gtgacgcata agtagatgac tttctatttg ttgcaaggta tcaagaatta cacnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntagtgac gcataagtag atgactttct
     4681 atttgttgca aggtatcaag aattacacct acaaggatga gcaatgttgt gcccccaaag
     4741 aatgatgcaa attggttact gatacccagc atttttgcaa atgcaggcaa aatagctact
     4801 atggcaagga aaaacgagcc tggcagcgta atgcgagaca ttattgaatc aagatactct
     4861 gcggttttct ttcccggttt aataccagga ataaaaccgc catttttttt catatcctca
     4921 gccatattgg caggatttac cgttaccgca gtatagaagt aggtaaatgc tataaccatg
     4981 aggccataca gcaagttata ccaaaagcct gttgaatttg acagtgttgc tgccaaacct
     5041 cgagtggcat taaaacctga cagaagcaag gggaacatca taattgcctg tgcaaagata
     5101 atcggcatga cgccagccgc atttaccttc aaaggtatat attgacgaac gcctccatat
     5161 tgcttattac caattatcct ttttgcatac tgtaccggaa tcttgcgtgt cccctgaaca
     5221 agagcaatag tggcaacaaa tacaaaaaac aaaatcacca gctcgacaat caacattaaa
     5281 attccaccgc ctgcgctttg actaagtctt gcacctgctt cagacacgag tgcaaatggt
     5341 aagcgtgcta caatacccac cataatgatt agtgaaatac cattacctaa gccgcgatcg
     5401 gtaattcgtt ctcccagcca cattatgaac attgttccgc ctatcaggac aattgttgca
     5461 aatatattat atgaaaatcc atgaaaaaga aaagctgact caggcaactg tgcgtgaaga
     5521 ttggccatat aagctggacc ttgtaatata agtatcacaa ttgttaaata acgtgtccac
     5581 tgattcatct ttcttctccc gctctcacct tcacgctgta gtttttggaa ataaggcacc
     5641 ataacgccaa gcaattgtat cacaattgaa gcagaaatat atggcatcac tcccaaagca
     5701 aaaattgatg cattaccgaa ggcaccaccg gaaaacatgt taagcaaagc taacaatcct
     5761 tcagatgctt gtgactgcaa tttatcaagt tgagtaggat cgattccagg taatactaca
     5821 aaacttccaa gacgataaac gagcaacaaa agaattgtgt acacaatcct tttgcgcagt
     5881 tcttcaatct tgaagatgtt tttaattgtt tcgataaatt ttttcatcag aaaatttaga
     5941 ttttagttgc ctttccctgt tgtgcttcga tctttgccaa tgcagactga gaaaatgcgt
     6001 gagctgttac atccagtttt gctgtaagtt ctccattacc aagaatttta actttgtcat
     6061 tcttggaaat taaacccgca tcaataagag tctcaatatt aaaagttgtt attccactct
     6121 tatcacttaa tgcttgcaaa gtaaacaagt taatggcctt atattcaact ttgttaatgt
     6181 tgttaaatcc aaattttgga agtcttcttt gaagaggcat ctggccaccc tcaaaaccca
     6241 acttgcgaga atatcctgaa cgtgatttgg caccattcat tccacgggta gatgtaccac
     6301 catgtccgga accttctcca cgtccaactc ttttaactct tccggtagaa ccctcagcag
     6361 gtttaagatt atgtaatttc attgtaaaat ctcctgatta attaatttct tcaactttca
     6421 ccagatgcaa taccttgcca atcatcccct tgtttacagg gttcagctcc agttcaacag
     6481 aatgcccaat tctgtgaatt cccagagaag tcagtgttgc tatttgtctc ttatttgcac
     6541 tatttttact ccttacctga gtaactctaa ctttagtcat aatcttgcct ccctatcctt
     6601 taaacacttt attcataggt atacctctaa cgcctgcaat agtataagca tcgcgcattt
     6661 caagaagggc agcaacagtc gctttcacca aattatgagg atttgacgat cctttagact
     6721 tggcaagaac gtcatgaact cctactgact caaagacagc acgcatagca cctccggcct
     6781 ttactcctgt tccttcagct gcaggcttca taaaaactct cgctcctcca aatttggctt
     6841 cctgttcatg agggattgtc ttcttattaa gaggaatctt aacaagattt ttctttgcat
     6901 cttctatccc ttttgaaatg gcagttgtaa cctcattagc cttacccaga ccataaccta
     6961 caactccttt ttcatctccg accaccacaa ttgcagcgaa actgaagtgg cgacctccct
     7021 ttgtaacttt ggtaactctt tgaactgcca ccaatctgtc cttcaactca agatcggtag
     7081 tcttaacttt tttgatgttt gtatttgcca tactatacta gaatctaaga ccaccttctc
     7141 tggcggcatc tgctaaactt ttaactcttc cgtgatatag ataacctcct cgatcgaagg
     7201 atacctctga aattcctttt tcaatagcac gtcttgctgc aagctttcct acaacctttg
     7261 caacttcgat gcccggtttg cccttgcact cctcaacaat ctccttgtcc cttgaactgg
     7321 cagatgtaat tgttacgcca tcaatatcat tgataaactg aacataaatc tgcttgttac
     7381 ttctgaaaac agcaagtctt ggtctctctg gagtaccgtt tacaacctta cgtattcggt
     7441 gctgaattct ctttcttctt tctattttac ttaatgccat aaatcctcca cttgttattt
     7501 agcgcttgcc gacttaccgg ctttgcgacg aagttgttct ccaacaaact taataccttt
     7561 gcctttataa ggttccggtt tcctcaacga tcttatcttt gcagccacag ttccaagaag
     7621 ttgtttgtcg tgactggtca ttgtcagtac tggattggct cctttttttg ccacttccgc
     7681 agaagcctta atctcttttg gcagtaaaaa atgaatgtca tgtgaatacc caagactgag
     7741 ctctagaact tgtcccttta cctctgccct gtaacccaca ccaacaaact cctgcttaac
     7801 tgtataacct gtagaaactc ctacaaccat attattaatc aaagccctgt aaagtccatg
     7861 caatgaacgg tgacgaggct gatcagttgg tctactgact gttactacat tccccgctac
     7921 agtaactgtg atatcaggat cgactttctg tgtaagttct ccgagcgggc ctttaacctt
     7981 tacaatattt cccggttcca ccttgacggt tacgccggct ggaaggttta caggtaattt
     8041 tcctattcgt gacatttgtt atttattatt aaattcataa ctaatataca tagcagataa
     8101 cctctccgcc tatgttcatt tcttttgcct ccttgtcggt tattacgccc tttgaagtag
     8161 atataatggc aattcccagc ccattaagta ctcttggcaa attggccaca ctgacatatt
     8221 ttctaagacc aggagaacta accctggtaa tcttttttat ggcagccttt ttagtatcag
     8281 gatgatattt aagagcaatc ttgatagtat tatatggctt gccctcaact atcttatagc
     8341 tgagaatata acccttttca tgaagtatgc gggttatttc cagcttcatc ttggaagcgg
     8401 gaatctcaac aattttgtga cctgcaagat atgcattacg caatcttgtc aaataatctg
     8461 aaattggatc agtcattgtt ttgtttgttt taaagtaccg acatccaaaa aaaggatgcc
     8521 cgatatccaa tgttatttaa aatttt
//
LOCUS       DAOTQL010000072         8523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7209_length_8523_cov_5.241615, whole genome shotgun sequence.
ACCESSION   DAOTQL010000072 DAOTQL010000000
VERSION     DAOTQL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_8523_cov_5.241615"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..491
                     /locus_tag="P5293_05355"
     CDS             54..491
                     /locus_tag="P5293_05355"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRR49362.1"
                     /translation="MAASKFLSDSDKSLLIKEIESAELNTSGEIRVHIESKCNSDPIS
                     RAITVFNALKMYRTKERNAVLIYIAYESKKLAIIGDAGINSTVPEGFWDDVKDVLVSH
                     FASNQIVDGLRIAIRMTGDKLRDYFPHKTDDTNEQSNEISFGE"
     gene            469..1293
                     /locus_tag="P5293_05360"
     CDS             469..1293
                     /locus_tag="P5293_05360"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRR49363.1"
                     /translation="MKYRLVNKPLTLLVLLTIAVTSVLAQVPKRPSPPRLVNDFAVIL
                     TPEQSTYLEDTLVNFANKTSNQIVIVTLNDFGGMERSQMAYSIGEEWRVGQEKFDNGI
                     VILIKPKRHDERGEAFIATGYGLEGALPDAICKRIVEQEMIPHFMLGEYYEGIVEALK
                     VIMPIAAGEFSSEEYAAQSEGSSLFGALIVIGIVLIFIIFSIVNKRKGPTNFGGGNRK
                     DPGFLDLLILGSLLSGRGGRFGGYSGGGLGRGGFGGGGFGGFGGGSFGGGGAGGSW"
     gene            complement(1296..2438)
                     /locus_tag="P5293_05365"
     CDS             complement(1296..2438)
                     /locus_tag="P5293_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR49364.1"
                     /translation="MKIVFFLIAFSVISLIAAYPFVRGLQAFSYSRWLSVIYATIYIF
                     AFLAFMLKMFIGETTNSSISLFLSRVGFTWFIAVIYFSLFALSFDLLRLINHFTGFFP
                     SFIKDNYQLVKLLLAVFVGLAVSSLLIYGNWKFNHPVVRELSIKIDKPLPPDGVNIVL
                     ASDIHLSTIINRDKLTQYVALINRQNPDLVLITGDISDRNIKPLIDGKLSELLGSITS
                     TYGTYAVTGNHEYYGGDKEALYSYYRSSGIHLLIDSVVVVNVGDGILNIIGRDDKTNH
                     HRKSLGMLIEGVDKKWPVILMDHQPYNLQEAELAGVDLQVSGHTHQGQFWPGSFFVKK
                     MYELSYGYKVRGKTHFIVSSGLGLWGPEFRIGTSSEIVLIHLFSKS"
     gene            complement(2454..4070)
                     /locus_tag="P5293_05370"
     CDS             complement(2454..4070)
                     /locus_tag="P5293_05370"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRR49365.1"
                     /translation="MNFKFQEIFPLGNDKTHYHLLTKKYISVASFEGHEILKIDLEGI
                     RLLVRQAFHDVSFMLRPEHNEQVARILNDPEASANDKYVALTMLLNADVSSKGVLPFC
                     QDTGTAIVIAKKGQKIWTGGGDEEAISHGVFDTYTQDNLRYSQNAPLDMYNEKNTGCN
                     LPAQIDIYATDNDEYDFLCIAKGGGSANKTFLFQETRALLNPDTLEKFLVEKMKTLGT
                     SACPPYHIAFVIGGTSAESCLKTVKLASAKYLDALPTKGNELGHAFRDVELESKLLQA
                     AQKLGIGAQFGGKYFAHDIRVIRLPRHGASCPVGLGVSCSADRNIKGKINKEGIWLET
                     LDADPIRLVPEPLRSGKAAYSEGIKIDLNRPMKEILADLSQYPVSTLLLLNGTIIVAR
                     DMAHAKLKERLDAGEDLPQYIKEHPVYYAGPAKTPKGVPSGSFGPTTAGRMDSYVDLF
                     QEHGGSLIMIAKGNRSQQVTDACKKYGGFYLGSIGGPAAILAKDSIKKVELIEYPELG
                     MEAIWKIEVENFPAFILVDDKGNDFFAQYK"
     gene            complement(4105..5191)
                     /gene="prfB"
                     /locus_tag="P5293_05375"
     CDS             complement(join(4105..5127,5129..5191))
                     /gene="prfB"
                     /locus_tag="P5293_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132894.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRR49366.1"
                     /translation="MVNLEQFKELQQRTSALRRSLDIDGKREKIKELEDRSSSPDFWD
                     DPKSAESFLKNVSSIKYWTDKFASIETSLEDFEILLDLVREDPELEEEVDKAFSQTLR
                     LIEELEMRKMLGEEGDNLGAILKINSGAGGTESNDWSSMLMRMYARWGERNNYKVHIY
                     DLIEGDEAGIKSVTLEFEGEYAYGYLKAETGVHRLVRISPFNAQGKRQTTFSSVFVYP
                     AVDDTIEIEINPGDLEWDTFRSSGAGGQNVNKVETGVRVRHIPTGISVENTETRSQLD
                     NRQNALRILKSHLYELELRKRREKQAELEGSKMKIEWGSQIRSYVLHPYKMVKDLRTG
                     YETSDVQAVLDGDLNDFMKAFLLGTFK"
     gene            complement(5208..5750)
                     /locus_tag="P5293_05380"
     CDS             complement(5208..5750)
                     /locus_tag="P5293_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRR49367.1"
                     /translation="MSLQEGGIQKGESAAKKRYDNHSLLAQMQRLCAIREYTIQDINS
                     KLRNKGCSEHEILEIVGKLIEDNYINEERYAFAFVRDKSTLAGWGRKKIEYALKNKGI
                     GQTVIKIALDSISSESDIALLEKLLLKKINELKPKLESKDDGTKKLREKLLRFALSRG
                     FSYEDILKVINKTLANLEPE"
     gene            complement(5755..>8523)
                     /locus_tag="P5293_05385"
     CDS             complement(5755..>8523)
                     /locus_tag="P5293_05385"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HRR49368.1"
                     /translation="GSNVLTPPKRDPLWKLFLLKFNDPIIRILLVAAFLSLGISFVHN
                     EFVETIGIFCAIFLATGVSFWFEMDASKKFDILNQVNDETLVKVIRDGNIREVPKKDI
                     VVGDIILLDTGEEVPADATLIEAVSLSVNESTLTGEPVIEKSVDPAMFDDEATYPTNN
                     IYRGTTIVDGHCIARVFAVGDSTEYGKVAQKSAEKNPEETPLNRQLDKLAKFVGFAGF
                     VLAVMTFAMLFVKDMFLGNVNYSFSEIFLLVSVITGVVIAITKIWIPAIYDGLSVLGA
                     KVTTPALVRESDWSRWVTFGISTTVILMALGYLIFGVDPSDANQWISIDTASRILNYF
                     MVSVTLIVVAMPEGLPMSVTLSLAMSMKRMLQTNNLVRKMHATETMGATTVICTDKTG
                     TLTQNQMRVAFTNFVVGNDEPIIMESIAVNSTAFLEISDDGKIKALGNPTEAALLLWL
                     HDRGINYLEVRNSAKIIDQLTFSTERKYMATLAESASLGRRILFVKGAPEIVASKCIS
                     LPDGLNDLLLKYQNQAMRTLAFAYKEIPAEACNEKMEVLSERGLTYLGMVAIADPVRP
                     DVPDAVAKCLKAGIDVKIVTGDTPRTAWEIGRQIGIINDNDGPEVIITGVDFEALTDE
                     EALDRVMGLKIMCRARPIDKQRLVQLLQKKGAVVAVTGDGTNDAPALNYAHVGLSMGS
                     GTSVAKEASDITLLDDSFNSIATAVMWGRSLYQNIQRFILFQLTINFAALLIVFIGSL
                     IGKDLPLTVTQMLWVNLIMDTFAAGALASLPPDERVMERKPRKADDFIITKKMAVIII
                     TSGVIFVVFMLGLLEYFTNDAGEISRYDSSRFFTFFVMLQFWNMFNAKRFGSSESAFR
                     DITKSKGFLLVGLLIVAGQVLIVQFGGDVFRTEPISLRDWVLIILITSLVLIVGEAAA
                     IIKRIIKK"
BASE COUNT         2666 a         1842 c         1467 g         2548 t
ORIGIN      
        1 tatttcgaag caaatgaggg gacagaacaa ccaccaaaag ttgaatttta attgtggctg
       61 cctcaaaatt tctttcagat tcagataagt ccttattgat taaggaaata gaatctgcgg
      121 aacttaatac ttccggtgag ataagggtac atattgaaag caaatgtaat tcagatccaa
      181 tcagcagggc aattactgta tttaatgctt taaaaatgta ccgaacaaag gagcgaaacg
      241 ctgtcctgat atatattgca tatgaatcaa agaaacttgc aataattgga gatgcaggga
      301 tcaattcaac agtcccggaa gggttctggg atgatgttaa agatgttctt gtctctcatt
      361 ttgcatccaa tcaaatagtt gatggactta gaattgctat aagaatgaca ggagataaat
      421 taagagatta ctttccccat aaaacagacg acactaatga acagagcaat gaaatatcgt
      481 ttggtgaata agccactgac tcttctggtc ctattgacta tagcagtaac ttcagtactc
      541 gcccaggttc ctaaaaggcc atctcctccg agacttgtga atgatttcgc tgtcatcctt
      601 acaccagagc aaagtacata tctcgaagat actctggtaa attttgcaaa caaaacctcc
      661 aaccagattg tcatcgtgac gttaaacgat ttcggaggca tggaaagatc acagatggcc
      721 tattcaattg gtgaagagtg gagagtcgga caggaaaaat ttgataatgg tatcgttata
      781 cttataaaac ctaaaagaca tgacgaacgt ggtgaagctt ttatagctac tggttatggc
      841 cttgaaggag ctctccctga tgccatatgc aaaaggattg tggaacagga gatgattcca
      901 cattttatgc tgggagagta ctatgagggg attgtcgagg ctcttaaagt gattatgcca
      961 atagctgcag gtgaattcag ttccgaagaa tatgctgcac aaagcgaggg aagttctttg
     1021 ttcggtgcgt tgatcgtcat cggaattgta ctgatcttta taattttttc cattgttaac
     1081 aaaagaaagg gaccaactaa ttttggaggg ggtaatagaa aagacccagg ttttcttgat
     1141 ttgttaatac ttgggtcttt actttctgga cgaggaggac ggtttggcgg atattctggc
     1201 ggaggattag gtagaggcgg atttggagga ggtggattcg gaggattcgg aggcggtagt
     1261 ttcggaggag gtggcgcagg tggcagctgg taaaatcaac tcttagaaaa aaggtgaatt
     1321 aagacaattt ccgacgaagt cccaatacga aattctggtc cccacaaacc aaggcctgaa
     1381 gagacaatga aatgtgtctt tcctctgact ttatacccat atgataattc atacatcttt
     1441 ttcacaaaaa aacttccagg ccaaaactgc ccttgatgag tgtgacctga aacttgcaga
     1501 tctacacctg ccaattctgc ttcctgaaga ttgtagggct gatgatccat taaaattacc
     1561 ggccattttt tgtcaacacc ttcgatcaac atgcccagac tttttctgtg atggttggtt
     1621 ttgtcatctc ttcctataat attcaaaata ccatcaccaa cattaacaac aactaccgaa
     1681 tctatcagca gatgtattcc cgaagatctg taatatgaat aaagtgcttc tttatcacca
     1741 ccataatatt catgattgcc cgtaactgca tatgtaccat aagttgaagt aatactacct
     1801 aataactcag acaactttcc atctatcagt ggctttatgt tcctgtcgga aatatctcct
     1861 gtaattaaaa ccaaatccgg attttgcctg tttatcagag ccacatattg ggtcagttta
     1921 tctctgttga taatggtact taaatgaata tcgcttgcaa gcacaatatt tactccgtct
     1981 ggcggaagag gtttatcaat ctttattgat agctctctaa ccacaggatg attaaatttc
     2041 caattgccat aaattaatag agatgagaca gctaatccaa caaaaacagc caatagaagt
     2101 ttaacaagct gataattatc tttaataaaa gatgggaaaa atccagtaaa atggttgatc
     2161 aacctaagca aatcaaatga aagtgcaaaa agagaaaaat agataacagc tataaaccag
     2221 gtaaatccaa cccttgaaag aaaaagtgaa atcgaagagt tcgttgtctc tccgataaac
     2281 atttttaaca taaaagccaa gaaggcaaaa atataaatag tggcatatat gacacttagc
     2341 cagcgtgaat atgaaaaagc ctgcaaccct ctaacaaacg gataagcagc gattaatgat
     2401 attaccgaaa aagcgataag gaaaaaaaca attttcaatt taaatgattt aatttattta
     2461 tactgtgcaa aaaaatcgtt ccctttatca tcaacaagaa taaaagcagg aaagttctca
     2521 acttctattt tccaaattgc ctccataccc aactctggat attcaataag ttcaactttt
     2581 ttaatactgt ctttagccag gatggctgca ggacctccta tactgccaag atagaaccca
     2641 ccgtattttt tgcaggcatc tgtaacttgc tggctcctgt tacccttagc tatcataatt
     2701 aggcttcctc catgttcctg gaaaagatca acatacgaat ccattctgcc ggcagttgta
     2761 ggtccaaatg agccacttgg cactcctttt ggcgtttttg caggtccggc ataatataca
     2821 ggatgttctt taatatattg tggtaaatcc tcaccggcat ctaatctctc ttttaactta
     2881 gcatgagcca tatccctggc gactataatg gtaccattaa gcaacaagag agttgacaca
     2941 ggatattgtg aaaggtcggc aagtatttct ttcattgggc gattaagatc tatctttatc
     3001 ccctctgaat aagcagcttt accggatctc aagggctcag gaacaagtct gatgggatca
     3061 gcatccaaag tctcaagcca aatgccctct ttgtttatct ttcccttaat atttctgtca
     3121 gcactgcatg aaactcccaa tccgacagga catgaggcac cgtgacgtgg aagacgtatc
     3181 acccttatat cgtgtgcaaa atatttgcct ccgaattgtg ctcctatacc aagtttttgt
     3241 gcagcctgta gtaattttga ttcaagctca acatctctaa aagcatgtcc caactcgttg
     3301 ccttttgtag gaagtgcatc aagatatttg gcagaagcaa gtttcacagt tttaagacaa
     3361 ctctctgcag aagtcccacc aatcacaaaa gcaatatgat atggagggca ggctgaggta
     3421 ccaagagttt tcattttctc cactaaaaat ttctcgagag tatcaggatt gagcaaagct
     3481 ctggtttcct gaaataaaaa agtcttattg gcagaaccac cccctttagc tatgcaaaga
     3541 aaatcatatt catcattatc ggttgcataa atatctatct gggcaggaag attacatcct
     3601 gtattttttt cgttatacat gtcgagagga gcattttgtg aatatctcaa attatcctga
     3661 gtataagtat caaaaactcc atgcgatatt gcctcttcgt ctccacctcc tgtccagatc
     3721 ttttgtccct tctttgcaat tacaattgca gtacctgtat cctgacaaaa aggaagaaca
     3781 ccttttgaag agacatctgc atttagtaac atggtaagag ctacatactt gtcgttcgca
     3841 ctggcttcag gatcattcaa aatcctggcg acctgttcat tatgttcagg ccttagcata
     3901 aaagatacat catggaaagc ctgtctgaca agcagtctta ttccttcaag atctatctta
     3961 agtatttcat gaccctcaaa acttgcaaca gagatatatt tttttgtaag aaggtgatag
     4021 tgtgttttat cgttccccaa tggaaagatt tcctgaaact taaaattcat atttctataa
     4081 ttatataaca attcatatta tctgtcattt gaatgttcct aacaaaaatg ctttcataaa
     4141 atcattcaag tctccatcca acactgcctg cacatcagaa gtttcatacc ctgtccttaa
     4201 atctttcacc attttgtaag gatgaagaac atagcttctt atctgtgaac cccattcaat
     4261 tttcatcttt gatccttcaa gttcagcctg cttctcccgc ctctttctca attcaagttc
     4321 atagagatga gatttaagaa tcctcaatgc attttgcctg ttatccagtt gagagcgggt
     4381 ttcagtattt tctacagaaa tacctgtagg aatatgtcta acccgaacac ctgtctccac
     4441 tttatttaca ttttgacctc cggccccact ggagcgaaag gtatcccatt ctaaatctcc
     4501 aggatttatt tcaatctcta tagtgtcatc tacagctgga taaacaaaaa ctgaagaaaa
     4561 tgtggtttgt ctctttccct gagcattaaa tggcgaaatt ctgacaagcc tgtgaactcc
     4621 tgtctctgcc ttcaagtaac catacgcata ttcaccttca aattcaagtg tcacagattt
     4681 aattcctgcc tcatctcctt ctataagatc atagatatga accttgtaat tatttctctc
     4741 accccatctt gcatacattc gcatcaacat tgaagaccaa tcattacttt cagtacctcc
     4801 tgcacctgag tttatcttga gaattgctcc aagattatca ccctcttcac ctaacatctt
     4861 cctcatttcc aactcttcga ttagtctcaa agtttgagag aatgccttat ccacttcttc
     4921 ctcaagttca ggatcctccc tgacaaggtc caatagtatc tcaaaatcct ccaatgatgt
     4981 ttctatagat gcaaatttat cagtccaata cttaattgat gagacatttt ttaaaaatga
     5041 ttcagcactt ttgggatcat cccaaaaatc aggagatgag ctcctgtctt caagttcttt
     5101 tattttttct ctcttcccat ctatgtcaaa gagacctcct caacgcagaa gtacgttgct
     5161 ggagttcctt gaattgttcc agattaacca tttatttcaa taatgtttta ttcaggctct
     5221 aaattagcca gtgttttgtt aataactttt aaaatatctt cataagaaaa gccccttgaa
     5281 agagcaaatc tcaaaagttt ctcccttagc ttctttgttc catcatcctt tgactccagc
     5341 tttggcttca attcatttat tttcttaagg agaagttttt caagtaaggc aatgtcagat
     5401 tcagacgata tagagtccaa agcaatctta ataacagttt gaccaatacc tttgttcttc
     5461 aatgcatact caattttttt tcgcccccat cctgcaagag tagatttatc tcttacaaag
     5521 gcaaatgcat aacgttcctc gtttatataa ttgtcctcta taagtttacc aacaatttcc
     5581 aatatttcat gttcactaca gcctttgttc ctaagcttgg aattaatatc ctgaatagta
     5641 tactccctta tcgcacataa acgttgcatc tgggcaagca aagaatgatt gtcgtatctc
     5701 ttctttgcag cagactcacc tttttgtata cctccttcct gcaatgacat caaattattt
     5761 tttaattatt cttttaataa tcgcagcagc ttctccaact ataagtacaa gtgaagtaat
     5821 aagaattata agcacccaat ctctcaagct gataggctca gttctaaaaa catctcctcc
     5881 gaactgaaca atcaatacct gacctgcaac tatcaacagc ccgactaaaa gaaatccctt
     5941 actttttgta atatctctga atgcagattc tgaagaacca aatcttttgg cattgaacat
     6001 gttccaaaat tgcaacatta caaaaaaagt aaagaaccgc gacgaatcat atcgggaaat
     6061 ttcacctgca tcattagtaa agtactccaa tagtccaagc ataaaaacga caaaaattac
     6121 tcccgaagta ataataatta cagccatttt ttttgtaatt ataaaatcat cagcttttct
     6181 tggcttacgc tccattaccc gttcatcagg cggcaaggag gcaagtgctc cggctgcaaa
     6241 ggtatccata atcagattaa cccaaagcat ctgagtaaca gtcaatggga ggtccttgcc
     6301 aattaaagat cctataaaga caataagaag agctgcaaaa ttaatggtta actgaaacag
     6361 tataaatctc tgaatattct gatacaatga acgcccccac atgacagcag ttgctatact
     6421 gttgaaagag tcgtcgagga gtgttatgtc gcttgcctct tttgcgacgg aagtaccaga
     6481 gcccattgaa agaccaacat gagcataatt gagagccgga gcgtcattag ttccatcccc
     6541 agtaacagct actacagctc ctttcttctg caaaagctgc actaatcttt gcttgtctat
     6601 tggacgcgcc ctgcacatga tcttaagtcc cattacacgg tcaagagctt cctcatcagt
     6661 caaagcctca aaatccactc cggtaattat gacctcaggt ccatcattgt cattaattat
     6721 accgatttgg cgtccaattt cccatgcagt tcgcggagta tcgcccgtaa caattttaac
     6781 atcaatacct gctttaagac attttgcaac agcatccgga acatcaggcc gcacaggatc
     6841 ggcaattgcc accattccca aataagtcag gcctctttca gatagaactt ccattttctc
     6901 attacacgct tctgcaggaa tttctttgta agcaaatgcc aaagtcctca ttgcttgatt
     6961 ctgatacttt agaagcaagt cgttcaaacc atcaggtagt gaaatacatt tggaagctac
     7021 tatttcagga gcccccttaa caaaaagaat ccttctaccc aaagatgctg attcagccaa
     7081 agtagccata tatttacgtt cagtggagaa ggtaagttga tcgataatct ttgcactgtt
     7141 tctaacctcc agataattta tccctctgtc atgaagccaa agcaaaagag ctgcctcagt
     7201 aggattgcca agagccttga tcttaccatc atcagatatt tcaagaaatg cagttgaatt
     7261 tacagcaata ctctccataa taatgggttc atcattgcca actacaaagt tagtaaaggc
     7321 aactcgcatc tgattctgag tcaaagttcc tgttttgtcg gtacatataa cagttgtagc
     7381 acccatagtt tcagttgcgt gcatcttcct tacaagattg tttgtctgca gcattctctt
     7441 catactcatt gcaagactca gtgttacaga catcggcaag ccttctggca tcgcaactac
     7501 tatcaaagtt actgaaacca tgaagtaatt taaaatacgg cttgcagtat ctattgaaat
     7561 ccactgattg gcatctgaag gatcaacccc gaatatcaga taacctaacg ccattaaaat
     7621 gacagtagta gaaatcccaa atgtgaccca tcggctccaa tcactctctc tcaccaatgc
     7681 aggagttgta acttttgccc ccagaacaga caaaccgtca taaatagcag gtatccaaat
     7741 ctttgtaatt gcaataacca ctccggttat gactgaaaca agcagaaaaa tttcactgaa
     7801 agaatagttc acattaccaa gaaacatgtc ctttacaaac aacatggcaa atgtcatgac
     7861 tgcaagaaca aatccagcaa aacccacaaa ttttgccaat ttgtcaagtt gcctgttcaa
     7921 cggagtctct tcagggtttt tctcagctga tttctgtgcc acctttccat attcggtaga
     7981 atcaccaaca gcaaaaaccc tggcaatgca atgaccatct actattgtag tccccctgta
     8041 gatgttgttc gtgggataag ttgcttcatc atcaaacatg gcaggatcta cgctcttttc
     8101 tataacaggc tctcctgtca atgtagactc gttgaccgaa agagaaactg cctcaatcaa
     8161 ggttgcatca gcaggaacct cttcaccagt atcaagcaga ataatatctc caactacaat
     8221 gtcttttttg ggtacttctc taatattacc gtctcttata accttaacaa gagtttcatc
     8281 attaacctga tttaaaatat cgaacttctt gctggcgtcc atttcaaacc agaaggatac
     8341 gccggttgca agaaatatag cacagaagat acctattgtt tctacaaatt cattgtgaac
     8401 aaatgatata cctaatgaaa gaaatgcagc aacaagcaaa atcctgatta ttggatcatt
     8461 aaattttaaa agaaataatt tccaaagagg atctctcttt ggaggagtaa gcacattgct
     8521 gcc
//
LOCUS       DAOTQL010000073         8523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7210_length_8523_cov_4.371162, whole genome shotgun sequence.
ACCESSION   DAOTQL010000073 DAOTQL010000000
VERSION     DAOTQL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7210_length_8523_cov_4.371162"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1217
                     /gene="ychF"
                     /locus_tag="P5293_05390"
     CDS             114..1217
                     /gene="ychF"
                     /locus_tag="P5293_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRR49369.1"
                     /translation="MSLQCGIVGLPNVGKSTLFNCISSGKAQAANFPFCTIEPNIGST
                     LVPDYRLQVLEQLVHPNRVVPATVEIVDIAGLVKGASKGEGLGNQFLANIRETDAIIH
                     VLRCFDDPNVVHVDGSVNPLRDKEIVDTELQLKDLDTIESRMNKVIKQAQTGGDKTAK
                     RLYEVLLKYKTALEQGRSARTVVFDNLEDKKLAKDLFLLTDKPVLYVCNVDESSAKSG
                     NTYVDQVRDAIKDENAQMLVVAAKIEAEIAELESYEDKKLFLNEIGLQESGVNRLIRA
                     AYKLLNLETYFTAGVQEVKAWTFHKGTKAPQAAGIIHTDFEKGFIRAEVIKYEDFIKY
                     GSEAACKEAGKINVEGKDYVVQDGDIMHFRFAI"
     gene            complement(1244..3343)
                     /locus_tag="P5293_05395"
     CDS             complement(1244..3343)
                     /locus_tag="P5293_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRR49370.1"
                     /translation="MAVLEKIRVKMGVFITLIIAIALLSFIIDPGTLQSVLSIFSSKY
                     DVGEMNGKSITYQEYQKKVDYFTKIFQLTSGSSTMSDETQEYVNQSAWQELITENVLL
                     PVMDMAGVKVGDDEMVDLSQGTNISPVLLREQTFIGENGQFDRSRLVQFIKQIPQDNS
                     GNLSTYWKFLENNIRTEQIFSKYISLLVKSSIYNPVELRRIIEENNVTSDVSFVLQPY
                     AFAPDSTVTVSKQEIKDYYNKYKRHFYRKASRDIEYVVFNVVPSETDMELAKKSIDNL
                     FDEFATTDNLKNFLAKNSDKPLDPYYYKEGELAAVSPILDSFAFKAPVNAVLPVFLQD
                     YTYMSARINDKKQMPDSVYVQHILIQNSDRSAANKLADSLIQLLSKGVDFSELAAKYS
                     ADKNPNVTPGDIGWMTQQQLIPGFEPCFDLAQGKITKLETDYGIHVVKVKEMSKPVTK
                     VQLAVLVKEALASKETYQKYYSQANDLATKANGNIEKFNAAVKELNLIAIPASGIEPG
                     AKNVATYKNAREISRWAYEAKVGDVSNIIAVDNKYFFVVALTAAYEEGIPEMSAVEPK
                     IVEIIKKEKSDKKAVAEVAEKIKGLATMDEIANALGLTISKQEGISFGALGSQSFDPK
                     FIGSVCGAEVNKVTGPIDGTVGVYVFNVDARQTGAFFTEDDAKARNNQILGYKMQMLS
                     PILEKAANVKDTRYKFF"
     gene            complement(3411..>4430)
                     /locus_tag="P5293_05400"
     CDS             complement(3411..>4430)
                     /locus_tag="P5293_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRR49371.1"
                     /translation="EIVDSMTKERKRATSSLRIGKMVFLLIFGISFYILTYKPLCSLF
                     HDLNYVVLLIEIFLIVFIVLVISEVIPIIVGSVNPNRILKLFYRVASISFKIFSPFVS
                     KEIVMREEPKDQDAVLIQNALGFSDVLLKKCMIPRTEVCAVEEETTYAELLDLFAKTD
                     YSRIIVYRESIDTITGYVHSKDLFKGERPVRELIRKIGYFPETTHARELLETLIKSHS
                     TIAVVIDQFGGTAGIVTLEDLFEEIFGEIDDELDNDELIEIRQENGEFIFSGRIEVKY
                     LNREYDLGIPESEEYETLAGFITFCNENIPVAGDVIKYENLVFTVLKTSSNRIETVSL
                     RIVDE"
     assembly_gap    4431..4530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4531..5045)
                     /gene="lptC"
                     /locus_tag="P5293_05405"
     CDS             complement(<4531..5045)
                     /gene="lptC"
                     /locus_tag="P5293_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRR49372.1"
                     /translation="MSSGSNPRYLKQMFVAATFVVATLVLSCKSDIPAADPISAEEFP
                     TQIVDNMYFQQTENGVVVLRVFAKKMERYTNAKEPYEYFPEGINVKAFTRDGALETEI
                     RANAAKHKTAANNEVWEAYGNVVINNYMKGEEMVTDTLYWNRLTKKIYTHCVVKLKSP
                     DVFMQGIGMES"
     gene            complement(5048..>5982)
                     /locus_tag="P5293_05410"
     CDS             complement(5048..>5982)
                     /locus_tag="P5293_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49373.1"
                     /translation="DQMVMDAFNETLKVCGDMTESDVLVAGMGKIYDMYTKKTATEED
                     VLNMYSRISQILDNQIKSEDADADVKTQAMSAKQKVDLIFVNSGVANCDNIVALYSPK
                     FKENPTDKDVVAIIVKLLGDAGCFSEDLYIKAVEELYKIDPSYKSASALAKLYVMRND
                     FANAIKYLQEAIDSPESNDIEDGSLLMDMATYYVKLEQFNKAAEVAREAMQKNPSVAG
                     QAYMIIGTAWAMARCGGNEIESRAHFWVAVDYFIKAKNADSSLAADADKMIRQFSQYF
                     PLQEEAFMYDITDGSSFTVNCNGMSATTTVRTRK"
     assembly_gap    5983..6082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6143..7381)
                     /locus_tag="P5293_05415"
     CDS             complement(6143..7381)
                     /locus_tag="P5293_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49374.1"
                     /translation="MVAQTTDALSTYTPYSLFGVGDVSRPGTAFNKSMGSIGIGVKDM
                     RYINYLNPASIVERDSLSFMADFGMEQNNHYLSGNGVKSAYNSFNMHHLLITLPIYKR
                     STFIIGAVPFSSVGYKFAKKETDPEIINEMGDVEYQKYGTGSISQLLAGASVKIKNFA
                     IGAEGIYYFGTIDRYSNVVFSSSTTYRQILTGYDYVVGAFSGKFGLQYTQKIKPEIEA
                     TMGITYSLKTKLRGDMTRYAKATDIAGNLDTILFDKKNDPAMSVPAELGIGFSVKKSE
                     KWMFGADYVIQNWSGINFTETPGIDFKAVNSSAFRIGAEYTPNLYDIRYYYKRITYRA
                     GLYYDQSYMKVLGSQVNSLGFTLGMNFPISRWFNSLGLTLDVGQRGTSSDKLVKENYF
                     MVGISFSLHDISWFRKVKYD"
     gene            complement(7437..8156)
                     /locus_tag="P5293_05420"
     CDS             complement(7437..8156)
                     /locus_tag="P5293_05420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRR49375.1"
                     /translation="MNLLVDIGNSSVKAVYASKGNFSPVYSCFNDNYFEFIYDLISDE
                     KPDRIFISSVRGFDGGEKVKLKETCDELIVVDSKINLPIKINYSTPNTLGPDRICAAV
                     GASCFFPKMDVIIFDFGTALTIDRLSASGEFCGGNISPGMMTRIRALHDYTHMIPMID
                     IPDKITEMGKSTKEAVESGIILGILFELEGYMNRNPDSVYIFSGGDAIYFAKRMKRSI
                     FVVYNLVIMGLARIADYHAEF"
     gene            complement(8177..>8523)
                     /locus_tag="P5293_05425"
     CDS             complement(8177..>8523)
                     /locus_tag="P5293_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRR49376.1"
                     /translation="DIEIDKFLEEMKDRFGPVPSQVEQLTYTVKLRRIAVGLGFERIT
                     LKNGIMLAYFVSNQLSSYYQSNKFAKILSFLQTKSKRFKLKEQRDKLYISVENVKSME
                     SAYKILLEMNNI"
BASE COUNT         2676 a         1664 c         1272 g         2711 t
ORIGIN      
        1 ctgtaaatac taagggcatt ttatgttaat tatctgattt catggcgttt ttgtctatta
       61 ttgagttttt tgacaaaaat taattaattt cgaacattat aaaattagag aaaatgtcac
      121 tgcaatgtgg tatagtaggc cttcctaatg tcggaaaatc aacacttttc aattgtataa
      181 gttcaggaaa agctcaagca gcaaattttc cgttctgcac tatagaacca aatattggct
      241 caaccctggt tccagattat agacttcaag ttcttgaaca gcttgttcac ccaaatagag
      301 ttgttcctgc aactgtagag attgtagaca ttgcaggact tgtaaaagga gccagcaaag
      361 gagaaggcct cggcaatcag tttcttgcca atattaggga aactgatgcc attattcatg
      421 ttttaagatg ttttgatgat ccaaatgttg ttcatgtaga tgggagtgtc aatcctctaa
      481 gagataagga aatagttgat actgaacttc agcttaagga tctcgatact attgaaagca
      541 gaatgaataa ggtaataaaa caggctcaga ccggaggaga caaaacagct aagagactct
      601 atgaagttct tcttaaatac aaaactgctc ttgaacaggg tcgctcagca aggactgttg
      661 tttttgacaa tcttgaagat aaaaaacttg caaaagatct gtttttgctt actgataaac
      721 ctgttctata tgtatgtaat gtcgacgaat cttctgccaa aagtggaaac acttatgttg
      781 atcaagttag agatgcaatt aaagatgaaa atgcccaaat gttggttgta gctgcaaaaa
      841 ttgaagcaga aattgcagaa ttggaaagtt atgaagataa aaagttattt ctcaacgaga
      901 taggtttaca ggaatctggt gttaaccgtc ttataagagc agcatacaaa ttacttaatc
      961 tcgaaaccta cttcactgcc ggtgttcaag aagttaaagc ctggactttt cacaaaggga
     1021 ctaaagctcc acaagcagca ggaattattc atactgattt tgaaaagggc tttatcaggg
     1081 ccgaggtcat taaatatgaa gattttataa aatatggttc tgaagctgca tgcaaagaag
     1141 caggaaaaat taatgttgaa ggaaaagatt atgttgtaca agacggcgac attatgcatt
     1201 tccgttttgc aatataaaat aaaactctga cgataagttt aaattaaaag aacttatacc
     1261 tggtatcctt gacatttgct gccttttcaa gaatcggaga taacatttgc attttataac
     1321 ctaaaatttg attatttctt gcctttgcat catcttctgt aaagaaggca cctgtttgtc
     1381 ttgcatctac attgaacaca taaacaccta cagtcccatc aataggtcct gtaactttgt
     1441 tgacttcagc tccacacact gatccaataa atttagggtc aaacgactgt gatccaagag
     1501 caccaaaaga aattccttcc tgtttactta ttgttaaacc caaagcattc gcaatttcat
     1561 ccatagtcgc aagtccttta atcttctccg caacctctgc gacagctttc ttatcactct
     1621 tttctttctt gataatttcg acaatttttg gttcaacggc actcatttct ggtatcccct
     1681 cttcataagc agctgtaagt gcaacgacaa agaaatattt gttatctaca gcaataatat
     1741 tagaaacatc tcccactttt gcttcatatg cccaacgact tatttcacga gcatttttgt
     1801 aagtagcaac attttttgca cccggttcaa tacctgaagc aggaatggca attaaattta
     1861 actccttgac tgctgcatta aatttttcaa tattgccgtt tgccttagta gcaagatcat
     1921 ttgcttgaga ataatatttt tgatatgtct ctttacttgc caaagcctct tttaccaaaa
     1981 ctgccagctg aacttttgtg accggcttgc tcatttcctt aaccttaacc acatgaatgc
     2041 cataatctgt ctccagcttg gttatcttgc cctgagcaag atcaaaacaa ggttcaaatc
     2101 caggaattaa ttgttgttga gtcatccatc caatatcacc aggagtaaca ttagggtttt
     2161 tgtcagcaga atatttagcg gcaagttcag aaaaatctac tcccttggag agaagttgaa
     2221 taagagaatc agccaattta tttgctgcag acctgtccga attctgaata agaatatgtt
     2281 gaacataaac ggaatcaggc atctgcttct tatcatttat tcttgccgac atatatgtat
     2341 aatcttgaag aaaaacggga agtactgcat ttacaggtgc cttaaaggca aaactgtcca
     2401 gaatcggaga tacggctgcc aattctccct ctttgtaata atatggatct aaaggtttat
     2461 cggaattttt ggccaaaaaa ttcttaagat tatctgtagt tgcaaattca tcaaaaagat
     2521 tatcgatact tttctttgcc agttccatgt ctgtttcgga agggacaaca ttgaatacta
     2581 catattcaat atctctgctt gctttcctgt aaaaatgtct tttgtattta ttgtaataat
     2641 cctttatttc ctgctttgag acagtaactg tgctatcagg agcaaatgca tatggttgca
     2701 atacaaatga gacatcggat gtaacattat tctcttctat tattcttctt agttcaacag
     2761 gattgtagat tgagcttttt acaagaagag aaatgtattt cgaaaatatt tgctcagttc
     2821 tgatattatt ttcaaggaac ttccaatatg ttgaaagatt accggaatta tcctgaggta
     2881 tttgctttat aaattgaacc agtctggatc tgtcaaactg accgttttca ccaataaaag
     2941 tttgttctct caaaagcaca ggagaaatat ttgtcccttg gctgagatcc accatttcat
     3001 catcgccaac tttaactccg gccatatcca ttacaggaag taaaacattc tctgtaatga
     3061 gttcctgcca ggcactttgg ttaacatatt cctgagtctc atcactcatt gtagatgaac
     3121 cggaagttaa ctgaaatatc tttgtaaaat aatcgacttt cttctgatat tcttgatatg
     3181 ttattgattt accattcatt tctccaacat catactttga agaaaaaatt gaaagaactg
     3241 attgtaacgt tcccggatct ataataaaag ataaaagtgc aatagctata attaaagtaa
     3301 taaagacacc catcttaacc cggatctttt ctagaactgc cattgtgtat taattttaag
     3361 taatttcatg tttggccgcg aagatacgta aaaaaatcat tttacctccc ctattcgtca
     3421 actattctaa gtgaaaccgt ctctattctg tttgatgaag tcttcagtac agtaaaaact
     3481 aaattttcat acttaattac atctccagca accggtatgt tttcattaca aaaggttata
     3541 aaaccagcaa gtgtttcata ttcttcagat tctggaatgc cgagatcata ttctctattt
     3601 aaatatttaa cctctatcct gccggaaaaa ataaattcac cgttttcctg tcttatttct
     3661 ataagctcat cgttatccaa ctcgtcatca atttctccaa aaatctcctc aaaaagatct
     3721 tcaagagtta cgattcctgc tgtgcctcca aattgatcaa ttacaacagc aattgtactg
     3781 tgactcttga taagcgtttc aagaagctcc ctggcatgtg tcgtttcagg gaagtatcca
     3841 atttttctta ttagctccct aacgggtctc tcgcctttaa aaagatcctt ggaatgaaca
     3901 tatcctgtaa ttgtatcgat actttcccta tatacaatta ttcttgaata atcagttttg
     3961 gcaaacaaat ctagaagttc agcataagta gtctcctcct caactgcaca tacctcagtc
     4021 cgtggtatca tacatttctt caacaataca tcagagaaac caagtgcatt ttgaattaag
     4081 acagcatcct gatcttttgg ttcctctctc ataacaattt ctttactgac aaatggggag
     4141 aaaatcttaa atgatattga agcaactcta taaaataact tgagaattct attcggatta
     4201 acagaaccaa caataatcgg aatcacttcg gaaataacca gcactataaa tacaatcaaa
     4261 aaaatttcta taagaagcac tacataattc aaatcatgaa aaagtgagca taatggttta
     4321 taggtcaaga tataaaacga aatgccgaat atgagcaaaa aaaccatttt accaattctc
     4381 agcgatgaag tggctctttt cctttccttt gtcatactgt caacaatttc nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctgactcca tacctatacc ctgcataaaa
     4561 acatccggag acttaagctt tacaacacaa tgagtatata ttttttttgt aagtcggttc
     4621 caataaagcg tatcagtaac catttcttca cccttcatat aattgttaat tacaacattg
     4681 ccgtaagctt cccaaacttc gttatttgca gctgttttgt gctttgctgc atttgctcta
     4741 atctctgtct caagagctcc gtctcttgta aaagctttga cattaattcc ctccgggaag
     4801 tattcatatg gttctttagc atttgtatat cgctccattt tttttgcaaa gactcttaaa
     4861 accacaactc cattttctgt ctgttgaaaa tacatattat caactatttg agtcggaaat
     4921 tcctcggcag aaataggatc agcagcagga atatcacttt tacatgataa aacgagtgta
     4981 gcaaccacaa aagtggctgc tacaaacatt tgtttcaaat atcgaggatt acttcccgaa
     5041 gacataatta tttccttgtc ctaactgttg ttgtggcaga catgccgtta cagtttacag
     5101 taaatgaact tccgtcggta atatcataca taaaggcctc ttcttgaagt gggaaatact
     5161 ggctgaactg tctgatcatc ttgtcggcat ctgcagctaa tgaagaatca gcattctttg
     5221 ctttaataaa ataatcaaca gctacccaga aatgagctct tgattcaatc tcgttgcctc
     5281 cacatctagc cattgcccaa gctgtaccaa taatcatata tgcctggcct gcaaccgaag
     5341 gatttttttg catcgcttct cttgcaactt ctgcagcttt attgaattgt tccagtttaa
     5401 cataatatgt tgccatatcc attaacagag aaccgtcttc aatatcattt gactccggac
     5461 tgtctattgc ctcttgtaaa tatttaattg cattagcaaa atcattacgc attacataaa
     5521 gcttggcaag tgcggatgcc gatttataag atgggtcaat cttgtacaac tcctcaactg
     5581 ctttaatgta aaggtcttca gagaaacatc ctgcatcacc gagcaatttg acaataattg
     5641 caaccacatc tttatcagta ggattctcct taaacttagg actataaaga gcgacaatat
     5701 tgtcacaatt tgccacacca ctgtttacaa aaattaagtc aaccttttgt ttagcactca
     5761 ttgcttgtgt cttgacatct gcatctgcat cttcactctt aatctgatta tctagtattt
     5821 gagatattct tgagtacata tttaatacgt cttcttcagt agcagttttt tttgtgtaca
     5881 tatcgtaaat tttacccatg cctgcaacaa gaacatcgct ttctgtcata tctccacaca
     5941 cttttaaagt ttcgttaaaa gcatccatta ccatctgatc atnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnaataaagt gcgcgctttt ctcatagctg atatttttta
     6121 aatttcaaat tttctataat tattaatcat attttacttt acggaaccat gaaatatcat
     6181 gtaacgaaaa actaattccc accataaaat aattttcttt aacaagtttg tcagaagatg
     6241 tgcctctttg accaacatcc aacgtcaaac ccaatgagtt gaaccatctc gaaataggaa
     6301 agttcatccc caaagtgaag ccaagtgaat ttacctgact tccaagaact ttcatgtatg
     6361 actgatcata atacagacca gctctatagg taatcctttt ataatagtaa cgtatatcat
     6421 aaaggtttgg agtatactca gcccctatcc tgaaagcaga actattaact gccttgaaat
     6481 ctatacctgg agtttcggta aaattaatgc cagaccagtt ctgaattacg taatcggcac
     6541 caaacatcca tttttcactt tttttaacag aaaagcctat acccaattca gcaggcactg
     6601 acatcgctgg gtcatttttc ttatcaaata agatagtatc caaattgcct gctatatcag
     6661 tcgcctttgc atatcttgtc atatcacctc tgagtttggt cttaagagaa taagtgatac
     6721 ccatggttgc ttctatctca ggctttatct tctgcgtata ctgcaatcca aatttgcctg
     6781 aaaaagctcc aacaacatag tcatacccag taagaatttg cctatatgtt gtgcttgaag
     6841 aaaaaacaac attggaatat ctgtcaattg taccaaaata gtatattccc tctgcgccaa
     6901 ttgcaaaatt ctttatttta acagacgccc cggcaagtag ttgacttatg cttccagtac
     6961 catatttttg atattcaaca tcgcccattt catttattat ttcaggatcg gtctcctttt
     7021 tggcaaattt ataacccaca ctactaaaag gaacggcacc aataataaaa gttgatctct
     7081 tgtagatcgg taaagttata agcaaatggt gcatattgaa actgttataa gctgatttta
     7141 caccattgcc cgaaagataa tgattattct gttccatgcc aaaatcagcc ataaacgaaa
     7201 gagaatctcg ttccacaatt gaagcagggt ttaaataatt aatatatctc atatccttaa
     7261 ctccgatgcc tatacttccc atgctcttgt taaatgcagt accaggcctt gaaacatctc
     7321 ccacgccaaa aagcgaataa ggggtataag tacttagtgc gtcggttgtc tgtgcaacca
     7381 ttttgctaca aataataaaa aaagtgagta ttaaacacaa ccttttccct ttcactttaa
     7441 aactctgcat ggtaatctgc aatccgggct aagcccatta taactaaatt ataaaccaca
     7501 aatatcgacc ttttcattct tttggcaaaa taaatcgcat cacccccaga aaaaatataa
     7561 actgaatcag gattacgatt catataacct tccaattcaa atagtattcc caaaattatg
     7621 ccagactcaa cagcctcttt tgtagatttt cccatttctg taattttatc gggaatatcg
     7681 atcatgggaa tcatgtgtgt ataatcatgc aaagctctta ttcgtgtcat catacccggt
     7741 gaaatatttc caccgcaaaa ctctcctgaa gctgataatc tgtcaattgt aagcgcagta
     7801 ccaaaatcaa aaattattac atccatcttc ggaaaaaaac atgatgctcc aactgctgca
     7861 caaattctgt ctgggccaag tgtattggga gttgaataat taattttaat aggaagattt
     7921 attttagaat ctaccactat caattcatca caagtctctt taagtttaac tttttctcca
     7981 ccatcaaaac ctctcacaga tgagataaaa atcctatcag gcttttcatc actaattaaa
     8041 tcatatataa actcaaagta attgtcatta aaacaactgt atacaggact aaaattacct
     8101 tttgaagcat atactgcttt tacagaactg ttccctatat caactaatag attcattaaa
     8161 aaatataaag ataatattat atgttattca tctcaagtaa aattttatat gcactttcca
     8221 tactcttgac attctcaact gaaatgtaca atttatctct ttgttctttc aatttaaaac
     8281 gcttggattt ggtctgtaaa aaagacaaaa tttttgcaaa cttattagat tgataatagg
     8341 aagataattg attacttacg aaataagcca gcataatccc attttttaaa gtaatcctct
     8401 caaaaccaag tccaactgca attcttctaa gttttacagt gtatgttaat tgttctacct
     8461 gactcggaac aggaccaaat ctatctttca tctcctcaag aaatttatct atctcaatat
     8521 cct
//
LOCUS       DAOTQL010000074         8369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7332_length_8369_cov_5.493625, whole genome shotgun sequence.
ACCESSION   DAOTQL010000074 DAOTQL010000000
VERSION     DAOTQL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7332_length_8369_cov_5.493625"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="P5293_05430"
     CDS             <1..281
                     /locus_tag="P5293_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRR49377.1"
                     /translation="VNCIVLGRAEAFASKNSVMDSMLDGIGIGLGFTLSLTVIGIVRE
                     LLGSGSLFGFKFIGGDGMLAFILAPGAFIVLGYLMVLFNIYKSKTSGK"
     gene            287..886
                     /locus_tag="P5293_05435"
     CDS             287..886
                     /locus_tag="P5293_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HRR49378.1"
                     /translation="MEYIIIIISAVFVNNIVLTQFLGICPFLGVSNKLSTAMGMSGAV
                     VFVIALSTLVTYLLQYYVLVPLGIEFMQTVAFILVIAALVQMVEIILKKISPSLFKAL
                     GIFLPLITTNCCVLGLAIIVVAKEFSFGGEPHMLNLAESVVYATASAIGYGFALILFA
                     GLREQLELNNVPKVLKGTPIALITAGILALAFMGFSGLV"
     gene            899..1180
                     /locus_tag="P5293_05440"
     CDS             899..1180
                     /locus_tag="P5293_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR49379.1"
                     /translation="MKKEKSLLEIEQMLRDGSIQKAHEELLKLLDREPDNDSAWYMLG
                     GLFRREQRWGEAINALNRAKYLNPDGPAAHAIDSIYEILRFQNNDFMNP"
     gene            complement(1153..1698)
                     /locus_tag="P5293_05445"
     CDS             complement(1153..1698)
                     /locus_tag="P5293_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRR49380.1"
                     /translation="MFKKLHVNELGRLSIEEFKQSPKIPVVIVLDDIRSRHNIGAAFR
                     TADAFAVESIVLCGICATPPNNEIHKSALGAEETVTWKYESDILNAITNLKAKGYRII
                     CAEQTENSTQLQNLTIERDKKYAVVFGNEVKGVRQEVLDICDVAVEIPQFGTKHSLNV
                     SVSIGIILWEMISQGFMKSLF"
     gene            1765..1935
                     /locus_tag="P5293_05450"
     CDS             1765..1935
                     /locus_tag="P5293_05450"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRR49381.1"
                     /translation="MNSEVKLTLIQNGPLKVEGEVVIVHPDGKEEKKNSCYICRCGKS
                     NNKPWCDGSHAK"
     gene            2015..3127
                     /locus_tag="P5293_05455"
     CDS             2015..3127
                     /locus_tag="P5293_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR49382.1"
                     /translation="MKKRGKFILYLSIPVVFVIAVFIYKSTFSGVKMGDGSGHQGNSE
                     VNRAKSRGEGEKSLPVSVHIADLHLIEDGLTLPGTLVARERVEMASELAGRVVSINFK
                     EGEFVKKGEILVKLNDDELQAQLVRAQYQYDLLSQKLERQKILLSKEAVSREEYDQAS
                     TEFNVLKQDIEQLEIKIEKMKIRAPFDGVIGFRNISLGALLVQGSKVATIVDVVHLIL
                     EFSIPEKYVRNQLIGNSVTFTIEGMGNTFSALIYAIDPEVDVQTHTMTLRANFDNSKG
                     LLKPGMYAKVSVVSQKKIPSVYVPNQAIVSGVKGHSVWILKDGKASMTQIQTGLRTVD
                     MMEVISGVEKGDTVITTGLMQLREGMSVTPTNLKSE"
     gene            3124..6177
                     /locus_tag="P5293_05460"
     CDS             3124..6177
                     /locus_tag="P5293_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRR49383.1"
                     /translation="MSLSSTSINRPVLTIVMSIIIVIFGVIGYVTLGVRDYPSVDNPV
                     ISVRCSFPGANSDVIETQITEPLEAAVNGIPGIRSISSTSRDGQSSINIEFNLDVDLE
                     TAANDVRDKVSGAMRRLPRDIDAPVVAKSDADAQPIFSVTLQSGQRDIIDLSEYAEVN
                     FKERLQTISGVSNVDVWGSKRYSVRLRMDPLLLAAYKVTPLDVRNAVNNENVELPSGT
                     ITGSTMELTVRTQGRLRTIEDFNNLIISKNGGRIVRFSDVGVAEVDAENRRSIQKRNG
                     VPMVSCVLIPQPGANYINIVDDAKKAVEELKKSLPDDIEVGVGFDNTIFIRDSIKEVK
                     TTILQAFILVVLIIFLFLRNWRTTLIPILAIPISLIGVFFIMYLVGFSINILTLLAVV
                     LSIGLVVDDAIVVMENIYTKIERGIPPKKASLEGCKEIFFAIIATTVTLVFVFFPIIF
                     LQGVTGRLFREFAVVIAGSVLISCFVALSFTPMITSKMIKSGVYEGGFYKKTEPFFVW
                     LNNAYRNSLNFVMRRRWVAPVFLVITVGLIVWLWTSIPSEMAPLEDRSMISISCTAQE
                     GATFDYMLQYSDEMWDMIRVSVPELENAMQNVGMGGNNRASFQLSLVPPDKRKKTQQE
                     IAEELAPKVAMMTGAKSFVTQQQSFGSRRGGMPVQYVIQARNLDLLKEILPEFMTEVS
                     HSNVFSNSDVNMKFTKPELSLSIDRDKTSLMGVSIQNIAQTIQLTMSEQRIGYFIRNG
                     KQYQILSEINQDDRNKPLDMANIFVRNDAGNLIALDNLVTMEESSMPPQLYRYNRFVS
                     ATVSASLAKGYTLGQGIEEMDRISKKVLDHNNFSTTLSGQSKDFVESTSSLLFAFLLA
                     LILIYLVLAAQFESFRDPLVIMLTVPLAIVGALLCLKAASQTMNIFSEIGLIMLIGLV
                     SKNGILIVEFANQRRAAGIEKRKAVLESAAARFRPILMTSLSTILGIMPMVFATGSGA
                     ESRIAMGVSVAGGLIFATFFTLYIIPVMYSYISSSKDLALRNENE"
     gene            6170..7489
                     /locus_tag="P5293_05465"
     CDS             6170..7489
                     /locus_tag="P5293_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR49384.1"
                     /translation="MNKSVIILLMSAILSLLHISTRGQENVKLQDCIAIALKDNYSVT
                     ISSNELSISKNNISLAPFLPTVTLGSKQSTSELHERSYNAQGEVGKDISKATTILSSA
                     NLNWRLFDGFYMFAEREKQRELLQQGEFSFRSVVENLVMDISSQYYKIISLQNQVKLL
                     DELVGISNTRYNQALTRYKIGKDSGLEYMQAKINLNSDSSQLLLQRENLKNAYIELFR
                     LMNVDLNSSYIVKDTIVPEPMMSLENILVAGFQNNTSILAARVGERVAGLELKIAKSS
                     RYPTLDFSTGYNYNLYDNDYFPSKYDRYNGYNFGFNLSVPIFDGLEVNRKIKNAKILQ
                     ENTRLSFEKTKQDLESQLRSVYNLYTNNIRMVDFESENKEAAFLNLEAAMEKYRLGSL
                     SGIEFRDIQLSYLDASNRMLDAIYQAKISEITLHLLMGDLFQPVPQK"
     gene            complement(7437..>8369)
                     /locus_tag="P5293_05470"
     CDS             complement(7437..>8369)
                     /locus_tag="P5293_05470"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRR49385.1"
                     /translation="LDQKPFAKGGILDISAFQNIKDKRVRDITIEQLLRHRAGFSRRR
                     GDPMFIPNTIRKKMNLDSAPTTDDIIRYVLGSTLDYQPDSGTKYSNFGYLLLSRIIEQ
                     VTGMPYESYVKNIILSPMGIHDMHIARNFHDQKFPNEVRYYEPPDELPIESFDGSGML
                     CPKCYGGNDVAGLLGAGGWVASPTELIYFIAHIDGRPHIPDILSEESITIMTTADDNY
                     LPIGWAKVRSNGDLIRTGTLSGMSAMVKCQRDGLTWVFLANTSSWKGARFPKIVEGMF
                     NKAFSIVDNLPERDLLEITSEEQAETDLPSEDEV"
BASE COUNT         2481 a         1408 c         1796 g         2684 t
ORIGIN      
        1 tggttaactg tatagttctt ggtagagctg aagcttttgc ttctaaaaac agtgtaatgg
       61 attcaatgct tgacggcata ggtattggtt tgggatttac attgtcactt actgtaatcg
      121 ggatagtgag ggaacttctt ggaagtggat cattatttgg gttcaagttt ataggtggcg
      181 atggaatgct tgcatttatt ttggccccag gtgcatttat tgtccttggt tacctgatgg
      241 ttttattcaa tatttataaa tcaaaaacat ctggcaagta aaagttatgg aatacattat
      301 aattattata tctgccgttt ttgtcaacaa tattgtgttg actcaatttc ttggaatttg
      361 tcctttcctg ggtgtatcta acaaactcag tactgccatg gggatgtccg gagctgtagt
      421 atttgtaata gccctttcta cacttgtaac ttatctttta caatattatg tgctggtccc
      481 tcttgggatt gaatttatgc agacagtcgc ttttattctt gttattgcag ctctcgttca
      541 gatggtcgaa ataattctta agaagattag tccgtcattg tttaaagctt tgggaatatt
      601 tttgccttta attactacaa attgttgtgt attaggcctt gcaataattg tagttgctaa
      661 ggaattttca tttggcggag aaccacatat gctgaatctt gctgaatcgg tggtatatgc
      721 tactgcgagt gctatcggat atggatttgc tcttattcta tttgcaggat tgagggaaca
      781 gctcgaatta aacaatgtgc cgaaagtatt aaaaggtact ccaatagctt tgattacagc
      841 cggaatactc gctttggcgt ttatgggatt ttcaggattg gtttaataac aataaattat
      901 gaagaaggag aaaagcctgc ttgaaattga acagatgcta agggatggat ctattcagaa
      961 agctcatgag gagttattga aattacttga tagagaaccg gataatgatt ctgcatggta
     1021 tatgcttgga ggattattta gaagagaaca gaggtggggc gaggcaatca atgctctgaa
     1081 cagagcaaaa tatctgaatc ctgatggacc tgctgctcac gcaattgact caatatatga
     1141 aatattgaga tttcagaata atgatttcat gaatccctga ctgatcattt cccaaagtat
     1201 aataccgata cttactgaaa cgtttagaga atgttttgta ccaaattgag gtatctcaac
     1261 agccacatca catatatcca atacctcttg acggactccc tttacttcat tcccaaatac
     1321 aacggcatat tttttgtcac gctctattgt taaattctgg agttgtgtgg aattttctgt
     1381 ttgttctgca catataattc tatatccttt tgctttaaga tttgtaattg catttaagat
     1441 atctgattca tacttccatg taacagtctc ttctgcaccc aaggctgatt tgtgaatttc
     1501 attatttggt ggtgtggcac aaataccgca gaggactatg gattcaactg caaaagcatc
     1561 ggcggttctg aatgcggctc caatattatg tcggcttctg atatcatcta aaactatgac
     1621 aaccgggatt tttggggatt gtttgaactc ctctattgac aatcttccaa gttcattaac
     1681 atgaagcttt ttgaacatat ttttgaatta attgtttaca aagatatcag atatagagct
     1741 aataacaatt aaatttatca gacaatgaac tccgaagtaa aacttacctt aatacagaac
     1801 gggcctctca aagttgaggg agaagtggta attgtacatc ctgacggcaa agaggagaaa
     1861 aaaaacagct gttacatatg cagatgtgga aaatcaaaca acaaaccttg gtgtgatggt
     1921 tcacatgcta aataactaaa ggttttgatt cagttctatc attaaacttt tacttctttt
     1981 ttaagtcaaa gaaaacaagt tgatctaaaa tttgatgaaa aaaagaggta agtttatttt
     2041 atatttatcc atccctgtag tatttgttat agctgttttt atatacaaat cgacattttc
     2101 aggtgtcaaa atgggagatg gttccggtca tcaaggcaat agtgaggtta atagagcaaa
     2161 gagtcgtgga gaaggtgaaa aatctctacc tgtttctgtt catatcgccg atttgcacct
     2221 aattgaagat ggacttactc tcccgggaac tttagtagca agggaaaggg tagagatggc
     2281 aagtgaactg gccggaagag ttgtgagcat aaacttcaaa gagggcgagt ttgtcaaaaa
     2341 aggtgagata cttgttaaac ttaatgatga tgagcttcaa gctcaactgg tgagggcaca
     2401 gtatcaatat gatcttcttt ctcaaaaact tgaaaggcag aaaattttgt taagcaaaga
     2461 agctgtaagt cgtgaggagt atgatcaggc ttcgacagag ttcaatgtgt tgaaacagga
     2521 tatagagcag cttgaaataa agatagaaaa gatgaagatc agagcccctt ttgatggagt
     2581 gataggattc cggaatataa gtttgggagc tcttcttgtt cagggatcaa aagttgctac
     2641 tattgtagat gtagtacatt tgatacttga gttctcaatt cctgaaaaat atgtcagaaa
     2701 ccaattgatc ggtaatagtg ttaccttcac tattgaaggt atgggaaaca ctttctctgc
     2761 tttgatttat gcaattgatc ctgaagttga tgtccaaact catactatga ctttgcgtgc
     2821 aaattttgat aacagcaagg gtcttctaaa gcccggcatg tatgcaaaag taagtgttgt
     2881 atctcaaaaa aagattccaa gtgtatacgt tcctaatcag gcaattgtat caggagtgaa
     2941 gggtcattca gtgtggatat taaaagacgg gaaagcctct atgacccaga ttcaaaccgg
     3001 tttgagaact gtcgatatga tggaagttat ttctggtgta gaaaagggtg acacagtcat
     3061 aactacaggg ctcatgcagc tcagagaagg tatgtctgta actcccacaa atctcaagtc
     3121 tgaatgagtc tctcttcaac ttcaatcaac aggccagtgc ttacaatagt aatgagtatc
     3181 attattgtta tttttggagt aattggctat gttacccttg gtgttcgtga ttatccaagc
     3241 gtagataacc ctgttatttc tgtaagatgt tcttttcccg gggctaattc ggatgtaatt
     3301 gaaactcaga taactgagcc tcttgaggca gcagttaacg gaattcctgg aataaggtca
     3361 atttccagta caagtcgtga cggtcagagc tctatcaata ttgaatttaa tcttgatgta
     3421 gatctggaga cagctgcaaa tgacgtgaga gataaggtat caggtgcgat gcggagattg
     3481 ccgagagaca ttgatgcgcc tgttgtggca aaatcagatg ctgatgcaca accgattttc
     3541 tcagttactt tgcaaagtgg ccaaagagat ataatagatc tcagcgagta tgcagaagtc
     3601 aattttaaag agagattaca aactattagt ggagtcagca atgtggatgt ttggggttct
     3661 aagagatatt ctgtccggtt gagaatggat ccgctgttgc ttgcagcata taaggtaact
     3721 ccgcttgatg ttaggaatgc agttaacaat gagaatgtgg aactgccatc cggcacaatt
     3781 acaggatcaa ctatggagct tactgtcagg acacagggac gattaagaac tattgaagat
     3841 tttaacaatc tgattatctc aaaaaatggt ggtagaattg tccgtttttc cgatgttggt
     3901 gttgcagagg ttgatgctga gaatagacgc tccatccaaa agaggaatgg ggttccaatg
     3961 gtctcctgtg tactgattcc tcagccaggt gcaaactata tcaatattgt cgatgatgcc
     4021 aaaaaggctg tagaagaatt gaagaagtcc ttgccggatg acatagaagt tggagttggg
     4081 tttgataata caattttcat aagagattca ataaaggaag tcaagacaac tatcttacaa
     4141 gcttttattt tagtggtgtt gatcattttt ctttttttga gaaattggag aacaactctt
     4201 attcctattt tggcgattcc aatttcattg ataggtgtct tttttataat gtatctcgtt
     4261 ggattttcca taaatattct gactcttttg gcggttgtgc tctcaatagg ccttgtagtt
     4321 gatgatgcca tcgtggtgat ggaaaatatt tatacaaaga tagaaagagg catcccgcct
     4381 aaaaaggcaa gtttggaggg gtgtaaagag atattttttg caattattgc aacaactgtt
     4441 actcttgtgt tcgttttctt ccctatcata tttcttcagg gagtaaccgg caggcttttt
     4501 agagaatttg cagtagtcat tgcaggctca gtattgattt cctgctttgt tgcgctatct
     4561 tttactccaa tgattacttc gaagatgata aagtccggtg tttacgaggg tggcttttat
     4621 aagaagactg aacctttttt tgtctggttg aataatgcat acagaaattc tctaaatttc
     4681 gtcatgcgaa gaaggtgggt agctcctgtt tttttggtga ttacagttgg tttgattgtt
     4741 tggctttgga cttcaattcc ttcagaaatg gctccacttg aggacaggtc aatgatatca
     4801 atttcctgta ctgcccagga aggagcaact tttgattata tgttgcagta ttcagacgaa
     4861 atgtgggata tgatacgcgt ttcggttcct gaattggaaa acgccatgca gaatgtcggc
     4921 atgggaggta ataaccgtgc aagttttcaa ctttctcttg tgcccccgga taagagaaaa
     4981 aaaacacaac aggagattgc agaggagctt gcgcctaagg tggctatgat gaccggagcg
     5041 aaaagttttg tcactcaaca gcagagtttt ggcagcagaa gaggcggtat gcctgttcaa
     5101 tatgttattc aggcaagaaa ccttgattta ttgaaagaga tcttgccaga atttatgaca
     5161 gaggtttcac atagtaatgt gttctcaaat tctgatgtca acatgaagtt tactaaacca
     5221 gagctttctc tttccataga cagggataaa acttccctta tgggggtttc aattcaaaat
     5281 attgctcaga ctattcaact tactatgagt gagcagagaa ttggctattt tatcaggaac
     5341 gggaaacagt atcaaatctt gagtgaaata aaccaggatg atagaaataa gcctcttgac
     5401 atggctaata tatttgttag aaatgatgca ggtaacttga tagctcttga taaccttgtt
     5461 acaatggagg agagtagtat gcctcctcag ctatacagat ataatcgttt tgtctctgca
     5521 actgtatcgg catctcttgc aaaaggttat actcttggac aagggattga ggagatggac
     5581 agaatctcaa aaaaagtact tgatcataat aatttttcca caactctttc cggacagtcc
     5641 aaggattttg ttgaaagtac ttccagcctt ctatttgcat ttcttcttgc tcttattttg
     5701 atttatcttg ttttggcagc acaatttgag agttttagag atcctcttgt cattatgtta
     5761 acagttccac ttgctatagt gggagctctg ttatgcttga aagctgcttc acagactatg
     5821 aatatattta gcgagatagg ccttataatg ttgattgggc ttgtatctaa gaatggaatt
     5881 cttattgtgg aatttgcgaa tcagaggcga gctgcaggta tagagaagcg aaaggctgtt
     5941 cttgagtcag ctgctgctcg tttcagacca attctcatga ccagtctgtc aaccattttg
     6001 ggtattatgc ctatggtgtt tgcaacaggt tctggagcag aaagccgcat agctatgggc
     6061 gtatctgtag ccggagggtt aatttttgcc acctttttta cactctatat tattcctgtc
     6121 atgtattctt atatttcttc atcaaaagat ctcgctttaa gaaatgaaaa tgaataaaag
     6181 tgttataatt ctattaatgt ctgcaatttt atctcttttg catataagca caagagggca
     6241 ggaaaatgta aaacttcagg attgtatagc aattgccttg aaagacaatt attctgtgac
     6301 aatatcttct aatgaattgt caatttctaa aaataatatt tctcttgctc cttttcttcc
     6361 tactgtaact ttaggctcaa agcagtcaac ctctgagttg catgagagat catacaatgc
     6421 ccagggagaa gtaggcaagg atatttcaaa ggctacaaca atattgagca gtgcaaattt
     6481 aaactggcga ctctttgacg gtttttatat gtttgcagaa agagagaagc agcgggaatt
     6541 gcttcaacag ggagaattct cattcaggtc agttgtggag aatcttgtta tggatatttc
     6601 ttcgcaatat tataagatta ttagtttgca gaatcaggta aaattacttg atgaattggt
     6661 aggaatatcc aacacaagat ataatcaggc tttaacgaga tataagatag gtaaggattc
     6721 agggcttgag tacatgcagg ctaaaattaa cctgaacagc gactcttctc agttattact
     6781 ccagagggag aatcttaaaa atgcttatat cgaattattc cgattgatga atgtagatct
     6841 taactcttcg tatatcgtaa aagatacaat tgttccagaa ccgatgatga gccttgaaaa
     6901 catcttggtg gcaggatttc agaacaatac ttcaattctt gcagccagag ttggtgagag
     6961 agttgctggt ctcgaactta aaattgcgaa atcttcaaga taccctactt tggatttttc
     7021 taccggttac aactataatc tttatgataa cgattatttt ccttcgaagt atgacagata
     7081 taatggatat aacttcggct ttaacctgtc agtgcctatt tttgatggtt tggaagtgaa
     7141 cagaaagata aaaaacgcaa aaattttgca agagaatacc agacttagtt ttgaaaaaac
     7201 aaaacaggat cttgagagtc aattgcgctc tgtttataat ctttacacga ataatatcag
     7261 aatggttgat tttgaaagtg aaaataagga agcagccttt ttgaatctgg aagctgctat
     7321 ggagaaatac aggttgggta gtctttcggg cattgaattt agagacattc aactttcata
     7381 tctcgatgca tcaaaccgaa tgctcgacgc aatctatcag gcaaaaattt ctgaaattac
     7441 acttcatctt ctgatgggag atctgtttca gcctgttcct cagaagtgat ttccagcaag
     7501 tctctttcag ggaggttgtc cactattgag aatgctttgt tgaacatccc ttcaactatt
     7561 ttcggaaagc gagcgccctt ccaggaactt gtgttagcaa gaaacaccca ggtaagaccg
     7621 tctctctggc atttaaccat agcgctcatt cctgagagtg tacctgttct tataaggtcg
     7681 ccatttgaac gaactttggc ccaaccgatc ggtagataat tatcatcggc agtcgtcatt
     7741 attgtaatgc tctcttctga gagaatatca gggatatgag gacgtccatc aatgtgagct
     7801 ataaaatata tcaactcagt tggtgaggcg acccagcctc ctgctccaag aagtccggca
     7861 acatcgttgc caccataaca cttgggacaa agcataccac tcccgtcaaa ggactcgatt
     7921 ggaagttcat cgggaggctc gtaatatcta acttcatttg ggaatttctg gtcatggaaa
     7981 ttacgggcta tatgcatgtc atgaatgccc atgggcgaga gtattatatt tttgacatat
     8041 gattcataag gcattccggt tacttgctca ataattcttg agagtaacag atatccaaaa
     8101 ttggaatatt ttgtaccact gtcgggctga taatcaaggg tacttccaag aacatatctt
     8161 attatgtcgt cagttgtggg tgcagagtcg aggttcattt ttttacgtat agtatttgga
     8221 ataaacatcg gatctcctct ccttcgtgaa aaaccggctc tatgtctcag aagttgttct
     8281 attgtgatat ctcttaccct tttgtctttg atgttttgga atgcagatat atccaatatt
     8341 cctcctttgg cgaatggctt ttgatcgag
//
LOCUS       DAOTQL010000075         8252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7434_length_8252_cov_4.071978, whole genome shotgun sequence.
ACCESSION   DAOTQL010000075 DAOTQL010000000
VERSION     DAOTQL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7434_length_8252_cov_4.071978"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="P5293_05475"
     CDS             <1..611
                     /locus_tag="P5293_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRR49386.1"
                     /translation="KSTESDIDLYRMMFTELFDILQRSDDTTYQNCAIFVAENYIQNR
                     KELWWDNSFPGAYVPKMIERIKGNKIGAIANDFIFYTEKLKPIKLHEIRSEYTILYFY
                     SPKCAVCKPFGIELANMYKSLHKRGAYILAVDVESDFDDFKKLVKKNKSPWIRCFVKD
                     EDRMELFYKYRTEEVPMIYLLDSEKKIIAKKISTTKLNELVK"
     gene            608..>3282
                     /locus_tag="P5293_05480"
     CDS             608..>3282
                     /locus_tag="P5293_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HRR49387.1"
                     /translation="MITERYKEFYNELLKEIPSERIHHDALSTLAFGTDASFYRLIPK
                     LVVKVKDENELQLVVRKASAFDIPITFRAAGTSLSGQAISDSVLVLATHGWQKHQIIE
                     NGEKIRLQVGIRGDQANRYLERYSRKIGPDPASIDSAMIGGIAANNASGMCCGTSENS
                     YKTVADIRIIMSDGTILDTADSESRESFAKSHSCLLKGLEKLAEDIDSNEILREKIKQ
                     KYKIKNTTGYSLNAFTDYKDGFEILKHLIIGSEGTLAFISDITYKTVVEYKDKALALA
                     IYPDIEQACNAVTILKSAPVTAVEIMDRAALRSVESTKGVPEYLKSLAPNSTALLIET
                     KAKNDDELNKNISYIENSISSIKTVMPLCFTKDPSEQAILWKIRKETLPTVAGMRRSG
                     TTSIIEDVCFPVPTLAKAVLDLQTLFTKHGYSEAVIFGHALEGNMHFMFSPDFNDEME
                     FNRYRNFMEDLAEMVVEKYNGSLKAEHGTGRNMAPFVEKEWGKNALEIMKQVKQLFDP
                     KGILNPGVILNEDPQIHLKNIKPMASIRESADKCMECGFCESVCVAEGLTLSPRQRVA
                     VHREMERLRQTGKEPHRAAEIKSRYKYAALATCATDSLCAAKCPVKIDTGKLVKELRH
                     ERHSKFQENFAALISSNMRTVTAIMRFGLSVLYYIRLVLGKKLFGIIARSVRFISGGI
                     IPLWNEHFPKNAHKISKKSIVEQNKKALNSNLKVVYFPSCITRSMGISKGYRNSMDLA
                     HVTRVLLNRAGYEIIYPENLNSLCCGMAFSSKGFVKAGKTASDKLEAALTKASDGGKI
                     PILCDMSPCLYTMKSNMGERLKLYDQAEFIDKFLIHRLNIKPVDRKIALFAVCSTKKM
                     EVDENLYNIASKCAREVVRIESNCCGFAGDRGFILP"
     assembly_gap    3283..3382
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3383..5106
                     /locus_tag="P5293_05485"
     CDS             <3383..5106
                     /locus_tag="P5293_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HRR49388.1"
                     /translation="VMYKSLENFIELLDSKGELVKIKEYVDPVLEIAEITDRISKQPD
                     GGKALLFENTGTAFPVITNMFGSKRRILYALGVESFEEIENYLNTLVEEITRERHSLI
                     DKIKLLPQLKEASEWFPVNHKGDAPCQEIVLFKPELDRLPILQCWPKDGGRFITLPMV
                     HTEDPETGKRNVGMYRMQVFSQDSTGMHWHRHKTGARHFANCKTNQFPVVVTLGGDPV
                     YTYVATAPLPEGIDEYLLAGFLRNKAVELTECLTQPLKVPSDCDFVIEGYIDKSEPFA
                     IEGPFGDHTGYYSLPDLYPIFHITCISYRKDAVYPATLVGIPPQEDRFMALATEKIFF
                     TPLKLSLVPELADLHLPEEGCGHNFAFVSIKKSFPGEAIKVAHALWGAGQMMFNKIMI
                     IVDENVDVHNIESVYQAIKNNYSPKRDTHFSRGPLDVLDHASPTCGFGGKVLIDATSK
                     MIEEGGDKMEVLSYNFVSPIVFTHEEVVTKGLITVIVDQTINLEDHYTALWQWGANCD
                     PIRDSKFVGKSLVMDARHKIKGEGVREWPEMALSNIETIEIVDKKWETLELGDFIPSP
                     SRRFLKQ"
     gene            complement(5069..6676)
                     /gene="lnt"
                     /locus_tag="P5293_05490"
     CDS             complement(5069..6676)
                     /gene="lnt"
                     /locus_tag="P5293_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRR49389.1"
                     /translation="MEHNRPLHLWLLSSISAILLSLPYLVPHTGLLMLIAFIPLLQLE
                     KIIADLNIKNGWRYYYSSFLLWNILTTFWIYNATLAGAIAACILNALQMAVIFAIFRW
                     FKRKTTPSLGYIFLILAWLAWEHFYFNAEISWPWLVLGNGFATTIKNIQWYEFTGTLG
                     GSLWILLANIVIFDLLNDKNRRCKSFYIKLSLLFILIVLPLCISRIIFNSYKEKQNPR
                     EFVILQPNIDPYNEKFGALSQMQQNEILMNIANKAVTENTDFVIAPETFTSGVVENNL
                     GDNRTFIMFKEMLGEKGAGNAIFGATTFYIYPGGPDRFPPTYTARRSERGWYDSFNSA
                     IYLDTLGRTMIYHKSKLVVLVEYFPYPQYLKFLGSFAIDLGGTAGGFGTQKEREVFTT
                     LDDSIKIGTAICYESIYGDFYREYIINGANVMSIITNDGWWGNTPGYKQHLRYSSLRA
                     IETRRSIARCANTGISAFIDQRGIVTDSLGWWQRGYLKGNLNLNDRITVFVKYGDYIG
                     RGAYASMLLFVGLFILIVLKIYGKERG"
     gene            6780..7439
                     /locus_tag="P5293_05495"
     CDS             6780..7439
                     /locus_tag="P5293_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRR49390.1"
                     /translation="MITTFLKGFIVGMGASIPLGPMGIVCVQKTLSKGRHAGMIAGLG
                     SSVTDTIFAAIAILGLAYIKSMIVKYEDYLLLFGGIIVGLIGMKIFLTNPIKQIRYPM
                     TGKKHLTDFFSSMAMTLSNPGSLFLILGLFAFVGVNTDGIANEHVISSTLIGVFLGTA
                     TWWFILSATVSIFRKKFRLRQLVMINRISGIIIFILGLVSLFEGAWRLIVSYFHLNNF
                     L"
     gene            7456..7854
                     /gene="ssrA"
                     /locus_tag="P5293_05500"
     tmRNA           7456..7854
                     /gene="ssrA"
                     /locus_tag="P5293_05500"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            7929..>8252
                     /locus_tag="P5293_05505"
     CDS             7929..>8252
                     /locus_tag="P5293_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49391.1"
                     /translation="MKKLTLLSCLMFLFAGTSVAIAQNEEHSAEISTSVPELANFHKI
                     IFPMWHKAYPAKDTAALRSFIPEIKDNMAKINAAKLPGILREKEGLWKSELAKFNTVA
                     EDYYKA"
BASE COUNT         2741 a         1465 c         1582 g         2364 t
ORIGIN      
        1 ttaaaagtac agaaagtgat attgaccttt acagaatgat gtttactgaa ttgttcgata
       61 ttttgcaacg ttcagacgac actacatatc aaaattgtgc aattttcgtt gcagaaaatt
      121 atatacaaaa cagaaaagaa ctttggtggg ataactcttt tccgggagct tacgtgccaa
      181 aaatgataga gagaatcaag ggtaacaaaa ttggagctat tgcgaatgat tttattttct
      241 ataccgaaaa gttgaaacct attaaattgc atgaaattag atctgaatat actatcttat
      301 atttttatag tccaaagtgt gcagtttgta agccttttgg aattgaactg gctaacatgt
      361 acaaatcact tcataaaaga ggagcatata ttctggccgt ggatgtggaa agcgactttg
      421 atgatttcaa aaagttggtc aaaaaaaata aaagtccatg gataagatgc tttgttaaag
      481 atgaagatag aatggagctt ttctataaat acagaacaga ggaggtacca atgatctatc
      541 ttctcgacag tgagaaaaaa attattgcaa aaaaaataag tacaaccaaa ttaaatgaac
      601 tggtaaaatg atcacagaaa gatataaaga gttttataat gaacttttaa aagagatccc
      661 ttccgaaaga atacatcatg atgctttaag tacactggca tttggaactg atgcatcttt
      721 ttatagatta atcccaaaac ttgtggtaaa agttaaggat gaaaatgaac tccagttggt
      781 cgttcgtaag gcaagtgcat tcgacatacc gattacgttc agagctgcag ggacatctct
      841 ttcaggccag gcaatcagcg attctgttct tgttttggca acccatggat ggcaaaagca
      901 tcagattata gaaaacggag aaaaaatacg tcttcaagtt ggtattagag gagatcaggc
      961 aaacagatat cttgaaagat acagcagaaa gattggtcca gatcctgcat ctatcgacag
     1021 tgcaatgata ggaggtattg ctgccaacaa tgctagtggc atgtgctgtg gaacctccga
     1081 aaactcttac aaaacagtag cagacattag aattatcatg agtgacggaa caatccttga
     1141 tactgccgat tctgaaagtc gtgaatcttt tgccaaaagt cattcatgtt tgcttaaagg
     1201 tcttgaaaaa ctggctgagg atattgactc caatgaaata ctacgagaaa aaatcaagca
     1261 gaaatataaa atcaaaaaca caaccggata cagtcttaat gcatttaccg actataagga
     1321 tggtttcgaa attctcaaac atcttattat tggttctgaa ggtactcttg catttatttc
     1381 tgacatcacc tacaaaacag ttgtcgaata caaggataaa gcgcttgctc ttgcaatata
     1441 tccagatatt gaacaggcat gtaatgctgt cacaatactg aaatctgctc ctgtaacagc
     1501 tgtagaaata atggacagag ctgcactaag atctgtagaa tcaactaaag gtgtccctga
     1561 atacctgaaa tctcttgctc caaattcaac agcacttctg attgaaacta aagctaaaaa
     1621 tgatgatgaa ctgaataaaa atatctctta tatagagaat tctatctcaa gcataaaaac
     1681 agtcatgcct ttgtgtttta ccaaagatcc ttcagagcag gcaatattgt ggaaaatacg
     1741 aaaagaaaca ctccctactg ttgcaggaat gagacgaagc ggaacaactt caattattga
     1801 agatgtttgt tttccagttc cgacattggc aaaagccgta cttgatctcc aaactctatt
     1861 tacaaagcat ggatacagcg aagcagtaat ttttggtcat gctctcgaag ggaacatgca
     1921 ttttatgttt agtcccgatt ttaacgatga aatggaattc aatcgttata gaaactttat
     1981 ggaagatctt gcagagatgg ttgtagaaaa atacaatggt tctcttaaag ctgaacatgg
     2041 aacaggacga aacatggctc catttgttga gaaagagtgg ggaaagaatg ctcttgaaat
     2101 catgaagcag gtcaaacagt tatttgatcc taaaggcatt cttaaccctg gtgttatttt
     2161 aaatgaagat cctcaaatcc atctgaaaaa catcaaacca atggcatcca tcagagaaag
     2221 tgccgataag tgtatggagt gtggattctg tgaatctgtt tgtgtagcag aagggctgac
     2281 gttatcacca cgacagagag tagctgtcca cagagagatg gaacgcctca gacaaactgg
     2341 aaaagagcca caccgtgcag cagagataaa aagtagatac aaatatgcag ctcttgctac
     2401 ttgcgcgact gacagtcttt gcgctgcaaa gtgtcctgtc aaaatagata ctggaaaact
     2461 tgtcaaagag ctgaggcatg agagacattc aaaatttcag gaaaattttg cagccttgat
     2521 atcatccaac atgagaacag taacagccat catgagattt gggttatcag ttctttatta
     2581 catacggttg gttttaggta agaagctttt tggaattatt gcaagatcgg tgagatttat
     2641 ctcaggagga attatacctc tttggaatga gcattttcca aaaaatgcgc acaagatatc
     2701 taaaaaatca atcgttgagc aaaataaaaa agcactaaac tcaaacttga aagtggtata
     2761 ctttccttcg tgtattacca gaagcatggg aatctccaag ggatacagaa actctatgga
     2821 tttggcacat gtaaccagag ttctgcttaa cagagccggt tatgaaatca tatatcctga
     2881 aaatcttaat tctctttgct gtggaatggc attttcaagc aaaggatttg taaaagctgg
     2941 caaaacagct tcagacaagc tcgaagcagc tctcacaaaa gcttcagacg gtggaaaaat
     3001 tccaatcctg tgtgatatga gtccatgtct gtataccatg aagtcaaaca tgggtgaaag
     3061 gttgaaatta tatgaccagg ctgaatttat tgataaattt ctaattcata ggctgaatat
     3121 taaacctgtt gatagaaaaa tagctctttt tgctgtctgc agcactaaaa aaatggaagt
     3181 tgatgaaaat ctgtataata ttgcatcaaa atgtgcaaga gaagttgtca gaatagagag
     3241 taattgttgt ggttttgcag gagacagagg ttttatactg ccnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnaagttatg tacaaaagtc ttgaaaattt catcgaatta
     3421 ttggattcaa aaggtgaact tgtcaaaatc aaggaatatg tcgaccctgt gctcgaaatt
     3481 gcagaaataa ctgatcgtat ttcaaaacaa cccgatggag gtaaagcttt gttgtttgaa
     3541 aacacaggta cagcttttcc tgtgattacc aacatgtttg gttccaaaag aagaatattg
     3601 tatgcattag gcgttgaatc atttgaggag attgaaaatt atctgaacac tctagtggag
     3661 gagataaccc gggaaagaca ctcattaatt gataaaatca aattacttcc acaactcaaa
     3721 gaggcatcag aatggtttcc ggtaaaccac aaaggtgatg caccttgcca ggaaatagtt
     3781 ttgttcaagc cagaactcga caggttgcca attttgcaat gctggcctaa agatggagga
     3841 agattcatta ctttgccaat ggtacatacc gaagaccctg aaactggaaa gagaaatgtg
     3901 gggatgtaca gaatgcaggt cttctcacaa gattcaacag gcatgcactg gcacagacac
     3961 aaaacaggcg cacggcattt tgccaattgc aaaacaaatc aattcccggt agtagtaact
     4021 cttggaggtg atcctgtata tacatatgta gctacagctc cgcttccgga aggtattgac
     4081 gaatatctgc tggcaggatt tctgaggaac aaggctgtag aattgacaga gtgtctgact
     4141 caaccattga aagtgccgtc agattgtgat tttgtaatag aaggttatat cgataaaagc
     4201 gaaccttttg caattgaagg accattcggt gaccatacag gttattattc tcttcctgac
     4261 ctttatccaa tctttcatat aacatgtata tcctatagaa aagatgcagt ttatcctgcc
     4321 acactggtag gtattcctcc tcaagaagac agattcatgg ctcttgccac agagaaaata
     4381 tttttcacac ctcttaagct ctcacttgtt ccagaattgg cagatttgca tcttcccgaa
     4441 gaagggtgcg ggcataattt tgcatttgta agtataaaaa agagttttcc gggagaagcc
     4501 ataaaagtcg ctcatgcatt atggggagcc ggacaaatga tgttcaacaa gataatgatt
     4561 atcgttgacg aaaatgtaga tgtccataac atagaaagtg tttatcaggc tataaaaaac
     4621 aactatagtc caaaaagaga cactcatttc agcagaggtc cacttgatgt ccttgaccat
     4681 gcctctccca catgtggatt tggaggaaaa gtgctcattg atgcaacgtc aaaaatgatt
     4741 gaagagggag gagacaaaat ggaagtttta tcttacaatt ttgtgtctcc tattgttttc
     4801 actcatgagg aggttgttac aaagggactt attacagtca tagttgatca aacaatcaat
     4861 ctcgaagatc attataccgc tttgtggcaa tggggtgcca attgtgaccc aataagggac
     4921 agtaagtttg ttggaaaatc tcttgtgatg gatgcaagac ataaaataaa gggagaagga
     4981 gtacgtgaat ggcctgaaat ggcgctttct aacattgaaa ccattgaaat agtagataaa
     5041 aaatgggaaa ctcttgaact tggagatttt atcccctctc cttcccgtag atttttaaaa
     5101 caataaggat aaacaaccct acaaacaaca acatagaagc atatgcacct ctgcctatgt
     5161 aatcaccata tttcacaaac actgttatac ggtcattcaa gttcaggtta ccttttaaat
     5221 aacctctttg ccaccatccc agagaatctg ttactatccc tctttgatca atgaatgctg
     5281 aaatcccagt attggcgcac cttgcaatag atcttcttgt ttctatagct cttaaagatg
     5341 agtagcgcaa atgttgctta taaccaggtg tattccccca ccacccgtcg tttgtaataa
     5401 tagacattac attagcccca tttataatat actctctgta aaaatcgcca tatatagatt
     5461 cataacaaat agcagtacca atttttatgc tgtcgtcgag tgtagtgaaa acttctctct
     5521 ccttttgagt tccaaatcca ccagcagtac ctcctaagtc aattgcaaat gagcctaaaa
     5581 actttaaata ttgaggatat ggaaaatact caacgaggac taccaatttt gatttgtggt
     5641 aaatcatagt tctacccaaa gtgtcaagat aaattgctga gttaaaactg tcataccatc
     5701 ctctctcaga tcttcgagct gtgtatgtcg gaggaaatct gtcaggccct ccagggtaaa
     5761 tgtaaaaagt agtagcacca aaaattgcat ttccggctcc tttttctcca agcatctctt
     5821 taaacattat aaatgttctg ttatcgccca gattattttc tacgacaccg gaagtaaatg
     5881 tctccggagc tattacaaaa tcagtatttt cagttacagc tttatttgca atattcatca
     5941 gtatctcatt ctgttgcatt tgagaaagtg caccaaattt ttcattatat ggatcaatgt
     6001 ttggctgaag tatcacaaac tcacgaggat tctgtttctc tttatatgaa ttgaaaataa
     6061 tccgtgaaat acaaagaggc aatactatta aaataaataa taaggataat ttaatataaa
     6121 aagatttaca tcttctgttt ttatcattca gtaagtcgaa aattacaatg tttgcaagca
     6181 gaatccataa agatccaccc aaagtacctg taaattcgta ccactgaata ttcttgatag
     6241 ttgtagcaaa accattgccc aaaaccaacc atggccaaga tatctcagca ttaaaataga
     6301 aatgttccca ggcaagccaa gcaagtatca aaaaaatata gccaagagat ggtgtcgtct
     6361 ttctcttaaa ccacctgaaa attgcaaata tgacagccat ctgcaatgca ttcaaaatgc
     6421 aggcagctat agctcctgct aaagttgcat tgtaaatcca gaatgttgtt agaatattcc
     6481 aaagcaaaaa tgaagaataa taatatctcc atccattttt aatattcaaa tctgcaatta
     6541 ttttctcaag ttgaagaagt ggaataaaag caatcagcat taacagccct gtgtgtggaa
     6601 caaggtatgg taatgaaagc aaaatcgcag aaatagatga taataaccag agatgaagag
     6661 gtcgattatg ctccatatta ttaatatttg acaagcaaag atacaataat ctaaatatgg
     6721 attaccggct atgaaagaaa ttgactaatt ttgccactca ttattgagta taattattta
     6781 tgataaccac atttctcaaa ggatttattg tcggcatggg agcatccata cctcttggtc
     6841 caatgggaat tgtctgtgtt cagaaaacac tcagcaaagg gcgacatgca ggtatgattg
     6901 caggtcttgg atcttctgtt acagacacca tttttgccgc aattgcaatt ttaggccttg
     6961 catatatcaa atctatgatt gtcaaatacg aagattatct gctactcttt ggaggaataa
     7021 ttgtaggatt aattggaatg aaaatttttc taacaaatcc tattaagcag atcagatatc
     7081 ccatgaccgg taaaaagcac cttacggatt ttttttcgtc catggctatg acactctcaa
     7141 atccaggttc tttattttta attcttggac tttttgcatt tgtaggtgta aacactgatg
     7201 gaatagcaaa tgaacatgtc atatcatcaa ctttaattgg tgtttttctg ggaactgcta
     7261 cctggtggtt tatattaagt gcaacagtga gcatttttcg aaaaaaattc cgtcttaggc
     7321 agcttgtaat gattaacaga atctcgggaa ttataatttt cattctgggg cttgtatctc
     7381 tctttgaagg agcatggagg ttaattgttt cttattttca cttgaataat tttttgtaat
     7441 tttgcagaac ttttcgggga tgttttggtt ttgacagcaa atcgaatcgg aaagtaagca
     7501 cgccgagagt cgtgatgcca tctcgttaat cacgggtcac aagccttaaa tggcgaaaac
     7561 aactttgcac tcgctgcgta attaagcata gctttttacg ctcaatcacc gccggccagg
     7621 tccggccgtg acgagtatcc cgcctgattt tgtgccgact atatcaggca atggggtatc
     7681 attaaagccg gctgcggaca tttactcctc taagatgtct taagattaag aggataagcc
     7741 ttgggtagcc tgtctgttgg cagccccagg atgaaaatca acaacagact aagcgtgtag
     7801 aaagcttttg gatggtttgt ctggacggcg gttcgagtcc gcccatctcc acaagctgtt
     7861 aattgtacaa tgattatgaa ataattattg taattgatcg cttaattaaa atttataatt
     7921 aatccaaaat gaagaaatta acattacttt catgcctgat gttcttgttt gcaggaacct
     7981 cagttgcaat tgcccaaaac gaagaacatt ccgcagaaat ttcaacttct gttcccgaac
     8041 tcgctaattt ccacaaaatt atcttcccaa tgtggcataa ggcatatcct gcaaaagata
     8101 ccgcagcttt aagaagcttt attcctgaaa ttaaggataa tatggcgaaa attaatgccg
     8161 ccaaattacc aggcatatta agagaaaaag agggactttg gaaaagtgaa cttgccaaat
     8221 tcaatacagt agctgaagat tattataagg ct
//
LOCUS       DAOTQL010000076         8222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7466_length_8222_cov_4.247582, whole genome shotgun sequence.
ACCESSION   DAOTQL010000076 DAOTQL010000000
VERSION     DAOTQL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_8222_cov_4.247582"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1029
                     /locus_tag="P5293_05510"
     CDS             121..1029
                     /locus_tag="P5293_05510"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HRR49392.1"
                     /translation="MREIVERIFHELQDFKDDAKVADYIPALSKVNPDLYAISVSTLG
                     KENISVGDYSARFSIQSISKVFALTLVIKTIGDEIWNYVGREPSGTAFNSLIQIELEE
                     GIPRNPFINAGALVVIDRLIDIYNDPYEKILSMVRELSGNSQIDFDSEVAYSEIENSH
                     RNLSLVHLMKGFGNIHNSPDKIIDLYSHCCSLSMSTEELSQSFLFLANCGINPFSEKV
                     VCSYRNAKRINALMLTCGLYNESGDFAYRVGLPGKSGVGGGIVAVMPGKLSVAVWSPK
                     LNLNGNSSLGIETLERFTTYLGTSVF"
     gene            1045..1722
                     /locus_tag="P5293_05515"
     CDS             1045..1722
                     /locus_tag="P5293_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRR49393.1"
                     /translation="MKDIINFLNDLNKNNNREWFESHKQEYKRIQNIFNNFVEKLIEG
                     ISVFDQSVKGQSVKSCTYRIYRDVRFTKDKSPYKPYMGAFISPKGKCGGYSGYYFHIE
                     AEGANYIGANILSVGIYKPEPIVLKSIREEIMLNGEEFMNTIALANDFILDQSSSLRK
                     IPAGYPKDNPYATYLRNKDYLLIKKIENKEIVGDQLLDFVLGSYKKAAPFNFWLNRAV
                     EYAYETF"
     gene            complement(1719..2678)
                     /locus_tag="P5293_05520"
     CDS             complement(1719..2678)
                     /locus_tag="P5293_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRR49394.1"
                     /translation="MDKPYVVGVDIGGQTTKTGIVDARGNVLAQNIIRTDKYTDVTPF
                     IDELCESVISLAKSIGGIQQIRGIGIGAPNGNYYTGTIENAVNIIWGGSNSIPFARMV
                     ADKIGIPVALTNDANAAAVGEMTYGAAKGMKDFIMITLGTGVGSGIVINGEVVYGHDG
                     FAGELGHTTSVRYNGRLCNCGRTGCLETYASAIGVARTAREWLEMSKEPSLLREFTEP
                     ITSKDVSEAASNGDELAIKVFEYTGQKLGEAFADFVAFSSPEAIILFGGLAKAGDLLL
                     NPIEKHMNANLLPLWKNKVKIIFSQLKESDAAILGASALGWEL"
     gene            complement(2703..>3349)
                     /locus_tag="P5293_05525"
     CDS             complement(2703..>3349)
                     /locus_tag="P5293_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49395.1"
                     /translation="SAFSFRHFVLGIIAIFIYVGVEVGIPNITNLFMTSDVSSGGLGI
                     DPVVAGTVVGTYWFLMLCGRLLGGTIGGIVSSKTMLVTTTIIGLILVLMAITMPLDIM
                     VNMPVFRSDISFGWALVPINIMFLILCGLCTSVMWGSIFNLATSGLGKYTAVASGIFM
                     MMVCGGGILPAIQGWLADVSGFINSYWIVFAGIAYMLFYALIGSKNVNKDIVVE"
     assembly_gap    3350..3359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3360..3996)
                     /locus_tag="P5293_05530"
     CDS             complement(<3360..3996)
                     /locus_tag="P5293_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49396.1"
                     /translation="MDNTKKKSYALPIAMMFSLFFIIAFVTGLQNPMGVIVKNQFGAS
                     NLLSQLGNLANFIAYAFMGIPAGLILQKIGYKKTALTAIVVGFIGVTITYFSGQLESF
                     SVYIIGAFISGLSMCMLNTVVNPLLNGLGANEKKGNQLIQFGGSINSIGATIVPVFIG
                     YLMGSDEASRTIAKANPALYLVMVIFVITFIVLYFVDIPQPFISKEKRSTRD"
     gene            4150..5100
                     /locus_tag="P5293_05535"
     CDS             4150..5100
                     /locus_tag="P5293_05535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR49397.1"
                     /translation="MKQRLSKRQKFILFFIIFILSNQLIVAQKYKFIDGWSKEINYRI
                     ERFLDSTKTLNERKVAVFDGDGTVFGQVPFYLADEALYQYADEQYKGKSDSLSKAKMK
                     ILKAMVEDGNNVGKPYVENRVHFLSGMTTEEVMEMGYQCYLRSYKGKFYPEMKILIDN
                     LKAYGFEVWILTASPEYLYQKFITIELGIPDINVIGVKSVIIDDRLTDIIVLPIPQDD
                     GKANTIPTFIKARPLLVGGNSRGDLDMINMSKGLKIVVNPDDVTVRGKDDGPMSGYTV
                     KQYWEKEGAVIVKCNDTADPMVRFHTREWGIRENLAHPKK"
     gene            complement(5090..5926)
                     /locus_tag="P5293_05540"
     CDS             complement(5090..5926)
                     /locus_tag="P5293_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRR49398.1"
                     /translation="MPKISSNNSQNNLNERHTCILEILRKQSSVSVSSLASQLNVSEV
                     TIRKDLSYLESLNLLYRAHGCAILINPYINDRHVNEKEKINTEKKRAIGIQAASLISD
                     NDSIIIASGTTVLSLAREIKNKNHLTVVTAAVNVSSILSRDKNIDVIQLGGILRNSSV
                     SVVGPFAERMMENFSCSKLFIGIDGFDIEFGLTTTNLMEANLNRIMMKAAQKIIVLAD
                     SSKFGLRGFSKICDITEIDQIITDCEAPKQIVEELNDMGIEVTLVDETQSNALSLRTP
                     IS"
     gene            6180..7745
                     /locus_tag="P5293_05545"
     CDS             6180..7745
                     /locus_tag="P5293_05545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRR49399.1"
                     /translation="MKREDLIKQISDESKVWDMVVIGGGATGLGVALDAVLRGFSVAL
                     FEMEDFAKCTSSRSTKLVHGGVRYLQKGDVQLVREALHERGRLKHNAPHLVKDQSFVI
                     PNYTYFDNFLYTCGLTFYDMLSWGLSLGRSLYISKRKVTKYLPTIVTNGLKGGVIYHD
                     GQFDDSRLAINLAQSICDEGGCPINRMKVEGINHNNEGKVSGVYVRDTLSGNKYSVKA
                     KSVINAAGVFVDDIILMDEPSKRRMVKPSQGIHLVFDKSFLKSDYAIMIPKTSDGRVL
                     FAVPWHEKVVVGTTDIVRDTPEIEPKPLDEEIDFIMNTAALYMTKKPTKSDILSVFAG
                     QRPLAAPKKEGKSTKEISRSHKIFVSDNDLITITGGKWTTYRKMAEDTVDRAISLNLL
                     PKSKCKTKNYKIHGYRENPDLSNHLYVYGSDMPAVEKLKNSGNGMNEKIHPKYDYSVA
                     EVVWAVREEMALTVDDVLGRRARLLFIDARAAIEAAPKVAQIMAKELGRSQEWINTQT
                     EDFINLANNYLVK"
     gene            7846..>8222
                     /locus_tag="P5293_05550"
     CDS             7846..>8222
                     /locus_tag="P5293_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49400.1"
                     /translation="MQIFKFFEPPKAKPLLPEEKIASEYKRMRLKVFLGAFLGYAAYY
                     LVRKNLALAIPGMIKPLEEGGLGYTKLELGIALSAIGIAYAFSKFIMGALSDRSDARK
                     FLVVGLILSSALMMSVGLFSFAT"
BASE COUNT         2731 a         1332 c         1545 g         2604 t
ORIGIN      
        1 tgctaaacca agtgggagag taggtagccg ccatcttttt aaaaagaggt gtccataaat
       61 tgttggacac ctcttttttt gtttaaattt gaagaataca aataattatt aactatagtt
      121 atgagagaaa tagttgagag gatttttcat gaactgcaag attttaagga tgatgccaaa
      181 gttgccgatt atattccagc tttatcgaaa gtaaatcctg atttgtatgc aatctcagta
      241 tcaacattgg ggaaagaaaa tatttctgta ggagattatt ctgcaaggtt ttcaattcag
      301 agtatttcta aagtatttgc acttacactg gtaattaaaa ctattggcga tgaaatctgg
      361 aattatgtag gtcgcgaacc ttcaggaact gcatttaatt ctttgataca gatagaattg
      421 gaagaaggaa ttccaagaaa tccatttatc aatgcaggag ctttggttgt gatagacaga
      481 ttaattgata tttataatga tccgtacgaa aaaatattaa gcatggttag agagttaagt
      541 ggtaactcac aaattgattt cgatagcgaa gtagcttatt ctgaaattga aaactctcac
      601 cgaaatttat cattggtgca tttgatgaaa ggatttggta atatccacaa tagtcccgat
      661 aaaataattg acttatactc tcactgttgt agtctctcaa tgtcaactga ggaattatcg
      721 caatcttttc tttttcttgc taattgtgga ataaatccat tttcggaaaa agttgtgtgc
      781 agctatagaa atgctaaaag aataaatgcc ttgatgctta cttgtggctt atataatgaa
      841 tcaggagatt ttgcctatcg tgttgggctg ccaggaaaaa gtggtgttgg tggaggcata
      901 gttgctgtta tgcctggcaa actttcagtt gcagtatggt ctcctaaatt gaatttaaat
      961 ggaaattcat cccttggaat tgaaacactt gaacgattta caacttattt gggaacttct
     1021 gttttctaat aatttaatag aattatgaag gatatcatta attttttgaa tgatctaaat
     1081 aagaacaata atagagagtg gtttgaatct cataaacagg agtataaacg cattcagaat
     1141 atttttaaca attttgtcga aaagctgata gaaggtattt ctgtgtttga tcaatctgta
     1201 aaaggacaga gtgttaagag ttgtacatat agaatataca gagatgttcg atttacaaaa
     1261 gataaatctc catataaacc ttatatgggt gcatttattt ccccgaaagg caaatgtgga
     1321 ggatattcag gatattattt tcatattgaa gcagaaggag caaattatat cggagcaaat
     1381 attctgtctg ttgggattta taaaccggag ccaatcgtat taaaaagcat tagagaggag
     1441 attatgctga atggggagga atttatgaac accatagcac ttgccaatga tttcattttg
     1501 gatcaaagct cttctcttag gaaaatacct gcaggttatc caaaagataa tccatatgcc
     1561 acatatctta ggaataaaga ctatctcttg attaaaaaaa ttgaaaataa agaaattgta
     1621 ggtgatcaat tacttgattt tgttttagga agctataaaa aagcagctcc gttcaatttt
     1681 tggctgaaca gagctgtgga atatgcttat gaaacattct aaagttccca gccaagtgca
     1741 ctggctccaa gtatggctgc atcagactct ttaagttgtg aaaaaataat tttaacttta
     1801 tttttccaca gtggtaataa gttggcattc atgtgtttct caatagggtt gagaagcaaa
     1861 tctccggctt tagcaagtcc accgaagaga attatcgcct ccggtgagct aaatgccaca
     1921 aaatcagcaa atgcttctcc taatttttgt ccagtgtatt caaacacttt tattgcaagt
     1981 tcatcaccat tgcttgcagc ttcagaaacg tcttttgagg ttattggttc tgtgaattct
     2041 ctcaaaagac ttggttcctt actcatttca agccattctc tggcagttct tgcaacgcct
     2101 attgcggatg cataggtttc aagacatcct gttctgccac aattacaaag tcttccattg
     2161 tatcttacag aggttgtatg tcctaattct cccgcaaagc catcatgccc atatacgact
     2221 tccccattaa ttacaatgcc acttccaact cctgtgccaa gtgttatcat aataaaatcc
     2281 ttcattcctt tagctgcacc ataagtcatt tctcctacag ctgctgcatt tgcatcatta
     2341 gtcaatgcaa caggaatccc aattttatct gcaaccattc tggcaaaagg tatggaattg
     2401 gaccctcccc aaattatatt aacggcattt tcgatagttc cggtgtaata attgccattt
     2461 ggtgcaccta ttccaattcc tctgatctgt tgaattcctc caattgattt ggcaagagag
     2521 ataactgatt cacaaagttc gtcgataaaa ggagtgacat ctgtgtattt atctgtacgg
     2581 ataatgtttt gagccaaaac attacctcta gcatcaacta tccctgtttt agttgtttgt
     2641 cctcctatat caactcctac tacataaggt ttatccatga taattatgtt aaggttaatc
     2701 aattattcta caacaatatc tttgttgaca ttttttgaac ctatcaaagc atagaacagc
     2761 atgtaagcaa ttccggcaaa aacaatccag taactgttaa taaatcctga aacatcagca
     2821 agccatcctt gaattgctgg taaaattcct cctccgcaaa ccatcatcat aaaaatacct
     2881 gaagctactg ctgtgtattt cccaagccct gaagtggcta aattaaatat actgccccac
     2941 ataactgatg tgcacaaacc acataaaatc aaaaacatga tattaatagg aacaagagcc
     3001 catccaaaag atatatctga tctgaaaaca ggcatattta ccataatatc gagtggcatg
     3061 gttatagcca tgagaacgag aatcaagcca ataatagttg tagtgacaag cattgttttg
     3121 cttgaaacta tgcctccgat tgttcctccg aggaggcggc cgcacagcat tagaaaccag
     3181 tatgtaccaa caactgttcc ggcaacaaca ggatctattc ctaatcctcc tgatgaaaca
     3241 tctgaggtca taaaaagatt agtaatattt ggaataccaa cttccactcc aacatatatg
     3301 aatattgcaa ttattcccaa cacaaaatga cggaatgaaa atgcactgtn nnnnnnnnna
     3361 atcccttgtg cttctttttt cttttgaaat gaatggttgt ggtatatcaa caaagtatag
     3421 gacaataaat gtaattacga aaatgaccat tacaagatac aaggcaggat ttgcttttgc
     3481 aattgttctg gaagcttcgt cagatcccat caggtatcct ataaaaacag gaacaattgt
     3541 ggcaccgatt gagtttatcg atcctccaaa ctgaattagt tgattccctt ttttctcatt
     3601 agctcccaat ccgttcaaca atgggtttac aacagtattc aacatgcaca ttgacagtcc
     3661 ggaaataaaa gcacctataa tatatacact aaaactttct agctgccctg aaaaataagt
     3721 aattgtcaca cctatgaaac ctacaactat tgccgttaag gctgtttttt tataacctat
     3781 tttttgcaat ataagtccag caggtatgcc cataaaggca taagcaataa aattggccaa
     3841 atttccaagt tgtgagagaa gattactagc tccaaattga ttcttgacta taacgcccat
     3901 gggattttgc aatcctgtga cgaaagcaat aatgaaaaat aatgaaaaca tcattgcaat
     3961 cggcaatgcg taactttttt tctttgtgtt gtccatcaat gtatatagtt taagtattat
     4021 aatgtcaaag ataataaatt gcatttattt tttgaatttt gtttagctaa aagaatgttt
     4081 cgttttttta ataaaaatgt agcgaaacga atcaaaaatc gtatttttgc attaaataca
     4141 gattgagtta tgaaacaaag actttcgaaa agacaaaaat ttattttatt ttttatcatt
     4201 tttattttat ccaatcaatt gatagtcgct caaaagtata agtttatcga tggttggtct
     4261 aaagaaatta attatagaat tgaaaggttt ctcgattcta caaaaacatt aaatgaaaga
     4321 aaagtggcag tatttgacgg agatggaact gtatttggtc aagtaccgtt ttatctcgca
     4381 gacgaagcct tatatcaata tgcagatgaa caatataaag gtaaaagtga ctctttgtca
     4441 aaagctaaga tgaaaattct taaggcaatg gtcgaagacg gcaacaatgt tggaaaacct
     4501 tatgtagaaa acagagtgca ttttctttca ggcatgacca ccgaagaggt aatggaaatg
     4561 ggatatcaat gttatttaag atcatacaaa ggaaagtttt atcctgaaat gaaaatcctt
     4621 attgataatt taaaagctta tggatttgaa gtatggattc ttaccgcatc gcctgaatac
     4681 ctttatcaaa agtttatcac aattgaactt gggatacctg acattaacgt tattggagta
     4741 aaatccgtaa ttattgatga tagacttact gatataatag tattgcctat tcctcaggac
     4801 gacggtaagg ctaatacaat tcctactttc ataaaagctc gtccattgct ggttggagga
     4861 aatagcagag gagatttgga catgattaac atgtcaaaag gtttgaagat cgtagtcaac
     4921 cctgatgatg tgacagtcag aggaaaagat gatgggccga tgagtggcta tactgtgaaa
     4981 caatattggg aaaaagaggg tgcagttatt gtaaaatgta atgatacagc agatccaatg
     5041 gttcgtttcc acaccagaga gtggggaata cgggaaaacc tggctcatcc taagaaatag
     5101 gagttctaag acttaaggca ttggattgag tttcatcgac aagtgttacc tcaattccca
     5161 tatcattcag ttcttcaact atttgcttag gagcttcaca gtcagttata atttgatcaa
     5221 tctctgttat atcacatatt ttactgaatc ctctaagtcc gaatttagac gaatctgcta
     5281 atacaattat tttctgtgca gctttcatca ttatcctgtt aaggttagct tccataagat
     5341 ttgttgttgt tagaccaaac tcaatgtcga atccgtcaat accaatgaag agtttgctac
     5401 atgaaaaatt ttccatcatt ctttctgcaa aagggcctac aacagaaacg gaactgtttc
     5461 ttaatatacc tccaagttga atgacatcta tgtttttatc tcttgaaagt atggaagaaa
     5521 cgtttactgc agcagtgact actgtaagat gatttttatt tttgatttca cgtgcaagag
     5581 aaaggacagt ggttccggaa gctataatta ttgaatcatt gtctgagata agggatgcgg
     5641 cttgaattcc tattgccctt ttcttttctg tattgatctt ttctttttcg ttaacatgtc
     5701 tgtcattgat gtatggatta attagaattg cgcaaccatg ggcacggtaa agaaggttga
     5761 gggattcaag ataacttaag tcttttctaa tagtgacttc cgaaacattc aactgacttg
     5821 caagagaaga tactgacacc gaactctgtt ttctcaaaat ttcaagaata caggtatgtc
     5881 tttcattaag attattttga gaattatttg aagatatctt tggcataatt tcgtaatatg
     5941 ttttttaaac ttttacgaat atacgaaatg tttaaattta ttaataatta taaccatgaa
     6001 accttaaaaa atatatttat catttatgaa agcaataatt gcatttttta ataaaattta
     6061 gtttttgaaa gtaaaaatat gcgtaaaatt ttgataatat aaaattatct acttaatttt
     6121 gtttcgaaac aaaacagtat gcttttcgaa taggtaagtt gtgtaaaaat ttaatataaa
     6181 tgaaaaggga agatttaatt aaacagatat ccgacgagtc aaaagtatgg gatatggttg
     6241 taattggagg gggggcgaca ggacttggtg ttgctcttga cgcagtattg agagggttta
     6301 gtgttgcact ttttgaaatg gaggattttg ctaaatgcac ctcaagtaga agcacaaaac
     6361 ttgtgcacgg aggagtgagg tatttacaaa agggagatgt acaacttgtg agagaagctt
     6421 tacatgaaag aggcaggttg aagcataatg ctccacacct tgtcaaggac caatcatttg
     6481 taataccaaa ttatacttac tttgacaatt ttctttacac ttgtggtctg acattctatg
     6541 acatgctttc ttgggggctc agtcttggac gttctttata tatatcaaaa agaaaggtga
     6601 caaaatattt acccacaata gtaacaaatg gacttaaagg tggcgtgata tatcatgatg
     6661 gtcagtttga tgattcacga cttgcaataa atcttgctca atctatttgt gatgaaggag
     6721 ggtgtcctat aaacaggatg aaggttgaag gtataaatca caacaatgaa ggaaaggttt
     6781 caggtgttta tgtacgtgat actctttcag ggaataaata ttctgtaaaa gcaaaatcgg
     6841 taattaatgc tgccggtgtt tttgtagatg atatcatttt gatggatgag ccttcaaaac
     6901 gtagaatggt aaaaccaagt caaggcattc atcttgtttt tgataaatct tttttgaaga
     6961 gcgactatgc tataatgatt ccaaaaacat ctgatggtcg agtgcttttt gctgttccat
     7021 ggcatgaaaa ggttgtagta ggaacaacag atatagttag agacactcct gaaatagagc
     7081 ccaaaccact cgatgaggag atagatttta ttatgaacac agctgctcta tatatgacaa
     7141 aaaagcctac caaaagtgac atcctttctg tatttgcagg gcaacgtcct ttggctgcac
     7201 caaagaaaga gggaaagagt accaaggaga tttcaagaag tcacaaaata tttgtgtcag
     7261 acaatgactt gataacaatt accggaggaa agtggacgac ttacagaaaa atggcagaag
     7321 acactgtaga tagggcaatc tcacttaatc ttcttccaaa gagtaagtgt aaaacaaaaa
     7381 attataaaat tcatggatat agagaaaatc cagatctctc aaatcatctc tatgtttatg
     7441 gttcagacat gcctgcagtt gaaaaactga aaaattcggg gaacggtatg aatgaaaaga
     7501 ttcacccaaa gtacgattac tcagttgctg aagtcgtttg ggcggttaga gaggaaatgg
     7561 ctttaacagt tgatgatgtc ttgggtcgta gggcaagact tctgtttatt gatgctagag
     7621 cagctataga agctgcgcca aaagtcgcac agatcatggc aaaggaactt ggtcgcagtc
     7681 aagaatggat aaatactcaa actgaggatt ttataaatct cgctaataat tatttggtta
     7741 agtaaatgtt acatgaacgg tattaaaacg aaaaatgaaa gagggaaatt gtttactttg
     7801 cgtaataaat aacttacgaa aaaacaaaat atagatttaa taatcatgca aatatttaaa
     7861 ttttttgaac ctccgaaagc aaagcctctt ttacctgaag agaaaattgc ctctgagtat
     7921 aaaagaatga ggttaaaggt ttttcttggt gcttttttgg ggtatgctgc ttattattta
     7981 gtcagaaaaa atctggcgct tgccatacct ggaatgataa aacctctcga agaaggtgga
     8041 cttggatata ctaaactgga attgggtata gctctttctg caattggaat tgcatatgca
     8101 ttcagtaagt ttataatggg cgctttgtca gatagatcgg atgctcgaaa atttttggtt
     8161 gttggattaa ttctttcttc ggcacttatg atgtctgtgg gattattctc gtttgcaaca
     8221 ag
//
LOCUS       DAOTQL010000077         8220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7468_length_8220_cov_4.088059, whole genome shotgun sequence.
ACCESSION   DAOTQL010000077 DAOTQL010000000
VERSION     DAOTQL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7468_length_8220_cov_4.088059"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..1295
                     /locus_tag="P5293_05555"
     CDS             30..1295
                     /locus_tag="P5293_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR49401.1"
                     /translation="MNTGLRIFLTLIFFQILLSGEIQAQYTIDICQKKAAENYPLIRQ
                     YGLIESSKMYDLKNAGMAYLPQVAFQAKASYQSDVTEIPLNIPGINIESLSKDQYNTA
                     VQVDQLVWDGGITSLRKSAIRQQSELERSKVDVDMYAIKERVSQTYFGIVLIDKQIEQ
                     LNLLLKDLDRNLNRIKSYMDNGLANESDIDQINVEIINAGQKMCELNAGRKAYISVLS
                     SMMGVEPDFKATFEVPQHLTVSKEIKRPELSVMAYQSKLLDTQAGMVRSQLRPRFGLY
                     FQAGYGRPGLNMLENQFSPFYIAGVKAVWSLGGLYTKRNELSMIEINKKRIENTRDAF
                     IYNTNLQVIIQSEEIDKIAELLRRDDEIISLRERILTAAEIKQSEGIISVSELMHEMN
                     QLDGARVTRARHEVELLMAKYNLKITTNN"
     gene            1312..2226
                     /locus_tag="P5293_05560"
     CDS             1312..2226
                     /locus_tag="P5293_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR49402.1"
                     /translation="MNWIKKCVLISLPLSIAVSCINSNDSFDASGNFEAIEVIVSSEA
                     TGRIQEFNIDEGSQVLTGQQVGCVDSVQLCLQKERLLANIAAINNRKLDIPTQLAAFD
                     QQIDYLEKERLRAERLVAAGVGNTKTLDDINSQLRTLQKQREAQLSSMKNGNISLENE
                     ISGLMAQLKQLNDQIGRCRIASPITGTVIGKYIEKGEITVTGKPLFKVANLDTMILKA
                     YITQNQLTKLKLNDSVRVFTDYDDGARKEYGGKIIWISDEAEFTPKTIQTKEERKNFV
                     YAIKVQVKNDGYLKIGMYGELEFLNKVD"
     gene            2226..3146
                     /locus_tag="P5293_05565"
     CDS             2226..3146
                     /locus_tag="P5293_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR49403.1"
                     /translation="MESVVVDGVKKCYGQVVALKGVSFSAHKGEIFGLIGPDGSGKST
                     LFKIMTTLLLPDSGTVEVAGYDVLKDYTIIRNIIGYMPSKFSLYKDLSVEENMKFFAT
                     LFKTTIEENYYLVKDIYSQIEPFKKRKAKNLSGGMKQKLALSCALIHKPSVLFLDEPT
                     TGVDPVSRKDLWTMLKKLSEQEITIIVSTPYMDEAKLCDRIALIKEGEFMKIDAPDGI
                     VNSLHKKVMAVKGENMYLILNVLRTLPVVEMAFAFGETNHITLKDSASDLVPVDIVRM
                     LEDKGFHNVDVKEIRAGVEDCYMVLSKENI"
     gene            3161..3919
                     /locus_tag="P5293_05570"
     CDS             3161..3919
                     /locus_tag="P5293_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR49404.1"
                     /translation="MPKETNREKIIEVVELTKKFGNFTAVDHISFEVYKGEIFGFLGA
                     NGAGKTTAMKMLCALSIPTSGRASVAGYDIMKRSNMVKANIGYMSQRFALYEDLKVWE
                     NFRLFGGIYGMSEEAIVNQSDFMLEKLGFMSVKDSLVKSLPLGWKQKLAFSVATFHKP
                     KVVFLDEPTGGVDPSTRRQFWELIYQSANEGTTIFVTTHYMDEAEYCDRVSMMVDGRI
                     EALDTPAALKEKNEVSSMEGVFLKLARKAKRTEI"
     gene            3924..5054
                     /locus_tag="P5293_05575"
     CDS             3924..5054
                     /locus_tag="P5293_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR49405.1"
                     /translation="MKQFLIFVNKEFLHIFRDPRTILILLIIPAIQIILFGFALTSDI
                     KNIDIAVFDQSHDASSRLIIPKLLNSPYFNVVASVDNMDEADKLLQSGETMAVIIFEK
                     GLESDIMREGAGNVQIITDGSDPNTSNAVSRYLQAIILTAQKELIKIAPLQQMSLTND
                     RITIMPEPNMLYNPLIKSSYSFVPGVMGLILMLICSMMTSISIVREKEEGTMDVLLVS
                     PMKPLNIILAKSVPYLALSLINLATILLLSVFLLGVPITGSLFWLLIISIIFIFVALA
                     TGLLVSSLVSTQVAAMLMSGLVFMVPVMLLSGMIFPIDNMPFILRAIAQFIPAKWYIV
                     AVKKLMIQGLPVSYVLTEMLILFAMAISFIFISLKAFKMRVS"
     gene            5056..6147
                     /locus_tag="P5293_05580"
     CDS             5056..6147
                     /locus_tag="P5293_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR49406.1"
                     /translation="MLKYLIEKEFKVFVRNRFLPKLIFIFPCCHLLLMPWAANLDIKN
                     LKVAVVDEDLSSISSKLVTKIDASKYFMIECCCESYKEAKYLVDNGDIDIVIEIPQNF
                     EKNIIKEGSDKVHLAINAVDAMRGSLGSLYLTEILSDFAVQQSISGDVSIINISTQTR
                     YNPTMDYNKFMVPALIVMILTLLTGFLPALNIVSEKEIGTIEQINVTPVSKFTFVLSK
                     LIPYWIIGFIILTILVILARVVYNIYPVGSILTFYLMASIFVIVMSGMGLVISNHSDT
                     LQQAMFVMFFFVHIMILLAGLFTPVDSMPVWAQMIAAVNPMRYFIEIMRMIYLKGSSM
                     LDFKWQIVILFGFALFFNSWAIVSYRKKK"
     gene            6188..6796
                     /locus_tag="P5293_05585"
     CDS             6188..6796
                     /locus_tag="P5293_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49407.1"
                     /translation="MKKLTFLTLLLMFMSNNYAGAQAPPKINFYGVDFSMVKVFGAKE
                     TPDQFVKAFEEINRAVIKDSKKFPFTNFFIKYAHELDNSAELKRQRFDSYSNVNIDEA
                     IYRSRVMDGSSMSTYNKQFKLSDQDIASLIKSFNTGKDTGYGVLYVAEMLNKQDAVGR
                     FVVVCFNAKTKRILFTDRVEGKAKGRGLGNYWAASLSDIMKI"
     gene            complement(6801..>8220)
                     /locus_tag="P5293_05590"
     CDS             complement(6801..>8220)
                     /locus_tag="P5293_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HRR49408.1"
                     /translation="VTNTSSGKISVKEYSQTNSNILDNYGEIAAEKTFTTSSGTQINN
                     YCHITANAVSFYGTTVNMFSGSLFHCQSLTPNNTSLNMNGGSIFLVDGNVSDVYNLSL
                     NNTSSTFSLFKCLGNFTNGMTSSVGAGTCSVSGKIDFVLAPLVNGSGINGIDRFAPVF
                     SNGALLSNTQTQNIVATVCNLGLGQISGGGSSDHDGDGIPEGTDFDDNDPDVAFVSYF
                     PAEGTWGTIAFEDLWPEKGDYDVNDLVMDFRICTYSNASNLVTKLKIDYNVRASGSTY
                     VLGCAFQLDQVLASQVQSVTGQTLGSGNFFTTASNGAEAAVNLAVIPLFNSQRDLLSY
                     SGFLNTDTNIAFINTPDYAVTVKFLEGLSQSKFSVSKINFFITANSRGREIHLPGYTY
                     TTKFLTSLTNGASLYPGDVFKFIDGMMWGLMIPQTFNYPVERAAIYNAYTHFAEWATS
                     GGTSFADWYSLQNGYTNESLIY"
BASE COUNT         2646 a         1312 c         1707 g         2555 t
ORIGIN      
        1 attatgcatc ttaacgggag gaataaataa tgaataccgg tttaagaatt tttctgactc
       61 tgatcttttt ccagatatta ctgtcaggcg aaatacaagc tcagtataca attgatattt
      121 gccagaaaaa ggcagctgaa aattaccctt taatcaggca atacggcctt attgagagtt
      181 caaagatgta tgacttgaaa aatgccggta tggcatatct gccccaggta gcttttcagg
      241 cgaaggcttc ctatcaatct gatgtcacag aaataccttt gaatattcca gggataaata
      301 ttgagtctct aagtaaggat cagtataata ctgccgtgca ggttgatcag ttggtttggg
      361 acggaggtat aacttctctt agaaaatctg caatcaggca gcagtctgag ttggaaagat
      421 ctaaagttga tgtcgacatg tatgccataa aagagagagt gagtcaaact tattttggta
      481 tagttttaat tgataaacag atagaacaac taaatctttt gctgaaggat cttgacagga
      541 atctcaatag aattaaatct tatatggaca acggcttggc aaatgaatct gatattgacc
      601 agataaatgt tgaaataata aacgccggtc agaaaatgtg tgagcttaat gcagggcgga
      661 aagcatatat ctcagtattg tcgtctatga tgggggttga gccggacttt aaagcaactt
      721 tcgaagtgcc acaacattta acagtttcaa aagagataaa aagaccagaa ctatcagtaa
      781 tggcttatca aagtaaattg ctcgatacac aggcaggaat ggtcagatca cagcttagac
      841 ctcgatttgg tctctatttt caggcaggat atggacgtcc gggacttaat atgttggaaa
      901 atcagttttc tccattttat atagcaggtg ttaaggctgt atggagttta ggcgggcttt
      961 atacaaaaag aaacgaacta agtatgatag aaataaataa aaaaagaatt gagaacacaa
     1021 gggacgcttt tatttataat actaatcttc aagtaattat tcaatccgaa gaaatcgata
     1081 agatagccga attgcttagg agggacgacg aaattatttc gttgcgagag agaattctca
     1141 ctgctgctga gataaaacag agtgaaggca ttatctctgt ttcggaactt atgcatgaga
     1201 tgaatcagtt ggatggagca agagttacca gagcaagaca tgaagtggag cttttgatgg
     1261 ccaaatacaa tcttaaaatt actaccaata attaattaaa cagttaagag tatgaattgg
     1321 attaaaaaat gtgtattgat ttccttgcct ttgagtattg cagtctcatg tataaatagt
     1381 aatgactctt ttgatgcttc cggcaatttt gaggcaattg aagtaatagt ctcatcagag
     1441 gcaacaggga ggatacagga gtttaatatc gatgaaggtt cacaagtctt aactggccaa
     1501 caggtagggt gtgtggatag tgtacaactt tgtcttcaga aagagagact tttggcaaat
     1561 atagctgcta taaacaatag aaaattagat atacctacac aattagcagc ctttgaccag
     1621 cagatagatt atcttgaaaa ggaaagactg cgggctgaaa ggctggtagc agcgggtgtg
     1681 ggcaatacta aaactttgga tgacataaac agtcagcttc gaactcttca gaaacaaaga
     1741 gaggcacaac tatcttcaat gaaaaatggg aatatcagcc tggaaaatga aatctccggc
     1801 ttaatggctc agcttaaaca attaaacgat cagataggaa gatgcagaat agcgtctcca
     1861 attacaggta cagtgattgg caaatacata gaaaaaggtg aaataaccgt caccggaaaa
     1921 ccgcttttta aagttgcaaa tctggataca atgattctta aggcatatat tactcaaaac
     1981 cagctgacaa aattaaaatt gaatgattct gttagagtct ttacagatta tgatgatgga
     2041 gcgagaaaag aatacggagg taaaattata tggatatcag atgaagccga atttacgcct
     2101 aagacaatac agacaaagga ggaaagaaaa aactttgtat atgcaataaa agttcaggta
     2161 aagaacgatg gctatctgaa gataggaatg tatggagaat tggagttttt aaacaaagta
     2221 gactgatgga gtcagtggta gttgatggtg taaaaaaatg ctatggacag gtagtcgctc
     2281 ttaagggggt tagtttctcc gcacacaagg gagagatctt tggactaata ggacctgacg
     2341 gatctggtaa gagtacactt tttaaaatta tgactacact gttactccct gattcaggca
     2401 ccgttgaagt tgccgggtat gatgttttga aagactatac tataataaga aacataattg
     2461 gttacatgcc aagtaagttt tctctttata aggatctctc tgttgaagaa aacatgaaat
     2521 tctttgcgac tttatttaaa accacgattg aagagaacta ttatcttgtt aaggatattt
     2581 atagccagat tgaacctttc aaaaaaagga aggccaaaaa tctttcaggc ggcatgaaac
     2641 aaaagcttgc attaagttgt gctctgattc acaagccgtc cgtattgttt cttgatgaac
     2701 cgacaaccgg cgttgatcct gtttcaagga aagatttatg gacaatgtta aagaaactca
     2761 gtgaacagga gattacaatt attgtttcca cgccttacat ggatgaagct aaactttgtg
     2821 acagaattgc acttatcaaa gaaggggagt ttatgaagat tgatgctcct gacggaattg
     2881 taaactcact tcacaaaaaa gtaatggctg tcaaaggtga gaatatgtat cttattctta
     2941 atgtcttgag aacattgcct gttgtggaaa tggctttcgc ctttggggaa actaatcata
     3001 taacattgaa agattctgca tcggacctgg tacctgttga tattgtcaga atgctcgaag
     3061 ataaaggctt tcacaatgtt gatgttaagg agataagagc tggtgtggaa gattgctata
     3121 tggtactgtc aaaagagaac atataaatta ttattaagtg ttgcctaaag agacaaatag
     3181 agagaaaatc attgaagttg ttgagcttac aaaaaagttt ggcaatttta cagctgttga
     3241 tcacatctcc tttgaagtat ataaaggaga aattttcggt tttttggggg caaacggggc
     3301 aggcaaaact acagcaatga aaatgctgtg tgctttaagt ataccgactt ccggaagagc
     3361 aagtgttgca ggttacgata tcatgaagag gtccaacatg gtcaaggcca acataggtta
     3421 tatgagtcaa cgctttgcct tgtatgaaga ccttaaggtt tgggagaact tcagactgtt
     3481 cggaggaata tatgggatga gtgaagaagc aattgttaat caaagtgatt ttatgcttga
     3541 gaaattaggt tttatgtcag taaaggattc actagtcaaa tctcttcctc ttgggtggaa
     3601 acagaaactt gcattctcag ttgcgacatt tcataaaccc aaagttgtgt ttttggatga
     3661 gcctactggc ggggtggatc catctacccg tagacagttt tgggaattaa tttatcaatc
     3721 tgccaatgag ggcactacaa tttttgtgac gacgcattat atggatgagg ctgaatattg
     3781 tgacagggta tcaatgatgg ttgatggaag aattgaagca cttgatactc cggcagctct
     3841 taaggagaag aatgaggtaa gtagtatgga aggagtattt ctcaaacttg caaggaaggc
     3901 aaaaaggaca gaaatttaat aagatgaagc aatttttgat ttttgttaat aaagaatttc
     3961 tgcatatttt cagagatccg agaacaattt taattttgtt gatcataccg gcaatccaaa
     4021 taattttgtt tggttttgct ttgacgagtg atataaaaaa tatagatata gcggtttttg
     4081 accagtcaca tgatgcttca tcaagactca ttattcctaa actcttgaac agtccatatt
     4141 ttaatgttgt agcttcagta gataacatgg atgaagccga caagcttttg caatcaggtg
     4201 aaaccatggc agtcattatt tttgaaaaag gacttgaatc ggatatcatg cgtgaaggag
     4261 cgggcaatgt gcaaataata acagacggtt cagatccaaa cacctcaaat gctgtttcaa
     4321 gatatcttca ggctattatt ttgactgccc agaaagaatt aataaagata gcaccattgc
     4381 agcaaatgtc tttaaccaat gacagaataa ccattatgcc ggaacctaac atgttgtata
     4441 atccgttaat aaagagttca tatagttttg tgccaggagt aatgggattg attctcatgc
     4501 tgatttgttc gatgatgaca tcaatatcga tagttagaga gaaagaagag ggtactatgg
     4561 atgttttact tgtttctccg atgaagcctc taaacatcat tttggccaag tctgttccat
     4621 atctggctct ttcacttatc aatcttgcca ctattttatt actgtcagtc tttcttcttg
     4681 gggtcccaat aacgggaagt cttttttggc ttttgataat atcaattatt tttatatttg
     4741 ttgctcttgc tacagggctt ctggtatcat cgcttgtaag cacgcaagtg gcagctatgt
     4801 taatgtcggg tttggtattt atggtccctg taatgttgct ttccggcatg attttcccca
     4861 ttgataacat gccatttata ctaagagcca tcgctcaatt tattcctgca aagtggtaca
     4921 ttgttgcggt taagaagttg atgattcagg gacttcctgt cagttatgta ttgacggaga
     4981 tgctaattct ttttgctatg gcaatatcct ttattttcat cagtttgaaa gcattcaaaa
     5041 tgagagtttc ataatatgtt gaaatacttg atagagaagg agtttaaggt ttttgtaaga
     5101 aacaggtttc ttccaaagct cattttcata tttccatgtt gtcatttatt actcatgcct
     5161 tgggctgcaa accttgatat aaaaaatttg aaagttgctg ttgttgatga agatttaagt
     5221 agtatttcct caaagcttgt tacaaaaatt gatgcctcca aatattttat gattgaatgt
     5281 tgctgtgaat catacaagga ggcaaaatat cttgtggata atggagatat agatattgtt
     5341 attgaaatac ctcaaaattt tgaaaaaaac attattaagg aaggatcaga taaagttcat
     5401 cttgctataa atgctgttga tgcaatgcga ggttctcttg gaagtttgta cttaacagag
     5461 attttgtctg attttgcagt tcagcaaagt atttcaggcg atgtatcaat tattaatata
     5521 tctactcaga ccagatataa tcccactatg gactataaca aatttatggt gcctgcactt
     5581 attgttatga ttcttacttt attaaccgga tttctgcctg cattgaacat cgtttctgaa
     5641 aaggagatag gaacaattga acaaataaat gttactcctg tttctaaatt tactttcgta
     5701 ctttctaaac ttattccgta ttggattata ggatttatta ttcttaccat acttgtaatt
     5761 ttagctcggg tcgtttataa tatttatcct gtcgggtcta ttttgacttt ttatttaatg
     5821 gcatcaatat ttgtaattgt catgtcgggg atggggctag ttatatccaa tcattcagat
     5881 actttacagc aggcaatgtt tgtaatgttt ttctttgttc atattatgat tttactcgca
     5941 ggtctgttta cacctgtaga tagtatgcct gtttgggctc agatgattgc tgcagttaat
     6001 ccaatgagat attttattga gatcatgagg atgatatatt taaagggtag cagtatgtta
     6061 gattttaaat ggcagatagt tatccttttt ggatttgcac tcttttttaa ttcttgggca
     6121 atagtgagct atagaaaaaa gaaataaaat ttttttataa ctttgttaaa acctaaaaag
     6181 ccaagcgatg aaaaaattaa cttttttaac gttattactc atgtttatga gcaataatta
     6241 tgcaggagca caggctccgc ctaaaattaa cttttacggt gtagattttt ccatggtaaa
     6301 agtgtttggt gcaaaagaga ccccggacca gtttgtcaaa gcttttgagg agattaacag
     6361 ggctgttatt aaggattcca aaaagttccc tttcacaaat ttttttataa aatatgcaca
     6421 tgagttggat aactcagcgg aactaaaaag gcaaaggttt gacagttaca gcaatgtaaa
     6481 tattgatgag gccatttaca gaagccgcgt aatggacggg agcagcatga gtacttataa
     6541 caagcagttt aaactttctg atcaagacat agcttccctt attaaatctt ttaatactgg
     6601 caaagatacc ggatatggtg ttctttatgt tgccgagatg cttaacaagc aggatgctgt
     6661 aggcagattt gttgtagttt gttttaacgc caaaacaaag agaattcttt ttaccgacag
     6721 ggtagaaggc aaagcaaaag gacgcggatt aggcaattat tgggcggctt ctttgtccga
     6781 tatcatgaag atatgatact ttaatatatc aagctttcat tagtataacc gttttgcaga
     6841 gagtaccaat ctgcaaagga agtgcctcct gatgtggccc attcggcaaa atgagtgtaa
     6901 gcattataaa tagcagctct ctcgacagga tagttgaaag tttgaggaat cattaaaccc
     6961 cacatcatgc cgtcgataaa tttaaataca tctccgggat atagcgatgc tccatttgta
     7021 agagatgtga ggaacttggt tgtataggtg taacctggaa gatgaatttc tcttcctctt
     7081 gaattggctg ttatgaagaa attaatctta cttactgaaa atttggattg agatagacct
     7141 tctaaaaatt ttactgtgac agcataatct ggtgtgttaa tgaaagcaat atttgtatcg
     7201 gtatttaaga agccactata acttaagagg tctctctggc tgttaaataa agggataacc
     7261 gcaagattta cagctgcctc tgcaccatta gatgctgttg tgaagaaatt gccggatcct
     7321 aaagtttgtc ctgtaacaga ttgaacctgt gaagccaata cctggtcaag ctggaatgcg
     7381 caccctaaga catatgtact tccacttgct cgaacattat agtcaatttt aagttttgtc
     7441 accagatttg aggcatttga gtatgtacat attcggaaat ccatcacaag atcattaacg
     7501 tcataatctc ctttttcagg ccacaaatct tcaaaagcaa tcgtccccca tgtaccctcg
     7561 gccggaaagt aagatacaaa agccacatct ggatcattat catcaaagtc tgtgccttca
     7621 ggtattccat caccatcatg atctgatgac cctcctccgg aaatttgtcc aaggcctaaa
     7681 ttacaaaccg tagcaactat gttttgagtt tgtgtatttg aaagcaatgc accatttgaa
     7741 aatacaggcg caaatctgtc tattccattt attcctgagc catttacaag aggggcaagc
     7801 acaaaatcaa tcttgccgct tacactgcaa gttcctgccc caacagacga agtcataccg
     7861 ttagtaaaat tcccgagaca tttgaaaagt gaaaaagttg atgaagtatt attcaatgaa
     7921 agattataaa cgtctgatac attcccgtca actaaaaaga tgcttccccc attcatattt
     7981 aatgatgtgt tgtttggtgt tagagattgg cagtggaaaa gagaaccact aaacatgttc
     8041 acagttgttc cataaaaaga taccgcattc gcagttatgt gacaataatt attgatttga
     8101 gtaccggagg atgttgtaaa agttttttct gctgctatct ctccatagtt atctaaaata
     8161 ttactgttgg tttgtgagta ttccttgact gagatctttc cactggaggt gttggtaaca
//
LOCUS       DAOTQL010000078         8134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7559_length_8134_cov_4.783513, whole genome shotgun sequence.
ACCESSION   DAOTQL010000078 DAOTQL010000000
VERSION     DAOTQL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7559_length_8134_cov_4.783513"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..402)
                     /locus_tag="P5293_05595"
     CDS             complement(37..402)
                     /locus_tag="P5293_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRR49409.1"
                     /translation="MQKLTAREEEIMSHFWEKGALFVRELIDFYADKKPHFNTLSTIV
                     RGLEEKGFLSHHSFGNTYQYYPLVSRDDYKKGALDNVVKKYFKSSYLGVVSSLIEEEE
                     VSLEELKKLISQVEGSSKK"
     gene            complement(538..1668)
                     /locus_tag="P5293_05600"
     CDS             complement(538..1668)
                     /locus_tag="P5293_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR49410.1"
                     /translation="MKKSFLTLVLLMFFGIISAQEQSKRGPFLTNGFWDNWFISAGVG
                     GNVYFGENDSELSFGDRISTALDFSVGKWITPCSGFRFQYAGIDAKGYGPNALYADNN
                     GDESFNVLNLHADYILNLSNFVGGYKQTRFWQFVPFVGVGWAKSWKEDVNPEYNEVGV
                     SGGLINKIRLSDALDANLELRGLFVHQRFDGTVGGFKGEGMASATIGLTYKFNRRDFT
                     KYVEPVPCDYTPYTNKISDLEKKIADADAEASRLAEELNAEKNKAPEVVTVKEYLMGK
                     MSVWFVINSAKISTTDMINIGNYAEVMKQSGKTYKITGYADKETGNPKINQRLSEQRA
                     KNVFDALVNKFGVNPSQLEVIAKGDREEPYNRPVLNRVVIIE"
     gene            complement(1780..2949)
                     /locus_tag="P5293_05605"
     CDS             complement(1780..2949)
                     /locus_tag="P5293_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRR49411.1"
                     /translation="MKRILTLVMMLAVVSAYAQQQETKADVKYNQYLTNGFWDNWFVG
                     VGGGAQVYFGEHDGKIDFGDRLAPAFDISLGKWITPLFGVRGQFAGIGVKGGMIQNQG
                     KFIDKQYNGCANCYTEKFNMFNLHGDFLWNVSNTIGGYRTDRTWSFIPYAGFGWARSW
                     KEDVNPKFNEVGATIGLINKVRISDAVDFNIEIKTLLVNDRFDGYEVGQGIEELATAT
                     LGVTYNFMKRDFAKYVKPVPCDYTPYTSKIADLEKKIAETGNKAKQLENELNAEKAKG
                     PEIITNTEYLAGKMAVWFAINSSKLSDKDLINLGEYAEVIKKSGLTYKISGFADKQTG
                     NKRINKKLSEDRVNRVYDALVNKFGVNPSQLQKVAFGDEQQPFDKAPLNRVVILE"
     gene            complement(3053..3505)
                     /gene="rplI"
                     /locus_tag="P5293_05610"
     CDS             complement(3053..3505)
                     /gene="rplI"
                     /locus_tag="P5293_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRR49412.1"
                     /translation="MEIILKQDVPNLGHKDDVVTVRNGYAVNYLIPKGFAIMATPSAK
                     KVHEENMRQRAHKEAKIREDAMALATKLEGLQVKILTKVSSTGKIFGSVNNIQVADAL
                     ALQGYEIDRRNITITSEENVKEVGVYDAIVKCYRDIKANIKIEVVGEE"
     gene            complement(3518..3787)
                     /gene="rpsR"
                     /locus_tag="P5293_05615"
     CDS             complement(3518..3787)
                     /gene="rpsR"
                     /locus_tag="P5293_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRR49413.1"
                     /translation="MASQNNEIRYLNPPTVEVKKKKYCRFKKAGIKYVDYKDPEFLKR
                     FLNEQGKILPRRLTGTSQKFQKKVAQAVKRARHLALLPYVTDLLK"
     gene            complement(3801..4145)
                     /gene="rpsF"
                     /locus_tag="P5293_05620"
     CDS             complement(3801..4145)
                     /gene="rpsF"
                     /locus_tag="P5293_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRR49414.1"
                     /translation="MLKQYETVFIATPVLSEAQIKETVKKFRDFITDNQGEIVHEEDW
                     GLRKLAYPIQKKTTGFYHLIEFKADPSFITKLETQYRRDERIIRFLTFAMDKHAIAYA
                     EKKRANMAENKN"
     gene            complement(4225..5682)
                     /locus_tag="P5293_05625"
     CDS             complement(4225..5682)
                     /locus_tag="P5293_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRR49415.1"
                     /translation="MSIANLNPTRVWKHFESLTRIPRPSKKEAKAVMFLENFGKSLGL
                     ETLKDELGNVIIRKPATKGMENRKGIILQAHIDMVPQKNSDKVHDFEKDPIEPRIVDD
                     WVYATGTTLGADNGMGVAVAMAVLEAKDLVHGPIEVLITVDEETGMTGARGLKPGLLK
                     GDILINLDSEDEGELYVGCAGGLDATATFQYTTVYVPADYIGLSLAITGLKGGHSGMD
                     IVLGRGNANKALVRLLLPLVEKSGAMIASFEGGSLRNAIPREAFATLAIPASKISTAR
                     KIVSKHFSDMKAELAAQEPNLNIELNITKRPVRAIDPEIALKAVKAIYACPNGVERMS
                     DAMPGLVETSNNLAIVKSGNGKIDVKSLMRSSVDSAKEVHAIALKSVFDLAGAKVSLT
                     GGYPGWKPNMDSDILKIMKGVYKKLYGKEPEVKAIHAGLECGILGGTYPNWDMISCGP
                     TIKSPHSPDERVNIKSVEKWWNYVVEVLKEVPVKK"
     gene            complement(5719..6792)
                     /locus_tag="P5293_05630"
     CDS             complement(5719..6792)
                     /locus_tag="P5293_05630"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HRR49416.1"
                     /translation="MKFLKIYLLSIFLLLTSIAGSQTLTQGETVPLGAIVYSLPSTTI
                     HFNVTADHESFVAGPYAKFAQKYLGIQAREDNMEIYKLKSVEITPYIEADYSANYAVN
                     LGNSKNASANFLEFISQGLIMWSDSYAGKSDKMRFHNITSSQLFNPASVSNNLTSEQT
                     TLYKTVQTAKGLEKVGVQQSQVVEKSIERRAEEAANLIFKLRTKRLEIITGETDATFS
                     GDALRAAIEEINRIEDEYMSLFLGKSIFDIQEMSYDVVPSQDNQKQIYIAFRFSETQG
                     LLPASNIAGRPVVLELSLEDKVAPAPKTDISGGKGSIVYRRPAAMVAKVIDGQNILLQ
                     TRVPVYQLGNLLSFPIEVATAKK"
     gene            complement(6815..7690)
                     /locus_tag="P5293_05635"
     CDS             complement(6815..7690)
                     /locus_tag="P5293_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRR49417.1"
                     /translation="MYRIPYYLTLIFAYLFSLLPKKIHYLISDFSAWLLRDVIKYRLD
                     VIIVNIARSFPEKKYDEIKLIVDKFYHNFTDIFFEMIWSVSRSISKWRDNLETYGLDI
                     VNSAYKRNGSAMIVMGHFANWEQMLAIDMSPSGYRNEDLRIVYKKLSSQFSDFMIQWV
                     RKRHSEAALVEMNEIARYMLKHRDEKLCYIFIADQVPLPGSKFQTKFLNQPTRFFEGP
                     EQLSRKLNIPVIFLKAKRVGRGKYNAKFVSITENPSSLEEGEITNRFASLLEEAIVQS
                     PADWLWSHRRWKRDN"
     gene            7783..>8134
                     /locus_tag="P5293_05640"
     CDS             7783..>8134
                     /locus_tag="P5293_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49418.1"
                     /translation="MFKSHPKGLLAAALANMGERFGFYIMMAILTLFISAKFGLSETS
                     TGYIYSAFYASIYVLAVVGGLIADKTRNYKRTIFWGLILMSFGYLIIAIPTPTPVSSV
                     PLYLSITCVGLLVIA"
BASE COUNT         2440 a         1740 c         1264 g         2690 t
ORIGIN      
        1 acttcaaaat atatacaaat aaatctccca tagcctttac ttttttgatg atccctctac
       61 ctgacttata agcttcttaa gctcttcaag cgaaacttcc tcttcttcga taagtgagga
      121 tacaactcca agatatgagc tcttaaagta ctttttaaca acattatcca atgccccttt
      181 cttgtaatca tctcttgata caagaggata atactgatat gtattcccaa atgagtgatg
      241 gctcagaaat cctttttctt ccaatcctct tacaatagta gataaagtat tgaaatgagg
      301 ctttttatct gcataaaaat ctataagttc tcttacaaaa agagctcctt tctcccaaaa
      361 gtgagacata atctcctctt ccctggctgt tagtttttgc attattcaat cttactaaaa
      421 tctaaataca aatataacta taaaatttag ttttgcaact attttttata gttataatgt
      481 ttaacaaact aataaaacga ataatgaaac agagtgacaa aatgattgtc aactcaatca
      541 ctcaataatg acaaccctgt taagaacagg tctgttatat ggctcttctc tatcaccctt
      601 agctatcacc tcgagttgcg aaggattaac accaaacttg tttacaagag catcaaacac
      661 attctttgca cgctgttcac taagcctttg gttaatcttg ggattacctg tctccttgtc
      721 tgcataaccg gtaatcttgt aagttttgcc tgattgtttc ataacttcgg cataattacc
      781 aatattaatc atatcagtag tagaaatttt tgcactgtta attacaaacc agacagacat
      841 cttgcccata agatattctt tgacagtgac aacttcaggt gctttgtttt tttctgcatt
      901 caattcttct gcaagtctac tcgcttctgc atcagcatca gctatctttt tctcaagatc
      961 agaaatctta ttggtatatg gtgtataatc acaaggcaca ggttctacat attttgtaaa
     1021 atcacgcctg ttaaatttat aggtaaggcc aatagttgct gatgccatac cctcgccttt
     1081 aaaaccgcca actgttccgt caaatctctg gtgaacaaac aaccctctaa gttcaagatt
     1141 tgcatcaaga gcatcagaga gtcgaatctt attaatcaat cctccagaaa ctcccacttc
     1201 gttatactcg ggattgacat cctccttcca ggattttgcc caaccaactc cgacaaaagg
     1261 aacaaactgc cagaatcttg tctgtttata acccccaaca aaattggaaa gattaagaat
     1321 gtaatcagca tggagattta aaacattaaa agattcatct ccattattat cagcatacaa
     1381 agcattaggt ccgtatcctt tggcatcgat acctgcatat tggaaacgga atcctgaaca
     1441 tggggtaatc catttgccaa cagagaaatc caaagcagta gatattcgat caccaaatga
     1501 aagctcactg tcattctctc caaaatagac attaccacca actcctgcag atatgaacca
     1561 attatcccag aaaccgtttg tcaaaaacgg ccctcttttg gactgttcct gggctgaaat
     1621 aatcccaaaa aacatcaaaa gaactaaggt taaaaaactt ttcttcatag cattatcgag
     1681 ttaatttcgt gaaagataat aaaaaatatt tcaatcagaa aatattttta caaaaaaaaa
     1741 cctatcctta cggacgggct taatttgtaa tgttttaaat tattctaata ttacaacacg
     1801 atttaatgga gctttgtcaa acggctgttg ttcatcgcca aatgcaactt tttgaagctg
     1861 agaaggatta acaccaaact tatttacaag agcatcataa accctattta ccctatcttc
     1921 gctaagtttc ttgttaattc tcttatttcc agtctgttta tcagcaaaac cggagatttt
     1981 gtaagtcaag ccagatttct taattacttc tgcatattcg ccaagattaa taagatcttt
     2041 atcgctcaat ttactactgt taatagcaaa ccaaacagcc atcttacctg caagatactc
     2101 agtattggta ataatttcag gcccttttgc cttttctgca ttcaattcgt tctccaattg
     2161 ttttgccttg ttgccagttt ctgcaatttt tttctcaaga tcagcaatct tgcttgtata
     2221 aggagtatag tcgcatggaa caggtttaac atatttagca aaatcacgtt tcataaaatt
     2281 gtaagtgaca cctaaggttg cagttgcaag ttcttctatt ccctgtccaa cctcatatcc
     2341 atcaaatctg tcattaacca atagtgtttt aatctcaatg ttaaagtcca cagcatcaga
     2401 aatacgaact ttgtttatta gacctatagt agcaccaact tcattaaatt tgggattgac
     2461 atcttctttc catgatcttg cccagccaaa tcctgcatat gggatgaatg accatgttcg
     2521 gtctgttcta tatcctccaa ttgtattaga cacattccag agaaaatcac cgtgaaggtt
     2581 aaacatgttg aatttttcgg tatagcaatt cgcacatccg ttgtattgtt tatcaataaa
     2641 tttaccttga ttctgaatca tgccaccttt tacgcctatg ccagcaaatt gtccacgaac
     2701 gccaaaaagt ggtgttatcc acttaccaag tgatatatca aatgcgggag ctaatcggtc
     2761 gccaaaatct atcttcccgt catgttctcc aaagtagacc tgggctcctc cccctactcc
     2821 aacaaaccag ttatcccaaa aaccatttgt aaggtattgg ttatacttta cgtcggcctt
     2881 tgtttcttgc tgttgagcat atgctgacac aacagcaagc atcatgacta aagttagaat
     2941 cctcttcata ataattccgt ttagtttaaa attaaaaaat ctaataccaa caatttatct
     3001 tatacaaaga taatacgaaa aataaaacat ccaaaaaaga gaagagagga ttttactcct
     3061 ctccaacaac ctctatcttg atattagcct taatgtccct gtaacactta acaatcgcat
     3121 cataaacacc tacttcctta acattttcct cacttgttat ggtaatgttt ctgcgatcta
     3181 tctcgtagcc ctgaagcgca agggcatctg ctacctgaat attgtttaca gaaccaaaaa
     3241 tcttgcctgt agaacttacc ttagttaaaa ttttaacctg caatccttca agttttgtcg
     3301 ccaatgccat tgcatcctca cgaatcttag cctctttatg agccctttgt ctcatatttt
     3361 cttcatgaac ctttttggca gagggagtag ccataattgc gaaacctttt ggtatcaggt
     3421 aatttacagc atatccattg cggactgtta ctacatcgtc cttatgaccc agattgggaa
     3481 catcctgttt aagtataatt tccataaaat ctccctccta tttcaataag tcagttacat
     3541 aaggtaatag cgcaaggtgt ctggcacgtt taactgcctg ggcaaccttc ttctgaaatt
     3601 tctgtgatgt accggtaaga cggcgtggca aaattttacc ttgctcatta agaaatcttt
     3661 taagaaattc aggatcctta tagtccacat acttaatacc ggccttttta aaacgacagt
     3721 attttttctt ctttacctca acagttggtg ggttcagata acgtatttca ttattttgac
     3781 ttgccataat tttactcttc ttagttttta ttttctgcca tatttgcacg tttcttctct
     3841 gcataagcta ttgcatgctt atccatagca aaagtaagaa aacgaataat gcgctcatca
     3901 cgacgatatt gagtttcaag tttagtgata aatgaagggt ctgccttaaa ctcaattaaa
     3961 tgataaaaac ctgtggtttt cttttggata gggtaagcca actttctgag tccccagtcc
     4021 tcttcgtgaa cgatttcacc ctgattgtcg gtgataaagt cacgaaattt ttttaccgtt
     4081 tcctttatct gagcttcaga taaaacggga gtagcaatga aaacggtttc gtactgtttt
     4141 aacatactct ttttgtgtgt ttattaaatt ataaaattat ttaaaaaggg ctgcaaagtt
     4201 ataaaataat tatattcaaa caaattattt cttaacaggg acttccttaa gaacttcaac
     4261 tacatagttc caccactttt caacactttt aatattcact ctttcatccg gagaatgagg
     4321 agatttgatg gttggaccac acgaaatcat gtcccaatta ggatatgttc ctccaagaat
     4381 gccacattcc aaacctgcat gaatggcttt tacctcaggc tctttgccgt aaagcttttt
     4441 ataaacgccc ttcatgattt tcaagatatc cgaatccata ttcggcttcc agccaggata
     4501 accacctgtc agactgactt tagctcctgc aagatcaaaa actgacttaa gggcaattgc
     4561 atgtacttcc ttagcagaat caacggaact tctcataaga gacttaacat ctatcttccc
     4621 attcccgctt ttaacaatcg ctaggttgtt ggaagtttct acaagccccg gcatcgcatc
     4681 actcattctc tcaaccccgt ttggacatgc atagattgcc ttaactgcct tgagagcaat
     4741 ttctgggtca attgctctaa caggtctttt agtaatatta agttcaatat tgagattcgg
     4801 ttcctgagca gcaagttctg ctttcatgtc gctaaaatgt ttgcttacta tctttctggc
     4861 agttgatatc tttgaggcag gaatcgccaa agttgcaaaa gcctctcttg gaattgcatt
     4921 tctcaacgaa ccaccttcaa aggatgcaat catggcacct gatttttcaa cgagaggcag
     4981 aagtaatctc actaaagcct tgttggcatt tccacgacca agaactatat ccatacctga
     5041 atggcctcct ttaaggccgg ttatagcaag ggaaagtcca atataatctg caggaacgta
     5101 aactgttgta tattggaatg ttgccgttgc atccagacca cctgcacatc cgacataaag
     5161 ttctccttcg tcttctgaat cgagatttat caatatgtca cctttcaaaa gacccggttt
     5221 taaacctctt gctcctgtca tccctgtctc ttcatcaaca gtaatcaata cttcaatagg
     5281 cccgtgaacg agatctttcg cttcaagaac agccattgcc acagctactc ccattccatt
     5341 atcagcacca agggtggtac ctgttgcata tacccagtca tccacaatcc tgggttcaat
     5401 tggatctttc tcaaaatcat gaaccttgtc actatttttc tgaggtacca tatctatatg
     5461 tgcctgtaga ataatccctt tacgattttc catacctttg gtagcaggtt ttcgtataat
     5521 aacgtttcct agttcatcct ttaatgtttc gagacccaaa cttttcccga aattttccag
     5581 aaacattaca gccttggcct ctttctttga aggacggggt atccgggtaa gagattcaaa
     5641 atgtttccat acccttgttg gatttaaatt agcaatactc atttctataa atattaaaat
     5701 ttgttaagat taaacaattt acttttttgc tgttgccacc tctatgggaa atgatagcaa
     5761 gttccccaac tgatatacag gaactcttgt ctgtaaaagg atattctggc catcaataac
     5821 tttagccacc atagctgccg gtcttctgta cactatactt cccttacctc ctgaaatgtc
     5881 tgtcttaggc gcaggtgcaa ctttatcttc caaagaaagt tccaaaacaa caggccttcc
     5941 ggcaatattt gatgctggca ataaaccttg agtttcagaa aatctgaagg ctatatatat
     6001 ctgtttctgg ttgtcctgtg aaggaacaac gtcataactc atttcttgaa tatcaaatat
     6061 gcttttacct aaaaaaagcg acatgtattc atcctctatt ctgttaattt cctctattgc
     6121 agctctcaac gcatcaccac taaaagtagc atcagtttct ccggtaataa tctcaagcct
     6181 ctttgttctc agtttaaata taagattagc agcttcttct gctcttcttt cgatgctctt
     6241 ctccacaacc tgactctgct gaacgcccac cttttcgagg ccttttgcag tctgaacagt
     6301 tttataaagc gtggtttgct cgcttgtaag attattagaa acacttgcag gattaaacag
     6361 ttggctagaa gtaatattgt ggaacctcat cttgtcactt ttcccggcat atgaatctga
     6421 ccacataatt aacccctgac ttataaattc aagaaaattg gcagaagcat tcttagaatt
     6481 acccaggttg actgcataat tagcagagta atccgcttca atgtaagggg taatctccac
     6541 tgatttcaat ttgtaaattt ccatattgtc ctctctggcc tgtatcccaa gatacttttg
     6601 ggcaaatttg gcataaggac cggccacaaa ggactcatgg tctgccgtta cattaaaatg
     6661 tattgtagta cttggaagag aatatactat tgctccaagt ggtactgtct ctccctgcgt
     6721 taaagtctga gatcctgcaa ttgaggttaa tagcagaaat atggacagta ggtatatttt
     6781 caaaaatttc ataatattga atatttataa attattaatt gtctcttttc caccttctgt
     6841 gtgaccataa ccaatctgca gggctctgaa caattgcttc ttcaagcagt gatgcaaatc
     6901 tgtttgttat ttcaccctcc tcaagagatg aaggattttc tgttatagat acaaatttag
     6961 cattgtattt accacgtcca actctttttg ctttaagaaa tataacagga atattcaact
     7021 ttcttgaaag ttgttcgggt ccttcaaaaa aacgagttgg ttgatttaaa aattttgtct
     7081 gaaattttga accgggaagc ggtacttgat ctgcaataaa tatataacac agtttctcat
     7141 ctctgtgttt aagcatatat ctggctattt cgttcatctc cacaagcgct gcttctgaat
     7201 ggcgtttcct tacccattgt atcataaaat ccgaaaattg gctactcaat ttcttatata
     7261 caatcctcag gtcttcattc ctgtatccag aaggcgacat atctatagca agcatctgtt
     7321 cccaatttgc aaaatgtccc atgacaatca ttgcagagcc atttctttta taagctgaat
     7381 taactatatc caaaccgtat gtctcaagat tatctctcca ttttgaaata gatcttgaaa
     7441 cagaccatat catttcaaaa aaaatgtcgg taaaattgtg ataaaattta tcaacaatta
     7501 acttaatctc atcgtacttt ttctcaggaa aagatcttgc tatgttgaca ataatcacat
     7561 ccaacctgta tttaataacg tctctcaaca accaagccga aaaatctgat attaaataat
     7621 ggattttttt gggcaacaat gaaaaaagat aggcaaatat aagagtgagg taataaggaa
     7681 tcctatacat tagttgattt tacattttta acaaagttat ccaaatttta gtatttttgt
     7741 agagttatga ggaattttta actaatctaa aattaatacc atatgtttaa atcacatccg
     7801 aaaggactac tggcagcagc tctcgcaaat atgggagagc gcttcggatt ctacattatg
     7861 atggcaatat tgacattgtt catctctgca aaattcggtc tgtctgagac ctctacaggc
     7921 tatatttact ccgctttcta cgcatcaatc tatgttcttg ctgtagttgg aggtttgatt
     7981 gccgacaaga ctcgtaatta taagcgtact attttttggg gacttatttt gatgtctttt
     8041 ggctatttga ttattgctat tcctactcct actcctgtat caagtgttcc gctctatctt
     8101 tcaataactt gcgtaggttt gttggtaatt gcct
//
LOCUS       DAOTQL010000079         8004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7690_length_8004_cov_5.126054, whole genome shotgun sequence.
ACCESSION   DAOTQL010000079 DAOTQL010000000
VERSION     DAOTQL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7690_length_8004_cov_5.126054"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1111
                     /locus_tag="P5293_05645"
     CDS             164..1111
                     /locus_tag="P5293_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRR49419.1"
                     /translation="MLEIFYKNRGQVLTSNELSALENLGYDDVLWIDLSSPTGEEKRA
                     IEEFLNTNLQSRAQAEEIESSSRYSEDENTIFANTNFLIPGPESYSMEAVSFILCEGI
                     IVTIRHVPLRSFTDIQRKIIANYKTMPTGYHILVGILENRIDLDADMIELMSKEIAQY
                     SKKVSLGGAMGEDLLLDINQLQENTMLVRENIVDKQRMISGILKSDKFPRDLFPKLDI
                     LLRDINSLINHTNFSFERLEYLQNTVLGLINLEQNRIMKIFTFVSLLFLPPTLIASIF
                     GMNVELPNFGGFDFIAIMLLMVVAVFIVSIIFFRKKMLN"
     gene            complement(1104..2411)
                     /locus_tag="P5293_05650"
     CDS             complement(1104..2411)
                     /locus_tag="P5293_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49420.1"
                     /translation="MKNTNGFRSFPRNFWTAVTMEFLERGAYYSVMSILSVYLILSPE
                     TGGLGFTKEQAGAVLGIIPPLLYFLPIVAGAIAERYGYRRVLFFAFVCIVAGYLFTGL
                     SHTYALVLLSLVVLALGAGFFKPVISGTIARSTNDKNSSLGFGIYYWSINLGAFLFPL
                     ILVPILKSISYSYVFYMASILGLILILLNLFIYREPEKAVSDKSLSQVLKEMVLVIKD
                     WKFLLMILLYSGFWILYFQMYGTVLWYVKDYMDMTPVNHVVNSFLSIFVENPSWKFDV
                     EHVTVINAGVIILLQLVVSNLVKNWPALPTMIMGIGFGTIGMAILSLSTSPWVFICGI
                     MTFTLGEMTTHPKFISYIGMIAPSDKKALYLGYSFLYGVIGSSIGGFLGSRLYVKYVD
                     NMGNPSALWLIFSCIGIFSIISLLLYNKFLTPKKMLSDSVKVN"
     gene            2548..4473
                     /gene="rpsA"
                     /locus_tag="P5293_05655"
     CDS             2548..4473
                     /gene="rpsA"
                     /locus_tag="P5293_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRR49421.1"
                     /translation="MVKDLKKEELEQEEPVLPVSREEKLEAFIDDDSAAVIAEEAANK
                     RKSNASVSGDNFDWDAFEKETMYDADKSTIEDSYSQTLSKVIENEVVEGTVVNVNKRE
                     VIVNIGYKSEGVISINEFRYNPDLAVGDKVEVYVENAEDKKGQLILSHKKARSLRSWD
                     RVNEAFEKDEIIKGYIKCRTKGGMIVDIFGIEAFLPGSQIDVKPIRDYDVFVNKTMEF
                     KIVKINHEFRNVVVSHKALIEAELEAQKADIIGKLEKGQILEGVVKNITSYGVFVDLG
                     GVDGLIHITDLSWGRINHPEEVVQLDQKINVVILDFDDTKKRIALGLKQLSSHPWDSL
                     DPNLKVGDKVKGKVVVIADYGAFIEIQPGVEGLIHVSEMSWSLHLRSAQDFLKVGDEV
                     EAVILTLDRDERKMSLGIKQLKPDPWATIADKYPVGKRCTATVRNFTNFGVFVELEEG
                     VDGLVHISDLSWTKKIKHPSEFTSVGDKLDVVVLEVDQENRRLSLGHKQLEENPWDVF
                     ETVFTVGSIHEGTITSFVDKGATVALPYGVEGYVSMKNLVKEDGTTGKLEEKLDFKVL
                     EFNKSNKRVLLSHSKIFEDAKKEEQKEKHESEADSTQKAVKKLKANLEKTTLGDISEL
                     AALKSEMESKDQENKQQ"
     gene            4474..5394
                     /locus_tag="P5293_05660"
     CDS             4474..5394
                     /locus_tag="P5293_05660"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRR49422.1"
                     /translation="MKFSFSSLGCASALPTVNRFPSAHVLNVHERLFLIDCGEGCQMQ
                     LRRFGFSILKIQNIFISHLHGDHVFGIYGLLSTMSMLGRTAPIFIYAPSEFSEILSEF
                     MRHFGDMFKYEINHIVLNGKNIEIIFQGRNFEVLSFPLNHRIKCYGFLFREKKPLRNV
                     VKSRIEEKSLTLSEIAMLKSGRDVIREKEVLLNVDYTYIPYEPRSFAYCSDTAPFEEL
                     VSYISGVDLLYHEATFSSEMAEMAVATMHSTASDAALAAKKAGAGKLVIGHYSSRYKD
                     LGKLLDEAKEIFPETYLAEEGIEFDIPMKI"
     gene            5407..6993
                     /locus_tag="P5293_05665"
     CDS             5407..6993
                     /locus_tag="P5293_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR49423.1"
                     /translation="MKRIKYVLPLLSLTLFSLATSAQVINDVNKQVTKYGQAIFYINS
                     YYLDTVNVEMLTEEAVKSVMSKLDPHSTYISAKDVKSMNEPLEGNFEGVGIEFAIIGD
                     TLTVTAPIAGGPCERVGIRTGDRIIMVDGELITGIGLTTEKVHKYLRGPKGTKVTLGV
                     VRKDVPERLDFVVERDKIPINSLDAAYEVANGVYYLKLGRFAQNSSEEILKALIDLKA
                     NKINGIILDLRSNSGGFLGTALEIANFFLDGGQTIVYTEGLRSPKRVEVATGTGFYKK
                     GPLVVMIDENSASASEIVAGAIQDWDRGTIVGRRSFGKGLVQQMLPLNDGSQLRLTVA
                     RYHTPSGRVIQSPYKAGEADNYYKAFLERYKSGELFNRDSISFPDSLKYKTLVKGRTV
                     YGGGGIMPDIFVPADTSKYSDYYSNLIRKGILNDFMSGLGDKNRVEWSAKYKTFAQFE
                     KEFRIDDQMLKDLVSFAEKKSLPLDEKGFETSKDEIVVIMKALAARTVFGSSAYYRVI
                     NAESDETFKKALELINSSGL"
     gene            complement(6974..>8004)
                     /gene="mltG"
                     /locus_tag="P5293_05670"
     CDS             complement(6974..>8004)
                     /gene="mltG"
                     /locus_tag="P5293_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRR49424.1"
                     /translation="INVNFKKIHKIILVVLLTVSFILASGIYFTFFRPNVKGEMTYLY
                     VPHNSEYKQVIDSLEKSGCIKSIMSFKIAAKIEHLDKKISPGRYKIEKGMNNLYIIRA
                     IANGWQTPVRLTISGNIRNLEKLASIISKKIESDSSEIISVLRDTCLIDSLGFDPTTF
                     PTMFLPDTYEVFWTVSPKELLFKLKREYDKFWNEERVAKASKIGLTPVEVSILASIVS
                     EESNIKDEQPVIAGVYLNRLKRGMKLQADPTIKFILNDPSIKRILNKHLTIDSPYNTY
                     IHKGLPPGPIVIPQKSTIDAVLNYKQHDYFYFCAAPTLNGSHNFARNITEHNKNARAY
                     QRAISRKN"
BASE COUNT         2511 a         1275 c         1719 g         2499 t
ORIGIN      
        1 tgctgacaat gtctcattta atcttagtgc agataaaaga aagggtctgg cggaattttg
       61 gtcacttgca cttgaggaac ttaagtcaaa agtccgatgg tttggctaaa ttgataccct
      121 aaaccggtaa atatttagta ataaaccatt aaatgatttt gacatgcttg aaatatttta
      181 caagaataga ggacaggtac taacctcaaa tgaactaagt gctcttgaga atcttggcta
      241 tgacgatgtg ttgtggattg atttgtcttc tcctacaggt gaggagaaga gagctataga
      301 ggagtttctg aataccaatc ttcaaagccg tgctcaagcg gaggaaattg aaagttcttc
      361 tcgctattcg gaagatgaaa atacaatttt tgccaatacc aattttttga tacctggtcc
      421 agaaagttac agcatggagg cagtttcgtt tattctttgc gaaggaataa tagtcactat
      481 ccgtcatgtt ccgctgagga gctttactga tatacagaga aaaattattg ctaattataa
      541 aaccatgcct accggttatc atatattggt aggtattctc gaaaacagga ttgacctgga
      601 tgctgacatg attgaattga tgtcaaagga aattgctcaa tacagtaaga aagtaagtct
      661 tggtggggca atgggtgaag atcttcttct ggatattaat cagttgcagg agaacactat
      721 gttggtcaga gagaacatcg ttgataaaca aaggatgata tcgggtattc ttaagagcga
      781 taagtttcca agggaccttt ttcccaagct tgatatactt ttgagagata taaattctct
      841 tattaatcat acaaatttta gtttcgagag gctagagtac ttacaaaaca ctgttttagg
      901 tcttattaat ctggaacaga accgaataat gaagatattt acttttgttt ctcttctctt
      961 tttgccacct acacttattg caagtatttt cggaatgaat gtggaactgc caaattttgg
     1021 aggatttgat tttattgcca taatgctgtt aatggtagtg gcggtattta ttgtctcaat
     1081 tatctttttc agaaaaaaga tgctcaattg acctttacgc tatctgaaag catcttttta
     1141 ggtgtgagaa atttgttgta cagaagtaga gaaatgatac tgaaaatgcc tatgcacgag
     1201 aatatcagcc aaagtgcaga agggtttccc atattatcta catatttgac atagagcctt
     1261 gaaccaagaa aacctcctat actgcttcct ataactccat atagaaacga ataaccgaga
     1321 tagagggcct ttttatcgga aggggctatc atccctatat atgatatgaa ttttggatga
     1381 gttgtcatct ctcccaatgt gaatgtcatg atgccacata taaaaaccca tggacttgta
     1441 gatagagaca gaattgccat tccaatcgtg ccaaacccaa ttcccatgat cattgtagga
     1501 agagccggcc aatttttcac cagattggag acaacaagtt gaagaagaat aataactcca
     1561 gcattaatta ctgtcacatg ctctacatca aatttccatg agggattttc tacgaaaatt
     1621 gacagaaatg aatttacaac atgatttaca ggtgtcatat ccatgtagtc cttaacatac
     1681 cataatacag ttccatacat ttgaaaatag agaatccaga atccggagta gagtaatatc
     1741 ataagcagga acttccaatc cttaattaca agtaccatct ctttaagtac ttggctcaga
     1801 cttttatctg agacggcttt ttcgggttct ctatagataa agaggtttag aagaattaat
     1861 atcaatccca aaatagaagc catgtaaaat acatagctgt atgatatgct ctttagtatt
     1921 ggcactaaaa taagtggaaa aagaaatgct cccagattaa ttgaccaata ataaatgcca
     1981 aatccaaggc ttgaattttt gtcatttgta ctccttgcga ttgtgcctga aataaccggt
     2041 ttgaagaatc ctgcccccag agcaagaact acaagtgaaa gtaaaacaag cgcgtaagta
     2101 tgagaaaggc cggtgaatag atatccggca actatgcata caaatgcgaa gaaaagtacc
     2161 cgtctgtatc catatctttc tgctattgct cctgcaacta tgggcagaaa atagagcaac
     2221 ggggggatta ttccaagaac tgcacctgcc tgttcttttg tgaatccgag tccaccagtc
     2281 tcaggagaga gtatcaggta aacagagagt atggacatca ctgaataata ggcaccacgt
     2341 tcaagaaatt ccattgttac agctgtccag aaattccgtg ggaaggagcg aaatccattt
     2401 gtgtttttca taacttttct ttttccaaag ataattcaaa atggctgttt gcagttattt
     2461 tgtataagcg aaaattatat ttaattttgc caaattatag gatatactat aaataatgtc
     2521 taactacttg aattttaaaa attaaaaatg gtaaaagatc ttaaaaaaga agaactcgaa
     2581 caggaagaac ctgtgctccc tgtgagcagg gaagagaaat tggaagcttt catcgatgat
     2641 gattctgctg ctgttattgc agaagaagca gccaataagc gtaaaagtaa tgcctctgta
     2701 tctggcgata attttgattg ggatgcattt gaaaaagaga ctatgtacga tgctgacaaa
     2761 tctacaatcg aggacagtta cagtcaaact ctttctaaag taattgaaaa tgaggttgtt
     2821 gaggggacgg ttgttaatgt taacaaaaga gaggtgatag ttaatattgg ttacaaaagt
     2881 gagggtgtta taagtatcaa tgaatttcgt tacaatcctg acctggctgt aggggataag
     2941 gttgaggtat atgttgaaaa tgcagaggac aagaaaggac agctcattct ttcgcataag
     3001 aaagctcgct cacttcgttc ttgggacaga gttaacgagg cttttgaaaa ggacgagatt
     3061 attaaaggat atattaaatg cagaaccaaa ggtggcatga ttgtagacat atttggaata
     3121 gaagcatttc ttcctggttc gcagatagat gtcaaaccta taagggatta tgatgttttt
     3181 gtcaacaaga ctatggagtt taagatagtc aagataaatc acgaattcag aaacgtggtt
     3241 gtttctcaca aggctctcat agaggccgag cttgaggcac aaaaggccga tattataggt
     3301 aaacttgaga aaggtcagat tttggagggt gttgtcaaga atataacatc atatggtgta
     3361 tttgtcgatc ttggcggcgt tgacggtcta attcacatca cagatctttc atggggcaga
     3421 ataaatcatc ctgaggaagt tgttcagcta gatcaaaaaa taaatgttgt tattcttgat
     3481 tttgacgaca caaagaagcg tattgctctt ggacttaagc aattgagttc tcatccatgg
     3541 gactctcttg atcctaacct taaggttgga gacaaggtaa agggtaaggt tgtggttatt
     3601 gctgattatg gtgcatttat tgagatacag cccggtgtag aaggtttgat ccatgtctct
     3661 gagatgtcgt ggtctcttca ccttcgcagt gctcaggatt ttcttaaagt tggagatgaa
     3721 gttgaggcgg ttattcttac ccttgacagg gatgaaagaa agatgtctct tggcatcaag
     3781 caacttaaac cagatccttg ggctactata gcagataaat atcctgttgg taaaagatgc
     3841 actgcaacag tccgtaattt tacaaatttt ggtgtttttg ttgagctcga agagggagtt
     3901 gacggtcttg ttcatataag cgatctttca tggactaaaa agatcaaaca tccttcagaa
     3961 tttacctctg tcggagataa acttgacgtt gtggtgcttg aggtagatca ggaaaaccgt
     4021 cgtctcagcc tgggtcacaa gcagcttgag gagaatcctt gggatgtttt tgaaacagta
     4081 tttactgttg ggtctataca tgaaggtact attacctctt ttgttgacaa aggtgcaaca
     4141 gttgctcttc catatggagt tgaaggatat gtcagcatga agaaccttgt aaaagaagac
     4201 ggtactacag ggaaactcga agagaagctt gattttaagg ttcttgaatt taataagagt
     4261 aataagagag tcttactttc acactcaaag atatttgaag atgccaagaa agaagaacag
     4321 aaagagaagc atgagagtga ggcagactca acacaaaaag ctgtgaagaa attgaaagct
     4381 aatcttgaaa agacaacttt gggagacata agtgaacttg cagctcttaa aagtgaaatg
     4441 gaaagtaaag accaggaaaa caagcagcag tagatgaaat tttctttttc atctttgggt
     4501 tgtgcttcgg ctcttcccac tgtgaacaga tttccaagcg ctcatgtcct taatgtgcat
     4561 gagcgcttgt tccttatcga ttgtggtgaa ggatgccaga tgcaattgag aagatttgga
     4621 ttttcaattc tgaaaatcca aaatattttt atttctcacc ttcatggtga tcacgttttt
     4681 ggaatttatg gtctgctttc aaccatgtca atgcttggaa gaactgctcc aatatttatt
     4741 tatgcacctt ctgaattttc agagattctt tctgaattta tgagacattt tggtgacatg
     4801 ttcaagtatg aaataaatca tatagttctt aatggaaaaa acattgaaat tatttttcag
     4861 gggagaaatt ttgaagtact gtcatttcct ctaaatcaca ggataaaatg ttatggtttt
     4921 ttattcagag aaaaaaagcc tttgcgcaat gttgtcaaaa gtcgtattga agagaaatcg
     4981 ctgactctta gcgagattgc catgttgaaa agtgggaggg atgtgataag agaaaaagaa
     5041 gttttgctta atgttgatta tacttatatc ccatatgaac ctcgttcttt tgcttattgc
     5101 agtgatactg ctccttttga agaacttgtt tcgtatattt ctggagttga tttgctttat
     5161 catgaagcaa ccttttcttc tgaaatggcc gaaatggctg tagccaccat gcactctacg
     5221 gcttctgatg cagcacttgc tgctaaaaaa gcaggtgcag gcaagcttgt gataggtcat
     5281 tactcttctc gttacaaaga tttgggaaaa ttgcttgacg aagcaaagga aatatttcct
     5341 gaaacatatc ttgcagaaga gggcatagag tttgatatac caatgaaaat atagaaaatt
     5401 aattttatga aaagaattaa atatgtttta cctcttttgt ctctgacatt gttttcactt
     5461 gcgacttcag cacaagttat taacgatgtt aacaaacagg tcacaaaata tgggcaggct
     5521 attttttaca tcaatagtta ttatttggat acagtaaatg ttgaaatgtt gactgaggag
     5581 gctgttaagt cggtaatgtc aaaacttgat ccacattcta cttacatatc agccaaggat
     5641 gtaaaatcca tgaatgaacc gttggaaggc aattttgaag gtgtgggtat cgaatttgcc
     5701 attattggag acacactaac tgtaacagct cctattgcag ggggtccgtg tgaaagagta
     5761 ggaataagaa caggtgacag gattataatg gttgatggag agttaattac ggggataggt
     5821 cttacaaccg aaaaagtaca taaatatctt agaggcccaa aaggaactaa agttacactt
     5881 ggtgtagtta gaaaagatgt gcctgaaaga cttgattttg tggtagaaag agataaaatt
     5941 cctatcaata gtcttgatgc agcctatgaa gtcgcaaatg gagtttatta tctgaagctt
     6001 ggcaggtttg cccaaaattc ttctgaagag atacttaaag ctcttattga tttaaaggct
     6061 aataagataa atggtattat tcttgatctg agaagtaatt caggaggttt tttgggtaca
     6121 gcgctcgaaa ttgccaattt ttttcttgat ggaggccaga caattgtata tacagaagga
     6181 ctaagatctc caaagagggt agaggtagct actggcacag gtttttataa aaaaggtcct
     6241 ttggtagtaa tgattgatga aaattctgct tctgcaagtg aaattgttgc aggcgccatc
     6301 caggattggg acagagggac aatagtgggc agaagatctt ttggcaaagg gcttgtgcag
     6361 cagatgcttc ctttgaatga tggctctcag ctgaggctta ctgtggctag gtatcataca
     6421 ccttccggaa gagtaattca aagtccttat aaggccggtg aggctgacaa ttattataag
     6481 gcgtttcttg agagatataa gagtggagag ctatttaaca gagatagtat aagttttcct
     6541 gattctctta aatataagac acttgtcaag ggaagaacag tctatggtgg cggcggtatt
     6601 atgccggaca tatttgttcc tgctgatact tcaaaatatt ccgattatta ttccaattta
     6661 atcagaaagg gtattctgaa tgatttcatg agtggtcttg gagacaagaa ccgggttgaa
     6721 tggtcagcaa aatacaagac atttgcgcag tttgagaaag aattcagaat agatgatcaa
     6781 atgttgaaag acttggtctc ttttgctgaa aagaaatcat taccactcga tgagaaaggt
     6841 tttgaaacat caaaagatga aattgtggtt attatgaaag cactcgctgc aagaacggta
     6901 tttggaagca gtgcatatta cagagtgatt aatgccgaaa gtgacgaaac ctttaagaaa
     6961 gcgcttgagt tgattaattc ttccggctta tagcacgctg gtaggctctt gcatttttat
     7021 tgtgctctgt aatgtttctg gcaaagttgt gtgaaccgtt tagtgtcggt gcggcacaaa
     7081 aataaaaata atcgtgttgt ttataattga gaactgcatc tattgtgctt ttttgaggaa
     7141 taacaattgg tcctggaggt aaacctttgt gaatataggt gttatatggt gagtcaattg
     7201 tcagatgttt gttaagtatt ctttttatag atgggtcgtt caggataaat tttatggtag
     7261 ggtcagcctg aagcttcatg cctcttttaa gtctgttaag atatacacct gcgattacag
     7321 gttgttcatc ctttatattg ctctcttctg atacgattga tgcgagaatg cttacttcta
     7381 ccggtgtcaa tccaattttt gaagcttttg caactctctc ttcgttccaa aatttatcat
     7441 attctctttt tagcttaaaa agtagttctt ttgggcttac tgtccaaaat acttcatagg
     7501 tgtcaggtaa aaacattgtg ggaaatgtgg tcggatcaaa gccaagggag tcaataaggc
     7561 aggtatccct gagaacagat ataatttctg aagagtcgct ctcgattttt tttgatataa
     7621 tagatgctaa tttttccaga ttgcgaatat tacctgaaat tgtcagcctt acaggagttt
     7681 gccacccatt tgctattgct ctgataatgt ataggttgtt cattcctttt tctatcttgt
     7741 atctgccggg cgatattttt ttgtcaagat gttcaatttt ggcggctatt ttgaaactca
     7801 ttattgattt tatgcatcca cttttctcaa gactgtctat tacttgcttg tattcggaat
     7861 tgtgcggtac gtataaatat gtcatttcgc ctttgacgtt aggtctgaaa aatgtaaaat
     7921 aaattcctga agccaaaata aagctgacag tgagcaggac taccaatatt attttatgga
     7981 tttttttgaa gttcacgttt atct
//
LOCUS       DAOTQL010000080         7865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7838_length_7865_cov_7.050320, whole genome shotgun sequence.
ACCESSION   DAOTQL010000080 DAOTQL010000000
VERSION     DAOTQL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7838_length_7865_cov_7.050320"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..552
                     /locus_tag="P5293_05675"
     CDS             403..552
                     /locus_tag="P5293_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49425.1"
                     /translation="MLTDNNFLLWTNKPLDEVWDGIDTSVKVDDERGLGPMKLKGGRN
                     KAVVS"
     gene            1419..1592
                     /locus_tag="P5293_05680"
     CDS             1419..1592
                     /locus_tag="P5293_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49426.1"
                     /translation="MDKMDLLRKEIVEFRNILEKINEEYMYGSKDGWDPADYYSDVMY
                     RASIFFARISELV"
     gene            1592..1870
                     /locus_tag="P5293_05685"
     CDS             1592..1870
                     /locus_tag="P5293_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49427.1"
                     /translation="MMNELIQRIQRTRDIIYRNGDLPCMAIVHNLQNYSTILKEMGLN
                     FEAAAVLSAGSLLYNFPVYKFDQIYPDSIRYGAVILLDRVIDNLTERM"
     gene            complement(2041..2385)
                     /locus_tag="P5293_05690"
     CDS             complement(2041..2385)
                     /locus_tag="P5293_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49428.1"
                     /translation="MDRRGHYTVENTEKTGIFGTVDFVKNHYINPSTLNHEGDPSGPV
                     PSGSTFFPVTVLQHSLCIHFQTVAFPGGLVKPFRYLYILVVAILMSLSLIVITVTLSE
                     RKKNLDTGLPLT"
     gene            2438..2623
                     /locus_tag="P5293_05695"
     CDS             2438..2623
                     /locus_tag="P5293_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49429.1"
                     /translation="MKKTYTKNEIIEILLFLAEKADKDALRFENNGNMECRNISEGLH
                     HAYLTTASWLKKINIKE"
     gene            2840..2986
                     /locus_tag="P5293_05700"
     CDS             2840..2986
                     /locus_tag="P5293_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49430.1"
                     /translation="MVDENGDTNLVVDISEVHRTAFEWGFHDDDMPDIIKSVEERVFI
                     NYKE"
     gene            3222..3434
                     /locus_tag="P5293_05705"
     CDS             3222..3434
                     /locus_tag="P5293_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49431.1"
                     /translation="MSNKSPEMEKFLNDLSKMLYGRERHSGACVACGSHKVSAEDFRD
                     ALSYTEYGISNLCQECQDKIYKICQE"
     gene            3422..3652
                     /locus_tag="P5293_05710"
     CDS             3422..3652
                     /locus_tag="P5293_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49432.1"
                     /translation="MPRVKTKLNKYQVFTQTFPTPKATKAYFKLHYAKGAKIVGPLTW
                     KVKNGNYVRLYLFGIEELKSIKHLQQPKTKKY"
     gene            3710..3934
                     /locus_tag="P5293_05715"
     CDS             3710..3934
                     /locus_tag="P5293_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49433.1"
                     /translation="MKKAKMTGKEIQKQVSEARKPSTVEAKGQFKFYKDTKRYHAYNI
                     TGSHGITGVVYTPRGEDVPDIITLKFEKKS"
     gene            4271..4492
                     /locus_tag="P5293_05720"
     CDS             4271..4492
                     /locus_tag="P5293_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49434.1"
                     /translation="MKYTEKQLADMFSALADKYSALAQEALKNNDLEDYNWYGGQSSA
                     FQRTADFFCKLDNMKEKRCVNEKKTLEEN"
     gene            4640..4834
                     /locus_tag="P5293_05725"
     CDS             4640..4834
                     /locus_tag="P5293_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49435.1"
                     /translation="MRRFLIVCAEKIRELESAGLVHRAADYRDLQAEAHWKLYGYTTF
                     GAAKDRERILDDDYLKRIGI"
     gene            5521..5922
                     /locus_tag="P5293_05730"
     CDS             5521..5922
                     /locus_tag="P5293_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49436.1"
                     /translation="MDILLKEATSELCPNCDFLFHEAEVMGCCGFCGDMNAYRDMGSS
                     YWMSNETFLKLKIKYGWDERYGFLDLNTGCKIPRIYRADICLTFMCSKLKKMLGPEKV
                     RMLNKLTSRILFARYGDQNNKMMKILDEEFV"
     gene            5922..6443
                     /locus_tag="P5293_05735"
     CDS             5922..6443
                     /locus_tag="P5293_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49437.1"
                     /translation="MSKEKREIRLVIIIIEQFHMKVVDGHRVMELTGLSPGPQVGKII
                     KKATEWAIDNNYGTYYTLGISFQTHRGKFFVPAEYSRQHGENEYDFGRKFYASNGILL
                     RSISYPEWKAPDTLYVRGKFINKDINTFIVSEMNANRIFEAVDEYNLTFGYSTFGYSD
                     AFPILDDNLFIIK"
     gene            6762..7130
                     /locus_tag="P5293_05740"
     CDS             6762..7130
                     /locus_tag="P5293_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49438.1"
                     /translation="MVNRNFVDEDRNKILVEAMDEDYIEREGKEITEIVSTLYEQDFS
                     TWDGFSHLLEWCKDQNWWEIFIGEHGIVLYKDENDSPNYYFPVDLLSPDKFADAVVEF
                     LEALKESDDDKPEEKDEWRT"
     gene            7132..7347
                     /locus_tag="P5293_05745"
     CDS             7132..7347
                     /locus_tag="P5293_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49439.1"
                     /translation="MFDKLKNSQLIEEVLDKISDEGLEEKQLKAVVYITIMAYNSGDI
                     QSLSEFIVEYLKGLKHMMEMGKQKVIH"
     gene            7347..7547
                     /locus_tag="P5293_05750"
     CDS             7347..7547
                     /locus_tag="P5293_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49440.1"
                     /translation="MKSKNSWRNFQNYCDMKIDRKRELRNRMSCLEKEELLIVLDKKL
                     PLYYNDFRSLSKKELIDLLCEV"
     gene            7549..>7865
                     /locus_tag="P5293_05755"
     CDS             7549..>7865
                     /locus_tag="P5293_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49441.1"
                     /translation="MGDFFDYVDRRKWYEKLWNRIYGFFYGIPMNIRWAFKLFTAFFV
                     RGRRGWAHIDTWNLDHYLARVIRDSIQNLKDTMPGVPSLVDTEEEWNKILDHIIWTFD
                     MAV"
BASE COUNT         2502 a         1388 c         1803 g         2172 t
ORIGIN      
        1 gataacctcg gcaggtagca agaatcccct gccttcaggc atggggagtg tcaaatcagg
       61 caagaggaaa ttcattccgg cggagtattg tgtgaatctt gatatattgt attttaaccc
      121 tcatttgttt aaaatagaat gatttcttcc atacagaatc cttgatcatt gggtaaacag
      181 agacttgagg ccattgacat tatagaaggt agagggaaag gtggatggaa aaatcatccg
      241 tatgtggcga aactaccccg agttgtatta caatgttatg ataaaataca atgcctcttt
      301 acgaaaagtc aaatggcctc gatggctggg tatctgtcac atcagatcaa tggaaagacc
      361 catggcatta cggtaaatac tggaaccata aaaaatacat aaatgcttac ggacaacaac
      421 tttttgttat ggacgaataa accactggat gaagtgtggg atggcatcga cacttccgtc
      481 aaggtcgatg atgaaagagg gttggggcca atgaaattaa agggtggcag gaataaagct
      541 gtagtatcat gaaaactgta gtatcatgaa agctgtggta aggataaagc tgtggcatta
      601 tggaagctgt ggtaaggata aagctgtgtg gcaggaataa agctgtggca ggaataaagc
      661 tgtggcatta tggaagctgg atataattta atcctcaatt acacacacaa agccccacga
      721 caatttttct gacctttaag taacatacaa ggaaaacaaa ttatactaaa accattctgc
      781 cataattcta ttcccgtaga atgacattat tatgcattct catataaggg agaagtgaga
      841 cacaaaattc cctgatacaa taatacgatt agagcgtcaa aattttgaca atatgtcaat
      901 atattgacac tttcggttac cggccgtttc tggaccggaa aaaatttcat tttttttata
      961 agttattgaa atcattgatg tatattttca gggagggaca aaaaaaccct tgactttatc
     1021 cctataatat gttaatatta ttatacaaaa tcaagaaagg tcagaaaatg ttctcaccga
     1081 aacctactta cagtacgacg gaatttggta agccccttct ttacaatttc aacaccaatt
     1141 caaaattgga agttcaggta acggtgaaac gcccctttgg tggtgtccgc agaaactgga
     1201 tttccgtcag ccccaatgtc ttccggtcct atcatggtaa gcgacgcgtg gacggcaagg
     1261 tcttcaaggg tgttgtctac tactggatga gcaaccagca gtatggttcg cctactacga
     1321 agaaagagcg ttcgacagtg aagatcaccg tgatccggaa gccccgcaag tccaagtccg
     1381 tgaaccttcg catcaagtta gccgaaagga gatagaaaat ggataagatg gaccttttga
     1441 ggaaagaaat cgttgagttc aggaacatct tggaaaagat caatgaggag tacatgtacg
     1501 gatctaagga tggatgggat ccggcggact actactccga tgttatgtac cgtgcctcga
     1561 ttttcttcgc ccgtatttcg gagcttgtat aatgatgaat gaattgattc agagaataca
     1621 gagaacaagg gatattatct atcgcaatgg tgatcttccc tgtatggcga ttgttcacaa
     1681 ccttcagaac tattcgacga tcctcaagga aatgggttta aacttcgaag ctgcggctgt
     1741 tctctctgcc gggtcactgt tgtacaattt ccccgtttac aagttcgatc agatttaccc
     1801 ggattcaatc cgttatgggg cggtcattct tcttgaccgt gttattgaca atctgacaga
     1861 aaggatgtaa aatgaatacc tactatgtag tgaccgagat tttcaatgtc cggtttatgt
     1921 atgggtccta taccaaagag agagctttct ggatggcacg gtttttaaac ggttttgttt
     1981 acattgaggt gaaacgataa tgtatctgaa gaataacctg aaattgaaag atggaacctt
     2041 ttatgtcaag ggaagtccgg tgtcgagatt tttcttcctt tccgacaggg tgactgtgat
     2101 cacgatcaat gacagggaca tcaggatcgc gactaccaga atgtacaaat atctgaaagg
     2161 tttcaccaaa ccaccgggaa aagccaccgt ctggaaatgg atgcacaatg aatgttgcaa
     2221 gactgtgacc ggaaagaaag tagagccgga cggaacggga ccggacggat caccttcatg
     2281 gttgagagtg ctggggttga tataatgatt tttaacgaaa tcgacagtgc cgaagatacc
     2341 cgtcttttct gtattctcga cggtatagtg accgcggcgg tccatctcat ccccgtggtc
     2401 gtcgctgtaa tgtcctttat atagagggag aaaaaaaatg aaaaagacat acaccaaaaa
     2461 cgaaatcatc gaaattctgt tgtttctcgc cgaaaaggca gacaaggacg ccctgagatt
     2521 cgaaaacaac gggaacatgg aatgccgaaa tatttccgag ggattacacc atgcctactt
     2581 aaccacagcc agctggctga aaaaaatcaa cataaaagaa taaaaatatg acatctcaac
     2641 atgtttcggc gttaaaaacc tttctccaaa agtcgattga aatggacgat gttttcgtcg
     2701 gttcagacaa ggaaaatctc ttgaaacgac ttcaagagga aatcaaaaaa atcaaggaag
     2761 ggaaaaaatg aataagttag catactacct tcgaatcgaa aatctggcga actatattgc
     2821 caagaaccta tctaccccta tggtggacga gaatggtgac accaaccttg tagtcgatat
     2881 atcggaagtc cacaggacgg cattcgagtg ggggttccac gatgacgata tgccggacat
     2941 tatcaagtct gtagaagaaa gggtttttat caattataag gaataagagg aaaaaatgat
     3001 tcaagaactc ggcattgcct acacattcga tcacaaggaa gggatcaaga aaaattgttt
     3061 ttccttcgta tacaagagat ataacatccc ttcgcaaatg aaaatttccg aaaccattta
     3121 tatcttggct gacaattgtc agcgcgccga gaataaactc tataggctca tcaatcactg
     3181 gaacaccctg tcatgggaat ggaaatttac tgtatagaaa aatgtcgaac aaatcacccg
     3241 aaatggaaaa gtttttgaac gatctttcca agatgttgta tggccgggaa cggcattccg
     3301 gcgcctgtgt cgcttgtggc tctcacaagg tctctgcaga ggattttcgc gatgctcttt
     3361 cctatacgga atacggcatt tccaatctct gccaagaatg tcaggacaaa atttataaaa
     3421 tatgccaaga gtaaaaacta aattgaacaa atatcaggtt ttcacccaga ccttcccgac
     3481 gccgaaagcc actaaggcat atttcaagct gcactatgcc aagggggcta aaatcgtggg
     3541 gcctctgact tggaaagtga aaaacgggaa ctatgtccgc ttgtaccttt tcggcatcga
     3601 ggagctcaag tccataaaac accttcagca gcctaagaca aagaaatatt aatatctgga
     3661 acagagtata taaaacccaa taaaaacaac actatataag gagaaaaaaa tgaaaaaagc
     3721 aaagatgaca ggcaaggaaa tccagaagca ggtatcagag gccagaaaac cctccacggt
     3781 ggaagctaag ggccagttca agttctacaa ggacaccaag cgctatcacg cctacaatat
     3841 aaccggcagc catggcatca ccggtgttgt ctacacgccc cgcggggagg atgtccccga
     3901 catcatcacc ttgaaattcg aaaagaagag ctgagagagg acttaacacc ccataggcgg
     3961 gggaaatttc ccgttcacct cccccgccta tatcaaactc ttgacacgca acagttttac
     4021 ttttattgac acaatctgtg tgctatggta taatatacat acagcataga ataaccgaaa
     4081 atatcaaaat tttgatgttc aaatcaatgt agaaaattat ttacccctta gtaattgagg
     4141 ggtaaaaata cggatctttg ttaagaaata cgcaagcttt ttgacaaggg tagtttccgc
     4201 ctccgcatta aaaaaatttt ggcggcccta aataaaaagg gtagtttccg ccttttggag
     4261 aaggggaaag atgaaataca cggaaaaaca attggctgat atgttctctg ctcttgctga
     4321 taagtattct gctctggctc aggaagcttt gaagaacaat gatttagaag attacaactg
     4381 gtacggtggg caatcctctg cttttcagag aacagcggat ttcttctgta aacttgataa
     4441 tatgaaggaa aaacggtgcg tgaacgagaa gaaaaccctt gaagagaatt gataaggggc
     4501 ccgcacgcgg ggcccggatc atccaccgtg aatgtgagtg gcggagaagg tatatatgga
     4561 acacgaaaga atgcgatttg ctacaatgac cccccggcaa ctccggacaa ggttgaaccg
     4621 gattacagat cgtgagaaat tgagaaggtt ccttatagtg tgtgctgaaa agataaggga
     4681 actggaatct gcgggtttgg tgcaccgagc tgctgattac cgagaccttc aggcggaagc
     4741 tcactggaaa ctctacggtt atacgacctt tggtgcggca aaagacaggg agcggatttt
     4801 ggatgatgat tatctgaaaa ggataggaat ataacagtaa ggttacaagg aaaagtacgg
     4861 cttttacatc aacgggtttc aatcatttcg aaagagattg acccgattat ctgtgcgttg
     4921 aaggtaataa atatggactg ttttactttg agttgaggtg ataatatgac agagtatata
     4981 caatttcttt tattgatact attcatgcat tgggtcacgg attttgtttt tcaatccgat
     5041 gcgatggctc tacagaaatc agagaacaat tgggtgctcg ggaaacattg tttggtctat
     5101 agtttaccga ttgccttccc gtttctgatt tttggtttac taaaggccct tgtggtgttt
     5161 gttgtatttt tccttacaca ttttagtatt gactatgtga cagcaaggtt gaacaaaaag
     5221 ttctggctga acaacaagag gcatgaattt tttgtgatga ttggtttcga ccagtttttg
     5281 catatcggta ttattttact tttgtcaagt ctattatgga gaggttaagt gaaaagggct
     5341 ttggagaaaa tatatcaaag aaccaggtga ctacaatata tcatagaata tgaagctcga
     5401 cagtcgtgat ctcgtcgcat tgattacaat gggatttgtc attggtgtgt ttttatcact
     5461 tgttttgtac aaggtcttat aatgaatttc tggcagagat atataagtga atgtcgaagg
     5521 atggatattc ttctgaaaga agcaacatcg gagctatgcc caaattgtga ttttcttttt
     5581 cacgaagcgg aagttatggg atgttgtggt ttctgtggtg atatgaacgc ataccgtgat
     5641 atgggatcat cctactggat gtcaaatgag accttcctga aattgaaaat aaaatatgga
     5701 tgggatgaaa ggtatggttt tcttgacttg aatacagggt gcaaaatccc ccgtatctac
     5761 agagcagata tctgcttgac atttatgtgt agtaagttga aaaagatgtt ggggccggag
     5821 aaggtcagga tgctcaataa gcttacatca agaattttat tcgcacgtta tggtgatcag
     5881 aacaataaga tgatgaaaat acttgatgag gaatttgtat aatgtcaaaa gagaaaagag
     5941 aaatacggtt ggttatcatt ataatagaac agttccatat gaaggtcgtg gacgggcaca
     6001 gggtaatgga gttgacgggt ctttcccccg gtccccaagt gggtaagatt atcaagaaag
     6061 ccacagaatg ggcgatagat aataattatg gtacatacta taccttgggt ataagttttc
     6121 agacccacag aggtaaattt tttgttccag cagaatattc caggcagcac ggggaaaacg
     6181 agtatgattt tggtaggaag ttttatgcct caaacgggat actattgagg tctattagtt
     6241 atccagagtg gaaagccccg gataccctct atgtaagagg aaaattcatt aacaaagaca
     6301 ttaatacctt cattgtttct gaaatgaacg ccaataggat atttgaggcg gtggatgagt
     6361 ataaccttac ctttggttac tctacctttg gttactctga tgcttttccg attttggatg
     6421 ataatctttt cattataaaa tagttattta ccggacctgt attttatggt ataattactc
     6481 catcgagagg tgttgaaatg tcttattctt atatttgtag aaaaaccaca tattgtaata
     6541 atgaaaattg tgaacatttt tcctctcaca aaagaagggt tgtttctgtc tctatgagaa
     6601 atatgcagct ggtgtactgc accagagtac attactgtcc ttcgacaggt ctttgggttt
     6661 gttgcggccg ggatcttccc cacaatgatg atttatacct tgaaagattg gggtacacaa
     6721 agaagagata ttccagaaat cgaacgtgaa aggataataa tatggtaaat cgtaattttg
     6781 ttgatgagga tagaaacaaa atccttgttg aggcgatgga tgaggattac atagaaagag
     6841 agggcaagga aattacagaa atagtgtcta ctctctatga gcaggatttc tccacttggg
     6901 atggttttag ccatctcctt gaatggtgta aagaccaaaa ttggtgggaa atttttatcg
     6961 gggagcacgg tattgttctc tataaggatg aaaatgactc acctaattat tactttccgg
     7021 ttgatttatt atctccggat aagtttgcag atgctgttgt cgagtttctt gaggctctca
     7081 aagaatctga tgatgataaa cccgaagaga aagatgaatg gagaacatga tatgtttgac
     7141 aaattaaaaa attcccagtt aatagaggaa gtacttgaca agataagtga tgaaggtctg
     7201 gaagaaaagc agttgaaagc cgttgtttac ataacaatta tggcttacaa ttccggcgat
     7261 atccaaagtc tatcggaatt tattgttgaa tatctgaagg gtttaaagca tatgatggag
     7321 atgggaaaac agaaggtgat tcactaatga aaagtaaaaa cagctggaga aactttcaaa
     7381 attattgtga tatgaagatt gatcgtaaga gggagcttcg gaaccggatg agctgtttgg
     7441 aaaaggaaga gcttctgatt gttctggaca aaaagctccc tctctactat aatgacttca
     7501 ggagcctctc aaaaaaagag ctaatagatt tactttgtga ggtatgatat gggtgatttt
     7561 tttgactatg ttgataggcg caaatggtat gaaaaattgt ggaataggat ttacggcttt
     7621 ttctacggga tacctatgaa catcaggtgg gcgtttaaac tctttacggc cttttttgtt
     7681 cgtggtagaa ggggctgggc ccatatagat acttggaacc ttgatcacta tcttgccaga
     7741 gttatcaggg atagtattca gaacttgaaa gatactatgc ccggtgttcc aagcttggtt
     7801 gatacagagg aggaatggaa taaaattctt gatcacatta tttggacatt tgatatggcc
     7861 gtcaa
//
LOCUS       DAOTQL010000081         7839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7869_length_7839_cov_4.779291, whole genome shotgun sequence.
ACCESSION   DAOTQL010000081 DAOTQL010000000
VERSION     DAOTQL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7869_length_7839_cov_4.779291"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1255)
                     /locus_tag="P5293_05760"
     CDS             complement(83..1255)
                     /locus_tag="P5293_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRR49442.1"
                     /translation="MRRLTVLLMILLLASILNAQNVSEQTARKQKIEEEIAYLDKQLE
                     ATKSKKQSNTKELAYIQRKIANRKKLLKEIENEIKTIDSEVAIKEQQIAELDKNLESL
                     KQSYSEMIYNAYKNRDQRLWIMYILASKNFEQGYKRWIYLRNYNETLKEMADEIRAHS
                     LQIAEERFQLAKIKNSSLSNQAKKELEVKRLTYDENNAKQVISQLSRKEKEVIQQLNV
                     KRKEVEKLNREIERILSDAAKEKERPDYKASAVDIALSEEFGKNKGKLPWPVKKGAII
                     EQFGQHNHPVFKNVKMPFNNGVNISTDAGADVSCVFDGVVKQVLMMPGYNQCILVQHG
                     DYFTFYTKLDAVYVKSGQKVKTGETLGKLAISDDESVIHFQLWKGTSKQDPEEWLR"
     gene            complement(1252..2844)
                     /locus_tag="P5293_05765"
     CDS             complement(1252..2844)
                     /locus_tag="P5293_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR49443.1"
                     /translation="MDGLREYNLGNPDKAFAIFSSLARKGTHNDAVYYYLSNLYQYRN
                     RPDSAVAMMEKALKLDPTNTWYSSQIASFYMANNQIDKAIDVYETLRKKYPQQIELYD
                     GLVDIYINRKDHENALKVLEDIEKYAGKSEISCLTRYNLYIFQNKRDEAIKYLTEYDK
                     DNGTPRTSTVLGDYYSSLKQDSLAMEYYAKALAMDPSYVPAIFGQAEINRIKNNFSPY
                     FRSMIPLMANPIVMPELKVDYLSQILSNQKFVELFFPQIDTLVENAYIAHPGDSSIAY
                     LYSRYLALTGRGEQAVNVLKDNLKYYGESRIAHREYLSLLYYMEEWDKIIEHVAQYSQ
                     LFSRDPSLLEIKGLAYVRKDMIPEAIAVYKNVLNLAKRDSSMEVRALSVLGDLSYQAG
                     NRKEAYKYYRKTLRIAPNYNPVLNNYAYYLSLEKRKLKLAYEMSKKTIDSEPDNPTYL
                     DTFAWILHLMGKDLEAKAIIKHALIYGGNESAAILDHYAEILYNLNEQDLAFRYWEQA
                     DKLDPSLGISEKVSRIKEGQKK"
     gene            3071..3622
                     /locus_tag="P5293_05770"
     CDS             3071..3622
                     /locus_tag="P5293_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRR49444.1"
                     /translation="MEANSRHFTDDRELLEGCLKQDRIAQKTLYEKYAPRMLSLCTRY
                     IGNKERAWDVLQDGFITVFEKIGSYNGTGSFEGWMRRIFVNTALMSLRKSDVLKFSEE
                     LATVEREVVTEQTALDGISSKELLRLIASMPEGFRVVFNMYVIEGYSHQEIAQIIHIS
                     EGSSRSQLSRARIWLQNRLKEKF"
     gene            3625..4830
                     /locus_tag="P5293_05775"
     CDS             3625..4830
                     /locus_tag="P5293_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRR49445.1"
                     /translation="MQNIDFDKRIREMMETHEETPPFDGWERINSALERKRAKIIHFR
                     RGIYIASAVAASLLLLLVINRNAPQKPGDIIKPQLFSERGRGSAQASNKAQTEQVSKN
                     IACVQISKSIERTLSATSEANAPGEKSVVPDEKSVALDEKSVVTDEKSVETRDKSIAN
                     NSTKPVKPYNFDEWLNSEDEKKSFRKWLSDRKYKYALSTNISSSYYKKSINLVSVSLG
                     YQNDFVPSSLQELVRSHYVSDIRYAMPVSFGIQFQTDIDKKFSLGSGLVYTLLSSNYQ
                     EISETYSRDVNQSLHYIGLPVNGYYNLFENRSVKLYLVAGVMLEKGIAASYKESTDGV
                     KKYHTESISGMQFSAGGGVGIEWKIAKDLGIYFDPSMSYFFKGKQPSSIRTAQPLQYK
                     FEMGMRFEI"
     gene            4830..5867
                     /locus_tag="P5293_05780"
     CDS             4830..5867
                     /locus_tag="P5293_05780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRR49446.1"
                     /translation="MTSIAIVILNWNGRHYLERFLPIVEERSNRQDTVIVVADNGSTD
                     LSVDYLKQHHPNVRLIEFDRNWGFTGGYNRALEQVDAEYFVLLNSDVEVTEGWLDPLV
                     AHFQNHSNTGICMPKILSAYNHQMFEYAGASGGFIDFFGYPFCRGRILTNIEEDRGQY
                     NDSIEIFWASGAAMMIRSSLYRQLGGFDEHFFAHMEEIDLCWRAKLTGNHVWVVPQSV
                     VYHVGGGTLPNNSPQKLYFNFRNNLMMLYKNLPSNCLVPVLLTRMMFDGGSAIVYLLQ
                     LKFSYLRSVLKAHRDFWRSLLSLQRSSISVEGNKRGKVRISGVYRGSIVVRFFLSFKK
                     LTYFKIGADIT"
     gene            complement(5879..7183)
                     /gene="murF"
                     /locus_tag="P5293_05785"
     CDS             complement(5879..7183)
                     /gene="murF"
                     /locus_tag="P5293_05785"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRR49447.1"
                     /translation="MDLEQIYQIFRKSTGVCTDSRVLKEGELFFALKGDNFDGNKFAH
                     KALEKGALYSIVDDKTVPSHDRIIVVDDVLRKLQELAHFHRKALDIPIIGITGTNGKT
                     TTKELVSSVLSTKYKTAFTKGNLNNHIGVPLTLLQMDESIQIGVVEMGASAPGEIASL
                     VEICEPNYGIVTSVGKAHLQGFGSLEGVKKTKGELYDFLKKHGGVIFFNADNPDIYAM
                     VKEREPVSCFPYGLRFDNAEIFPVTIDNPFLKIKFKNGKIVETHLIGSYNADNVMAAL
                     AVGKHFGTDTDASVEAIKNYLPTNNRSQLTEGKYNMLIVDAYNANPTSMKAALSSFRE
                     LNADRTALILGDMLELGEESQKEHREVLKITSDMNVEKLFFVGNEFATALPYFTDLSL
                     KSKTFLTSSELRDYLLLNPLNGYTVLIKGSRGIAVEKVIDVL"
     gene            complement(7196..7630)
                     /gene="dut"
                     /locus_tag="P5293_05790"
     CDS             complement(7196..7630)
                     /gene="dut"
                     /locus_tag="P5293_05790"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRR49448.1"
                     /translation="MEVKIINHSPFDLPSYATEHSAGMDLRANIEESLAIEPLKRAII
                     PTGIFIELPNGYEAQIRPRSGLAAKHGIGIVNSPGTIDSDYRGEIKIILVNLSDEPFI
                     LNPGERIAQMVISQFERIEWKTVDELEKSDRGNGGFGSTGKN"
     gene            complement(7612..>7839)
                     /locus_tag="P5293_05795"
     CDS             complement(7612..>7839)
                     /locus_tag="P5293_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49449.1"
                     /translation="MMLISIMLGNRYYPIPYKWGRVMSFVAVGLAIYGLTIFIPPLPL
                     ILKYTIHTILIVMYILYYLKLEKISVWKLKL"
BASE COUNT         2234 a         1464 c         1541 g         2600 t
ORIGIN      
        1 gcaaccgtcg gagatagcat ttatacctcc aacttctgtg ataagagaat ttataggggg
       61 cagtagcttt atttattgat tgttatctaa gccactcttc cgggtcttgt tttgaagtac
      121 ctttccaaag ctgaaaatgt atcactgatt cgtcatcgga aatggcgagt ttcccaagag
      181 tttcacctgt cttaactttt tgtccactct ttacgtaaac cgcatcgagt tttgtgtaaa
      241 aagtgaaata gtctccatgc tgtacaagta tgcattggtt atatccgggc atcatcaata
      301 cctgtttgac cactccgtcg aaaacacacg aaacgtcagc tccggcatcg gtagagatat
      361 taaccccatt gttaaaaggc attttgacat ttttaaagac aggatgatta tgctgtccaa
      421 actgttcaat aatagcgcct ttcttaacag gccatgggag tttcccttta tttttaccaa
      481 actcttcaga gagcgctata tctacagcag aagccttgta gtccggacgc tctttttcct
      541 tggcagcatc agaaagaatc ctttctatct cccgattcaa cttttcaacc tcttttctct
      601 ttacattaag ttgttggatc acttctttct cttttcttga gagttgcgat ataacttgct
      661 ttgcgttatt ttcatcgtat gtaagtcttt taacttcaag ttcttttttg gcctggttgg
      721 acaaagaaga attttttatt tttgcaagtt gaaatctctc ttctgcaatt tgaagactat
      781 gagccctaat ctcatcagcc atctctttaa gagtctcatt ataatttctc agatatatcc
      841 accttttata accctgctcg aagtttttac ttgccaggat gtacatgatc cagagtcttt
      901 gatctctgtt tttatatgca ttgtatatca tctctgaata actttgcttg agagattcga
      961 gatttttgtc aagctctgct atttgctgtt ccttaatagc tacttctgaa tcgatggttt
     1021 ttatctcatt ttcgatctct tttaacagtt tttttcggtt tgcaatcttc cgctgtatgt
     1081 aagcaagttc ttttgtgttt gactgttttt ttgattttgt ggcttcaagt tgcttgtcaa
     1141 gataagcaat ctcctcctca attttctgtt ttcttgcagt ttgctcactg acattctgag
     1201 cattcagaat ggatgcaagt aataatatca tgagaagtac agttagcctt ctcatttttt
     1261 ctgaccctct ttaatacgtg atactttttc agaaattccg agggaggggt ctagtttatc
     1321 agcttgttcc cagtatctaa aggcaagatc ctgttcgttg agattgtaaa gtatctctgc
     1381 ataatgatcg agaattgcag cactttcgtt gccgccgtaa ataagtgcat gtttaatgat
     1441 ggcttttgct tccagatctt tgcccataag atgcagtatc catgcaaatg tgtccagata
     1501 tgttggattg tcaggttctg aatctatggt ttttttgctc atctcatagg ccaatttgag
     1561 ctttctcttt tcaagactga gatagtatgc gtaattgttt agtacgggat tgtaattggg
     1621 agctatacgc aaagtttttc tgtaatattt atatgcttct ttacggtttc ctgcctgata
     1681 actcaagtcc cccagtactg aaagcgctct tacttccatg ctgctgtctc gtttggcaag
     1741 gttaaggaca tttttataga cagctatagc ttcaggaatc atatcttttc ttacataggc
     1801 caagcctttt atttccaaca gagaaggatc acgagaaaat agttgggaat actgagcaac
     1861 atgttcaatt attttatccc attcttccat ataatataga agagagagat attcgcggtg
     1921 tgcaattctc gattctccat aatatttcag attgtcttta agaacattta ctgcctgttc
     1981 gcccctgcct gtaagtgcaa gataacgtga ataaagatag gctatactac tgtcgcccgg
     2041 atgagcgatg taggcatttt ctacaagcgt gtctatctga ggaaagaata attcgacaaa
     2101 tttctgattg gataatatct gagagaggta atctaccttt aattcgggca taactatcgg
     2161 atttgccata agtggaatca tacttctaaa gtaagggcta aaattattct ttattctgtt
     2221 tatctcggct tggccaaata ttgcaggaac atatgaagga tccatagcaa gtgctttagc
     2281 ataatattcc atagcaagtg aatcttgttt cagtgatgaa tagtaatcac ctaaaacagt
     2341 tgaagttcta ggcgtgccat tatctttgtc atattcagta agatatttta tggcttcatc
     2401 gcgtttgttc tgaaatatgt ataaattata tcttgtcaga catgaaattt cacttttccc
     2461 ggcatatttc tcaatatctt ccagtacttt aagggcattt tcgtgatctt tgcgattgat
     2521 gtagatatca acaagtccat cataaagttc tatctgttga ggatattttt ttctaagagt
     2581 ttcgtaaacg tctatagcct tatcaatctg attgtttgcc atgtagaaag atgcgatttg
     2641 tgagctgtac caggtatttg tcgggtcgag ttttaatgct ttctccatca tcgcaactgc
     2701 gctgtcaggt ctgtttctat attgatataa attgcttaaa taataataaa cagcatcgtt
     2761 atgggtacct ttgcgtgcta atgaagagaa tattgcaaag gctttatccg ggtttcccaa
     2821 attatattct cttaatccgt ccatatagag catctcctca ttctcggttt tctgctgggc
     2881 attaaggtgc gtcaccagag agaatagtga aaaaagacat atggaaagaa tccttgcaat
     2941 atgtctcaaa tttaaaacat tgttcatttt ataatgcatg ataactcact tatgcaaaga
     3001 tagtgtttta taaattttta tttgctgtgc agcgaattat tactattttg catctaataa
     3061 aaacaagtga atggaggcca attcgcggca ttttacagat gatagggagt tgcttgaagg
     3121 ttgcctgaag caggacagaa tagcacaaaa gactctatat gagaagtatg caccacgaat
     3181 gctctctctt tgtactcgct atattgggaa taaggagcga gcatgggatg tcctccagga
     3241 tggttttatt acggtgtttg aaaagattgg cagctataat ggaacaggct cgtttgaggg
     3301 atggatgagg cggatttttg taaatacggc gttaatgtca ctcagaaagt ccgatgtact
     3361 taagttttct gaagagcttg ctacagttga aagagaggtt gttactgagc aaacggctct
     3421 ggatgggatt tcctctaaag agttgctcag attgatagct tcaatgcctg aaggattcag
     3481 ggttgtcttc aatatgtatg ttattgaagg ttactcccat caggaaattg cacagataat
     3541 tcatatttct gaaggcagtt caaggtcaca gttgagtagg gcaaggatct ggctacagaa
     3601 caggttgaag gagaaatttt aaatatgcag aatattgatt ttgataagag aatcagagaa
     3661 atgatggaga ctcatgaaga gactccaccg tttgatggtt gggagaggat caattctgct
     3721 ctggagagaa aaagagcaaa aataatccac ttcaggagag gtatatacat cgcctctgct
     3781 gtagctgcat ctttattgct tttgcttgtt ataaacagaa atgctcctca gaaacccgga
     3841 gatatcataa agcctcagct attttcagag agagggagag gcagtgctca ggcaagcaat
     3901 aaagcgcaaa ccgaacaagt ttcaaaaaat attgcatgtg ttcaaatttc taaaagtatt
     3961 gaaagaacgc tgtccgctac atcagaagca aatgcgccag gtgaaaaatc tgtggtacca
     4021 gatgaaaaat ccgtagcatt agatgaaaaa tccgtagtca ctgatgaaaa atctgtggag
     4081 accagggaca aatcaattgc aaataattca actaaaccgg taaagccata taattttgac
     4141 gaatggctga acagtgagga tgaaaaaaag agttttagaa agtggctaag cgacagaaaa
     4201 tataaatatg cactttcaac aaacatctca tcttcatact ataaaaagtc cattaatctg
     4261 gtttctgttt ctctgggcta ccagaacgac tttgttcctt ccagccttca ggaactggta
     4321 agaagtcact atgtttcaga catacgttat gcaatgccgg tatcttttgg gatacaattc
     4381 cagacagata tcgacaaaaa attttcttta ggttcaggtc ttgtatatac ccttttatct
     4441 tccaattacc aggaaatttc tgaaacttac agcagagatg ttaatcagtc tttacattat
     4501 atcgggcttc ccgttaacgg ctattacaat ttgtttgaaa acaggtctgt aaaactgtat
     4561 ctggttgcgg gagttatgtt agaaaaggga atagctgcca gttacaagga aagtacagat
     4621 ggagtcaaaa aatatcatac cgagtccatc tcaggtatgc aattttctgc aggcggagga
     4681 gttggtatag aatggaagat tgccaaggat cttgggattt atttcgatcc atccatgtcc
     4741 tactttttta aggggaaaca gccatcaagt atcagaacgg ctcaaccttt gcagtacaaa
     4801 ttcgagatgg gtatgagatt tgaaatttga tgacatctat agctattgtc atacttaact
     4861 ggaacggaag acattatttg gagcgttttc tgccgatagt tgaagaacgt tcaaacaggc
     4921 aagatactgt cattgttgtg gccgataatg gttctacaga cttatctgtc gattatctta
     4981 aacaacatca tcccaatgtc agattaatag agttcgaccg aaactgggga ttcacaggtg
     5041 gttataacag agctttggag caagtagatg cagagtattt tgttcttttg aattcagatg
     5101 tggaagttac tgaaggatgg ctggatcctc ttgtggccca ttttcagaat cattctaata
     5161 ctggaatatg tatgcccaaa attctatctg cctacaatca tcagatgttt gaatatgctg
     5221 gtgcaagtgg aggatttatt gattttttcg gctatccttt ttgcagaggg agaatattga
     5281 caaatattga agaagataga gggcagtata atgacagcat tgaaatattc tgggcgtcag
     5341 gcgctgctat gatgattcgt tcctctcttt atcgtcaatt gggtggtttt gacgaacatt
     5401 tttttgcaca catggaagag atagaccttt gctggcgagc aaagctaact ggtaatcatg
     5461 tctgggttgt tcctcaatca gttgtctatc atgtgggggg cggaaccttg cctaacaatt
     5521 cacctcagaa actctatttt aacttcagga acaatctaat gatgctttat aaaaatcttc
     5581 cttcaaactg tctggttcct gttcttctta cgagaatgat gtttgacgga ggctcagcta
     5641 tcgtatatct cttacagctc aaatttagct acttaagatc ggtgctcaag gcacatagag
     5701 atttctggag atctctcctt tctttacaga gaagcagcat atcagttgaa ggcaacaaga
     5761 gaggcaaagt gagaattagc ggagtttaca gaggaagtat cgtggttaga ttttttctct
     5821 ctttcaaaaa acttacatat ttcaaaattg gtgctgatat aacctgatta ttcttttctc
     5881 ataagacatc tataaccttt tcaacggcta ttccccgaga acctttaatg aggacagtat
     5941 atccgtttaa agggtttaaa agcagataat cccttagttc ggaggaagtt aagaaagttt
     6001 ttgattttaa agacaagtct gtaaaatatg gcagtgccgt tgcaaattca ttgcctacaa
     6061 aaaacagctt ttctacattc atgtcacttg ttattttaag aacttcccgg tgctcttttt
     6121 gtgactcctc accaagttcc agcatatcac ccaaaataag cgcagtacga tctgcattca
     6181 attctctaaa actgctcagt gcagccttca tgcttgtagg attagcgtta taggcatcta
     6241 ctatcagcat gttgtattta ccttcagtta attgagaacg gttgtttgtg gggagataat
     6301 ttttaattgc ctcgacagag gcatctgtat ctgtaccaaa atgttttcca acagcaagag
     6361 ctgccattac attatcggca ttataacttc caatgagatg tgtttctaca attttcccgt
     6421 ttttaaattt aattttgagg aatggattgt caattgtgac aggaaatatc tcggcattgt
     6481 cgaatctcaa gccataggga aagcaactta caggctctct ctctttgacc atggcataga
     6541 tgtcgggatt gtcggcatta aaaaagataa ctcctccatg tttcttcagg aagtcataaa
     6601 gttcaccctt agttttcttc acaccttcta atgagccaaa tccctgaagg tgtgcttttc
     6661 ctacacttgt tactattcca tagttgggtt cacatatttc aactaaagaa gcgatttccc
     6721 ccggggcact tgcacccatt tccacaactc cgatttgaat actttcatcc atttgaagaa
     6781 gagtcagagg tacaccaata tggttattga gattgccttt tgtgaaggca gttttgtatt
     6841 tagtggagag cacactgctc acaagttcct tggtggtggt tttcccatta gtcccggtga
     6901 ttcctataat ggggatgtca agagctttgc ggtgaaaatg agcaagctcc tgcaattttc
     6961 tcaacacgtc gtcaactact attattctgt catgtgatgg aacagttttg tcatcaacta
     7021 ttgaataaag agcacctttt tctaaagcct tatgggcaaa tttattgcca tcaaaattat
     7081 ctcctttgag agcaaagaaa agttcaccct ctttcaagac tctactgtct gtacacaccc
     7141 cagtactttt cctgaagatt tgatagatct gttctaagtc catttttatc tttctctaat
     7201 tttttcccgt agatccaaat cctccattac cacggtctga tttctcaagt tcatcaacag
     7261 ttttccattc aattctttca aattgtgata tgaccatctg tgctattctt tctccagggt
     7321 tgagtataaa aggctcatct gagagattga caaggattat ctttatctct cctctatagt
     7381 cggagtcaat tgtccccggg ctgttaacaa tgcctatacc gtgtttggct gccaatccgc
     7441 ttcttggtct tatttgcgct tcgtagccgt taggtaattc tatgaatata ccggtaggta
     7501 taattgcccg ttttaaaggc tctatagcca agctctcttc tatatttgcc cttagatcca
     7561 tgcctgcaga gtgttctgta gcataagaag gcagatcaaa cggagagtga tttataattt
     7621 taacttccat acagatatct tttcgagttt taaatagtaa agtatgtaca tcacaattaa
     7681 aatagtgtga atagtgtatt ttagaattaa cggcaaaggt gggataaata ttgttaaacc
     7741 atatattgca agtccgacag caacaaaact cataactctg ccccatttat aaggaatggg
     7801 ataatatctg tttccaagca ttatacttat taacatcat
//
LOCUS       DAOTQL010000082         7812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_7902_length_7812_cov_4.317004, whole genome shotgun sequence.
ACCESSION   DAOTQL010000082 DAOTQL010000000
VERSION     DAOTQL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7902_length_7812_cov_4.317004"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1149)
                     /gene="rlmN"
                     /locus_tag="P5293_05800"
     CDS             complement(130..1149)
                     /gene="rlmN"
                     /locus_tag="P5293_05800"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRR49450.1"
                     /translation="MKTWLLGKSLDQLREIVVNMSLPAFSAKQIAEWIYKKNCKDIMQ
                     MSNISKANRDRLSEQYQVGGFSPEKVTLSSDGTKKYLFPSLCETFIESVTIPEDDRVT
                     LCVSSQAGCRMGCKFCMTARVGYKGNLTAGEIISQLQNVEESDKITNIVFMGMGEPLD
                     NFGEVSGAINILTADWGFAWSPKRITVSSIGIHEPLIDFLNDYKCHIAISLHNPFEEE
                     RLEFVPAQKAWPFARTLEIIRRFDFTGQRRVSFEYIMFDGWNDSKRHADALIRTLKGL
                     ECRVNLIRFHQIPDFPYKPTPNAKIELFRERLNKEGLIATIRASRGEDILAACGMLSG
                     KNINK"
     gene            1215..2249
                     /gene="recA"
                     /locus_tag="P5293_05805"
     CDS             1215..2249
                     /gene="recA"
                     /locus_tag="P5293_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRR49451.1"
                     /translation="MAKESEEVKKGAEISSEKQKALQATLDKLEKDYGKGTVMKMGDQ
                     PKWEVSTIPSGSIALDHALGIGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKSGGIA
                     AIIDAEHAFDRTYAKNLGVNVDTLLISQPDNGEQALEIADALIRSGAIDIIVIDSVAA
                     LTPKAEIEGEMGDSKMGLQARLMSQALRKLTANISKTNTCCIFINQLREKIGVMFGNP
                     ETTTGGNALKFYASVRVDVRKVTQLKDGEETTGNRTRAKIVKNKLAPPFRKAEFDIVF
                     GEGISKVGEIIDLGVDLDIIHKSGSWFSYNDTKIGQGRDAVRQILLDNPALMEEIEAK
                     VREKLSQVKD"
     gene            2267..3256
                     /gene="trpS"
                     /locus_tag="P5293_05810"
     CDS             2267..3256
                     /gene="trpS"
                     /locus_tag="P5293_05810"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRR49452.1"
                     /translation="MEKVLSGIRSTGHLHLGNYFGALKNYIKMQNEYDCFFFIADLHS
                     LTTHPHPADLHSGVRTILAEYLAAGLDPEKSALFVQSDVPEVCELYVLLNMIAYKGEL
                     ERTTSFKEKVRKQEDNVNAGLLTYPVLMAADILVHRANKVPVGKDQEQHLEIARTLAR
                     RFNNLYGVNLFPEPQAFNFGDDLVKVPGLDGSGKMGKSEGNGIYLFDDEKTIYKKVMK
                     AVTDSGPTTPNSEMSEPVKNIFTLLKIVSTADTVNYFTEAYNNCTIRYGDLKKQLAED
                     ICKHTLPIRERIEEIYNDTDYLGKVARSGRDKARESAALTMKEVRAAVGIKSF"
     gene            3263..5221
                     /gene="dnaG"
                     /locus_tag="P5293_05815"
     CDS             3263..5221
                     /gene="dnaG"
                     /locus_tag="P5293_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRR49453.1"
                     /translation="MISKSTIDKILDTAQIVDVINDFVTLKKRGANYIACCPFHNEKT
                     PSFSVSPSKGIFKCFGCGKAGNVVNFIMEHEQLGYVEALKYLGRKYGIEVTDQEESAE
                     EQQNRLKSESLMVVSQFASEFYSNTLWNSPSGQAIGLTYFKERGFSEKTIRKFMLGWA
                     PSERDALSNAAIRTGYKKEYLVSAGLSIETENGKLFDRFYERVIFPIHSVSGKVIAFG
                     GRTLKTDKSTAKYINSPESEIYVKSKSLYGIFFAKSAISKFQNCYLVEGYTDVLSLHQ
                     AGVENVVASSGTSLTSDQIRLIRRFSPAVTILYDGDAAGIKASIRGIDMLLEEGLQVK
                     VVLFPDGEDPDSFARNHSPEELKEFLKDSEQDFITFKSNLLAEEARQDPLKKVQLISE
                     VVASIAVIPDAISRSVYIEDCSRRLNIDEKVLVQEVRRIRQKKQYGVYSQTLPSEERR
                     YAPEYIPVIRTGEKVPEFVTETFCEPAEKELLYYLLKFGEYPLYGIEEGKDRGITVSQ
                     FILTELQNDDLELKNLVYKNMFNEYFIVKELGQESIQKHFLNHENHEFVQVALELLHQ
                     EHTLNIKQLTKTLIPEENVLSQSVPKAILVYKAKITSLAYKKLCEELEKGQNEGASAD
                     EQNEVLRKLQILMHIRNSLSKELKRLNI"
     gene            5265..7325
                     /gene="metG"
                     /locus_tag="P5293_05820"
     CDS             5265..7325
                     /gene="metG"
                     /locus_tag="P5293_05820"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRR49454.1"
                     /translation="MKKSFKRHLVTAALPYANGPVHIGHLAGVYVPADIYVRYLRMRG
                     EDVRFVCGSDEHGIPITIKARQEGVSPQQIVDKYHEIIKKSFEGLGISFDIYSRTTSK
                     THYKTASAFFKKLYDEGKFIEKKSEQFFDEEAGQFLADRYIIGTCPKCGAENAYGDQC
                     EKCGCTLSPNELINPRSALSGNPPVMKLTKHWYLPLDQYESWLKEWILEGHKEWKSNV
                     YGQCKSWLDGGLQPRAVSRDLDWGVPVPVEGVEGKVLYVWFDAPIGYISNTIELMPDE
                     WEKWWKSEDTKMVHFIGKDNIVFHCIMFPAMLKAYGDGFILPENVPANEFLNLEGNKI
                     STSRNWAVWLNEYLEELPGKEDVLRYVLCANAPETKDNDFTWKDFQTRNNSELVAIFG
                     NFVNRSVVLTHKYFGGKVPAIYTLTPLDTEALAQIPQIKKAIEENLENFKFREALKEA
                     MNLARLGNKYLTDTEPWKVAKTDMDRVATILNVSLQICASLSIALEPFMPHSTLKLSQ
                     MLNIGQLKWDKLGSDAILSVGHQLNEAKLLFDKIEDDVIAAQIAKLEATKAANKKSTA
                     VNISSPEVEPQKEECSIEDFMKMDIRTATVLEAERVPKTDKLLKLTIDTGIDIRTIVS
                     GIAEYYTSEVMVGKQICILANLTPRKIKGIESKGMILMAKEKNGKMRIVSPEEVVANG
                     SMIG"
     gene            complement(7370..>7812)
                     /locus_tag="P5293_05825"
     CDS             complement(7370..>7812)
                     /locus_tag="P5293_05825"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49455.1"
                     /translation="LLENLYADEADITLEEAANEIESFIKDLRSDLNRLKSIQLPGFG
                     TMKATDKNDYFFVADKDLFIYPEAFGLEPLNIKILPKRGSLEILKKTSAKPKKKHRLG
                     LILLIILVILIIIIALMVFFKEEIRPVWEWILYSREERELLKLL"
BASE COUNT         2536 a         1502 c         1627 g         2147 t
ORIGIN      
        1 aaagaaataa tttccaaaga ggatctctct ttggaggagt aagcacattg ctgccgaatt
       61 tttttctgct ttcctcaact tcacttatac tgagacctga atgtaattta cttgtatcca
      121 tatattgatt cacttgttaa tgtttttacc acttaacatc ccgcaagccg caagaatatc
      181 ctcacctctc gaagcacgaa tcgttgcgat caagccttct ttgttcaatc tttccctgaa
      241 caactcaatt ttggcatttg gagttggttt gtatggaaaa tccgggattt gatgaaagcg
      301 tattagattt accctacact caagtccttt caaagtcctt atcaacgcat cggcatgacg
      361 tttcgaatcg ttccatccat caaacatgat atattcaaag cttactcttc tctgccccgt
      421 gaagtcaaac cgacgaatta tctcaagcgt ccttgcaaaa ggccaggcct tttgcgcagg
      481 tacaaattcg agacgttcct cttcaaacgg gttatgtaag gatatcgcaa tatgacattt
      541 gtaatcattc aaaaagtcga taagtggctc gtgaatacct attgaagaaa cagtgattct
      601 tttagggctc catgcaaatc cccagtcagc agttaaaatg tttattgcac cggatacttc
      661 accaaagttg tcaagcggct ctcccatacc cataaatact atattggtga ttttatcgct
      721 ttcctcaaca ttttgtaatt gagaaataat ctcaccagca gtgagattac ccttgtatcc
      781 cactcttgca gtcatgcaaa acttgcatcc catcctacag cctgcctggg acgagacaca
      841 taaagtaact ctatcgtctt caggtattgt gactgattca ataaatgttt cacaaagtga
      901 gggaaaaaga tatttttttg ttccgtccga agagagtgta accttttcag gactgaatcc
      961 gccaacttga tactgttccg aaagtctatc tctgtttgct ttcgaaatat tggacatctg
     1021 cattatatcc ttacaatttt tcttgtaaat ccactcagca atctgcttag cagaaaaagc
     1081 cggaagtgac atatttacaa cgatttctct gagctgatcc agactcttac caagtagcca
     1141 tgttttcatt ttacaaatgt agagattttt ggaaatcatt tgttattttt gcatgaacaa
     1201 aaaaatacaa cattatggca aaggagagtg aagaagttaa gaaaggtgct gagattagtt
     1261 ctgagaagca aaaagcctta caggctactc tggacaaact tgagaaggat tacgggaaag
     1321 gcactgtaat gaagatggga gatcagccaa aatgggaggt ttccacaata ccgtcaggat
     1381 cgattgcctt ggatcatgcg cttggtattg gaggataccc aagaggaaga gtcatcgaaa
     1441 tttatggccc tgagtcaagc ggtaaaacta cacttgcaat tcatgcaata gccgaagcac
     1501 agaagagtgg aggtatagct gcaatcattg atgcagaaca cgcatttgac aggacatatg
     1561 ccaaaaatct cggagtaaat gtggatacac ttttaatatc acagcctgac aacggagaac
     1621 aggctcttga gattgcagat gcacttatcc gctctggagc aatagacatt attgtcatcg
     1681 actcggttgc ggctcttact cccaaagctg aaattgaagg cgagatggga gactccaaaa
     1741 tgggtttgca agccagattg atgagtcagg ctcttagaaa gttaactgca aatatcagca
     1801 aaaccaacac atgctgcatt ttcataaacc agcttcgtga aaaaatcggg gtaatgtttg
     1861 gaaaccctga aacaaccaca ggtggtaatg ctcttaaatt ttatgcttca gttagagtag
     1921 atgtacgaaa ggttactcag ctaaaggacg gtgaggaaac aacaggcaac cgcacacgtg
     1981 ccaaaattgt caaaaataag ctggctcctc ctttcagaaa agcagagttc gacatagttt
     2041 tcggggaagg aatttcaaaa gttggagaga taatagatct cggagttgac ctcgatatca
     2101 tacataaaag cggttcctgg ttcagttaca atgacactaa aataggacag ggaagagatg
     2161 ctgtgcgcca gattcttttg gacaatccag ctttgatgga agagatagag gcaaaggtta
     2221 gagaaaaatt atctcaagtt aaggattaaa gtaattaaat gttttcatgg agaaagtatt
     2281 aagcggtata agatctacag gacatcttca tcttggcaat tatttcgggg cattgaaaaa
     2341 ctacatcaaa atgcagaatg aatatgactg ttttttcttc atagccgacc ttcattcctt
     2401 aactacacat ccacaccccg ctgatttaca ctcgggagta agaactattc tggccgaata
     2461 tctggcagct ggacttgacc ccgagaaatc tgctcttttt gttcaaagcg atgtgccgga
     2521 ggtatgtgag ttgtatgtgc ttctaaatat gatagcatat aaaggagagc ttgaacgtac
     2581 tacttctttc aaggaaaaag tgcgcaaaca agaagataat gtcaacgccg gacttctaac
     2641 ttatcctgta ttgatggctg ccgatatact agttcacaga gctaacaaag tgccggttgg
     2701 caaggaccag gagcagcacc ttgagattgc aagaactttg gctcgaagat ttaacaacct
     2761 ttatggagta aatttattcc ctgagcctca agcattcaat tttggggatg accttgtcaa
     2821 agtgccggga ttggatggat caggcaaaat gggcaaaagt gaaggaaatg gcatctatct
     2881 ttttgatgat gagaagacga tttacaaaaa ggttatgaag gccgttaccg actcgggacc
     2941 aacaacaccg aacagtgaaa tgtctgaacc ggtgaagaat atatttacac ttctgaaaat
     3001 tgtatccact gctgatactg tgaactattt tacagaagca tacaacaatt gcacaattag
     3061 atatggagat ctgaaaaaac agcttgctga agacatctgt aagcacaccc tccccatcag
     3121 agaacgcatt gaagagattt acaacgatac cgactatctt ggaaaagttg caagatccgg
     3181 tcgtgataag gcaagagaga gtgccgcttt aacgatgaaa gaagtcaggg cagctgtcgg
     3241 catcaagagc ttttaagcac tgatgataag caaaagcaca atagacaaaa ttctggacac
     3301 tgcacagatt gttgatgtaa tcaacgattt cgttaccctt aagaaaagag gtgcgaatta
     3361 tattgcttgt tgtccttttc acaatgaaaa gacaccttcg ttctcagtat caccttctaa
     3421 aggtattttc aagtgttttg gttgcggcaa agccggcaat gtggtcaatt tcataatgga
     3481 acacgagcag cttggctatg ttgaggcatt aaaatatctc ggaagaaaat atggcattga
     3541 agtaactgac caagaggaaa gtgctgaaga acagcaaaat cgtcttaaaa gcgaaagctt
     3601 gatggtggtc tcacaatttg caagtgaatt ttactccaat actctatgga attctccttc
     3661 aggtcaagct atcggcctga catatttcaa agagagaggt ttcagcgaaa agactatcag
     3721 aaaattcatg ctcggctggg cgccttctga aagagatgct ttaagcaatg ctgcaatcag
     3781 aacaggttat aaaaaggagt atttggtctc cgcaggtctc tccattgaaa ctgaaaatgg
     3841 gaaacttttc gacagatttt acgaaagagt cattttcccc atacattctg tttcaggaaa
     3901 agtgatagca tttggaggac gcactcttaa aactgacaag tcgacagcaa aatatatcaa
     3961 ttctcctgaa agtgaaattt atgttaaaag taaatctctc tatgggattt tttttgcaaa
     4021 aagtgctatt tccaaatttc aaaattgcta tctcgttgaa ggttatacag atgtcttatc
     4081 actacatcaa gccggagttg aaaatgttgt agcatcgagc ggaacctctt tgacaagtga
     4141 ccagattcgg cttatcagga ggttttcacc tgctgtaacc atactgtatg acggagatgc
     4201 tgcaggtata aaagcctcta tcagggggat agacatgctt ctcgaagaag gtttgcaggt
     4261 aaaggtagtg ctctttcccg acggagaaga cccagactct tttgccagaa accattctcc
     4321 ggaagagctt aaagagtttc tcaaagactc cgaacaggat tttattactt ttaaaagcaa
     4381 tctcctggca gaagaagcaa ggcaagatcc tttaaaaaaa gttcaactta tctccgaagt
     4441 tgttgcaagt atagcagtta ttcctgacgc aataagccgt tcggtatata tagaagattg
     4501 cagcaggcga ctaaacattg atgaaaaggt actagttcaa gaagttagga gaatcagaca
     4561 gaaaaaacaa tatggagttt attctcaaac acttccaagt gaagagagac ggtatgctcc
     4621 ggaatatatt ccagtcataa ggactggtga aaaagttcct gaatttgtaa ctgaaacttt
     4681 ctgtgaaccc gcagaaaagg aacttttata ttatctgctg aaattcggag agtatccact
     4741 ttatggaatt gaagagggta aagacagggg gataactgta tcacagttca tactcacaga
     4801 gttgcagaat gacgatctcg aactcaagaa tcttgtctac aaaaacatgt tcaatgaata
     4861 ttttatcgtc aaagaattgg gacaagaatc catacagaag catttcctga atcatgaaaa
     4921 tcatgagttc gtccaagttg ctcttgagct tctccatcaa gaacatacat taaatatcaa
     4981 acaattaaca aaaaccttaa tccccgaaga aaacgtactg agtcagagtg taccaaaagc
     5041 tattcttgta tacaaagcca aaatcacatc tctggcttac aaaaagttat gtgaagaact
     5101 cgaaaaaggc caaaatgagg gggcatctgc agatgaacaa aacgaagtac tgagaaagct
     5161 ccaaatactt atgcatatcc gcaactctct ctccaaagaa ctcaaacggc ttaatatttg
     5221 acattttttt tacctttgcc gactaataaa atatattcct taaaatgaaa aagagcttta
     5281 aaagacatct tgttactgct gcgcttccat acgctaacgg tcctgttcac attggtcatc
     5341 ttgcaggtgt atatgtgcca gctgatattt atgtgaggta tcttcgaatg cgtggtgagg
     5401 atgttagatt tgtctgtggc tctgatgaac acggtattcc aattaccatt aaggcgagac
     5461 aggaaggtgt ttctccacaa cagattgtag ataagtacca tgaaatcatc aagaaatctt
     5521 tcgaaggtct cggtattagt tttgacatat actccagaac aacttctaaa acacattaca
     5581 agactgcatc agcttttttt aaaaaacttt atgatgaggg aaaatttata gaaaaaaaga
     5641 gtgaacagtt ttttgatgaa gaagcgggac agttccttgc cgatagatat attattggaa
     5701 catgcccgaa gtgtggtgca gaaaacgcat atggggacca atgcgaaaag tgtggctgta
     5761 cactgagccc caacgaactg ataaatccca gatctgcact aagtggtaat cctcctgtaa
     5821 tgaagctcac aaagcactgg tatcttcctc tcgaccaata tgaatcatgg ctcaaagagt
     5881 ggatacttga aggtcacaaa gagtggaaaa gcaatgtcta cggtcagtgc aagtcctggc
     5941 tcgatggggg attgcaacca agagctgtca gcagggatct tgactggggt gtcccggttc
     6001 ccgttgaagg agtagaagga aaggttctct atgtatggtt tgacgctcct attggatata
     6061 tatcaaatac catagagtta atgcctgatg agtgggaaaa gtggtggaaa agtgaagaca
     6121 ctaaaatggt tcactttatt ggtaaggaca atatcgtgtt tcactgtata atgtttccgg
     6181 caatgctgaa agcatacggt gatggattta tccttcccga aaatgttcct gcaaatgagt
     6241 tccttaatct cgaaggcaat aaaatatcca catcccgcaa ttgggcagta tggcttaacg
     6301 aatatctcga agagcttccc ggcaaggaag acgttctgag atatgtatta tgtgcaaatg
     6361 ctcccgagac taaggacaac gactttacct ggaaagattt ccagaccagg aacaatagcg
     6421 agttggtcgc aatttttggc aattttgtca atcgttcagt agttttgact cataaatatt
     6481 tcggaggcaa agtgcctgct atctatactt taactccttt ggacacagag gctcttgctc
     6541 aaattccaca aataaaaaag gctatcgagg aaaatctgga gaatttcaag tttcgcgaag
     6601 ccttgaaaga ggctatgaac ctggcaagat tgggaaacaa atacctgaca gatactgaac
     6661 cttggaaagt tgccaaaaca gatatggata gagtggcaac tattctgaac gtttcattgc
     6721 aaatatgtgc cagtctgtct atagcattag aaccgttcat gcctcattca acacttaaac
     6781 tgtctcaaat gctgaatatc ggacagctta agtgggacaa gcttggcagt gacgccattt
     6841 tgtctgtcgg acatcaatta aatgaagcta agcttttatt tgataaaatc gaagatgatg
     6901 tgatagctgc acaaattgca aaacttgaag ctacaaaggc tgctaataaa aagtctacag
     6961 cagtcaacat atcctctcca gaagttgaac ctcaaaaaga ggaatgttct attgaggatt
     7021 ttatgaagat ggacattaga actgccacag tcctcgaagc tgaaagagtc ccgaaaactg
     7081 acaaactcct taaactcact attgacaccg gtatagatat caggacaata gtgtccggta
     7141 ttgctgaata ttatacctca gaagtgatgg taggaaagca gatctgcatc cttgcaaatc
     7201 ttactcctcg aaagataaaa ggcattgagt caaaggggat gattctgatg gcaaaagaaa
     7261 agaacggcaa gatgaggata gtatcacccg aagaggtagt cgcaaatgga agcatgattg
     7321 gataaatgat ttgaaatata acgatctgga tccgattaac aaattaatgt taaaggagtt
     7381 ttaacagttc tctttcctct cttgaataga gaatccactc ccaaacaggt cttatttctt
     7441 ccttgaagaa caccattaaa gctataatta ttatcaatat caccaggata atgagtaaaa
     7501 ttaagcccaa gcgatgtttc ttttttggct tggctgatgt ttttttaaga atttccaaac
     7561 ttccgcgttt aggaagtatt ttaatattta gtggctcaag cccaaaagcc tccggatata
     7621 taaaaagatc tttatcagcc acaaaaaaat aatcgttctt atctgtcgct ttcatagtgc
     7681 caaaaccagg tagctgaatg cttttcagtc tgttaagatc agacctgaga tccttaataa
     7741 aggattcaat ctcattggcc gcctcctcca aggttatatc agcctcatca gcatagaggt
     7801 tttcaagaag tt
//
LOCUS       DAOTQL010000083         7488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8309_length_7488_cov_5.816225, whole genome shotgun sequence.
ACCESSION   DAOTQL010000083 DAOTQL010000000
VERSION     DAOTQL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8309_length_7488_cov_5.816225"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..305
                     /locus_tag="P5293_05830"
     CDS             39..305
                     /locus_tag="P5293_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49456.1"
                     /translation="MDRDDVWNGAEERSAEFTSETCALVNNASIPLTPGSQFKATVLQ
                     LAKDAGFGKFRIVLNGSEIKPSQAPEVINEGDKIQITPYDVAGF"
     gene            374..1255
                     /locus_tag="P5293_05835"
     CDS             374..1255
                     /locus_tag="P5293_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49457.1"
                     /translation="MTTYTVKKEASEHLLNEMSNVFATIDSININDIELKGLSIRTGD
                     KLLKLKVVEVESLDPIEELKREYKNILNQKLAAIKDTIQKKVSDMVSFVQVYREEFER
                     KEIELKKKLESQQIMPPISFQQAERGLSVSPGQRNGEIHWFVRSVYWPKFVDQKPIET
                     KFQKKLITPIVILITTEGDHVKMVSTRTPIGLRYFSHYHQALPDCWGKWKWKPKWESP
                     EDILAIAKEVEGVLENINTASIANRSPVGLPTLNILSKHIVGRKEDLKKRDVNTTNMR
                     AGVSTSTNIVIEDSWST"
     gene            1259..3463
                     /locus_tag="P5293_05840"
     CDS             1259..3463
                     /locus_tag="P5293_05840"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HRR49458.1"
                     /translation="MNLQVIQKPASINILSYVGDTQGCGTIRVIYPHLLLPHLRYHGY
                     KFVGFYSAYFIRQADYYRNFSFVIFQRAATKSHLALISNFINQVTKTYKTPIIYEIDD
                     LLINIPEWNYASDYYKENEGHIEEIMRKVNGITTSTEKLKEVYSKYNSNIEVIPNHLP
                     KFVWGEIRPKHENYPRTEKERPRILWAGSENHFAVKPDKTGGDFGDELLKYIRGTVEK
                     YQWVICGGIPKELKDLKGKIEYHGWRNIFEYPSFLKSLDVDIAVAPLMQDIFNECKCL
                     IGNTKVISNEGIIDIKDVYPYKHTLYQNKSFEVVSNNIQYTKKKTIKIVTSKGYEIEG
                     TPNHKISKEGIFSRLDELKIKDKVDISFFMYPSNIPYVKEYVPFFLTKKLDSIDHSKL
                     DNNMISTITLNEEWGYFLGLFLGNGNISQSNSVNISCNSEEKDIIERLINFGNKIGVN
                     TFVRKSDKRNKSSICVHYSSRNLMWLLSNKFGFDGGKFKKNLNVPHQIFISPKSVVKS
                     FIQGLFDADGSVTTSSCYFTTKNKKLAQQIQFLLLGFNIQSKISSHWNKIYKRMYYSL
                     FLNRQASEIFYKEIGFINQKKNEKLKNVITKPYSNKYKKWEMNDTIVDIQENINDVYD
                     IEIPNNHYYVANGFISHNSNIKMLEYVAIGVPAVYSKIEPYKHACLQASSDDEIIGHI
                     EALVNDVDLRQRVWKQDYDTVKGQLWWEDNNFYNVRHYINANLKLFGKKVPF"
     gene            3463..4137
                     /locus_tag="P5293_05845"
     CDS             3463..4137
                     /locus_tag="P5293_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49459.1"
                     /translation="MTVFDVYKAFRKAQAEYYSRPYQLPKDFDYYLNTRMSKQNKAYL
                     EKAAMYFSTKWRNIDINKFMACGFELFGKNFTYVRFFNVKLLKFYIEKDKNKKRELRG
                     SKREIIASTKFVRRFLDQFKEKFDDILPITFYCRLKEGHQSIPVKHYIEGHIDKYFLV
                     WLINKRFISLTDDDRAQIPYITENYRQYVDIINNFQSFMERVFVVLNRRYENGKKDKQ
                     EGLTND"
     gene            4097..4282
                     /locus_tag="P5293_05850"
     CDS             4097..4282
                     /locus_tag="P5293_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49460.1"
                     /translation="MVKKINRKDLLTTKPPTGSMLYDKEFPEDIKKKYKEENLNEDKE
                     TSIKKRNSLHRQTKVIK"
     gene            4300..4449
                     /locus_tag="P5293_05855"
     CDS             4300..4449
                     /locus_tag="P5293_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49461.1"
                     /translation="MDKTYRTNRQILEDIERKIKQLIEKIEKLEQKMKNGGIYVLKMD
                     KERFI"
     gene            4415..5389
                     /locus_tag="P5293_05860"
     CDS             4415..5389
                     /locus_tag="P5293_05860"
                     /inference="COORDINATES: protein motif:HMM:NF020383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49462.1"
                     /translation="MCSKWIKKDLFDQFVEEKQQEETTKKSAPRRSETVWQTPSMGTQ
                     DKAKVYEIRFLPDPKGKFYKRFYYHGFKSGDRWYFPLCEKTYDFYNWCPFCAAASKLY
                     TGTAADKQQASVIKRKEKFVGNAFIVDDPRDAEREDKVVGTVKLYEFPSEVESKLKGT
                     ITDMKHGLGPAVFDPGDEGYNFIIKVKATKPTREGKQWPVYADSEFARKPSALAKTDR
                     EIQNILNQTFDIDEYIASQKMLDEDVIALLKNEMLWESVKEEWSRYKGKVANGDVRKE
                     VREEPKDSPFGAVKNSGTVVTSKKNVEEDVGEYEEPPDQDLLKELDEL"
     gene            5614..5892
                     /locus_tag="P5293_05865"
     CDS             5614..5892
                     /locus_tag="P5293_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49463.1"
                     /translation="MKKNLATIIMTLFVILITGTFTYAESVMVVNADNFPYFHLGTLI
                     VGGLIITSLQHKYNKIRLRESVGSFALYAILIALFTNPVIEMIRSLVG"
     gene            6229..6591
                     /locus_tag="P5293_05870"
     CDS             6229..6591
                     /locus_tag="P5293_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49464.1"
                     /translation="MSKRDEYLAKAMGICWHEPMTIVIEDQMFYICKHCGMMPMLRPD
                     KFIKPSFSEWIWFEFLLQWTKKNPYFMEMFMSEYNPWQQPVTPDLYADMVYAFLQQKE
                     MEEKENFFERIRMPNEKN"
     gene            6578..6889
                     /locus_tag="P5293_05875"
     CDS             6578..6889
                     /locus_tag="P5293_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49465.1"
                     /translation="MKKIEIVSPKEIHQLAKEKGWWDEHRPVPELLCLLHSEISEALE
                     AYRNDLPPTEEGGLFSELADSIIRIFDMCGAYNIDIAKLVEEKHKRNMLRPYRHGNKK
                     C"
     gene            7054..7449
                     /locus_tag="P5293_05880"
     CDS             7054..7449
                     /locus_tag="P5293_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49466.1"
                     /translation="MKETIVFCVPGKMFSYRFMRSWTNTIIECYKQEMPFIVQWAIGA
                     NVYQVRNQCLGGRVELGPNQKPFQGKFKYDFIMWIDSDQEWRVKDFNSLLQIMREKPE
                     IKALTGVYPRVDEDTSGISTICKITRYKS"
BASE COUNT         2808 a         1119 c         1453 g         2108 t
ORIGIN      
        1 ggtataataa ttgaaaatct tatcaggagg ttatttttat ggatagagat gatgtatgga
       61 atggcgccga agagcgtagt gcagagttca ccagtgagac ttgtgctctt gtaaacaatg
      121 catcaattcc acttactccc ggctcacagt tcaaagctac cgttctgcaa cttgcaaaag
      181 atgctggttt tggtaaattc cgaattgtgc taaatggcag tgaaatcaaa ccttcacagg
      241 cccccgaagt tattaatgag ggcgacaaga tccaaatcac cccatacgat gtcgctggtt
      301 tctaattgag aagggggtgg gtgcatagca actgccccca ctcaacaata aaaagaaagg
      361 tgtacagaaa taaatgacca cttatactgt taaaaaagaa gcttctgaac atcttctcaa
      421 tgaaatgtca aatgtattcg ccacgattga ttctatcaat atcaatgaca ttgaattaaa
      481 gggtttatca ataagaaccg gagataaact tttgaagctc aaagttgttg aagttgagag
      541 tctcgatcca attgaggaac taaaaagaga atacaaaaat atcctgaacc agaaacttgc
      601 agccataaaa gatacaatcc aaaagaaagt atcagatatg gtatcgtttg ttcaggtata
      661 tagagaagaa tttgaaagaa aagaaataga gctaaaaaag aaacttgagt cacaacaaat
      721 tatgcctcca atttcttttc agcaagcgga gcgcggattg tcagtatcgc ctggccagcg
      781 taatggagaa attcattggt ttgtaagaag tgtttattgg ccaaaatttg tcgatcaaaa
      841 accaatagaa acaaaatttc aaaagaagct tatcacacca attgttatat tgataacaac
      901 agaaggcgac catgtgaaaa tggtttcaac acgaacccca atcgggctca gatatttttc
      961 acactatcac caagcattgc cagattgttg gggtaaatgg aaatggaaac caaaatggga
     1021 atcaccggag gatattctgg caatagccaa agaagtcgag ggagtgctag aaaatataaa
     1081 cactgcctca atagccaata gatcaccagt aggtttaccc acactaaata tactaagtaa
     1141 acatatagtg ggtagaaaag aagatttgaa aaaaagagat gtaaatacga ctaatatgag
     1201 agccggtgtt tctacatcaa ctaacatagt aatagaggat agttggtcaa cataataaat
     1261 gaatttacag gttatacaaa aacctgcctc gattaacata ttgagttatg tcggagatac
     1321 acaggggtgt ggaacgataa gagttatcta tccacacctc ctgcttcctc acttacgata
     1381 tcatggatat aaatttgtgg ggttctactc tgcatatttc ataagacagg cagactatta
     1441 cagaaatttc agttttgtta tctttcaaag ggcagcaacc aaatcccatc ttgcccttat
     1501 atctaatttt atcaatcaag taacaaagac ctataaaaca cccataatct acgagattga
     1561 tgaccttctt atcaatattc cggagtggaa ttatgcgtca gattactaca aagaaaacga
     1621 aggccatata gaagagataa tgagaaaggt aaatggtatt accacatcta ccgaaaaact
     1681 taaggaagta tattccaaat ataattctaa tatagaagta attccaaatc atctacctaa
     1741 attcgtgtgg ggagaaatca ggcctaagca cgaaaactat ccaagaacgg aaaaagaaag
     1801 accaagaata ctctgggccg gatctgaaaa ccactttgct gtcaagccgg ataagacggg
     1861 tggtgatttt ggtgatgaat tactgaaata tattcgtggc acagttgaaa aatatcaatg
     1921 ggtcatttgt ggagggatac caaaagaact caaagacctg aaaggaaaaa tcgaatatca
     1981 cggttggagg aatatatttg aatatccatc ttttctcaaa agtcttgatg tggatatagc
     2041 cgttgccccg ttgatgcagg acatctttaa tgaatgtaag tgtttaattg gtaatacaaa
     2101 agtcattagt aatgagggaa taatagatat aaaagatgtt tatccctaca aacatacttt
     2161 atatcagaat aaatcatttg aagtggtatc taataatata caatacacaa aaaagaagac
     2221 tataaaaata gtaacatcaa aaggatatga aatagaagga acaccaaatc acaagatatc
     2281 caaagaagga atttttagta gattagatga gttaaagata aaagataaag ttgatatatc
     2341 attttttatg tatccatcta acattccata tgtaaaagag tatgttcctt ttttcctaac
     2401 taaaaaatta gatagtatag accattctaa attagataat aatatgatat caaccataac
     2461 tttaaatgaa gaatggggtt attttttggg gttattctta ggtaatggaa atatttcaca
     2521 gtcaaattct gttaatatat cttgtaatag tgaagaaaaa gatataattg aaagattgat
     2581 aaattttgga aataaaatag gtgtaaacac atttgtgaga aaatcagata aaagaaataa
     2641 atcaagtatt tgtgttcatt atagtagtcg taatttaatg tggttactta gtaataaatt
     2701 tggttttgac ggtggaaaat ttaagaaaaa tttaaatgtt ccacaccaaa tatttatttc
     2761 tccgaaaagt gttgtaaaaa gttttattca gggattgttt gatgcagatg gttctgtaac
     2821 aacctcctct tgttatttta caactaagaa taaaaaattg gctcaacaga tacaattctt
     2881 attattaggt tttaacatcc aatcaaaaat tagttcacat tggaataaaa tttataagag
     2941 gatgtattat tctcttttct taaacaggca agcatcggag atattttata aggaaattgg
     3001 ttttataaat caaaagaaaa atgaaaaatt aaaaaatgtt attaccaaac catattctaa
     3061 caagtataaa aaatgggaaa tgaatgacac aatagttgat atacaggaaa acataaatga
     3121 tgtatatgat attgaaatcc ccaataatca ttattatgtt gcaaacggtt ttatatcaca
     3181 taacagcaat ataaaaatgc ttgagtatgt tgcaataggt gttccggctg tttatagtaa
     3241 aatagaacca tataaacatg catgtttaca ggcatcgtca gacgatgaaa taataggaca
     3301 tattgaggca ttagtcaatg atgttgattt gagacaaagg gtttggaaac aagattatga
     3361 tacagtaaaa ggacagcttt ggtgggaaga taataatttt tataatgtta gacattatat
     3421 caacgcgaat ttaaaactat ttggaaagaa ggttccgttc taatgacagt atttgatgtt
     3481 tataaagcat ttagaaaggc acaggctgaa tattactcaa ggccatatca attaccaaag
     3541 gactttgatt attatttgaa tacgagaatg tccaaacaaa acaaggccta tctggaaaaa
     3601 gccgccatgt atttttctac aaaatggaga aatatagata ttaataaatt tatggcttgt
     3661 ggcttcgaat tgtttggtaa gaactttacc tatgtaagat ttttcaatgt caagcttttg
     3721 aaattttata ttgaaaagga taaaaataaa aaaagggagc taagaggtag taaaagagaa
     3781 attattgctt cgacaaaatt tgtcaggaga tttctcgatc aattcaagga aaaattcgat
     3841 gatattttac cgatcacatt ctattgtaga ttaaaggaag gacaccagag cataccagta
     3901 aaacattata tcgaaggaca tattgacaaa tattttttgg tatggctgat aaacaagaga
     3961 tttatctctt tgacagatga tgacagagca caaattccat atattacaga aaattacaga
     4021 caatatgtgg acatcattaa taatttccaa tcatttatgg aaagagtatt tgtagttttg
     4081 aacaggagat atgaaaatgg taaaaaagat aaacaggaag gacttactaa cgactaaacc
     4141 accaaccggt tcaatgttgt atgataagga gtttcctgaa gatataaaga aaaaatataa
     4201 ggaagagaat ctgaacgaag ataaggaaac ctctatcaaa aagaggaatt ctttacatcg
     4261 gcagacaaag gtgataaaat aactatatga ggtcaatgaa tggataaaac atatagaaca
     4321 aatagacaaa tacttgagga tatagaaagg aagataaaac aattaataga aaaaatcgaa
     4381 aaattagaac agaaaatgaa aaatggaggt atatatgtgc tcaaaatgga taaagaaaga
     4441 tttatttgac caattcgtag aagaaaaaca gcaagaagag acaacaaaaa aatctgcgcc
     4501 gcgccggtct gaaacagtat ggcagacccc ctcaatggga acacaggaca aggccaaggt
     4561 atatgaaatc aggtttcttc cagatccaaa aggaaagttt tacaagaggt tctactatca
     4621 tggatttaag tctggtgaca gatggtattt ccctctctgt gaaaaaacct atgatttcta
     4681 taactggtgc cccttctgtg cggcagccag taaattatat actggaacgg ctgctgataa
     4741 acagcaggca tccgtaataa agagaaaaga gaaattcgtc ggtaatgctt tcattgtgga
     4801 tgacccaagg gatgcagaaa gagaagataa ggttgtagga accgtgaaat tatacgagtt
     4861 tccttcagaa gtcgaatcaa aattgaaggg aaccatcaca gatatgaaac acggtctcgg
     4921 cccggcagta tttgacccgg gcgacgaagg atataatttc atcatcaagg tcaaggcaac
     4981 aaagcccaca agagaaggta aacaatggcc ggtatatgca gattcagaat ttgcaagaaa
     5041 accaagcgcc ttggcaaaaa ccgatagaga aattcaaaat attctcaatc aaacattcga
     5101 tattgatgaa tatattgcat cacaaaaaat gttagacgaa gatgttatcg ccctattgaa
     5161 aaatgaaatg ctatgggagt ccgtaaaaga agaatggtca agatataaag gcaaggtagc
     5221 aaatggtgat gtgagaaaag aagtgcggga agaacctaaa gattcgccct tcggagccgt
     5281 gaagaatagt ggcacagttg taacatccaa gaaaaatgtt gaagaggatg ttggtgaata
     5341 tgaggaacca cctgatcaag atttattaaa agagctggat gaactataat tccatcattt
     5401 ccattccgca agaaggggag ccgaaaggtt cccctttttt attgaacatt tcgttcaaca
     5461 ttgaacatta tgttttatcg tttcgttcaa tttaccccca aactttaaga taaccctttg
     5521 aaattattca actatcaatg ttggtatgat tcttgatacc ctatagggta gggataaaac
     5581 ccaaatactt cttttagaaa gtgaggaaat acaatgaaaa agaatttagc aacaattatt
     5641 atgacactct tcgtaatcct tattaccgga acattcacct atgcagaatc agttatggtg
     5701 gtaaatgctg ataacttccc ctatttccac cttggaacct taattgtggg cggtctgatt
     5761 attacttctc tccagcataa gtacaataag attcgtctga gagagtctgt cggttctttc
     5821 gctctttacg ccatcctgat tgctctgttc actaatccgg taatcgagat gattagaagc
     5881 cttgttggtt aatcctttat acacggggcg gtgatgagcc gccccatcaa aggagcaatt
     5941 atggtacatc aagtattatt tttaagtgcg tggagttttg caacagttat ttcatctttc
     6001 ctgtttctgt atatggggat atggatagat aagacccttc aaatggagcc caccttcact
     6061 attggcttgt ttgttcttgc tatcttttta tgcataggaa agatgtataa agaagcctgg
     6121 gaaaaacgaa atagataatc aagtctaatt tacaattaaa cgaaaatatg gtataataat
     6181 tcataaatat ggatatggaa atatctgtat ttaggaatta ttttgcttat gtcaaaaagg
     6241 gatgagtatc ttgcgaaagc aatgggtata tgctggcatg aaccaatgac aattgtaatt
     6301 gaagaccaga tgttttatat ctgcaaacat tgtggtatga tgccaatgct cagaccggat
     6361 aaatttatca agcccagctt ttcagaatgg atatggtttg aatttcttct tcaatggaca
     6421 aagaaaaatc catattttat ggaaatgttt atgtctgaat ataatccatg gcagcagcca
     6481 gtaactcccg atttatatgc cgatatggta tatgcctttc ttcagcaaaa ggaaatggaa
     6541 gagaaggaaa atttttttga aaggataaga atgcccaatg aaaaaaattg aaattgtgag
     6601 tccaaaggaa atacatcaac tggcaaaaga aaagggatgg tgggatgaac atcgcccagt
     6661 tccggaatta ttgtgcttgt tacatagtga aatttctgag gcacttgaag cctataggaa
     6721 cgacttgccc ccaactgaag aggggggtct tttttctgaa cttgcggaca gtataattcg
     6781 tatttttgat atgtgtggag cctataatat agatatagca aagttagtag aagaaaaaca
     6841 caaaagaaat atgttaaggc cgtatagaca tggaaacaag aaatgttaat atataaagta
     6901 accaatataa ttaatgataa aatatacata ggtcaaacag taaaaaatct tgatataaga
     6961 aagtcaagtc atctcctcga tgtgaaaaga ttatccatta ttataaatat aatatgacca
     7021 gaccgtatag acacggaaat aaggtatgtt aatatgaaag agacaattgt gttttgtgtg
     7081 ccaggaaaaa tgttcagtta tcgttttatg agaagctgga caaatactat tatagaatgt
     7141 tataaacagg aaatgccctt tattgttcaa tgggcaatcg gagcaaatgt ttatcaggta
     7201 aggaaccaat gtcttggtgg aagagtagaa cttgggccga accagaaacc ctttcagggt
     7261 aaattcaaat atgattttat aatgtggatt gattcagacc aagagtggag agtaaaagat
     7321 ttcaattccc tcttacagat tatgagggaa aaacccgaaa taaaagcatt gacgggtgtt
     7381 tatccaaggg ttgatgaaga tacttctggc atatcaacaa tatgtaaaat tacaaggtat
     7441 aaaagttgaa tatgtgaatc cacaatatac aagtcaaaga tgtccgtg
//
LOCUS       DAOTQL010000084         7452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8354_length_7452_cov_4.819116, whole genome shotgun sequence.
ACCESSION   DAOTQL010000084 DAOTQL010000000
VERSION     DAOTQL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8354_length_7452_cov_4.819116"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3457
                     /locus_tag="P5293_05885"
     CDS             <1..3457
                     /locus_tag="P5293_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR49467.1"
                     /translation="GVSTDKGFVLQDGKITISVTNKPIKEILDMIEKEGKCRFMYDDN
                     LEYMNKSVSINVKDVPISVFLEELFKNTDLVTQSMENNLIVITTRAIAQSTGILTNDK
                     KSENSDSQQLALTGIVKDLKTNEPIPFVSVIIKEDKSKGTFTDENGKFTIYPSTLQNT
                     LQLSIIGYKSIEVPINGRRTVDILMEPELTALDAVMIVAYGTAKRSTYTGSAATVSED
                     RLNARPVTAVSQALAGTTAGLQISTSNGQPGSVPTIRVRGLGSFNASNEPLIVLDGMP
                     YDNSLTSINPNDIESVTILKDASSAALYGARAANGVLLITTKKGKIGKVKVDAKYNLG
                     LTTRQTADYKTVGTRDYMQLYWESTRNSLMYSGATREEANARAGSAFLAGLSYNPYNM
                     DADQLFAPSTGKINPGAKLLWPDDLDWRGAIEQLGIRHDAVFSISGANDKTDYYTSIG
                     YLTEQGYIIGSELTRYSAKANINSQVNKWLKVGVNLNSSITESQGNQNESSGNNSNPF
                     RFLRYVGNIFPIHLHNPVTGEYIYDQQGNKLYDFGLGYTTEDGITVPKRDFVAGNNPA
                     IELQNIYDGYRRNTINAKTYAELTFLKDFKFSVNVGVGTNAYRGWNGTYVYKEKGNAG
                     TSTKSSSETTTWTINQLLTYSKEFGKHHFDAMLGHESYDYEYNYLSSSMKGQIMIGSN
                     FEFKNFTEVNAIPNSYTNKYRVEGFLSRINYDYDNTYFGSVSFRRDGTSRFYKDARWG
                     NFWSAGVGWRVDKEQFMRNLKFIDLLKIRTSYGVVGNDDLDSYYPWRATYYPYPNGTE
                     PGYLQDALGNKELSWEVSHNFDVAAEFSLFDSSFSGTFEFFNRESSNLLFEVPQPLSS
                     GVETQSINAGTMYNRGIEATFDYDLIKKKNFRMNVNLNATFLENKIKDLPLDPYVSSV
                     YKIEEGHSRYEFWLRQWKGVNPATGYNLFEADLENESYVWGEGELIDVNGVQCTEVIE
                     HAKYDWSGNATPLITGGFGSAINYKNWSFSFNFYYQLGGKYYDSTYESLMTVGTASLS
                     YSKLHVDLLNRWRESGDITKVAKLSSGTDSKNIGAGTSTRWLVSSNMLELTNVNLSYS
                     FPKKVLDKLSIDGIKVYFSADNSLLITARRGMFPRRTIFSGYDGNADIYLPSRTFSFG
                     INMNF"
     gene            3469..5094
                     /locus_tag="P5293_05890"
     CDS             3469..5094
                     /locus_tag="P5293_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR49468.1"
                     /translation="MNKRIKFIILLSGISLMSFSCSSDFLETASTSSVDQAQMFETTS
                     SAMMAVNGIHRLMYLPGSSAAQCGYGTFMLWMDMMGEDLVYTKGNAQWQSQAKWTLHR
                     NTTSSHNKFLYKFFYSIISNANMILDNIDNANGLQEEKDYIKGQALTYRAFAHFSLVQ
                     LWGERYYPGQNNTQDGIVIKLDNSLEPLPRSSVEDVYAVINDDLDNAIELLEKVNIKR
                     NSITHINIHVARAIKARVLLTQGKWMEAAQMAQLVVENSGASLQADTYAFKQGRMCDA
                     TNKEWIWGKIGQPTLETGTLTNFFSFISNTNVSYNKNTPRAIYNLLYNRISSTDVRKQ
                     VWLPDAPLMDRKLIAYPPAGNIFKWMSQKYIVDYPDNTSSLYLGNVYTADLPYIRLPE
                     MILIMAEGYARAGSDQLAAQALYPLAAVRDPQYILSTNTGEALIDEIMFQRRIELWGE
                     GFRFLDLKRLNMNLDRGPAPRPGYNQGGSANGWKSGQNPKNLDPLASNYNMYDDQGLG
                     EENRFKAANSIEWQFVFPQSEIDYNPLCKQNPI"
     gene            5192..>7452
                     /locus_tag="P5293_05895"
     CDS             5192..>7452
                     /locus_tag="P5293_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRR49469.1"
                     /translation="MAQTETVLANGINCYLPEGKYYFNSDIPTPSSVLGFNVGDQYAD
                     WNDILKYMKALDQVSDRVSIREYGETYQHRRFIQVVITSVENQRNLETLQKEHLQSIQ
                     KAWEGNSKGESGDLSKTPVFVNLMNSIHGNEASGANASLILAYFFTAVENPDILKLLD
                     NTIIIITPGLNPDGINRFASWVNSSRSFCDIADLNSREFSEPWPSSRTNHYWADCNRD
                     WLSVQHPEGLNAVKMYRAWLPNVVVDQHEQGGDGKGFYFSPGHPLRTHPDISDENQKL
                     TSEITSYIASGFDKAGVLYYSKDGYDDFFVGKGATYGDINGSIAILVEQLASRGFMRP
                     TSMGNLSFPATVKNQVFAGYYSIIAAYSLKDKLIEYQRSFFANLSRSASKDKTQGYIF
                     TTDGLKGIAHYFLTNMQSHNIEVYRLKKDCTADGRTFKANDSYVIPVNQINYNIIKTM
                     WEEMKNYRDSTFYDISTWTFPYAYNLRYCTVENISGLIGNKVDSVQIIPGKIKGGKSI
                     YAYIFENSEYFTPKLIFELLNRDLYLRISDRQFTSMDAGFKPGSIVIPVAGQPLDGEE
                     LYSLLERLSLENGVDIISMKSGLMKDYDLGSPVFKVLRKPEIAMIVGKGMGIPESGEI
                     WHMLDQRFKIPVTLIDHTLLPNTDLRKYNVIIFADGMLSNSSIGSFAENLKRWIRDGG
                     VLIATGNSYTITNEAKLTGIKTLPAVKKDSFDGVILKCIFNKHNPLGWGCDNTSIPIM
                     RTVPVVINKTELK"
BASE COUNT         2496 a         1242 c         1513 g         2201 t
ORIGIN      
        1 cggagtgtca acggataagg gttttgttct acaggatgga aaaataacta tatcagttac
       61 aaacaaaccc attaaggaga tacttgatat gattgagaaa gaggggaaat gtagattcat
      121 gtatgatgat aatcttgaat acatgaataa gagtgtatca atcaatgtaa aggatgttcc
      181 catttctgtt tttttagaag agctgtttaa aaatactgac ttggtaacac agagtatgga
      241 aaataatttg atagtaatta ctaccagagc catagctcaa agtaccggta ttttaacaaa
      301 cgataagaaa agcgaaaatt cagattcaca acagctcgca ctaacgggaa ttgtaaaaga
      361 tttaaaaaca aatgaaccaa tcccttttgt ttctgtaatt attaaagaag ataaaagcaa
      421 gggtactttt acagatgaaa acggaaaatt tactatttat ccaagcactc ttcagaacac
      481 gcttcaactc agcattattg gatataaaag cattgaggtt ccaataaatg gtagaagaac
      541 tgttgacata ttaatggaac cagaattaac tgctttagat gctgttatga tagttgctta
      601 tggcacagct aagagaagca cttatacagg gtctgcagca accgttagtg aggatcgttt
      661 aaatgcgaga cctgtaactg cagtctcaca ggcacttgcc ggaaccactg ctggacttca
      721 gatcagtaca tcaaatggtc agcctggttc tgtaccaaca atcagagtta gaggacttgg
      781 ctcatttaat gcaagtaatg aacccttgat agttttggat gggatgccgt atgataattc
      841 tttgacaagt atcaatccta acgatattga aagtgtaaca attctcaaag atgcatcttc
      901 agcagcctta tatggagcga gggctgcaaa tggagtcttg ctgattacta caaaaaaagg
      961 caaaattgga aaagttaagg tagatgcaaa atataattta ggattaacaa cacggcagac
     1021 agccgattac aagaccgttg gcacaagaga ctatatgcaa ttatattggg agagtacaag
     1081 aaatagttta atgtacagcg gagcaacacg tgaagaagca aatgcacgtg caggttcagc
     1141 ttttttggct ggtttgtcat acaatccgta taatatggat gcagaccaac ttttcgcccc
     1201 atcaacaggg aagataaatc ctggagcaaa attgttatgg cctgatgatc tcgactggcg
     1261 aggtgccatt gagcagttag gtattcgtca tgatgcggtt ttttccatat caggggcaaa
     1321 tgataagacg gattactata cctctattgg ttatttaaca gagcagggtt atataattgg
     1381 ttcggaatta acaagatatt ctgcaaaagc aaatataaac tctcaagtaa ataaatggtt
     1441 aaaagtaggt gttaatctaa acagttcaat tactgaatca caaggcaacc agaatgaatc
     1501 cagtggcaat aattcaaatc ctttccgttt tcttagatat gttggaaaca ttttccctat
     1561 tcatctccac aatccagtaa caggtgaata catttatgat cagcagggga ataaactata
     1621 tgattttgga ttgggatata ctacagaaga cggaattact gtaccaaaga gagatttcgt
     1681 ggctggcaac aaccctgcca tagaattaca gaatatttat gacggttata gaagaaatac
     1741 catcaatgca aaaacatatg ccgagttgac atttctcaag gatttcaagt tttctgtaaa
     1801 tgttggagtt ggcacaaacg catatagagg atggaatgga acttatgtct acaaagaaaa
     1861 aggcaatgca ggtacatcta caaagtcatc ttctgaaaca actacatgga ctataaatca
     1921 gttacttaca tattcaaaag aatttggcaa gcatcatttt gatgcaatgt taggacatga
     1981 gagctatgat tatgagtata attatctttc ttcctcaatg aaggggcaaa ttatgattgg
     2041 atcaaatttt gagtttaaga attttactga ggttaatgct attcctaatt catacacaaa
     2101 caaatacaga gtagaaggtt ttttgtcgag aatcaattat gattatgaca atacttattt
     2161 tggttcagta tcttttagaa gagatggcac atcccgcttt tacaaagatg cacgttgggg
     2221 caatttctgg tctgcaggtg ttggatggag ggttgacaaa gaacaattca tgaggaattt
     2281 aaaatttatt gatttgctca aaataaggac ttcatatgga gttgtaggaa atgatgatct
     2341 tgacagttat tatccttgga gggcaactta ttatccatat cccaatggca cagaaccagg
     2401 atatcttcag gacgcacttg gtaataagga gctttcatgg gaggtctctc acaattttga
     2461 tgtggcggca gaatttagtc tttttgattc ttcttttagt ggaacctttg aatttttcaa
     2521 tagagaatcc agcaacctcc tttttgaagt tccacagcca ctttcttccg gagtagagac
     2581 tcagagcatt aatgccggaa ccatgtacaa caggggtatt gaagcaactt ttgattatga
     2641 tcttatcaaa aagaaaaatt tcagaatgaa tgtaaatctg aatgcaactt ttcttgaaaa
     2701 taaaataaaa gacttacctc ttgatcccta tgttagttca gtctataaga ttgaagaagg
     2761 acattcccga tatgaatttt ggcttagaca atggaaaggg gtcaaccctg caaccgggta
     2821 caatcttttt gaagcagatc ttgaaaatga atcatatgtt tggggagagg gagagttaat
     2881 tgatgtaaac ggagtacagt gtaccgaagt gatagaacat gccaaatatg attggagtgg
     2941 taatgcaaca ccattaataa caggaggatt tggatctgct atcaattata agaactggtc
     3001 tttttcattt aatttttatt atcaactggg aggaaaatac tatgacagca cctatgaatc
     3061 acttatgact gtcggcacag cctcgctatc atattccaaa ctgcatgtgg atttattgaa
     3121 ccgttggaga gagagtggtg atattactaa agttgcaaaa ctttcgagtg ggactgattc
     3181 aaaaaatatt ggagccggaa cttcaactcg ttggcttgtc tcttcaaaca tgcttgaact
     3241 tacaaatgtg aatctatctt attcatttcc taaaaaagtt ttagataaat tgagcattga
     3301 tggcattaag gtctattttt ctgcagataa ctcgttatta ataacagcca gaaggggaat
     3361 gttcccaagg agaacaatct tctcagggta tgacgggaat gctgatatat atctaccatc
     3421 aagaacattt tcatttggaa ttaacatgaa tttctaaatt ttgcgattat gaacaaacga
     3481 attaaattta taatattatt atcaggtatt tcacttatga gtttttcctg tagtagtgac
     3541 ttcttagaaa cagcatctac atcttcagta gatcaggctc aaatgtttga aaccacttca
     3601 tctgccatga tggctgttaa cggcattcac agacttatgt acttacccgg ttcaagcgca
     3661 gctcagtgtg gatatggtac atttatgtta tggatggata tgatgggaga ggatttagta
     3721 tatacaaaag gaaatgcaca atggcaaagt caagccaaat ggactttgca taggaatacc
     3781 acttcttctc acaataaatt tttgtataaa ttcttttatt caatcatttc taatgcaaac
     3841 atgatattag acaatataga taatgccaat ggtctgcagg aagaaaagga ttatattaag
     3901 ggtcaagcat tgacctacag ggcttttgct catttctcgc ttgttcagtt atggggagaa
     3961 aggtattatc ccggtcaaaa taatactcaa gatggtatag taattaaact cgataatagc
     4021 ctcgaaccat taccacgttc aagtgtagaa gacgtatatg ctgtaatcaa cgatgacttg
     4081 gataatgcaa ttgaacttct ggaaaaggtt aatataaaac gcaattccat aacccatatt
     4141 aatattcatg ttgcgagggc tattaaggca agagtattac ttacacaagg caagtggatg
     4201 gaagccgcac aaatggcaca actcgtcgta gagaattcag gagctagttt gcaggcagat
     4261 acttatgctt tcaagcaggg tcgcatgtgt gatgcaacaa ataaagagtg gatttggggc
     4321 aagattggtc agccgacatt agagacaggg acgttgacta atttcttcag ttttatatca
     4381 aacaccaatg tttcatacaa caaaaatacg ccgagagcca tttacaattt gctttataat
     4441 agaatcagtt caactgatgt gcgtaaacaa gtatggctgc cggatgcacc tttgatggac
     4501 agaaaactca tagcgtatcc acctgcagga aatattttta aatggatgag ccagaaatat
     4561 atagttgatt atcctgataa tacctcatca ctttatttag gaaacgttta tacagcagat
     4621 ctcccatata taagacttcc tgaaatgata cttattatgg ctgaaggtta tgcaagagct
     4681 ggatcagacc aattggccgc acaggcacta tatccgcttg ctgcagtaag agatcctcag
     4741 tacatcttat ctacaaatac cggagaggcg ttaatagatg agattatgtt tcaacgaaga
     4801 attgagcttt ggggcgaagg atttagattt cttgatttaa aacgtttgaa catgaatctt
     4861 gacagaggtc ctgctccaag gccaggatat aatcaaggtg gcagtgctaa cggctggaaa
     4921 tccggtcaaa atccaaagaa tcttgaccca cttgcatcaa actacaatat gtacgacgat
     4981 caaggacttg gagaggaaaa tagatttaaa gctgccaaca gtatagaatg gcaatttgtt
     5041 tttcctcaat cggagattga ctataatcca ctttgcaagc aaaatcctat ataattaaca
     5101 tataatgacc tgtttaacaa attttaaaaa agacttattg attttttcag cactgttaat
     5161 tacagtgctg aattctatta ccacgatttc aatggcccaa acagagacgg ttttggcaaa
     5221 cgggataaat tgttatctgc ctgaagggaa atattatttt aatagcgata tccctacgcc
     5281 gtcgtctgtg ttgggattta atgtcggcga ccagtatgca gactggaatg atatattgaa
     5341 atatatgaaa gctcttgacc aagtttcaga cagagtatca ataagggaat acggagaaac
     5401 ctatcaacat agaagattta ttcaagttgt aataacttcc gtagagaatc agagaaattt
     5461 agagacattg cagaaggaac accttcaatc aattcaaaaa gcttgggagg gcaattctaa
     5521 aggagaatca ggggatctgt caaaaacacc agtatttgtc aatcttatga attccattca
     5581 tggcaatgaa gcttccggag caaatgcatc tcttattctt gcatattttt ttactgccgt
     5641 tgaaaatccg gatatattaa agcttcttga caatactata ataattatta ctcctggttt
     5701 aaatccagat ggtattaaca ggtttgcttc ttgggtaaac tcttcgagaa gtttctgtga
     5761 tatagcagat ctgaattcaa gagaattctc agaaccctgg cccagcagca gaacaaatca
     5821 ttactgggct gactgcaatc gtgactggct ttctgtacaa catccagaag gactcaatgc
     5881 tgtcaaaatg tacagagcat ggttgccaaa tgttgtggta gaccagcatg aacaaggcgg
     5941 agatggtaag gggttttact tcagtcccgg ccatcctttg cggactcacc ctgacatttc
     6001 tgatgaaaat caaaagctca cgtcggaaat aacatcctat attgcttccg gttttgataa
     6061 ggcaggagta ctttattact caaaggatgg ttacgacgat ttttttgtgg ggaaaggagc
     6121 aacttatgga gatattaacg gttcaatagc aattttggta gagcagcttg catccagagg
     6181 ctttatgaga ccaacctcta tggggaatct ttcatttcca gctactgtca aaaaccaggt
     6241 atttgcggga tattattcta ttattgctgc atacagttta aaggataaat tgatagaata
     6301 ccagagatca ttttttgcca atttatcacg atctgcctca aaagataaaa ctcagggata
     6361 tatttttacg acagatgggt tgaaaggcat cgcacattat tttttaacca atatgcaaag
     6421 ccacaatatt gaggtttaca gattaaaaaa agattgtact gccgatggaa gaacttttaa
     6481 ggccaacgac tcctatgtta tacctgtcaa tcagataaac tataatatta tcaagacaat
     6541 gtgggaggag atgaaaaatt accgtgacag tactttctat gatatatcaa catggacttt
     6601 tccttatgca tacaatttga gatattgtac tgtagaaaac atttcaggac tcattggaaa
     6661 caaggttgat tcggtacaga taatacccgg aaaaataaaa ggaggaaaga gtatttatgc
     6721 atatattttt gagaatagtg aatactttac accaaaattg atttttgagc ttttgaatcg
     6781 tgatttatat cttagaattt ctgatagaca atttacaagc atggatgccg gatttaagcc
     6841 tggttcaatt gtaatacctg tagcagggca accacttgat ggtgaggaac tgtattcatt
     6901 gctcgaacga ctttctttgg agaacggcgt tgatatcatc tcaatgaaaa gtggattgat
     6961 gaaagactat gatcttggca gtcctgtttt caaagttctt agaaagcctg aaattgcgat
     7021 gattgtgggt aaaggaatgg gcattcctga gtcaggagag atatggcata tgctggatca
     7081 aaggtttaaa attccggtaa ctctcattga tcacacatta ttacccaaca cagacttaag
     7141 aaaatataat gtaatcatct ttgctgatgg gatgttgagc aattcttcaa taggcagttt
     7201 tgctgaaaac ctcaaaagat ggattagaga cggaggtgtt ttaattgcta ccgggaattc
     7261 atatacaatt accaatgagg caaaacttac cggtatcaaa acattaccag cagttaaaaa
     7321 agatagtttc gacggagtta ttctcaaatg tatttttaat aaacacaatc cattgggatg
     7381 gggatgcgac aatacttcta tcccaattat gaggactgtg ccggttgtta taaataaaac
     7441 cgaacttaaa ag
//
LOCUS       DAOTQL010000085         7356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8484_length_7356_cov_4.500753, whole genome shotgun sequence.
ACCESSION   DAOTQL010000085 DAOTQL010000000
VERSION     DAOTQL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8484_length_7356_cov_4.500753"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..687)
                     /locus_tag="P5293_05900"
     CDS             complement(91..687)
                     /locus_tag="P5293_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRR49470.1"
                     /translation="MRYKIIILVTAFFCVTSDPLFSDGSEDSFYSELSRRSSEVISLN
                     SRFTQTKHVKLLNTKVVSQGKFYYKREGEIRFDYSTPKIMSIIMLKGKLRIETQSKAT
                     TYDFARQPSLAELAAIIEACVSGNIKNLPDKYKVVYTLLPDCHVLTITNLKKTPDNPY
                     TKIELHFDIDNYSLKELILYERSEDTTYYSFNNIVANG"
     gene            complement(684..1979)
                     /locus_tag="P5293_05905"
     CDS             complement(684..1979)
                     /locus_tag="P5293_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR49471.1"
                     /translation="MMRDSEISFKSKEEIKFFQEESLKQTISYIAENSPFYQRLFRQA
                     GVDPSSIRKTEDLSKLPTTSKKDVSEYNRDFLCVSKSLVMDYVTTSGTLGDPVTFMLT
                     ENDLDRLAYNDSLSFKGAGCKPGEVMQIMTTIDRRFMAGLAYFMGARKLGMGSIRVGN
                     GIPELQWDSIIREKPDSCIVVPSFLIKLAEYAEEHGIDYSDTSLRRAICIGEPLKNYD
                     LTTSTLTKKIIAKWPNLSLHSTYASTEMQSSFTECEYKCGCHHQPDLIIIELLDENEK
                     PVDQGEMGELTITTLGVEGMPLVRFKTGDVCYMIEEKCKCGRNSVRLSPIVGRKGQMI
                     KYKGTTIYPSALYDVLDNIPGVINYQVQVYTNTLGTDGILIKIGASDPSEAFEKDIKD
                     FFRARIRVAPEITFMPVELIAKSVFPQTSRKAVKFIDLR"
     gene            complement(1991..3574)
                     /locus_tag="P5293_05910"
     CDS             complement(1991..3574)
                     /locus_tag="P5293_05910"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HRR49472.1"
                     /translation="MKYLFKTIKYLAAFLLSLIALIFVGGMVLYFSADRSVPVDENFK
                     LRGEVISHEGGSTQYDSSFLRQARQGLWEIYLKGGAEERGAAIGQLCKSLMYEQEKAF
                     VDQIYEMIPSIGYVNFLKYLTVIYNRNLKRDIPDEFKKEIYASSLGCSHEFDFIGDAY
                     DRQLNYHAAHDIGHVMQEYMLVGCTSFAVWGSQSSDTTLIVARNFDFYAGDKFAENKL
                     ISFYFPDLGYKFASVGWAGMTGVLSGMNEKGLTVTINAAKSRPPLSSKTPISIITRQI
                     LQYASNIEEAYNIASSMKSFVSESILIGSASDGYAAIIEKSPQSTVLYREKGERIVST
                     NHFLSPENMSRLSKEDKLELEKGHSLYRYRRVEELLDSIGTMTPEKAAFILRDPFGLH
                     GKHIGWTNEMAINQFIAHHAVIFQPEKRLMWVSTSPWQVGEMVAYDLGDVFKKGSQGG
                     IDQNRSKVTSIPADSLLLNNVCPKVVEFRKLAGIINEAVKERKSIDSNVLQRFVEINP
                     DYDYTYSLIDKYNSSSKKD"
     gene            complement(3571..5034)
                     /locus_tag="P5293_05915"
     CDS             complement(3571..5034)
                     /locus_tag="P5293_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRR49473.1"
                     /translation="MGKYNAIIIGGGLGGLETALLLSKEGMSVCVLERNSKAGGLLQS
                     FSRSGKLIDSSVHYVGSMDKDEALYSYFKYLGILEDLDFVRLDNDCFDNIKTEDNSYS
                     FPMGLSNFEEYLLRKFPSESKVIPIYCRYLREVGKLSEPEHLKRGIFNLDYMDYYTYS
                     ASGVIRNYTKNPVLFDALWGTSLLYGGIEKVTPFYIHAITLYSNLKGAYRLRGGTSSV
                     VEALVSKIEENGGKVLTGQEVTKIVVEGSKVKGVITNNNDFYECDYVVSSTHPAETFN
                     LLDKNPLIKNSFIARILSEKNSYPLFCLYLVMKPGSFKYINRNFFIRRGNKRVNYVLL
                     SMQPPAVGSDFAEVVTIVTSSSFSQFEKWKDTRTGNRGEDYLEYKENLEEEILDFVAA
                     DFPDLRSSIECKFSASPLTFRDYTKTPEGSAYGIQKDCNKPFSTFIACKTKLPGLYLT
                     GQSNNVHGVLGATITSLLTCSDILGQDYLMNKIIRAI"
     gene            complement(5021..>7356)
                     /locus_tag="P5293_05920"
     CDS             complement(5021..>7356)
                     /locus_tag="P5293_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRR49474.1"
                     /translation="QCDSILDLMRREGIEIKKSSVASLVIPHSIQQERIDRWNQYWQE
                     KSSVVKHLISSESKKIGFSDEAFQAFYDLIDKKYEVFDYQSHKTDFPKLLSEWCELSD
                     SSSVFITQLFVDEEVKSNVYNKISSETQSVIVDRAYFAGLWATSVKDDFYFILTLSSL
                     LVFLTLLVSYGRIELALLAFLPMFVSWIIILGMMAICGIKFNIVNILLSTFIFGIGDD
                     FSIFVLDGLQGEYAKRNTILSSHKNAIFFSTFTVIVGMGVLIFAKHPALRSIATISTL
                     GMMAVWFVALTVQPVLFRHFITYPVRRSLPPYTVSGILLMFITFSIFVIGCILAAVYI
                     SIAAIIPVPVKYKKKVFHKMLSALSMLPVRISPTVKIFKENPYNEDFKKPAIIIANHQ
                     SFIDILMMLSLNPNIIMMTNRWVWNSPVFGHIVRYAGFIYYQDGIENHIEQVKKMTEN
                     GYSVVIFPEGTRSKDLKIHRFRKGAFYIAEKLSLDILPVIMYGNGHLVSKQQPFYVKH
                     GIAGYRILPRISCNLTSGTGKDYSTLCKETCAYMRKEYDKLRKRFDTPQNKYFYYSVI
                     HNYIYKGPVLEWYMRIKIKMENNYSYFHDLIPLNARLTDIGCGYGPLCFMLGLMSEDR
                     HILGIDYDKEKIDIARNCYLAGDRIRFEYADALIYEYPECDVFVMNDILHYLSVEDQY
                     RLLEKTVCRLSKEGFIIVRDGDNLSKNSHLVTKLSEWFSINLLGFNKASHPLCFINIL
                     IMENWAKRLGCSLEFVRNDKLTSNTIYLLRKLPDGQV"
BASE COUNT         2365 a         1488 c         1139 g         2364 t
ORIGIN      
        1 ccttgagata gtaatggagt aagcagactc ttgagtatcg caagtatctg ccctgtctga
       61 gtatagtaaa agacagctat ttggtatttt ttatccattt gcaacaatgt tattaaatga
      121 atagtatgta gtatcttctg atctctcata caaaatgagc tctttcaaag aataattatc
      181 tatatcaaaa tgaagctcga tttttgtata aggattgtct ggtgtttttt ttaagttagt
      241 aatcgtgagg acatgacaat ctggcaaaag agtatagact actttatact tgtcagggag
      301 attcttaata ttgccactta cacatgcctc gatgatagca gcaagttccg caagcgaagg
      361 ttgtcttgca aaatcatagg ttgtggcttt gctctgtgtc tctatccgca acttaccctt
      421 taacattata atactcatta tcttgggagt tgagtaatca aatctgattt ctccctctct
      481 tttgtaataa aatttacctt gagatacaac ttttgtattt aataatttaa catgttttgt
      541 ctgagtaaat cgtgagttaa gcgaaataac ctctgaagaa cgccttgaaa gctcagaata
      601 aaacgaatcc tcactgccat ccgaaaataa aggatctgaa gtgacacaaa aaaacgcagt
      661 taccaatata atgatcttgt acctcatcgt aagtctataa atttaacagc ttttctacta
      721 gtttgaggaa aaacagattt agcaattaat tcgactggca taaaagtaat ttccggagct
      781 actctgattc gagctctgaa aaaatcttta atatcttttt caaaagcttc acttggatca
      841 gaggctccta tcttgataag aattccgtca gttccaagtg tgtttgtgta aacctgcacc
      901 tgataattaa taactccggg gatattgtca agaacgtcat acaaagcact tggataaata
      961 gtggtcccct tatattttat catttggccc tttcgtccta ctatcggact taatctaacg
     1021 ctatttcttc cacacttaca tttttcttca atcatataac atacatcccc tgtcttaaat
     1081 cgtaccagcg gcattccttc cactcccagt gtagttatgg ttagttcacc catttctcct
     1141 tgatctacag gcttttcatt ttcatccaac aattcaataa ttataagatc tggctgatga
     1201 tggcagccac acttgtactc acactcagta aaagagctct gcatttcggt agaagcatat
     1261 gtagaatgaa gagaaagatt tggccatttt gcaattattt tttttgtgag tgtactcgtt
     1321 gttaagtcat agttcttaag aggttctcct atgcagattg ctcttcttaa agaagtatcg
     1381 ctataatcta tgccatgttc ttcggcatat tctgccaatt ttataaggaa agatggcaca
     1441 acaatacagg aatctggttt ctctcgaatt attgaatccc actgaagctc cggaatgccg
     1501 tttccaactc ttatagagcc catgccgagc tttcttgcgc ccataaaata tgcaagtcct
     1561 gccataaaac gacgatcaat ggttgtcata atctgcatta cttcccccgg tttacaccca
     1621 gcccctttga aagagagaga atcattatag gcaagacgat caaggtcatt ctctgtcaac
     1681 ataaaagtaa caggatcacc taaagtgcct gaagttgtga cataatccat caccaagctc
     1741 ttcgaaacac aaagaaaatc ccgattgtac tcagaaacgt cctttttaga agtagtaggc
     1801 aatttggata agtcctctgt ttttcttatt gaggaaggat ctactccggc ctgtctaaat
     1861 aacctttgat aaaatggaga gttttcagct atatagctaa ttgtctgctt taaagactct
     1921 tcttgaaaaa atttgatctc ttccttagat ttaaaagaga tctccgaatc cctcatcata
     1981 atcatgatac ttaatctttt ttacttgaac tgttgtattt atcaatcaat gaatatgtat
     2041 aatcataatc tggattaatc tcaacaaatc tctgaagaac attgctgtca atacttttcc
     2101 gctcttttac agcctcattt attattccag caagttttct aaactcaact acttttgggc
     2161 aaacattgtt caacaacaat gaatcagcag gtatactggt gacttttgat ctgttttggt
     2221 caatacctcc ctgactacct tttttgaaaa catctcccaa gtcatatgca accatctctc
     2281 caacctgcca aggagatgta cttacccaca tcaatcgttt ttctggttga aatataacgg
     2341 catgatgtgc aataaactga tttatagcca tttcatttgt ccatcctatg tgtttcccat
     2401 gcaaaccaaa aggatctctc agtataaatg cagctttctc aggtgtcatg gttccaattg
     2461 aatcgagaag ttcctcaacc cttctgtatc gatacaggga atgtcctttc tcaagttcca
     2521 atttatcctc cttacttaat ctactcatat tctccggaga aaggaagtga tttgtagaaa
     2581 caatcctttc acccttttct ctataaagca ctgttgactg tggagatttt tcaataattg
     2641 cagcatagcc gtcacttgct gaaccaatca gaattgactc tgaaacaaat gacttcattg
     2701 atgaagcgat attatatgcc tcttcaatat ttgaagcgta ctgaagaatc tgcctcgtga
     2761 taatagaaat cggcgttttt gaagagagcg gaggccttga tttggccgca tttattgtaa
     2821 ctgtcaaacc tttttcattc atcccggaaa gaacacccgt cattcctgcc catcctacag
     2881 atgcaaactt gtatcccaaa tcaggaaaat aaaaagagat taatttattt tctgcaaact
     2941 tgtcaccggc ataaaaatca aaattccttg caacaatcaa tgtagtatct gaagactgag
     3001 aaccccaaac agcaaatgaa gtacaaccaa ccagcatata ttcctgcatc acatgtccta
     3061 tatcatgtgc tgcatgatag tttaactgac gatcatatgc gtctccaata aaatcaaact
     3121 catggctaca tcccaaagaa cttgcatata tttctttttt aaactcatcc gggatgtctc
     3181 tcttaagatt tctattataa attactgtta ggtatttaag aaagttaaca tatcctatag
     3241 atggtatcat ttcatatatt tgatctacaa atgctttctc ctgttcatac attaaacttt
     3301 tgcacaactg cccaatagca gcacctctct cttcagcacc acccttaaga taaatttccc
     3361 ataacccttg tcttgcctgt ctcaaaaatg aagagtcata ttgagtgcta cctccttcat
     3421 gtgagataac ttctcctcgc aatttaaagt tctcatcaac aggaactgat cgatcggcag
     3481 aaaaatatag aaccataccg ccaacaaaaa ttaaagctat cagtgaaagc agaaaagctg
     3541 ctaaatattt tattgtttta aataaatatt tcatatagcc cttattattt tattcatcag
     3601 gtaatcttgc cccaatatgt cggaacatgt gagtaaagaa gtaatagttg ctccaagcac
     3661 accgtgaaca ttgttacttt gccctgtaag ataaagtcct ggcaatttag tcttacaggc
     3721 aataaatgtt gaaaatggtt tattgcaatc tttttgaata ccataggcac ttccctcagg
     3781 tgtttttgta taatctctga aagtcaaagg tgaagccgag aatttgcact cgatagaact
     3841 ccgcaaatca ggaaaatctg ctgccacaaa atcaaggatt tcttcttcca agttttcttt
     3901 atattccaaa taatcctctc ctctgtttcc tgtcctggta tctttccact tttcaaactg
     3961 agaaaaggat gaacttgtaa caatagtcac gacctctgca aaatcactcc ctacagcagg
     4021 aggctgcatt gataaaagta cataatttac ccttttattt cctcttctta tgaagaaatt
     4081 tctgtttatg tatttaaaag aacctggttt catcacaaga tacaaacaaa aaagcggata
     4141 tgagtttttc tctgaaagta ttctggcaat aaaagagttt ttaattagag gatttttgtc
     4201 aagtaaattg aaagtttcag caggatgagt agaagatacg acataatcac attcataaaa
     4261 atcgttatta ttcgtaataa ctcccttaac tttggatcct tcaactacaa ttttcgtgac
     4321 ctcctgcccg gttagaacct taccaccatt ttcttcaatt ttacttacca aagcttctac
     4381 cactgaagat gtaccacctc ttaaacgata agctcctttt aaattggaat acaaagtaat
     4441 ggcatgaata taaaacggag tcaccttttc tatcccgcca taaagcaatg acgtacccca
     4501 cagtgcgtca aaaagaaccg gattctttgt ataatttcta ataacaccag aagctgaata
     4561 tgtataataa tccatgtaat cgagattgaa aatgcccctt tttaagtgtt cgggttctga
     4621 cagtttacct acttcacgta aatatctgca ataaattggg atcactttac tttcagatgg
     4681 aaactttctt aaaagatact cttcaaaatt tgataagccc ataggaaaag aataactgtt
     4741 atcttcagtt ttaatgttat caaaacaatc attatcgagt cttacaaaat cgagatcttc
     4801 caaaatacct aagtacttaa aataactgta taaagcttca tctttgtcca tgcttccaac
     4861 ataatgcact gaagaatcaa taagtttccc ggatcttgaa aaggattgca aaagtccgcc
     4921 tgctttactg tttctttcaa gaacacaaac actcataccc tcttttgata ataaaagagc
     4981 agtttcaaga cctccaagac ctcctcctat aattattgca ttatacttgc ccatcaggta
     5041 attttcttaa caaatatata gtatttgatg tcagcttgtc attcctgaca aactcaagtg
     5101 aacatcccag tcttttggcc caattctcca ttatcaaaat gttaataaaa catagaggat
     5161 gcgatgcttt gttaaaccca agcaaattaa ttgaaaacca ctcacttaat ttagtcacca
     5221 aatgactatt tttactcaaa ttgtcaccat ccctaacaat tataaatcct tccttactaa
     5281 gtctgcagac tgtcttttcc agcaatctat actggtcttc tactgaaaga tagtgaagga
     5341 tgtcattcat tacaaaaacg tcacactccg gatattcata aataagtgcg tctgcatatt
     5401 caaaccttat gcgatctcct gccagataac agttccttgc tatatcaatt ttttctttat
     5461 catagtcaat ccccaatatg tgacgatctt ccgacatcaa tcccaacata aaacagagag
     5521 gaccatatcc acatccgata tctgttaatc ttgcatttag aggtatcaaa tcatgaaaat
     5581 agctgtaatt attttccatt tttatcttta ttctcatgta ccattcaagt accggacctt
     5641 tataaatata gttatgaata actgaatagt agaaatattt gttctgaggg gtatcaaaac
     5701 gttttctaag tttatcatac tcttttctca tatatgcaca cgtctccttg caaagagtcg
     5761 aataatcctt tcctgttccg gatgttaaat tacatgaaat tctcggcagt atccggtaac
     5821 cggctatacc atgtttaaca taaaatggct gctgcttgga aacgagatga ccattaccat
     5881 acattatcac cggtaaaatg tcgagagata gtttttctgc aatataaaaa gcaccttttc
     5941 taaatctgtg aatttttaag tcttttgatc gagtaccttc aggaaaaata accacagaat
     6001 atccgttttc tgtcatcttc ttaacttgtt caatatgatt ctcgatacca tcttgatagt
     6061 agataaatcc ggcatatctc acaatgtgcc caaacacagg agagttccaa acccatctgt
     6121 ttgtcatcat aataatattt ggatttaatg aaagcatcat tagtatatca atgaatgact
     6181 gatggtttgc tattataatt gccggttttt taaaatcttc gttataagga ttttctttaa
     6241 atatttttac tgtgggtgaa atccttacag gaagcattga tagcgcactt agcattttat
     6301 gaaaaacctt ttttttatat ttcacgggaa caggaataat tgcggcaatt gatatatata
     6361 ctgccgccaa aatacaccca ataacaaaaa ttgaaaaggt aatgaacatc aagagaatgc
     6421 ctgacactgt atatggagga agagatcttc tgacaggata ggtaataaaa tgtctgaaga
     6481 gtaccggttg aactgtaagg gccacaaacc atactgccat catccccagt gtagatatag
     6541 ttgcaattga cctgagagca ggatgcttgg caaatattaa aacccccatt ccgacaatga
     6601 ctgtaaaagt cgaaaaaaat atagcatttt tgtgagatga taaaatggta tttcttttgg
     6661 catattcacc ctgtaatcca tccagtacaa atatgctgaa gtcatcccca ataccaaaaa
     6721 tgaaggtgga caggaggatg ttgacaatat tgaacttaat accacagatt gccatcattc
     6781 caagaatgat aatccagctg acaaacattg gaagaaatgc cagaagagcc agttctatcc
     6841 ttccgtatga aacaagtaaa gttaaaaata caagtagcga agagagagta agaataaaat
     6901 agaaatcatc tttaacagaa gtcgcccata atcctgcaaa ataagctcta tcgacaataa
     6961 cagactgtgt ttctgaagag attttattat aaacattaga tttcacctcc tcatcaacaa
     7021 atagttgagt gataaaaact gatgaagaat ctgataactc acaccattca ctcagcaatt
     7081 tgggaaaatc tgttttatgg ctttgatagt caaatacttc gtattttttg tcaattaagt
     7141 cgtaaaaagc ttgaaaagcc tcatcggaaa aacctatttt ttttgattca gaggatatta
     7201 aatgttttac aactgaactt ttttcttgcc agtattgatt ccatctgtca attctctctt
     7261 gctggataga atgaggaatt accagagaag ctactgacga cttttttatt tcaatccctt
     7321 ctcttctcat taaatcgaga atggaatcac actgtc
//
LOCUS       DAOTQL010000086         7336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8512_length_7336_cov_4.654168, whole genome shotgun sequence.
ACCESSION   DAOTQL010000086 DAOTQL010000000
VERSION     DAOTQL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8512_length_7336_cov_4.654168"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="P5293_05925"
     CDS             <1..1096
                     /locus_tag="P5293_05925"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HRR49475.1"
                     /translation="QFIIHDGDDIFIYTGYPWLGKNSRNTLISLPGLTLGVNADKELF
                     DRIMETYIKHERENFFNPLCNPDVPLWLFWTFQQYLRITGNKTALWKKYGELFKMIVE
                     SYMENKRESVIMHKTGLLWAERSGVATSWMDTMIDGKPLMERSGYQIELNSLWYNALC
                     FAAEIAPKSKERPFIEKCLTIKELIDDNFKKAFWIEGADYLADYVGPEGQNRYVRPNQ
                     LFACAFDYSPINDEDKSLVLQCIKRELLTIRGIRTLSPRNPNYKGEYDGDEYSRSMAY
                     YQGTTRVWLLQFYIDANIKLFGNSFLNKAKELIYAFEEDLTVHCVGSISEVYDGDPPH
                     QPHGCISYSTSVAGLLWSVKLIEELKNSLV"
     gene            1093..2379
                     /locus_tag="P5293_05930"
     CDS             1093..2379
                     /locus_tag="P5293_05930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR49476.1"
                     /translation="MKVLMFGWEFPPHISGGLGTACYGLTKGLAKHGVEILFVMPSPY
                     GDEDQSAVKIINASDVVINESVKNSESFLQKVQFLRVGSNMVPYVDPVRFTTLVESQR
                     VSQIEGYKMYAGERYQFSGKYETNLMEEVARYAIVAGEIALKYDFDLIHAHDWLTYLA
                     GITAKRLTGKPLVVHMHATEFDRSGNNINTQVYDLEKMGMKEADIVIAVSNLTRSIVI
                     SKYGINPEKVVTVHNAVEFDKSGNVEVDRGVPEKVVTFLGRITYQKGPEYFIEAAAKV
                     LKRYPNVRFVMAGNGDLLTRAIRRVAKLGIATKFHFTGFLKGSDVNRMYAYSDVYVMP
                     SVSEPFGISPLEAMRANVPVIISKQSGVAEVLTHALKVDFWDIDAMADAIHALLIYPA
                     LSNFVVKNGFEEVNALKWDNAAAKVKEIYTKLTGKL"
     gene            2391..3599
                     /locus_tag="P5293_05935"
     CDS             2391..3599
                     /locus_tag="P5293_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRR49477.1"
                     /translation="MEKSVCMYFQVHQPHRLRQYRFFDIGKDSHYYDEFTNKTILRRV
                     SDSCYLPADKVIHDIIEESKGAFKIAFSLSGSVIDQFEKYAPEVIDSFKKLAETNCVE
                     FLAETYNHSLASLASPAEFKAQVMLHSKKIESLFGKKPVVFRNTELIYSDEIGAMVAD
                     MKYKAMLTEGAKHILGWKSPNYVYTNAINPKLKLLLRNFNLSDDIAFRFSERSWSNWP
                     LTADKYVTWIANTLAKDEVVNIFMDYETIGEHQKSSTGIFQFMKFFPKALLLQTDIKF
                     CTPSEICKKHQPVAPLNVPFPISWADEERDTTAWLGNELQNEAFTKLYSVEKQVLRSK
                     DTDLINDFRKLQESDHFYYMCTKFFSDGIVHGYFNPYESPYEAFINYMNILSDFLLRV
                     DKSKKTTKTS"
     gene            3609..>4634
                     /locus_tag="P5293_05940"
     CDS             3609..>4634
                     /locus_tag="P5293_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRR49478.1"
                     /translation="MSDRLLLTPDYLFEISWEVCNKIGGIHTVIATKALNLSKEFKNR
                     HILIGPDVWRDTVKNPDFNEDNNLLRAWRAKAADEGLRIRIGRWNVAGRPIAILVDFS
                     SFITKKDEILTEFWTRFKLDSITGQWDYIESALFGYACGKVIESFVKFNLSPKNRAVA
                     QFHEWMTGAGILYLKQANTPVATVFTTHATVVGRSIAWNNLPLYGPLENYVADDKARE
                     FNVMAKHSLEKTAAHFADVFTTVSEVTARECRIFLGRNVDIVTPNGFEKSFTPPVNKY
                     EEYFKSARKRLTEIATLMTGFEITDDTVMMAISGRYEFKNKGIDLFLDAAAKLNNKTG
                     SKTKAASA"
     assembly_gap    4635..4644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4645..>7336
                     /gene="glgP"
                     /locus_tag="P5293_05945"
     CDS             <4645..>7336
                     /gene="glgP"
                     /locus_tag="P5293_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRR49479.1"
                     /translation="NVVAWIFVPAAINGPNKDLINKMISHSDYITNCSHLLSEPEWDP
                     VLNRIRELNLQNRDNDRVKVLFVPAYLNGNDGIFNYTYYQLLAGLDITLFPSYYEPWG
                     YTPLESLAFGVPTVTTSLAGFGRWVSEHYKKPHPSIEIIERNDFNYDDVISAIEAKMV
                     EICKLTPQKMLDYRNNAREVSEVALWENQIQFYKQAYSQALEKLIENWDNYESFADDK
                     ESIIYKIDVANTPSWSTMIVNKVLPERLKPLDIMAKNLWWSWNEEAKQLFRSIDLDLW
                     RKCSRNPMNMMDNITASKYAALENDFDFLERMDKAYSSFNKYMSLKDEMKGPRIAYFS
                     MEYGLDVSLKIYSGGLGILAGDFLKEASDKMVPVTGVGLLYKYGYFTQRLSAQGDQIS
                     IYEPQDFTKIPVNPVRDLNGNWATISIAFPGRTLYARLWKVEIGRTELILMDTDFEDN
                     LPEDRVVTHYLYGGDWENRLKQEILLGIGGIRALRTLNKQIDIYHCNEGHAAFTGLER
                     LREYVQEEHLSFNEALEVVRSSSLFTTHTPVPAGHDSFSEDLLREYIPHIPERLKISW
                     ETMMSLGKIDPNNPNERFSMSNLAANLSQEINGVSMLHGEVSREIFAPLWPGYLPQEL
                     HVSYVTNGVHYPSWAAREWKRIYEDVFGQRFIRHIYEKSCFEGIYKVSDEDIWDTRNR
                     LRERLITLIRHTLSEPATTAHYRPKQIVTIKETLRSDVLTIGFARRFATYKRASLLFR
                     DLDRLEEIVNNEERPVQFLFAGKAHPADKAGQDIIKKIVEVSLMPQFIGKIVFIPGYD
                     MALAKTLVQGVDVWMNTPVRPLEASGTSGQKAAMNGVMHFSVLDGWWVEGYKEGAGWA
                     LPLDTTYDDQSLQDELDAANIYNILESEIIPLFYKRETRNE"
BASE COUNT         2346 a         1230 c         1599 g         2151 t
ORIGIN      
        1 acagtttatt attcatgatg gagatgatat ttttatttat acgggatatc cgtggcttgg
       61 gaaaaacagc cggaacacat tgatttccct accggggttg acacttgggg taaatgcaga
      121 caaggagttg tttgatagaa taatggagac ttatatcaag cacgaacgag aaaatttttt
      181 taatccgctt tgtaatccag atgttccact ctggcttttc tggacttttc agcaatattt
      241 aagaattact ggcaataaaa cagcattatg gaaaaagtac ggagagctgt ttaagatgat
      301 tgttgagtca tatatggaga ataaaagaga atctgttatc atgcacaaaa caggtttact
      361 ttgggctgag cgaagtggag tggctacaag ttggatggat acaatgattg atggaaaacc
      421 attgatggaa agatcaggat atcagataga gcttaattct ttgtggtata atgcactgtg
      481 ttttgctgca gaaattgctc ccaagagtaa ggagagacca tttattgaaa aatgtttgac
      541 aataaaagaa cttattgacg acaattttaa aaaagccttt tggatagaag gtgctgatta
      601 tcttgcggat tatgttggcc ctgaggggca gaacaggtac gttagaccta accagttgtt
      661 cgcatgtgct tttgattact caccaataaa tgatgaggac aaatctcttg ttttgcagtg
      721 tataaaaaga gagttgctta ctattagagg gataaggaca ctttctccaa gaaatcctaa
      781 ctataaaggc gaatatgatg gagatgaata ttcaaggagt atggcatatt atcagggtac
      841 taccagagta tggcttcttc agttttatat cgatgcaaac ataaaactgt tcggaaattc
      901 ttttttgaat aaggcaaaag agttgatata tgcttttgag gaggatttga ccgtgcattg
      961 tgttggttca atatcggaag tctacgatgg agatccccct catcagccac acggctgtat
     1021 atcttattca acatctgtag ccggtttgtt atggtcggtc aagttgattg aagaattaaa
     1081 aaacagctta gtatgaaagt attaatgttt ggttgggagt ttcctccaca tatttccgga
     1141 ggtcttggaa ctgcatgtta tggtctaaca aaaggcttag ccaagcatgg tgttgaaata
     1201 ctttttgtaa tgccttcgcc ttacggagat gaagatcaaa gtgctgtcaa aatcattaat
     1261 gcaagtgatg tggttataaa tgagtctgtg aagaacagtg agagttttct gcaaaaggtg
     1321 cagtttctgc gggtgggatc aaatatggtt ccatacgtcg atcctgttcg ttttacaact
     1381 ttggtagaat cgcagagagt cagtcagata gagggttata aaatgtacgc aggggagaga
     1441 tatcaatttt cgggcaagta tgagacgaac cttatggaag aggttgcacg atatgccata
     1501 gtggctggag aaattgcact caaatatgat tttgacttaa ttcacgctca tgattggtta
     1561 acctatctgg ccggtattac tgctaaaagg cttacaggca aaccattagt tgtacatatg
     1621 catgcaactg aatttgacag gagcggcaat aacatcaata cacaggttta cgatcttgaa
     1681 aaaatgggca tgaaggaggc agatattgtt attgctgtta gtaatcttac caggagtata
     1741 gtcataagca agtacggaat caatcctgaa aaagttgtta cagtacacaa tgctgttgaa
     1801 tttgataaat caggcaacgt tgaagtggat agaggggtac ctgaaaaggt agtaactttt
     1861 ctcggcagaa ttacttatca gaaaggtcct gaatacttta ttgaagctgc cgccaaagtc
     1921 ctgaaacgtt accctaacgt tcgttttgta atggctggga atggggatct tcttacacga
     1981 gccataagga gagttgccaa actcggcata gcaaccaaat ttcattttac aggctttctt
     2041 aagggatcag atgttaacag aatgtatgca tattcggatg tatatgtgat gccttcagtg
     2101 tctgagcctt ttgggatttc acctcttgaa gcaatgagag caaatgtccc tgtaattatt
     2161 tcaaagcagt caggcgttgc cgaggttcta acgcatgctt taaaagtaga tttttgggat
     2221 attgatgcaa tggcagatgc aattcatgca ttgcttatat atccggcact ttcaaatttt
     2281 gttgtaaaaa atggttttga agaggtaaat gcactaaagt gggataatgc tgctgcaaaa
     2341 gttaaggaga tatataccaa attaacagga aaattgtaat agataaaaag atggaaaaga
     2401 gcgtttgcat gtattttcaa gttcatcagc cacacaggct gagacagtac agattctttg
     2461 acattggtaa ggattctcat tattatgacg aatttaccaa taagacaata ctccgtcgag
     2521 tctctgattc gtgttatctt cctgctgata aagtcataca cgatataatt gaagagagta
     2581 agggcgcctt taaaattgcc ttttcacttt ccggttctgt tattgatcag tttgagaaat
     2641 atgctcctga agtaattgat agttttaaga aactggctga aacaaattgt gtggaatttc
     2701 ttgcagaaac atataaccat tctttagcct cacttgcctc tcctgctgag tttaaggcgc
     2761 aagtgatgct gcattccaaa aaaatagagt cattgtttgg taaaaaacct gttgttttca
     2821 gaaatacaga gctaatatat tctgacgaaa ttggtgcaat ggtcgcagat atgaaatata
     2881 aagccatgct gactgaagga gcaaaacaca ttctcggatg gaaaagtcct aattatgttt
     2941 atactaatgc catcaatcca aaattgaaac tgttattgag gaattttaac cttagtgacg
     3001 acattgcttt cagattttct gaaaggagtt ggtctaactg gcctcttact gccgataaat
     3061 acgttacatg gattgcaaat acactggcaa aagatgaagt tgtaaacatt tttatggatt
     3121 atgagactat aggagaacat caaaaatcgt caaccggtat ttttcaattt atgaaatttt
     3181 tccccaaggc tcttctctta caaacagata taaaattttg tactccttcc gaaatttgta
     3241 aaaaacatca gccggttgcg ccattaaacg taccttttcc tatttcttgg gcagatgaag
     3301 agagagatac aactgcatgg ctcggcaatg aacttcagaa tgaagccttc accaagctat
     3361 actctgtaga aaaacaagtt ttgaggtcaa aggacactga tttaattaat gacttcagaa
     3421 aattgcagga gagtgatcat ttttattaca tgtgtacaaa atttttctct gatggtatag
     3481 ttcatgggta ttttaatcca tatgagtctc catatgaggc gtttattaat tatatgaata
     3541 ttttgagcga ctttttgctc agggtagata aaagcaaaaa aacaacaaaa acaagttaat
     3601 tattttatat gtcagacaga ctacttttaa cccctgacta tctttttgag ataagttggg
     3661 aggtttgtaa taagattggg ggcattcata ctgttatcgc caccaaggct ctcaatctat
     3721 ctaaagaatt taaaaacaga cacatcctta taggtcctga tgtttggaga gatactgtca
     3781 aaaatccaga tttcaatgaa gacaacaact tattaagagc atggagagca aaagcggcag
     3841 acgaaggatt gcgaatcagg ataggaagat ggaatgtagc aggacgtccc atagcaatac
     3901 ttgtagattt tagttcattt attacgaaaa aagatgaaat tcttacggaa ttttggacaa
     3961 gatttaagct cgattcgata actgggcaat gggactatat agaatctgca ttgtttggat
     4021 atgcctgtgg caaggtgata gaaagttttg tcaaattcaa tctttctccc aagaacaggg
     4081 ctgtagcaca atttcatgag tggatgacag gtgccggcat tctttatctt aaacaggcaa
     4141 atacgcctgt ggcaacagtt tttacaaccc atgcgacagt tgtgggacgt tcaattgcat
     4201 ggaacaatct tccactatat ggacctcttg agaattatgt tgccgatgac aaagcaagag
     4261 aatttaatgt aatggccaaa cattctctcg agaagactgc cgcacacttt gcagacgttt
     4321 ttactactgt cagtgaagta acagcaagag agtgcagaat ttttcttggt agaaatgttg
     4381 atatagtaac tcctaacgga tttgaaaaaa gttttactcc accggtcaac aaatatgaag
     4441 aatattttaa gagtgctaga aaaagattaa ctgagattgc gactctcatg acaggttttg
     4501 aaataacaga tgatactgtt atgatggcta tcagtgggcg ttacgaattc aagaacaaag
     4561 gaatagattt atttctcgat gctgcggcta agctaaacaa caagaccgga tcaaagacaa
     4621 aagccgcttc ggctnnnnnn nnnnaaacgt agtagcatgg atatttgtcc ctgctgctat
     4681 aaatggacct aacaaggatc ttataaataa aatgatcagt cactctgatt atattacaaa
     4741 ttgttctcat ctgttaagtg aaccagaatg ggatcctgta ctgaacagaa tacgagaact
     4801 taatcttcaa aacagagata atgacagggt aaaagtactg tttgtacctg catatcttaa
     4861 tggtaatgac ggtattttta attatactta ttatcagtta ctcgccgggc tcgatattac
     4921 actttttcct tcctattacg agccttgggg ttacacacct ttggaaagcc tcgcatttgg
     4981 tgtccctact gttaccacct ctcttgcagg ctttggacgt tgggttagtg aacattataa
     5041 aaagccacat ccaagtattg agattattga aagaaatgat tttaattacg atgatgtaat
     5101 tagtgccata gaggccaaaa tggtcgaaat atgcaaactg acaccccaaa aaatgctgga
     5161 ttatcgcaat aatgcaaggg aggtctctga agttgccttg tgggagaatc agattcagtt
     5221 ctacaaacaa gcttattcac aggctttgga gaagctgatt gaaaattggg acaactatga
     5281 aagttttgca gatgacaagg aaagtataat ttataaaatt gacgtagcaa atactccaag
     5341 ttggagtaca atgatagtta acaaggtttt gcctgaaagg ctaaagccgc ttgatatcat
     5401 ggcaaaaaat ttatggtgga gctggaatga agaggccaaa caacttttca ggtctataga
     5461 tttggattta tggcgaaaat gctcaagaaa tcccatgaat atgatggaca acattaccgc
     5521 cagtaaatat gctgcactcg aaaatgactt tgatttcctg gagagaatgg acaaagcata
     5581 ttcaagcttt aacaagtaca tgtcacttaa ggatgagatg aaagggccga gaattgcata
     5641 tttcagcatg gaatatggac ttgatgtttc acttaaaatc tattcgggag ggctcggtat
     5701 cctggcggga gactttctta aggaagcaag tgacaagatg gtacctgtta caggagtagg
     5761 cttgctgtat aaatacggtt attttactca gaggctttct gctcaaggag atcagatttc
     5821 catctatgaa cctcaggatt ttacaaagat acctgtcaat cctgtaaggg atttaaatgg
     5881 aaactgggcg acaataagta ttgcttttcc tggaaggaca ctttatgcta gactttggaa
     5941 agtggaaatt ggcagaacag agcttatact catggatact gattttgaag ataatttacc
     6001 agaggacaga gtcgttacgc actaccttta tgggggagat tgggagaaca gactcaaaca
     6061 ggaaattttg ctcggtatcg gtggtatacg tgcacttagg acactaaaca aacagatcga
     6121 tatttaccac tgcaatgagg gccatgctgc attcacaggt cttgaaaggc ttagagagta
     6181 cgtacaggaa gagcatcttt catttaatga agcccttgaa gtagtgaggt cttcttcact
     6241 ttttactacg catactcctg tccctgcagg ccatgattca ttttcggaag atcttctcag
     6301 agagtatatt cctcacattc ctgaaagact caaaatctca tgggaaacca tgatgtctct
     6361 tggcaagata gatccaaaca atccaaatga aaggttttcc atgagtaacc ttgccgccaa
     6421 tctgtcacaa gagattaatg gtgtcagcat gcttcatgga gaagtcagtc gtgaaatatt
     6481 tgctccctta tggccaggct atcttcctca ggagcttcat gtaagctatg tgacaaacgg
     6541 ggttcactat ccttcatggg ctgcaaggga atggaaaagg atatacgaag atgtttttgg
     6601 acagagattt atccggcata tatatgaaaa gagttgcttt gaaggaatat acaaagtttc
     6661 tgacgaagac atttgggata caagaaacag attgagagag aggcttataa cactaattag
     6721 acatactctt tctgaacctg ccactactgc tcattacaga cccaaacaaa ttgtaacaat
     6781 aaaagagaca ctcaggtcag atgtgcttac aattggattt gccagaagat ttgcaactta
     6841 taagagagcc tctttgcttt tcagagattt ggacagactg gaggaaatcg ttaataatga
     6901 agagcgacca gttcagtttc tttttgcagg caaagctcac ccggcagaca aagccggtca
     6961 ggatattatt aagaagattg ttgaagtatc acttatgcct caatttattg gaaaaatcgt
     7021 atttattccc ggctatgaca tggcattggc aaaaacgctt gtacagggag tagatgtttg
     7081 gatgaatact ccggtaagac ctcttgaagc ttctggtaca agtggacaga aagcagccat
     7141 gaatggtgtc atgcattttt ctgttcttga cggatggtgg gtagagggct acaaagaagg
     7201 agcaggatgg gcactgcctc ttgacactac ttatgacgac cagtctcttc aggatgagct
     7261 cgacgctgcc aatatatata atatacttga atctgaaata atccctttgt tctataaaag
     7321 agaaacgaga aacgag
//
LOCUS       DAOTQL010000087         7331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8524_length_7331_cov_4.926883, whole genome shotgun sequence.
ACCESSION   DAOTQL010000087 DAOTQL010000000
VERSION     DAOTQL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_7331_cov_4.926883"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5293_05950"
     CDS             <1..552
                     /locus_tag="P5293_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330220.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRR49480.1"
                     /translation="GEISYACEVVVGRYKAITRMNTGVYSSEIKNDSDFEKAKMLVAE
                     FAKKEGRQPRIMVAKLGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPEEAAKQAVEN
                     DVHVIGISSLAAGHKTLVPQLINELKKLGRDDIMVIVGGVIPPQDYDALYKEGVAAIF
                     GPGTPISVAAIKLMELLMQKFEQ"
     gene            complement(640..2214)
                     /locus_tag="P5293_05955"
     CDS             complement(640..2214)
                     /locus_tag="P5293_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRR49481.1"
                     /translation="MKTIRLIRQTVAMICILICCSAPLYSQEISSARLDSLVENTMKS
                     FNVPGIAVAVVKDNRIVHMKGYGVSSIITGKKTDENTLFAIASNSKAFTAAALAILVD
                     EGKIEWDTHVTDIIPEFKLYDPYVTNEFTIRDLLTHRSGLGLGAGDLMLWPDGTLFTL
                     NDIIYNMRYLKPVSSFRTKYDYDNLMYLVAGEVVSRVSGMSWEDFIEDRIMTPLGMGR
                     SAACYSRIKDKSNVIDAHIYTHGKVQRIPMNFTPVGNAAGGIFSNISDLSKWVMMQLN
                     GGKYGDNLSKSIFSQKVGREMWSPQTIIPASSYDYKSNFAAYGLGWRVNDINGYKQVS
                     HTGGLSGVVTQVTMIPELKLGIIVLTNQQASGAYVTITNTIKDTYLGIKGRDRLKENI
                     EALRRNSSSARDEVSKVWNKVASSAKFAPGSEDLKSYTGKYRDNWFGIVNITLENGAL
                     RFASEMSPQLKGTLCFYTGDTFVVKWDERSFDADAFVWYSKNIEGVPSGFKMKAISPE
                     TDFSYDFQDLDFYRVK"
     gene            2273..2776
                     /locus_tag="P5293_05960"
     CDS             2273..2776
                     /locus_tag="P5293_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRR49482.1"
                     /translation="MDTFLIILGLIFTLTGLLGCLLPAIPGPPLSFIALLLVHLTKEA
                     DLSNSLLLAMAVLTIVVMVLDYIIPLWGTKKFGGTKFGTWGATIGLIIGFFLGPLGII
                     IGPFAGAVLGEMVNGSSGSRSLRAGIGSFLGFLMGIGLKLVVSGIMTFYYFKYSLPVI
                     KNLISTL"
     gene            complement(2760..3623)
                     /locus_tag="P5293_05965"
     CDS             complement(2760..3623)
                     /locus_tag="P5293_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRR49483.1"
                     /translation="MEIKSSSSIQLLTYEYVRTLMRERKADSSKRDYGHALIIAGSFG
                     MMGAAVLASRGCMRSGVGLLTAHVPSCGYQIMQISIPEVMCSVDSSSDCFSVPPPIEK
                     FDAIAVGPGIGRAESTVRATEILLRSKPARLVIDADAINILASNRDLLELLPEQTIFT
                     PHPGEFSRLMEVKIDDNNKIDMQRQFSEKYHAIVVLKGHETTISAPDGRLFKNTTGNP
                     GMATAGSGDVLTGVILALRAQGYSASEAACIGVFLHGLAGDFAVMNKSEYSLISGDIA
                     EYLSSAFKELI"
     gene            complement(3614..4495)
                     /locus_tag="P5293_05970"
     CDS             complement(3614..4495)
                     /locus_tag="P5293_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRR49484.1"
                     /translation="MVRLKFLFFSVLTTAFYTQLFSQQVKDLLPEWTFTKGIEGPFFD
                     SKGNLYAVNFGEQGTVGVVTPDGKASCYLHLPEGSIGNGIIFDSKGFMYIADYTGHNI
                     LKVDPSSKKIEFFAHEPSMNQPNDIAISPITGFIYASDPNWKDSTGKLWMIDLTGKVL
                     LLEDNMGTTNGIEVSPDGHRLYVNESVKRLIWVYDILSDGVVSGKRLFYKFTDFGMDG
                     MRCDKWGNLYVTRHGKGSVVILSPKGKMLKEVFLKGKLCSNIALKNADSEITAFVTMA
                     DRGCFEYFTIKMPDEWK"
     gene            4814..5833
                     /gene="metX"
                     /locus_tag="P5293_05975"
     CDS             4814..5833
                     /gene="metX"
                     /locus_tag="P5293_05975"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRR49485.1"
                     /translation="MNRYFYKYNSPFELECGEKLEQLEICYHITDNFTTDKKVIWICH
                     ALTANSNPEEWWPNFVGKGKLFDTDKYSVICANILGSCYGSTGPASFDQNGNQYLLSF
                     PNITVRDIVRAHDILRERLGIRHIDIVIGGSIGGFQALEWSIMFPEKIAGMVLLACNA
                     KVSPWGTAFNETQRMALFSDPSFEEQNSISGGAKGLETARAIALLSYRSYKGYKLTQS
                     EEDCDKLFAEKACSYQKYQGKKLKDRFNAYSYYTLTKSIDSHNVGRGRGGVEKALSQI
                     KAKPLIIGIDSDVLFPLEEQKFLAENIPNSQFEIISSDFGHDGFLLEWKSITAAIKKH
                     IKIIQ"
     gene            5835..>7331
                     /locus_tag="P5293_05980"
     CDS             5835..>7331
                     /locus_tag="P5293_05980"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRR49486.1"
                     /translation="MQVLKFGGSSVADAANISNVTKIVKENLKRDRTILVASAIGGCT
                     DKLIEIGKLAAMQDETFKTLIDELENRHLQIILELFPVDFQKSISIIVREIFHQLRDV
                     CKGVYYLKELSNSSLDLIMSFGEILSTKIISERFTSMGINNLWADARELIKTEPCNNG
                     NSVIKLVSYANIRKMTNSSRLRLIIVPGFIASDTLNHTTTLGRGGSDFTAALVAEAVE
                     ARELQIWSDVNGMMTADPRIVPEAITIRHISYKEAQELSHFGAKVIYPPTIQPAISKG
                     IPIVVKNTFSPDDPGTVIEKNPPESRGKIRGISRSNKIALLSMEGSGMVGVPGYSARL
                     FGALANAGINIILITQASSVHTMCVAIEDSDAAKAKKTVDETFAYEISLNKVNPLKVE
                     NGFSIISLVGDDLKNQSGTGGRMFNALGRAGINIRAIAQGSSEKNISVVVKEEESDIS
                     IKVIHDEFFGVEKKRINLFIAGYGNVAKSLLSIIKSRREWLIEKRHLEI"
BASE COUNT         2285 a         1454 c         1379 g         2213 t
ORIGIN      
        1 ggtgagatat catatgcatg tgaagttgtg gtcggaagat ataaagcaat tactcgtatg
       61 aacactggcg tatattcaag cgaaattaaa aatgacagcg attttgaaaa agcaaagatg
      121 cttgtcgctg aatttgcaaa aaaagagggg cgacagcctc gtattatggt ggccaaactt
      181 gggcaagatg ggcatgacag aggagctaaa gttgtagcca caggttatgc agactgtgga
      241 tttgatgtcg atatggggcc tctcttccaa acacctgaag aggcagccaa acaagctgtt
      301 gaaaacgatg tgcatgtaat tgggatctct tctcttgcag ctggtcacaa aacattggtc
      361 cctcaactta taaatgaact gaaaaaactt ggaagagatg atattatggt aatcgtcgga
      421 ggtgtaatac ccccacaaga ttatgatgca ctctacaaag aaggtgttgc agctatattt
      481 ggtcctggaa caccaatctc tgtcgctgct atcaaactca tggaactgct tatgcaaaag
      541 tttgaacagt aatagttttt agcagtaata gcagacaaaa ggtcgccttg acaaacaaaa
      601 tgtttcggcg accttttttt cgattattat tttagattat tattttaccc tgtaaaaatc
      661 taaatcctga aaatcataac taaagtctgt ctcaggagat atggctttca tcttaaatcc
      721 tgaaggtacc ccttcaatat ttttagagta ccacacaaaa gcatctgcgt caaaacttct
      781 ttcatcccat ttcacaacaa atgtgtcgcc tgtataaaaa cagagtgtac ctttgagctg
      841 tggagacatt tcagatgcaa atctcaatgc tccgttttct aaagttatat taactatccc
      901 aaaccagttg tctctgtact tgcctgtata actcttcaaa tcttcgctcc ccggagcaaa
      961 tttggctgaa cttgcaactt tgttccacac tttgctaact tcatcacgag ctgacgaaga
     1021 atttctgcga agagcctcta tgttttcttt caacctatct cttcccttaa tccccagata
     1081 agtatcttta atagtattag ttatagtaac ataggcgcca gatgcctgtt gattggtaag
     1141 cacaattatt ccaagtttaa gctccggaat catagttacc tgagtaacca cacctgaaag
     1201 tcctccggta tgtgaaacct gcttgtatcc atttatgtca ttgactctcc atcccaatcc
     1261 atatgcagca aagttagatt tatagtcata acttgaggca ggaattatcg tctgtgggct
     1321 ccacatctca cgtccgacct tctgagaaaa aatagattta gataaattat ctccgtattt
     1381 tcctccattc agctgcatca ttacccattt gcttaagtct gatatgttgc tgaaaattcc
     1441 acctgcagca ttaccgacag gtgtgaaatt catcggaatc ctttgcactt tcccatgagt
     1501 atatatatga gcatcaatga cattactctt gtcttttatg cggctgtaac atgctgcact
     1561 cctgcccatt ccaagaggag tcataattct gtcttcgata aaatcttccc aactcatgcc
     1621 agagactctt gaaacaactt ctccggcaac caaatacata agattatcat agtcatactt
     1681 agttctgaaa gaagagacag gcttaagata tctcatatta taaattatgt cattcaaagt
     1741 aaatagagta ccgtccggcc agagcataag atctcctgct ccaagtccca aaccacttcg
     1801 atgtgttaga agatctctta ttgtaaactc attggtgaca tacgggtcat aaagtttaaa
     1861 ttcaggaatt atgtctgtta catgtgtgtc ccactcgatt ttaccttcat ccaccagtat
     1921 ggcaagagct gcagctgtaa atgccttgct gttggaagca atagcaaaaa gtgtattctc
     1981 atcagtcttt ttcccagtga taattgaaga gacaccatat cctttcatat gcacaattct
     2041 gttgtcttta acaactgcaa ctgctattcc cggaacatta aacgatttca tagtgttttc
     2101 aacaagactg tcaagcctgg cagaagatat ctcctgtgaa taaagaggtg ccgagcaaca
     2161 gatcaaaatg cagatcattg ctacagtttg tcttattaga cgaatggttt tcatatttga
     2221 gtattttagt tattgcaaga tatttttttt aaattcgcat aaacagataa aaatggatac
     2281 tttcctaatt atattgggac tgatttttac tcttacaggt cttttaggtt gtttacttcc
     2341 ggcaatacct ggtcctcctc tttccttcat tgcacttctc ctggttcact taaccaagga
     2401 ggcagatctc agcaattctc ttttgcttgc tatggctgta ctgacaatag tggtaatggt
     2461 attggactat ataataccac tctggggaac aaaaaaattt ggaggcacaa aatttggcac
     2521 atggggagct acaataggac tgataatagg attttttctt ggtcctttag gtataataat
     2581 cggtccattt gcaggtgctg tcttgggaga aatggtcaac ggctcttcag ggagcaggtc
     2641 tcttcgtgca ggaattggat catttttggg tttccttatg ggaattggtt taaaattggt
     2701 tgtgtccgga atcatgactt tttactattt taagtactct ctcccagtta taaaaaatct
     2761 catatcaact ctttaaatgc actcgaaagg tattcggcta tatctcctga tattagagaa
     2821 tactcacttt tgttcattac cgcaaaatct cctgcaagac catgcaaaaa tacaccgatg
     2881 catgctgcct cagatgcgga ataaccttga gcgcggagag caagaatgac tcctgtaaga
     2941 acgtccccac tgccagctgt ggccatacct ggattacccg ttgtgttttt gaacagtctt
     3001 ccatcaggtg cagatattgt agtttcgtgt ccctttagaa ccactattgc atgatacttt
     3061 tccgaaaatt gacgctgcat gtctattttg ttgttgtcat caatctttac ttccataagc
     3121 ctcgaaaatt cgccgggatg aggcgtaaaa attgtttgtt caggaagtag ttcaagaaga
     3181 tctctgttgg atgcaagaat attaatagca tctgcatcaa tcactaacct ggctggtttg
     3241 gatctcagta agatctctgt tgctcttaca gttgattctg ctcttcctat cccgggacct
     3301 actgcaattg catcaaattt ctcaattggt ggaggaactg aaaaacaatc agaagaagaa
     3361 tcgacactac acatgacttc aggaattgat atttgcatga tttgataacc acaggatgga
     3421 acatgtgcag ttagtaaacc aacacctgag cgcatacatc cccgagatgc caggactgcc
     3481 gcacccatca ttccgaaaga tccggctatt ataagtgcat gtccataatc tctcttgctt
     3541 gagtctgctt tccgctctcg catgagagtc cttacatatt catatgtaag caattggata
     3601 ctgctgctag attttatttc cattcatcag gcattttaat ggtgaaatat tcaaaacaac
     3661 ctctgtcggc catagtcaca aaagctgtaa tttcgctgtc tgcattttta agagctatgt
     3721 tagagcataa ttttccttta aggaagactt ccttaagcat tttccctttc ggagacaaaa
     3781 ttactacact acctttccca tgtctcgtaa cataaaggtt tccccattta tcacaccgca
     3841 tgccgtccat gccaaaatca gtaaatttat aaaacaacct tttaccagaa actacgccat
     3901 cagataatat gtcataaacc caaattaatc ttttgacgga ttcatttacg taaagcctgt
     3961 gaccatccgg gcttacctca atcccgttag ttgtacccat attgtcttcg agtaaaagta
     4021 ctttacctgt cagatctatc atccataatt tcccggtaga atctttccaa ttgggatcgc
     4081 tggcatatat aaatcccgta ataggagata tggctatatc gttcggctgg ttcatggagg
     4141 gctcatgcgc aaaaaattca attttttttg atgatggatc tacctttaat atattatgtc
     4201 ccgtataatc agctatatac ataaaccctt tactgtcaaa tattattcca ttacctatag
     4261 acccctcagg cagatgcaaa taacaagaag ccttcccatc tggagttact acaccaactg
     4321 tgccctgttc gccaaaatta actgcataga gattgccttt tgagtcgaaa aatggtcctt
     4381 caatgccttt tgtaaaagtc cattcaggaa gtaaatcttt gacctgctgc gaaaatagtt
     4441 gtgtataaaa agctgttgtt aaaacagaaa aaaataaaaa ctttagtctt accatttcaa
     4501 aaatgttatt ataatattga aattatcaac aataatagta caaaaaattt gtcatttatt
     4561 caacaatttc tacttttgca aatcgaataa atgtcatgag tagagatttc aatcatataa
     4621 tttcttcttc aagagtgtct tatgacacgc tttctactct gattttgatg tcatccatga
     4681 tgatgcgaat gcatcgcgag atgctcggtt aatttcagga tcttatgcag gtcccttggg
     4741 tgtaagccca aaaatccaat aacattttca attttttata gtgctaaagc tcgaattctt
     4801 gtgcatgaaa accatgaaca gatattttta taaatataac tctccttttg aattagagtg
     4861 tggagaaaag cttgaacaac ttgaaatttg ctatcacata acagataatt ttacaacaga
     4921 taaaaaagtc atttggatat gccatgctct tactgcaaac tctaatcctg aagaatggtg
     4981 gccaaatttt gtaggaaaag gcaaattgtt cgatactgac aaatactctg tgatttgcgc
     5041 caatatactt gggtcttgtt acggaagcac tggacctgct tcttttgacc aaaacggtaa
     5101 tcagtatctc ctctcttttc caaatattac tgtaagagat atcgtacgtg ctcacgacat
     5161 tctaagagaa cgtcttggaa tcagacatat agatatagtc atcggtggct caattggagg
     5221 atttcaggct ctcgaatgga gtatcatgtt ccctgaaaaa attgccggca tggtattgct
     5281 ggcatgcaat gcaaaagtct ctccatgggg gacagctttt aatgaaactc aaagaatggc
     5341 acttttttct gatccttctt ttgaggaaca aaacagtatc agtggaggag ctaaaggcct
     5401 tgaaacagca agagctatcg ctctactatc ttaccggtct tataaaggtt acaagttaac
     5461 tcaaagtgaa gaagattgtg ataaactatt cgcagaaaaa gcttgcagtt atcaaaaata
     5521 tcagggtaaa aaactgaaag atagatttaa tgcctactcc tattacaccc taactaaatc
     5581 tattgacagc cataatgttg gaagaggaag aggaggcgtt gaaaaggctc tttcacaaat
     5641 taaggctaaa cctttaataa taggaattga cagtgatgtg cttttcccat tggaggaaca
     5701 gaaatttctc gctgaaaaca tcccaaactc tcaatttgaa ataatatcat cagattttgg
     5761 acatgatgga tttttgcttg agtggaaatc aattacggca gcaataaaaa aacatattaa
     5821 aattattcag taaaatgcaa gtactaaaat tcggaggaag ttcggttgca gatgcagcca
     5881 atatctccaa tgtaacaaaa atcgtcaagg agaacttgaa aagagacaga accattcttg
     5941 ttgcatcggc aattggagga tgcactgata agctcatcga aatagggaag cttgcagcaa
     6001 tgcaagatga aactttcaaa actttgatag atgagctgga aaatagacat ttgcaaatca
     6061 tcctcgaact ttttcccgta gattttcaaa aatcaatatc gataatagtc agagagatat
     6121 tccatcagtt aagagacgta tgtaaaggtg tgtactatct caaggagttg agcaattcct
     6181 cgcttgatct tataatgagt tttggagaaa tattatccac aaaaattatc agcgaacgct
     6241 ttacctctat gggtattaac aatctatggg ctgatgcaag agaactcatc aagacagaac
     6301 cctgcaataa cggtaacagc gtcataaaat tggttagtta tgctaatata cgaaaaatga
     6361 ccaattccag tagattaaga cttattattg ttcccggatt tatcgcaagc gatactttga
     6421 atcataccac aactttggga cgaggtggat ctgactttac cgcagcactg gttgctgaag
     6481 cagtagaagc aagagaatta cagatttgga gtgacgtcaa cggtatgatg acagccgatc
     6541 cgagaatagt tccggaagca atcactataa gacacatttc atataaagaa gcacaggagt
     6601 tgtctcactt cggggcaaaa gtgatttacc ctcctaccat acagccggct ataagcaaag
     6661 gtattcccat agtggttaaa aacacttttt ctcccgatga tccgggcact gttattgaaa
     6721 aaaatccgcc tgaaagcaga ggaaaaatta gaggaatatc aagaagcaac aaaatagctc
     6781 ttctctccat ggaaggcagt ggaatggttg gcgtaccggg atattctgca agactatttg
     6841 gtgctctggc caatgctgga ataaatatta tattgattac acaagcttct tcggtacata
     6901 ctatgtgtgt ggccattgaa gactctgatg ccgcaaaagc caaaaaaact gtggatgaaa
     6961 cctttgctta tgagatatcc ctcaacaaag tcaatccatt gaaagttgaa aacggattct
     7021 ctataatctc tcttgttgga gatgatctta agaatcagtc cggcacagga ggtagaatgt
     7081 tcaatgcact tggacgtgcc gggattaata tacgggctat tgcacaaggt tcttccgaaa
     7141 aaaacatctc cgtagtggtc aaggaagagg aatcggacat ctctattaag gtaattcatg
     7201 atgaattctt tggagttgaa aagaagcgta ttaatctttt tattgcagga tatggaaatg
     7261 ttgcaaagtc ccttctttca ataataaaat ctcgcagaga atggctcata gaaaaacgac
     7321 atttagagat a
//
LOCUS       DAOTQL010000088         7244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8636_length_7244_cov_5.163166, whole genome shotgun sequence.
ACCESSION   DAOTQL010000088 DAOTQL010000000
VERSION     DAOTQL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_7244_cov_5.163166"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="P5293_05985"
     CDS             complement(<1..215)
                     /locus_tag="P5293_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49487.1"
                     /translation="MKKLYMLFAIIILLQFTSCTRSKCELDYLPEKIDSKGTLVIEGG
                     GDRIAVILQTIIKYGGGDSAKVLVVPFA"
     gene            complement(217..1323)
                     /locus_tag="P5293_05990"
     CDS             complement(217..1323)
                     /locus_tag="P5293_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRR49488.1"
                     /translation="MKGLDERLKQIFVELTSINAVSGEEAPVADYITAFAERLGLTCQ
                     RDNAYELSGGNTGNVIIEIMGGGEFLMASHMDTPRPIIGLKRQFFEDRITSDGTTPLG
                     VDDRAGLSSILFALENAVKEGKLEPCTLLFTVCEETTLAGSIYFTPKPQLKYGFIFDS
                     YLTPGHFVSQTCGAINFEFVIKGKSAHAGISPEKGINAIKVAAEAITKFPFGWVDEVT
                     NANIGSVAGGKNTNVVCDEVHMKGELRTDFVSHGDKLMTNILKDFNDVCHNYGATMES
                     KYFWDFLPYNITADDLPFKHFAQIAASLGLEAVPKKSMGGSDANALNAKGIKTINLGV
                     GAQNPHGNDEFILYEDFQKASLIAYKLLTTKFNK"
     gene            complement(1337..2380)
                     /locus_tag="P5293_05995"
     CDS             complement(1337..2380)
                     /locus_tag="P5293_05995"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HRR49489.1"
                     /translation="MKNSILKITLLSLVAVFSVSCQKEDWTYKGPQYYEFSAYENHQG
                     TVSNILQKENSKIGLDSICIQIIKPADGDVTVNYEIVDKVYYLTDKDRYVVEIPEGTD
                     ISVVDTVYSTAVLGTDFEIVPATGQTFSAASKSGSLVISKGNYFGYIQVKMLRKSGTN
                     FFVVLKDSPDTKANKPTSILNYKLAPDKIFYFQESFLDEIPDTWTLIDRDGDGYNWNY
                     YDGAATSDSWLSGGVGAVRPENYLISPKIEIGNTTDVVLLTFEVAAGDTDYPEENYRV
                     IISENPITYENCRQATILRDWTELDETYGDFKLQTIDISAYKGKDVYIGFVHGNCTDC
                     YYMLLRNVKVFGQ"
     gene            complement(2410..3840)
                     /locus_tag="P5293_06000"
     CDS             complement(2410..3840)
                     /locus_tag="P5293_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR49490.1"
                     /translation="MKNFKLLISISLLFVLNSCDSLLDISTKHALPQDKVKSIEGCEG
                     LIIGVYDLLQDPYYAGRDLICVPDVMGDNCEPSPSSTKYAGQYNFQPYYNIDIWESAY
                     KQIGALNEAILYLSDMEQTDKVKSLMGEALFLRAYNYFNLAIIYSRVPGHLVDNFNLC
                     VPLLTEPFFNEGGSITEQASVPRATVDEVWAQIVLDLSNSFTLLENNDNKKYPYRISA
                     VAVKAFQSRVYLYKGDWANAVIAANYAITNSPVTIYTGKYTDIFSQGKESLWQLHFTI
                     SENLASTSLHSTYGTVDNGYRDEEGFGDGTGAGDAQLSVTQDFVDLIDQQRDTRFQAL
                     RKVHYYGQNLWWTTKFNSWGGTFGLDDIPLIRISEVYLNRAEAYAHQNNYTSARNDLN
                     YLMNKRGLDNTSVANAELLNEILLQRRIELAFEGHRFFDMKRLGKPISRPNGMPQIPY
                     DDYRVVAPISTTELDVNKKLVNNPGY"
     gene            complement(3852..7178)
                     /locus_tag="P5293_06005"
     CDS             complement(3852..7178)
                     /locus_tag="P5293_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49491.1"
                     /translation="MKEDKRSKVSFVVLLLFLINVPFVNIGAQNQKDLYNVKLDNLTL
                     KEAMVKITSQCGYYFVYEDADIANVPKISKDFKSSSIEQIMTECLKGTNLTFEIDKKI
                     IYIKKSTAKPATAGGLKKIAQSDDSLYVYGKITDMNNQPLPGASVEVKGVKNKGVTAD
                     YDGNYKLKLSDPSKENVLVFNYLGMQSKEVEINGRKEINVSLSEGINELEQVVVVGYG
                     VTKKKDIAGSIENISATEIAKTNTQSFQRAMQGKVSGVQIVSSSGIPGSSFSINIRGR
                     GSINADTQPLYIVDGVQITNGAQSTNILTNADVMAGLNPDDIESISILKDGASASIYG
                     AQAANGVVIITTKKGSAGKTKVSANATIGIQEIARKVPVMTGPQWAEFALLEYKNYDQ
                     YNGTNKCQQQLELFKSFGWGNDGYSNAPTTDWYDEIFRKALLNNYQVSLTGGNEKTRF
                     YASAGYNKTDGIIKHTGFNRISARLNLSHEIAPWLTLNTNNTFSGTIHNQSSVIGAAN
                     PARTAMFLLPGVSPRDENGEYYSDLTYGYFLYNIPQMLELNEYTGKTHNLLSANDLTL
                     KLAKGLEFKSSYNIDLTWLNEHQFSDPRTRLGSRVNGAVSANSTDINKFQTEQVLSYN
                     ATFNENRLSAVAGFSYSNYKYHMIGAEANGVSSPDLHLLSSAATPVSTTESYSEWKMA
                     GFFGRLSYTIKEKYIFTGTIRYDGSSRFGKDNLWGTFPSISFAWRVKEENFLKDIDWL
                     NDLKLRASYGVTGNASIGDYVSARLYEANASYGGSSGIIPSSIGNQKLSWEKKHSKNF
                     GITAGLFKGRINTNIDLYRDDTKDLLYYRVIPQTTGFSEIPSNMGGVRNQGIDLQINT
                     VNIDSGDFSWETSINLSWCKNEITELQDGLEELGQYKVGKPVTAEKVYKWAGVNTSDG
                     RPMYYDKDGYITYNPTLEDRVWTKGTDPTFFGGIDNTIKWKNFTLSFFFQFQSGAVKY
                     FNDKAVLIGQAADNNLLEEMYKSYWSKPGDVTWVPKPVLDGAYPGNPMKYDNNGDPRM
                     SLIFESTDFIKLKNVNLSYDIPAKLVKKLKLQDAQIFATGYNLWTTTPYQGYDPESVG
                     IDRGIYPQSKSYSLGIKINF"
BASE COUNT         2203 a         1517 c         1136 g         2388 t
ORIGIN      
        1 gcaaatggca ctacaagaac cttagcactg tcccctcctc catactttat tatggtttgg
       61 agaataactg caattctgtc accaccaccc tcaatgacta aagttccctt tgagtcaatt
      121 ttttcaggta aataatccaa ttcacatttt gaacgagtac aagaagtaaa ttgcaataat
      181 ataattattg caaacaacat atatagtttt ttcattttac ttgttaaatt tagttgttaa
      241 taatttgtaa gcaattaatg atgctttctg aaaatcttca taaagaataa attcatcatt
      301 tccatgagga ttttgagcac ctactcctaa attaattgtt tttatgcctt ttgcattgag
      361 cgcattagca tcactgcctc ccatcgattt ttttggaaca gcctcaagtc ccaaacttgc
      421 agctatttga gcaaaatgct taaatggaag atcatctgca gtaatattat aaggaagaaa
      481 gtcccaaaaa tattttgatt ccatagtagc tccatagttg tgacacacat cgttgaaatc
      541 tttcaatata ttagtcatca acttatcacc atgtgataca aaatctgttc ttagttctcc
      601 tttcatatgt acttcatcac agacaacatt tgtattctta ccccctgcaa cagagccgat
      661 attagcattt gtaacttcat ctacccatcc gaaagggaat tttgtgattg cctctgcagc
      721 aacttttatg gcatttatgc ccttttctgg agatatacca gcatgagcag atttaccttt
      781 tattacaaat tcaaaattta ttgctccgca agtttgtgat acaaaatgtc ccggcgtaag
      841 ataggaatca aaaataaaac catacttaag ctgtggctta ggtgtaaaat agatcgatcc
      901 cgcaagtgtt gtctcttcac aaactgtaaa aaggagtgta catggctcaa gcttaccctc
      961 tttgacagca ttttccagtg cgaaaagaat agacgaaaga cctgccctgt catccactcc
     1021 caatggggtt gtaccatccg aggtaatcct gtcttcaaaa aactgacgtt ttaatccaat
     1081 aattggcctt ggtgtatcca tgtgagaagc cataagaaat tctcctcctc ccataatctc
     1141 aataattaca ttacctgtgt taccaccaga caactcataa gcattatccc tttgacatgt
     1201 taaaccaagt ctctcagcaa atgctgtaat ataatcagcc acaggagcct cttctccaga
     1261 gaccgcattt attgaagtca attctacaaa aatttgtttt agtctttcgt ccaatccttt
     1321 cattttcaat catttattat tgtccaaaca cttttacatt tctcaacaac atataataac
     1381 agtcagtaca atttccatga acaaaaccaa tatatacatc cttgccctta tatgcagaaa
     1441 tatctatagt ttgtaattta aaatctccat aagtctcatc taactctgtc caatctctaa
     1501 gaatagttgc ttggcgacaa ttttcataag ttattggatt ttcagaaata atcactctgt
     1561 aattctcttc tggatagtca gtatcacctg ctgcaacttc aaatgtaagt aaaaccacat
     1621 ctgttgtatt tcctatttca atcttaggtg atattagata attttctggt cttactgccc
     1681 ctactccgcc agaaagccat gaatctgatg tggctgctcc atcataataa ttccagttgt
     1741 aaccgtctcc atctctgtca attaatgtcc atgtatcagg aatctcatca agaaacgact
     1801 cctggaagta gaatattttg tcaggggcaa gtttgtaatt cagaattgaa gtaggcttat
     1861 tggccttagt atcaggagaa tcctttaaaa ccacaaagaa gtttgttcca gactttctaa
     1921 gcatcttaac ctggatatag ccaaaatagt tacccttact tataacaagc gaaccggatt
     1981 ttgatgcagc agaaaatgtc tgaccagttg ctggaactat ttcaaaatca gtaccaagta
     2041 ctgcagtaga ataaacagtg tcaacaacag aaatatctgt cccttcaggg atttctacaa
     2101 catatctgtc tttatctgtt agataataaa ccttgtctac aatctcgtag ttaacggtga
     2161 catcaccatc agccggcttg ataatctgaa tacaaatgga atcaagtcca attttcgaat
     2221 tctccttttg gagaatattg ctgacagtac cctggtggtt ctcataagca ctgaattcat
     2281 agtactgagg gcctttatat gtccagtcct ccttttgaca tgatacggag aatacagcca
     2341 caagggataa taaagttatt tttaatattg aatttttcat tttcatcctt attattgata
     2401 taagcaaaat taatatcccg gattattcac aagtttttta ttgacatcaa gttctgttgt
     2461 acttatcggt gctacaactc tatagtcgtc ataaggaatt tgaggcatcc cgtttggtct
     2521 tgaaatcggt ttccctaatc tcttcatatc aaaaaacctg tgtccctcaa atgcaagttc
     2581 gatccttctt tgcaaaagaa tttcattcaa aagttcagca tttgccacac tagtattatc
     2641 aagacctctt ttattcatca gataatttaa gtcattcctt gcagaagtat aattgttctg
     2701 atgagcataa gcctcggccc tattgagata aacctcagag atccttatca aagggatatc
     2761 atcaagtcca aatgttcctc cccaactgtt aaacttagtt gtccaccaaa gattctgacc
     2821 ataatagtgc acttttctta aagcttgaaa tctagtatct ctttgttgat caatcagatc
     2881 aacaaaatct tgtgttacag acagctgagc atcacctgct cctgtgccat ctccaaatcc
     2941 ctcttcgtcc ctgtagccat tatctacagt gccataagta gaatgaaggg atgttgatgc
     3001 aagattctct gaaattgtga aatgtaattg ccacaaagac tctttacctt gagaaaaaat
     3061 atctgtatat ttacctgtat aaatagttac tggagaattg gttatggcat aatttgctgc
     3121 aataactgca tttgcccaat ctcctttata caagtatacc ctcgactgaa aagctttaac
     3181 tgcaacagca gatattctat acggatattt tttgttatca ttgttttcga gcaatgtaaa
     3241 tgaattagaa agatcaagta ctatctgtgc ccaaacttca tcaacagttg cccttggtac
     3301 agaagcctgt tctgttatac ttccaccttc gttaaaaaat ggttcagtaa gtaatggaac
     3361 acatagatta aaattatcca ctaaatgacc aggaacgcga ctataaataa tcgcaagatt
     3421 aaaatagttg taagccctca aaaaaagtgc ttcacccata agcgatttaa ctttatcagt
     3481 ctgttccatg tctgataaat aaagtattgc ctcattcaac gcacctatct gtttgtaggc
     3541 agattcccag atatcaatat tgtaataagg ctggaaatta tactgacctg catattttgt
     3601 tgagcttgga gaaggttcac agttgtctcc cattacatcc ggaacacaga ttaaatccct
     3661 tcctgcataa tatggatcct gtaacaaatc ataaactcct atgataagac cttcacaacc
     3721 ctctatgctt tttaccttat cttgaggtaa agcatgtttc gtacttatat cgagcaatga
     3781 atcacacgaa ttcaacacaa aaagtaacga aattgatatt aatagcttaa aatttttcat
     3841 tgcatgaagt tttagaagtt tatcttaatt ccaagactgt agctttttga ctgaggataa
     3901 attcctctgt caattcccac tgactcaggg tcataacctt gatatggagt tgtagtccac
     3961 aaattatagc cggtagcgaa aatttgtgca tcctgcaatt tcagtttttt taccagtttt
     4021 gcaggaatat cataactaag gttaacattc ttgagtttaa taaaatcagt agattcaaaa
     4081 ataagactca tccttggatc tccattgtta tcgtatttca ttggattgcc aggatatgca
     4141 ccatccaaaa caggttttgg cacccatgta acatcaccag gcttgctcca atagctttta
     4201 tacatctcct caagcagatt gttgtcagct gcctgaccta tcaacactgc cttgtcatta
     4261 aaatatttga cggcaccact ctggaattga aagaaaaatg ataaagtgaa gttcttccat
     4321 ttaattgtgt tgtctatacc accaaaaaat gttggatctg ttccttttgt ccaaaccctg
     4381 tcttccaagg ttggattgta tgtaatataa ccgtctttgt cataatacat tggccgacca
     4441 tctgaagtat taacacctgc ccacttatat actttctctg ctgtaacagg ctttcctact
     4501 ttatattgac caagttcttc aagaccatcc tgaagttcag ttatttcatt cttgcaccaa
     4561 gagagattta tagatgtctc ccatgagaag tcgcctgaat cgatattaac agtattaatt
     4621 tgcaggtcaa ttccctgatt tctgacacct cccatatttg atggtatttc actaaagcct
     4681 gtagtctgag gaattaccct gtaataaaga agatctttag tatcatcacg atacaaatca
     4741 atattagtat taatccttcc cttaaataat cctgctgtta ttccgaaatt tttggaatgt
     4801 ttcttctccc aagatagctt ttgattaccg attgaagacg gaattattcc agaacttcct
     4861 ccatatgaag cattagcttc ataaagtctt gcagagacat agtctcctat gcttgcatta
     4921 ccagtaacac catagctcgc tctcaacttc aaatcattca accagtcgat atccttaagg
     4981 aaattttctt cctttaccct ccatgcaaaa gaaatggacg gaaaagtacc ccaaagatta
     5041 tccttgccaa atcttgaaga accgtcgtat cttattgtgc ctgtaaaaat atatttctcc
     5101 ttgatagtgt aactcagtct tccaaaaaaa ccagccatct tccattctga ataggattca
     5161 gttgttgata caggagttgc ggcactgctt aaaagatgta aatcagggct tgatactccg
     5221 ttagcctctg caccgatcat gtgatatttg taattgctat atgaaaaacc tgcaacagca
     5281 gataatctgt tttcattaaa agttgcgtta taagatagaa cctgctctgt ctgaaacttg
     5341 tttatatcag tcgaattggc agaaacagca ccattaactc ttgaccccaa tctggttctt
     5401 ggatcactga actgatgttc attcaaccat gtaagatcaa tattgtaact gcttttaaac
     5461 tctagtccct ttgcaagttt taatgtcaaa tcattggcag atagaagatt gtgagtttta
     5521 cctgtatatt cgttcagttc aagcatttga ggaatattat aaagaaagta tccatatgtc
     5581 aaatctgaat aatattctcc attctcatct cttggagata ctccaggcag cagaaacatt
     5641 gctgttcttg caggattcgc cgcacctata actgaagact gattatggat agtaccgcta
     5701 aatgtgttat ttgtattgag agtaagccat ggtgctattt cgtgactaag attcaaacga
     5761 gctgatattc tgttaaaacc tgtgtgcttg attataccat cagttttatt atatccggca
     5821 gaagcataaa atcttgtttt ttcattgccg ccagttaaac ttacctgata gttgttaagc
     5881 agcgccttac gaaaaatctc atcgtaccag tctgtagtgg gagcatttga atatccgtca
     5941 ttcccccaac caaaactttt aaaaagttca agctgttgct gacatttatt agttccattg
     6001 tactgatcat aatttttata ttcaagaaga gcgaattctg cccattgtgg ccccgtcata
     6061 acaggcactt ttcttgctat ttcctgaatg ccaatagttg cattggcgga gacttttgtc
     6121 tttccggcag atcctttttt ggtagtaata attactactc cgtttgcagc ttgagctcca
     6181 taaattgatg cagatgcacc atccttcaaa atacttattg attcgatatc atctggatta
     6241 agtcctgcca ttacatcggc atttgttaaa atattggtag actgtgcacc atttgttatt
     6301 tgcacgccat caacaatata aagcggttgt gtatcagcat tgattgatcc tcttcccctt
     6361 atgtttatag aaaaagaaga acccggaata cctgaagacg agactatttg aactccagaa
     6421 accttgccct gcatagccct ctgaaaactc tgtgtattag ttttggcaat ttcagtagca
     6481 cttatattct caatagatcc cgcaatatct ttctttttgg tgacaccata tccaactaca
     6541 acaacttgct ccagctcatt aataccctca ctgagagaaa cattgatctc ttttcttcca
     6601 tttatttcaa cttccttgga ttgcatccca agataattaa aaaccaggac attctcctta
     6661 gaggggtcac tcagtttcaa cttgtagttt ccatcataat cagctgttac acctttgttt
     6721 ttaacgcctt tgacttcgac cgaggctccg ggcaaaggtt gattattcat atctgttatc
     6781 ttgccataaa cataaagaga gtcatcactc tgagcgatct tttttagacc acccgcagtt
     6841 gcaggttttg ccgtagattt tttgatgtaa attatctttt tatctatctc aaaagtgaga
     6901 tttgtgccct taaggcactc tgtcataatt tgttcaatac tactcgattt gaaatccttg
     6961 ctaattttag gaacattagc aatatcagcg tcttcatata caaagtaata tccacattgc
     7021 gatgtaatct taaccatcgc ctccttcaat gtaaggttgt caagctttac attgtaaaga
     7081 tctttctgat tctgcgctcc aatgttcaca aaaggaacat taatcaaaaa caggagcaac
     7141 acaacaaaag agaccttgct tcttttgtct tctttcatag tcggataagt taaatgaaaa
     7201 ttaatatata gttactgttc ttccacttag cacaatgtgc cctg
//
LOCUS       DAOTQL010000089         7235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8649_length_7235_cov_4.798189, whole genome shotgun sequence.
ACCESSION   DAOTQL010000089 DAOTQL010000000
VERSION     DAOTQL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8649_length_7235_cov_4.798189"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="P5293_06010"
     CDS             complement(<1..686)
                     /locus_tag="P5293_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49492.1"
                     /translation="MKFHEVDMMGKNWCERVSSLPTWEDSFIGRVVYNTSDGIIYYAN
                     PSKWVPLYIFKTIRVSGQDDIIADDVEDVLTLVAGDGIVLTTDPSSDALTITIAANFG
                     RISVPGQLDVVADNANDILTLASSNSGLSIFTNPTTDTITFTANYGGESFPTNPVAGM
                     MFYRTDEKYWYLRNAANTGWIKVFHEDRPPLDQRDGGIFSADLIDGYQASVIGGTLQI
                     SANNLSLIGIT"
     gene            complement(732..5978)
                     /locus_tag="P5293_06015"
     CDS             complement(732..5978)
                     /locus_tag="P5293_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HRR49493.1"
                     /translation="MSFVEKYLKPEDIAGKNILEVGSLDINGSARYYVEKHNPSKYLG
                     TDIVKGKGVDEICDVQHLAKKYGENSFDIVICTEVLEHVQDWKAAIENMISVLRTGGK
                     LIITTRSPGFPVHNYPSDYWRYTKENFRQIFSDMENVIIEDDPEKGVLFYGVKGVEMR
                     KIPNVKLDTPIPFDLVIGHCSFIPHTGYAYHAREYFTRLADHLPVRIRNFAYTPDLSH
                     LSEKQKNMVIHQTWTQPPYQLGQPFNRNFYHRIINIILLETNHYYYYENYDGPKVAYN
                     VWESTRQPEQFFQKLLEFDMMWVPTNWQRWCTIEQGYPADKIFVVPEGVNGNFFCPGE
                     PPIKVLNDERFKFLLCGRWDYRKSTAEILRAFIEEFKKDEPVDIICQIENPFKQDDYN
                     TTQERLAGHKLIDPRIKIVTGLPENDDLYLSYLRSCHCLVTCSRSEGWNLPLCVIKGS
                     EVVTSEGITPIENINVDDNVITHLGRERKVIEKFERKIKEDIISIRLWTDYEPINVTN
                     EHPILTIKREKIRGKFRKYLDKIVPEWVRASDIKKGDLIVRTTIPQKYNKNIIIDMKD
                     IDNRLLYDNDYVWYNTGYNGKGEQKKYKRFINLSDLSYVFGWYIAEGCSSDKSRVNIT
                     VNIKEKDVVIKWLEDIKEIFGAVGSYTEYPTKIQARVSSTLLSKFFSFYCGEKADQKK
                     IPYELLFGDVENLRIVIDEYAKSNGHITDRNISCSTISKELARQLVLANQRLGRKASI
                     RKSVRRENKREDFVIWWSRENENYRHSNYSWFINQGLAILVKDVKNIFYDGPVYNFEV
                     EEDESYLLCNVTVHNCQGIATGIPTIASNWGAQLEFCKDASHLVNIKELRRPENVFMQ
                     KDTPGEWAEPDYEHLKKVMREIYENYEECKKKALKQSEVVRRDFTWEKAVDKSVEILK
                     QFGSGKSKGKKVETKRLHLGCGGDIKVGYINADKFDKRADVNFDAKEIPYDDQTFDEV
                     YSSHLMEHFNKYEVPVVFKECYRILNYGGKLVFEVPDFEWVVKTWLGKTDEEKWGFHL
                     DTIFGLQTNEGEQHKVGFTRGKLKSLLAEAGFRNIVMKDIWSHDQQCIYVEAQKRHLK
                     EIFIIDTYPDLPEKEELTIGMIEEVKDRGYPVLLVSHYPISTDIQKMVNYYVYDANNI
                     MSDGWNLNYWFQNEEVKIVSQYETKYHGAACYSSLVNAVRVLSQYDIAHFIEFDIDAD
                     LDLYLSEAGKKLEKYKLVGFMYDEEQNTRKVAGMEPTYGIISNLFSFDIKWMNERMKD
                     ISSWSDYNKVIAEASEKTKIRIDLIFENWLYNYFKANADWKDIFLFTKNDMKYKIIKE
                     RNLFDQGKKEPIERVFLSETADNKVVQFTVNTETKKWSYKIVEREENKDYTKTNFKFK
                     DGRLICKRWNPSDDKGWMESEDKIAVTFIDGVKVEITGNSSSEYDVGFFDKDVNLQIH
                     RGKIKPNHWIAPNARYYVNWQIMINKDGKPYKSYELDLVNNKVLVYFDSKALGDTLAW
                     FPYAKIFKEKHGCKNFYVSTFWNRIFEKEYPDLKFVEPGQFRPDIYYNVGCRDNDYHS
                     NKNNWRLVPLQKVATDYLGLEYEEVRPKVTAEKKNVKKPYVTISEHSTLLCKRWHYPL
                     GWQTVVDALNDRGYGVMVVSKEPTQLKNIIDRTNSTIQQTINNIYNSRLFIGVGSGLS
                     WLAWALNIPVILISGFSEPWSEMNDCIRISPPEGICRGCYNDIKHPYDRGNWTWCPRN
                     KKYECSRTIKPEVIIEQIDKIL"
     gene            complement(5997..6392)
                     /locus_tag="P5293_06020"
     CDS             complement(5997..6392)
                     /locus_tag="P5293_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49494.1"
                     /translation="MLQKLSVGLLILGILLFANVSFAEEVCLSEENAAKIVVELERAN
                     IMKEELQILKDQNAELYKQVKLMKEVVDLQQQQLEASKKTVEVLQETIKAQGEAYEKQ
                     LKQSKPNMMYQILEYAGFLGAGILIGLLL"
     gene            complement(6337..6702)
                     /locus_tag="P5293_06025"
     CDS             complement(6337..6702)
                     /locus_tag="P5293_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49495.1"
                     /translation="MTNVWEFIKSFKAVLIIAVTIGGLVFGGAYAYKNWGAVPGLLEG
                     IFSDRLQEISDQYEAELKIKETQIESYKIQLNKSQAELKKLRTKIKELEAKIQNVQMP
                     TNVAETKRRLTDLGYPPVR"
     gene            complement(6708..>7235)
                     /locus_tag="P5293_06030"
     CDS             complement(6708..>7235)
                     /locus_tag="P5293_06030"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRR49496.1"
                     /translation="RCDDFIPDVRAQHIRYFGPRASWWYGIGQLRQESNCRAKATAWD
                     QGMGIAQFMPATAKEINRLMKTNLDPYNPEHAIRMQAYYMSSLHRQNWDEQKRLWLTY
                     QAYNGGYGNLKSEYRRAGKVDWLAMKENCKRRKVTLKSGQVLDFCEVNYDYSVKVHKY
                     GKVYRSQNIDQIKFW"
BASE COUNT         2048 a         1558 c         1083 g         2546 t
ORIGIN      
        1 gttattccta tcaatgatag attatttgct gaaatctgta gtgttccacc aattactgat
       61 gcctgatatc catcaatcaa atcggcactg aaaattcctc catccctctg atcaagcggt
      121 ggtctatctt cgtgaaatac ttttatccat ccggtattgg ctgcgtttct caaataccag
      181 tatttttcat ctgtcctata gaacatcatc ccggctacag gatttgttgg aaagctttca
      241 ccaccataat ttgcagtaaa tgtaattgta tcagttgtgg ggtttgtaaa gatgctcaat
      301 ccgctattgg atgatgcgag ggtaagaata tcattggcat tatcagctac tacatcaagt
      361 tggcccggca cagaaattct accaaaattt gccgctattg taatagtcaa tgcatcgctt
      421 gacgggtctg tggtcagaac aataccatca ccggcgacca atgtaagaac atcttcaaca
      481 tcgtcggcta taatatcgtc ttgtcctgat accctgattg ttttgaaaat ataaagagga
      541 acccacttgg aaggattggc atagtaaata ataccgtcgc tggtattata aaccactcta
      601 ccaataaaag agtcttccca agttggaagg gatgaaaccc tctcacacca gttttttccc
      661 atcatatcta cttcgtgaaa tttcatttct tatcccccga ataaaagaat tatacaatat
      721 ttctcttata tttatagaat tttgtctatt tgttctataa taacttcagg ttttatggtt
      781 cgcgaacact catatttttt atttcttggg caccaagtcc aatttcccct gtcatatgga
      841 tgttttatgt cattgtaaca tcctctgcag attccttccg gtggagaaat tcttatacaa
      901 tcattcattt ctgaccacgg ctcggaaaaa ccggagataa gaatgacagg tatattcaat
      961 gcccatgcaa gccaagagag accggaacca actccaatga aaagtctcga attgtagata
     1021 ttatttattg tttgttgaat tgttgagttt gttctgtcaa tgatattttt taattgtgta
     1081 ggctctttac tgacaaccat tacaccatat cccctgtcat tcagggcatc aacgactgtt
     1141 tgccagccaa gagggtaatg ccacctttta cagagaaggg ttgaatgttc tgaaattgtt
     1201 acatacggtt tcttgacatt ctttttttct gctgtaacct ttggtctcac ttcctcgtat
     1261 tcaaggccga gataatccgt tgcaactttc tgtaatggaa ccaatctcca attgttttta
     1321 tttgagtgat agtcattatc ccggcatccc acattataat agatatcagg tctaaattga
     1381 cccggctcta caaatttcaa atccggatat tccttctcaa atatcctatt ccagaatgtt
     1441 gacacataaa agtttttgca accgtgtttc tccttaaata tctttgcgta ggggaaccaa
     1501 gccaatgtat cacccagcgc cttactgtca aaatatacaa gaaccttatt gttcacaaga
     1561 tcaagttcat atgatttgta cggcttccca tccttattaa tcattatttg ccagtttaca
     1621 tagtatctcg cattcggagc aatccaatga ttcggtttta ttttacccct atgaatttga
     1681 agatttacat ccttgtcaaa aaagcctaca tcgtattctg aactgctatt accagtaatc
     1741 tccaccttta caccatcaat aaaggtaacc gcaattttat cttcagactc catccatccc
     1801 ttgtcatcag acggattcca tcttttacag attaatcttc cgtccttgaa cttgaaattg
     1861 gttttggtat agtccttatt ctcctccctt tctactattt tatatgacca tttttttgtt
     1921 tcagtattga cagtaaactg gacaacttta ttatccgctg tttcagatag aaatactctt
     1981 tctatcggct ccttttttcc ttgatcaaat aaattccttt ccttgatgat tttatatttc
     2041 atatcatttt tggtgaatag aaaaatatct ttccaatcgg catttgcctt gaaataatta
     2101 tacaaccagt tctcaaaaat caggtcaatt ctgatcttgg tcttttcgga ggcctctgca
     2161 attactttgt tgtagtcact ccaagatgaa atgtccttca tcctttcatt catccatttt
     2221 atatcaaatg agaaaagatt agagataatt ccgtatgtcg gttccatacc ggctactttt
     2281 cttgtatttt gttcctcatc atacataaag ccgaccagtt tgtatttttc aagcttcttc
     2341 ccagcttcgg agaggtaaag gtcaagatca gcatcaatgt caaattctat aaaatgcgca
     2401 atatcatatt gagagagcac tctaacggca tttaccagtg atgaataaca ggcggccccg
     2461 tggtatttcg tttcatattg ggaaactatt tttacttctt cattttggaa ccaatagttg
     2521 aggttccaac catcactcat aatattattt gcatcataaa catagtaatt caccattttc
     2581 tgtatatcag tagaaattgg atagtgagat acaagaagaa caggatatcc cctgtctttt
     2641 acttcttcta tcattcctat ggtaagttct tccttttcag gcaagtctgg ataggtatca
     2701 ataatgaaaa tttctttcag gtgccttttt tgtgcttcga cataaataca ttgctggtca
     2761 tgtgaccaga tatctttcat tacgatattt ctgaatcctg cttccgcaag aagactttta
     2821 agttttcctc ttgtgaaacc aactttatgc tgttcacctt cattggtctg caagccgaaa
     2881 atagtatcaa gatgaaatcc ccatttttcc tcatcagttt ttccaagcca ggttttcaca
     2941 acccactcaa aatccggaac ctcaaaaaca agttttccac cataattcaa tatacggtaa
     3001 cattctttga aaacaaccgg cacttcatac ttgttaaaat gctccatcaa atgtgaagaa
     3061 tagacctcat caaaagtttg atcatcataa ggaatttctt tcgcatcaaa gttcacatcg
     3121 gctcttttat caaacttgtc tgcattgata tatccgactt taatgtcacc accacaaccc
     3181 aagtgtaatc tttttgtctc aactttttta cccttacttt ttcccgatcc aaattgtttg
     3241 agaatttcaa cggatttatc aactgccttc tcccaagtaa aatctcttct cactacttca
     3301 gattgtttga gggctttctt tttacattct tcataattct cataaatttc cctcattact
     3361 ttcttgagat gttcatagtc tggctcggcc cattcgcccg gcgtgtcctt ctgcataaaa
     3421 acattttcag gccttctcaa ttccttgatg tttaccaaat gggatgcgtc cttacagaac
     3481 tccaattggg ctccccagtt tgatgcgatt gttggtattc ctgttgcaat tccttgacaa
     3541 ttatgaacag tgacattaca taatagataa ctttcatcct cttccacttc aaaattataa
     3601 accggcccat cataaaaaat atttttcaca tcctttacaa ggatagccaa tccctgattt
     3661 ataaaccaag aataattact gtgtctataa ttttcatttt ctctactcca ccaaataaca
     3721 aaatcttctc ttttattttc tctccgaaca gactttcgaa ttgatgcctt tcttcctaat
     3781 cgttggtttg ccaatacaag ttgtcgtgcc aattcttttg atattgttga acaggaaatg
     3841 tttctatctg ttatgtgccc attactcttt gcatattcat caataacaat tcgaagattt
     3901 tcaacatctc caaataacaa ttcataagga attttctttt ggtcagcctt ttcaccacaa
     3961 taaaaagaaa aaaactttga caggagggta gaactaactc ttgcttgaat ttttgttgga
     4021 tactctgtat aacttccaac agcaccaaaa atctctttta tatcttcaag ccactttatt
     4081 acgacatctt tttcttttat atttaccgtt atattcactc ttgatttatc tgaagaacat
     4141 ccttcggcaa tataccaacc aaatacataa gataagtcag ataaatttat aaatcttttg
     4201 tattttttct gttcgccttt accattatat ccggtattat accatacata atcattatca
     4261 tataataatc tattatcaat atctttcata tcaattatga tatttttatt atatttctgt
     4321 ggaatggttg ttcttacaat caaatccccc ttctttatgt cagatgccct cacccattca
     4381 ggaacaattt tatccagata ttttctgaat ttccccctga ttttttctct ttttattgta
     4441 agtatgggat gttcattcgt tacatttatt ggctcataat ctgtccataa acggatggaa
     4501 ataatatctt cttttatttt tctttcaaat ttttcaatga cttttctttc tcttccaaga
     4561 tgtgttataa cattatcatc tacatttata ttctcaattg gtgttattcc ttcggatgtt
     4621 acaacttcag atccctttat aacacacaaa ggaagattcc atccttctga tcttgaacag
     4681 gtcacaagac aatgacaaga acgaagataa gaaagataaa gatcatcatt ttccggaagc
     4741 cccgtcacaa tttttattcg tggatcaata agtttatgtc cggctaatct ttcctgtgta
     4801 gtgttgtaat catcctgttt gaatgggttt tcaatctgac aaataatatc gacaggctca
     4861 tctttcttga actcttcaat aaaagctctc aaaatttctg cggtagattt tctataatcc
     4921 catcttccac aaagaagaaa cttaaacctt tcatcattca atacctttat tggtggctcg
     4981 ccaggacaga aaaaattacc attaacacct tccggtacaa caaatatctt atctgccgga
     5041 tatccctgtt ctatcgtgca ccatctctgc caatttgtgg gaacccacat catatcaaat
     5101 tcaagtagtt tttgaaaaaa ttgctctggc tgccttgtgc tctcccatac attgtaggca
     5161 actttcggcc cgtcataatt ctcataatag taataatgat ttgtttcaag tagaataatg
     5221 tttatgattc tatggtaaaa atttcgattg aatggttgac ccaattgata tggcggctgt
     5281 gtccaagtct ggtgaatgac catattcttc tgtttttcag aaagatgtga caagtctggt
     5341 gtgtatgcaa aatttcttat tcttacggga agatggtctg caagtcgagt aaaatattct
     5401 cttgcgtggt aggcatatcc agtatgggga ataaagctac aatgaccaat gaccaaatca
     5461 aaaggaatgg gtgtatcaag tttcacattt ggaatttttc tcatctctac tccctttaca
     5521 ccataaaata aaacaccctt ttcgggatca tcttctataa tgacattttc catatcggaa
     5581 aaaatttgtc tgaaattttc ctttgtatat cgccagtaat ctgatggata gttgtgaact
     5641 ggaaagccgg gtgatcgggt agtgataata agcttgccgc ctgtacggag aactgaaatc
     5701 atattctcta tggcggcctt ccaatcttga acgtgctcaa gaacttctgt gcatattaca
     5761 atgtcaaaac tattttcacc atactttttg gcaagatgtt gaacatcaca tatttcatca
     5821 acacctttac cctttacaat atcagttcca agatactttg acggattatg tttctcgaca
     5881 taataccttg cagagccatt tatatcgagg gaaccaactt caaggatatt tttaccagca
     5941 atatcttccg gtttcaaata tttttcaaca aacgacaaac atgcttgatt acacatttat
     6001 aataataacc cgataagtat tcctgctcca agaaaaccgg cgtactccaa tatctgatac
     6061 atcatattgg gcttggattg tttcagctgt ttctcgtaag cttctccctg tgctttgatg
     6121 gtttcctgta aaacttcaac tgttttcttg gatgcctcca attgctgttg ttgtaggtca
     6181 acaacttcct tcatcagttt tacttgttta tacaattcgg cattttgatc cttgagtatc
     6241 tgaagttctt ccttcataat attggctctc tcaagttcga caactatctt tgcagcattt
     6301 tcctctgata aacagacttc ttctgcaaaa gaaacattag cgaacaggag gatacccaag
     6361 atcagtaagc ctacgcttag tttctgcaac atttgtgggc atctgaacat tttgtatctt
     6421 tgcctccaat tcttttattt tagtacgaag tttctttaat tctgcttgtg acttgtttaa
     6481 ttggattttg taactctcaa tttgtgtttc cttgattttc aattctgctt catattggtc
     6541 acttatctcc tgtaacctat cgctgaagat accttcaaga agaccgggaa ctgctcccca
     6601 atttttatag gcataagcac caccaaaaac aaggccacca atggtcacag caataatcaa
     6661 taccgctttg aaagacttta taaattccca aacatttgtc ataataatca ccaaaatttt
     6721 atttgatcta tattttgact tctataaacc ttaccatatt tatgaacctt cacagagtaa
     6781 tcatagttca cttcacaaaa atcaagtacc tgaccactct tgagggtcac ttttcttctt
     6841 ttacagtttt ctttcattgc gagccagtca accttaccgg ctctcctgta ttcagatttc
     6901 agatttccat atccaccatt gtatgcttga tatgtcaacc acaatctctt ttgttcatcc
     6961 caattttgcc tatgaaggga tgacatataa tatgcctgca ttctgatggc gtgttccgga
     7021 ttgtagggat caaggttcgt tttcatcaga cgatttattt cttttgcggt tgctggcata
     7081 aattgtgcaa tacccatccc ctgatcccac gccgtagctt ttgctctaca gttactttcc
     7141 tgtctcaatt gtccaatacc gtaccaccaa gaagcccgag ggccgaaata cctgatatgt
     7201 tgcgctctca catccggtat gaaatcatca catct
//
LOCUS       DAOTQL010000090         7225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8663_length_7225_cov_4.147141, whole genome shotgun sequence.
ACCESSION   DAOTQL010000090 DAOTQL010000000
VERSION     DAOTQL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8663_length_7225_cov_4.147141"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="P5293_06035"
     CDS             <1..1537
                     /locus_tag="P5293_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRR49497.1"
                     /translation="VSDLQVSDVILVKPGERIPVDRVVVEGSSFVDESAITGESIPVE
                     KLPGTKVVSGSVNGNGSFRFRAERVGKETLLSQIIRVVRESQASKAPVQRIVDKVASV
                     FVPVVVAASLLTFILWMLLGGTDLFPRALLSAISVLVIACPCAMGLATPTALLVGIGK
                     AAQNHILIKDAAALERTRMVDTVVLDKTGTITTGQTEVTDWIWLEDNSVLTGGFTKEE
                     FMNVIVAMERRSEHPLAGAIINFLEDKTEKSNIDVESFENIPGQGVTAIFKDHLFWAG
                     NERMVSEKSIVESANGPLSKCENLKSEGKSILFLGIDNICVAIVAVADSVSESSVEAL
                     RSLREMGIEVNMLTGDNVIVATNIAAKLGITHFLAGVLPSEKEEYVIKLQKEGRIVAM
                     VGDGVNDSQALAVADVSIAMRKGTDIAMDVAMMTLMTSDLRLLPAAFAISERTVRLIH
                     QNLFWAFFYNIVGIPIAAGVLYPFYGITLNPMIAAAAMAFSSISVVLNSLRLGKFNYH
                     PKG"
     gene            complement(1519..2466)
                     /locus_tag="P5293_06040"
     CDS             complement(1519..2466)
                     /locus_tag="P5293_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR49498.1"
                     /translation="MGVAGYIAPRHLKAIKNSGNTLVSCVDPFDSVGLLDSYFPECSY
                     FNQFELFDRHLAKLQSAGQGIDYLSVCTPDYLHDAHIRYGLKIGANVITEKPMVLNPK
                     NLDDLELVEKETGKKVYTILQLRYHDVILGLKKQIDEGPKDKVYNIDLNYTTSRGSWF
                     FTSWKGNPLQSGGIATNIGIHFYDMFQWIFGKVTTNIVHIKTHDRVAGLLEFEKARVR
                     YFMSINSDTLPPEIRATGQRIYRAITVDGNTFDFTDGFADLHTESYKKILEGEGFGID
                     DARPSIEIVHDIEASKPIGLKGDYHPFAKLPLASHPFGW"
     gene            complement(2514..3515)
                     /locus_tag="P5293_06045"
     CDS             complement(2514..3515)
                     /locus_tag="P5293_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRR49499.1"
                     /translation="MKNFALIGAAGYVAPRHMKAIKDTGNNLIAAYDINDSVGIVDSY
                     FPKCAFFKQYELFERHLSKKRAFSNTGDLEKDYPKGKLDYVSICTPNYLHDAHIRFGL
                     REGADVICEKPIVLNPWNIDALEFAEKETGHKIYNILQLRLHDAIIALKKRVDEGPKD
                     KIYDVDLTYITSRGNWYYTSWKGDPRKSGGVATNIGVHFYDMLGWIFGDVKQNIVHIS
                     SHDRVAGYLEFDKAKVRYFLSINVNTLPNEIRESGKRTYRSLTMEGEEVEFSEGFTEL
                     HTASYRHILEGKGFGLEDARKCIQIVHDIRNATPIGLKGDYHPFARLQQTPHPFGWK"
     gene            complement(3556..4611)
                     /gene="rfbB"
                     /locus_tag="P5293_06050"
     CDS             complement(3556..4611)
                     /gene="rfbB"
                     /locus_tag="P5293_06050"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRR49500.1"
                     /translation="MRHILITGGAGFIGSHLIRLMVNKYPEYSIVNLDKLTYAGNLSN
                     LKDIENKKNYTFIKGDICDFELVRSIFSEMNIDGVMHLAAESHVDRSIKDPFSFAQTN
                     VMGTLSLLQAAKLAWENNFENKLFYHVSTDEVYGSLNDNISLFKETSRYEPHSPYSAS
                     KAASDHFVRAYHDTYGLPIIISNCSNNYGSYQFPEKLIPLLINNIRHNQPLPVYGKGE
                     NIRDWLYVEDHAAAIDLIFHKGKVGETYNIGGFNEWRNIDLVKLIVKIVDRELGREDG
                     SSEKLITYVTDRAGHDLRYAIDSTKLQTELGWSPSVQFEDGLTKTVRWYLSNQEWLDS
                     VTSGDYRRYYDEMYRQS"
     gene            complement(4608..5492)
                     /gene="rfbD"
                     /locus_tag="P5293_06055"
     CDS             complement(4608..5492)
                     /gene="rfbD"
                     /locus_tag="P5293_06055"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRR49501.1"
                     /translation="MTGTLVTGSNGQLGNELRAIENKLPGTVYYTDVEELDICQKESV
                     IQFIKKNNIDLIINCAAYTAVDKAEDEPAAAYQLNAEAPGFLAEAARENNALLLHIST
                     DYVFNGKGPKPYKESDITAPVSVYGKTKLAGEKAIENSGCRYLILRTSWLYSIYGNNF
                     VKTIVRLAKEKKELKVVFDQTGTPTFAADLALAISEISAHKDELFYIKNAENHIYNFS
                     NEGVCSWYDFAVEIIKFTSNTLPIHPVRSEEFPSKATRPAFSVLDKTLIKKVYGINIP
                     HWRDSLEKCMKIILQTRL"
     gene            complement(5496..6062)
                     /gene="rfbC"
                     /locus_tag="P5293_06060"
     CDS             complement(5496..6062)
                     /gene="rfbC"
                     /locus_tag="P5293_06060"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRR49502.1"
                     /translation="MNFIRTAIPDVYIIEPIVHGDGRGYFMESFLLSEFEREISKISF
                     VQENESCSKYGVLRGLHYQAEPFAQSKLIRVVTGRIMDIAVDIRKGSPTFGRYMAVEL
                     SGENKRQLFIPKGFAHGFSVLEDNTIVQYKTDNIYSPSHERAILWNDPSLAIKWGIPA
                     KDVILSDKDKKHPLLKDAYLFEYKDDRQ"
     gene            complement(6059..6940)
                     /gene="rfbA"
                     /locus_tag="P5293_06065"
     CDS             complement(6059..6940)
                     /gene="rfbA"
                     /locus_tag="P5293_06065"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRR49503.1"
                     /translation="MKGIVLAGGSGTRLYPITKGVSKQLLPIYDKPMVYYPISVLMLA
                     GIREILIISTPDDLPGFCRLLGDGSDFGVHFEYAEQPSPDGLAQAFIIGEKFIGQDSA
                     CLVLGDNIFYGQFFSRMLKNAVKSAEESSEATVFGYWVNDPERYGVAEFDKTGKVLSI
                     EEKPTNPKSNYAVVGLYFYPNKVVKVAKTIKPSDRGELEITTVNQEFLKEKKLNVQLL
                     GRGFAWLDTGTHDSLSDASTFVEVIEKRQGLKIACLEEIAYKNGWINDKKLIEIARPM
                     IKNQYGQYLINLTKKRK"
     gene            complement(6952..>7225)
                     /locus_tag="P5293_06070"
     CDS             complement(6952..>7225)
                     /locus_tag="P5293_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49504.1"
                     /translation="LEEAVEYCKSRGLEFYAVNENHPDCTGNDSPRKLIADMYIDDRN
                     FGGVPDWGFIYNALKYKPDECFSPEIFQQNEEQLPKTKKRGLFRHK"
BASE COUNT         1940 a         1353 c         1512 g         2420 t
ORIGIN      
        1 agtatcagat ttgcaggttt cagatgttat acttgtcaaa cctggcgaaa gaattcctgt
       61 agatagggtt gttgttgagg gtagttcttt tgtggatgaa agtgccataa caggtgaatc
      121 tattcctgtt gagaaattgc ctggaacaaa ggtcgtttcg ggaagtgtga atggaaatgg
      181 ttcttttagg tttcgtgcag aaagggtagg caaagaaact cttctttctc agattatacg
      241 agttgtcaga gagtcacagg cgagtaaggc tcctgttcaa agaattgtgg acaaggtggc
      301 gtctgttttt gtgcctgtgg tagtggcggc atctctgctt acatttatat tatggatgtt
      361 gttgggagga acagatcttt ttccgagggc tttattgtct gcgatatctg ttctggtgat
      421 tgcgtgtccc tgtgcgatgg gcctggcaac tccaactgca ttacttgttg gaataggcaa
      481 ggctgctcaa aatcatattc ttattaaaga tgctgcagct cttgaaagaa ctagaatggt
      541 ggatactgta gttttagaca aaactggaac tattactaca ggccaaactg aagttactga
      601 ttggatttgg ctggaagata attccgtttt aacagggggt tttaccaaag aagagtttat
      661 gaatgttata gttgccatgg agagaagatc tgaacaccct ctggcaggag ctatcataaa
      721 ttttcttgag gataagactg aaaaatcgaa cattgatgtc gaatcttttg aaaatattcc
      781 tggacaggga gtgactgcta tttttaaaga tcatcttttc tgggcgggaa acgagaggat
      841 ggttagtgag aaaagtattg ttgaatctgc aaatggacct ctttcgaaat gtgaaaactt
      901 gaaaagtgaa gggaaaagta ttttattttt gggtattgac aatatctgtg tggcgatagt
      961 ggctgtggca gatagtgtga gcgaaagttc tgtcgaggct ttgagaagtt taagagagat
     1021 ggggattgag gtgaatatgc tcacgggaga taatgtaata gttgctacga atattgctgc
     1081 aaaactgggt attacacatt ttcttgcagg agttcttcct tcggagaaag aagaatatgt
     1141 cattaaatta cagaaagagg gaagaatagt tgccatggtt ggcgatggag taaatgacag
     1201 tcaggcgctt gcagttgccg acgtaagtat tgcaatgaga aagggtactg atatagcgat
     1261 ggatgttgcc atgatgactc taatgacttc agacttgcga cttttgccgg cagcatttgc
     1321 aatttctgaa aggactgtaa ggcttataca tcaaaattta ttctgggcat ttttctacaa
     1381 tattgtagga ataccaatag ctgctggggt tctgtatcca ttttacggaa taacactgaa
     1441 ccccatgatt gcagcagcag ccatggcgtt cagctctatt tcagtggttt tgaacagtct
     1501 ccgattaggc aagtttaatt accatccgaa aggatgactt gcgagaggta gtttggcaaa
     1561 cgggtgataa tcacctttca gtcctatagg tttggatgcc tcaatatcgt gaactatttc
     1621 gatggaagga cgggcatcat caattccaaa tccttcaccc tcgagaattt tcttgtagga
     1681 ttctgtatgt aaatctgcaa agccgtcagt gaaatcgaat gtattgccgt caacagtaat
     1741 agctctgtaa attctctgac cggtagctct tatctcggga ggcaatgtgt cggagttgat
     1801 gctcatgaag tatcttacac gagccttttc aaactcgagg agaccagcaa ccctgtcatg
     1861 tgttttgata tgaacaatgt ttgtggtcac tttgccaaat atccattgga acatatcgta
     1921 aaaatgtatt cctatattgg tagcaattcc accactttgg agagggttgc ctttccagct
     1981 tgtaaaaaac cagcttccgc gtgaagtagt gtagttgaga tctatattgt aaaccttgtc
     2041 tttcggacct tcgtcaattt gttttttaag tccgagaatt acatcatgat aacgtagttg
     2101 aagtattgta taaacttttt tacctgtttc cttttctact aactctaagt catcaaggtt
     2161 ttttggattt agaaccattg gtttctcagt tatcacattg gctccaattt ttaatccgta
     2221 gcgaatgtgt gcatcgtgca gataatctgg tgtgcagacg gagagatagt ctattccttg
     2281 tccggcactt tgaagttttg caagatgcct gtcaaaaagc tcaaactgat tgaaatatga
     2341 gcattcaggg aaataactgt caagaaggcc aacgctgtca aaaggatcaa cgcatgagac
     2401 cagggtattc ccggaatttt taattgcctt caggtgtctt ggggcaatat aacctgcgac
     2461 acccattaat gcgaattttt tttctgtttc caagttatta tattttttct tttttatttc
     2521 cagccaaaag gatgtggagt ctgttgaaga cgtgcaaatg gatgatagtc acctttcagt
     2581 ccgattggtg ttgcatttct gatgtcatgc actatctgta tacatttacg ggcatcttca
     2641 agaccaaatc ccttgccttc gaggatgtgc ctgtatgagg cggtatgaag ctctgtaaat
     2701 ccttctgaaa attccacttc ttctccttcc atggttaggg atctgtatgt tcttttgcct
     2761 gactctctta tctcgttagg aagtgtgttg acgtttatac ttagaaagta tcttacttta
     2821 gccttgtcaa attcaagata tcctgccacg cggtcatggg atgatatatg tacaatattt
     2881 tgcttgacat ctccgaaaat ccagccaagc atgtcgtaaa agtgaactcc tatatttgtg
     2941 gctactccgc cgctcttgcg cggatctcct ttccagctgg tgtagtacca gttgcctctt
     3001 gaagtaatat atgtaagatc aacatcatat attttgtcct ttggtccctc gtcaactctt
     3061 tttttaagtg caatgatagc gtcgtgcaac ctgagttgaa gtatattgta tattttgtgg
     3121 cctgtctctt tttctgcaaa ttcgagtgca tcaatattcc atggattgag tacaataggc
     3181 ttctcacaga taacatcagc tccttctctt aatccaaatc ttatatgagc gtcatgcaga
     3241 taatttggtg tacatattga cacataatct aatttacctt ttggataatc tttttcgagg
     3301 tctccggtgt ttgaaaaggc tctctttttg gaaagatgtc tttcaaaaag ctcatactgt
     3361 ttgaagaatg cgcattttgg aaaataagaa tcaactattc caacgctgtc gtttatgtcg
     3421 taggctgcta tcaagttgtt tccagtatcc ttgatggctt tcatgtgccg aggagcgaca
     3481 tatccagctg cgccaatcaa tgcaaaattt ttcatcattt tttctataaa ctgcggcaaa
     3541 gataattaaa aatagttatg attgtctgta catctcatca tagtatcttc tgtaatcgcc
     3601 ggaggtgacg ctgtcaagcc attcctgatt gcttagatac cagcgtacag tcttagtaag
     3661 tccatcttca aactgtacgg aagggctcca gccaagttct gtttgaagtt ttgtggaatc
     3721 tattgcatat cttagatcat gtcctgctct gtctgttacg taagttatca atttttcact
     3781 gcttccatcc tctcttccca gttctctatc tactatcttt actataagtt tcacaaggtc
     3841 gatgtttctc cattcgttga atcctcctat attataagtt tctccaacct ttcctttgtg
     3901 aaaaataaga tcgattgcag ctgcatgatc ctcgacatac agccaatctc tgatgttctc
     3961 acccttacca taaaccggaa ggggctgatt gtgcctgata ttgtttataa gaagaggaat
     4021 gagtttctcg gggaattgat aacttccgta gttgttggaa cagtttgaaa taattatcgg
     4081 aagcccatat gtgtcatgat acgctcttac aaaatggtct gaagcagcct tggaagcgga
     4141 gtagggagag tggggttcat acctgctggt ctctttaaac aaagatatat tgtcattcag
     4201 tgaaccataa acttcgtctg ttgagacatg gtaaaaaagt ttattttcga aattattttc
     4261 ccatgccagt ttggctgcct gaagcagtga gagtgtaccc attacattgg tttgggcaaa
     4321 tgaaaaagga tccttgattg atctgtctac atgtgattct gctgccaggt gcatcactcc
     4381 gtcaatattc atctctgaaa aaatgcttct tactaattca aagtcgcaaa tatcaccttt
     4441 tatgaatgtg tagttttttt tgttttctat atccttaagg tttgatagat tacctgcata
     4501 agtcagttta tcaaggttta ctatgctata ttcaggatat ttgttaacca ttagacggat
     4561 caagtgcgag ccaataaatc cggctcctcc ggtaatcagt atatgtctca tagtcttgtt
     4621 tgtaatatta ttttcataca tttttcaaga gaatctctcc aatgtggaat atttatgcca
     4681 taaaccttct taatgagagt tttgtcaagc acagaaaagg caggccttgt tgctttggaa
     4741 ggaaactctt cacttcttac gggatggatg gggagtgtat ttgaggtaaa ttttataatt
     4801 tcgaccgcaa aatcatacca agaacaaacg ccttcatttg agaaattata tatatggttt
     4861 tcagcatttt taatatagaa aagctcatct ttgtgagctg atatttctga aattgcaagg
     4921 gcgagatctg ctgcaaaggt tggggtgcct gtctgatcaa atacaacttt cagttccttt
     4981 ttttcttttg cgagacgaac gattgttttt acgaagttat tgccataaat agaatataac
     5041 caagatgttc gaagaatcag atatctgcat cctgaatttt caatagcctt ctctcctgca
     5101 agttttgtct ttccgtatac agagaccggg gcagtgatgt ctgattcttt atagggtttt
     5161 ggtcctttgc cattaaagac gtaatctgta gagatgtgaa gcaggagtgc attgttctct
     5221 ctggcagctt ctgcaagaaa tccgggggct tctgcattta attggtatgc agcggccggc
     5281 tcgtcttctg ctttgtctac tgcagtataa gcagcgcagt ttattatcag atctatattg
     5341 ttctttttaa taaattgaat tacggactct ttctgacaaa tatcgagttc ttcgacatct
     5401 gtataataaa cagtaccggg caacttgttt tcgattgccc gtaattcatt acccagttgg
     5461 ccgtttgagc cagtaactaa agtccctgtc attggttatt gtctatcatc tttatattca
     5521 aaaaggtaag catcttttag aagtggatgt ttcttatctt tgtcgctgag aataacgtct
     5581 ttggcaggta ttccccattt aatagcaagt gacggatcat tccagagaat tgctctttca
     5641 tgacttggcg aatagatgtt atcggtctta tattgaacaa ttgtattatc ttcaagcact
     5701 gaaaatccat gggcaaaacc ttttggaata aaaagctgtc gtttgttttc tcccgagagt
     5761 tcgacggcca tatatctgcc aaatgttgga gaacctttgc gaatgtctac tgctatatcc
     5821 attatcctgc cggtaactac tctgattagc tttgactggg caaatggttc tgcctgatag
     5881 tgtaagccgc gtaatactcc gtattttgaa caggactcgt tttcctgaac aaaagatatt
     5941 ttagaaatct ccctttcaaa ttctgacaat agaaaggact ccatgaaata tcctctccca
     6001 tctccatgaa caattggttc tatgatatat acatcgggta ttgctgttct aataaaattc
     6061 attttctctt ttttgtaaga ttaataaggt attgtccata ttggtttttt atcattggtc
     6121 gagcaatttc aataagcttc ttgtcgttaa tccagccatt tttgtatgcg atctcttcga
     6181 gacatgcaat tttaagacct tgtcgtttct caattacttc gacgaatgtt gaggcgtctg
     6241 atagcgagtc gtgagtacca gtatcgagcc atgcaaatcc acgaccgagc agttgaacat
     6301 ttaatttttt ctctttgaga aattcctggt tgacggttgt aatttccagt tctcctctgt
     6361 cagatggttt tatggttttt gcaaccttta ctactttgtt gggatagaaa tagaggccaa
     6421 ctactgcata attgcttttt ggatttgtgg gtttctcttc gatactgagc actttaccgg
     6481 ttttgtcaaa ttcagcaaca ccatatcttt ccggatcgtt gacccagtaa ccgaataccg
     6541 tagcttctga agactcttca gcacttttga cagcattttt taacattctt gaaaaaaatt
     6601 ggccgtaaaa gatattatca ccaagaacga gacatgcaga atcctgtcca atgaattttt
     6661 caccaattat gaaggcttgg gcgagtccgt ccggtgaagg ttgttctgcg tattcaaaat
     6721 gaacgccaaa atcgctaccg tctccgagca gtctgcaaaa accgggaaga tcgtccggtg
     6781 ttgagatgat taatatttct cttattccgg caagcattag taccgaaata gggtaataga
     6841 ccatcggttt gtcatatatt ggcagcaact gtttggaaac tcccttagta atagggtaaa
     6901 gccttgttcc agaacctccg gccaggacta ttcctttcat gtcaaattgt attatttatg
     6961 tctaaataga ccccgttttt ttgtttttgg taattgctcc tcattttgtt gaaatatttc
     7021 aggagaaaaa cattcatcag gtttgtattt cagcgcattg taaataaatc cccaatcggg
     7081 tactcctcca aaatttctgt cgtcaatata catgtctgcg atcaattttc ttggtgaatc
     7141 attacctgtg caatccgggt gattttcgtt gacagcatag aattcaagac ctctcgattt
     7201 gcagtattcg acagcctctt caaga
//
LOCUS       DAOTQL010000091         7161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8750_length_7161_cov_4.995356, whole genome shotgun sequence.
ACCESSION   DAOTQL010000091 DAOTQL010000000
VERSION     DAOTQL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8750_length_7161_cov_4.995356"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1460
                     /locus_tag="P5293_06075"
     CDS             141..1460
                     /locus_tag="P5293_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR49505.1"
                     /translation="MKITNILPVLFLLYVPIQLPAQKSWSLEECIKYAWDNNLMVKQK
                     EISVNQSNNNVFQSKMDYLPSMNASVSHSMNWGRSVNMQDLQIVENKLSQSMSASLNA
                     SLTLFEGLQKTNNLKSKEVLLQISELDVKRTKNQLALQISKNYLEVMLAKEILKSADQ
                     SLASVEEQVKRTEKLVDAGSVAYSSLLEIQSQLATERVQVVSAKNQVNSALLTLRQLL
                     DLSYDTHFDILTPDLGVMISEFDGESVDSLYELSRDLPEIQSALLNQKNSKLQLSVAK
                     GASYPSVSFSAGYGTYYSDQREETMMDQFNENRNPSISFGLRIPIFNNWQTNTAIRNA
                     HLNVKSAEIDLKTAEQTLFKEIQQANNDAIAYFEKYKASIENVKSMEESFRYVQQKFE
                     IGVLNATDYTVAKTNLFKAMSDLLQAKYQFVFQLKVLDYYKGIPITL"
     gene            1475..2746
                     /locus_tag="P5293_06080"
     CDS             1475..2746
                     /locus_tag="P5293_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR49506.1"
                     /translation="MKKNKQNRILIIAALIILILIVVGMAGKKESVTVTAEKVKLNTI
                     TEVIPANGKIQPVTEVKISPDVSGEIIELNVKEGDKVKRGDLIIKIKQDVYISMKERA
                     EASLNAAKAQLDQQLAQFGQIEANYIRNKKLYEQKAISEKEYEDALYQYEIAKEQIKG
                     ARFNVNSYDAALKEANENLYKTSIYAPMDGIISKLSVEKGERVVGTSQMAGTEMLRIA
                     NFNEMEVLVDVNENDIIRIKDRDTAKIEVDAYPNRKFTGIVTQIANSAKNIGSAVDQV
                     TNFEVKVLILPESYSDLMVDGKNPFRPGMSASVSIETATKHNVLTIPLQSITTRTDLL
                     SDSLKKSLSVNESVENVFIVKNDTLQVRRITTGIQDLNNIEVVTGLEEGEMVVTGPFS
                     GISKTFKSGIKVTIEDPNKKKQGSEKSKSVS"
     gene            2750..3445
                     /gene="tsaB"
                     /locus_tag="P5293_06085"
     CDS             2750..3445
                     /gene="tsaB"
                     /locus_tag="P5293_06085"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRR49507.1"
                     /translation="MSPLLLLIETSTDICSVALTRGEFLLCSHSSNEPKSHARIIAPL
                     IKDILEEYSLSTTDCDAIAVSEGPGSYTGLRVGASIAKGICYGTDIPLIAINSLEPIV
                     RLVAAKIVDYPENTLIVPMIDARRMEVYTSIFNMKCEAVSQTEAMIINEGSFLNELQS
                     GIVVFTGDGALKCKSIITHPNAVFIESKTDASGMILPALKKFYEGKFEDVAYFEPYYL
                     KDFVAGVQKKRLF"
     gene            complement(3450..4592)
                     /locus_tag="P5293_06090"
     CDS             complement(3450..4592)
                     /locus_tag="P5293_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRR49508.1"
                     /translation="MRRLIAFLVAGVLAISCGSDKSIIKGSIKGLNDSKVVLKHLAFN
                     TQEIVDTIPVEEGKIFYRLKTHSDSPEFYYLYYNSNKIASFVILPGNEIKFSTDTLGN
                     KVISEGSEETLLLQEIEKNAAKVAFKFDSLVKVKSEYDSVGDTASSKSVDYQLGSLYV
                     KQKQGAIKHIYTYPNSITNVPLLYQQFPNGLPIFADSRDVMLFKRVCDSLSNKYPKST
                     FVANLKDEIAKRDKILALNDKIEEANISGHPDISLPDINSRKRSLSEFEGNVILLSFW
                     SSQDVNQKMMNQELMKLYRKYNDKGFEIYQVSVDMDKTAWARAVTDQQLPWISVCDGK
                     GPNSSGVVSYNVNKVPSIFLISKDGSIVAKDIFGEELEQKVSSLLR"
     gene            complement(4612..5007)
                     /locus_tag="P5293_06095"
     CDS             complement(4612..5007)
                     /locus_tag="P5293_06095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRR49509.1"
                     /translation="MSTVFTRIVNGEIPSYKVAENDKFYAFLDINPVTKGHTLVIPKE
                     EIDYIFDMDSKDLSDLFVFAQTVAKGIKKVIPCKRIGVAVLGLEVPHAHIHLIPLVSE
                     GDMDFKKTKLKLEHEEMVAIASAIAAAIK"
     gene            complement(5010..5483)
                     /gene="greA"
                     /locus_tag="P5293_06100"
     CDS             complement(5010..5483)
                     /gene="greA"
                     /locus_tag="P5293_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRR49510.1"
                     /translation="MSKIHYLTSEGLEKLKEEYRHLVSVERPAITQAIAEARDKGDLS
                     ENAEYDAAKDAQGLLELRIAKLEDMIANARIIDKSKINTKHVQMLNKVKLKNLNNNSI
                     VEYTLVSESEANLSEGKLAAATPIGKALIGRSKGDVVEVKVPSGVIKFEILDISI"
     gene            complement(5590..6504)
                     /locus_tag="P5293_06105"
     CDS             complement(5590..6504)
                     /locus_tag="P5293_06105"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRR49511.1"
                     /translation="MVIGTTGFFDGVHRGHQAVISKVYSLAKECGGRSVVVTFWPHPR
                     AVLQQDAAKFRLLTSLDEKRKLIKSLGIDEVVVLPFNKEFAKQSTEEFFKTYLVDMLG
                     IETLVVGYDHHVGNDINQTQEEMFRIARMLGITPVRIEECKTDAVNSKISSTKIREFI
                     RQGMIEEANILLGYRYGLEGVVVEGQRLGRKLGFPTANIKLYEPLKLIPADGVYIVWV
                     EYSGKTYRGITNIGTRPTIGENNEITIETHLLDFNEDIYGLSIKIEFAARLRNEKRFE
                     SIEQLKEQIGKDKENCYKLMPEINVKLR"
     gene            complement(6495..>7161)
                     /locus_tag="P5293_06110"
     CDS             complement(6495..>7161)
                     /locus_tag="P5293_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRR49512.1"
                     /translation="WALPLDTTYDDQSLQDELDAANIYNILESEIIPLFYKREKKNEL
                     PIEWIQCIKNTIAKVASNFTTHRMIEDYQHKFYIPLYERNNKIIENDFALAKELSFWK
                     KRVRREWPLIEVKSYIKPESSKEEISKGNEYYAEVELIIGDLKPEEIGVEMVFASKDK
                     NNRMRFLKKYDFDLVSFEDGIVKYKCIMMPETAGVYNFAGRIYAKHPDLPHRQDFDLV
                     KWL"
BASE COUNT         2284 a         1378 c         1245 g         2254 t
ORIGIN      
        1 ctttggaagc tttttaaagt aatcagtaat aatatttact ccatgagatc taatcctgta
       61 ttattgtacc agtgacaaaa ataatcattt aaaaataatt gaccgggtgt ttgttaagta
      121 aaaagtaata tatttgtcat atgaaaatca ctaacatact cccggtttta tttcttcttt
      181 acgtcccgat tcaactcccg gcacaaaaga gttggagttt ggaagagtgc ataaaatatg
      241 catgggacaa taatcttatg gttaaacaaa aagagatatc tgtcaaccag agcaataata
      301 atgttttcca gtcaaaaatg gactatcttc catctatgaa tgcctctgtt tcacacagca
      361 tgaactgggg acgttctgta aatatgcaag atttgcagat tgttgagaat aaactatccc
      421 aaagtatgag tgcttctctt aatgcatcgt taactctgtt tgaaggttta cagaaaacca
      481 ataatctaaa aagcaaggag gttctgttgc aaatttcaga gttggatgtt aaaagaacca
      541 agaatcagct tgcattgcaa atctccaaaa attatcttga ggtaatgctt gcaaaggaaa
      601 ttcttaaatc agctgatcaa agtcttgcta gcgtagaaga acaggttaaa cgtactgaga
      661 aactggttga tgccggaagt gttgcatata gctctcttct cgagatacag tctcaattgg
      721 cgactgaaag agtacaggtt gtctcagcaa aaaatcaggt taattctgct ttactcaccc
      781 tcagacaact tctcgacttg tcatacgaca cacattttga tattctgact ccggatttgg
      841 gtgtgatgat ttcggagttc gatggggaat ctgttgattc tttgtatgaa ttatccagag
      901 atttacctga aatacaaagt gctcttttaa atcagaaaaa ttcaaaactg caactatcgg
      961 tagccaaggg tgcaagttat ccttctgttt ctttctcagc cggctatggt acttactaca
     1021 gtgatcagag agaggagacc atgatggatc agttcaatga aaatcgcaat ccttctatct
     1081 ctttcggact tagaattcct attttcaaca actggcagac aaatactgcc atacgaaatg
     1141 ctcatctgaa tgtcaagagc gcagaaattg atcttaaaac tgcggaacaa actctcttta
     1201 aggagattca acaagccaat aatgatgcaa ttgcttattt tgaaaaatac aaagcctcca
     1261 ttgaaaatgt caaatctatg gaagaatctt tcagatatgt ccagcaaaag tttgagatag
     1321 gtgtgctgaa tgcaactgac tacacagttg ccaagactaa tcttttcaag gctatgtcag
     1381 atcttttaca ggcaaaatat cagtttgttt tccagcttaa agttctcgat tattacaaag
     1441 gcattcctat tactctttaa aacattctat tctaatgaaa aaaaacaaac aaaaccggat
     1501 tcttataatt gctgctttaa tcattctgat tctgatcgtt gtcggaatgg ccggtaaaaa
     1561 ggaaagtgtt acagttacag ccgagaaggt taagcttaac actataactg aagttattcc
     1621 cgcaaacgga aagattcaac cggtaacaga ggtaaagata agccctgacg tttcaggtga
     1681 aatcattgag ttaaatgtta aagaaggaga taaagttaaa aggggagacc ttattattaa
     1741 aattaaacag gacgtttaca tttccatgaa agagagggca gaagcttcat taaatgctgc
     1801 caaagcccag ctcgatcagc aacttgccca attcggtcag attgaggcaa attatataag
     1861 aaacaaaaag ctatatgaac agaaagccat ttcagaaaaa gaatatgaag atgctcttta
     1921 ccagtacgaa atagccaagg aacaaataaa aggagcccgg tttaatgtta acagctatga
     1981 cgcagcactt aaagaggcaa atgaaaatct ttacaagacc agtatttatg ctcctatgga
     2041 cggcattatt tcaaaactta gcgtagaaaa aggtgaaaga gttgtaggca caagccaaat
     2101 ggccggcaca gaaatgctca gaatagctaa tttcaatgaa atggaagttt tggttgatgt
     2161 caatgaaaat gatattatca gaataaagga tagagatact gccaagatag aagtggatgc
     2221 ttatccaaac agaaagttta caggtattgt gacacaaata gcaaattcgg caaaaaatat
     2281 tggctcagca gtcgaccaag tcacaaattt tgaggttaaa gtgttgattt tacctgaatc
     2341 atattcagat cttatggttg acgggaaaaa tcctttcaga ccaggaatgt ctgcttctgt
     2401 ttcaattgag acagctacca aacataatgt actgacaatt cctttacagt ctattacaac
     2461 aaggactgat cttctttcag attctcttaa gaaaagctta agcgtcaatg aatctgtcga
     2521 aaatgttttt attgttaaaa atgacacact tcaagtgaga agaattacaa cgggcatcca
     2581 agaccttaac aatattgaag ttgtgactgg acttgaagaa ggagagatgg ttgttaccgg
     2641 acctttttcc ggaattagca agacctttaa aagcggcata aaagtgacta tagaagaccc
     2701 aaataagaag aaacaaggat cagaaaaaag taaatccgtc agctaaatta tgagtccttt
     2761 gcttttgctt attgagacaa gtactgatat ctgctcggtt gctctgacaa gaggagaatt
     2821 tctattgtgt tcccactcta gcaatgaacc aaaatctcat gcaaggataa ttgctccctt
     2881 gattaaggat attcttgaag agtattctct ttcaacaacc gactgtgatg ccatcgctgt
     2941 aagtgagggt ccgggttctt atacaggatt aagagttggc gcctccatag ctaagggaat
     3001 ttgttatgga acagatatac ctttgatagc tataaactct cttgaaccta ttgttcgcct
     3061 tgtggctgca aagattgttg attatcctga aaatacttta atagtaccga tgattgatgc
     3121 gagaagaatg gaggtttata cttcaatatt taacatgaag tgtgaagctg tttcgcaaac
     3181 tgaagctatg attattaatg agggaagttt tctgaatgag cttcaaagcg ggattgtagt
     3241 atttaccgga gatggagcat taaaatgtaa aagtataata actcatccaa atgcagtatt
     3301 tattgaaagt aagacagatg cttctggaat gatcttacct gcactcaaaa aattctatga
     3361 gggaaaattt gaagatgttg catactttga accttactat ctgaaagatt ttgtggcagg
     3421 agtgcaaaaa aaacgattgt tttaaaggat tatctcagca aactcgacac tttttgttca
     3481 agttcttccc cgaaaatatc ctttgcaaca atagacccat ctttagaaat caaaaaaatt
     3541 gatggtacct tattcacatt gtaagagaca acaccactcg aattgggacc tttgccatca
     3601 catacactta tccatggcaa ttgctggtct gtaactgctc ttgcccatgc tgtcttgtcc
     3661 atatcaaccg acacctgata tatctcaaaa cccttgtcgt tatattttct gtaaagtttc
     3721 atcaactcct ggttcatcat cttttgattg acatcttgtg aactccagaa agagagtagt
     3781 atcacattac cttcaaattc tgagagagat cttttccttg aattgatatc aggcaaagaa
     3841 atgtcagggt gaccggaaat attcgcttct tcaattttat cgttcaatgc cagaattttg
     3901 tctctcttgg ctatctcatc cttaagattt gccacaaatg tagattttgg atatttatta
     3961 gataatgagt cgcataccct cttaaaaagc attacatctc ttgagtcagc aaaaataggc
     4021 aggccattag gaaattgctg atatagcaaa ggcacattgg tgattgaatt gggataagta
     4081 tagatatgct ttatagcccc ctgcttttgt tttacataca aagagccaag ctggtaatcg
     4141 acagatttgc tcgaagcagt atctccgacg ctgtcatatt cagacttaac tttgaccagt
     4201 gagtcaaatt tgaaagcaac tttagcagca ttcttctcaa tctcctgcaa aagcaatgtc
     4261 tcctcagatc cttcggatat taccttattc ccgagtgtgt ctgtagaaaa cttaatttcg
     4321 tttccgggaa gaataacaaa agaggcaatt ttgtttgagt tatagtataa ataataaaac
     4381 tccggagaat cactgtgcgt ttttaatcta taaaatattt taccctcttc cacaggaatt
     4441 gtatctacaa tctcctgagt gttaaatgca agatgtttaa gtactacctt gctgtcattg
     4501 aggcccttga tagaaccttt aataatcgat ttgtcagatc cgcaagatat tgccagcaca
     4561 cctgctacta aaaatgctat taatcttctc atagccaaaa gattttgaat actatttaat
     4621 cgctgctgca atagcagagg cgatagcgac catctcttca tgctcgagct tcagttttgt
     4681 ttttttaaaa tccatgtcac cttcgctaac aagaggaata agatgaatat gcgcatgagg
     4741 tacttccaaa cccaagacag caactccgat tcttttgcaa ggaatgacct ttttaatgcc
     4801 tttggcaaca gtctgggcaa atacaaatag atctgacaaa tccttactgt ccatatcaaa
     4861 aatgtaatct atttcttctt ttggtatgac aagggtatgg ccttttgtaa cagggttaat
     4921 atcaagaaat gcataaaact tgtcattttc tgctacctta taagagggaa tttccccatt
     4981 gactattctg gtaaaaactg ttgacatttt taaattgata tatccaaaat ttcaaattta
     5041 atcacacctg atggtacctt aacctcaacg acatcacctt tagatctgcc gataagagct
     5101 ttgccaatag gagtcgctgc agccagttta ccttcactaa ggttggcctc gctctctgaa
     5161 acgagagtgt attcgacaat agaattatta ttgagatttt tcagcttaac tttattcagc
     5221 atctgaacat gctttgtatt tatcttggac ttatctataa tcctggcatt ggctatcata
     5281 tcctcaagct ttgctatcct aagttcaagg agaccctgag catccttggc agcgtcatac
     5341 tcagcatttt cagacaagtc tcccttgtct ctggcctctg caatagcctg agtaattgcc
     5401 ggtctctcca cactgaccaa atgacgatat tcttctttga gcttttcaag cccttccgaa
     5461 gtcaagtaat gaatctttga cataatttta aaaaatcaaa aagaatcctg cgtaaacagg
     5521 actctttaat gcaaagataa taaaatttat ttataaaact aatattctat aattatatat
     5581 cttcttaaat tatcttaatt taacattaat ttcaggcatg agtttgtaac aattttcctt
     5641 gtctttacca atctgctctt tcaactgttc tatcgattca aatcgctttt cattcctcaa
     5701 tcttgcagca aattcaatct taatagagag cccataaata tcttcgttaa aatcgagcaa
     5761 gtgagtctcg atagttattt cgttgttttc tccgatagta ggtcttgtac cgatgttagt
     5821 aatgcctctg tatgttttcc cagaatactc gacccaaaca atataaactc cgtcagcagg
     5881 aattagcttt aagggttcgt acaatttaat attggcagtc ggaaaaccca atttgcgccc
     5941 taatctctgt ccttcaacca caactccttc aagtccatat ctatatccaa gtaaaatatt
     6001 ggcttcttca atcattcctt gtctgataaa ttcccttatt ttagtagaac ttatttttga
     6061 attaactgca tcagttttac actcctctat ccttacaggg gttattccca acattcttgc
     6121 aattctgaac atctcttcct gagtctgatt aatatcattt cccacatggt ggtcgtaacc
     6181 tactaccaaa gtctctattc caagcatgtc cacaagatat gtcttgaaaa attcttcagt
     6241 actctgtttt gcaaactctt tgttaaaagg gaggacaact acttcatcta tacccaaact
     6301 ttttatcagt ttacgttttt catccagtga tgtaagcaac ctgaacttag cagcatcttg
     6361 ctgtaaaaca gctctgggat gaggccagaa ggtcacaaca acgcttcgtc caccacactc
     6421 ttttgccaac gaatatactt tgcttattac agcttggtgt cctctgtgaa caccatcaaa
     6481 aaatcctgta gtccctataa ccattttact aaatcaaaat cctgtctgtg aggtaaatca
     6541 gggtgctttg cataaatacg tccagcaaaa ttataaactc cggcagtttc aggcatcatt
     6601 atacacttat atttgacaat tccatcctca aaactcacaa gatcaaaatc atattttttt
     6661 aagaatctca tacggttatt tttgtctttt gaagcaaata ccatctctac acctatctct
     6721 tcgggcttta agtctccgat tatcaactca acttcggcat agtattcatt accttttgat
     6781 atctcctctt ttgagctttc cggttttata tagcttttga cttcaatcaa aggccattct
     6841 cttcttaccc tctttttcca aaaagatagc tcttttgcta aagcaaaatc attctcaata
     6901 attttattat ttctttcata aagaggaata taaaatttat gttggtagtc ctcaatcatt
     6961 ctgtgggttg taaaattaga ggccactttg gctatcgtgt tctttataca ctgaatccat
     7021 tcaatcggaa gctcattttt cttttctctt ttatagaaca aagggattat ttcagattca
     7081 agtatattat agatattggc agcgtcaagc tcatcctgaa gagactggtc gtcataagta
     7141 gtgtcaagag gcagtgccca t
//
LOCUS       DAOTQL010000092         7160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8754_length_7160_cov_4.050106, whole genome shotgun sequence.
ACCESSION   DAOTQL010000092 DAOTQL010000000
VERSION     DAOTQL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8754_length_7160_cov_4.050106"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="P5293_06115"
     CDS             <1..>1169
                     /locus_tag="P5293_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49513.1"
                     /translation="GIRINQFGASYGTPPIVNSYGLYISDGSETTVSGVNYNFYSAGA
                     TALNYFEGILTVAGGTNFNKTSDDDVGYITTYTKSRGAAGQDGDDIGTISFKSYNDAG
                     TPELIEYARILSDIADSSDASERGRVTFTVRCMGDTTRDPLVICQPAISGATGLVTIN
                     AVGFINNNAMYINAAGAANGIWADASQCFCAKPSQSGCIGFIADDRTASGGTALQIIA
                     VNSSGNVYPMIEYRGYTNLSGNFIHFDVAYENYDVNFTGNFILWEKYTTEYFKIDQYV
                     DFSIWRRTADAVGHTTTFIKTRAAAGQDGDDIATFSFKSYNDAGTPELIEYARIVAEI
                     NDASDGTEDGQLTFYSMDAGTLIDTFRIFSGRSLMYRDDIALGGGSGYTQLGIDL"
     assembly_gap    1170..1179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1180..2023
                     /locus_tag="P5293_06120"
     CDS             <1180..2023
                     /locus_tag="P5293_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49514.1"
                     /translation="IAATSSGNEGFTGFNVQASLDTAVAHNCSVIGASVTANNMSSVV
                     QTDVTSIYLSALDVSEATGGCTKLVGAKLFLMAMGEGAVDDAIGIEVNPVILPAGSVD
                     TVSSIKIVAPTLTGTVTDLYGIYVEDQTATVSGVNANIYSAGITSLNVFEGTIRSYRS
                     VNDATANILEFRKTRGSAAGVIGDEIGVISFWSNNSASSLVEYVNLTAKIADETDNEE
                     CGLITYSAMISGSGYEGNLPMVYTKETGGDLTDAFEGMIVINTNDNTLKMYAKGAWRQ
                     LATW"
     gene            2070..2468
                     /locus_tag="P5293_06125"
     CDS             2070..2468
                     /locus_tag="P5293_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49515.1"
                     /translation="MIIKNLVLEALTRTDILSKLLNSKDLPVRSSFQLGKLVKLLSDA
                     IKDYTDAKQKLLEKYAERDEAGKLKVENNQYIVSSENILQFNSDLTELLEIDIDLPMD
                     KVKINLDNIPTGLISPVDFPFLENFIEFEE"
     gene            2465..2770
                     /locus_tag="P5293_06130"
     CDS             2465..2770
                     /locus_tag="P5293_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49516.1"
                     /translation="MSQSYITVNQNETRAIELLIRDQNDDPVYPDSAYARVVDAYTDI
                     PVVSEMVAMVDGNKIYFLLTPVVTAEPGMYDVIWRIVKTETSIYTFYHKTRVTVNEL"
     gene            3035..>7160
                     /locus_tag="P5293_06135"
     CDS             3035..>7160
                     /locus_tag="P5293_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49517.1"
                     /translation="MRYHFIGRSRDSYGNTLQGVDIYVYLAGTTTAAKVYTAMTGGTA
                     ISIAPQVESDANGLFSFYVDDGDYDTTQKFDIVVGATRYENIDIYRRGIDGSSGTSGT
                     SGTSGETGPIGPQGTGGSSGTSGTSGSSGTSGETIFPGGYLHTQSLASSAWSITHNLG
                     EKYVVVEVSDEDDLSVIPSEILYVDANNLVIIFPSDVAGQAKIVAGTGTSGTSGEDGT
                     SGTSGSSGTSGSSGTSGIDGTSGTSGTSGETTFPGGYLHTQSVPSSAWSVTHNLGEKY
                     VVVEVADDDDLYIIPSDILFVDSNNLLITFSEAISGHARIIAGTGTSGISAIGGAFTY
                     TQSVPSTAWSVTHGLGVQPIAVAVMNGDGYGIEPGDILYVDDNNVILTFDLPISGSAT
                     FVSGGGAVGTSGTSGISGTSGTSGTSGSSGSSGSSGSSGTSGTSGTSGSSGTAGTSGV
                     DGVFGGPSALYKFNNTSTDVDVDPGLGKILFNNTSFSSVTRINVNHYDDGGAYLYNWL
                     DIFTLTNNNSLIKIFNETDPTKFVIYRITGLTNRSGYKTFWVSYLSSNGTIFNNGTNI
                     VLTVSLIGSSGSSGTSGTSGTAGTSGTSGSSGTSGTSGSSGTSGTSGTSGTSGTAGTS
                     GSSGTSGSSGSSGTSGSSGTSGSSGSSGSSGTSGWGTSGTSGTSGIDGTSGISGTSGT
                     SGSSGTSGTSGTSGSSGTSGTSGTSGTSGTSGTSGTSGIDGTSGTSGIDGTSGSSGTS
                     GTSGFSGIDGTSGTSGEDGTSGTSGTSGSSGTSGFSAAWGVPYRFDTDTTDTDPNIGH
                     LKFNSTIFSSVTRIYIDLMDRYGQILTPWIDLMDDSTSLWSKGYLKIWRDHDETDYAF
                     FEINGTTDIDPQYRKLHVGLIAASSTEVSGAFLEDDDLQIGFVPTGDAGTSGTSGLAG
                     LGANAHLYWFDNDITDSDPPSGHLKLNNADPTLATYIYVDVLNRFGTDVEAWLASLVD
                     SNAVRIMNQNSVEIFHDYLLLSAPTSGAGYYKLNVGYLVGNGTLNDEDEIVLGIGNRG
                     QTGTSGTSGTSGTSGTSGRGYLHVQSTPASTWSINHILNCRYCVVEVTDENAYSIIPD
                     TIRFDDENNLTITFSEAISGRAFISSGGGLAVGELAYTFSTPVTAWNITHNLGNKYVN
                     VQCVDGDDYYIEPQSIQFIDENNIEITFSTPVSGVCSIVGGVGTIGTYGSSGTSGSSG
                     TSGEGTPTGVILPYIGTTAPAGWLLSDGKTIGNLSSGATGRANADTQTLFELLWNSTT
                     NTELVIQDSAGSPTTRGVTAALDFTANKRMPLPDLRGRIPLGKDNMGGSSANVVTAPA
                     ADLIAGEGGAENHTLTEAEMPSHRHYTPTITRTVYDFTGGDQGYGQDTPTGSGPN"
BASE COUNT         2129 a         1272 c         1650 g         2099 t
ORIGIN      
        1 atggtatcag aatcaatcaa tttggagcat catacggaac gccacccatt gtcaacagtt
       61 atggtttgta tatttcggat ggctctgaaa ctacggtgag tggggtaaac tacaattttt
      121 attctgccgg agcaacagca ttaaattatt ttgaaggtat tctaacggtt gccggtggaa
      181 cgaatttcaa caagacttcg gatgatgatg tcggttacat aacgacttac acaaagtccc
      241 gtggtgccgc cggtcaggat ggagatgata tcggtactat ctcattcaaa tcctacaacg
      301 atgccgggac gccggagttg attgaatatg ctcggattct atcggatatt gctgactcaa
      361 gtgatgcgag tgaaagagga agagttacat ttactgttcg ttgtatggga gataccacaa
      421 gagatcctct tgttatttgc caaccagcga tctcaggtgc aaccggatta gtcactataa
      481 acgcagtagg ttttattaat aataatgcta tgtatataaa cgctgctggg gccgcgaatg
      541 ggatatgggc tgatgcgtcc cagtgtttct gtgccaagcc ttcacagagc ggctgtattg
      601 ggtttatcgc tgatgacaga acagctagtg gtggaacagc attacaaata atagctgtaa
      661 attcatctgg aaatgtctat ccaatgattg aatatagagg ctatactaat ctctcaggaa
      721 actttatcca ttttgatgta gcttatgaaa actacgatgt taattttaca ggtaacttta
      781 tcctatggga aaaatatacc actgaatatt ttaagattga tcaatatgta gatttctcta
      841 tctggcgcag aactgcagac gccgttggtc acactacaac attcataaag actcgcgcag
      901 cagcaggtca ggacggtgat gacattgcca ccttctcatt caagtcatac aacgatgccg
      961 gaacaccaga gctgattgaa tatgctcgga ttgtggctga gataaatgat gcatcagacg
     1021 gaacggaaga tggacaactt actttttaca gtatggacgc aggtacatta atagatactt
     1081 tccgtatatt ctctggtcgt tcactaatgt acagggatga tatcgctctt ggtggtggtt
     1141 caggatatac acaattaggt atagatttan nnnnnnnnna atcgcagcca cttcctctgg
     1201 taatgagggt ttcaccggct ttaatgtaca ggcctcttta gatacggctg tcgctcataa
     1261 ctgttctgtc atcggcgcct ctgttactgc aaataatatg tcttcagttg tccagacaga
     1321 tgttacttcc atttatctga gtgctttgga tgttagtgaa gcgacaggag ggtgtaccaa
     1381 gcttgttggt gcgaaactat ttcttatggc aatgggagag ggggccgtag atgatgctat
     1441 cggcatagag gtgaatcctg tcattttacc tgctggttcg gttgataccg ttagttctat
     1501 taagattgta gcgcccacgc ttaccggaac agtaacggat ttatacggca tatatgttga
     1561 agaccaaact gcaaccgttt caggagtaaa tgctaatatc tattccgctg gtataacatc
     1621 tcttaatgtg tttgaaggta ctattcgttc atataggtct gtgaatgatg caactgcaaa
     1681 cattcttgag ttcagaaaaa ccagaggttc tgcagccggt gtcatcggtg atgaaattgg
     1741 tgtaatttca ttctggtcta ataatagtgc atcatcactt gttgaatatg taaaccttac
     1801 agcaaaaatt gccgatgaaa ctgacaacga ggaatgtggt cttattacat atagcgcaat
     1861 gataagcggt agtggatatg agggaaatct tccgatggtc tacacaaaag aaacaggggg
     1921 agatttgaca gatgcatttg aaggtatgat agttataaat actaatgata atacattaaa
     1981 aatgtatgca aagggggcgt ggagacaact cgccacttgg taataatttc ataaatacat
     2041 ataataacaa actgtagggg gtttttttta tgattattaa aaatttggtg ttggaagcat
     2101 tgacgaggac agatatactt tctaaacttt tgaactcgaa agaccttcct gtaaggagtt
     2161 cttttcaact tggaaaactt gttaaattgt tatcggatgc tattaaagat tatactgatg
     2221 cgaaacagaa gcttcttgag aagtacgctg aaagggatga agccggtaaa ttgaaggtgg
     2281 aaaacaatca atacatagtt tcatcggaga acattttaca gttcaattcc gatttgaccg
     2341 aattgctgga aattgatatt gatttaccga tggataaagt caaaattaat cttgacaata
     2401 ttccaacggg cctcatctct cctgtagatt tcccattttt ggaaaatttc atagagtttg
     2461 aggaatgagc caaagttata taacagtaaa tcaaaacgaa acaagggcca tagaattact
     2521 gatacgggat cagaatgatg atcccgtata tccagattct gcgtatgcaa gagttgtgga
     2581 tgcttataca gatattcctg tagtgtcaga gatggttgct atggtggatg gtaataaaat
     2641 atacttttta cttactcccg ttgtaactgc agagcctggt atgtatgatg ttatttggag
     2701 aattgtaaaa acagagacaa gtatttatac tttttaccat aagacgaggg taacggtaaa
     2761 tgagttatga ttttttatat acatttgaga atgtctcttt gacattgaat tttgaaatac
     2821 gaagtttcga tgggtctcat ccaatgtatc aggtattgat agattttatt gaggcacagg
     2881 aaatcggaga tatattctaa tggtggttta taaggcaaag aatactataa ccggaaattt
     2941 ttatataggt aaaacttctc ttacccttga aacccgtgaa aagttgagac aaaaaaatga
     3001 aagaatattg ggaacgaaaa agaggaaata aggaatgcgt taccatttta taggacgtag
     3061 tcgtgatagt tacggtaata cattacaggg cgttgatatt tatgtatatt tagccggaac
     3121 tacaacggct gccaaagtct ataccgctat gacaggcggt actgcaatat ctattgcccc
     3181 acaagtcgaa tctgacgcta atggactttt cagtttttat gtagatgatg gtgattatga
     3241 tactactcaa aaatttgata tagttgttgg tgccacaaga tatgaaaata tcgacattta
     3301 caggagaggt attgatggtt cttctggaac atctggcact tcaggaacaa gtggtgaaac
     3361 aggccctata ggtccgcagg gaactggtgg aagttctggt acatcaggaa catccggttc
     3421 atcaggtaca tcaggagaaa ctatatttcc cggtggttac ttacatacac aatcattagc
     3481 atcttctgct tggtctataa ctcataatct cggagagaag tatgttgttg tcgaggtttc
     3541 cgatgaggat gatctttccg tcattccttc ggagatacta tatgtagatg caaataatct
     3601 ggtaattata ttcccatcag atgtcgccgg acaggcaaag attgttgcag gaactggaac
     3661 gagtggcaca agtggtgaag atggaacatc aggtacctct ggttcaagtg gaacgagcgg
     3721 aagctcaggt acaagtggta ttgacggaac atctggtact tcaggtacat caggagaaac
     3781 tacatttccc ggtggatatc ttcacacaca atctgtaccc tcttctgctt ggtctgtaac
     3841 ccataatctt ggagaaaagt atgttgttgt tgaagttgcc gacgatgatg atttgtatat
     3901 cattccttca gatattcttt ttgtagattc gaacaacctg cttattacat tctctgaagc
     3961 aatatcaggc cacgcaagaa ttattgccgg tactggaaca tcaggaattt ctgcaatagg
     4021 tggtgcattt acttacaccc aatcagtacc atctacagca tggtctgtaa ctcacggcct
     4081 cggtgttcaa ccaattgctg tggcggtaat gaacggtgac ggatatggta ttgaaccagg
     4141 cgatattctc tatgttgatg ataacaatgt aatattgaca tttgatcttc ctatttctgg
     4201 aagcgcaaca ttcgtaagtg gtggtggtgc agtaggaaca tcggggactt caggtatttc
     4261 tggaacaagt ggaacttcag gaacttctgg ttcaagtggg agctctggtt cttcaggttc
     4321 atcgggaact tctggaacaa gtgggacctc cggttcttct ggaacggccg gtacttcagg
     4381 tgtggatggt gtatttggtg gaccaagcgc tctttacaaa ttcaacaata catcaacaga
     4441 tgttgatgtt gatcctggtt taggaaaaat cttatttaat aatacatcat ttagttctgt
     4501 aacccgtatc aatgtgaatc attatgatga tggtggtgcc tatctttata actggctcga
     4561 catattcaca ttgacaaaca ataattctct tataaagatt ttcaatgaga cagatccaac
     4621 caagtttgtt atttacagaa ttacaggtct cacgaatcga agtggatata aaacattttg
     4681 ggtatcatat ctttcatcga atggtacaat ctttaataat ggaacaaata tagttctaac
     4741 agtaagtctt atcggttcat cgggttcaag tggaacttct ggaacatcag gaacagcagg
     4801 aacatccggc acctctggtt caagtggaac ttctggaact tctggaagta gtggcacaag
     4861 tggaacttcc ggaacttcgg gtacatcggg aacagcggga acatctggaa gtagtggaac
     4921 atctggaagc tctggatcaa gtggaacatc tggaagtagt ggaacatcgg gttcaagtgg
     4981 ttcatcgggt tcaagtggaa cttctggatg gggaacatct ggaacttccg gaacaagtgg
     5041 tatagatggt acttcaggca tttctggcac ttctggaact tcaggttcaa gtggaacatc
     5101 tggaacatct ggaacttcag gttcaagtgg aacatctgga acatcaggaa catcaggaac
     5161 ctctggaact tctggcacat caggcacatc aggcattgat ggaacatctg gcacatcggg
     5221 cattgatggt acaagtggaa gctctggaac atcgggcaca tcaggatttt ctggcattga
     5281 tggcacttct ggtacaagcg gggaagatgg cacatcgggc acttccggaa caagtggttc
     5341 aagtggaact tctggtttct cagcagcatg gggtgtacca tacagatttg atacagacac
     5401 aactgatact gatcctaata tcggacattt gaaattcaat agtacgatat tttcatctgt
     5461 cacaagaatt tacattgacc tgatggatag atatggccag attttgaccc cttggattga
     5521 tttgatggat gatagcacat cattgtggtc aaagggttat ctcaaaattt ggagggatca
     5581 cgatgaaact gattatgcgt tctttgaaat aaatggaaca actgatattg acccacaata
     5641 tagaaaactt catgttggat tgattgctgc aagttcaacg gaggtatcag gtgcattttt
     5701 ggaagatgat gatcttcaga ttggtttcgt tccaacgggt gatgcaggta catcaggaac
     5761 atcaggactt gctggtcttg gtgcaaatgc acatctctac tggtttgata atgacataac
     5821 ggatagcgat ccaccatccg gtcatctaaa acttaataat gccgatccaa cactcgcaac
     5881 atacatttat gttgatgttt tgaatagatt tggtactgat gttgaggctt ggttggcaag
     5941 tcttgttgac agtaatgctg tcagaataat gaatcagaat agcgtggaaa ttttccatga
     6001 ttatttactc ttgagtgcac caacttctgg agccggttat tataaactca atgtcgggta
     6061 tcttgttgga aatggaacat tgaatgatga ggatgaaatt gttcttggta tcggtaatag
     6121 aggacaaaca ggaacaagcg gaacatcggg aacatcggga acatcaggaa cgagtggaag
     6181 aggatatctc catgttcaat caacacccgc atctacttgg tctataaacc atattttgaa
     6241 ttgccgttat tgtgttgttg aagttactga tgagaatgcg tattcgataa tccccgacac
     6301 tataagattt gatgatgaga ataatctcac gattacattc tccgaagcta tctccggtcg
     6361 cgcatttatt tcttccggtg gtggacttgc tgtaggtgaa ttggcatata cattctccac
     6421 tccggtaaca gcatggaata ttacacataa tcttggaaac aagtatgtca atgttcaatg
     6481 tgtagatggt gatgattatt atattgaacc acaaagtata caatttattg atgaaaataa
     6541 tattgaaata acattctcta caccagtttc aggtgtctgc tctattgttg gtggagtagg
     6601 aacaattgga acatatggta gtagtggaac atcaggttct tctggaacat ctggtgaagg
     6661 aacccccacc ggcgttatat tgccctatat tggaacaacg gccccggcag gatggcttct
     6721 ttcagatggt aaaacaattg gaaatctttc ttccggtgca actggaagag caaatgcaga
     6781 cacacagaca ttatttgaac ttctctggaa ctcaactaca aatacagaac ttgtaattca
     6841 agatagtgcg gggtctccaa ctacgagagg tgtcacggct gctcttgact ttactgcaaa
     6901 caagagaatg cctcttcctg acctgagagg tagaatacca ctcggtaagg acaatatggg
     6961 tggaagttct gctaatgttg tgacagctcc tgcagcagat ttgattgctg gtgagggcgg
     7021 agcagaaaat catacattga cagaagcaga aatgccaagt catagacatt atacaccgac
     7081 aattacgaga actgtctatg attttactgg tggagatcag ggttacggac aggatacacc
     7141 tacgggttct ggtcccaatg
//
LOCUS       DAOTQL010000093         7124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_8806_length_7124_cov_5.305135, whole genome shotgun sequence.
ACCESSION   DAOTQL010000093 DAOTQL010000000
VERSION     DAOTQL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8806_length_7124_cov_5.305135"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2175
                     /gene="brxL"
                     /locus_tag="P5293_06140"
     CDS             127..2175
                     /gene="brxL"
                     /locus_tag="P5293_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HRR49518.1"
                     /translation="MIILDDLDKKALDLFRGFVVKKDLVGIIKGGANVPAFVLEYLLA
                     NTCSTENEEKLKEGMENVKTILRNHYVNPEESTLIQSKLRERGRYKIIDKISVDLDPQ
                     RDRYWANISNSNIKKATISDELVKSHEKLLLGGIWAIIEMEYDPMITIGSAVYPFLVR
                     DIKPIQLSTFDNTKISEKRKEFTKDEWKKLLLRSAGYEPYSEGLDDRKLNLLLCRLIP
                     LVEANFNMVELGPRSSGKSYIYKEITPYAILISGGQGSVAQLFVNNTTGRVGAVGLWD
                     AICFDESTDKLFKDRDAIPLMKDYMESGSFSRAKGSEISGSASVILNGNINQPVETVL
                     QTSHLFSPLSEEVNHDTAFLDRINLFLPGWEITKFSPSNFTTHFGFSTDFFSEILKSQ
                     RKTTYYDAIDKYFSLGSHLKQRDSKSVRRIVSGFIKLLHPDGNFTKEDIKEYLVIAME
                     MRRRVKEQLKRIGGMEFWDTNFSYIDKETQEEIFVGLQEEKGSSLIESNPLSPGVCYT
                     ATSDGDNTALVKIEVVALKGSGKMNVTGTSSQEVKENIKNTYNYLRANERSILSDQHS
                     LSAYDINIQISNLMGAYVSGGIGSAVYIAILSAIYKKNLKPGLAVLGNISIGGAIERA
                     LHFPDKVTLLSENGAKTVLVPMDNLSEISTIPTSILGKTDVPFYGNSQMLLQKGVLGE
                     "
     gene            2617..3885
                     /locus_tag="P5293_06145"
     CDS             2617..3885
                     /locus_tag="P5293_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49519.1"
                     /translation="MKPSSIIILKSAIIIFIIALVSKVSGQEISHSGYVDTLQFKSKQ
                     YFSNPNYGAQLENYMGTRSISFEFPGKSAKQLYNACLKLFRGYTEKIEFIENRIIDSY
                     YIKKVEYSDPIGAVIIYLYNYMVICDDEKITIENPHIATLYTEFNANTGKVLKDYISV
                     ASYISLLQEFSSKDKGGKLKEKRDELRAFLSSNVKIGYQALSNQLNHFTDSLLTSINN
                     GIELDSLEWISDAYAPVFTLSIEGLTASNNRNYMVIKTPEKNSDQIKNKTNALVNFVH
                     MDEPIEYYSSIINNRYAFYEIRDIPAYVVDSYFDKGIINFSDIYSKCDFGDFQKFVVK
                     KTIVATDKVYGVANFEFEITYAPGLVKISTPTISKIEDGIYDWMNKENAYRSIFSTPD
                     NRVLQPVWKKAVETYFNELFYFLFKYYSQN"
     gene            complement(3902..3975)
                     /locus_tag="P5293_06150"
     tRNA            complement(3902..3975)
                     /locus_tag="P5293_06150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3939..3941),aa:Arg,seq:tct)
     gene            complement(4012..4659)
                     /locus_tag="P5293_06155"
     CDS             complement(4012..4659)
                     /locus_tag="P5293_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRR49520.1"
                     /translation="MESAHFIEWCLENLNYWTVTLLMAIESSFIPFPSEVVIPPAAYK
                     AAAGELNVFLVVFCGTTGALIGAYFNYFIAYYLGRPLIYKFVNTRFGHMCLLDENKVH
                     KAEAYFIKNGAISTFVGRLIPAIRQLISIPAGLAKMNFKQFTFYTVLGALLWNTILAA
                     VGYSLHSVVPKDQLMQKVQEYSREISNGMIILGILLVLYFIYKGFAKPKNGVKAE"
     gene            complement(4668..5912)
                     /locus_tag="P5293_06160"
     CDS             complement(4668..5912)
                     /locus_tag="P5293_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR49521.1"
                     /translation="MSEENFLDYNEEESELSEQVKICREAFSRGELAKLDFSEEEFDT
                     LLGFFADEYEDKLLLALSRIACQRYPYSSELIVKYSDALIINEEPEKAVEILERQLGL
                     NSSNSDVYFLLGRGEIKMKNFEIAQQYINHALIFSPDGQDDMLSSIALDFMEAAQYDR
                     ALEYLMRVYKNNSEDIIVCNDIAFCLERTERYEESLEYYYKCIEADPFNSNIWFNIGT
                     VNATIEEFDKAIEAFDYSLALEPDNSSVMYNKAVVFLNLSQYDKAAEILEDFLVYEPE
                     DYSALTAIADCYLKLGQGDKAEKYMKMLLEFDQNNIEAYSSLAYISLVRQEYDKSLDY
                     LRILIGKENVNYNLLEDDLYIAFQKTGLPEFLVYYVAAEYFIDDQAGLLSGFEQLLEF
                     DEVWQNMLYRLVPELNFFNQNT"
     gene            complement(5912..6409)
                     /locus_tag="P5293_06165"
     CDS             complement(5912..6409)
                     /locus_tag="P5293_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49522.1"
                     /translation="MDKAVAKRKVQFVSLDMIKRITFIFDVTDEDSMEAVKFLTSYAR
                     DNKIEYHGMAINLGKNNSSEAVLGHGIKLITKKNLSKLGVPDFNIVQRFINDCSDLFI
                     DFSNKYCFVNEYLALSSMSGFKVGRHTPEPNPYDLVIKSDEEKNSALDFVKKLFFYLN
                     SIKPL"
     gene            6515..>7124
                     /locus_tag="P5293_06170"
     CDS             6515..>7124
                     /locus_tag="P5293_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR49523.1"
                     /translation="MISDISELTLTDLFSKSIIDYENRPAFSLTGKTSVTYGEVSQKV
                     EKLIEIFNRYGLDEGDKISILGHNMPNWPISYFAVTTSSRVVVPILPDFTAFEIVNIL
                     QHSESKALIVSKRLQYKIPKHIYNELNLIISMDDFEIIQSSEKEKEVITSTPHPDDLA
                     AIIYTSGTTGTSKGVMLSHRNLVSHEYMANRLFEIKKEDVFLS"
BASE COUNT         2454 a         1280 c         1241 g         2149 t
ORIGIN      
        1 catttggcgg tcatagtgaa attattgcag tcatagtaga tgcaagtaca caagaacaaa
       61 ttgattctgt aaaagtaaaa aaatcgaacg ccagggatct ggacggccta atgtaaaaaa
      121 tgaatcatga taatcttaga cgacttagat aaaaaagctt tagacctctt cagggggttt
      181 gttgtaaaaa aagacctggt aggcatcatc aaaggcggtg ctaacgtacc tgcatttgta
      241 ttggaatatt tattggccaa cacttgcagt accgaaaatg aagagaagct gaaagaaggg
      301 atggaaaacg tcaagaccat acttcgcaat cattacgtca atcccgaaga aagcacattg
      361 atccaatcaa aacttcgtga aagaggtcgc tataaaatta tcgacaaaat atcagttgac
      421 ttagatccgc aaagagaccg atattgggct aacatcagca atagcaatat caaaaaagcc
      481 acaatcagcg atgaactggt aaaaagccat gagaaattgc tgttaggcgg tatttgggca
      541 atcatcgaaa tggaatacga cccaatgatt actataggca gtgctgtata tcctttttta
      601 gtcagagata taaagcccat acaattgtca actttcgaca ataccaaaat ttcggaaaaa
      661 cggaaagaat tcaccaaaga tgagtggaaa aaactattgc taagaagtgc aggatacgaa
      721 ccgtacagcg aaggattgga tgatagaaaa ctaaacttgc ttctctgccg cttaatacca
      781 ttggtggaag ccaacttcaa catggttgaa ttgggcccac gttcatcagg caaatcttat
      841 atctacaaag aaataacgcc ttatgccata ctcatatcag gtggccaggg atcagtagcc
      901 cagctttttg taaacaatac caccggcagg gtaggtgcag taggtttgtg ggatgcaatt
      961 tgttttgatg aaagtaccga taaactattc aaagatcgtg atgccattcc gttaatgaaa
     1021 gactacatgg agtcaggttc tttttccaga gccaaaggta gcgaaatttc aggttcggct
     1081 tcagttatct taaatggaaa tatcaatcaa ccagtagaaa ctgtattgca gacatcacat
     1141 ttgttcagtc cgctgtcaga agaagtaaac cacgacaccg catttttaga cagaataaac
     1201 ttgtttctgc ccggttggga aataactaaa tttagtcctt caaatttcac aactcacttt
     1261 ggtttcagca ccgatttctt ttccgagata ttaaagtctc aacgcaaaac aacttactat
     1321 gatgctatcg ataaatattt cagtttaggt tcacacttaa aacaacgtga ttcaaaatcg
     1381 gtaagacgta ttgtatcagg tttcatcaag ctacttcacc ctgacggtaa tttcaccaaa
     1441 gaagacatca aagaatactt ggtgattgca atggaaatga gaagaagggt aaaagagcag
     1501 ttaaagcgaa ttggaggaat ggagttttgg gacactaatt tttcatatat cgataaagag
     1561 acacaagaag agatctttgt tggcctacaa gaagaaaaag ggtcatcatt aatcgaatcc
     1621 aatcccctat cacctggggt ttgttatacg gctacaagcg atggcgacaa tacagctttg
     1681 gtgaagattg aagtagtcgc cttaaaagga tcgggcaaaa tgaacgtaac aggaactagt
     1741 agccaggaag taaaagagaa tatcaaaaat acctacaact accttcgggc aaacgaaagg
     1801 agcattttaa gcgatcaaca ctcattatca gcatacgata tcaacatcca aatcagcaac
     1861 ctgatgggag cttatgtaag tggtggaata ggcagtgctg tttatatcgc catcttatca
     1921 gccatctata aaaagaacct caaaccgggt cttgcagtat tgggtaacat atccattggc
     1981 ggtgcaattg aaagagcatt acactttcca gataaagtga ccttgctttc cgaaaatgga
     2041 gcaaaaacag tcttggtgcc aatggacaac ttatctgaaa taagcaccat tccaacttca
     2101 atcttaggta aaacagatgt gcctttttat ggcaatagtc agatgttgtt gcagaagggg
     2161 gtattggggg agtgagttga tgcgtaagca tccgagagtc tgtgcaaaaa ccacttttta
     2221 aacgaagtgt cttttaatcg tttatttctt taagctttta tttaagtatt ttaagccctc
     2281 atcaagttga tctttatata atgggaatta ttttcacaat taaattggcg gcaaaatggc
     2341 gtataataat ataaataatg agaataatga cttttgaatt cgacttttat ggcttttctt
     2401 ttctactaca tgctttatag aagacatagc ggagtgagaa aagaacttaa cgtaaaaacg
     2461 ccttttgatg ctattgaaaa atggtttgaa attaatccga aaatctttat agaaaaccct
     2521 ttgcagttta aaaacaaaat tttatattta taatcgaata aaagtccaag ttttcataaa
     2581 caaccttgtg aaacttgaca tttaatacct taaaaaatga aaccttcttc aataataatt
     2641 ttaaaaagtg ctattattat ttttattata gcacttgttt ccaaagtatc cgggcaagaa
     2701 ataagccact caggttatgt tgacactctg cagttcaaat caaaacaata tttttcaaat
     2761 cctaattatg gagcccagtt ggaaaactat atgggaacta ggtctatctc ttttgaattt
     2821 cctggcaaaa gtgccaagca actttacaat gcatgtttga aactatttag aggatatacg
     2881 gaaaaaattg aatttataga aaaccgaatt attgattcct actatataaa aaaagttgaa
     2941 tattcagacc caattggagc agttataatt tatttgtaca attacatggt gatatgtgat
     3001 gatgaaaaaa taactattga aaatcctcat attgccacct tatacacaga attcaacgcc
     3061 aatacaggga aagttttaaa ggattatata tcagtagctt catacatatc tttgctgcag
     3121 gaattcagct caaaagataa aggaggaaaa ttaaaagaaa aacgtgatga attaagggca
     3181 ttcctgtctt caaatgtcaa aattggttat caagcgttaa gcaatcaact taatcatttt
     3241 actgacagcc ttttaacaag tattaataat ggcatagagt tagactcatt ggaatggatt
     3301 tcagatgcct acgcacctgt ttttacgttg agtattgaag gactaacagc aagcaataat
     3361 cgcaattaca tggtcatcaa gacacccgag aaaaacagcg atcaaataaa aaataaaaca
     3421 aacgctttag tcaatttcgt tcatatggat gaaccaatag aatattatag cagcattatc
     3481 aataacaggt atgcatttta tgaaataaga gatattcctg cttacgtagt tgattcctac
     3541 tttgataaag gaattattaa ctttagtgat atctattcaa agtgtgattt tggtgacttc
     3601 cagaaatttg tagtcaaaaa aacaatcgtg gcaacagaca aagtatatgg agttgcaaat
     3661 ttcgaatttg aaattactta tgcaccagga cttgtgaaaa tatctactcc gacgattagc
     3721 aaaattgaag atggaattta cgattggatg aataaggaaa atgcatatag atcaatcttt
     3781 agtactcctg acaacagagt gctccaacct gtctggaaga aagcagttga aacatatttt
     3841 aatgagcttt tctatttttt gttcaaatac tactcacaaa attgatttcc ttaacttgaa
     3901 gtgattcggt tgggattcga acccaagacc cacagcttag aaggctgttg ctctatccaa
     3961 ctgagctacc gaaccttttt tgagtttgca aatatagtaa ataatttgga actattcggc
     4021 tttgactccg ttttttggtt tggcaaatcc cttgtaaata aagtaaagaa ctagtaaaat
     4081 gccgagtatt atcatcccat ttgatatctc tctactatat tcctgaactt tttgcataag
     4141 ttgatccttt ggaacaacgc tatgaagaga gtagccaacc gctgcaagaa ttgtattcca
     4201 taacaaagct ccaagaacag tataaaatgt aaattgtttg aaattcattt ttgcaagtcc
     4261 cgccggtatt gaaataagct gtctaatagc cggtatcagc ctgcccacga atgttgaaat
     4321 agctccattt tttataaaat aagcttctgc tttgtgtact ttgttctcgt caagcagaca
     4381 catgtgtcca aaacgtgtat ttacaaactt gtatattaaa ggccttccaa gataatatgc
     4441 aatgaaataa ttaaaatacg ccccaattaa tgcgccagtc gtaccacaaa aaaccaccag
     4501 aaaaacattc aattctcctg cagcagcctt atatgctgca ggaggaatca caacttctga
     4561 tgggaaagga ataaaagaac tttcaattgc cataagcagt gttactgtcc aataattaag
     4621 attttcaagg caccattcaa taaagtgtgc agattccata aagagtttta tgtgttttga
     4681 ttaaaaaaat taagttccgg cacaagtctg taaagcatat tttgccacac ttcatcaaat
     4741 tccaagagtt gttcaaaacc tgaaagaagc cctgcctgat catcaataaa atattctgct
     4801 gcaacataat ataccaaaaa ctcaggaaga ccggtttttt gaaatgcaat atacaagtca
     4861 tcctcaagca gattgtaatt tacattttcc ttgcctatta agattcttaa ataatcaagc
     4921 gatttatcat attcttgcct gactaaagat atatatgcaa gagaagaata agcttcaata
     4981 ttattttgat caaattcaag aagcattttc atgtatttct cagccttatc accttgccca
     5041 agtttaagat agcaatctgc aatagccgtc agtgcagaat agtcttcagg ttcataaacc
     5101 agaaaatcct cgaggatttc tgctgccttg tcatactggg ataaattgag aaataccaca
     5161 gctttattgt acatgacaga tgagttatcg ggttcaagag ccaaagaata gtcaaacgcc
     5221 tcaatagcct tgtcaaactc ttcaattgta gcattgacag taccaatatt gaaccagata
     5281 ttactattaa aagggtccgc ttcaatacat ttataataat attccagact ctcctcgtat
     5341 cgctcagttc tttcaaggca aaaagcaata tcattacata caataatatc ctcagaatta
     5401 tttttatata ccctcattaa atattccaat gcacgatcat attgagcagc ttccataaag
     5461 tcaagagcta tagaagacaa catatcatct tgtccatcag gcgagaatat taatgcatga
     5521 tttatatatt gctgtgcgat ttcaaagttt ttcatcttaa tttcacctct gccaagcaag
     5581 aagtagacgt cactattact tgaatttaaa ccaagctgac gctcgaggat ttctacagct
     5641 ttttcaggtt cttcattgat tataagcgca tctgaatatt taacaatcaa ttcggaagaa
     5701 tagggatatc tttgacaggc aattcttgat aaagcaagca agagtttgtc ttcatactcg
     5761 tcagcaaaaa aaccgagtaa ggtgtcaaac tcctcttctg aaaagtctag ttttgcaagt
     5821 tctcctctgc tgaaagcttc tctgcaaatc tttacctgct ctgataattc actctcctcc
     5881 tcgttgtaat ccaaaaagtt ctcctctgac attataatgg tttgatagaa tttaagtaaa
     5941 agaataattt tttcacaaaa tcaagtgcac tgttcttctc ttcatcactt tttataacaa
     6001 ggtcatacgg atttggttca ggggtatgcc tgccaacctt aaaaccagac atggatgaaa
     6061 gtgcaagata ttcattgaca aaacaatatt tgtttgagaa gtctataaat aaatcgctac
     6121 aatcgttgat aaatctttgt acaatattaa aatccggaac tcccaatttt gaaagatttt
     6181 tttttgtaat aagcttgatt ccgtgtccaa gcactgcttc tgaagagttg tttttaccaa
     6241 ggtttatggc cattccatga tattcaattt tattgtctct tgcatatgat gtaaggaatt
     6301 ttactgcttc catactatcc tcatcagtga catcaaaaat aaacgttatt ctcttgatca
     6361 tgtcaagaga gacaaattga acttttcgct ttgcaacagc tttatccaaa attttttttt
     6421 gcagtctctg tctgaacatc acaaattaat attttttact ggttacaaaa ataacaaatt
     6481 ataattgtta tctttgaact gtcatcaaaa tcttatgata agcgatatct cagaattgac
     6541 tttaacggat ctcttttcaa agagtattat tgattatgag aaccgtccgg cattttcact
     6601 cactggaaaa acgtctgtga catatggtga agtcagccaa aaagttgaaa aactcataga
     6661 aatatttaac agatacggtt tggatgaagg cgataaaatt tcaatactgg gacataacat
     6721 gccaaactgg cctataagct attttgctgt taccacttct tccagagttg ttgtacccat
     6781 tttaccagac tttactgcat ttgagattgt taacattctg caacattcgg aaagtaaggc
     6841 tctgattgtt tcaaaaagac ttcaatataa aattccgaaa catatttaca acgagctcaa
     6901 tctcattata tctatggatg attttgaaat catccaatct tccgaaaaag agaaggaggt
     6961 gattacatct actcctcatc ctgatgacct tgcagcaata atttatacat ctggtactac
     7021 tggaacttct aagggagtta tgcttagcca tagaaatctt gtctctcatg aatacatggc
     7081 aaacaggctt tttgagataa aaaaggagga tgtttttctc tcaa
//
LOCUS       DAOTQL010000094         6850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_9212_length_6850_cov_5.145843, whole genome shotgun sequence.
ACCESSION   DAOTQL010000094 DAOTQL010000000
VERSION     DAOTQL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_6850_cov_5.145843"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="P5293_06175"
     CDS             <1..1471
                     /locus_tag="P5293_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRR49524.1"
                     /translation="DVLEYLDETYIHKKSAPVKLLYIESISYPEKLLKHAVSLINKGA
                     KIAAIKSGTSDAGSRAASSHTGAMATPDSAVSALFQKAGIVRCYSRTELVTVGAVMLN
                     KLPNGNKVAIITHAGGPAVMLTDTLSEYNISIPHISGEKADNLLKELYNGSSTSNPID
                     FLATGTASQLAKIIDACEKDFDVDSMVVIFGNPGLTSVYDVYETLFNKIKECNKPIFS
                     ILPSIVNAKDEIAKFKEMGGICFPDEVIFGNALGKVIKSHSPLFDSQLPPVDKKIIRD
                     VIKKAENGYLSPLQVQTLLDAAGIGRVKEIVVDKMNQLRKAALEIGFPVVMKVVGPVH
                     KTDVGGVILNVTDEETMISEFNRMMNIPGTHAIMLQPMLTGIQIFIGAKREEKFGHLV
                     MCGLGGIFIEVLNDVQSALSPVSKSEAKIMISSLKGYKMIKGSRGIEGVNEAIFIENI
                     RRVSALCNAAPEIYEMDLNPLLGNSKQIIAVDARIRIEK"
     gene            1718..1939
                     /locus_tag="P5293_06180"
     CDS             1718..1939
                     /locus_tag="P5293_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRR49525.1"
                     /translation="MALKGAIVVDNEKCKGCGVCVVNCPTNVIALAKEVNSKGYNFAY
                     MVLPESCTGCTNCAAVCPDSCITVYRVKI"
     gene            1961..3040
                     /locus_tag="P5293_06185"
     CDS             1961..3040
                     /locus_tag="P5293_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRR49526.1"
                     /translation="MAEKILMKGNEAIAHAAIRCGCDGYFGYPITPQSEVMETLMEEK
                     PWETTGMVVLQAESETSAINMLYGGACCAKRVMTSSSSPGISLMSEGMSYLAGAELPC
                     LVVNVVRGGPGLGTIQPGQGDYFQAVKGGGHGDYKNIVLAPSSPQEMYNFVDKAFELA
                     FKYRNPAMILSDGIVGQMMETVEMGPMKPRLSNEEIIKRYPWATTGKTKERKQNVITS
                     LAMEAEVNERNNLRLQAKYREIENNEVIFETIHCEDANHLMIAYGCMSRICENAVEQA
                     RKEGIKVGLLRPITLFPFPTKEIQKLVPQLDTIMTVELSAGQMVEDVRLAVNGAIPVN
                     FYGRMGGVVPSPEEVLEAFKKSILK"
     gene            3059..3820
                     /locus_tag="P5293_06190"
     CDS             3059..3820
                     /locus_tag="P5293_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRR49527.1"
                     /translation="MDIKDIIKPENKVYGKSSVLTDNIMHYCPGCSHGTVHKLIAEVI
                     EEMGIQEQTIGISPVGCAVFAYNYVDIDWQQAAHGRAPSLATATKRLHPEKYVFTYQG
                     DGDLASIGTAEIMHACNRGENIVVIFINNAIYGMTGGQMAPTTLIGMKTATTPYGRDQ
                     KLHGNPLKITELIAQLEGTCYVTRQSVHNPANVRKAKKAIRKAFENSMMNKGTSFVEI
                     VSTCNSGWKLTPVQSNEWMVKNMFPEYPLGDIKDR"
     gene            3836..4378
                     /locus_tag="P5293_06195"
     CDS             3836..4378
                     /locus_tag="P5293_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRR49528.1"
                     /translation="MKEEIIIAGFGGQGVLSMGKILAYSGIMQGMQVTWMPSYGPEMR
                     GGTANVTVILSDTKISSPLLSKFDTAIILNQQSMDKFENSIKPGGMMIYDPNGIVRKP
                     VRKDIKIYSIDAVEISNELGNSKAYNMVVLGAYLSKKPIVSMENIIKGLKKSLPERSH
                     HLIPLNEKAIKVGMEKVKEG"
     gene            4462..4773
                     /locus_tag="P5293_06200"
     CDS             4462..4773
                     /locus_tag="P5293_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49529.1"
                     /translation="MAHYSCMSGDYHIVIDSIKSKIELLISRYEQVVAENQRLLSELL
                     DCKSALDESNNKIKELEEKINKLQLSEAFKASSQDVKEAKQKINMIVKEINKCIALLN
                     D"
     gene            4766..5074
                     /locus_tag="P5293_06205"
     CDS             4766..5074
                     /locus_tag="P5293_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRR49530.1"
                     /translation="MINIHMEQQSIRICIADRYYPLKVAVNDEEKIRSAARIINEKVD
                     QYKSRYVSRDNQDALSMAILQFVIRLIEAEQKEESKQIVEELQNLDTLLDEYIKVNIT
                     "
     gene            5311..6846
                     /gene="rny"
                     /locus_tag="P5293_06210"
     CDS             5311..6846
                     /gene="rny"
                     /locus_tag="P5293_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRR49531.1"
                     /translation="MEVLLAIVLPAIASFILTYYITNNFILKKRREEILKSAEQEGEN
                     IKKEKIFQAKEKFLQLKGEHEQQILERNNQIIQVENKLKQRELVLNQQNAELQKKLKE
                     IESAREHIRNQTEFLEKKQEEYEKLRIEAIQKIENIAGLSAQEAKNQLIETMKAEAKS
                     EAMSYISEVMDEARLTASKESKRIIINTIQRIAPETAIENAVTVFNIESDEIKGRIIG
                     REGRNIRALEAATGVEIVVDDTPEAILLSAFDPVRREVARLALHQLVTDGRIHPARIE
                     EVVAKVQKQLDDEIMETGKRTCIDLGIHGLHVELVKLIGRMKYRSSYGQNLLQHSREV
                     ANICATLASELGLNPKTAKRAGLLHDIGKVSDEDPELPHAILGMKLAEKYKEKPDVCN
                     AIGAHHEEVEMTSLISPLVLVSDAISGSRPGARREVIESYIKRLKEMEDLALSYPGVT
                     KTYAIQAGRELRVIVGSEQVSDKESEQLSLDIAKKIQDEMVYPGQVKITVIRETRAVS
                     FAK"
BASE COUNT         2438 a         1113 c         1430 g         1869 t
ORIGIN      
        1 agatgtactt gaataccttg atgaaacata tattcataag aaaagcgccc ctgttaagct
       61 tctttacata gagagcataa gctatccgga aaaattgtta aaacatgccg tttcacttat
      121 caataaagga gcaaaaattg ctgcaattaa atccggaaca agtgatgccg gaagccgagc
      181 agcatcttca catacaggag caatggcaac tcctgattct gctgtttctg ccctttttca
      241 aaaagcaggt attgtaaggt gttacagccg aacagaactt gttacagtag gcgccgtaat
      301 gcttaataaa ttgcccaatg gaaacaaagt tgctattata actcatgccg gcggaccagc
      361 agtgatgtta acagatactc tttctgaata caatatatca atcccccaca tttcagggga
      421 aaaagccgat aatctcctca aagagctcta caatggttct tcaacttcca atcctattga
      481 ttttcttgct acggggacag cgtcacaact tgcaaaaatc atagatgctt gcgagaaaga
      541 ttttgatgta gattcgatgg ttgtcatatt tggcaaccct ggcttaacca gcgtttatga
      601 tgtttatgaa actttattca ataaaataaa agagtgtaat aaaccgatat tttcaatact
      661 tccttcaata gtgaatgcta aagatgagat tgccaagttc aaggaaatgg gtggaatatg
      721 ttttcccgac gaagttatat ttggtaatgc attgggaaaa gtaataaagt ctcatagccc
      781 actttttgac tctcaacttc cacctgttga caaaaaaatt attcgtgacg taattaaaaa
      841 agcagaaaac ggatatctgt cacctttaca agttcagact ctgctcgatg ctgcaggtat
      901 tggcagggta aaagagattg ttgtagataa aatgaaccag cttagaaaag ctgctctcga
      961 gataggattt cctgtagtaa tgaaagttgt cggtcctgtt cacaaaacag atgtaggagg
     1021 agttatcctt aatgtgactg acgaagaaac gatgatttca gaatttaaca ggatgatgaa
     1081 tatacccggt acacatgcta taatgttgca accaatgctt accggcatac aaatcttcat
     1141 tggtgcaaaa agagaggaaa agtttggtca tcttgttatg tgtggtctcg gtggcatatt
     1201 tattgaagtt ttaaatgacg ttcagagtgc actgtcacca gtgtcaaagt ctgaggctaa
     1261 aataatgata agtagtctta agggttataa gatgataaaa ggaagtagag gcattgaagg
     1321 agtcaatgaa gccatcttca ttgaaaatat caggagagtt tcagctcttt gtaatgcagc
     1381 tccggaaatt tatgaaatgg atcttaatcc tcttctcgga aattcaaaac agataattgc
     1441 agttgatgca agaattagaa tcgaaaaata aagaaaataa tttttttaga gttatttggc
     1501 agaaattcag gaatcttgac ccaaaaattc aaggagcctt aattcttatt ttggtgttac
     1561 taatcggaat tattattaga tggagtttta taattgaaag tgttataaga agttttaatt
     1621 ttttttctaa gtaattatag ctatttaata ataaattatt gcattttata gcataattat
     1681 atagttttgt aaaaacaatt ttaaaaatca taataatatg gcattaaaag gagcgatagt
     1741 agttgacaat gaaaaatgca aaggttgcgg tgtttgcgtt gtgaattgtc ctactaatgt
     1801 aattgccttg gctaaagagg taaacagcaa gggatacaac tttgcttata tggtattacc
     1861 agaatcctgt acaggttgca ctaattgtgc agcagtatgt cctgacagct gtataacagt
     1921 atacagagtc aaaatttaat gaagttaaaa actaagtaat atggcggaaa agattttaat
     1981 gaaaggcaac gaagccattg cacacgctgc aatacgttgt ggatgtgatg gctactttgg
     2041 ctatcctatt acacctcaat cagaggtcat ggagacactt atggaagaaa agccgtggga
     2101 aactacagga atggttgttc tgcaggcgga aagtgaaaca tccgcaataa atatgctgta
     2161 tggtggagca tgttgcgcaa aaagggtcat gacctcttca agtagtcccg gaatcagttt
     2221 gatgagtgaa ggtatgagtt atttggcagg tgccgagttg ccatgtctcg tagttaatgt
     2281 tgtgcgtggt ggcccaggac ttgggacaat tcaaccagga cagggtgatt atttccaggc
     2341 agtaaaaggt ggaggacatg gagattataa aaatatagtt ttggctccgt cttcacccca
     2401 agaaatgtac aattttgtag acaaagcatt tgaacttgct tttaagtacc gcaatcccgc
     2461 aatgattctt tcagacggca ttgttggtca gatgatggag actgttgaaa tggggccaat
     2521 gaaacctcga ttgtccaacg aagagattat taagagatat ccttgggcaa ccaccggcaa
     2581 gacaaaggaa aggaaacaaa atgtgatcac ttcattagca atggaagcag aagtcaatga
     2641 aaggaacaat ctcagacttc aagcaaaata cagagaaatt gaaaataatg aagtaatttt
     2701 tgaaactatt cactgtgaag atgcaaatca tctgatgata gcatatggct gtatgtcgag
     2761 aatatgtgaa aatgctgttg aacaggcaag aaaagaaggg attaaggtcg gacttcttcg
     2821 ccctatcact ctctttcctt ttccaactaa agagattcaa aaattagttc ctcaacttga
     2881 cacaatcatg acagttgagt taagtgccgg gcagatggtt gaagatgtaa ggcttgctgt
     2941 taatggtgct atacctgtta atttttatgg aagaatggga ggcgttgttc cttcacctga
     3001 agaggtattg gaagccttca aaaaatcaat tttgaaataa tttgcaaaac ggaatatcat
     3061 ggatattaaa gatattatta aaccggaaaa taaagtatat ggtaaaagtt ctgttctgac
     3121 tgacaatata atgcattact gtcccggttg ctcccatgga actgtgcata aactcattgc
     3181 agaagttata gaggagatgg ggatccagga acagactata ggaatttccc cggttggctg
     3241 tgcagttttt gcatacaatt atgttgacat agactggcaa caggcagctc atggacgggc
     3301 accttcactt gccactgcca ccaaaagact tcatcctgaa aaatatgtat ttacttatca
     3361 gggagacggg gatttggcat ccattgggac agccgaaatt atgcatgcat gtaacagagg
     3421 agaaaatatt gttgttattt tcatcaataa tgccatttat ggtatgacag gtggacaaat
     3481 ggctccaaca acacttatcg ggatgaagac agcaacaact ccatatggca gagatcaaaa
     3541 attgcacggt aatcctttaa aaataactga gctgatagca caacttgaag gcacatgcta
     3601 cgttacacga cagtcagttc acaatcctgc caacgtaagg aaggccaaga aagctattcg
     3661 taaggcattc gaaaactcta tgatgaataa gggaacttca tttgttgaaa tcgtcagtac
     3721 atgtaattct ggctggaagc ttacaccagt tcagtcaaat gaatggatgg tcaaaaacat
     3781 gttccccgaa tacccattag gagacattaa ggacagataa aaactgaaca acaatatgaa
     3841 agaagagata ataatagcag gttttggtgg acagggagtc ctatcaatgg gtaaaattct
     3901 tgcatattca ggtatcatgc aaggaatgca ggtaacctgg atgccttcat acggtccgga
     3961 aatgagagga ggtacagcaa atgtaactgt aatactgagc gatacaaaaa taagttctcc
     4021 acttctcagt aaatttgaca cagctataat tctcaatcaa caatcaatgg ataaatttga
     4081 aaatagtatc aaacctggtg gcatgatgat ctatgatcca aatggtattg tacgcaaacc
     4141 cgtacgcaag gatattaaaa tttactccat agatgctgtt gagatatcaa acgagctagg
     4201 aaattcaaaa gcttataaca tggttgtact tggtgcatat cttagcaaaa aaccaatagt
     4261 aagcatggag aacattataa agggtttgaa aaaatctttg cctgaaagaa gccatcattt
     4321 gatacctctg aatgagaaag caattaaagt tggtatggaa aaggtaaaag aaggataatt
     4381 ttactttttt tcactataaa gtgcacttaa attaatttag gtgcactttt tgttataatt
     4441 tatttaatta ctatctttgc tatggcgcat tatagttgta tgagtggaga ttatcatata
     4501 gtaatagaca gtattaaaag taaaatagaa ttactaatat caagatacga gcaggtagtt
     4561 gctgaaaacc aaagattatt atctgaactt cttgactgta aaagcgcctt ggatgaaagt
     4621 aataacaaaa ttaaggaact ggaagaaaaa attaataaat tacagttaag tgaggctttt
     4681 aaagcatcat cacaagacgt taaagaggca aaacagaaga taaacatgat tgtgaaagag
     4741 attaacaagt gtatagcatt gttaaatgat taacatacat atggaacagc agtcaattag
     4801 aatatgtatc gccgacagat attacccgct taaggtagcg gttaacgacg aagaaaaaat
     4861 acgctcagca gctcgtatta ttaatgagaa agttgaccag tacaaaagca gatatgttag
     4921 tcgtgacaat caggatgctc tttcaatggc tatcctccag tttgttatcc gccttattga
     4981 ggcagaacag aaagaggaaa gcaaacaaat agtcgaagaa cttcaaaacc ttgatacgct
     5041 tctggatgag tacattaaag tcaatatcac ttgactgaat atgagaagtt agccgttggt
     5101 ttagctcttt gccaaaatca acactaataa cataccgagc aaactcgaca gtcaaaaaca
     5161 ggcctaggtt acccttcggg ggattccgag ttcgggacgt tggaagtttg cgacatatgt
     5221 cggagctcaa atcttattta attaaggttt tcgttaaata gctttaccaa cggctttttt
     5281 aatgtacaat gaaaaacata tttttacaca atggaagtac tattagcaat cgttttacct
     5341 gctatagcaa gttttattct gacttactat attacaaata attttattct aaaaaaacgc
     5401 agagaagaga ttttaaaatc agcagaacaa gaaggagaga atataaagaa agagaagata
     5461 tttcaggcaa aagagaaatt tcttcagcta aaaggagaac atgaacaaca aattcttgaa
     5521 cgtaacaacc agattattca agttgaaaac aaactcaaac aaagagaact tgtattaaat
     5581 caacaaaatg cagaacttca aaaaaagctc aaagaaatag aaagtgccag ggaacatatt
     5641 agaaatcaga cagagtttct tgagaagaaa caagaagaat atgaaaagtt aagaattgaa
     5701 gcaattcaaa aaattgaaaa tatcgccgga ctttcagcac aagaggctaa gaatcaactc
     5761 attgagacaa tgaaagctga agctaaatct gaggctatgt cttatattag tgaagtaatg
     5821 gacgaagcaa gactgacagc aagcaaagaa tctaagagga ttattataaa cactatccag
     5881 agaatagctc cggaaacagc aattgaaaac gctgtcacag ttttcaatat tgaaagtgac
     5941 gaaataaagg gaaggattat cggaagagaa gggagaaata tacgtgctct cgaggcagca
     6001 accggagttg aaatagttgt agatgatact cctgaagcta ttttgctgtc tgcatttgac
     6061 ccagtaagaa gagaagttgc aagacttgct ctgcatcaat tggttacaga cgggagaata
     6121 catcctgcaa gaattgaaga agttgttgca aaagtccaga aacaacttga tgacgagata
     6181 atggaaaccg gcaaaagaac atgtattgat cttggcattc acggacttca tgttgaactt
     6241 gtcaagctga tcgggaggat gaaatatcgt tcttcatatg gacaaaatct gctacaacat
     6301 tcaagagaag ttgccaatat ttgtgctaca cttgcatctg aacttggatt aaatccaaaa
     6361 acagccaaaa gagccgggct tcttcatgac ataggaaagg tttctgacga agatcccgag
     6421 ttgccacatg ctattcttgg aatgaaatta gctgaaaaat ataaagaaaa accagatgtt
     6481 tgcaatgcaa taggtgcaca tcacgaggaa gtagagatga catctctaat atcgcctctg
     6541 gttcttgtca gcgatgcaat atcaggatcc agacctggtg caagaaggga agttattgaa
     6601 tcatacatca agaggcttaa agaaatggaa gacctggcct tatcctaccc tggtgtaaca
     6661 aaaacttatg caatccaggc tgggagagaa ttaagagtca tcgtaggaag tgaacaagtt
     6721 tctgataagg aatcagaaca attgtcactt gacatagcta aaaaaattca ggatgaaatg
     6781 gtctatcccg gacaagttaa gataaccgtt attagagaaa caagagctgt aagttttgct
     6841 aaataagcag
//
LOCUS       DAOTQL010000095         6606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_9620_length_6606_cov_6.104717, whole genome shotgun sequence.
ACCESSION   DAOTQL010000095 DAOTQL010000000
VERSION     DAOTQL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9620_length_6606_cov_6.104717"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..182
                     /locus_tag="P5293_06215"
     tRNA            109..182
                     /locus_tag="P5293_06215"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Ile,seq:gat)
     gene            203..276
                     /locus_tag="P5293_06220"
     tRNA            203..276
                     /locus_tag="P5293_06220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Ala,seq:tgc)
     gene            complement(300..644)
                     /locus_tag="P5293_06225"
     CDS             complement(300..644)
                     /locus_tag="P5293_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49532.1"
                     /translation="MIQNCLYGVSSQPVAIHVANLELLKDNKSFSIPKTNLVAAKVLK
                     KLPESQHMVLNRHLKHGLPPINLETISYMFTPLTKAFEDEIISTGFDMVICHLPYVCN
                     KEFTENEKMKLI"
     gene            complement(678..1484)
                     /locus_tag="P5293_06230"
     CDS             complement(678..1484)
                     /locus_tag="P5293_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRR49533.1"
                     /translation="MKSLLTKVLSTILLSIILISCGKVMITGRKQVLLFSQGEITSLS
                     NQAYSELKTKAQLSDNEHYNKMVNEVGIRLTKAVTDYCKQHGWESALEGLEWNFEVFK
                     DETVNAFCLPNGKIVFYEGILKYANTPDLVAVVMGHEIAHALAKHGNERMSQEVLVGA
                     VGQAVSTYLGTESQKTQAIFELAYGVGSQFGVLLPYSRKHEYEADRLGLIIMAMAGYD
                     INVAPKFWQTMSSIGGSKQPEFISTHPSDENRIKKIESVLPEAAKYYSKK"
     gene            complement(1531..2454)
                     /gene="nadA"
                     /locus_tag="P5293_06235"
     CDS             complement(1531..2454)
                     /gene="nadA"
                     /locus_tag="P5293_06235"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRR49534.1"
                     /translation="MEKLEVIEKIKKLKSEKSAVILAHYYTNPEIQDICDFIGDSLGL
                     SQEAGKTDAKIILFCGVNFMAETASIISPQKKVIVPDITAGCSLAESVTAEDLEEWKA
                     LNPDGIIISYVNTTADVKAHSDICCTSANALKVVESIPSNKKILFVPDRHLGKYISIK
                     SGRDMELWYGDCCVHDRFNSKVIIDMSTRYPEADILIHPESSCSGDPDILNMPNAYFY
                     STSGMVRHAAKSDKRQFIIATEKEIIHHMQKVIPGKEFIPINAQAICGHMKKATLEKA
                     LLALETEQPEIKVPEDIREKAWIPIRRMLEL"
     gene            complement(2461..3303)
                     /gene="nadC"
                     /locus_tag="P5293_06240"
     CDS             complement(2461..3303)
                     /gene="nadC"
                     /locus_tag="P5293_06240"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRR49535.1"
                     /translation="MEREDLIDKLIALAIEEDIYTGDITTNALIPQESRATAELTAKA
                     AGIVAGINVVPRVFKRLDSSVNWSPLIKEGGRVSKGDKIAKIEGSYRALLSAERTSLN
                     ILQRMSGIATMTSIYVKELEGTHTKLLDTRKTAPGMRILDKMAVKAGGAVNHRMGLYD
                     MALIKDNHIKVAGSISNAIKQVRATISAGIKIEVEVTTIIEVEEALKAGAEMIMLDNM
                     SNEMMTAAVKLIGKRAKTEASGNMTLARLKEVAATGVDYISVGALTHSVTALDISMNI
                     VPHK"
     gene            complement(3316..4893)
                     /gene="nadB"
                     /locus_tag="P5293_06245"
     CDS             complement(3316..4893)
                     /gene="nadB"
                     /locus_tag="P5293_06245"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRR49536.1"
                     /translation="MYYYDYIIIGSGLAGLYASYRASNYGKVALITKSNIRESNSYYA
                     QGGIAAVTDEEDSPAFHYEDTIVAGRGLCDHTAVNILVNEGPHRIMELVEDGMRFDML
                     GNNFALGLEGGHHMRRILHAGGDVTGMKITDFMINKVINDKNIDLFENRVAISLICED
                     GTCYGVRSWNSKKECEEIFVGKFSFLTSGGASAIYKRTTNPHTTTGDGVAITYESGCR
                     LADMEFIQFHPTALYTEQDKTFLISEAVRGEGAYLINTKGERFMPAIHELAELAPRDV
                     VARSIYLQILNQKEPYVYLSLKHLDPERIKTRFPNIFEKCCQLGIDMTDKIPVAPAAH
                     YMVGGVRTDCNGRTNVNNLYVCGELASTGIMGANRLASNSLLECLVFGYRAVEDTREA
                     YNNDANLAVQPTFSHIFYKNPANEIPYLDLKERVANIMNEKAGIIRNENLLTEGLEML
                     ENEKNKIGRDINEIYTHLSNNLITVAELIIKSAIYRKESRGGHYREDYPNEDPNFVKH
                     IIQQKGKPITTIPVDDK"
     gene            5123..6589
                     /locus_tag="P5293_06250"
     CDS             5123..6589
                     /locus_tag="P5293_06250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRR49537.1"
                     /translation="MSIRVNCFIPYQDEAQVKETVDGLRSSSMTDHIYLMTINPEAKP
                     LKGCEILYVESLKCVPAIRKIAERADGDFTLIYTKYSTLVMGLFALDRMVKIAEDSSA
                     AMVYADHYQIIDGKKSISPVITYQMGSLRDDFNFGSVILYRSSSLREAVLRMKTDYKF
                     AGLYDLRLKVSQKGKLVHINEYLYTEIEQDTRKSGEKIFDYVDPKNREVQIEMEAACT
                     EHLKEIGGYLNPDFKHIEFSKGDFEYEASVIIPVFNRIRTISDAISSVLRQKTTFKFN
                     LIVIDNHSTDGTYEAIEMFISDERLIHLVPDRDDLGIGGCWNVGVHHPKCGKFAVQLD
                     SDDIYSDENTLQKIVDSFYAQNCAMVVGTYMMTNFNMEMIAPGIIDHREWTPENGRNN
                     ALRINGFGAPRAFYTPVLREIKVPNTSYGEDYALGLNISREYQIGRIYDVLYHCRRWE
                     GNSDAALDIVRMNANNTYKDRIRTWELQARIAMNKREK"
BASE COUNT         1838 a         1287 c         1292 g         2189 t
ORIGIN      
        1 cgtaacaagg tagccgtacc ggaaggtgtg gctggaacac ctcctttttg gagcttaatt
       61 gactgaattg ttacactttg ttactcaaag ctatcattga ttttatatag tcccgtagct
      121 cagttggtta gagcactaca ctgataatgt aggggtcagc ggttcaactc cgctcgggac
      181 tacaaaaagt tcttaatttt ttggggaatt agctcagttg gctagagcac ctgctttgca
      241 agcagggggt caagggttcg actcccttat tctccacttt tgtcagcaaa gccatactct
      301 tatatcaatt tcattttttc attttctgtg aattctttat tgcagacgta agggagatgg
      361 caaatgacca tatcaaatcc tgtacttatt atttcatctt caaatgcttt tgtcagggga
      421 gtaaacatat aggatattgt ttcaagattg atgggaggta ggccgtgctt caaatgtcta
      481 tttaaaacca tatgttgaga ttccggtaat tttttcaaca cttttgcagc tactagattt
      541 gttttaggaa tagaaaaaga tttgttgtct ttaagaagtt caagatttgc cacatgaata
      601 gccacaggtt gactgctaac tccatacaga caattttgta tcaaatataa ctttcgttca
      661 aagttgtttt cattcagtca ttttttagaa taatactttg ccgcttcagg cagtactgac
      721 tctatctttt ttatacggtt ttcatctgag gggtgtgtac ttataaattc aggctgtttt
      781 gaaccaccaa ttgaactcat ggtctgccag aattttggag caacatttat gtcgtagccg
      841 gccatagcca ttattataag tcccaatcta tctgcttcgt attcgtgttt tctcgaatag
      901 ggcaggagaa caccaaattg tgacccaaca ccgtatgcaa gttcaaatat tgcttgtgtt
      961 ttttggcttt cagtgcctaa ataggtggaa acggcctgac caacagctcc cacaagcact
     1021 tcctgactca ttctttcatt tccatgcttt gccagggcat gggcaatttc atgtcccata
     1081 acaactgcaa caaggtcagg agtattagca tattttagta taccttcata gaatacaatt
     1141 ttgccgtttg ggaggcaaaa agcattcaca gtctcatcct taaatacttc aaagttccac
     1201 tcaagacctt caagagcact ttcccaacca tgttgtttac aatagtcggt aacagctttt
     1261 gtaagtctta tgccaacctc attgaccatt ttgttataat gttcattgtc cgacagctgt
     1321 gcttttgttt ttaactcact atatgcctgg ttgctgagtg atgttatctc tccctggcta
     1381 aataataaca cttgttttct tcctgtgatc attactttcc cacaggaaat caaaattatt
     1441 gataacagta tggtcgataa tactttagtc aacaaacttt tcatggcaat acaattagaa
     1501 tattatttta aaacaaatgg gcggaataac ttacagttca agcatccgcc tgattggtat
     1561 ccatgctttt tcgcgaatat cttcaggaac ttttatttca ggctgttctg tctcgagcgc
     1621 caaaagtgcc ttttcgagag tagctttctt catatggcca catatggctt gtgcatttat
     1681 cggaataaac tctttaccag gtattacttt ttgcatatga tggataatct ccttctcagt
     1741 ggctataata aactgccgct tgtcagattt tgctgcatgt ctgaccatgc cgcttgtaga
     1801 ataaaaatat gcattgggca tgttcaatat atcaggatct cctgagcaac tgctctccgg
     1861 atggataaga atatctgctt caggatagcg ggtagacata tctataatca ctttggagtt
     1921 aaaacgatca tggacacagc agtctccata ccaaagttcc atatctctgc cacttttgat
     1981 actgatatat tttcctaaat gcctgtcagg cacaaagaga attttcttgt tagaagggat
     2041 tgattccacg actttgaggg cattagcaga tgtacagcaa atatcagaat gtgctttaac
     2101 atctgcagtg gtattgacat atgaaattat tattccgtcg ggatttagtg ccttccactc
     2161 ttccaaatct tctgctgtaa cactttctgc aagtgaacat cctgcagtaa tatcgggtac
     2221 tataactttc ttttgaggag agataataga agctgtttcg gccataaagt taactccgca
     2281 aaaaagaata atctttgcat ccgttttacc agcttcttgt gacagtccaa gagaatctcc
     2341 gataaagtca catatgtctt gaatttcagg atttgtataa tagtgagcga gaattaccgc
     2401 actcttttcg gattttagtt ttttaatttt ctcaataacc tctaatttct ccatgggcat
     2461 ctatttgtgt ggtacaatgt tcatgctaat gtccaaagca gtgacagaat gtgtcagtgc
     2521 accaacgctg atataatcca ctcctgtggc ggcaacctct ttgagtcttg ctaatgtcat
     2581 gtttccggaa gcttctgttt tggctctttt gcctataagc ttgacagccg cagtcatcat
     2641 ttcgttactc atgttatcca acattatcat ctcagctccg gctttgagtg cttcttcaac
     2701 ctcgatgatt gttgtgactt ccacttcgat tttgattcca gcagaaatag ttgctctaac
     2761 ctgtttaatc gcgttagata tgctacctgc aacttttata tgattgtctt ttataagtgc
     2821 catgtcgtag agtcccatgc ggtgattaac tgcacctccg gccttaacag ccatcttatc
     2881 caaaatacgc attcccgggg ctgttttacg cgtatcgaga agtttggtat gcgtgccttc
     2941 gagctctttg acataaattg atgtcatagt ggcaatgccg ctcattcttt ggaggatgtt
     3001 aagtgatgtg cgctctgctg atagcaaagc acgataactt ccttcaattt ttgcaatttt
     3061 atctcctttt gaaactcttc cgccttcttt tattaacggg gaccaattga cagatgagtc
     3121 gagacgtttg aaaactcggg gaacaacatt aattcccgca actattcccg cagcttttgc
     3181 ggtcaattct gctgtagcgc gagattcctg aggaatcagg gcatttgtgg taatatcgcc
     3241 tgtatatata tcctcttcta ttgcgagggc tatcagctta tcaataagat cttctctctc
     3301 catgtgattt tggcattatt tgtcatcaac aggtatggtt gtgattggtt ttcctttttg
     3361 ttggatgatg tgtttgacaa aatttggatc ctcatttgga taatcctctc tatagtgccc
     3421 acctctgctc tctttacgat atatggcaga ttttattatg agttcggcta cggttataag
     3481 gttattactc agatgggtat atatttcatt gatatccctg ccaattttat ttttttcatt
     3541 ttcgagcatt tcgagacctt ctgtaagtaa attctcgttt ctgattatac cagccttttc
     3601 gttcattata ttggcaactc tctctttgag gtcaagatag ggaatctcat ttgcgggatt
     3661 tttgtagaaa atatgagaaa aggttggttg aactgccaaa ttagcatcat tattatatgc
     3721 ttcacgagta tcttcaacag ctctgtaccc aaacaccaga cattcgagca gtgaattgga
     3781 tgcgagacgg ttggctccca taatgccggt agaggcgagc tctccgcaga cataaagatt
     3841 attgacattg gttctgccgt tgcagtctgt tctaacaccg ccaaccatat aatgtgctgc
     3901 tggtgcaaca ggtattttgt ctgtcatgtc tatgccgagc tggcaacatt tttcaaatat
     3961 attcggaaag cgagttttaa tgcgttcagg atccaggtgc ttaagagaaa ggtagacata
     4021 tggctccttc tggtttaaga tctgaagata aatggatctt gcgacaacat cccttggagc
     4081 aagctctgca agttcgtgga tagcaggcat aaagcgttcc ccttttgtgt ttatgagata
     4141 tgctccttca cctctgacag cttcacttat taaaaaagtc ttgtcctgct ctgtgtaaag
     4201 tgctgtcgga tggaactgaa taaattccat gtctgcaagc ctgcatcctg attcatatgt
     4261 tatagcaact ccatcgccgg tggttgtgtg tgggtttgta gttcgtttgt atattgctga
     4321 tgccccacca gaagttaaaa aagaaaattt tccaacaaat atctcttcgc actctttttt
     4381 agaattccaa cttctgacac catagcaggt gccatcttcg catataagag aaatagccac
     4441 tctgttctca aaaagatcta tgtttttgtc attaattacc ttatttatca taaaatcggt
     4501 aatcttcata cctgtgacat cacctcctgc atgcagaatt cttctcatgt gatgtccacc
     4561 ttcgagacca agggcaaaat tattgccaag catgtcgaac ctcattccat cttcgacaag
     4621 ttccattatc ctgtgtggtc cttcattgac aaggatattt acagcagtat ggtcacacaa
     4681 tccacgacca gctacaattg tgtcttcata atgaaatgcc ggactgtctt cctcatcagt
     4741 gacggcagca atacctccct gtgcataata ggagttgctt tctcttatat tcgactttgt
     4801 gatcaaagcc actttcccat aattagatgc tctgtatgag gcatataagc cggcaagtcc
     4861 acttccaata atgatatagt cgtaatagta cattccctat ctctatgagt tgcgaaatta
     4921 ctcttttttc tttaaaggtg cttcacttat gataaacaat cttaatatta cttggttact
     4981 aatggtttga aatgttaact tgttatactt tgtttataag gtttaataat ctattataca
     5041 gaatattaaa ttgtttactt tgtattttgc atcaaatatt attaccttag caagcatatt
     5101 ttgaaactta atacataatt gtatgtcaat cagggttaat tgcttcattc catatcaaga
     5161 tgaagctcaa gtaaaagaga ctgttgatgg actcagaagt agttctatga cagaccatat
     5221 ttatcttatg actatcaatc cggaagcaaa accgttgaaa ggatgtgaaa tattatatgt
     5281 cgaatctttg aaatgtgtac cggccattag gaaaattgca gaaagagcag atggcgattt
     5341 tacgcttatc tatactaagt attcaacttt ggttatgggg ttgtttgctt tggatagaat
     5401 ggtaaaaatt gcagaggaca gttccgctgc gatggtttat gcggatcatt accaaataat
     5461 tgatggcaaa aagagcatca gtccggttat aacatatcag atgggaagtc ttagagatga
     5521 ttttaatttc ggttctgtta ttctttacag gtcttcatct ctgagagagg cagttttgag
     5581 aatgaaaacc gattataaat ttgctggtct gtacgacctg cgtctcaaag tgtctcaaaa
     5641 gggaaaactt gttcatatta atgaatatct ttatactgag atagagcagg atactcgcaa
     5701 gtcaggtgaa aaaatatttg attatgttga cccaaaaaac agggaggtcc aaatagagat
     5761 ggaagctgcc tgtacagagc atttgaagga aataggcggt taccttaacc cagattttaa
     5821 gcatattgaa ttttctaagg gcgactttga atatgaagca tccgtaataa tccctgtctt
     5881 taacaggatt agaactattt ctgacgccat ttcctctgta ttaaggcaaa aaacaacatt
     5941 taaatttaat ctgattgtta ttgataatca ctctacagat ggtacttatg aagcaatcga
     6001 gatgttcatc agtgatgaga ggctgattca tctggtgcct gacagagatg atttaggtat
     6061 aggtggttgc tggaatgtcg gtgtacatca tcctaaatgt ggcaagtttg ctgttcagct
     6121 tgacagcgat gacatatatt ctgacgaaaa tactcttcaa aaaattgtgg actcttttta
     6181 tgctcaaaac tgcgcaatgg ttgtaggtac ctacatgatg acgaatttca atatggaaat
     6241 gattgctcca ggcataattg accatagaga gtggactcct gaaaatggca ggaacaatgc
     6301 tctgcgcatt aatggttttg gagcaccgag agctttttat acccctgttt tgagagaaat
     6361 caaggttccc aacacgagtt acggagagga ttatgcttta ggcttaaata tttctcgtga
     6421 atatcagata ggcagaatat atgacgttct ttatcattgc cgacgttggg aaggaaactc
     6481 tgatgctgcc ctcgatatag taagaatgaa cgcaaacaac acctataagg ataggataag
     6541 aacgtgggag cttcaggcaa gaattgctat gaacaaaaga gagaaatagt tagatgtcag
     6601 agttaa
//
LOCUS       DAOTQL010000096         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_9632_length_6601_cov_4.507791, whole genome shotgun sequence.
ACCESSION   DAOTQL010000096 DAOTQL010000000
VERSION     DAOTQL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9632_length_6601_cov_4.507791"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="P5293_06255"
     CDS             complement(<1..911)
                     /locus_tag="P5293_06255"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRR49538.1"
                     /translation="MAQIVNDISRTFNEYLLIPGLTTKQCTPDKISLKTPLTKFKKDS
                     ESDLYLNIPFVSAIMQSVSDDKMAIALARNGGLSFIFCSQPIKEQADMVRRVKKYKAG
                     FVVSDANLTPEHTLQDVINLKQAKGFSTIGITHDGTSNGILLGLVTSRDYRPGIDDPG
                     KKIKDFMTPFSKLITGKYGITLDEANNIIWENKINALPIIDKQQHLKYFVFRKDYENH
                     KNNPYELLDTHKRLIVGAGINTRDYKERIPSLIDAGADVLCIDSSDGYSEWQKETIDF
                     IRHKYGEKVKVGAGNVVDKDGFYYLAEA"
     gene            1004..2356
                     /locus_tag="P5293_06260"
     CDS             1004..2356
                     /locus_tag="P5293_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRR49539.1"
                     /translation="MNNSNSAPTALGTERIWKLLLQFAIPSVIAMTASSLYNIIDSIF
                     IGQGVGPLAISGLAISFPLMNLSAAFGSLVGAGGAALMSLRLGQKDYSSANLILGNAF
                     TLNLVIGTIYAIVVLIFLDPILYFFGASDATIPYARDYMEIISLGNVITHLYFGQNAL
                     LRASGFPAKSMYATIGSVLINIILAPIFIYVFKWGIRGAALATIIAQASMLAWQMLIF
                     CNKSNFIHFHKGVFKLRKKIVKDALSIGLAPFLMNSVSCVVVIVINQGLIRYGGDLQV
                     GAYGIINRISFFFAMIVAGLNMGMQPVAGYNYGARQLARVNKALKLTILLATSVMSIG
                     FIVGEFFPRAVASLFTSDEGLISLVVQGMRIVLIFFPIVGFQMVASNFFQSIGKPGKA
                     IFMSLSRQVVFLLPALFILPNIFGVKGIWYSIPLADLLASMITAYLLFHQLKKSKLNL
                     "
     gene            2358..2960
                     /locus_tag="P5293_06265"
     CDS             2358..2960
                     /locus_tag="P5293_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRR49540.1"
                     /translation="MNEYCVINIGRQLGSGGHEIGERLAELFGFSFYDKELIRLASME
                     SGLCSEVFERADERVRYSVFDGLFGVTDNYLSNETLFQIQSDVIRKLANEGSCVFVGR
                     CADYVLKDHPRAINLFITANLEERVKRVMIQQHLSLAEARSHIEKTDKRRATYYNFYS
                     NKVWGVADSYDLCVNTSVLGIDETVKFLKIFIEKKFFPDR"
     gene            complement(2957..4489)
                     /locus_tag="P5293_06270"
     CDS             complement(2957..4489)
                     /locus_tag="P5293_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRR49541.1"
                     /translation="MNTEEIYYQPRDYKKIELWKNVSEKDWNDPLWQQKNSIKTVEQL
                     SKVITLTEYQKKEIERTVSTLISQGKEPLRITPYYASLMQEDPFHPIMPKGEKPEKRL
                     DPIFWQSVPTPANLLFPDTGIEGAMAEGSRSYGAAYQRYPNRVALFVAENTSCASYCV
                     HCQRAKSLDTSVDVNKTEIDKGLFYIGYNKNINEVLVTGGDALMISRSRLKYIMEELS
                     KIPHLRTIRIATRVPVVLPMAITDDILNLIEESANKYNTGPEKYVYFVTHINHYHEIT
                     EDLAKAVKLILKHGFTVRNQTVLLNHVNDYYKTLAETFRRMFWIGVHPYYILQCHKEK
                     GIVHFITPIQVGMIYMQNLQGWVSGITVPRYAANIEGGGGKVVLMPSGHDTLNLGHKI
                     DEKISESFATIYTWDDKKILRYEALGRTTYEEFKAGVEVMDRFIGRKGVFLPKLIIVD
                     ENGNHIETTNRTKLPTLEKIKKAELLEYEVFKDDMPLTNPALIQQQLDESFKQSKFMQ
                     IN"
     gene            complement(4539..5549)
                     /gene="amrS"
                     /locus_tag="P5293_06275"
     CDS             complement(4539..5549)
                     /gene="amrS"
                     /locus_tag="P5293_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRR49542.1"
                     /translation="MEITAKYQRKGSDSKVQCILCPHNCHLEDGQRGICRVRINMQGE
                     LISIAYGNPCSISIDPIEKKPLYHFLPGTDIFSLAIEGCNLRCLNCQNWTISQATPDT
                     KEQYILLPEEVISESIKKRSKSIAFTYSEPTVFYEYMLDTFRIAKENGLRTVAVSNGF
                     INKEPLIELCNWLDAANIDLKCFDDSLSKKLTGGAMKPVLKTLGILRDKGIWLEITNL
                     IVPGWSDNMQIIKRMCEWLYDNGFSDTPLHFSRFFPNYKLANLHPTAEKTMIEAKNTA
                     LKCGLKYIYLGNIYIPECGDTICPTCHTTVVKREGYIVRSYVTPEGTCPKCGQKIPGI
                     WK"
     gene            5640..>6601
                     /locus_tag="P5293_06280"
     CDS             5640..>6601
                     /locus_tag="P5293_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRR49543.1"
                     /translation="MSGFFGCVSKEDCVSDVFYGTDYHSHLGTKRAGVAYFNKSQGFQ
                     RAIHSLEDGYFRNKFENDIKGFAGKSGIGVISDTEAQPIVVNSHLGRFAVATVSKIVN
                     SDEIEDYFLKKHHTFSETSQGTVNPTEMIAMLIAEGEDFISGIRNVQERVKGSCSMLV
                     LTENGILAARDKLGRTPVIIGKKDGAYAAAFETCAFSNLGYEVERFLGPCEVVRITAD
                     GCEQLSKPGERMQVCSFLWVYYGYPPSYYEGINVDECRYRCGAALARRDCVKADFVAG
                     IPDSGIGHAIGYSHVSGIPLKRPYAKYTPTWPRSFMPQSQNMRE"
BASE COUNT         1605 a         1114 c         1524 g         2358 t
ORIGIN      
        1 gcttctgcga gataataaaa tccgtctttg tcaactacat tgcctgctcc gactttgacc
       61 ttttcaccat atttgtgtcg gataaaatct atggtttcct tttgccattc tgaatatccg
      121 tcggatgagt ctatgcagag tacatctgca ccggcgtcga tgagtgaagg gatgcgttct
      181 ttatagtctc ttgtattaat cccggctcct actatcaatc ttttatgtgt gtcgagcagt
      241 tcgtatggat tgtttttgtg gttttcgtag tcttttctga aaacaaagta tttgagatgt
      301 tgttgtttat ctataattgg cagtgcgttt atcttgtttt cccagattat gttgttggct
      361 tcgtcgagtg taatgccgta tttacccgta atcagtttgg aaaatggtgt catgaaatct
      421 ttgatttttt tgccggggtc atctattccc ggtctgtaat cacggctggt tacaagtcca
      481 agcaatatgc cgttggatgt gccgtcgtgg gttatgccta ttgttgaaaa tcccttggct
      541 tgtttgagat ttatgacatc ctgaagtgtg tgttcgggag tgaggttggc atcgctgact
      601 acaaatcctg ccttgtattt tttcactctg cggaccatat cagcctgttc ttttattggc
      661 tgggagcaaa atataaaaga taaccctccg tttcgggcaa gggctatggc cattttgtca
      721 tctgatactg actgcattat tgcagacaca aacggaatgt tcaagtacaa gtcggattct
      781 gaatcttttt tgaattttgt caatggtgtc ttgagactta ttttgtctgg agtgcattgt
      841 tttgttgtca aaccaggaat caaaaggtat tcgttgaatg ttctggagat atcgtttact
      901 atctgtgcca tggtaaatcg tgttatagat ttacaaagtt aaggaaattt aacaattttc
      961 tgtctatttt catttttagt aactttgccg atacatgaga agtatgaata atagtaatag
     1021 tgcgcctact gcactgggta ctgagaggat atggaaattg ctgctgcagt ttgcaattcc
     1081 ttctgtgata gcgatgacag catcttcgct ttataatatt attgacagta tttttatcgg
     1141 tcagggggta ggtccacttg ctatttctgg tttggctatc tcttttcctc tgatgaattt
     1201 gagtgctgca tttggatctc tggtaggtgc cggaggtgca gcgctgatgt ctttgaggct
     1261 tggccagaag gactattctt ctgctaatct tattttgggg aatgctttta cgttgaatct
     1321 tgtgataggg actatttatg ccattgtcgt gttgatattt ttagacccta ttctctattt
     1381 ttttggagcg agtgatgcta caattccata tgcgagggat tacatggaga taatctctct
     1441 gggcaatgtg ataactcatt tatattttgg ccagaatgct ctgttgcggg cttctggttt
     1501 tcctgcaaaa tcgatgtatg caacgattgg ttcagtattg attaatatta ttcttgcacc
     1561 gatttttatt tatgttttca aatggggtat tcgtggagca gctcttgcta ctattattgc
     1621 gcaggcctcc atgcttgcct ggcagatgtt gatcttttgt aataagagta attttattca
     1681 ttttcataag ggggtattta agttgcgcaa aaagatagtt aaggatgctc tttctattgg
     1741 tcttgctccg tttcttatga attcggtttc ttgtgtggtg gtgattgtga taaatcaggg
     1801 tttgataaga tatgggggtg atttgcaggt gggtgcttac ggtattatca acaggatatc
     1861 gttttttttt gctatgattg ttgcgggtct taatatgggt atgcagcctg tggccggtta
     1921 taattatggt gccagacagt tagcaagggt gaacaaggct ttgaaattga caatattgct
     1981 tgctactagt gtgatgagta taggttttat tgtaggcgag tttttcccga gagctgttgc
     2041 ttcgctcttt acaagtgatg aagggttgat atcattggta gtacagggta tgagaatcgt
     2101 tttaatattc tttcctatag tgggttttca aatggtggct tctaatttct ttcagagtat
     2161 tggtaagcca gggaaggcaa ttttcatgtc tttatcacga caggttgtct ttttgcttcc
     2221 cgccctgttt atattgccga atatatttgg cgtaaaaggt atatggtaca gtattccgct
     2281 agcggatttg ttggctagta tgatcacggc atatttactt ttccatcaat taaaaaaatc
     2341 aaaactcaat ttgtaaaatg aatgagtatt gtgtaattaa catcggtcgt caacttggta
     2401 gcggcggcca tgagattgga gaacggcttg ctgagctgtt cggattttcc ttttatgaca
     2461 aggagcttat ccgcctggct tctatggaaa gtggtttgtg cagcgaggtt tttgaaaggg
     2521 ccgacgagag agtcaggtac tctgtttttg acgggctgtt tggggtgact gacaattacc
     2581 tgagcaatga gactcttttt cagattcaga gtgatgttat aaggaaactg gctaatgagg
     2641 gttcttgtgt cttcgttgga aggtgtgccg attatgtgtt gaaggatcat ccaagggcta
     2701 ttaatttgtt tataactgcc aatttggagg agagagtaaa aagagtaatg attcagcaac
     2761 atctttctct tgccgaggca cgttctcata ttgagaagac cgacaagagg agggcaactt
     2821 actataattt ttacagtaat aaagtgtggg gagttgctga ttcttacgac ttatgcgtca
     2881 acacctccgt tcttgggatt gatgaaacgg ttaagtttct gaagattttt attgaaaaaa
     2941 agtttttccc tgaccgctaa ttgatctgca taaattttga ctgcttgaaa gattcatcaa
     3001 gctgttgctg aatcaatgcc ggatttgtaa gtggcatgtc atctttgaat acttcatatt
     3061 caaggagttc tgctttttta atcttttcaa gggtaggaag ttttgttctg ttggtggttt
     3121 ctatatggtt gccattttcg tctactataa tgagtttggg gagaaagaca ccttttctgc
     3181 caatgaatct gtccattact tcaacacctg ctttgaattc ttcatatgtg gttcttccaa
     3241 gagcctcgta tcttaagatt tttttgtcat cccaggtata aattgtggca aagctttcgg
     3301 atattttttc atcaatttta tgtcccaaat tgagagtatc atgtcctgat ggcataagaa
     3361 ctaccttgcc tcctccacct tcgatgtttg cagcatacct gggtactgtt attccagata
     3421 cccagccttg aaggttttgc atatagatca ttcctacttg aattggggtt ataaagtgaa
     3481 ctatcccctt ttccttatga cactgaagta tatagtaagg gtgaactcca atccagaaca
     3541 ttcttctgaa agtttcggca agagtcttgt aataatcatt gacgtgatta agaagtacag
     3601 tctgatttct gactgtaaat ccgtgtttca aaattaattt gactgctttt gccaaatctt
     3661 cggttatctc gtgatagtga ttgatgtgtg ttacgaaata aacatatttt tcagggcctg
     3721 tattatattt atttgctgac tcttctataa gattgagtat gtcgtctgtt atagccatgg
     3781 ggaggacaac tggcactctt gtggcgattc ttatagttct tagatgtgga attttgctta
     3841 actcttccat aatgtatttt aatctggagc gactgatcat cagagcatct cctcctgtta
     3901 cgagaacttc gttgatgttt ttgttgtatc ctatgtaaaa cagtccttta tcaatttctg
     3961 ttttgttgac gtcaacagaa gtatccaacg actttgctcg ttggcaatga acacagtatg
     4021 atgcacaact ggtattttct gcaacaaaaa gagcaactct gttagggtat ctttgatatg
     4081 cagcgccgta agatctggaa ccttcagcca ttgctccttc tattcctgta tctggaaata
     4141 ataaatttgc cggggttggc acactttgcc aaaatattgg atctaatcgc ttttcaggct
     4201 tttctccttt cggcattata ggatggaagg gatcttcctg cataagcgac gcataatatg
     4261 gtgtaattct gagcggttct tttccttggg atatgagagt ggacacagtt ctttcaatct
     4321 cctttttttg atattcagtt aatgtgatta ccttagataa ttgttcaact gtttttatcg
     4381 agttcttttg ttgccaaagc ggatcattcc aatctttttc gctgacattt ttccataact
     4441 caatcttttt gtaatccctg ggttgataat aaatttcttc tgtattcata tattaatttt
     4501 gggacacaat ttaactaaaa ttatgttaaa tagaaaaatt atttccagat cccggggatt
     4561 ttttgaccgc attttgggca tgttccttct ggggtcacat aacttctgac gatgtaacct
     4621 tctcgtttta ctactgttgt atggcaggtc ggacaaatgg tgtctccgca ttcagggata
     4681 tagatatttc ccaggtatat atatttcaat ccgcatttga gggcggtatt tttggcttct
     4741 atcattgttt tttctgctgt ggggtgcaga ttagcaagtt tgtagttagg gaaaaatctg
     4801 ctgaagtgaa gaggtgtgtc gctgaatccg ttgtcataga gccattcaca cattctcttg
     4861 ataatttgca tgttgtcgga ccacccgggt actattaggt ttgtgatttc gagccatata
     4921 cctttgtctc tcaatattcc aagggttttg aggacaggtt tcattgctcc gcctgttagt
     4981 tttttgctga gagagtcgtc aaagcattta agatcgatat tggctgcatc gagccagtta
     5041 catagttcaa ttagtggctc tttattgatg aatccgtttg acacagctac agttctgagc
     5101 ccgttttctt ttgcaattct gaatgtgtcg agcatgtact catagaagac agttggctcg
     5161 ctgtatgtga atgcgatgct tttacttctt ttttttattg attcggagat tacctcttca
     5221 ggaagcagga tgtattgttc tttggtgtcg ggggttgctt gggagattgt ccaattttgg
     5281 cagttgagac atctcagatt gcaaccttct attgcaagtg agaaaatatc tgtgcctgga
     5341 aggaaatggt aaagtggttt tttctctatc gggtctatgg agatggagca agggttgccg
     5401 taggcaattg aaataagctc tccctgcata ttaattctaa ctctgcatat tcctctctgg
     5461 ccatcttcaa gatgacagtt gtgtgggcag aggatacact gaactttgct atcagagcct
     5521 tttcgttgat atttggctgt aatttccata acggggcgta aatttaatat cttttttggg
     5581 ctttttatca ttttcgggta catttgtaaa ttattccaat atctataaat ctgatttaaa
     5641 tgagtggttt ttttgggtgc gtttccaaag aagattgtgt gagtgatgtt ttttatggta
     5701 ctgattatca ctcacatttg gggacgaaaa gggcaggggt tgcctatttc aataaaagtc
     5761 agggatttca aagagcgatt cacagtcttg aggatggtta cttcagaaat aagtttgaaa
     5821 atgatattaa gggttttgcc ggtaagagcg gcattggggt aatcagtgat accgaagccc
     5881 aacccattgt cgtgaattcg catttgggac gttttgctgt ggctacggtc agcaaaattg
     5941 tcaattcaga tgaaatagag gactattttc tcaaaaaaca tcatactttt tctgaaacga
     6001 gtcaggggac tgtcaatcct acggagatga tagccatgtt aattgctgag ggagaagatt
     6061 ttatatccgg gatccgaaat gtgcaggaga gggtaaaggg ttcttgctct atgcttgttc
     6121 ttacggaaaa tggtatttta gctgcaaggg acaaacttgg caggactcct gtgattattg
     6181 gcaaaaagga tggtgcttat gctgctgcat ttgaaacttg tgccttttcc aatttgggct
     6241 atgaagtgga gaggtttttg gggccttgtg aggttgtgag aattactgca gatgggtgtg
     6301 aacagttgag taagcccgga gaacgaatgc aggtctgttc ttttctgtgg gtatattacg
     6361 gttatcctcc ttcttattat gagggtatta acgtggatga gtgtcgttat agatgtggag
     6421 ctgctcttgc aaggagggac tgtgtaaaag ccgattttgt ggcggggatt ccggattcag
     6481 gtattggaca tgccataggt tacagtcatg tcagcggtat tcctcttaag aggccttatg
     6541 ctaaatatac tcctacctgg ccgagaagtt ttatgcctca aagtcagaat atgagggagt
     6601 t
//
LOCUS       DAOTQL010000097         6511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_9787_length_6511_cov_5.136307, whole genome shotgun sequence.
ACCESSION   DAOTQL010000097 DAOTQL010000000
VERSION     DAOTQL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9787_length_6511_cov_5.136307"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="P5293_06285"
     CDS             <1..372
                     /locus_tag="P5293_06285"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRR49544.1"
                     /translation="HDISTLREWWETLGKEKEIYYHSYLHFTHKAPKSCKIAICQEII
                     RQHLESSSMMAILPIQDWMSLNKNTRRDNPKEERINDPSDPNNYWQFRIHLNMEKILS
                     NKTFLNKIKQMVNDSGRIAKK"
     gene            complement(382..1401)
                     /locus_tag="P5293_06290"
     CDS             complement(382..1401)
                     /locus_tag="P5293_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRR49545.1"
                     /translation="MKRFEFIFLLLCFILIKNSSIAQNVSTVKNAGNWAIALHGGAGS
                     LSPNLSPEKIKLLEEHMIKALSLGESVLRDGGKSLDAVQVVVTYLEDCPLFNAGKGAV
                     TNLQGIHELDAAIMDGSNLKAGAVAGVKDIKNPIKAARLVMDSTKHVLLIGEGASEFA
                     KSQGLEIVDNSYFSTDERIEQNQKIKEGSEKPYPGGTVGCVALDVNGNLAAATSTGGM
                     SGKKWGRVGDVPVIGAGTYANNQTVAVSGTGHGELWIRRVVAFDISALMDYKGYSLEE
                     AANEVIFKKISPMGGSGGGIIAVDKNGNISIVFNTGTMNRAWSKSDGSCGVGVLKGDD
                     RNFKR"
     gene            complement(1518..2759)
                     /locus_tag="P5293_06295"
     CDS             complement(1518..2759)
                     /locus_tag="P5293_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HRR49546.1"
                     /translation="MKKRFHILLLMVVALFTSCDKENSDDSGEPYFIFKDATESTTPT
                     GYDVAFTGNSSGLPFVIRSNRPWKIVANEQDTWARFFPDEGEDDGIVKLIVSENKTFV
                     NRQMSFKFIVDGEEQPVLFTVTQAKAIPFLTISDIDGKRISQLEQTFTINVKANVEYT
                     YTSDVSWLTFVSKSVGATSTDLSFSVTANDAIDSREGNILFSCSQFPELNATLKVIQE
                     GKGEGTVVLFDDFSWAKYSTTVFYSTTDAKRYDAWTDEERAKGWSTTPNVFSKNEPLL
                     YACYGFLKIGKTTYGGDLITPALNALPAGSHNLLVKFKAVPYMTASGTKDDNYLKIII
                     VGPGTASVSQFVIDNWPNYTTDPTCTAIWADPSTERSFTITGATSETKIKFLGGDYNL
                     SGVGAGKNRIFLDDIKVLIPN"
     gene            complement(2774..4198)
                     /locus_tag="P5293_06300"
     CDS             complement(2774..4198)
                     /locus_tag="P5293_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcineurin-like phosphoesterase family protein"
                     /protein_id="HRR49547.1"
                     /translation="MNRFLGIVLFLTFSLSLFGRDISGVVDSDGKGLKNVVVTDGFNF
                     TQTNENGVFKLNVSTESEFVYIFIPSGYRAVAKEGISQFFKRIDQQSDNLHFELNKIP
                     DCNKYVLIAVGDPQTKTLEQYNIFEQTVISDLKSTIDGYIKKNVIPVTVYLGDVVWDN
                     MKLFDNHKQGISKLGTPVYSAIGNHDYQKELIGDTNCGKAFGNCFGPAYYGFVLGKNY
                     VIVLDNIIYNTNKQYIEDVSQSQIEWLKGYLKFVPKDAEIFIVMHAPVNKPWIDNYKL
                     SLGHQQIMNILSGYKLSFITGHTHINNNLDIIPGVMEHNVASSCGAFWKSFYCMDGTP
                     SGYQIFEFNENECSWYFKTIGKDRSYQMELYKPGSFSNMSDAVVAKVWNWDPLWKVEW
                     FGNGKYMGEMKQFKSTDPKYISDMEKLIEKGKLTRQEFEKANYLKPRLSQFFFAAFPL
                     KGIREIKVVVTDRFGNTYNDIILN"
     gene            complement(4220..5791)
                     /locus_tag="P5293_06305"
     CDS             complement(4220..5791)
                     /locus_tag="P5293_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRR49548.1"
                     /translation="MKRIFIISGIALLGVMSSCTKDFEETNTNPNKAIVGMIQASGMF
                     EPLLYNSANNWLNDTWYYNNELVQFTAHTGGVTRREHQYFLEESRDWGGFWNFYANYG
                     NNTDHMHELAVKQGDKSLEAIALTFKVLFFSNMTDMFGSVPYEEAFSGRKIGGTLTPK
                     FNTQKDIYQFLFEDLENANDIYAQNPVFLNPSMDGMYGGNMTKWRKFNNSLYLRLLCR
                     VSGRSEMNVGAKMTEIITNPSKYPIFTSNADNATVKFSGVDPYINTFGTTTESDFTKS
                     GRKLTRQLISMTVITENGNQIYVDPRLPIIGKKNTVASDNPYNIWIGTKSGCTDLERA
                     TVDRGTSWLNYKVFCRKDATATFMDYAEIQFILAEAALKNLISGGASAAKNYYEAGVR
                     ASMEKWSALGAYSETPTSISSAEINTFLSSKLGSWDLASNKEELIGNQKYLALFWIGM
                     EAYHEYRRTGYPVLTIGEGTVYNDNVFPTRFGYPPTTVSTNSANVAIALEQMGGPNNM
                     KTPVWWSKQAIQSGL"
     gene            complement(5810..>6511)
                     /locus_tag="P5293_06310"
     CDS             complement(5810..>6511)
                     /locus_tag="P5293_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRR49549.1"
                     /translation="GLKRAPEGSYYVDANGNNIDCSGQVLIDASTGLPSLTSSADVCL
                     GNVNPDWKGGLNTSIRYKRITLNMAFTYQWGGHRFSVTNGILSYQGKLKNSLDGRYDG
                     KVAEGVNVISTNEDGSYVCAQNNTITSSIYTYYQALTLDRYNGEANTFDTSFLKFKEA
                     RLEYSLPETLCKKVGFLQGASIAIFATNIFSWDNWPQFDPEAGMLQGTNVFNGIETGA
                     FPMTRSYGVNLRLQF"
BASE COUNT         2070 a         1331 c         1005 g         2105 t
ORIGIN      
        1 catgatatca gcactctaag agagtggtgg gaaactcttg gtaaagaaaa agagatttat
       61 tatcacagtt atttacattt tacgcataaa gctcctaaat catgtaaaat agcaatatgt
      121 caggaaatta tccgccaaca tcttgaatcc tcttccatga tggcaatttt acccattcag
      181 gactggatgt ctctaaataa gaatacaaga agagataatc ccaaagagga acgcatcaac
      241 gatccgtcag atccaaataa ttattggcaa ttccgaattc atttaaatat ggaaaagata
      301 ctgtcaaata aaacttttct taataaaata aaacaaatgg tcaatgattc tggacggata
      361 gcaaaaaaat aatattttag tttatctctt gaaatttcta tcgtcacctt taagtactcc
      421 tacaccgcat gatccgtcag atttagacca tgctctattc atcgtgccag tattgaacac
      481 aatggatata ttaccatttt tatctacagc tattattcct ccgcccgaac ctcccattgg
      541 ggatattttc ttaaatatta cttcgttggc tgcttcttcg agtgagtaac ctttataatc
      601 cataagtgca gaaatgtcaa aagcaaccac tctccgtatc cacaactctc catgtcccgt
      661 acctgacaca gccacggttt gattgtttgc atatgttccg gctccaataa caggtacatc
      721 tcctaccctg ccccattttt tacctgacat tcctccagtg gaagtagctg ctgcaagatt
      781 tccattaaca tcaagagcaa cacaaccgac agtaccacca gggtatggct tctcagatcc
      841 ctcttttatt ttttgattct gttcaatcct ctcgtctgtg gaaaaatatg aattatcaac
      901 gatctctaaa ccttgacttt tagcaaactc cgatgcacct tctcctataa gaagtacatg
      961 ttttgtgctg tccataacaa gtcttgcagc tttaatcgga ttttttatat cttttacccc
     1021 ggcaactgca ccagctttta aattggaacc atccattatc gcagcatcga gctcgtgaat
     1081 gccttgtaaa tttgtaacag caccttttcc tgcattaaac agggggcaat cttcaagata
     1141 agtaacgaca acttgaacag catcaagact ctttcctcca tctcttagaa cactttcacc
     1201 aagtgagaga gctttaatca tatgctcttc caataacttg attttttctg gagaaagatt
     1261 tggtgaaagt gagcctgctc caccgtggag cgctattgcc caattccctg catttttaac
     1321 tgtagagaca ttttgcgcta tactgctatt ttttattaaa ataaagcaaa gcaatagaaa
     1381 aataaattca aatcttttca taaataatta ttgttcagtt taatcaattc aaaattataa
     1441 aaaaaagaat aatagaaaaa gggtgaatga aacttaattt caaacaccct tttcccgcct
     1501 caataattac taacaaacta attaggaata agaactttaa tgtcatctaa gaaaatccta
     1561 ttcttaccag ctcctactcc cgaaagatta taatcacccc ccaaaaactt tatcttagtt
     1621 tcagaggtag ctcccgtaat tgtaaatgac ctttctgtac taggatcagc ccatatggcg
     1681 gtgcaagtgg gatcagttgt ataatttggc cagttatcta ttacaaactg agaaacactt
     1741 gcagttcccg gacctacaat aattattttt aaataattat catctttggt cccgctcgct
     1801 gtcatatatg gtacagcctt aaacttgaca agaagattat ggctgcctgc tggaagggca
     1861 tttaatgcag gagtgattag atcacctcca taagttgtct tacctatctt caagaagcca
     1921 taacatgcat acagaagagg ttcattcttt gaaaagacat taggagtagt agaccatcct
     1981 ttagctctct cttcatcagt ccaggcatca tatctcttag catcagttgt actgtaaaaa
     2041 acagtcgtgc tatatttagc ccatgaaaaa tcgtcaaaaa gaacaacagt cccttcacct
     2101 ttaccctcct gaataacctt aagtgttgca ttcaactctg ggaactgtga acatgaaaat
     2161 aatatattac cttctcttga atcaattgca tcatttgcag taactgaaaa tgaaagatct
     2221 gtgcttgtgg caccaacact tttcgacaca aaggtcaacc aagaaacatc agaggtataa
     2281 gtatattcta cgtttgcttt gacattaatt gtaaaagtct gttcaagctg actgattctt
     2341 ttaccatcga tgtctgaaat agtcaaaaat ggaatagcct ttgcttgtgt aacagtaaaa
     2401 aggacaggtt gctcttcgcc gtcaactata aatttaaaac tcatctgtct attaacaaat
     2461 gtcttattct cagatacaat taatttaacg atgccgtcat cctctccttc atcagggaaa
     2521 aatcttgccc atgtatcttg ttcatttgca acaattttcc aaggtctgtt tgatcttatt
     2581 acaaatggca aacctgagga atttcctgta aatgctacat catatccggt aggagtggtt
     2641 gattctgtag catctttaaa gatgaaatac ggctctccgg aatcgtcaga attctcctta
     2701 tcacatgaag tgaaaagggc gacaaccatc aggagcagta tgtgaaatct ctttttcata
     2761 agtaaagtat ttattaattt aatattatat cattgtatgt gtttccgaag cggtctgtaa
     2821 ccactacttt aatctcccgg atacctttca aaggaaaggc cgcaaaaaag aattgagaaa
     2881 gacgaggttt taagtaattc gctttttcaa actcctgtct tgttagtttt cctttttcaa
     2941 taagtttctc catatcacta atgtattttg gatcagttga tttgaattgt ttcatctcac
     3001 ccatatactt tccattccca aaccattcaa ctttccataa cggatcccaa ttccagactt
     3061 ttgccacaac agcatctgac atatttgaaa atgaacctgg tttataaagt tccatctgat
     3121 aacttctatc cttaccaatt gttttgaaat accaactaca ctcattttca ttaaattcaa
     3181 aaatctgata gccggaagga gttccatcca tacagtaaaa tgatttccaa aatgcaccgc
     3241 atgaagaagc aacattatgt tccatgaccc ctggaataat atcaagatta ttattaatat
     3301 gagtatggcc tgtaataaaa ctcaatttat aaccagataa aatattcatg atctgttgat
     3361 gtcccagcga aagcttgtaa ttatctatcc aaggtttatt cacaggtgca tgcataacta
     3421 tgaaaatttc tgcatctttc ggaacaaact tgagatatcc tttaagccac tctatctgag
     3481 actgtgatac atcttcaata tactgtttgt ttgtattata gattatatta tcaagaacaa
     3541 taacatagtt tttgccaagc acaaatccat aatatgcagg tccgaaacag ttaccaaaag
     3601 ccttaccaca attagtatct cctataagtt ccttttgata atcatgattc ccaattgccg
     3661 agtaaaccgg agtgccaagt ttggaaattc cttgcttatg gttatcaaag agtttcatat
     3721 tatcccaaac gacatcacct aaataaacag tgacaggaat tacatttttt ttaatatatc
     3781 cgtcaatcgt tgactttaaa tcagagataa ctgtctgctc aaagatatta tactgctcta
     3841 aagtttttgt ttgaggatct cctacagcta taagaacata cttattacaa tcaggaattt
     3901 tatttaattc aaaatgtaaa ttatcagatt gttggtcaat ccttttaaaa aattgagata
     3961 ttccttcttt ggcaacagcc ctgtacccag aaggaataaa aatataaaca aattcagact
     4021 ccgttgaaac atttagttta aagactccat tttcattagt ttgagtaaaa ttaaaaccat
     4081 cagtaacaac gacattctta aggcctttcc catcagaatc aactacgcct gaaatatctc
     4141 ttccaaacag agaaagtgaa aatgtgagaa acaatactat gcccaggaat ctattcatga
     4201 ccgtttcaat aataattaat tacaatccgg attgaatagc ctgtttactc caccatacag
     4261 gtgtcttcat gttgtttgga ccccccatct gctcaagagc aattgcaaca ttagcagaat
     4321 tagtggaaac agttgttgga ggataaccaa atcttgtagg gaatacattg tcattatata
     4381 cagtgccttc tccaatagta agtaccggat atcctgtcct tcgatattca tgatatgctt
     4441 ccatacctat ccaaaagagt gcaagatatt tttgattacc tataagctct tctttgtttg
     4501 aggctaaatc ccaagaacca agttttgacg aaagaaaagt attaatctct gctgaagaaa
     4561 tagatgttgg ggtctcactg taagcgccaa gtgccgacca tttttccatc gaagctctaa
     4621 ctcctgcttc gtaatagttt tttgcagcag aagcacctcc acttattaaa ttcttaagag
     4681 cagcttccgc gagaataaat tgaatttctg cataatccat aaatgttgca gttgcatcct
     4741 ttctgcaaaa caccttataa ttcaaccacg aagtacctct gtctacagta gccctttcaa
     4801 gatctgtaca acctgactta gtaccaatcc agatattgta aggattgtca gaagctactg
     4861 tatttttttt gccaataata ggaagacggg gatcaacata tatctgattg ccattttctg
     4921 taattacagt catacttata agctgtctag ttaactttct tccactttta gtaaaatcgc
     4981 tttcggtagt tgtaccaaat gtgtttatat aagggtctac accggaaaat tttacagttg
     5041 cattatcagc atttgaggta aaaataggat acttcgaagg attagtaata atctctgtca
     5101 tttttgctcc aacattcatt tctgaacgac cgctaactct gcaaagcaat cttaaataga
     5161 gagaattatt aaatttcctc cactttgtca tattcccacc atacattcca tccatactcg
     5221 gatttaggaa aacaggattc tgggcataaa tatcatttgc gttttctaaa tcttcaaaca
     5281 aaaattgata tatatccttt tgagtgttga acttgggagt taaggttcct cctatcttgc
     5341 gacctgaaaa agcttcttca tatggtacac ttccaaacat atcggtcatg tttgaaaaaa
     5401 agagcacttt aaaggttaaa gcaattgctt caagagattt gtctccttgc tttacggcaa
     5461 gttcatgcat atgatcagta ttattgccgt aatttgcata gaaattccag aatcctcccc
     5521 aatctcttga ttcttcaaga aaatactgat gctctctacg agtcacacct cctgtatgag
     5581 cagtaaactg aactaattca ttattgtaat accaggtatc gttaagccaa ttgtttgctg
     5641 aattatataa taatggttca aacattccgg atgcttgaat catgcctaca attgctttat
     5701 tagggtttgt atttgtctct tcaaagtctt ttgtacaaga actcatgact ccaagaagag
     5761 ctattccaga tatgataaat attcttttca tcttatttcc tcttatgctt tagaattgca
     5821 gtcttaaatt aacaccatat gaacgtgtca ttggaaacgc accggtttca attccgttaa
     5881 atacatttgt tccttgcaac atacctgctt caggatcaaa ttgtggccaa ttatcccaag
     5941 aaaatatatt tgttgcaaat attgctattg aagctccttg aagaaaacca acctttttac
     6001 aaagagtttc aggcaaagaa tattcaagtc ttgcctcttt aaatttaagg aatgatgtat
     6061 cgaaagtatt agcttctcca ttgtaacgat ctagcgtcaa tgcctgataa tatgtataaa
     6121 tactgcttgt gattgtgtta ttttgtgcac atacatagga gccgtcttca tttgtagata
     6181 tgacatttac tccttctgcc actttgccgt catatcttcc atccagagag tttttcaatt
     6241 tcccttgata ggagagaata ccattagtta cagaaaacct gtggcctccc cactgatatg
     6301 taaatgccat atttaaagtt attcttttat atctaattga agtatttaat ccacctttcc
     6361 agtcaggatt gacatttcca agacaaacat ctgcagaact agtcaaagat ggaagtcctg
     6421 tagatgcatc aattaatacc tgacctgaac aatcgatgtt attaccattt gcatcaacat
     6481 agtaggatcc ttctggagct cttttgagtc c
//
LOCUS       DAOTQL010000098         6463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_9879_length_6463_cov_4.310237, whole genome shotgun sequence.
ACCESSION   DAOTQL010000098 DAOTQL010000000
VERSION     DAOTQL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9879_length_6463_cov_4.310237"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="P5293_06315"
     CDS             complement(<1..547)
                     /locus_tag="P5293_06315"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRR49550.1"
                     /translation="MVINEDFTKRYEKVPVEIFDDSKDASLIVVNKVASIIKERNKLG
                     KHTVIGLSASSSLIQVYEELAKMVANKELSFKNTHIFSVHEYYPLGKNEIQSHYRFIK
                     EYLLDYIDIPIENIHCIDSEIPKEKVHDYCIEYEKLIESLGGIDILLIDNMGFNEPGS
                     YYNSRTRLITLNYNTRVAAASE"
     gene            complement(556..1386)
                     /gene="kdsA"
                     /locus_tag="P5293_06320"
     CDS             complement(556..1386)
                     /gene="kdsA"
                     /locus_tag="P5293_06320"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRR49551.1"
                     /translation="MIYDYNIENLKKYSGDNFFLIAGPCVIESEELVFHVAEKIIDIT
                     NRLSIPYIFKASYRKANRSKADSFKGIGDEKGLSILQKLKEEFKVPVLTDIHTPAEAS
                     LAASYKVDVLQIPAFLCRQTDLLEAAASTGKVVNIKKGQFLAPISMKYAAEKVSNAGN
                     NKIIFTERGTQFGYSDLIVDFRSIPEMKSFGYPVVLDVTHSLQQPNQASGVTGGRPEL
                     ITTMAQAGIAAGVDGIFIETHPEPSLALSDGANMLRLDLLENLLQSLLKLRKVYNEIK
                     "
     gene            complement(1414..2664)
                     /locus_tag="P5293_06325"
     CDS             complement(1414..2664)
                     /locus_tag="P5293_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRR49552.1"
                     /translation="MRPNNLDDFVGQDHLVGKGSVLRNMIDSGNISSFILWGPPGVGK
                     TTLAKIIAKQLNRPFYILSAVSSGVKDVRDIIDKSRKNSLFNNSRPILFIDEIHRFSK
                     SQQDALLGAVEDGTIVLIGATTENPSFEVITPLLSRCQVYVLKPLDTKSLDVILERAL
                     KEDQILKNIDISVDEKEALYRYSGGDARKLLNILDIIVQTHQGNKKVTINNKVVIDKL
                     QENIALYDKNGEQHYDIISAFIKSIRGSDPNGAVYWLARMLAGGEDILFIARRMVILA
                     SEDIGLANPNALLLANACFDSVNKIGMPESRIILSETAIYLATSPKSNSAYLAIDAAM
                     EEVQKNGGRSVPLHLRNAPTSLMKELGYSDNYKYAHDFPDNFVEQEFLPENIESIKFY
                     EPGNNKHEIEIRKRLSVQWKKYNY"
     gene            2820..4223
                     /gene="rlmD"
                     /locus_tag="P5293_06330"
     CDS             2820..4223
                     /gene="rlmD"
                     /locus_tag="P5293_06330"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRR49553.1"
                     /translation="MSKREKEIIESVEIEDIAAEGKAIAHVDGKVVFVPFVVPGDIVD
                     ILIVSKRKNYMEGVVQNLVKPSDLRVQPFCSHYGICGGCKWQLLPYSKQLEFKQQQVY
                     DQLSRIGKVQLPAISSIIGSEKELYYRNKLEFTFSNKRWIETNKESEALSEDERLGLG
                     FHITDMFDKVLDIKQCYLQREPSNSIRLFIKDYAIKNKIPFFNLRDQNGILRNLIIRT
                     ASTGDLMVIVVFFYENKDLIYKLMDELIKQFPEITSLNYVINNKKNDSISDLSVIKYH
                     GADAIHELMEGLKFKIGPKSFFQTNSEQAYKLYSVVRDYASLSGNEIVFDLYTGTGTI
                     ALFLASRASKVIGIEYIPEAIEDARVNAMENDVFNCSFYAGDIKKILTLEFVKNNGHP
                     DVIILDPPRAGIHPDIAKVIVETKPSKVVYVSCNPATQARDISLMSEFYSITAIQPVD
                     MFPHTHHVENVVKLELK"
     gene            4235..5659
                     /gene="mnmE"
                     /locus_tag="P5293_06335"
     CDS             4235..5659
                     /gene="mnmE"
                     /locus_tag="P5293_06335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRR49554.1"
                     /translation="MEGDLYVHDTICALSTSAGMAAIAVIRVSGKDAINILSTVFKPH
                     GKLDLSDTPGHTIRYGTIYRGNELIDDVLVSIFRSPSSFTGEDSVEISCHGSTFIQKE
                     ILISLISAGARIAKPGEFTQRAFFNGKLDLAQAEAVADLIASETKSSHRIAMSQMRGG
                     FSSELSSMRQSLLDLVSLMELELDFSEEDVEFADRAKLNELLDHVINHISSLIESFKL
                     GNVIKNGVPVAIVGATNTGKSTLLNSLIGEERAIVSDIHGTTRDFIEDVVNISGIAFR
                     FVDTAGIRKAKEEIEILGIERTYDKIRSAHIVMLMLDAEREDTFAIGIKALASRIKGD
                     QLVIVLLNKCELVSKKELDTLVKNIKNQALDEGLKLYAILPISAKLNIGLDKLKTILV
                     NSQEDIASNSSEILVNNIRHFEALKLALEALERVRDGMEEKLPTDLLTQDIREAIFHL
                     GEIVGEITTDEILDSIFSKFCIGK"
     gene            6056..>6463
                     /locus_tag="P5293_06340"
     CDS             6056..>6463
                     /locus_tag="P5293_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49555.1"
                     /translation="MITRDEAIAKYDISEQWFYKKVKNAGIKAMRYGTKAYYPRKDIH
                     KLFFKRKYPEIEDWATSEELAKEFCVSRKTICALARQYDIPRQRTGRTQMISRKDWLL
                     HKVELPDLEKNYMSAEQAKALYHVGASRFYKEVN"
BASE COUNT         2093 a         1141 c         1176 g         2053 t
ORIGIN      
        1 attcagaagc agcagctacc cgggtgttat agtttaaagt tataagtctt gttcgagagt
       61 tatagtacga accaggttca ttgaatccca tattgtcaat caacaagata tctattcctc
      121 caagagattc aatcagcttt tcatattcaa tgcagtagtc atgtaccttt tctttaggaa
      181 tctcgctatc aatacaatga atattttcga tagggatatc aatgtaatcg agcaaatatt
      241 ccttaataaa acggtaatga ctttgaattt catttttccc tagtggataa tactcatgaa
      301 cactgaaaat atgtgtgttt ttgaaagata gttccttatt tgccaccatc ttagccaact
      361 cttcataaac ctgtattaga gatgatgaag cacttaaccc aatgactgta tgtttgccaa
      421 gtttatttct ttcttttata attgaagcaa ctttattaac aacaattaaa gaagcatctt
      481 ttgagtcgtc gaatatttca accgggactt tttcatatct ttttgtaaaa tcctcattaa
      541 tgaccatggc tttatctatt taatttcatt atatactttt cttaacttga gcaatgattg
      601 caggagattt tctaacaagt ccagccttag catgttagct ccatcagaaa gagctaagga
      661 tggttcagga tgtgtctcga tgaaaattcc atctactccg gcagctattc ctgcctgagc
      721 cattgtcgtt ataagttcag gccgtccacc cgttactcct gaagcctgat ttggctgttg
      781 gaggctgtgt gtaacatcaa gaaccacagg atatccaaaa ctcttcattt ccgggataga
      841 acgaaaatca acaatcagat cggaatagcc aaattgagtt cccctttctg taaagataat
      901 tttattatta cctgcatttg acaccttctc tgcagcatat ttcattgaaa taggtgctag
      961 aaactgaccc tttttgatat ttacaacttt tccggttgaa gcagccgctt caagcaaatc
     1021 tgtctgacga catagaaaag ccggaatttg gagtacgtcc actttatatg aagcagcaag
     1081 cgatgcctct gcaggagtat gaatatctgt caaaaccgga actttaaatt cttccttgag
     1141 tttttggaga atggagagcc ctttttcatc acctattccc ttaaaggaat ctgctttcga
     1201 cctgttggct tttctgtaag aagctttgaa aatgtatgga attgataacc tattagtaat
     1261 atcaattatt ttttcagcaa catgaaaaac aagttcctca ctttcaatga cacacggtcc
     1321 ggcaattaga aagaaattgt cacccgagta tttttttaaa ttttctatat tataatcata
     1381 aatcatggtt gcaaaattac aaatcttctt caattagtag ttgtattttt tccattgaac
     1441 agagagcctt tttcttattt ctatttcatg tttattattt ccaggctcat aaaattttat
     1501 actttcaata ttttccggta agaattcctg ttctacaaaa ttgtcaggaa aatcatgagc
     1561 atatttgtaa ttatcagagt aacccagttc tttcatcaat gaagttggtg cgtttctaag
     1621 atgcagtgga acagatctgc caccattctt ttgcacttct tccatagctg catcaatagc
     1681 caaatatgct gaattacttt ttggagatgt tgcaagataa attgctgttt ctgacaaaat
     1741 aattcttgac tcgggcatcc caatcttgtt aacagaatca aagcatgcat tagccagtaa
     1801 aagagcatta ggattggcca gcccaatatc ttctgaagcc aaaattacca ttcttcttgc
     1861 aataaaaagt atatcctctc cacctgctag catccttgca agccaatata cagcgccatt
     1921 aggatctgag cccctaatac ttttaataaa agcagaaata atatcgtaat gttgctctcc
     1981 gtttttatca taaagtgcaa tattttcctg caatttatca ataactactt tgttatttat
     2041 ggtaaccttt ttgtttccct gatgtgtttg aactatgata tccaatatat taagtagttt
     2101 cctggcatct ccccccgagt atcgataaag agcttctttc tcatcgacag aaatatcaat
     2161 attttttaaa atctggtctt ctttaagagc tctttccaat ataacatcaa gcgattttgt
     2221 gtccagtggt tttagaacat atacttgaca tcgtgaaagt aggggagtga taacttcaaa
     2281 agaaggattt tctgttgtag cacctattaa tacaatggtc ccatcttcta cagcgcctaa
     2341 aagtgcatct tgctgacttt tgctgaaccg gtgaatttcg tctataaaaa gaataggtct
     2401 ggaattgtta aaaagactgt tttttcttga cttatcaata atgtcacgta catctttaac
     2461 acctgaagaa actgcggaga gaatataaaa tggtctgttt agttgttttg ctataatttt
     2521 agctaatgtt gttttaccaa cgccaggagg tccccaaaga ataaacgatg aaatattgcc
     2581 tgaatctatc atgttgcgca aaacacttcc tttcccaacc agatgatctt gtcccacaaa
     2641 atcatcaaga ttattcggtc tcatccgttc gggtagtggt gttaaattcg tatttaacat
     2701 aatataaatt gtgttataaa attgacatgc ttttatatat tataaatata ctattatttt
     2761 gtatatttat aagtgactaa gttattaaaa agttaaatta gtcatccgaa aatttatata
     2821 tgagcaaaag agaaaaagaa ataatcgaat cggttgaaat tgaggacata gcagctgaag
     2881 ggaaagcaat tgcacatgtc gatggtaagg ttgtgtttgt accatttgtg gtgcctggtg
     2941 atatagtcga tattctcatt gtttccaaaa gaaagaatta tatggaaggc gtagttcaaa
     3001 accttgttaa gccatcagat ttaagagttc aacctttttg ctcacattat ggtatctgtg
     3061 gtggttgcaa atggcaacta ctaccctatt ccaaacagct tgaattcaag caacaacaag
     3121 tttatgacca gctttcaagg attggcaaag ttcaattgcc agcaatatca tcaataattg
     3181 gatctgaaaa ggagttgtat taccgaaaca aacttgaatt caccttttcg aataaaagat
     3241 ggattgagac aaataaggaa tcggaagcac tttctgaaga tgaaagattg ggacttggtt
     3301 ttcatattac tgatatgttc gataaagttt tggatataaa gcaatgctat ctgcaaagag
     3361 aaccctctaa cagcataaga ctttttatta aggattatgc cattaaaaat aaaatcccat
     3421 ttttcaacct cagagatcaa aatggtattt tacgtaattt gataataaga accgcatcta
     3481 caggtgactt aatggttatt gtggtatttt tttatgaaaa taaagacctt atatacaaat
     3541 taatggatga attaattaaa caatttcctg agattacatc acttaattat gtgataaata
     3601 ataaaaaaaa tgattcaata tcagatttga gtgtaattaa atatcatggt gctgatgcaa
     3661 tacatgagtt aatggaagga cttaaattta aaattggccc aaaatctttt tttcagacta
     3721 attctgaaca agcatataaa ttatactccg tcgttcgcga ttatgcatct ttgtctggga
     3781 atgaaatagt attcgatctg tatactggta caggaaccat agctctcttc ttagcatcaa
     3841 gagcttccaa ggtcattggt atagagtaca tacctgaagc cattgaagat gcaagagtta
     3901 atgctatgga aaatgatgtc tttaactgtt ctttttacgc aggtgatata aaaaaaatat
     3961 tgactttaga gtttgtaaaa aataatggcc atcctgacgt tataatactg gatcctccaa
     4021 gagcaggtat tcatccagat atagctaaag taatagttga aacaaaaccc tctaaagtag
     4081 tatatgttag ttgtaatccg gcaacacaag caagagatat atctttaatg tcggaatttt
     4141 attcaataac agctattcaa cctgttgata tgtttcctca tacacatcat gtggaaaatg
     4201 ttgttaaact tgaacttaaa taaatattat aattatggaa ggagatttat acgttcatga
     4261 cacaatttgt gctttatcta cttctgccgg tatggctgca atagctgtaa taagagtaag
     4321 tggaaaagat gcaattaaca tcctttctac tgtatttaaa ccacatggaa agcttgattt
     4381 aagtgacact ccaggtcata caatccgcta tggaactatt tacaggggaa atgaacttat
     4441 agatgatgta ttggtgtcaa ttttccgaag cccgagctct tttacaggtg aagattctgt
     4501 tgagatctcc tgtcacggct ctactttcat acaaaaagag atattgatct cccttatctc
     4561 agcaggagcc agaattgcta aacctggaga attcactcaa agagcatttt ttaacggaaa
     4621 gcttgatctt gctcaggctg aagctgttgc tgatttgatt gcttcagaga caaaatcatc
     4681 gcatagaata gccatgagtc agatgagagg tggtttttct tcggaacttt cctcaatgag
     4741 acagtctctt cttgatttag tttcacttat ggagcttgag cttgatttta gcgaagagga
     4801 tgttgaattt gcagatagag ctaagttaaa tgagctttta gatcatgtaa tcaaccacat
     4861 atcttcactt attgaatcct ttaagttagg taatgtaatt aaaaatggtg tacctgtggc
     4921 aattgtggga gcaacaaata caggtaaaag cactctttta aattctttaa ttggtgaaga
     4981 aagagccata gtatcagata ttcatggtac aacaagggat tttatagaag atgtggtgaa
     5041 tatctctgga atcgcattcc gatttgtaga cactgccggt ataagaaaag caaaagaaga
     5101 gattgaaatt ttaggtattg aaagaaccta tgataaaatt agatctgcac atatagtaat
     5161 gctcatgctg gatgcagaaa gagaagacac ttttgcaatt ggaattaaag ccttggcttc
     5221 caggattaag ggtgatcaat tagttatagt tcttttaaat aaatgtgagc tggttagtaa
     5281 aaaagaactg gacacccttg taaaaaatat taaaaatcaa gccttggatg aaggtttgaa
     5341 actttatgct attctaccaa tttctgccaa gttaaacatt ggactagaca aactcaagac
     5401 aatacttgtt aacagtcaag aagatattgc atctaattca agtgaaatat tggtaaataa
     5461 tattcgtcat tttgaggctt taaaattggc gcttgaagca ttagaacgtg ttcgcgacgg
     5521 tatggaagaa aaacttccaa cagatcttct tacacaagat ataagagagg ccattttcca
     5581 tcttggagag attgttggag aaattactac agatgaaata ctcgattcaa ttttttcaaa
     5641 gttttgcatc gggaaataga agttagaaaa tacccataag aaaacaccca taaaattggc
     5701 aataacctat tgaatattag ccactttgca aaggtggcta tatttatgct tttttattgt
     5761 tatttatgga tattttggct atatttgtac cacgtgcggt acaagcgctg tagaatcttt
     5821 cggcagtagg cgaaaacttt acagtacttt ataaagtttt acaacttttc gccaccagat
     5881 ggcgcaatag aggtcctgag aagcctctgc atatagaaaa tagaaatatg gtcagtgagg
     5941 gcacatacta ctccatttca gaggcttcgc ggctcctggg tgtatcacgt ccgaccatat
     6001 acgccagaat caaatccggt gacattctcg cagtccaggt ctcgtcaaga tcaaaatgat
     6061 tactcgtgac gaagcaatcg caaaatacga cataagcgag cagtggttct ataagaaagt
     6121 caagaatgcc ggcattaagg caatgcgata cggcaccaag gcttactatc cgcgtaaaga
     6181 catccataaa ctattcttca agcgcaaata ccctgagatt gaagactggg caacttctga
     6241 agagttggcc aaggaattct gtgtcagccg caagaccata tgcgccctgg cccgccagta
     6301 tgatattccg cgccagcgca caggaagaac gcagatgata agccgcaagg actggctcct
     6361 gcacaaagtt gaacttccgg atctggagaa gaattatatg agcgccgagc aagccaaagc
     6421 cctgtatcac gtgggagcca gcagattcta taaagaagtc aac
//
LOCUS       DAOTQL010000099         6329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_10108_length_6329_cov_6.014026, whole genome shotgun sequence.
ACCESSION   DAOTQL010000099 DAOTQL010000000
VERSION     DAOTQL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10108_length_6329_cov_6.014026"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..276
                     /locus_tag="P5293_06345"
     CDS             130..276
                     /locus_tag="P5293_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49556.1"
                     /translation="MTKNIIRNFRGRNWILVEWRCCGNLWELILTPMFPDRKYWNLVC
                     LKCG"
     gene            361..3927
                     /locus_tag="P5293_06350"
     CDS             361..3927
                     /locus_tag="P5293_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRR49557.1"
                     /translation="MEKVKIPFWFNENCISKIVKVSFTSEGVPDILTKNHVLLYLAAE
                     GKEVKLGEIVKAEGRTPLEAFFVRYASKNSSSLETGKTSVKALLVLDKQNDKPKNYDT
                     EARNFINDYHRWGRCKYFCPGNDGKGNKERIENFDGDIDGLISILSYKFNNVPIKQDQ
                     FRFRNCIQKKHLHKANSILSEHGKVLLAFFTAYGKTYLIPKLICDNCEPGDVALVTTP
                     ILDTMIDCVDRIKTIYYGKKLYPVTDESIRDKSTDEILCEIKKKKREGYIVVIIVSVQ
                     NLRYNDLDKNEKTKLREKFSFLNKRVSLWIRDEYHREYNGVKTSEIFKNIQTKYTVDM
                     TASTYKLLTLYGSEYSNKKIMFDYFDAKASDKFPGLPKIKLSLMDFNATNISESIRSM
                     YSAEEGYDPRKTFLLENGRFVNEKALVEEFRCTYDGVYDSAGRVYPISGRKNPLAIFD
                     DTDVKDKKVVFHVVPEGSDGNTTQEIQRKLKDLCNENVKDRKFYTATDLKKESKNNNV
                     QNVINVWISEAEKENRRDGIVVFTHRQFLTGTNVHRLGMIVLSDKVGSPDEFLQMIGR
                     LFREYNGKKCAKMFIRCPGMELTVPAFVYNVLKDKNISEKQKKIYYDSLPIVSYDGEP
                     RRISYEDAFRAYREEMNKIFERSQLTTAVISSKFPDVVKMIKGMPFDDLKSKDKTATA
                     PITPKNGAKVSKKQQKEQEKKERKHIEGELNTLKSIVNEVPSIGIIHNCKSVSEIADT
                     SLFKNYFSKNNRNLFLASLRNEEFHSSLNDWYLSKKANPKNDDLFVNTDWKAKKGLVH
                     LPEDFTGKFVGKTFRFCKKCDTILVWNALNGILPVYLRDKYGKARIVCVEPHPYYIPY
                     LRKLGFETYDYNNIPKEIKDMKFDLNILNPPFQEPLKKEAKGGSRGKDLWDKFAKDAI
                     TATKDGGHVAIILPCMWRKPDAEMYPIISQYQVEYIEMHAAKKHGGTDYREVGINIFG
                     VWTAFDCIILHKVPYYKPTLVNDFSGKQHEIDLREWPWLPGGQFSCIKKILAKKNDKK
                     CEIIFSQSAYETRKEWMSKTETKCEIIYSRSAYGSDKKWMSKTETKKYKYPCFHANKK
                     GGGFNYWYSSRNDKGHFGISKVIFSDGGYIRPYNDYKGEYGMTQHAMAIPISSKKEGE
                     LICKALNSDKFKKIVWATKWSGYQTAWTMFTYFKKDFWKYFV"
     gene            3924..4352
                     /locus_tag="P5293_06355"
     CDS             3924..4352
                     /locus_tag="P5293_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRR49558.1"
                     /translation="MRSKARAKSLGEVFTPPFLVERMLRKLPKKFFKDHKYTVLDNSC
                     GNGNFLVEVAQWRMINGISHEDAISTIYGVDIDMQNVVECRNRLLMGKKDKVLQKIVE
                     HNIICADSLDPDHNGWDEVGYMWDKSRLTSRAKVKVFFDE"
     gene            4345..5361
                     /locus_tag="P5293_06360"
     CDS             4345..5361
                     /locus_tag="P5293_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49559.1"
                     /translation="MNKKFKSFIGLPGAGEIHRCYYPFKCDTYGRGCSNNCVYCYSRS
                     VLSFRNLWDHQDPARIDPKKLNKILYEALIERKKKKDYDYIRHKQPLRLGSMTDCFSD
                     AEKKYGVTYEILKILKKYEYPYLILTKNKLIAEDKYLKVLDPNLAYTQLTITTPYDDV
                     AKIYEENASSTTERLWALKRLSEAGFQTAVRINPMWPMYKDGYFSRKVESPPFRYFDW
                     FLIDMIKEHKGHTVIAGFVRLSPWNLRWIKEKTGEDISWLFDIDEKHKNLALHFSTEE
                     KRYYYETAKKQCDKLGLEFSVCYDGDDAYEEFRYLWANRDDCCNGKGKIKGFVRAWDF
                     ERMK"
     gene            5458..5772
                     /locus_tag="P5293_06365"
     CDS             5458..5772
                     /locus_tag="P5293_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49560.1"
                     /translation="MKEVVTMVFESPKSGEEIAKKMKTSRQYVSNTLKSAMGNTYNGV
                     KKLKPHYTPYEIALEMMLMFNVKDYDVEAFFKLFPPKYREKIKEDATKRFPGSARRGK
                     GK"
     gene            5769..6107
                     /locus_tag="P5293_06370"
     CDS             5769..6107
                     /locus_tag="P5293_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49561.1"
                     /translation="MRKKDIIQIILLVTIFFCLVFFAHDIARGETTTIYDENYNVKGY
                     VRDGTIYDKNWNRTGQVRDGSIYDKDYNRQYNIRDNRIYDKDGKYRGKIEGNKVYDKD
                     YKTKYRLRDR"
     gene            6108..>6329
                     /locus_tag="P5293_06375"
     CDS             6108..>6329
                     /locus_tag="P5293_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49562.1"
                     /translation="MKRKIGIVDADIINIIKRPRQFPNLACMKLSGYHKAKGDDVKLL
                     LNYNQLDAYDRIYISKVFTETIIPSTNPFF"
BASE COUNT         2129 a          958 c         1417 g         1825 t
ORIGIN      
        1 aagaatgctt agtaaactaa gcagaagcct tggggcttgc cccaaggagt gtcaccaata
       61 tgtatttcat cattgagatt gacagttacc ttcatttgag gaagccagac cataaatgct
      121 tatgcagata tgacaaagaa tattataaga aatttcaggg gaagaaactg gatactcgtt
      181 gagtggagat gttgcgggaa tttgtgggaa ttgatactta caccgatgtt ccctgacagg
      241 aaatactgga atcttgtatg tctgaaatgt ggctaagtaa ttgaaaaata aagagaaatt
      301 tcctcttgac ttttcgtaaa aaaaatagta taatatacat atattttaac cttaaaagga
      361 atggaaaaag tgaaaatacc gttttggttc aatgagaatt gtatctcaaa aatcgtaaag
      421 gtatctttta catcggaagg tgtgccggat atcctcacca aaaaccatgt tcttttgtat
      481 ctcgccgctg aaggtaaaga agtaaaactt ggtgaaattg taaaggctga aggaagaact
      541 cctcttgaag ccttttttgt gcgttatgcc agcaaaaaca gctcttctct tgagaccgga
      601 aaaacatctg taaaggctct ccttgttctt gacaaacaaa acgataagcc taagaattat
      661 gataccgaag cccgcaactt tataaatgac tatcatcgtt ggggaagatg taaatatttc
      721 tgcccaggca acgatggtaa gggaaataag gaaagaattg aaaacttcga tggtgacatt
      781 gatggtctta tttctatcct tagttataaa ttcaataatg ttcctataaa acaagatcag
      841 tttcgtttca gaaattgtat tcagaagaaa catttgcata aagcaaattc tattctttcc
      901 gagcatggaa aggttctatt agcttttttc acggcttatg gaaagaccta tctaattcct
      961 aaactcatct gtgacaattg tgagccgggg gatgttgcgc ttgtgacaac tcccattctg
     1021 gacacaatga ttgattgtgt tgatagaatt aaaactattt actatggtaa aaaactctat
     1081 ccggtgacag atgagagcat cagggataaa tctacggatg aaatcctttg tgagattaag
     1141 aagaagaaaa gagagggtta tattgtagtt attatcgttt ctgttcagaa tctccgatat
     1201 aatgatcttg ataaaaatga aaaaactaag ttgagggaga agtttagttt tcttaataag
     1261 agagtatctc tttggatcag ggatgaatat catcgtgaat ataatggtgt gaaaaccagt
     1321 gagatattca agaacatcca gacaaaatat actgttgata tgactgcctc tacttataag
     1381 ctcctgacgc tttatggttc ggaatattcc aataagaaaa taatgtttga ttactttgat
     1441 gcgaaagcaa gtgataaatt tccaggctta cctaagatta agctttctct catggacttc
     1501 aatgccacaa atatttctga aagtatcagg tcaatgtatt cagcagaaga aggatatgat
     1561 cctcgcaaga cctttttgct ggaaaatggt cgttttgtaa acgagaaggc tttagtggaa
     1621 gaatttcgtt gcacttatga tggtgtgtat gatagtgcag gaagggtcta tcctatttca
     1681 ggcaggaaaa atcctctcgc tatttttgat gatactgatg tcaaggataa gaaggtggtg
     1741 ttccatgttg tgccggaagg ttcggatggt aataccactc aggaaattca aagaaaatta
     1801 aaagaccttt gtaatgagaa tgtgaaagac cgtaagtttt atacggcaac cgatctcaag
     1861 aaagagtcta agaataataa tgttcagaat gttatcaatg tctggatttc tgaggcagaa
     1921 aaggaaaaca ggcgtgacgg tattgttgtg ttcactcacc gtcagttttt gacaggaacg
     1981 aatgttcacc gtttgggcat gattgttctt tccgataagg ttggctctcc cgatgagttt
     2041 ttacaaatga ttggtaggct tttcagagaa tataacggaa agaaatgtgc gaagatgttc
     2101 attcgatgcc cgggcatgga gttgacagtt ccggcttttg tttataatgt ccttaaggac
     2161 aaaaacatca gcgagaagca aaagaagata tactatgaca gtttgcctat cgtttcctat
     2221 gatggggagc cgagaagaat ttcttatgaa gatgctttcc gtgcttaccg tgaggaaatg
     2281 aacaaaatct ttgagagaag tcagttgact acagctgtta tttcttcaaa atttcctgat
     2341 gtggtgaaga tgatcaaagg tatgcctttt gatgatttga aatcgaaaga caagacggct
     2401 acagcaccta ttacacctaa gaacggtgcc aaagtttcta aaaagcaaca gaaggaacag
     2461 gagaaaaaag agaggaagca tatagaggga gaattgaata ctttgaaaag tattgtcaat
     2521 gaagttccct ctatcggtat cattcataat tgtaaatccg tgagtgagat tgctgataca
     2581 tccttgttca agaattattt ttcaaagaat aacagaaatc tctttcttgc aagcttgcgt
     2641 aatgaagaat ttcattcatc cttgaatgat tggtacttat cgaagaaggc taatccgaag
     2701 aatgatgacc tttttgtgaa tacggattgg aaggccaaga agggattggt tcatctgcct
     2761 gaagacttta cgggcaaatt tgttggcaag acatttcgtt tctgtaagaa atgtgataca
     2821 atattagtgt ggaatgcctt gaatggtatt ctaccagttt atttaagaga taaatatggt
     2881 aaagcaagaa tagtgtgtgt tgagcctcat ccttattaca ttccttattt gaggaagttg
     2941 ggatttgaga cttatgatta taacaatata ccaaaggaaa taaaagatat gaaatttgac
     3001 cttaatatct tgaatccacc ttttcaagag ccattgaaga aagaagcaaa aggtggttca
     3061 agaggaaaag atttgtggga taagtttgct aaagatgcca tcacggccac gaaagatggc
     3121 ggccatgttg ccattattct tccttgtatg tggaggaaac cggatgctga gatgtatccc
     3181 attatttcac aatatcaggt tgagtatatt gagatgcacg ctgcaaaaaa gcatggaggc
     3241 acagattata gagaagttgg tatcaatata tttggtgtat ggacagcctt tgattgtatc
     3301 attctgcata aggttcctta ttataaacct acattggtaa atgatttttc cggcaagcaa
     3361 catgagattg acttgagaga atggccgtgg cttccgggcg gccagttttc ttgcatcaag
     3421 aaaattctgg caaagaaaaa tgataagaag tgtgagatta tattcagcca gtctgcatat
     3481 gagacaagaa aagaatggat gagtaaaacg gaaacaaagt gtgagattat ttatagcagg
     3541 tctgcatatg gctctgataa aaaatggatg agtaaaacgg aaactaaaaa atataagtat
     3601 ccatgctttc atgccaataa aaaagggggt ggtttcaatt attggtattc gtccagaaac
     3661 gacaaagggc atttcggtat ttccaaagta atttttagtg atggtggata tattcgtcct
     3721 tataatgact ataagggcga atacggaatg acccagcatg ctatggctat ccctatttct
     3781 tcaaaaaaag agggagaatt gatttgcaaa gctctcaata gtgacaagtt caagaagatt
     3841 gtatgggcta ctaagtggag tggttatcaa acagcgtgga caatgttcac ctattttaaa
     3901 aaagatttct ggaagtattt tgtatgagaa gtaaagcaag agcaaaatca ttgggggaag
     3961 ttttcacgcc gcctttcctt gtggagagga tgctaaggaa acttcccaag aagtttttca
     4021 aggatcacaa atatactgtg cttgacaact cttgtggtaa cggcaatttc cttgtggaag
     4081 ttgcccaatg gagaatgata aacggtatat cccatgagga tgctatatct accatctatg
     4141 gtgttgacat tgatatgcag aatgtggtag aatgtagaaa taggctattg atgggcaaaa
     4201 aagataaggt actccaaaag attgtggagc acaatatcat ctgtgccgat agcctcgacc
     4261 ccgatcacaa tggatgggat gaagtggggt atatgtggga taaatccagg ctcacatcca
     4321 gagcaaaggt aaaagtattt tttgatgaat aaaaagttta agtcatttat tggtctgcct
     4381 ggtgcaggtg aaattcatag atgttactat ccattcaaat gtgacacata tggaagagga
     4441 tgttccaata attgtgtata ttgttattca aggtctgtcc tttcattccg taatttgtgg
     4501 gatcaccaag accccgcaag aatagacccg aagaaactga ataagatatt gtatgaagct
     4561 cttatagaaa ggaagaaaaa gaaggactat gactatatcc gtcacaaaca accattgaga
     4621 cttggctcaa tgaccgattg tttttctgat gccgaaaaga aatacggtgt cacctatgaa
     4681 atcttgaaga tattaaagaa gtatgaatat ccatatctga ttttgacaaa aaacaaactg
     4741 atagcagagg acaaatatct caaggttctt gacccgaatc tggcctatac tcaattgact
     4801 atcactacac cgtatgacga tgtagccaag atatatgagg aaaacgcaag ttcgactacg
     4861 gagagattat gggcattgaa gaggttgtca gaggcaggat ttcagacggc ggtaagaatc
     4921 aatcctatgt ggcctatgta taaagatgga tatttctccc gtaaagtcga atccccacca
     4981 ttccgatatt tcgattggtt tttgattgac atgataaagg agcacaaagg acatacggta
     5041 atagccgggt ttgttaggtt gtcgccgtgg aatctgaggt ggataaagga gaaaacgggt
     5101 gaggatatat catggttgtt tgatatagat gagaaacata aaaatctcgc tctccatttt
     5161 tctacagagg aaaagagata ctattatgaa acagctaaaa aacaatgtga caagttgggc
     5221 cttgagtttt ccgtctgcta tgatggggat gatgcctatg aggaatttcg ttatctctgg
     5281 gctaatcggg atgattgttg taacggtaaa gggaaaataa aaggatttgt cagagcatgg
     5341 gattttgaga ggatgaaatg aacataatag atattgattc tttgaccggt gtattattgg
     5401 tattaacaat ttattttttc tatactatca taagagacag aagaaagggg aaatagtatg
     5461 aaagaagttg tgacgatggt attcgaaagt cctaaatcgg gtgaagagat tgccaagaag
     5521 atgaaaactt caaggcagta tgtttccaat acattgaagt ccgctatggg aaacacctac
     5581 aatggtgtaa agaagttgaa gcctcattat accccatacg agattgccct tgaaatgatg
     5641 ttgatgttca atgtcaagga ttacgacgtg gaggccttct tcaagttgtt tccaccaaaa
     5701 tacagagaaa agatcaagga agatgcgacc aagagatttc cgggctctgc gagaagggga
     5761 aaaggaaaat gagaaagaag gatataatcc agattatatt gttggttacc atttttttct
     5821 gtcttgtatt ctttgcccac gatatagccc gtggtgaaac aacaaccata tatgacgaga
     5881 attataatgt caaggggtat gtcagggatg gaaccatcta tgacaagaac tggaacagga
     5941 cggggcaggt cagggatggt tctatttatg ataaggatta taacaggcag tataacatcc
     6001 gtgataatag aatatatgac aaagatggaa agtacagagg taaaatagaa ggtaacaagg
     6061 tatatgacaa ggattataaa acaaagtata gattgaggga tagataaatg aaaaggaaaa
     6121 ttggaatagt ggatgcggat attatcaata tcataaaaag gccgcgacaa tttcccaatc
     6181 ttgcttgtat gaaattatct ggttatcaca aggcaaaagg tgatgacgtc aaattattat
     6241 tgaactataa tcaacttgat gcctatgatc ggatctatat tagtaaggtc tttacggaaa
     6301 caattattcc atcaacaaat ccttttttt
//
LOCUS       DAOTQL010000100         6285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_10192_length_6285_cov_5.269823, whole genome shotgun sequence.
ACCESSION   DAOTQL010000100 DAOTQL010000000
VERSION     DAOTQL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10192_length_6285_cov_5.269823"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="P5293_06380"
     CDS             complement(<1..246)
                     /locus_tag="P5293_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRR49563.1"
                     /translation="MLQFAQPQFLLLILLIPILFIAYAMMRRSRKKRIERFGNSSTIN
                     ELIPKASKSRGWIKLSIYSIAFFFFVIGLARPQLGAKI"
     gene            complement(251..1237)
                     /locus_tag="P5293_06385"
     CDS             complement(251..1237)
                     /locus_tag="P5293_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRR49564.1"
                     /translation="MFFEYPELLYLELILIPLIALYIWREIKGVSPAMRVSATEPWMG
                     RGGRVKKAIRHLPFIFKVIALSMIILAIARPRSSSNVEKVDTEGIDIILTIDVSTSML
                     ARDFKPDRISAAKDIAIEFIAQRPNDRMGIVVFAGESFTQCPLTTDRATLINMMKEVE
                     TGLIEDGTAIGNGLATAVARLKDSKAKSRVIILLTDGVNNRGEISPLMAAEIAKTYGI
                     RVYTIGVGAMGMAPYPVMTPFGVEIQQVKVEIDEPLLKQIAEETGGRYFRATDNTKLL
                     EIYGEINKMEKSKMSVDSFPIYKELFMTFAWIALIAIALEILTGLLITRRIA"
     gene            complement(1240..2148)
                     /locus_tag="P5293_06390"
     CDS             complement(1240..2148)
                     /locus_tag="P5293_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49565.1"
                     /translation="MDLKDTLLIGDQTVLHFKTTVPKGTKYLFPTPDNPVVQGVELVG
                     KPQIDTLQNSGGELVLDTKVTLTSFDSGSYVLPPFPAYLSKADGSIDTVWYDGGRLEV
                     KTIQIDTTTFKPFDIKGQQTYKYTVGEFIPWFAVLLALVALCYLGYKLFKNYKEKKSL
                     FKKAEPVDPPHIIALRNLERIRSEKLWQNNQEKRYYTEIVDTIRQYLEQRYGIQTMEK
                     TSAEILEMLSKFNIESKVYRELEELFHVSDLVKFAKYKATQLENEEAIPIAVRFINST
                     FLQVMEEDKDKNKGDQEDKEDKERRD"
     gene            complement(2154..3032)
                     /locus_tag="P5293_06395"
     CDS             complement(2154..3032)
                     /locus_tag="P5293_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRR49566.1"
                     /translation="MTSELLKKVRKIEIKTKGLSRQIFAGEYHSAFKGLGMAFSEVRE
                     YQYGDDVRNMDWNVTARLNAPYIKVFEEERELTVVLLIDVSGSRLFGTTDKFKKDLIT
                     EIAAVLSFSASANNDKVGALFFSSKVEKFIPPKKGRSHLLRIIRELLEFEPEDNGTDI
                     GEALRFLTNAIKRRCTAFLLSDLIDVDKDLKPRYEEALKIAVNRHDISVVSVYDPRER
                     ELPDVGLVKVRDAERGKEEWVDTSSKSVRQYFAEWNEKVMEVTQQCLRKYKVDSVSIA
                     TNEDYVKGLISLFNRR"
     gene            complement(3034..4026)
                     /locus_tag="P5293_06400"
     CDS             complement(3034..4026)
                     /locus_tag="P5293_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR49567.1"
                     /translation="METVNIKELNEKIQRESAFTDMITLEMNKVIVGQKHLVENLLIG
                     LLADGHILLEGVPGLAKTLAINTLASTMDAKFNRIQFTPDLLPADLIGTQIYSQKSEQ
                     FQIKKGPIFANFVLADEINRSPAKVQSALLEAMQERQVTIGDQTFPLPQPFLVMATQN
                     PIEQEGTYPLPEAQVDRFMLKVIVSYPKKEEEKLIIRMNNSGTFPTANTILKPEDIIR
                     ARQIVREVYMDEKIEKYIVDIVYATRTPEDYDLAGLKDLIAFGGSPRASISMAIAAKA
                     YAFIKRRGYVIPEDVRAVCYEVLRHRIGLTYEAEAENVTTENIITEIMNKVEVP"
     gene            4085..4915
                     /gene="truA"
                     /locus_tag="P5293_06405"
     CDS             4085..4915
                     /gene="truA"
                     /locus_tag="P5293_06405"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRR49568.1"
                     /translation="MRFFISISYKGTRFCGWQTQPEQLSVQDEIERALTLLLGEKCEV
                     TGAGRTDAGVHAINYVAHFDTDNPLPLQDSERVIYKINAILPSDIVVHDICNVSRTAH
                     ARYDAIGRSYCYFVHTRKDPFLPDFSYYFPYNVEIAKMNEAAKYLIGEKDFTTMAKLH
                     SDVKTNICKVTDAFWEAGSPIGVTPLLPTAFQGVLSVSHGQKYEDSGAFCFTISANRF
                     LRNMVRAIVGSLLEVGRGKNKPEWIRDILLKKNRSLAGNSVPAHPLFLTKIEYPYRTF
                     "
     gene            4932..6059
                     /locus_tag="P5293_06410"
     CDS             4932..6059
                     /locus_tag="P5293_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRR49569.1"
                     /translation="MLTNIQKKDIRHLYMTPIAQQTAFWSAVKRNLGASTIAVNFKSK
                     KASIYNSVHENSDIYSDVSVIIQHLDANHSIAYIPYGPELEPDEDYQGLFLEELSESL
                     REFLPSDCIAIRYDLCWESYWAKDPDNYDFDGRWLGEPAVNYQEMRFNINTVKWNFKK
                     ASFNILPSSTVYLDLKSDTDTILQRMKPKTRYNIGLAQRRGVTVRQLDIKSLDKWYNL
                     YTETAHRNKIVINDIKHFETLLTVNVENSASPAKVFLLLAEIDNIPLAAMFLVITGHR
                     GSYLYGASSSCHRNLMGSYALQWEAIKLSKKMGCTEYDMFGVSPNADAAHPLYGLYKF
                     KIGFGGSLYHGMGCWDYPLKSNEYAHFISSELTSQGFHLHK"
BASE COUNT         1849 a         1355 c         1060 g         2021 t
ORIGIN      
        1 tattttggct cctaattgtg gccttgcaag accgattaca aaaaagaaaa atgctataga
       61 atatatagaa agttttatcc atcctctact tttggaagct ttaggaataa gctcatttat
      121 tgtagatgag ttgccaaaac gctctattct ttttttccga ctccttctca tcatagcata
      181 agctatgaaa agaattggaa tcagtaaaat cagcagtaaa aattgtggtt gtgcaaactg
      241 taacatattc ctaagctatt cttctggtta ttaacaatcc tgtcaatatc tccaatgcaa
      301 ttgcaataag agctatccat gcaaatgtca taaaaagttc cttatatatt gggaatgaat
      361 caactgacat cttgcttttt tccattttat taatctctcc ataaatctca agcagttttg
      421 tattgtcagt agcccgaaaa tatctgccac ctgtctcctc tgcaatctgt ttaaggagag
      481 gttcatctat ttcaacttta acctgctgga tttcaacccc aaacggagtc ataacaggat
      541 atggtgccat tcccattgct cctacgccga ttgtgtaaac acgtattcca taagtcttgg
      601 caatttcggc agccataaga ggggaaatct ctcctctgtt gtttactcca tctgtaagta
      661 aaatgattac cctactcttt gccttggaat ctttgagtct tgccacagct gttgccagtc
      721 cgtttccaat cgccgtaccg tcctctataa gacccgtctc cacctccttc atcatattga
      781 tcaatgtggc tctgtcagta gttagagggc attgagtaaa actctcgcca gcaaagacta
      841 cgatgcccat tctgtcatta ggcctctgtg caatgaactc aattgcaata tcttttgccg
      901 cactaattct gtcaggttta aaatctctag ccaacatact ggtagaaaca tcgatagtta
      961 agataatatc tataccctca gtatcaactt tctccacatt gctggaagaa cgaggccgag
     1021 caattgccag aataatcata gagagagcga tgactttaaa aataaatgga agatgtctta
     1081 ttgccttttt aactcttcct cctcttccca tccatggttc agtggcagat actctcattg
     1141 caggagaaac ccccttgatc tctctccata tatacagagc aatcaacggt attaatatta
     1201 actccaaata gagcaattca ggatattcaa aaaacataat taatctctcc tttctttatc
     1261 ctctttatcc tcttgatcac ctttattttt atccttatcc tcttccatca cctgaaggaa
     1321 tgtggaattt ataaatctta cggcaatagg tatagcttcc tcattttcaa gctgggttgc
     1381 cttatacttt gcaaatttca caagatctga cacatgaaat aactcctcca gttctctgta
     1441 gacctttgat tctatattaa attttgaaag catttcaagt atctcggccg atgtcttttc
     1501 cattgtttga ataccgtacc gctgctcgag atattgtctg attgtgtcta caatctctgt
     1561 ataatatctc ttctcctgat tattttgcca caatttttca gaacgtattc tctcgagatt
     1621 tctcaaagct attatatgag gtgggtcaac cggctctgcc tttttaaaaa gcgacttttt
     1681 ctctttataa ttcttaaaaa gcttatagcc caaataacag agagctacta aagctaataa
     1741 aacagcaaac cagggaataa actctcccac tgtatattta tatgtctgtt gtcccttaat
     1801 gtcaaaaggt ttaaatgtgg ttgtatctat ttgaatagtc ttaacttcca gtcttcctcc
     1861 atcataccat acagtatcta tcgagccatc cgccttactt aaatacgcag gaaaaggtgg
     1921 aagaacatat gaaccgctat caaatgaagt taatgtaact ttagtatcaa gcaccagttc
     1981 tcccccgctg ttttgcagtg tatcaatttg aggttttcct accagttcaa ctccttgcac
     2041 caccggattg tcaggcgtag ggaagagata ttttgttccc tttggcacag tcgtcttaaa
     2101 atgcagaact gtctgatcac caatcaaaag tgtatctttc aaatccatac ggcttatctt
     2161 ctgttaaaaa gtgaaatcaa tcccttaaca taatcctcat ttgttgctat gctcacactg
     2221 tcaaccttgt acttcctcaa gcattgttgt gtaacttcca ttaccttttc attccattcg
     2281 gcaaaatatt gacgtacact tttgcttgat gtatcaaccc attcctcctt acccctctca
     2341 gcatctctaa cctttaccaa accaacatcc ggcaactccc tctctcttgg atcataaaca
     2401 gaaacaactg agatatcgtg tctattgaca gctatcttca aggcttcttc ataacgtggc
     2461 tttaaatctt tatctacatc aatcagatct gaaagcaaaa atgcagtgca tcttctttta
     2521 atggcatttg taagaaatct cagcgcttca ccaatatcgg taccattatc ttccggttca
     2581 aattcaagta actcacgaat tattctcagc aagtgacttc ttcctttttt tggaggtatg
     2641 aacttttcaa ccttactact gaaaaataat gcaccgacct tgtcattgtt tgcagaagca
     2701 gaaaatgaga gaactgcagc aatttctgta atgagatctt ttttgaattt gtcagttgta
     2761 ccaaaaagtc ttgaaccact gacatcgatc aaaagtacaa ctgtcaattc cctctcttcc
     2821 tcaaaaacct taatataagg agcattaagc cttgcggtaa cgttccagtc catgttcctc
     2881 acgtcatcac catactgata ttcacgcact tcactaaagg ccatgcctag tcctttgaaa
     2941 gcagaatgat attcgccggc aaaaatttgc cttgagagtc cttttgtctt aatctcaatt
     3001 tttctgactt ttttcaacaa ctcgctggtc atattaagga acctccactt tattcataat
     3061 ctctgttata atattctcag tagttacgtt ttcggcttcg gcttcatatg tgagacctat
     3121 tctgtgcctc agcacctcat agcaaacagc tcgaacatct tctggtatca cataacctct
     3181 tctctttata aaagcataag cttttgctgc aatagccata cttatactgg cacgaggcga
     3241 tcctccgaaa gcaatcagat ccttcaaacc ggccaaatca taatcttcag gtgttcttgt
     3301 agcatatact atatccacaa tatatttttc aatcttttcg tccatataaa cctctcgaac
     3361 tatttgtctg gctctgataa tatcttcagg tttgagaata gtgttggctg tcgggaatgt
     3421 accactgttg ttcatgcgga ttataagttt ctcctcctct ttcttaggat aagaaactat
     3481 tactttcaac ataaaacggt ctacctgggc ctcgggaaga ggatatgttc cctcttgttc
     3541 aataggattt tgtgtagcca taacaagaaa gggttgaggt aacggaaatg tctggtctcc
     3601 aattgtcact tgtctctcct gcattgcctc aagcagagct gactgaactt ttgcgggaga
     3661 acggtttatc tcgtctgcca gtacgaaatt tgcaaatatg ggacctttct ttatctgaaa
     3721 ctgttcgctt ttctgactgt aaatctgtgt tccaatcaaa tcagcaggca gaagatctgg
     3781 ggtgaactgt attctgttga atttggcatc cattgtagat gcaagagtat ttatagccaa
     3841 tgtcttcgcc agaccgggaa ctccttcaag cagtatgtgt ccgtcggcaa gaagaccaat
     3901 cagaagattt tccaccaaat gcttttgtcc tacaatcacc ttattcatct caagagtaat
     3961 catatctgta aaagcactct cccgctgaat cttttcgttt aattctttga tatttacggt
     4021 ttccatattt attgtttttg tatttttgta ctataattca caaatttaat ccaaatttta
     4081 accaatgcgt ttttttatat caatttccta caagggcaca cggttctgtg gctggcagac
     4141 acaaccggaa caactctcag ttcaggacga gattgaacgg gctttaactc tccttttggg
     4201 agagaaatgt gaagtaaccg gcgcaggaag aacagatgcg ggagtccatg caattaacta
     4261 tgttgcacat ttcgacaccg acaatcctct gcctcttcag gattccgaga gagtaattta
     4321 caaaataaat gccattctcc catctgacat tgtcgttcat gatatatgca atgtcagtcg
     4381 cactgctcac gcacgttatg atgctatagg cagaagttac tgctactttg ttcacacacg
     4441 caaagatccc tttctgcctg acttttcgta ttatttcccc tataatgtgg agatagcaaa
     4501 gatgaatgag gctgcaaaat accttattgg agaaaaggat tttacaacta tggcaaaact
     4561 gcattctgat gtgaaaacta atatttgtaa agtaacagat gctttctggg aagcggggtc
     4621 accaataggt gtcacacctc ttttaccgac tgcatttcag ggagttttgt cagtaagtca
     4681 cggtcaaaaa tacgaggatt caggagcctt ttgctttact atttcggcca accgctttct
     4741 gaggaacatg gtaagagcta ttgtgggatc acttctcgaa gttggacgtg gcaagaacaa
     4801 acccgaatgg attagagaca ttctacttaa aaaaaacaga agtcttgcgg ggaattctgt
     4861 tcctgcacat cccctttttt taaccaaaat tgaatatccc tatagaacat tttaattgct
     4921 atctttgtcg tatgcttacc aatattcaga agaaagacat acggcatctt tacatgacac
     4981 caattgcaca acagactgca ttttggtctg cagttaaaag aaatctggga gcttcaacaa
     5041 ttgctgtcaa tttcaaatca aaaaaagcat caatttataa ttctgtacat gaaaattcag
     5101 acatatattc tgatgtatct gtcataattc aacatttgga tgcaaatcat tccatagcat
     5161 atattcccta tggtcctgag ctggagcctg atgaagatta tcaaggactt tttctcgaag
     5221 agctgtctga atcgctaaga gaatttctcc catctgactg tatagccata cgatatgatt
     5281 tatgctggga atcttattgg gcaaaagatc ctgataatta cgactttgat ggaagatggc
     5341 ttggagaacc ggctgtaaat taccaggaaa tgcgatttaa cattaatact gttaaatgga
     5401 atttcaaaaa agcatctttt aacatacttc catccagtac tgtttacctt gatctaaagt
     5461 ctgatactga cactattctt caaagaatga aacctaaaac cagatataat attggtctcg
     5521 ctcaaagaag aggtgtcaca gtgaggcaac ttgatatcaa aagtctcgac aaatggtata
     5581 atctttatac ggaaactgca catagaaaca aaatagtaat taacgatatc aaacactttg
     5641 aaacattgct tactgttaat gtggaaaaca gtgcttcacc tgccaaagta tttttgctcc
     5701 tggccgaaat agataatatt cctctggctg ccatgtttct ggtaatcacc ggacataggg
     5761 gatcttatct gtatggagct tcctcatctt gtcacagaaa tttaatgggc agttatgcac
     5821 ttcaatggga agcaataaaa ttatcaaaaa aaatgggatg taccgaatat gacatgttcg
     5881 gtgtttctcc caatgctgat gccgcccatc ctctatatgg tctatataaa tttaaaatag
     5941 gcttcggagg cagtctctat catggaatgg gatgctggga ttatccctta aaatcaaacg
     6001 aatatgctca ttttatctcc tcagaattaa ccagccaggg atttcatctg cacaaataaa
     6061 tataagttca ataaaaatca tcattttttc atatttttac tgcaatactt aggctcttca
     6121 ttgcgtgaat gcttttaaga aaatatttac tttcactacc gtctgttgga tgactttctt
     6181 gctattacag accgaatatt gtcatgccca acagaagagt gatattgaaa ttaacaagat
     6241 taatattaaa aaatccaagg actcttcggg cagagtcgtt ttgcc
//
LOCUS       DAOTQL010000101         6120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_10509_length_6120_cov_5.311459, whole genome shotgun sequence.
ACCESSION   DAOTQL010000101 DAOTQL010000000
VERSION     DAOTQL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10509_length_6120_cov_5.311459"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2702
                     /gene="mutS"
                     /locus_tag="P5293_06415"
     CDS             120..2702
                     /gene="mutS"
                     /locus_tag="P5293_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRR49570.1"
                     /translation="MNEDKKIAETPLMKQYFSVKSKYPEAILLFRVGDFYETYGEDAI
                     VASKILGIILTRRANGSAKHVEMAGFPYHAIDTYLPKLVRAGYKVAICDQLEDPKLAK
                     KIVKRGITELVTPGVVYNEQLLTNKENNYLAAAYFEGDKAGVAFLDISTGSFKVSSGD
                     LQYIELLLNSFAPKEILLQKSYKKGFEERFGRDIYISTMDEWAFVTDSAREKLQTHFG
                     VTTLKGFGIDQYPLAVTAAGAILYYLELTQHEGIGHICSISRIDEGDFVWIDRFTFRN
                     LEVFKSASENGSSLTEVIDRTSSPMGARLLRNWLAMPIKNVSEIEHRQNIVEKFLSDQ
                     KMRDRLRQLLKEIGDQERIISRAAAGKITPREVVQLRRGLENTKPIKDILNSSGLKEL
                     TDISLEVDPCDELLDRLTRELIEDPASQLGKGDVIANGISQELDSLRKIVRHGKEILD
                     NIQAREIEKTGISSLKVSYNNVFGYYLEVRNTHKEKVPNDWIRKQTLVNAERYITQEL
                     KEYEEKIMGAEEKIINLETSLFFEIISAIRKNIATMQKNDALLSKIDLLASFAELAHS
                     NNYKRPLVNLEDKIIIQKGRHPVIETIMAPGEEYIPNDLYLDNSTQQIMILTGPNMSG
                     KSALLRQTALIVLLAQIGSFVPAESATIGMIDKIFTRVGATDNISRGESTFMVEMLET
                     ATILHNLSNRSLVLLDEIGRGTSTYDGMSIARAIIEYIHEHKEFRAKTIFATHYHELN
                     DLENMFERVKNYHISVKEVENRVVFLRKLLPGGVAHSFGIHVARMAGMPQEVVQRAER
                     ILKQLENKKEKKDNAIQLSLFQLEEDPLLTSIRDELKEIDINKISPLEAFDILRTLKQ
                     KAGL"
     gene            2721..3992
                     /locus_tag="P5293_06420"
     CDS             2721..3992
                     /locus_tag="P5293_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR49571.1"
                     /translation="MVKLYRQITIISKLVYESILFAYSSLKGDKFRTFLSLFGVSIGI
                     FSIVTVFTAIDALRNNVATGLNSFGGNTLYIQEFPWAPPEGEEYKWWEYRKRPATTIE
                     EFQYIKANSKTAEASAFVIFTSKNIRYKRNSFNSGFITAPTSDWDKISNVEIENGRYF
                     SPFEAQSSTPVAVIGNSVAEALFAGEDPINKIIKVGQSNVKVIGVTKKAGESIVNIFD
                     TDNSITVPYRFMSTLVNARNTSSMICVKPLDNVDRDEFLLEMKLLMRSVRRLSPKQSD
                     NFALNEMTFLLNQTKSIFKGINLAGWIIGGFSILIGGFGIANIMFVSVKERTNLIGIQ
                     KALGAKRYIILTQFLAEATFLALAGGIIGLLMTLAIVLIVGGNAAFPMELSAYNIFRG
                     LFISSIIGITAGFIPAYTAARLNPVDAINSK"
     gene            complement(3984..4808)
                     /gene="rsmA"
                     /locus_tag="P5293_06425"
     CDS             complement(3984..4808)
                     /gene="rsmA"
                     /locus_tag="P5293_06425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRR49572.1"
                     /translation="MIVTPKKALGQHFLRDLKIANRIVESLSPISSECLIQNVGPLDG
                     LHLPKFNVLEVGPGMGVLTQYLVNKDNINFKVVEIDSEAVEYLQNNIPELDGKIIQGD
                     FLKLNLESVFEGNFFVIGNFPYNISSQIFFKVLEYKERIPMVVGMVQKEVAERLSSPP
                     GNKSYGILSVLLQAWYNIDYLFTVGEHCFNPPPKVKSAVIRLTRNSRNTLGCDEALFK
                     KIVKSTFNQRRKTIRNSLKSAFKDSATLKESPLFAKRPEQLSVEEFVELAKLLSSL"
     gene            complement(4805..5794)
                     /locus_tag="P5293_06430"
     CDS             complement(4805..5794)
                     /locus_tag="P5293_06430"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRR49573.1"
                     /translation="MFWNLENFFDPFNDPSTSDDEFTPKGQRRWGWNRFAKKRNDIAK
                     VIISTGEAVSSDNNISEINYPVVIGLAEVENHFVLEQLVKATPLAMLDYKIIHRDSPD
                     ERGIDVALLYRRKYFKPVSINYYPVITSDTSFKTRLILYVKGVLEELDTLHVFVNHWP
                     SKFGGELISGPKRQAAAKKLRIACDSIFQSNNKANIVIMGDFNDTPDSDLFKKVTGNG
                     VDNSNLYNLAIGIRRLGEGTIKYRASWELIDMFFVSSNLLDRNEPVCCLDCAMRIFRP
                     EFLFEEDKIYLGKKPFRCYIGPRYNGGISDHLPVILTIDKMWENPSKIGSFVP"
BASE COUNT         2038 a         1150 c         1118 g         1814 t
ORIGIN      
        1 gctatcattt ccaaaagcaa tggaagagat agccaaagtg attaaaaacg acgaaattat
       61 tttcatacat tgttttttta gaggggaata atacaaagat aatatctatt tttgtaaaga
      121 tgaacgagga taaaaaaata gcagagaccc ccttaatgaa gcaatacttt tccgtgaaat
      181 caaagtatcc ggaggcaatt cttctattca gagtaggtga tttttacgaa acatatggtg
      241 aagacgccat tgttgcttcc aagattcttg gcattatcct taccagaaga gcgaacgggt
      301 ctgcaaagca cgttgagatg gcaggatttc cttaccatgc tattgatact tatctcccta
      361 aacttgttag agcaggttac aaagtggcaa tctgcgatca gcttgaagac ccaaaactag
      421 ctaagaaaat tgtcaaacgt ggaataactg agctcgtcac tcctggagta gtgtataacg
      481 aacaattgct tacaaataaa gagaacaatt accttgcagc tgcatatttc gaaggagaca
      541 aagccggagt agcattctta gatatctcca caggtagttt taaagtatct tccggtgatc
      601 ttcaatatat tgagcttctt ctaaacagtt ttgcacctaa ggagatattg ctccaaaaga
      661 gttacaaaaa aggatttgaa gaacgcttcg gcagagatat ctatatttca acaatggatg
      721 aatgggcttt tgtcactgat tctgcaagag aaaaattgca gactcatttc ggagttacca
      781 ctctcaaagg gttcggtata gatcagtatc cactcgcagt tactgctgcc ggggctatac
      841 tttattatct tgaactaaca cagcacgaag gaataggtca catctgctca atttcaagaa
      901 ttgacgaagg agatttcgtt tggattgacc gttttacatt ccgaaatcta gaagtattca
      961 aaagtgcttc tgaaaacggg tcttccttaa cagaggttat tgacaggact tcttcgccta
     1021 tgggagcaag gttgcttaga aactggcttg cgatgcccat aaaaaatgtt tctgagatag
     1081 aacacagaca aaatattgtt gaaaaatttc tctctgacca aaaaatgagg gacagattac
     1141 ggcagttgtt aaaagagatt ggagatcagg aacgaattat ttcaagagca gcagccggta
     1201 aaataacgcc gagagaagtt gtacagctta gaagaggatt agaaaacacc aagcctataa
     1261 aagatattct taattcttca ggtttaaagg aacttactga tatctctttg gaggttgatc
     1321 catgtgacga actactggac agacttacca gagaactgat tgaagatccc gccagccaac
     1381 tcggcaaagg tgatgttatt gccaatggaa taagccagga actcgattct ttgagaaaga
     1441 ttgtccgcca tggaaaagag attcttgata atattcaggc tagagagatt gaaaagacgg
     1501 gaatttcatc gcttaaggtc agttacaaca acgtatttgg ttactatctt gaagttcgca
     1561 acactcacaa agaaaaagtc ccaaacgact ggataagaaa acagactctt gtcaatgcgg
     1621 aaagatatat tacgcaggag ctcaaagagt atgaagaaaa aataatggga gcagaagaga
     1681 aaattataaa tctcgaaacc agtctttttt ttgaaattat ctctgctatt cgaaaaaata
     1741 tcgcaacaat gcaaaaaaat gatgcattat tgtcaaaaat tgatcttctt gccagctttg
     1801 ccgaacttgc gcattccaac aattataaaa gacccttagt taatcttgaa gataaaataa
     1861 taattcaaaa aggacgtcat cctgtaatag agacaattat ggcccctgga gaagagtata
     1921 taccaaatga tctctatctc gacaattcta ctcagcagat aatgattcta acggggccga
     1981 acatgtccgg aaagagtgca cttctaagac agactgcctt aatagttctt cttgcacaaa
     2041 ttggttcatt tgtacctgcc gaatcagcca cgatcggtat gattgacaaa attttcacaa
     2101 gagttggagc aacagacaat atttcgcggg gtgagtcaac gtttatggtt gaaatgcttg
     2161 aaacagcaac cattcttcat aatctttcca accgttctct tgtactgcta gacgaaatag
     2221 gcaggggtac aagcacatat gacggaatgt ccatagctcg tgccataata gaatacatcc
     2281 acgaacataa agaattccgc gcaaaaacaa tatttgctac tcattaccat gagttgaatg
     2341 atcttgaaaa tatgtttgaa agagttaaaa actatcatat ttcagtaaaa gaggttgaaa
     2401 acagggtggt ttttttaaga aaattgcttc ctggaggcgt agcccacagc ttcggcatac
     2461 atgtagccag aatggcaggt atgcctcaag aggtagtcca aagagctgag agaatcctta
     2521 aacagctgga aaacaagaaa gaaaagaaag ataatgcaat tcagctttca ttgtttcaac
     2581 tggaagaaga ccctcttctt acaagtatac gagatgaact taaagagata gacataaata
     2641 aaatttctcc tctcgaagca tttgatatat tacggacact taaacaaaag gcaggtttat
     2701 aattaaagtc aaaaagaaaa atggtaaagt tgtacagaca gataacaata atctctaagt
     2761 tggtttatga gagcattctc tttgcataca gttctcttaa gggtgacaaa tttcgcacat
     2821 ttctttccct gtttggagtc agcattggca ttttttctat tgttactgtc tttactgcca
     2881 ttgatgcact tcgaaacaac gttgccactg gattaaacag ttttggtggt aatacccttt
     2941 atattcaaga atttccatgg gctccacctg aaggagaaga atacaagtgg tgggaataca
     3001 gaaagaggcc agccacaaca attgaggaat ttcaatatat caaggcaaac agcaaaactg
     3061 ctgaagcctc tgcatttgtg attttcacat ccaaaaatat ccggtacaaa cgtaattctt
     3121 ttaatagtgg atttataaca gcccctacca gtgattggga caaaatatcc aatgttgaaa
     3181 ttgaaaatgg acgttatttc tcgccatttg aggcacagtc atccactcca gtggcagtta
     3241 taggcaattc cgttgcagag gctttatttg ccggagaaga ccccataaat aagataataa
     3301 aggttggtca aagcaatgtg aaagttatag gtgtcaccaa aaaggcaggt gaaagtatcg
     3361 tcaatatatt cgataccgac aactctataa ctgttcctta ccgattcatg agcactcttg
     3421 ttaacgctcg taatacaagt tcaatgattt gcgttaaacc ccttgataac gttgacagag
     3481 atgaattttt gcttgaaatg aaacttctca tgaggtctgt aagaagatta agtcctaaac
     3541 agtccgataa ttttgctcta aacgaaatga catttctgct taaccagaca aaaagtattt
     3601 ttaaaggaat taatctggcc ggatggatta taggaggctt ttccatcctt ataggtggat
     3661 ttggtatagc aaacataatg tttgtctctg ttaaggaaag aacaaatctg attggaattc
     3721 agaaagcact cggtgccaaa agatatatta ttcttaccca gttccttgca gaagccactt
     3781 ttcttgctct tgcaggaggg attataggtc ttctaatgac tctggctata gtacttattg
     3841 tcggaggtaa cgcagcattt ccaatggaac tgagcgccta taacatattc agaggccttt
     3901 tcatttcatc gattatcggc ataacagcag gctttattcc tgcatatact gcagcacggt
     3961 taaacccggt ggatgctatc aactcaaagt gaagataaca gttttgccaa ctctacaaat
     4021 tcttcaacag agagttgttc gggacgtttg gcaaaaagtg gcgactcttt caaagttgcc
     4081 gaatctttga atgctgattt aagcgaattc ctaatggttt ttctcctttg attaaaagtg
     4141 cttttcacaa tttttttaaa aagagcttca tcacatccaa gagtattcct cgagtttctt
     4201 gtcaatctta ttacagccga cttgaccttt ggaggaggat taaaacaatg ttcacctact
     4261 gtaaaaagat aatcaatatt ataccatgcc tgcaataaaa ctgaaagtat tccgtaactc
     4321 ttattgccgg ggggagagga taatctctcg gcaacttcct tttgtaccat acccaccacc
     4381 attggaatcc tttccttata ttccagcact ttgaaaaaaa tctgtgacga aatattataa
     4441 ggaaaattcc cgataacaaa aaaatttcct tcaaaaacac tttccaggtt caatttcaga
     4501 aaatcccctt ggataatttt tccatccagc tcaggaatat tattttgaag atactccact
     4561 gcctcactgt ctatctcaac caccttaaaa ttaatattgt ccttgttaac gaggtactga
     4621 gtaagcacgc ccattcccgg tccaacttcc aaaacattga attttggtaa atggagtcca
     4681 tcaagaggac cgacattctg aattaaacac tcggaactaa tcggtgagag gctttccaca
     4741 attctgttag caattttcaa atctctaaga aaatgctgcc ccaaagcttt ttttggtgtg
     4801 acgatcatgg aacaaagcta ccgattttgg aaggattttc ccacatttta tcaatggtga
     4861 gtataacagg aagatgatca cttatacctc cgttatatct agggcctatg tagcaacgaa
     4921 aaggcttttt cccaagataa attttatcct cttcaaataa aaactcaggt cggaatatcc
     4981 gcatagcaca atccaaacag caaacaggct cgtttctgtc taataaattt gaagagacaa
     5041 aaaacatatc aatcaactcc catgatgccc tgtattttat agtcccctct ccaagccttc
     5101 ttatccctat agccaaatta tacaaatttg aattgtctac tccgtttcca gtcacttttt
     5161 taaaaagatc cgaatcaggc gtatcgttaa aatcccccat tataacaata tttgctttgt
     5221 tattagattg aaaaattgaa tcacaggcga ttcgaagttt tttggctgct gcttggcgtt
     5281 ttggccctga aatcaattct cctccaaatt tagacggcca gtgattaaca aaaacatgca
     5341 gagtgtccaa ttcctccaat acgcctttta cataaagaat taatcttgtt ttaaaagaag
     5401 tatccgaggt tataacagga taataattga tactgacagg cttaaagtat tttcttctat
     5461 aaagcaaagc tacgtcaata ccacgctcat caggagagtc tctgtgaatt attttataat
     5521 caagcatggc aagtggtgtt gcttttacaa gttgttccag cacgaaatga ttttcaactt
     5581 ctgccaaacc tattacaaca gggtaattaa tctcacttat attattatca ctgctcacag
     5641 cttcacctgt tgagataatt actttcgcaa tgtcatttct ttttttggca aatctattcc
     5701 aaccccatct tctttgcccc tttggagtaa attcatcatc agaagtagaa ggatcattaa
     5761 agggatcaaa aaaattttcc aaattccaaa acataatttt atattttgaa tttattattt
     5821 gagcactaca acggttgtta aaactaaaga gcaaactcga aagcagtacg atttttatta
     5881 taattaatat ccttaaaatc aacatctctg tactatagtc attttattca gactgatata
     5941 aaaaataatt aatattataa aaatacaaat cgtctgtaaa tactaagagc attttatgtt
     6001 aattatctga ttttgtggtg tttttgttta ttattaagtt ttttggcaaa aattaattaa
     6061 tttcggactt tataaaatta tagaaaatgt cactgcaatg tggtatagtc ggccttccta
//
LOCUS       DAOTQL010000102         6058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_10633_length_6058_cov_4.608362, whole genome shotgun sequence.
ACCESSION   DAOTQL010000102 DAOTQL010000000
VERSION     DAOTQL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10633_length_6058_cov_4.608362"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="P5293_06435"
     CDS             complement(<1..1205)
                     /locus_tag="P5293_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRR49574.1"
                     /translation="MKFSVKIRQQDLSDCGVASLASVAAFYGLRFPVAKLRNYSGTNS
                     EGSTIAGLIDAAKKIGLTAKGYKGKLSSLYKIPKPAILHLKKKSGLLHFAVLYRIGKK
                     FAHIMDPSKGRIQRVNIADLCEEWTGHIIVALPDDNFKKGRVGVNPLQRLWKIFKTKK
                     RDYLKALTVSILFILISLSTSFFIKYLIDDVIPSGNQHQITLIGVAMICFAILSFLLY
                     WHRSKILLNLSVGTNHELISSFVTKLFELPQQYFDSRKTGEITSRIEDAFKVGNMLSE
                     AVLTFSINILTFLLSIIVLLTVQWKLSLFLLAFLPLYILLYIIYDIINRSIRKTIMEN
                     GAKFESSLIENIRSSLTVKCFGIEKEIVADIRDRLITLNSSIVKAGKWGISFAGSTDF
                     NSKLLSITT"
     gene            complement(1501..2022)
                     /locus_tag="P5293_06440"
     CDS             complement(1501..2022)
                     /locus_tag="P5293_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRR49575.1"
                     /translation="MGGTFTVLDYCILALFIPPIISGIIKGFVSQAVSLIAIIVGIWA
                     AYSFSDIFSPYLKNCFRSDSSLINLLSFAIIFIVILIIGYFAGKLASKFIKLIMLGWL
                     DKLLGALFAIIKCAVIISVLIYIFNSLNTHLNFIPQSTLSQSKVYTFVGKIAPKIYPY
                     FEQIPGVRELMDI"
     gene            2085..3035
                     /locus_tag="P5293_06445"
                     /pseudo
     CDS             2085..3035
                     /locus_tag="P5293_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            complement(3024..3974)
                     /locus_tag="P5293_06450"
                     /pseudo
     CDS             complement(3024..3974)
                     /locus_tag="P5293_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            4037..4558
                     /locus_tag="P5293_06455"
     CDS             4037..4558
                     /locus_tag="P5293_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRR49576.1"
                     /translation="MGGTFTVLDYCILALFIPPIISGIIKGFVSQAVSLIAIIVGIWA
                     AYSFSDIFSPYLKNCFRSDSSLINLLSFAIIFIVILIIGYFAGKLASKFIKLIMLGWL
                     DKLLGALFAIIKCAVIISVLIYIFNSLNTHLNFIPQSTLSQSKVYTFVGKIAPKIYPY
                     FEQIPGVRELMDI"
     gene            4854..>6058
                     /locus_tag="P5293_06460"
     CDS             4854..>6058
                     /locus_tag="P5293_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRR49577.1"
                     /translation="MKFSVKIRQQDLSDCGVASLASVAAFYGLRFPVAKLRNYSGTNS
                     EGSTIAGLIDAAKKIGLTAKGYKGKLSSLYKIPKPAILHLKKKSGLLHFAVLYRIGKK
                     FAHIMDPSKGRIQRVNIADLCEEWTGHIIVALPDDNFKKGRVGVNPLQRLWKIFKTKK
                     RDYLKALTVSILFILISLSTSFFIKYLIDDVIPSGNQHQITLIGVAMICFAILSFLLY
                     WHRSKILLNLSVGTNHELISSFVTKLFELPQQYFDSRKTGEITSRIEDAFKVGNMLSE
                     AVLTFSINILTFLLSIIVLLTVQWKLSLFLLAFLPLYILLYIIYDIINRSIRKTIMEN
                     GAKFESSLIENIRSSLTVKCFGIEKEIVADIRDRLITLNSSIVKAGKWGISFAGSTDF
                     NSKLLSITT"
BASE COUNT         1974 a         1055 c         1055 g         1974 t
ORIGIN      
        1 aaggtggtaa ttgaaagcaa ctttgagtta aagtcagttg atcccgcaaa tgaaatcccc
       61 catttacctg ctttaacaat tgaactgttg agagttatca gtctatcacg aatatcggca
      121 acaatttctt tttcaatccc aaaacatttt acagtaagag aacttctgat gttttcaatt
      181 aaggagcttt cgaatttagc accattttcc attattgttt ttcttatgct acgatttatt
      241 atgtcatata tgatataaag caaaatataa agaggcagga acgccaataa aaacaaggaa
      301 agcttccatt gaacagttaa aagaacaata attgaaagaa gaaaagtcaa tatattaatt
      361 gaaaaagtca aaacagcttc tgacagcata tttcccacct taaaagcgtc ttcaatccgg
      421 gaagtaattt caccagtttt tctactatcg aaatattgtt gcggcagctc aaacaatttt
      481 gtaacgaatg atgaaatcag ttcatggttt gtgccaacag agagattaag caaaattttg
      541 gatctatgcc agtaaagaag gaatgataag atagcaaaac agatcattgc tactccgatc
      601 aaagtgattt gatgctgatt tcctgatggt attacatcgt caatcaaata ttttataaaa
      661 aacgaagtac ttaaagaaat taatatgaaa agtattgaaa ctgtcaaagc tttaaggtaa
      721 tctctctttt ttgtcttaaa tattttccac aatctctgaa gagggttgac tccaactctg
      781 ccctttttaa aattatcgtc aggtaaggca actattatgt gaccagtcca ttcctcgcac
      841 aaatctgcta tgtttactct ttgtattcgc ccttttgaag gatccatgat atgagcaaac
      901 tttttgccaa tccggtaaag gacagcaaaa tgaagcaggc cactcttttt cttcaaatgg
      961 agaatagccg gtttgggtat tttatacaga gatgacagct ttcctttata tccttttgca
     1021 gttaaaccga ttttttttgc agcatctata agtccggcaa ttgtacttcc ctctgaattg
     1081 gtaccactat agtttctcaa ttttgcaacg gggaatcgta acccgtagaa agctgccaca
     1141 gaagcaagtg aggcaacgcc acaatcactt aaatcttgtt gtctgatttt tactgaaaac
     1201 ttcattttct cctcctcttc ttcccataca caaattttga agttaaagaa agggggtcaa
     1261 acattttatc aacattctat aaaacatcta taactatgaa gaaattcaac cccgctggcc
     1321 tcaccctcaa aagaccattt ctctcccaca atttgacccc cttgtggaag caaatgtaca
     1381 tttgaggttt gtcatcaggg tgccaaaata actaaactga tataagttgc ttaaaagagc
     1441 attttgttgc ttattttaaa tttcagcaat tatttttaaa acagatattt ctaatttgct
     1501 ttaaatatcc attaactctc tgacaccagg gatttgttca aaataggggt agattttagg
     1561 ggctatcttt ccaacaaaag tatagacctt agattgagat aaagttgatt gagggataaa
     1621 atttaaatga gtgtttagtg agttaaagat atatattaat acactaatta ttacagcaca
     1681 ttttataata gcgaataatg cacctaacaa tttgtccaac cagccaagca ttataagttt
     1741 aatgaatttt gaggctaatt tcccggcaaa atatcctata atcagaataa caataaaaat
     1801 aatagcaaat gaaagaagat taattagcga agagtcactt ctaaaacaat ttttcaaata
     1861 tggagaaaaa atgtctgaaa agctgtatgc tgcccagata ccgacgataa tggctattag
     1921 tgagactgcc tgactgacaa aacctttaat gatcccagat ataattggtg gaatgaagag
     1981 tgcaagaata caatagtcga gaactgtaaa tgtgcctccc ataatcaatt tttttcaaaa
     2041 gtagtgaata atcgtatctt tgcaaattca aaattataag tagcatgagg tttgctgaat
     2101 acaaaagttt agatcttgta aacattaata aatccatatt gaacaagtgg aatagagaag
     2161 actcttttgc taaaactctt aaagaaagag ccgaagctga aaaatttatt ttttttgaag
     2221 gtcctccatc tgccaacggc atgcctggta ttcaccacgt aatggcaaga tctattaaag
     2281 atgtattttg cagatataaa acccaaaagg gatatcttgt cgagcgaaag gcaggttggg
     2341 atacacatgg tctgcctgtt gaacttggag ttgagaagat gcttggtata accaaagaag
     2401 atattggaaa aagtatatct gttgaagaat ataatgctat ttgccgtaaa gaggtaatga
     2461 agtatacaaa agagtgggag tctcttacag aacagatagg atattgggtt gacatgaaaa
     2521 atccatatat aacctatgat aaccgctata ttgaaacact ctggtattta ttaggtgata
     2581 tatatcgtaa gggattgctt tacaagggtt actcaattca gccatattct cctgcagcag
     2641 gtaccgggct aagtacacac gagcttaatc aaccagggtg ttatagagat gtgaaagaca
     2701 caacctgtgt tgtacagttt aagattgtca gaggtgagta ctctgagtgg atatacagag
     2761 atgacaatgg agaatcaata tataaagacg aaccggttga tgcttatttt cttgcatgga
     2821 ctacaacacc ttggacattg ccttctaaca ctgcactttg tgttggacct gctatagaat
     2881 atgtcagagt tatttctttc aatccatact cctgtagacc tgcgatttat atcttggcaa
     2941 aagagttaat ttctgctcat ttcaatcctg aagcagaaaa gcttagttta gacaattata
     3001 atccaggaga taagtctatc ccttataata ttataaggga tagacttatc tcctggatta
     3061 taattgtcta aactaagctt ttctgcttca ggattgaaat gagcagaaat taactctttt
     3121 gccaagatat aaatcgcagg tctacaggag tatggattga aagaaataac tctgacatat
     3181 tctatagcag gtccaacaca aagtgcagtg ttagaaggca atgtccaagg tgttgtagtc
     3241 catgcaagaa aataagcatc aaccggttcg tctttatata ttgattctcc attgtcatct
     3301 ctgtatatcc actcagagta ctcacctctg acaatcttaa actgtacaac acaggttgtg
     3361 tctttcacat ctctataaca ccctggttga ttaagctcgt gtgtacttag cccggtacct
     3421 gctgcaggag aatatggctg aattgagtaa cccttgtaaa gcaatccctt acgatatata
     3481 tcacctaata aataccagag tgtttcaata tagcggttat cataggttat atatggattt
     3541 ttcatgtcaa cccaatatcc tatctgttct gtaagagact cccactcttt tgtatacttc
     3601 attacctctt tacggcaaat agcattatat tcttcaacag atatactttt tccaatatct
     3661 tctttggtta taccaagcat cttctcaact ccaagttcaa caggcagacc atgtgtatcc
     3721 caacctgcct ttcgctcgac aagatatccc ttttgggttt tatatctgca aaatacatct
     3781 ttaatagatc ttgccattac gtggtgaata ccaggcatgc cgttggcaga tggaggacct
     3841 tcaaaaaaaa taaatttttc agcttcggct ctttctttaa gagttttagc aaaagagtct
     3901 tctctattcc acttgttcaa tatggattta ttaatgttta caagatctaa acttttgtat
     3961 tcagcaaacc tcatgctact tataattttg aatttgcaaa gatacgatta ttcactactt
     4021 ttgaaaaaaa ttgattatgg gaggcacatt tacagttctc gactattgta ttcttgcact
     4081 cttcattcca ccaattatat ctgggatcat taaaggtttt gtcagtcagg cagtctcact
     4141 aatagccatt atcgtcggta tctgggcagc atacagcttt tcagacattt tttctccata
     4201 tttgaaaaat tgttttagaa gtgactcttc gctaattaat cttctttcat ttgctattat
     4261 ttttattgtt attctgatta taggatattt tgccgggaaa ttagcctcaa aattcattaa
     4321 acttataatg cttggctggt tggacaaatt gttaggtgca ttattcgcta ttataaaatg
     4381 tgctgtaata attagtgtat taatatatat ctttaactca ctaaacactc atttaaattt
     4441 tatccctcaa tcaactttat ctcaatctaa ggtctatact tttgttggaa agatagcccc
     4501 taaaatctac ccctattttg aacaaatccc tggtgtcaga gagttaatgg atatttaaag
     4561 caaattagaa atatctgttt taaaaataat tgctgaaatt taaaataagc aacaaaatgc
     4621 tcttttaagc aacttatatc agtttagtta ttttggcacc ctgatgacaa acctcaaatg
     4681 tacatttgct tccacaaggg ggtcaaattg tgggagagaa atggtctttt gagggtgagg
     4741 ccagcggggt tgaatttctt catagttata gatgttttat agaatgttga taaaatgttt
     4801 gacccccttt ctttaacttc aaaatttgtg tatgggaaga agaggaggag aaaatgaagt
     4861 tttcagtaaa aatcagacaa caagatttaa gtgattgtgg cgttgcctca cttgcttctg
     4921 tggcagcttt ctacgggtta cgattccccg ttgcaaaatt gagaaactat agtggtacca
     4981 attcagaggg aagtacaatt gccggactta tagatgctgc aaaaaaaatc ggtttaactg
     5041 caaaaggata taaaggaaag ctgtcatctc tgtataaaat acccaaaccg gctattctcc
     5101 atttgaagaa aaagagtggc ctgcttcatt ttgctgtcct ttaccggatt ggcaaaaagt
     5161 ttgctcatat catggatcct tcaaaagggc gaatacaaag agtaaacata gcagatttgt
     5221 gcgaggaatg gactggtcac ataatagttg ccttacctga cgataatttt aaaaagggca
     5281 gagttggagt caaccctctt cagagattgt ggaaaatatt taagacaaaa aagagagatt
     5341 accttaaagc tttgacagtt tcaatacttt tcatattaat ttctttaagt acttcgtttt
     5401 ttataaaata tttgattgac gatgtaatac catcaggaaa tcagcatcaa atcactttga
     5461 tcggagtagc aatgatctgt tttgctatct tatcattcct tctttactgg catagatcca
     5521 aaattttgct taatctctct gttggcacaa accatgaact gatttcatca ttcgttacaa
     5581 aattgtttga gctgccgcaa caatatttcg atagtagaaa aactggtgaa attacttccc
     5641 ggattgaaga cgcttttaag gtgggaaata tgctgtcaga agctgttttg actttttcaa
     5701 ttaatatatt gacttttctt ctttcaatta ttgttctttt aactgttcaa tggaagcttt
     5761 ccttgttttt attggcgttc ctgcctcttt atattttgct ttatatcata tatgacataa
     5821 taaatcgtag cataagaaaa acaataatgg aaaatggtgc taaattcgaa agctccttaa
     5881 ttgaaaacat cagaagttct cttactgtaa aatgttttgg gattgaaaaa gaaattgttg
     5941 ccgatattcg tgatagactg ataactctca acagttcaat tgttaaagca ggtaaatggg
     6001 ggatttcatt tgcgggatca actgacttta actcaaagtt gctttcaatt accacctt
//
LOCUS       DAOTQL010000103         6008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_10736_length_6008_cov_4.629095, whole genome shotgun sequence.
ACCESSION   DAOTQL010000103 DAOTQL010000000
VERSION     DAOTQL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10736_length_6008_cov_4.629095"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2550
                     /gene="ileS"
                     /locus_tag="P5293_06465"
     CDS             <1..2550
                     /gene="ileS"
                     /locus_tag="P5293_06465"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRR49578.1"
                     /translation="LSLDNYNPGDKSIPYNIIGDTFKGDKLCGIRYEQLIPWVNPGEN
                     AFRVIAGDFVTTSDGTGIVHIAPTFGADDDKVAKANGIPPLTVVDKDGFKQAMVDRNG
                     KFYKIEDLDPSFVSQKVDVELYREFAGRFVKNAYDPTLSENDSTLDIDLAVMLKQQGK
                     VFKIEKHVHSYPHCWRTDKPVIYYPLDSWFIKTTSVQERMIELNKTINWKPEATGSGR
                     FGKWLENLQDWNLSRSRYWGTPLPVWRTEDGREERIISSVKELYEALEESVKAGFITS
                     NPLKDKGFIPGDFSKENYDKIDLHRPYVDEFILVSPKGQKMKRESDLIDVWFDSGAMP
                     YAQVGLEKLDNEEFGQTADFIAEGVDQTRGWFFTLHAISTMVSDKVAFKTVVSNGLVL
                     DKDGNKMSKRLGNAVDPFQTLNEYGADALRWYMLTNSQPWDNLKFDINGVDEVRRKFF
                     GTLFNTYSFFALYANIDDFTNSEPQVAFAERPEIDRWIISLLNTLIKDVICSYENWDV
                     TSAGRMIQDFVVDNLSNWYVRLNRKRFWGGEMNIDKLSAYQTLYSCLETVAILSAPIA
                     PFFMEQLFCDLNEVTGRHKESTVHSVLMPESDLSAIDKALEDRMQLAQRASSMILGLR
                     RKVNIKVRQPLAKILVPIIDDTIKEQFEKVKTLILNEVNVKDVEYIDDTTGIIIKKVK
                     PNFKTLGKKYGKQMKEISAEFTNFTQEQITVLESSEEYKMELPSGSIVLNQEDIEILS
                     EDMPGWLVAVDGKLTIALDITVTEELRREGVARELVNRIQNLRKENKFEVTDKVIVFI
                     QPLEEIVEALKYYKEYICNQTLAKDLILDPNIDGTEIEWDGGTLKIKAERA"
     gene            2566..3039
                     /locus_tag="P5293_06470"
     CDS             2566..3039
                     /locus_tag="P5293_06470"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRR49579.1"
                     /translation="MEKREDYLKKAIFLAADNVRENGGGPFGALIVRDGEVLGSGVNS
                     VTIDNDPTAHAEINAIRNACHKLGTFNLKGCTLYASCEPCPMCLSAAYWARVDGIIFA
                     ADKTNARDAGFDDAFIYEEIALPFSDRTIRTEQIAVKESNMPFEIWKESDNKILY"
     gene            3072..3479
                     /locus_tag="P5293_06475"
     CDS             3072..3479
                     /locus_tag="P5293_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRR49580.1"
                     /translation="MAKKGNKETEHLEKVRYSDEELQMFKELITEKLEAAKLEYQQLK
                     SAVTHSSSNDTEDTSPTFKVLEEGASALSKEEAGQLAQRQYKFIQSLEAALVRIENKT
                     YGICRETGKLIPKERLMLVPHATLSVEAKKKRD"
     gene            3483..4133
                     /locus_tag="P5293_06480"
     CDS             3483..4133
                     /locus_tag="P5293_06480"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRR49581.1"
                     /translation="MHLSKGKKITIFIVLILLIDQVVKILIKTNMKIGESIRIFDWFQ
                     ILFVENNGMAFGMELGGTLGKLALSLFRIGLAVFIVYYIRKLIAHNAPMGVLVGVALI
                     LVGAVGNIFDSLFYGLIFSESTPTQVAVLFPPGGGYAPFLFGKVVDMLYFPLIDTIMP
                     NWVPFIGGERFIFFRPIFNIADSCITIGVIYMLLFERKYFTSASPSASDGKNVSGS"
     gene            complement(4081..4566)
                     /locus_tag="P5293_06485"
     CDS             complement(4081..4566)
                     /locus_tag="P5293_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR49582.1"
                     /translation="MKTTFEKDILELEESLSRYAYSLTFNRDDAKDLVQETFFKALNN
                     QEKFSDQTNIKAWVYTIMKNTFINDYRRRTKRQALFSSDVQEYVLNNRPAEHNPESEF
                     SFRELTKVVNSLEPEFRVPFQMHDSGYKYQEIADELGLHLGTVKSRIHFSRQKLMEKL
                     K"
     gene            4694..4778
                     /locus_tag="P5293_06490"
     tRNA            4694..4778
                     /locus_tag="P5293_06490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4728..4730,aa:Ser,seq:tga)
     gene            5067..>6008
                     /locus_tag="P5293_06495"
     CDS             5067..>6008
                     /locus_tag="P5293_06495"
                     /inference="COORDINATES: protein motif:HMM:NF027696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PL29 family lyase N-terminal domain-containing
                     protein"
                     /protein_id="HRR49583.1"
                     /translation="MKRKFTFFGICAFMCALVLSLGSCQKDYSDDIDQLKADVAANKL
                     AITSLQQAITAGKLITSVTQNTTGYLIKFSDNTTIQVNNGTPGATGPTGPTGPTGPTG
                     ATGATGFTPIVGIDAEGYWTVIASSGGTATRILDANGHEILAKIDNNNVSSANGYLTV
                     NGVATNVVIPTIVFDEVNHVLVITIPDPDNNNTLTSYNVGLAADYVLGNDIISLLSPI
                     GTTKAVISFGAVPATAAAYTAAYGAPLSAGAQTWSGVTYGSLLRSSGKLPVIVNPAEA
                     SIAGYTFEVIKQDGSNYVIQPSSIVQGYSGAFAQYVDG"
BASE COUNT         1836 a         1007 c         1306 g         1859 t
ORIGIN      
        1 cttagtttag acaattataa tccaggagat aagtctatcc cttataatat tataggagat
       61 acatttaagg gcgataaatt atgtggcatt cgttacgagc aattgatccc atgggtaaat
      121 cctggtgaaa atgcttttag agtcattgct ggagattttg ttacgacatc tgatggaaca
      181 gggattgttc atatcgcacc aacgtttgga gctgatgatg acaaggttgc caaggcaaac
      241 ggtatacctc ccttaacggt tgtggacaag gatggattta agcaggctat ggttgacaga
      301 aatggaaagt tttacaaaat tgaagatctt gatccttcat ttgtttcgca aaaagttgat
      361 gtggagcttt atagggaatt tgccggcagg tttgtgaaaa atgcgtatga tccaacattg
      421 agtgaaaatg attctactct tgacatagat cttgcagtaa tgctcaaaca acagggaaaa
      481 gtatttaaga ttgagaagca tgtccactct taccctcatt gttggcgtac tgacaaacct
      541 gtaatttact atccattaga ttcctggttt ataaagacta cttcagttca ggaaagaatg
      601 atagagctta acaaaactat caactggaaa cctgaagcta ctggaagtgg acggtttggc
      661 aagtggctgg aaaatcttca ggattggaac ctttcaagaa gccgttattg gggaactcct
      721 ttgccggttt ggagaactga agatggcagg gaggagagga taatatcatc tgtcaaagag
      781 ctgtatgagg cactcgaaga gtctgtcaaa gctggtttta tcacctcaaa tccgttgaag
      841 gacaagggat ttattccagg tgatttcagt aaggagaatt atgataaaat tgatttgcat
      901 agaccttacg ttgacgagtt tattcttgtg tctccaaagg gtcaaaagat gaagagggag
      961 agtgatttga tagatgtttg gtttgattcg ggggcaatgc catatgctca agtgggactt
     1021 gaaaagcttg ataatgaaga atttggtcaa actgcagatt ttattgctga gggagtagac
     1081 cagacaagag gctggttctt tacacttcac gctatttcga caatggtcag cgataaagtg
     1141 gcattcaaaa ccgtagtttc caatggtttg gttcttgaca aagatggcaa taagatgagc
     1201 aagaggcttg gaaatgccgt tgatcctttt cagacactaa atgaatatgg agcagatgct
     1261 ttaagatggt atatgctgac aaactctcaa ccttgggata atctcaagtt tgatatcaac
     1321 ggagtagatg aggtgagaag aaagtttttt ggcacacttt tcaacactta ttcttttttt
     1381 gccctttatg ccaatataga cgattttaca aattcagaac ctcaggtagc ttttgcagaa
     1441 aggcctgaaa tagatcgctg gataatatct cttcttaata cactaattaa ggatgttata
     1501 tgttcttatg agaattggga tgtaacatct gccgggagga tgattcagga ttttgttgta
     1561 gataatctca gtaattggta tgtaagactt aatagaaaaa gattttgggg aggggaaatg
     1621 aatattgata aattatcggc ctaccagacc ctctatagct gtcttgagac tgtagcaata
     1681 ctttctgctc caatagcacc ttttttcatg gaacaacttt tctgtgacct taatgaagta
     1741 acaggcagac ataaggagag tacagtacat tcagtattaa tgcccgaatc cgatctttct
     1801 gcaattgaca aggcattgga agatagaatg cagttggccc aaagggcatc atccatgata
     1861 ttaggactca ggcgcaaggt caatatcaag gtacgccaac ctttagccaa gattctggta
     1921 cctattatag acgatactat taaggagcag tttgaaaaag ttaagactct tattttgaat
     1981 gaagttaatg tgaaggatgt tgaatacatt gatgatacaa ccggcataat tataaaaaaa
     2041 gtcaaaccaa actttaaaac acttggcaag aaatatggca aacagatgaa ggagatttca
     2101 gctgaattta ctaactttac acaagagcag attactgttt tggagtcgag tgaagaatat
     2161 aaaatggaat taccttcagg ctctattgtt cttaatcagg aagatattga aattttatct
     2221 gaagacatgc ccggatggct ggtagcagtc gacggaaaac ttacaatcgc tcttgatatt
     2281 acagtgactg aagaattgag aagagaagga gtggcacgag aacttgttaa caggattcaa
     2341 aatctcagga aggagaataa gtttgaagtt actgacaagg ttattgtttt cattcagcct
     2401 cttgaagaga tcgttgaagc attgaaatac tataaggaat atatttgtaa tcagactttg
     2461 gcaaaggatt tgatacttga tccaaatata gacggaactg aaatagaatg ggatggagga
     2521 actttaaaaa ttaaggcaga gcgggcataa cttaaaaatt ggtacatgga gaaaagggaa
     2581 gattatttga aaaaagctat ttttttagct gctgataatg tcagggaaaa cggaggtggt
     2641 cctttcggag cattgatagt aagggatggt gaagttttgg gttcgggagt caatagtgta
     2701 actatcgata acgatccaac tgctcatgct gagattaatg caatcagaaa tgcttgtcac
     2761 aaacttggca ctttcaattt aaagggatgt actctttatg caagttgtga accttgtcca
     2821 atgtgtcttt cggctgccta ttgggccaga gttgacggga taatatttgc tgcagacaag
     2881 acgaatgccc gggatgcagg ttttgatgat gcctttatat atgaagagat agctcttcct
     2941 ttttcagatc gtacaataag gacagaacag attgccgtaa aagagagtaa tatgcctttt
     3001 gaaatttgga aagagagtga taataaaatt ctttattaat tattatattt acgtttttaa
     3061 cttaaagaga tatggcaaag aaagggaaca aagagactga acatttggaa aaggtccgtt
     3121 acagtgatga ggagcttcaa atgttcaaag agcttatcac tgagaagctg gaagctgcaa
     3181 aattggagta tcagcagctc aagtcggctg taacacatag ctctagcaac gatactgagg
     3241 atacttcgcc tacattcaag gttcttgagg aaggtgcctc tgccctttca aaagaggagg
     3301 ctggacagtt agcgcagagg caatacaaat ttattcaatc tctcgaagca gctcttgttc
     3361 gtattgagaa caaaacatat ggaatatgca gagagacagg caaacttatt cccaaggaga
     3421 ggctcatgct tgttcctcac gctacactta gcgtcgaagc taaaaaaaag cgagactgat
     3481 agatgcatct ctcaaaaggg aaaaaaataa caatcttcat tgtactgatc cttcttattg
     3541 atcaggttgt caaaattctg atcaagacaa atatgaaaat tggggagagc atcagaatct
     3601 ttgactggtt ccagattctt tttgttgaga ataatggaat ggcttttgga atggagttgg
     3661 gtggtactct gggtaaactt gctcttagcc tttttagaat tggattggct gtatttattg
     3721 tctattacat caggaagctt attgctcata atgctcccat gggagtgttg gttggcgttg
     3781 ctttaattct tgtgggtgcg gtaggaaata tttttgacag tctattttat gggttgatat
     3841 tcagtgaatc aacgccaact caagtagctg ttctttttcc gccgggtggt gggtatgctc
     3901 cgtttttatt tggcaaagtc gtcgatatgc tctacttccc attgatagat acaattatgc
     3961 caaattgggt cccttttatt ggaggagaac gctttatatt tttcaggcca atttttaata
     4021 ttgcagattc atgtattaca attggagtga tatacatgct tctgttcgaa agaaaatact
     4081 ttacttcagc ttctccatca gcttctgacg ggaaaaatgt atccggctct tgaccgttcc
     4141 caaatgaagc ccaagttcat cagctatttc ttgatattta tatccactgt catgcatttg
     4201 aaatggtact ctgaactctg gttcaagaga gttaactact tttgtcaatt ctctgaagct
     4261 aaattcactt tcaggattat gttctgccgg tctgttatta agaacgtatt cctgcacatc
     4321 gctgctgaat aaagcctgcc tttttgtccg tctacggtaa tcattgatga aagtgttttt
     4381 cattattgta tatacccagg cttttatatt agtctggtca ctaaattttt cttgattatt
     4441 cagagctttg aagaaagtct cctggacaag atccttagca tcgtccctgt tgaaggtgag
     4501 gctatatgcg tatctggaaa ggctttcctc aagttccaga atatcttttt cgaatgttgt
     4561 tttcatatag tcgtttgtgt tagtaattat tttccatcag caaatattat accatgaaca
     4621 taaatgtttg agcattaaag caaaattgtt tttttggaga aagtaaaaaa aaccatatat
     4681 ttgcagcccc acaggagagg tggcagagtg gtcgattgcg gcggtcttga aaaccgttga
     4741 actgagaggt tccgggggtt cgaatccctc cctctccgca aatgctaaaa ggaaatttcg
     4801 tcaatgactg taaccgttgg cgaaattttg ttatatatcg tgttttaatt ttttgataag
     4861 tggaaaattt taaaaaaaat atgttttata tttgctatga attatgaaaa acataataat
     4921 tgtttcaaag atgtgttgca gatattaaaa tgtttgcata catttgcgat aatatatgag
     4981 taataaggaa attgaataaa ataattacct atatttgcca atcaaaattg tttaactaaa
     5041 agaattttta acttaagaat taaaaaatga aaagaaaatt cacatttttc ggtatctgtg
     5101 cgtttatgtg cgcactcgtg ctatctcttg gttcttgcca aaaggactat tcagatgaca
     5161 tcgatcaatt gaaagccgat gttgctgcaa acaagcttgc tattacttct ttgcaacaag
     5221 ccatcactgc cggtaagctt attacaagtg ttacacaaaa tactaccggc tatttaatta
     5281 agttctcaga caatacaacc attcaggtta acaatggtac cccaggagct acaggtccta
     5341 ctggtcctac aggccctact ggtcctactg gtgctactgg tgctactggt tttactccta
     5401 ttgtcggtat tgatgccgag ggttactgga cagtaattgc atcaagtggc ggaactgcta
     5461 cccgcatatt ggatgctaac ggtcatgaaa tccttgcgaa gatcgataac aataatgttt
     5521 cttcagccaa tggctatttg acagttaatg gagttgccac taatgttgtg attcctacaa
     5581 tcgtttttga tgaagtcaat catgttcttg tgattactat tcctgaccca gacaacaata
     5641 atactctaac aagctacaat gttggtctgg cagcagatta tgtacttgga aatgatatta
     5701 tcagccttct ttctccaatc ggtacaacaa aagctgttat cagtttcggt gctgtaccag
     5761 caactgcagc tgcatatact gcagcttacg gcgctccact gtctgccggt gcacagactt
     5821 ggtctggtgt tacttatgga tcacttctac gttcctcagg aaaacttcct gttatagtta
     5881 atccagcaga agcatctatt gcaggttata ctttcgaagt tatcaagcag gatggttcaa
     5941 actatgtaat ccaaccttca tctatcgtgc agggttattc tggagctttt gctcagtatg
     6001 ttgatggc
//
LOCUS       DAOTQL010000104         5990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_10775_length_5990_cov_4.793766, whole genome shotgun sequence.
ACCESSION   DAOTQL010000104 DAOTQL010000000
VERSION     DAOTQL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10775_length_5990_cov_4.793766"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="P5293_06500"
     CDS             <1..541
                     /locus_tag="P5293_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRR49584.1"
                     /translation="GIGIIFLQGGSTGGTDIIALIISKYKETSPGRVFLFCDLIIIGS
                     VIFIPGKSLSDVIYGYIEMVSFSYVVDMILTGNKQSVQIHVFSSKYKEIADRVLKDLR
                     RGVTAVNSTGWYSQKEGRVLIIVLRKNQVTDITNLVKEIDKDAFMTVTPVMSVYGKGF
                     DNFKGGKISWKTKEETISD"
     gene            508..1407
                     /locus_tag="P5293_06505"
     CDS             508..1407
                     /locus_tag="P5293_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRR49585.1"
                     /translation="MENKGRNYFRLIKEYVIITFGLLIYVLGWSVFLVPNNLVGGGAT
                     GISAIILYATGFPISYSYIIINGILVAIALKVLGKQFGFKTVFAIIVVSIFFDIIPPA
                     IPRELIEDIAISNGKLLSAIMGAVCYGVGIAITFTQGGSSGGTDIIALMINKYRNISP
                     GKIILLCDVIIIASSMFIPNDMSLGEKLAVVIYGYILISVGSYTIDLVLSGARQSVQI
                     FIFSKKYQEIAERITSIGRGVTVIDAMGWFTKEKGKVLLVIVRKTESNFVFRVIREID
                     KNAFISVGSVMGVYGQGFDQIKK"
     gene            1541..2698
                     /locus_tag="P5293_06510"
     CDS             1541..2698
                     /locus_tag="P5293_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR49586.1"
                     /translation="MNLTSNFVEVQSCIELLFVLSYFIVSLYFPFIIFVDKSLSDKSR
                     ELVKSMGYLFSFITVILVVRLMVAFHHPGNEMFHGAILTLLGLMVITSPVRRYVCSKI
                     SVNICSARVSFIKAAAATMLYLSIVLFDIKGIMIPQSINIICTVVLILLFLSESIGII
                     IKVIKGIINTLECDRIRIARLKHDLFLSIIEALISISILFFLFTGFNDIYVWSALTLL
                     LLFNGYVVFATCSYESDNDKDVARKDVVDESEEQLSDDKNRIIRGLSDKAKADELYKR
                     LIIYFEQKKPYLQQDLKIREVALYLYSNKTYLSRIINDRHNLNFNQFVNYYRIEEVKR
                     LFSENTTLSIQELCTRSGFGSMATFSIAFRYHLGNTPADWCKEQRLLKEHE"
     gene            2691..3137
                     /locus_tag="P5293_06515"
     CDS             2691..3137
                     /locus_tag="P5293_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR49587.1"
                     /translation="MNRNLRCVIKRVLYLSGLFEKDPVLKFVKLSDSLLVGGKRAEEL
                     LLTKRLYLDPCLTVTKLAKEAATNRTYLSNYINSEKGCNFRQYVNLLRAAQALKLIES
                     EKMETLSDVAIQSGFSNLRSLNRAFIAVYGKLPSQVRREYLRDKHS"
     gene            complement(3132..4964)
                     /locus_tag="P5293_06520"
     CDS             complement(3132..4964)
                     /locus_tag="P5293_06520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRR49588.1"
                     /translation="MGTYTEKNIRTLNWNEHIRRRPGMYIGKLGDGSSPDDGIYVLLK
                     EVMDNSIDEFAMGFGKIIYVTIQDKTVSVRDFGRGIPLGSLVDASSKINTGGKYDDEA
                     FKKSVGLNGVGLKAVNALSVSCLVQSFREGESHFAKFSRGELIEEGRITTSEKNGTLV
                     TFLADDELFENFSYNFEFVESMLRNYCYLNTGLTIKFNEKEFFSKNGLLDLLNESLTK
                     EPLYQPIHLKGEDIEIAMTHGNDYGESISSFVNGQYTTQGGTHLAAFREGIAKTLKEF
                     YKRDFDPSDIRQSIIAAISIKVQEPQFEGQTKTKLGSKEMSVNGVSIRNYIIDFLKDN
                     LDNFLHKNPETADTILKKILDSEKERKAISGIQKLARERAKKANLHNKKLRDCRIHYN
                     DKNERAEESTLFITEGDSASGSITKSRDVTTQAVFSLRGKPQNSYGLTKKIVYENEEF
                     NLLQAALNIEADLDNLRYNKIIIATDADVDGMHIRLLLLTFFLQFFPELIRQGHLYIL
                     QTPLFRVRNKKETHYCYSIEERDKVMKKLGANIEITRFKGLGEISPDEFREFIGDNIK
                     LERVRLGNDDNIPQLLEFYMGKNTPDRQSFIRSHLRQEVIINEL"
     gene            complement(5003..>5990)
                     /locus_tag="P5293_06525"
     CDS             complement(5003..>5990)
                     /locus_tag="P5293_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HRR49589.1"
                     /translation="SIIKSRREWLIEKRHLEIHVVAICNSRHMILNKSGIEIDSIDES
                     LKMGDPSDIYRFRELCSDMNLPDSIFVDCTADRGVSSLYQDFMQGKCSVVTCNKIGLS
                     SSQESYDALQETCCATEVKLKYETTVGAALPVISTIKSRVECGDNIRRIEAILSGTLN
                     YLFSKYDGSAKFVDIVREAKNAGYTEPDPRIDLSGTDVLRKTVILARESGFRLEPEEI
                     DVVPIFNSDLFSYDSEEFMTKIEECEDQMLSLYKSALSRGERLRYIATIENGNYKIGV
                     QSVSSDHPFYNINGRDNAIMIFTDEYSSPLTVSGAGAGARLTASGLFADLLA"
BASE COUNT         1772 a         1045 c         1137 g         2036 t
ORIGIN      
        1 aggtataggc attatatttc ttcagggtgg aagtacagga ggaactgata ttattgcact
       61 tattatatca aaatacaaag agacttctcc cgggagggtt tttctttttt gtgatttaat
      121 aattattggt tcggtaatct tcattcccgg caaaagtctg tcggatgtta tttatggtta
      181 cattgaaatg gtctctttct cttatgtggt cgatatgatt ttaacaggaa acaaacaatc
      241 tgtccagatt catgtatttt cctcgaaata taaggaaatt gcagacagag tattgaaaga
      301 tttaagaaga ggagttacgg ctgttaattc tacgggttgg tattctcaga aagaggggcg
      361 cgtattaata atagttttga gaaaaaacca ggtaacagat attacaaacc ttgttaaaga
      421 gatagacaaa gacgcattta tgactgtgac accggtcatg agcgtatatg gcaaaggatt
      481 tgataatttt aaaggcggta aaatttcatg gaaaacaaag gaagaaacta tttcagatta
      541 attaaggagt atgtgatcat aacctttggt ctcctgatat atgttttggg gtggagcgtt
      601 ttccttgttc caaacaatct tgttgggggt ggcgcaactg gcatatctgc aataattttg
      661 tatgcaacag gattcccaat tagttatagc tatataataa tcaacggtat tctcgtggca
      721 atagccctta aggttttggg taaacagttt ggctttaaga cagtctttgc cataatcgtt
      781 gtttctatat tttttgatat tatccctccg gctattcctc gggagttaat agaggatatt
      841 gctatcagca atggaaaatt gttatcggcc attatgggtg ctgtttgtta tggggttggg
      901 attgctatta cttttactca gggaggaagt tccggaggaa cagatattat agccctgatg
      961 ataaacaaat atagaaatat ctctcccggc aagataatat tgctttgtga tgtaattatt
     1021 attgccagtt caatgtttat tccaaatgac atgtcattgg gagaaaagct ggcagtggtt
     1081 atttatggat acatacttat ctctgtgggg agctatacaa tcgatctggt attatctggc
     1141 gcaagacagt ctgttcagat ttttatcttt tcaaaaaagt atcaagagat tgctgaaaga
     1201 attacttcta ttggtcgggg agtaacagtt attgatgcca tggggtggtt tacaaaggaa
     1261 aagggtaaag tactgctggt gattgtcaga aagacggaga gtaattttgt ttttagggtt
     1321 ataagagaaa ttgataaaaa tgcctttata tctgtaggta gtgttatggg cgtttatggg
     1381 caaggatttg accaaatcaa aaagtaattt tctataattt taggcaacat tttaaaaacg
     1441 tcaaaaaatt taatcttaga taaattatta tataatagct tgttaagtta ggataatttt
     1501 aatttattat ttatttttga caaaatctaa cttaaaagct atgaatttaa cgtcaaactt
     1561 tgtggaagtt caatcctgta ttgaacttct gtttgttctc tcttatttca tcgtttcact
     1621 ctatttccct ttcatcattt ttgtcgacaa gtctctttcc gataaaagca gagagttagt
     1681 aaaatcgatg ggatatctat tctcatttat tactgttatt ttagttgtaa ggctaatggt
     1741 agcatttcac catcctggaa acgaaatgtt tcatggtgcg attttgacac ttttggggct
     1801 tatggtgatt acaagtcctg tgcgaagata tgtttgtagc aagatcagcg ttaatatttg
     1861 ttcggccaga gtctcgttta taaaggcagc agctgccaca atgttatatc tatcgatagt
     1921 attgtttgat attaaaggaa taatgattcc gcaatcgatt aatataatat gtacagtagt
     1981 gttgatatta ttgtttttat ctgaatcaat cggtattatt ataaaagtta tcaaaggaat
     2041 aataaatacg ctcgaatgtg atagaattag gatagcaaga ttgaaacacg atctttttct
     2101 gtcaataata gaggcattga tttctatttc gatattgttt tttttattta caggatttaa
     2161 tgacatatat gtatggtcag ctttaactct tttacttctt tttaacggct atgtggtttt
     2221 tgctacatgc agctacgagt ctgataatga taaagatgta gctagaaaag atgttgttga
     2281 cgagtcagaa gagcaactat ctgacgataa gaacagaata attagaggcc tttcagataa
     2341 ggccaaggca gatgagttgt ataaacgact tattatctat tttgaacaga aaaagccgta
     2401 tttacaacaa gatctcaaaa tcagagaggt cgccttatat ctctattcca acaagacata
     2461 cctttcacga atcattaatg acagacacaa tcttaatttt aatcagtttg tcaactacta
     2521 cagaattgag gaagttaaga ggctgtttag tgaaaatacc acactaagta ttcaggaatt
     2581 gtgtaccaga tcaggttttg gttctatggc aaccttttcg atagctttta gatatcattt
     2641 gggaaataca cctgcagact ggtgcaaaga gcagagactc ctcaaagaac atgaatagaa
     2701 atttaagatg cgtgataaaa agagttttat atttgtcagg tctttttgag aaagatcctg
     2761 ttttgaaatt tgtgaaatta tccgactctt tgttagttgg tggcaagaga gctgaagaat
     2821 tactgttaac caagcgatta tatttagatc cttgtcttac agttaccaaa cttgcaaaag
     2881 aagcagcaac aaacagaacc tatctttcaa attacatcaa tagcgagaag ggatgtaatt
     2941 ttagacaata cgttaatttg ttacgtgcag cgcaagctct taaacttatt gaatcagaaa
     3001 aaatggaaac actctcggat gttgcgatac aatccggatt tagtaattta agatcgctta
     3061 atagagcatt tattgctgtt tatggcaaac ttccctcaca ggtgagaagg gaatatctta
     3121 gggacaagca ttcataattc gttaattatg acttcctgtc taagatggga gcgaatgaag
     3181 ctttggcgat caggagtatt tttacccata tagaattcga gtagttgagg aatattgtca
     3241 tcattaccca gtcttaccct ttcgagtttt atgttatctc ctataaattc acggaactca
     3301 tctggcgaaa tctctcctag tcccttgaaa cgagtgatct ctatgttggc tccaagcttt
     3361 ttcattactt tgtccctctc ttctattgag tagcaataat gagtttcctt tttgtttctt
     3421 actctgaaaa gaggtgtctg taaaatatac aaatgtccct gacggataag ttccgggaag
     3481 aattgcagga aaaaagttag aaggagaagc cgtatatgca ttccgtcaac atcggcatca
     3541 gttgcaatta ttattttgtt gtatcttaaa ttatccagat ctgcttcaat attgagcgca
     3601 gcctgaagaa gattaaactc ttcattttca tacacaatct tctttgtaag tccgtaagag
     3661 ttttgaggtt tgcctctgag gctgaaaact gcctgagtag taacatctct gctttttgta
     3721 attgaaccac ttgcagaatc tccctctgtt atgaataggg tactctcttc agctctttca
     3781 ttcttatcgt tatagtgtat tctgcagtct ctgagttttt tgttatgcag atttgccttt
     3841 ttggctctct ctcttgcaag tttttgaatc cccgatatgg cttttctctc tttttccgaa
     3901 tccagtatct ttttaagtat ggtatcagcg gtctcgggat ttttatgcag gaaattgtcg
     3961 agattgtcct tcaaaaaatc gataatataa tttcgtatac tgactccgtt gacagacatc
     4021 tcttttgagc cgagcttagt ctttgtttgt ccctcaaact gtggttcctg tactttgata
     4081 cttattgcag caattattga ctgacggatg tcactcggat caaaatccct tttgtaaaac
     4141 tctttaagag ttttggcaat accttcacga aaagcggcaa gatgtgttcc tccttgtgta
     4201 gtgtactgac cgtttacaaa cgatgaaatg ctttctccgt agtcgtttcc atgggtcatg
     4261 gcaatctcta tatcctctcc tttcaagtga attggctgat aaagaggttc ttttgtaagg
     4321 ctttcattaa gaagatccag aagtccgttt ttggagaaaa actctttttc attaaatttg
     4381 atcgttaggc cggtatttag gtagcaatag tttctaagca tgctttcgac aaactcgaaa
     4441 ttgtatgaga agttttcaaa aagttcatca tcagccaaaa aagtgactaa agtaccgttc
     4501 ttctccgatg tagtgattct accttcttcg ataagttccc ctctgctaaa tttcgcaaaa
     4561 tgactttctc cctcccgaaa tgactgcaca agacatgaaa cggaaagagc atttacagct
     4621 ttaaggccga ctccattgag acccacagat ttcttgaagg cttcgtcgtc atatttgcct
     4681 ccggtgttta tcttgcttga tgcatctacc aggctgccga gaggaatacc gcggccaaaa
     4741 tctctaactg aaaccgtttt gtcttgaatt gtcacataga taattttgcc aaagcccatg
     4801 gcaaattcat ctattgagtt atccattacc tctttgagga gcacatatat tccatcgtca
     4861 ggcgaagagc catctccgag tttgccaata tacattcccg gtcttcttcg gatatgctca
     4921 ttccaattga gtgtacgaat atttttttct gtatatgtac ccatcttatc ttaatctctc
     4981 tttgcaacaa aggtataaat ttttaagcta aaaggtctgc aaagaggccg gatgctgtta
     5041 atcttgctcc tgctcctgct cccgagactg ttaatggaga agaatattca tcagtaaata
     5101 tcattattgc attatcacgg ccatttatat tataaaacgg atggtcagaa gagactgact
     5161 ggacaccgat tttataattg ccattttcaa ttgttgcaat gtaccttagc ctttcacctc
     5221 ttgagagtgc acttttatat aatgatagca tttggtcttc gcactcttct atctttgtca
     5281 taaattcctc tgaatcgtat gaaaaaagat cagaattaaa aataggtaca acatcaatct
     5341 cttccggttc aagtctaaat cctgattctc tggctaaaat tacagtcttt ctcaaaacat
     5401 cagttcctga aagatcaatt cttggatctg gttctgtgta accggcattt tttgcctcgc
     5461 gaacaatatc gacaaatttg gctgagccat catatttgct gaaaaggtaa ttcaaagtac
     5521 cggagaggat agcttcaatt cttcttatat tatctccaca ctcgactctg gacttgatag
     5581 ttgaaataac cggcaaagct gcacccacag ttgtctcata tttcaactta acttctgttg
     5641 cgcaacaagt ctcctgcaat gcatcataac tctcctggga tgaactaaga ccaatcttgt
     5701 tgcatgtaac gacagaacat tttccttgca taaaatcctg ataaagagaa gaaactcctc
     5761 tatccgctgt gcagtcaaca aatatactgt caggaagatt catgtctgag caaagttcac
     5821 gaaatctgta aatatcagat gggtcaccca ttttaagact ttcatctatt gagtctatct
     5881 ctattccact tttatttaaa atcatatgcc tgctattgca aatcgcaaca acatgtatct
     5941 ctaaatgtcg tttttctatg agccattctc tgcgagattt tattattgaa
//
LOCUS       DAOTQL010000105         5887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11008_length_5887_cov_5.025720, whole genome shotgun sequence.
ACCESSION   DAOTQL010000105 DAOTQL010000000
VERSION     DAOTQL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11008_length_5887_cov_5.025720"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1624)
                     /locus_tag="P5293_06530"
     CDS             complement(<1..>1624)
                     /locus_tag="P5293_06530"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRR49590.1"
                     /translation="FEQLLRKGFTQVRVDGSIHELVNLKPLDRYKNHFIELVVDKLIP
                     KEEDIVRIRESVSTALNMGKGSLALLEANGKELRFLSKQLMCPESGISFPEPAPHTFS
                     FNSPQGACPHCKGLGVIAKADISKIIPDDRKSIADGGIVPFGSMRENMLFAQLEGIAK
                     KYGFSLHSPIKEIPEEALNTILYGSEELFRVARGNSYTNMMTFSGVISRIEQNDSDSD
                     DTLERKERFIDEETCPECHGTRLRPESLCFKIDNLNIAEVSAMDIDLLKEWTLSLQEK
                     LKGKAALVAKDILKELQARIGFLVDVGLEYLSLDRAAKSLSGGESQRIRLATQIGSKL
                     VNVLYILDEPSIGLHQRDNIKLIDSLKKLRDEDNSVIVVEHDKEMILSADYLIDLGPG
                     AGEKGGRVLFAGAPGEIFSFGQDQIVKSQSLSLEYLTGMRKIDIPSQRRKGNGKFIEL
                     TGAAGNNLKSVDIKIPLGALIGISGVSGSGKSSLINETLMPILSNRLNHSRYTTLPYK
                     EIRGIENVDKVIEVDQSPIGRTPRSNPATYTNL"
     assembly_gap    1625..1724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1725..2276)
                     /locus_tag="P5293_06535"
     CDS             complement(<1725..2276)
                     /locus_tag="P5293_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRR49591.1"
                     /translation="MNDSIEVVGARVHNLKNINVSIPRNKLVVISGLSGSGKSSLAFD
                     TICAEGQRRYMETLSSYARQFMGVMERSDVESIAGLNPVIAIEQKSTNRNPRSTVGTI
                     TEISDFLRLLYAKASTAYSPVTGREMVKYTDEQIISLIIKNYNGEKTFLLAPLVKGRK
                     GHYKELFEQLLRKGFTQVRVDGSI"
     gene            complement(2273..3163)
                     /locus_tag="P5293_06540"
     CDS             complement(2273..3163)
                     /locus_tag="P5293_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRR49592.1"
                     /translation="MSIKLLLIIPLVAFCTGGEVYSERSSQDIEQQIVTIPELPDEIS
                     FAGEDVPLKYFDVRESLQREMIVITYWHSSLLLILQNDNRYVKEIKNILKQEGIPEDF
                     YYLCIAESGLQPVSSSAGACGYWQFLSSTAKEYGLIVDSEVDERYNIEKSTRAAAAYF
                     RKAYAELGSWTLAAASYNIGLSNVKYRKTIQHQNNYYDMQFPEETGRYLFRALAFKTI
                     MSDPQKYGYKVEKEQLYPELHYSEVKVNTPVENWSAFAARYNTNFKLLKMYNQWIKAN
                     YLTNKDRKTFVVRIPKEGTR"
     gene            complement(3153..3806)
                     /locus_tag="P5293_06545"
     CDS             complement(3153..3806)
                     /locus_tag="P5293_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRR49593.1"
                     /translation="MISTKTFYFNDLRECTYVVWDETGECVIIDPGCQTETERERLKK
                     FIENNKLKPVKLLNTHGHFDHVMGNAFVANTWGVKSYIHPLDKPQLERAYSYGEMFGY
                     NIEQPPVDTIDIEDGDEINFGNSCLKVITTPGHTRGGVCFYTEEDGNKIIFSGDSLFA
                     GSIGRTDLPGGDYDALMDSLLNKIIILGGDYKVLPGHGPETTISHELATNPFLSYEY"
     gene            complement(3830..4477)
                     /locus_tag="P5293_06550"
     CDS             complement(3830..4477)
                     /locus_tag="P5293_06550"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRR49594.1"
                     /translation="MLDTLQHQGRRKKLLDNLAKNFDFDTSVLEAMSRVPRHLFIEPG
                     LDHLAYLDRPLPIGAGQTISQPFTVAMQSHLLKAERFDKVLEIGTGCGYQTAVLAEMG
                     LRVYSIERQKSLYQLARRNLEKLGYHSPLLFLGDGFVGLPKFAPFKRILLACGAPEVP
                     SALINQLAVGGRMVIPVGTDVQKMLVVERLGENDFKTTEHGDYKFVPMLSGVEKK"
     gene            4553..5170
                     /locus_tag="P5293_06555"
     CDS             4553..5170
                     /locus_tag="P5293_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRR49595.1"
                     /translation="MHIAIAGNIGSGKTTLTRLLSKHYNWEAKYEDVDENPYLADFYN
                     DMQRWSFNLQIFFLKERFKGIVDIQKSSNNVVQDRTIYEDARIFATNLHDMGLMSTRD
                     FENYTSLFNLIMSLVKPPDLLIYLRSSIPNLVAQIHKRGREYENGIRIDYLSGLNERY
                     EKWIEEYRDGKVLIINVDEVKFEERPEDLSLVIDRIDALLHGLFK"
     gene            complement(5163..>5887)
                     /locus_tag="P5293_06560"
     CDS             complement(5163..>5887)
                     /locus_tag="P5293_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRR49596.1"
                     /translation="KASQIQEGINSILLKGRAASGISLVFITPPNFERTFAVYPGAAL
                     ELVPDDLTRDMFNGYDYLHIEGYLFQNHDLVLRAVEMAKSEGMIISLDMASYNIVESN
                     NNFLHSIVKDYVNIVFANEAEAETFAGCSPREALDILASMCDVAVVKIGKDGSMIKSG
                     KEYSFIRAKPAVTLDETGAGDLYAAGFLFAHSRGLSLDYCGEVGSIVAAAVVEVIGTK
                     IDMLRRELVKHKIRELTGLTFI"
BASE COUNT         1727 a         1286 c          975 g         1799 t
ORIGIN      
        1 aagatttgta taggttgcag gattacttct tggggttcta cctataggcg actgatcaac
       61 ctcaataact ttatccacat tttcgatacc ccgtatctcc ttatatggaa gcgtcgtata
      121 tctcgagtga tttaatctat tgcttaatat aggcatcagc gtttcattta tcaatgatga
      181 ttttccactt ccgcttacac cacttatacc gataagagct cccagcggga ttttaatatc
      241 cacactcttc aagttattac ctgcagctcc tgtaagttct ataaacttac cattaccttt
      301 tctcctttga gaaggaatat ctattttacg cattcctgta agatactcga gactcaaact
      361 ttgactcttg actatctgat cctgaccgaa tgagaaaatc tccccgggag ctccagcaaa
      421 taatacccga cctcctttct cccctgctcc cggaccaaga tctatcaaat aatctgcact
      481 taaaatcatc tccttatcat gctctacaac tatgaccgag ttatcctcgt cgcgaagttt
      541 cttaagagaa tcaatcaact ttatgttgtc acgctggtga agacctatac ttggttcatc
      601 aagaatgtac agaacattca caagttttga ccctatttgt gttgccagtc ttattctttg
      661 actttctcca ccactcaaag acttggcagc tctgtccaaa gagaggtatt caagcccgac
      721 atccaccaaa aaaccaattc ttgcctgcag ttcttttaaa atatctttcg caaccaatgc
      781 tgcctttcct tttagttttt cttgcaagct aagtgtccac tctttcaaca gatctatatc
      841 cattgcagat acctctgcga tgttcaaatt gtcaatctta aaacagagcg attcgggtct
      901 cagcctcgtt ccgtgacatt caggacatgt ttcttcatca ataaatcttt cttttctttc
      961 aagcgtatca tcactgtctg aatcattctg ctcaatcctt gaaattactc cagaaaaggt
     1021 catcatattt gtataggaat ttcctctggc tactctgaaa agttcttcag atccatataa
     1081 tatagtattt aatgcttcct ctggaatttc ctttatggga gaatgaagcg aaaacccata
     1141 tttttttgct atcccctcca attgagcaaa aagcatgttt tccctcattg aaccaaaagg
     1201 gacaatccct ccatcagcaa ttgatttacg gtcatccgga attatcttgc taatgtcagc
     1261 tttggcaatc actcctagtc ccttgcaatg aggacatgcc ccctgaggag aattaaaaga
     1321 aaaagtgtgt ggtgccggct cgggaaaaga gatgcctgac tcaggacaca ttaattgctt
     1381 gcttaaaaat cttaactctt tcccattggc ttcaagaaga gccaaagatc ctttccccat
     1441 gttaagagca gtggaaacag attcccttat tcgtacaata tcttcttctt tgggaatcag
     1501 tttgtcaaca acaagctcta tgaaatggtt tttatatctg tccagtggct ttaaattgac
     1561 aagttcatgg attgatccgt caactctgac ttgagtgaac ccttttctaa ggagttgttc
     1621 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngattga tccgtcaact
     1741 ctgacttgag tgaacccttt tctaaggagt tgttcaaaaa gttctttgta gtgtcctttt
     1801 ctgcctttta ccaaaggtgc aagaagaaaa gtcttttccc cattataatt ttttataatt
     1861 agagagataa tctgttcatc agtatatttt accatctcac gacctgtaac cggagaatag
     1921 gctgtagaag ctttggcata aagcagtctc agaaaatccg atatttcagt aatcgtacca
     1981 acagttgatc tcggatttcg gttagtgctc ttctgttcaa tagctattac aggatttaag
     2041 ccggcaatgc tctccacatc agacctttcc atcacgccca taaattgtct cgcataagaa
     2101 gagagtgtct ccatatacct tcgctgaccc tcagcacaga tagtgtcaaa ggcaagcgaa
     2161 gatttcccgc taccacttaa acctgaaata actacaagtt tattgcgtgg tatggagaca
     2221 ttgatgttct taagattgtg aactcttgcc ccaacaacct caatagaatc attcatcttg
     2281 tcccctcttt aggaattctc actacaaaag tctttctatc cttgttggta agataattgg
     2341 ccttaatcca ctgattatac attttgagta acttgaaatt tgtattatat ctggcagcaa
     2401 atgcagacca gttttcaacg ggtgtgttta cttttacttc actatagtga agctcgggat
     2461 aaagctgttc cttctcaacc ttatagccat atttctgagg atcactcatt atcgttttaa
     2521 aagcaagagc tctgaaaaga tatcttcctg tttcctcagg aaattgcata tcataataat
     2581 tgttctgatg ctggattgtt tttctgtact ttacatttga aagccctata ttgtatgagg
     2641 cagcagctaa agtccaggaa ccgagttcag catatgcctt tctaaaatat gccgcagctg
     2701 ctctcgttga cttctcaatg ttgtaacgtt cgtcaacctc actgtcaact atcagtccat
     2761 actctttggc tgtagaagat agaaactgcc agtagccaca tgcgccggca gatgaagaaa
     2821 ccggttgcag cccactctct gcaatacaca gataataaaa gtcctcaggg ataccttcct
     2881 gtttaagaat gttcttgatc tcttttacat aacgattatc gttctgcaag atcaataaaa
     2941 gagatgagtg ccaataagtt atcactatca tctccctttg cagagactct cttacatcaa
     3001 aatacttcaa aggaacatct tcccctgcaa acgaaatttc atcagggagt tcaggaatgg
     3061 ttacaatctg ttgttcaata tcttgactac ttctctcaga atacacttcg cctccggtac
     3121 aaaaagcaac gagaggtata atcaacaata atttaatact catagctcaa aaaaggatta
     3181 gtggcaagtt cgtgacttat tgtggtttcc gggccatgtc cgggaagaac cttgtaatca
     3241 cctccaagaa ttattatttt attgagtaaa gaatccataa gcgcatcata atctccacct
     3301 ggaagatctg tcctccctat acttccggca aaaagagaat ctcctgagaa aatgatttta
     3361 ttcccatctt cttcagtgta gaaacagacg cctcctctcg tatggccggg tgttgtaata
     3421 acttttagac atgaattgcc aaaattaatt tcatccccat cctctatatc tattgtatca
     3481 acaggaggct gttctatatt gtaaccaaac atctctccat atgaatacgc tctttcaagc
     3541 tggggtttgt cgagagggtg aatataactt ttcactcccc atgtatttgc cacaaatgca
     3601 tttcccatta catgatcaaa gtgaccatga gtatttagca attttaccgg tttaagtttg
     3661 ttattttcaa taaacttctt cagtctctct ctctctgtct cggtctgaca gccaggatca
     3721 attattacgc attctccggt ttcgtcccat actacatacg tacactccct gagatcatta
     3781 aaataaaaag ttttggtact tatcatatta taattatata aaatcgaatt tattttttct
     3841 cgacacccga taacatagga acaaatttgt aatccccatg ttcagttgtt ttaaaatcat
     3901 tctcccccaa tctttcaaca accaacattt tctgcacatc agtgccaaca ggaataacca
     3961 ttcttccccc tactgccagc tggttaataa gagcagaggg cacttcagga gctccacagg
     4021 caagcagaat ccttttaaaa ggtgcaaact taggcagtcc gacaaaacca tcacccaaaa
     4081 acaacaatgg ggaatgatac ccaagttttt caagatttct ccgagctaat tgatatagag
     4141 atttttgtct ttctatactg tatactctta gtcccatctc tgcaagaact gccgtctgat
     4201 atccgcatcc cgtacctatt tcaagcactt tgtcaaagcg ttctgcctta agcaaatgac
     4261 tctgcattgc aactgtgaaa ggttgtgata tagtctgtcc tgcacctata ggcaatggtc
     4321 tgtcaagata agcaaggtga tccagaccag gttcaataaa aagatgcctt ggcactctgc
     4381 tcattgcctc caagacagag gtgtcaaaat caaaattctt tgcaagattg tcaagaagtt
     4441 tcttcctcct tccctgatgc tgtagtgtat ctaacataaa tacaaaaata ctaaagattt
     4501 tttttaattt tgttttggag attgttatct ctccaataat aaataaacaa taatgcatat
     4561 agctatcgca ggaaatattg gcagcggtaa gaccaccctg accaggttac tttcaaaaca
     4621 ttataattgg gaggcaaaat atgaagatgt tgacgaaaat ccctatctcg cagatttcta
     4681 caatgatatg caaaggtggt ctttcaatct acaaatcttc tttcttaaag agagattcaa
     4741 aggaattgtt gacatacaga agagcagtaa caatgtcgtt caagacagaa ctatttacga
     4801 agatgctcga atatttgcca caaatctgca tgacatgggg cttatgagca ctcgagattt
     4861 tgaaaactat acttcccttt tcaatttaat aatgtctctc gtaaaaccac ctgacttatt
     4921 gatatatctc aggtcctcca ttcccaatct cgttgcacag attcataaaa gaggacgtga
     4981 gtatgaaaac ggcatcagaa tagattatct atccggatta aacgagcgtt acgaaaaatg
     5041 gattgaagaa tatagggacg gaaaagtttt gataatcaat gtggatgaag tcaaatttga
     5101 ggaaagacct gaggatttaa gcctcgttat tgaccggata gatgcattgc ttcatggtct
     5161 gttcaaataa atgtcagacc tgtaagttct ctgattttat gtttgacaag ttccctgcgt
     5221 agcatatcaa ttttagtacc tattacttct acaactgcag ctgcaactat tgagccgact
     5281 tcaccacaat aatcaagcga taaaccacgg gagtgagcaa ataaaaaacc tgcagcatac
     5341 agatctcctg caccagtttc atcaagagta acagcaggtt ttgccctgat aaagctatac
     5401 tcttttccac tttttatcat tgaaccgtct ttgcctattt tgactactgc aacatcacac
     5461 atggaagcta aaatatcgag agcttcccgc ggtgagcatc cggcaaatgt ttcggcctca
     5521 gcttcattgg caaaaacaat attcacataa tccttaacaa tactgtgcaa aaaattatta
     5581 ttactctcca caatgttata gcttgccata tcaagagaga taatcatgcc ttcagattta
     5641 gccatttcaa cagccctaag gacaagatcg tgattttgaa acaaataacc ctctatgtgt
     5701 aaatagtcat aaccattaaa catgtctcgg gtgagatcat ctgggaccag ttccaacgct
     5761 gccccgggat aaacagcaaa agttctttca aaatttggag gtgtgataaa tactaatgag
     5821 ataccagaag cagccctgcc tttcaaaaga atggaattaa tcccttcttg tatctgactt
     5881 gctttaa
//
LOCUS       DAOTQL010000106         5668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11477_length_5668_cov_4.752361, whole genome shotgun sequence.
ACCESSION   DAOTQL010000106 DAOTQL010000000
VERSION     DAOTQL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11477_length_5668_cov_4.752361"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="P5293_06565"
     CDS             <1..212
                     /locus_tag="P5293_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49597.1"
                     /translation="RSNVQYGGTGFFFDKAEPLPYEIEHHMPDYHLYDDWVKLEVDRG
                     KKNKQVEILYEFLYWVYDKGMLSWM"
     gene            184..918
                     /locus_tag="P5293_06570"
     CDS             184..918
                     /locus_tag="P5293_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49598.1"
                     /translation="MTRGCFRGCEFCVNRNKTRVEFHAHLKEFLDPTLKGISLLDDNI
                     LGYPRWREVFDELIASNKPFEFKQGMDIRLLTKEKAKVISSCRYHGNYIFAFDNIEDK
                     ELIIKKLQIWREIVEPRTRTVLYVLCGFDKTGKYNDVFWRQDLINMWEKIKILMTYGC
                     APYIMRFNKCEGPDKEFYSLVRGWCNTVHNFIGKSFREYCEHPAKDANVNYMPRFREI
                     EKKYPEIAKKYFDLKFEREVIDRGWW"
     gene            1013..1594
                     /locus_tag="P5293_06575"
     CDS             1013..1594
                     /locus_tag="P5293_06575"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR49599.1"
                     /translation="MAEAKTIIIDVGHTKQSPGVISTIGESEFIYNKIFGITLMWKLK
                     RDGHDVTIIENQSLKERVRNINSSDADLVISIHHDSTQPQYLGKNKYRGFSIFIHDKK
                     NLPFAMDLGKRLVECGYSPSLHHAENILGERKPLLSKEYGVYRSNFYILKHSKKPIVL
                     FEVGVITNSEEEKTLRDSSSIDKMAQCIVEVLK"
     gene            1596..2210
                     /locus_tag="P5293_06580"
     CDS             1596..2210
                     /locus_tag="P5293_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HRR49600.1"
                     /translation="MSAIINFFDIEKNINTKPVLYLVKTDKEKRICKSIIEKYHSYVP
                     SFKSVGRRIDWLIEYQDELVGMIGIGSSTYPPPKDLLRYLNISKEEYRKTFNSFANNW
                     RFCLIKKIPNLGTMVLKEMRCNAGIEWKKKYNNDLHYIITFVGEGRDGAVYKADNWVR
                     IGETSGLPKHKSVSMKWDDNKSLKNKFVKPTGENKKMIFIKKIV"
     gene            2785..3051
                     /locus_tag="P5293_06585"
     CDS             2785..3051
                     /locus_tag="P5293_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49601.1"
                     /translation="MKKVFHSKTHQTKSNRKQTKKIRKKEKRMNKRETFKYVIEILEG
                     EIVNKTLKMSIIGKTLVEEKISNRENILKVIRVFRKRGIEIVRG"
     gene            3087..3380
                     /locus_tag="P5293_06590"
     CDS             3087..3380
                     /locus_tag="P5293_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49602.1"
                     /translation="MRLPTNIKKKINRKISDLTKVTHKSIPLGIIDSFLRENGFFLIQ
                     EDGQPWSGFLLGENSQATIDIGSENDIVTNAVLVISWYRYSITGRYEIVAYIS"
     gene            3392..3607
                     /locus_tag="P5293_06595"
     CDS             3392..3607
                     /locus_tag="P5293_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49603.1"
                     /translation="MKESTYRGLVKVIERLDSVQKKIRNSFNVSETNLDIKDAREYGY
                     ISSSIKDLEDITDELKTIRSLFFLKDE"
     gene            3787..3978
                     /locus_tag="P5293_06600"
     CDS             3787..3978
                     /locus_tag="P5293_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49604.1"
                     /translation="MKEIKLIISDDVYRDLKNALTVKWLSQMAGQDVTLAVIEKILRS
                     LDNNEETCELIYKDRKKGK"
     gene            3980..4522
                     /locus_tag="P5293_06605"
     CDS             3980..4522
                     /locus_tag="P5293_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49605.1"
                     /translation="MKILDMKVCWYENFDNDPQLQILVDKMPDFDDLLYEKKDGLYFA
                     EKDGYVSFFVYTKPGEGFGGRVFPITLTDGTKVDLKGPWSSRAGVMNKAGFEPCLDVH
                     LTDDPEVWKRGYTFYAAHVTKKLVDEYLDKFLPDVFLYKQEDKRDGDIIYLPYLKDGR
                     KKPNPQTYWGKRRNLWREVE"
     gene            4759..4992
                     /locus_tag="P5293_06610"
     CDS             4759..4992
                     /locus_tag="P5293_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49606.1"
                     /translation="MKNECGKTRDIKNPYEIWVNNQGWEWRVLKKYQTPEKEMKNPYA
                     RWFCAVKSPFTYGSYEYGDTYVKDIITYAYRVK"
     gene            5002..5241
                     /locus_tag="P5293_06615"
     CDS             5002..5241
                     /locus_tag="P5293_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49607.1"
                     /translation="MNKPNVWLSGYVIGVKVVDVMEQKDRDLFREDLWDSFRNLISVE
                     ENFDSYILEFEDVGDIDPKELIDKIERIQAKYLKE"
     gene            5379..5558
                     /locus_tag="P5293_06620"
     CDS             5379..5558
                     /locus_tag="P5293_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49608.1"
                     /translation="MDVLEKEMLELVEEHTQKILRENFDPKIHLKLRGILQKSVLRGV
                     SIGILWERREKNGRS"
BASE COUNT         1961 a          828 c         1287 g         1592 t
ORIGIN      
        1 aaagatcaaa tgttcaatat ggtggaactg gttttttctt tgataaggct gaacctctcc
       61 catatgaaat tgagcatcat atgccggatt atcacctata tgatgattgg gtaaagttgg
      121 aagtggatag aggaaaaaaa aataagcagg ttgaaatact ttacgagttt ctctattggg
      181 tttatgacaa ggggatgctt tcgtggatgt gagttttgtg taaatagaaa caaaaccaga
      241 gttgaatttc acgcacacct taaagaattt cttgatccta ctctcaaagg aatatcgcta
      301 ttggatgata acatccttgg atatccaagg tggagagagg tatttgatga attgattgcc
      361 tcaaataaac catttgagtt taaacaggga atggatatcc gcttactgac aaaagagaag
      421 gcaaaggtaa tatcctcctg tagatatcat ggtaattata tttttgcgtt tgataatatt
      481 gaagataagg aattgattat aaagaaactc caaatatgga gagagatagt tgagccaaga
      541 acaagaactg tattatatgt tctttgtggt tttgataaaa cggggaaata caatgatgta
      601 ttttggagac aggacttgat taatatgtgg gaaaaaataa aaattcttat gacatacgga
      661 tgcgcaccat atattatgag attcaacaaa tgtgaagggc cggacaaaga attttatagt
      721 ctggtaagag gatggtgtaa taccgttcat aacttcatcg gtaaatcgtt tcgggaatac
      781 tgtgaacatc cagcaaaaga cgctaatgtg aattatatgc ccagatttcg tgagatagaa
      841 aagaagtatc ccgaaatagc taagaaatac tttgatctga aatttgagag ggaagtaata
      901 gatagaggat ggtggtaaaa cattgacaag aattataaaa caaagtatca tttggataat
      961 cggtagtctg ttcattttcc cgctttgttc attttctgtg aacacggatt ctttggcaga
     1021 agctaagacc atcatcattg atgtgggtca caccaagcaa agtccgggcg tgatttccac
     1081 catcggggaa tctgaattta tctataacaa gatatttggt ataaccctta tgtggaaact
     1141 caagcgagat ggtcacgatg taacaatcat agaaaaccaa tccctgaaag agagggtgag
     1201 aaacatcaat tcatcggatg ctgatttggt tatatccatt caccatgata gtacgcaacc
     1261 ccagtatttg gggaaaaaca agtatcgggg tttctccata ttcatccacg ataagaaaaa
     1321 cctgcccttc gcaatggatt tggggaagag attggtggaa tgtgggtatt caccatcact
     1381 ccaccacgcg gaaaacattc tgggcgagag gaaaccgctc ttgtcaaagg agtatggtgt
     1441 ttatagaagc aacttttaca tattgaaaca ttccaagaag cccatcgtgt tgtttgaagt
     1501 aggtgttatc acaaattcgg aagaggaaaa gaccttgcgg gacagttcat caatagataa
     1561 aatggctcaa tgtatcgtag aggtattaaa ataaaatgtc cgcaataatc aatttctttg
     1621 atattgaaaa aaatatcaat acaaagccgg tcctttatct tgtcaaaaca gacaaagaaa
     1681 aaagaatctg taaatctatt attgaaaagt atcattctta tgttccttcc ttcaagtccg
     1741 ttggtaggag aattgattgg ttgatagaat atcaagatga attggtaggt atgataggta
     1801 ttggcagttc tacttatcca ccaccaaagg atttactgag atatttgaat atctcaaaag
     1861 aagagtatag aaagacattc aactctttcg ctaataattg gagattttgt cttataaaaa
     1921 agattccgaa tcttggcaca atggttctca aggaaatgag atgtaatgct ggaatcgagt
     1981 ggaagaaaaa atataataat gatctacatt atattatcac ttttgttgga gaaggccggg
     2041 atggtgctgt ttataaagcc gacaattggg taaggatagg ggaaacatcc ggtttaccga
     2101 aacataagag tgtttcaatg aaatgggatg ataataaatc gctaaaaaac aagtttgtga
     2161 aacctaccgg agagaataaa aagatgattt ttatcaaaaa aatagtgtaa aataccgtat
     2221 cttgactttt ttgccctttt tgctgataat ggaatcgaaa ggaggtgatt ctgaaatggc
     2281 cgacttaccg gattccttag aatataggct atccgatgaa ggcctcgtct tcgcggccga
     2341 cacaaagtcg ctcaagacct tcgcggtctc gggcgatagc ttggtcgaga ttttggatga
     2401 ccatacgtca tacaagatct tgtataggat gtcccggctt tccaaaccgg aagcactggc
     2461 cttgttgaaa gcctcccgac aattgggtaa ggatagggga aacatccggt ttaccgaaac
     2521 ataagagtgt ttcaatgaaa tgggatgata ataaatcgct aaaaaacaag tttgtgaaac
     2581 ctaccggaga gaataaaaag atgattttta tcaaaaaaat agtgtaaaat accgtatttt
     2641 ttgcgttttt tgaccatttt tgaccctaaa aatgacttaa atatccgatt ttattgagaa
     2701 aaatagtgaa aaataatcct tgacataagc accgaattat ggtagaatta ttatggaaaa
     2761 attgaaaagg ataaaggtta ggaaatgaaa aaagtcttcc atagtaaaac tcatcaaacc
     2821 aagtccaatc gtaaacaaac caaaaagatc agaaagaagg aaaaaagaat gaacaaaaga
     2881 gaaactttca agtatgttat tgagatttta gaaggtgaaa ttgtcaacaa gaccttgaaa
     2941 atgtccatca tcggaaaaac tttggttgaa gagaaaattt ctaacaggga aaacatcttg
     3001 aaggtcatca gggttttcag aaaaagggga attgaaatcg taagaggata ggaaataaga
     3061 ttttatgtaa aaggaaaaga aataaaatga gactgcccac aaatatcaag aagaaaatca
     3121 ataggaaaat ttctgatctg acaaaagtaa cccacaaatc aattccgtta ggcatcattg
     3181 atagtttctt gagagagaat ggtttttttc tcattcaaga agatggtcag ccttggtcgg
     3241 gatttcttct cggtgaaaac agccaagcca caattgatat tggaagtgag aatgatattg
     3301 ttaccaatgc cgttcttgtc atttcttggt atcgttactc cataaccgga cgatatgaaa
     3361 tcgtcgctta catctcgtaa tgaggtgaga tatgaaagaa tctacttata gaggattagt
     3421 taaggtaatt gagagattgg atagtgtcca gaagaaaatc agaaattcgt tcaatgtttc
     3481 tgaaactaat ctggatatca aggatgctcg tgaatatggt tatatttcct catccataaa
     3541 ggatttggaa gatattacag atgagttgaa aaccatcaga tcacttttct tcttgaagga
     3601 cgagtaatat gtgtgataag tgccacaaaa gaaaaccgtt gcggccacct tgtccaagga
     3661 cattgaagaa gctcgacccg cgtgagtgct ggtatggatg ccatacacat tacaagggtc
     3721 acccttgtaa atcatattta gaatatgtaa aacggcttcg cgctagagag gataggagaa
     3781 agtaagatga aagaaataaa acttatcatt tccgatgatg tatatcgtga cttgaaaaat
     3841 gcgctaacgg taaagtggct gagccagatg gcgggccagg atgttactct ggctgtgatt
     3901 gaaaagattc tcagatctct ggacaataat gaagagactt gtgagttgat ctataaagat
     3961 cggaagaaag gaaagtaata tgaaaatttt ggacatgaaa gtttgctggt atgagaattt
     4021 tgacaacgat ccccagctcc agattttggt ggataagatg ccggactttg atgatctact
     4081 gtatgagaag aaagacggcc tctattttgc agagaaggac gggtatgttt ccttttttgt
     4141 ctacaccaag ccgggtgagg ggtttggtgg aagagtattc cctattactc ttacggatgg
     4201 caccaaggtc gatctaaaag gcccgtggtc aagcagggcc ggtgttatga acaaggcggg
     4261 gtttgagccg tgcctcgatg ttcatcttac ggatgatccg gaagtgtgga agaggggata
     4321 tactttctat gccgctcatg ttacgaagaa actggttgat gagtatctcg acaagtttct
     4381 tcccgatgtt ttcctctaca agcaagaaga caagagggat ggggatatca tttatctgcc
     4441 ctatctgaaa gatggcagga agaaaccaaa tcctcagacc tattggggta aacggagaaa
     4501 cctttggaga gaggtggaat aaaatgagtc ataagtcata cgaaaagaag gatatgccaa
     4561 aactcaagcc gtttttctga attgactaat gattgggtat atgcaagata cataattctc
     4621 tatgaccaca aaggtaatga atataggatg gacaaaaggc taatactcaa tttcttgaat
     4681 tttcattcag atgatttggt catggtcaag aaaagaaaac ataaccattt gagatggtta
     4741 gaaagtgagg aataaaatat gaagaatgaa tgtggaaaga caagagacat taaaaatcct
     4801 tatgagattt gggtgaacaa tcagggatgg gagtggagag ttctcaagaa atatcagacc
     4861 cctgaaaagg aaatgaagaa tccttatgcg agatggttct gtgctgtaaa atctccgttt
     4921 acttacggtt cctatgagta tggtgatacc tatgtgaaag atattatcac ttacgcttac
     4981 agagtgaagt aagaggtgga tatgaataaa cccaatgttt ggttgtctgg atatgtaata
     5041 ggggtcaagg ttgttgatgt gatggaacag aaagaccgag acctgttcag agaggatttg
     5101 tgggattctt tcagaaatct catttctgtg gaggaaaact ttgacagtta tattcttgaa
     5161 tttgaggatg taggtgatat tgatccgaaa gagttgattg acaaaataga gagaattcaa
     5221 gcaaagtatt tgaaagaata ggatgtgagg gaaaaagaaa tgaaaaagtt aaatgatggt
     5281 cttgattgta ttcgtggatg tatggtagct tttccatttt gtcttgtttt ctggattatg
     5341 gttgtcattc tcatttatta ttttatgagg aattgattat ggatgtattg gaaaaagaaa
     5401 tgcttgaatt ggtcgaggag catactcaga aaatattgag agaaaatttt gacccgaaaa
     5461 tacacttgaa actccgtggt attttacaaa agtctgtatt gagaggagta tcaatcggta
     5521 tattatggga aaggagagaa aaaaatggac gatcataagt ggaaagtagt tatgatgttt
     5581 aatgatgaac caaaacaggt tgaggaaatg atcctgacaa agccggagtt gtgggagaga
     5641 ttcagaaaat atatccccgc catcgaaa
//
LOCUS       DAOTQL010000107         5665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11488_length_5665_cov_4.810517, whole genome shotgun sequence.
ACCESSION   DAOTQL010000107 DAOTQL010000000
VERSION     DAOTQL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11488_length_5665_cov_4.810517"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1369)
                     /locus_tag="P5293_06625"
     CDS             complement(140..1369)
                     /locus_tag="P5293_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRR49609.1"
                     /translation="MKKIKIICIVFLSLSALRGYSQKVMTGLEVLKASNFKILEGKRV
                     GLITNPTGFDNNIKSTVDILFEAPNVKLVALFGPEHGVRGNAHAGDRVEASTDPKTGL
                     PVFSLYGDTRKATPEMLKGIDVLVYDIQDIGCRSFTYISTMGLAMEAAAENNIEFVVL
                     DRPNPLGGLKVEGNVVEDGFISFVSQFKIPYVYGLTCGELATLLNEEDMIKGHCKLTV
                     VPMKGWKREMTFDQTGLQWVPSSPHIPQPISAICYPVSGILGELGYLSIGVGYTIPFQ
                     MFAAEWIDAEELAARLNSYKLPGVRFRPMYLKPFYSVGAGKDFQGVQVHILNYQTCAL
                     TDIQFYVMQAVAELWPEKAVFNNADVKRFNMFDKVSGTDVIRKKFSERNRFEDIKDYW
                     YKDVENFKKLSKRYYIY"
     gene            complement(1366..3231)
                     /locus_tag="P5293_06630"
     CDS             complement(1366..3231)
                     /locus_tag="P5293_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR49610.1"
                     /translation="MSNKKRSPWAWIPTLYFAEGLPYVAVMTVSVIMYKRLGISNADI
                     ALYTSWLYLPWVIKPFWSPFIDLLKTKRWWVVTMQFLISVGLAGVAFTIPASNFFQLT
                     LAVFWLIAFCSATHDIAADGFYMLALDSHDQAMYVGIRSTFYRVATITGQGILVIIAG
                     LLEMSTGLQPAEFKVNAGPQYTSSFVIPQAEETVISSATGEDDYKFILNPSVVNLSTR
                     TVAVDSAAILKAYALENNIANGFVEPEKSAGNVKKEASWWTEHVSTPLGAFIEENFGE
                     KNSGKVVHEMNGNAQLVTVRLNKAPASGEKIVLNTAMKKGDKSIFIAHGERITFTSEN
                     WDKPAYILVQADPKLDHETSTVFRGLSGNIPLAWSVTFFILAGFFLAIAVYHRYILPR
                     PDTDKPAREVTAKTIFKEFFATFASFFKKKQIWVAILFMLLYRLPEAQLVKLITPFLL
                     DAREVGGMGLTTGQVGLVYGTVGILALTIGGIIGGIVAAKGGLKKWLWPMAWSISLTC
                     ITFVYLSIFQPENFFVVNLCVFIEQFGYGFGFTAYMLYLIYFSAGEHKTAHYAICTAF
                     MALGMMLPGMMAGWLQELVGYRNFFWWVMICCLATIIVTAFIKVDESFGKKEVKA"
     gene            complement(3242..4561)
                     /locus_tag="P5293_06635"
     CDS             complement(3242..4561)
                     /locus_tag="P5293_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRR49611.1"
                     /translation="MTEPQVTVGIMFEPKIEFKLNGLFICNNLKIEGPQTVNFCNGKI
                     EWMGDLFEELLFEPEDRQSDSFELIGVTIGINFHWERKENQTFRGSLKFIVENEKITA
                     INIIEVEEYLTSVISSEMSATASLELLKAHAVISRSWLMAQIQKNRDISNSQKIYSTV
                     HDTPRELIKWYDREDHIRFDICADDHCQRYQGITRASTEIVKDAIAQTRGQLLMYGGK
                     ICDARYSKSCGGVLEEFGSCWEDINYPYLVKKRDSMTHTEVPDLTIELNAREWILSSP
                     EAFCNTTDQKILSQVLNNYDQETVNFYRWSVTYTQHELSALIKKRSGTDYGEIIDLLP
                     VQRGTSGRLVKLKIVGTKRTRIIGKELEIRRTLSDTHLYSSAFIVSRLDVDNKGIPGR
                     FILKGAGWGHGVGLCQIGAAVMGEKGYKYNEILLHYFVGASIEKLYL"
     gene            complement(4572..5522)
                     /locus_tag="P5293_06640"
     CDS             complement(4572..5522)
                     /locus_tag="P5293_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HRR49612.1"
                     /translation="MSELNSEMVNRLFEEQLSSWKQARDNYEALDRVMTREVNVDGFT
                     FKIQFNPARIVSSAAKVDAKSIKERNCFLCKENRPAVQKGIDFAFDDKDHYTVLINPF
                     PIFKRHLTIPLEEHRDQLIRGRFNVMLEFARRLTDFTIFYNGPKCGASAPDHFHFQAG
                     IKGFLPIESSHLNTKTFFSDSEITLEQVVSGLPGIIIMESSSGSKAEDFFDNLYSSLE
                     IKEGEREPMLNILCSYEKGKWRIVFFLRKKHRPDAFFKEGDENILISPASVDLGGVLI
                     TPLEKDFVKLTAKDINSILSEVLISYDELETIVKKLKLII"
BASE COUNT         1729 a         1278 c          935 g         1723 t
ORIGIN      
        1 aagctcgtca gcggtcaaag tctcgactat agactttttc aatgggtttg agcagctgat
       61 tgctacagtt gctacgaaaa ggagtaaaaa tagctttttc ataaccaata agtttttctc
      121 aatataaagc tacacatttc taataaatgt aatatctctt tgaaagcttt ttaaagtttt
      181 caacatcttt gtaccagtaa tcctttatat cttcgaatct gtttctttct gaaaattttt
      241 tacggataac atctgtgccg gaaactttgt caaacatatt aaatctcttg acatccgcat
      301 tattaaaaac agctttttcc ggccaaagtt cggcaacagc ctgcatgaca taaaactgaa
      361 tatctgtaag cgcacaagtc tgatagttaa gtatatgaac ctgaactcct tgaaaatctt
      421 ttcctgctcc tacagaataa aaaggtttaa gatacatagg tctaaatctc acaccgggca
      481 atttgtaact gttcaacctt gccgcaagtt cctctgcatc tatccactct gctgcaaaca
      541 tttgaaaagg aattgtataa cccacaccta tagaaagata tcccaactcc ccaagtattc
      601 ctgagacagg gtagcaaatt gcactgattg gttgaggaat atgaggtgac gatggtaccc
      661 attgaagtcc tgtctgatca aaagtcatct ctctcttcca tcctttcata ggtactactg
      721 tcagcttgca atgacctttt atcatatcct cctcattgag caaggtggca agttcaccgc
      781 aagttaaccc ataaacgtat ggtattttaa attgactgac aaatgagata aacccatctt
      841 ccactacatt gccttctacc ttcaggccgc caagaggatt aggcctgtca agaactacaa
      901 attcgatatt attttcagca gcagcttcca tagccagtcc cattgtactt atataggtaa
      961 aagatctgca tcctatatct tgtatgtcat ataccaatac atctatcccc ttaagcatct
     1021 ctggcgtagc ctttctggta tccccataga gagaaaaaac aggtaatccg gttttagggt
     1081 ccgtgcttgc ctctacccta tcgccagcat gagcattacc tcttactccg tgttcaggcc
     1141 caaatagggc taccagttta acattaggag cctcaaacaa gatatctaca gtcgatttta
     1201 tattattgtc aaaccctgtt ggatttgtta taagaccaac tcttttcccc tcaagtattt
     1261 tgaaatttga agccttgaga acttccaaac ccgtcattac cttttgagaa tatcctctca
     1321 atgcagagag agagaggaaa acaatacata ttatttttat ttttttcatg ctttaacttc
     1381 ttttttacca aaggattcat ctactttaat aaaggctgtt acaataattg tggccagaca
     1441 gcatatcatc acccaccaaa agaaatttct atatcctaca agttcctgca accagccagc
     1501 catcatacct ggcaacatca tgcccagtgc catgaaagcc gtacatatag cataatgtgc
     1561 cgtcttgtgt tctccggcag agaaatatat caaatacagc atataggccg tgaaaccaaa
     1621 accatatcca aactgctcaa taaagacaca aagattgact acaaaaaagt tttcaggctg
     1681 gaatatgctc aaatatacaa atgtgataca agtaagcgag atactccacg ccatcggcca
     1741 aagccacttt ttaagtcctc ccttggcagc aactattcca cctataattc cgccaattgt
     1801 cagagccaag atgcctacag taccataaac aagacctacc tggcctgtgg taagtcccat
     1861 ccctccgacc tctctggcat caagcaagaa tggagtaatc aatttaacca gttgagcttc
     1921 agggagacga tagagtaaca tgaacaaaat agcaacccat atctgttttt tcttgaagaa
     1981 agaggcaaat gttgcgaaaa attccttgaa aattgtcttt gctgtaactt ctctggctgg
     2041 tttgtccgta tcaggccttg gaagaatgta tctatgataa actgcaattg caaggaaaaa
     2101 tcctgccaaa atgaaaaaag ttacagacca tgcaagggga atgtttccgg aaagtcctct
     2161 aaagacagtc gatgtctcat gatcaagttt aggatctgcc tgtaccaata tatatgcagg
     2221 tttgtcccag ttttctgagg taaatgtaat tctctcacca tgagcaataa aaatgctttt
     2281 atcacctttt ttcatggcag tattcaacac gattttttct ccacttgcag gagccttgtt
     2341 tagtctgaca gtcacaagtt gggcattccc gttcatctcg tgaacaacct ttccactgtt
     2401 tttttctcca aaattttctt caataaaagc gcctaaaggt gttgagacat gctctgtcca
     2461 ccatgatgcc tcttttttta cattacctgc actcttttct ggctccacaa aaccattcgc
     2521 aatgttgttt tctaaggcat atgccttaag aatggcagcc gaatctacag caacagttct
     2581 tgtacttaaa ttgacaacag atggattcaa aataaatttg taatcatctt ctcctgttgc
     2641 agatgagatt acagtctcct cagcctgagg aataacaaaa gaagaggtgt attgaggccc
     2701 tgcattaact ttaaactctg ccggctgcaa acctgttgac atttcgagta aaccggcaat
     2761 aataaccagt atcccctggc ctgtaatagt ggcaactctg taaaaagtac ttctaatccc
     2821 aacatacata gcctgatcat gactgtcaag cgcaagcatg taaaagccat cagctgcaat
     2881 atcgtgcgtt gcagagcaaa aagcaatcag ccaaaatacg gcaagagtca gctgaaagaa
     2941 attgcttgca gggatagtaa acgcaactcc tgcaagtccc acacttatta agaattgcat
     3001 cgttaccacc caccatcgtt tagttttaag caaatctata aaaggactcc aaaatggttt
     3061 tataacccaa ggtaaataga gccagctcgt atataaagcg atatcagcat tagaaatacc
     3121 taatctcttg tacatgatca cagagacagt cattactgca acatatggca gcccctcggc
     3181 aaaatacaga gtaggtatcc acgcccaggg agatctcttt ttgttactca taatttaaat
     3241 attatagata taatttttcg atagatgcac ctacaaaata atgaagtaaa atttcattgt
     3301 acttataacc tttctctccc atgacagctg ccccaatttg gcaaagtccc actccatgac
     3361 cccatcctgc tcctttgagt ataaaacgtc caggaattcc cttattatct acatcaagcc
     3421 tcgatacaat aaatgctgaa gagtaaagat gtgtatcgga aagtgttctc cttatctcaa
     3481 gttctttccc aataattctt gttctttttg tccctacaat tttaagcttc accagacgcc
     3541 ccgatgtacc cctctggact ggcagaagat caataatctc accataatca gttccggaac
     3601 gtttctttat gagtgcagac aattcatgct gtgtataggt cacgctccac ctgtaaaaat
     3661 tcacagtctc ctgatcatag ttattcaata cctgagacaa tatcttttgg tcggttgtat
     3721 tgcaaaaagc ctccggtgaa gataaaatcc actctcttgc gttaagttct atggttagat
     3781 cgggaacttc agtatgtgtc atactgtctc gctttttgac cagataggga taatttatat
     3841 cttcccagca actgccaaat tcttcaagaa ctcctccaca acttttggaa tagcgtgcat
     3901 cacatatttt acctccgtac atgagcaact gccctcttgt ctgtgcaatt gcatctttca
     3961 caatctctgt tgatgccctg gttatcccct gataacgctg acaatgatca tccgcacaaa
     4021 tgtcaaatct aatgtgatct tctctgtcat accattttat caattctctc ggagtatcgt
     4081 gaactgtaga ataaatcttt tgtgagttgg agatgtctct gttcttctga atctgagcca
     4141 taagccacga tcttgaaatg acagcatgag ccttgagcag ttccagagaa gctgtagcac
     4201 tcatttcaga tgatataacc gaagtaagat actcttcaac ctcaataatg tttatcgcag
     4261 ttatcttttc attttcaaca ataaatttga gagaaccacg gaaagtctga ttttcctttc
     4321 gttcccaatg gaaattaatt ccaattgtaa ccccaattag ctcaaaagaa tcgctttgtc
     4381 tgtcttcagg ctcaaataac aattcttcaa acaaatctcc catccactcg atcttgccat
     4441 tacaaaaatt gacagtttga ggaccttcga ttttgagatt attacaaata aacaatccat
     4501 taagtttgaa ctcgatcttg ggttcaaaca ttattcctac agttacttga ggttctgtca
     4561 tatgagtatt gttaaataat taatttcaat ttcttgacaa ttgtctcaag ttcatcatat
     4621 gatatgagaa cctccgataa aattgagttg atatctttag cggttagctt gacaaaatcc
     4681 ttctcgagag gagtaatcaa aactccacca agatctactg aagcgggact gatcaatata
     4741 ttttcatctc cctccttaaa aaaggcatcc ggacgatgtt tcttccttaa aaaaaacact
     4801 atcctccatt tgcctttttc gtaactgcat aaaatattaa gcataggttc tctttcccct
     4861 tctttaattt caagtgaaga gtataaattg tcaaaaaaat cttcagcctt gcttcctgac
     4921 gaggattcca taattataat tcccggcaac cctgaaacta cctgttcaag ggttatttcg
     4981 gaatcagaaa aaaatgtttt ggtattaagg tgagaactct caattggcaa aaaacctttt
     5041 atccccgctt gaaaatgaaa gtgatcagga gctgaagcac cacattttgg cccattgtaa
     5101 aaaattgtaa agtcagtcaa ccttcttgca aactcaagca taacattaaa tcttcctctg
     5161 ataagttgat cacggtgttc ttcaagtgga attgtaagat gtcgcttaaa aataggaaat
     5221 ggattgatta gaacagtata atgatcctta tcatcaaacg caaaatctat ccccttttgt
     5281 acagcaggtc tgttttcttt acataaaaag caattacgct cttttatgga ctttgcatct
     5341 accttggctg cagaagatac aattcgtgca gggttaaact gtatcttaaa cgtaaaacca
     5401 tcaacattga cctctctggt catgactctg tcaagtgcct cataattatc acgagcctgt
     5461 ttccaggagc taagctgctc ctcgaaaagc ctattaacca tctcagaatt taactctgac
     5521 atccaactat ttccctcttt tgttcatagc gattctggcc tgaagctccc aagttcttat
     5581 cctatccttg taggtgttgt ttgcgttcat ccttactata tcgagggcag catcagagtt
     5641 tccttcccaa cgtcggcaat gataa
//
LOCUS       DAOTQL010000108         5611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11624_length_5611_cov_5.479122, whole genome shotgun sequence.
ACCESSION   DAOTQL010000108 DAOTQL010000000
VERSION     DAOTQL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11624_length_5611_cov_5.479122"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="P5293_06645"
     CDS             <1..1009
                     /locus_tag="P5293_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRR49613.1"
                     /translation="LPLSHAYECSIGMIQPFTHGAHVVYLDGSPTPSLLLPAINDVRP
                     TIMLTVPLIIEKIYKNKIRPIFVKNFAMQVIYSFPIIKRALHKIAGKKLYQTFGGNLR
                     FLGVGGSKLDGVVEKFLADAGFPYAIGYGLTETSPLVAGAIPGKTKWQSTGPALEGVE
                     IKIDSPNSKGVGEIHVKGPNVMIGYFKDPAATANSFTNDGFFKTKDLGTIDKDGYLYI
                     KGRIDNMIVNSNGENIYPEEIETTLNEHDFVLESLVTQKNGKLVAIVYLNYEQIESMH
                     QFHDGVNVNLDERVRKVKEEILTYINGRVSKSSRISEIIEQPVPFEKTATQKIKRFLY
                     T"
     gene            complement(1010..3142)
                     /locus_tag="P5293_06650"
     CDS             complement(1010..3142)
                     /locus_tag="P5293_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR49614.1"
                     /translation="MNKQNWEFLNKFAINLNEEARAGKLDPVIGRDEEIRRVLQILSR
                     RTKNNPILVGEPGVGKTAIAEGIAQRIVHGDVPENLKTKEIFSLDMGALIAGAKYKGE
                     FEERLKGVVKEVIDSEGEVILFIDEIHTLIGAGRSDGAMDAANILKPALARGELRAIG
                     STTLDEYQLYFEKDKALERRFQMVMIDEPTTSETVSILRGLKERYENHHKVQIKDDAL
                     IAAVDLSHRYITNRHLPDKAIDLIDEAASKLRLEMNSVPEVIDELNHKIRQLEIEREA
                     IKREGDKEKLEDLTRQINELSAERDKNNEQWQKEKKILNEIQKQKMEIEQLKLMAEEA
                     ERSGDYGKVAELRYGKIVAAEKTIKELMEQKSKSEFTLINEYVEPEDIAEVVAKWTGI
                     PVKRMLESEKEKLLRMEDELHKRIVGQNEAIKAVSDAIRRSRAGLQDAKRPIGSFLFL
                     GTTGVGKTELAKALAEFLFNDENMMTRIDMSEYQERHSVSRLVGAPPGYIGYEEGGQL
                     TEAVRRKPYSVILLDEIEKAHPDVFNILLQVLDDGRLTDNKGRTADFKNTILIMTSNV
                     ASDVIQENLSKMNNVNRNEILEKTRNEVIEVLKATVRPEFLNRIDEIIMFHPLTMENV
                     KDILKMQLKNIQKLLEEKGISLIITDYAIEYLCNKGFDPAYGARPIKRLLQKELVNEL
                     AKTLLEGKLNKEKPIIVDCFDDVLVFRN"
     gene            3247..3870
                     /locus_tag="P5293_06655"
     CDS             3247..3870
                     /locus_tag="P5293_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRR49615.1"
                     /translation="MKFPELFDGALLPLAEAFYTIQGEGFHSGKPAFFIRLGGCDVGC
                     SWCDAKETWNPALYEPTPIEKIVSEAMKHPSRSIVITGGEPLSYPLDKLCNLLKINNF
                     DIFLETSGSKSMSGRFDWICLSPKKKRPPLKGMHQLASELKVIIETAEDFQWAENNRA
                     LVRKDCMLFLQPEWSRSKTILPIVIEYVKMNPSWNISLQIHKYMNIP"
     gene            3920..5113
                     /locus_tag="P5293_06660"
     CDS             3920..5113
                     /locus_tag="P5293_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRR49616.1"
                     /translation="MNKLFLIFASLIFLLTPAFSQEKPQNQTAYQFTNIKELPVTPVK
                     NQAKSGTCWSFSTMAFLEAELLKNGKGEYDLSEMFVVSNSYFDKADRFMRSYGKISFS
                     GGSSFSDALTIIRNNGIVPESVMNGLNYGEQLHNHGELDAVTAAYVNAISKNPNGKLS
                     TAWKNGFKGILDAYLGKIPDSFTFNGKEYSPQSFSAMLGLNYNDYVSITSYTHHPFYT
                     QFSLEVPDNWRNDLSYNIPIEEFMSIIDNAIEKGYPVLWGADVSEKGFTRKGIALVPE
                     QDVANMNGSDQQKWIGKTPQEIESKLNTLDEILPERKISQEDRQTAYDNLQTTDDHGM
                     LICGTAKDQNGTKYYIVKNSWGTSSGFKGFWYASETFVKYKTMNIVVNKNSIPKELRK
                     KMGID"
     gene            5125..>5611
                     /locus_tag="P5293_06665"
     CDS             5125..>5611
                     /locus_tag="P5293_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRR49617.1"
                     /translation="MKKRIFVITIISLFILSNLAFGQGYQFTDVCRNKATSVKNQART
                     GTCWCFATTAFIESELLRTGKGEFDLSEMFVVYQNYLNRLQDNYLRRGKGNIGEGSIA
                     HMLINVYETNGIVPQEVYNGINYDSDKHDHTELGLFLEAVANSAIKIKKRSPEYYEII
                     NN"
BASE COUNT         1748 a         1144 c          938 g         1781 t
ORIGIN      
        1 ccttcctctt tcacatgctt atgaatgcag cataggtatg atacaacctt tcactcatgg
       61 agctcatgta gtttatctcg atggttcacc aactccttct ttgcttttac ctgccattaa
      121 tgatgtcagg cccacaatta tgttgactgt acctcttatt atagaaaaaa tctacaaaaa
      181 caaaatccgc cccattttcg tcaaaaattt cgcgatgcag gtcatatact catttccaat
      241 aatcaaaaga gctcttcaca aaattgcagg gaaaaaactt taccaaactt ttggaggtaa
      301 cctgcgtttt cttggagttg gcgggtctaa acttgatgga gttgttgaaa aattcctggc
      361 tgatgccggt ttcccttacg ccatagggta tggtttgaca gagacttcgc cccttgttgc
      421 aggagccatc cccggcaaaa caaaatggca gtctacaggt cctgctctcg aaggagttga
      481 aattaaaata gactcaccaa actccaaagg agttggagag attcatgtaa aagggcccaa
      541 tgttatgata ggctatttca aagatcctgc tgcaactgca aatagcttta ctaatgacgg
      601 tttcttcaaa acaaaagact tgggaactat agacaaagat ggatatctat acattaaagg
      661 caggattgac aacatgatag ttaactctaa cggagaaaat atttatcctg aagagataga
      721 aacaacactc aacgaacacg attttgttct tgaatcatta gtgactcaaa aaaatgggaa
      781 attagtcgca attgtttatc ttaattatga acagattgaa tcaatgcatc aatttcatga
      841 tggtgtgaat gtcaatctag acgaaagagt tagaaaagtt aaggaggaaa tactcaccta
      901 cataaatggc agagtaagta aatcctcccg tatttcagaa attattgagc aacccgtacc
      961 ttttgaaaaa actgctaccc aaaagataaa acgcttcctt tatacataat tagtttctga
     1021 agaccagtac atcatcaaaa cagtctacta taatcggctt ttctttattg agtttaccct
     1081 caagtaaagt ttttgccaat tcattaacca actccttttg caacagcctt tttataggtc
     1141 tggcgccata cgctgggtcg aagcccttgt tacacagata ttcaatagca taatcagtaa
     1201 ttattaaaga aatgcctttt tcttccagga gtttctgaat atttttgagc tgcattttaa
     1261 gaatatcttt cacgttttcc attgtaagag gatgaaacat aattatctca tctatcctgt
     1321 tgagaaattc tggtctgacg gttgccttta aaacctcaat tacctcattt ctggttttct
     1381 caaggatctc gttcctgttc acattattca tttttgaaag attttcctga ataacgtcag
     1441 aagcaacatt tgaagtcata atcaaaatag tgtttttaaa atcagctgtc ctccccttat
     1501 tatcagtcaa ccttccatca tccaaaactt gcaatagtat attaaagacg tccggatgtg
     1561 ccttttctat ctcatcgagc aatataacag agtatggttt tcttctgaca gcctcagtaa
     1621 gctggccacc ttcttcgtaa ccaatatatc ctggaggagc gcctacaagt ctcgacactg
     1681 aatgcctttc ctgatattcg ctcatatcaa tacgggtcat catattctcg tcattaaaca
     1741 aaaattcggc aagtgctttg gcaagttctg tcttaccaac acctgtcgtt cctagaaaaa
     1801 gaaacgatcc aataggacgt tttgcatcct gcaagccagc cctgcttctt cttatagcat
     1861 cagacacagc tttaatagct tcgttctgac caacaattct tttatgaagc tcatcttcca
     1921 ttcttaaaag cttttctttt tcactctcaa gcattctttt aactggtata cctgtccatt
     1981 tagcaacaac ttctgcaata tcctcaggtt cgacatattc attgattaga gtaaattcac
     2041 ttttactctt ctgctccatg agctccttga ttgttttttc agcagcgact attttaccgt
     2101 atctcaattc agcaactttg ccataatcgc cacttctctc agcctcttct gccatcaact
     2161 taagctgttc tatctccatt ttctgtttct gaatctcatt aagtattttt ttctcctttt
     2221 gccattgttc gttattcttg tccctttctg cagacaattc atttatttgt cttgtcagat
     2281 cctcaagctt ttctttatct ccttcacgct taatagcttc tctttcaatc tcaagctgcc
     2341 ttatcttatg attgagttca tcaatcacct caggcacaga attcatttca agccttaatt
     2401 ttgatgctgc ctcatcaata agatcaatcg ctttatccgg taaatgtcta ttagttatgt
     2461 atcgatgaga aagatccacc gctgcaatca aagcatcatc cttaatttgc actttatgat
     2521 ggttttcata cctctcctta agccccctta aaatagagac agtttctgaa gtggttggtt
     2581 catctatcat aaccatttga aatcttcgct ctaatgcttt atctttttca aaataaagct
     2641 gatactcatc gagagtggtt gaacctatag ccctcaattc tcctcttgcc aaagcaggtt
     2701 taagaatatt agcagcgtcc atcgctccat cacttctccc ggcacctatc aaagtgtgaa
     2761 tttcatcaat aaaaagaata acttcgccct cactgtcgat gacctcctta acgacacctt
     2821 tcagtctctc ttcaaattca cctttgtact ttgcacctgc aattaatgct cccatatcaa
     2881 gagaaaaaat ctcttttgtt ttaagatttt ccggcacatc tccgtgtact attctctgag
     2941 cgataccttc cgcaattgct gtcttaccaa ctcctggttc accaaccaaa ataggattgt
     3001 ttttagttct acgacttaat atttgcagga ctcttcttat ctcttcatcc ctgccaatta
     3061 cagggtccag ctttccagcc cttgcttctt cattaagatt tatggcaaac ttgttaagaa
     3121 attcccaatt ttgcttattc ataattattt gtgtttttaa tggaaaatac tgcaacatat
     3181 ttgccatttc aatttttgct gtcaaaacgg catgttttac tacctttgta aaaaaaacat
     3241 acttacatga aatttccgga attgtttgat ggagcacttc ttccacttgc agaagctttt
     3301 tatacaatcc aaggagaagg ttttcacagt ggcaaacctg catttttcat acgacttgga
     3361 ggatgtgatg ttggttgtag ctggtgtgat gccaaagaaa cctggaatcc agccctttat
     3421 gaacctactc ccatagaaaa gattgtttct gaagccatga aacatccctc aagatcgatt
     3481 gttatcacag gaggagagcc gctttcatac cctcttgaca aattatgcaa tcttctaaaa
     3541 ataaacaatt ttgacatttt tctagaaact tcaggctcga aatcgatgag tggcagattt
     3601 gactggatat gcttgtctcc caagaaaaag agacctccac tgaaagggat gcaccagctg
     3661 gcttcggaac ttaaagttat tatagaaaca gccgaagact ttcaatgggc tgaaaacaac
     3721 agagcccttg tcagaaagga ttgcatgctt tttcttcagc ctgagtggag cagatcaaag
     3781 acaatactgc caatagtaat tgaatatgtc aaaatgaatc catcttggaa tatatcattg
     3841 caaattcaca aatacatgaa tattccttaa taaggttgga gttttttaat aactttgaaa
     3901 aaacttaatt tataaagtta tgaataaact ctttttgatt tttgcctctt taatttttct
     3961 tcttactcct gcattttctc aggagaaacc ccaaaatcaa acagcctacc agtttacgaa
     4021 tattaaggag ttacctgtaa cgcctgttaa aaatcaagcc aaatcgggca cttgctggag
     4081 tttctccact atggccttcc ttgaggcaga acttctcaaa aacggtaaag gtgaatatga
     4141 tctctctgaa atgtttgttg tatcaaattc ttattttgat aaggctgaca gatttatgcg
     4201 ttcttatggg aaaatcagtt tttcaggtgg aagctcattt tctgatgctc ttacaattat
     4261 cagaaacaat ggaattgtac cggaatctgt gatgaatggc cttaattatg gagaacaact
     4321 tcacaatcat ggagaattgg atgccgtcac tgctgcatac gtaaatgcaa tatctaaaaa
     4381 tcctaacggc aaactttcaa ccgcatggaa aaatggattt aaaggcatac ttgatgccta
     4441 tctcggaaaa attcctgata gtttcacatt taacggtaaa gagtattctc ctcaaagttt
     4501 ctcggcaatg ctggggctaa attacaatga ttatgtttca ataacttctt atacacacca
     4561 ccctttttat actcaatttt cgctggaagt cccagacaat tggagaaatg acctttcata
     4621 caatattccg attgaagaat ttatgtcaat cattgataat gcaattgaaa agggttatcc
     4681 tgttctttgg ggagccgatg taagtgaaaa gggatttacc agaaagggta ttgcactagt
     4741 tcctgaacag gacgttgcaa atatgaatgg atcagaccag caaaaatgga ttggaaaaac
     4801 tccgcaagaa attgagagta aattgaatac actcgacgaa atacttcctg aaaggaaaat
     4861 atcacaggaa gacagacaga cagcatacga caatcttcaa acaaccgatg atcatggaat
     4921 gctaatttgt ggaacagcaa aggatcagaa cggtaccaaa tattacattg tcaaaaactc
     4981 atggggaaca tcttcaggct ttaaaggatt ctggtacgcc tcagaaacat ttgtaaaata
     5041 taaaaccatg aatatagtgg ttaataaaaa ctctattcct aaagagttaa gaaaaaaaat
     5101 gggaattgac taatatcttt ggaaatgaaa aagcgaatat ttgttattac aattatttcc
     5161 ctgtttattt tatcaaatct tgcctttgga caggggtatc aatttacaga tgtatgtcgc
     5221 aacaaagcca catctgttaa aaatcaggcg agaacaggca catgttggtg ttttgctaca
     5281 accgccttta ttgaaagtga acttttaaga actggtaaag gagaatttga cctctctgaa
     5341 atgtttgtcg tatatcaaaa ctatcttaac agacttcagg ataattatct aagaagaggc
     5401 aaaggaaata ttggagaagg tagtattgca catatgctga ttaacgtata cgaaacaaac
     5461 ggaattgttc cacaagaggt atacaatgga attaactatg attccgacaa acatgatcac
     5521 acagaattgg gattgtttct tgaagctgtg gccaattcag ctataaaaat aaagaaaaga
     5581 agtccagaat attatgagat tattaataat a
//
LOCUS       DAOTQL010000109         5574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11727_length_5574_cov_5.442109, whole genome shotgun sequence.
ACCESSION   DAOTQL010000109 DAOTQL010000000
VERSION     DAOTQL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11727_length_5574_cov_5.442109"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..2157
                     /locus_tag="P5293_06670"
     CDS             79..2157
                     /locus_tag="P5293_06670"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR49618.1"
                     /translation="MKHFSIFLFFSSQNTPSGKDRVLLPFFNDYISEINVILGIVILM
                     GIALIVFAYFNFKITKSSKEYHRLSKENSERKKLLFNTLSAMSEGVITFDSQLNIIEI
                     NSAAKEMSSYSGDFIGHQFDEIFQTSQPESEIPVKEALQKAFKDKIRVTIANETKINY
                     LDRESRRIAGTISPVIDSEGNVAQLVFVFYDKTETFNQKRFLRLALESAKSFTWFFDT
                     FSNKLSLDENFSKYLGHNCNEEPDLSLLASKIHPDDRETLLKIHRKVGKESDKNFFQA
                     EYRLSFDGGKTYEWWESRGITFVDRSGNSDIKYIYGMDINIDEHKKRKQELLEAKIKA
                     EESDKLKTAFLSNMSHEIRTPLNGIVGFANLLTEPNYSDEEKKEFIEIINESSRRLLT
                     LIGDVLDLSKIESNTMTFDIRSFDLSDNINDVVNSYRNHDKPNVKVVTDYPKESVIVR
                     FDQVRNIQILNNLINNALKFTESGEVRVGYRVVENHVEVFVADTGIGIEENKIPFIFD
                     RFFKVNEYVSGTGIGLPLCKAIVTKLGGTIWAESQFGKGTTIRYTINLENTGDKSVKP
                     DSKKSVEKEKPLILIAEDMESNYQLLDVILCKQYKLLWAKNGEECLEMFKVHNPPLIL
                     MDIKMPVMDGLEATKIIRQYSKEVVIIAQTANAFESDHKIAIDAGCNDVIVKPIRASS
                     LLSTVKKYLS"
     gene            2177..4135
                     /locus_tag="P5293_06675"
     CDS             2177..4135
                     /locus_tag="P5293_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HRR49619.1"
                     /translation="MKNLKYLKLLSKSFPTIADAATEIINLEAILNLPKGTEHFLTDI
                     HGEHQAFQHVIKNASGVIKKKVEDIFGHTLREWEKKELCTLIYYPEEKLKIIKSREKE
                     IEDWYKMILVRLLKVCENVSSKYTRSKVRKALPKEFSYIIQELLHESLNNPNKHGYVE
                     AIISTIVSTGRAESFIIEISKLIQRMTIDSLHILGDVYDRGPGAHIIMDILCDYHNVD
                     IQWGNHDLIWMGAAAGSEACMANVIRICLRYTNLATLEDGYGINLLPLATFALETYGE
                     DPCSVFKPKMSEDEVVKQKQIKMISQMHKAISIIQFKLEGQVIERNPEMGMEDRRLLH
                     LIDYDKGTIMLRGKEYQLKDKNFPTIDPKNPYKLTEDEKELVDKLMHSFTHSEKLRKH
                     IRFIYSKGSLYLVRNSNLLYHGSVPMNSDGTFKNVRIQGIDYSGKQLFDKIDQVVRQA
                     YFEEKKAKEKRFGQDFIWYLWCGPSSPPFDKDKMATFERYFIADKETHKEQQGHYFYL
                     KDKKEICEMIIKEFGVEDEHARIINGHIPVKTIKGESPIKAGGKLLVIDGGYSKAYQS
                     ETGIAGFTLIYNSHGLQLVQHQPFVSTQQAIEKGEDIISETTVLEFSNQRKLVRDIDI
                     GKELMQQIEDLTHLLDAYRSGYLKELDN"
     gene            complement(4146..5048)
                     /locus_tag="P5293_06680"
     CDS             complement(4146..5048)
                     /locus_tag="P5293_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRR49620.1"
                     /translation="MQTTSKVVMVRPANFGYNPETAANNAFQKRGFEDEAHLKALNEF
                     DNYVKLLRENGVFVTVIQDTPEPCTPDSIFPNNWFSTHEGGTLVLYSMYAGNRRLERK
                     PEVIAAIKSTFDVKRIVDLTKWESDSLFLEGTGSMVLDRDNDLVYACLSPRTAKPVLE
                     EFCDELNYDYFLFHAFDLNGQPIYHTNVMMCVGNKFVVACLDAIRDIGEREDFIALVE
                     ETEKELIEISLDQLNNFAGNMLELKSCNGDPLLLMSQRAKLSLNEEQIAKLSSYCKII
                     APDLATIENNGGGSARCMIAEIFL"
     gene            complement(5062..>5574)
                     /locus_tag="P5293_06685"
     CDS             complement(5062..>5574)
                     /locus_tag="P5293_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR49621.1"
                     /translation="RTGKMLCCDHENVRPDIVILGKAISGGVLPVSAVLADDDIMLTI
                     KPGEHGSTYGGFPLACRVAVAALEVVKEEKLAEKAEYLGKIFREELGKCKSPFIKQIR
                     GKGLLNAVVIEPQNGKEAWDVCERMAENGLLAKPTHRHIIRFAPPLVITEAELRDAIK
                     IIVSSLESLL"
BASE COUNT         1925 a         1082 c         1026 g         1541 t
ORIGIN      
        1 atggcaaaaa aagaagtttt gggcaaggtt ctttaaaaag ttttaataac tttgataaag
       61 atctgagtaa aaatcagcat gaaacacttc tcaatctttc tttttttctc gtcgcaaaat
      121 acaccttctg gcaaagacag agtgctactg ccatttttca acgattatat ttcggagata
      181 aatgtcattt tgggtatagt tattttaatg ggtattgcat taatcgtctt tgcctacttt
      241 aattttaaaa taactaagtc ttcaaaggaa taccacagac tttccaaaga aaattctgaa
      301 aggaaaaagc ttctcttcaa tactctgtca gctatgagcg agggagtcat aacttttgat
      361 agtcaattaa atattattga gattaatagt gctgctaaag agatgtcaag ttacagtggc
      421 gattttatag gacaccaatt tgacgaaata tttcaaactt ctcagccaga aagtgaaatt
      481 cccgttaagg aagctcttca aaaagcattt aaggataaaa tccgagtcac aattgcaaat
      541 gaaacaaaga taaactacct tgacagagaa tcaagaagga ttgccggaac aatctcgcca
      601 gttatagatt ccgaaggcaa tgttgcccaa ctcgtgtttg ttttttacga taaaactgaa
      661 actttcaatc aaaaacggtt tcttagactt gccttggaat cagcaaaatc ttttacctgg
      721 ttttttgata cattcagcaa taaactttct cttgatgaaa acttttctaa atacctcgga
      781 cataactgta atgaagagcc agacttatct cttttagcat caaaaattca ccctgatgat
      841 agggaaacct tattaaaaat acacagaaag gttggcaaag agtctgacaa gaattttttt
      901 caagcagagt acagattatc ctttgatggg ggaaaaacat atgagtggtg ggagagcaga
      961 ggcattacct ttgtcgacag aagcgggaac agtgacataa agtatattta cgggatggac
     1021 atcaacattg atgaacacaa aaaaagaaag caagaactac ttgaggcaaa aattaaggct
     1081 gaggagtcgg ataagctcaa aacagctttc ctctcgaaca tgagtcacga aatacggaca
     1141 cctttgaatg gcattgttgg tttcgctaat ttactgacag aacctaatta cagtgatgaa
     1201 gagaaaaaag agtttataga gatcatcaat gaaagctcaa gaaggttgct tactctcatt
     1261 ggcgatgtac ttgacctttc taagattgaa tccaacacta tgacatttga catcagatct
     1321 ttcgatctgt ctgacaacat taatgatgtt gtcaattcct atagaaatca tgacaaacca
     1381 aatgtgaaag tcgtgacaga ctatccaaaa gaatcagtga ttgttaggtt cgatcaagtt
     1441 cgaaatatcc aaatcctcaa caacctaatt aataatgctc tcaaatttac cgagagtggt
     1501 gaagtgagag ttggttacag agttgtagaa aatcatgttg aagtttttgt agctgacacg
     1561 ggaattggga ttgaggagaa caaaatacct ttcatttttg acagattttt taaagtaaac
     1621 gaatatgtga gcggaacagg cataggtctt ccactttgca aagcaattgt cacaaaactt
     1681 ggcggaacaa tctgggctga atcacagttc ggaaaaggca caacaataag atataccatc
     1741 aatttagaaa atactggaga caagtctgtt aagccagact caaaaaaatc agtcgaaaaa
     1801 gaaaagccac taattcttat tgctgaagat atggaaagta attaccaact gcttgatgtg
     1861 atcctttgca aacaatataa acttctttgg gcaaaaaatg gagaagagtg tttagaaatg
     1921 ttcaaggtgc ataatcctcc acttatactc atggatataa aaatgcctgt catggatggt
     1981 cttgaagcca ccaaaattat tcgccaatat tcaaaagagg tagtaattat agcccaaaca
     2041 gcaaacgcct ttgaatcaga tcacaaaatt gccattgatg cagggtgcaa tgatgttatt
     2101 gtcaaaccta ttcgtgcatc atctctgctt tccactgtaa agaaatactt atcataaaca
     2161 acaatatttt acaatcatga agaaccttaa gtacctgaaa ttgctctcaa agagcttccc
     2221 aaccattgcg gatgctgcga ccgagattat caatcttgag gcaattctca acctaccaaa
     2281 aggaacggaa cattttttaa cagacattca cggagagcat caagcttttc aacatgtaat
     2341 taagaatgcc tcaggtgtta ttaagaagaa agttgaggat atcttcggac acactctcag
     2401 agaatgggag aaaaaagaat tatgcacact gatctactat cctgaagaaa aactcaaaat
     2461 tatcaaatca agggaaaagg aaatagaaga ttggtataag atgatattgg tcagactctt
     2521 gaaagtctgc gaaaatgtct cctcaaaata cacgagatca aaagtcagga aagcgctgcc
     2581 aaaagagttt tcatacataa tacaggagct tcttcatgaa tccctgaaca acccaaacaa
     2641 acacggctat gttgaagcca ttatttcaac aattgtgtct acaggcagag cagagagttt
     2701 tataatagag ataagtaaac ttatacaaag gatgaccatt gattctctgc acattctggg
     2761 agatgtatat gacagaggac ctggagcaca cataatcatg gacattttgt gtgactatca
     2821 caatgtcgac attcaatggg gcaatcatga ccttatctgg atgggagcag cagcaggaag
     2881 cgaagcctgc atggccaacg taattcgaat ttgtttacga tataccaatc tggcaacact
     2941 tgaagatgga tatggcataa atctccttcc tctggcaaca tttgccctcg aaacatatgg
     3001 agaagatcct tgctccgttt tcaaaccaaa aatgagcgag gatgaagtgg tcaagcagaa
     3061 acagataaaa atgatatcac agatgcataa agctatctca attatacaat ttaaacttga
     3121 agggcaagta atcgaaagga accctgaaat gggaatggaa gatcgaagac ttcttcattt
     3181 gattgactac gacaaaggta caataatgct tagaggcaaa gaatatcagc ttaaggataa
     3241 aaatttccct accattgatc cgaaaaaccc atataaactt actgaagatg aaaaagagct
     3301 ggttgacaaa ctgatgcact catttactca tagcgaaaaa cttcgtaaac atatccgatt
     3361 catttattca aaaggttctc tttatttagt aagaaattct aatttgcttt atcacggatc
     3421 tgttccaatg aactcagacg ggacatttaa aaatgtccga attcaaggca ttgattacag
     3481 cggcaagcaa cttttcgaca aaatagatca ggtagtacgt caagcctatt ttgaggagaa
     3541 aaaagctaaa gagaagagat ttgggcaaga ctttatctgg tatctctggt gtggcccttc
     3601 ttctcctcca ttcgacaagg acaaaatggc cacgtttgaa aggtatttca ttgctgacaa
     3661 ggagacacat aaagaacaac aaggccacta tttttattta aaggacaaga aagagatatg
     3721 tgagatgatt ataaaagagt ttggagttga agacgaacat gccagaataa ttaatggaca
     3781 cattcccgtg aagacaatca agggggaatc tcccataaaa gctggaggaa aattgcttgt
     3841 tattgatggt ggttattcaa aggcttatca gtcagaaaca ggaattgcag gattcactct
     3901 tatctacaat tcacatggac tgcaactggt gcaacatcaa ccgtttgtat ccacacaaca
     3961 agctattgag aaaggtgaag atattatctc cgaaacaacc gttctcgagt tcagtaatca
     4021 acgaaaactt gttagagata tagacatagg caaagagctt atgcaacaga tagaagacct
     4081 cactcatctc cttgatgcat atagaagtgg ctatctcaag gagttagata attgatcaaa
     4141 actaactaaa gaaaaatctc cgcaatcatg caccgggctg aaccaccacc attattttca
     4201 atagtggcaa ggtccggtgc aattatttta cagtaggaag agagtttagc aatctgctcc
     4261 tcattcagcg aaagtttcgc tctctgcgac ataagtaata gtggatcacc attacagctt
     4321 tttaactcga gcatatttcc tgcaaaatta ttcagctgat caagtgatat ctctatcaat
     4381 tccttttctg tttcctcgac aagcgcaata aaatcttcac gttcaccaat atctcttatt
     4441 gcatccagac atgcaaccac aaatttgttc cctacacaca tcataacatt tgtatgatag
     4501 attggctgac cattcaaatc aaaagcatgg aaaagaaaat aatcataatt taactcatca
     4561 cagaactcct ccagtacagg cttggctgtt ctaggagaaa gacaagcata tacaagatca
     4621 ttgtcccggt caagcaccat acttcctgta ccttcaagaa acagagagtc tgactcccac
     4681 ttagtcaaat ctacgattct cttaacgtca aacgtactct taatggcagc aattacctca
     4741 ggtttcctct caagtctcct attaccggca tacatggaat aaagcacaag tgtaccccct
     4801 tcatgagtag aaaaccagtt gttcggaaaa atcgagtcag gagtacatgg ttccggagta
     4861 tcctgtatta cagtaacaaa tactccattc tctcttagaa gtttgacata attatcaaac
     4921 tcgttcaaag ctttcaaatg ggcttcatct tcaaatcctc ttttttgaaa ggcattgtta
     4981 gctgctgtct caggattgta tccaaagttt gcaggtctta ccatcacaac cttagaagtt
     5041 gtctgcatat taaataaatg attataatag tgactcgaga gagctgacaa taatttttat
     5101 agcatccctc aattctgctt cagttattac aagaggagga gcaaaacgaa taatatgcct
     5161 gtgggttggc ttagcgagaa gaccattctc agccatcctt tcacaaacat cccaagcttc
     5221 tttaccgttt tgaggctcaa taactactgc attgagtaac cctttgcctc ttatctgttt
     5281 gataaatgga gatttacatt tgcccaattc ctctctgaaa attttaccta aatattcagc
     5341 cttctccgcc agtttctctt cctttactac ctcaagagca gctaccgcaa cccggcaagc
     5401 caaaggaaag ccgccatatg tagaaccatg ttctccaggc ttaatggtca acattatgtc
     5461 atcatctgca agtacggctg aaaccggcaa tactcctccc gaaatagctt tacccagaat
     5521 aacgatatcc ggcctcacat tctcatggtc acaacataac atttttcccg tacg
//
LOCUS       DAOTQL010000110         5572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11732_length_5572_cov_5.270618, whole genome shotgun sequence.
ACCESSION   DAOTQL010000110 DAOTQL010000000
VERSION     DAOTQL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11732_length_5572_cov_5.270618"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="P5293_06690"
     CDS             <1..1308
                     /locus_tag="P5293_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRR49622.1"
                     /translation="ERSRNATSTEESDWIELRFFNGVEFVGYDKVEEQISIVKYRKIK
                     AKNKEYYQLVFDKTPFYAESGGQVGDTGYIESADGIQYSITNTVKENNLIVHIADTLP
                     ARTDLPFTAKVDLKKRFSTANNHTATHLLHRALRELLGNHIEQKGSLVNFNYFRFDFS
                     HYEKISDEMIRKIENRVNELIRENSPREEFREVPIEEARKIGAMALFGEKYGDRVRVI
                     RFGNSIELCGGTHTLATGNIGYFKILSESAIAAGVRRIEAVTGEYAERVVEEFEDTLK
                     SIRSILNNNPSLVTAIQKLVSENEMLHKTIEEELKEKVKKLKESLIEKAREINGMKIV
                     TMSGAYPAEVVKKIASMIHNEMNNTVFIAGCESEGKATLTLMYTDDLTTKGYNAAKDI
                     REAAPLISGGGGGQNFLASAGGKNITGLAAAIVKLTELATQQR"
     gene            1305..2750
                     /locus_tag="P5293_06695"
     CDS             1305..2750
                     /locus_tag="P5293_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRR49623.1"
                     /translation="MKKIDQFILKSFLGPFILTFLIVTFALLLQFLWLYIDELVGKGL
                     GFGVIAEFLGWGAATLIPLALPLATLFASIMTMGGFGENSELLALKAAGISLQRILAP
                     LIVLSFIISIGAFFASNNLIPVAYNKIYALRDDIGKTKEEIRIPTGMFYDGIDGYVIR
                     INQRDKKTGNIYGVMVYDHTSQTGNNLTVADSGSIKLTADKANLIFTLYSGYSYQEPF
                     HFRGDTSYTMQKIGFDKQDLIISLKDYAFKRSDASRFSKEIMSQNIKQLSYLKDSLDS
                     AYTIIKSRQLQDLIMAGGFSVPTQFDLSKTKKFKGEFNADSLLKFKNLYDEQIVLEGA
                     LSASLEATTILDNYQFDEYRYSHPLRKVQVERFRKFTLSFACFIFFFIGAPLGAIIRK
                     GGLGTPVVISMFFFIIYWVIDISGKKLANDGVISPAIGTFISTFVLLPMGAYLTWKST
                     QDSSLFNAEIYKNLFKRIIRLNFKKENYGET"
     gene            2737..3708
                     /gene="fmt"
                     /locus_tag="P5293_06700"
     CDS             2737..3708
                     /gene="fmt"
                     /locus_tag="P5293_06700"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRR49624.1"
                     /translation="MEKRDVRIIFMGTPTFAVTQLEALLEENYNVVAVVTAPDKPAGR
                     GMQLVSSEVKKFAISRSIPVLQPTSLKNESFLAELSSYKADLFVVVAFRMLPKEVWAM
                     PRLGTFNLHASLLPDYRGAAPINHVIINGETVTGVTTFMLDEKIDNGAILHRQECSIS
                     PEDNFGTLHDKLLHIGKELIIKSIEEIISGTIKPVPQSLIVKDIATLHTAPKITKEMC
                     KIDWNMAPEKIHNLVRGLSPYPGAYSTLSGKGKEIPVKIFKTEFVNENHRLPMGKILT
                     DKKEEFKVACKEGFIKIMELQVAGKRKMSVKDFLAGFRDLNLYMFIQ"
     gene            3705..4325
                     /locus_tag="P5293_06705"
     CDS             3705..4325
                     /locus_tag="P5293_06705"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRR49625.1"
                     /translation="MKTFVILADGEFPKRQQLLEILVSSSGIICCDGAAAALVEKGYT
                     PIAIVGDMDSLDKNFQEKYRGIIYHNPDQETNDLTKAFIYTLSLSPDQIFILGATGLR
                     EDHTIGNVSLLALYSDMTDVPVRILTDYGIFTCIEGDTVIKTSIGTQISIFALDKETK
                     IVSEGLKYPLKDVILDSWWKGTLNEATLDSVKLNIDKGKVIVFQAY"
     gene            4351..5424
                     /locus_tag="P5293_06710"
     CDS             4351..5424
                     /locus_tag="P5293_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRR49626.1"
                     /translation="MKFGLIKKVTTVTILSGLIITLNPADAISQTVDEYIKEDVHRHD
                     EVIKKIDDVLWYQKVGDIAYVDKVRLTGPPRWKPRTEGDRFAGNPLQFYAYTFIPKNV
                     KKNHKYPLIVLPHSGIHADFSTYYAHIVRELISQGYIVVAAEYRGSTGYGKGVYENID
                     YGGLENEDVLASRDYMTTNYSIVDSNRVAIIGWSHGGMISLFNILKYPEKYVCAFAGV
                     PVSDLNSRLTSHGKSYEDYFTPKYHIGKSLKEDPEEYKRRSPSSYAALLQRPLLIHTN
                     TIDNDVYVEEVMCMIDSLKAHDKKFEYKVFNAAAGGHGFDRIDTKEATDIRFTIYKFL
                     ERYLNPPKPFKSPDEMRKAAYHF"
BASE COUNT         1875 a         1000 c         1111 g         1586 t
ORIGIN      
        1 gagagaagtc gtaatgcgac ctccacagaa gaaagtgact ggatagaact tcgttttttt
       61 aatggggtcg aatttgtggg gtacgacaaa gttgaagagc aaataagcat cgttaaatac
      121 cgtaaaatca aggcaaaaaa taaagaatac tatcaacttg tttttgacaa aactcccttt
      181 tatgccgaaa gcggagggca ggttggagac acaggttata ttgaatctgc tgatggaata
      241 cagtattcta taactaatac cgttaaagaa aacaatctta tagtacatat tgcggacaca
      301 cttcctgcta ggactgactt accatttaca gcaaaagttg atcttaaaaa acgattttcc
      361 actgcaaata accatactgc tacacatttg ttgcacaggg cattgagaga gttacttgga
      421 aatcatattg aacaaaaggg gtcactcgtc aatttcaatt atttcagatt tgacttttct
      481 cactatgaaa agatcagtga tgaaatgatc cgaaaaatag aaaaccgcgt taacgaactg
      541 ataagagaga actccccaag agaagagttc agagaggttc ctattgaaga ggcacgtaaa
      601 ataggagcca tggcactttt tggagagaaa tacggggaca gggttagggt gattcgtttt
      661 ggcaattcta ttgaactgtg tggtggaaca catactttgg caacaggtaa tattgggtat
      721 ttcaaaattc tttcggaatc agcgattgct gcaggtgtaa gacgaatcga agctgttaca
      781 ggagaatacg cagagagagt tgtcgaggaa tttgaagata cactaaaatc tatacggagc
      841 atactcaaca ataatccaag tcttgtgaca gctattcaga aacttgtaag cgaaaacgag
      901 atgttgcata aaactattga agaggaatta aaagagaaag taaaaaaatt gaaagagtca
      961 ctaatcgaaa aagcaagaga gatcaatgga atgaaaattg taaccatgtc cggtgcttat
     1021 cctgctgagg ttgttaaaaa gattgcttcg atgatacata atgagatgaa caataccgta
     1081 tttattgccg gttgtgaatc agaagggaaa gctacactga ctctgatgta tactgacgat
     1141 ctgacaacca aaggatataa cgcagctaaa gacatacggg aagcggcgcc attgattagt
     1201 ggaggaggag gaggacagaa tttccttgca agcgcaggtg gcaaaaacat tacaggtctt
     1261 gctgctgcaa ttgttaaact aacagagctg gctacacaac agaggtgaaa aaaatagatc
     1321 aatttattct caagtcattt ttaggaccgt tcattttaac cttcctcatt gtcacttttg
     1381 cccttttgct gcagttcctc tggttataca tcgatgaact tgtaggaaag ggactcgggt
     1441 ttggtgttat tgcagaattt cttggatggg gagcagctac cctcataccc cttgcacttc
     1501 ctctcgcaac cctgtttgca tcaattatga ctatgggagg atttggagaa aacagtgaac
     1561 ttcttgcatt gaaagcggca ggaatctctc ttcaaaggat cctcgctcct cttattgtgc
     1621 tctcattcat aataagtatc ggcgcttttt ttgcttcaaa caaccttata cctgttgcct
     1681 ataataaaat atacgcacta agggatgata tcggaaaaac aaaggaagag atacgaattc
     1741 ctacaggtat gttttatgac ggtatagacg gatatgttat tcgaatcaat caaagagaca
     1801 agaaaaccgg taacatttat ggagttatgg tgtatgacca tacttctcaa acaggcaaca
     1861 atctcaccgt tgcagattca ggttctataa agctcacagc tgacaaggca aacctaatat
     1921 ttacactcta tagtggatat tcctatcaag aaccttttca ctttagagga gacacttctt
     1981 atacaatgca gaaaattggc ttcgacaaac aggatcttat tatttctctc aaggactatg
     2041 ctttcaaaag gtctgatgcc agcagattca gtaaagaaat tatgtcgcaa aatatcaaac
     2101 aattatctta cttgaaggat tctctcgact ctgcttatac aattattaag agtagacaac
     2161 ttcaggatct tattatggct ggaggatttt cagttcccac acaatttgat ctttcgaaga
     2221 caaaaaagtt taaaggggag tttaatgccg attcattgct taaatttaaa aatctctatg
     2281 atgagcaaat agttcttgaa ggagctctct cagcctcgtt agaagcaact actattctcg
     2341 acaactatca gttcgatgaa tacagatact cacaccctct tagaaaagta caagttgaaa
     2401 gatttagaaa attcaccctc tcatttgcct gttttatatt tttctttatt ggggcaccgc
     2461 ttggagcaat tatacgcaaa gggggtttag gcactcctgt tgtaatatct atgttctttt
     2521 tcataatata ttgggtcata gatataagtg gcaaaaagct tgcaaacgat ggggttatct
     2581 ctccggcaat tggcactttt atctccacct ttgtattact accaatggga gcttatctaa
     2641 cttggaaatc gacacaggac tcctctctgt tcaacgcaga gatatacaaa aacctcttta
     2701 aaaggattat aagactcaac ttcaaaaaag agaactatgg agaaacgtga tgtcagaatt
     2761 atatttatgg gaactccaac ttttgcagtt acccaactcg aagctctact tgaagaaaat
     2821 tataatgtag tggcagtagt cacagccccc gataaaccgg ccggtagggg catgcaactt
     2881 gtttcaagtg aagttaagaa atttgcaatt agcaggtcta tccccgtact tcagccaaca
     2941 tcactgaaga atgagtcttt tcttgcagaa ctctcttcat ataaagcaga tctatttgtc
     3001 gttgttgcct tcagaatgtt gcctaaagaa gtatgggcaa tgccaagatt agggacattc
     3061 aatcttcatg catcattact tccagattat aggggagctg ctcctattaa ccatgttatc
     3121 atcaacggtg aaactgttac cggtgttaca acattcatgc ttgatgaaaa gattgataat
     3181 ggagctattt tgcatcgtca ggaatgttcc atctcacctg aggacaattt tggcactctt
     3241 cacgacaagt tgctgcatat tggcaaagag ttgataatca aaagtattga agagataata
     3301 agtggcacaa taaaaccagt cccccaatca ttgattgtca aagatatcgc aactcttcat
     3361 actgctccca agattacaaa agaaatgtgc aaaatagact ggaatatggc acccgagaaa
     3421 attcacaatc ttgtgagagg actcagccct tatcccggtg cctattctac gctttccggc
     3481 aaaggcaaag agatacctgt caaaattttc aaaacagagt ttgtaaatga gaatcacaga
     3541 ctgcctatgg ggaaaattct tactgataaa aaagaagagt ttaaagttgc ctgcaaagaa
     3601 ggatttatta aaataatgga acttcaggtt gcaggaaaaa ggaaaatgtc tgtaaaggat
     3661 tttttagctg gttttagaga cctgaatcta tatatgttta tccaatgaaa acttttgtaa
     3721 ttctggccga tggtgaattc ccaaaacgtc aacaattgtt ggaaatactt gtgagttcct
     3781 ccggaattat ctgctgcgat ggagcagctg ctgcacttgt cgagaaaggt tatactccaa
     3841 ttgcaattgt aggagacatg gatagccttg ataaaaattt tcaggaaaaa tatcgaggaa
     3901 taatatatca caatccggat caggagacca atgatcttac aaaagcattt atatatacac
     3961 tttcactgtc tcctgaccag atatttatac ttggtgccac aggtctaagg gaagatcata
     4021 ccattggtaa tgtctcttta cttgcacttt acagtgatat gacagatgtt cctgtaagaa
     4081 ttttaactga ctatggaatt ttcacatgta ttgaaggtga cactgttatt aaaacaagta
     4141 ttggcacaca aatttcaatt ttcgcccttg acaaagagac gaaaatagtt tcagaaggtt
     4201 tgaaatatcc tttaaaagat gttatattag acagttggtg gaaaggcaca ctcaatgaag
     4261 ctactttaga tagtgtcaag ttgaatattg ataaaggaaa agttatagtt ttccaagcat
     4321 attaagattt taaccaataa actcattact atgaaatttg gacttataaa aaaggtgaca
     4381 acggtaacaa ttctgtccgg cctcattata acattaaatc cggcagatgc catatcgcag
     4441 actgtagatg aatacattaa ggaagatgta cacaggcatg atgaggtaat taaaaaaatt
     4501 gatgacgttt tatggtatca aaaggtagga gacattgctt acgtagacaa ggtaagactt
     4561 acaggccctc caagatggaa accgagaaca gaaggagaca gatttgcagg taatcctttg
     4621 caattttatg cctatacttt cattcctaag aatgtgaaaa aaaatcacaa atacccactc
     4681 atagtccttc ctcatagcgg aattcatgca gacttttcta catattatgc acatatcgtg
     4741 agggaactta tcagccaggg ttacatagtt gtagctgcag aatacagagg aagtaccggt
     4801 tatggaaaag gtgtatatga aaacatcgat tatggtggat tggaaaatga agacgtactt
     4861 gcaagcagag actacatgac aacaaactac agtattgtgg acagcaacag agtggcaatt
     4921 atcggctgga gtcacggagg catgatatct cttttcaata ttctgaaata tccggagaaa
     4981 tatgtttgtg cctttgccgg ggtacctgta agcgacctta acagcagact aacctcccat
     5041 ggaaaaagtt atgaagatta tttcactcca aaatatcata tagggaaaag tctaaaagag
     5101 gatcctgagg aatataaacg tcgttcacca tcatcctatg cagctttgct tcaaagacct
     5161 ttgctgatac ataccaacac tattgacaac gatgtttatg ttgaagaagt tatgtgcatg
     5221 atcgattctc taaaggcaca tgacaaaaag tttgaataca aggttttcaa tgcggctgcc
     5281 ggaggtcacg gatttgacag gattgacact aaagaagcaa ctgatatacg ttttacaatt
     5341 tacaaatttc tcgaacgata tcttaatcct ccaaaacctt ttaaatctcc tgatgagatg
     5401 aggaaggctg catatcactt ttaaatttaa atacttttat tccacccaaa gaattgaatc
     5461 acaaatgcta tgtttaaatt tatccggtga aatttgctgc ttaaaatgga aattgaaaaa
     5521 taaaaaaaga ggtgtccaaa actttctgga cacctctttt taaaaagatg gc
//
LOCUS       DAOTQL010000111         5491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_11931_length_5491_cov_3.897351, whole genome shotgun sequence.
ACCESSION   DAOTQL010000111 DAOTQL010000000
VERSION     DAOTQL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11931_length_5491_cov_3.897351"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="P5293_06715"
     CDS             complement(<1..202)
                     /locus_tag="P5293_06715"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911264.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRR49627.1"
                     /translation="MAKEVSNKEENYALWYNNLVIKSELAENSAVRGCMVIKPYGYAI
                     WEKMQAQLDRMFKETGHQNAYFP"
     gene            301..1494
                     /locus_tag="P5293_06720"
     CDS             301..1494
                     /locus_tag="P5293_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49628.1"
                     /translation="MKNKLYYFAAVILALSSCGTESRYSSASYEDPAYYRPGTDIAVI
                     RKSDDQSATDKELQALKEKTKEVIVVDEKSGKTVLPLSKADTVLVDINEYDSYEELLT
                     KFNSPEYEIHFTVNDYSNWWSYPWAWNHPYYYWNPSWRFTFNWYYGYWDPWYYSSWSW
                     YNPYYYDYWWYRDLYYGWWGPRYDLYGYWPSGGWYSGPYWVVPVERHLYGRRDSDNSN
                     PNQRHSGINSSGSYTRMSKAEISQIRGSSVSNSGNRPSQSVYRRENSQDRFTTSSGKV
                     INQSSGNSNIVRNANSGNTGIKSSAHSTSPKKSEYTRSTGNYRVPVRQNNAASGSNSG
                     SGISTGKNSSTNRSEFQRSNINNTYSQPTRNSGTFQSSGSSTQSSQSSGSGRSSSGSG
                     SVYRR"
     gene            1501..3051
                     /locus_tag="P5293_06725"
     CDS             1501..3051
                     /locus_tag="P5293_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRR49629.1"
                     /translation="MKKTFTFTVAILLTTLLSAQNRYDALRYSQNFYEGSAKSLAMGN
                     AIVSIGGDLGAISINPASAGLYRYSEFMFTPSTISTLTETDYLDRNTDKRFFRAGVSS
                     FGFVTPLNGIRKTGNLKGLNVSVSMNKLNNFNNRVSAKGRADNTSWLSFLAYNTKGIN
                     SSELDIQSVGDTYPFYNSGASWSSVLAWNTNLLDRLPDSESKYIAATENIVGNSNVIG
                     GPLDQGFKRETTGGISEYAITLSGNVNNKFYFGFSLGLQSLRYTDWQSYSEFAVNLNQ
                     FDSKFKDFEHIYKQTTTGVGINARAGVIYVPVEGLRLGASLATPTYFSLEDEWRESMY
                     AEYSDNYHVFLKSPKGSYDYLVITPARANLGISYFFGTRGSISLDYEFVGYEWMRMDD
                     ADSDFDEFDNENDLIEEDFQNTSNIRAGAEFRLNRIFSLRAGYSFYQNPERNYGFDTQ
                     FVSCGAGFKFGSGFFADFGIQRRISNSENFRLYENVPLQGGSSLLAPTGESTYTAWKM
                     LATIGFRF"
     gene            complement(3048..3680)
                     /locus_tag="P5293_06730"
     CDS             complement(3048..3680)
                     /locus_tag="P5293_06730"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRR49630.1"
                     /translation="MDNSHSASVLLSTAYLPSIEYIRAIANSGRPVIERYENYQKQSY
                     RNRCYIYSSAGLLPLIIPICREDRNDEDTRRIDKVKIDYTKKWQKQHWRAIFSAYNSS
                     PFFCYYKDEIYSFYTEEEPLLFNYNNRLLNTILDLIGISSVPEFTQNYIRDFEGNDYR
                     NYFHPKKEVLFNNKYGQYYQVFAHKYGFIPNLSIIDLLFNEGPNSITFFF"
     gene            complement(3673..4461)
                     /gene="lpxA"
                     /locus_tag="P5293_06735"
     CDS             complement(3673..4461)
                     /gene="lpxA"
                     /locus_tag="P5293_06735"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRR49631.1"
                     /translation="MSNTFIHPKARIGNNVTVGPFSYIAENVEIGDGTIIGPHVTILD
                     YVKLGSNNKIYPGAVLGSTPQDVGFAEEETWLEIGDGNIIREFANMARGTIHGRGKTV
                     MGNNNMLMSYSHVAHDCVIGNGTVIISYVGFAGFVEVNDYATIGGKAAFHQFTRVGAY
                     SMVSGASAVRKDVPPYALTGNNPVAFYKLNIVGLRRNGFTSEQISRIESIYRTLYDSG
                     LNISDACERIEQDFEESTEKRTILDFIKNSKRGIVPKISRLIHG"
     gene            complement(4473..>5491)
                     /gene="fabZ"
                     /locus_tag="P5293_06740"
     CDS             complement(4473..>5491)
                     /gene="fabZ"
                     /locus_tag="P5293_06740"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRR49632.1"
                     /translation="APSNYYTPERTIYYKDDKSGSEITIEPASELMIDVTIDFDSDII
                     GKQRVIFDNNTDFLTEIAPCRTFVFIHDILPLLKQGLIKGGDIDNAIVIADKPLSQED
                     LDFIKNSYPGVLKGDLSRGYINESGLRFKDECARHKLVDLIGDLALTGKRIRARITAY
                     KPGHSINTIVAKIMRNDLMRSDAIPQYDPNKEPLVDINGIKKLLPHRPPFLMVDRIIE
                     MTENVVVGIKTIGINEGYFIGHFPDEPVMPGVLILESMAQVGGILVLNEVDEPEKYST
                     YFAKIDKVRFKRKVVPGDVIVIKMTLTQPIRRSIVYMKGEVYVGDSLVCEGEMVAQVI
                     KNKE"
BASE COUNT         1685 a         1046 c         1015 g         1745 t
ORIGIN      
        1 acggaaaata agcattctga tgacctgtct ccttaaacat tctgtcaagc tgagcctgca
       61 tcttttccca aatagcataa ccatagggct ttatgaccat acatcctctt actgctgagt
      121 tttcagccaa ttcagacttg ataaccaaat tattatacca taatgcataa ttctcctctt
      181 tgttgctaac ttcttttgcc attacctaaa taaaatattt gtcagtggta caatttttgt
      241 gtaaatttgt attgaacagc acaaaagtat aaataatatt taatacacag gaggtgtgtt
      301 atgaaaaaca aactttatta ctttgcagcc gttattcttg cattgagctc ttgcggcacc
      361 gaaagtcgtt attcatctgc ctcttacgaa gatcctgcat attatagacc tggtactgat
      421 atagcagtaa ttcgtaaatc ggatgaccaa tctgcgactg acaaggagct gcaggcactg
      481 aaggaaaaga cgaaggaagt aattgtagta gacgagaaaa gcggtaagac agtcctccca
      541 ttatccaaag ccgacactgt tcttgttgac atcaatgaat atgattcata tgaggaattg
      601 cttaccaaat ttaattcacc agaatatgaa atacatttca cagtaaatga ttattctaac
      661 tggtggagct atccatgggc atggaatcat ccatactatt attggaatcc gagttggaga
      721 tttaccttca attggtatta tgggtattgg gatccatggt attattcaag ttggtcttgg
      781 tataatcctt attattacga ttattggtgg tatcgtgatc tatattacgg ctggtgggga
      841 ccaagatatg atctttacgg atactggcca tcaggtggct ggtacagtgg accttactgg
      901 gttgtaccgg tagaacgtca cttgtatggc agaagagatt ctgataattc caatcctaat
      961 caaaggcata gcggaattaa ctcttccgga agttatacca gaatgtccaa ggcagaaatc
     1021 agccaaataa ggggcagttc tgtttcaaat tccggtaaca gaccttcaca gagtgtttac
     1081 agaagagaaa acagccaaga tcgttttaca acaagtagtg gaaaagttat taatcagagc
     1141 tcagggaact caaacattgt cagaaacgct aattctggaa atactggcat aaagagtagc
     1201 gctcattcaa cttcaccaaa gaagtctgaa tatacaagat caaccggaaa ttatagagtc
     1261 ccggtacgtc aaaataatgc ggcatccggc tctaatagtg gttctggaat ttctaccggt
     1321 aaaaattcat ctacaaaccg ttctgaattt caaagaagta atattaacaa tacttattca
     1381 cagccaacta gaaattcggg aacatttcag agttcaggtt cttctactca atcatctcaa
     1441 tcatccggta gtgggagaag ttcttcaggc agtggatcag tttatcgtag ataatttatt
     1501 atgaaaaaga catttacatt tacagttgcc attttactta ctactttact atcggcacaa
     1561 aatagatatg atgctttaag gtattcccaa aatttttatg aaggaagtgc aaagagctta
     1621 gctatgggaa acgcgattgt ctctatcggt ggagatcttg gagctatcag tataaatcca
     1681 gcttcagcag ggctttatag atactctgaa tttatgttta ccccatccac tatttcaact
     1741 cttactgaaa ccgactatct ggacagaaat acagacaagc gttttttcag ggctggggtt
     1801 tcttcctttg gttttgtcac tccgcttaac ggcataagaa agacaggcaa tcttaaagga
     1861 ttgaatgtgt ctgtatccat gaataaactg aataacttta ataaccgggt atcggcaaaa
     1921 ggacgcgctg acaacaccag ttggctctca tttcttgcat acaatacaaa aggaatcaac
     1981 tcatcagagt tggatattca aagtgtaggc gatacatatc ctttttacaa cagtggtgca
     2041 tcttggagtt cagttctagc atggaatacc aatctgcttg acaggctacc tgattccgaa
     2101 agtaaatata ttgctgcaac agaaaatatt gtgggtaatt ctaacgtgat tgggggccct
     2161 cttgatcagg ggtttaaaag agaaaccact ggaggaatat cagaatatgc tattactctt
     2221 agcggaaatg ttaacaataa gttttacttt ggattcagcc tgggactaca gtccttgaga
     2281 tatacagact ggcagtctta cagtgagttt gcagtgaatt taaatcagtt tgattcaaag
     2341 ttcaaagatt ttgaacatat ttacaagcag actacaaccg gcgttggcat aaatgcaaga
     2401 gcaggcgtta tttacgttcc tgttgaaggt ctaagactgg gtgccagcct ggctactcct
     2461 acatatttct cgctggaaga tgaatggaga gaaagcatgt acgctgaata cagtgataat
     2521 tatcatgtct ttttaaaatc tccaaagggc tcttacgact acttggtgat tacacctgca
     2581 agggcaaatc tgggcatatc ttattttttc ggaacaagag gatccatatc tcttgattat
     2641 gagtttgtcg gctacgaatg gatgcggatg gatgatgctg actctgattt tgatgagttt
     2701 gataatgaaa atgatttgat agaggaggat tttcaaaaca ctagtaatat tagggcaggt
     2761 gctgagttta ggttaaacag aattttttct ttgagggcag gatattcatt ctatcaaaat
     2821 cctgagagaa attatggatt tgacacacag tttgtatcat gtggtgccgg atttaaattt
     2881 ggaagtgggt tttttgccga ttttggtatt cagagacgaa tctctaattc agaaaatttc
     2941 aggctttatg aaaatgttcc tttgcaggga gggtcatcat tattggctcc aacaggcgaa
     3001 tcaacatata ctgcttggaa aatgctggca actataggct ttaggtttta gaaaaagaat
     3061 gtgatggagt taggcccttc gttaaagagg aggtctatta ttgagagatt cggaatgaat
     3121 ccatatttat gggcaaatac ctgatagtat tgcccatatt tattattaaa aagcacttct
     3181 ttttttggat gaaaataatt tctgtaatca ttcccttcaa aatctcttat ataattttgt
     3241 gtgaattcag gtactgatga tattccaatc agatcaagaa tagtgtttaa aagtctatta
     3301 ttatagttaa acagcaaagg ttcttcttca gtataaaatg agtatatttc atccttgtaa
     3361 taacaaaaaa aaggagagga gttataggca gaaaatatgg ctctccaatg ttgtttttgc
     3421 cactttttgg tataatctat ctttaccttg tcaattcttc ttgtatcttc gtcattcctg
     3481 tcttctcgac atatgggtat tatcagtggt aaaagcccgg ctgaactgta tatataacaa
     3541 cgattcctgt aggattgctt ttgatagttc tcgtatcttt caattacagg cctgccggag
     3601 ttagctattg ctcttatata ttcaatcgaa ggaagatacg cagttgataa caaaaccgaa
     3661 gcagaatgac tgttatccat gtataagtct cgaaatttta ggaactatgc ctcttttgga
     3721 attcttgata aaatcaagaa tggttctttt ctcagtactc tcttcaaaat cctgttctat
     3781 tctttcacag gcatctgaaa tattgaggcc cgaatcgtat agagttctgt aaatgctctc
     3841 aattctggat atctgctcac ttgtaaaacc gtttcttctc agacccacta tattcaattt
     3901 ataaaaagct acaggattat tccctgttaa tgcataagga ggtacatcct tccttacggc
     3961 agatgctcct gataccatac tataagctcc caccctggta aactgatgaa atgcagcctt
     4021 tcctccaatt gtagcataat catttacttc aacaaagccg gcaaaaccaa catagcttat
     4081 aataactgtt ccattgccaa tcacgcagtc atgggctaca tgagaataag acataagcat
     4141 attattatta cccataactg tcttcccccg cccatgtata gtacctcttg ccatatttgc
     4201 aaattctctg ataatattgc cgtcacctat ctcaagccaa gtctcttctt cggcaaatcc
     4261 cacatcctga ggagtagagc ctaagacagc tccgggataa attttgttat tactgccaag
     4321 ttttacatag tcaagaatag tcacatgagg ccctattatc gtaccatcgc ctatctccac
     4381 attttctgca atataagaaa atggaccaac tgttacatta ttacctattc tggctttcgg
     4441 atggataaat gtattactca tacaattgtt tttcactcct tgttcttaat cacctgagcg
     4501 accatctctc cttcacaaac caaggaatca ccaacgtaga cttcaccctt catatagact
     4561 attgatctac gaatcggttg agtaagagtc attttaatga cgattacatc tcctgggacc
     4621 acctttcttt taaatctaac tttatcaatt tttgcaaaat aggttgagta tttttcaggt
     4681 tcgtcaactt cgttgaggac taaaatacca ccaacttgtg ccattgattc aagaattaat
     4741 actcctggca taacaggttc gtccggaaaa tgaccgataa aatatccttc gttaatccca
     4801 atagttttaa tacctactac aacattttca gtcatttcaa taattctatc caccataaga
     4861 aaaggaggcc tgtgaggaag aagtttctta atcccgttga tatctacaag tggttctttg
     4921 ttagggtcat actgaggtat agcatctgac ctcatcaaat catttctcat aatttttgct
     4981 actatcgtgt ttatactatg tcctggctta tatgcagtta tgcgtgccct aatccttttt
     5041 cccgtaagag caagatctcc aattagatct acaagtttat gtcttgcaca ctcatcctta
     5101 aaccttaaac ctgattcatt gatataccct cttgaaagat cgcctttcaa gacgccaggg
     5161 taactatttt ttataaaatc cagatcttct tgggataaag gcttatctgc tataactata
     5221 gcattatcaa tatcaccacc cttaataagt ccctgtttca ggagtggcaa tatatcgtga
     5281 ataaatacaa aagtcctgca aggagctatt tcagttaaaa aatcagtatt attatcaaat
     5341 ataacacgtt gcttgccaat tatatcagag tcaaaatcaa ttgtcacatc aatcattaac
     5401 tctgaggcag gttctatcgt tatctctgag ccgcttttgt catctttgta ataaattgta
     5461 cgttcaggag tataatagtt tgaaggtgca t
//
LOCUS       DAOTQL010000112         5436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_12078_length_5436_cov_4.691321, whole genome shotgun sequence.
ACCESSION   DAOTQL010000112 DAOTQL010000000
VERSION     DAOTQL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12078_length_5436_cov_4.691321"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1374
                     /locus_tag="P5293_06745"
     CDS             70..1374
                     /locus_tag="P5293_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRR49633.1"
                     /translation="MNGNLKFISADEAVKVVKSGDHIHLSSVASAPKILIEALMRRGD
                     AGELKDVRIHHLHTEGSAPYTDAKYEGIFFHQAFFVGSNVRKNVQAGYADYIPIFLSE
                     TQKLYRSGVLPCNVAMIQVSPPDKHGFVSLGTSVDATLAAIECAEHVIAVVNKNVPRA
                     WGDAMIPTSMINFFVQCDEPLEQAHFAEPDEIESVIGKNCAELIEDGACLQMGIGAIP
                     NAVLAQLGNHKNIGIHTEMFADGVLPLVEKGVINGANKVIDKGKMVSTFLMGSQKCYD
                     FIDDNPMVKMMDVGYTNDPFVISKNPKVTAINSALQVDITGQVCADSLGTKFFSGVGG
                     QIDFIYGASLSERGKAIIAMPSTTKKGESKIAPVLTPGAGVVTTRNHIHWLVTEYGAV
                     NLYGKSLQERAKLIISVANPNHREELDKAAFERFGPHYHFIG"
     gene            complement(1416..2402)
                     /locus_tag="P5293_06750"
     CDS             complement(1416..2402)
                     /locus_tag="P5293_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49634.1"
                     /translation="MKRLFKQSLSIFVILSLFSSCAREESESTDDVEQRYIESHVKVI
                     YKDTLTKTSSGLYIINLKEGTGPAVTDTSGVYVRYSVMDFRNNYSATTYDSIAKIIGT
                     YSDTTYFGPRLFQMGDYSLMRGVEEALKLVKQGGRIKFIIPTWLSNYNYKGSAYLLSS
                     PYVYDIEVLRVVPSVAKFQIDTLEAYSEKYYGGIDSIAYGFYHKSLEEGIGDTLAVDE
                     YVSFWYVARLLDGFVVDTNIEDTARKYRIYNSSVSYTALNHTIVDIDKTTDNELVPAI
                     DKALLNMKHGGVSVVFFSSSWGYGSSSKGFGRYQPMVFWIKVVSDGNDDSDD"
     gene            complement(2414..2965)
                     /locus_tag="P5293_06755"
     CDS             complement(2414..2965)
                     /locus_tag="P5293_06755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRR49635.1"
                     /translation="MMNIKRENNRGRIIAIFCAALAIVSCSDKAEQKTLDEEIKQIDN
                     YVSSLSSSGQTVITNGLAVRIIMKEGDGESVENGDSLYFNYAGYVFSNGLGTIFDTNL
                     DSLSSKLGIDVYNRGFNVGNVVVKSGELLEGLVKGLTGAREGEYSYIVFPSSLGFGNV
                     KVGVVPAMSPLIFEIWIKNVVKK"
     gene            complement(2940..3971)
                     /locus_tag="P5293_06760"
     CDS             complement(2940..3971)
                     /locus_tag="P5293_06760"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRR49636.1"
                     /translation="MMNDFLAKEQVDFFSQLISRANEIVILGHTNPDGDAIGSVVAMS
                     RFLKLSRKNINVIIPNKYPDYLSFLDKNGEILVFSNCKERSSDLISKCDLLLALDFNQ
                     LSRIDDMEGLVRECEASKILIDHHPSPEIDFFDLVISKIETSSNCEILYWLFYSLSGG
                     NIPQEVAEPLYVGMMTDTNNFANSVFSSTFRMASDLMEKGVDKEKLQYLVFGGFSEAR
                     MRLMGYLMSEKMVILYGLEAGYIMLSKCDQNRFGFNDGDSEGFVNIPLSIKGINISAL
                     FTEGDNYVRVSLRSKNDFSVNSMARRFFNGGGHERAAGGKLFIPFDQVEKYFIESLKI
                     CYDEYKKGE"
     gene            4075..4680
                     /locus_tag="P5293_06765"
     CDS             4075..4680
                     /locus_tag="P5293_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49637.1"
                     /translation="MKNILTYFVLPVIVVLLGYLIYRSLRAPVEFNQDKAYREQIAIE
                     RLKDIRTLQTAYKTKYGKYTASFDTLLNFYNNDHITLVKQIGSMDDSIAVAQKKVFRD
                     SIKIAVRDTLLKRPGFVIDSIQYIPFSGKQKFEMKAIVAKVSGVNVPLFEACAPYDLL
                     LKGLNRQYIVNLNYERTSSDRYPGLKVGSIDAPNNNAGNWE"
     gene            4684..>5436
                     /locus_tag="P5293_06770"
     CDS             4684..>5436
                     /locus_tag="P5293_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRR49638.1"
                     /translation="MPVIVTNQPELGKTAEKRLSIQADLNGFSFCVTDILRNKLLYLF
                     KSNFSWDRAHTDIFIKHVDDCIKSHPVLKQQYNSVDFIYDTPKYVLIPKHLYKKGEEL
                     QYITKLYQLEDVEEIDIIEDLEKEIVILFAVNSTILNLLREFQPSFRIFPAIFPVMKH
                     LINFNEINKIFFKYNKGETSIIAFEGKRLLFCNNFPAQHFNTALYYLFLAVKQIQFNP
                     EQTTIFVSGSIPEIELMNMTKYFSKIRYFRNSE"
BASE COUNT         1858 a         1130 c          896 g         1552 t
ORIGIN      
        1 tttcatacat ttgccccttg cataaaatta aaaacaatat tagaaacatc taaatatcag
       61 caatttaaca tgaatggcaa tttaaagttt attagcgcag acgaagctgt caaagttgta
      121 aaatcaggag atcatatcca tttaagtagc gtggcaagtg cgcctaaaat tctcatagaa
      181 gctctaatgc gcagaggaga tgccggagaa ctcaaagatg tacgtattca tcatcttcat
      241 acagaaggtt ctgctcctta tactgatgca aaatatgaag gaatcttttt tcaccaggct
      301 ttttttgttg gaagcaatgt acgtaaaaac gtccaggcag gttatgccga ttatatacca
      361 atttttttga gtgaaacaca gaaactatac agaagtggag ttcttccttg caatgttgca
      421 atgattcagg tatcaccccc ggacaaacat ggtttcgttt cattaggaac atctgtggac
      481 gcaacacttg cggccatcga atgcgcagaa cacgttatcg cagttgtcaa caaaaacgta
      541 ccaagagcat ggggagatgc aatgattcca accagcatga tcaatttttt tgttcaatgt
      601 gatgaacctc tcgagcaagc acatttcgca gagccagacg agattgaatc ggttataggt
      661 aaaaactgtg cagaattgat agaagacggg gcttgtctcc aaatgggaat cggtgccatt
      721 ccaaatgcag ttcttgcaca gcttggcaat cataaaaaca taggcattca caccgaaatg
      781 tttgccgacg gtgtcttgcc acttgtagaa aaaggtgtga taaatggtgc aaataaagtt
      841 atcgacaaag gaaagatggt ctctacattt ttaatgggat cccagaaatg ctatgatttt
      901 attgacgaca atccaatggt aaaaatgatg gacgtaggat atacaaatga tccgtttgtg
      961 atttccaaaa atccaaaagt tactgctata aactcagctt tacaggtaga catcactggc
     1021 caggtttgtg ccgattcgct tggaaccaaa ttcttttctg gagttggagg acagatagac
     1081 ttcatatatg gcgcttcgct ctcagaaaga ggaaaagcca ttattgccat gccatcgact
     1141 actaaaaaag gcgaaagcaa gattgcacct gttctcactc ccggagccgg agttgtaaca
     1201 acaagaaacc atattcactg gttagttaca gaatacggag cagtcaatct ctacggcaaa
     1261 tcattgcagg aaagagccaa attaataata agcgttgcaa atcccaatca tagagaagaa
     1321 ctagacaaag ctgcctttga acgctttggc cctcactatc actttattgg ataaataaca
     1381 tcttggacga ttaaaaaatg tcaacacaga caaatttagt cgtccgagtc atcattaccg
     1441 tctgaaacaa cttttatcca gaacaccatc ggttgatacc ttccgaaacc cttagaagag
     1501 gatccgtatc cccagcttga agagaaaaac acaaccgaga caccaccatg tttcatattc
     1561 aacaaagcct tatcaattgc aggaactaac tcattgtcag tggtcttgtc aatatctaca
     1621 atagtgtggt tgagagctgt ataggagaca gaagagttat aaattctata ttttcttgca
     1681 gtatcttcaa tattggtatc aaccacaaat ccatcaagta atcgtgctac ataccaaaaa
     1741 gagacatact catcgacagc aagagtatca ccaataccct cttcaagaga cttatgataa
     1801 aagccataag caatagaatc tatcccacca taatatttct cactatacgc ctcgagagtg
     1861 tcaatctgaa atttggccac gcttgggacc accctgagaa cctcaatgtc atacacataa
     1921 ggtgaagaaa gaagataagc actaccttta taattatagt ttgaaagcca tgttggaatg
     1981 ataaacttaa tccttccgcc ctgtttcacc aactttaaag cctcttcaac tcctctcata
     2041 agtgaatagt ctcccatttg gaaaagtctc ggaccaaaat atgtagtatc agaatatgtg
     2101 cctataatct tagcaataga atcatatgta gtggcagaat aattattcct aaaatccatg
     2161 accgaatatc tgacataaac accggaagta tctgtaaccg caggacccgt accctccttg
     2221 agatttataa tgtatagtcc agaagaagtc ttggtcaatg tatctttata tataacctta
     2281 acgtgcgatt caatatatct ctgttcaaca tcatctgtac tttccgattc ctctcttgca
     2341 catgaagaga aaagtgacag tattacaaaa atagacaaac tttgtttaaa tagtcttttc
     2401 atcaatatca tatttatttc ttaaccacat tcttaatcca aatttcaaaa ataagcggag
     2461 acatagcagg cacaactcca actttaacat tgccaaaccc taacgatgaa gggaaaacga
     2521 tatatgaata ttccccctcc ctggcacctg taagtccctt cacaagtcct tccaaaagtt
     2581 ctccactctt cacaacaaca ttccctacat taaagcccct gttatagaca tctatcccca
     2641 atttagatga taacgaatcc aaatttgtgt caaaaattgt gcccaagccg ttagaaaaga
     2701 cataccctgc ataattgaaa taaagagaat ccccattctc aactgactca ccatccccct
     2761 ccttcattat tattcttaca gccaaaccgt ttgttatcac tgtttggcca ctgctactta
     2821 atgaagaaac ataattatca atctgcttaa tctcttcatc gagagttttc tgctcagcct
     2881 tgtcagagca tgatactatt gctaaagcag cacaaaatat tgctataatc ctacctctat
     2941 tattctccct ttttatattc atcataacat attttgagag attcaataaa atacttttca
     3001 acttggtcaa aaggaataaa taattttcct cctgcagccc gttcatgccc tcctccatta
     3061 aaaaaacgtc tcgccatact attcacggaa aaatcatttt ttgaccttag agaaaccctt
     3121 acataattat caccttcagt aaataaagct gaaatattaa tacctttgat ggacagggga
     3181 atatttacaa aaccctctga gtcaccatca ttaaagccaa atctgttttg atcacacttt
     3241 gagagcataa tataacccgc ctcaaggcca tacaaaatta ccatcttttc ggacatcaaa
     3301 taacccataa gacgcatcct tgcctcacta aaacctccaa agaccagata ttgaagcttc
     3361 tccttgtcga cacctttttc catcaaatcc gatgccatcc tgaaagtaga tgaaaaaact
     3421 gaattggcaa aattattggt atctgtcatc attcccacat agagtggctc agctacttcc
     3481 tgaggtatat tgcctccaga cagtgaataa aagagccaat aaagtatttc gcaattagaa
     3541 gaggtctcaa tcttagaaat aacaaggtca aaaaagtcta tctccggtga tggatgatga
     3601 tcaattaaaa ttttggaagc ctcacattcc cgcacaagac cttccatatc atcaattctg
     3661 gataactggt taaaatccaa agcaagcaat aaatcacact tagaaatcaa atcggaagat
     3721 ctctccttac aattcgaaaa tactaatatc tcaccatttt tatctaaaaa agaaagatag
     3781 tcaggatact tgttgggaat tattacattt atgttttttc ttgaaagttt aagaaaacga
     3841 gacatggcga caaccgaacc aatagcgtca ccgtcaggat tggtgtggcc caatataact
     3901 atttcatttg cacgtgatat tagttgtgaa aaaaaatcaa cttgctcttt tgctaaaaaa
     3961 tcattcatca ttcaaaactt gtcacaaaaa tataaaatat attgcggggt ctgaatattt
     4021 gtcttacttt tgtagaactt atatgaaata tttaaaataa aattcataaa cccaatgaaa
     4081 aatatcttaa catactttgt tctgcctgtc atcgtcgtat tactgggcta tttgatatac
     4141 agaagtctcc gggcaccggt tgaattcaat caggataaag cctatcggga acaaatagct
     4201 attgagagac tcaaagacat ccgcacactc caaactgcct acaaaacaaa atatggcaag
     4261 tatactgcca gttttgacac attgctaaac ttctacaata atgatcacat tactctcgtg
     4321 aagcagattg gatcaatgga tgactcaata gcagttgctc agaaaaaagt tttccgagac
     4381 agtattaaga ttgcagttag agatacactt ctcaaaagac cgggttttgt cattgactca
     4441 atccagtaca tccctttcag cggaaagcag aaatttgaga tgaaagcaat tgttgccaaa
     4501 gtatccggag ttaatgtgcc tctctttgaa gcttgtgctc catatgatct tttgctcaaa
     4561 gggttgaatc gtcaatacat tgtcaatctc aactatgaga gaacctccag tgaccgttac
     4621 cctggtctca aagtcggcag tattgatgct ccaaacaaca atgcaggaaa ctgggaataa
     4681 aaaatgccgg tcattgttac aaaccagccg gagttgggga aaactgcgga aaaaagatta
     4741 tccattcagg ctgacttgaa tggattttct ttttgtgtta cagatattct ccgaaataag
     4801 ctgctttatc ttttcaaatc aaactttagc tgggatagag cacatactga tatatttatc
     4861 aagcacgtag atgattgcat taaatctcac cctgttctta aacaacaata taatagtgtt
     4921 gactttatat acgatactcc taaatacgta ctcatcccca aacatttata taaaaaaggc
     4981 gaagagctgc aatatattac caaactctat cagctggaag atgttgaaga aatagatata
     5041 atagaagatt tggaaaaaga gattgttatt ctttttgcgg taaacagtac tattttaaat
     5101 cttttaagag aattccaacc ttcattcaga atatttccgg ccatatttcc agtaatgaag
     5161 catctgatca acttcaatga aatcaataaa atattcttta aatacaacaa aggagaaact
     5221 tcgataatag catttgaagg aaaaagactg cttttttgca acaatttccc tgcacaacac
     5281 ttcaacacgg ctctttacta tctttttctt gcagtaaagc aaatacagtt caatccggaa
     5341 cagacaacaa tttttgtcag cggttcaatt cccgaaatag aactgatgaa catgacaaaa
     5401 tatttttcga agattagata cttcagaaat tctgaa
//
LOCUS       DAOTQL010000113         5418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_12117_length_5418_cov_4.163528, whole genome shotgun sequence.
ACCESSION   DAOTQL010000113 DAOTQL010000000
VERSION     DAOTQL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12117_length_5418_cov_4.163528"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="P5293_06775"
     CDS             complement(<1..220)
                     /locus_tag="P5293_06775"
                     /EC_number="1.1.1.272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Involved in the metabolism of aromatic amino acids;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRR49639.1"
                     /translation="MKIVFLDQATMGDDISFKPFEELGDYHGWPLTSPDEVVSRISDA
                     EVVIVNKVVIGKPEIDAAPRLKLICVAAT"
     gene            complement(273..356)
                     /locus_tag="P5293_06780"
     tRNA            complement(273..356)
                     /locus_tag="P5293_06780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Leu,seq:gag)
     gene            447..1019
                     /locus_tag="P5293_06785"
     CDS             447..1019
                     /locus_tag="P5293_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49640.1"
                     /translation="MNENFRKQISNIEDLVEISEILSESTVLLESISKYMKHPFDPQF
                     SKDGHHIPMNFDKKGDTIYLLGEYHDRDEEADNSSNIEILYDAIEHKLVTSAHQLKGK
                     GLFCGLVEACAIKKLGFDITSDSEMPEKDFLFDDRNPAILVSVDGNQEDKFVDYMFNN
                     GVRLTLLGHVTKGEMRLDDLSFGFINSFIE"
     gene            1019..1738
                     /gene="ubiE"
                     /locus_tag="P5293_06790"
     CDS             1019..1738
                     /gene="ubiE"
                     /locus_tag="P5293_06790"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRR49641.1"
                     /translation="MKKELSKEYSKISSMFNSISGKYDFLNHLLSFGIDKTWRNKLVR
                     RLKNIPSRSKVLDIGCGTGDLTIALYKAGFETVGLDIADKMLKIAQKKSSCLNKKDIP
                     NPQFFEGNAENLPFGDYVFDAVTISFGIRNFDNREKALKEIFRVLKNNGKLAILEFSE
                     PKNRLWRNLYKIYFTGLLPFIGRLISKDTEAYKYLPDSVRDFPQYEDFCNEISMTGFS
                     NIIYKPLTGGIATLYIADKLG"
     gene            1753..3207
                     /gene="cls"
                     /locus_tag="P5293_06795"
     CDS             1753..3207
                     /gene="cls"
                     /locus_tag="P5293_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRR49642.1"
                     /translation="MVLYSVYYLLAVIFSASIMYKRLDPVKSLSWIVVILFLPYIGLF
                     LYLFFGQNFRKTKIYSRKGVRDERLRRLMSHRQMKLFRDSPESVPAELHKFRKLIFLN
                     LNGNRSVVGKNSNVETFFSGKDALDSMYNSILEAKQHIHLQSYIIVDDTTGNRFKSLL
                     ISKAKEGVEVRIIYDYVGSWHLKKEFVEEMQQAGIEVLVFAPVRFPTLTSKVNYRNHR
                     KILIVDGYIGFLGGVNIADRYYDGGNFIEWRDTHIKIMGESVASLQSSFLLDRYFILH
                     KQLKRKKKYFPSVHPAGYNQSEEIEKPIYAQIITSGPDSDWASIMQCYFQAIAEAKKN
                     IYIITPYFTPNASLLDALKIAALCGLDVSIMIPEKSDTVLGHWGTMSYAAELLEAGVK
                     VYIFRRGFNHSKVISIDGEFCIIGSANFDNRSLEHNFEITSIIYNRYVTEKIDHQFIK
                     DIERCKSLASSKWAKRSVKNQIKEAFARLLSPLI"
     gene            3373..>5418
                     /locus_tag="P5293_06800"
     CDS             3373..>5418
                     /locus_tag="P5293_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRR49643.1"
                     /translation="MLRKSIKLTLLLLLLAGSINAQIKNPVKWKIEYKESGNNVYDII
                     FNATIEEGWHLYDLGPYNDGPNPTQFYFDESKSYKRVGNVKMLIQPKVEFDEMFQMKI
                     GYFLNRAQFAQSIQVLSKDSVTVKGYVEWMVCDNGSCLPPTEYEFAVKLPGAKGTVAT
                     AIDTKSTGNAVPKSASAAVTTTDNTTATDKKEESIAQLDTSTITPVEAGISATIGDSN
                     QKKSIWGVVLEAILWGFIALLTPCVFPMVPMTVSFFLKNSQAEEGKSSKSKGKIMASF
                     YGLFIVALYTLPIAIIILVTYFAGGEAVTADIFNWLATHWLPNIIFFLVFMIFAASFF
                     GAFEITLPSWMVNKSDTKSDKGGLIGVFFMALTLVLVSFSCTGPIVGTILIKSTQGEI
                     WEPIITMFAFSVAFALPFTIFAFAPSLLKDLPKSGGWLNSVKVVLGFIEVALGFKFLS
                     VADQTYHWGILDREVYLAIWIVVFTLLGFYLLGKIRFAYDTPIDHIGVGRLTLSIIVF
                     TFVVYMIPGMWGAPLKALSGYMPPQSSQDFNLYQERHAGAGDGEDLTSITNNTQPPFT
                     AKYSEFLHLPHGLQGFFEYDEAIAYAKKVGKPLFVDFTGHGCVNCREMEARVWSDPRV
                     LKLLREKFVILALYADDKKEADEKDWVTNESGRVLKSIGKINSHLAMTKFNVNAQPYY
                     "
BASE COUNT         1753 a          986 c         1022 g         1657 t
ORIGIN      
        1 cggtagcagc cacacatatt aacttgagtc ttggagcagc atcaatttca ggtttgccta
       61 ttacaacttt attgactata accacctctg catcagatat tcttgacacc acttcatctg
      121 gagatgttaa cggccagcca tggtagtccc ctaactcttc aaaaggctta aatgagatgt
      181 catctcccat tgtagcctga tctaaaaaaa ctatcttcat aaaaccaaca aagccgtcta
      241 ctaacggctt tcaaattttg ttaaatttaa agtgcccaga acaagactcg aacttgcaca
      301 ccgaaaccgg cactaccccc tcaaagtagc gtgtctacca attccaccat ctgggcttgt
      361 gattgggact gcaaatatac tctctttttt tatttttaaa aatttttaaa ttattcgtaa
      421 cttagcatca aataataaaa ttagaaatga acgaaaattt cagaaaacag atttctaata
      481 ttgaagatct tgttgaaatt agtgaaatat tgtcagaaag cactgttttg cttgagagta
      541 tatcaaaata catgaaacat ccttttgatc cacaattttc taaggatgga catcatattc
      601 caatgaattt cgataaaaaa ggtgatacta tctatcttct cggagaatat cacgacaggg
      661 acgaagaggc agataatagc tcaaacattg agatccttta cgatgccatt gaacacaagc
      721 ttgttacaag tgctcatcaa cttaagggta aaggcctttt ttgtggtctg gtagaggcat
      781 gcgctatcaa aaagctgggg tttgatatca cttctgattc tgaaatgcct gaaaaagatt
      841 tcttattcga cgatcggaac cctgccattt tggtgagcgt agatggcaac caggaggata
      901 aatttgttga ttacatgttc aataatgggg ttaggctgac tcttctcggt catgtcacaa
      961 aaggagaaat gagacttgat gatctctctt ttggattcat aaacagcttt attgaataat
     1021 gaaaaaagag ctgagtaagg agtactcaaa aatttcctca atgttcaact caatttcagg
     1081 caagtatgat ttcctaaatc acttgctctc ttttggaatt gacaaaactt ggagaaataa
     1141 gcttgttaga agattgaaga atattccttc acgcagcaaa gtactcgata taggatgtgg
     1201 aaccggcgac cttacaatag ctttgtataa agcaggattt gaaacagtgg gtttggatat
     1261 tgcagataaa atgttgaaaa ttgcccaaaa aaaaagtagc tgtcttaata aaaaagatat
     1321 tccaaaccct caattttttg aggggaatgc tgaaaatctt ccttttggtg attatgtttt
     1381 tgatgcggtc acaatctcat tcggaattag aaattttgat aacagggaga aagctttaaa
     1441 agagatcttc agagttctca aaaacaatgg taaacttgct attcttgagt tttctgaacc
     1501 caaaaacaga ctttggagaa atttatacaa aatctatttt accggactat taccatttat
     1561 tggaagacta atttccaagg atacagaagc atataaatac ctccctgact ctgtaagaga
     1621 ttttccacaa tacgaggatt tttgcaacga aataagcatg actgggtttt caaatattat
     1681 ttacaaacct cttacaggag gaattgctac attatatatt gcggataaat tggggtaact
     1741 gagatcatat cgattgtttt gtattcagta tattatcttc tggcagttat cttttcagcc
     1801 agcataatgt acaagagact tgaccctgtt aagtctcttt cttggatagt tgtcatactg
     1861 tttcttccat atatcggact tttcctctat ttgttctttg gacaaaactt tcgtaaaacc
     1921 aaaatataca gcagaaaggg agtcagagac gagagactga ggagattgat gagccataga
     1981 caaatgaagc ttttcagaga cagtcccgaa agcgttcctg ccgaactgca taaatttaga
     2041 aaactgattt ttctgaatct taacggtaat agaagtgtcg tcggaaaaaa cagtaacgtt
     2101 gagacattct tctccggcaa agatgcactc gactcaatgt acaactctat tcttgaagca
     2161 aagcagcaca tacatcttca gtcatacatt attgtagatg ataccaccgg taatagattt
     2221 aaatctcttc tgatctccaa ggcaaaagag ggtgttgagg tcagaataat atatgattac
     2281 gttggaagct ggcatctcaa aaaggaattt gttgaagaga tgcaacaggc aggcattgaa
     2341 gtcttagttt ttgctcccgt aaggtttccg acacttactt caaaagtcaa ttatagaaat
     2401 cacaggaaaa ttcttatcgt agacggatat attggatttc ttggcggtgt aaatatagcc
     2461 gacagatatt atgatggagg taattttatt gagtggcgcg atactcatat caaaattatg
     2521 ggagaatctg ttgcctcact tcagtcaagt tttttgctcg acaggtattt cattcttcac
     2581 aaacagctta agcgcaaaaa aaaatatttc ccttccgttc accctgcagg ttacaatcaa
     2641 tctgaagaga ttgaaaaacc tatttacgct caaatcataa cttcaggacc tgacagcgat
     2701 tgggcaagca tcatgcagtg ttattttcaa gcaatcgctg aagccaaaaa aaacatctat
     2761 attattacgc cttactttac tcctaatgca tctctcctcg atgccctaaa gatagctgcc
     2821 ctttgtggat tggatgtttc aataatgatt ccggaaaaaa gcgacacagt tttaggccat
     2881 tgggggacta tgtcctatgc agccgaactt cttgaagccg gagtaaaagt ttacattttc
     2941 agaagaggat ttaatcattc aaaagttata tctattgatg gagaattttg cataataggt
     3001 tctgccaatt ttgacaacag gtctcttgaa cacaattttg aaattaccag tataatctat
     3061 aatcgatacg tcactgaaaa aatagatcac caatttatca aagacataga aagatgcaaa
     3121 tctctcgcaa gttcaaagtg ggctaaacga agcgtgaaaa atcaaataaa ggaagctttt
     3181 gccagacttt taagtccact tatctagcta aaagttagcg caatctgaaa atatttacat
     3241 tattttgata ttttatttgg ggtaaatgcc tataaaagtg tatttaatat aggaaaaaca
     3301 ttttaaacct ttttttatta attgcgtcaa aaagcatcca ttattaatat atttggaaaa
     3361 ataattaatc atatgttgag aaaaagtatc aaattaacac tgctgctctt attgcttgct
     3421 ggaagcatta atgcccaaat caaaaaccct gttaaatgga aaattgaata taaagaaagt
     3481 ggaaataatg tctatgacat tatattcaat gcgaccattg aagaagggtg gcatctttac
     3541 gatctaggac cctacaacga tggccccaat cctactcagt tctattttga tgaaagtaaa
     3601 agttacaaga gagtcggaaa tgtaaaaatg ttaattcaac caaaagttga gtttgacgaa
     3661 atgtttcaga tgaaaatcgg atattttctc aacagagctc aatttgcaca gtctatccag
     3721 gttttatcga aagattctgt tactgttaag ggatatgttg aatggatggt gtgcgataat
     3781 ggttcatgcc tgccccctac agaatatgag tttgcagtaa agcttccagg agccaaaggg
     3841 actgttgcga cagcaataga tactaaatca acaggtaatg cagtacccaa gtctgcaagt
     3901 gctgctgtta caacaacaga caacacaaca gctacagata aaaaagaaga gagtattgct
     3961 caattagata catcaacgat aactcctgtt gaggctggca tatcagcaac aattggtgac
     4021 agtaaccaga aaaaatccat ttggggagtt gttctcgaag caatcttatg gggttttatt
     4081 gccctgctaa ctccatgtgt cttccctatg gtgcccatga cagtatcatt ttttcttaaa
     4141 aacagtcaag ccgaagaagg aaaaagttcc aaatcgaaag gcaaaattat ggcctctttt
     4201 tatggcttgt ttatcgttgc actttataca ctaccaattg caataattat acttgtcacc
     4261 tattttgccg gcggagaagc tgttacagca gatattttta actggctggc gactcattgg
     4321 cttccaaata ttatattctt cctggtattt atgattttcg cagcttcatt ttttggcgcc
     4381 tttgaaatta ccttaccaag ttggatggtc aataaatcag ataccaaatc tgacaaggga
     4441 ggattaattg gagtattttt catggccctt acacttgttc ttgtatcttt ctcatgtacc
     4501 ggacctattg taggtacaat actcatcaag tctactcaag gagaaatatg ggaaccaatt
     4561 attacaatgt ttgcattttc agtggcattt gctcttccct tcacaatatt tgcctttgcg
     4621 ccttcactcc ttaaagatct tcctaaatct ggcggatggc taaactctgt gaaggtagta
     4681 ttaggtttta ttgaagttgc gcttggattc aaatttctta gtgtcgcgga tcagacttat
     4741 cattggggca tactcgacag agaggtgtac cttgcaattt ggattgtagt atttaccttg
     4801 cttggctttt atcttcttgg caagataaga tttgcttatg acactccaat tgatcatatt
     4861 ggcgttgggc gtctgacact ctcaattata gttttcactt ttgttgttta catgataccg
     4921 ggaatgtggg gagctcctct caaagctctt tcaggctata tgccgccaca gagttctcaa
     4981 gacttcaatc tctatcagga aaggcatgca ggagcaggag atggcgaaga tttaacaagt
     5041 ataacaaaca atactcaacc accttttact gcaaaataca gtgaattttt acatcttcca
     5101 catggtcttc aaggtttctt cgaatatgac gaggctattg catatgccaa aaaagtagga
     5161 aaaccacttt ttgttgattt tacaggacac ggttgtgtaa attgtcggga aatggaagca
     5221 agagtgtggt cagacccaag agttttaaaa ctgctaagag agaaattcgt gatactcgca
     5281 ctttacgctg atgataagaa ggaagctgat gagaaagact gggttacaaa cgaaagcggt
     5341 cgcgttctca aaagtatagg taaaatcaac tcccatcttg caatgactaa atttaacgtc
     5401 aatgcacaac catattat
//
LOCUS       DAOTQL010000114         5399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_12165_length_5399_cov_4.994573, whole genome shotgun sequence.
ACCESSION   DAOTQL010000114 DAOTQL010000000
VERSION     DAOTQL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12165_length_5399_cov_4.994573"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="P5293_06805"
     CDS             complement(<1..549)
                     /locus_tag="P5293_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRR49644.1"
                     /translation="MKRLWGFTALCSMVLANLTLSPAMAQVPSNSKHLKVSIIPEPVK
                     VVPGINSYRINKNTVIVASGEEQLKSAKFLKDYILKYYNVELAIIPARDANGKEIKIK
                     KNKEAEIKHVVLDIKPLAYGIAGFSPSDLKKIGAYTLNTKDGILNLTGANPEGLFYAV
                     QTLIQLLPSTVPQLGLFDKYLQI"
     gene            complement(546..965)
                     /locus_tag="P5293_06810"
     CDS             complement(546..965)
                     /locus_tag="P5293_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49645.1"
                     /translation="MSSTNIKGRKILVKQPAEVLFAMFSDLSNFSKSVPADVLKGNEL
                     IATQDTVVTKVQGFEIGLKVAERVPFSLIKYEQYGNSPFPFVFFITFDERCTGETELM
                     IELETELSGMLKMMLGGKLKELVDKVTNELELNLNRK"
     gene            complement(985..1617)
                     /gene="pyrE"
                     /locus_tag="P5293_06815"
     CDS             complement(985..1617)
                     /gene="pyrE"
                     /locus_tag="P5293_06815"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRR49646.1"
                     /translation="MDLVEEIVAKLLLDIEAVRLNPDAPFTWASGWRAPIYCDNRKIL
                     SFPEQRKIIYDNLTKLVRENYPDAEVIAGVATGAIAWGALVAEMLNLPFIYVRPIPKD
                     HGTGAQIEGYLPDNASVVVVEDLISTGASSLSAVSCLNRSGANVLGMVAIFSYNFDVA
                     RRSFEYANCELRTLTNYDMLIEQALKSGYIKEEMMETLRQWKYRPEAWGK"
     gene            1741..2352
                     /locus_tag="P5293_06820"
     CDS             1741..2352
                     /locus_tag="P5293_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRR49647.1"
                     /translation="MTVQHLLEDPSTVHFFPGNDQKTEELPFLFTKLTNIEKLMILTS
                     DQEATLDQLCKKLIKITAGGGLVTNNEGELLLILRDNLWDLPKGKQEDGEDIETTALR
                     EVEEECGIKDLQIKNKICETYHTYMNRDNNLTIKCTYWFHMEYHGKKTKTTPQKEENI
                     EQIVWIKPTELPQYLNNTYESIKELFAEATTQLSIFQSQTAEN"
     gene            complement(2361..3062)
                     /locus_tag="P5293_06825"
     CDS             complement(2361..3062)
                     /locus_tag="P5293_06825"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRR49648.1"
                     /translation="MLRFTGLLKRYLVVAVLGFAGAFIFISSNSAKCANSLVGDLQSQ
                     SEKTENIISQFRSQLSQLPAFEMGFSMNYDGQVSVGTVQAQKDFYRMDNKDLSLYCDG
                     DTKWIYNVANNEVIILKNDLSQVDLLENPLAFFYSLGKGYSYPEKVKSSNVNKVPVWV
                     VDLFPINKRLGYSKISLMIDKRNYSPVRIVYVMKSGEVVTVDITSFTEKKPWEKDHFR
                     FDTAKYNGLRVSDMR"
     gene            complement(3072..3143)
                     /locus_tag="P5293_06830"
     tRNA            complement(3072..3143)
                     /locus_tag="P5293_06830"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3108..3110),aa:Glu,seq:ctc)
     gene            complement(3184..4749)
                     /gene="gltX"
                     /locus_tag="P5293_06835"
     CDS             complement(3184..4749)
                     /gene="gltX"
                     /locus_tag="P5293_06835"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRR49649.1"
                     /translation="MDSNGSNIVKRRVRVRFAPSPTGPLHIGGVRTALFNYFFAKKHG
                     GDFILRIEDTDSQRFVPGAEEYINESLRWCGIRIDEGVAEGGGYGPYKQSERRGIYRQ
                     YADMLVDSGHAYYAFDSAESLERLRKEAESRGETFNYNYKLRGLMETSLVLSQDEVRA
                     RIERGDQWVIRFMMPENEEIHMSDLIRGDVVVNSSTLDDKVLFKSSDMLPTYHLANVT
                     DDYLMEITHVIRGEEWLPSLPLHVMLYKAFGWLDRMPEFAHLPLLLKPAGNGKLSKRD
                     GDKMGFPVFPLQWKSPDGEIFRGYREDGYFPEAFVNLLALLGWNPGTEQEIFSMDELI
                     GLFSFDRVSKAGARFDPEKAKWFNHQYLVRKSNEELAGLFSSFMNSLGMEVSWEKALR
                     IVDIMKVRVNFVHEMWEKAEFFFLRPESYDEASVRKFWKEDVPAIMKEVVGVLGELEV
                     KEFVSAKIEESLENFIREKGYGMGKVMNAVRLCLIGVSNGPAVADVCEVIGREESIVR
                     IERAVEVLGNFEI"
     gene            complement(4773..>5399)
                     /locus_tag="P5293_06840"
     CDS             complement(4773..>5399)
                     /locus_tag="P5293_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRR49650.1"
                     /translation="ALARRDCVKADFVAGIPDSGIGHAIGYSHVSGIPLKRPYAKYTP
                     TWPRSFMPQSQNMRELVAKMKLIPNRAVIEGKCGIFLDDSIVRGTQLKDNVVDLLESG
                     IKEINMRIACPPLTFPCEFINFSRSRSSLDLATHKAVKRIEGSLEGVDFKEYSDFSGE
                     KYKAMVEEIRKTLGLTSLVFQRLDDLVEAIGIPKESLCTHCWDGSSYF"
BASE COUNT         1811 a         1406 c          799 g         1383 t
ORIGIN      
        1 tatctgcaaa tacttgtcaa acagacctaa ctgaggcact gttgaaggca gaagctggat
       61 cagtgtctgt acagcataaa ataatccttc gggattggcg ccggtcaaat tcaaaatacc
      121 atccttagtg ttcaaagtgt aagctccgat ttttttgaga tcagacgggc tgaaaccagc
      181 aattccataa gcaagaggct ttatatccaa aacaacatgc tttatttcag cctccttgtt
      241 ctttttaatc tttatctctt ttccattggc atcccttgca ggaattatag ccaattcaac
      301 attgtaatat ttgagaatat aatctttaag aaactttgca gatttcaact gttcctcacc
      361 ggaggcaaca ataacagtat ttttgttgat cctgtaggaa ttaatacccg gcaccacctt
      421 cactggttca gggataatag agactttcaa atgcttactg ttagaaggta cctgagccat
      481 agcaggagat aacgttaaat ttgccaaaac cattgagcaa agagcagtaa aaccccataa
      541 tcttttcatt ttctatttaa atttaattcc aattcattgg taactttatc aaccaactcc
      601 ttaagctttc cacccagcat cattttcaac atgccagaaa gttcagtttc caactcaatc
      661 atcaactctg tctcccccgt gcacctttca tcaaaagtta taaaaaaaac aaaaggaaaa
      721 ggagaatttc catactgttc atacttgata agagaaaaag gcaccctttc agcaacctta
      781 agaccaattt caaatccctg aaccttggta acaacagtat cctgagtagc aatcagttca
      841 ttgcctttca aaacatcagc aggaacactc tttgaaaaat ttgacaagtc ggaaaacatc
      901 gcaaatagaa cttctgcagg ttgtttaacc aaaatcttcc gacccttgat atttgtactg
      961 ctcatacaat caattctccc atttctactt tccccatgcc tcaggacgat atttccactg
     1021 acgcaatgtc tccatcatct cttccttgat ataaccactc ttaagagcct gctctatcaa
     1081 catgtcataa ttagtcaacg tcctcaactc acagttggca tattcgaagg agcggcgtgc
     1141 cacatcaaaa ttgtatgaaa aaatcgccac catacccaaa acgttagcgc cgctcctgtt
     1201 taaacaactc acagccgaga gagaacttgc accggtagag ataagatctt caaccactac
     1261 aactgaagca ttatcaggca agtaaccctc aatctgagca cctgtaccat gatcctttgg
     1321 tattgggcgg acataaataa aagggagatt gagcatctcg gcaaccaggg caccccatgc
     1381 aatagctcct gtagcaacac cggcaataac ctcagcatca ggataatttt ccctaaccag
     1441 cttagtcaga ttgtcatata ttatcttcct ctgttcagga aacgaaagaa tcttcctgtt
     1501 atcacaataa attggagctc tccaccccga agcccaagta aaaggagcat caggattcaa
     1561 acggacagct tcgatgtcga gtagcagctt cgctacaatt tcttcaacta aatccataat
     1621 ttcaagtatc tttgtacaaa gataaaaaat caatccatat ttttataaat gtacagtctc
     1681 ttcttcaaca accggcgtat aatcatctat tctaacaacg aaccaaaaca aagagataaa
     1741 atgactgtac aacatctcct ggaagatcct tcaacagtac acttcttccc agggaatgac
     1801 caaaaaacgg aagaactccc gtttcttttt accaaactca ccaacatcga gaaactaatg
     1861 atcttaactt cagaccagga ggccacactt gatcaactct gcaaaaaact cattaaaatt
     1921 acagcaggag gaggtttagt aacaaataac gagggagagc ttctgcttat attacgcgac
     1981 aacctttggg atctgccaaa aggcaaacag gaagatggag aagacataga gacaactgca
     2041 ttaagagaag tagaagaaga gtgcggcata aaagacttgc aaataaaaaa taaaatatgt
     2101 gaaacctatc atacctacat gaacagggac aacaatctca caattaaatg cacatactgg
     2161 tttcatatgg aatatcacgg taaaaagacc aagacaacac cacagaaaga agaaaatatc
     2221 gaacaaatag tctggataaa acccacagaa ctgcctcaat atctcaacaa cacatacgaa
     2281 tcaataaaag aactctttgc agaggccacc acccaactct ccattttcca atcacaaacc
     2341 gctgagaact aatcactctt tcacctcatg tcgctcactc tgagcccatt atacttagca
     2401 gtatcaaatc tgaaatgatc tttctcccaa ggtttctttt ctgtaaaaga agtaatatcc
     2461 acagtaacaa cctcaccact cttcatcaca tagacaatcc tcacaggaga atagttcctt
     2521 ttgtcaatca tcaacgatat tttactgtaa cccagccttt tatttatagg aaaaagatct
     2581 actacccaca caggaacttt attgacgtta gaggacttaa ctttctccgg ataagagtaa
     2641 cctttaccaa gcgaatagaa aaaagccaac ggattttcaa gcaaatccac ctgagaaaga
     2701 tcatttttca aaataatcac ctcattatta gctacattgt aaatccattt agtatcaccg
     2761 tcacaataaa gagacaaatc tttattatcc atcctgtaga aatccttttg agcctgcact
     2821 gtacccacac tcacctgccc gtcataattc atggaaaagc ccatttcaaa agccggcaac
     2881 tgactcagtt gagatctgaa ctgactaata atattctcgg ttttctcaga ctgcgattgc
     2941 aaatctccaa caagagaatt ggcacatttt gcagagtttg acgaaataaa aataaaagct
     3001 cctgcaaaac ccaaaacggc gacaaccaaa taccttttta acaaacctgt aaatcttaac
     3061 atattcgtca gtagcgcata gcagaatcga actgctattt caggaatgag aatcccgctt
     3121 cctaaccgtt agaagaatgc gccctatctc gtcaaaagtg accgcaaagg taactaatac
     3181 aaatcaaatt tcaaaattgc ccagaacctc aacagccctt tcaatccgca caatactctc
     3241 ttcccgacca atcacctcac aaacatcagc aactgccgga ccattagaca ctcctataag
     3301 acaaagcctg acagcattca taaccttgcc catgccatat cccttctcac gaataaaatt
     3361 ctcaagactc tcctcaatct ttgcagaaac aaactctttc acttccagtt cacccagcac
     3421 acccacaacc tccttcataa tagcaggaac atcttctttc caaaacttcc taaccgaagc
     3481 ttcgtcataa gattcaggcc tcaaaaagaa aaactcagcc ttctcccaca tctcgtgaac
     3541 aaagttgaca cgaaccttca taatatccac aattctcaga gccttctccc aactcacctc
     3601 catacccaaa gagttcataa aagatgaaaa aagtcctgca agctcctcat tactcttcct
     3661 cacaagatac tgatgattaa accatttagc cttctccgga tcaaatctgg caccagcctt
     3721 actcaccctg tcaaaagaga acagcccaat aagctcatcc atagaaaata tctcctgctc
     3781 tgtgccggga ttccatccaa gaagagcaag aagattgaca aaagcctccg ggaaataacc
     3841 atcctccctg tacccgcgaa aaatctcacc atcaggcgat ttccactgca aaggaaaaac
     3901 tgggaaaccc atcttatctc catcacgttt actaagcttg ccattaccgg caggcttgag
     3961 caacaaaggc agatgagcaa actcaggcat cctgtcaagc caaccaaaag ctttataaag
     4021 catcacatgc aaaggcaatg aaggcaacca ctcctcaccc ctgatcacat gagttatctc
     4081 cataagataa tcatcagtaa cgttggccaa atgatatgtt ggcaacatat cagaactttt
     4141 aaaaagcacc ttgtcgtcca gagtagaaga attcacaacc acatctcccc ttataagatc
     4201 agacatatgg atctcctcat tctcaggcat cataaaacga attacccact ggtctcccct
     4261 ttcaattctt gccctgactt catcctgaga aagcacaaga gaggtctcca tcaaacccct
     4321 caacttgtaa ttataattaa atgtttcacc acgcgattca gcctcctttc tcaacctctc
     4381 cagcgattct gctgaatcaa aagcataata agcatgccct gaatcaacca acatatctgc
     4441 atactgcctg taaatgcctc tcctttcact ctgcttgtac gggccataac cgcccccctc
     4501 agcaactccc tcatcaatcc taatcccgca ccatctgaga gactcattga tatactcttc
     4561 agcaccaggc acaaaacgtt gagaatctgt atcttcgatc cttagaataa aatcaccccc
     4621 atgcttcttt gcaaaaaaat aattaaaaag agctgtcctc acccctccta tatgcaaagg
     4681 tcccgtcgga ctcggagcaa atcgcactct aactcttctt ttgacaatat ttgaaccgtt
     4741 gctatccata accttttaat aattactaca ttctaaaaat aacttgaacc gtcccaacaa
     4801 tgagtacaca aactctcttt tggaatacca atagcttcca ccaaatcatc caatctctga
     4861 aaaacaagag aagtaagccc caaagttttc cggatctcct ccaccatagc cttatatttc
     4921 tctcccgaaa agtcagagta ctctttgaaa tcaacccctt ccaacgaccc ctctattctc
     4981 ttaacggcct tgtgagtagc taaatcaagg ctcgacctcg aacgactgaa attgataaat
     5041 tcgcacggga aagtgagtgg agggcaagca atcctcatat taatctcctt aataccagac
     5101 tcaagtaaat ctacaacatt atctttcaac tgagtacctc taacaataga atcatcgaga
     5161 aaaataccac acttgccttc tataaccgcc cggttgggaa taagcttcat cttggccacc
     5221 aactccctca tattctgact ttgaggcata aaactcctcg gccacgtagg agtatattta
     5281 gcataaggcc ttttaagagg aataccacta acatgactgt aacctatggc atgtccaata
     5341 cctgaatccg gaatacccgc cacgaaatcg gcttttacac agtccctcct tgcaagagc
//
LOCUS       DAOTQL010000115         5374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_12231_length_5374_cov_5.296860, whole genome shotgun sequence.
ACCESSION   DAOTQL010000115 DAOTQL010000000
VERSION     DAOTQL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12231_length_5374_cov_5.296860"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..558)
                     /locus_tag="P5293_06845"
     CDS             complement(64..558)
                     /locus_tag="P5293_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49651.1"
                     /translation="MPEINKVFSVREGRVSFKSGHVYRFKYLNYENDPEPTIICINAI
                     RGIHPKTRRMHNYIQGINFTYIPRSQRKVFAKVWKETLDRNKGHTLLTWKLIKRKWPW
                     MKQAIRRYILGAGYIRNPKEFLGDEMEIAIVGTWRKDFSKRMTRAREKGMKYLEAEQR
                     KFGL"
     gene            complement(561..1019)
                     /locus_tag="P5293_06850"
     CDS             complement(561..1019)
                     /locus_tag="P5293_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="gp4; gp50; gp65; in bacteriophage T4 and related
                     viruses, the virion is composed of head and tail
                     components; the virion head goes through several
                     maturation steps including the construction of the
                     prohead, enzymatic processing of the prohead, and DNA
                     packaging; gp4 is thought to participate in the final
                     steps of head maturation, prior to the attachment of the
                     virion tail; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion protein"
                     /protein_id="HRR49652.1"
                     /translation="MFIKHHFTDNTRTFQPKFPEKYKGKWPILVRSEWERKFAQWCDV
                     NPSVITWASEAVEIPYFDPINKKSRRYYPDFMLRVLDKNKKEVVYVVEIKPYRETVPP
                     KQKGNKSAKTRIYEQITYSRNQAKWTAATEFCKKRGYEFKIFTEKELFGR"
     gene            complement(1022..1216)
                     /locus_tag="P5293_06855"
     CDS             complement(1022..1216)
                     /locus_tag="P5293_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49653.1"
                     /translation="MRLEKFLKEDDIQTINARQRLKKQIQDIEKQIRNTENKETKESL
                     RKRKEALQKSLERYRESQRV"
     gene            complement(1219..1611)
                     /locus_tag="P5293_06860"
     CDS             complement(1219..1611)
                     /locus_tag="P5293_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49654.1"
                     /translation="MSKDRKNSRQLNEELISQVLMSKSWWEKSPFLKEEYKKVKKPHF
                     TTEQAKEIGEKLGIDWKKSEFDVEQFRMGMDVELEHGNINQHTNVTNDDTINTAKIAL
                     AHLNEISNYYTLLDEMEKKGKETKNPIV"
     gene            complement(1608..1808)
                     /locus_tag="P5293_06865"
     CDS             complement(1608..1808)
                     /locus_tag="P5293_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49655.1"
                     /translation="MKLKNYLIKAEDELKMKLIDFFKKNPNPSDSKVHTFADELGIEH
                     DKVEAAIYALLTSYIKDLKGEK"
     gene            complement(1808..2152)
                     /locus_tag="P5293_06870"
     CDS             complement(1808..2152)
                     /locus_tag="P5293_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49656.1"
                     /translation="MPKPREGEGKQEYLSRCIPILKGEGYDQDQAVAICYSMWRQNTG
                     KNENIDQIIDKYLVNEHFMLKPSGHRRPSQLSTSTNMKCMECGKRFKKKIGKSTYEVK
                     CPKCGGYDTEPE"
     gene            complement(2157..2444)
                     /locus_tag="P5293_06875"
     CDS             complement(2157..2444)
                     /locus_tag="P5293_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49657.1"
                     /translation="MGELIRNQILTEGGFPIVRLKQIAPNFTRINIGETVLYFSYETL
                     VAFEVDGRIYATQEKYSRTTSKHINLLNVDEYIPAEEWEQFTKKKLGNLVK"
     gene            complement(2446..3018)
                     /locus_tag="P5293_06880"
     CDS             complement(2446..3018)
                     /locus_tag="P5293_06880"
                     /inference="COORDINATES: protein motif:HMM:NF022815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HRR49658.1"
                     /translation="MPEINLKNILITMGIVFGLVSSIFALDGRYATSKELAKMEQQTV
                     QTFQQLRVDMQKSDLQQRLDRYNDRISELKLLRKKYPNDRDYEDQYMEMKRDLLERID
                     LYLINEKLWSGEVATKWEPPEGLFTSSAENIAKVLHSQSNDLKQAMSRLNFYINRAGD
                     KLSSARRRTLEDAKELLRKKFGVKKKEEEK"
     gene            3131..4126
                     /locus_tag="P5293_06885"
     CDS             3131..4126
                     /locus_tag="P5293_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRR49659.1"
                     /translation="MSFFDKVKYLDLIIDCLPDAIFAIDLQGNVIAWNKQMEKLTGCK
                     RDTVIGKGDHLYSIPFYGKKTKILIDLVFEPDPELERRYDYFERLNENVLTCHIYLEK
                     IGKTLWAKATPLKDENNEIIGAIESIRDISENTILLKKLSLSDEKFQNLYNITQLFLD
                     SINDMVWAKDIDNRFIFANKSFEKFLKKKSIDLIGRKSFDIYGQGKKFEEADEIAKTK
                     PHHIIESINIDGEEKWLDICKAPLLDRKGMLLGTIGTARDITREVELVKQRNIEIDNL
                     KKIIEEKVKDWRKENEEIHRDISKKVYESIMLLRGIQNDAKQNNIEELNKEEIFF"
     gene            4126..4641
                     /locus_tag="P5293_06890"
     CDS             4126..4641
                     /locus_tag="P5293_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49660.1"
                     /translation="MPDERLVPESIVKLLFDQIKSAADANTTSVKELGEAVFEVLRAQ
                     GNLATKKDIIDEHNLVDQKNSEKIKYIIQKIEKMEEIVGGKINGVGKTLEEEENMINK
                     IGSIETKVDNIESKIKTMITVVIVAFSVLLASFYFVKSATDVAVQSAMDRAVKEIITT
                     RPSNIYPPPSK"
     gene            4651..4833
                     /locus_tag="P5293_06895"
     CDS             4651..4833
                     /locus_tag="P5293_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49661.1"
                     /translation="MRLLNFLKKIEEENGGGAGMGGATTTANIATFAQKIPGGPTRRG
                     ALKNWKKSKKKRMFPI"
     gene            4794..>5374
                     /locus_tag="P5293_06900"
     CDS             4794..>5374
                     /locus_tag="P5293_06900"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49662.1"
                     /translation="MEKIKKEENVSYMKLIQYLIEQNIDKIKIVIITGRKNGKDSNDL
                     YRTAKRFKEACDERKIECYVAFSDDGYIARKNGKVYIHNIDDENGFEINSSNTVAIIR
                     GTTGKRKSSLDLISQLERHNIFCVNSRRTMEECSDKYRTALIMADAKIPTPKTAIILG
                     PDGIDVALKKIGKKFPVVLKTLTGSKGIGVFVA"
BASE COUNT         1698 a          989 c          865 g         1822 t
ORIGIN      
        1 attctcacgg ttggcccgta tattcctgtc attgtcaaat ctctttccat cttttatcct
       61 ccgttataaa ccaaactttc tttgttcagc ttcaagatat ttcattccct tttctctcgc
      121 tcttgtcatt ctttttgaaa aatcctttct ccaagtaccg acgatagcga tttccatttc
      181 atcaccaaga aactcctttg gattacgaat atagcctgca ccaagaatgt atcttcttat
      241 tgcctgtttc atccaaggcc attttctctt tatcagcttc caagtcaaca atgtgtgccc
      301 cttgttccta tccaatgtct ctttccatac ttttgcaaat acctttctct ggcttctggg
      361 aatatatgta aaattaattc cctgaatata attatgcatc cttcttgttt ttggatgaat
      421 accacggatt gcattaatac agattatagt tggttccgga tcattctcat aattcagata
      481 tttgaaccta taaacatgac cagacttgaa tgataccctt ccttctctta cgctgaatac
      541 tttattaatt tctggcattt ttatctacca aataattcct tctctgtaaa aatcttgaac
      601 tcgtaacctc tctttttaca aaactctgtg gctgctgtcc acttcgcctg atttcttgaa
      661 tatgttatct gctcatatat tcttgtcttt gcagacttat tacccttctg cttaggagga
      721 actgtttccc tgtatggttt aatctcaacc acataaacaa cttccttttt attcttatcc
      781 agaactctca acataaaatc tggataatat ctcctgctct tcttgtttat aggatcaaaa
      841 tagggtatct caaccgcttc ggatgcccaa gttatgacac ttgggtttac atcgcaccat
      901 tgtgcgaatt tcctttccca ttcgcttctt acaagtattg gccatttccc cttgtatttc
      961 tctggaaatt taggttgaaa tgtccttgta ttatctgtaa agtgatgttt aataaacaat
     1021 atcagaccct ttgtgattct ctataccttt ccaatgattt ctgtaatgct tcttttcttt
     1081 ttctcaaaga ttcttttgtt tctttatttt ctgtatttct aatctgtttt tctatatcct
     1141 gtatttgttt ctttagcctt tgtctggcat ttatggtttg aatatcatct tctttcagaa
     1201 atttttctaa tctcattttt atactatcgg atttttagtt tctttcccct tcttctccat
     1261 ctcatcgaga agagtataat aattggatat ttcattcaag tgagcaaggg caatctttgc
     1321 tgtgttgatt gtatcatcat tagttacatt tgtatgctga tttatattac cgtgttcaag
     1381 ctctacatcc attcccatgc gaaattgctc aacatcaaat tcagatttct tccaatcaat
     1441 accaagtttt tcaccaattt ctttggcctg ttcagttgta aagtgaggct tcttcacttt
     1501 cttgtattct tccttcaaaa agggtgattt ttcccaccac gatttactca tcaggacttg
     1561 actaatcaac tcctcattta attgtcttga atttttccta tctttactca tttctccccc
     1621 tttaaatcct ttatgtaaga tgttagaagt gcatatatag ctgcttctac tttgtcgtgc
     1681 tcaattccaa gctcatctgc aaaagtatga actttactgt ccgatggatt tggattcttt
     1741 ttgaaaaaat ctataagttt cattttgagt tcatcttcgg cttttattaa ataatttttc
     1801 agtttcatta ttctggttcc gtatcgtatc ctccacactt ggggcatttc acctcataag
     1861 tgcttttccc aattttcttt ttgaatcttt taccacattc catacatttc atattggtgg
     1921 aagtgcttaa ttgagatggc cttctatgtc ctgatggttt taacataaaa tgctcattga
     1981 caagatattt gtcaattatc tgatctatat tttcattctt tccggtattc tgtctccaca
     2041 ttgaataaca gatggcaact gcttgatctt ggtcgtatcc ttctcccttc aatatcggaa
     2101 tacaacgtga aagatattcc tgttttcctt ctccctctct tggcttcggc atattattac
     2161 ttcaccaaat tacccagttt cttctttgtg aattgttccc actcttctgc tggtatatac
     2221 tcatctacat ttaacagatt gatatgtttc gatgttgttc tactgtattt ttcttgtgtg
     2281 gcatatattc taccatctac ttcaaaggca acaagggttt catatgaaaa atatagaact
     2341 gtttcaccga tatttatcct tgtgaaattg ggcgctatct gttttaatct tactatggga
     2401 aatccaccct ctgttaaaat ttgatttctt attaattctc ccatattact tctcctcttc
     2461 tttctttttt acaccgaatt tcttcctcaa aagctctttg gcatcctcaa gagtgcgtct
     2521 tctagcactt gaaagcttat cacccgccct gttaatataa aaattaagcc gtgacatggc
     2581 ctgtttcaag tcattgctct gtgaatgaag aaccttcgct atattctctg cacttgaagt
     2641 aaaaaggccc tccggtggtt cccattttgt tgccacttca cccgaccata gtttctcatt
     2701 gatgagataa aggtcaattc tttctaaaag atctcgtttc atctccatat actgatcttc
     2761 ataatcccta tcatttggat atttttttct gagtaatttt agttcagata ttctatcgtt
     2821 atatctgtca agtctttgtt gtaaatcgga tttttgcata tctactctca attgctgaaa
     2881 ggtttgaaca gtctgttgtt ccatttttgc aagttcttta cttgtagcat atctaccatc
     2941 cagcgcaaag atagaactga caagaccaaa aacaattccc attgttataa gtatattttt
     3001 caagttaatt tctggcatac aatatactcc tatactttat caatagtatt tataatttat
     3061 ttattggttt tcttataaat aacttatgaa gaacatatat tataaatcaa ggagtgtgaa
     3121 aatggttgaa atgtcttttt ttgacaaggt aaagtatctt gatttaataa tagattgttt
     3181 acctgacgca atctttgcta ttgatctaca gggaaatgtt attgcttgga acaaacagat
     3241 ggagaaactg actggttgta aaagagatac cgtcattgga aaaggagacc atctgtattc
     3301 aatcccattt tacggtaaaa agacaaaaat cctcatcgac cttgtattcg aacccgatcc
     3361 ggaattggaa agaagatatg attattttga aagattgaac gaaaatgtcc taacctgtca
     3421 catctatctt gaaaagattg gaaaaaccct ctgggcaaaa gccacccctt tgaaggatga
     3481 aaataatgag attattggtg caatagaatc catcagagat atttcagaaa atacaatact
     3541 tctcaagaaa ctgtcattga gtgacgaaaa attccagaat ttatacaata tcactcaatt
     3601 attccttgac agtatcaatg atatggtatg ggccaaggat atagataaca gattcatatt
     3661 tgcaaacaaa tccttcgaaa aatttctcaa gaaaaaatca attgatttaa ttggaagaaa
     3721 atcatttgat atatatggac agggaaaaaa gttcgaagag gccgatgaaa tagcaaaaac
     3781 aaaaccacat catatcatag aatcaattaa tattgacgga gaagaaaaat ggttggatat
     3841 atgcaaggca ccccttcttg acagaaaagg aatgctcctc ggaacaatag gaacagcaag
     3901 agacataaca agagaagtag aattggtaaa acagagaaat atagaaattg ataatctaaa
     3961 aaagataatt gaagagaaag taaaagattg gagaaaagaa aatgaagaaa tccacagaga
     4021 tatttcaaag aaagtatacg aatcaataat gttattgaga ggtatacaaa atgatgccaa
     4081 gcaaaataat attgaagaac taaataaaga ggagatattt ttctaatgcc tgacgaaaga
     4141 ttagttccag aatccatagt caaacttcta tttgaccaaa taaaatccgc agcagatgca
     4201 aacaccactt ctgtcaagga attgggagaa gctgtgtttg aagttctaag ggcgcagggt
     4261 aatctcgcga ccaaaaaaga cataatagat gaacataatc ttgtagatca gaaaaatagt
     4321 gaaaaaataa aatatattat acagaaaata gagaaaatgg aagagattgt tggtggtaaa
     4381 ataaatggtg ttggaaaaac tcttgaagaa gaagaaaata tgataaataa gataggaagt
     4441 atagaaacta aggtggataa catagagtca aaaataaaaa caatgataac agttgttatt
     4501 gttgcctttt ctgttctttt ggcatccttc tattttgtaa agagtgccac ggatgtggca
     4561 gttcaaagtg ctatggacag agcggtaaaa gaaatcatta caacaagacc gtccaacata
     4621 tatcctccgc caagtaaata agagggagaa atgagattat taaattttct aaaaaagatt
     4681 gaggaagaga acggaggggg agcaggaatg ggtggtgcaa ccacaacagc caatatcgcc
     4741 actttcgctc aaaaaattcc aggtgggcct acacgaagag gtgctttaaa aaattggaaa
     4801 aaatcaaaaa agaagagaat gtttcctata tgaaactcat ccaatatcta atagagcaga
     4861 acatagataa aataaaaata gtcattatca ccggcagaaa aaatggaaag gatagtaatg
     4921 atttatatag aaccgctaaa agattcaagg aagcctgtga cgaaagaaaa atagagtgtt
     4981 atgttgcctt ttcggatgat ggttatatag caaggaaaaa cggcaaagtt tatatacaca
     5041 atatagatga tgaaaatgga tttgaaataa actcatcaaa taccgtggca atcatcaggg
     5101 gaactacagg taaaagaaaa tccagccttg acctcatatc acaactcgaa agacataata
     5161 ttttctgtgt aaatagcagg cgtactatgg aggagtgttc tgacaaatat agaacagctc
     5221 tcattatggc ggatgccaaa ataccaacac caaaaacagc tattattttg ggccctgatg
     5281 gtattgatgt tgcacttaaa aagataggga aaaaatttcc tgttgtcctc aaaacattaa
     5341 ctggttcaaa aggtattggt gtattcgttg caga
//
LOCUS       DAOTQL010000116         5343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_12321_length_5343_cov_5.343986, whole genome shotgun sequence.
ACCESSION   DAOTQL010000116 DAOTQL010000000
VERSION     DAOTQL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12321_length_5343_cov_5.343986"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="P5293_06905"
     CDS             complement(<1..1735)
                     /locus_tag="P5293_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HRR49663.1"
                     /translation="MFKNIYYNTKTSTIHLWEQLKGENFYTEIPWVPYVFYPYEKGDV
                     KTIEGQPVTKRLFRSYSDYFGYLKGKENLFEDNVRPEIQFLTERYYGIPESEIEVPKL
                     KTYFFDMEVDNKVGFPHPEDARDPICLVSIYDNSIDTTTVFGLRDYKNGKYSKEKYVR
                     YIKCDNEKVLLTRVLNFFHKYPCDVISGWSISNFDIPYLINRTKRVFEDSKMFMRLSP
                     INDVQVWEAKSGAMNVNISGITILDYIDLYKWYSPVKLERYSLDFVSKYELEKGKIDY
                     SQYKDIRELCEKDWDLFVDYNITDALRVFQLDKKLNYIGQVQALSLLTKCPMKYYNVM
                     TQLIEGLLLTHYRRNGLCAPKFVGGHQEPFMAAYVKEPQQGKYDWVVDLDIASSYPTA
                     IIILNMSPETYYGRILDMTEDIIIQSVRQRKFSDFNLLKDGKKIAFTGKKLEIFNEAI
                     EKKLISIAPCGSVFSTKPIGVLPQIEKDVFQRRVEIKNNMIKMKKSLSELRDENKKKI
                     EERINQFNSLQNALKILLNAVFGVTSVPYSRYFNVNISEAITSCGRQTIKAGERYVND
                     LLNNPDKNDELR"
     gene            complement(1735..2157)
                     /locus_tag="P5293_06910"
     CDS             complement(1735..2157)
                     /locus_tag="P5293_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49664.1"
                     /translation="MVRKKDNIKVMLNGEFFVLSGDLKGALSSVLSVWYSKINYFSAE
                     NMRDKIEGAISLVEELMDTKSEARTKRQITNIRKYLERDLTREQLIKFYTDIIMAGEG
                     LSTLSGFGIAKTSTTAGKRSGKAAAGLNAEKLSLYEMK"
     gene            complement(2163..3479)
                     /locus_tag="P5293_06915"
     CDS             complement(2163..3479)
                     /locus_tag="P5293_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRR49665.1"
                     /translation="MLENFFDVKEDKIEDCNCLPLYTTLEFPEELNLLWPAVRDMSEE
                     EFERYAEKLRIFFLEKYEKDRYPIGAGSASLDDIITRLKKFHNLNINHPDVFFEEDGQ
                     KFLKGYNAWSTVIDHWFPEMMDVPITRGINQLTPSIIDLFRDKNVYMKKMKRMLWKDK
                     LNGWRDNPYKQVWPTLKQSIRMGSGTQPVSNIRCPVAKWIYQTYMLKLLPEVEGDEVV
                     VFDPSMGWGGRLISFLSASSKIRNKKCIYIGTDPNSVIYERYGMVERFWKKFIDPSCT
                     AEVHPLCMGSEEFDKSDLFKKYKGKAVLAYTSPPYFNRERYSTDEQQSYKKFSSYDSW
                     KNGFLKKTMENVRDFLMPNGIFVWNIANIKLSKGKYLKMEDDSVAIAKQVGMKEYDKI
                     HMLMRFTIGRDNNLEGIAERASMNVIKCEGKLNKYEPVFVFRKEKG"
     gene            complement(3525..3755)
                     /locus_tag="P5293_06920"
     CDS             complement(3525..3755)
                     /locus_tag="P5293_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49666.1"
                     /translation="MRPGPRRSGSSPFVGKAGKILWDRIYVCDCGNIIDRDLNASINL
                     EKYGLIKLNDTELQDAFGQEAVSVKQEENIKC"
     gene            complement(3878..4087)
                     /locus_tag="P5293_06925"
     CDS             complement(3878..4087)
                     /locus_tag="P5293_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49667.1"
                     /translation="MRKRWWERKERRSCGMEWFVLLTIFLISLKVLGFIRWSWWWIFA
                     PIWLPPLIGIIFFLICMGFIWLLDG"
     gene            complement(4084..5235)
                     /locus_tag="P5293_06930"
     CDS             complement(4084..5235)
                     /locus_tag="P5293_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRR49668.1"
                     /translation="MLLDNISKNYYHVDEYKVYTDIGFIDIDYIIETIPFRKMLVLFE
                     SGIEIECADNHVFITSDDEEIFARDVFSGCRIKGIDCIETVFDVFDTGENVEMYDISL
                     PHHHKFYTGGVLSHNSNTLANIGARQVLHGHNVFLCTLEMSEDAFAQRFDSIFSKLDI
                     NKMYTLESMKGQLISRLRKIKSETPDRGKLIIKQFPTGEASVQDIKRLIREWIIRGIK
                     PDIIYLDYINIMKPTYSSKDNLYSDVKRIAEECRAMSFQFECPVVSVSQLNREGSMIA
                     FNEVDFVYIAESMGVPATADFMAIYGIDDEKLIYESELHYKIVKNRIGGRVGEVDKFY
                     YDSRSLKMYDSSELDMWLEDAKETGDERNVVQITQEYRGQRSRLSRGRR"
BASE COUNT         1622 a         1170 c          754 g         1797 t
ORIGIN      
        1 ctcttaattc gtcattttta tctggattgt tcaataaatc gtttacatat ctctctccag
       61 ctttgattgt ttgtctaccg caggatgtaa tggcctcaga tatgtttaca ttgaaatatc
      121 gtgaataagg aacactcgta acaccgaaaa cagcattgag aagaattttg agagcgttct
      181 gtaaagaatt gaattgattt attctctctt ctatcttctt cttgttttca tccctcaatt
      241 ccgataaaga ttttttcatc tttatcatat tatttttaat ctctactctc ctctgaaaga
      301 catctttctc tatttgtgga agaacaccta tcggtttagt ggaaaagaca gaaccacaag
      361 gagctatact tataagtttc ttctctattg cctcattaaa tatttcaagt ttcttaccgg
      421 taaaggcaat tttcttccca tcttttagaa gattaaaatc tgaaaatttc ctctgtctga
      481 cagactgtat aataatatcc tccgtcatat caaggatacg accataataa gtctccggtg
      541 acatattcaa tataattatt gcggtgggat atgacgaagc aatatcaagg tcaacaaccc
      601 aatcatattt tccctgttgc ggttccttga cataggctgc cataaatggt tcttgatgcc
      661 caccgacaaa tttcggtgcg cacaatccat ttctcctgta atgtgtcaat aacaaacctt
      721 caatcagctg tgtcattaca ttataatatt tcattgggca ttttgtgaga agcgagagag
      781 cctgaacctg gccgatatag tttagtttct tatccagctg aaataccctc aaggcatccg
      841 taatattata atcaacaaat aaatcccaat ctttctcaca taattccctg atatccttgt
      901 actgagaata atcaatcttg cccttttcaa gttcatactt agaaacaaag tcaagagaat
      961 atctctccaa ttttacgggc gaataccatt tatacaaatc aatataatcc agtatagtta
     1021 taccggatat attcacattc attgctccag actttgcctc ccatacctga acatcattga
     1081 tgggagacaa tctcataaac atcttcgaat cttcaaatac tctcttggtt ctgtttataa
     1141 gatatggaat atcgaagttg gatatcgacc atccagaaat aacatcacag ggatacttat
     1201 gaaagaagtt gagaaccctt gttagtagaa ctttctcatt atcacattta atatatctga
     1261 catatttttc cttcgaatat ttcccgtttt tataatccct caaaccgaat acagtagttg
     1321 tatcaatcga gttatcataa attgatacga gacagatggg atcacgggca tcttctggat
     1381 gtggaaaccc aactttgtta tctacttcca tatcaaagaa atatgttttc agtttaggaa
     1441 cttcaatttc actttcaggt attccataat atctctctgt aagaaactgg atttcaggcc
     1501 ttacattatc ctcaaataga ttttcctttc ctttcagata accaaaataa tctgaataag
     1561 acctgaatag ccttttagtg actggctggc cctctatagt ttttacatcg cccttttcat
     1621 acggatagaa aacataggga acccaaggaa tttccgtata aaaattttca cctttgagtt
     1681 gttcccaaag gtgaattgtt gatgtttttg tattatagta tatgttcttg aacattattt
     1741 catctcatat agagataatt tctctgcatt taacccggcg gccgccttcc cacttctttt
     1801 acccgccgta gtagatgttt ttgctatacc aaaaccggaa agtgttgaaa gaccctctcc
     1861 ggccataatt atatcagtat aaaattttat caattgctct ctggttaaat ccctttcaag
     1921 atatttcctg atatttgtaa tttgtctttt tgttctggct tcagattttg tatccatcaa
     1981 ctcttcaaca agagaaatcg cgccctctat tttatccctc atgttttccg ctgaaaaata
     2041 atttattttg gaataccaga cagataaaac tgaactcagc gcccccttga gatcaccact
     2101 taagacaaaa aattcaccgt tcaacatcac ttttatatta tccttttttc tcaccataat
     2161 tgttatcctt tctctttccg aaagacgaat acaggttcat atttgttcaa tttgccttca
     2221 cactttataa cattcataga ggccctttct gcaatacctt caaggttatt atccctacca
     2281 atcgtaaatc tcatcaacat atgaatttta tcatactctt tcataccgac ctgtttagca
     2341 atcgcgacag aatcatcttc catttttaga tatttacctt tggataattt tatattggct
     2401 atgttccata caaatattcc attaggcatt aaaaagtctc ttacgttctc cattgtcttt
     2461 ttcaaaaaac cgtttttcca agaatcataa gaagaaaatt tcttatagga ttgctgttca
     2521 tcggtactat atctctcacg attaaagtaa ggtggtgatg tatatgccaa aactgcttta
     2581 cctttatatt tcttgaacag atctgacttg tcaaactcct ctgatcccat acaaagcgga
     2641 tgtacttccg ctgtacagga tggatcgata aattttttcc agaacctttc aaccattcca
     2701 tatctctcat agatgactga atttgggtct gttccaatat agatacattt cttgtttctt
     2761 atcttcgatg aggctgaaag aaaggaaatc aaccttccac cccatcccat tgaggggtca
     2821 aatacaacaa cttcatcgcc ctcgacctcc ggaagaagtt ttagcatata agtctgatat
     2881 atccatttcg caacagggca tctaatattg gaaactggct gtgtccctga tcccatccgt
     2941 attgattgct tgagagttgg ccatacctgt ttatatggat tgtctctcca tccattcaat
     3001 ttatccttcc acaacattct cttcatcttt ttcatataaa cattcttgtc cctgaagaga
     3061 tcaattatag atggtgtcaa ttgatttatt ccacgagtaa tgggaacatc catcatttcc
     3121 ggaaaccaat ggtcaataac agttgaccag gcattgtagc ccttcaaaaa tttctggcca
     3181 tcttcttcaa aaaagacatc gggatgattt atattcaaat tatgaaattt tttcaacctt
     3241 gtaattatat cgtccaaaga ggcggatcca gcaccaatag gatacctgtc tttctcatat
     3301 ttctccaaaa agaaaatgcg cagtttttca gcatatctct caaattcctc ttcggacata
     3361 tccctcacag ctggccacag cagatttaat tcctctggaa attcaagagt agtataaaga
     3421 ggaaggcaat tacaatcctc tattttatct tctttcacat caaaaaagtt ttctagcatc
     3481 accaaatttt tctatactct ccgttctgaa aaacttactc aaatttaaca tttgatgttt
     3541 tcttcctgct tcactgaaac tgcttcttga ccaaaagcat cttgaagttc ggtatcgttg
     3601 agtttaatta atccatactt ctcaagatta atagaagcat ttaagtctct gtctatgata
     3661 tttccacaat cacatacata aatcctatcc cataaaatct taccggcttt accgacaaaa
     3721 ggtgagctcc cggatctcct cggcccgggc ctcacctatt atcgcataac ggaaagattc
     3781 tgaccagtag ggcttcgctc taccgttctg aaagagaaaa catatttcac atttactcac
     3841 tacaccgtca aataaatgta acattcgtaa ttgtttctta tccatcaaga agccaaataa
     3901 aacccataca gattaaaaag aatattattc ctatcaatgg gggcaaccaa attggtgcga
     3961 atatccacca ccaagaccat ctgataaaac caagaacttt gagagatatc agaaatattg
     4021 tcaataaaac aaaccattcc ataccgcaac ttctcctttc ttttctttcc caccacctct
     4081 ttctcatctt ctgcctcttg acagtctact tctctggccc cgatattcct gtgtaatctg
     4141 aacaacattt ctctcgtcac cagtttcctt agcatcttct aaccacatat caagctcgga
     4201 tgaatcatac atcttcaagg aacgggaatc ataataaaac ttatccactt caccaactct
     4261 accaccaatt ctgttcttca ctatcttata atgcaattca gattcataaa tcaatttctc
     4321 atcatcaata ccgtatatcg ccataaaatc tgctgtggcg ggtactccca tactttctgc
     4381 aatatacaca aaatcgactt cattgaaggc tatcatcgag ccttctctat tcaactgtga
     4441 aacggaaaca actggacact caaactgaaa agacattgct ctacattcct ctgcaattct
     4501 ctttacatcg gaatacaaat tgtccttact tgaataggtg ggtttcatta tattgatgta
     4561 gtcaagatag atgatatcgg gttttattcc cctaataatc cactctcgta tcaatctctt
     4621 tatatcctga acagaggctt ctccggtggg aaattgttta ataataagct tccctctatc
     4681 cggtgtctca ctcttgatct ttcttaatct ggaaatcaat tggcccttca ttgactcaag
     4741 ggtatacatc ttgtttatat cgagtttact aaagatactg tcaaatctct gagcaaaagc
     4801 atcctccgac atttcaagag tacagaggaa aacattgtgg ccgtggagaa cttgtcttgc
     4861 tccgatattg gcaagtgtgt tggaattgtg tgataaaaca ccccctgtat aaaatttatg
     4921 atgatggggt aaagaaatgt catacatttc cacattttct ccggtatcaa aaacatcaaa
     4981 aactgtttcg atacagtcga tcccctttat tctacatcca gaaaaaacat ctctcgcaaa
     5041 tatttcttca tcatcggatg taataaaaac atgattatct gcacattcaa tttcaattcc
     5101 agactcaaac aaaacaagca ttttacgaaa aggaattgtt tctatgatat aatcaatatc
     5161 aataaatcca atatcagtat aaaccttata ttcgtcaaca tgataataat tttttgatat
     5221 attatccaat aacatttttt aattcccgaa taattttatt tttatctgtt ttccaatcac
     5281 ttatacaatt tgacactccc cgtgactaaa gttaggggat tctttggtag tagtcggaag
     5341 tcc
//
LOCUS       DAOTQL010000117         5280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_12500_length_5280_cov_4.974354, whole genome shotgun sequence.
ACCESSION   DAOTQL010000117 DAOTQL010000000
VERSION     DAOTQL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12500_length_5280_cov_4.974354"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="P5293_06935"
     CDS             complement(<1..471)
                     /locus_tag="P5293_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HRR49669.1"
                     /translation="MSTLEIVGLAIGLCFDTFAISLSSGICLPQIKRGEFLKIVFTFA
                     LIQSLFMVAGWLLGLGLENLIKTYDHWIAFAVLLFIGGKMIWDAFFKKGDGECIDIRR
                     FRIMIIAASATSIDALAVGVTLAIIDIIISEFLFAIVAIFITTALASTVGLKFGR"
     gene            complement(471..1979)
                     /locus_tag="P5293_06940"
     CDS             complement(471..1979)
                     /locus_tag="P5293_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRR49670.1"
                     /translation="MHQFPVSWLLIAFTLYTIMLFAVSWYTSRKADTGAFFSGNKKSP
                     WYIVAYGMIGTSISGVTFISVPGNVLNQNFYYMPLVLGFVVGYIIIAKVLIPLYYKMN
                     LTSIYTYLEHRFGFFTYKTGASVFMVSRVLGAAVRIFVVVLVLHTFLPAGSVPFWVVA
                     FIFMLLIFLYTFKGGVKTIIWTDVLQTTFMLLAVFLTIFSISRAMNWHFGDMVSAVAS
                     TDYSRWFDWEWSHGTNAIKQFISGIFITVAMTGLDQEMMQKNLSCKNIKEAQKNVYTT
                     SITIVIVNFFFLLMGALLAIFVDSKLGGMHSIGLADASGVFTKAQTDKLFPVIASQYL
                     GLAVGLFFIIGLISASYPSAGGALTSLTISFCIDFMGFNKRKDLTEKKKQHIRQIAHG
                     VFALLFFIIIIILYMVNNQAVIDLVYLLASYTYGPLLGFFFFGLLTKYQVKDKYMPYV
                     AIGSPLLCYLLDVAGKSLLGFGFGFTILIINGLITFTAMYLLRERGIKVAKV"
     gene            complement(1992..>2181)
                     /locus_tag="P5293_06945"
     CDS             complement(1992..>2181)
                     /locus_tag="P5293_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HRR49671.1"
                     /translation="IVLSTTMEGETTSFYIYKQLSKFNIKITTIARGIGFGDDLEYTD
                     EVTLGRSIQNRQPFVPLK"
     assembly_gap    2182..2191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2192..2623)
                     /gene="recR"
                     /locus_tag="P5293_06950"
     CDS             complement(<2192..2623)
                     /gene="recR"
                     /locus_tag="P5293_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRR49672.1"
                     /translation="MLYHNYPSRLLQDAVEELSSLPGVGRKSALRFAMHLLKQPKENV
                     ERFGQSIIRLSKESKFCRDCFMISDYDVCEICADSRRDHSIICVVESIRDVISIENTM
                     QYNGIYHVLGGIISPMDGVGPSDLTIEQLLRRVEDKGIEEVL"
     gene            complement(2624..3445)
                     /gene="murQ"
                     /locus_tag="P5293_06955"
     CDS             complement(2624..3445)
                     /gene="murQ"
                     /locus_tag="P5293_06955"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRR49673.1"
                     /translation="MKITELPSNYSDLEKMSTRELLENINREDKTVPYAVEKCIPQIE
                     KLVENIVPRIQNGGRVFYLGAGTSGRLGILDASEIPPTFGAPYDLMIGLIAGGDKAIR
                     KAVEAAEDSFEGGWQDMQQYGVDQDDVVIGIAASGTTPYVLGAVIEAKKRGILTACVT
                     CNPDSPLATEVDIPIVAVVGPEFVTGSTRMKSGTAQKLILNMISTVSMIKLGHIKGNK
                     MVDMQLTNAKLIDRGTRMIMEEIESNDYEFAKSLLLKHGSVRKAVDFYNSSKGLA"
     gene            complement(3458..4297)
                     /locus_tag="P5293_06960"
     CDS             complement(3458..4297)
                     /locus_tag="P5293_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRR49674.1"
                     /translation="MILIADSGSTKIDWRAVGSDRSVKGFTTPGINPYYQTDDEIIEE
                     FKENLLPNIPSNVKVSEVNFYGAGVTPEKTGNLSICFKALFDDVKVFASTDLLAAARA
                     LCGHEPGIAAILGTGGNSCFYDGTKIVDNVPACGFILGDEGSGAVIGKNFISDYLKRQ
                     IPQDLRACFDERFGLDYGAIVEKVYKQPYPNRFLASFSPFVHENRDNPYVKRLLNKCF
                     GDFITRNILQYDYKNYSLNIVGSVAFYYQDEIREVASEYGVILGKILKSPIEGLVNYH
                     LNM"
     gene            complement(4374..4949)
                     /gene="pnuC"
                     /locus_tag="P5293_06965"
     CDS             complement(4374..4949)
                     /gene="pnuC"
                     /locus_tag="P5293_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRR49675.1"
                     /translation="MDQNIVIELFGAITGLVYVYLEIVQRRTMWIVGGVSALIYIVVF
                     FSSGLIAASFLQLCFFGMSVYGWIAWSRADDKNAPKVVKLKRNKLVTSIICSLIFYVI
                     ISFSLVKFSSDPMPYIDSLITVLSLLATYWVIGRHSENWIVWIINNAIAIYLYASQAL
                     YPTVLLYIVYLISSCIGYLHWLKLSAVSVKN"
     gene            complement(4955..>5280)
                     /locus_tag="P5293_06970"
     CDS             complement(4955..>5280)
                     /locus_tag="P5293_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49676.1"
                     /translation="GTLVNKNGYLLALSSKYVGKQYYDNTADDTRSIPSYCTAALILG
                     KSFHIGLQSSIDINFYVDNLFNKMYFSNAWIYMAEFADGGKYVEEGFFPQPGRNYSLK
                     ATFRF"
BASE COUNT         1790 a         1116 c          857 g         1507 t
ORIGIN      
        1 cctaccgaat ttcaacccca ctgtagaagc aagagccgtt gtaataaata tagcaacaat
       61 agcaaaaaga aattcagaaa taattatgtc aataatagcc agagtaactc caacggctag
      121 ggcatcaatg ctggtagctg aagctgcaat tatcattatc ctgaatctcc ttatatctat
      181 acactccccg tcaccttttt tgaaaaaggc atcccaaatc atttttcctc ctataaaaag
      241 taaaactgca aatgcaatcc aatgatcata tgtcttgata agattctcca aaccaaggcc
      301 taataaccat cctgcgacca tgaagagaga ctgaattaat gcaaaagtaa atacaatttt
      361 aagaaactct ccacgtttta tctgaggcag gcaaattccc gaagaaagcg agattgcaaa
      421 cgtgtcgaag cataagccaa tagcaagtcc aacaatctca agagttgaca ttatacctta
      481 gctaccttta ttcctctttc cctcaagagg tacatggcag taaatgttat aagtccatta
      541 attataagta tcgtaaatcc aaatccaaat ccgagaagac ttttgccagc cacatccaga
      601 agataacaga gcaacggcga accaatagct acgtaaggca tatatttgtc tttaacctga
      661 tattttgtta gaagtccaaa aaagaaaaat ccaagaagag gaccataagt atatgaagcc
      721 agcaaataaa caagatctat aaccgcttga ttattaacca tatataatat tataataatt
      781 ataaaaaaga gaagagcaaa tactccatga gcaatttgtc tgatatgttg tttcttcttc
      841 tcggtaagat ctttccgctt gttaaatccc ataaaatcga tgcaaaaaga gattgtaagg
      901 gatgtaagag caccacctgc acttggatag gaggctgaaa tcaaaccaat aataaagaaa
      961 agtcccaccg caagtccaag atattgactc gctataacag gaaaaagttt gtctgtctgt
     1021 gctttagtaa atacacctga tgcatctgca aggccaatac tgtgcatgcc tccaagctta
     1081 gagtcaacaa aaatagcaag aagagctccc atcaaaagga agaaaaaatt tacaattaca
     1141 attgttatgc tcgttgtata gacatttttt tgagcttctt ttatattctt gcacgaaaga
     1201 ttcttttgca tcatctcttg atcaagccct gtcatggcga cagtgataaa tattccgctg
     1261 atgaactgtt ttattgcatt tgtaccatgc gaccactccc agtcaaacca tctagaatag
     1321 tctgtagatg caactgccga taccatatcg ccaaaatgcc aattcattgc cctcgaaata
     1381 gaaaatatag taagaaaaac agcgagaagc ataaaggtag tctgcaatac atctgtccaa
     1441 ataatagtct taacacctcc cttaaaagtg tacaggaata taagcaacat gaaaatgaag
     1501 gcaactaccc aaaacgggac agaacctgca ggcaaaaagg tatgaagcac caagaccaca
     1561 acaaatattc tcacagctgc accaagcact ctcgacacca taaataccga agcccctgtc
     1621 ttataagtaa aaaaaccaaa acgatgttcg agataggtat atattgaagt taaattcatc
     1681 ttgtagtaga gaggaataag tactttggca ataattatgt aacccaccac aaagcccaag
     1741 acaagaggca tataataaaa attctgatta agcacattcc cgggaacaga gatgaacgta
     1801 actcccgaaa ttgatgttcc tatcattccg taagctacta tataccacgg agattttttg
     1861 ttcccagaaa aaaatgctcc tgtatctgct ttcctcgatg tataccaaga aacagcaaaa
     1921 agcattattg tgtaaagtgt gaaggcaatt aaaagccaag ataccggaaa ttgatgcata
     1981 ataattaatt tttattttaa tggtacaaaa ggttgtctgt tttgaataga tctgccaagg
     2041 gtaacctcat cggtatactc caaatcatca ccaaacccta ttcccctggc gatagtagta
     2101 atcttaatat taaacttgct gagttgctta tatatgtaaa aagaagttgt ctctccctcc
     2161 attgtagtac taagcacgat tnnnnnnnnn ntagcacttc ttctatacct ttatcctcca
     2221 ctcttcttaa aagttgttct attgtcaaat cagaaggacc gactccgtcc attggagaaa
     2281 ttattcctcc aagtacgtga tatataccat tatattgcat ggtattctca atacttatga
     2341 catctctgat actttctacc acacaaataa ttgagtgatc ccttctactg tccgcacaaa
     2401 tttcacaaac atcataatca gaaatcataa agcaatccct gcaaaatttg gattccttac
     2461 tgagacgaat aatagattgg ccaaatctct cgacattttc tttgggctgt ttaagaagat
     2521 gcatggcaaa acgcaaagca cttttacgcc caacacctgg aagtgaggat aactcttcca
     2581 cagcatcttg aagcagtctt gaaggataat tatgatataa catctatgcc agaccttttg
     2641 acgaattgta aaagtcgaca gccttcctca cagaaccatg ctttagaaga agcgatttag
     2701 caaattcgta atcatttgat tcaatctctt ccataatcat cctggtgcct ctgtctatga
     2761 gttttgcatt tgtaagctgc atgtctacca ttttgttacc ctttatgtga ccgagtttta
     2821 tcatggaaac agttgaaatc atgtttaaaa tcagtttttg agctgtaccc gatttcatac
     2881 gtgtacttcc agtgacaaat tcaggaccta ccacagccac aatagggata tcgacttcag
     2941 tagccagtgg agaatcggga ttacatgtta cacatgctgt gagtattcct ctttttttag
     3001 cctctatcac agcacctaag acataaggag tagtgcctga ggcagcgata ccaataacta
     3061 catcgtcctg gtctacacca tattgctgca tatcttgcca accaccctca aaactgtcct
     3121 ctgccgcttc taccgccttt ctgatagctt tgtctccacc tgctataagc cctatcatga
     3181 gatcataagg ggccccaaaa gttggtggaa tctcagaagc atcaagtatg ccaagtcttc
     3241 cgcttgttcc tgctcccaaa taaaaaacac gacctccatt ttgaatgcgt ggaacaatat
     3301 tctcaaccaa tttctcaatc tgaggaatac acttttcaac agcatacggc acagttttat
     3361 cttctcgatt aatgttttca agaagttctc ttgtagacat tttttcaagg tcactgtaat
     3421 tcgatggcaa ttctgttatc ttcatatttt aaattaatta catattcaag tggtaattaa
     3481 caagcccctc tatgggagat ttgagaatct tgccaagtat aactccatat tccgaagcaa
     3541 cttctctgat ttcatcctga taataaaagg ctacagaccc tacaatgtta agagaataat
     3601 ttttatagtc atactgaaga atattacggg taatgaaatc accgaaacat ttattcaata
     3661 atctcttgac ataagggtta tctctatttt catgaacaaa tggggagaat gaagcaagaa
     3721 atctgtttgg ataaggctgt ttataaactt tttccacaat tgctccataa tcgagaccga
     3781 aacgttcatc aaagcatgcc ctcaggtctt gaggtatctg ccttttaaga taatctgaga
     3841 taaagttttt gccaataacg gcaccgctgc cttcatcacc aagaataaac ccacatgcag
     3901 gaacattatc cacgattttg gttccatcat aaaaacagga attacctcct gtaccgagaa
     3961 tggcagcaat ccccggttca tgcccacaaa gagcccttgc agcagcaaga agatctgtag
     4021 atgcaaagac ctttacatca tcaaaaagcg ctttaaaaca aatgcttaga ttacctgtct
     4081 tttcgggagt aacccctgca ccataaaaat tcacctcact tactttaacg tttgatggta
     4141 tattaggaag aagattttcc ttaaattctt caattatttc atcatctgtc tgataataag
     4201 gattaattcc gggtgtggta aagcctttga cactcctgtc acttccaaca gctctccagt
     4261 caatctttgt tgagccactg tctgcaatta aaatcatgtc gtcttttatt attgaaattt
     4321 atacaatagc ctggcaaaag tacgtttttt gatattttat tgcaaacatc aacctaattt
     4381 ttcacagaaa ctgctgatag ttttagccaa tgcaggtaac caatacaaga tgagatcaaa
     4441 tagacaatat atagaagcac tgttggataa agagcctgac ttgcatagag ataaattgca
     4501 atagcgttgt ttattatcca cacgatccaa ttttcagaat gacgccctat tacccaatag
     4561 gtagccaaca ggctcagaac agttatcaaa gagtcaatgt acggcattgg gtctgaactg
     4621 aatttaacca gtgaaaatga aataatcaca taaaaaatta aggagcagat aattgaagta
     4681 actaatttat ttcttttcaa ctttactact ttcggagcat tcttgtcatc agctctactc
     4741 catgcaatcc agccgtaaac actcatacca aaaaaacata gctggagaaa ggaggcagca
     4801 ataagacccg atgaaaaaaa tacaactatg tagatcaggg cggatacacc gcccacaatc
     4861 cacatagttc tccgttgtac aatctcaaga tagacataaa ccaatcctgt gatagcacca
     4921 aatagctcaa tgacaatatt ctgatccatt aaatctaaaa tctaaaagtg gcctttaatg
     4981 aataatttct acctggttga gggaaaaaac cctcttcgac atatttccct ccatctgcaa
     5041 attctgccat atatatccag gcattagaaa aatacatttt attgaaaaga ttgtctacat
     5101 aaaaatttat gtctatagaa gactgcaatc ctatatgaaa acttttgcct aaaatcaaag
     5161 ctgcagtaca atatgaaggg atagatctcg tatcatctgc ggtgttgtcg taatattgct
     5221 tccctacata ctttgaagaa agagcaagaa gatatccgtt tttatttact aaagttcccc
//
LOCUS       DAOTQL010000118         5057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_13162_length_5057_cov_4.682127, whole genome shotgun sequence.
ACCESSION   DAOTQL010000118 DAOTQL010000000
VERSION     DAOTQL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13162_length_5057_cov_4.682127"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..243)
                     /locus_tag="P5293_06975"
     CDS             complement(100..243)
                     /locus_tag="P5293_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49677.1"
                     /translation="MKQDSEELMLTKAIIDGDKSSFNGRESFSVAVLRSSRARPLTRR
                     QGA"
     gene            326..>1575
                     /gene="aspS"
                     /locus_tag="P5293_06980"
     CDS             326..>1575
                     /gene="aspS"
                     /locus_tag="P5293_06980"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRR49678.1"
                     /translation="MYRTHTCGELRINDAGKQVMLAGWVQRIRKMGGMSFIDLRDRYG
                     ITQLTVDESDSKELNAVAEKLGREYVIQATGEVAERTNKNPKISTGDIEVKLSAIKVL
                     NPSLIPPFTIEDESDGGEELRAKFRYLDLRRNPLRKALELRHKLAQETRRYLDEHGFM
                     EIETPFLIKSTPEGARDFVVPSRMNPGEFYALPQSPQQQKQLLMIAGFDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMSFVEREDILCMFEGMIKSLFKNVLGRDFNYSFPRISYS
                     EAMDEYGSDKPDIRFEMKIKEITNIVQGKGFSVFDAAEYIGAIVVNNCVSYTRKQLDE
                     LTEWVKRPQIGAKGLVYARYTEDGIKSSVDKFFSPEELSSLCKVIDAKVGDLVLILCG
                     NRKKTQEALGTLRIEMGNRLGLRQ"
     assembly_gap    1576..1675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1676..1936
                     /locus_tag="P5293_06985"
     CDS             <1676..1936
                     /locus_tag="P5293_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRR49679.1"
                     /translation="LGYSAEEVEYKFGFMINAFKYGAPPHGGIAFGFDRLCALFGGQE
                     SIRDFIAFPKNNSGRDVMLDAPSRIDKVQLDELFLRSTFEGN"
     gene            1964..3004
                     /locus_tag="P5293_06990"
     CDS             1964..3004
                     /locus_tag="P5293_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRR49680.1"
                     /translation="MLIFYLLLALSVSFLCSIMETVLLTASVSFLNMKVNQGIKAASI
                     LRDQKQKIDRPIAAILSLNTVAHTVGASGVGAQATALFGDAYFGIISAILTLLILVLS
                     EILPKSVGAHYWKHLVVPAGYIIRCMIVIAYPLVILSEQMTRLISRGSKDEATVSREE
                     VAAMVDIGVEEGTFEDTEKKIIQNLIKLRSVRASDVMTPRVVVATASEEITIGEFYKN
                     KTYLHYSRIPVYSDNDKEKICGFVYRQEVLEHLANDNFDLCLKEIKKPIMIAPTSQPL
                     TIMWERLTSQKTHIAMIVDEYGGFEGIVTLEDIIETIMGIEIMDDRDIVADMQQFARE
                     RWEESVKKSTNT"
     gene            complement(3012..4070)
                     /locus_tag="P5293_06995"
     CDS             complement(3012..4070)
                     /locus_tag="P5293_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRR49681.1"
                     /translation="MTQKEIEDVLRGVVHPAKDKDIVSLEMVEDIKVEDGKIRFKLVF
                     SSPDPLASSIKQSCEAAIRKAFPSGDYKISILELVREKKIAKRVHLELGQEALENVNH
                     IIAIASGKGGVGKSTVAVNLAVALAMKGLRVGLADADVYGPSVPKMTGTEDAQPSVED
                     FGGKEMIIPVEKYGVKWMSIGYFASAGQPLIWRGPMASNALKQMVLQVKWGELDFLLI
                     DLPPGTGDIHISTVQDIPLSGAIVVSTPQAVAISDVEKGVNMFRNKDINKPVLGLVEN
                     MAWFTPEELPANKYYIFGKDGCKAMAEKFNVPLLAQIPIVQSIRESGDNGMPVTLMER
                     IDSMAFRELADKVVGMKL"
     gene            complement(4124..>5057)
                     /locus_tag="P5293_07000"
     CDS             complement(4124..>5057)
                     /locus_tag="P5293_07000"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR49682.1"
                     /translation="AIASQFSDVEKAFVSGDDVTANTTAQNLREKAINFYNNYSPEVD
                     KKVAKTFLKIFREKVSADNLPDIYSLIDEKFGGNVDEYVDYLYDKSVFTSMDKLYRAI
                     ENGREAVINDPAVIIGRSLFSLMNQLSIKLNRINSLYAEASKKYLAGLLEMNKGKAMY
                     PDANSTMRLTYGKVLNYSPKDAVLYEHYTTLKGVMEKEDPNNWEFVVPAKLKELYNNS
                     NYGPYAMKDGKLPVAFITNNDITGGNSGSPVLNAKGELIGLAFDGNWEAMSGDIIFEP
                     NLQRCINVDIRYVLFIIDKFGGAGYLLNEMSIVK"
BASE COUNT         1448 a          947 c         1024 g         1538 t
ORIGIN      
        1 taatggtttt ttagtttaag ttctatgctg caaattcata ccaatcaaaa ggtgtcaatc
       61 tgtcaagact ctgatgagtt tgctgatata taaatgttgt caagcgccct gcctcctcgt
      121 caggggtctt gctcgagaag atctcagaac tgcgacagaa aaactctctc ttccattaaa
      181 agaactctta tcaccatcaa ttatggcttt ggtaagcatg agttcttcgc tatcttgttt
      241 catatgttac aaatataatg aatttacaat cccctcattt gtcaatttat tatctttgca
      301 agaattattg aataattatt aaaggatgta cagaacacac acttgcggag aattacgcat
      361 aaatgatgca ggcaagcagg ttatgcttgc cggttgggtt cagagaatta gaaagatggg
      421 tgggatgagt tttattgatc ttagagacag atatggtata actcagttga cagttgatga
      481 gagtgattca aaggaactta atgctgttgc agaaaaattg ggtagagaat acgtgattca
      541 ggctacaggt gaagttgcag agaggacaaa caaaaaccca aaaatttcta ccggagatat
      601 tgaagttaag ctttcagcca ttaaggtgct taatccttca ctgattcctc cctttactat
      661 tgaagatgaa agtgacggag gtgaggagct tcgtgccaaa tttcgctacc tcgatcttcg
      721 tcgcaatcca cttagaaagg cactcgaact tagacacaaa ttggctcagg aaaccaggag
      781 atatcttgat gaacacggtt ttatggagat agaaactcct tttcttatca aatctactcc
      841 agaaggagct cgcgattttg ttgtgccatc gagaatgaat cccggagaat tttatgcact
      901 tccgcaaagt ccgcagcaac agaagcaact tttgatgata gcaggatttg acagatattt
      961 tcaaatagtt agatgttttc gtgatgagga tctcagggcg gacaggcagc cagagtttac
     1021 acagattgac tgtgagatgt catttgtcga gagagaagat attctctgca tgtttgaggg
     1081 gatgattaag tctcttttca aaaatgtttt gggaagagat tttaattatt cttttccccg
     1141 tatttcctat tcggaagcaa tggacgaata tgggtctgat aaacctgaca ttcgttttga
     1201 aatgaaaatt aaggagatta caaatattgt acagggtaag ggtttctcag tttttgatgc
     1261 agccgaatat attggtgcaa tagtcgttaa taattgcgta tcttatacgc gcaagcaact
     1321 tgatgagttg actgaatggg taaagcgacc tcagattgga gcaaaggggc ttgtatatgc
     1381 caggtatact gaggatggaa ttaaatcgtc tgtagataaa tttttctctc cagaagaatt
     1441 gtcgagttta tgtaaagtca ttgatgccaa agttggagat cttgttctga ttttatgtgg
     1501 caacaggaaa aagacgcaag aagcacttgg aactctcaga atagagatgg gtaatcgtct
     1561 tggcttaaga caaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttggg
     1681 atattctgct gaagaggttg aatataaatt cggctttatg ataaatgcat tcaaatacgg
     1741 agcgccacca cacggaggaa tagctttcgg ttttgacagg ttgtgtgctc tttttggggg
     1801 ccaggaaagc attcgtgatt tcatagcttt tcctaaaaat aattcaggta gagatgttat
     1861 gttagatgca ccttccagaa tagacaaggt tcagctggat gaactttttc tcaggagcac
     1921 atttgaaggc aactgattta tataaataca tacagatatg ggcatgttga ttttttatct
     1981 actgttagcc ctttcggtct cttttttatg ctctattatg gagaccgtgc tgcttacagc
     2041 ctctgtgtca ttcttgaaca tgaaggtcaa tcagggtatt aaggcagctt caatattaag
     2101 agaccagaaa cagaagattg ataggccaat agccgccata ctctctctta acactgttgc
     2161 tcatactgta ggcgcttctg gagtaggtgc ccaggcgacg gcgttgtttg gagatgctta
     2221 ttttggaatt atttctgcca tccttacact cctgatcctt gttttgtctg aaatacttcc
     2281 aaagagtgtc ggggctcact attggaagca tcttgttgtt ccggccgggt atatcatacg
     2341 atgcatgata gttatagcat atcctctggt aattctttct gagcaaatga cacggcttat
     2401 atcaagaggt tctaaggatg aggccactgt cagtcgcgag gaggtagcag caatggtaga
     2461 cattggtgta gaggagggaa cttttgaaga tactgaaaag aaaattattc agaatttgat
     2521 aaagctcaga agcgtgaggg ctagtgatgt tatgactccg agagttgttg tggctactgc
     2581 atcagaggag attactatag gggaattcta caaaaacaaa acatatctgc attattcaag
     2641 aataccagtc tattcagaca acgacaagga gaaaatatgt ggatttgttt acagacaaga
     2701 agttttggaa catcttgcta atgataattt tgatctttgt ctcaaggaaa ttaagaaacc
     2761 cattatgatt gctccgacaa gtcagccact caccattatg tgggagaggt tgacatcgca
     2821 aaaaacacat attgccatga ttgtggacga gtacggaggt tttgagggaa ttgttactct
     2881 tgaggatatc attgagacta ttatggggat tgagattatg gatgatagag acatagtcgc
     2941 cgacatgcag caatttgcac gagagcgttg ggaggagagc gtcaagaaaa gcacaaatac
     3001 ttgagttaat attacaactt cattccaaca actttgtctg caagttctct aaaggccata
     3061 ctgtcaattc tttccatgag tgtcacaggc attccgttgt cgccgctctc cctgatgctt
     3121 tgaacaatag gtatctgagc aagcagagga acgttaaatt tttctgccat agccttgcaa
     3181 ccgtcttttc caaatatata atatttattt gcaggtaact cttcaggagt gaaccatgcc
     3241 atattctcta ccaggccaag cacaggttta ttaatatcct tgtttctgaa catgttaacc
     3301 cctttttcga catcagagat tgctacagct tgaggagtgc ttaccacaat agctcctgaa
     3361 agtggaatgt cctgaactgt agaaatatga atatctccag ttcccggagg aagatcaatc
     3421 agtaaaaagt caagctctcc ccatttaact tgcaatacca tctgcttgag tgcattgctt
     3481 gccattggac ctctccagat aagcggctgg cctgcacttg caaaatagcc tatggacatc
     3541 cattttacac catatttttc aacaggtata atcatctctt ttccaccaaa atcttcaacc
     3601 gaaggctgag cgtcttcggt acctgtcatc tttggaacag aaggaccgta aacatctgca
     3661 tccgcaagcc caacgcgcaa tcctttcata gccagggcta cagcaagatt aacagcaaca
     3721 gttgacttgc caactccgcc tttacctgag gcaattgcaa ttatgtgatt tacattctca
     3781 agagcctcct gcccaagttc caaatgcact ctttttgcta tctttttttc tcttactaat
     3841 tcaaggatac ttatcttgta atctcctgaa ggaaaggctt tcctgattgc cgcttcgcaa
     3901 ctctgcttga ttgagctggc aagtggatct ggagatgaga agacaagttt aaaacgtatc
     3961 ttcccatctt caacttttat atcctcaacc atctcaagtg aaacgatgtc cttatcttta
     4021 gcaggatgga caactcctct cagaacatct tcaatctctt tttgtgtcat ttgaatattc
     4081 ttaaaaatct ttcgactaat caacaactct tgtaccaaag agtttatttg acgatagaca
     4141 tttcatttag cagatatcct gctccaccaa atttgtcaat gataaaaaga acatatctaa
     4201 tatcaacatt aatgcatctt tggagattag gttcaaaaat gatatctccg ctcatagctt
     4261 cccagttccc atcaaaagca agcccaatga gttctccttt tgcatttaat accggacttc
     4321 ctgaattgcc cccggtaatg tcattgttag taataaatgc tacaggaagt tttccatctt
     4381 tcattgcata aggaccataa ttgctgttgt tgtaaagttc tttcaattta gcaggaacta
     4441 caaattccca attgttggga tcctcttttt ccattactcc tttgagagtg gtataatgtt
     4501 catataaaac agcatctttg ggagaataat tcagcacctt cccgtaggtc agacgcatgg
     4561 tagaatttgc atccggatac atggcttttc ccttgttcat ctcgagtaag ccggcgagat
     4621 atttttttga tgcttcagcg tagagagagt ttattctatt gagctttata ctgagttgat
     4681 tcattagcga aaatagactt ctgcctatta tgactgcagg atcatttatt acagcctctc
     4741 ggccattttc aatggctcta tagagtttgt ccattgatgt aaatacggat ttgtcataga
     4801 gataatcaac atattcatca acattgcctc caaatttctc gtcaattaag gaataaatat
     4861 ctggaagatt atcagcagaa accttttccc ggaaaatctt aagaaatgtt tttgcaactt
     4921 ttttgtcaac ttcaggagag tagttgttat agaaattaat tgctttttct ctgagatttt
     4981 gcgcagttgt gttggcggtg acatcatctc cgcttacaaa tgccttctca acatctgaaa
     5041 actggctggc tatagct
//
LOCUS       DAOTQL010000119         5019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_13278_length_5019_cov_4.690572, whole genome shotgun sequence.
ACCESSION   DAOTQL010000119 DAOTQL010000000
VERSION     DAOTQL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13278_length_5019_cov_4.690572"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1797)
                     /locus_tag="P5293_07005"
     CDS             complement(67..1797)
                     /locus_tag="P5293_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRR49683.1"
                     /translation="MNKTIKFLFFSLLLIILSSFTPQKTKKHEGKVVVYIYATNDVHG
                     HFFDSTYNGSVNESSMSKVSGFINKSRLNLGEQSVILLDIGDNLQGDNSVFYYNNIDT
                     LSKNLVSEAFNFIKYDAVVVGNHDIEAGHRVYDKVKRELKAPYLAANAVSTTTGCPYF
                     QPYTIINKNGVKIAVIGFTNANIKRWLSSELYEGIDFLEIFPLIEKYIADIRRKESPD
                     LVVAAMHAGLGKENYYQLEDPSKYIAAHVKGIDLIFAAHDHNTFAGFIKNEQDSVPVV
                     EGGSKAQNLSLASVEMEFKRGKVVNKKITVSVIPLKDFKSDSVYNRYFSERFNNVKKF
                     SNSVIGYIDKGFSSKDAFFGSSEYVDMIHSIQLENSKADISFAAPLSFDITINAGPVS
                     FQSLTNIYPFENQLYVIKLSGKEITNYLEYSYRLWVNKVFSPKYPMLNIYTDENGVTR
                     FRNMHFNFDSAAGIIYKVDVTKGYGERINIISMTDGTPFNLEKVYEVALSSYRASGGG
                     DLLTLGAGIPTEQLNERVVMRMGDIRDMIYAKLKKEGKISPAKLNNWKFVPDSIVKER
                     IIIERAKLFE"
     gene            complement(1794..2576)
                     /locus_tag="P5293_07010"
     CDS             complement(1794..2576)
                     /locus_tag="P5293_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRR49684.1"
                     /translation="MKIALCQTTTVWEDIHTNLVNSQTIVEELFEKSPNTDIIIFPEF
                     FTTGFSVENNSIAEDINGPSVEWMREMSEKYSAAFLASIPIFQNGKYFNRAFFVSPEK
                     EWYYDKRHLFSYGGENRLYSQGEKRVIVNYKGWRILLQICYDLRFPVWSRNVALGYDL
                     IINVANWPSSRESVVPSLVRARAAENLSYYAFVNRAGEDPNCQYNRIGFVSNFKGDKI
                     PPVLVDDKNGIWTIYDLDLDSLRLFRETFRVWEDADKFSLYL"
     gene            2688..>5019
                     /locus_tag="P5293_07015"
     CDS             2688..>5019
                     /locus_tag="P5293_07015"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRR49685.1"
                     /translation="MLVNFTIHYTTKWGERLFVTGNISELGNANLREAIPLNFDNFYW
                     SKEIEIDSSEDFSYSYIIIDDSNNIFCESSPPRFFKHQGFVKYSLMDEWQNFSDETPF
                     MSNAIRKVLLNRTDTNYQKTENKNLIIRASVTNIAKEEDLCIVGSTDYLGFWSPERAV
                     TLYPDGDGSFTVYLDTKLLPDHFEYKFIVKSRLTGDFIWEEGENRKLNITDLSKTTLI
                     IRTHRCINLPIKKIRICGTAVPVFSLRRINDDGIGDFGSLYLMIDFLSTTNQSILQLL
                     PVNDTTLTHSWQDSYPYSPISVFALNPLYINLKELGQLHDSEFLKNYTKKSRKLNALK
                     AIDYEGVDNLKWSYLKKSFEENWQSLKYSEEFKKFLKQNFDWLLPYAAFSFLRDKFKT
                     PDFKLWPEYSVYDKNAIIKAIEVDTDFANGINLHYYVQFHLYAQLEKVHNYATEKGII
                     LKGDIPIGVNSFSVETWSEPNIFNMNQQAGAPPDSFSESGQNWGFPTYNWNNIDFEGY
                     MWWRRRLMNMESFFDAYRIDHILGFFRIWEIPLPFKSSIMGHFNPALPFSKEEIEGYG
                     LHIDFEKDYSHILLDDLNSTDVLFVQDPYTTDKYHPRIMSQFTSRYKKLTNKERENYD
                     RLYSDFFYRRNNDLWYQSAIKKLPPLISATGMLACGEDLGMIPSCVPDVMGKLRILSL
                     EIQRMPKNNNVLFGNPDTYSYFSVCSTSTHDISTLREWWETLGKEKEIYYHSNLHFTD
                     KAPKSCKIAICQEIIRQHLESSSMIAIFPIQDWMSLD"
BASE COUNT         1676 a          899 c          788 g         1656 t
ORIGIN      
        1 gttaagataa ccgttattag agaaacaaga gctgtaagtt ttgctaaata agcagcagtt
       61 ttaaagttat tcaaatagct ttgctcgttc aataattatt ctctccttta cgattgaatc
      121 aggaacaaat ttccagttat tcagttttgc aggagagatt ttaccttcct ttttaagttt
      181 ggcatatatc atgtctctga tgtcacccat tctcataaca actctttcat taagttgttc
      241 tgtagggatt ccggctccta aagttaaaag atcaccacct ccacttgctc tataagatga
      301 aagtgcaacc tcatatactt tttctaagtt aaaaggtgtt ccatcagtca ttgaaataat
      361 attaatccgt tccccgtaac cttttgtcac atcaacttta tatattatcc cggcagcaga
      421 atcaaaatta aaatgcatgt ttctgaacct tgtcacccca ttttcatcag tataaatatt
      481 taacattgga tatttaggag agaatacctt attaacccat agtctataag aatactcaag
      541 atagttcgta atctctttgc cagaaagttt tatgacatac aactgatttt caaacggata
      601 tatattagtt aggctctgga aactgaccgg tcctgcatta attgtaatat caaaagaaag
      661 aggtgcagca aaagatatgt ctgctttgga attttccagc tgtatagaat ggatcatgtc
      721 aacatattca gatgaaccaa aaaaagcatc ttttgaagaa aagcctttgt caatataacc
      781 tataacacta tttgaaaatt ttttgacatt attaaacctc tcagaaaaat atctgttata
      841 aacagaatct gacttaaaat ctttcaatgg aataacagag acagttattt ttttatttac
      901 cactttcccc cttttaaatt ccatttcaac agaagccaat gaaaggttct gagccttgct
      961 tcctccctca acaacaggca ctgaatcctg ttcatttttt ataaaacctg caaaggtgtt
     1021 atgatcgtgt gccgcaaaaa ttaaatcaat ccctttaaca tgagctgcaa tatattttga
     1081 aggatcttcc agctggtaat aattctcttt cccaagccct gcgtgcattg ccgcaactac
     1141 taaatccgga gattcttttc tcctaatatc agcaatgtat ttttctataa gtggaaatat
     1201 ctccaaaaaa tctatacctt cataaagttc agaagagagc catcttttga tattggcatt
     1261 agtgaatcct ataacagcaa ttttgacccc attcttatta atgatagtgt atggttgaaa
     1321 atatgggcat cctgtagtgg tacttacagc atttgctgca agatatggcg ccttaagttc
     1381 tcttttaacc ttgtcataaa cccgatgccc tgcttcaata tcgtggttac cgacaactac
     1441 tgcatcatat ttaataaaat taaaagcttc tgaaactaaa tttttagata aagtatcgat
     1501 attattataa taaaatactg aattatctcc ttgtaaattg tccccaatgt cgagtaaaat
     1561 caccgattgt tctcctaaat tcaatctgct tttattgatg aaacctgaaa ctttactcat
     1621 ggagctctcg tttacactgc cattgtatgt cgaatcaaaa aaatgaccat ggacatcatt
     1681 ggttgcataa atgtaaacta ctactttacc ctcatgtttt tttgttttct gaggagtaaa
     1741 agaagataga atgataagta gtaaagaaaa aaatagaaat ttaattgtct tattcataga
     1801 tataatgaaa atttatctgc gtcttcccat acacgaaatg tttcccggaa taatctcaaa
     1861 ctatctaaat ctaagtcata gatagtccaa atcccatttt tgtcatcaac aagaactggt
     1921 gggattttat ctcccttaaa attcgaaaca aaaccaattc tgttatactg acagtttgga
     1981 tcttctcctg ccctgtttac aaaagcataa taagataaat tttcggctgc tctggctctc
     2041 accaaagatg gtactaccga ttctcgagag gaaggccaat tggcaacatt tattattaag
     2101 tcatatccca gagcaacatt tctggaccag accggaaatc tcaaatcgta acaaatctga
     2161 aggagtatcc tccaaccctt gtaatttact ataacccttt tctccccttg tgaataaagt
     2221 ctgttttcac ctccataact aaacaaatgc cttttgtcgt aataccattc tttttctgga
     2281 gagacaaaaa aagccctgtt gaaatattta ccattttgaa aaataggaat tgatgctaaa
     2341 aaagcagcag aatatttctc gctcatttct ctcatccatt ctacagaagg tccatttatg
     2401 tcttctgcaa tactgttgtt ttcaacagaa aaaccagtag taaaaaactc gggaaaaatt
     2461 attatatctg tattgggaga cttttcaaac aactcttcaa ctattgtctg actgttaacc
     2521 agatttgtat gtatatcttc ccaaactgta gtagtctggc agagcgctat tttcatttta
     2581 tagttaatgt cgttacaaag ataatgaatt aaggaagtgg cgaagcttta attggtctca
     2641 aaatttcgtt aaatttgtaa ttattttaac agattgttca tgatcatatg ttagttaact
     2701 tcactataca ttatacgaca aagtggggtg aaagactatt tgttactggt aatatttccg
     2761 aactaggcaa tgccaatctt agagaagcta ttcctctgaa tttcgacaat ttttactgga
     2821 gcaaagaaat tgaaatagat tcttcagagg atttttctta ttcttatatt atcatagatg
     2881 attctaacaa cattttttgt gaatcttctc ctccaagatt tttcaaacat cagggatttg
     2941 taaaatattc ccttatggat gaatggcaaa attttagtga tgagacacca ttcatgtcca
     3001 atgcaattag aaaggtactt cttaatagaa cagatactaa ttatcaaaaa acagaaaata
     3061 aaaatcttat tatcagagcc tcagtaacca acattgctaa agaagaagac ctatgcattg
     3121 tgggttcaac tgattatctt ggtttctggt cgcctgaaag agcagttaca ctgtaccctg
     3181 acggtgatgg aagttttact gtctatcttg acacaaagtt gcttcctgat cattttgaat
     3241 ataaatttat tgttaaatca agactcactg gagattttat ttgggaagaa ggagaaaata
     3301 gaaaactcaa tattactgac ctgtccaaaa cgactcttat tattagaact cacagatgta
     3361 ttaaccttcc aataaaaaag atcagaatat gtggaacagc tgtccctgtt ttttctttaa
     3421 gaagaataaa cgatgatgga attggtgatt tcggttccct ttatttgatg attgactttc
     3481 tttccacgac aaaccaatca attcttcaat tacttccggt caacgacacg acgttaactc
     3541 attcctggca agactcgtat ccatacagcc ctatttctgt atttgcccta aatccccttt
     3601 atatcaattt aaaagaatta ggacaattgc atgattctga atttttgaaa aattatacta
     3661 aaaaatccag gaaacttaac gcattaaaag caattgatta tgaaggtgtc gataacttaa
     3721 aatggtcata tttgaagaaa agtttcgagg agaattggca gagtcttaaa tatagcgaag
     3781 aattcaaaaa gtttttaaaa caaaatttcg actggctttt accatatgca gccttcagct
     3841 ttttaagaga taaatttaaa acacctgatt ttaaattatg gcctgaatac tccgtatatg
     3901 ataaaaatgc tattattaaa gccatcgaag tagatactga ttttgcaaat ggcattaatt
     3961 tacattatta tgttcaattt cacctctatg cacagcttga aaaagtacat aattatgcaa
     4021 ctgaaaaagg tataattctt aaaggagata tcccaatagg ggttaactct ttctctgttg
     4081 aaacttggtc ggagcctaat atcttcaata tgaaccagca agcaggagct ccgcctgatt
     4141 ctttttctga aagtggacaa aattggggat ttcctaccta taattggaat aacattgact
     4201 ttgaaggtta tatgtggtgg agaagacgat taatgaatat ggaatcattt ttcgacgcat
     4261 atagaattga tcatatcctg ggtttcttta gaatttggga aattcctctt cctttcaaga
     4321 gtagtataat ggggcatttc aatccagcac ttcctttttc aaaagaagaa atagaaggtt
     4381 atggattaca tattgatttc gaaaaagatt acagtcatat tttacttgat gatttaaatt
     4441 ctacagatgt actattcgtt caagatccat atactactga taagtatcac ccgagaatta
     4501 tgtcgcaatt tactagtaga tataaaaaac tgacaaataa agagagagaa aattacgata
     4561 gactttatag cgattttttc tacaggcgaa ataatgattt atggtatcaa agtgcaataa
     4621 aaaaacttcc ccctctgatt tctgcaacag gaatgcttgc ctgtggcgaa gatcttggaa
     4681 tgataccttc atgtgtacct gatgttatgg gcaaacttcg aatattgtca cttgagatcc
     4741 agagaatgcc aaaaaataat aatgtcttat ttggaaatcc cgatacatac agctattttt
     4801 cagtatgttc taccagtact catgatatca gcactttaag agagtggtgg gaaactcttg
     4861 gtaaagaaaa agagatttat taccacagca atttacattt cacggataag gcacctaaat
     4921 cctgtaaaat agcaatttgt caggaaatta ttcgccaaca tcttgaatcc tcttccatga
     4981 tagcaatttt cccaattcag gactggatgt ctctagata
//
LOCUS       DAOTQL010000120         4974 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_13416_length_4974_cov_5.576540, whole genome shotgun sequence.
ACCESSION   DAOTQL010000120 DAOTQL010000000
VERSION     DAOTQL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13416_length_4974_cov_5.576540"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..922)
                     /gene="uppS"
                     /locus_tag="P5293_07020"
     CDS             complement(191..922)
                     /gene="uppS"
                     /locus_tag="P5293_07020"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRR49686.1"
                     /translation="MTKVPRHVSIIMDGNGRWAKARGLDRIDGHREGTESVRAATEFA
                     AEKGIEYLSLFAFSEENWGRPKEEVDGLMNLMVNAIVNETPTIMKNNINFRAIGDLSK
                     LPADVLKSIRATEELSSINTGLRLIIFLSYSGKWDILQAATMYAEEVLNAYKDGLEPH
                     ELTNEYFSSLLSTNGIPDPDLIIRTSGEQRISNYLLWQCAYSEFYFTEVLWPDFRKTH
                     FQQALDLFDKRERRFGKTGNQVHNY"
     gene            complement(931..>1206)
                     /locus_tag="P5293_07025"
     CDS             complement(931..>1206)
                     /locus_tag="P5293_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRR49687.1"
                     /translation="WTVAPIAPHNLNVRPMIVPDDVSLEMSANSRSGKVILCLDSREF
                     ILPSGSPVKIKKADFPLKYISLQEGSFINALKDKLRWGEDKRNIQSL"
     assembly_gap    1207..1306
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1396..2115
                     /locus_tag="P5293_07030"
     CDS             1396..2115
                     /locus_tag="P5293_07030"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRR49688.1"
                     /translation="MTKLSVNINKFATLRNSRGGNLPDVIKAAIDCESFGAEGITVHP
                     RPDERHIRYKDARDLKKVVTTEFNIEGNPIDSFIELVLEIHPAQVTLVPDAHDVITSN
                     CGWDTKKNAAFLKKICKMFNEEGIRTSIFVNPDIEMVKYAAETGCKRVELYTEAYAAN
                     YSKNREEAIEDYIKAAEEALRVGLGLNAGHDLNLDNLHYFYKNIPGLLEVSIGHALIC
                     DAIYLGLENTIQSYLRELRNY"
     gene            2143..2754
                     /locus_tag="P5293_07035"
     CDS             2143..2754
                     /locus_tag="P5293_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRR49689.1"
                     /translation="MKKASLITEIVLAIAVVVLFILHFTSTSRSSGNDTQSGSGPVAG
                     SIVYIQMDSLVKQYDMFNDLKSELESKAQVIQDDLNKKGMAFERDVKDFQEKIQKGLI
                     TRSQAESQNAQLQNRERELQNYTQQKQMEMEEEQAVLIRKVMDALNKYLVKFNAEKKY
                     ALILNTSGTMNTVLQGDSTLNITNAVVAGLNEEYVANKGKKMK"
     gene            2765..3883
                     /locus_tag="P5293_07040"
     CDS             2765..3883
                     /locus_tag="P5293_07040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRR49690.1"
                     /translation="MKIAILSCFYPYRGGIAQFNANIYRELSKSHEVRAFNFKRQYPK
                     LLFPGKTQFVEKEDTAVKIDSTALLDTANPLSYITTARKIREWHPDLLLMRYWMSYFA
                     PSQGYVARHMSSNTKVVSIMDNVIPHEPRFFDTPFTKYYIGSNDGFVVLSDAVGYDLL
                     TFKPDAKYICLPHPLYDHFGNKVERSFAEKKLNLEEGKRNILFFGLIRDYKGLDILLE
                     AFGGLDERYQLIIAGEPYGSFEKYDSIIRNIPNSCRIKLFTHYISDREVPLFFSASDV
                     CVLPYRSATQSGISAISYHFDVPMITTNVGGLKEAIEVPGTGIVVKRVDPVLIREAIE
                     EFFNKELGCKFKENIAVQKDRLSWNNFADRLLDFYLTL"
     gene            3900..4850
                     /locus_tag="P5293_07045"
     CDS             3900..4850
                     /locus_tag="P5293_07045"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRR49691.1"
                     /translation="MKAITRTDFNFPKQSGKYVGKVRDVYTIGNTYLVMIATDRISAF
                     DVVLPKGIPYKGQVLNQIASKFLDTTSDIVPNWKIASPDPMVTVGYKCDPFPVEMIVR
                     AYLTGSSWRAYKAGAREICGIKIPDEMKEHQKFEYPIVTPTTKAEIGMHDQDISKEEI
                     ISRGIVKEDEYLMLEKYALDLFERGSEIASERGLILVDTKYEFGKKGDKIYLIDEIHT
                     PDSSRYFYKDSYEELFEKGEQQRQLSKEFVREWLMENNFTGQPGQKIPEMTDEIIKSI
                     SDRYIELYEKITGSKFEKAEYSDNIYERIETNILNMLARL"
BASE COUNT         1595 a          896 c          933 g         1450 t
ORIGIN      
        1 aacctgtggc ctgttataat caacttctat agttcgggca aaagttgaat cctgtgcttg
       61 gacccgaatt ggtaatagaa aacatgttaa gaaaaaaagt accggaaaaa aggatagtga
      121 cttcatctgc aatctctggc tgtaaaagcg caaatataag cttttatttg tattcacaaa
      181 tttggtttga ttaataatta tgtacctggt tccctgtctt tccaaacctc ctttctcttt
      241 tgtcaaagag atcaagagcc tgttgaaagt gagttttgcg aaaatcgggc caaagcactt
      301 cagtgaaata aaattcactg tatgcacatt gccatagtaa atagttgctt attctctgtt
      361 caccactcgt tctgataata agatcaggat ccggtatgcc gttcgtactg agaagagagc
      421 taaaatattc atttgtaagt tcatgtggtt ccaacccatc tttatatgcg tttaagactt
      481 cttcagcgta cattgttgcc gcttgcaaaa tatcccattt accactgtaa cttaaaaaaa
      541 taatcagtct taagccggta ttgatgctcg aaagttcttc agtagctcga atagacttca
      601 aaacatcggc aggtaacttt gaaagatcgc ctattgctct aaagttgatg ttgtttttca
      661 taattgtggg agtctcgttg actatcgcat ttaccatcag attcatgaga ccatcaactt
      721 cttccttagg tctaccccag ttttcttccg aaaaagcgaa aaggctcagg tattcaattc
      781 ctttctctgc ggcaaattct gtagctgccc gaactgattc agtgccttcc ctgtgtccat
      841 ctattctatc aagccccctt gccttggccc aacgtccatt tccatccatt atgatagata
      901 catgtcttgg aacttttgtc ataactgcaa ttataaagat tgaatatttc ttttatcttc
      961 tccccacctg agcttatcct tgagtgcatt aataaaactt ccctcctgca gggatatgta
     1021 cttcaaagga aaatctgctt tttttatttt caccggactt ccggaaggta atatgaattc
     1081 tctactgtca agacaaagaa ttactttgcc ggagcgcgaa ttggccgaca tctcaagcga
     1141 aacatcatca ggaactatca tcggcctgac atttaaattg tgtggagcta tcggggcaac
     1201 tgtccannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncact ccaattaata
     1321 tcaaaatctc tcgcaaataa agcaattttc attttagtgg tattattgtc aaaaatagat
     1381 aaatttgcag ttactatgac aaaattaagt gtaaatataa ataaattcgc tacactgcga
     1441 aattcaagag gtggaaatct tcctgacgtt attaaagcag ccatcgactg tgaaagtttt
     1501 ggtgcagaag ggattactgt gcacccaagg cctgacgaaa gacatattcg ctacaaagat
     1561 gccagagatc tcaaaaaagt tgtaactact gaatttaata ttgaagggaa tcctatcgat
     1621 tcttttattg aattagttct tgagattcac cctgcgcagg tgacgcttgt tcctgatgcc
     1681 catgatgtga ttacttctaa ttgtggatgg gataccaaaa aaaatgctgc ctttctcaaa
     1741 aaaatatgca aaatgttcaa tgaagaagga attcgaactt ctattttcgt aaatcccgat
     1801 attgagatgg taaaatatgc tgcagaaaca ggctgtaaaa gggttgaact ttatactgaa
     1861 gcatatgcag ccaactattc aaaaaacagg gaagaggcta ttgaagatta catcaaggca
     1921 gcagaagaag cactaagggt tggtttggga ttgaacgccg gacacgatct aaatcttgat
     1981 aacttgcatt atttctataa aaatattccg gggttgcttg aagtttcaat aggtcatgct
     2041 ttaatttgtg acgcaattta ccttggcctg gaaaatacaa tccagtccta tttgagggag
     2101 ttaagaaatt attaatatat ttgctttgta aatagtttaa aaatgaaaaa agcctcttta
     2161 attaccgaaa ttgtgttggc tattgcagtc gttgtgctct ttatccttca ctttacttca
     2221 acttccagat catctggtaa cgatacacaa tcaggctccg gccctgttgc gggcagtata
     2281 gtctatatcc aaatggactc tcttgtcaaa caatatgata tgtttaacga tctcaaatct
     2341 gaacttgaat ccaaagctca agttatacag gatgacctca acaaaaaggg aatggctttt
     2401 gaaagagatg ttaaagattt ccaggagaag atacaaaaag ggctgattac tcgctctcaa
     2461 gcggaaagtc aaaatgctca acttcaaaat cgagaaagag aacttcaaaa ctatactcaa
     2521 caaaaacaga tggagatgga agaagaacaa gcagttctga taagaaaggt tatggatgct
     2581 cttaataaat accttgtcaa gttcaatgcc gaaaaaaaat atgcacttat cctcaacaca
     2641 tccggcacta tgaacacagt acttcaggga gactccacgc ttaatataac aaatgcagta
     2701 gtggcaggcc tcaacgaaga gtatgttgcg aacaaaggta aaaaaatgaa ataactgcac
     2761 ttttgtgaag attgcaatac tatcttgctt ttatccttac cgaggtggta tagcacagtt
     2821 caatgcaaac atatacagag aactgagcaa gtctcacgaa gtaagggctt ttaatttcaa
     2881 gagacaatac cccaagctgc tatttccggg taaaacacaa tttgttgaaa aagaagatac
     2941 tgcagttaag attgacagta ctgccttgct tgacacagcc aatcccttaa gttatattac
     3001 aacagccaga aaaattaggg agtggcatcc agatctgtta cttatgcgtt actggatgtc
     3061 ctattttgca ccttctcaag gttatgttgc tcgtcacatg tcatctaata ccaaggtggt
     3121 ctcaataatg gacaatgtca ttccccatga accaagattt tttgataccc cttttactaa
     3181 atactacatt ggttcaaacg atggttttgt agtcctttct gatgctgtcg gatatgattt
     3241 acttactttt aagcctgatg caaagtatat ctgcctgcca catcctcttt atgatcactt
     3301 tggaaataag gttgaaagat cttttgcgga aaaaaaactt aacttggaag aaggaaagag
     3361 aaatattctt ttctttggat taataaggga ttacaaaggt ttggacatct tgcttgaagc
     3421 atttggaggc ctggatgaaa gatatcaact tattattgct ggtgaaccat acggaagttt
     3481 cgaaaaatat gattccatta tccgaaatat acccaatagc tgcagaatca agctgttcac
     3541 tcactatatc tcagacaggg aggtacctct ttttttctct gcaagcgatg tatgtgtcct
     3601 gccataccgt tctgcaacac aaagtggcat tagtgcaata tcgtatcact ttgatgtccc
     3661 aatgataact accaatgttg gcgggctcaa ggaagctata gaagttcctg gcaccggtat
     3721 tgtagttaaa agagtagacc ctgttttgat aagggaagct atagaggaat ttttcaataa
     3781 ggagcttggc tgcaaattca aagaaaacat agctgtccag aaagacagat tatcctggaa
     3841 taattttgca gatcgactct tggattttta tttaacatta taacatcata gaaattttta
     3901 tgaaagctat tacacgtact gactttaatt ttcctaagca gagtggtaaa tatgtaggga
     3961 aagtcagaga tgtttataca attggaaaca cttacttagt gatgattgca actgacagaa
     4021 tatcggcatt tgacgtggtg ctgccaaaag gtattccata caaaggacaa gttcttaatc
     4081 aaatagcgtc gaagtttctt gatactacct ccgatattgt acccaactgg aaaattgcct
     4141 ctcctgaccc aatggtaact gttggatata aatgtgaccc ctttcccgtg gagatgatag
     4201 taagggctta tcttacaggc agttcatgga gagcttataa agcaggtgct cgagagatct
     4261 gcggtattaa gattcctgac gaaatgaaag aacatcaaaa atttgaatat ccaattgtca
     4321 cacctactac aaaagctgaa ataggtatgc atgaccagga tatctcaaaa gaagagatta
     4381 tctccagagg aatagtgaaa gaggatgagt atttgatgct ggaaaaatat gctctcgatt
     4441 tatttgagag aggttcagag attgcttccg aaaggggact tatactggtt gatacaaaat
     4501 atgaatttgg caaaaaaggt gataagattt atcttataga tgaaattcat actcctgact
     4561 catccagata tttttacaag gatagctatg aagaactatt tgaaaaagga gaacagcaaa
     4621 ggcaactctc caaggagttt gttagagaat ggctcatgga gaacaatttc accggtcagc
     4681 ccggccaaaa gattccggag atgactgatg aaattataaa atcaatttct gatagatata
     4741 tagaacttta cgaaaagatt acaggcagca aatttgaaaa agccgaatac agcgataata
     4801 tttatgaaag aatcgaaaca aatattctaa atatgctcgc aagattatga gattgactca
     4861 caaaatattg atttcattcc ttttttctct cataattttt tctgcttacg gacagaatca
     4921 gcctgtgaaa gtggaaatct ccaaagaaaa gattaaaaag gaaggtgtta attt
//
LOCUS       DAOTQL010000121         4948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_13499_length_4948_cov_4.944410, whole genome shotgun sequence.
ACCESSION   DAOTQL010000121 DAOTQL010000000
VERSION     DAOTQL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13499_length_4948_cov_4.944410"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="P5293_07050"
     CDS             <1..267
                     /locus_tag="P5293_07050"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRR49692.1"
                     /translation="GKINYVKYSYAGSEVKKVPGLGTFNTLKVEAKPVAGHAFKGNEV
                     ISIWISADENRVPLIIEISIQVGYISARLAEYANLKYPLKSRVK"
     gene            264..692
                     /locus_tag="P5293_07055"
     CDS             264..692
                     /locus_tag="P5293_07055"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HRR49693.1"
                     /translation="MSKSDRIEHEGVVIDVNHNFVSVEIIRQSACSSCGAKGMCSAAN
                     SQNSVIQVPANGFELYKNGEKVKVVMKQSLGLKALWISYIIPLIILLILLLLLSTFKI
                     SEILIGLSIICALALYFLTIYLLRDNFKKEFVFTIEKLDE"
     gene            692..1552
                     /locus_tag="P5293_07060"
     CDS             692..1552
                     /locus_tag="P5293_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HRR49694.1"
                     /translation="MNTVLFTILSLTILGLLLALILYFVAQKFKVEEDPRIDEVQAIL
                     PGANCGGCGYAGCRAFAESCVRASNLDNHFCPVGGNDVMKQIAGYLGMEVAEKNPQIA
                     VVRCNGSYDNRPKINFYDGYPSCAVMSSLYAGDTACRYGCLGLGDCEQACQFDAIKVN
                     VETGLPEVDESKCTGCGACVKACPKMIIELRSRGIKNRRIFVSCVNKDKGGIAKKACT
                     AACIGCTKCQKVCAFDAITISSNLAYIDYNKCKLCRKCVQECPTNSILEVNFPVRVTK
                     KEEESQLVEQ"
     gene            1566..2903
                     /gene="rsxC"
                     /locus_tag="P5293_07065"
     CDS             1566..2903
                     /gene="rsxC"
                     /locus_tag="P5293_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HRR49695.1"
                     /translation="MSKTFSIGGIHPDDNKISKDAAIEDFAPVKTTFISMSQHLGAPA
                     EPVVVKGDKVKVGQLIAKSSGFISANIHSSASGTVRSIEMLPDLAGNLVQHVVIDVEG
                     DEWMETIDRSKEIIRDIKLSKEEIIKRIADCGIVGMGGAAFPTHIKLTPPPGKVADML
                     LINGSECEPYLTSDYRIMLENPEEILIGARIMMKALNVKQAYVGIEDNKLEAIRSMEA
                     VADKFYPDIKIVSLKKRYPQGGEKQLIDAITGRRVPSMGLPIDVGVVVQNVGTAFAVY
                     EAVQKGKPLIDSIVTFTGKCVTEQKNYRIRVGTTFDTVIHATGGFPKEAAKLISGGPM
                     MGKAVSCLDAATVKSTSGLLFLTEEETKRKPENNCIRCAKCVAACPMGLQPFFLNKLA
                     LASRMDELEENMVQDCIECGCCMYTCPSHIPLLDVIRLAKNRVLKIMRSRPKN"
     gene            2936..3937
                     /locus_tag="P5293_07070"
     CDS             2936..3937
                     /locus_tag="P5293_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRR49696.1"
                     /translation="MKKLLVSPAPHIHGNQSTKILMRDVVIALIPSLLVSIYFFGFSA
                     IKLALVGVITCVGVEYVIQKYIIKCNVTIGDYSAVVTGLLLALNLPPNSPWWLIFIGS
                     IVAIGVAKQTFGGLGQNLFNPALVGRVFLLISFPVLMTDWTIPVSWFRGGVDATSGAT
                     ALSIIKEGLAQGLTLDQIFKANNFSYAQMLFARIGGSVGEVSALALIAGFAYLLYRKV
                     VSIHIPLSIIFTVFIFTGIFWLINPQQYTDPIFNLLTGGLLLGSFFMATDYVTSPMSK
                     KGMVVYGIGIGIITVLIRYFGSYPEGVSFAILIMNAFVPLINRFMKPAYFGKEVKNG"
     gene            3930..4532
                     /locus_tag="P5293_07075"
     CDS             3930..4532
                     /locus_tag="P5293_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HRR49697.1"
                     /translation="MAKESTLKNMVVVLSLICLISSSLLGGAYVLTKEPIEAARMSKI
                     NESIAKVVPSFDNNPNGEFFIKEFNGDSYKVYPAKKEGKIVGYAIETFSNAGFGGRIT
                     LMVGYRTDSTIVNTVVLSHSETPGLGEKMVEGKSNFSLQFIGKNPSEFKLSVKKDGGD
                     VDAITASTISSRAFCDALVSSYEVFKLCMADQPKKEVTNE"
     gene            4525..>4948
                     /gene="rsxE"
                     /locus_tag="P5293_07080"
     CDS             4525..>4948
                     /gene="rsxE"
                     /locus_tag="P5293_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HRR49698.1"
                     /translation="MSKWGLITKGFVKDNPIFVLVLGMCPTLATTTSAMNGLGMGIAT
                     TFVLVFSNVLISLVAKQVPDKVRIPVYIVLIAAVVTVLQLLMQAYTPALYETLGIFIP
                     LIVVNCIVLGRAEAFASKNSVVDSMLDGIGIGLGFTLSL"
BASE COUNT         1483 a          760 c         1111 g         1594 t
ORIGIN      
        1 ggcaaaatta attatgttaa atattcatat gcaggatctg aagtaaaaaa agttcccggc
       61 ctgggtactt ttaatacgct taaagttgag gcgaaacctg tggccggaca cgcatttaag
      121 gggaatgaag ttatttccat ctggatttca gctgatgaaa acagagtgcc tcttattata
      181 gaaatctcta tacaggttgg atatatttca gcaagacttg ctgagtatgc aaacttgaaa
      241 tatccattaa aaagcagagt aaaatgagta aatctgacag aattgagcac gaaggtgtgg
      301 ttattgacgt taaccataat tttgtttctg ttgaaataat tcgccaatca gcttgttctt
      361 cttgtggcgc aaaagggatg tgttcagccg ccaattctca aaatagtgtg attcaggttc
      421 cagccaatgg gttcgaactt tataaaaatg gagaaaaggt taaggttgtg atgaaacaaa
      481 gtttgggttt gaaggcactt tggatatctt atattattcc tttgattatt cttttgattt
      541 tattgttact tttgtcaact tttaaaattt cagaaatact tataggtttg tctattattt
      601 gtgctttagc cttatatttt ttgacaattt accttttaag ggataatttt aaaaaggaat
      661 ttgtatttac gattgagaaa ctggatgaat aatgaataca gtattattta cgattttgtc
      721 gttaacaatt cttgggcttc tgttggctct gattctctat tttgttgctc agaagtttaa
      781 ggtagaggag gatccgagaa ttgacgaagt gcaggcaatt ttacctggtg cgaattgtgg
      841 cggttgtggt tatgccggat gcagggcttt cgctgaatca tgtgtcagag cttctaatct
      901 tgacaatcat ttttgtcccg tgggcggaaa tgatgtgatg aaacaaattg caggctatct
      961 gggtatggaa gtggcagaaa aaaatcctca aatagcagtt gtaagatgta acggaagtta
     1021 tgacaacaga ccaaaaatca atttttatga tggatatcct tcatgtgcag taatgtcttc
     1081 tttgtatgca ggggatactg catgcagata cggatgtctt ggacttggag attgtgagca
     1141 ggcatgtcag tttgatgcta taaaagttaa tgttgaaacc ggattaccag aagttgacga
     1201 atcaaaatgt acaggttgtg gtgcatgtgt caaggcttgt ccaaaaatga ttatcgagtt
     1261 gagatccaga ggaataaaaa acagaagaat atttgtgtcg tgtgttaata aggataaggg
     1321 gggtatagct aaaaaagcat gtacagcagc gtgtatagga tgcacgaaat gtcaaaaggt
     1381 atgtgcattt gacgcaataa caatttcatc gaatcttgca tatattgatt ataacaaatg
     1441 taagctttgt cgcaagtgcg tacaggagtg tcctaccaac tcaattctcg aagttaattt
     1501 tcctgtgaga gttactaaga aagaagaaga gtcacagtta gtcgaacagt aagttacaag
     1561 tatatatgag taaaacattt tctattggag ggattcatcc ggatgataat aaaatatcta
     1621 aggatgctgc aattgaggat tttgcaccag ttaaaacaac ttttatttcc atgtcccaac
     1681 accttggggc tccagctgag cctgttgtgg ttaaaggtga taaggttaag gtagggcagt
     1741 taattgcaaa atcgagtgga tttatatccg ccaatataca ctcttctgca tcaggtacag
     1801 taagatctat tgagatgttg ccagatttgg ccggcaatct tgttcaacac gtagtgattg
     1861 atgtggaagg tgatgagtgg atggagacca ttgacagaag taaggagata atccgggata
     1921 taaagcttag caaagaggag ataattaaaa gaatagcaga ttgtggaatt gttggaatgg
     1981 gaggtgcggc tttcccaact catattaaat taactccgcc tccaggtaaa gttgctgaca
     2041 tgttgttgat aaacggatct gaatgtgaac cttatctgac ctctgattat agaatcatgc
     2101 ttgaaaatcc tgaggagata cttataggtg caaggataat gatgaaggct cttaacgtga
     2161 aacaagccta tgtggggatt gaagataata aactcgaagc cattagaagt atggaggcag
     2221 tagcagacaa gttttatcct gatattaaaa ttgtttcatt aaagaagaga tatccccagg
     2281 gaggtgagaa acaactaatt gatgcaatta cgggcagaag agtaccttct atgggcttgc
     2341 cgatagatgt aggagttgta gttcagaatg ttggaaccgc ttttgcagtt tatgaagccg
     2401 tacagaaggg gaaaccttta atcgattcca ttgtaacttt taccggaaaa tgtgttacgg
     2461 aacaaaaaaa ctacagaatc agggtaggga caacatttga tacagtaata catgcaactg
     2521 gaggttttcc gaaagaagca gcaaaactta tcagcggcgg accaatgatg gggaaagcag
     2581 tttcatgttt agatgcagct acagttaaaa gcacatctgg cttgcttttt cttacagaag
     2641 aagagacaaa gagaaaacct gaaaacaact gtataagatg tgcgaaatgt gttgctgcct
     2701 gtcctatggg tttgcaaccc ttttttctga acaaacttgc actcgcaagc agaatggatg
     2761 aactagaaga gaatatggtg caggactgta ttgaatgcgg ctgctgcatg tatacctgtc
     2821 cttctcatat accattgctt gatgtcataa ggctcgcaaa gaacagggta cttaagatta
     2881 tgagaagtag acccaaaaac tgaaaataag gtttggataa taaaatcatt aactaatgaa
     2941 aaaattactc gtttctcccg ctccgcatat tcatggtaat cagtcaacaa agattttgat
     3001 gagggatgtg gtgatagctt tgataccctc tttgctggtt tcaatctatt ttttcggttt
     3061 ttcagctatt aaacttgctt tagttggggt aataacttgt gtgggagttg aatatgttat
     3121 tcagaaatat attattaagt gtaatgtgac aattggtgac tattctgcag tagttacggg
     3181 attactttta gctttaaatc ttcctcctaa ttcgccatgg tggctgattt tcattggttc
     3241 tatagttgct attggagttg caaaacagac ttttggcggg cttggacaaa atctttttaa
     3301 ccctgctctg gttggacgtg tctttttact gatttccttt cctgtcctta tgacagactg
     3361 gacaattcct gtttcatggt ttagaggagg agtagatgcc acttcaggag ctacagcttt
     3421 atctataatt aaggaagggc ttgctcaggg attgactctt gatcagattt ttaaagcaaa
     3481 taatttctct tatgctcaaa tgttatttgc aagaatagga ggttctgttg gagaagtatc
     3541 tgccctggct ctaattgcag gatttgcata tctgctgtat agaaaagtgg tgagtattca
     3601 tattccttta tcaattattt ttactgtttt tatttttaca ggaatattct ggctaatcaa
     3661 tcctcaacag tatactgatc caatatttaa tttacttaca ggagggttgc ttttaggttc
     3721 gttttttatg gctaccgatt atgtgacctc tccaatgagc aaaaaaggga tggtagttta
     3781 tggtattggt attggtatta ttacggtttt aataagatat ttcgggtcat atcctgaagg
     3841 ggtttcattt gcaatattaa taatgaatgc ttttgttcct ttaatcaata gatttatgaa
     3901 accggcctat tttggaaagg aggtgaaaaa tggctaagga atctacatta aaaaatatgg
     3961 tggtagtgct ttctttgatt tgtctcatat catcttcact gttgggcgga gcatatgtgc
     4021 ttacaaaaga acctatagaa gccgccagga tgtctaaaat caacgaatct attgccaagg
     4081 tagtgccttc ttttgacaat aatcctaacg gcgaattttt tattaaggaa tttaatgggg
     4141 atagttataa agtttacccc gcaaaaaagg aaggtaaaat tgtcggttat gccatagaga
     4201 cattttctaa tgccggcttt ggtggaagaa taactctcat ggttggttac aggactgaca
     4261 gcacaattgt taatactgtt gtcctaagtc attcagagac acctggttta ggtgaaaaaa
     4321 tggttgaggg aaaaagcaat ttcagtcttc agtttattgg caagaatcct tctgaattta
     4381 aactctctgt caagaaagac ggaggtgatg ttgatgctat tactgcttcg acaatttcat
     4441 caagagcatt ttgtgatgcc ttggtatctt cttatgaggt atttaaacta tgtatggccg
     4501 atcagccaaa aaaggaggta acaaatgagt aagtggggtc ttataactaa gggttttgtt
     4561 aaggacaacc cgatttttgt attggttctg ggtatgtgtc ctactcttgc tactactact
     4621 tcagcaatga atggtctcgg tatgggtatt gcaactactt ttgtgctagt cttttctaat
     4681 gttcttattt cacttgtggc aaaacaggtg cccgacaagg taagaatccc tgtttatatt
     4741 gtgcttattg cagccgtagt aactgtactt caactgctaa tgcaggccta tactcctgct
     4801 ctttatgaga ctctgggaat atttatacca ctgatcgtgg ttaactgtat tgttcttggt
     4861 agagctgaag catttgcttc caaaaacagt gttgtggatt caatgcttga cggcataggt
     4921 attggtttgg gatttacatt gtcactta
//
LOCUS       DAOTQL010000122         4847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_13837_length_4847_cov_4.209725, whole genome shotgun sequence.
ACCESSION   DAOTQL010000122 DAOTQL010000000
VERSION     DAOTQL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13837_length_4847_cov_4.209725"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..882)
                     /locus_tag="P5293_07085"
     CDS             complement(73..882)
                     /locus_tag="P5293_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49699.1"
                     /translation="MRREILLVLLLTITFNSLCSQVNKGDSPFYEKMRTLPLEEREKL
                     IYDEIMSGNVPDYMKGFVKISYKDRDANHKTHRVTLFVKPDYLTVGEGKSAFIIPMTP
                     ATAQKIADSSGCSLPTPKIVDIIYKKSRLKVEPFNYIPRGDRNETPDIFYDHSRVIFA
                     QIKAAGYKPGVFIAGSKKDIVISSKLQDSLRPGHVIIYGWHRLDGTPIQPVYNGHLGR
                     YVDYSHGVRLICDTIKIDGKKYNYRDVLRDTLLYTLLSNEDKPLVITSYSY"
     gene            complement(889..1206)
                     /locus_tag="P5293_07090"
     CDS             complement(889..1206)
                     /locus_tag="P5293_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRR49700.1"
                     /translation="MKNLFKSISLILVASLFFFSNGANAQGKKAAKEMEVTFSVDIDC
                     PNCKKKLEDKLPFEAGVKDLKVNLENHTVWMKYDPSKTDKVKLAKAIEKLGYTAKEIE
                     VTK"
     gene            complement(1247..3448)
                     /locus_tag="P5293_07095"
     CDS             complement(1247..3448)
                     /locus_tag="P5293_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49701.1"
                     /translation="MKRIIITFLFIISITTLSFSQTIHGIVKAQHESGEIEPLPYASV
                     YWLEGKFSLETDQDGKFTIKNEHSGKVSLIATYIGYSRDTLILENGENKADFILKEEN
                     NSLDEIRVVGRQEGNYISKVTPVKTEVISAAGLCKMACCNLAESFENSASVSVGYSDA
                     ITGARQIRLLGLSGQYTQMLDENRPVMRGIAAPFGLSYIPGQWLESIQIAKGPSSVVN
                     GLEAITGQINMEHRKPTTEQPLFVNLFLSNMLRAEANVASSLQLNPKWSTVILGHFST
                     DPMEHDGNDDGFKDEPLTTQFNFSNRWLFAPSNGMQLRFGIKAMSDERIAGMNDFKNG
                     SKVTDDLWGSKIKNKGLNGYAKLGIPLNESNSKNVAAVVDYSMYDMNSFFGYKSYDAT
                     QNSVFFNLMLQNQINDNHKYIIGLSGQYDNFDEHLNGANVGREEQSAGAYGEYTYVNG
                     DKITVVAGLRLDNNNLHGWLFAPRINVKYDFTDKLVLRASAGRGFRSPNQIADNLGIL
                     STGRKIKMEEHPDMEEAWTYGMNLTGYLPIGFDKNATVSFEYFRTDFQHQLIVDQERD
                     LSTIWLYNLSGRSYTNTYQADFSIQPVERFTVLLTYRLTDAKVTLENQGLVERPLMSK
                     YKGVLNLQYATRMNKWTFDFTAQLNGPARLPNFMDKEYSPVYSVLFAQVTKKFKKLDI
                     YIGGENLTNYRQKNPILNVDQPYSEGFNTTVIWGPLMGIKLYAGLRFTLWK"
     gene            complement(3516..3962)
                     /locus_tag="P5293_07100"
     CDS             complement(3516..3962)
                     /locus_tag="P5293_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49702.1"
                     /translation="MKKSFQTLITSVLLSFYVIASLGIGVHECSRNGSINIRLFAYSR
                     SCNAIHPVCNCFHHHHDGIPEHQHHSSNCCHTTIYQLSQDYDIVSFNNAAVSASIDSN
                     SHVYIISNFEDSAINNSSALFCESAYTPPPLPIDINPYSFCSQWRL"
     gene            complement(4349..>4847)
                     /locus_tag="P5293_07105"
     CDS             complement(4349..>4847)
                     /locus_tag="P5293_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRR49703.1"
                     /translation="SDYDLLKSSLRKWEQDYLLISPLNGKVSFTKYWQENQNVSAGEV
                     VFTVLPEQKASISGKIYLPLSGAGKVRAGQKVNIKFDNYPYMEFGMVEVRVRGLSSVP
                     TVVDDSRVYVVDVELPGRLITNYGKELPFSEEMMGVAEIITDEQSVLNRVIFPVKHIF
                     KSRML"
BASE COUNT         1557 a          987 c          792 g         1511 t
ORIGIN      
        1 aacaattctc cgtcatgaaa aaagtaaata atataaacat tgataaaata agtcttttca
       61 tagtagtata agtcagtaag aatatgatgt aataaccaat ggtttgtcct cattgctcaa
      121 caatgtgtac aatagagtat ctcgcagcac atcccggtag ttgtatttct tgccgtcaat
      181 ctttatagtg tcacaaatca gcctcacacc atggctgtaa tcaacatatc tccccaagtg
      241 gccgttatac acgggctgga tgggagtacc atccaatctg tgccagccgt aaattataac
      301 atgaccaggt cttaaagaat cttgcaattt tgaacttata acaatatctt ttttgctacc
      361 ggcaataaat accccaggtt tgtaacctgc agcctttatt tgagcaaata taactctaga
      421 atgatcataa aaaatatctg gggtctcatt tctgtctcct cttggaatat agttgaaagg
      481 ttcaactttt aatcttgact ttttatatat gatatcaact attttgggag ttgggagaga
      541 acagcctgaa gaatcggcaa ttttctgagc agttgcagga gtcatgggaa tgataaaggc
      601 agatttaccc tctcccacag tcagataatc cggtttaaca aaaagagtaa ccctgtgagt
      661 tttatgattt gcatccctgt ctttataaga tatttttaca aaacctttca tataatcggg
      721 aacatttcct gacattattt cgtcgtatat cagtttttcc ctctcttcca aaggcaaagt
      781 tctcattttc tcataaaaag gagaatcacc tttattcacc tgcgaacaga gtgaattaaa
      841 ggtgattgtc aataacaaaa ctagcagaat ctctcttctc attacaagct actttgtaac
      901 ctcgatctcc tttgcagtgt aaccgagttt ctcaatagcc tttgcaagtt taaccttatc
      961 tgtcttggaa ggatcatact tcatccatac tgtatgattt tcaagattga ctttcaaatc
     1021 cttgactcct gcctcaaatg gcaatttatc ctcaagtttc ttcttgcagt tagggcaatc
     1081 aatatcaacc gaaaaggtta cttccatctc tttagctgcc tttttcccct gagcgtttgc
     1141 cccattcgag aaaaaaaaca gagatgcaac aagtatgagc gagattgatt taaataaatt
     1201 cttcatgact gtaatatttt gtaaaattaa taaaaaatta aaataattat ttccaaagag
     1261 taaatctcag accggcatat aatttaatcc ccataagtgg tccccaaatt actgttgtat
     1321 taaatccctc gctatacggc tggtctacat ttagaatggg atttttctgg cggtaattag
     1381 tcaagttctc accaccaata tatatgtcca gttttttaaa tttttttgta acctgggcaa
     1441 aaagaacaga atacacagga gaatactcct tgtccataaa gtttggtaat ctagcaggac
     1501 cattaagttg agccgtaaaa tcaaatgtcc acttattcat tcttgtggca tattgtaaat
     1561 taagcacccc cttatactta ctcatcagag gcctttcaac aagtccctga ttttcaagag
     1621 ttactttagc atctgtcagt ctataagtaa gcagaacagt aaatctttca acaggctgaa
     1681 ttgaaaaatc tgcctgataa gtatttgtat aggatcgtcc tgacaaattg taaagccaga
     1741 ttgtagagag atctctctct tgatccacaa tcaattgatg ttgaaaatcc gttctgaaat
     1801 actcaaaact tactgtcgca tttttgtcaa accctatcgg aagatatcct gtcagattca
     1861 ttccatatgt ccaagcttcc tccatatcag gatgttcttc catttttatc tttcgaccgg
     1921 ttgagagaat ccctaaatta tcagctattt gatttggcga tctgaatcca cgaccggctg
     1981 aagctctgag aacaagctta tcggtaaagt cgtacttaac atttatcctt ggagcaaaaa
     2041 gccatccatg aagattatta ttgtcaagtc taagccctgc taccacagtt atcttatctc
     2101 cattaacata ggtgtactca ccatatgccc ctgcagattg ctcttctctt cccacatttg
     2161 caccattaag atgctcgtca aaattgtcgt actgaccgct caaaccaatg atatatttat
     2221 gattgtcatt tatttggttc tgcaacatta aattgaaaaa aacagaattc tgagtagcat
     2281 cataactttt ataaccgaaa aaagagttca tatcatacat tgaatagtcc accacagcgg
     2341 caacattttt tgagttactc tcattcaagg gtatcccaag ttttgcatat ccgtttaaac
     2401 ccttgttttt aattttcgaa ccccatagat catctgtgac cttcgatcca tttttaaaat
     2461 cattcatacc ggcaatacgc tcgtcgctca ttgccttgat accaaatctc agttgcatac
     2521 cattggaagg ggcaaataac caacgatttg aaaaattgaa ttgagtagtt aaaggttcat
     2581 ccttaaatcc atcatcatta ccatcatgtt ccattggatc agttgaaaaa tgccctaata
     2641 tgacagtgct ccactttgga tttagctgca gagatgaagc tacatttgct tctgcacgca
     2701 gcatgttact caaaaagaga ttcacaaaaa gtggttgttc agttgtaggt tttctgtgct
     2761 ccatattaat ttgaccagta attgcctcaa gaccatttac aaccgaagaa ggtccttttg
     2821 caatttggat actttcaagc cattgtccgg gaatatacga aagaccaaat ggagctgcaa
     2881 ttccccgcat gacgggacga ttttcgtcaa gcatctgcgt atactgaccc gacaaaccaa
     2941 gaagtctgat ttgtcttgcc ccagtaattg catcagaata acccactgac acactggcag
     3001 aattttcaaa actctctgca agattacaac aggccatttt gcagagaccc gcagcagaaa
     3061 taacttctgt cttaacagga gttactttgg aaatatagtt tccctcttgc cttcctacta
     3121 ctctaatttc atcaaggctg ttgttctctt ctttaagaat aaaatctgcc ttgttttctc
     3181 cattttcaag tattagcgta tctctcgaat aaccgatata tgttgctatt agagagacct
     3241 ttcctgaatg ctcattcttg attgtaaatt taccatcctg atcagtctca agtgaaaatt
     3301 tgccttcgag ccaatatact gatgcgtaag gtaatggctc aatctcccca ctttcatgct
     3361 gtgccttgac aatgccatgt attgtctgcg aaaaagaaag tgttgttata gatattataa
     3421 ataaaaatgt aataattatt ctcttcatat taatttaaaa attgtaaagg attcctacaa
     3481 aaaggatatt ttctatctat ttgttgacag aatctctaca atctccactg agaacaaaat
     3541 gaatatggat ttatatcaat tggaagaggt ggaggagtat aagcactctc acaaaatagt
     3601 gctgagctat tatttatagc actatcctca aaattagaaa ttatataaac gtgcgaatta
     3661 gaatctattg aagcagaaac ggctgcatta ttaaagctaa cgatatcata atcctgagaa
     3721 agctgataaa tagttgtatg acagcagtta gatgaatggt gctgatgctc gggaatgccg
     3781 tcatgatgat ggtgaaaaca attacaaact gggtgaatgg cattacaact tcttgaatag
     3841 gcgaacaacc tgatgtttat agacccgttc ctggaacatt catgcacgcc gatgcccaga
     3901 ctagcaataa catagaaact aagcagcact gatgtaatta atgtttggaa cgacttcttc
     3961 atgcctgcaa aattaaaaaa aatccttcag tcagcaaggt tttcgtcata gaatttataa
     4021 aaaaccagga actgaatcgc ggcagtcaca agcacagtca aaataaaaac acaaataatg
     4081 catttgggca aaagataata atctggcaat ataaaatact tgtaaatcaa acaagtaagc
     4141 aatgtgcatt gcatcatttc gcgtaacaga gtaatgggag caaaaaattt actactatat
     4201 ctgaacaaag cagttactgt aaaatttaat acttcccatc tgcttttctt gacatttctg
     4261 aacaatttca ctctataaaa aagcgaagat ataagagaag tgcaacaagc agaaatagct
     4321 ccaacaaaca gtattaccaa agtaattctc ataacattct gcttttaaat atatgtttaa
     4381 cagggaatat aactctgtta agaacgcttt gctcatctgt aataatctcg gcaactccca
     4441 tcatctcttc tgaaaatgga agttctttgc cgtaatttgt aataagtctg ccaggtaatt
     4501 caacatcgac tacataaacg cgggaatcat ctacaactgt aggaactgaa gacaaacctc
     4561 ttactcttac ttcaaccata ccaaattcca tataaggata gttgtcaaat ttgatattta
     4621 ccttctggcc tgctctgact ttacctgcgc ctgacaaagg aagatatatc ttacctgaaa
     4681 ttgatgcttt ctgctcaggc aaaactgtaa aaaccacctc tccggcagaa acattctgat
     4741 tctcttgcca gtactttgtg aaagagactt tcccattgag aggagaaatt agcaggtaat
     4801 cctgttccca ttttcttaag gaacttttta acaaatcata atcactg
//
LOCUS       DAOTQL010000123         4835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_13871_length_4835_cov_4.173431, whole genome shotgun sequence.
ACCESSION   DAOTQL010000123 DAOTQL010000000
VERSION     DAOTQL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13871_length_4835_cov_4.173431"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1010
                     /locus_tag="P5293_07110"
     CDS             150..1010
                     /locus_tag="P5293_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HRR49704.1"
                     /translation="MKKSFVLTLSLSIIGLMIFLPSKLILAQKMYVIESKNLKTNDTV
                     LVFTPDVWSYDKSSNTPAMFLLHGWSGNWRNWSDKVDLQKLSNKWGFIIITPDGFYNS
                     WYLDNIDSTKMQWKKFFHKELYPLMVKEYRLSPEMTFISGLSMGGHGALSIFLDDISK
                     FRAAGSMSGVLNLPDSFGKDYQLPQVIGEYRKGSKNLRDNSVISRLDSAALNRAFKEG
                     DKILLATCGSQDFYAKSTLNFAKKCNKLKIPYILIMSPGNHNWTYWKFAVEQHLFIFS
                     RMASNAGIGY"
     gene            complement(1014..2720)
                     /locus_tag="P5293_07115"
     CDS             complement(1014..2720)
                     /locus_tag="P5293_07115"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRR49705.1"
                     /translation="MKLTKYIQKTLGAFIFLSLGFSSVVYGQKNEASLFKAMQDEMTR
                     TKNELKLPGALPTYFVGYTIAENKYISIISSLGTITYTKESPRERVNSVKLYVGDNNF
                     SSDYSYSGNGIRSNTLMTRDDSYDHIRYSFWQTSDLAYKFAVEVYNSKKSAVKTATIS
                     EEEKALPDMLPISYPEVSTPVIPEFNLNRKSYQDLANKLSKIFCNYSSVFNSSVGIDG
                     IETIYYYLTSEGTKIKEPVGYVSISVKGSIRNRKGQVIEDQKTIYAKSFDKLPAEDIL
                     IASVKDFAERLSSLIQAPNMDEYYLGPVLFEDEASAKIFADNLVSPSGILSFRHPYQV
                     VASVTRAENVSEAKNVKPLEERMGKKVIDSRLNVVNRTDLSDFGGFSLIGSYNYDGQG
                     VAPIKNLSLIENGILKNMLSSRVPTIKIKTSSGSMRFGVSPRSVINSLAPGVLIVSAP
                     SGEATTTLKTQLLNAAKEEGLDYAYIVRNFASGSGQYLYKVSVKDGSETLVTGAEISP
                     IQFIKLKRVLGVSKDDFVYNYLYKESIPTSIIAPKSVLIEDIEIVNKPLTIQKESPLI
                     GK"
     gene            complement(2757..4433)
                     /locus_tag="P5293_07120"
     CDS             complement(2757..4433)
                     /locus_tag="P5293_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRR49706.1"
                     /translation="MRKIFLLLASLLASLLLPAQDKMLDLLKGELNSQMATLQKGEYP
                     PYYMNYRVIDNFTRTVRSSMGATTGVEEEKQIIFVPQVRIGSPEFDNFHETQNGVLTN
                     RFAGPPTILLPSDLTNGLDAFREIIREEVNSRYKFAKKTYEAAKAKSGVKVENEDKSP
                     DFTPAKAEKYFEPALSADKIAFDTDLWQKKLNKYTALLAENRDVIGANATLNYKIWRK
                     YFVSTEGAEIAENRVYVVLSIKGTTMADDGMELPLNKSYMAFSLDELPSEAVIIKDLK
                     AMSTKLSELKVAPLVQPFTGPSLLSGSASGVFFHEIFGHRVEGQKMKSDSDGQTFKNM
                     VGEYVLPKNLSVYMDPTMKKYHGNPLNGYYIFDDQGVRGERVEVVKDGKLLNFLMTRT
                     GLNGFLKSNGHARAEFEYDPTSRQSNLIVETNDYKSDAELRKMLIDEAKQQGKEYGYF
                     FKAVTGGFTQTGRTGANSFNVTPLEVYRIYVDGRKDELVRGVDMIGTPLSMFSNIDAA
                     GGDFEIFTGTCGASSGNVPVTAISPIILVNKVELQKKAKPTVTPALLPRP"
     gene            complement(4503..>4835)
                     /locus_tag="P5293_07125"
     CDS             complement(4503..>4835)
                     /locus_tag="P5293_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49707.1"
                     /translation="VQMEKAAHKLNKKSPMMIDNETRLDSAVVFGDSLFQYNYTMVNF
                     IKDSIDTLGFRHYMKPKLLKFVRFNDDLKPYRDHKVTIEYVYNDKNGFLLGKFTFTPN
                     RYSESFEF"
BASE COUNT         1529 a          973 c          792 g         1541 t
ORIGIN      
        1 ttaaaattaa agcaactaaa aaaaggctta catagagagt attatcctta atatatgcaa
       61 tcagtctctt cattatttta aaataaaact atcagttgat aaaaataatt taaatttgat
      121 taaaaataac ctgatattca aataaaaaca tgaaaaagag ttttgttctt actttatcat
      181 taagtattat tggtctgatg atatttttac cttcaaaatt gattcttgcc cagaaaatgt
      241 atgttataga atcaaaaaac cttaaaacca atgatactgt acttgtcttc actcccgacg
      301 tatggagtta tgacaaatcc agtaacactc ccgcaatgtt tcttctgcat ggttggagcg
      361 gaaactggag aaactggagc gacaaagttg atttacaaaa gttaagcaat aaatggggtt
      421 tcattattat taccccggac ggattctaca acagctggta tctcgacaat atcgacagca
      481 ccaagatgca gtggaagaag ttttttcaca aagaattata ccccctgatg gtcaaagaat
      541 atagattgtc accagaaatg acattcataa gcggtctgag tatgggtgga cacggtgcac
      601 taagcatttt tctcgatgat atatcaaaat ttcgtgcagc aggaagtatg tctggtgtat
      661 tgaatcttcc tgactcattc gggaaagatt atcaacttcc tcaagtaatt ggtgaatatc
      721 ggaaaggctc aaaaaacctg agagacaact cagtaataag tcgcctcgac agtgcggcac
      781 tcaacagagc atttaaagaa ggcgataaaa tcctgctagc aacatgcggc tcgcaggatt
      841 tttacgccaa aagcactcta aactttgcaa agaaatgcaa caaactcaaa ataccataca
      901 ttctaataat gagtcccgga aatcataact ggacatattg gaagtttgca gtagagcaac
      961 accttttcat attttccaga atggcctcaa atgcaggaat tggttattaa aaattatttc
     1021 cctattaatg gagactcttt ctgaatagtc aaaggcttgt taactatttc tatgtcctca
     1081 atcaaaacag attttggtgc aattatagaa gtaggaattg attctttata aagataattg
     1141 taaacaaaat catcctttga tactccaagt actctcttta acttaatgaa ttgtattgga
     1201 gagatttcag cacctgttac aagagtctcg gaaccatctt taacagatac tttgtacaga
     1261 tattgaccag atccactggc aaaattcctt acaatatatg cataatcaag tccctcttcc
     1321 tttgcagcat tcaaaagctg agttttaaga gtagtggttg cctctccact gggagctgaa
     1381 actatcaaaa ccccgggagc taaggagtta atgacacttc ttggactaac tccaaacctc
     1441 atgctaccag aggaagtttt aatttttata gttggaactc gtgagcttag catattcttc
     1501 aatattccat tctcaatcaa cgacagattt tttattggcg caacaccttg cccgtcatag
     1561 ttataactgc ctatcaaaga aaatccacca aaatcagaaa gatctgtcct attaactaca
     1621 ttcaatctac tgtcaataac ctttttcccc attctctctt ccaacggttt cacatttttt
     1681 gcttcactca cattctctgc cctcgtaaca gaagcaacta cttgatatgg atgtctgaat
     1741 gaaagaatgc cggaaggcga aacaagatta tccgcaaaaa tttttgccga tgcctcatct
     1801 tcaaaaagca caggaccaag ataatactca tccatattcg gagcttgaat caaagatgaa
     1861 agtctttcgg caaaatcttt caccgaagca ataagaatat cttcagctgg tagcttatca
     1921 aagctttttg catagatggt tttttgatcc tctataactt gtccttttct attcctaata
     1981 ctgcctttaa cagatattga cacataacct acaggctcct taatttttgt cccctcagat
     2041 gttaaatagt aatatattgt ttcaattcca tcaatcccta cagatgaatt aaaaacagaa
     2101 gagtaattac aaaaaatctt tgaaagttta tttgcaagat cctgataact ctttctattc
     2161 aggttaaatt caggaataac aggtgttgaa acctcagggt aacttattgg caacatgtcg
     2221 ggtaaagctt tctcctcttc tgaaattgta gcagttttaa cagcactttt ctttgaattg
     2281 tagacttcaa cagcaaattt atatgccaag tccgatgttt gccagaagct atatcttata
     2341 tgatcataac tgtcatctct tgtcatgagt gtatttgatc tgatgccatt accggagtat
     2401 gagtaatcac tcgaaaagtt attgtcgcca acataaagtt taacagaatt aactctctct
     2461 ctaggactct cttttgtata tgttattgta ccgagggaac tgattatgga aatatactta
     2521 ttttcagcta ttgtgtaccc aacaaaatat gtcggtagag ctccgggaag cttcaattca
     2581 tttttagtac gcgtcatctc atcctgcatt gccttaaaaa gagacgcttc atttttctgt
     2641 ccataaacaa cagatgaaaa accaagagag agaaatataa aagctcctaa ggttttctga
     2701 atatattttg tcaatttcat ttcacaaaaa tttaattatt aaacaacatt atgatattac
     2761 gggcgaggaa gaagtgccgg agttacagtc ggctttgcct ttttctgaag ctctaccttg
     2821 ttaacaagaa taataggaga tattgcagtg actggaacat tacctgaaga ggctccacat
     2881 gttcctgtaa atatctcaaa atcaccacct gcagcatcga tattagaaaa catggagagt
     2941 ggtgtaccta tcatatctac acctctaacc agctcatcct tcctgccatc aacatatatt
     3001 ctgtatacct caagaggagt aacgttaaat gaattagcac ctgttcttcc tgtttgtgta
     3061 aagccgcctg ttactgcttt gaaaaagtaa ccatattcct taccctgttg cttagcctca
     3121 tcaatcaaca tcttgcgaag ttctgcatcg cttttgtaat catttgtttc aactataaga
     3181 tttgattgtc tggatgtcgg atcatattca aattcggctc gtgcatgacc gtttgacttt
     3241 agaaatccgt tcaacccagt acgtgtcatc aaaaaattta gcaatttacc atctttgaca
     3301 acctcaactc tttctcctct tacaccctga tcatcaaaaa tatagtagcc atttagagga
     3361 ttgccatgat attttttcat tgtcggatcc atatagacac taaggttttt aggcaaaaca
     3421 tattctccaa ccatattttt gaatgtttgg ccatctgaat cactcttcat cttctgtcct
     3481 tcaaccctgt gcccgaaaat ttcatgaaag aaaactccac ttgcagaacc agaaagaagc
     3541 gatgggccag taaatggctg taccagtgga gcaaccttaa gttccgacag ctttgtcgac
     3601 atagccttga gatccttaat tataacagct tcagatggaa gttcatccaa agaaaaagcc
     3661 atatagctct tgtttaaagg aagttccata ccatcgtcag ccattgtagt acctttaata
     3721 ctaaggacca catatactct gttctcagca atttctgcac cttctgttga aacaaaatat
     3781 tttctccaaa tcttataatt gagagttgca tttgctccaa ttacatctct attctcagcc
     3841 aaaagagctg tatatttgtt cagcttcttc tgccataaat ctgtatcaaa ggctatcttg
     3901 tcggcactta gtgcaggttc aaaatatttt tcagccttgg ccggagtaaa atcaggagac
     3961 ttgtcttcgt tttcaacttt cactccactc tttgccttag ctgcctcata ggtcttttta
     4021 gcaaatttgt atcgactgtt tacctcctca cggataattt ctctaaatgc atcaagtcca
     4081 tttgtcaaat cagatggaag taaaattgta ggagggcctg caaatctgtt tgtcaaaacc
     4141 ccattttgtg tttcatggaa attgtcgaat tcagggcttc ctattcttac ctgaggtacg
     4201 aaaataatct gtttctcctc ttctacaccg gttgtagcgc ccatagagga tcttacagtc
     4261 ctggtaaagt tgtcaattac cctgtaattc atgtaatagg gaggatattc tcccttctga
     4321 agcgtagcca tctggctgtt taattcacct tttaacaggt caagcatctt atcctgagca
     4381 ggaagcaaga gcgaggccag caaagatgcc agcaacaaaa atatttttct catcgtattt
     4441 tggttaatat aaatttttct tttgacgaaa gtaatctaaa aaggtttaat cactcataag
     4501 gttcaaaatt caaaagattc tgaatatcta tttggagtga aagtgaattt accaagcaaa
     4561 aaaccatttt tatcattata aacgtactca attgtcacct tgtggtctct atagggtttc
     4621 aaatcatcgt taaatcttac aaatttcaaa agtttcggtt tcatgtaatg tctaaaaccg
     4681 agagtatcaa tagagtcttt aataaaattg accattgtat aattgtactg aaataaggaa
     4741 tcgccaaata ccacagcact gtcaagcctt gtttcattat caatcatcat tggagatttc
     4801 ttgtttaatt tatgtgcagc tttctccatc tgcac
//
LOCUS       DAOTQL010000124         4741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_14198_length_4741_cov_4.368545, whole genome shotgun sequence.
ACCESSION   DAOTQL010000124 DAOTQL010000000
VERSION     DAOTQL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14198_length_4741_cov_4.368545"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="P5293_07130"
     CDS             complement(<1..1306)
                     /locus_tag="P5293_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRR49708.1"
                     /translation="MSKNPLRRLAGETVIYGLSSILARFINFVFVPIYTRVLSTSQYG
                     LATEVLAYIAMLQVILTLGLETGCFRYANKHNKPDEVFSNALLTVGSLSLLVLAGLTI
                     GSGSISRLMGYNGFENIIIYVGAILAIDNFTAILFAKLRFLHKAFKFAIIKTIKILSE
                     TAFNLILFFAVPGYLSKHPGSFLLNFISPTPDFSYIIFAIFLSCIILLVIMLPDILKI
                     RIKFNVPLWKEMMLYSLPLMIAGLPGILNDFIDRPLFRMLSPKGLEWSSELGIFQAGA
                     KLATIMMLFVQMFRFAAEPFFFSKDESEEVKKKQYANVMEHFTAFSMLIFLGLTLYMD
                     VIGLIIGKNFRQGVDIVPIMLMAYVILGMNFNVSMWYKLSGKTNYGILITAAGLLVTL
                     LINIIFMPLYSYHAAAWGHLVSYLVMMLISIMLGNRYYPIPYK"
     gene            complement(1309..3627)
                     /locus_tag="P5293_07135"
     CDS             complement(1309..3627)
                     /locus_tag="P5293_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRR49709.1"
                     /translation="MQIKKIVIIILLSVSAIAASAQSVGLVLSGGGAKGLSHVGAIKA
                     LEDNGIPIDYVAGTSMGAIVGALYSIGLTPDEMIKLFKSKEFESWYNGEQEPMFASYI
                     YRRDPTPEMVKLSASINSEGKKLSINFPSSIIAPYPMDLAVMQIFASPAAAAKYNFDN
                     LMVPYRCVGADVSRKSPVAFSKGDLGSAVRASMTFPLVFQPIMIDSVLYFDGGLYNNF
                     PWDVMVKDFNPDYLIGIKCASQFASVPKPDDVLSQLESMLTVETNYNIPEDKGILLDL
                     KFEDVNLLDFDKIDQLVQAGYFNMMKLMPEIKENVQRRRSEEELLQKRLDFRTRCVPL
                     IFEKVIIKDSLRYNAQDFIDKTFRENRTRYFDFEQLKRGYYRIIASGNVKKFFPTAKL
                     SKDSVFNVNLQVSEAPPIKIAVGGNISSSSLNQGYLGIEYRKFTGMPWRVAADVNFGR
                     FYAGFNGYFRQDFSVRPFAFYELQFVSHRFDYFGANQTNFFSHRVPSSNAQEGETFVT
                     ASIATPVSLYENLLAKLTFTAGENDYEYYHVDNFTYYDIPDKTELKYLSPSFYLEKNT
                     TNYRLYPTNGSIYKISLRYTHLREDYRPGSTIIDAQPISKKMHNSMSIRAYFESYIPL
                     SKKFTLGWIADVTVSNRTALGDRTSSLLYLPAFQPTPHSKTLFLSEYRAQSFAGAALM
                     PIYNITKSLSLYLTGAYFQPYKYILQTSDGSTKFSDRFPKGKFLGEIAGVWQSPIGPV
                     TLSATYYEKSDIKWFPQLNIGFLIYKPKALAY"
     gene            3819..4385
                     /locus_tag="P5293_07140"
     CDS             3819..4385
                     /locus_tag="P5293_07140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR49710.1"
                     /translation="MTAALAFDPSTLGEEPVFDINTTLGTIRVKLYKETPKHRDNFVK
                     LASERFYDDVLFHRVINGFMIQAGDPLTKDTLNADKFGTGGPGYTIPAEFNPKFKHKK
                     GALAAARRGDASNPEKESSGSQFYIVQDPKACSQLDGQYTIFGETVSGLDVIDKIAAT
                     PTDKRDRPLTDIKILSIVPVIENKPAAK"
     gene            complement(4386..>4741)
                     /locus_tag="P5293_07145"
     CDS             complement(4386..>4741)
                     /locus_tag="P5293_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR49711.1"
                     /translation="SGGMQKRVGIARAIALNPKYLFCDEPNSGLDPTTAILIDQLIYE
                     ITKEYNITTIVNTHDMNSVMGIGDQVGFIHKGELVWEGDKNNILSSDNEKLNDFVFAS
                     KMAKRMKELMTEKDM"
BASE COUNT         1523 a          866 c          914 g         1438 t
ORIGIN      
        1 atttataagg aatgggataa tatctgtttc caagcattat acttattaac atcattacta
       61 agtagcttac aagatgtccc catgctgcag catggtagga atataggggc ataaatataa
      121 tgtttatcag aagggttacc agtagacctg cggcagtaat aagaatgcca taatttgttt
      181 ttcctgaaag tttataccac atagaaacat taaaattcat tccaagaata acataagcca
      241 taagcattat ggggacaata tcaactcctt gacggaaatt tttgcctatg attagtccaa
      301 tcacatccat atatagtgta agtccgagaa atataagcat cgagaaagca gtgaagtgct
      361 ccatgacatt tgcatattgc ttctttttaa cctcttcaga ttcatctttt gagaaaaaga
      421 aaggttctgc cgcaaaccgg aacatctgga cgaaaagcat cataattgta gccaactttg
      481 ctcctgcctg gaatatccca agttcagacg accattccag cccttttggg gagagcattc
      541 tgaacaaagg tctgtcaatg aagtcgttta aaattccagg gagtcctgca atcattagag
      601 gaagagaata aagcatcatc tctttccaaa gaggaacatt gaatttgatt ctgattttca
      661 aaatatcagg caacattatg acaagcagaa taatacaact cagaaaaatt gcaaagataa
      721 tgtaagagaa atcaggggta ggagagataa aatttaacag gaaacttccg ggatgttttg
      781 acagatagcc tgggacagca aaaaacagta taaggttaaa agcggtttct gatagaattt
      841 tgatagtctt gattattgcg aatttaaatg ctttgtgcaa aaaacgcagt tttgcaaaga
      901 gaatagcggt aaaattatca attgcaagga ttgctccaac atagataata atattttcaa
      961 atccattgta tcccatcagg cgggagatgg aaccggagcc aattgtcaaa cctgcaagaa
     1021 ccagcagaga cagagagccc actgtaagta gagcattgga gaacacctca tcaggtttat
     1081 tatgcttgtt tgcatatctg aagcatccag tttcaagccc aagagtaaga ataacctgca
     1141 gcatagctat atatgccaac acttcagtag caagtccgta ttgtgaagtt gaaagtactc
     1201 tggtataaat agggacaaag acaaagttga taaatctggc aaggatagaa cttagtccat
     1261 atataacagt ttcaccggca agccgtctaa gaggattttt actcattact aataagcaag
     1321 tgctttaggt ttatatatta gaaatcctat attaagctgt ggaaaccatt taatgtcgga
     1381 tttttcataa tatgtggctg atagtgttac aggtcctatt ggtgattgcc atactcctgc
     1441 aatttctcct aagaactttc cttttggaaa gcggtccgaa aatttggtgc taccatcgga
     1501 agtttggaga atatatttgt atggctggaa atatgctcct gttaaataga gcgaaagact
     1561 tttagtaatg ttgtagattg gcatcaaggc agcgcctgca aaagattgag cacggtattc
     1621 gcttagaaat agagttttac tgtgaggtgt cggttgaaat gcaggaagat atagtagaga
     1681 cgatgttcta tctccaagtg cagtcctgtt ggacacggta acatcagcaa tccatccgag
     1741 agtaaatttt ttacttaatg ggatataaga ttcaaagtac gcccttatac tcatagagtt
     1801 atgcattttt tttgatatcg gttgagcatc aataatggta ctccctggtc ggtaatcttc
     1861 tctaagatgt gtatatctga gagaaatctt atagatgctt ccatttgtgg gatacagtct
     1921 ataatttgtt gtgttttttt ctaagtaaaa ggatggagaa aggtatttca gctcagtctt
     1981 gtccggtata tcgtaatatg taaagttatc tacatgataa tattcataat cattttcacc
     2041 tgctgtaaat gtcagctttg ccaaaagatt ttcatagaga ctcacaggag ttgcaatgct
     2101 tgcggtaacg aaagtttcgc cttcttgagc attacttgaa ggaactctgt gggagaagaa
     2161 attagtctga ttcgctccaa aatagtcaaa acggtgtgat acaaactgta attcataaaa
     2221 agcaaatggt cttacagaaa aatcctgtct gaagtaacca ttaaatccgg cataaaatct
     2281 tccaaaatta acatctgctg ctactctcca cggcattccc gtgaattttc ggtactcaat
     2341 tccaagataa ccctggttga gagatgaaga tgaaatattg cctccaacag ctatttttat
     2401 tggaggagct tcggatacct gaaggttgac attaaagacg gaatctttgc tgagtttggc
     2461 tgtaggaaag aatttcttta cgtttccact tgcaattatt ctgtagtatc ctcttttgag
     2521 ttgctcaaaa tcaaagtacc ttgttctgtt ttctctgaat gttttgtcga taaaatcttg
     2581 ggcgttgtat ctcagagagt ctttaattat aactttttca aatatcagag ggacacatct
     2641 ggttctgaaa tcaaggcgtt tctgcagcag ttcttcttca ctccttcttc tttggacatt
     2701 ttctttaatt tcaggcatta atttcatcat attaaaataa ccggcctgga caagctgatc
     2761 tattttgtca aaatcgagga gatttacgtc ttcaaatttc aaatcaagta atatgccctt
     2821 atcttcagga atattatagt ttgtttcgac tgtaagcatt gattccaatt gcgaaagcac
     2881 atcgtcgggt ttgggaacac tggcaaactg agaagcacat ttaataccaa ttaaataatc
     2941 cggattaaaa tccttaacca ttacatccca aggaaaatta ttataaagtc caccatcaaa
     3001 atagagcaca gaatcgatca taattggctg aaaaactagt gggaaagtca tagatgccct
     3061 gactgccgag cctaagtcac ctttgctgaa tgccacaggg gacttacgag aaacatctgc
     3121 acctacacat cggtaaggaa ccattaggtt gtcaaaattg tattttgcag cagcagcagg
     3181 agaggcaaat atttgcatta cagccaggtc catcggatag ggagctataa tactcgaagg
     3241 gaagttaata gaaagttttt tcccctcact gttaatactg gcagaaagtt ttaccatttc
     3301 aggagtagga tctcttctat atatgtaaga agcaaacatt ggctcctgtt ctccgttata
     3361 ccaggattca aattctttgg acttaaacag tttgatcatt tcatctggtg taagccctat
     3421 tgaatataga gctccaacta tcgcccccat tgatgttcct gctacataat ctatagggat
     3481 gccgttatcc tctaaagctt tgattgcacc aacatgagaa agtcccttag caccaccacc
     3541 acttaatacc aatcccacac tctgagcaga agcagcgata gctgatactg acagaagtat
     3601 tattattaca atttttttaa tttgcataat cttacctttt aaggtatttt tgtagagaaa
     3661 tacaaaggta taaaaaatag tacaataatt tagataaaat tatgaagaca aacattttca
     3721 atacgctgat aattatttcg ttagtacttt tttcatgcaa aggtaacaag ggtacccagc
     3781 aacaggaaaa taaattagaa acaaaagaca cagtaaaaat gactgcagcg cttgcatttg
     3841 atccctcaac tttaggggaa gaacctgttt ttgacattaa tactacatta gggacaatca
     3901 gagtcaaact ttacaaagag acacctaagc atagagacaa ttttgtaaaa cttgcttccg
     3961 aacgtttcta tgacgatgtt ctgtttcaca gagttattaa tggttttatg attcaagctg
     4021 gtgacccgtt gacaaaagat actcttaatg ccgataaatt tggtacagga gggccaggct
     4081 atacaattcc ggcagagttc aatcctaagt ttaaacataa aaagggagca ctggcagctg
     4141 ctcgaagggg agacgcgagc aatcccgaga aggaatcttc aggttctcag ttctatatcg
     4201 ttcaggatcc aaaggcgtgc agccaacttg atggacaata tacaatcttt ggtgaaacag
     4261 ttagtggttt ggatgttatt gataaaattg cagcaactcc cacagacaaa agagacaggc
     4321 ctcttactga cattaaaata ttgagcattg tgccggttat agaaaacaaa ccagctgcca
     4381 aataattaca tatctttctc tgtcatcagt tctttcattc tttttgccat tttggaggcg
     4441 aagacaaaat cgtttaattt ttcgttgtcg gatgaaagta tattgttttt gtcaccttcc
     4501 cagacaagtt ctcctttatg gataaatcca acctgatcgc caattcccat aaccgagttc
     4561 atatcatgtg tattgacaat tgttgttatg ttgtactctt tggttatctc atatataagc
     4621 tggtctatta aaatggcagt tgttggatca agaccagaat tgggttcatc gcaaaacaga
     4681 tatttaggat taagagctat ggctctcgct attccgactc ttttttgcat acctccactt
     4741 a
//
LOCUS       DAOTQL010000125         4627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_14636_length_4627_cov_4.746063, whole genome shotgun sequence.
ACCESSION   DAOTQL010000125 DAOTQL010000000
VERSION     DAOTQL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14636_length_4627_cov_4.746063"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..848
                     /gene="cdaA"
                     /locus_tag="P5293_07150"
     CDS             66..848
                     /gene="cdaA"
                     /locus_tag="P5293_07150"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRR49712.1"
                     /translation="MLDFIKIDLIDIIDIFLVGLLIYQIYKLIRGTAALNIFIAIIVI
                     YFIWLIVKALHMELLGAIMGQVIGVGVIALIVLFQQEIRRFLLMIGTRYMNSQRKLKL
                     ANMLFGVKGRSVSLNLLEEVTLACRRMSENRTGALIVLANNSSLEFIIETGDRLDAII
                     NRRLIENIFFKNAPLHDGAMVIAYDKIVAARCTLPISENPHILPSYGMRHRAAVGVTE
                     ITDASVIVVSEETGDISFVNDGEISKMNSINELRLAIENAYK"
     gene            851..3349
                     /locus_tag="P5293_07155"
     CDS             851..3349
                     /locus_tag="P5293_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRR49713.1"
                     /translation="MDERLEQKLGFDRIRSMTESRCFTSKARERANGNLFSISLADIE
                     EELSKSDEMRLIIMFESGFPDSGYVDTYPFLKQLEHPSYILDLNSLVRLRSSLETIRQ
                     IVIFFRNVKEGKYPRLRKMADSIVSFPEVNRRIDAIIDKFGDIRDNASSELQSIRKSL
                     KDKEGQISRRIQAILKNAQSEGLVEEDVSISVRDGRVLIPVSASNKRKIPGIIYDESA
                     SGKTAFVEPLEIVELNNQVRELHFAEQREIVRILTEFSDFLRPYLPELLKSAEFLSEI
                     DFLRAKGLLAIQMQAGKPIISSEQLVVLNKGRHPILESALKKEGKEIVPLSFSLSKEK
                     HILLISGPNAGGKSVCLKTVGLLQYMFQCGFLVPASEVSEFAVFNEIFIDIGDEQSIE
                     NDLSTYSSHLKNMRDILLHADNHSLVLIDEFGSGTEPAAGGAIAEAVLAEIEKKGVFG
                     VITTHYSNLKYYASGSKGVINGGMQFDVQNIKPLFKLEIGTPGSSFAFEIARKMGLPE
                     EVVKSAEERVGNDFVDLEKHLKKIAKNKRQLDEKLARIKNTDRVLESITDKYQKELED
                     IQSLKKSIIENAKKEAASIIAEANKKVEQTIKEIKESQAEKEKTKILRKELVEFKNEI
                     QSENPSEEDIRIAKKMDQLLRRKERQAEKRENRNRKNTVVIQKDPILPKTESAGLKIG
                     DKVRVDEGDVVGEIIRIEGEEVRVAIGSIISRIAKKRLSKISGKEFANFNNSSVKSFV
                     VEGSSISERKLNFKPSIDVRGQRVEEAIDNVTRFVDDALMVGVNEITILHGKGNGILR
                     EELRKYLKTMGVVESLRDEDIQFGGSGITIVKLI"
     gene            3374..3775
                     /locus_tag="P5293_07160"
     CDS             3374..3775
                     /locus_tag="P5293_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49714.1"
                     /translation="MKKISIIFMAIAFLFLASCTGREKEISQKTLSFLDAYFKTDFSL
                     AGTFCTNAMALELKDKFKSIDSLDPVIKEMLKNHSRLIQTQILAVERFKRGDSARVSY
                     RVIMPEGKQYFDKSISLVMEDKEWRISKLGK"
     gene            3784..4587
                     /locus_tag="P5293_07165"
     CDS             3784..4587
                     /locus_tag="P5293_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRR49715.1"
                     /translation="MRLFDSHTHCEFSPDSRIKMKDAIERAFQTGLRGITFTDHLDID
                     TPTQDRSFMFNPAEQQVAIDSLKKREGIEVLKGIEVGLQPHTLEKVADYLEGSFFDQV
                     IVSVHFVDGVDPYHQPYYDGKDLRMAYGRYLDIIFYCITKYKDFDILGHFDYIARYAP
                     YCQKSMLYADFPVQFDRILSTLASQGKALEINTGTYREKNGSKPVLDSCVLKRFKELG
                     GKYVSLGSDAHDLERIGENFAYYSHLIKDCGFEYITHFNKREPELIKIE"
BASE COUNT         1566 a          716 c         1008 g         1337 t
ORIGIN      
        1 gtatcatttg ccacattggc ttttgcagaa taataactaa atttgtagct gtaagctgaa
       61 gttgtatgct cgatttcatt aagatagatc taattgacat aattgatata tttttggtgg
      121 gtttattgat ctatcaaatc tacaagctta tcaggggcac tgctgctctt aatattttca
      181 ttgcaattat tgttatttat tttatatggc tcattgtcaa agctcttcat atggaattac
      241 tcggggcgat aatgggacag gttataggag taggagttat tgccttgatt gtacttttcc
      301 agcaggaaat cagacgtttt ttgctgatga ttggtacgag gtacatgaat tctcagcgta
      361 aactaaaact ggcaaatatg ctttttggtg tcaagggaag aagtgtttct ctcaatttat
      421 tagaggaagt aactctggct tgcagaagaa tgtctgagaa tagaacaggc gccctgattg
      481 ttcttgccaa caattcttct cttgaattta ttattgaaac cggagacagg cttgatgcta
      541 ttattaacag aaggcttatt gaaaacattt tttttaaaaa tgctcctctt catgacgggg
      601 ctatggtcat tgcctatgat aaaatagttg ctgcaagatg cactctgcca atatctgaga
      661 acccacacat tcttccctca tatggcatga gacatagagc tgcagtaggt gttaccgaaa
      721 ttactgacgc aagcgttatt gtagtttctg aagaaacagg cgatatatca tttgtcaacg
      781 atggagagat aagtaagatg aacagcatca acgagttgag acttgcaata gaaaacgctt
      841 acaagtaaat atggacgaaa gacttgaaca aaaactgggt tttgatcgta ttcgttcaat
      901 gactgaatca aggtgtttca cttcaaaggc aagagagcgc gctaacggca accttttcag
      961 catatctctt gcagatatag aggaagagtt gtctaaaagc gacgagatga gacttattat
     1021 tatgttcgaa tccggttttc cggattccgg atatgtagat acctatcctt ttcttaagca
     1081 gttagagcat ccatcttata tacttgattt aaactcactg gtcagactta gaagttcact
     1141 cgaaaccata agacagattg tcattttttt tcggaacgta aaagagggta aatatccaag
     1201 attgagaaaa atggctgatt ctattgtttc ttttccggaa gttaacagaa ggattgacgc
     1261 aataatagac aagtttggag atattagaga taacgcttct tctgaacttc aatccataag
     1321 aaaaagtctc aaagacaaag agggacagat ttcaagaagg atacaggcta tacttaagaa
     1381 tgcccagtct gaaggtcttg ttgaagaaga tgtgtcaatt tcagtgagag acggaagagt
     1441 tctgatacct gtatctgcat ccaacaaaag gaaaatacca ggtattatat atgatgaatc
     1501 tgcttcagga aagacagctt ttgtagaacc tcttgagata gttgaattaa ataaccaagt
     1561 cagagaatta cattttgcag agcagagaga aatagtacga attcttactg aattttccga
     1621 ttttcttcgt ccttatttac cggaattact aaagtcggca gaatttttgt ctgaaattga
     1681 ttttttgagg gcaaagggac ttttggctat tcaaatgcag gccgggaaac caataatatc
     1741 ttcagaacag ttagttgttc ttaataaggg gagacatcca attcttgaat cagcattgaa
     1801 aaaggagggt aaagaaatag taccactatc tttttcacta agcaaggaaa aacatatact
     1861 tctaatctct ggtccaaatg cgggagggaa atctgtctgt ctgaaaactg ttggtttgct
     1921 gcagtatatg tttcaatgcg ggtttcttgt tcctgcttct gaagtgtctg aatttgcggt
     1981 atttaatgaa atttttattg atataggaga cgaacagtcc attgagaatg atttaagtac
     2041 atatagttca catcttaaaa atatgagaga catccttttg catgcagata atcactcact
     2101 tgtacttata gacgaatttg gttccggcac cgaacctgcg gcgggaggcg ctatagcaga
     2161 ggctgtgctt gcagaaattg aaaagaaggg tgtatttgga gtaattacaa cacactacag
     2221 taatctgaaa tattatgcaa gtggaagtaa gggagttatc aacggtggaa tgcagtttga
     2281 cgtacagaac ataaaaccac tttttaaact ggagattggc actcctggca gttcttttgc
     2341 atttgagata gcaagaaaaa tgggtcttcc ggaggaagtt gtcaaatctg cagaagagag
     2401 agttggtaat gattttgttg accttgaaaa acatcttaaa aaaatcgcaa aaaataaaag
     2461 gcagttagat gagaaacttg caaggataaa aaacaccgac agagttttag aaagtataac
     2521 agataaatat caaaaagaac tggaggatat acagtctctt aaaaaatcaa ttattgaaaa
     2581 tgccaaaaag gaagcagcat caataattgc agaagcaaac aaaaaagttg agcagactat
     2641 taaagagata aaggagagtc aggcggagaa agagaaaact aaaattctca ggaaggaact
     2701 tgttgagttc aaaaatgaga tacagtccga aaatcctagt gaggaagaca tcagaattgc
     2761 gaaaaaaatg gatcaacttt tgagaaggaa agaaagacaa gccgaaaaaa gagagaatag
     2821 aaaccgaaaa aatactgttg taattcaaaa agatccaata ttgccgaaaa cagaatcagc
     2881 aggattaaaa attggcgata aagtcagggt tgatgaggga gacgtggttg gcgagattat
     2941 caggattgaa ggtgaggaag tgagagtggc cataggaagc attataagta gaattgcaaa
     3001 gaaacgcctc agcaaaatat ccggcaaaga atttgcaaat ttcaacaaca gttctgttaa
     3061 gagttttgtt gttgaaggga gttcgatatc agaaagaaaa ttgaacttca agccttcgat
     3121 cgatgttaga ggccaaaggg tagaagaggc aatagacaat gtaacaaggt ttgtagacga
     3181 tgcccttatg gtaggtgtaa atgaaataac gattcttcac ggcaaaggga atggtatttt
     3241 gagagaggaa ctgagaaagt atcttaaaac aatgggagtt gtagagagtc tgagagatga
     3301 agatattcaa tttggtggtt caggaatcac aatcgtaaaa cttatataat tttgtaaaat
     3361 taactcaata gatatgaaaa agatttcgat aatttttatg gcaattgcct ttttatttct
     3421 tgcttcatgt acaggcaggg aaaaggaaat atcccaaaaa actctttcat ttcttgatgc
     3481 ttacttcaaa accgactttt ctcttgcagg aactttttgt accaatgcca tggcacttga
     3541 actcaaggac aagtttaaga gtattgattc gcttgatcct gtcattaaag agatgttgaa
     3601 aaatcactcc cgcctgatac agacccaaat actggcagtt gaaaggttca agagaggaga
     3661 ctcggctaga gtcagttatc gtgtgataat gcctgaagga aaacaatatt ttgacaaatc
     3721 aatttcactt gttatggaag acaaggagtg gagaatatct aaacttggca aataggagaa
     3781 aaagtgcggc tgtttgattc tcatacacat tgcgaatttt ctcccgattc gcgcataaag
     3841 atgaaagatg ctatcgaaag agcttttcag acggggctta gaggtattac attcacagac
     3901 catcttgata ttgacactcc gactcaggac aggagcttca tgttcaatcc tgcggagcaa
     3961 caggtggcta ttgacagttt gaagaaaaga gaaggcatag aagttttgaa gggaattgaa
     4021 gtcggacttc agccacacac tctggaaaag gttgcagact atctggaggg gtcgtttttt
     4081 gatcaagtaa tcgtatctgt ccattttgtt gatggagtag acccatacca ccaaccatat
     4141 tatgatggta aggatttgag aatggcatat gggagatatc ttgacattat tttttactgt
     4201 ataacaaagt acaaagattt tgacattctt ggtcacttcg attatattgc aagatatgca
     4261 ccttattgtc aaaagtcaat gctttatgcc gattttccgg tgcagtttga tagaatactt
     4321 tcaacacttg cttcacaagg caaagcacta gaaataaata cggggacata cagagaaaaa
     4381 aacggcagca aaccggtact tgattcttgc gttttaaaaa gatttaaaga gttgggagga
     4441 aaatatgttt ctcttggttc cgatgctcac gatctggaaa ggataggtga aaattttgcc
     4501 tattatagtc acttgatcaa agattgcggt tttgagtata taactcattt caataagagg
     4561 gaaccggaac tcataaagat tgaataatca tctcgttatg acagggatgt cttctatttc
     4621 ttttttt
//
LOCUS       DAOTQL010000126         4609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_14703_length_4609_cov_4.549846, whole genome shotgun sequence.
ACCESSION   DAOTQL010000126 DAOTQL010000000
VERSION     DAOTQL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14703_length_4609_cov_4.549846"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5293_07170"
     CDS             complement(<1..579)
                     /locus_tag="P5293_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRR49716.1"
                     /translation="MDNSIVKTIKSYTLMTIGLFIFVFSWTAFLIPEEIAGGGVSGLA
                     SVIQYSTGIKVFYSYLVINAFLLGIGFLILGNSFGFKTIYCVAVAALFFKFLPFIPWV
                     TDIDDKLINALIGGTLSGIGIGIIFLQGGSTGGTDIIALIISKYKETSPGRVFLFCDL
                     VIIGSVIFIPGKSLSDVIYGYIEMVSFSYVVDM"
     gene            complement(589..1377)
                     /locus_tag="P5293_07175"
     CDS             complement(589..1377)
                     /locus_tag="P5293_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRR49717.1"
                     /translation="MVLQAFQVDAFTDRIFSGNPASVVFLEEKLPDVLLGKIARENML
                     PETAFVLPAGKGTFSLRWFTPDIEIDLCGHATLATSHVIYSWYKHKEEEIIFNTRSGQ
                     IKVRREGDMYAMDFPVREAHKASLPEEIYDSLSIKPNEVYKARDYMLVYSTQEEIELL
                     EISRSIFDEINIDPGGVAVTAPGRDCDFVSRFFTPQATILEDPVTGSAHCTLAPYWSE
                     KLGKERLLAKQLSARGGIIDCTVSEGIVILRGKAVIYSKSEIYL"
     gene            complement(1377..4139)
                     /gene="leuS"
                     /locus_tag="P5293_07180"
     CDS             complement(1377..4139)
                     /gene="leuS"
                     /locus_tag="P5293_07180"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRR49718.1"
                     /translation="MEYNFLEIEKKWQKYWSDNEVFKVEIDPSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGYNVLHPMGYDAFGLPAEQYAIQTGQHPAVTTEK
                     NIARYREQLDRIGFSYDWSREIRTCDPSYYKWTQWAFLEMFAHWYNIETDRAEKISDL
                     ITVFEKEGNLNIKASCSNVKKFTADEWNQFSEKEKADILMCYRIAYLGETSVNWCAAL
                     GTVLANDEVKDGISIRGGYPVEQKKMRQWQLRVSAYAERLLKDLDSIDWTDSLKEMQR
                     NWIGRSEGAEMIFKVSNGLQEFDMEIFTTRADTVFGVTFMVLAPESDWVDKLTTPQQR
                     KAVEDYVNYCKKKTERERIAETRKVTGIFTGSYAINPFTNEKIPVWISEYVLAGYGTG
                     AIMAVPAHDSRDYAFAKQFNLPIVPLIEGCDVSDESFDAKSGIMCNSGFLNGLSVEQA
                     IPAAIEEVEKRGIGWRKVNYRLRDAIFSRQRYWGEPFPIYYKDGIATPLGFEHLPLKL
                     PEIDKFLPTEDGEPPLARAGNWTFEGYPLEKSTMPGFAGSSAYYLRYMDPHNDNSLVS
                     KEADEYWKNVDLYIGGTEHATGHLIYSRFWNKFLFDLGYVCESEPFKKLVNQGMIQGR
                     SNFVYRIKGTNKFVSCGLKDKYDTTEIHVDVNIVSNDRLNIDAFRKWMPEFADAEFIL
                     ENGEYICGWAIEKMSKSMFNVVNPDMVCDKYGADTLRMYEMFLGPLEQSKPWDTNGID
                     GVHRFLKRFWRLFFNKDKFSVSYEKASIKEYKILHKLIKKVEDDIENFSFNTSVSAFM
                     IAVNELYETGCNKREILEPMLILLSPFAPHVAEELWSLLGHGDSISKAIFPEYIQSYT
                     VEDTFEYPVAFNGKMRFKLTLKRDLPKSEIEDLVVKSPETLKYIGEGKISKIIVVPNK
                     IINIVF"
     gene            complement(4183..>4609)
                     /locus_tag="P5293_07185"
     CDS             complement(4183..>4609)
                     /locus_tag="P5293_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRR49719.1"
                     /translation="LVHTAANMDIIVSDTSAKQKESVEKHEKSEGERVYTLYDVDKKP
                     SFLNGGEEQFLNRWIYDYIKYPKAAIENGIQGKVIVEFVIEKDGSVTSVKVVKSAGDL
                     LDAEAVKVISASPKWKPGMKGGEAVRVKMAVPVEFKLRK"
BASE COUNT         1393 a         1022 c          749 g         1445 t
ORIGIN      
        1 catatcgacc acataagaga aagagaccat ttcaatgtaa ccataaataa catctgatag
       61 acttttgcca ggaatgaata ttaccgaacc tataattact aaatcacaaa aaagaaaaac
      121 cctgccagga gaagtttctt tgtattttga tataataagt gcaataatat cagttcctcc
      181 tgtactcccg ccctgaagaa atatgatgcc tatgcctatt ccactcaaag tacctcctat
      241 gagagcatta atcagtttgt catcaatgtc ggttacccag ggaataaaag gcagaaattt
      301 aaagaagaga gcagcaacag caacacaata aattgttttg aatccaaaac tgttacctag
      361 aatgagaaac ccgattccca aaagaaaagc attaataacg agataagaat aaaaaacctt
      421 aatcccggta gaatattgaa taacagaagc caaaccactg acgccaccac cggcaatttc
      481 ttcaggaata agaaatgccg tccagctgaa gacaaatatg aacaatccaa ttgtcatcaa
      541 agtataagac tttattgttt taactattga attgtccatt ccccgatttt aaagatatat
      601 ttcagatttt gaataaatga cagctttacc ccttaatatt acaattccct ctgatactgt
      661 acagtcaatt atccctcctc tggctgaaag ttgtttggcc agaagtctct ctttgccaag
      721 tttttcagac cagtatggcg caagtgtaca gtgagcagat cctgttactg gatcttcaag
      781 tattgtagct tggggagtaa agaaacgtga aacaaagtca cagtcccttc ctggagcagt
      841 aactgcaact cctccggggt ctatattgat ttcatcgaaa attgaacgtg aaatttccaa
      901 aagttctatt tcttcctgag ttgaatagac aagcatatag tctctggcct tataaacttc
      961 attgggttta atacttagac tgtcatagat ttcttcaggc aaggaggcct tatgtgcctc
     1021 tctgacagga aaatccattg catacatatc cccttctctt cttaccttaa tttggcctga
     1081 acgggtatta aagatgatct cttcttcttt atgcttgtac caagaataaa tgacatgcga
     1141 agtagccaaa gttgcatgac cacacagatc aatctctata tcaggggtga accacctgag
     1201 agagaatgtt cctttacctg caggtaatac aaatgcggtt tcaggcaaca tgttctccct
     1261 ggcaattttt ccaagcaata catcaggaag tttttcttcg agaaaaacca cagaggcagg
     1321 atttccggaa aaaattctgt ctgtaaaagc gtcaacttga aaagcttgta ataccattag
     1381 aaaactatat taattatttt atttggaact actattatct tactgatttt accttctcca
     1441 atatatttga gagtctcagg agattttacc actaaatctt caatctcact tttgggaaga
     1501 tctcttttca aagtcagttt aaatctcatc ttaccattaa acgccaccgg atattcaaaa
     1561 gtatcctcaa ctgtgtaaga ttgaatatat tcaggaaata tcgcttttga tatgctgtca
     1621 ccgtgtccaa gcagcgacca cagctcctcc gctacatgag gagcaaaggg tgagagaagt
     1681 attaacatgg gttcaagtat ctcacgtttg ttgcaacctg tttcataaag ctcgttgaca
     1741 gctatcatga aggcactcac acttgtgttg aatgagaaat tctcaatgtc atcttcgact
     1801 ttcttaatga gtttgtgcaa aatcttatac tctttaatag atgccttttc atatgaaact
     1861 gaaaatttgt ccttgttaaa gaaaagtctc caaaatcttt ttaaaaatcg gtgaactcca
     1921 tcaataccat tagtatccca aggttttgac tgctcaagag gtcccaaaaa catttcgtac
     1981 attctgagag tatcagctcc atatttatca cacaccatgt caggatttac aacattgaac
     2041 atagacttgc tcattttttc tatcgcccac ccacaaatat attccccgtt ttcgagaata
     2101 aactcggcat ctgcaaattc gggcatccat ttcctgaacg catctatatt gagcctgtca
     2161 tttgaaacaa tatttacatc aacatgtatt tctgtagtat catatttatc cttcaaccca
     2221 catgatacaa acttgttggt ccctttgatt ctgtaaacaa aattggaacg accttgtatc
     2281 attccttgat taacaagctt tttgaatggt tcactttcac acacataacc caaatcgaag
     2341 agaaatttat tccaaaatcg agaataaatc agatgaccag ttgcatgttc agtgcctcct
     2401 atataaagat caacgttctt ccagtattca tcagcctctt tgctaaccag tgagttgtca
     2461 ttgtgagggt ccatatatct caaataataa gcactgctcc ccgcaaaacc aggcattgta
     2521 cttttttcga gcgggtatcc ttcaaatgtc cagttcccag cgcgtgccag aggtggttct
     2581 ccgtcctcag ttggtagaaa tttatctatt tccggcaatt tgagtggtaa atgctcaaat
     2641 cccaatgggg tagcaattcc atccttataa taaattggga atggctctcc ccaatatctt
     2701 tgacgtgaaa atatcgcatc tctgagacgg tagttaacct ttctccaacc aatacctctt
     2761 ttctcaactt cctcaatggc tgcaggtatt gcctgctcga ctgaaagccc gttaagaaag
     2821 cctgagttac acataatgcc acttttggca tcaaaactct cgtctgaaac atcacacccc
     2881 tctatcaaag ggacgattgg caaattaaac tgtttagcaa atgcgtaatc acggctgtca
     2941 tgagcaggaa cagccataat agctcccgtt ccatatcctg caagaacata ttccgatatc
     3001 caaaccggga ttttttcatt tgtaaaaggg ttaatagcat agctgcctgt aaatatgcca
     3061 gttactttgc gtgtttcagc tattctttct ctctctgtct ttttcttgca gtagttaaca
     3121 taatcttcaa ctgcttttct ttgttgtgga gttgtgagtt tatctaccca gtcactttca
     3181 ggagcaagaa ccatgaaagt cacgccaaat actgtatcag cccttgtagt aaatatctcc
     3241 atgtcaaact cctgtaatcc attgctgact ttaaaaatca tctctgcccc ttctgatcga
     3301 cctatccaat tacgctgcat ctctttaagt gaatctgtcc aatcaatgct gtcaagatct
     3361 ttcaaaagcc tttcagcata ggcggagact cttaactgcc actgacgcat ttttttctgc
     3421 tctactggat aacctcctct tatagaaatg ccatccttga cttcatcatt tgcaagaaca
     3481 gtccctaaag cagcgcacca atttacagat gtttcaccaa gataagctat tctataacac
     3541 atgagaatat ccgctttctc cttttctgaa aattgattcc attcatcagc agtaaatttt
     3601 ttaacattac tgcatgaagc ttttatgttt aaattgcctt ccttctcaaa gacagtaata
     3661 agatccgata tcttttcagc tctatctgtt tcaatattat accaatgtgc aaacatctcg
     3721 agaaatgccc actgtgtcca tttataataa gaaggatcac atgtgcgaat ttcccttgac
     3781 cagtcatagg aaaatcctat acgatcgagc tgttctctat atcttgctat atttttttct
     3841 gtagtaactg ccgggtgctg gcctgtctga attgcatatt gttcagcagg gaggccaaac
     3901 gcatcataac ccatgggatg aaggacatta tagcctttta gccgcttata tctgctgtaa
     3961 atgtcacttg caatatatcc aagcggatgt cctacatgta accctgctcc cgaaggatat
     4021 gggaacatgt ccaatacgta atatttcggc tttgaaggat caatttcaac cttaaacact
     4081 tcattatctg accaatactt ctgccacttt ttctcaatct caagaaaatt gtactccatt
     4141 tttcgcttaa ataatataag ctgcaaaggt agtcaaaaaa agctattttc tcagtttaaa
     4201 ttctacagga acagccatct tgacacgtac tgcttctccg cctttcatgc caggtttcca
     4261 ctttggcgaa gccgaaataa ctttaacagc ttcagcatcc aagaggtctc cagcagattt
     4321 gacaactttt actgaagtaa cagagccatc tttctctatg acgaattcta caatgacttt
     4381 cccttgaatt ccattttcaa ttgcagcttt tggatatttt atgtagtcat atatccatct
     4441 gttaagaaat tgttcctcgc caccgttaag aaatgatggc tttttgtcaa catcataaag
     4501 tgtataaact ctttcacctt cagatttctc gtgtttttca acactctctt tttgctttgc
     4561 ggatgtgtcc gaaacaatta tatccatatt agctgcagta tgtacaagc
//
LOCUS       DAOTQL010000127         4452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_15327_length_4452_cov_5.813964, whole genome shotgun sequence.
ACCESSION   DAOTQL010000127 DAOTQL010000000
VERSION     DAOTQL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15327_length_4452_cov_5.813964"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /gene="lgt"
                     /locus_tag="P5293_07190"
     CDS             complement(<1..541)
                     /gene="lgt"
                     /locus_tag="P5293_07190"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRR49720.1"
                     /translation="MISPLFITWGPSPDIFSIGSVHIRYYSILFVSGFIIGFYLFVKF
                     FKREGISVDILDKLLYALLFAAVVGARLGHCLFYEPDYYLANPIEILKIWNGGLASHG
                     GAIGILLTMWWFVAKYGKKYHFDFLWLMDRLGIATSLAGSLIRLGNLMNSEIYGNPTN
                     LPWGFIFTLRGETVPKHPTQ"
     gene            complement(585..1514)
                     /locus_tag="P5293_07195"
     CDS             complement(585..1514)
                     /locus_tag="P5293_07195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRR49721.1"
                     /translation="MLLESPSEWKDYELIDSGDFQKLERFGRYVLIRPEPKALWKPSL
                     SDREWMNMAHTKFTTGAGFGKSGKEDSGTWHTLKKMDEQWLISYNKSELSFRLRLGLT
                     SFKHVGVFPEQAPNWDYIFNATKQLVDKSDGIKPKVLNLFAYTGAASLTARAAGADVT
                     HLDSVKQVVTWSRANMELSSLDNIRWVIEDALSFVKRESKRGNLYKGIIMDPPAYGHG
                     PSGERWKLDEHLFELLTGVAKILAPERSFLVLNLYSNGYSAVLADTLVRSAFLNGKSP
                     DEFSGNMLEYGELFLRDRFGKNLPLSVFVRLNR"
     gene            1623..2888
                     /locus_tag="P5293_07200"
     CDS             1623..2888
                     /locus_tag="P5293_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRR49722.1"
                     /translation="MQKIDNYNFKGKRAIVRVDFNVPLNEQFQITDDTRIRAAIPTLK
                     KVLDEGGSLIIMSHLGRPKGPTDKYSLKHIIPAIEEKIGTKIKFAPDCMGPEAEELSK
                     NLKPGEVLLLENLRFYAEEEGKPRGLAENASEEEKKAAKAEIKKKQEEFTKRLASYAD
                     CYINDAFGTAHRAHASTALIAKYFPNDKMFGYLMEKEIEAIDKVLKSPVHPVTAILGG
                     AKVSTKIGIIEKLFDVVDNMIIAGGMIYTFVKAQGGKIGNSLCEDDQLDLAKSLIEKA
                     KVKNVKLHYLGEVIAADSFSNEANIRICPCDQIPDGWMGMDAAESTVKEWEKIILSSK
                     TVLWNGPVGVFEMEKFSSGTKRVATILAEATKRGVFTLIGGGDSVAAVTQMGFADKVS
                     YVSTGGGAMLEYLEGIELPGIKAITSSDE"
     gene            2881..>4452
                     /locus_tag="P5293_07205"
     CDS             2881..>4452
                     /locus_tag="P5293_07205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR49723.1"
                     /translation="MNNNNSKFYKLAVLAFLAILLAYSNHFSNSFHFDDDHTIVNNPA
                     IRDINVYKFFTDGKTISTLPTNQSYRPFLTLENAIDLKLSKGGGTEVFHIHIFITFIL
                     VCILIFVFVKDLLYKIDFQGKKENWALLAATMFGLLCANAETVNYIFQRAEIDAALFV
                     LAGMVAFTKGGFWRKYQLYLIFPLIGFFAKEMAFVFAPLLLLYLLIFEENMDLLHFYR
                     SEEIKKLIRSLKTALPAILLTIAYFIFYKIMIPETWTTGGDNVSVYKYMITQPLVICH
                     YIVTYFIPYNLSADTDWTVFNSIADHRAITGIILLIGILYLALKSSKNKNTRLFSFGL
                     LWFLISLIPTSSVLPYSEVLNDHRCFIPYIGLTIAAFSGFKYLLDKYFPNALYNRSSK
                     RIVVLIVIIFLAGNAFGVYQRNKVWHDDLSLWKDVTEKSPLNARGWMNYGVALMKNGE
                     YEQAEMKFLKAASLNPVYAFIYINLGIVNDYMGKTELAESYFKRAIECKNFEHVSWYY
                     YGHFLLSKEKLAEAEN"
BASE COUNT         1384 a          921 c          847 g         1300 t
ORIGIN      
        1 gttgagtcgg atgtttagga acggtctccc ctctgagtgt aaaaataaag ccccaaggaa
       61 gattggtagg attaccataa atctctgagt tcataaggtt gcccagcctt ataagagagc
      121 ctgcaagaga tgttgcaatc ccaagcctgt ccatgagcca gagaaaatca aaatgatatt
      181 tcttgccgta tttggcaaca aaccaccaca ttgttaacaa gattcctatt gcaccaccat
      241 gacttgcgag tccaccattc catattttga gtatctctat cggattcgca agatagtaat
      301 caggttcgta aaaaaggcag tgtcccaatc gggcacctac aacagcggca aacaaaagcg
      361 cataaagaag cttgtccaat atgtctactg aaataccctc acgtttaaag aatttaacaa
      421 aaagatagaa acctatgata aacccagata caaacaaaat actgtaatac ctgatatgta
      481 ctgaacctat agagaaaatg tcaggagaag gtccccatgt tataaagaga ggtgaaatca
      541 tcttatatct ttagtttttc aatagttcat tatgattgcc aagttcatct attaagtctg
      601 acaaaaacac tcaatggtaa gtttttgcca aatctgtctc taagaaacaa ctctccatac
      661 tcaagcatat taccactaaa ctcatccggc gacttcccat tcaaaaaggc acttctgaca
      721 agagtgtcgg caagcacagc agagtagccg ttcgaataca aattaagtac caaaaaactt
      781 ctctcgggag caagtatctt agcaacgccc gtaaggagtt caaagagatg ttcatcaagt
      841 ttccatcgct ccccgctcgg tccatggcca tatgccggag gatccataat tataccctta
      901 tacaggttac cccttttgga ttcacgcttg acaaaactca aggcatcttc aattacccat
      961 cttatattat ctagtgaact gagttccata tttgctcttg accaggtaac tacctgtttc
     1021 accgaatcca ggtgagtaac gtcagcccct gctgctctgg cagtcagaga tgctgctcct
     1081 gtatatgcaa ataaattaag aactttgggc tttatgccat ctgacttgtc aaccagctgt
     1141 tttgtggcat taaatatata atcccaatta ggtgcctgtt ccggaaatac ccctacatgc
     1201 ttgaatgatg taagtcccaa tctaagtcta aatgacaatt ccgatttatt gtatgaaatc
     1261 agccactgtt catccatttt tttgagagtg tgccatgttc cggaatcctc tttcccagat
     1321 ttgccaaatc cggcacctgt tgtaaatttg gtatgagcca tattcatcca ctccctgtca
     1381 gataaagatg gcttccaaag agctttaggt tccggacgga ttaaaacata tctgccaaaa
     1441 cgctcaagtt tctgaaaatc accagaatct ataagttcat aatccttcca ttccgaggga
     1501 gactctaaca acattacaat aaaacttaaa atatgtaatt aatggcgcct aaggtaacac
     1561 tttttctaat attatttcta aattcgctac gatataagag tgataacgaa atactttttc
     1621 aaatgcaaaa gattgataat tacaatttta aaggcaagag agcaatagtc cgcgttgact
     1681 ttaatgttcc tcttaacgaa caattccaaa tcacagacga cacccgtatt cgtgctgcaa
     1741 ttcctacact taaaaaagta cttgacgaag gtggttctct gataataatg tcacatctcg
     1801 gcagaccaaa aggaccaact gacaaatatt cattaaagca tattattcct gcaatagaag
     1861 aaaaaattgg cacaaagatt aagtttgcac ctgactgtat gggcccggaa gcagaagaac
     1921 tttccaaaaa cctgaagcct ggagaggtac tcctcctcga aaatcttcgt ttctatgcag
     1981 aagaagaagg gaagcctcgt ggattggccg aaaatgcttc tgaagaagaa aaaaaagcag
     2041 caaaggcgga aattaagaaa aagcaggaag agttcacaaa gaggcttgca tcatatgcag
     2101 actgttacat aaacgacgct tttggcacag cacacagggc acatgcatcc acagccctta
     2161 tagctaaata ttttcctaat gacaaaatgt ttggttactt aatggagaaa gagattgagg
     2221 ccattgacaa agttctcaaa tctcctgtgc acccggttac agctatcttg ggaggtgcaa
     2281 aggtgtccac caaaatcggc ataatcgaaa aactgtttga tgtagttgac aacatgataa
     2341 tcgctggagg aatgatctat actttcgtca aagcacaagg aggtaaaata ggtaattcac
     2401 tgtgcgaaga tgaccaactt gatcttgcaa aatctcttat cgaaaaagcc aaagttaaaa
     2461 acgtaaagct gcactattta ggagaagtta tagctgcaga cagttttagc aatgaagcca
     2521 acattagaat ctgcccatgt gaccagattc ctgatggctg gatgggtatg gatgcagcag
     2581 aatctacagt taaagagtgg gagaagatta ttctctcgtc caaaaccgtt ctctggaatg
     2641 gaccagtcgg tgtctttgaa atggaaaagt tctcttctgg caccaaaaga gttgctacaa
     2701 ttctcgctga agccacaaaa agaggtgttt tcacactaat aggcggagga gactccgtgg
     2761 cagccgttac ccaaatgggg tttgcagata aggtaagtta tgtttccaca ggcggcggag
     2821 caatgcttga atacctcgaa gggattgagc ttcccggcat caaggcaata acatcttcgg
     2881 atgaataaca ataactccaa gttttacaag ctggctgttt tagcttttct tgcaatactt
     2941 cttgcatatt ccaatcattt ttccaacagt ttccattttg acgacgacca cacaatagtc
     3001 aataatccgg ctatcaggga tataaatgtt tataaattct ttacagatgg caaaactata
     3061 agcactctcc ccacaaatca gtcttatcgt ccttttctta ctctggaaaa tgccatcgat
     3121 ctcaagctgt ctaaaggtgg aggaacagaa gtatttcata ttcacatctt tataacattc
     3181 atccttgtct gtatcttgat ttttgttttt gtcaaagatc ttctttacaa aattgatttt
     3241 cagggcaaga aagaaaactg ggcacttctt gccgccacaa tgtttgggct tctttgtgct
     3301 aatgcagaga ctgttaacta tattttccaa agagcggaga ttgatgctgc actgtttgtt
     3361 ttggcaggta tggtcgcttt taccaaaggc gggttttggc gtaaatacca actttacctc
     3421 atatttcctc ttattggttt ttttgccaag gagatggcat tcgtatttgc gccattgctc
     3481 ctgctctatc ttctaatatt cgaagagaat atggatctgc tccatttcta cagatccgaa
     3541 gagatcaaaa aattaattcg atcattaaag acagctcttc cggctatttt gcttacaata
     3601 gcctatttta tcttttacaa aataatgata cctgagacct ggactacggg tggagacaat
     3661 gtaagcgttt ataaatatat gattactcag ccgctggtta tttgtcatta tattgttaca
     3721 tatttcatcc cttacaatct ctctgccgac acagactgga cagtctttaa ttctattgca
     3781 gaccaccgtg ccattaccgg gattatcctt ttgatcggga ttttgtatct agcgttgaaa
     3841 agttcaaaaa ataaaaatac tcgtcttttt tcatttgggc tactctggtt ccttatctcg
     3901 ctcatcccta cttcttctgt tttgccttac tcagaagtat taaacgatca ccgatgtttt
     3961 attccataca ttggattgac aattgctgct ttttctgggt ttaaatatct cctggacaag
     4021 tatttcccca atgcacttta taacaggagc agcaaaagaa ttgttgtttt gattgttatt
     4081 atatttctgg caggtaatgc gttcggcgta tatcagcgaa acaaggtgtg gcatgatgat
     4141 ctttccctct ggaaagatgt tactgaaaag agccctttaa atgcccgcgg atggatgaat
     4201 tatggtgttg ccctaatgaa aaacggcgaa tatgaacaag ccgagatgaa atttctgaag
     4261 gctgcctcgc taaatcctgt ctatgcattc atttacatca atctaggtat tgtcaacgat
     4321 tatatgggca aaacagaatt ggctgaatca tacttcaaga gagctattga atgcaaaaat
     4381 ttcgaacatg tctcttggta ttattatggt cattttctcc taagtaaaga gaaattggcg
     4441 gaggcagaaa at
//
LOCUS       DAOTQL010000128         4330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_15863_length_4330_cov_4.367953, whole genome shotgun sequence.
ACCESSION   DAOTQL010000128 DAOTQL010000000
VERSION     DAOTQL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15863_length_4330_cov_4.367953"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3186)
                     /locus_tag="P5293_07210"
     CDS             complement(<1..3186)
                     /locus_tag="P5293_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49724.1"
                     /translation="MSLVILIPASIYIALQDPNLQTKLTGKIVENLSKNIKADISIGK
                     VCYLFFNKIIINDLSITYNSNIDTLISCKKLSVSFSARDVFFRNRCNIKKISLSDGQF
                     NLITWADKTTNVDRCFNLYKNISKDTTHIFDSKIKINDLRARNIKFTFRDFSSKAPDR
                     HGECLSFKDLVCDISDLRVKDFKNKDKMFHGNLVNLSLVEQKSGFRIDKLSGNAKIGR
                     RGIFIDDLYLFDSKTELRADYLKFHFNGINSFKNFVDSVKIETSLDNAFISFNTLSKL
                     APTLKGNSLSIYASGKIEGTISSLKTEDLTITSESGLSFISLNARISGLPDIRETMLF
                     LDINDAETMASDISRIVSSINSNQPENLFSKLSPLIKYSFRGRVAGLFSDFVANGNLT
                     SSIGDIYMDMLLRENRSEHGLDIEGKLLANNFNIGLLVQNSNIGKTTFRSTLSGLIRD
                     ERYGGSKFNIDSISVRRIDFKDYSYSNIYSVGSYVDNKFDGRVVCHDPNLNFIFQGIF
                     GRSSKKDSYYDFYADVAYADLAALNLDKRDSVSVISFKTLANFTHKVDGDIIGTISAK
                     SIEYKNRDGEFNIGDIIIQSLTNQDNFNLTIKSGFADITYKGSDFITGFVEKVLKTTF
                     DRHIPSLLAKKEWSPNIDSSKHYRLTINTFNTKAINELLLPGLDIAEKSALLVEIDEN
                     DNLNLSLLSKELGYKDYKIHDLSLKLDSYSNHMLSDIKSSGITISKLMLDSNRIVFSA
                     SHDTLSLRTAFSNESDVKNRVLLSTRTNIRREVSSPKPIFDIYVNPSRMIINNQLWSL
                     SPSRILINNNNIQVEGFKIENDKQFFTAEGRVSNSENDSINLKINCFDISLFDAFMKK
                     PLHFTGLFSGDVIFKEAFKKPQIQMHLNGQAVSVAKRDIGSLTMSSKWNNLEKKLDLI
                     VFNDLNGKRTIDISGTYSPKEKYIDITGNFNNLDPGYASPIFTGIVSDITGGINGKIN
                     CKGPINKLVLTSEDASLKDLSFTVDYTKVRYTINGQTRLHENGLVFVNTFITDQYGSK
                     GTINGGITYTHFRNLAFNTTINFSNIELINLT"
     gene            3306..>4330
                     /gene="tsaD"
                     /locus_tag="P5293_07215"
     CDS             3306..>4330
                     /gene="tsaD"
                     /locus_tag="P5293_07215"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRR49725.1"
                     /translation="MDLTILGIESSCDDTSAAVLRNEMLLSNVMAGQEVHSKYGGVIP
                     ELASRAHQQNIVPVVDRALKLAEVSLNDIDVIAFTRGPGLLGSLLVGTSFAKGLSLAT
                     GKPIVDVNHLQGHLLSPFIKEPDKDKEYPHFPFISLIVSGGHTQIIVVESPLKFKIIG
                     QTIDDAVGEAFDKCAKMLCLGYPGGPIVDKYAKLGNSRAFNFAKPKVKGFDYSFSGVK
                     TSLLYFLRDKMNDDKLFISNNINDICASFQKTLIDILMEKLLLAVKKTGIREVALGGG
                     VSANSALRERVSMEGNDRDFKVFLPEIKFTTDNAAMIAIAGYFLFKSARVADLSVVPV
                     SRVMDYK"
BASE COUNT         1311 a          700 c          818 g         1501 t
ORIGIN      
        1 agtgagattg ataagttcta tattggaaaa attaatagta gtattgaaag caagattacg
       61 gaagtgagta tatgtaattc caccattaat ggtacctttc gatccgtact ggtctgttat
      121 aaatgtgttt acaaatacaa gtccgttttc gtgaagtctt gtttgcccat ttatagtata
      181 tcttactttt gtatagtcaa cagtgaatga aagatctttt agagaggcat cttcacttgt
      241 taatacgagt ttgtttatag gacccttgca attaattttt ccgttgatgc ctccggttat
      301 atcagatact attccggtaa aaataggaga agcataaccc gggtccaggt tattgaaatt
      361 acctgtaatg tcaatatatt tttcttttgg agaatatgtg cctgaaatat caattgttct
      421 ttttccattc aaatcgttaa agactatcaa atctaatttt ttctccagat tgttccattt
      481 gcttgacatg gtcagagatc caatatctct ttttgccact gataccgcct gcccgttaag
      541 atgcatttgt atttgtggtt ttttgaaggc ttccttgaaa ataacatctc ctgaaaatag
      601 tcctgtaaaa tgcaatggtt ttttcatgaa agcatcaaac aatgatatat caaagcagtt
      661 tatttttaaa ttaatagaat cattttcaga attggacaca cgtccctcag ctgtaaagaa
      721 ctgcttgtcg ttttctattt taaatccttc tacttgaata ttattattgt ttattaagat
      781 tcttgaaggg gagagagacc ataactggtt gtttatgatc attctcgaag gattgacata
      841 tatgtcaaaa ataggttttg gagaggatac ttctcttctt atgtttgttc gagtactaag
      901 taaaacgcgg ttttttacat cgctttcatt ggaaaatgct gttcgtaagc ttaaagtatc
      961 atggcttgcg gaaaaaacta tcctattgct gtcgagcatc agttttgaga tagttattcc
     1021 cgatgacttg atatcagaaa gcatatgatt actgtaggaa tcgagtttca aagaaagatc
     1081 atggattttg taatctttat atcccagctc tttagataaa agagagagat tcaggttgtc
     1141 attttcatca atttcaacca aaagtgcact tttttcagct atgtcaagtc cggggagaag
     1201 tagttcatta atggcctttg tattgaatgt gttgattgtc aggcgatagt gtttactgct
     1261 gtcaatattt ggactccatt cctttttagc aagaagagat ggaatatgac ggtcaaaagt
     1321 tgttttcaaa actttttcga caaatccggt aatgaaatct gaacctttat atgtaatatc
     1381 ggcaaaacct gacttaatgg taagattaaa gttgtcttga tttgtcagcg attgaataat
     1441 tatgtctcca atattaaact ctccgtctct atttttgtat tcaattgact tagcacttat
     1501 tgttccaata atatcaccat caactttatg agtaaagttc gccagggttt tgaagctgat
     1561 gacagaaaca gagtctcttt tatcaagatt aagggctgca agatctgcat aagcaacatc
     1621 tgcatagaag tcataatatg aatctttttt tgaggaacgg ccaaatattc cctgaaagat
     1681 aaaattcaaa ttgggatcat ggcatactac tcttccgtca aatttattgt caacatagct
     1741 tccaacggag tatatatttg aataggaata gtctttaaaa tcaattcttc tgacagaaat
     1801 gctgtcaata ttaaatttgc ttccaccata tctttcatct ctaattagtc cactaagtgt
     1861 acttctaaat gtggtcttgc ctatattgct gttttgaaca agaagtccaa tgttaaagtt
     1921 attagccaga agctttccct caatatccaa accatgttct gaacgatttt ctcttagtag
     1981 catatccatg taaatatcac caattgatga ggttaaattg ccatttgcca caaagtctga
     2041 aaatagtcct gcaactctgc ctctaaagct atatttaatg agtggggata gttttgagaa
     2101 taagttctcc ggttgattgc tgttgatcga ggagactatt ctcgatatat cacttgccat
     2161 tgtttcggca tcattgatgt cgagaaaaag catagtttct cttatatcag gaagacctga
     2221 tatccttgca tttagactta taaatgaaag tcctgattct gaggttatgg ttaaatcttc
     2281 tgtttttaga gaagagatag tgccttcaat ttttccgctg gcatagatag agagactgtt
     2341 cccttttaat gtgggtgcga gcttagagag cgtattaaaa cttatgaaag cgttgtcaag
     2401 agatgtctct attttgacac tgtcaacaaa atttttgaaa ctgttaattc cattaaagtg
     2461 aaattttaaa tagtctgctc ttaattcagt cttactgtca aacaaatata aatcgtcaat
     2521 aaatattcct cttcggccta tttttgcatt accacttaac ttgtcaattc tgaaaccact
     2581 cttttgttct actaatgaga gatttaccaa gttgccatgg aacattttat ctttgttttt
     2641 aaaatctttt actctcaaat ctgaaatgtc gcatactaaa tctttgaaag acaaacattc
     2701 tccgtgtctg tcgggagcct tagaactaaa atctcgaaaa gtgaacttaa tattcctggc
     2761 ccggagatca ttaattttta ttttactgtc aaagatatga gtagtatcct tgctaatatt
     2821 tttatagagg ttaaagcatc tgtcgacatt tgtcgttttg tctgcccatg ttatcaggtt
     2881 aaattgtcca tcggaaagtg aaattttttt aatgttgcat ctgtttctga aaaaaacgtc
     2941 ccttgcagaa aaacttacag aaagtttttt gcagcttata agggtatcaa tatttgagtt
     3001 gtatgtgata gaaagatcgt ttattatgat tttgttaaaa aacagatagc ataccttgcc
     3061 tatcgagatg tcagctttaa tgttttttga taaattttca acgatttttc ctgttaattt
     3121 tgtttggaga ttaggatctt gaagggcaat gtaaatactt gctggaatga gtattaccaa
     3181 cgacaaaatg ataactgcta ttttttttaa aatgttgata tatgattcgt ttaaatgaca
     3241 aaattaatca aaatagtgga ggagtttgtt aaaaataaac caattttgcg ggatatatag
     3301 tagttatgga tcttacaata cttggaatag aatcatcgtg tgacgacact tctgcagcag
     3361 tacttagaaa tgaaatgttg ctttcaaacg ttatggcagg gcaggaggtt cacagcaaat
     3421 atggcggagt aattcccgag cttgcttcaa gagcccatca acaaaatatt gtaccagtag
     3481 tcgacagggc tcttaagctt gcagaagttt ccctgaatga cattgatgtg atagctttta
     3541 caagagggcc tggtcttttg ggatctcttc tggtcggaac gtcctttgca aaaggactgt
     3601 ctctggcaac aggtaaacct atagttgatg taaatcatct tcaggggcat ctgctatccc
     3661 cttttattaa ggagcctgac aaggataaag agtaccctca tttccctttt atttcactca
     3721 tagtttccgg aggacatact cagataatag ttgtggagag ccccttaaag tttaaaatta
     3781 taggccaaac tatagatgat gctgtgggtg aggcattcga caaatgtgcc aagatgcttt
     3841 gtttgggata ccctggaggg cctatagtgg ataaatatgc aaaacttggc aattccagag
     3901 catttaattt tgcaaagcct aaggtaaaag gattcgatta tagttttagt ggtgttaaaa
     3961 cgtcgcttct ttactttttg agagataaaa tgaatgatga caagttgttc atatcaaaca
     4021 atattaatga tatttgtgct tcttttcaga aaaccctgat tgatatatta atggagaagt
     4081 tacttcttgc cgtcaagaag acgggaatca gggaagttgc ccttggagga ggcgtatcag
     4141 caaattcggc tctaagggag agagtatcta tggaaggaaa tgacagagat tttaaggttt
     4201 ttttaccgga aattaagttt actactgata atgcggcaat gattgcaatt gccggctatt
     4261 ttttgttcaa atcggcgaga gtagcagatt tgagcgttgt tcccgtttca agggtaatgg
     4321 attataaata
//
LOCUS       DAOTQL010000129         4268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16171_length_4268_cov_5.682412, whole genome shotgun sequence.
ACCESSION   DAOTQL010000129 DAOTQL010000000
VERSION     DAOTQL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16171_length_4268_cov_5.682412"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5293_07220"
     CDS             <1..410
                     /locus_tag="P5293_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49726.1"
                     /translation="LKKLGKIYKDVIRQTKSGLRLTDNMVKFNEYLYKNLIVEATEKL
                     VPQDKNMLKTIIRSPRNTTITLKSFSDDYTPEEREIEIVWKPPQILIFYNKDFYELTN
                     YLVDFCDNNGLEFKSDFERQTNTQYFYIWKEKK"
     gene            415..708
                     /locus_tag="P5293_07225"
     CDS             415..708
                     /locus_tag="P5293_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49727.1"
                     /translation="MMFKKFLEGNFFQGEWVSAKNVQGVFIAKISQNESVIAGYNNKE
                     KKYLKINNSTIKKCEGKDADLLSFAKKHILWHHYEKFDRKGSKDKEIRRKAEK"
     gene            complement(686..2128)
                     /locus_tag="P5293_07230"
     CDS             complement(686..2128)
                     /locus_tag="P5293_07230"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRR49728.1"
                     /translation="MVTICKHDFLNIRIETEDYRYLREVRDFLSAYIEGYQWMPAFTH
                     SGWNGKVSMLNLSNKTIPYGLLIDFIKFHKKFHKEIELKIEPDVLNLFRGAEVSPKYN
                     LSLLPHYYQEDCINAALKYKRGIIRSATASGKSLVISYIIKTLFENKLIKKVLIIVPT
                     ISLVEQFYDDLLKYGYFTKTDLGKVYEKYKEFQSKVVISTWQTLSRNHKLLQSFDCLI
                     CDETHGAKAHEIKKILSKCTKADYRLGFTGTLPTPKIDMWNIKAYLGPILREYGAGQL
                     GDEGYISKCNVKIVCIHYKRSFKGTYDEIKDAVFKTPFRLDVIREIIKFVDKNILILV
                     HRVEKEGMMLKQFLEENPIDNREIVFLYGKSKVEEREFWRKECEKRNDIILIATYGIF
                     QLGINIPSLKYIILASPFKSKIRVLQSIGRSLRKHLDKLDGAFVFDIFDNCKYLRDHG
                     IKRERYYNAEGFKIEDIILEEGSTISQPFV"
     gene            complement(2133..2585)
                     /locus_tag="P5293_07235"
     CDS             complement(2133..2585)
                     /locus_tag="P5293_07235"
                     /inference="COORDINATES: protein motif:HMM:NF022508.2"
                     /note="UvsY was described originally in phage T4; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator protein UvsY"
                     /protein_id="HRR49729.1"
                     /translation="MNNNDKDLSENEVEERDKIWKELYEKYPIDKQVQFSELDIQEKL
                     RNQPFLLLHYSDLYYKEKAKYDKMIEVLDKIQGTRYDYYKFNSDKELTKYEIEKFYLP
                     KDPTLLKAKEKVRKQQWRVDFFKMCYDAINNQGWQLKSFIEGVKQGLL"
     gene            2706..3077
                     /locus_tag="P5293_07240"
     CDS             2706..3077
                     /locus_tag="P5293_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49730.1"
                     /translation="MGNINIKAMAVVKNLDGSWNLGVLRKNKFNFIKKISKVEDVPNV
                     KNYLTLKQYLKLQGREISLSKEILEELGVEKMFLLPKSYEKIYPISQEKNKSLCNLCK
                     HSCQMAKDQIIYKCSQFEQVN"
     gene            3077..4120
                     /locus_tag="P5293_07245"
     CDS             3077..4120
                     /locus_tag="P5293_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49731.1"
                     /translation="MEPRFLEKIILKGCLLDKGFLATITSTFRAEYFDDPVISEIFSF
                     LKNHLEQYDKIPPRDAIRATLSHHDDEIREIFTEMDTIDFDVAKNFEYLVKESNEYLK
                     DKAVKRAILDSVDIINSGKIEERGKIRELVELALAKDLRIDIGLDYFDMLGERLKRIF
                     TTSSNRIQTYFPQFDEYLNGGFPPFTFSVLVARVHGFKCVGGNTEVLIKDRMNNTFPI
                     RIQDFYKYIETMNKYKNGEIDKMPSINSFIRKYGDEEGKKKYLSWKENVSIASKNRNT
                     LEFFISKYGEKVGNERYNLYIEKQKISHSEQIRGRKKHKEISSRSGNNKNSYIKKYGE
                     EIGIRKWEERQKR"
BASE COUNT         1428 a          639 c          765 g         1436 t
ORIGIN      
        1 cgcttaaaaa acttggtaag atatataaag atgttattag acaaactaaa tcgggattaa
       61 ggttgacgga taacatggta aaatttaatg aatatttgta taaaaatttg atcgtggagg
      121 ctacagaaaa attggttccc caagataaga atatgttgaa aaccatcatt aggagcccgc
      181 gcaataccac cattacactc aaatcatttt cagacgatta tacaccagag gagagggaaa
      241 ttgaaattgt ctggaaacca ccacagattc ttatattcta caataaagac ttttatgagc
      301 taaccaatta tcttgttgac ttttgcgaca ataatggcct tgagtttaaa tctgactttg
      361 agcgccaaac taatacacag tatttttata tttggaaaga gaagaaataa acttatgatg
      421 tttaaaaaat ttttagaagg aaattttttc cagggtgaat gggtctcggc taaaaatgtt
      481 cagggagtgt ttattgcaaa gatatctcaa aatgaatctg taattgcagg atataataat
      541 aaagaaaaaa agtatctgaa gataaataat agcacgatta aaaaatgtga aggtaaggat
      601 gctgatcttc tttcatttgc caagaaacat attctctggc atcattacga aaaatttgat
      661 aggaaaggaa gtaaggataa agagattaga cgaaaggctg agaaatagtt gaaccctcct
      721 caagaataat atcctcgatt ttaaatccct ctgcattata atatctttcc ctttttatac
      781 catgatccct caagtatttg cagttatcga aaatatcaaa tacgaaagcc ccgtcaagtt
      841 tgtctaaatg tttcctcaaa cttctaccta tagattgtag aactcttatc ttactcttga
      901 atgggcttgc aaggatgata tattttaacg atggaatatt gataccgagt tggaatattc
      961 cataagttgc aatcaagata atatcgtttc ttttttcaca ttcctttctc caaaattctc
     1021 tttcttcaac ttttgatttt ccatatagaa acactatttc ccgattgtct ataggatttt
     1081 cttcaaggaa ttgttttaac atcattccct ctttttctac acggtggaca agtattagaa
     1141 tgtttttgtc cacgaatttg ataatttctc tgataacatc caacctgaat ggagttttaa
     1201 aaacggcgtc ttttatttca tcataggttc ccttgaagga tcttttatag tgtatacata
     1261 ctatcttcac attgcacttg ctgatatatc cttcatctcc taattgcccc gctccatatt
     1321 ctctcaatat aggcccaaga tatgctttaa tattccacat atcaattttt ggagtgggta
     1381 atgttcccgt gaagcccagg cggtagtccg ccttcgtaca tttactgagt attttcttga
     1441 tttcgtgtgc cttagctccg tgtgtttcat cacatatcaa gcaatcgaag ctttgaagaa
     1501 gtttgtggtt tctacttaat gtctgccaag tagatatgac aacttttgat tgaaactctt
     1561 tatatttttc ataaactttt cccaaatcgg ttttggtgaa atatccatat ttcagaagat
     1621 catcatagaa ctgctctacc aaagaaattg taggaacaat gatgagaact ttttttataa
     1681 gtttattttc gaagagtgtt ttaatgatgt aagatatgac cagcgactta ccacttgctg
     1741 ttgcagaccg gatgatacct cttttatatt tcagggctgc attgatgcaa tcttcctgat
     1801 aataatgtgg taaaagggac aaattatatt ttggggaaac ttctgctcca cggaaaaggt
     1861 tcaatacatc aggttctatt ttcagttcta tttccttatg aaattttttg tggaatttta
     1921 tgaagtctat gagtagacca taaggaatgg ttttattgga aagattcaac atggagacct
     1981 taccattcca accgctatgg gtgaatgcgg gcatccactg gtaaccttct atatacgccg
     2041 aaagaaagtc tctgacctct ctcaaatacc tgtaatcttc tgtttctatt ctgatattca
     2101 gaaagtcatg tttacaaatt gttaccattg ggttataata gaccttgttt gacaccttcg
     2161 ataaaacttt ttaattgcca tccctgattg tttatggcat cgtagcacat tttgaaaaag
     2221 tcaactctcc attgttgttt tctaactttt tctttagcct tcagaagagt tgggtcttta
     2281 gggagataga atttttctat ttcgtacttg gttaattctt tatcactgtt gaatttatag
     2341 taatcgtatc tggttccctg tattttgtcc agaacctcta tcattttgtc gtatttggct
     2401 ttttctttgt aatagagatc actatagtgg agtaaaagaa atggttgatt tcgaagtttt
     2461 tcctgtatat ccagttcaga gaactgtact tgtttgtcta ttgggtattt ttcgtacagt
     2521 tctttccaga ttttatctct ttcctcgact tcattttctg atagatcttt gtcattatta
     2581 ttcattgaaa accctcatgt ataggtattt atattattat aacatacgat attgcaagcg
     2641 taaacttcta tttacattat tgtggatttg tgttataata taacagtaaa attgtggagg
     2701 caaatatggg aaatataaac ataaaagcca tggcggttgt gaagaattta gacggtagtt
     2761 ggaaccttgg agttttgaga aaaaataagt ttaattttat aaagaaaatt tccaaggtcg
     2821 aggatgtacc aaatgtcaaa aactatttaa ccttgaagca gtatcttaaa ctacagggga
     2881 gagaaatttc tttatccaaa gaaattttgg aagaattggg tgtggaaaag atgtttcttc
     2941 tgccaaaatc ttatgaaaag atttatccga tttctcaaga aaaaaataag agtctttgta
     3001 atttatgtaa acactcttgt caaatggcta aagatcagat aatatataag tgcagtcagt
     3061 ttgaacaggt gaattgatgg aaccaagatt tcttgaaaaa attatcttga agggatgttt
     3121 acttgataag gggtttcttg cgacaattac atctacattc agagcggaat attttgatga
     3181 tcctgttatt tcagaaatct tctcttttct aaagaatcat cttgagcagt atgataaaat
     3241 tccgccgagg gatgctatta gggccactct gtcacatcat gatgatgaaa tcagagaaat
     3301 atttacagaa atggatacta ttgattttga tgtggcaaaa aacttcgaat atcttgtcaa
     3361 agaatctaat gagtatttga aggataaggc cgttaagaga gcaatcctcg attcagttga
     3421 tataatcaat agtggaaaaa tcgaagagag gggaaagata agggaacttg ttgagctggc
     3481 tctcgcaaag gatttgagga ttgatatcgg acttgattat tttgatatgc ttggtgagag
     3541 gctgaaaaga attttcacta catcatcaaa caggattcaa acatattttc ctcaatttga
     3601 tgaatatctg aatggtggat ttccaccatt tactttttct gttttggttg cccgtgttca
     3661 cgggttcaaa tgtgttggtg gaaacaccga agttcttata aaagacagaa tgaataatac
     3721 ctttcctata aggatacaag acttttataa atatattgaa accatgaaca agtataaaaa
     3781 tggagaaatt gataagatgc cgtctataaa ttcttttata agaaaatatg gtgatgaaga
     3841 aggcaagaaa aaatatcttt cttggaaaga aaacgtaagt attgcaagta agaatagaaa
     3901 tacattagag ttttttatat cgaaatatgg tgagaaagtt ggcaatgaaa gatataattt
     3961 atatattgaa aaacagaaaa tttcacattc tgaacaaata agaggtcgta aaaaacataa
     4021 agagatttca tccagaagtg gaaataataa aaattcttat attaaaaaat atggagaaga
     4081 gattggcata agaaaatggg aagaaagaca gaaaaggtga ttgcaaacaa tatttaaaaa
     4141 aaccgcggcg gataaatgga aagaagatga ggaacgaata gatataattc ataaacaagg
     4201 atataatgtt tacaaagaat ttgcatacat ggtcttttta tcgtcttgct aaatatatag
     4261 aatataaa
//
LOCUS       DAOTQL010000130         4265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16182_length_4265_cov_4.615677, whole genome shotgun sequence.
ACCESSION   DAOTQL010000130 DAOTQL010000000
VERSION     DAOTQL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16182_length_4265_cov_4.615677"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="P5293_07250"
     CDS             <1..331
                     /locus_tag="P5293_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRR49732.1"
                     /translation="SGRIRINEIQAKPSKEIKPGDIISVRRGNIHFTYRVIADVERRQ
                     PAKMVDQYIDNITPQEELKKLNLPKESFFFSRKRGTGRPTKKERRDIDELFDNLDFYY
                     DNETEGD"
     gene            complement(328..1317)
                     /locus_tag="P5293_07255"
     CDS             complement(328..1317)
                     /locus_tag="P5293_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRR49733.1"
                     /translation="MATLYPLKFKPIVKERVWGGDQLIKLLGKPYSGNNPVGESWELS
                     GLHGDTSVIKNGFLKKNDINEIVETYLGDITGDAIYDRFGMEFPILIKYLDIQKELSI
                     QVHPDDETALVRHDSNGKTELWYIVDANPDAKIYMGFNRDTTPQELFDKCNNDTVNEI
                     LNVITPKKGDYVLIKPGTIHSATGGILVAEIQTTSDITYRVYDWGREHHPETAREMHL
                     DLAIDIIDYKKYDLSQYHSADYKNIEHEILVDCNYFKISKLNPNKTLYYESTQFESFI
                     IYMLIEGEITINSKNSLLDVSKGETVLIPAGLGEYSILKKSAECTLLEITGKS"
     gene            complement(1277..2113)
                     /locus_tag="P5293_07260"
     CDS             complement(1277..2113)
                     /locus_tag="P5293_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRR49734.1"
                     /translation="MLKKFWPYIPDLKESWILVIILLIGGTALSTILTIIMRFAFPSL
                     TEWYGTIAYPFIFLLPYLWIVRSIKKQQSNILLPLNSPNFGKMGRIIFFILLIPLLLS
                     FNYVTEPLSSWMTVPDFIKRLFESVTENKLSSLIMMVILAPLFEETFCRGIILRGLLK
                     HMNPLKAIFWSALIFGVIHLNPWQAIPAFLVGSLMGWIYWKSGSLWATIFMHFINNGF
                     SYLIVILFPELPADAGFKDLFPGNMYYIYYLLSLIILISILILINRNYGNTLSTKIQT
                     NS"
     gene            complement(2136..3047)
                     /locus_tag="P5293_07265"
     CDS             complement(2136..3047)
                     /locus_tag="P5293_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRR49735.1"
                     /translation="MVKPTLLVLAAGMGSRYGGLKQLDSVGPNGETIMDYSVFDAVRA
                     GFGKIVFTIRHGFRKQFEDAVKQRYGNNVEYDFVEQELENIPDRLKYNPLRSKPWGTG
                     HAVLVAKDVIDTPFAVINADDFYGCNSFQVLGHFLEECEGNTGTFAMVGFNIANTLSE
                     SGSVSRGICSVNSEGYLTDVEEHHNIARKDKSIYGLNPRNELVEIVPETLVSMNMWGF
                     TLDLFTLGEELFTEFLLQNGEDLSAEFYIPYVVNRIIKSNKASCKVLSTPDQWFGVTY
                     KEDRENVVTRLSKLIERKIYPSPLFKK"
     gene            3117..3425
                     /locus_tag="P5293_07270"
     CDS             3117..3425
                     /locus_tag="P5293_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRR49736.1"
                     /translation="MWQRVKESGFYKLIKNKFFIATLIFAVWVIFIDKNSLIDWFHIR
                     LNIVRQERQIEYYKNEIKSIDEKLQELSSNLDSLEKFAREQYYFHEDDEDLFIVEEKK
                     "
     gene            complement(3403..4116)
                     /locus_tag="P5293_07275"
     CDS             complement(3403..4116)
                     /locus_tag="P5293_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRR49737.1"
                     /translation="MKSQKKYRYFLFDLDRTLWNFDLNAKRNIFRLIDKYSLPISSKE
                     SFYNDYEMINHFLWDQYEKGIITKELLRSERFRQTLLKYNIDSPLLAKIIGEEYLDTM
                     PNQTALMPYAAEVLAELKRAGCKIAIISNGFKEVQYKKLKNSGIEQFFDAVLISEEQG
                     IHKPSPIIFKRALRALNGIKEEAIMTGDDFANDIEGAMIFGIDQFYYNYKNLPCEGGP
                     TYNSDDLRDLLKLTFSLPQ"
     gene            complement(4119..>4265)
                     /locus_tag="P5293_07280"
     CDS             complement(4119..>4265)
                     /locus_tag="P5293_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HRR49738.1"
                     /translation="VGLYEGGGYMAKGIFRPAQDCRMKTNTSPSFCPVCVRAINRMID
                     YYTK"
BASE COUNT         1364 a          787 c          735 g         1379 t
ORIGIN      
        1 atcaggtcgt ataagaataa atgaaattca ggccaaacct tctaaagaga ttaaacctgg
       61 agacataata tctgtccgaa gagggaacat tcattttacc tacagagtta ttgcagatgt
      121 cgaacgcaga cagcctgcta aaatggttga tcagtatatc gacaacataa ctccacaaga
      181 ggagttgaag aaattgaacc tgcccaaaga gtcgtttttc ttttcaagaa aaagaggaac
      241 agggagacct accaaaaaag aaagaagaga tattgacgag ctttttgaca atcttgattt
      301 ttactatgat aacgaaacag aaggagatta acttttccct gttatttcga gtagcgtaca
      361 ctctgccgat ttttttaata ttgaatactc tccaagtcca gcagggataa gcacagtttc
      421 tcctttagaa acatcaagta aggagttttt ggagttaatt gttatttctc cttctattag
      481 catgtatatt ataaaacttt caaactgtgt gctttcgtag taaagagtct tatttggatt
      541 gagttttgag atcttaaaat agttgcagtc aacaagtatt tcatgctcta tatttttgta
      601 gtcagcgcta tgatattgag agagatcata ttttttataa tctattatat ctattgccag
      661 atcaagatgc atttctctgg cggtttcagg gtgatgttct ctgccccagt cataaacacg
      721 gtaggtaata tcagaagttg tttgaatttc agccactaaa atgcctcctg tagcagaatg
      781 aattgttccg ggtttaatta gaacataatc tcctttttta ggagtaataa cattgagtat
      841 ttcattgaca gtgtcattgt tgcatttgtc aaacaattcc tgaggggtag tatctctgtt
      901 gaaacccata tatatttttg catcgggatt agcatcgaca atataccata actctgtttt
      961 accatttgaa tcatgcctta caagagctgt ttcatcatca ggatgtacct gtatcgacaa
     1021 ttctttctga atatccaaat atttgatcag tattggaaat tccattccga atctgtcata
     1081 aattgcatca cctgttatat ctccgagata ggtttctaca atctcgttga tatcgttttt
     1141 tttaagaaat ccatttttaa ttactgatgt atcaccatgt agaccactta attcccagct
     1201 ttctccgaca ggattgtttc ctgaataagg tttccctaat aattttatta actgatcgcc
     1261 accccaaact cgttctttaa ctattggttt gaattttagt ggatagagtg ttgccataat
     1321 tgcgatttat taaaatgaga attgagatca agataataag agaaagtaag taatatatat
     1381 aatacatgtt tccagggaac aggtctttga agcctgcatc agctggtagc tcaggaaaca
     1441 gaataacaat aagatatgaa aacccattat taataaaatg cataaaaatt gtagcccaca
     1501 gagaaccgct tttccaatag atccatccca taagtgaacc taccagaaag gccggtatag
     1561 cctgccaagg attaaggtgt attacaccga atattaatgc agaccaaaaa atagctttta
     1621 atggattcat atgtttcaga agtcctctaa gaataatccc cctgcaaaat gtctcttcaa
     1681 acaatggtgc aagtattacc atcattatca agctgctgag cttgttttca gtaacacttt
     1741 caaaaagtct cttgataaaa tctggaacag tcatccacga tgagagtggt tcggtaacgt
     1801 aattaaacga taaaagcaat ggtataagta gaataaaaaa tattattctt cccattttgc
     1861 cgaaatttgg actgtttaaa ggaagtaaaa tgttggattg ttgcttttta atagatctta
     1921 caatccacaa ataaggcagt aaaaatataa atggataagc aatagtccca taccattctg
     1981 taagtgaagg aaaagcaaat cgcataataa ttgtgagtat tgtgctcagg gcagtacccc
     2041 caattaatag aattataaca agtatccagc tctcttttaa atctggaata tacggccaga
     2101 attttttaag cattaattaa atattagttg catttttact ttttaaaaag gggagatggg
     2161 taaatcttcc tttcaatcag ctttgaaagt ctggtaacaa cattttcccg atcttccttg
     2221 taggtaactc caaaccattg gtcaggagta ctcaatactt tacaggaggc tttattgctt
     2281 ttgataattc tgtttacaac ataaggaatg taaaattcag cagataaatc ttctccattt
     2341 tgtaatagaa actccgtgaa aagttcttct cccaatgtaa ataaatccag cgtaaatccc
     2401 cacatattca ttgataccag agtttcaggt actatttcta caagttcatt tcgcggatta
     2461 aggccataaa ttgatttatc ctttcttgca atattatgat gctcttctac atcagtaaga
     2521 taaccctccg aattgactga acatattccg cgtgacacac tgccggattc agaaagagtg
     2581 ttggcaatat taaagcctac catggcaaaa gtccctgtgt ttccttcaca ctcttcaaga
     2641 aagtgaccaa gcacctggaa cgagttacag ccataaaaat cgtcagcgtt aatgacggca
     2701 aaaggagtgt cgataacatc ttttgcaacc aatacggcat gtccagtccc ccaaggctta
     2761 cttctaagcg ggttatattt gagcctgtca ggaatatttt ccagttcctg ctctacaaaa
     2821 tcatattcaa cattattccc gtatctttgt tttacggcat cttcaaattg ttttctgaaa
     2881 ccatgtcgta tggtgaagac tatctttccg aaaccggctc tgacagcgtc gaaaacagag
     2941 taatccataa tcgtttcgcc attaggccca acactgtcaa gttgttttaa tcctccatat
     3001 ctgctgccca tgccggcagc aagaacaagt aatgttggtt taaccattca ttattatatt
     3061 aattatctta gacaaaatta ttactatttt tgcgaaaaca actatttaaa acaaagatgt
     3121 ggcaaagagt taaggaatcc ggattttata agcttattaa aaacaagttt tttatagcca
     3181 ctcttatttt tgctgtttgg gtaatattca tcgataagaa cagcctgatt gactggtttc
     3241 acataagact gaatattgtt agacaggaac gacaaatcga atactacaaa aacgaaatca
     3301 aatcaattga cgaaaaactt caggagctga gttcaaatct tgacagtttg gagaaatttg
     3361 caagagaaca gtactatttt catgaagatg atgaagatct ttttattgtg gaagagaaaa
     3421 agtaagtttc aacaagtctc taagatcatc gctattgtag gtagggcccc cttcacaagg
     3481 aagattctta taattataat agaactggtc aatgccaaat atcattgctc cttcgatatc
     3541 attggcaaaa tcgtctccgg tcataatagc ttcttcttta atcccgttca aagctcttag
     3601 tgctcttttg aatattatag gagagggctt atgtattccc tgctcctcag aaatcaggac
     3661 agcatcgaag aactgttcaa tcccggaatt tttaagtttt ttatactgta cttctttaaa
     3721 gccattagag ataatcgcaa ttttacaacc ggctcttttc aactccgcaa ggacttctgc
     3781 tgcgtaaggc attaaagcgg tttggtttgg cattgtatcg agatactctt ctccaattat
     3841 ttttgcaagc aaaggactgt caatattgta cttaagcagc gtctggcgaa atctttcact
     3901 tcttaacaat tcttttgtaa ttatgccttt ttcatattga tcccataaaa aatggtttat
     3961 catttcgtaa tcattataaa aagactcttt gctcgaaatc ggtagtgagt atttgtcaat
     4021 cagcctgaat atattccttt tggcgttaag atcaaaattc cataaggtac gatcgaggtc
     4081 gaatagaaaa tatcgatatt ttttttgaga tttcacctct atttagtata atagtcaatc
     4141 attctgttaa ttgctcttac acataccgga cagaaagatg gtgaagtgtt ggttttcatt
     4201 ctacagtctt gagcaggcct gaatattcct ttggccatgt atcccccacc ttcataaaga
     4261 ccaac
//
LOCUS       DAOTQL010000131         4215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16421_length_4215_cov_4.853125, whole genome shotgun sequence.
ACCESSION   DAOTQL010000131 DAOTQL010000000
VERSION     DAOTQL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16421_length_4215_cov_4.853125"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="P5293_07285"
     CDS             complement(<1..1108)
                     /locus_tag="P5293_07285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRR49739.1"
                     /translation="MNNFVFQNPTKLIFGKGTIEHLSTEIPANKKVFVTFGGGSVKTN
                     GVYQQVKRALTGKDHIEFWGIEPNPTVETLRKAIAIVKEENCDFILAVGGGSVIDGSK
                     LIAAAAMIEEDAWELVKRPSLYKGSLPLASVLTIPATGSEMNPGAVITNSALKEKFSF
                     YSTFPVFSILDPQVAFSLPKYQIACGLTDTFVHVLEQYLTTTNQSPLMDRWSEGILQT
                     IIEVAPKVYGDKPTYDDMATFMLSATMALNGFISMGVSQDWSTHMIGHELSALHNLTH
                     GHTLAIVLKGTMWVMKEEKGDKIVQMGKRVWNIEESDREKIIEKTIDKCDAFFRSLGL
                     KTRLSENGIGKETIDEIVRRFEKRNVHFGENKTID"
     gene            complement(1193..1265)
                     /locus_tag="P5293_07290"
     tRNA            complement(1193..1265)
                     /locus_tag="P5293_07290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1230..1232),aa:Lys,seq:ctt)
     gene            1333..3000
                     /locus_tag="P5293_07295"
     CDS             1333..3000
                     /locus_tag="P5293_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HRR49740.1"
                     /translation="MEWFHNLFLGANVAQSVIILSIVITLGLILSKIKVAGISFGVTW
                     ILFAGIAFSHFGLNINTETLHFVKEFGLILFVYSIGLQVGPGFFASLKRGGLKLNMLA
                     VLIVILGVVVTLVIKYISGLPVSTITGIMSGAITNTPGLGAAQQTYTEIYGSSDPSIA
                     TGYALAYPLGVVGIILSMIAIKRIFSITPKSENERIMEQQKNNPIETVRCSIVIENSR
                     LVGKTIEEIKPLLDADYVISRVKGRDDKIVIAKADTLINEGDRILVIAQKRDTEKIIN
                     FFGREIQMTEEEWGIFNVDIVPRRVLVTNNKVNGKTLAELKLGKAFGITVTRINRAGV
                     DLLAKPNTALQIGDRLTIVGTQESIDGAAKLLGNSMKKLREPNLIAIFLGIVLGIFLG
                     SIPIMIPGVPQPIKLGLAGGPLIVAILIGKFGPKFKIVTYTTVSANLMLREVGISLFL
                     ACVGLDAGENFVETIVNGGGAMWILYGFFITVIPILIVGIIGRVVYKLDYFTLMGLIA
                     GSTTDPPALAYANSASDNDLPAVSYATVYPLTMFLRVLAAQILVLFC"
     gene            3028..3102
                     /locus_tag="P5293_07300"
     tRNA            3028..3102
                     /locus_tag="P5293_07300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3061..3063,aa:Arg,seq:cct)
     gene            complement(3111..4058)
                     /locus_tag="P5293_07305"
     CDS             complement(3111..4058)
                     /locus_tag="P5293_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRR49741.1"
                     /translation="MEEDLLLRFIEGKASPKEQREIVEWIEQNEENKKIFAQLKNLSV
                     ASEILSEEQRERDLIQRQRLRMIYSWSLKIASILIIAFSCFYFGKKHEYTNWIETSEL
                     KNSVISVPLGESVCLTLPDGSTVSLNSGSTLKYTGLYGINEREVYLDGEGYFKIKKGE
                     KKFIVKTDIINVEVVGTEFNLSAYNEDRQISTSLFSGKVEIKNLTKSEDIILEPNYCY
                     TFDKITKKTIVKNITRRQTWTENYFVAESDDIISFVKKIERKYKVKIIVSPELSEKCI
                     YTGTFKGESLIDILNNMSLASPISYEIKNDSTVIINPRK"
BASE COUNT         1242 a          741 c          755 g         1477 t
ORIGIN      
        1 agtcaattgt tttattttca ccaaagtgaa cgtttctctt ctcaaatctt cttacaatct
       61 cgtcaatggt ctccttacca atcccgtttt cggataatct tgttttaagt cctaaagaac
      121 ggaaaaatgc atcacattta tcaattgtct tttcaattat tttttctctg tctgactctt
      181 ctatgttcca aactctttta cccatttgaa ctattttatc acctttttct tctttcatta
      241 cccacattgt gcctttaagc actatagcca aagtatggcc atgtgtcaga ttatgaagtg
      301 cacttaattc atgccctatc atatgagtag accaatcctg agatacgccc attgaaatga
      361 atccattcag agccatagtt gcagaaagca tgaaagttgc catgtcatcg taagtgggtt
      421 tgtctccgta aactttggga gccacctcga taattgtctg aagaatccct tcagaccatc
      481 tgtccataag cggggattga tttgtggtag taagatattg ctccaaaaca tgtacaaaag
      541 tatcagtcag tccacaggct atttgatatt ttggaagtga aaatgcaacc tggggatcga
      601 gaattgaaaa aacagggaaa gtactgtaaa atgagaattt ttctttaaga gcactgttgg
      661 taataacggc accaggattc atttcagatc cggttgcagg tattgtcaat acgcttgcca
      721 gtggaagtga tcccttgtat aatgaaggtc ttttgactag ttcccatgca tcctcttcga
      781 tcatggctgc tgctgcgatt agttttgatc catcaattac agatcctcca cctacagcaa
      841 gaataaagtc gcaattctct tctttaacta tggcaatggc ttttctaagt gtttcaactg
      901 taggattagg ctcaattccc cagaattcta tgtgatcttt tccagttaat gctcttttaa
      961 cctgttgata tacaccgttg gttttaacac tgcctccgcc aaaagtcaca aaaacttttt
     1021 tattggcagg tatttcagta ctaagatgtt caatagtccc tttcccaaaa atcagtttag
     1081 tgggattttg aaaaacaaaa ttattcattt ttaaaaattt ttattactta tttattgttg
     1141 taaaattaaa taaaaaactg caatcaacat ttgattacag tttacatttt tgtggtcccg
     1201 gtgggtctcg aacccacgac cccaacatta agagtgtcgt gctctaccaa ctgagctaca
     1261 gaaccatttt ttaggtttgc aaagatacaa atattttaaa attaattggc taattttgta
     1321 aaaaatatag taatggagtg gtttcacaat ctttttttgg gagctaacgt ggctcaatct
     1381 gtaattattc tttcaattgt tataactctt ggactgattt tgtcaaaaat caaagttgcc
     1441 ggcatctctt ttggtgttac ctggattttg tttgccggaa ttgctttcag tcattttggt
     1501 ttgaatatta ataccgaaac acttcatttt gttaaagaat ttggattaat tctatttgtt
     1561 tatagcattg gcttacaggt agggccaggt ttttttgcct cactcaaaag agggggtttg
     1621 aaacttaata tgcttgcagt actgattgtt atactgggtg tagttgtaac gttagttatt
     1681 aaatatattt caggattacc tgtctctaca ataaccggta tcatgtctgg tgctataaca
     1741 aatacacctg ggctcggtgc tgcccaacag acgtatactg aaatttacgg ttcatcggat
     1801 ccctctatag ccacaggata tgctcttgct tacccgttgg gtgtagtagg cataattctt
     1861 tcaatgatag caataaaaag aatcttttcc attacaccta aatcggaaaa tgagcggatt
     1921 atggagcagc aaaaaaataa cccgattgag acagtcaggt gttctattgt tatagaaaac
     1981 agcagattag ttggtaaaac aatagaggaa ataaaacctc tccttgatgc cgattatgta
     2041 atatcgagag taaaaggaag ggatgacaaa attgttattg ccaaggcgga tacacttatt
     2101 aatgagggag acagaatttt ggtaattgct cagaaaagag acacagaaaa aattattaat
     2161 ttttttggta gggagattca aatgaccgag gaagaatggg gtatctttaa cgttgatatt
     2221 gtgccaagga gagttttagt cacaaacaat aaagtaaacg gaaaaactct tgcagagcta
     2281 aaattgggaa aggcttttgg aattactgtg acaaggataa acagagcagg cgtggatctt
     2341 cttgcaaaac ccaatacagc tttacagata ggtgacagac ttacaattgt aggtactcaa
     2401 gagtcaattg atggtgccgc taaacttctt ggcaattcca tgaagaagct tcgtgagcct
     2461 aatcttattg caatatttct gggtattgtt ctcggaatat ttttgggttc gattccaatc
     2521 atgattccag gcgtaccaca acccattaaa ctaggtcttg ccggaggtcc actgatagtt
     2581 gcaattttaa ttggcaagtt tggacctaag ttcaaaatag ttacatacac cactgtgagt
     2641 gccaatttaa tgttgagaga ggtgggtatt tctctttttt tggcatgtgt cggacttgat
     2701 gcaggtgaaa attttgttga aacaattgta aatggcggtg gtgctatgtg gattttatat
     2761 ggtttcttta ttacagtcat accaattttg attgttggaa tcattggcag ggttgtttat
     2821 aaacttgact attttacgct tatgggtctt atagcaggaa gcacaaccga tccacccgcg
     2881 cttgcttatg caaattcagc ttctgataac gatttacctg cggtaagtta tgctacagtc
     2941 tatcctctta caatgttttt gagagtcctc gcagcacaaa tattggtttt gttctgttaa
     3001 agtttatatc tttgcaaacc tttgcaaggc ctcatagttc aagggataga acggaagttt
     3061 cctaaacttc aaattcgcgt tcgagtcgcg gtggggctac cagtctgata ttattttctt
     3121 ggatttataa tgacagtgct gtcatttttg atctcatagg aaattggaga agctaaggac
     3181 atattattca gaatgtcaat tagagattct cctttgaaag ttcctgtata aatacatttt
     3241 tctgatagtt cagggcttac aataatttta actttatatt ttctttcaat ttttttaaca
     3301 aaggaaataa tgtcatcact ttctgccaca aaatagtttt ctgtccaggt ttgtcttcga
     3361 gtaatatttt tgacaattgt ttttttggta atcttatcaa atgtataaca gtaattgggt
     3421 tcaagtatta tatcttctga ttttgtgaga ttttttatct ctacctttcc actaaataga
     3481 gaggtagaga tctgcctgtc ttcattatat gcagataagt taaattcggt acctacaact
     3541 tcgacattta tgatatcagt tttgacaata aacttttttt cccccttttt tattttgaaa
     3601 tatccttcgc catccagata aacttctcgc tcgtttatgc catataatcc ggtatattta
     3661 agagtagaac cactgtttaa acttactgta gagccatctg gaagagttag gcaaacagat
     3721 tctccaagtg gtacagaaat tacactattt ttcagttctg atgtttcaat ccagtttgta
     3781 tactcatgtt tttttccaaa ataaaagcaa gagaaagcaa taatgagtat tgatgcaatt
     3841 tttagactcc atgagtatat cattcttaat ctttgccttt gaattaaatc acgttctctc
     3901 tgttcttctg agagaatttc tgaggcaaca ctcagatttt tcaactgagc aaagattttt
     3961 ttattctcct cattttgttc tatccattca acaatctcac gctgctcttt tggagaagcc
     4021 ttgccttcaa taaatcttaa taataaatcc tcttccattt attaatattc ctgacaaata
     4081 taaaaaacta tttattttta actatgatta cattatttcc attaataagc aaacctcttg
     4141 aaagatgttt atattgtttt gattcacaat ctattgctat gacttttcct gaataagtaa
     4201 tagtaatgtt attta
//
LOCUS       DAOTQL010000132         4165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16678_length_4165_cov_4.272506, whole genome shotgun sequence.
ACCESSION   DAOTQL010000132 DAOTQL010000000
VERSION     DAOTQL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16678_length_4165_cov_4.272506"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="P5293_07310"
     CDS             <1..966
                     /locus_tag="P5293_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49742.1"
                     /translation="DQKIQHTIHFITLSQMDKQYQIAVFYYTQTGQTLAILKSLLTPL
                     QSQGCQVIYKSIEPKIPFPYPWDKESFYQAFPESRQGIPCDLKPIDYSDIKDADLVVI
                     GLQPWFLSPSIPIASFLQHPDAIEYLNGKMVITVVGSRNMWVSCHDALSYYFMKTNSN
                     WIGNITLEDRHNNLVSVLTIFRWLIGGVKEKTRFLPSAGVSQEDIDKVSSLAPDILKT
                     LTDKDYDSLQQRIVEKGGVVFHYDLYQIEKVGYKMFGFWSKYVLRKGSFNSPDRSWRL
                     KLFSWYLMFVLFVLSPVAIAVFWLLFPLRKKSLSRIKKKYLYLCD"
     gene            978..2117
                     /locus_tag="P5293_07315"
     CDS             978..2117
                     /locus_tag="P5293_07315"
                     /inference="COORDINATES: protein motif:HMM:NF005293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRR49743.1"
                     /translation="MHPVYITKTSTFLPNEPVHNDEIEQYIGMIGGKPSRAKDIILRN
                     NGIKKRYYALDRQGNVTHTAYEMAVRAIEQLYDEDLNVETLELLAAGTTSQEMIMPSH
                     AAQIHGEMGGKRDMEVVSFAGSCCSGMHALKYAFLSLASGDKKNAVALASERLSAWMH
                     SSYFKKEYEDLQRLKEEPIIAFEKDFLRFMLSDGAGAFRLETKPAKNGLSFKINWIEL
                     TSFASTLPTCMYAGADYDATGKLRGWTYFPEDEWTTRTIFAIKQDTKLLGENIVKLGG
                     KFFMELVDKYSLKVGDVDWFLPHLSSMYFKPRIEKQLAEMNFNIPQERWFLNLPYVGN
                     VAAASAFLMLDELVRTKDLKSGQNILFMVPESAHFSYAYAFLTVC"
     gene            2120..2521
                     /locus_tag="P5293_07320"
     CDS             2120..2521
                     /locus_tag="P5293_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49744.1"
                     /translation="MKIEDVPQDGEILQSTLVRDVCYAIDQNGNYQEVISVGWEAKNE
                     ANNFAWIAINEESERIKEEVVAGRLSPLSYHLSRQVMTTEILAKYTGFSRREINKFCH
                     KPDIFFKLCYDDLHKLAAALNISVEQLISID"
     gene            2523..3533
                     /locus_tag="P5293_07325"
     CDS             2523..3533
                     /locus_tag="P5293_07325"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HRR49745.1"
                     /translation="MELEFEHKPAGHCENGVVRNLLKFYGVDLSEPMIFGLGSGIFFS
                     HMPFYKMHGMPVTSFRPLPGEIFSRVTKLLGIKVKRYKFRNQKLAMDKLDEILFQGIP
                     VGVLVGVYHLPYFPREYRFHFNAHNICVIGKEGDDYIVSDPVVIDKCRLSSNALRIVR
                     FAKGTYPPMGRMYYIKSIEKIRPLDANMIKKAILRACNRMLRIPFPMFGVNGIDYLSK
                     RVRRWEKIYGEKGAALNLAQVIRMLEEIGTGGAGFRFMYAAFLQEASEILNNSELKQL
                     SFELTEAGDMWRDFAYNSARFFKKREGEIETYDQIADKLAAIARKEKDIFTKLEKTVK
                     CG"
     gene            3526..>4165
                     /locus_tag="P5293_07330"
     CDS             3526..>4165
                     /locus_tag="P5293_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR49746.1"
                     /translation="MDNGLAIETVNLRKRYKGKSEFTLNDLSLKIPYGTLYGLLAPNG
                     AGKTTAINIICGLRRFDEGEVYVAGYSVKTDMKHIKPLIGLVPQDLALFPSLTARENL
                     KIFGGLYGLKGSILSKRVDELLELFDLTSSCKTIINHFSGGMKRRVNLIIGMLHSPEI
                     LILDEPTVGVDAQSRKLILDKLVEINRNGTTILFISHYLEEAEDICSEVALMD"
BASE COUNT         1326 a          688 c          876 g         1275 t
ORIGIN      
        1 gatcagaaga tacaacatac tattcatttt ataacattgt cgcaaatgga taaacaatat
       61 caaatagctg tcttttacta tactcagaca gggcagacac ttgcaatact caagagtctg
      121 cttactccat tacaatctca agggtgtcaa gtaatatata agagcatcga accaaagata
      181 ccttttccat atccttggga taaagaatct ttttatcaag cttttcctga gagtcgacaa
      241 ggtataccct gtgatttaaa acctatcgat tattctgaca taaaagatgc agatcttgtt
      301 gttataggac tccaaccttg gttcctatca ccatcaattc caattgcttc atttttacag
      361 catcctgatg caatagaata tttaaacggt aaaatggtta taacagtagt ggggtcaaga
      421 aatatgtggg tttcgtgtca cgacgctctt tcttactatt ttatgaaaac caattccaat
      481 tggataggga atattacact tgaagacaga cacaataatc ttgtaagtgt tttaaccatt
      541 ttcagatggc tgataggtgg agttaaagag aaaaccaggt ttcttccttc tgcaggtgtt
      601 tcacaggaag acatagacaa agtgtcatct ctggcacctg atattctaaa gacattaaca
      661 gataaagact atgattcact tcagcaaaga attgtagaga aaggcggagt agtatttcac
      721 tacgatctgt atcagattga aaaagtcgga tataaaatgt tcggattttg gtctaaatat
      781 gtgttgagaa agggcagttt taacagtcct gacagatcct ggaggttgaa gctattcagt
      841 tggtatctta tgtttgtgtt atttgtccta tctcctgttg ccatagctgt tttctggttg
      901 ctttttcctt tgagaaagaa gagcctaagt cgcattaaga aaaaatattt atatttgtgc
      961 gattaaccaa tcttaatatg catcctgtct atataactaa aacttcaact tttcttccca
     1021 acgaacctgt acataatgac gaaatagaac aatatattgg aatgattgga ggtaaaccct
     1081 ccagagccaa agatattatt ctaagaaaca atggcataaa aaaacgatat tatgctcttg
     1141 atagacaggg caatgtgact catactgctt atgaaatggc agtaagggca attgaacagc
     1201 tttatgatga agatttgaat gttgaaacgc tggaacttct tgcagctggc acaacttcac
     1261 aggaaatgat catgccgtct catgccgctc agattcatgg agaaatggga ggcaaaagag
     1321 atatggaagt agtatctttt gcaggttcct gttgtagtgg catgcatgct ctgaaatatg
     1381 ccttcctttc acttgcatca ggagacaaaa aaaatgctgt cgcacttgct tcagagagac
     1441 tgtctgcctg gatgcattca agttatttca agaaggagta tgaagatctt cagagattga
     1501 aagaagagcc gatcattgca tttgaaaaag attttctgag gtttatgctt tctgatggtg
     1561 ctggtgcatt tcgtttagag acaaaaccag caaaaaatgg tctttcattc aaaatcaact
     1621 ggattgaact gacttctttt gccagcactc ttccaacatg tatgtatgcc ggagctgatt
     1681 atgacgcaac tggaaaactg agaggatgga catattttcc tgaggacgag tggacaacca
     1741 gaacaatttt tgctatcaaa caggacacta aattgctggg tgaaaatata gttaaattgg
     1801 gcggcaaatt tttcatggaa cttgttgata aatatagttt aaaagttgga gatgttgact
     1861 ggtttcttcc tcatctttca tcaatgtatt ttaagcctcg catagaaaaa caacttgcag
     1921 aaatgaattt taatattcca caggagcgat ggtttcttaa tttgccttat gtgggcaatg
     1981 tagctgctgc ttctgctttc cttatgcttg atgaattagt aagaaccaaa gatcttaaaa
     2041 gtggtcagaa tattttgttt atggttcctg aaagcgctca tttttcttat gcatatgctt
     2101 tcttaacagt ctgttaaata tgaaaataga agatgtacca caggatggag agatacttca
     2161 gagcactctg gtaagggatg tttgttatgc catagaccaa aacggcaatt atcaggaagt
     2221 cattagtgtg ggatgggaag ctaaaaatga agcgaataat tttgcctgga ttgctataaa
     2281 tgaggaaagt gaaaggatta aggaggaggt tgtcgccgga agattgagcc ctttgtccta
     2341 tcatttaagc agacaggtta tgacaacgga aattcttgca aaatatacag gtttttcaag
     2401 gcgagagata aacaaattct gtcataaacc agatatattt tttaaactgt gctatgatga
     2461 tcttcataaa ctggctgcag cacttaacat atcagtggaa caacttatct ctatagattg
     2521 aaatggaact tgaatttgaa cacaaaccgg caggtcattg tgagaatggt gtggttagga
     2581 acctgctgaa attttatggg gtggatctat ctgagccgat gatttttgga ctgggttcag
     2641 gcattttttt ctctcatatg cctttttaca aaatgcacgg gatgcccgtt acttctttta
     2701 gacctctccc tggagaaatt ttttcaagag taacgaaatt actgggtata aaagttaaaa
     2761 gatataaatt cagaaatcaa aagcttgcaa tggataaact cgatgaaata ctttttcagg
     2821 gtattcctgt aggagttctt gtcggagttt atcatcttcc ttattttccc agagagtaca
     2881 ggtttcattt taatgctcac aatatatgtg ttataggcaa ggagggagat gattatatag
     2941 tgtcagatcc cgtagttatt gataaatgca gactctcttc caatgcactt agaattgtga
     3001 gatttgcgaa agggacatat cctcctatgg gaagaatgta ctatatcaag agcatcgaaa
     3061 aaatacgtcc tctcgatgcc aatatgatta aaaaggcgat ccttagagct tgcaacagaa
     3121 tgctccgaat accttttcca atgtttggtg ttaatggtat agactatctt tcaaaacgcg
     3181 taagaagatg ggagaagatc tatggtgaaa agggagcagc gctcaacctg gcacaggtca
     3241 ttaggatgct tgaggaaatt ggtacaggag gagccgggtt tagatttatg tatgctgctt
     3301 ttcttcagga agcttcagaa atactgaata attctgaact taagcaacta tcttttgaac
     3361 taacagaagc tggagacatg tggcgtgact ttgcttataa ttcggcccgt ttctttaaaa
     3421 agagagaagg cgaaatagaa acatatgatc aaattgctga caaattggct gcaatagcca
     3481 gaaaagagaa agacatcttc actaaactcg aaaagaccgt gaagtgtgga taatggatta
     3541 gctatagaaa ctgttaatct cagaaagaga tataagggta agtctgaatt tactctcaat
     3601 gatctgtcac ttaaaatacc atatggcact ctttatgggc tgcttgctcc aaatggcgca
     3661 ggcaagacta ccgctatcaa tataatatgt gggttaagaa gatttgatga aggagaagta
     3721 tatgttgcgg gttattctgt aaagacagac atgaagcata taaaaccgct catcggtctg
     3781 gttccacagg accttgcact ttttccttcc cttacggcaa gagaaaattt gaaaattttc
     3841 ggaggattat atggtcttaa aggctctata ttgagtaaaa gggtggatga attgctagaa
     3901 ctatttgacc ttacttcgag ttgtaaaact atcatcaatc acttttctgg tggaatgaaa
     3961 cgaagagtaa atcttattat cggaatgttg cattcacctg aaattcttat tcttgatgag
     4021 cctactgttg gtgtagatgc ccaatcaaga aaattgattt tggataagct tgttgaaatt
     4081 aacagaaatg ggacgacaat tctttttatc tcgcattacc ttgaagaggc ggaagatatt
     4141 tgcagtgagg ttgcgctaat ggata
//
LOCUS       DAOTQL010000133         4157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16707_length_4157_cov_5.437591, whole genome shotgun sequence.
ACCESSION   DAOTQL010000133 DAOTQL010000000
VERSION     DAOTQL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16707_length_4157_cov_5.437591"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="P5293_07335"
     CDS             complement(<1..645)
                     /locus_tag="P5293_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49747.1"
                     /translation="MREIRIVTAIALTFMLSAQLSFAQFYNIGNDPSHIQWREINTGE
                     FRIIYPQGIDSLAQRYAFLMSSAKARVEEKLKADIKPFPIILHPYTVMSNGVVAWAPE
                     RMELMTRPLPFRGYSQNWEKQLVIHETRHVAQMSKFEIGAFRPLNKLFGEQIAGLAAG
                     VYLGKWALEGDAVVSETEFSRSGRGRDPEQMIYFKASFLDGDYRNWPKWKLGSGR"
     gene            722..1594
                     /locus_tag="P5293_07340"
     CDS             722..1594
                     /locus_tag="P5293_07340"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HRR49748.1"
                     /translation="MKKLLLILILMLEAFGINAQNDSLAIVNTEWHQKQVAEGVLYKY
                     HHFSHNDLFNSNQFISIIEVAKTSRNKIEIYPSPVLKETSIMASEKGATAAINGSFFK
                     FSDTLNTKNYNSVDYIRKDGKILAYNLLEPKTIRLMHQLGALAIHRGELYILKADELR
                     NWEKYILAEDVLTSGPILRVAGWDESLEKSSFYTTRHPRTAVGKKANGNILLITIDGR
                     TKEAAGMSLDELQKILKWLGCSYAINLDGGGSTTMYIKGFSDNGIVNHPCDNKIFDNK
                     GERKVANALLLFCE"
     gene            complement(2600..2670)
                     /locus_tag="P5293_07345"
     tRNA            complement(2600..2670)
                     /locus_tag="P5293_07345"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2636..2638),aa:Gln,seq:ctg)
     gene            complement(2712..2782)
                     /locus_tag="P5293_07350"
     tRNA            complement(2712..2782)
                     /locus_tag="P5293_07350"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2748..2750),aa:Gln,seq:ttg)
     gene            complement(2837..3304)
                     /locus_tag="P5293_07355"
     CDS             complement(2837..3304)
                     /locus_tag="P5293_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HRR49749.1"
                     /translation="MELLSQLGLVGLFLGCFLAATIVPFSSDFLMVGILIAGVNPVLA
                     LIVATSGNWLGGLTTYYIGRIGKWNWIEKWFKVKEETLLKHKKNVDRYGSLLAFMTWL
                     PVVGDVFALALGFYRINFTKSALFMLIGKGLRFVFWTLLYYYVGEHVLDFKIL"
     gene            complement(3312..3746)
                     /locus_tag="P5293_07360"
     CDS             complement(3312..3746)
                     /locus_tag="P5293_07360"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRR49750.1"
                     /translation="MTQDELFMSEALKEARIAFDEDEVPVGAVIVTNGKIIARAHNLI
                     ERLNDPTAHAEMQAITMASNSFGGKYLITCTLYVTIEPCPMCAAALNWAQLPKLVYGA
                     SDPKHGYTLFSPSLLHPKCEVIQGVLSEECRNLMKSFFKSKR"
BASE COUNT         1431 a          829 c          703 g         1194 t
ORIGIN      
        1 tctccccgag cccagtttcc atttaggcca atttctataa tctccatcaa gaaacgatgc
       61 cttgaaataa atcatttgtt caggatctct tcctcttcct gatcttgaaa actctgtttc
      121 agaaacaaca gcatcacctt caagtgccca tttacccaaa taaactccgg ctgccaaacc
      181 ggcaatttgc tctccaaaca atttgttaag tgggcgaaat gctccaatct caaatttgga
      241 catctgtgct acatgccttg tttcatgaat tacaagctgt ttttcccagt tctgggaata
      301 acctctaaaa ggaagtggtc ttgtcataag ttccatcctt tcaggcgccc atgcaactac
      361 tccattgctc attacagtat aaggatgtaa tattatcgga aatggtttaa tgtctgcttt
      421 aagcttctct tccacacgtg ctttagcact gctcataaga aaagcatatc tttgtgcaag
      481 agaatcaatg ccttgtggat aaataatcct aaactcgccc gtattaattt ccctccattg
      541 tatatgagaa gggtcattgc caatattgta gaattgagca aaagataatt gtgcagacaa
      601 catgaaagtc agtgcaattg cagtaacaat gcgaatttct ctcataaaaa aattcctgtt
      661 acgaagataa ttaattttat gctctatata tttgtgtaac aaatcagcta taaatcagat
      721 tatgaaaaaa cttctattga tattaatctt gatgctggag gctttcggca tcaatgccca
      781 aaatgattcg ctagcaatag taaatacaga gtggcaccaa aaacaagttg cagaaggagt
      841 attatataaa tatcatcact tctcgcacaa cgatctcttt aattcaaacc agttcatatc
      901 aattatcgag gttgcaaaaa cttccagaaa caagatcgag atttacccct cacctgtgct
      961 taaagagaca agtatcatgg cttccgaaaa aggggcaaca gcggctatca atggttcttt
     1021 ttttaagttt agcgacacat taaatacaaa aaattacaat tctgtagatt acataagaaa
     1081 agacgggaaa atactagctt acaacctttt ggaaccaaag acaataagac tcatgcatca
     1141 gcttggtgca cttgccattc atagaggaga actttatatt ctaaaagctg atgaacttag
     1201 aaactgggaa aaatatatat tggcagaaga tgtacttact tcaggaccaa ttttgagagt
     1261 ggccggatgg gatgaatctc ttgaaaaatc ttctttttac accacaaggc atcctagaac
     1321 agctgtcggt aaaaaggcaa atggaaatat attattaata actatcgacg gcagaacaaa
     1381 agaagctgca ggcatgagcc ttgacgaact gcaaaaaata ctcaaatggc ttggctgcag
     1441 ctacgcaatc aaccttgacg gaggaggttc cacaactatg tacataaaag ggttctctga
     1501 taatggaatt gttaatcatc cgtgtgacaa caaaatattc gacaataaag gagaaagaaa
     1561 ggttgcaaat gcactattgc tgttttgcga ataattaaag atgaaaaaac aaaatgcact
     1621 ttttatttat acacaccttt caactttcgt taaaggagat atagaaattt tatctgaaat
     1681 ctgctctgtg aaaacatttc gggttgataa ttcttcacgg gtaaaacagt ttttttccct
     1741 catatggcaa atgctctatc tcattcttaa catatggaaa tttgatataa tttatatctg
     1801 gtttgcagat tatcactcat ttcttcctgt aatgttgtct aaaataacaa aaagaaggtc
     1861 attcctggtg attggcggat atgacgtatg ccgaataaaa agtgtaaaat atggcagttt
     1921 tattaatccg ataagaggct tcatgacgag atattcgatg aacaatgcat ccgtttgtct
     1981 ctgtgtttca aattatatag atagagtagt tcggttcata tcgaagaaag cgaagacaca
     2041 agttgtctac aactgtgtaa atattccaga tgacataagc gacaatacta ttacaaacag
     2101 agaaaaaggt gtaatttccg ttgcgatagc caaatctcca caaagtattt ttataaaagg
     2161 aatagacaga attgtcagac tatcacaaga gttaaaaaat atacctttta cacttatagg
     2221 tactgacaga ataatactgg aatccatctg tggctctata ccgcagaatc ttaaaataat
     2281 ttcccgaatc cctcacgaag aacttctgga atattacaaa aagtataaag tttactgcca
     2341 gctatccaga agtgaaagct ttgcgattgc tcttgcagaa gcgatgtact acaattgtgt
     2401 tcctgttgtt tcaaatactg gaggaatgac agaagtaact ggttccctcg ggtatatagt
     2461 tgatggagat aatattaatc aatgttcgga agcgattctt tctgctcttg atgagcctta
     2521 ttccgaaaaa tatcgtcaaa gaattgaaag taatttctca ataaaaaggc gttacgacgc
     2581 ccttaagaga atcttgtcgt tgacctacca ggattcgaac ctagatcgac agagccagaa
     2641 tctgtagtgc taccattaca ccataggtca atatatttaa aataaatcaa ttacagcatc
     2701 ccgttcttaa gttgacctac caggattcga acctggactg acagaaccaa aatctgtagt
     2761 gctaccatta caccataggt caattgtgta atttttgagc cgacaaatat atggcttttt
     2821 ttattaagtc caaaatttaa agaatcttga aatccaatac atgttcacct acataataat
     2881 acaaaagtgt ccagaaaaca aatcttaaac ccttaccaat caacataaag agagcactct
     2941 ttgtaaagtt aatcctataa aacccaagtg ccaaagcaaa aacatcacct acaacaggca
     3001 accatgtcat aaacgcaagc aaggaaccat acctgtcaac atttttttta tgttttagaa
     3061 gtgtttcctc ttttacttta aaccatttct caatccaatt ccatttgcct atacgaccaa
     3121 tataataggt tgtaagcccg cctaaccaat tcccggaagt tgccacaata agtgctaaaa
     3181 ctgggttgac accagctata agtatcccca ccatcaaaaa gtcagaactg aatggcacta
     3241 ttgtagcagc aaggaaacag cccagaaaaa ggccaacaag tcctaattgt gacaataatt
     3301 ccattacaga tctatctttt agatttaaag aaactcttca tcaaatttct acactcctct
     3361 gacaaaacac cttgaataac ctcgcatttt ggatgaagaa gcgaaggaga aaaaagagta
     3421 tagccgtgtt tgggatcaga agcaccatat accaattttg gcaattgagc ccagttaaga
     3481 gctgcggcac acattggaca aggttcgata gtaacatata aagtacaagt aataagatac
     3541 ttacccccaa agctattgga tgccattgta attgcctgca tctcggcatg tgcagttgga
     3601 tcattcagcc tttcgatcaa attatgagcc cgggcaataa ttttaccatt agttacaatg
     3661 actgcaccta cgggaacttc gtcctcatcg aaagctatcc gggcctcttt aagggcttcg
     3721 gacataaata actcatcctg ggtcataaac gcaaagttaa aataaaatca tacatactgc
     3781 tgcacctgca acataccata gaagggcaag aggaattttc tccattccca gtactgtaac
     3841 gccggataca gatccaataa tcattatgct tcctcatgta actgctcaat atgcaagaaa
     3901 cgtccaaaat gatccatcaa ccataaaaaa actgctgcag ccaaaggata cgaaagctat
     3961 tgcaataaca cctgcaataa acaaaaacat tgttgaactc atatgaataa ctaatgaaaa
     4021 aattgtggca attgtattac atttttaaga ctattattct cacttctcaa tttcaggcca
     4081 aattgacatt ttgtcagcag ggtcttctgg cacttatttt gctttattgt cattcgtcga
     4141 caataatttt aacatca
//
LOCUS       DAOTQL010000134         4124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16858_length_4124_cov_5.619071, whole genome shotgun sequence.
ACCESSION   DAOTQL010000134 DAOTQL010000000
VERSION     DAOTQL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16858_length_4124_cov_5.619071"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="P5293_07365"
     CDS             <1..739
                     /locus_tag="P5293_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRR49751.1"
                     /translation="ETADVGDLVQTIIVDSTVCARLKRSEVIDNANIKAGDVIVGLAS
                     FGKAVYEEEYNGGIGSNGLTSARHDLFSSYVGQKYPETYNNQIPKELAYTGSKRLDDI
                     EPETGLTYGKLLLSPTRTYAPVVKKMLESYRDKIHGMVHCTGGAQTKVLNFVDNVNVV
                     KNNLFSVPPLFRAIQSESKTPWKEMYKVFNMGHRLEIYTDLATALKLIEISESFGVEA
                     KVIGKVEQSRQTQVKIITEYGEFTYEK"
     gene            729..2126
                     /locus_tag="P5293_07370"
     CDS             729..2126
                     /locus_tag="P5293_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49752.1"
                     /translation="MRSKRNIVFFFTVILAVACSPKKVANNTYQSIPIIEKALRDTSS
                     VYFADYGSYPSDLKQLPIGIFDSGTGGLTVLEAFLSMDEFNNESFRYLADQANMPYGN
                     YAAANKLDYFRELVVKDALFLTTEPNHSKIIVIACNTATAYGLKDVQYLLEKGVPGVS
                     VIGVIDAGVNASLDKVRFDGNVAIGVMATVGTISSGGYQKTIKSSALKRGFKDEIKVV
                     CQPGMGFAEAVDMEVDYIDKNAVAIRDNYRGPVIGNDSISIDTTLLDRYRFNFANNSM
                     LYKKSGNRYVTIQLNSAANYARLYLVNLIERHRLRDGETPIDNIILGCTHYPFLLDTL
                     NKVVSELRDYTKDGDKIYDKILPNDFEFIDPSIYTAREVYQKLKDRKLLSDTYPANKL
                     KAFITVPAATVSPENIDKNGNFTYQFKYGREAGSEIQTVCTVPFSIQNLNPDNLLRIE
                     ERLPHTWHFLKEELK"
     gene            2140..2766
                     /locus_tag="P5293_07375"
     CDS             2140..2766
                     /locus_tag="P5293_07375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRR49753.1"
                     /translation="MIYAPTISKEELEILPQASFDGEIGIIDKKGEKYDKAIAYLSSQ
                     RIIGFDTETKPIFQANVKKNSVALLQLAGKDKAFIFRLHHLGLTDDLCKILSSNKIIK
                     VGVAVHDDIRGLQRHCKFVPKGFVDLQAVGINWGIREKSVRKMAAIILGVKVSKSQQL
                     SNWETDELSRAQVLYAATDAWICQRMYLKLLSTPKSPRIEEDEQIKNG"
     gene            2759..3958
                     /locus_tag="P5293_07380"
     CDS             2759..3958
                     /locus_tag="P5293_07380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRR49754.1"
                     /translation="MVKIFLKRSREESLERFHPWIFSGAIQRIEGSPAEGELVAVYSF
                     DGKLLGTGHYQSGSIAVRILEFGTDKLSDRFWIEKLLASYNFRRKLGLTESSTTNAYR
                     LVHGEGDMLPGLVIDIYDDTAVLQAHSAGIYLVRNQISDALLELYGGKLRAVYDKSSG
                     TVPSREIFNPVDAYLFGEKATPATILENGKIFNVDWCHGQKTGFFLDQRDNRALMEHY
                     AKNRNVLNLFCYTGGFSIYALAAESTSVASVDSSRSAVELLEKNVELNRQSGIISKEA
                     LHKSFCTDAVEYMKSEGKEIYDLIVVDPPAFAKHKSALSNALRAYQRLNAAAIDKVAH
                     GGIIFTFSCSQVVDKISFAQTIFSAAAETGRAVRILHRLAQAPDHPVNIYHPEGEYLK
                     GLVLFVE"
BASE COUNT         1333 a          684 c          884 g         1223 t
ORIGIN      
        1 agagactgct gatgttggag atcttgtgca gacaataatt gttgacagta ctgtttgtgc
       61 aagactgaaa agaagtgaag ttattgataa tgccaacatt aaagccggag acgtgattgt
      121 ggggcttgcc tcatttggaa aagctgttta tgaagaggag tataatggtg gaataggcag
      181 caacggactt acttctgcaa gacatgatct tttttcttct tatgttggac aaaaatatcc
      241 cgagacttat aataaccaga ttccaaagga gttggcttat actggttcca aaagattaga
      301 tgatattgaa cccgaaacag gccttactta tggcaaatta cttctttctc ctacaagaac
      361 atatgctcct gttgtcaaaa aaatgcttga aagttacagg gataaaatac atggtatggt
      421 ccactgtaca ggaggggctc agactaaagt attgaatttt gttgacaatg tcaacgttgt
      481 taagaacaat cttttttctg ttcctcctct ttttagagcg attcagagtg aatccaaaac
      541 accttggaag gagatgtaca aagtatttaa tatggggcac agacttgaga tatacactga
      601 tctggctaca gctttgaaac tgattgaaat ttcagagagc tttggagttg aagcaaaagt
      661 aattggtaag gtggagcagt caagacagac tcaagtaaag ataattactg aatatggaga
      721 attcacatat gagaagtaaa agaaacatag tatttttttt tacggttatc ttagcagtag
      781 cttgttctcc taagaaagtt gcaaataaca catatcaaag cattccaatt attgagaaag
      841 ctttaagaga tacctcttca gtctattttg ctgactatgg tagttatcct tctgatttga
      901 aacagctccc gattggtatt tttgattcag gaacaggggg attgactgtt cttgaagcct
      961 ttctatcaat ggatgaattt aataatgagt cgtttagata tcttgcggat caggcaaata
     1021 tgccatatgg caattatgct gctgcaaaca aattggacta tttcagagag cttgttgtga
     1081 aggatgcact tttccttacc acagagccta atcattccaa gataattgtt atagcgtgta
     1141 acacagcgac agcttatggt ttgaaagatg ttcaatatct tttggaaaaa ggcgtgcctg
     1201 gggtatcagt tatcggagtg atagatgcag gtgtaaatgc ttctctcgat aaggtcagat
     1261 ttgatggaaa tgttgcaata ggagtaatgg ctactgttgg tacaatttct tcaggaggat
     1321 atcagaaaac aattaaatct tctgctttaa aaagagggtt taaagatgaa ataaaagttg
     1381 tgtgtcaacc agggatgggt tttgctgagg ctgttgacat ggaggttgat tatattgata
     1441 aaaatgctgt cgccatccgt gataactata gaggccctgt tataggaaat gattcaattt
     1501 ccatagatac tactttgctt gacaggtaca ggtttaattt tgccaacaac tcgatgctct
     1561 ataaaaaaag tggaaacaga tatgtgacca tacagcttaa ttctgcggca aattatgcaa
     1621 gattatactt agtcaatctt atagagagac acagattgag agatggagaa acacctatcg
     1681 acaatataat cctgggctgt actcattatc catttttgct tgatactctc aacaaagtgg
     1741 tttcggaact tcgggattat acaaaagacg gagataagat ttatgataag attttaccaa
     1801 acgattttga atttattgat ccgtctattt atacagccag agaagtttat caaaaattaa
     1861 aagaccggaa gcttctttct gacacatatc ctgcaaataa attaaaagca tttataacag
     1921 ttcctgcggc aacggtaagt cctgaaaata tagataaaaa cggaaatttc acatatcagt
     1981 ttaagtatgg aagagaagca ggaagtgaaa ttcaaacagt ttgcacagtt cctttttcaa
     2041 tacaaaatct taatccagac aatcttctca gaattgagga gagattgcct catacatggc
     2101 attttttgaa ggaggagctt aaatgataat ttttaactga tgatttacgc tccgacaata
     2161 tctaaagagg agttggaaat actccctcag gcatcatttg atggagagat aggtataatt
     2221 gacaaaaaag gagagaaata tgacaaggca attgcttacc tctcatctca gaggattatt
     2281 ggatttgata ccgaaacaaa gccaatattt caggccaatg taaaaaaaaa cagtgttgct
     2341 ctattacaat tggcaggcaa agataaagct ttcatcttca ggcttcacca tctcggtctt
     2401 acagatgatc tctgtaaaat actgtcttca aacaaaatca taaaagttgg tgtggcagtt
     2461 cacgacgata tcagaggatt gcaacgtcat tgtaaatttg ttcctaaggg ttttgtagat
     2521 ctgcaggcgg tagggataaa ctggggcatc agagagaaaa gtgtccgcaa aatggctgca
     2581 ataatacttg gggttaaggt atccaagtca cagcagcttt ctaactggga aacagatgaa
     2641 ctttcacgtg cacaagtttt gtatgcagct acagatgcct ggatttgcca aagaatgtat
     2701 cttaagcttt taagtacgcc taaatctcca agaattgaag aagacgagca gataaaaaat
     2761 ggttaaaatt ttcttaaaga gatcgagaga agagtcgctt gagagatttc atccatggat
     2821 tttttcaggt gccattcaaa ggatcgaagg cagtcctgct gaaggagaac ttgttgcagt
     2881 gtattctttt gacggaaaac ttcttgggac aggtcattat cagagtggtt cgattgcagt
     2941 tagaatcctt gagtttggta ctgacaaact atctgataga ttttggattg agaaactttt
     3001 ggcttcatat aatttcagaa ggaaactggg tcttacagaa agctctacta ctaatgcata
     3061 tagacttgtt cacggcgaag gagacatgct cccaggcctt gttattgata tttacgatga
     3121 tactgcagtg ttacaggctc attctgcagg tatttatctt gtcagaaatc agatatctga
     3181 tgcactatta gaactttatg gcgggaaatt aagagctgtt tacgataaaa gttcaggcac
     3241 agtgccgtca agagaaattt tcaatcctgt tgatgcttat ctctttggtg aaaaagctac
     3301 tcctgcaact attttggaaa acggaaagat attcaatgtt gactggtgcc atggtcagaa
     3361 aacaggcttt tttctcgatc agagagacaa cagagccttg atggaacatt atgcaaaaaa
     3421 cagaaatgta ctaaatcttt tctgttatac cggcgggttt tcaatctacg ctcttgcagc
     3481 cgaatccact tcggttgctt ctgtcgacag ttctcgtagt gccgtagagc tgcttgaaaa
     3541 aaacgtagaa ttaaacagac aatcaggtat tatatccaaa gaagctttgc ataaatcttt
     3601 ttgcacagat gctgttgaat atatgaaaag tgaggggaag gaaatatatg atttgatagt
     3661 agttgatcca cctgcatttg ctaaacacaa aagtgcactt tcaaatgcgc ttagagccta
     3721 tcagcgactt aatgccgctg caatagacaa agttgcacac ggaggcataa tatttacatt
     3781 cagctgctca caagttgttg acaagatatc ttttgctcaa actatatttt ctgcagcagc
     3841 tgagactgga agggcagtaa ggatactgca cagacttgca caagctcccg atcatcctgt
     3901 aaacatttat catcctgaag gagaatacct caagggatta gtcctctttg ttgagtaatt
     3961 atatatcttt gtcttgttat ggaacaattg gagataaaag aagcgagcaa ggaggagatg
     4021 tataagttgc ttcttcctca agtggcgtca ttgaccgagg gagaggagga cattattgcc
     4081 aacatggcca atgtggcttc agcaataatg atgaccttta aaca
//
LOCUS       DAOTQL010000135         4100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_16975_length_4100_cov_4.355501, whole genome shotgun sequence.
ACCESSION   DAOTQL010000135 DAOTQL010000000
VERSION     DAOTQL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16975_length_4100_cov_4.355501"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1408)
                     /locus_tag="P5293_07385"
     CDS             complement(47..1408)
                     /locus_tag="P5293_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRR49755.1"
                     /translation="MDLFANFIKNLSDFLWGWPMILLLLGTHIYLTIVLKFPQRKIFT
                     GIKLSVAKDKGAEGDVSQFGALSTALAATIGTGNIVGVATAVALGGPGAVLWCWLTGV
                     FGIATKYSEGLLAIKYRVKTSDGTMIGGPMYALERGLKMKWLGVLFCIFAAVAAFGIG
                     NTVQANSISMLLNETYSISNYITGAVIAILTALVILFGVKGIAKVCSTLVPFMAFFYV
                     LGCLYILIMNYHFLGDSFRIIFESAFNARAAGAGFLGTTVMMAARYGIARGLFSNESG
                     LGSAPLVAAAAKTRNPVRQALVSSTGTFWDTVVICALTGLVIVSSILAFPDIDHTDGA
                     ALTKAAFSKIPFGSVILSVGILTFAFSTILGWSYYGEKAVEYLGGKKIIFWYRIAWII
                     AVFIGSVVNLSLVWNIADCMNALMAIPNLISLILLSGVLVKETNKYLWSGRLDETSID
                     EAG"
     gene            complement(1451..1948)
                     /gene="tpx"
                     /locus_tag="P5293_07390"
     CDS             complement(1451..1948)
                     /gene="tpx"
                     /locus_tag="P5293_07390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HRR49756.1"
                     /translation="MFAAKGTPLTLLGKQIEVGDVAPEINAVDASMNEVKLSDYKGKV
                     VVISVFPSIDTPVCATQTRTFNKKATELSNDVVILSISKDLPFALGRFCAAEGINNVH
                     TLSDYKYSKFGLEYGFLIKETMLLARGVLVIDRNGVVRYKEITRDIVNEPDYDKAFEA
                     VKKLL"
     assembly_gap    1981..2080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2081..3088
                     /locus_tag="P5293_07395"
     CDS             <2081..3088
                     /locus_tag="P5293_07395"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR49757.1"
                     /translation="VRYVFDNSPMSENGVKRGFELTHLNGTPVMDLIRNGTFNSKMNN
                     SSNSFTFKRYDGSSYTFSTSAREVNTKSVLKSMIITSADFPGLPYPVAYFNYYAFKAG
                     MTSEIDNAMATFKAANVKELILDLRYNGGGDAAATQLLSNYIAPASANGKIVARRKHN
                     DKLSAYDSDVNTMTVVQRVSNSLDLNRLIILTSGGTASASEIIVNGFKPLMNVLQVGR
                     TTYGKPNGMYVWFYPENEELYPDYVFLPVAFYSVNSLGEGDYENGIVPDQQRPDDLYH
                     DFGLEEDWLKSSLTFIATGSWPSVPAVLPTAAPQFSGAKIKIEEDSPNYGKYIDHKKP
                     L"
     gene            3085..>4100
                     /locus_tag="P5293_07400"
     CDS             3085..>4100
                     /locus_tag="P5293_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRR49758.1"
                     /translation="MNIRVKAKVIMVSLFLLPIMTIESKAQRDSIVSRIIEEGKSHNL
                     TMDHLDVLTNRIGGRVCGSDAYENATFWAANLFRKWGLNVIVQAVDTFPVGFNRGPWF
                     GRMLSEDGYVLHFATPSYTAGTKGVQRGHVVLEPKTKEEFNRMKGKLKGAWVLITGES
                     DGFPIDYSTFADEERHRIIKENEDIVKYNDSIKRWNDNNPRKEQKELKPLKDSPALFY
                     KEMREAGILGIIQSAKVPLRALYDKKNLFLMSFDSLPTCPDIKLDEEQYNNILKKVKE
                     RRYFQLEFDIRNHFKPGPVVVHNVIGEIKGSVYPDEYVMCGGHLDSYDVATGAVDCGS
                     GVAPT"
BASE COUNT         1318 a          831 c          736 g         1115 t
ORIGIN      
        1 agaatatgga aaaaaatata actttttgaa ctaaaagact cgtaaattaa cctgcctcat
       61 caattgaagt ttcatcaagt cttcccgacc agagatattt gttagtctct ttgacaagga
      121 cacctgaaag caagatgaga gatatcaagt ttggaattgc cataagagca ttcatacagt
      181 ctgctatatt ccaaacaagc gacaagttaa ccaccgaacc gataaagact gctattatcc
      241 acgctattct ataccaaaaa ataatttttt taccgccaag atattctacc gctttctcgc
      301 cataataact ccagcctaaa attgtactga aagcaaatgt aagtatcccg acagatagta
      361 ttacagaacc aaacgggatt ttagaaaatg cagctttagt caaagctgca ccatctgtat
      421 gatcaatatc gggaaatgca agaatagaac taacaattac cagccctgtc aaggcacaaa
      481 ttactacagt atcccaaaat gtacctgtgg aagataccag cgcctgtctg acagggtttc
      541 ttgttttggc tgctgctgca accaagggag cagaaccaag tcccgactca ttcgaaaaaa
      601 gacctctcgc aataccatat cttgctgcca tcatgacagt agtcccgaga aaacctgctc
      661 ctgcagctct cgcattaaaa gctgattcaa aaattattct gaaagaatct cctaaaaaat
      721 gataattcat tattaaaatg taaaggcatc ccaaaacata aaaaaatgcc atgaagggaa
      781 ccaatgtgct acaaacttta gcaattccct taactccaaa cagaataacc aatgcggtaa
      841 gaattgcaat tacagctcca gtaatgtaat tggagataga ataggtttca ttcaataaca
      901 ttgaaataga gttagcctgc actgtgttcc caataccaaa agctgcgaca gcagcaaaaa
      961 tacagaacag tacaccaagc catttcattt tgagccctct ctccaaagca tacattggtc
     1021 caccaatcat tgttccatcg gatgtcttaa ctctgtattt aatcgccaat aatccctccg
     1081 aatacttggt tgcaatccca aacacaccgg taagccagca ccacaaaaca gcaccaggac
     1141 cacccagagc cactgctgtg gccactccca caatgttccc ggtacctatt gtcgcagcta
     1201 aagctgttga caatgcacca aattgactca catcaccttc agcacccttg tctttagcaa
     1261 cagatagctt tatccctgta aaaatttttc tctgaggaaa ttttaataca attgttaaat
     1321 aaatatgggt gcctaataat aataatatca ttggccaccc ccaaagaaaa tcactgaggt
     1381 ttttaataaa atttgcgaat aaatccatgg tttaagtctt tattcgcaaa tataaaaata
     1441 tactgtcttt ttaaagcaat tttttcactg cttcgaaggc tttatcataa tccggctcat
     1501 tgacaatatc cctggtaatc tccttgtaac ggacaactcc gtttctgtct attacaagta
     1561 ctcctcttgc aagaagcatt gtctctttta tcagaaatcc atactcaaga ccaaatttgg
     1621 aatacttata atcagaaaga gtatgtacat tattgattcc ttcagctgca caaaatctgc
     1681 caagagcaaa tggcaaatcc tttgatatgg aaagaattac aacatcatta gatagctcgg
     1741 tggctttctt gttgaaagtt ctggtctgag ttgcacaaac aggtgtgtca atcgaaggaa
     1801 acacacttat tactaccacc ttacccttat aatcagatag ttttacctca ttcatcgagg
     1861 catctacagc attaatttca ggcgcaacat cacccacttc tatctgcttg ccaagcagtg
     1921 taagaggtgt gccctttgct gcaaaaatat tttttgtttc aatagataat aattcttgag
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttagatatg tttttgacaa
     2101 ctctccgatg tcagaaaacg gagtaaaaag aggatttgaa ttgactcatc ttaatggaac
     2161 tcctgtcatg gatctcatta gaaacggaac tttcaacagt aaaatgaata actcttccaa
     2221 ctcttttact tttaaaagat atgatggaag ctcctacacc ttctccacta gtgcaagaga
     2281 agtaaacacc aaatccgttt tgaaaagcat gattataact tcagctgatt tcccgggttt
     2341 accatatcca gtagcatact ttaactacta tgccttcaag gctggtatga catctgaaat
     2401 tgacaacgct atggctactt tcaaagctgc aaatgtcaaa gaacttattc tagatcttag
     2461 atataatgga ggaggagacg cagctgcaac tcaactgctt tcaaactata ttgcccctgc
     2521 aagtgccaac ggaaaaattg tggcaaggag aaaacataat gacaaactgt cggcttatga
     2581 ttctgacgtt aatacaatga cagttgttca aagggtttcc aattctcttg acctgaacag
     2641 actgattatt ttgacaagcg gtggtacagc ctccgcaagt gagatcatag ttaacgggtt
     2701 caagcctttg atgaatgtac ttcaagttgg tcgtacaact tatggtaaac cgaatggtat
     2761 gtatgtttgg ttttatcctg aaaatgaaga actctatccg gattacgtct tccttccagt
     2821 tgctttttac agcgttaaca gtcttggaga aggtgattat gagaacggga ttgtgccaga
     2881 ccagcaacgt ccggatgatc tatatcatga ttttggcctt gaagaagact ggcttaaatc
     2941 ctccctgacc tttattgcaa caggatcatg gccttcagtg ccggcagtac tgcccacagc
     3001 agcgccacag ttctctggtg ccaaaattaa aattgaagag gattctccta actatggcaa
     3061 atatatagac cataaaaaac cattatgaat attagagtaa aagccaaagt cattatggtg
     3121 tctctttttc tcctccctat aatgactatt gaatctaaag cccaaagaga cagtatagtc
     3181 tcacgtataa tcgaagaggg taaaagtcac aaccttacca tggatcatct cgacgtttta
     3241 accaatcgga ttggaggaag agtttgtggt tcagacgcat atgaaaatgc cacattttgg
     3301 gcagccaatc ttttccgtaa atggggtctg aatgtaattg ttcaggctgt tgacacattc
     3361 cctgtaggat ttaacagggg tccatggttt ggacgcatgc tgagtgaaga tgggtatgta
     3421 cttcattttg caacgccctc ctacacggca ggaaccaaag gcgtccaaag aggccatgtt
     3481 gttctggaac caaaaaccaa agaagaattt aacagaatga aaggaaagct aaaaggagcg
     3541 tgggttctta ttaccggaga aagcgacgga tttcctattg attactccac atttgcagat
     3601 gaggagagac acaggattat taaggaaaat gaagatatag taaaatataa tgactcaata
     3661 aaacgttgga acgataataa tcctcggaaa gaacaaaagg aacttaaacc tttaaaagat
     3721 tctccggcac tcttctacaa ggagatgaga gaggctggta tacttggaat tatacagtct
     3781 gccaaggtgc cactaagagc gctctacgac aagaaaaatc tttttctaat gagtttcgat
     3841 tctcttccaa catgcccaga tataaaactt gatgaagaac agtataataa tattctcaaa
     3901 aaagtaaagg aaaggagata ttttcaactt gaatttgata ttcgtaatca ttttaaacca
     3961 ggccctgttg tcgtacataa tgttatagga gagattaaag gaagcgtata tcctgatgag
     4021 tatgtaatgt gcggaggaca tcttgattcc tatgatgtcg ccacaggagc tgttgattgt
     4081 ggttcaggtg tagccccgac
//
LOCUS       DAOTQL010000136         4084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_17053_length_4084_cov_4.297841, whole genome shotgun sequence.
ACCESSION   DAOTQL010000136 DAOTQL010000000
VERSION     DAOTQL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17053_length_4084_cov_4.297841"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..982
                     /locus_tag="P5293_07405"
     CDS             2..982
                     /locus_tag="P5293_07405"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRR49759.1"
                     /translation="MNGIGAYLPEYILTNDELSKMVDTSDEWIMTRIGVKERRILKED
                     GLGTSHMGALAVEDLIKKTGLNRDEIDILICATVTPDMLFPATANIICDKVGIKNAWG
                     FDINAGCSGFIFSFANCASLIESGRAKKIVLVTGERMSAITDYTDRSTCPLFGDGAVA
                     MLIEPGDDPSVGLVDEILHVDGIGRQYLFQKAGGSLYPPTEQTLKNREHYIYQDGQVV
                     FKYAVSRMADVSVEMMEKHNLKASDIAYLVPHQANLRIIEATAKRMGLDKNKVLINIQ
                     KFGNTAGTSIPLVLWEFEKKFKKGDNLILSAFGAGFTWGALYYKWAYDPS"
     gene            complement(1005..2981)
                     /locus_tag="P5293_07410"
     CDS             complement(1005..2981)
                     /locus_tag="P5293_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HRR49760.1"
                     /translation="MRNLSQHEIDSLSANLCVAEDWTKVFVSERFSTDHIYNVRFSGR
                     VTLGCFEKTFSLPGGICRHSGIRNATIHNCQIGDNVFIENISNYISNYIIGECCFIQN
                     VDIIYVDGISTFGCNASIMVLNETGGREVPIYPRLSSSLAYIIALYRHKNGTIGKLTK
                     LIADYSKEHESSNGTIGKQVTIINTQTIKNVKIEDCAVIENVLRLENGTICSNEHAPV
                     IVKDGVIANDFIFASGSFIAEASKITRCFVGQACHISHSFSAHDSLLFANCNFENGEA
                     CAVFAGPFTVSVHKSSLLIAGLFSFLNAGSGSNQSNHMYKLGPIHQGIVERGSKTTSD
                     SYILWPAKIGAFSLVMGRHYRHPDTTDLPFSYLIENSDETFLVPGVNLKSVGTIRDVQ
                     KWPKRDKRTDKDLLDLINYNLLSPYTIQKMINGRKILLELRSVSGETSEIFTYRSSRI
                     KNSSLRNGIKLYEKAINKFFGNSLLRRLESTPFRDIYQIREQLKPTHPLGKGEWVDLS
                     GLIVPKSEVEKLLSEIDENRVNLNDIEERFRILHQDYYDMEWTWAYGAMEKYYGINLS
                     EISIEKLRSLVELWRESVVTLDEMLYNDAMKEFSITCMTGFGVDGSEDEKHCDFECVR
                     GEVESNPFVSTVIQHIKKKSELANEFMARVKHIS"
     gene            complement(3063..3755)
                     /locus_tag="P5293_07415"
     CDS             complement(3063..3755)
                     /locus_tag="P5293_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRR49761.1"
                     /translation="MKTLLLALTILSGFGGIKATQNQEKVYILPSPSLKGDVSVEEAL
                     QSRRSRRQFDDRELSTEQLSQILWSAYGITLKRDSNPNLRGGLRTAPSAGALYPLEIY
                     VFVGKVKGIDPGVYKYIPKDHSIVLVMDKDLRSELSAAALNQKMIKDAPTVLVYTSVL
                     ERSGKYGQRGKERYIFMDLGHSAENVYLQAEALKLGTCAVGAYSDEALKKLMQLPEGE
                     EPVYLMPVGYCK"
     gene            3904..>4084
                     /locus_tag="P5293_07420"
     CDS             3904..>4084
                     /locus_tag="P5293_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRR49762.1"
                     /translation="MEVNTGRSDLLWNYAASFMRLASALIILPLILHPLPVEEVGLWN
                     IMISLNAMIYLLDFGF"
BASE COUNT         1191 a          762 c          795 g         1336 t
ORIGIN      
        1 tattaatggc attggtgcat accttccaga gtatattctt acaaatgatg agttgtcaaa
       61 aatggttgac acaagcgatg agtggattat gactcgtatt ggcgtgaaag agagaaggat
      121 acttaaagaa gacggacttg gcacttccca tatgggagcg ttagccgtgg aagatctcat
      181 taaaaaaacg ggtctgaaca gagatgagat agatatattg atttgtgcaa ctgttactcc
      241 agatatgcta ttccctgcga ctgctaatat tatttgtgac aaagttggga ttaagaatgc
      301 atggggtttt gatattaatg caggctgttc aggctttata ttcagttttg caaattgtgc
      361 ttcacttatt gagtcaggcc gtgcaaaaaa aatagtgttg gttacagggg agagaatgtc
      421 tgctattaca gattacactg acagaagtac atgtcctctt tttggtgacg gtgctgttgc
      481 catgttgatt gaaccaggtg atgatccatc ggtcggactc gtagacgaaa tacttcatgt
      541 cgacgggatc ggcagacaat atctttttca aaaagccgga ggttcgcttt atccacctac
      601 ggaacagact ttaaaaaaca gggaacatta tatttatcag gatggtcagg tagtctttaa
      661 atatgcagtc agccgtatgg cagatgtttc tgtagagatg atggaaaagc ataatctcaa
      721 ggcgtcagat atagcctatc ttgtgcctca tcaggccaac ttgaggatta tagaggcaac
      781 tgctaaaaga atgggtttgg ataaaaacaa agttcttata aacattcaga aattcggtaa
      841 cactgctgga acatcgatac cgcttgtact gtgggaattt gagaaaaaat tcaaaaaagg
      901 agacaacctg atattatcag catttggtgc aggatttact tggggagcat tgtattacaa
      961 gtgggcttat gacccgtcat aattacaatt tgttgttttc ttaattagga tatatgtttc
     1021 actcttgcca taaattcatt ggcaagttca ctctttttct taatgtgttg aataactgtt
     1081 gacacgaaag gattgctctc aacttcccct cttacacatt caaagtcaca atgtttttca
     1141 tcttcgcttc cgtcaactcc aaagcctgtc atacatgtaa ttgaaaactc cttcatggca
     1201 tcgttgtaaa gcatctcgtc caatgttaca acagattctc tccaaagttc aacaagagat
     1261 ctcagctttt ctatcgatat ttccgagagg ttgataccat aatacttctc cattgcaccg
     1321 tatgcccaag tccattccat gtcatagtag tcttggtgaa ggattctgaa ccgctcttca
     1381 atgtcattta gattgacacg gttttcatca atttcagaca ataacttttc tacttcagac
     1441 ttagggacaa taagtccgga aagatctacc cactctcctt ttcccaaggg atgtgttggc
     1501 ttaagctgct cccgtatttg gtaaatatcc ctgaatggtg ttgattcaag tcttcttaga
     1561 agtgagtttc cgaaaaattt attgattgct ttttcataca gttttattcc atttctaagt
     1621 gaactgtttt taattcggga acttctgtaa gtaaatattt cagaagtttc tcctgacaca
     1681 gaacgtaatt cgaggagaat ttttctccca ttaatcattt tctgaatagt gtatggactt
     1741 aggagattgt aattgatcaa atcaagaaga tctttatctg ttcgtttgtc tctttttggc
     1801 catttctgga catctcttat ggtaccaacg cttttcagat taactcccgg aacaagaaat
     1861 gtttcatcag aattttctat aagataggag aaaggaaggt ctgtagtgtc gggatgcctg
     1921 taatgccttc ccataacgag tgaaaaggct ccaattttgg ccggccagag aatgtatgaa
     1981 tcacttgttg tttttgatcc tctttccaca attccctgat gaataggtcc aagtttatac
     2041 atatggttac tttggttgga accacttccg gcatttaaaa atgaaaaaag gccggcaata
     2101 aggaggctgg acttgtggac agaaacagta aatggtcccg caaatactgc gcaagcttca
     2161 ccattttcaa agttgcaatt tgcaaatagt aacgaatcat gagccgagaa actgtgtgaa
     2221 atgtggcagg cttgtccaac gaaacatctg gttattttag aagcctcagc aatgaatgat
     2281 ccggatgcaa agataaagtc attggctatt actccgtcct taacaatcac aggcgcatgt
     2341 tcattactgc aaattgtacc attttccaat ctaagtacat tctcaattac tgcacaatct
     2401 tcaattttga catttttaat agtttgtgta ttaataatgg ttacttgttt accaattgtt
     2461 ccgtttgaag actcatgttc tttggaataa tctgctatca gttttgttaa tttgcctatt
     2521 gtgccgtttt tatggcggta aagagcaata atgtaagcaa gagatgatga gagccgggga
     2581 taaataggaa cttctctgcc accagtctcg ttaagaacca ttatagaagc attacaacca
     2641 aaagttgata ttccgtctac ataaataatg tcaacatttt ggatgaagca gcactctcca
     2701 atgatataat ttgaaatata attggagata ttttcgataa acacattatc cccgatttga
     2761 caattgtgga tagttgcgtt tctgatacct gaatggcggc aaataccgcc tggaagggag
     2821 aatgttttct caaaacatcc caaagttacc ctgcccgaga aacgaacatt gtaaatgtga
     2881 tctgttgaga acctctccga cacaaagact tttgtccagt cttccgccac acaaagattg
     2941 gctgacagcg agtcaatctc gtgttgagat aaattcctca tttgttatgt ataatttttt
     3001 cgagcttgta actgtggtac ccttgcccga aatttgcagt ggcgaattta aggttttttc
     3061 ttttatttac aatatcccac aggcataaga tatacaggtt cctcgccttc gggtaattgc
     3121 attagttttt ttaaagcctc gtcagaataa gctcctactg cacatgttcc aagtttaaga
     3181 gcttcagcct gcagataaac attttctgca gagtggccaa gatccataaa gatatatctt
     3241 tcttttcccc tttgaccata ttttccactt ctttcgagta cagatgtgta aacaagcaca
     3301 gtaggtgcat ctttaatcat tttttgattt aatgcagctg cactcagttc acttcttaaa
     3361 tctttgtcca taacaagtac aatagagtga tcttttggta tatatttata tacccctggg
     3421 tcaatcccct tgacctttcc aacaaagaca taaatttcaa gcggatacag tgctcctgcg
     3481 gacggtgctg ttctcaaacc accgcgcaag ttgggattag aatccctttt tagggtaatt
     3541 ccgtaagcag accaaagaat ttgtgacaat tgttctgttg aaagctctct gtcatcaaac
     3601 tgtcttctag accttctgga ttgtaatgct tcttctacgc ttacatcacc ttttaatgaa
     3661 ggagagggca agatgtagac tttttcctga ttttgtgttg ctttaattcc accaaatcct
     3721 gacaaaatag ttaatgccaa tagtaatgtt ttcatattaa aatatttttt gaaaaataat
     3781 attaatttca gttataacaa aacgcgttta atatgaaatt ttaatggttt gtcggataaa
     3841 ttgattttca gttttgaata taatcttaca tttgcaaaac agtcgttttg caaatcatag
     3901 attatggaag taaacactgg aagatcagat ctgctttgga attacgcggc gagttttatg
     3961 aggcttgcgt ctgctctgat tatcctgcct ttgatactgc atcctttacc tgtagaggag
     4021 gtgggtcttt ggaatattat gataagcctt aatgcaatga tatatctgct tgatttcgga
     4081 tttt
//
LOCUS       DAOTQL010000137         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_17463_length_4005_cov_4.576962, whole genome shotgun sequence.
ACCESSION   DAOTQL010000137 DAOTQL010000000
VERSION     DAOTQL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17463_length_4005_cov_4.576962"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="P5293_07425"
     CDS             complement(<1..265)
                     /locus_tag="P5293_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRR49763.1"
                     /translation="MEHDHIQIHDNPGRAFKWGIILNVIFIAVEIIYGLLSNSMALIA
                     DAGHNFSDVLALVFSWIAVIISQRKPTLKFTYGFRRSTILIALL"
     gene            350..1072
                     /locus_tag="P5293_07430"
     CDS             350..1072
                     /locus_tag="P5293_07430"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRR49764.1"
                     /translation="MKHLFTILFFIATVPCLAVAQNNNKLSNNKNNINMKTISTLEFP
                     ELPYSTDALEPFIDKQTVEIHYGKHQKTYFDNFMAAVKGTDAENLTILELFKNISKYP
                     AAVKNNGGGFYNHVLYWESIKPNGGKPSAKLTDAINKSFGSLDELKKQFTDAGKNRFG
                     SGWAWLCVDEKGNLFVFSTANQESPLMDVAEKHGTPLIAMDVWEHAYYLKYQNKRADY
                     IEAFWQIINWEVVSQKYEALVK"
     gene            complement(1090..1992)
                     /locus_tag="P5293_07435"
     CDS             complement(1090..1992)
                     /locus_tag="P5293_07435"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRR49765.1"
                     /translation="MNRVCKLFETEFPIVQGGMIWCSGWRLAAAVSNAGALGLIGAGS
                     MHPDNLRYHIKSCKAATKKPFGVNVPLIYPQIEEIMDIIVEEQVPIVFTSAGSPKKWT
                     HFLKDKGIKVVHVVSSTVFAVKSQEAGVDAVVAEGFEAGGHNGREETTTFCLIPNVRR
                     AIDIPLIAAGGIATRQSYEAAFVLGAEGVQIGTRFALCTESSAHPLFKERCRAIGEGG
                     TKLLLKKIVPTRLAENDFSKAVEEAENKGASKEELLRLLGKGRAKKGIFEGDLQEGEL
                     EIGQIASMVTTEESAADIIRELTQ"
     gene            2070..3434
                     /locus_tag="P5293_07440"
     CDS             2070..3434
                     /locus_tag="P5293_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRR49766.1"
                     /translation="MTNSKRDGFSNRFGVLVAVAGSAVGLGNLWRFPYLVGNNGGAAF
                     IVVYIIMAIFLCLPIMISEFVIGRRTQSNAIGAIKKLAPHSGWILIGIISVATAFMIS
                     CFYSVVGGWTLEYLFHSLSKGFLINDNVALTSSFKNFTASPVRPAAMMLLYLGINAFI
                     VSAGIKKGIERYSKILMPILFILIIILAIRSITLPGASQGLNFFLNPDFSKFKAGSIL
                     AALGQAFFSLSVGMGCIITYGSYVNKKENLLNISLTTVIADITFAFLAGLAIIPAVFA
                     FGISPSEGPGLVFITLPQIFAQMPFGQLISFVFFTILLFAAITSSISLLEVIVAYLVE
                     ELNMRRKNATLLTFIVIGFFGILCSLSQGTLKDFTLFGKNLFDLFDYTTSNIMLPLGG
                     MLIVLFVGWKMKKSDVMDELFNGGTIQFKENLFKWIISGIKFLAPVAIAFILLNGLGL
                     IKLL"
     gene            complement(3508..3984)
                     /locus_tag="P5293_07445"
     CDS             complement(3508..3984)
                     /locus_tag="P5293_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49767.1"
                     /translation="MSTNISHPETVKILDKKAVIITSVIALSGIVSLILSWFLLASIL
                     FILAVIFFLSKSKITVYKPSGSRIKRESLFFDKEQLTILKSILEGGVDEGHLVKFDQT
                     GSAKVDVIYSADKQFAAFQLFEFVPHKYEEYSKLITQTGDLAKRSVEYIERCKKAN"
BASE COUNT         1222 a          817 c          701 g         1265 t
ORIGIN      
        1 tcaatagagc aataagaatg gtagagcgac gaaatccata cgtgaatttc aaagtgggct
       61 ttctttggga aataatgaca gcaatccagg aaaagaccaa agccaaaaca tcactaaagt
      121 tatggcctgc atcagctatc agcgccattg aatttgacaa taatccgtaa attatctcaa
      181 cagctataaa tattacgttg agaataatgc cccatttaaa ggcacggcca ggattatcat
      241 gaatttgtat atggtcatgt tccatgaaat aatcattttt aatctcattt ttttcaatat
      301 ataaaacaaa attgggtact cttttgttat gtatataaaa tcaattttaa tgaaacattt
      361 atttacaatt ctatttttta ttgctacagt tccatgttta gctgtagcac aaaataacaa
      421 caaattgtct aacaataaaa acaatatcaa tatgaaaaca ataagcacat tagaatttcc
      481 agaacttcca tattcaacag acgctcttga accttttatt gacaaacaga ctgttgaaat
      541 tcattatggt aaacaccaga aaacctattt tgacaatttc atggcagcag ttaaagggac
      601 agatgccgaa aacctcacaa ttctcgaact cttcaagaat atcagcaagt accctgctgc
      661 cgtaaaaaac aatggtggag gattttataa ccacgtacta tattgggaaa gcatcaaacc
      721 aaacggcgga aagccatctg caaagcttac cgatgccatt aacaagagtt tcggttcgct
      781 tgacgaatta aaaaaacaat ttaccgatgc cggtaaaaat cgttttggaa gtggttgggc
      841 atggctgtgt gttgacgaaa agggcaactt gtttgtcttt tcaactgcaa atcaagagag
      901 tcctttaatg gatgtcgctg aaaagcatgg aactcctctt attgcaatgg atgtatggga
      961 acatgcctac tatctcaaat atcaaaacaa aagagcagac tatatagagg ctttctggca
     1021 gataatcaat tgggaagttg tttctcaaaa gtacgaagca ctggttaaat aaccggattg
     1081 cccgttagct cattgagtga gctccctgat aatgtctgcc gcgctttctt cagtagtaac
     1141 cattgacgca atttgaccta tctcgagctc gccttcttga aggtcaccct cgaaaatacc
     1201 ttttttggca cgacctttac caagcaatct caaaagttcc tctttagaag cgcccttgtt
     1261 ttcagcttct tcaacagctt tagaaaaatc gttctctgca agccttgtag gaacgatttt
     1321 ttttagaagc aattttgtcc ctccctctcc aatagccctg cacctctctt taaaaagagg
     1381 gtgagccgaa ctctctgtac aaagtgcaaa ccgagtacct atctgcaccc cttctgcgcc
     1441 cagaacaaag gcagcctcat aactttgacg agttgcaatc cctcctgcag caataagcgg
     1501 aatatctata gcccgtctga catttggtat aagacaaaat gtggtggtct cttctctacc
     1561 attatgaccc cccgcttcaa aaccttcagc aactacagca tctaccccag cctcctggct
     1621 tttaactgca aaaacagtac tggagacaac gtgtactact ttaatccctt tatcttttaa
     1681 aaaatgtgtc cattttttag ggctgccggc agaagtaaaa acaattggta cttgttcttc
     1741 aacaatgata tccataatct cctcaatttg aggatagata agaggcacat ttactccaaa
     1801 aggctttttt gtggctgcct tgcaactctt gatatgatat ctgagattgt caggatgcat
     1861 cgatccggca cctataagac caagagctcc agcgttactt acagctgctg caagtcgcca
     1921 gccactgcac cagatcattc ccccttgaac aatcggaaac tctgtttcaa aaagtttaca
     1981 aactctgtta atcatagccc attagcaatt aagatccaaa tataagtaaa accaaacgtt
     2041 tttttatctt tgcttcaaac ttaaagctta tgacaaactc aaaaagagac ggttttagca
     2101 accgttttgg cgtattggtt gcggttgcag gttcagctgt cggactggga aatctttgga
     2161 gattccctta tctcgtaggt aataatggtg gtgctgcttt tatagtagtt tacatcatta
     2221 tggccatatt tctgtgcctt ccaattatga tttctgaatt tgtaattgga agaagaactc
     2281 aatcgaatgc aataggagca ataaaaaaac ttgccccaca ctccggatgg attttaatag
     2341 gaattataag tgttgccaca gcttttatga tatcctgctt ctattcagtt gtcggaggat
     2401 ggactttaga gtatctgttc cattcattgt caaaaggctt tttgataaat gacaacgttg
     2461 ctcttacatc ttcattcaaa aactttacag cttctccggt aagaccagct gccatgatgc
     2521 ttctgtacct tggaataaat gcatttattg tatctgcagg aataaaaaag gggattgaac
     2581 gttattccaa aatccttatg ccgatacttt ttatattaat aataattctc gcaattagat
     2641 caattacttt gcctggagca agtcaaggtt tgaatttctt tctcaatccc gacttttcaa
     2701 aattcaaagc aggcagtatc cttgccgctc tcggccaagc ctttttctct ctaagtgttg
     2761 ggatgggatg tatcatcaca tatggttcat acgtcaacaa aaaagaaaac ttacttaata
     2821 tctcgttgac cactgttatc gcagatatca cttttgcttt tcttgccgga cttgcaataa
     2881 ttccggcggt atttgcattt ggtatctctc cgagcgaagg acccggattg gttttcataa
     2941 cacttccaca aatttttgct caaatgccgt tcgggcaatt gatctctttt gttttcttta
     3001 caatacttct atttgctgct ataacttcct caatatcctt gctcgaagtt attgttgcct
     3061 atctggttga agaacttaac atgagaagaa aaaatgccac tttgctcacc tttattgtaa
     3121 tcggcttttt tggcattctc tgttcacttt ctcaggggac actgaaagat ttcacattgt
     3181 ttggtaaaaa tctctttgac ttgttcgatt atactacatc taacattatg cttccacttg
     3241 gaggaatgtt aatagttcta tttgtcggct ggaaaatgaa aaaatcagat gttatggatg
     3301 aacttttcaa tggtggtaca attcaattca aagaaaacct ttttaaatgg ataatttcag
     3361 gtattaaatt ccttgctcct gtagccattg cttttatttt actcaacggg ttgggattga
     3421 tcaagttact ataacaaaat tgtaagaaca aaaagaggct gcttactttt taggcagcca
     3481 ctttctgttc tatatgtcaa gttgtgatca attagccttc ttgcaccgct ctatgtattc
     3541 aaccgacctt ttagccaagt ctcctgtctg agttatcagt tttgagtact cctcgtattt
     3601 gtggggaaca aattcaaaaa gctgaaatgc tgcaaactgc ttgtcggcag aataaattac
     3661 atcaactttg gcgctgccag tttggtcaaa tttaaccaga tgaccctcat ctacacctcc
     3721 ttccaaaata gattttaaaa ttgtaagttg ttccttatca aagaacaaag actctctctt
     3781 tatacgactt ccggacggtt tgtaaactgt aatttttgat ttggacagaa aaaaaataac
     3841 tgcaagaatg aacagaatgc ttgcaagcag aaaccatgaa agtatcagtg aaacaatacc
     3901 tgaaagagca atgacagatg taataataac tgccttttta tctaatatct tgactgtttc
     3961 agggtgtgat atatttgtgc tcatatttta ccttttattt aaata
//
LOCUS       DAOTQL010000138         3922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_17914_length_3922_cov_6.436773, whole genome shotgun sequence.
ACCESSION   DAOTQL010000138 DAOTQL010000000
VERSION     DAOTQL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17914_length_3922_cov_6.436773"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="P5293_07450"
     CDS             complement(<1..852)
                     /locus_tag="P5293_07450"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR49768.1"
                     /translation="MQVTVKFKILPTKEQEQYLEETLDEYISTVNNIVQSMINNRHIK
                     LTSKDIEANLPSAVKNQAIQDAKSVYKKYKKTKKLSILKKPVCIWNNQNYKINDCLSF
                     PVLINGKSQRIKVKMLLADYQKQQLNNKLGSLRINKKSGKWIVQIAVEIAEKQNNNQN
                     VMGVDLGLKVPAVATTKNGKTKFLGNGRQNKYIRRKYKALRQKLGKAKKLKKIKQISD
                     KEQRWMKDQDHKISKQIVNFAIENNVSVIRMENLTNIRNTARTSRKNKKNLHTWSFYR
                     LAKYIEYK"
     gene            complement(941..3145)
                     /locus_tag="P5293_07455"
     CDS             complement(941..3145)
                     /locus_tag="P5293_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49769.1"
                     /translation="MKLEIKNVKFRNFFSYGKVLQDVDFQPGINLVLGNDVTKDRSNA
                     VGKSGFLETIPFALFGKVNRNVRKESIVNWKNRKNCEVYLTFTKGGMEYTIFRALKPD
                     KLEVYKNGNLMPTPSHKIDYQKEIEDEIIGMDFKTFISLVYSNLNSSVPILSMTADKK
                     RKFIETVFGLELFSQLSVKCNEKLKVVADKIFTYKTRKEYIEKNIEDIQRQVESIRQQ
                     VSGIMSHEDELETVKKEYKETKNGSLIKKLESFKEKKRKKQEEFDGLSKSMYDLVSKT
                     SIAESQIKFIDSQLERIGDVDKDYENFESAMKEYGLYLKEHISVEELDARISSMMEDV
                     NQINHNIDSIRKDLDSVTHDIIESKTTLKSSTSGLELLLGVTVCPTCGSVIDSDKLAK
                     QKVKSTKELNKRIDGLEKKKETLTTMMSEAKENRIKLNGEIKNFTDIKIKMIELQGKI
                     ASYKHLDEKRENSLSLQKEKKEKIKEIECLKKEQTKLSKKMENLEDEIKELNKELIEL
                     NERNDKIHALKSKIIILEEKVKHERESKERFQVLIKEHEEKIEELQREKRSMSAQMTK
                     LAGVSDYLDFVKMICRDDHVKQYAISSIVPFLNKRVNHYLSEVGHSFYIILDNWLDEE
                     IKGPGIINCSYGNLSGGEAKSVDFALQMALLDIARIQAGIFPDILELDEILDSSLDSY
                     SIEKVMQILKIKQQEDGSKIFLVSHRKEVNDVDIDALYLVEKKNGFSYIAKV"
     gene            complement(3149..3448)
                     /locus_tag="P5293_07460"
     CDS             complement(3149..3448)
                     /locus_tag="P5293_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49770.1"
                     /translation="MTDYEKIGQAVGKIVTKKQLAYGDSFGQSGKVMKILYPDGIKIE
                     QMDDALCVVRILDKLFRLATSPNALGESPYQDIAGYALLGLARQIKEKKEPAKEE"
     gene            complement(3450..3884)
                     /locus_tag="P5293_07465"
     CDS             complement(3450..3884)
                     /locus_tag="P5293_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49771.1"
                     /translation="MDRDDVMNTISISSTSISDSISIIRDYKINWTGGSTSTCSCTST
                     GWNYAINFPSIRDVLILVRNYVNRADTVDDFFQLSERINFICEILEADIKLTSEQIMS
                     IFSLSYKEFQFLNKRKKEYRKILEKWSNKKKDIVLEDKLFEV"
BASE COUNT         1032 a          770 c          513 g         1607 t
ORIGIN      
        1 tttatattct atatatttag caagacgata aaaagaccat gtatgcaaat tctttttgtt
       61 tttacggctt gttcttgccg tgtttctgat attcgttaaa ttctccatac gaattacaga
      121 aacattattt tctattgcaa agtttactat ttgcttgctt atcttatgat cttggtcttt
      181 catccatcgt tgctctttat cacttatttg tttaatcttt tttagttttt tagctttgcc
      241 taatttttga cgcaatgctt tgtatttacg cctaatatac ttattctgcc taccattccc
      301 caaaaatttt gttttaccat ttttagttgt tgctacagca ggtactttta aacctaaatc
      361 cactcccatt acattttggt tgttattttg tttttcagct atttcaacag ctatttgtac
      421 aatccatttg cctgattttt tatttattct taatgagcct aatttgttat ttaattgctg
      481 tttttgatag tctgctaata acatctttac tttaattctt tgtgattttc cattaattaa
      541 cacaggaaaa cttaaacagt catttatttt ataattctga ttattccata tacatactgg
      601 tttctttagt attgataatt tcttggtctt tttatatttt ttataaacac ttttagcatc
      661 ttgtatagct tgatttttta ctgcacttgg tagatttgct tcaatatctt ttgacgttag
      721 ttttatatgt ctattattaa tcatactttg aacaatatta ttaactgtag atatatattc
      781 atctaaagtt tcttctaaat attgttcttg ttctttagtc gggagtattt taaattttac
      841 tgttacttgc ataactcacc gcttattctc acattcaagg atttaattgt ttgcccaata
      901 taagatttat tattgataat attagttgct ttataaatta tcatactttt gctatataac
      961 tgaatccgtt tttcttctcc actaaatata aggcatctat gtcaacatcg tttacttctt
     1021 tcctgtggct aacaaggaat atcttgcttc catcctcttg ttgtttgatt tttagtattt
     1081 gcataacttt ttcaattgaa tagctgtcaa gcgagctgtc cagtatctcg tccagctcaa
     1141 gaatatcggg aaatatacct gcttgtattc tggctatatc caatagtgcc atctgtaagg
     1201 caaaatcaac actcttcgcc tctccaccag ataaattacc gtaggaacag ttgatgatgc
     1261 cggggccttt aatttcttcg tctaaccagt tgtcaagaat aatatagaag gaatgaccaa
     1321 cctctgataa atagtggttc actctcttat tcaggaatgg aacgatagaa ctgatagcat
     1381 actgcttaac atgatcatca cgacaaatca tctttacgaa atcgagatag tcacttacac
     1441 cagccaactt tgtcatctgt gctgacattg accgtttttc tctctgtaac tcttctatct
     1501 tttcttcatg ttcttttata agaacttgaa atctttcttt cgattctctt tcatgtttta
     1561 ccttttcttc cagtatgatt atcttactct taagagcatg aatcttgtca tttctttcat
     1621 tcagttcaat caattcttta ttcaattctt ttatctcatc ttccagattt tccattttct
     1681 ttgaaagctt tgtttgttct tttttcagac attctatttc ttttattttt tctttcttct
     1741 ctttctgtaa tgataaacta ttttctctct tttcatcaag atgtttataa gaggcgattt
     1801 tcccctgtag ttctatcatt ttgattttta tatctgtaaa attctttatc tcaccgttga
     1861 gttttatcct attctcttta gcttccgaca tcattgttgt aagagtttct tttttctttt
     1921 ccagtccatc tattctctta ttcaattcct ttgtgctctt tactttctgt tttgctaatt
     1981 tgtcactatc aataacagag ccacaagtgg ggcatactgt tacccccaat aacaattcaa
     2041 gcccactggt tgatgatttg agcgtagtct tactttctat aatgtcatgg gttacagaat
     2101 ctaaatcctt tctgatggag tcgatattgt gattgatttg atttacatcc tccatcattg
     2161 aagaaattct tgcatcgagt tcttctacgg aaatgtgttc cttgagatag agaccatatt
     2221 ctttcattgc actctcaaag ttttcataat ccttgtcaac atcccctatt ctttccaatt
     2281 gagaatctat aaattttatc tgactttctg ctatggatgt ttttgatacc aaatcataca
     2341 tactttttga cagaccatcg aattcttcct gtttctttct tttcttttcc ttgaagcttt
     2401 ccaatttttt aatcaaactt ccattcttag tttccttata ttccttcttt actgtttcca
     2461 gttcatcttc atgggacata atacctgaaa cctgttgtct aatactttca acttgtcttt
     2521 gaatatcttc gatgttcttt tctatgtatt cttttcttgt tttataggta aatatcttat
     2581 ctgcaactac tttcaacttt tcattacact taacactcaa ttggctaaac aattccaaac
     2641 caaaaactgt ttcgataaat tttcttttct tgtctgctgt cattgataat attggaacgg
     2701 atgaattgag attggaataa acaagagaga taaaggtctt gaaatccata ccgatgattt
     2761 catcttctat ctctttctga taatctatct tatgtgaagg tgtgggcatc agattgccgt
     2821 ttttgtaaac ttccaactta tccggtttta gtgcacggaa aatggtatac tccattccac
     2881 cttttgtgaa ggtaagatag acttcacaat tttttctatt cttccagttt acaatgcttt
     2941 cctttctgac atttctgttt actttgccaa aaagggcaaa aggaattgtc tcaagaaaac
     3001 cggacttacc gacggcatta cttctatctt tagtcacatc gtttcctaag acaagattta
     3061 tgcccggttg gaagtcaaca tcctgtaaca ctttcccata actgaaaaaa ttacgaaact
     3121 ttacattctt gatttcaagt ttcatagatt attcttcttt agccggttct ttcttttcct
     3181 ttatttgtcg tgcgaggccc aatagtgcat acccagcaat gtcctgatac ggactttctc
     3241 caagggcatt cggtgatgtt gccaacctga aaagcttgtc aagtatcctg accacacaga
     3301 gagcatcatc catctgttca attttaattc catctggata taggattttc atgacttttc
     3361 cggattgccc gaagctgtca ccataagcca attgcttctt agtgactatc tttcctacag
     3421 cttgtcctat tttttcataa tctgtcatat tatacctcaa acaatttatc ttccaataca
     3481 atatcttttt tcttattaga ccacttctct aaaatctttc tatactcttt ttttctttta
     3541 ttcaagaatt ggaactcttt gtatgataga gaaaaaatac tcatgatttg ttctgatgta
     3601 agtttaatat cggcttccaa tatttcacaa ataaaattta ttctctcact aagctggaaa
     3661 aaatcatcaa ctgtatctgc cctgtttaca tagttacgaa ccagaatcaa aacatctctt
     3721 atgctcggaa aatttatggc gtagttccaa ccggtgctcg tgcacgagca cgtgctcgtg
     3781 gagcctccgg tccaattgat tttataatct ctaataatag atatactatc cgatattgat
     3841 gtgcttgata tactgatagt attcataaca tcatctctat ccataaaaat aacctcctga
     3901 taagattttc aattattata cc
//
LOCUS       DAOTQL010000139         3891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_18093_length_3891_cov_4.012774, whole genome shotgun sequence.
ACCESSION   DAOTQL010000139 DAOTQL010000000
VERSION     DAOTQL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18093_length_3891_cov_4.012774"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="P5293_07470"
     CDS             complement(<1..1815)
                     /locus_tag="P5293_07470"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HRR49772.1"
                     /translation="MSDNLIKVRGARVNNLKDISVDIPRDKFIVVSGISGSGKSSFAF
                     DTVYAEGQRRFVESLSSFARQFLGRMSKPDVDDISGIPPAIAIEQKVNTSNSRSTVGT
                     MTEIYDYLRLLYARIGRVFSPISGREVKRDSLEDIVEYVLGFSEGTTLYIIAPLGVKD
                     DEGFTERLLELKNLGFTRLFKEGEGDVIRIENILTGKSTISEKTYLLVDRVIVENSEE
                     FKGTLMDSLQVAMGVNASTSGQRGAVLISTGKTEERPREFSTLYEADGISFEEPTELL
                     FSFNNPLGACPFCSGLGRVPGIDESLVIPDKTLSVYQGAVACWKGEMMSYYLNELINN
                     AWKFDFPIHKPYLELSEEQKELLWNGNEYFTGINDFFKIVDANSYKIQYRYMKSKYTG
                     WSVCPKCHGKRLRKEALYVKIGGKSIDELLEMSIDNLYKFVQNLTFDNYELAVISRVL
                     KEIKSRLECILSVGLSYLTLNRASRTLSGGESQRINLVSSIGSSLVGSLYILDEPSIG
                     LHPRDTDKLIDVLKQLRDTGNTLIVVEHDEEIIRSADHLIDFGPLAGINGGEVVYQGL
                     PVKKISLEECGKSKTLAYLSGFEKIETPLKRREWRHYIE"
     gene            complement(1808..>3891)
                     /locus_tag="P5293_07475"
     CDS             complement(1808..>3891)
                     /locus_tag="P5293_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRR49773.1"
                     /translation="RSHDIASEQLSGLSRGNGLPFMDHVVAVAEIVAFEIGLSPEAIA
                     AVFMHEASRSNNELLDSCKKEFSDEIIAMARSLNKISEIKPKDTGLLSDNYRKLIVSY
                     SHDPRVSLIKLADRLEVMRNLDFFVKSKQIKKATETLLLYAPLAHQLGLYNFKSEMED
                     LSFKYTDPEHYRIITNKLKAGEQDRKRLMDIFVKPIEMSLRKAHIPYKLKSRTKTAYS
                     IWKKMQAKQIPFEEVADIFAIRIVIDVPDASPEKEKELCWKVYSFVTEHYEPDTSRLR
                     DWITVPKSNGYESLHTTVTAQNNQRIEVQIRTSRMDDIAENGYASHWLYKGVKNDTSL
                     NAWLKSVKSLLESPYKKPEAQFNIFTNDVFVFTPEGDLRKLPADSSVLDFAFDIHTNL
                     GLKCSGAKINGKLSSIREKLKTGDVVEVITSKNQRPSSDWLNFVVSSKAKAKIRQKLN
                     EELGKQIQAGKELFERRMSNWKIDVNDEVLNFLVKQCKFKSIGEFYSALANGTFDIMD
                     VKNILSSREEIEERGVGEESSLQSPKNIQKSCDGSDILIIDDKLNNVGYKLARCCNPI
                     MGDEVFGFVTVKDGIKIHRMGCPNAARLISNYPYRVQKVKWKESSEGSNFQTSIKVSS
                     YLDFGLSQQIMEIVNNLSISMRFFSISENKGMIEAKMQVAVPNNQVLDKLLFNLRKIK
                     GVKSVTRITNV"
BASE COUNT         1162 a          849 c          569 g         1311 t
ORIGIN      
        1 ctcaatataa tgacgccatt ctctacgttt taaaggcgtc tctatctttt caaatcctga
       61 caaataagca agagttttac tctttccaca ctcttcaaga gagatctttt taacaggtag
      121 tccttgataa actacctctc cgccatttat acctgcaagt ggaccaaaat caataaggtg
      181 atccgcactt cttattatct cctcatcatg ctcaaccaca atcaacgtat taccggtatc
      241 tctaagctgt ttaagtacat ctatcaattt atctgtatct cttggatgaa gtccaatgct
      301 tggttcatca agaatgtata atgagccaac taaactgctt cctatagaac tgacgagatt
      361 tatcctttga ctttcacctc cgcttaatgt tcttgatgcc ctgttaagtg tcaaataaga
      421 taaaccaaca gataatatac actcaagacg actcttaatt tccttcagaa cacgagaaat
      481 tactgctaat tcgtaattgt caaatgttaa attttgcaca aatttataga gattgtctat
      541 gctcatttca agcagttcgt caatactttt cccaccaatt ttaacatata atgcttcctt
      601 tctcagtctt tttccatgac actttggaca gacactccag cctgtatatt ttgatttcat
      661 gtaacgatac tggattttgt aggaatttgc atccacaatt ttgaaaaaat cgtttatacc
      721 ggtaaaatat tcattgccat tccacagtaa ttctttttgt tcttcagaaa gctccaggta
      781 aggtttatga attggaaaat caaatttcca tgcattattt atcagttcat ttaaataata
      841 actcatcatt tctcctttcc aacatgcaac agcaccctga taaacagaaa gtgtcttatc
      901 cggaataacc agcgattcat caattccggg aactctgcca aggccggaac aaaatggaca
      961 agctcccaac ggattgttaa aactaaaaag taattcagtg ggttcctcaa agcttatacc
     1021 gtcagcttca tatagtgttg aaaattctct aggtctctct tctgttttcc ctgtagagat
     1081 caaaactgct cccctctgac cagaggttga agcattcact cccattgcta cctgtaatga
     1141 atccatcaat gtacccttga actcttcact gttttccact atgactctgt caacgagtaa
     1201 atatgttttc tcagaaatag tagatttacc tgtaagaatg ttttcaattc taataacatc
     1261 accctctcct tctttaaaaa gtctggtaaa tccaagattc ttcaactcaa gcaaacgctc
     1321 agtaaaacct tcatcatcct taacacctaa tggtgcaatt atgtacaaag tagtcccctc
     1381 agaaaatcca agtacatatt ccacaatgtc ttcaagcgaa tctcttttga cttcgcgacc
     1441 actaattgga gaaaataccc tacctatcct tgcatacaac aatctcaaat aatcatatat
     1501 ctcagtcatt gtaccaactg tagaacgtga attggacgtg ttaaccttct gttctatcgc
     1561 aatagcagga ggtattccag aaatatcatc cacatcaggc tttgacattc tgccaagaaa
     1621 ctgtcttgca aaagaagaga ggctttcaac gaatcttctt tgtccttctg cataaacagt
     1681 atcgaatgca aaactgcttt tacctgatcc tgaaattccc gatacgacaa taaacttatc
     1741 tcgtggaata tcaactgata tatccttaag attgttaacc ctcgctcctc ttacctttat
     1801 taaattgtca gacatttgta atcctggtta ccgatttaac acctttaatt tttctgagat
     1861 taaacaataa cttatccaat acttgattgt ttggaactgc gacttgcatt ttagcttcaa
     1921 tcatcccttt attttctgaa attgaaaaaa atctcataga aatagataag ttgttaacaa
     1981 tctccataat ctgttgacta agcccaaaat ctaaatatga actcactttg attgaagttt
     2041 ggaaattact gccttctgat gattccttcc atttgacttt ctgtaccctg tatggatagt
     2101 tgcttataag tctcgccgca tttggacatc ccatcctgtg gattttaatt ccatccttaa
     2161 cagttacaaa gccaaacact tcatcaccca ttatagggtt acaacacctg gccaatttat
     2221 atcccacatt gttaagtttg tcgtcaatta tcaaaatgtc agaaccgtca caactctttt
     2281 ggatattttt tgggctttgt aatgatgact cttctcctac tccacgttct tctatttctt
     2341 cccttgaaga gagaatgttt ttgacatcca ttatgtcgaa tgttccattt gcaagtgctg
     2401 aataaaattc cccaatgctt ttgaacttac actgcttgac caaaaaattc aaaacttcat
     2461 cattgacatc tattttccag ttggacattc tacgttcaaa cagttctttc cctgcctgaa
     2521 tctgtttccc aagttcctca ttaagtttct gtcttatttt tgcctttgcc ttggaagaaa
     2581 caacaaaatt gagccaatca ctggaaggtc tctgattttt actggtaatt acctccacca
     2641 catcgcctgt tttaagtttt tctcgtatag agctaagttt accattaatc ttagccccgg
     2701 aacatttcag tccaagatta gtatgaatat caaaagcaaa atccaataca cttgaatcgg
     2761 ccggtaattt tctaagatct ccttcaggtg taaatacgaa tacgtcgtta gtaaaaatat
     2821 taaattgcgc ctctggcttc ttgtatggtg attcaagaag actttttaca cttttaagcc
     2881 aggcattgag acttgtatca ttcttaactc ccttgtacaa ccaatgtgaa gcataaccat
     2941 tctctgctat atcatccatt cttgaggttc ttatctgaac ctctattcgt tgattatttt
     3001 gagcagttac agtagtatga agtgattcgt agccatttga ttttgggaca gtgatccagt
     3061 ctctaagtct tgaagtatca ggctcgtaat gttctgtaac aaaagaatat accttccaac
     3121 aaagctcttt ttctttctca ggagaggcat caggtacatc tattactatc ctaattgcaa
     3181 aaatatcagc tacctcttca aaagggatct gcttagcctg catctttttc caaatagaat
     3241 atgctgtttt agtcctgctt tttagcttgt agggtatatg agctttccta aggctcatct
     3301 ctataggttt tacaaaaata tccatcaatc ttttgcgatc ctgttctcct gcctttaatt
     3361 tattagtaat tatacgataa tgttcaggat cagtatactt aaaagaaaga tcttccatct
     3421 cgctcttaaa attgtaaagc cctaactggt gtgcaagagg tgcgtataag agcagagtct
     3481 ctgtcgcctt ttttatctgt ttggatttga caaaaaagtc caaatttctc atgacttcaa
     3541 gacggtctgc aagtttaatc aaactaactc ttgggtcatg tgaatatgaa actatcaatt
     3601 ttctgtaatt atcagaaaga agtccagtat cttttggttt aatttcagaa atcttattta
     3661 agcttctggc cattgcaatt atttcgtcag aaaactcttt tttgcagcta tcaagtaatt
     3721 cattatttga ccttgacgcc tcatgcataa atactgcagc aattgcctcc ggagaaagtc
     3781 caatttcaaa agccacaatt tcagcaacag caacaacatg atccataaat ggcaagccat
     3841 tacctctact caagccagaa agttgctctg atgcaatgtc atgagatctt a
//
LOCUS       DAOTQL010000140         3875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_18183_length_3875_cov_4.975916, whole genome shotgun sequence.
ACCESSION   DAOTQL010000140 DAOTQL010000000
VERSION     DAOTQL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18183_length_3875_cov_4.975916"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1986
                     /locus_tag="P5293_07480"
     CDS             <1..1986
                     /locus_tag="P5293_07480"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HRR49774.1"
                     /translation="KEEPTGILLFILHRVKQVALIICYFCILIKNNDMNHCARLLAFV
                     LVITIISSCEKDITPVIELTQTTINLPTNESSAEVSFTANTNWTATISGTWCTISPSN
                     GKGDATITISASDNFSSRARSAVLKIVSQNLVKEVTVSQDYAQLSTNVLELSFPKNAT
                     TQQLTITSNTKWIIELPLNLVWVTVGTLSGEGNATVNVSVNENTSGPARSSYITIKYG
                     NESKIITISQARSQNSLPSLPQLTSPSSNATNVATLTNFVWTASSDADGDNVFYTLSY
                     TKDNINWKDTTVTVTSVYLPSHLEENTRYSWRVTANDGIESVTTSTSSFTTGSKRGLA
                     EREYSIALDAIEPGSGGKAADIIFVGDGYTAADFQEGGKFHIDVQEGINGLFNVEPYK
                     TYKSHFRVYKVGSYSNESGVTQLDKSIVKNTVFSSSFNGGSNITANFDKVFEYCKNIS
                     SITTDALKATLVIVLLNQDRYAGTTYIWTDGSVISLTPVAKSYPAGSQYVNILVHEAG
                     GHGFGGLADEYITNQGQTITSSAISDYNQWNALGFFANIDIVSDLTAVKWKHFIGLEG
                     YSRVGVYEGAFGFSYGVWRAESTSCMIDNILYFSAPSREAIVKRIMTRTGNIYSFESF
                     KANDVERAPSSAAAATYSKGFNNITFQPLSPPILLKK"
     gene            2026..2598
                     /locus_tag="P5293_07485"
     CDS             2026..2598
                     /locus_tag="P5293_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HRR49775.1"
                     /translation="MSNDIKYKLAQGNIEAFEYLFNKYYSPLCAYARRYLKDKDAAED
                     IVCSLFLYLWEHRERISLISNLESYLLVAVHNSSLNKLRENSNRQSREESVTSDDQIA
                     TSNIDDNISEKIDHFIEKEIISTVINDVYKSLPENCKKIYKLRREDNLTYREIAEQLN
                     ISIGAVKKQLSRANKKIREALKKYTFSLFI"
     gene            2683..3705
                     /locus_tag="P5293_07490"
     CDS             2683..3705
                     /locus_tag="P5293_07490"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRR49776.1"
                     /translation="MDINEAVALINLFLRGEISEVEKVTILELIERDKRIKKIYFSMS
                     KKWLESYCEVEEEAENRKKAWQDLQLLIARSVIRNRKKLIYRTAFASVMIASVIFVAI
                     FVLHIHNRNTSQINALLSNARTEIVVPYGSKVKLSLPDSSTVWVNAGSTLSYNNSFSK
                     SNRDVTLDGEAYFEVAKNRQLPFKVFANNVCVEAKGTSFNIESYRQNVTTTLITGTVL
                     ISSKYGGDQILRPNQSVCYNSINREFDKVKNVTNAELYSSWKDNIWVIRSMKMDEFIE
                     RLEKRYNVVIIENGCEFQNKRISASLSNETIEQVMRALKSTMGVEYSINKNVITLRQI
                     DADNIH"
BASE COUNT         1325 a          610 c          752 g         1188 t
ORIGIN      
        1 aaggaggagc cgaccggtat cctccttttt attttacata gagtcaaaca agttgcgtta
       61 attatttgtt atttttgtat attaattaag aataatgaca tgaaccattg tgcaagactt
      121 ttggcatttg tgctggttat tacaataata tcttcttgtg agaaagatat tactccggta
      181 attgaactga cccagacaac tataaacttg ccaacaaatg aaagtagtgc agaagtttct
      241 tttacagcaa atactaactg gactgctact atttccggta cctggtgtac aatttctcct
      301 tcaaatggca aaggtgatgc tacgattact atctcagctt ctgataattt ctcttctcgg
      361 gcaaggagtg ctgttcttaa gatagttagc cagaatctcg tcaaggaagt aacagtcagt
      421 caggactatg ctcaacttag caccaacgtt cttgagttga gttttccgaa gaatgcaacg
      481 actcagcaat tgacaattac ttcaaatacg aaatggataa ttgaattacc tcttaatctt
      541 gtatgggtga cagttggcac attgtccgga gaaggtaatg ctacggttaa tgtttctgtt
      601 aatgaaaata cttcggggcc tgccagaagt tcatacatta caataaaata tggcaatgaa
      661 tctaagatta ttacgatatc acaggcaagg tcacaaaatt ctttgccatc tttaccccag
      721 cttacttcac cttcatcaaa tgccacaaat gtagcaacac ttacaaactt tgtctggaca
      781 gcatcttcag atgctgacgg agacaatgtg ttctatactc tttcctatac aaaggataat
      841 attaattgga aggatacaac agttactgtc acgtctgtct acttgccatc tcatttagag
      901 gagaatacca gatattcttg gagggttacg gcaaatgacg gcatagaaag tgttacaaca
      961 tccacttctt catttactac aggatcaaag aggggattgg cagaaagaga atattctatt
     1021 gcgttggatg ccatagaacc aggaagcgga ggaaaagctg ctgacattat ctttgtagga
     1081 gatggttata cagctgcaga ttttcaggag ggaggaaagt ttcatataga tgtacaggaa
     1141 ggaattaatg gtctcttcaa tgttgagcct tataaaactt ataagtcgca tttcagagtt
     1201 tacaaagttg ggtcttattc taatgaaagt ggtgtaacac agttagataa aagtattgta
     1261 aaaaacactg tgttttcttc gagtttcaat gggggatcaa acataactgc caattttgac
     1321 aaggtatttg aatattgcaa gaatatttct tctattacta ctgatgcact caaggcgaca
     1381 ttggttattg tgcttttaaa tcaggaccga tatgccggga caacatacat ttggacagac
     1441 gggtctgtta tatcacttac tcctgtggcc aagagctatc ctgcaggcag tcaatatgta
     1501 aatatacttg ttcacgaagc aggtggacat ggttttggtg gcttggcaga tgaatatatt
     1561 actaatcagg gacagacaat tacttcgagt gcaatatctg actacaatca gtggaatgca
     1621 ttgggttttt ttgctaacat agatatcgta tcagatttaa ccgctgttaa gtggaagcat
     1681 tttattggct tggaaggtta ttcgagagta ggagtttatg aaggagcatt tggattttca
     1741 tacggggtgt ggagagcaga aagcacaagc tgtatgattg ataatattct atatttcagt
     1801 gcaccatccc gtgaagcaat tgtgaagagg ataatgactc gaacaggcaa catatactcg
     1861 tttgaatctt tcaaagcgaa tgatgttgaa agagctccat catccgcggc agcggccaca
     1921 tattctaaag gatttaacaa cataacattc cagccactga gtccgccaat ccttttaaaa
     1981 aagtaattta gatgtcattt gctattttga atggagatta ttcatgtgag taatgacatt
     2041 aaatataaac ttgctcaagg caatatagaa gcttttgaat atctgttcaa caagtattat
     2101 tcgcctcttt gtgcctatgc tcgtagatac cttaaagata aagatgctgc agaagacatt
     2161 gtttgctcac tttttctata tctttgggaa catagagaac gaatatcttt gatttcaaat
     2221 ctggaatcat atcttctagt ggcagttcat aattcttcct taaataaact cagagaaaat
     2281 tctaatagac aaagtcgtga ggaatctgtt acatctgatg accagattgc cacttcgaac
     2341 attgacgata atatatctga gaaaattgat cattttattg aaaaagagat tatatcaaca
     2401 gttattaatg atgtctacaa atcacttccc gaaaattgta agaaaatcta taaattaaga
     2461 agggaagata atcttactta cagggagata gctgagcaac tgaatattag tataggtgcc
     2521 gtaaaaaaac agctgtcaag agctaataaa aaaatcagag aagctctgaa aaaatataca
     2581 ttttctcttt ttatataatt tcttttttta agtattaaga aattttgtca acacaggtcg
     2641 ttttaatgtg tcttattaaa aacgacagta gaagttataa ttatggacat taatgaagca
     2701 gttgcattga taaatctctt tttaagagga gaaattagtg aagttgaaaa agttacaatt
     2761 ttagaattaa tagaaagaga taaaagaatc aaaaaaatat atttctcgat gtcaaaaaaa
     2821 tggctggaaa gttattgtga agttgaggaa gaagctgaga atagaaagaa agcctggcaa
     2881 gatttacaat tgctaattgc gagaagtgtc ataagaaata gaaagaaact aatttacaga
     2941 acagctttcg cctctgtaat gatagcttca gtcatatttg ttgcaatatt tgttttacat
     3001 atacataacc gcaatactag tcaaataaat gcattactga gtaatgcaag gaccgaaata
     3061 gtagttcctt atggttccaa ggtgaaatta tctttacctg atagttcaac agtgtgggtt
     3121 aatgcaggtt ccaccttgtc atacaataac agtttttcta aatcaaacag ggatgtaacg
     3181 ctggatgggg aggcatattt tgaagttgcc aaaaacagac agttaccatt taaggttttc
     3241 gccaacaatg tatgtgttga agctaaaggc acatcattta atattgaatc ttataggcaa
     3301 aatgttacaa ccactttgat aacaggtact gtgctcattt ccagcaaata tggaggagat
     3361 caaattctca gaccgaatca atcagtatgc tacaattcta taaacaggga gtttgataaa
     3421 gttaaaaatg taactaacgc agaattgtat tcgtcatgga aagacaatat ctgggtaatt
     3481 cgttccatga aaatggatga atttattgaa agacttgaaa agagatataa tgttgtaata
     3541 attgaaaatg ggtgtgaatt tcaaaataaa agaatttcag cttctcttag taatgaaacc
     3601 atcgagcagg taatgagagc attgaaatca accatgggtg tggaatattc cataaataaa
     3661 aatgttatca cattaagaca aattgatgca gacaatattc actaaactaa tagctatgat
     3721 acaaaaaact tcaattattc taataattct gacatttttt gtcagtagtc tgttggccgg
     3781 agtgtcaacg gataagggtt ttgttctaca ggatggaaaa ataactatat ctgttacaaa
     3841 caaacccatc aaggagatac ttgatatgat agaga
//
LOCUS       DAOTQL010000141         3787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_18754_length_3787_cov_4.047160, whole genome shotgun sequence.
ACCESSION   DAOTQL010000141 DAOTQL010000000
VERSION     DAOTQL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18754_length_3787_cov_4.047160"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="P5293_07495"
     CDS             complement(<1..994)
                     /locus_tag="P5293_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRR49777.1"
                     /translation="MDVFTPEDNALIDKEFEELRLASLKRCADQSEYEMVLKAFEFAR
                     SAHNGVRRRSGEPYIIHPICVAKIVVQEIGLGYKSIVSALIHDVIEDTEYNIEDIQRL
                     FGAKIASLVDGLTKIKSVFDSSTTGQAENFKRIILTLNDDVRVILIKLADRLHNMRTI
                     DSMPQLKKDKILSETMYIFIPLAHRLGLYSIKSEMEDIWLKHSFPNEYDEIQARIAKT
                     VAERGFAIDQFIEPIAESLHQDNYEFTITKRTKTPYSIWRKMIEKHITFDEVYDLFAV
                     RIVFEPKPNQPERVQCWHIYSLITENYLSKTDRIRDWVSTPKANGYEALHCTVLG"
     gene            complement(998..2413)
                     /gene="hydG"
                     /locus_tag="P5293_07500"
     CDS             complement(998..2413)
                     /gene="hydG"
                     /locus_tag="P5293_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRR49778.1"
                     /translation="MNNRILSKDAEDFIDDKLLHSLIENTKEDKGRLREIFAKSKAKQ
                     ALNLEEVASLLAIKSRDGLEELYETARDLKRAIYGNRIVLFAPLYIGNNCINNCSYCG
                     FRSSLTNAVRKTLTKDELIDEVASLEREGHKRLILVYGEHPKYSPEFIADTVRTCYSV
                     KVDNGEIRRVNINAAPMNVEGYKIVKAAGIGTFQVFQETYHKETYAKYHPANTVKGDY
                     MWRLDAMDRAFEGGIDDMGIGALFGLYDWRYDVLGLVSHAIHLQKKLGVGPHTISFPR
                     IKPALGLNIDLPYEVTDEQFKQLVATLRISVPYTGLIMTARESPKIRDEVIEFGVSQI
                     DAGTRLEIGGYQEKRDRGQDLDREQFEVGDQRELDDAIRWLIERGFIPSFCTSCYRLG
                     RTGEHFMEYAIPGFIGNFCTPNAMLTLTEYLEDYSSEETKKAGYEMLENELAKIKNSE
                     RRAEIAGKIEQIKMGKRDLRY"
     gene            complement(2410..3339)
                     /locus_tag="P5293_07505"
     CDS             complement(2410..3339)
                     /locus_tag="P5293_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRR49779.1"
                     /translation="MKKLAVKHTYQRVLKFLMIDIWHLDLSELSVMKARIIKYLKVFI
                     MTVRGFTTDKANIQAHSLTYYSTMSLVPLVAVVFAVTKGFGYGNEMETLIYKYFEGNE
                     QIINYLLKFADNIIVSLENGLFGFITFIILISAVIYLMLNIEMAFNDVWKVSGGRPLH
                     KRLIFYPILLMSFPFILLIFMSFGLIYTDALKSIGLELDRYVPITSIFTWLLLYGVVV
                     LVFSMMYFLIPNTKVKFAAAFNSALILAFAFIVVQVLYVETQLLVTGLNTVYGVFAAI
                     PLFLVWMNISWTLILFGAELSHSFQNVDNYKIS"
     gene            complement(3366..>3787)
                     /locus_tag="P5293_07510"
     CDS             complement(3366..>3787)
                     /locus_tag="P5293_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRR49780.1"
                     /translation="AYASMYGNTEKMADYLGREMVERGIKNIYIYDVSKTHVSYILRD
                     IWKYNAVVLGSCAYNTGMHPMMAQLCHELETSSPKGKLYSLFGSYSWSGGGLKMLKEF
                     ASKMEWKLFADPVEIIGHPDSQKIKDLSTMADKLANY"
BASE COUNT         1174 a          775 c          585 g         1253 t
ORIGIN      
        1 tgccaaggac agtacaatgc aaagcttcgt atccatttgc tttaggtgta cttacccaat
       61 ctcttatcct gtctgttttg gaaagataat tttctgtaat gagagaataa atgtgccaac
      121 attgtactct ttctggctga tttggttttg gttcaaagac gattcttact gcaaagagat
      181 catatacctc atcgaaagtt atatgctttt ctatcatttt tctccatatg gagtaggggg
      241 ttttagtcct ttttgtgata gtaaattcat aattatcctg gtgtaaactt tctgctattg
      301 gctcaataaa ttggtcgatt gcaaagcctc tttctgctac tgttttcgca atacgagcct
      361 gtatctcgtc gtactcgttt gggaaagaat gttttaacca aatatcttcc atttcagact
      421 tgatactgta aagtccgagt ctgtgagcaa gaggaataaa aatgtacata gtctcactaa
      481 gaatcttgtc tttcttaagt tgaggcatcg aatctatagt cctcatgtta tgaagcctat
      541 ctgcaagctt aatcaatata actcgaacat catcatttaa tgttaggata attctcttga
      601 aattttcagc ttgtcctgtt gtagaactat caaatacgct tttgatttta gttaatccat
      661 caacaagaga tgcgatttta gctccgaaga gccgttgtat atcctctata ttatattctg
      721 tatcttcaat aacgtcatga attagagcac taacaatcga tttgtatcca agaccaatct
      781 cctgaacaac aattttagcc acacaaatag gatgtatgat ataaggttct ccagatcttc
      841 tcctcacgcc attatgtgca gaacgcgcaa attcaaaagc cttgagcacc atttcatatt
      901 cagattgatc agcgcatctt ttaaggctgg ccaatctcag ctcttcaaat tccttatcta
      961 taagagcatt gtcttcagga gtaaacacat ccatttgtca ataacgcaaa tctcttttac
     1021 ccattttaat ctgctctatc ttacccgcaa tctcagctct tctttcgctg ttttttattt
     1081 tagcaagttc attctcaagc atctcatatc cggctttttt tgtctcctct gatgaataat
     1141 cttcaagata ctcagtaaga gtaagcattg catttggtgt acagaagtta ccaatgaagc
     1201 ccggaatagc gtattccatg aaatgttcac cagttcttcc aagcctgtag catgaagtgc
     1261 aaaagctcgg gataaaccct ctctctatca gccaacggat tgcatcatcc aactctcttt
     1321 gatctccaac ttcaaattgt tccctgtcca agtcttgacc tctatctctc ttttcctggt
     1381 aacctccgat ttccaacctt gttccggcat caatttgtga aactccaaac tcaattactt
     1441 catctctaat ttttggactc tctcttgctg tcattatcaa tccagtatat ggcacagata
     1501 ttcttaatgt agcaacaagt tgtttaaatt gttcatctgt gacttcgtaa ggtaaatcaa
     1561 tattcaaacc gagtgcaggc tttatacgag gaaaacttat tgtatgagga ccaactccta
     1621 attttttttg caggtgaata gcgtggctta ctaatcctaa aacatcatat ctccagtcat
     1681 aaagtccgaa taaggcacct atgcccatat cgtcaattcc tccttcgaat gctctgtcca
     1741 ttgcatccag acgccacatg tagtcacctt ttacagtatt tgccggatga tatttagcat
     1801 atgtttcttt gtgatatgtt tcctgaaaaa cttgaaatgt tcctatacct gctgctttga
     1861 caattttata accctcaaca ttcattggtg ctgcattaat gttaactcta cgaatctctc
     1921 cattgtcaac ctttacactg taacatgtcc tgactgtatc ggcaataaac tcaggagaat
     1981 atttaggatg ttctccatag actaaaatca atcttttgtg tccctctctt tcaagagaag
     2041 ctacttcatc aatcaactca tctttagtaa gagtttttcg aacagcattt gtcagagaac
     2101 ttctaaaacc acaatatgaa caattgttaa tacagttatt accaatatat agtggagcaa
     2161 aaagcactat tctatttcca tatatagctc ttttcaaatc acgtgctgtt tcatagagct
     2221 cttccaagcc atcacgggat tttatagcaa gcaatgaagc tacttcttca agatttaaag
     2281 cttgtttagc tttacttttt gcaaaaattt ccctaaggcg acctttgtct tcctttgtgt
     2341 tctcaataag tgaatgcagt agtttgtcat ctataaaatc ttctgcatct ttagataaaa
     2401 ttctgttatt catgatattt tataattgtc aacattttga aatgaatgcg aaagttctgc
     2461 tccaaacaga ataagtgtcc aactgatatt catccataca aggaacagtg gaatagcagc
     2521 aaatacaccg tacactgtgt taagcccagt aacaagtaat tgggtttcaa catataaaac
     2581 ctgcacaacg ataaaagcaa atgcaagaat taaagctgaa ttaaatgctg ctgcaaattt
     2641 aactttagta tttggtatta aaaaatacat cattgaaaat actaatacaa caactccata
     2701 taaaagaagc catgtaaata ttgatgtaat tggcacatag cgatcaagct ccagacctat
     2761 tgatttcaaa gcatctgtat aaataagacc aaatgacata aatataagca gaatgaatgg
     2821 aaaagacatc aacaatatag ggtagaagat aagcctttta tgaagaggtc tacctccgga
     2881 aactttccac acgtcgttaa aagccatttc aatattgagc atcagatata taacggcaga
     2941 tattaaaatg ataaaagtta taaaaccaaa aagaccattt tcaagtgaaa caattatatt
     3001 atctgcaaat tttaacagat agttaattat ctgctcattt ccttcaaaat atttataaat
     3061 aagcgtctcc atctcattcc catatccaaa tccttttgtg acagcgaata cgactgcaac
     3121 caaaggtact agagacattg ttgaataata agtaagactg tgagcctgaa tattagcctt
     3181 atcggtagta aagcccctga cagtcataat gaacactttc aaatatttga ttatccgggc
     3241 tttcatcaca ctcagctcgg atagatcaag atgccaaata tcaatcatga gaaatttaag
     3301 cactctttga taagtgtgtt ttactgcaag ttttttcaag atattctact catgttttat
     3361 taatttcagt agttggccaa tttatcagcc attgtgctca gatcctttat cttttgactg
     3421 tcaggatgtc caattatctc tacagggtcg gcaaaaagtt tccattccat cttagaagca
     3481 aattctttaa gcatttttaa gccgcctcca ctccaactgt aagaaccaaa cagcgaataa
     3541 agtttccctt tgggtgatga agtttcaagt tcgtgacaga gttgagccat cataggatgc
     3601 attccagtgt tataagcaca tgaaccaaga actacagcat tgtatttcca gatatccctg
     3661 agtatgtatg aaacatgcgt tttggaaaca tcgtaaatat aaatattctt tatacctctc
     3721 tcaaccatct ctcgtccaag ataatcagcc attttttctg tatttccata cattgaggca
     3781 tatgcaa
//
LOCUS       DAOTQL010000142         3746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_19017_length_3746_cov_5.117312, whole genome shotgun sequence.
ACCESSION   DAOTQL010000142 DAOTQL010000000
VERSION     DAOTQL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19017_length_3746_cov_5.117312"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..313)
                     /locus_tag="P5293_07515"
     CDS             complement(59..313)
                     /locus_tag="P5293_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49781.1"
                     /translation="MDYINIIIKLYSNLDKELKITDYNPDKGIILQAKKGSRLVTIIK
                     KAGIPYNSKYAIFANGERFSKWKKLHQSCEISILKISGGG"
     gene            complement(442..1545)
                     /locus_tag="P5293_07520"
     CDS             complement(442..1545)
                     /locus_tag="P5293_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRR49782.1"
                     /translation="MIRHIAHNLILFSGLACFLTACGQLVVKKRKLENINLFILFALM
                     GIILLQFYSVVTFKILSYPVIISFHSTIMFIFSPILYFAYYVVAFPEIKPPKKFYLCF
                     IPAAIAFIGDLYLFFLDNHTKQTLVQNFFGNINSPGVTLFKLGASFAILQMLLYHLAL
                     IKVLIPALHNNNGRNIILITFIYSICSASSSIIAIPGYIQGNPDFIRYSAIIISISFI
                     FTYLIGIRYPDFLQLLSINVKKTSYSKSLLKGLNTGLLLQELELLMKDKKLFLNEEIT
                     LKDIAETLHISQHQLSQLINEKFNVNFNTFINSYRIEFAKSLLIEKPHLTVLTIAYES
                     GFNSKSTFYDAFTKHAGVTPLEYRKNHSLKKHS"
     gene            1711..2151
                     /locus_tag="P5293_07525"
     CDS             1711..2151
                     /locus_tag="P5293_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRR49783.1"
                     /translation="MKDFVKAFFKRTVLFSYLALVFTSFTFCGGGGDSAVEPSPENQW
                     TIMYYGDGDCDLEEDLLVDVEEMKEGFVDGQGINLIVLFDRHPAYSSDKSVFGEDFTD
                     TRLYRITANTVWRLNGGSEFPEISKNGSYEANMGSGETLKKYQY"
     gene            complement(2156..3268)
                     /locus_tag="P5293_07530"
     CDS             complement(2156..3268)
                     /locus_tag="P5293_07530"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HRR49784.1"
                     /translation="MIIDELILQMVKDVNKLSEEKDSNKYIKKHPIEKVFLLLLLQQY
                     SGIDNGRAFSLYSKKMIGDTTKTISQSELSKKLCYRLPVELFHDMFKLQLSRLQGKKS
                     KDIRKMKKLIRIIDSTALPATATMDYAKHRRNKNDFKLHTVIGEEYIPENVKLKNGNS
                     SDKKSLKWAVKEGFIHIFDRGYNDYSQFQWIEEKQAYFVTRALGNIKYNIMQNRKVGH
                     RQKEKGIIADRIIEVIADRKQNITYKMRMVTFRFIDSAGKEQTFSLLTNLLSYRSDEI
                     AELYRKRWNIEVVFRWIKMFLKIERWLSRSKNGVLIQIYTALCAYIMVLTAKMNDLKK
                     YRIMKDAIYDFIIKLKSSIRMFDRGSNIEELFLESS"
     gene            complement(3454..>3746)
                     /locus_tag="P5293_07535"
     CDS             complement(3454..>3746)
                     /locus_tag="P5293_07535"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HRR49785.1"
                     /translation="TNGKAERFVGLIRTEALRPNSPSYYGEGIKVIEKFVDEYNNNRY
                     HAGIDYLKPVDVFYGRGPAILATRREKLKQARQDRFTRNTELNRILMGDLPA"
BASE COUNT         1127 a          596 c          658 g         1365 t
ORIGIN      
        1 actggtaccg gtaatctcct gcatatattc tccttttaat gaatttgttt ttttattttt
       61 atccaccacc tgagatcttc agaatagaaa tctcacagga ttggtgaagt tttttccatt
      121 tagagaatct ttctccatta gcaaaaatgg catatttgct attatatgga atcccagcct
      181 ttttaattat agtgacaaga cggcttcctt tctttgcttg aagaataata cctttgtctg
      241 ggttgtaatc tgtaatttta agttctttat ctaaattgga ataaagtttt attataatat
      301 ttatataatc cataaatttt tgaaattcaa ataatattta gcctatgatt cttttttata
      361 tatcatattt cagataagat cgtccgaata tataatttcg gacttaaaat ttaagctaaa
      421 tggtattttt ttgccgtaat gttaggagtg tttttttaaa gagtgatttt tgcggtattc
      481 cagaggagta actcctgcat gctttgtaaa ggcgtcgtaa aatgtagatt tggaattaaa
      541 accagactca taagctattg taagcacagt taggtgaggt ttttctataa gaaggctttt
      601 tgcgaattca attctgtagg agtttataaa ggtattaaag ttaacattga atttttcgtt
      661 tataagttgc gaaagctgat gttgagatat gtgcagggtt tcggcaatat ctttaagggt
      721 gatctcttcg tttaaaaaga gttttttgtc tttcattaaa agctcaagct cttgcagtaa
      781 aaggcctgtg tttaatccct tgagcagtga ttttgagtag gaggttttct ttacattgat
      841 tgagagcagt tgcagaaagt ccgggtatct tatgcctatt aaatatgtaa atataaagga
      901 tatagatatg attattgcac tgtatcttat aaagtctgga tttccttgga tatatccagg
      961 tattgcaatt atagatgagg aggcagaaca aattgagtaa ataaaggtga tgagaatgat
     1021 gttacggcca ttattgttgt gtaaagcagg tatcagaacc tttatgaggg caagatgata
     1081 cagaagcatc tgtaggattg caaaggatgc cccaagctta aaaagggtca cccccggaga
     1141 atttatatta ccaaagaaat tttgcaccaa tgtttgcttt gtatgattgt ctaaaaaaaa
     1201 gagatataag tctccaataa aagctatggc tgcagggata aagcagagat aaaatttttt
     1261 tggaggtttt atctcaggga aggcaactac atagtaggcg aaataaagta ttggggaaaa
     1321 gataaacatt atagttgagt gaaaggaaat tattacaggg taggagagta ttttaaaagt
     1381 tacaacagaa taaaactgca gcaatattat tcccattagt gcaaaaagaa taaagaggtt
     1441 gatgttttcc agtttgcgct tttttacaac taactggcca catgcagtaa ggaagcaggc
     1501 aagtccagaa aaaagtataa ggttatgagc tatatgtctt atcatggtaa atgcatggat
     1561 attgtattag ttagttattg ttttgaccct tttaaagatt taattaaagt ttttcctttg
     1621 tcaaatcttt ttggggtatt taatttgaca ggaaaaatgg ccgtcttata gtgtttatct
     1681 tgttttttta tcaaagataa ggagtttaga atgaaggatt ttgtgaaggc tttttttaag
     1741 aggacagtat tattttccta tttggctttg gtttttacat catttacatt ttgtggggga
     1801 ggtggtgatt cggctgtaga gccgtcgcct gaaaatcagt ggacaataat gtattatgga
     1861 gacggagatt gtgatcttga agaagatctc cttgtagacg ttgaagagat gaaagaagga
     1921 tttgttgatg gccagggtat aaatctcata gttctttttg ataggcatcc agcttatagt
     1981 tctgataaat ctgtctttgg agaagatttt acagatacaa ggctttatcg cataacagca
     2041 aatactgtgt ggaggcttaa tggaggaagt gagttcccag aaatatcaaa gaatggaagt
     2101 tatgaggcta atatgggctc tggtgaaact ttaaaaaaat atcagtatta attccttaac
     2161 ttgattcgag gaataattcc tctatatttg agcctctatc aaacatcctg atagaggatt
     2221 tcagtttgat tatgaaatca tagatagcat ctttcataat acgatatttc tttaaatcat
     2281 tcatctttgc agtaagaacc ataatataag cacataatgc tgtatagatt tgaataagta
     2341 ctccattctt cgacctgctc agccagcgtt caattttgag aaacattttt atccaacgaa
     2401 aaactacttc tatattccat cgctttctat atagttcagc aatctcatca gaacgataac
     2461 tcagtaaatt cgttaataaa gaaaatgtct gttctttacc cgctgaatct ataaatctga
     2521 aagtaaccat cctcatctta tatgttatgt tctgctttct atctgcaatg acctcaataa
     2581 ttcgatctgc aataatacct ttctcttttt gtctatgtcc gacttttcta ttctgcatta
     2641 tattatactt aatattacct aatgctctgg ttacaaaata tgcctgcttt tcttctatcc
     2701 actggaattg gctgtaatca ttataaccgc ggtcgaaaat atggataaat ccttctttta
     2761 cagcccattt tagcgacttt ttatcacttg aattgccatt cttaagcttc acattctccg
     2821 gtatatattc ttcacctatt actgtgtgta acttaaaatc attcttatta cgtctatgct
     2881 ttgcataatc catcgttgct gtagccggaa gtgctgttga atcaataatc cgaattaact
     2941 ttttcatctt ccttatatct tttgatttct taccttgtaa ccggcttaat tgaagtttaa
     3001 acatatcatg aaacaattca accggtaacc tgtaacataa cttctttgat aattcactct
     3061 ggcttattgt tttagttgta tcacctatca tctttttgct atacagacta aatgctcttc
     3121 cattatcaat cccgctatac tgttgcagca gtaataacaa aaataccttt tcaatcggat
     3181 gctttttgat atatttgtta ctatcctttt cctctgataa tttattgaca tcttttacca
     3241 tctgcagtat caattcatca attatcattt tgaaatactc ctcttgtgtg gttctgtggt
     3301 gattccattt caattggagg atttctttat taatactact ttttactcat tgctcctttt
     3361 ttatgcttta ggaattatta ctgaaaaaat ttatagattt ttgtaaagcc catttgttta
     3421 gcttcaatca aatgggaatt tttaccggtt aaattaagcg ggtaaatccc ccattaaaat
     3481 gcgattcaat tcagtgtttc tggtaaatct atcctgccgg gcctgcttca gtttttctct
     3541 tctcgtggca agtatagccg gccctctgcc ataaaaaaca tcaacaggtt ttaaataatc
     3601 aatacctgca tgatacctgt tgttattata ttcatcaaca aatttttcaa ttacttttat
     3661 accctctcca taatatgacg ggctgttagg ccgtagtgct tcagttctga taagtccgac
     3721 aaaacgctca gccttgccgt ttgtct
//
LOCUS       DAOTQL010000143         3726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_19145_length_3726_cov_4.030782, whole genome shotgun sequence.
ACCESSION   DAOTQL010000143 DAOTQL010000000
VERSION     DAOTQL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19145_length_3726_cov_4.030782"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..558
                     /locus_tag="P5293_07540"
     CDS             49..558
                     /locus_tag="P5293_07540"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRR49786.1"
                     /translation="MKRIFLLLLVVLASSAIMRGQSFGYVNSETILNALPQYKTAQQQ
                     IERLKSQFDAEIEKGLKEVEQMFNTYQSQKASLSQNERDQREKEIIMRERAVKDLQKK
                     YFGQDGEMSARSKELLEPIMSKFNAAVEKIANSEGIIIIFDIATSPGIIYNNSAYDLS
                     QKVIKLLNQ"
     gene            580..1161
                     /locus_tag="P5293_07545"
     CDS             580..1161
                     /locus_tag="P5293_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRR49787.1"
                     /translation="MKHLFKPLLLLFALSSSIGTFAQTSLKIGRINSEELIALMPERD
                     SALVKLDKLSKELQENIEAIQNEFQTKYATYQQKQATWTAALLETKQRELEDLQNRFQ
                     QLQQTAPQDFQAEQQKLLTPVYQKANETIKALGKENGCSFVFDLATGAFPYFDESQSI
                     DLLPLARKKLGIPADKVPMQLNAQSAEQSATAK"
     gene            1224..2018
                     /gene="murI"
                     /locus_tag="P5293_07550"
     CDS             1224..2018
                     /gene="murI"
                     /locus_tag="P5293_07550"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRR49788.1"
                     /translation="MNVGIFDSGVGGLSVWREIVKIMPGIPINYFSDSAYCPYGDKSK
                     YEIIDRSLKISDFLISAGAAVIVVACNTATAAAITKLRESFEIPFIGMEPAVKPAALH
                     SQSRVIGVLATKGTLNGDHFHNAVAKYAADVKVIAQVGEGLVELVEAGEIDGDKVQEL
                     LSHYINPMLEEGADNIVLGCTHYPFLIKAIKEITGSGVNIIDPAPAVAKRVKNVMQES
                     HIFIPAAPSPHNSTCFFATSSISILKKMARMIDSEISEMNFIQVDI"
     gene            2042..>3726
                     /locus_tag="P5293_07555"
     CDS             2042..>3726
                     /locus_tag="P5293_07555"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRR49789.1"
                     /translation="MTTEHMFYFVPLASAIALFFAYYFFRQMMKESEGTQSMKDIALY
                     VRQGAMAYLKQQYKVVAIVFLIISVFFAVLAYGFGVQNPWVPFAFLTGGFFSGLSGFI
                     GMKSATYASARTANASMRSLNSGLRIAFRSGAVMGLVVVGLGLLDISLWYIILNYFVD
                     ATGTQKLIVITTTMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKVEAGIPEDDP
                     RNPAVIADNVGDNVGDVAGMGADLYESYCGSILSTAALGAAAFSINLDMQLKAVFAPM
                     LIAAVGIILSIIGIFIVKTKEDVGMSHLLKSLGRGVNFSAIFIGIMTFLILYTLGIEN
                     WFGISFSVISGLVAGVIIGKSAEYYTSHSYKPTRKISESGKTGPATVIISGLGLGMIS
                     TAIPVVTVGLAIILAYCSAINFDIANMLAAKNIGMGLYGIGIAAVGMLSTLGITLATD
                     AYGPIADNAGGNAQMCDLDPEVRKRTDVLDALGNTTAATGKGFAIGSAALTALALLAS
                     YLEEIKIGLVRIGETTIDVAGKVVNVAEATIPDLIEHYQINLMNPKVLTGAFIG"
BASE COUNT         1149 a          644 c          791 g         1142 t
ORIGIN      
        1 actgaaaaat accctgttta tgggtaataa attataaaat atttacgtat gaaaagaata
       61 tttttacttt tacttgttgt attggcctca tcagccatta tgagaggtca atcttttggt
      121 tatgtgaaca gtgagacaat acttaatgct ctccctcaat ataaaacagc tcaacagcag
      181 atagaaagac ttaaatctca gtttgatgct gaaatagaaa aagggcttaa agaagtagag
      241 cagatgttca atacctatca gtctcaaaaa gcatctcttt ctcagaatga gagagaccag
      301 agagagaaag agattattat gagggaacgt gctgttaaag atctgcaaaa aaaatacttt
      361 gggcaggatg gtgaaatgtc tgccaggtca aaggaacttc ttgaaccaat catgtctaaa
      421 ttcaatgctg cggtcgagaa aattgccaat tcagaaggaa ttattattat ttttgacata
      481 gcaacttctc ccgggatcat ttataacaac tccgcctatg atctttcgca aaaagttatt
      541 aaattgctta atcaatagaa actaaataat ttagaaaata tgaaacattt atttaaacct
      601 cttttattac tttttgcctt gtcttcaagc atcgggacat tcgctcaaac ttcacttaaa
      661 attggacgaa tcaattcaga agagttaatt gctttaatgc ctgaaagaga cagtgctctt
      721 gtgaaacttg acaagctgtc aaaagagctt caagaaaata ttgaggcaat tcaaaacgaa
      781 tttcaaacca aatatgctac atatcagcag aaacaggcta catggacagc agcacttctt
      841 gagaccaaac aaagagagct agaagatctg caaaacagat ttcaacaatt gcagcaaaca
      901 gcacctcaag attttcaggc tgagcagcag aaactattaa cacctgtcta tcagaaagct
      961 aatgaaacca ttaaagcttt gggcaaagaa aatggatgtt cttttgtatt tgatcttgct
     1021 acaggtgcat ttccatactt tgacgagtct caaagcatag atcttttgcc tttggcaaga
     1081 aaaaagctcg gaatacctgc agacaaagtt ccaatgcaat tgaatgctca atccgccgaa
     1141 caatctgcaa cagctaaata agcattcaaa tatttttata actttacagt cgttcaattg
     1201 agcgactgtt tttttaatta agtatgaatg taggtatttt tgactccgga gttggagggc
     1261 tttcagtttg gagagaaata gttaaaataa tgccggggat tcccataaat tacttttcag
     1321 acagtgctta ttgtccctat ggtgataaga gtaaatatga gataattgac agatcactca
     1381 aaatatctga ttttcttatt tctgccggag ctgcagtcat cgtagtagct tgtaatacag
     1441 ctacagcagc agcaataaca aaactgaggg aaagctttga aatacctttt ataggaatgg
     1501 aaccggcagt caaacctgcc gcattacatt cacaaagccg ggtcatagga gttcttgcca
     1561 caaagggaac acttaatgga gatcattttc acaatgcagt agctaagtat gctgctgatg
     1621 ttaaggttat tgcccaagtt ggagaaggtc tcgttgagct tgttgaagcc ggagaaatag
     1681 atggagacaa ggtacaagag cttttaagtc attatatcaa tcccatgctc gaagagggag
     1741 cagataacat tgtgcttgga tgtacacatt atcctttttt aataaaggcc attaaagaaa
     1801 ttacaggtag tggagtgaat ataattgatc ctgcacctgc tgtggcgaaa agggttaaaa
     1861 atgtaatgca ggagagtcat atttttattc cagcagctcc aagtccacac aattcaacat
     1921 gtttttttgc tacttcttca ataagtatcc ttaaaaaaat ggccaggatg attgactctg
     1981 agatatctga aatgaatttc atacaagttg atatataata gataaactta actttttaac
     2041 aatgacaact gaacacatgt tctattttgt gccattggct tcggccattg cacttttttt
     2101 tgcctattat tttttcaggc agatgatgaa ggagagcgaa ggcacacagt caatgaaaga
     2161 catcgctcta tatgttagac aaggtgccat ggcatatctt aaacaacagt ataaggttgt
     2221 ggcgatagta ttccttatta tcagtgtttt ttttgctgta ctagcctatg gtttcggagt
     2281 gcagaatccg tgggttccat ttgcatttct tacgggtggt tttttctccg gattatccgg
     2341 atttatagga atgaaatctg caacatatgc atctgcaaga acggctaacg catctatgag
     2401 atcattgaac agtggtttgc ggattgcctt caggtcagga gctgtaatgg gacttgttgt
     2461 tgtaggtctg ggacttttag atatttcact atggtacatt attcttaatt attttgttga
     2521 tgcaaccggc actcagaagc taattgttat tactaccact atgttgacat ttggtatggg
     2581 tgcctcaaca caggctcttt tcgcaagagt tggaggagga atatatacca aagcagcgga
     2641 tgttggagca gatttggttg ggaaggtcga agcgggaata cctgaggatg atcctcgtaa
     2701 tccagccgtt attgccgata atgttggaga taatgttgga gatgtggcag gtatgggcgc
     2761 agacttgtat gaatcttatt gcggttcaat tctatctaca gcagctcttg gtgctgcagc
     2821 tttcagcatc aacctcgata tgcagctaaa agcagttttt gctccaatgt tgatagcagc
     2881 ggttggcatt atactttcaa taataggtat ttttattgtt aagacaaagg aagatgttgg
     2941 tatgtctcat cttttgaagt cgttgggaag gggagtcaat tttagtgcaa tatttatcgg
     3001 tatcatgact tttctaatat tatatactct tggcatcgaa aactggtttg ggatttcttt
     3061 ttcagtcata tcagggcttg tagcgggagt tattattggt aaatctgcgg aatattatac
     3121 atctcattca tataagccaa cccgaaaaat ttcagaaagt ggtaaaactg gtcctgcaac
     3181 tgtgattata tcaggattag gtctgggtat gatttcaaca gccatcccgg ttgttactgt
     3241 gggactggct ataattttag catattgttc tgctattaat tttgatatag ccaacatgct
     3301 tgctgcaaaa aatattggta tgggtcttta tggaataggt attgcggcag ttggtatgct
     3361 ttcgacactc ggaatcacac tggctactga tgcctatggc cctattgccg ataacgcagg
     3421 tgggaatgct caaatgtgtg acctggatcc tgaggttaga aagagaacag atgttcttga
     3481 tgctctcggg aatactacag ctgctacagg taagggattt gcaataggtt cggcagccct
     3541 tacagctctt gccctgcttg catcatatct ggaggagatt aaaattggac tcgttcgtat
     3601 tggagagacc actattgacg tagccggcaa agttgtaaat gttgcagagg cgacaattcc
     3661 tgacttaata gagcattatc agattaacct gatgaatcct aaagttctaa caggtgcatt
     3721 tattgg
//
LOCUS       DAOTQL010000144         3679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_19466_length_3679_cov_4.345199, whole genome shotgun sequence.
ACCESSION   DAOTQL010000144 DAOTQL010000000
VERSION     DAOTQL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19466_length_3679_cov_4.345199"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="P5293_07560"
     CDS             <1..405
                     /locus_tag="P5293_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRR49790.1"
                     /translation="TSEEWREIDKVLEKRDGSSITVSIIYFLLARSVGVEIHPLCFSG
                     GLIPVFTDEKGKLLFYLNLFKGGELLVTKNFDDILRDRGIDFNFNFVKIEEDMALVTI
                     YAELLNLSLEGEKLRRIEKIVALFDGKRYLNP"
     gene            527..1774
                     /locus_tag="P5293_07565"
     CDS             527..1774
                     /locus_tag="P5293_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR49791.1"
                     /translation="MNINLFIAKTLWRKDESRNNAYSGSSTLIAQLSVVISFFIIILS
                     IAITDGFKHEIKSKASGFCGDILLKSPGVEYNSNLYPISGDLSFSNDILAIPEVRSMT
                     GYAYRSGMIRFNDQIQGVIIKGVDADYNRDFFKKIISEGDIIDFSDSTESNSIMLSSR
                     LADMMGFRVGDTVPVYFIDKSVRVSEFKVKGIFDAQLEQIDKTLIIAHINEVQRLNGW
                     SSDQVSGLELQLEAGSDRKLVAERIEKIIFENGSNKDSQLEVLTVDELFPHLFDWLRL
                     LDFNVLIVLVLMMIVAGFNMLSGLLILLFEKISVIGLFKALGMRNMDIHGIFLFKGLN
                     IVFKGMVLGNLLAFAVGLMQKYFHIVKLDPVNYFVKYVPIHFNAVTIILVDIVSLVVI
                     MLLMILPSLFVAKVSPDRLIRVK"
     gene            complement(1761..2822)
                     /locus_tag="P5293_07570"
     CDS             complement(1761..2822)
                     /locus_tag="P5293_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRR49792.1"
                     /translation="MNHRKKFIIIFFLLLICLILSTKKGNIIPDRHVLFFEDDTLNAT
                     VCVKGGVFLKQGHTTGYHYELLKKFSKNQKCQIDVKPFKGTDQWADLIGRYTDILVVD
                     PKDTIPDMYQTEVISSMEINDENHVWVVRKDDILLLQQLNYWIGYYKQTREYRDLNYK
                     YYTKYGSRPVSFSLKNNVISPYDHIIKVYANKINLDWRLLASLICQESKFNPNAKSLR
                     GAQGLMQIKNETARFANIENVFDPEQNIMAGTMLIKRLKTKYEKEGIDSANIIKFILA
                     AYNAGEGRIDDLRAFAIHKNMNPNDWNSIVEVIPMMRQKELLPKGLLKFGTFSGKETV
                     KFVNEVLNRYEEYCAFTSP"
     gene            complement(2830..>3679)
                     /locus_tag="P5293_07575"
     CDS             complement(2830..>3679)
                     /locus_tag="P5293_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49793.1"
                     /translation="NNHYSNSYTYAGASGQFRKYFKWDGMAKFYLSGYNAGDFNIDAG
                     VKFSMYPIDKGIHLTGRVMLSSTSPDWYQEQYCSNHYYWQNNFDKTTESRIEGNLHIP
                     LWDLNAFAGYAIIDNLIYYGLDGVIGQCNNPVTVFSASLEKNLKLWDFHLDNRILYQY
                     TSNKEVLPLPAFSANLRYYMEFDLVKSVMRVQIGADVTYNTEFYAPAYSPALGQFHMQ
                     NDRKTGGYPYVDIFANIQWKRASIFVKYINASHGWPDEDYFSASHYIRPQSALKIGVH
                     WPFYVK"
BASE COUNT         1103 a          606 c          706 g         1264 t
ORIGIN      
        1 acatctgaag agtggagaga aattgacaag gtcctggaaa aaagagatgg gagttctata
       61 actgtatcta ttatttattt tttattagcc agaagtgtag gcgtcgaaat ccatccgtta
      121 tgtttttcag gaggtcttat acctgtattt actgatgaaa aagggaagtt gttgttctat
      181 ttgaatcttt tcaaaggtgg agaattgctg gtaacaaaaa attttgatga cattttgagg
      241 gatcgtggaa tcgattttaa ctttaatttt gttaaaatag aagaagatat ggcattagta
      301 acgatttatg cagaattatt aaacttaagt cttgaaggtg aaaaattaag acggattgaa
      361 aaaatcgttg ctcttttcga cggcaaaaga tatctgaatc cttaatatta cttttaaagg
      421 aaacccccat aatcaaatct tgactatgga ggttctgaag tttagtttta ggttaagtaa
      481 tagctaaata actaatgcaa gacaaatata gtaaaatatt ttaattttga acataaatct
      541 atttatagcg aaaacattat ggagaaaaga tgagagtcga aacaatgcat attccggttc
      601 aagcactctt atagcacaat tatccgtagt tattagtttt tttattatta tattatctat
      661 agccatcaca gatggcttta aacatgagat taaaagcaag gcatccggct tttgtggtga
      721 tattctgtta aaatcacctg gtgttgagta caattcaaat ttatatccta tctctggaga
      781 tctttcattt tccaatgata ttcttgctat tcctgaggtc aggtcaatga caggctatgc
      841 ataccgttcc ggaatgatca ggtttaatga tcaaatacag ggagttatta taaaaggagt
      901 tgatgcagat tataacaggg atttttttaa aaaaattatt tccgaaggag atattattga
      961 tttttctgat tcgacggaga gcaattctat aatgctttca agcagacttg cagatatgat
     1021 ggggttcagg gtaggtgata ctgtgcctgt gtattttatc gataaaagtg tgagggtatc
     1081 cgaatttaaa gtaaagggca tattcgatgc acagttggag cagattgata aaactttaat
     1141 aattgctcac attaacgaag tgcagaggtt aaatggctgg agcagtgatc aggtgtcagg
     1201 attggaactt caactcgagg ccggttcgga tagaaagctt gtagcagaga gaatagagaa
     1261 aattatattt gagaatggaa gtaacaagga ttcacaactt gaagttttaa ctgttgacga
     1321 actttttcca catctttttg actggcttcg tcttttagat ttcaatgttt tgatagtgct
     1381 ggtgttgatg atgattgtgg ctggttttaa catgctgtca ggtttgctta tattgctctt
     1441 tgaaaaaata tcggtaatag gcctctttaa agcattgggg atgaggaaca tggatattca
     1501 tgggatcttt ttattcaagg gcttaaacat tgttttcaaa ggtatggttt tgggaaatct
     1561 cctggcattt gctgtcggac tgatgcagaa atactttcat atagttaaac ttgatccggt
     1621 caattatttc gtgaaatatg tgccgattca tttcaatgct gttactatca tattagtcga
     1681 tattgtgtcc cttgttgtaa ttatgctgct tatgatctta ccttctttgt ttgtggcaaa
     1741 agtaagcccg gacagattaa tcagggtgaa gtaaatgcac aatattcttc atagcggttt
     1801 agtacttcgt ttacgaattt tacagtttct ttaccgctga atgtgccaaa tttcagcaga
     1861 cctttaggaa gtagctcttt ctgccgcatc attggtatga cttctactat tgaattccaa
     1921 tcatttggat tcatgttttt gtgtattgca aatgctctaa ggtcgtcgat tcttccttct
     1981 cctgcattat aagctgccaa aataaactta ataatatttg cagaatcgat tccttctttt
     2041 tcatacttag tctttagcct cttaatcaac attgtgccag ccataatgtt ttgttcagga
     2101 tcaaatacat tttcaatatt tgcaaacctt gcagtttcat tctttatctg catcagaccc
     2161 tgagcccctc ttagactttt agcgttggga ttgaattttg attcctggca tattagagat
     2221 gcaaggagtc tccagtctaa gtttatttta ttggcatata ccttgataat atggtcataa
     2281 ggagatatta cattattttt taaggaaaaa gaaacaggcc tactgccata ttttgtgtaa
     2341 tatttataat ttaaatctct atattctctt gtctgtttgt aatatcctat ccagtaatta
     2401 agttgctgaa gcagcaggat atcatctttt cttactaccc atacatgatt ctcatcattt
     2461 atctccatac ttgaaatcac ttcagtctga tacatgtctg ggattgtgtc ttttgggtca
     2521 acaaccagaa tgtcggtgta tcttcctatg agatctgccc attgatcagt acccttgaaa
     2581 ggtttaacgt ctatttgaca tttctgattt tttgaaaatt tcttaagcaa ttcataatga
     2641 tagccggtag tatgcccttg cttaagaaat acaccccctt ttacgcacac agttgcattg
     2701 agagtgtcat cctcaaagaa caatacatgc ctgtccggaa taatatttcc tttctttgtg
     2761 cttaaaatca gacagattaa taacagaaaa aagattataa tgaacttttt cctatgattc
     2821 atttaaatgt tatttaacat aaaatggcca atgcacacct atcttcaggg ctgattgggg
     2881 cctgatatag tggcttgcag agaaataatc ttcgtcaggc catccatgtg acgcgtttat
     2941 gtatttgaca aatatactag cacgtttcca ttgaatattt gcaaatatgt ctacatatgg
     3001 atatccacct gtcttgcgat cattttgcat gtgaaactga ccaagggcag gactgtatgc
     3061 gggggcataa aactcagtgt tgtatgtaac atctgcgcct atttgcactc gcataacact
     3121 cttgacaaga tcgaattcca tataatatct caaatttgca ctaaatgcag gaagaggtag
     3181 aacttcctta ttagaggtat attgatacag aattctgttg tcgaggtgga aatcccaaag
     3241 cttcagattt ttctctaagc tggcagaaaa gactgtgaca gggttattgc attgtccaat
     3301 tactccgtca agtccatagt aaataaggtt gtcgattatt gcgtaccctg caaaagcatt
     3361 taagtcccaa agaggaatat gaagatttcc ttcaatccgg ctctctgtag tcttatcaaa
     3421 attattttgc caatagtaat gattggaaca gtattgttcc tgataccaat cagggcttgt
     3481 tgacgacaac attactctcc cggtaagatg tatgcctttg tcaatcgggt acatggaaaa
     3541 tttaactcct gcgtcaatgt tgaaatctcc tgcgttataa cctgataaat agaacttagc
     3601 cattccatcc catttaaaat atttgcggaa ctggccggaa gcacctgcat aggtatatga
     3661 attgctatag tgattatta
//
LOCUS       DAOTQL010000145         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_19672_length_3652_cov_4.356964, whole genome shotgun sequence.
ACCESSION   DAOTQL010000145 DAOTQL010000000
VERSION     DAOTQL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19672_length_3652_cov_4.356964"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="P5293_07580"
     CDS             <1..445
                     /locus_tag="P5293_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49794.1"
                     /translation="KTGRDSIVIVNDFRPKVVPTLTIQRPSGYLFPKSDLKLIDWIIR
                     QGYEITPFSVKNPIMEELYIKSIDSIDFERDIIAAANVEEKEIQKSSINIDDYIYVST
                     KQLKGHLLIIGVEPQSELGIATYPQFSYLMKAESIYPVIRIREKQ"
     gene            463..1578
                     /gene="menC"
                     /locus_tag="P5293_07585"
     CDS             463..1578
                     /gene="menC"
                     /locus_tag="P5293_07585"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HRR49795.1"
                     /translation="MKIERIEIRHTKMDLVTPFTTSMGTEYDEEHIMVRIDAEGVTGW
                     GESVAEGTPFYSYETVSTAWHIMRDFLIPALLGKNVSDVEEAIRLYEFARVRGHMMAK
                     AGIEAALWDLMAKSKNLSLSKMLGGVRDKIDVGVSIGIQSSEEELIEEIVGYLSEGYK
                     RIKIKIAPGHDLSLVQAVRREFPDIMLQVDANSAYSLEDANLFKKMDEYNLTLIEQPL
                     GYEDIYDHSKLQRAIKTPICLDESIHSLDDARAAIELKSCRIINIKPGRVGGFTESKF
                     IHDFCMNNEIPVWHGGMLESGIGRAGNVALASLPGFTLPGDISASKKYYKEDIVNPPF
                     IVNPDGTMDVPKGAGIGVEINMKNLDKVTVSKEVFNI"
     gene            complement(1768..1986)
                     /locus_tag="P5293_07590"
     CDS             complement(1768..1986)
                     /locus_tag="P5293_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49796.1"
                     /translation="MAIEAIKEKEILRELSERFNVSIMTISKWKGEFLENSTKVFKNG
                     SSVTDDKEKEIGHFHTRIGQLTMERVFS"
     gene            2200..3360
                     /locus_tag="P5293_07595"
     CDS             2200..3360
                     /locus_tag="P5293_07595"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HRR49797.1"
                     /translation="MKRFIYAFVLVGLLPLIYGCVDTLSSQSSNPDGIVVNCLLNDGE
                     VQTLRLTRSNNVEDLGDYRVVDDAIATLYSHTNTHDIEVGKFVNKGGGEYTLNYKTVP
                     GSSYKLEIITSEGRKLYGMTIMPNAIPVLVYSINGIKEAIQNGEEDIFPDYGCPYSIY
                     GYSGIINSLQLFILKESPLDNALWFCQLNSESDINQPLIAPSDDFKLSSKIYGGSVII
                     DNFNVIDSTTLTNNSTKSTFLLYCRLMPHTDEHYSNSSYWQTGNILFLDSSLSSIFCI
                     RTVSEEYDKYMKSLITKALTFQDETGLSSMFDVSNVYTNIIGGYGIFGAYSEEDIFYD
                     HGQFVNMYFAQSKENILRKDYKDYLVSIPWDEYDNYDNIYIFKEFLWSLQNK"
     gene            3339..>3652
                     /locus_tag="P5293_07600"
     CDS             3339..>3652
                     /locus_tag="P5293_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRR49798.1"
                     /translation="MVTSKQINIAALLLFFLSIVVQTSGQNILDSVVINADKVRVGSL
                     IYTPDKSKIVVSVTGEQDILKYISKLPGVSSGIEGSSSMFVRGGNNGNNKIMLDGVPF
                     YD"
BASE COUNT         1208 a          538 c          706 g         1200 t
ORIGIN      
        1 caaaactgga agagactcga tagttattgt taatgatttt cgtcctaaag ttgttcctac
       61 actcacaatt cagagacctt caggttacct tttccctaaa tctgatttaa aactaattga
      121 ttggattatc agacaaggat atgaaataac acctttttct gtgaaaaacc caataatgga
      181 agaactgtat attaaatcca tagactcaat tgattttgag agagacatta ttgcagcagc
      241 taatgtagaa gagaaagaaa ttcagaaatc ttcaattaat attgatgact atatttatgt
      301 ttctacaaaa caattaaagg gtcatttgct tatcattggc gtagaacctc aatctgaatt
      361 aggaattgca acctaccctc aattttctta tctgatgaag gcagaatcaa tctaccctgt
      421 aataaggata agagaaaagc aataatttat ttactttata ttatgaaaat agaaagaatt
      481 gaaatcaggc atacaaagat ggatctggta actcctttta ccacatcaat gggaacagag
      541 tatgatgagg aacatattat ggtaagaata gacgctgaag gtgtaacagg ctggggtgaa
      601 agtgttgcag aaggtactcc tttctactct tatgaaaccg tatcaacagc atggcacatt
      661 atgcgcgact ttttaattcc ggctcttctg gggaaaaatg tttctgatgt tgaagaagca
      721 ataagacttt atgagtttgc aagggtaagg ggacacatga tggcaaaagc tggaatagag
      781 gctgctcttt gggatttaat ggcgaaaagt aaaaatctct ctctttctaa aatgcttgga
      841 ggtgtcagag acaaaattga cgttggtgtc agtattggga tacaatcctc tgaagaggaa
      901 ctgatagagg aaattgtggg atatctttct gagggatata agagaataaa aatcaagatt
      961 gctcctggtc atgatcttag tttggtacag gctgttagaa gagaatttcc cgatattatg
     1021 ctgcaagttg acgctaattc tgcatattct ttggaagatg ctaatctttt caaaaagatg
     1081 gatgaatata atttgacgct tatcgaacaa cctcttggtt atgaagatat ttatgatcat
     1141 tctaaacttc agcgagctat aaaaacacca atttgtctgg atgaaagtat tcactctctt
     1201 gatgatgcca gagctgcgat agagttgaaa agttgtagga ttatcaacat aaaaccaggt
     1261 agagtaggcg gttttacaga atctaaattt attcatgact tttgtatgaa caatgaaatc
     1321 ccagtgtggc atggaggaat gcttgaatct ggaattggca gggcagggaa cgttgccttg
     1381 gcttctttgc caggatttac tttacctgga gacatttctg caagtaaaaa atattataag
     1441 gaagatattg ttaatcctcc atttattgta aatcctgatg gcacaatgga tgttccaaaa
     1501 ggagccggta ttggagttga aataaatatg aaaaatcttg ataaggtaac tgtgagcaaa
     1561 gaggtattta atatttaaat ctttttagaa attacaacaa aatttagcca attttatttc
     1621 atttggttat ttataatata atactcacca gacctctgta gttggatgtt ccatatgaga
     1681 tgagtcgaaa agcttcataa tcttaggttt tcttcggatt ctcctggttg tcttaaatat
     1741 attttaaacc ggctttcttg cagacactta cgaaaaaacg cgttccattg tcaattgtcc
     1801 gatcctggtg tgaaaatgcc caatctcttt ctctttgtca tctgtgaccg atgagccatt
     1861 cttaaaaact ttggtactgt tctccaaaaa ttctcccttc catttgctta ttgtcatgat
     1921 gcttacattg aacctctcag acaactccct taatatctct ttttctttta ttgcctctat
     1981 tgccatcctg gctttgaata aggctgaata agcgtaaaat taaatactga ttagttgctc
     2041 cgcaagtggt ctcgttttgg aggagtatta aagtattata taaaagcctt atctattatt
     2101 ttagtgcaaa taaaatgcaa tttaggtttt ggtaggatta atttattaaa tttgaaataa
     2161 ttagtaaatt gattcaaatt tcattttaaa gcaaatttta tgaaaaggtt tatttatgca
     2221 tttgttttgg tgggtttgct tcccttaata tatggttgtg tagacacgtt atcatcacag
     2281 tcaagtaatc cggatggcat agtagtaaat tgccttctaa atgacgggga agttcagact
     2341 ctcaggctca cgagaagcaa taatgtggag gatttagggg attacagagt agtagatgat
     2401 gctattgcca cattatattc tcataccaac actcatgata ttgaggttgg aaagtttgtc
     2461 aataagggag gaggtgagta tacattaaat tataagaccg tacctggctc atcatacaaa
     2521 ttagagataa ttacatcaga agggagaaaa ttgtatggca tgactataat gccaaatgca
     2581 atacctgttc ttgtatatag tataaacgga attaaagaag caatacaaaa tggagaagaa
     2641 gatattttcc cagattatgg ttgtccttat agtatttatg gatattcagg tataattaac
     2701 tctttacaac tatttatctt gaaagagtcc ccattggaca atgcattatg gttctgccag
     2761 ttgaatagtg aatcagacat caaccaacca cttatcgctc caagtgatga ttttaagctt
     2821 agctctaaaa tttatggtgg atcagtaatt atagataatt tcaatgttat tgatagtacc
     2881 acactgacaa ataattctac aaaatcaaca tttttgctat attgtagact aatgccacat
     2941 actgatgaac attattcaaa tagttcttac tggcaaacgg gaaatattct ctttttagat
     3001 agttctttaa gtagtatttt ttgtataagg acggtttccg aagagtatga caaatatatg
     3061 aaaagcctta taactaaggc attaacattt caggatgaga caggattaag ttcgatgttt
     3121 gatgtgagta acgtatatac aaacataata ggaggttatg gtattttcgg tgcctattct
     3181 gaagaggata ttttctatga tcatggtcag tttgttaata tgtatttcgc tcagagcaaa
     3241 gaaaacatac ttagaaagga ttacaaagat taccttgtaa gcattccatg ggatgaatat
     3301 gataattatg ataatattta catatttaag gaatttttat ggtcacttca aaacaaataa
     3361 atatagcagc actgttgttg ttttttttaa gcattgtggt gcaaacaagt gggcagaata
     3421 ttcttgattc tgtagttata aatgccgata aagtaagagt tggatcttta atttatacac
     3481 ctgacaagtc taaaatagtt gtttcagtga ccggagagca ggatatctta aaatatatat
     3541 caaaattgcc tggtgtttca agtggaatag aaggcagttc ctcaatgttt gtaaggggag
     3601 gaaataacgg aaataataaa ataatgcttg atggagttcc tttttatgac tg
//
LOCUS       DAOTQL010000146         3647 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_19706_length_3647_cov_4.748051, whole genome shotgun sequence.
ACCESSION   DAOTQL010000146 DAOTQL010000000
VERSION     DAOTQL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19706_length_3647_cov_4.748051"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="P5293_07605"
     CDS             <1..1676
                     /locus_tag="P5293_07605"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRR49799.1"
                     /translation="VDIEKVKEAIKEEDCVFLAKTTMFACGDSNQKEMESDIKENNLS
                     GIVVASCSPKLHLNTFRAVSERAGLNKYNYVHANIREQASWAHSDDKIGATEKAIHIV
                     KAAIAKARYAQSLFPIKIESEKSVAVIGAGVSGLKASLALSKMGDKVYLIEREDKVGG
                     KLTTLGKIFPSDIQGSDFVRRLEEEVKKENNITLITNAKIEKCTGNIGHFQIVVATNK
                     DENRNENNHEEINVGSILVATGFESYSPQEGEFGYNNVNNVITLPEFRNLLRENNGHK
                     NLLYNNSKVNNIAFIYCVGSRQIRGENKYCSRYCCSAAMNTSLELKDKYSDVQIYHFT
                     KGVRTYGAQELMYAEACRKGDIFIQYPDKEEPVVNKDGDHVIVRAKDLLTSKREIEVL
                     ADLVVLITGMVPDSDKSLGSMLKLPRGRDNFFNEIHVKLRPVETVIDGITIAGTCQGP
                     KGVMESVNSALSAAVKSHSFVSKGELELPPIVAHVDTTTCSWCDACSNACPFNAVIMT
                     TVNNKRVADINSTTCKGCGMCLPVCPSDSIELTSFTNKEIESMIDILAS"
     gene            1679..1960
                     /locus_tag="P5293_07610"
     CDS             1679..1960
                     /locus_tag="P5293_07610"
                     /inference="COORDINATES: protein motif:HMM:NF017402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49800.1"
                     /translation="MDNIKKQTVKIRRENFGVSDESMKNYKEFNRIKKMILEALSKED
                     LTIASLSEKTGFPKDKILFYLLSLVKFGLVETGDIDEMDEYYKYKLKKQ"
     gene            1957..3015
                     /locus_tag="P5293_07615"
     CDS             1957..3015
                     /locus_tag="P5293_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRR49801.1"
                     /translation="MTRIDPEFGKRLKKYGAFDYDACYNCGTCTSVCSLAKKDEEFPR
                     EMVRLSAIGAKKEIQSSLKPWLCYYCGECSTNCPRSANPGELMMSLRRWLIASYDWTG
                     ISGLLFRSLTATVVALTIVAVAVIVFAITMGFDTLTIMRYGHYFEIGAVTGVFAFILL
                     PNLMRMWYFTIIRPGYKISVRNYAMSLKELFIHMFTQKRSLGCDNNQKRWFEHFILVI
                     GYLTLLFTTVFLDWFGTESTAIIITGYVVSAIIFIVTADFVSGRIKKNKEISKHSRPS
                     DWLFVIWLCLMGLTAFIVRGAIDLGILEQNLWIYLIHLTVLAQWALIIVPFGKWTHFI
                     YRSFALYFAKLIYNTQTK"
     gene            complement(2954..>3647)
                     /locus_tag="P5293_07620"
     CDS             complement(2954..>3647)
                     /locus_tag="P5293_07620"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRR49802.1"
                     /translation="DNFASIVRAVRMGRRIFDNLQKAFSYIFAIHVPIAGLSLIPVFF
                     KDMPLLLWPVHIVFLELIIDPACSTIFEAEKEEKDIMDRPPRNVDEPFFGFKKIALSC
                     TQGISVLICTLLVFLVGMMLGYSDGSVRAMSFVTLIASNIAIIFSNRSWSENIFKIIR
                     TPNNTVKWVVGGAVFFLVLVLNIPFLRELFQFERISFVEGIVCTVVGLISIIWFECYK
                     SILQNRAQKTCK"
BASE COUNT         1261 a          628 c          710 g         1048 t
ORIGIN      
        1 atgtggatat tgagaaagta aaagaggcca ttaaagaaga agattgtgtt tttttagcca
       61 aaacaacaat gtttgcatgt ggtgactcca atcagaaaga gatggagtca gacatcaagg
      121 aaaataatct cagcggtatt gttgttgcat catgttcacc taaacttcac ctcaacacat
      181 tccgtgcagt aagtgaaaga gccggactaa acaaatataa ttatgttcat gccaacattc
      241 gtgaacaagc ctcctgggct cactccgacg acaagatcgg tgcaacagaa aaagcaatac
      301 atattgttaa ggctgcaata gctaaggcaa gatatgcgca atcacttttc ccaataaaga
      361 ttgaatctga aaaatccgtg gcagtaatag gtgcaggagt ttcaggtctt aaagcatctc
      421 ttgcactttc caaaatggga gacaaggtat atctcattga gagggaggat aaagttggag
      481 gtaaacttac aacattgggc aaaattttcc cctccgacat tcaaggaagt gattttgtaa
      541 gaagactcga agaagaagtt aaaaaggaaa ataatattac tcttattacc aacgccaaaa
      601 tagaaaaatg cacaggaaat attggtcatt ttcaaattgt tgtagccact aacaaagatg
      661 agaacagaaa tgaaaataat catgaagaga ttaatgtagg ctctattctg gttgctacgg
      721 gatttgaatc ctacagtcca caagaaggtg aatttggtta caacaatgtt aataatgtaa
      781 ttacccttcc tgaatttaga aacttactta gagaaaacaa cggccataaa aatttattat
      841 acaacaactc caaagtaaat aatattgcat ttatatattg cgtaggaagt cgtcagattc
      901 gaggcgaaaa caaatactgt tcaagatatt gttgctcagc agccatgaat acctcactag
      961 aattgaaaga caaatattcc gacgttcaga tttatcactt tacaaaggga gtaagaacat
     1021 atggagcaca ggaacttatg tatgctgaag catgcagaaa aggtgatatt tttattcaat
     1081 atccagataa ggaggagcct gttgttaata aagatggcga ccatgtaatt gtcagagcca
     1141 aagatttatt aacttctaaa agagaaattg aagttcttgc tgatcttgtg gttttaatca
     1201 ctggtatggt tcctgacagc gataaatcat tggggtcgat gttaaaactg ccaagaggaa
     1261 gagataattt cttcaacgaa atacatgtaa agcttcgccc agttgaaact gtaattgacg
     1321 ggataacaat tgccggaaca tgccaaggac caaaaggcgt aatggaatct gtcaactcgg
     1381 ctctttctgc ggctgttaaa tctcactcat ttgtaagtaa aggagagctg gaacttccac
     1441 caattgtagc tcatgtagat acaactacat gcagctggtg tgatgcatgc agtaacgcat
     1501 gtccgtttaa tgcagtaatt atgactaccg taaataataa aagagtagct gatatcaatt
     1561 caacaacctg caagggatgt ggcatgtgcc tgccagtatg cccttcagat tctatagaac
     1621 taacatcatt tacaaataag gaaattgaaa gtatgattga tattctagca agttaaagat
     1681 ggataacatt aaaaaacaga cagtaaaaat acggagagag aactttggag tatcagacga
     1741 atcaatgaaa aattacaaag agttcaaccg tatcaaaaaa atgattcttg aggcattgag
     1801 taaagaagat ctcacaattg cctcactcag cgaaaaaaca ggttttccaa aggacaagat
     1861 tttattttat cttttgtcat tagttaaatt tggattggtt gagacaggtg atattgatga
     1921 aatggacgaa tactataaat ataaattgaa gaaacaatga ccagaataga tcctgagttc
     1981 ggtaaaagac ttaaaaagta tggagcattt gactatgatg catgctataa ctgtggaaca
     2041 tgtacttctg tctgttctct cgctaaaaaa gacgaagagt ttccaaggga gatggtcaga
     2101 cttagtgcaa ttggagcaaa aaaagagatt caatcttcac ttaaaccatg gttgtgctac
     2161 tattgcggag aatgctctac aaactgtcca agaagtgcca acccaggaga attgatgatg
     2221 tccttacgaa ggtggttaat tgcaagttat gactggacag ggatttcggg attgttattc
     2281 agatcattaa cggcaactgt cgttgctcta actattgtgg cagttgcagt aatagtattt
     2341 gccattacga tgggatttga cactttaaca attatgagat atggccatta ttttgaaatt
     2401 ggagctgtta caggtgtttt tgcttttatt ttgcttccca atttaatgag gatgtggtat
     2461 ttcacaataa ttcgacctgg ttacaaaata tctgtaagaa attatgccat gtctcttaaa
     2521 gagcttttta tacatatgtt cacccaaaaa agatcccttg gttgtgataa taaccaaaag
     2581 agatggtttg aacattttat tctggtaatt ggctatctca cactgctttt tacaaccgtt
     2641 ttcctggatt ggttcggaac agaaagcact gcaataataa tcacaggcta tgtggtaagt
     2701 gcaataatct tcatagtgac agcagatttt gtatcaggaa ggattaagaa aaacaaggag
     2761 ataagtaaac actcgagacc ttcagactgg ctatttgtta tttggctgtg tctaatggga
     2821 cttactgcct ttattgtcag aggtgcaata gatttgggaa ttcttgaaca aaatctgtgg
     2881 atctacctga ttcatcttac agtacttgcc caatgggctc ttatcattgt tccatttgga
     2941 aaatggacac attttattta caggtctttt gcgctctatt ttgcaaaatt gatttataac
     3001 actcaaacca aataatactg attaatccaa ccactgtaca aactatccct tcaacaaaac
     3061 taatccgttc aaactggaac agctccctta gaaatgggat attcaggact aaaactaaaa
     3121 agaagacagc tccccctact acccatttaa ctgtgttatt gggtgtgcgt attattttaa
     3181 aaatattttc actccatgat ctgttagaaa atattatcgc aatattagat gcaataagtg
     3241 tcacaaaaga cattgccctt acggagccat cgctgtatcc aagcatcatc ccaacaagaa
     3301 aaaccagaag ggtacatata agtactgaga taccttgagt acaactcaaa gctatctttt
     3361 taaagccaaa aaatggttcg tcaacatttc tgggtggtct atccattata tctttctctt
     3421 ctttctcagc ttcaaaaatt gttgaacatg caggatcaat gatcaactca agaaatacta
     3481 tatgcacagg ccagagaagc agaggcatat ctttaaaaaa gacaggtatt agcgatagtc
     3541 cggcaatagg aacatggatt gcaaaaatat agctgaacgc tttttgaaga ttgtcgaaaa
     3601 ttctccttcc cattctaact gctctaacaa ttgacgcaaa attgtcg
//
LOCUS       DAOTQL010000147         3603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_20023_length_3603_cov_4.764374, whole genome shotgun sequence.
ACCESSION   DAOTQL010000147 DAOTQL010000000
VERSION     DAOTQL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20023_length_3603_cov_4.764374"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="P5293_07625"
     CDS             <1..423
                     /locus_tag="P5293_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49803.1"
                     /translation="SWGILVTQEGGYQFFPGLARGEGFYFALVQKPLEKGAAKHSAPF
                     SRLSPVTSTVSTFLKPSHEYALSICYGNEWPSIELSRQEALNYLSRNSVKLKESAPLG
                     YLRVTFMGLGLGFVKNLGNRVNNLYPVNRRILYPPKSS"
     gene            complement(402..1649)
                     /gene="nqrF"
                     /locus_tag="P5293_07630"
     CDS             complement(402..1649)
                     /gene="nqrF"
                     /locus_tag="P5293_07630"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HRR49804.1"
                     /translation="MDITTIITVAVLAILIVTLILVAMLLYAKAKLTPSGKVKITING
                     EKELSVSPGSSLLTTLSNEKIFLPSACGGSGTCGMCKCRVIKGGGEILPTETGFFTRK
                     QQKNNWRLGCQVKVKEDLEIIIPEEVLGIKKWECEVISNRNVATFIKEFIVKLPEGEN
                     LKFRSGGYIQIDIPPCEVDFSKDIYVEPKFREDWDRMKIWDLKMVNKENTFRAYSMAN
                     HPAEGNIIMLNVRIATPPWDRTKGTFMNVNPGVCSSYIFSRKPGDKVTISGPYGEFFI
                     KDTQNEIIFVGGGAGMAPMRSHIFHLFKTEKTTRKVSYWYGARSLREVFYEEDFRQIE
                     REFPNFTFHLALSEPRPEDNWTGLTGFIHQALYDNYLKYHETPEDVEYYLCGPPMMNS
                     AVINMLDNLGVPPENIAYDDFGG"
     gene            complement(1663..2280)
                     /gene="nqrE"
                     /locus_tag="P5293_07635"
     CDS             complement(1663..2280)
                     /gene="nqrE"
                     /locus_tag="P5293_07635"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HRR49805.1"
                     /translation="MQELVNIFVKSIFVENMVFAFFLGMCSFLAVSKSVKTASGLGIA
                     VIFVLVVTIPVNYLLYEYVLKPGALVWLNPDYASLDLSFLSFIIFIAVVASITQLVEM
                     VVEKFTPALYSSLGIFLPLIAVNCAILGGSLFMQERGYETLAQATTYALGSGIGWALA
                     IVSLAAIREKLKYSNVPKPLRGLGITFIMVGLMGIAFMSFMGIKL"
     gene            complement(2293..2913)
                     /locus_tag="P5293_07640"
     CDS             complement(2293..2913)
                     /locus_tag="P5293_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HRR49806.1"
                     /translation="MDNKIYIDPLFKKNPITVLVLGICSSLAVTVKVEPAIVMAISVT
                     IVTGFSNLILSLLRNTIPNRIRIIVQLVVVAMLVILVDQILKAYSYDVSKQLSVFVGL
                     IITNCIIMGRIEAFALGNKPIPSLVDGLANGAGYGIILVIISIIRELLGSGTLFGIQI
                     IPLNWYASNGGFYYNNGLMILPPMALILIGLIIWVQRSFDKDLQEK"
     gene            complement(2922..3566)
                     /gene="nqrC"
                     /locus_tag="P5293_07645"
     CDS             complement(2922..3566)
                     /gene="nqrC"
                     /locus_tag="P5293_07645"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HRR49807.1"
                     /translation="MDTNKNLYTIIYATVLVVLVAVILALASYLLKDRQQRNVSIETK
                     QMILKAVHLAQDPEKLKKNKDNYIESEYAKYIKDTIITTGSKTLPLFICNIDSSRYYI
                     VPLHGTGLWGPIWGYIALKSDFNTVYGAVFDHKGETPGLGAEIATAKFQEQFKDKQIF
                     EGDNFISISVVKGGAEKGNPHQVDAISGGTITSRAIESMLKTCISEYLDFFKNK"
BASE COUNT          972 a          714 c          773 g         1144 t
ORIGIN      
        1 agttggggaa tattagtgac tcaagaaggg ggatatcaat ttttcccagg tctcgcaaga
       61 ggagaggggt tttattttgc tcttgtccaa aaacctttgg agaaaggggc tgcaaaacat
      121 tctgctccat tttccaggtt gtcacctgta acatcaacag tgtcaacatt tttgaagcct
      181 tctcatgaat atgctctttc aatttgttat ggtaatgaat ggccttcaat agaactctct
      241 cgtcaagagg ctcttaatta tctttcaagg aactctgtaa agcttaaaga aagtgcccca
      301 cttggttatt tgagagtaac tttcatgggg ctggggcttg ggttcgtcaa aaatttaggt
      361 aatagagtga ataatcttta tcccgttaac aggagaattc tctatcctcc aaagtcgtcg
      421 taggctatgt tttcaggagg gactccaaga ttgtcgagca tgttgataac agcactgttc
      481 atcattggag ggccacacag atagtactct acatcttcag gtgtttcgtg atatttgaga
      541 taattgtcgt aaagtgcctg gtggataaat ccggtaagac ctgtccaatt gtcttcgggt
      601 cttggctcac ttaatgcaag gtgaaaagtg aaattaggga attctctctc tatttggcgg
      661 aaatcttcct cgtagaaaac ttcgcggaga gaacgtgctc cataccagta gcttaccttg
      721 cgtgtggtct tttctgtctt gaaaagatgg aagatgtgag aacgcatcgg agccattcct
      781 gcacctcctc cgacaaaaat tatttcgttc tgggtgtctt ttatgaagaa ttcgccatat
      841 gggccggaaa ttgtaacttt gtctccgggc ttgcgtgaga agatgtacga tgagcaaacg
      901 ccgggattga cgttcatgaa tgttcccttg gtacggtccc agggtggagt tgcaatgcgg
      961 acattgagca ttataatgtt gccttcggca ggatgattgg ccattgaata ggctctgaag
     1021 gtattttcct tgttgaccat tttaagatcc caaatcttca ttctgtccca atcttcacgg
     1081 aatttgggtt ctacatatat atccttgcta aaatcaactt cacatggagg aatatctatt
     1141 tgaatatatc ctcctgacct gaatttgaga ttttctcctt ccggaagttt gacgatgaac
     1201 tcttttatga atgtagctac attacggttg ctgataactt cgcactccca tttctttatt
     1261 ccgagcactt cctctggaat tatgatctcc aggtcttcct tgaccttgac ctggcatccg
     1321 agacgccagt tgtttttttg ttgcttgcgg gtgaaaaaac ctgtttctgt aggaagaatt
     1381 tctccacctc ccttgataac acggcatttg cacatgccgc atgtgccgct tccgccgcag
     1441 gcagacggaa gaaaaatctt ctcgttggag agtgttgtaa gaagtgaaga acctggactt
     1501 actgataatt ctttttctcc gtttattgta atttttacct tgcctgaggg ggtcaatttg
     1561 gcttttgcat aaagcaacat tgccaccaaa atcaaggtaa cgataagtat tgcgagtact
     1621 gctacagtaa ttattgttgt tatatccatg atctcttctc tgttacagtt taattcccat
     1681 aaagctcatg aatgctatac ccatgagtcc taccataata aatgttattc ccagtccacg
     1741 aagtggcttt ggtacgtttg aatatttaag tttttcgcgt atggctgcca gtgaaactat
     1801 tgcaagtgcc cagccaattc ctgaaccgag agcatatgta gtagcctgcg caagtgtctc
     1861 ataacctctt tcctgcatga agagtgatcc gccaaggatg gcacagttga cggcaatgag
     1921 aggaagaaag attccaagtg aggagtaaag tgctggtgta aacttttcta caaccatctc
     1981 tacaagttgt gtgattgagg ctacaacagc tataaatatg ataaaactta aaaagctgag
     2041 atcgagactg gcgtaatcag gatttagcca caccagtgct ccaggtttga gtacatattc
     2101 ataaaggaga tagttgacag gtatagtcac tacaagaaca aatatgactg ctattccaag
     2161 acctgaggcc gttttgacac tttttgaaac tgccagaaat gaacacattc ccagaaagaa
     2221 ggcaaaaacc atattttcaa cgaatatgga ttttacgaaa atattaacta actcttgcat
     2281 agtggtatgt gtttattttt cttgtaaatc cttgtcaaaa cttctttgca cccaaatgat
     2341 gagtccgatt aaaatcaaag ccatcggagg cagaatcatc aatccgttgt tgtaatagaa
     2401 tcctccgttg gaggcatacc agttgagggg aataatctga atgccaaaga gtgttcctga
     2461 accgaggagt tctcttatga ttgatataat tacgaggatt attccatagc cggcgccatt
     2521 tgccagacca tcaacaagtg agggtattgg tttattgcca agtgcaaatg cttcgatacg
     2581 tcccatgatt atgcagttgg ttattatcag acctacaaag accgataatt gtttgctgac
     2641 atcataggaa taagctttca aaatctggtc gacaagaatt acgagcattg caactactac
     2701 gagctggaca atgatcctga tccggtttgg aattgtgttt ctgagtagtg acaggatcag
     2761 attggaaaat cctgttacta ttgttactga aatagccatg actattgcag gttctacctt
     2821 gacagtgacc gcaagtgaag aacatatgcc aagtactaga actgtaattg ggttcttctt
     2881 aaacagcggg tctatgtata ttttattgtc catagttcaa cttatttatt tttgaaaaag
     2941 tcgagatatt cacttataca ggttttgagc atggattcga tagctctgct cgtgattgta
     3001 ccccctgaga tggcatctac ctgatgaggg tttccttttt cagcacctcc cttgacgaca
     3061 ctgattgata taaaattgtc gccttcgaat atctgcttgt ctttaaattg ttcctgaaat
     3121 ttggcggtag caatctctgc tccgagtccc ggtgtttctc ctttgtggtc gaatactgcg
     3181 ccataaacag tattgaaatc acttttaagt gcaatgtatc cccagattgg tccccacagc
     3241 cctgtgccat gaagaggaac aatgtagtat cttgatgaat cgatattgca aataaagagt
     3301 ggaagggttt tgctgcctgt tgttattatt gtgtctttga tatatttagc atattcactt
     3361 tcaatatagt tatccttgtt ttttttgagt ttttctggat cctgggcaag atgaactgct
     3421 ttgagtatca tttgcttggt ctcaattgag acgttccgct gctgcctgtc cttgagcagg
     3481 tatgatgcca gggcaaggat tactgctact aatactacca gaactgtggc atagattatt
     3541 gtgtacagat ttttatttgt gtccattgta atcctcccta ttatttgaca attactgcgg
     3601 ctc
//
LOCUS       DAOTQL010000148         3592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_20098_length_3592_cov_3.633305, whole genome shotgun sequence.
ACCESSION   DAOTQL010000148 DAOTQL010000000
VERSION     DAOTQL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20098_length_3592_cov_3.633305"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /gene="rplJ"
                     /locus_tag="P5293_07650"
     CDS             complement(<1..306)
                     /gene="rplJ"
                     /locus_tag="P5293_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRR49808.1"
                     /translation="MRKEEKIQIIENLAAQLNQTPNFYLTDISGLNAETITKLRRACF
                     EKKIKLVVVKNTLLRKTLEQKGINEAEKLFPVLEGPSAVMFTEEVNAFVMDYGSSYIK
                     "
     gene            complement(318..1019)
                     /gene="rplA"
                     /locus_tag="P5293_07655"
     CDS             complement(318..1019)
                     /gene="rplA"
                     /locus_tag="P5293_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRR49809.1"
                     /translation="MSKLTKNQKEAYAKVDSTKQYKLADACSLVKEVSFTKFDASVDI
                     AVRLGVDPRKANQMVRGVVTLPHGTGKNVRVLVLCTPDKENEAKEAGADYVGLDEYID
                     KIKGGWTDVDVIICTPSVMAKIGPIGRILGPRGLMPNPKTGTVTMDVGNAVKEVKSGK
                     IDFRVDKQGIVHASIGKVSFPAEKLTENALEFIKTVIKLKPQALKGTYIKSIYISSTM
                     SPGICVDIKSIGAAE"
     gene            complement(1033..1479)
                     /gene="rplK"
                     /locus_tag="P5293_07660"
     CDS             complement(1033..1479)
                     /gene="rplK"
                     /locus_tag="P5293_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRR49810.1"
                     /translation="MAKEIAALIKLQIKGGAANPSPPVGPALGSKGVNIMDFCKQFNA
                     RTQDKAGKVLPVIITVYSDKSFTFIVKQPPVAVQLKEAAKIQSGSAEPNRKKVGSVTW
                     EQVRAIATDKMPDMNAHTLKSAMTMVAGTARSMGITVTGDFPEDVK"
     gene            complement(1492..2052)
                     /gene="nusG"
                     /locus_tag="P5293_07665"
     CDS             complement(1492..2052)
                     /gene="nusG"
                     /locus_tag="P5293_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRR49811.1"
                     /translation="MSEITKKWYVVRAAGGKEKKAKEYIENEIKRLGLSDYVSQVLIP
                     TEKIYQVKNGKKVSSERIFYPGYILIEAALTGEIQHIIRNIPHVSGFLSEKAGAGKDK
                     EPVPLRMSEVNRILGRVDELVDMEEENVTPFIIGEPVKVIDGPFNGFDGTVEEIMEDK
                     KKLKVMVKIFGRKTLLELNFVQVVKE"
     gene            complement(2058..2252)
                     /gene="secE"
                     /locus_tag="P5293_07670"
     CDS             complement(2058..2252)
                     /gene="secE"
                     /locus_tag="P5293_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRR49812.1"
                     /translation="MNKIKLYFKESYTELVKKVSWPTWSQLQSSAIVVMIASVVIAVV
                     IFAMDFAFRNVMTFIYNLLY"
     gene            complement(2335..2405)
                     /locus_tag="P5293_07675"
     tRNA            complement(2335..2405)
                     /locus_tag="P5293_07675"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2371..2373),aa:Trp,seq:cca)
     gene            complement(2455..3591)
                     /gene="tuf"
                     /locus_tag="P5293_07680"
     CDS             complement(2455..3591)
                     /gene="tuf"
                     /locus_tag="P5293_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRR49813.1"
                     /translation="MGHVDHGKTTLTAAITKVLARKGLSEVRSFDSIDNAPEEKERGI
                     TINTSHIEYQTEKRHYAHVDCPGHADYVKNMVTGAAQMDGAILVVAATDGPMPQTREH
                     ILLARQVNVPRIVVFLNKVDLVDDPEMIDLVEMEVRELLSFYKYDGDNAPVIRGSALG
                     ALNGDPKWEEKVVELMDAVDEYIPIPPRDIDKPFLMPVEDVFSITGRGTVATGRIETG
                     VVKTGEEVQIIGLGEEPKKTVVTGVEMFRKILDRGEAGDNVGLLLRGIDKKEVKRGQV
                     IAKPGSITPHKRFKAEIYVLKKDEGGRHTPFHNHYRPQFFIRTMDITGEIMLPEGVEM
                     VMPGDNVTITVELIYPVAINQGLRFAIREGGRTVGAGQVTEILD"
BASE COUNT         1005 a          843 c          668 g         1076 t
ORIGIN      
        1 ttttatgtaa ctacttccat aatccattac aaaagcattt acttcttctg taaacattac
       61 tgcagaagga ccttcaagga caggaaaaag cttttcagcc tcgtttatgc ctttttgttc
      121 caaagtttta cgcagcaagg tgtttttaac gacaacaagt tttatcttct tttcgaaaca
      181 cgcacggcgt agcttagtta tagtttcagc attcagtcct gatatgtcag tcaaataaaa
      241 atttggagtt tgatttaact gcgctgccag gttctcaata atctgtattt tctcttcttt
      301 tctcataagc aatttaatta ttcagcagca cctattgact taatatcgac acatattcca
      361 gggctcatgg tggatgatat atagatactc tttatataag ttcccttcaa agcctgtggt
      421 ttcaacttaa tgactgtttt gatgaattcg agagcgtttt cagtcaattt ctcggcaggg
      481 aaagagacct tacctatact ggcatgcact atcccctgtt tatcaaccct aaagtctatt
      541 ttaccagact ttacctcttt aacagcattt cctacatcca tggtaacagt gcctgttttt
      601 gggttaggca tcagaccgcg tggaccaaga atacggccaa taggaccaat cttggccatc
      661 acagatggag tacatataat gacatctaca tcagtccatc caccctttat cttatcgata
      721 tattcatcca gaccaacata gtcagcacct gcctcttttg cctcattctc cttgtcagga
      781 gtacaaagca ccagaacacg aacattttta cctgttccgt gagggagtgt aacaacaccg
      841 cgtaccatct ggttcgcttt acgtgggtca actccaaggc gaacagctat gtcgacggag
      901 gcatcaaatt tagtaaaaga aacctctttt acaagactgc atgcgtctgc caatttatac
      961 tgcttggtag agtcaacttt agcatatgct tctttttgat tttttgttag cttactcatt
     1021 ttcgcaggtt tattatttaa catcttccgg aaaatctccg gtaacagtga tacccatact
     1081 tcttgcagta ccggcgacca tagtcatagc tgacttgaga gtgtgagcat tcatgtcggg
     1141 catcttgtcg gtggcaatag cacgaacctg ttcccaggtc actgagccaa cctttttacg
     1201 gttaggttcg gcagaacctg attgaatttt ggcagcttct ttcaactgaa cggcaacagg
     1261 aggttgttta acgataaaag taaaagattt atcgctgtaa acagtaatta ttacaggcaa
     1321 caccttgcct gccttgtcct gagtgcgagc attgaattgc ttgcaaaaat ccattatgtt
     1381 tacacccttg gagccgagtg ccggccctac aggaggtgaa ggattagctg caccgccttt
     1441 gatctgcaat ttaatgagag cagcaatttc tttagccata atgtttatta gttattcttt
     1501 aacgacttga acaaaattta attcgagaag agttttcctt ccaaaaatct taaccatcac
     1561 tttcagcttc ttcttatcct ccatgatctc ctcaacagtt ccgtcgaaac cgttaaaagg
     1621 gccatctata acctttacag gctctccgat gataaatggt gtgacatttt cctcttccat
     1681 atcgacgagt tcatcaacac ggcctaaaat acggttgacc tcagacattc tcaaaggaac
     1741 gggttcctta tccttgcctg cacctgcctt ctcagacagg aaacctgata catgaggaat
     1801 gttcctgata atatgctgga tctcccctgt aagagcagct tcaataagga tatatcccgg
     1861 atagaaaatt ctttccgagc taaccttttt gccgtttttg acctgataga tcttttcggt
     1921 aggaatcagt acttgagaca cataatcaga gagacccaat cttttgatct cgttttctat
     1981 gtactctttt gcctttttct cctttccacc ggcagctctt acaacatacc actttttggt
     2041 aatttcactc ataagactta gtagagcaaa ttgtaaataa aagtcataac gtttctgaaa
     2101 gcaaaatcca tcgcaaaaat cacaactgct atgacaacag aggcaatcat cacaactata
     2161 gcggaactct gaagctgaga ccatgtaggc cagctaacct ttttaacaag ttctgtgtac
     2221 gattctttaa agtacaactt aattttgttc attttacaac aattgtcggc cgaaacaacg
     2281 gaagctaatt gcctcaaccg ggttatttct accggatcgt aacccccggt attgcagggg
     2341 cagagcgatt cgaacgccca tcaacggttt tggagaccgc tattctaccc ttgaactatg
     2401 cccctgaata taaagttagc caccagggag gtgactaact tttaaacatt caaatcagtc
     2461 gagaatctct gtaacctgac cggctccaac tgtacggcca ccctcgcgga ttgcgaagcg
     2521 aagaccctga ttgatagcta caggatagat aagctctact gttattgtaa cattatctcc
     2581 aggcataacc atctctactc cctcaggaag cataatttca cctgtaatgt ccattgtacg
     2641 aataaagaac tgaggacggt agtggttgtg gaaaggagtg tgacggccac cctcgtcctt
     2701 cttaagaacg taaatctcag ccttgaatct cttgtgaggt gtaatactgc caggctttgc
     2761 aataacttgt cctctcttaa cttctttctt gtcaatacca cgaagcaaaa gacctacatt
     2821 atcaccagcc tcacctctgt caaggatctt acggaacatc tcaactccgg taacaactgt
     2881 ttttttaggt tcttcaccaa gaccaataat ttgtacttct tcacctgttt taacaactcc
     2941 ggtctcaata cggcctgtag ccactgtacc acgtcctgta attgagaaga cgtcctcgac
     3001 aggcataagg aaaggtttgt cgatatctct cggaggtata ggaatatatt cgtcaacagc
     3061 atccatcagc tcaacaacct tctcttccca tttaggatca ccgttaagag ctccaagagc
     3121 agaaccgcga ataacaggag cgttatcacc gtcatattta tagaatgaaa gaagctcacg
     3181 aacttccatc tcaacgagat ctatcatttc aggatcgtca acaaggtcaa ccttgtttag
     3241 aaaaacgacg attctcggaa cgttaacctg gcgtgcaaga agaatatgct cacgtgtttg
     3301 aggcattgga ccatcagttg ctgctactac cagaatagca ccatccatct gtgctgcacc
     3361 tgtaaccatg tttttaacgt agtctgcgtg acccggacag tcaacgtgtg catagtggcg
     3421 tttttctgtc tgatattcaa tatgagaagt attaatggtt ataccacgtt ccttttcctc
     3481 aggagcatta tctatagagt cgaatgaacg aacctctgaa agtcccttgc gagctaatac
     3541 cttggtgata gctgcagtaa gagtagtttt accatggtca acgtgaccaa ta
//
LOCUS       DAOTQL010000149         3591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_20107_length_3591_cov_4.352658, whole genome shotgun sequence.
ACCESSION   DAOTQL010000149 DAOTQL010000000
VERSION     DAOTQL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20107_length_3591_cov_4.352658"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1153)
                     /locus_tag="P5293_07685"
     CDS             complement(155..1153)
                     /locus_tag="P5293_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRR49814.1"
                     /translation="MSNSYLQSIKERIFLIKSEEEFDDLALEIFDYQSVHCPIYSQYI
                     DLLGIDRQSIDDIEKIPFLPIEFFKSTKVYSVAEDPQKIFTSSATSGMVPSKHYVADL
                     SIYEESLLKSFTLEYGDPSQYTFLALLPSYLEREGSSLIYMVDRLMGLSGKSANGFYL
                     YNHSELFETLGKLRDKDEKSILFGVSFALLDFANEFTCYFPSLIIIETGGMKGRGREL
                     PREIIHQRLQNSFGVEKVHSEYGMAELLSQAYSHGNGLFNTPPWMKILIRDFNNPFKL
                     VQPEEVGGINVIDLANIGSCSFIETQDMGIKTAEDLFKITGRIKNSEIRGCNLLLA"
     gene            complement(1104..>2066)
                     /locus_tag="P5293_07690"
     CDS             complement(1104..>2066)
                     /locus_tag="P5293_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRR49815.1"
                     /translation="DFEKKIELMSPIITTEDVTNFSGVPSWNLVLMNKVLEVSGKNNL
                     LEVWPNLELFMHGGISFDSYRDIYKRLIPSDKMNYRENYNASEGYFGFQDDPKDESML
                     LLVDNGIFYEFIPMERFEQALNGLFKDFDTIDNVKKDTNYAVVLTTNGGLWRYLLGDT
                     VMFSSLKPHKFLITGRTQLFINAFGEELMINNAEKALAKTCSETGVEVTNYTVAPIFM
                     IRGGKGSHQWIIEFAKPPDNLDNFADLLDKNLCLFNSDYEAKRKNNATMVRLTLSPVS
                     NGTFYKWMKSKGKLGGQNKVPRLSNKRDYVEQLLAIDKGENISD"
     assembly_gap    2067..2166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2167..2353)
                     /locus_tag="P5293_07695"
     CDS             complement(<2167..2353)
                     /locus_tag="P5293_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49816.1"
                     /translation="MSLTTFIFRQISKIRLSQLDKTRINPWKSQTECFKNLIASGRDT
                     LFGKEHDFEKINNVEEFQ"
     gene            complement(2360..3019)
                     /gene="recO"
                     /locus_tag="P5293_07700"
     CDS             complement(2360..3019)
                     /gene="recO"
                     /locus_tag="P5293_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRR49817.1"
                     /translation="MPDKLQIIVLHTLKYKDSSLILQGYSNLNGRQSYIVRKRKPGNA
                     FISQLQALDIIDAEIVPGGKGSMSTIKEFVPAFTFQDIRYNLNKCSIAMFISELIYRI
                     VREVEPNRVMYDFIRSSILTLETLKNGYSNFHTWFIVEMCKQSGYDPLLSIGDFSGRS
                     YEILAKLLNIDPAELNTVKLNGKERYIFIKDMLSYLSQHIGFPIEVKSLEVLHQVLGQ
                     E"
     gene            complement(3019..>3591)
                     /gene="rdgB"
                     /locus_tag="P5293_07705"
     CDS             complement(3019..>3591)
                     /gene="rdgB"
                     /locus_tag="P5293_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRR49818.1"
                     /translation="VFATNNLHKVEEARSVLGKNFLLKTPRELNLFEDIPETADTLKG
                     NALIKAQFIWQRLHIACFADDTGLEVNALNGAPGVYSARYAGEQHDSKANMAKLLKEM
                     EGILDRRARFRTVIALIVDGKPFFFEGLLNGKIAEHPSGTGGFGYDPVFIPDGYDRTL
                     AELSPEEKNLISHRGNSLRKLSAFLNQYKK"
BASE COUNT         1042 a          656 c          628 g         1165 t
ORIGIN      
        1 caaaggcatg tatctttcaa agtatgatat tgaacaaaat ttgacatata ttctccattt
       61 gtcagatatc cttaagaaca gaggtgaaga aaagctttct gccgagatgg aaaagaatat
      121 cgaggcttac cttaagacaa tagaaggtta ggaatcatgc aagtaaaaga ttgcaacctc
      181 ttatttctga atttttaatc cggccggtga ttttaaaaag atcctcggcc gttttaattc
      241 ccatgtcctg agtctcaata aaggagcagg aacctatatt ggcgagatct ataacattta
      301 ttcctccgac ttcttcgggt tgtacaagct tgaaaggatt attgaagtct cgtattagta
      361 ttttcatcca tggtggggta ttgaacaatc cgttgccgtg agagtaggct tgagagagaa
      421 gttctgccat cccgtattca gaatggactt tttcaactcc aaatgaattt tgcaatcttt
      481 gatggattat ctctcttgga agttccctgc ctcgtccttt cataccacct gtttcaatga
      541 taattaacga ggggaagtag caagtgaact cgttggcaaa atcaagcaat gcaaagctta
      601 ccccaaataa tatggacttt tcatctttat ctctaagttt accaagtgtt tcaaataatt
      661 cggagtgatt gtataggtag aacccatttg cacttttacc agatagcccc attagcctgt
      721 caaccatata aatcagggaa gatccttctc tttcaagata tgagggtaga agtgcaagaa
      781 aagtgtattg tgagggatca ccatactcaa gtgtaaaaga tttcaaaaga ctttcttcat
      841 aaatagacaa gtcagcaaca tagtgttttg aggggaccat accagaagtg gcactgctgg
      901 taaaaatttt ttgtggatct tcggcaactg aatagacttt ggtactttta aaaaactcaa
      961 taggaagaaa tggaattttt tcaatatcgt ctattgattg cctgtcaatg cctaaaagat
     1021 cgatatattg actgtaaatt ggacagtgaa cagactggta gtcaaaaatt tccaatgcca
     1081 ggtcatcgaa ctcttcttcg gacttaatca gaaatattct ctcctttatc gattgcaagt
     1141 aactgttcga cataatctct tttgttggat agtcttggaa ctttattctg gccaccaagc
     1201 tttcctttac ttttcatcca cttgtaaaaa gttccattac ttacagggga tagagttagt
     1261 cttaccatag tggcgttatt ttttctttta gcttcataat cagaattgaa caggcaaaga
     1321 tttttgtcta gaagatcagc aaaattatcc aggttgtcag ggggtttagc aaattctatt
     1381 atccattggt gagagccttt gcctcctctt atcatgaaaa ttggcgcaac tgtgtagttt
     1441 gtaacctcta ctcctgtttc agaacaagtt tttgcaagag ctttttcagc attattgatc
     1501 atcagctctt caccaaatgc attaataaaa agttgagttc ggccggttat taaaaatttg
     1561 tgtggtttca gacttgaaaa catgacagta tctccaagca agtaacgcca tagtcctcca
     1621 tttgtagtta gaactactgc gtagtttgta tcctttttta cattgtcgat ggtgtcaaaa
     1681 tctttaaaca gcccatttaa tgcctgctca aatctctcca ttgggataaa ctcgtaaaat
     1741 attccgttat caaccaataa tagcattgat tcgtcttttg gatcatcctg aaatccaaag
     1801 tatccttcag atgcattgta attttcacga taattcatct tgtcagaagg gatcagcctc
     1861 ttatagatgt ccctgtatga gtcgaagctt attcctccat gcataaagag ttcaagatta
     1921 ggccacactt caagtaaatt attcttgcct gacacttcaa gaaccttatt cataagtaca
     1981 aggttccaag aaggaacacc tgagaaattt gtcacatctt ctgttgtaat aataggactc
     2041 atcaactcaa tctttttttc aaagtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnntttg aaattcttca acattgttta tcttttcgaa atcatgttct ttaccgaata
     2221 atgtatctct acctgatgct attaagtttt taaaacattc tgtctgactt ttccatggat
     2281 taattcgtgt cttatcaagc tgagaaagcc tgattttaga tatttgtctg aatataaatg
     2341 ttgttaatga catgtgtttt tattcttgtc ctaatacttg atgtaaaacc tcaagtgact
     2401 tgacctcaat ggggaaacca atgtgttggc ttaaatagga aagcatatcc ttaataaaaa
     2461 tatatctttc cttaccgttc aacttgacag tatttaattc tgcaggatca atattcaaaa
     2521 gttttgccag gatttcataa cttcttcccg agaaatcgcc aattgagaga agaggatcat
     2581 atccactttg tttgcacatt tcaacaataa accatgtatg gaagtttgaa tatccgtttt
     2641 ttaaagtttc gagtgtcaat attgaactgc gaataaaatc gtacatcaca cgatttggct
     2701 caacttctct aacaatcctg tagatcagtt cactgataaa cattgcgatg gaacatttgt
     2761 tgagattgta tctgatatct tgaaaagtaa atgcaggaac aaattcttta attgtagaca
     2821 tagacccttt tcctccaggt acaatctctg catcgattat atcaagtgct tgaagttgag
     2881 agataaaagc atttcccggt tttctttttc ggacgatata actttgcctg ccgttgagat
     2941 tggaataacc ctgcaatatc agtgatgagt ccttgtattt aagagtgtga agaactatta
     3001 tttgcagttt gtcaggcatt attttttgta ttgatttaga aaagcagata attttcttaa
     3061 agagtttcct ctgtgggata tcaaattttt ttcttccgga gacaattctg ccagagtcct
     3121 atcatatccg tcagggataa agacagggtc ataaccaaac cctcccgttc cagatggatg
     3181 ttctgcaatt ttaccattaa gtaatccttc aaaaaagaaa ggttttccgt ctacaataag
     3241 tgcaattaca gttctgaatc ttgctcttct gtcaagtatg ccctccatct ccttaagtaa
     3301 ttttgccata tttgctttgg aatcatgctg ttcaccggca taacgtgcag agtaaactcc
     3361 gggagctcca tttaaggcat tgacttcaag ccctgtatca tcggcaaaac atgcaatatg
     3421 gagccgttgc catataaatt gtgcctttat cagtgcattt cctttgagtg tgtcggctgt
     3481 ctcgggaata tcttcaaaaa ggttgagttc tcgaggagtt ttaagaagaa aattcttacc
     3541 caaaacagac cgggcttctt ctaccttgtg aagattattg gttgcaaata c
//
LOCUS       DAOTQL010000150         3489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_20864_length_3489_cov_3.689575, whole genome shotgun sequence.
ACCESSION   DAOTQL010000150 DAOTQL010000000
VERSION     DAOTQL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20864_length_3489_cov_3.689575"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="P5293_07710"
     CDS             complement(<1..844)
                     /locus_tag="P5293_07710"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRR49819.1"
                     /translation="MIRKAFSIFGLICCLVISLHGQENEKLEKYIDQYIAMTPPSTDS
                     LNAACDYLINISQDTAIQAHIAGHLFNKFYSSPIMGMESVAIHIAKNYFLNKKLNWRG
                     DDGMLFLRLFVEFNENSLIGMKAPELILPDTSGNLHSLRNLDAEYTIIYFYDTDCSLC
                     KKELPKIIEVSNKFKGYDLHIYAVYTQSDVNNLKQFIKEEFPKSIDNWTFVWDPDFKS
                     GFHKLYNVLKTPQIFLLDRDKKIIGRNLDHTSLDQLLSSYTESRTNLSKNIRAFLSDY
                     LKVVK"
     gene            complement(845..1330)
                     /gene="coaD"
                     /locus_tag="P5293_07715"
     CDS             complement(845..1330)
                     /gene="coaD"
                     /locus_tag="P5293_07715"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRR49820.1"
                     /translation="MNIALFPGSFDPITLGHIDILKSALKLFDKVVVAVGYNTQKTGL
                     FTVEERVKFIENGVRSLNLNNNIEIASYSGLTIDFCKRANIKFIIRGLRTTTDFEMER
                     VVAQANLSMAPDILTVFIPSRHEYSFISSTVVRDVIINGGDATPFLPDGVDISEYLNK
                     V"
     gene            complement(1333..2157)
                     /gene="dapF"
                     /locus_tag="P5293_07720"
     CDS             complement(1333..2157)
                     /gene="dapF"
                     /locus_tag="P5293_07720"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRR49821.1"
                     /translation="MIIKAHKYQGAGNDFIIIDNQNNNIELSAEQIRQLCHRRFGIGA
                     DGLMLLEKSEKYDFSMKFYNSDGIEGPMCGNGGRCLVAFAYHSGIKNYHFEASDGEHR
                     GTVLVGTPSKCIVELEMIDVHEVKEYSPKSFFLNTGAPHLVIFVEKLKDYDVLEQGRF
                     WRHHPSFPGGTNVNFVQGNWGRCAIPEGVDLFVRTFERGVEDETYACGTGVTASAIAF
                     HKLFQRNLYPSGTVSTRVQTIGDILDVKFDYNGEDIYSNIKLKGPATYVFTCEIEI"
     gene            complement(2147..>3489)
                     /locus_tag="P5293_07725"
     CDS             complement(2147..>3489)
                     /locus_tag="P5293_07725"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRR49822.1"
                     /translation="FYYIPPTVWGWKEWRIKSLKKYVDRIFIIFPFEVNYYKKKGIKV
                     FYFGNPLLDSIAGHKCNYESFEEFTKRCNLDQRPIITLLPGSRESEIKYLLPVFSKLE
                     NEFHDYQLIVAGAPSIDEKIYLPYISGTNIKLLFGETYSLLKHSKATALCSGTASLEA
                     AILDTPQVVCYRANPISVAIVRPFVKVKYLSLVNLIFNQPVVKELIQKDCTVENLACE
                     LRKILSEKESIKIKNKYAKLRKILGDASTSRRTAEAMLSEIQSLSYEHLYYKYYKSPI
                     GMLKLIADEDSLVAIRYIREDTEEQCLEKSNPKGNLILEEATRQLEEYFAEKRKIFDL
                     PLKPTGTPFQQKVWKELSNIPYGQVKTYGEIASIVETKDASRAVGNACRLNPLPIVIP
                     CHRVIGANNKLTGFNMGIDKKLFLLTLEKAYESSEVNLFNSNSNFNSNSIDNDN"
BASE COUNT         1062 a          645 c          600 g         1182 t
ORIGIN      
        1 gttttacgac tttaaggtaa tcactcagaa atgcacgtat gttttttgac aagtttgttc
       61 tgctctcagt atatgaactt aataattggt ctaatgatgt gtggtcaaga ttgcgaccaa
      121 taattttttt atctctgtca agaagaaata tttgaggagt tttaagtaca ttataaagtt
      181 tatgaaatcc acttttaaaa tccgggtccc acacaaatgt ccaattatca attgattttg
      241 gaaattcctc cttgatgaat tgcttaagat tatttacatc gctttgtgta taaacagcat
      301 atatatgcaa atcgtagcct ttgaatttat ttgaaacttc aattattttc ggaagttcct
      361 ttttacacag actgcaatcc gtatcataaa aatagatgat agtgtattca gcatccaaat
      421 ttcttaaaga atgcaaattg ccggaagtat caggaagtat aagttcagga gctttcatcc
      481 ctattaggga attctcgtta aattcgacga agagtctgag gaaaagcatt ccatcatctc
      541 ccctccagtt gagttttttg tttaaaaaat agtttttggc aatatgaatg gcgacacttt
      601 ccattcccat gattggggag gaataaaatt tattaaaaag atgtccggca atatgagcct
      661 gaatagcagt gtcttgactt atattgatta aataatcgca ggcggcattt aatgaatctg
      721 tcgaaggagg agtcattgca atatactggt caatatattt ctccagcttc tcattttcct
      781 gaccatggag actgatgaca aggcaacaaa ttaaaccaaa tattgaaaat gccttgcgaa
      841 tcattcagac tttatttaaa tattcagata tgtcaactcc atcaggaaga aaaggggttg
      901 catcacctcc gtttattata acatctctta ctacagtaga agagataaat gagtattcat
      961 gacgtgatgg tataaaaaca gtaagaatat ccggggccat acttagattg gcttgagcaa
     1021 caaccctttc catctcaaaa tcagttgtgg ttcttagtcc tcgtattatg aacttaatat
     1081 ttgccctttt acaaaagtct attgtcaatc cagaatagga ggcaatttca atattattat
     1141 ttagattaag tgacctgacg ccattttcta taaatttcac tctctcttca acagtaaaaa
     1201 gtcctgtttt ctgggtatta tatcctacgg cgacaacaac tttgtcaaaa agttttagcg
     1261 cagattttaa gatatctata tgtcctaatg taatcggatc aaaagatccg ggaaataatg
     1321 caatattcat gatcatattt caatttcaca agtaaagaca taagtggcag ggcctttcag
     1381 ctttatgttg gaataaatgt cttcgccatt atagtcaaat ttaacatcaa gaatatcacc
     1441 aatagtttgt actcttgtgg aaacagttcc tgaaggatag agattccgct gaaataattt
     1501 atgaaatgca attgctgaag cagtaacacc tgtgccacat gcatatgttt catcctcaac
     1561 acctctttca aaagtcctga cgaaaaggtc gacaccttca ggaatagcac atcttcccca
     1621 gttaccctgt acgaaattaa catttgtacc tccagggaaa cttggatgat gtctccagaa
     1681 cctgccctgt tccagaacat catagtcttt taatttttcg acaaaaataa ccaggtgcgg
     1741 agctccggta ttaagaaaaa acgatttcgg agagtactcc ttaacttcat gaacatctat
     1801 catttcaagc tcaactatac atttcgaagg agtgcctacg agcactgtcc ctctatgttc
     1861 tccatcactt gcctcaaaat gataattttt tattccagaa tgatatgcaa aagcaaccag
     1921 acaccttcct ccatttccac acatagggcc ttctatcccg tcagaattgt agaatttcat
     1981 cgagaaatca tatttttcac ttttttcgag tagcatcaga ccatctgccc caattccgaa
     2041 tcttctgtga cataattgtc tgatttgttc tgcagatagt tcaatattat tattttggtt
     2101 atctataata atgaagtcgt tacctgctcc ctggtactta tgcgccttaa ttatcattat
     2161 caatactatt tgaattaaag ttcgagtttg agttaaaaag atttacttca gagctttcat
     2221 aagctttttc gagagtaaga agaaatagtt ttttatctat tcccatgttg aaacctgtta
     2281 gtttgttatt tgcgcctata accctgtgac aaggaattac tatcggaaga ggattcagcc
     2341 tgcaagcatt acctacggct ctgctggcat ctttagtttc tacaattgag gctatttcac
     2401 cgtaagtctt aacttggcca tagggaatat tagacagttc tttccagact ttctgttgaa
     2461 aaggagtacc ggttggtttt aaaggaaggt caaaaatttt acgcttttcc gcaaaatatt
     2521 cttccagttg ccttgtggct tcttcgagta ttaaattccc tttagggttg gatttctcaa
     2581 ggcactgttc ttccgtgtct tctctgatat atcttattgc aacgagagaa tcttcgtctg
     2641 ctatgagttt aagcatgcca ataggagatt tataatattt ataatataaa tgctcataag
     2701 ataaagattg aatctcactc aacatagctt ctgctgttcg tcttgaagta ctggcgtctc
     2761 ccaatatttt tctcaatttg gcatatttgt ttttgatttt tatgctctct ttttctgaaa
     2821 gaatcttacg gagttcacat gctaaatttt caacagtaca gtccttttga ataagctctt
     2881 tgactacagg ctgattgaaa ataagattta cgagacttaa atattttacc tttacaaatg
     2941 gacggactat tgctactgag attgggtttg ctctgtaaca tactacctga ggcgtatcga
     3001 gaattgctgc ttcaagactt gctgtaccgg aacataatgc agtagccttg gaatgcttta
     3061 gtaacgagta agtctcacca aacaggagtt ttatatttgt acctgatatg tatggaaggt
     3121 atattttctc atcaattgat ggtgctccgg cgacaattag ttgataatcg tggaattcgt
     3181 tttcaagttt ggaaaaaaca ggcaaaaggt attttatctc actttctcta ctgccaggaa
     3241 gcaaagtgat aatgggtctt tgatcaagat tgcatctttt ggtaaattct tcgaaactct
     3301 cataattgca tttatgacca gcaatactgt cgagtagggg atttccaaaa tagaaaacct
     3361 taattccttt tttcttatag taattaactt caaatgggaa aattataaag attctgtcta
     3421 catatttctt tagactttta attctccatt ctttccatcc ccagacagta ggaggtatgt
     3481 agtaaaata
//
LOCUS       DAOTQL010000151         3446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_21164_length_3446_cov_4.263639, whole genome shotgun sequence.
ACCESSION   DAOTQL010000151 DAOTQL010000000
VERSION     DAOTQL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21164_length_3446_cov_4.263639"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="P5293_07730"
     CDS             complement(<1..1000)
                     /locus_tag="P5293_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRR49823.1"
                     /translation="MEAKKIIMLPENEMPRQWYNIVPDMLVQPKPMLNPKTREPLLPE
                     EMEGLFAKALVEQEFSQKRWHDIPDEVLELYKIYRPTPLVRANLLEKALDTPAKIYFK
                     NESVSPVGSHKLNSALPQAYYNHIQGIKAITTETGAGQWGSAMSLACRHFGLKLEVFM
                     VKNSYNAKPYRKVVMKTYGADIYASPSDITQYGKQILADDPNNEGTLGIAISEAVERA
                     VTRPNTRYTLGSVLSHVIMHQTIIGLEAEKQMEIAGDYPDKVIACFGGGSNFSGISFP
                     FLRHKLTEGRSIKLIAAEPAACPKLTTGKLEYDFGDAAGLTPLIPMYTLGHDFQPEQ"
     gene            complement(1208..3442)
                     /locus_tag="P5293_07735"
     CDS             complement(1208..3442)
                     /locus_tag="P5293_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRR49824.1"
                     /translation="MSTIFFACLAFSLNDVYAASNFNNPIVFGNNRITLITPTLFRLE
                     YALDGKFLDSTTMFAYTRDTLLSDFKVTELGDNKFLIETKALRMVYENDGFPFGIHNF
                     TVFFKLNGEEKKFTVRNIHKNNLGGAISTLDRVNQKVPVEDGLLSRDGWYIINDTGKE
                     ILSDGWIASRDKSHVQDLYCFVYGDDYKAALMDLGAISGHVPMTRKYIHGVWYCRYWP
                     YTADEYEKLVQEYRVNDFPLDNLVFDMDWHTVDAKVGTGHASSRGWTGYTWNKTLIPD
                     PKGLIDNLKLDHINVCLNEHPHDGIRPHESVYSDFMRAMGAEPNGQTILFDASDKKYM
                     DNFLKYAHKESWDMGVAFWWLDWQQDYLYPYVRGTNMNHLTWLNKLYYEESQRNGLRG
                     AGYSRWAGWGDHRYPVQFSGDLHANWNTLAFEVELTATSGNAGCYFWAHDIGGFYGGD
                     NPEMMTRWTQFGALSSSLRVHSQKAPNLDRRPWLWGEQAKNAMRIAYHFRSQIMPYLY
                     TSIWQTHTTMIPLNRAMYIDYGQFEESYVNPQQFMFGDLLLAAPITSPGEGENFIASQ
                     TIWFPDGNIWYDFFDGTMYKGGTKVTIKKDINSFPLFVKGGWMLPMQPYTERPASSPL
                     TCLIVRAYPGEDGSDNTYILYEDDGVSMEYTVGKFATTDLRYVRNGNQIKLYVSPVNG
                     TYKGQPQKRSYIFELPAMNKIKSLKVNNKKYSEFNMKNGMMIVEVPALSIGASVNVEI
                     TVFE"
BASE COUNT         1061 a          756 c          549 g         1080 t
ORIGIN      
        1 tctgttcagg ctgaaaatca tgccccagag tatacattgg gatcaaagga gttaatcccg
       61 ctgcatctcc aaaatcatac tccaacttac ctgtagtgag ctttggacaa gcggcaggct
      121 ctgctgcaat taatttaata ctacgccctt ctgtcaactt gtgtctcaaa aacggaaacg
      181 aaatccctga aaaatttgac ccacctccaa aacatgcaat gactttgtca ggatagtctc
      241 ctgctatttc catctgtttc tcagcttcaa gaccaataat agtttgatgc ataatcacat
      301 gactcaaaac actgccaaga gtatatctgg tatttggtct ggtaactgct ctttcaacag
      361 cctcagatat ggcaattccc aaagtgcctt cattattagg gtcatcagct aatatttgct
      421 ttccatattg tgtaatatct gatggtgagg cgtaaatgtc agcgccataa gtcttcatta
      481 caacctttcg ataaggcttt gcattataac tattctttac cataaatacc tcaagcttaa
      541 ggccaaaatg tctgcaggca agactcattg ctgatcccca ctgaccagct cccgtctctg
      601 ttgtaatagc tttaattccc tgaatatgat tgtaatatgc ctgtggcaat gctgaattta
      661 gtttatgaga cccaaccgga ctaacacttt cgttcttgaa ataaattttc gctggtgtat
      721 ccaaagcttt ttcgagtaaa tttgccctga caagaggcgt aggtctataa attttataca
      781 gctctaaaac ctcatcaggt atatcatgcc atcgtttctg tgaaaactcc tgttcaacca
      841 gagcctttgc aaaaaggccc tccatctctt caggaagaag aggctctctt gttttaggat
      901 tcaacatcgg tttaggttgt accaacatgt caggaacaat gttataccat tgtcgaggca
      961 tttcgttttc cggtagcata ataatttttt ttgcttccat cgataataag tttaaaatgt
     1021 gttaaaaact aaaataaaaa agcccgacgc gtgagcatcg ggcttgtata catcaataaa
     1081 ttataaaaac aggttataca cgaaaagcgc agctcacagc tctaaaagtg ctgtgccacc
     1141 accaactttg tgtaaaaatc gctttcattt gtgcaaaagt aaatatttat ccaaaatgaa
     1201 caaaaaatta ttcaaaaact gttatctcaa cattaactga cgctccgata gaaagagctg
     1261 gtacctcaac tatcatcata ccatttttca tgttaaattc cgaatacttt ttattattta
     1321 ctttcaatga cttaatcttg ttcatagcag gcaattcaaa tatatatgac cttttttgag
     1381 gttgaccttt ataagtacca tttacgggtg aaacatacaa tttaatttga ttcccgtttc
     1441 taacatatct aagatcagtt gtagcaaatt tcccaactgt gtattccata ctcactccgt
     1501 cgtcttcata aagaatataa gtattatctg atccatcctc acccgggtaa gctctaacta
     1561 taagacaagt caaaggtgaa gaagcaggtc gttctgtata aggctgcatt ggcaacatcc
     1621 atccaccttt aacaaataaa ggaaaagaat taatgtcttt cttaatagtc acttttgtac
     1681 ctcccttata cattgtacca tcaaagaaat cgtaccatat gtttccatct ggaaaccaga
     1741 ttgtttgaga agcaataaaa ttctctcctt ctcctggtga agttattggt gcagcgagta
     1801 aaagatctcc aaacataaat tgctgaggat ttacgtatga ttcctcaaat tggccatagt
     1861 caatatacat tgccctgttt aaaggaatca tcgttgtatg tgtctgccaa atacttgtat
     1921 ataaatatgg cattatctgc gatcgaaaat gatatgcaat ccgcatagca ttcttggcct
     1981 gttctcccca caaccaaggc cttctatcta aattcggagc tttttgagag tgaactctta
     2041 gcgaagagct aagtgctcca aattgcgtcc atctggtcat catttcagga ttatcaccac
     2101 cgtaaaaacc tccaatatca tgtgcccaaa agtaacatcc tgcatttccg cttgttgcag
     2161 tcaattcaac ttcaaaagca agggtgttcc aattagcatg caaatctcct gaaaattgga
     2221 ccgggtacct gtgatctccc catcctgccc atcgactgta tcctgcccct cttaatccat
     2281 ttctttgtga ttcttcgtaa taaagtttat taagccaggt aagatggttc atattagttc
     2341 ctctcacata aggataaaga tagtcctgct gccagtcaag ccaccaaaat gctactccca
     2401 tatcccagct ttccttatga gcatatttta aaaaattgtc catatatttt ttgtcggaag
     2461 catcaaacaa tattgtctgt ccgtttggtt cagcccccat tgctctcata aaatcggaat
     2521 aaacactttc atgaggacga attccatcat gaggatgctc attcaaacac acgttgatat
     2581 ggtcaagttt aagattgtct atcaaaccct taggatccgg aataagtgtt ttattccatg
     2641 tatagcctgt ccatcctcgg ctgcttgcat gtcctgttcc aaccttggcg tcaactgtat
     2701 gccaatccat atcaaaaacg agattatcaa gaggaaaatc atttactcta tactcctgta
     2761 ctaacttctc gtattcatcg gcagtataag gccaatatct acaataccag acgccatgta
     2821 tatattttct tgtcatgggt acatgtccgg aaattgcacc aagatccatt aaagcagcct
     2881 tataatcatc accataaacg aaacagtaaa ggtcctgtac gtgagatttg tctcttgaag
     2941 caatccaccc atcagataat atctctttcc ctgtatcgtt tatgatatac caaccatctc
     3001 tgcttaacaa accatcttca acaggcactt tttggttcac tctgtcaagt gtagatatcg
     3061 ctccccccaa attattctta tgaatatttc taacagtaaa ttttttttcc tctccattca
     3121 acttgaaaaa gactgtaaag ttatgtatac caaaaggaaa tccatcgttt tcatatacca
     3181 tcctaagagc tttcgtctct atcagaaatt tattgtctcc caattcagta actttaaaat
     3241 ctgaaagaag tgtatctctt gtataagcga acatggtcgt agaatcaaga aatttaccat
     3301 ccaatgcgta ttcaagtcgg aacaatgtag gagtaattaa agtaattcgg ttatttccaa
     3361 aaacaatagg attgttaaaa tttgatgctg catatacatc atttaaacta aatgccaggc
     3421 aagcaaaaaa tatagtactc aacttt
//
LOCUS       DAOTQL010000152         3443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_21184_length_3443_cov_4.846812, whole genome shotgun sequence.
ACCESSION   DAOTQL010000152 DAOTQL010000000
VERSION     DAOTQL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21184_length_3443_cov_4.846812"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="P5293_07740"
     CDS             <1..663
                     /locus_tag="P5293_07740"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRR49825.1"
                     /translation="LVVPFASGVAEETGRRQSEEFRNLGCKSADYIYCAKDSIDSPEN
                     LAKLDGVTAIFFSGGDQVILSSYLEGTKFLEKIREIYKNGGVVSGTSAGAAIMSRIML
                     TGTSRSDTSDMPEFNYIKAGDVVTAKGFGFLSNVIVDQHFITRKRQLRLINVLLDNPG
                     YRGIGIDEESAIVVKPDNSMEVIGNNNVMLFEPYKQTNENGKKTKSFKLTILYPGDIY
                     KF"
     gene            675..2278
                     /locus_tag="P5293_07745"
                     /pseudo
     CDS             675..2278
                     /locus_tag="P5293_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137759.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            2315..>3443
                     /locus_tag="P5293_07750"
     CDS             2315..>3443
                     /locus_tag="P5293_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HRR49826.1"
                     /translation="MKKNIFISILLIVIFASCKTLIARDMILTPAEKSSFREVSKYAD
                     IVTFIHDLDNSSSLLTVETIGHSVEGRNVYALKFSTTTFGSDKSKIRVLIHAQQHGNE
                     QSGKEGALLIARELLKPENRVIFDHIDLAIVPQVNPDGSEKNIRRNGNNVDLNRNHLI
                     MTEPEVLALHKLFDKYLFEVTMDVHEYFPYSKEWERLGYRKHSDILLGFNTHPLIPES
                     IREYQLKKCWPYLSSQLESKGVSNSIYSPGGPPEIDYVRFSTFDINDGRQSFGIQNTF
                     SFIQEGLNGEDGYVENLAHRAYSQSQGIYFLLKYVSEHYIEIKSTVETLRRTQSLTES
                     IIPLRMEHMPDGSKFNMPVYSYKTGKDSTLVVNDFRPKVVPT"
BASE COUNT         1047 a          562 c          714 g         1120 t
ORIGIN      
        1 ctcgtagtac cctttgccag tggagtagca gaagagactg gcagaagaca gagtgaagaa
       61 ttcagaaatc ttggatgcaa aagtgctgat tacatttatt gtgcaaaaga tagtatagat
      121 tctcctgaaa atcttgcaaa acttgatgga gtaactgcca tatttttctc tggtggtgac
      181 caggtgatat taagtagcta tcttgagggc actaaatttt tggaaaaaat aagagaaata
      241 tataaaaatg ggggagttgt aagtggaaca agtgctggcg cggctattat gagtagaata
      301 atgcttaccg gaacaagtcg gagcgataca tctgatatgc ctgagtttaa ttatataaaa
      361 gcgggtgatg tcgttaccgc caagggattt gggttcctta gcaatgttat tgtagatcag
      421 cattttataa ctcgaaaaag acagcttcgt cttataaatg ttttgctcga caacccaggt
      481 tatagaggga taggaattga tgaagagtcg gcaattgtag ttaaaccaga caattccatg
      541 gaggtaattg ggaacaataa tgtaatgctt tttgaaccat ataagcaaac taatgaaaat
      601 gggaagaaga caaaaagctt taaattgaca atattatacc ctggtgatat ttataaattt
      661 taaaaattgg agtaatggcg aaacttagca agatgcctca cacgtttgtg attatcggat
      721 gtctcatttt gttatgtgga atagctacat ggtttgttcc ggcaggagag ttcaagtatg
      781 aaaccaaaga tataaatgga gttcaaagag aagtaattgt taacgggtca tatcacaagg
      841 tcgaacaatc tcctcagaca tggcagattt ttggctcact tctcgaaggg tttaaaaaac
      901 agtctggtat aattgctttt gttttgataa ttggaggagc atttcagata ttaacagcag
      961 tcgcgcaatt gattttggaa ttctatcttt tcttgaaaaa tccggccaac ttgagaaaaa
     1021 atggcttttc agaaaaattg gggtcaacaa tctggttatt attgggtcaa tgctcatgtt
     1081 tagtcttttc ggagccgttt ttgggatgag tgaggagaca cttgctttta tcaccatttt
     1141 tgtacctctt gccatatcaa tgggctatga ttcaattacc ggtttgctta tggtatatgt
     1201 ggctgctcac atagggtttt caggtgctat aattaatccc tttaccattg gtatagctca
     1261 gggactttct gggcttcaac ttttttcagg tattgaatac agaatatttg tctggatatt
     1321 gttgactact ttaataatat gtttcaccct ttattatgct tcaagggtta aaaagaaccc
     1381 gaaaatatca cctatgtatg aactggatga gtactggagg cacagagaac gagaggctgt
     1441 tcatgttgat tttgcaaaga aaactcctgt atctgcctgg gtggtatttg tgatggttac
     1501 tgttgttctt gtcctgttat caataaatca tcctacaact acaattaaat taggaggttc
     1561 tgaatctaca tttgtatgtc tgccgattct tacattaatt tttgcaatta ctggattttt
     1621 tacccttaga aaatcctacc tgttttttat tttagatgtt ctggcgttta ccattgtttt
     1681 tcttgtgatt ggtgtcttgg gttacggatg gtatcttaca gagatatctg cactctttct
     1741 tgcgatgggc ataattgccg gtatagccat gggatcgagt gccaatgcaa tagttcatga
     1801 gtttattgct ggggctaaag atatacttac tgctgcattg attgttggtc ttgcaggtgg
     1861 tataatagag gtacttacaa agggtaagat catggataca attctctatt ctctttccac
     1921 aacaatggaa ggagcaggga agggagccac cgtctctata atgtatttta ttcaaacctg
     1981 cattaacatc tttattcctt caggatctgc aaaggcggct cttacaatgc caataatggc
     2041 tccattttct gatgtgattg gtctttcaag acaggctaca gttatggcct ttcaatttgg
     2101 tgacggcttc acaaacatga ttactcctac ttctgccgtt ttaattggag cactgggaat
     2161 agccagaatt ccgtatgagg tttggataaa atggttctgg aaaattttac tggcattcat
     2221 gattttgggt ctgatacttc taatgccaac agtatatatg caactaaatg gtttttagtt
     2281 tattataaac ataatttaat ttatattttt gtagatgaaa aaaaatatat ttatctctat
     2341 tttgctgatt gtaatatttg cttcttgtaa aacattgatt gccagggata tgattttaac
     2401 gccagctgaa aaatcttctt tcagggaggt gtcaaagtat gccgatattg tgacatttat
     2461 tcatgatctc gataatagtt catctcttct tactgtcgaa actattggtc attctgttga
     2521 aggaagaaat gtatatgcat taaagttttc tactactaca tttggttctg acaaatcaaa
     2581 aatcagagtt ttaattcacg ctcagcaaca tggcaacgag cagtctggca aagaaggtgc
     2641 actattgatt gccagagagc tccttaagcc ggaaaacagg gttatatttg atcatattga
     2701 cctggcaatt gttccacaag tcaatcctga cggatccgaa aagaatatac gtcgtaacgg
     2761 aaacaatgtc gatttaaaca gaaatcactt aataatgacg gaaccggaag tattggcact
     2821 tcataaactt ttcgacaaat acctgtttga agttacaatg gatgtacatg aatactttcc
     2881 atacagtaag gagtgggaaa gactgggata tcgtaaacat tccgatatct tacttggatt
     2941 caatacacat cctttgatac cagaatcaat tcgtgaatat cagctaaaaa aatgttggcc
     3001 ttatttatca tctcaactgg agtcaaaggg agtttccaat agtatttaca gtcctggtgg
     3061 tccaccagaa attgattatg tacgcttcag cacttttgat ataaatgatg gaagacaaag
     3121 ttttggtatt caaaacactt tttcatttat tcaggaaggg ctcaatggtg aggatggata
     3181 tgtggaaaat ttggcacaca gagcatattc tcaaagccag ggaatatatt tcttgctgaa
     3241 atatgtatca gagcactata ttgaaataaa gtcgaccgtt gagaccctaa gacgtacaca
     3301 aagtctaaca gaatcgataa tacctcttcg tatggagcat atgcctgatg ggagcaaatt
     3361 caacatgcct gtttattcat acaaaactgg caaagattca acactggtcg ttaatgattt
     3421 tcgtcctaaa gttgttccta cac
//
LOCUS       DAOTQL010000153         3408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_21456_length_3408_cov_4.517745, whole genome shotgun sequence.
ACCESSION   DAOTQL010000153 DAOTQL010000000
VERSION     DAOTQL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21456_length_3408_cov_4.517745"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="P5293_07755"
     CDS             <1..747
                     /locus_tag="P5293_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRR49827.1"
                     /translation="RSKSSILKEVKRMSSMVEFKGIISDVGGPTANMYGMYGKNLELC
                     KKCIKDSCIYPKICNNLNRDLSTLIELYHEIEKVKGVKKIFIGSGIRYDLILNETGYL
                     DETCKKYFREVVTRHTSGWFKIAPEHTEEHVLKAMGKPSFSSFIFLREEFSRITKEER
                     LNYQIIPYFMSSHPGCTICDMRTLSENPALRGIRLDQVQDFTPTPMTRSSVAFYAGID
                     PKTMKNIYVERDLINRKKQRSFFSRKSDKI"
     gene            809..1174
                     /locus_tag="P5293_07760"
     CDS             809..1174
                     /locus_tag="P5293_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49828.1"
                     /translation="MDKKTNTNKMNSAKRRAVISYANMSPELMAAFKEKYPRGYTDYM
                     GEIFKVDKPDGTFFYAISLEVSDAIYLVKIDVKIDDYEDAEQGLFGSGYAEDEVDEPD
                     EFPDDDASPAFSEEEDSDD"
     gene            1252..3027
                     /locus_tag="P5293_07765"
     CDS             1252..3027
                     /locus_tag="P5293_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRR49829.1"
                     /translation="MTFFLSHTIKIKDRIKEMPESNMLLFLALVVGFCSGLAAVFLKY
                     SIRFISQLLKGWFSGSSDSWLYLLYPGIGMLLSLLFVKYVVKDNISHGVTKVLQAIAK
                     HDSKIKKHNIWSSLVSSSFTIGFGGSVGAEAPIVYTGAAIGSNLAQRLGLSYKNMTIL
                     LGCGAAGAIAGIFKAPLAGILFTLEVLMFNVSMTSIVPLLVSTVTATTVSFLFLGESV
                     VFSNSIQMFSMHNIPFYIILGIVCGFFSLYFMRTALFIEDKIKLVKNPYTRWLITSLA
                     LGILILLFPPLYGEGYDSLTALLNNDVNSAIKTNIFGNVFNNQWAVPVFFLAVLVFKV
                     LAMSLTNSGGGVGGTFGPTLVVGGLLGFVFSRFINLVGGYGIPEANFALAGMAGLMAG
                     VMQAPMTAIFLIAEISGGYILLIPLIIVSVVSFATMRRFEKYSIYTKRIANEGDLLTH
                     DSDRAVLTLLKTSDLIETDFVPIEPHKHLRDLVDLISNCKRNIFPVVTKDGVLVGIIL
                     LDDIRHIMFDLSLYDSKQVLDVMIQPPAYVYEKEKMESVMRKFEETGAWNFPVIDLEG
                     HYKGFVSKSKIFFAYREQLQQVSHE"
     gene            3020..>3408
                     /locus_tag="P5293_07770"
     CDS             3020..>3408
                     /locus_tag="P5293_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HRR49830.1"
                     /translation="MNSLYINYITSSMSISAWQVEHCVQLLEESATVPFISRYRKERT
                     GSLNEVQVSEIKHYYNKFNELDKRKTAIIRSIEEQGKLTSDLKELILSTIDANRLEDL
                     YLPYKPKRKTKATIAKEKGLEPLAYKI"
BASE COUNT         1092 a          529 c          657 g         1130 t
ORIGIN      
        1 cgttctaaaa gttctatttt gaaagaagtg aaaagaatgt catctatggt agagttcaaa
       61 ggaataatct ctgacgtggg tggtccaact gccaatatgt atggaatgta tgggaaaaac
      121 cttgagcttt gtaagaaatg tattaaagac agttgtattt acccgaagat ctgtaacaat
      181 cttaatagag atctctctac attaattgag ctttatcacg aaattgaaaa agtgaaaggc
      241 gtaaaaaaaa tatttatagg aagtggcata aggtatgact tgattttgaa tgaaacaggt
      301 tatcttgatg aaacatgtaa aaaatatttc agagaggttg taacaagaca cacttccgga
      361 tggtttaaaa ttgctccaga acacactgaa gagcatgttc ttaaggcaat ggggaagcca
      421 tctttcagct cttttatatt tttaagagaa gaatttagta gaattacaaa agaagaaaga
      481 ttgaattatc aaataattcc ttattttatg tcaagccatc ctggctgcac aatttgcgat
      541 atgaggactc tatctgagaa ccctgcatta agaggaatca ggcttgatca agtccaggat
      601 tttacaccta cacctatgac aagatcatca gtggcatttt atgctggaat tgaccctaaa
      661 acaatgaaaa acatatatgt ggaaagagat ttgataaaca gaaaaaaaca aaggtcattt
      721 ttttcaagaa aaagtgataa aatttgactt aatataaaaa tttgcattat aattgcagcc
      781 caaacaacaa tgaactcact ttacggtaat ggataaaaag acaaacacta ataaaatgaa
      841 ttctgcaaaa cgaagagcag ttataagcta tgcaaatatg agtcctgagc ttatggcagc
      901 gtttaaggaa aaatatccaa gaggctatac cgattatatg ggtgaaatct tcaaagtgga
      961 caaacctgac ggtacttttt tttatgcaat ttcacttgaa gtttctgatg caatttatct
     1021 tgtcaaaatt gacgtgaaga ttgatgatta tgaagatgcg gagcaaggac tttttggttc
     1081 aggttatgct gaagatgaag ttgatgaacc tgatgaattt cctgacgatg atgcatctcc
     1141 tgctttctct gaagaggagg attcagacga ctaacagatt ttattatatt tacttataca
     1201 aggctgggta gttattcgcc cttttttttg tactttcgtg tctaaaatat aatgactttt
     1261 tttttatcac ataccattaa aatcaaggat cgaataaaag agatgcctga gagtaatatg
     1321 ttgctcttct tggcattagt tgtagggttc tgcagcggac tggctgcagt atttttaaaa
     1381 tattcaattc gttttataag ccaacttctc aaaggctggt tcagtggttc ttcagacagt
     1441 tggctctatt tgctatatcc aggcattggt atgcttcttt cacttttatt tgtaaaatat
     1501 gttgtaaagg ataatatcag tcacggtgtt acaaaagttt tgcaggctat tgccaaacat
     1561 gattcaaaaa ttaagaagca taatatttgg agctctcttg tatcaagttc atttactata
     1621 ggttttggtg gttcagtcgg tgcagaagct ccaattgttt atacaggagc agctataggt
     1681 tcaaatcttg ctcaaaggct gggtctttct tataaaaaca tgacaattct ccttggttgt
     1741 ggagctgctg gtgcaatagc cggcattttt aaagctccac ttgcaggaat tctctttact
     1801 cttgaggtat tgatgtttaa tgtatctatg acctcaatag tccctttact ggtttcaaca
     1861 gtcactgcca ctactgtatc atttctattt ctgggggaat cagttgtatt tagcaacagt
     1921 atccagatgt tcagcatgca caatatacct ttttatatta ttttgggtat agtatgtgga
     1981 tttttttctc tttattttat gagaacagcg ctttttattg aagataagat aaaattggta
     2041 aaaaatccat atacaaggtg gcttattaca tctcttgctc ttggaatatt gattttactc
     2101 tttcctccac tttatggaga gggctacgat tctcttactg ctctattaaa taatgatgta
     2161 aattctgcta taaaaaccaa tatttttggt aatgtattta ataatcaatg ggctgtacct
     2221 gttttttttc ttgctgtact tgttttcaaa gttttagcaa tgtctttaac taattcaggt
     2281 ggaggagtag gaggaacatt tggccctaca cttgtcgttg gaggattgct tggatttgtt
     2341 ttcagccgtt ttataaatct tgtcggagga tatggaattc cagaggctaa tttcgccctt
     2401 gccggcatgg caggacttat ggcaggagtg atgcaggctc cgatgactgc catatttctg
     2461 attgctgaga tttcgggggg gtacattttg ctgattcctc ttattattgt atcggttgtc
     2521 tcttttgcca ctatgaggag atttgaaaaa tattcaatat acaccaaaag aattgcaaat
     2581 gagggtgatt tattgactca cgacagtgat agggctgtac taaccctttt aaaaacttct
     2641 gaccttattg agactgattt tgtaccgatt gaacctcata aacatctaag agatcttgtg
     2701 gatttaattt ccaattgtaa aaggaatatt tttcctgttg ttacaaaaga tggagttctt
     2761 gtaggaataa tccttctcga tgatattcgt catattatgt tcgatctctc tctttatgat
     2821 tcaaaacaag ttttagacgt aatgatacag cctcctgcct atgtctatga aaaagaaaaa
     2881 atggagtctg tcatgaggaa atttgaggag actggtgcat ggaattttcc ggttattgat
     2941 cttgaaggac attataaagg atttgtctca aagtcaaaaa tattttttgc atatagagaa
     3001 caattgcaac aagtttctca tgaatagtct atatattaat tatattactt caagcatgtc
     3061 catatctgca tggcaggttg aacattgcgt acagctttta gaagaaagtg caactgtacc
     3121 ttttatttcc aggtacagaa aggaaagaac aggatcgtta aatgaagtac aagtatcaga
     3181 gattaaacat tattacaata agttcaatga actagataaa cgtaaaaccg caataatcag
     3241 aagtattgaa gagcaaggaa aattaacttc cgatctgaaa gagttgatac tatctactat
     3301 cgatgcaaac agacttgaag atttatatct tccttataag cccaaaagaa aaacaaaagc
     3361 tactattgct aaagaaaaag ggctggaacc tcttgcttac aaaatttt
//
LOCUS       DAOTQL010000154         3404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_21488_length_3404_cov_3.722305, whole genome shotgun sequence.
ACCESSION   DAOTQL010000154 DAOTQL010000000
VERSION     DAOTQL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21488_length_3404_cov_3.722305"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="P5293_07775"
     CDS             <1..1193
                     /locus_tag="P5293_07775"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRR49831.1"
                     /translation="FFGYNNPQPLQREQLTAGSGVIIHPDGYIVTNSHVVSGADEISV
                     TLENNQTYKAKLIGADEATDIALIKIDATGLPYLEFGDSDSLRLGEWVIAIGNPYNLT
                     NTITAGIVSAKRRSMSSYDSNFSDGKFKIESFIQTDAAVNPGNSGGALVNAKGELVGI
                     NTAIASRTGSFTGYSFAVPASIAKKVVNDFINFGEVKRAMLGVSMQEIDSEAAKRMNM
                     KEIKGVLVTDVMKGGAAEKSGIKPSDVILSINGQPVNSSPAVQEQISKYRPNDKINVE
                     ILRDGKPKLISVILQEENSKLLYEGSINAELQNTIGATLQKASKETLKKLSLRNGVEV
                     TSVGSGKFREAGIKPGFIITYINQVEVNEPSEVLAIIKKSGRSLLVEGVYPDGRVVYY
                     GMGL"
     gene            1244..2104
                     /locus_tag="P5293_07780"
     CDS             1244..2104
                     /locus_tag="P5293_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HRR49832.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREELITVEDEVELAQRIK
                     KGDQEALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKALSKFEQENERMPSPEELAE
                     ILDIPKEKIADTLRVSGRHVSVDAPFVDGEDNNLLDVLVNNDSPNADKGLVNESLNKE
                     IERALSTLTERERDIIKYFFGIGTNEMTLEEIGEHFGLTRERVRQIKEKAIRRLRHSA
                     RSKLLKSYLG"
     gene            2149..>3404
                     /locus_tag="P5293_07785"
     CDS             2149..>3404
                     /locus_tag="P5293_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRR49833.1"
                     /translation="MKRTPIFMMLVCLLTVVSCTNKVDNNPLLNEWKTPFDIPPFDQI
                     KVDHFMPAYIEAMKRHNEEIKAIVNNKEEASFENTIAAYDASGELLDRVSSVFSNVQG
                     TKSNDSIMSIAKELSPLVSKHYNEITMNAGLFKRIKSVYDKKDSLNLNPEQMRLVTEI
                     YKDFERSGANLPEDKKEELKKINQEIASLQLTFSQNLLTETKNFKLVIDNKKDLEGMS
                     PSLIAAAAERAAKEGLEGKWVFGLDNPSIMPFLQQSRNKEQRKQILDAYLNRCNNNNE
                     YDNKEIIKKLIKLRLDKAKILGYENFAQYQLDDRMAKTPEAVYDLLNKLWTPSLKVAA
                     NELKDIKAVAESEGYKGEIGPEDWRYYFEKSLSKKYNISDSLLRPYFKLENVREGIFY
                     VANQLYGITFTPLDSVPLPNDETTAY"
BASE COUNT         1186 a          534 c          711 g          973 t
ORIGIN      
        1 ttttctttgg atacaataat cctcaacctt tgcaaagaga acagcttact gcaggttcgg
       61 gcgttatcat tcatcctgac ggatatatcg tgacaaatag tcatgttgtg tcaggagctg
      121 atgagatttc tgtaactctt gaaaacaatc aaacatataa ggcaaagtta attggggcag
      181 atgaagcaac agacatagcc cttattaaaa ttgatgctac agggttacca tatcttgaat
      241 ttggcgattc tgactctctt aggttgggag aatgggtaat agctatcggc aacccataca
      301 atttgacgaa tacaattaca gcaggcattg tcagcgccaa gagaagaagc atgtcttctt
      361 atgacagtaa ctttagtgat ggtaaattta aaatagaatc atttattcag acagatgcag
      421 ctgtaaatcc agggaacagt ggtggagcac ttgtgaatgc aaagggagaa ttggttggta
      481 ttaacacagc tattgcatca agaactggct cattcacagg ctactctttt gcagtcccag
      541 cttctatcgc caaaaaagtt gtcaatgact ttataaattt cggagaggta aaaagggcaa
      601 tgttgggtgt ttccatgcag gagattgaca gcgaagcggc taaacgtatg aacatgaaag
      661 aaataaaagg agtacttgtc acagatgtta tgaagggagg agctgcagaa aaatctggaa
      721 taaaaccatc ggacgtcatt ctttcaatca atggacagcc tgtcaattca tcaccggcag
      781 ttcaggaaca aataagtaaa tacaggccga atgataaaat taatgttgaa attcttcgag
      841 atggtaagcc taaattaatt tcagttattt tgcaggagga aaatagtaaa ttattgtatg
      901 aaggaagtat taatgctgaa ttgcaaaaca ctataggagc aactttacaa aaagcttcaa
      961 aagaaactct taaaaaactt tcgcttagaa atggagttga agtaacttct gtgggcagcg
     1021 gaaaattcag agaggcaggt atcaaacctg ggtttattat tacatatatt aatcaggtag
     1081 aagttaatga accatctgaa gtacttgcaa ttattaagaa aagtggcagg tctttgcttg
     1141 ttgaaggtgt ttatcctgac ggcagagtag tttactatgg tatgggactt taattttgtg
     1201 gcttagatta atttgtttta actttgtagt acagtatatt tgaatgagac aacttaagat
     1261 taccaaatca attaccaaca gagagagcgc ttctctcgac aagtatttac aagagatagg
     1321 aagagaagaa ttaattacgg ttgaagacga agttgaattg gctcaaagga tcaaaaaggg
     1381 cgatcaggaa gctcttgaga aactcactag agcaaatctt cgttttgtcg tttctgtggc
     1441 taaacagtat caaaaccaag gcttaagtct tcctgatttg attaatgagg gcaaccttgg
     1501 cttgatcaaa gcggcagaaa agtttgatga aactagaggt tttaaattta tttcatatgc
     1561 agtctggtgg atacgtcaat ctattcttca agctcttgca gaacagtcaa gaattgttcg
     1621 cttaccgctc aatcaggtag gttcgcttaa taaaattaat aaagctctat caaagtttga
     1681 gcaggagaat gaacgaatgc cgtcaccgga agaacttgca gaaattcttg atattcctaa
     1741 agaaaagatt gcagatacat tgagagtttc agggagacac gtgtctgtag atgctccttt
     1801 tgtggatgga gaagataaca atttgcttga tgtgctggtt aacaatgatt ctccaaatgc
     1861 cgataaaggt cttgtcaacg aatctcttaa caaggagata gaacgtgctt tgtctactct
     1921 tactgaaaga gagagagata ttatcaagta cttttttgga ataggaacaa atgagatgac
     1981 actcgaagag attggtgagc attttggttt gacaagagag agagttagac agattaagga
     2041 aaaagctata agacgtttaa gacatagcgc aagaagtaaa cttcttaaat catatcttgg
     2101 ataattatat gcgtttttaa atttaaaata accccaaact ttaaacatat gaaaagaaca
     2161 ccaattttta tgatgttagt gtgtttatta acagttgttt cttgcactaa caaggtagac
     2221 aacaatcctc ttttgaatga atggaagaca ccattcgata ttcctccttt tgatcaaata
     2281 aaggttgatc atttcatgcc tgcatatatt gaggccatga aaaggcataa tgaagagatt
     2341 aaagctattg ttaacaataa ggaagaagct tcatttgaaa atactatcgc agcttatgat
     2401 gcatctggag aactgttaga cagagtttct tcagtttttt caaatgtgca ggggacaaaa
     2461 tctaacgaca gtattatgtc tattgccaaa gagctttctc ctcttgtcag caagcattac
     2521 aacgaaataa ccatgaacgc cggacttttt aaaagaatta aaagcgttta cgataagaaa
     2581 gactcattaa atttgaatcc tgaacaaatg agattggtta cagaaattta taaggatttt
     2641 gaaagaagtg gtgccaatct tcctgaggac aaaaaagagg agttgaaaaa gattaatcag
     2701 gaaatagctt cactacaact gactttttct cagaatttac ttactgaaac aaaaaatttc
     2761 aaacttgtaa ttgataacaa gaaagacttg gaagggatgt cgccatcttt aattgctgca
     2821 gctgcagaaa gggcagcaaa agaaggtctt gaaggaaagt gggtattcgg ccttgacaat
     2881 cctagcatta tgcctttcct ccaacagtca agaaataagg aacaaagaaa gcagattctt
     2941 gatgcatatc tcaatagatg caacaataac aatgaatatg acaacaagga gattatcaaa
     3001 aaactgataa aattgagact ggacaaagcc aagatactag gttatgaaaa ttttgctcag
     3061 tatcagcttg atgacagaat ggccaaaaca cctgaggctg tgtatgatct gcttaataaa
     3121 ctatggactc cttcccttaa agttgctgca aatgagttaa aagatattaa agcagttgcg
     3181 gagtcagaag gttataaggg agaaattggc cctgaagatt ggagatacta ttttgaaaag
     3241 agtctttcaa agaaatataa tatcagtgac tcactgcttc gtccttattt taaattggag
     3301 aatgtgagag aaggcatttt ctatgtggca aatcagctat atggtataac ttttacacct
     3361 cttgattcgg taccacttcc taatgacgag acaactgctt atga
//
LOCUS       DAOTQL010000155         3293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_22473_length_3293_cov_3.558678, whole genome shotgun sequence.
ACCESSION   DAOTQL010000155 DAOTQL010000000
VERSION     DAOTQL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22473_length_3293_cov_3.558678"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="P5293_07790"
     CDS             <1..799
                     /locus_tag="P5293_07790"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRR49834.1"
                     /translation="IKSKNSDLLRYIPGFVIDWVERLICQDEINNILDKYGNLKGVPF
                     ATATLRELNATYSVSGMDKIAHDGRYIIVSNHPLGGFDGMILMDIFGNYYNNKIRFVV
                     NDLLSAVEPLKPIFLPVNKFGKQSVEIAEKIHQEYTSDNQIITFPAGLCSRLINGRII
                     DLEWKKSFVNQAIKYKRDIIPIYFDGKNSNFFYRFANIRKKLGIKFNYELILLPREMF
                     KQRGGKFNIYIGDPIHCEELAHNGTPAEWTAKIRKTVYSLKPLKWNQ"
     gene            787..1929
                     /locus_tag="P5293_07795"
     CDS             787..1929
                     /locus_tag="P5293_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRR49835.1"
                     /translation="MEPVIPPVDRKLIIDELTSDKFIRHTRKGDNRLYEVTAHDSPHT
                     MNEIGRLREIAFRLSGGGTGLSVDIDSFDTNSFTPYKQLIVWDPKDQEIIGGYRYIHC
                     AGLDIEKMATHELFEFSDQFKKDYLPFTIELGRSFIQPNYQGTNIRHKGLYSLDNLWD
                     GLGALLVKYPKIKYFFGKVTMYTTYNQRARNMLLTFLNTFFPDNEGLVKPYKPLEFDS
                     ENPYYKELFNPQDYKESYRVLSKEIRLLGENIPPLINSYMNLSPTMKVFGTSINESFG
                     GVEETGILININQIYPEKIERHVSPILQIANKLKSTWWNVTKPIISTIKNEGQQDAEK
                     KKNVQKSNNVRKIVRNITEKPLTPKNKKGRKSSTGSKDSSTEPEDN"
     gene            complement(1895..2419)
                     /locus_tag="P5293_07800"
     CDS             complement(1895..2419)
                     /locus_tag="P5293_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRR49836.1"
                     /translation="MKRITVTLFVIVSAVLISYSQEIVKGQDTVFVRKGPLLLDSTLL
                     NRNIFALLKEPGLSGNFVTIEQSQSIESLLLTYISNSSGRKIYGYRVRIFFDNKQDAR
                     SRSEAIVTQFTSQFPDIRAYASYEKPFFKVTVGDFRTKSEAMQLLKRIEGMYRSVFLV
                     KEVINYPPVQYCYL"
     assembly_gap    2459..2468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2469..2898)
                     /locus_tag="P5293_07805"
     CDS             complement(<2469..2898)
                     /locus_tag="P5293_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49837.1"
                     /translation="MKKFLTVVLIVILAANGCTNYRNVDIKSVQLSSFKFVSTSKANI
                     ELSYIISNPTSSTIIFSSGNGIITRKGVDFAQLEVVRPDTLLPRSVKSGKIEINALLV
                     DPLPLLSMGLNIEKWRVEDFKLSTKLVFKNARGGKKILRYK"
     gene            2960..>3293
                     /locus_tag="P5293_07810"
     CDS             2960..>3293
                     /locus_tag="P5293_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822519.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRR49838.1"
                     /translation="MPNKYHSVRPLFNSSLPQLFIETYGCQMNVNDSEVVLSILQEAG
                     YALCNKIEDADLILVNTCSIRDNAEQRIWGRLEHFKTMKKKKSSLTIGILGCMAERLK
                     EELLNHPAV"
BASE COUNT         1162 a          585 c          565 g          971 t
ORIGIN      
        1 aataaaatca aaaaattctg atcttctgag atatatcccc ggatttgtta ttgactgggt
       61 agagagattg atctgtcaag atgaaattaa taatattctt gacaagtacg gcaatcttaa
      121 aggtgttcct tttgcaactg ccacacttag ggagttaaat gcgacataca gtgtttccgg
      181 aatggataaa atagctcatg atgggcggta tattattgtc tccaaccatc ctcttggagg
      241 atttgacgga atgatattaa tggatatttt tggaaactat tataataata aaatacgttt
      301 tgtggtaaac gatttacttt ctgcagtaga accattaaaa ccaatttttt tgccggtcaa
      361 taaattcggc aagcaaagtg tagaaattgc agaaaaaata catcaggaat atacctctga
      421 taatcaaata ataacattcc cagcaggatt atgttcaaga ttaatcaatg gcagaataat
      481 agacctcgaa tggaaaaaaa gctttgttaa ccaggctatt aaatataaaa gagatattat
      541 ccctatctat tttgacggta aaaattcaaa ctttttctac aggtttgcta atattagaaa
      601 aaaacttggt attaaattta attacgaact catattgctt cctcgtgaaa tgttcaagca
      661 acgcggtggt aaattcaata tttatatagg cgatccaatt cattgcgaag aacttgctca
      721 taatggaaca cctgctgaat ggacagcaaa gataagaaaa acagtttact ctctaaagcc
      781 tttaaaatgg aaccagtaat acctcctgtt gatagaaaac tcataattga tgagcttaca
      841 agtgacaagt ttattcgtca taccagaaaa ggagacaaca gactttacga agtgacagca
      901 catgattcac cacacaccat gaatgaaata ggccgtctta gagagattgc tttcagacta
      961 tccggcggag gaacaggcct gtcagtagat attgacagtt tcgacaccaa ttcttttaca
     1021 ccttataaac aactaattgt atgggatcct aaagatcagg aaattattgg tggctacaga
     1081 tacattcatt gtgcaggatt ggatattgaa aaaatggcca cacatgaact tttcgaattt
     1141 tcagaccaat tcaaaaagga ttatcttcct ttcaccatag aacttgggag atcatttatt
     1201 cagccaaatt atcaaggtac aaatattaga cacaaaggtc tatattctct tgacaatttg
     1261 tgggacggat tgggagcact tttagtaaaa tatcctaaaa tcaagtactt ctttggaaag
     1321 gtaacaatgt ataccactta taatcagcgt gcaagaaata tgctcctaac tttcttaaac
     1381 acattttttc ctgataacga aggcctcgta aaaccataca aaccccttga gtttgactct
     1441 gaaaacccct attacaaaga actttttaac cctcaagact acaaggaatc ttatagggtc
     1501 ctttcaaagg aaataaggtt actaggagag aatatacctc ccctcattaa ctcttatatg
     1561 aacctgagtc caactatgaa agtctttgga acatctatta atgaaagctt tggaggagtt
     1621 gaggaaactg gcatacttat aaatataaac caaatatatc ctgaaaagat tgaaagacat
     1681 gtttcaccaa tacttcaaat tgctaacaaa cttaagtcga catggtggaa tgtcacaaaa
     1741 ccaatcatat ctacgataaa gaacgaagga caacaagatg ctgaaaaaaa gaagaatgtt
     1801 caaaagagta ataatgttag aaaaatagta aggaacatta cagaaaaacc tttaactcca
     1861 aagaataaaa aaggtaggaa aagcagtact ggatctaaag atagcagtac tgaaccggag
     1921 gataattaat tacttctttt accagaaaaa cagaacgata catcccctct atcctcttca
     1981 aaagttgcat ggcttctgat ttcgtcctaa agtcaccaac agtaaccttg aaaaaaggtt
     2041 tttcatatga agcataagct cttatatcgg gaaattgaga ggtaaactga gttacaattg
     2101 cctctgaacg acttcttgca tcctgcttgt tgtcaaaaaa tattctgact ctataaccat
     2161 atattttcct tcctgaagaa ttggaaatat atgttaataa aagcgattct atgctctgag
     2221 attgctctat agtcacaaaa ttacccgaaa gtccgggttc ttttaataat gcaaaaatat
     2281 ttctgtttag caaggtggaa tcgagcagca agggaccttt acgtacaaaa acagtatctt
     2341 gtcccttgac aatttcctga gaataggaaa ttagtactgc tgaaacaatt acaaaaagag
     2401 ttactgtaat tctcttcata atttataatc tattaatcag gtttttaagg ggtatgtcnn
     2461 nnnnnnnncc ttatatctga gtattttttt acctcctctt gcattcttga aaactaattt
     2521 tgtcgataat ttaaaatcct cgactctcca tttttcaatg tttaatccca tcgacagcaa
     2581 aggtagagga tcaaccagaa gcgcatttat ttcaatttta ccacttttaa cacttcttgg
     2641 gagaagtgta tctggcctga caacttcaag ttgtgcgaaa tcaactcctt tccgagtaat
     2701 aatcccattg cccgaagaga atataattgt agaagaagtc ggattgctga ttatataact
     2761 gagttcaata tttgccttag aagtgctgac aaatttgaag ctacttaatt gtacggattt
     2821 gatatccaca ttcctgtaat ttgtacatcc attggctgcc aaaataacta tcaatacaac
     2881 tgtcagaaat ttcttcatct catttcagat tatctgcaaa ggtataaaaa aggctatatt
     2941 tgcagtacaa aatgcaaata tgcccaacaa atatcattcc gtaagaccac tatttaatag
     3001 tagtttgcct cagcttttca tcgagactta tggctgtcag atgaatgtta acgacagcga
     3061 agttgttctt tcgatacttc aggaagccgg ctatgctctt tgcaataaaa ttgaagatgc
     3121 cgatcttatc ctcgtaaaca cttgctcaat aagggataac gccgaacaaa gaatctgggg
     3181 aagacttgaa cacttcaaaa caatgaagaa aaaaaaatca agcctgacta tcgggatatt
     3241 gggatgtatg gcagaaagac tgaaagagga gttgctaaat catcctgcgg ttg
//
LOCUS       DAOTQL010000156         3290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_22491_length_3290_cov_4.309737, whole genome shotgun sequence.
ACCESSION   DAOTQL010000156 DAOTQL010000000
VERSION     DAOTQL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22491_length_3290_cov_4.309737"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="P5293_07815"
     CDS             <1..437
                     /locus_tag="P5293_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR49839.1"
                     /translation="LADFRNKKIGFVFQFHHLLPEFTSVENVIIPALIAGERLGSAKE
                     RAREILRLLGLGDRLSHKPSQLSGGEQQRVAIARALINQPSVIFADEPSGNLDTNTKR
                     EIHKLFFELRDRYNQTFIIVTHDRELASMSDRILEMRDGEFI"
     gene            491..1213
                     /locus_tag="P5293_07820"
     CDS             491..1213
                     /locus_tag="P5293_07820"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRR49840.1"
                     /translation="MKVNTDAVLLGAWAGIPLGNEPFFFLDIGTGTGVIALMIAQRLS
                     DLKLNFRATAIDPDHNSVNESAFNFANSPWASSLSAKEMTLEEFMLMEKGDCFDIIVT
                     NPPFFSNSLKSPSLRKSASRHNDILPFSTIVKASEYLLKQDGKLSLILPEEERLHFMS
                     EINKAAVASAPSLFLTRICQVKTLPCKEAKRALMEFTKFPADKSVMDVGGIIREELCI
                     YNSDRTSKSMEYEKLIGDFYLE"
     gene            complement(1238..1768)
                     /locus_tag="P5293_07825"
     CDS             complement(1238..1768)
                     /locus_tag="P5293_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49841.1"
                     /translation="MKRIVTLSFILCLSLLARGQVASSAFIKIEQAGKTEQGFRIDQE
                     KKVSKQQQIMSAKIAFFTTEMRLTPQEAATFWPVYNQYWEERENAHKRVQRNLEALRI
                     ILEDKRPNNEERLKKMLDAYINSQAIQGMIYRKYYEEFQKVLPIEKVARIYKAEEDFR
                     IKLIYELRNSGEKQKH"
     gene            complement(1778..2188)
                     /locus_tag="P5293_07830"
     CDS             complement(1778..2188)
                     /locus_tag="P5293_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49842.1"
                     /translation="MKEKINIDHPELRKNPFTVPANYFENLAEAVDEKISYVAPKVGW
                     WSVIKPQLALVSTFCIIFFMGYGAITLFTPDSAKKNSLQHPNQLEEYLLSSSSFDFYD
                     EEIDSLTTIKKIDPDQIVEYLRDDASLVYLASIK"
     gene            complement(2219..2761)
                     /locus_tag="P5293_07835"
     CDS             complement(2219..2761)
                     /locus_tag="P5293_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRR49843.1"
                     /translation="MDDKDLLILFREEGKERHAFDLIVRKYSERLYWHIKKMVISHDD
                     ANDLLQNTFIKAWNALPEFREESKLFTWLYRIATNEVLTFLKKKQLRSFLSLTDYSKQ
                     LENVLEADPYFNGDKTQKALQKAILNLPEKQRIVFNMRYFDELKYEEISEILGTSVGA
                     LKASYHHASKKIEEAMQKLI"
     gene            complement(2767..>3290)
                     /locus_tag="P5293_07840"
     CDS             complement(2767..>3290)
                     /locus_tag="P5293_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRR49844.1"
                     /translation="CDYNQTKAATIAVADYQGPVYLRFGRPSVPNFTPADQTFEIGKA
                     IRMIEGTDVTLFATGHLVWESILAAQELEKKGISAEVIDLHTIKPLDKITVLQSVQKT
                     KAVVVAEEHLIAGGLGELICGTLSREYPVPVELVAVNDEFGQSGTPEELMKKYGLDAI
                     SIVEAVNRVLKRK"
BASE COUNT          937 a          629 c          604 g         1120 t
ORIGIN      
        1 ctcttgccga ctttaggaac aaaaagatag gatttgtttt tcaatttcac cacttgcttc
       61 cggagtttac atctgttgaa aatgtgatta ttcctgcttt aattgctggt gaaaggttag
      121 gttctgcaaa agaaagagcc cgtgagatac tccggttgct cggacttggc gacaggcttt
      181 cccataaacc ttcccaattg agtggaggag agcagcagcg tgttgccatt gcaagagccc
      241 tgattaatca accatctgtc atttttgctg atgaaccatc gggtaatctt gatacaaata
      301 ccaaacgtga aattcacaag ctctttttcg aacttcgtga cagatataat cagacattta
      361 ttattgttac tcatgaccgg gaactagctt caatgagtga cagaatactc gaaatgcgtg
      421 atggagagtt tatttaatgc attctgtttt ttgttttaaa agatttaaag tacggcaaga
      481 atcttcaagc atgaaagtca atacagatgc tgttttgctt ggggcatggg caggcatacc
      541 tctaggaaat gaaccttttt tctttctcga tattggtaca gggactggag ttatagcttt
      601 aatgattgcc caaaggctat ctgacttgaa gttgaatttt agggcaacag ccattgatcc
      661 tgatcacaat tcggtaaatg agtctgcttt taactttgcc aattctcctt gggcttcttc
      721 tctttcagct aaagaaatga cactggaaga gtttatgctt atggaaaaag gagattgttt
      781 tgacattatt gtgacaaatc ctcccttttt tagcaattct cttaaatcac catcattacg
      841 caaatcagct tcaagacaca atgacatatt gcctttttca actattgtca aagcttcgga
      901 atatcttttg aaacaagacg ggaaactttc tttaatcttg cctgaagaag aaagattaca
      961 tttcatgagt gaaatcaata aggccgctgt tgcatcagcc ccatctcttt tccttacccg
     1021 gatatgtcag gttaaaaccc taccctgtaa agaagcaaag agggcgttga tggaatttac
     1081 caagtttcct gcagataaaa gcgtgatgga cgttggggga attattcgtg aagagctttg
     1141 tatttataac agtgacagaa cttcgaagag tatggaatat gaaaaactta taggggattt
     1201 ctatttagaa taggtaattt acaattaatt taattttcta atgtttttgt ttttctccac
     1261 tgtttcgtag ctcatagatc agtttaattc taaaatcttc ctcagccttg tatattctgg
     1321 caactttttc aattggaaga actttttgaa actcctcata atattttcga tagatcattc
     1381 cctgaatagc ctgactattt atatatgcgt ccagcatttt tttgagtctt tcttcattat
     1441 taggtctttt gtcctcgagg attatacgta gagcctcaag attgcgctga actcttttat
     1501 gggcattttc cctctcttcc cagtactggt tgtaaacagg ccagaatgtt gcagcttcct
     1561 gaggagtcag cctcatttca gttgtgaaga atgcaatttt tgcactcata atctgctgct
     1621 gtttagacac ctttttttcc tgatcaattc taaatccctg ctcagtcttg ccggcttgct
     1681 cgatttttat gaaagcggat gaagctactt gacctcttgc aagtaatgaa aggcaaagta
     1741 taaatgaaag tgtaacaatt cttttcatat gcataagtta tttgatcgaa gcaagatata
     1801 ctaaagaagc atcatctctc aagtattcta caatttgatc aggatcaatc ttttttattg
     1861 ttgtgagaga atcaatctct tcgtcataaa aatcaaaaga agaactgcta agcagatatt
     1921 cttccaattg atttggatgt tgtaaagagt ttttctttgc tgaatcaggt gtaaataaag
     1981 taatggcgcc atatcccata aagaagatta tgcagaatgt cgaaacaagt gcgagttgag
     2041 gtttaattac tgaccaccag cctacttttg gcgccacata agaaattttt tcatctaccg
     2101 cttcggcgag attttcaaaa taattcgcag gaacagtaaa aggatttttc cttaattcag
     2161 gatggtctat gttaattttt tctttcatac ctctttgact attcaaattt aaaaaagatt
     2221 aaatcaattt ttgcattgcc tcctctattt tctttgaagc atgatgatat gaagccttga
     2281 gagccccaac agatgtgccg agtatctctg aaatttcttc gtatttgagt tcatcaaaat
     2341 acctcatatt aaaaactatt cgctgttttt ctggcaagtt gagaatagct ttttgaagtg
     2401 ccttttgtgt cttgtctccg ttaaaatagg ggtcagcctc aagtacattt tcaagctgtt
     2461 tggaatagtc cgtcagtgat aaaaaagacc ttaattgttt ttttttcaaa aatgtgagga
     2521 cctcatttgt tgctattcta tatagccatg taaacagttt tgattcctct ctaaactctg
     2581 gaagagcatt ccatgctttg ataaatgtat tttgaagaag atcattggcg tcgtcatgcg
     2641 aaataaccat cttcttaata tgccagtaaa gtctttcgct gtatttacgg acaataagat
     2701 caaaagcatg acgctcttta ccctcttctc tgaagagtat gagtagatcc ttatcgtcca
     2761 ttgtctctac tttctcttta agactctgtt tactgcttca acaatgctta tggcgtcaag
     2821 gccatatttt ttcataagtt cttccggtgt gccactttgg ccaaattcgt catttacagc
     2881 taccaactcc acaggcacgg gatattctct tgacagagtt ccacagatta attctcctaa
     2941 gccgcctgca ataagatgtt cttctgcaac tacaacagct tttgtttttt gcacagattg
     3001 taagactgtt atcttgtcaa gaggtttgat agtatgaaga tcaattacct ccgcacttat
     3061 accttttttt tcaagttcct gtgctgcaag aattgattcc catacaagat gtccggtagc
     3121 aaataatgta acatcagttc cctcaatcat tcttattgct tttcctatct caaaggtctg
     3181 gtctgcaggt gtaaaattag gtacagaagg tctgccaaac ctgaggtaaa caggcccttg
     3241 ataatcagca acagcaattg tagctgcctt tgtctgattg taatcacaag
//
LOCUS       DAOTQL010000157         3258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_22794_length_3258_cov_3.500781, whole genome shotgun sequence.
ACCESSION   DAOTQL010000157 DAOTQL010000000
VERSION     DAOTQL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22794_length_3258_cov_3.500781"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="P5293_07845"
     CDS             <1..605
                     /locus_tag="P5293_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRR49845.1"
                     /translation="QAILPLRGKILNVEKAMEHKVFESEEIRNIYTALGVTVGTEEDS
                     KALNLSKLRYHKVIIMTDADVDGSHIATLILTFFFRYMQDLIKNGHVYIATPPLYLVK
                     KGKQEQYCWTEDERKAAIEKLGAGKESGLHIQRYKGLGEMNAVQLWDTTMNPETRILR
                     QVDIENAAEADRIFSMLMGDDVPPRREFIETHAKYANIDA"
     gene            598..1524
                     /locus_tag="P5293_07850"
     CDS             598..1524
                     /locus_tag="P5293_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRR49846.1"
                     /translation="MPDTDNIKLFSLFLLLLTFSVAISAEGKSKKRDFPFLPVRPVLL
                     LPEIPVAVPNYSGDIFGLNYSKPIPELIDFERCNEFSDIPEEYDIWSDTKINPYQVDL
                     VDMKDTVILDLSGYFPPSSKYVTSNFGFRKWRYHYGIDLKVMKNDSVYCAFDGVVRLT
                     RYDRRGYGYYVLVRHFNGLETLYGHLNKILVNPGDTVSSGGILGLGGSTGRSTGYHLH
                     FETRYLGNPINPNDLINFDTQSVSSEKYKLSAQNFLYKKEISKRRYWTIRRGDTLGKI
                     AMRTGVSVSKLCALNGIKKNTILRIGRKLRYT"
     gene            1654..>2016
                     /locus_tag="P5293_07855"
     CDS             1654..>2016
                     /locus_tag="P5293_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR49847.1"
                     /translation="MIFDGKEVLAWTFNNYLGLANDPEVRKVDAEAAAQWGLGYPMGA
                     RIMSGHTKYHEQLERELADFELKEDAFLFNFGYQGMVSTIDALVNRHDIIVYDSEAHA
                     CIMDGVRLHMGQRIVYPHN"
     assembly_gap    2017..2116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2117..2647
                     /locus_tag="P5293_07860"
     CDS             <2117..2647
                     /locus_tag="P5293_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR49848.1"
                     /translation="DLYFGAFAKSMASIGGFVAGPRNIINYLRYNARSQIYAKALPMP
                     FVIGNLKRLELIRTRPELREKLWTITRALQNGLRERGFDIGKAEACVTPVFLKADVVE
                     ATNICIDLRDNYKIFCSVVVYPVVPRDVIMFRIIPTAMHSLEDVQYTLDVFSKISGKL
                     KSGAYKGTEMKNKAIE"
     gene            2678..>3258
                     /locus_tag="P5293_07865"
     CDS             2678..>3258
                     /locus_tag="P5293_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR49849.1"
                     /translation="MKDKVVIVTGASSGIGLAAVKAFASEGAKVVMAARRLDKLLEIE
                     KQLSDITETLVVKTDVSLEEDCKKLVEQTIKRFGRIDVLVNNAGISMRALFIDLDLSV
                     IKRLMDVNFWGTVYCTKYALPYILKNHGSIIGVISIAGFKGLPARTGYSASKFAIYGF
                     LETLRIEHLKDNLHVMIFAPGFTASNIREEALVS"
BASE COUNT         1040 a          574 c          654 g          890 t
ORIGIN      
        1 tccaggcaat tcttccactt cgaggaaaaa ttctcaatgt tgaaaaagct atggaacata
       61 aagtttttga aagcgaagaa atcagaaata tttatacagc tcttggagta actgtaggaa
      121 cggaagaaga cagtaaggca cttaacctct caaaactacg atatcacaaa gttataatta
      181 tgacagatgc agatgttgat ggaagtcata tagccacact tattcttact tttttcttca
      241 ggtatatgca ggatctaata aaaaatggcc atgtatacat agccacacct cctctatatc
      301 ttgtcaaaaa aggcaaacag gagcagtatt gctggacaga agacgaaaga aaggcagcaa
      361 tagaaaaact gggagccgga aaggaatcag gactgcatat tcagagatac aaaggtcttg
      421 gagagatgaa tgccgttcaa ctttgggaca caactatgaa tccagagacc aggattttgc
      481 ggcaggttga tattgagaat gctgcagaag cagacagaat attttcaatg ctgatgggcg
      541 acgacgtgcc gccaagaaga gaatttattg aaactcacgc aaaatacgcc aatatagatg
      601 cctgatacag ataacattaa gttattctca ttgtttctgc tgctcttgac attttctgtt
      661 gcaatttcag cagaaggaaa atcaaaaaaa cgtgattttc cttttttgcc tgtaaggcct
      721 gttctccttt tgcctgaaat acctgttgca gttccaaact actctggcga catctttggg
      781 cttaattatt caaagcctat tccggaacta atagattttg aaaggtgcaa tgagttttca
      841 gatatacctg aagagtacga catttggagt gataccaaaa taaatcctta tcaggtcgat
      901 cttgtagata tgaaagatac cgtaattctt gatctatctg gatactttcc tccatcatcc
      961 aaatacgtaa cttccaattt tggattcaga aaatggaggt atcactatgg gatagatcta
     1021 aaagtgatga aaaacgactc tgtatattgt gctttcgatg gtgttgtcag gttgaccagg
     1081 tacgatagaa gaggatacgg ctattatgta cttgtcagac acttcaacgg tcttgaaact
     1141 ctttacggtc atcttaataa gattcttgta aatcctggag atactgtgtc ttccgggggc
     1201 attcttggtc tgggcggaag taccggtaga tctaccggtt atcatctgca tttcgaaaca
     1261 agatatcttg gcaatccaat taatccgaac gatcttatca actttgacac acaaagtgtc
     1321 tcaagtgaaa aatacaagct ttcggctcaa aattttttat acaaaaaaga aatctcaaag
     1381 aggcgctatt ggacaatacg tcgtggagat actctcggta aaattgccat gagaacaggt
     1441 gtctcagttt caaaactttg tgctctcaac gggatcaaga agaatacaat actcagaata
     1501 ggaagaaaat taagatatac ataaaattaa ataaatcaca tggacatttt tgaaagtata
     1561 aacaatgatg aaggagatcc actaggacaa tacagaagca agggtcatgg ttagtttact
     1621 tatcccaagc tagaagggcc catacaacct aaaatgattt ttgacggaaa agaggttctc
     1681 gcatggacat tcaacaatta ccttggtttg gcaaatgatc ctgaagtcag aaaagttgac
     1741 gcagaagccg ctgcacaatg gggacttggt tatcctatgg gtgcacgcat aatgtccgga
     1801 cacacaaaat atcatgaaca gcttgaaaga gaacttgctg attttgaact caaagaagat
     1861 gcctttctat ttaattttgg ctatcaggga atggtttcaa caatcgatgc gcttgttaac
     1921 agacatgata taattgttta tgattcagaa gcccacgcat gtataatgga tggggtcaga
     1981 ctccacatgg ggcagagaat tgtttatcct cacaacnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnngacc tctactttgg agcatttgca aaatctatgg cctcaatagg
     2161 cggctttgta gccggaccaa gaaatatcat aaactatcta cgttataacg cacgctcaca
     2221 aatttatgca aaggctcttc caatgccatt tgttataggt aatttgaaac gtctcgagct
     2281 cattcgtaca agaccggaac ttcgcgaaaa gctttggaca attacaagag cactgcaaaa
     2341 tggccttaga gaaagaggat ttgatatagg taaggcagaa gcttgcgtaa ctcctgtatt
     2401 cctcaaagct gacgttgttg aagcgactaa catatgtatt gatctaagag ataattacaa
     2461 gattttttgt tctgtcgtag tttatcctgt tgtgccaaga gacgtgataa tgttccgaat
     2521 tattcctaca gcaatgcatt ctcttgaaga tgttcaatat acactcgacg tattttcaaa
     2581 aatctctggc aagctgaaaa gtggggcata caagggcacc gagatgaaga ataaggcaat
     2641 cgaatagaaa caattatttc ttgtgcacat gtacgttttg aaagataaag ttgttatcgt
     2701 aaccggtgcc agctcaggta taggcctggc tgccgttaaa gcttttgcat cagaaggtgc
     2761 aaaagttgtc atggcagcca gacgacttga caaactgctc gaaatcgaga aacagttgtc
     2821 agatattaca gaaacacttg tagtcaaaac agacgtgtca ctcgaagaag attgcaaaaa
     2881 actcgttgaa cagacgatta aacggttcgg gagaatagat gtactggtaa acaatgcagg
     2941 aatatctatg agagcactct ttatcgattt agacctttcg gttatcaaaa gattgatgga
     3001 tgtaaatttt tggggtacag tatattgtac gaaatatgct ttgccctata ttttaaaaaa
     3061 ccatggttct attattggag tgatatcaat tgcaggtttt aaaggtctgc cagctcgtac
     3121 aggctactct gcatcaaaat ttgcaattta tggctttctc gaaactctca gaatagaaca
     3181 tttaaaagac aatctgcatg taatgatttt tgctccaggg tttactgcat caaatatcag
     3241 agaagaagca cttgtctc
//
LOCUS       DAOTQL010000158         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_23648_length_3162_cov_4.206630, whole genome shotgun sequence.
ACCESSION   DAOTQL010000158 DAOTQL010000000
VERSION     DAOTQL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23648_length_3162_cov_4.206630"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="P5293_07870"
     CDS             <1..381
                     /locus_tag="P5293_07870"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRR49850.1"
                     /translation="RYIDAPSGLPLIQHSFQIMWDLHKKGYFSKFDVIERLSHSPANN
                     FNIVKRGFLREGYYADILIFNPNLEDSITTNNPAYKCGWSPFKNHTFSSSIIHTFVNG
                     IQVVKNGKLTGDKGGKRLEFSYEK"
     gene            371..1276
                     /locus_tag="P5293_07875"
     CDS             371..1276
                     /locus_tag="P5293_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRR49851.1"
                     /translation="MKNRESAIKFIPIIIATILWGFSFIWTNQLLQNQIPVFTIIFIR
                     LSLAAIILSTVSIFLKQLQKIERKDIPWFLMLVFCEPFMYFIGETFGLKAVNSPTIGA
                     IIVATIPVFSMIAGIYFYKERISKLNILGILMTLPGILLVVLENGWDTKVSLNGILLL
                     FLAVFSATGYSVVVRRLSDKYNSYTIVSFQNIIGAIYFLPLFLIYNGKDFAFERILSI
                     KFIYPILALAALCSVLAFVFFVNSIRVLGVARTNFFSSLIPAVSAAGAFMLGQEGMSY
                     TKAVGIVVVIIGVIIAQRDKKSNLT"
     gene            complement(1263..>3162)
                     /locus_tag="P5293_07880"
     CDS             complement(1263..>3162)
                     /locus_tag="P5293_07880"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRR49852.1"
                     /translation="LTSDLKELILSTIDANKLEDLYLPYKPKRKTKASIAKARGLEPL
                     AYKIFSFSLENCEAEAEKYLNEDVEDVDSALSGARDILAEWISENSNIRERLRREYLK
                     KGKIVSSPQKNVKEVDESKYKNYFNFTGDLNNIPSHRLLAILRGFNEGELVYKIDIDQ
                     QLPVEIIRKEIIPSDKRCSRSALKQVEIAIEDSYKRLLAPSIENEALKIAKEKADLES
                     VKVFGENLSALLLAPPVGEKRVLAIDPGFRTGCKIVCLDSQGKLLHNDTIYPHPPQNE
                     KIASIKKISNLIESYKIEVIAIGDGTASRETESFIKKIAMPPGLQVYMVSEDGASVYS
                     ASPVAREEFPNYDVTVRGAVSIGRRVMDPLAELVKIDPKSLGIGQYQHDVDQTLLREM
                     LDNTVVSCVNSVGVNLNTASKHLLKYVSGIGPSLAQSIVDYRSENGPFRSRYELKKIK
                     RLGDKAFEQCAGFLRITNAANPLDNSAVHPERYALVEKMAKDVNCSVSDLIREDQIRR
                     QVNVRKYIDKEVGLPTLNDIMAELAKPGRDPRRIATVMEFAPDVQTIEDLRVGMILPG
                     IITNITNFGAFVDIGIKQDGLIHISQMSDKFISSPNEVLKLHQHVKVRVIEVDLKRGR
                     IQLSLKSN"
BASE COUNT          934 a          621 c          513 g         1094 t
ORIGIN      
        1 aggtatattg atgctccgtc aggacttcca cttattcagc acagttttca gataatgtgg
       61 gatcttcata aaaaaggtta tttttccaag tttgatgtaa ttgaaaggct ttcccactct
      121 cctgcaaata attttaatat tgtaaaaaga ggcttcctca gagaaggata ctatgcagac
      181 atactaattt ttaacccaaa tcttgaagat tctatcacta cgaacaatcc tgcatataaa
      241 tgtgggtggt caccatttaa gaatcataca ttcagttctt caataattca tacatttgta
      301 aatgggatac aagttgtgaa aaacgggaaa cttactggag acaaaggtgg aaagagatta
      361 gaatttagtt atgaaaaata gagagtctgc aattaagttt attcctatta taattgccac
      421 tatcctttgg gggttttctt ttatctggac caatcaactt cttcaaaacc agatccctgt
      481 ttttactatt attttcataa gattaagcct tgcggctatt attctatcga ctgtttccat
      541 cttcctcaaa cagttacaaa aaatagaaag aaaggatatt ccgtggttcc tgatgctagt
      601 attttgtgaa cctttcatgt atttcatagg agagaccttt ggattaaaag ctgttaactc
      661 accgactatt ggcgcaatca tagtagccac aataccagta ttttccatga ttgccggcat
      721 atatttttac aaggaaagaa tttcaaaact aaatatactt ggcattttaa tgactttacc
      781 gggaattttg ctggtagttt tagaaaacgg ttgggatact aaagttagtt taaatggtat
      841 tcttcttttg tttctcgccg ttttttctgc cacaggatat tcagtagtag tgagacgatt
      901 atcggataaa tacaatagtt atacaattgt atcattccaa aacattatcg gagccattta
      961 ctttcttcct cttttcctga tttataatgg taaagacttc gcttttgaga ggattctatc
     1021 aataaaattt atatatccca tacttgctct tgctgcgtta tgttcagtcc ttgcctttgt
     1081 attctttgtc aattccataa gagttctggg tgtcgcaaga acaaatttct tttcttccct
     1141 gattcctgct gtttctgctg caggagcttt catgcttgga caagaaggaa tgagttatac
     1201 aaaagctgtc ggtatagtag ttgttataat tggtgtaata atcgctcaaa gagataagaa
     1261 atctaatttg acttaagact aagttgtatc ctacctctct tcagatcaac ttcaattact
     1321 ctgaccttaa cgtgttgatg aagtttgagc acctcattgg ggcttgaaat aaatttatct
     1381 gacatttgtg aaatgtggat tagcccatcc tgtttaatac ctatatctac aaatgcccca
     1441 aaatttgtaa tatttgtgat gattccaggt aaaatcatac ccactctcag atcttctata
     1501 gtttgaacgt caggagcaaa ttccataaca gttgctatac ggcgaggatc tcttcccggc
     1561 tttgccagct ctgccataat atcatttaaa gtaggaagcc ctacttcctt gtctatatat
     1621 tttctgacat taacctgcct tctgatttga tcttctctta tcagatcaga cactgaacag
     1681 tttacatctt ttgccatctt ttctacaagt gcgtatcttt ctgggtggac agcagagttg
     1741 tcaagaggat tggcagcatt agttattctt aagaaaccgg cacattgttc gaaagcctta
     1801 tctccaagcc tcttaatttt tttaagctca tatctagacc tgaaaggacc attttcgctt
     1861 cgatagtcca caatactttg agccaaggat ggacctatcc ctgaaacata ttttagcaaa
     1921 tgcttacttg ctgtgtttaa attaactccc acactattta cacaacttac tacggtatta
     1981 tcgagcatct cccgaagtaa tgtctgatcc acatcatgct ggtattgtcc aattccaaga
     2041 gattttgggt caattttaac tagctcagcc aaaggatcca ttactcttct tcctattgaa
     2101 acagcacctc taacagtaac atcataatta ggaaactctt ctctcgcaac aggagaagca
     2161 gaatatactg atgctccgtc ctcacttacc atataaactt gtaatcccgg aggcatggct
     2221 attttcttaa taaaagattc tgtttcccga cttgcagttc cgtcgccaat agcaattact
     2281 tcaattttat atgactcaat gagatttgat atttttttta ttgaagcaat cttttcattt
     2341 tgaggaggat gaggataaat tgtatcatta tgtaacaatt taccttgaga gtcaagacaa
     2401 acaatcttac agccggttct aaaaccaggg tcaatagcta aaactctctt ctcacctaca
     2461 ggtggtgcaa gtaaaagtgc tgacagattt tcaccaaaaa ctttaactga ttctaaatct
     2521 gctttctctt ttgcgatttt aagagcctca ttctcaatgg aaggagcaag aagtctttta
     2581 taggaatctt caatagcaat ttctacttgc tttaatgcac ttctactaca tcttttgtca
     2641 gacggaataa tttcttttcg aatgatctca acaggcaatt gctggtcaat atctatctta
     2701 taaaccaact caccttcatt aaaccctctc aaaatcgcca aaagcctgtg agatggaata
     2761 ttattcaaat cacctgtgaa attgaaatag tttttgtact tgctttcatc cacctcttta
     2821 acattttttt gcggagatga aactatttta ccttttttta gatattcccg cctcaatctc
     2881 tctctaatat ttgaattttc actaatccat tcggctaata tatctcttgc ccctgaaagt
     2941 gctgaatcca catcttcaac atcttcatta agatattttt ctgcctcggc ctcacaattt
     3001 tcgagtgaaa aagaaaaaat tttgtaagca agaggttcca gacctcttgc tttggcaata
     3061 gaagcttttg tttttctttt gggcttataa ggaagataca aatcttcgag tttgtttgca
     3121 tcgatagtag ataatatcaa ctctttcaga tcggaagtca at
//
LOCUS       DAOTQL010000159         3134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_23908_length_3134_cov_3.673595, whole genome shotgun sequence.
ACCESSION   DAOTQL010000159 DAOTQL010000000
VERSION     DAOTQL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23908_length_3134_cov_3.673595"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="P5293_07885"
     CDS             complement(<1..422)
                     /locus_tag="P5293_07885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRR49853.1"
                     /translation="MNQLIIIIILLILALPIVASFKIGWGVYIRSICKLDTKERIIAI
                     TFDDGPDDKNLPKLLDILDKKGVKSTFFFIGEAISKKPDMLIRTINEGHKVGIHTYSH
                     SPFFPFYSLNKIVYEIQQTRLLIKSISKIETSLFRPPFG"
     gene            complement(358..1320)
                     /locus_tag="P5293_07890"
     CDS             complement(358..1320)
                     /locus_tag="P5293_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HRR49854.1"
                     /translation="MMNKRIYILNCATVEGKNEPDYKDYIPDPLARRRMSRIIKMGLT
                     SAMIALKDSGLESIDGIITGTGWGCLADTERFLNSIYENDERLLNPSAFIQSTPNTIG
                     AQIALSLGINCYNVSYVHGGISFESALTDAMLMLKERNDMNLLVGGFDEMSPTKEHIL
                     GRMGVWRVTTPGEGAHFFVLGNSFREKALAEIESVTTFNENGALNSDIDLMNILERTE
                     ISLDSSCRVLYGLTNRLSFPEQLKDYLIDYKKLCGEYPTASAYALWYGSHLFKKMGNV
                     EKIIIVSRHLKERISVIVIKKFLHEPIDYHYNIIDTSSSDCCLV"
     gene            complement(1326..2540)
                     /locus_tag="P5293_07895"
     CDS             complement(1326..2540)
                     /locus_tag="P5293_07895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRR49855.1"
                     /translation="MMDNKVIVSGLGVISALGVGVNENLDSLMTLKSGIGPITLFQTN
                     HDVMVGEVRLGNMEMIEMLCLDSSKRYSRTALLGLIAAREAYSDSGFLFLGQQERMRL
                     KIGVISSTSVGGMDVSENFYREYRLDKSKGRLRDLIGHDCGSSTKIIADYLKADDFVS
                     TVSTACSSASNAIMLGTRMIKNGLLDVAVVGGTDSLSRFTVNGFYSLGILDKEQCRPF
                     DETRAGLNIGEGAGYLIIQRSDIPHEKTYGEVCGYANANDAYHQTASSSDGTGAYLAM
                     SGALEVSGLSPDDISIVHAHGTGTPNNDASEGSALLRIFGNNVPLFSSTKSYTGHTLA
                     AVGGVESVFSLLSLKYGYVWPNLNFGVRMKDVDVMPVVKLVSGMDLKYVLKNSFGFGG
                     NCTTLIFGYCNR"
     gene            complement(2537..>3134)
                     /locus_tag="P5293_07900"
     CDS             complement(2537..>3134)
                     /locus_tag="P5293_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49856.1"
                     /translation="GGCNAAVIYSFDSVSKSVNSIYSSSWKEISRFRLSGDAGFHDII
                     RDWNRSLDNKDIKFFKMDDLSKLGYIGAIALMRNFPEIDNFPPEKRALFFANSSSSLA
                     SDLKHMAAIGRENDLLASPAIFVYTLPNTVLGEISIKNRFKGENTFFVHDPKRKFDVE
                     SLLLRDASLSSMDLVIAGWCELLGEHFDLNIALYTKDV"
BASE COUNT         1037 a          681 c          468 g          948 t
ORIGIN      
        1 ccaaatggag gtctgaaaag tgaagtttct attttagata tgcttttaat cagcaatctt
       61 gtctgctgaa tctcataaac aatcttgttt aaagaataaa aagggaaaaa aggtgaatga
      121 ctgtatgtgt gaattccaac tttatggcct tcatttatag ttcgtatcag catgtccggt
      181 ttttttgaaa tggcttcacc gataaaaaag aaagtagatt ttacaccttt tttatccaaa
      241 atatcaagaa gtttaggaag atttttgtca tccggtccat catcaaaagt aattgctata
      301 attctttctt ttgtatccaa cttgcatata gatctaatat atacacccca tccgatttta
      361 aacgaggcaa caatcggaag agctagtatc aataatatta taatgataat caattggttc
      421 atgaagaaat tttttaatca caattactga aattctctcc ttcaaatgtc gtgaaacaat
      481 tatgattttt tctacatttc ccatcttctt aaaaagatga ctgccatacc aaagagcata
      541 agccgaagct gtaggatact ccccacataa ttttttataa tcaatcagat aatcttttaa
      601 ttgctcagga aaagatagtc tgttagtcag gccataaagc actcggcatg aagaatcgag
      661 agaaatttct gttctctcga gtatattcat cagatcaata tcagagttaa gagcaccatt
      721 ttcgttaaaa gttgtaacac tttctatttc agccagagct ttttctctga aagaattccc
      781 aagaacaaaa aaatgtgccc cttcccctgg tgtagttacc ctccatacac ccattctgcc
      841 aagaatatgc tctttagtag gagacatctc atcaaatccc ccaacaagaa gattcatgtc
      901 atttctctct ttcaacataa gcatagcatc tgtgagagca gattcaaatg atattcctcc
      961 atgaacatat gaaacattat aacaatttat tcccaaagaa agtgctatct gggcaccgat
     1021 tgtattgggt gtactctgaa taaatgcaga tggatttaaa agtcgctcat cattttcata
     1081 aatagaattt aaaaatcttt cagtatctgc cagacagccc catccagtcc ccgttattat
     1141 gccatcgatg ctctccaacc cgctatcctt taatgcaatc attgcagaag ttaaacccat
     1201 tttaataatt ctactcattc tgcgtctcgc caaaggatca ggaatataat ctttataatc
     1261 gggttcattc ttaccctcca ctgtagcaca attaaggata tatattctct tgttcatcat
     1321 gtttgtcacc tgttgcaata accaaagata agggttgtgc agttacctcc aaacccaaaa
     1381 gaatttttaa gaacatattt taaatccata ccagatacaa gttttactac aggcataaca
     1441 tcaacatctt tcattcgtac accgaaatta agatttggcc aaacgtatcc atatttcaaa
     1501 gacaaaagag aaaaaacact ttcaacacca cctactgcag ccagagtatg tcctgtataa
     1561 cttttagtag aactgaataa tggcacatta tttccaaaaa tacgcaacaa agcagaacct
     1621 tcagaggcat cattgttagg agttccggta ccatgagcat gaactatgct tatgtcatcc
     1681 ggggaaaggc cacttacctc aagtgcacct gacatagcca aataagcacc tgtcccatca
     1741 gaagaagatg ctgtttgatg atatgcatca tttgcatttg cataaccaca tacctcccca
     1801 tacgtttttt catgtggaat atcagatctt tgaataatca gatatcctgc tccttcacca
     1861 atattcagtc ctgctcttgt ttcatcaaaa gggcggcatt gttctttgtc cagtataccc
     1921 aaagaataga aaccattaac agtaaatctc gataatgaat ctgtacctcc aacaacagca
     1981 acatcaagca gcccattctt aatcattctt gttcccaaca tgattgcatt actggcagat
     2041 gaacacgcag tgcttacagt tgaaacaaag tcatctgcct taaggtagtc cgctattatt
     2101 ttagttgaag acccacaatc atgtccaata agatctctca gtctaccttt acttttatcc
     2161 agacgatatt ctctgtaaaa gttttcactt acatccattc cgcctacaga agttgaagaa
     2221 atcaccccaa tcttcaatct catcctttct tgttggccga gaaaaagaaa acccgagtca
     2281 ctatatgctt ctcgtgcagc aatcagtcca agaagtgcag ttctggaata tcgctttgaa
     2341 gaatcaagac aaagcatttc tatcatttcc atattgccca atctgacctc tccaaccatc
     2401 acatcatggt ttgtttgaaa aagagtaata ggccctatac cactctttaa tgtcatgaga
     2461 gaatcaagat tttcgtttac ccctactccc aaggcggaaa taacccctaa tccactgact
     2521 ataaccttat tgtccatcat acatctttag tatataaagc aatattcaga tcaaaatgtt
     2581 ctccaagcaa ttcacaccaa cccgcaatca ccaaatccat actggataat gaggcatctc
     2641 tgagcaatag cgattccaca tcaaatttcc tttttggatc atggacaaaa aatgtgtttt
     2701 ctcccttaaa tctatttttt atagatatct cacctaaaac cgtattagga agagtgtaga
     2761 caaaaattgc cggactcgcc agtaaatcgt tttctctgcc aattgcagcc atgtgtttta
     2821 gatcagacgc aagtgatgaa gaagaattag caaaaaaaag agctctcttt tcaggaggaa
     2881 aattatcaat ctccggaaaa tttctcatta aagcaatggc accaatatat cctaatttgg
     2941 agagatcatc catcttaaag aatttaatat ccttgttgtc aagacttcta ttccaatccc
     3001 tgataatgtc atgaaaacca gcatcgccac taagcctgaa tcttgaaatt tctttccatg
     3061 aagaggaata aattgaatta acgctttttg aaacagagtc aaaagaataa atgacagcgg
     3121 cattacatcc acca
//
LOCUS       DAOTQL010000160         3074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_24548_length_3074_cov_4.156012, whole genome shotgun sequence.
ACCESSION   DAOTQL010000160 DAOTQL010000000
VERSION     DAOTQL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24548_length_3074_cov_4.156012"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..608
                     /gene="hisIE"
                     /locus_tag="P5293_07905"
     CDS             3..608
                     /gene="hisIE"
                     /locus_tag="P5293_07905"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HRR49857.1"
                     /translation="MKDFNELNFEKNENGLIPAIIQDSKTLRVLMLGYMSKGSLAKTL
                     ETGLVTFFSRSKKRLWTKGESSGNFLKVVSIEADCDGDTILIKVDPKGPVCHKGTLSC
                     FGEENSEGFIRKLSGIIEDRHMQMTTGSYTVKLLSEGRAAIAQKIGEEAVETVIEIIK
                     GDKDRIIYEMSDLIYHLLVLLENEKISINEIEDELFNRHKI"
     gene            712..1029
                     /locus_tag="P5293_07910"
     CDS             712..1029
                     /locus_tag="P5293_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRR49858.1"
                     /translation="MARLEIDGKVFEVDGDGFLTNPEVWNEEVAKLFARNDGIEELTD
                     KHWAVINEIRKNFEEKGNAPMVRTICQNTGLKLREIYELFPLGPARGACRIAGLPKPD
                     GCV"
     gene            1043..1660
                     /locus_tag="P5293_07915"
     CDS             1043..1660
                     /locus_tag="P5293_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49859.1"
                     /translation="MDCYGCCALVACSICFFIFLYRFIYLVRLGNPKDLSQKRGNTSR
                     AIKYSFTGAMLPQNKESAYLHLPTYIAGVLFHIGTFLTLFIFVCQVVFSFVDITIPQI
                     VRQIVAVILIPAIISGFSVLFKRVFSKKLKIISGPDDYISNLLTTSAQLFTAICLFFP
                     DTAPAYYIVMTLFFLWLPFGKTRHLLYFFFARYHLGYFYGWRGTW"
     gene            1654..2955
                     /locus_tag="P5293_07920"
     CDS             1654..2955
                     /locus_tag="P5293_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRR49860.1"
                     /translation="MVTLDKTKKDRAIDILRAESDSKLITHLNSCVRCGLCAESCMYY
                     LALKEPKFIPSVKVNLVSSIYRRYCTFEGKLAPALVNAKELDENMAEEMIDVLYGGCT
                     MCGRCVKHCSIGVDIPFLVHKGREMLATMGLVPLTLQSTVDAAVNTGNNMAIPTDEFV
                     GTLKWLEEDLKDEMQDENARIPIDEENKEIFYTLNPREPKFFPLSISAMAKIFYAANS
                     EWTLSSKYYDVTNYGYFSGNNEEAAIIARHLYDEIHRLHGKRLVLGECGHGSRANRWE
                     GPNYLKKEYDFDTITVVELIAEYIRGEKIKLDKSLNREIFTIHDPCNLVRNGGLMEEI
                     RFVVNSAVDNLIEMTPYGKDNFCCGGGGGALAMSEYNERRLKIGKIKADQIANTGAKV
                     VITPCHNCVDQLMQINHTYKLGVKIMTISEVVANALIIERQ"
BASE COUNT          975 a          479 c          665 g          955 t
ORIGIN      
        1 ttatgaaaga ttttaatgaa ctaaattttg agaaaaacga aaatggtctt attcctgcaa
       61 taattcagga cagtaaaacg ttaagagtcc tgatgttggg atatatgagc aaaggatctt
      121 tggccaagac tcttgaaaca ggacttgtta catttttcag tagaagcaaa aaacgattgt
      181 ggacaaaagg agaaagtagc ggtaactttc tcaaggtggt ttctatagaa gctgattgcg
      241 atggtgatac aatccttatc aaagtggatc caaaagggcc tgtttgtcac aaaggcactc
      301 ttagttgctt tggggaggag aatagcgagg gttttattcg caagctgtca ggaattattg
      361 aggaccgtca tatgcaaatg actacgggtt cctatactgt taagcttctt tctgaaggta
      421 gagcagctat tgcacaaaaa ataggagaag aggcagttga aactgttatt gagattataa
      481 aaggtgacaa agacaggata atttacgaaa tgtcggatct tatatatcat ttacttgttt
      541 tgcttgaaaa tgagaagata tcaataaatg aaattgagga tgaacttttc aatagacaca
      601 aaatataggc aggtataacg tttgcgtaat aatttgtaat aaaaaagtgc aataattgta
      661 aaattatttt ctattttcgt gcatctgata atttaatcaa ataaagcata tatggcacga
      721 ttggaaattg atggtaaagt atttgaagta gacggagatg gatttcttac taaccctgag
      781 gtctggaatg aagaagtagc aaaacttttt gccagaaatg atggcattga agaattgacc
      841 gacaagcatt gggcggttat aaacgaaatt agaaaaaatt ttgaggagaa gggaaatgca
      901 cctatggtcc gtactatttg ccagaatacg gggcttaaac ttcgtgagat atatgaactt
      961 tttccgttag gtcctgcaag aggtgcctgt agaatagcag gccttcctaa accagacggt
     1021 tgtgtatagg agatagcttc ttatggactg ttatggttgt tgtgccttag ttgcatgcag
     1081 tatatgtttt ttcatttttc tctatagatt tatttatctt gtcagacttg gtaatcctaa
     1141 agacctgtct caaaaaagag gtaatacttc aagagcaata aagtattcgt ttacaggagc
     1201 catgttacct caaaacaagg agtctgccta tcttcatctt ccaacatata ttgcaggtgt
     1261 tttatttcat attggaacgt ttcttactct ttttatattt gtctgtcagg tagtattttc
     1321 ttttgtcgat atcacaattc cgcaaattgt acggcaaatt gtggcagtta ttttgattcc
     1381 tgccattatt tcaggttttt ctgtactttt taagagagtc tttagcaaga agctaaagat
     1441 aatatctggt cctgatgatt atatatcaaa ccttctgaca acatctgccc agctttttac
     1501 tgcaatttgt ctttttttcc cagacacggc tcctgcttac tatatagtta tgacactatt
     1561 ttttctatgg cttccgtttg gtaagaccag gcatcttctt tatttctttt ttgcaaggta
     1621 tcaccttgga tatttttacg gttggagggg aacatggtaa cattagataa aactaagaag
     1681 gatagggcta tagacattct tcgggctgaa tccgacagta agttgataac gcaccttaat
     1741 tcctgtgtac gttgtggttt gtgtgcagag agctgtatgt attatttggc attgaaagag
     1801 ccaaaattta ttccgtcggt caaagtaaat ttggtcagtt ctatctacag aagatattgc
     1861 acttttgaag gcaagttagc tccggctctt gtaaacgcaa aagagcttga tgagaatatg
     1921 gcagaagaga tgatcgatgt gctttatgga ggatgtacaa tgtgtggaag atgtgtaaag
     1981 cattgttcaa taggagtaga tataccgttt ttggttcata aaggcagaga gatgcttgcc
     2041 accatggggc ttgttccgtt aactcttcaa tctactgtag atgccgctgt aaataccggt
     2101 aacaatatgg caattcccac ggacgaattt gtaggtactc tcaagtggct ggaagaggat
     2161 cttaaagacg agatgcaaga tgaaaatgca agaattccta ttgatgaaga gaataaagaa
     2221 attttctata ctctcaatcc tagggaacct aaatttttcc ctctctctat ttcagctatg
     2281 gcaaagattt tttatgctgc caattcagaa tggactctat catccaagta ttatgatgtg
     2341 acaaattatg gctatttcag cggcaataac gaagaagctg caattattgc acgacatctt
     2401 tatgacgaga tacacagatt acacggcaaa aggttggttt tgggtgagtg tggtcacgga
     2461 tcaagggcaa acagatggga aggtcctaat tacctcaaga aagaatatga ttttgacacg
     2521 attactgtgg ttgaacttat tgctgaatac ataaggggtg aaaaaatcaa gttggataaa
     2581 agtcttaaca gggaaatatt tacaattcac gacccatgca atcttgttcg taatggaggt
     2641 ttaatggagg agataagatt tgtggtaaat agtgctgttg acaatttgat cgaaatgact
     2701 ccctacggca aagataattt ttgttgtgga ggtggtggtg gagcacttgc catgagtgag
     2761 tataacgaaa gaagattaaa aataggtaag ataaaagccg atcagatagc caatacaggg
     2821 gcaaaagttg taataacacc ctgtcacaac tgcgtagatc aactgatgca aatcaatcat
     2881 acatataagc ttggagtcaa aattatgacc atttctgaag ttgtggccaa tgcattgata
     2941 atagaaagac aataaaataa atgttatgag taaacttatt tatctcgaca actcggcaac
     3001 cagctttcca aagcctgacg ttgtctacga tttcatgaac tctttttacc gaactaaagg
     3061 ggttagtccc ggaa
//
LOCUS       DAOTQL010000161         3063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_24685_length_3063_cov_3.936835, whole genome shotgun sequence.
ACCESSION   DAOTQL010000161 DAOTQL010000000
VERSION     DAOTQL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24685_length_3063_cov_3.936835"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="P5293_07925"
     CDS             <1..1360
                     /locus_tag="P5293_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRR49861.1"
                     /translation="ALTEMTPQQVIDLIKKSGLRGRGGGGFPTGLKWEIAASNKADQK
                     YVVCNADEGDPGAFMDRSILEGDPHSVIEAMAICGYCIGASKGIVYIRAEYPLAIQRL
                     KVAARQCRAYGLLGNDILGTGFNFDIELRYGAGAFVCGEETALIQSMEGKRGEPTVKP
                     PFPAQSGYLRKPTNVNNVETFANIPAILLKGPEWFSSIGTEKSKGTKVFALAGKINNV
                     GLIEVPMGTTLREVIFEIGGGIKGGKKFKAVQTGGPSGGCLTEKHLDTPIDFDNLIAA
                     GSMMGSGGMIVMDEDDCMVSVAKFYLEFTVDESCGKCAPCRVGNKRLHEMLEKICSGK
                     GTMEDVEYLKNLGNVIKDTALCGLGQTSPNPVLSTIEHFYDEYKAHVTERICPAGQCK
                     ALKLYTILADKCTGCTACARSCPVGAISGEKRQAHVIDAEKCIRCGACYDKCKFSAIY
                     VQ"
     gene            1373..>3063
                     /locus_tag="P5293_07930"
     CDS             1373..>3063
                     /locus_tag="P5293_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HRR49862.1"
                     /translation="MEKIKITIDKKEIEVAKGTTIYQAAKDNGIHIPTLCYLNLDHLK
                     IENRPGGCRVCVVEVVGRKTLAPSCSTECTNGMVINTHSVRVLNARKTVLELILSDHP
                     KDCLICSSSGQCDLQKLSQTLGIREIHSVENAAVSTYRKDVSPALKRDMDKCIMCRRC
                     ETICNTVQTVGALSAINRGFMSVVAPAFEQDLIDSPCTFCGQCVAVCPTGALTPFDDT
                     GKVIRALSDPKKTVIVQTAPAVRAALGEEFGLEPGTLVTGKMVAALRSLGFDKVFDTD
                     FAADLTIMEEGTELLTRLSKFLEGDKSVKLPILTSCCPAWVNFFENNYQDMLDVPSTA
                     RSPQQMFGSIAKNYYTQLLGVKREDLVVVSVMPCLAKKYECQRDEFKTNGNPDVDFSI
                     STRELASLIKSANIDFNALDDEEFDAPLGESTGAGVIFGVTGGVIEAAVRTAYELYTK
                     KKLERIDFEELRGLEGIRTATIDFNGTKINIGIAHQLGNARKLLDGIRAGLYNFHAIE
                     IMACPGGCIGGAGQPLHHGNSEIIKARFGAIYREDASKTIRKSHENPYIIKLYEE"
BASE COUNT          869 a          522 c          759 g          913 t
ORIGIN      
        1 ggctcttaca gaaatgactc cgcaacaggt aatagacctg attaaaaaat ccggcttgag
       61 aggaaggggt ggcggaggat ttcctacagg tcttaagtgg gaaattgcgg cttcaaacaa
      121 ggctgatcaa aaatatgtcg tatgtaatgc agacgaaggt gaccctggtg cctttatgga
      181 tcgttcgatt cttgaagggg atccgcattc agtgattgag gcaatggcta tttgcggtta
      241 ctgtataggc gcttcaaagg gcatcgtgta tattcgagct gaataccctc tcgctattca
      301 aaggcttaaa gttgctgcaa gacagtgtcg tgcttatggt cttctcggca atgatattct
      361 tgggactggt ttcaattttg atattgagtt gagatatggt gccggggcat ttgtttgtgg
      421 agaagagacg gctcttattc agtcaatgga gggcaaaaga ggagaaccta ccgttaaacc
      481 tcctttccct gctcaaagtg gttatcttag gaaaccgacc aatgtaaata atgttgaaac
      541 ttttgccaat atccctgcta ttcttttaaa aggccctgaa tggttctcaa gtattggaac
      601 agagaaatcc aaaggtacca aagtgtttgc tcttgccggc aagattaaca atgttgggct
      661 gattgaagtg ccgatgggta caactcttcg tgaggttatt tttgagatag gtggtggtat
      721 aaagggaggt aagaaattta aggctgttca gacaggaggt ccttctggtg ggtgtcttac
      781 tgaaaagcat ctcgatactc ctatagattt tgataatttg attgctgccg gatccatgat
      841 gggttcaggc ggaatgattg tgatggacga agacgactgt atggtctcag tggcaaaatt
      901 ctatcttgaa tttactgttg acgaatcttg tggcaaatgt gctccgtgtc gtgtggggaa
      961 caagagactg catgagatgc ttgagaaaat ctgcagtggc aagggaacaa tggaggatgt
     1021 ggagtatctt aaaaatcttg gaaatgttat taaagacaca gctctttgtg ggctgggaca
     1081 aacttcacct aatcctgtgc tttcgactat tgagcatttc tatgacgagt acaaagcgca
     1141 tgtgactgaa agaatttgtc ctgccggcca atgcaaggct cttaagttat atactattct
     1201 tgcagataag tgtacaggtt gtactgcatg tgcgaggtcc tgtcctgttg gagccatctc
     1261 cggtgagaaa cgacaggcac atgtgattga tgcagaaaaa tgcatccgtt gtggagcttg
     1321 ctatgacaag tgtaagtttt cagcaattta tgttcaataa tgttctctct ttatggaaaa
     1381 aattaaaatt actatagata agaaagagat agaagttgcc aaaggtacga ctatctacca
     1441 ggctgcaaaa gataacggaa ttcatatacc tacgctctgt tatcttaatc tggatcactt
     1501 gaaaattgaa aacagacccg gcgggtgcag ggtatgtgtt gttgaggtag ttggaagaaa
     1561 gactcttgca ccttcttgtt caacagagtg tacaaacggt atggtaataa atactcactc
     1621 tgtgagagtt ctcaatgcaa gaaaaaccgt gctcgaactg attctttcgg atcatcctaa
     1681 agattgtctt atttgttcat cttcaggaca gtgcgatctg cagaagttgt ctcagacact
     1741 cggaattcgt gaaattcatt ctgttgaaaa cgctgctgtt tctacttata gaaaggatgt
     1801 gtctcctgcc ttgaagaggg atatggacaa gtgtataatg tgtcggaggt gtgagacaat
     1861 ttgtaataca gttcagactg ttggagcact tagtgcaatt aacaggggat ttatgtcagt
     1921 tgttgctccc gcttttgaac aggatcttat tgattctcct tgtacttttt gcggtcaatg
     1981 tgtagctgtt tgtccaacag gggctttgac tccatttgat gatactggaa aggtcataag
     2041 agctctttca gatcctaaaa aaacggttat tgttcaaact gcaccggctg ttcgtgctgc
     2101 cttgggtgaa gagtttggtc tcgagccggg aacccttgtg acaggtaaaa tggtggcagc
     2161 tctcagaagt ttgggttttg acaaggtatt tgatactgat tttgctgcag atttaactat
     2221 tatggaggaa ggaactgaat tgctgacaag actttccaag ttccttgaag gtgacaagag
     2281 tgttaaactt cctatactga cttcatgttg ccctgcttgg gtgaacttct tcgaaaataa
     2341 ttatcaggat atgctggatg ttccttctac agcaagatca ccacagcaaa tgtttggatc
     2401 aattgcgaag aactattaca ctcaattgct tggtgttaag agggaagatt tggtggttgt
     2461 atctgttatg ccttgccttg ctaaaaaata tgaatgccag agggatgaat ttaagactaa
     2521 tggcaatccg gatgttgact tttcaatttc tacaagagag cttgcaagtc ttattaaatc
     2581 agcaaatata gattttaatg ctcttgatga tgaggaattt gatgctcctt taggggaatc
     2641 aacaggtgcg ggtgttatat tcggagttac cggaggtgta attgaggcag cagttcgtac
     2701 tgcatatgag ctttacacaa agaagaaact tgaaagaata gactttgaag aattgcgtgg
     2761 tctcgaggga ataagaactg caacaattga tttcaacggt acaaagatta atattggaat
     2821 agctcatcag ctgggcaatg cacgtaagct tctcgatggt attcgtgcag gattgtacaa
     2881 ttttcacgca attgagataa tggcatgtcc tggaggatgt attggtggag cagggcaacc
     2941 tcttcatcat ggaaactctg agatcataaa ggcacgtttc ggggcaattt acagagaaga
     3001 tgctagcaag actatcagaa aatctcatga gaatccttat atcataaaac tttatgagga
     3061 att
//
LOCUS       DAOTQL010000162         3051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_24815_length_3051_cov_4.544059, whole genome shotgun sequence.
ACCESSION   DAOTQL010000162 DAOTQL010000000
VERSION     DAOTQL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24815_length_3051_cov_4.544059"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="P5293_07935"
     CDS             <1..821
                     /locus_tag="P5293_07935"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRR49863.1"
                     /translation="QTNFAISTDCSVKGREDARPEGWWYESSLFFNKLYYRGCGERAL
                     ERALKKIGPKKLKSGRYNMIVENTVSSRLIAPIISAINGANIQQNNSFLQNKLGKKVF
                     SPLLEIYDDAHLPESFGARYFDSEGVATQPMKIIDCGTINTYFVNSYYSKKTGLPVTV
                     DSPSVPECYVAGENNCKIIKDLDFLLNKSLNGVLVTGFNGGNSNSSTGDFSFGIEGFY
                     FENGECLFPVSEMNITGNIISLWNSIVEIGNDPRTSNRWLIPSLLFEDVDFNGF"
     gene            883..1413
                     /locus_tag="P5293_07940"
     CDS             883..1413
                     /locus_tag="P5293_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRR49864.1"
                     /translation="MKIETNKVVSLSYTLEVDGDIVETVTSDKPLEFIFGTGYLLPKF
                     EENVSNMSIGDSFDFTLTASEGYGEENPDAIIELPKDIFKINGEIEEGLLTVGNILPM
                     QDSDGNRLQGSIDEIREDVVIMNFNHPFAGADLHFRGNIVGIREATESELLHGLMGEI
                     GTSCGEGGCSGCSGCN"
     gene            complement(1542..3050)
                     /locus_tag="P5293_07945"
     CDS             complement(1542..3050)
                     /locus_tag="P5293_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR49865.1"
                     /translation="MACLLAALVLSTSCEKDYLDTKPTSETATSTIFETTAGAALAVN
                     GLNKLTTMQYLRSQGFNGEGTIKMYYGNYPGNYFSVNLPGWASVINSQYHENITSIYL
                     YYPWYYYYRIIGNANAIILNIDNATGPQSEKDFIKAQALTYRAYCYFMLSQLYCERWK
                     DSNNGASKGLVLRLDASTGDMPLSTLAEVYQQVYADLDQAIALYTSSGLNRTGNYEMN
                     KDVAYAVYARAAITRQDYTKAKTMAANARANYPLMTNAMYKAGFYSPNQEWIWSCWGS
                     SEETLYFYSYFAYIAYNSSASAVRTYPKCISKELFEKIPSTDIRKSLFLDPTGYTYNT
                     STGVASTALKTRAFQLFPNLYSTATVYAYMQFKIAAADLPGVGHLNNFRSAEMYLIGA
                     EAEYFLNNPTGAQNLLIELNKTSGRDPSYTCTKTGQALLDEIKLYRAIELWGEGFDWF
                     DLKRWGDSVQRKNFASGGNFVATLDVTIQPNEANKWTWMIPQRETDYNKAIK"
BASE COUNT          970 a          558 c          604 g          919 t
ORIGIN      
        1 aacagacaaa ttttgcaata agtacagatt gctctgtaaa aggcagagaa gatgccagac
       61 cggaaggatg gtggtatgaa agctccctgt tttttaacaa actttactac cgaggctgtg
      121 gagaaagagc tctcgaaaga gccttaaaaa agataggtcc taagaaatta aaaagcggca
      181 gatataacat gattgtcgaa aatactgtct caagcaggtt gatagctccg atcatcagtg
      241 ctatcaatgg tgcaaatatc cagcaaaata actctttctt acagaacaaa cttggaaaga
      301 aagtcttttc tcctttactt gaaatctatg acgatgctca tctgcctgag tcttttggag
      361 cacgatactt cgattcagaa ggagttgcaa cccaacccat gaaaattatt gattgcggaa
      421 ctataaacac atactttgta aacagctact attcaaaaaa aacagggtta ccagtcacag
      481 ttgattcacc ttcagtgcct gaatgttatg ttgccggaga aaataactgt aaaattatta
      541 aagatctcga ttttttgctt aacaaatctt tgaacggagt gcttgtaacc ggatttaatg
      601 gtggaaattc taacagttct actggagact tttcatttgg aattgaagga ttttactttg
      661 aaaacggaga atgtcttttt cctgtttctg agatgaatat tacagggaac attatttctc
      721 tctggaattc gatagttgag atagggaacg atccaagaac atcaaacagg tggcttattc
      781 catcattact gttcgaagat gtcgatttta acggatttta ataatttttt gtagcataat
      841 ttttgcagtt aaacagaagt ttaaaaataa ttttaattac agatgaaaat tgagacaaat
      901 aaggttgtat cgctttcata tacccttgag gttgacggcg acattgtgga aacggtaaca
      961 tcagacaaac cacttgagtt tatttttggt accggatacc ttctccctaa attcgaagaa
     1021 aatgtatcaa acatgagtat aggagattca tttgatttta ctctgacagc ttctgaaggt
     1081 tatggagaag aaaaccctga tgccataatt gaacttccaa aagatatttt taaaattaat
     1141 ggagaaatcg aagaaggact gttaacagta ggaaatattc ttcctatgca ggattccgat
     1201 ggaaaccgtc ttcaaggttc aatagatgaa attagagaag acgtagtaat aatgaatttt
     1261 aatcatccct tcgctggtgc tgatttacat ttcaggggaa atattgttgg aattcgggaa
     1321 gcgactgaaa gcgaattatt acatggtttg atgggcgaga taggtacatc atgcggcgaa
     1381 ggtggatgct ccggctgcag cggatgtaac taataacagg cttgagaaca ttttgattct
     1441 caagctgatg tttaggtatg tgttctaaat aattggtaaa tgaaaggccg gctaaaattt
     1501 gttagccggc cttggatttt tattatcgat attaattgtt attacttaat tgctttattg
     1561 tagtcagtct ccctttgagg aatcatccat gtccatttat tggcttcatt aggctgaata
     1621 gtaacatcca aagttgcaac aaaattacca cccgaagcaa agttcttacg ctgaacactg
     1681 tcgccccaac gttttaaatc aaaccagtca aaaccttcac cccaaagctc aatagcacga
     1741 tagagtttaa tctcatccaa aagcgcctgt ccggtcttgg tgcatgtata tgaaggatca
     1801 cgcccacttg ttttgttcaa ctcaatcaga aggttttgcg ctcctgtagg attgtttaag
     1861 aagtattccg cctcagctcc tataagatac atctcagcag aacggaaatt attcaaatgt
     1921 ccaactccag gaagatcagc cgcagcaatt ttaaattgca tatatgcata cacagtggca
     1981 gttgaataaa gattcgggaa gagttggaaa gccctggttt taagagctgt acttgccaca
     2041 cctgttgagg tattatatgt ataacctgta ggatcgagga acagactctt gcgaatatct
     2101 gtagacggga tcttttcaaa caattctttg cttatacact taggataagt tcttacggca
     2161 cttgcactcg aattgtaagc aatataagca aagtaagagt agaagtatag agtctcttct
     2221 gaagaacccc agcaactcca tatccattct tggtttggtg aataaaaacc tgccttgtac
     2281 atggcattag tcataagagg ataatttgca cgggcattag cagccattgt ctttgcttta
     2341 gtataatcct gacgagtaat agcagcacgt gcatatacag cataagccac atccttgttc
     2401 atctcataat tacctgttct gttcaaacct gaagatgtgt acaaagctat tgcctgatca
     2461 agatctgcat aaacctgctg ataaacctct gccaatgttg aaagtggcat atcacctgta
     2521 gaagcatcga gacgaagtac caatcctttg gatgcaccat tgttggaatc tttccagcgt
     2581 tcacaataca actgtgatag catgaaatag caataagccc tgtatgtaag agcctgagcc
     2641 ttaataaaat ctttctcgct ttgaggtcct gttgcattat ctatgtttaa gataatggca
     2701 tttgcattgc caattattct ataataataa taccatgggt aatacaaata gatagaagta
     2761 atattctcgt gatattgtga gttaatcaca gaagcccatc caggaagatt tacagaaaag
     2821 taattacccg gataatttcc atagtacatt ttaatagttc cttcgccatt gaaaccttga
     2881 gaacgaagat attgcattgt tgtaagcttg ttaagcccat tcactgcaag agctgcgcct
     2941 gctgttgtct caaatatcgt agaggtagcg gtttcagaag ttggctttgt gtccaaataa
     3001 tctttttcac aacttgtaga caaaaccagt gctgccagca agcatgctat t
//
LOCUS       DAOTQL010000163         3027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25070_length_3027_cov_3.664872, whole genome shotgun sequence.
ACCESSION   DAOTQL010000163 DAOTQL010000000
VERSION     DAOTQL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25070_length_3027_cov_3.664872"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="P5293_07950"
     CDS             <1..271
                     /locus_tag="P5293_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49866.1"
                     /translation="EVKKYKKSFIKYSIDDIDFNEIRNLLCELQGADNKADVWRYLMN
                     KECDLYFTDSKFVDYLNIKVVIGDLYNIFDFLYHITSEYLSGDPE"
     gene            734..961
                     /locus_tag="P5293_07955"
     CDS             734..961
                     /locus_tag="P5293_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49867.1"
                     /translation="MFAVSSTDHDKAKVSILIYADTKRCYKVYDYKKAFKFEPNKMYC
                     ISGKVNSADKLYLILENAREDKLYSLSKVHI"
     gene            complement(972..2153)
                     /locus_tag="P5293_07960"
                     /pseudo
     CDS             complement(972..2153)
                     /locus_tag="P5293_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(2440..>3027)
                     /locus_tag="P5293_07965"
     CDS             complement(2440..>3027)
                     /locus_tag="P5293_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR49868.1"
                     /translation="ALLKTGALFDPVLAATIRDQTGKSALVAGNAFTHHSWSNLAVHE
                     STLANKEFELVALPQAPTGDKPGMYLKPSMFFSITKTSPNKEMAAKFIDFFINDTEAN
                     IILAAERGIPVSSKVRPEVAKTLDGIAKKTLDYVNSVSGMVSPIDPPEPKGADSVMKL
                     ISEVYNELLAGKITPAEAADKVFTGSNEILAKNKK"
BASE COUNT          891 a          583 c          502 g         1051 t
ORIGIN      
        1 tgaagtaaaa aagtataaga agtcgtttat caaatattca atagatgata ttgattttaa
       61 tgagattcga aatcttttat gcgaattaca aggagcagat aataaagctg atgtctggag
      121 atatttaatg aataaagaat gtgacctata ttttacagat agcaagtttg tagattactt
      181 aaatataaag gttgtaattg gagatttgta taatattttt gattttttat accacattac
      241 ttcagagtac ttatcaggtg atccggaata aactttatat taatttgtgc aacgaagtac
      301 tatattgtga cggtataccg tcctttagat tctctgaaca aggagtttat gcttgtgtta
      361 ttctaaaagt ggttctttcc aaagttagtt gaataacttt attttttcaa ggggaatatc
      421 agaaatagtg tgtaaattaa ttttgcttta gaaattcaac gttgatattt ctatattaat
      481 aatattgcca ttaacgtggt aaattataca ttaaaaataa cattcgttcg ggaaatatac
      541 gaataatttg cattcttttg gcaacaataa taaatgaggc ccggctatgc ccgataacct
      601 gctatgccgg gaagcaacag gaaacctgca accgggctga agtatatact tccaggaagg
      661 taataaatcc tacataggat tctatattat ataccttgtg gtgatgccca tgtttaactt
      721 tagtgcaaac catatgtttg ctgtatcttc tactgatcat gataaggcca aagtgtcaat
      781 actgatctat gcagatacaa agaggtgtta caaagtatat gactacaaaa aggcctttaa
      841 gtttgagccg aataagatgt attgtatttc gggtaaagtc aattcggccg ataaactgta
      901 tctcatactt gagaatgcca gggaagataa actgtattca ctttccaagg tacatatttg
      961 aactgggact tttaaagtcc cagttcaagc cttccttcaa atagtattgc aaattgtgca
     1021 actacttgtc cccaattggc taccggcata gtccactttt taaggatatc cattgttccc
     1081 agatacagag actttttcaa tgcctcgtca ttagaaaaaa cacttttagt ctttgttacc
     1141 ttacggagtt gccggttaaa tccttctaaa gtattagtcg tatatattat ctttctaatc
     1201 tctgggggat atttaaagaa tgtagagagg ttctcccagt tatcatacca agattttatt
     1261 ataatcgaat actttttgcc ccatttgctt tccaaaagct caagattttg aaatccaatc
     1321 tcttctgaag ctgccttgta aactggtttt aagtccttca tgaattcttt cttatctttt
     1381 gaggccacat attttattga attgcaaatt tggtgtataa tacaggtttg tatctctgca
     1441 tcaggaaaaa cactttttat agcctcggga aatcctttta acccatctac ggatgccagt
     1501 aatatgtcct gtacgccacg agccttaagt tcattacaga caccaagcca aaactttgct
     1561 ccttcacttt ctccaatcca tattccaagg atatctttca taccttcatt gttaatccca
     1621 agacatatat atgcggcacg attcactact cttccatcat cacgtacttt aaaatgcatc
     1681 gcgtccatgt aaacaattgg atatacttta tcaagaagcc tgttctgcca atctgcaaca
     1741 ttagttataa ctttgtcggt taccatagat ataaagtccg gtgaaacttc tacgccatat
     1801 acttctctta tttgtgcctg gatatcacgg acagtcaacc cctttgcata catgcctata
     1861 attttcttat caaattcacc acacgtactt tgatagtttt caataaccat tggcctaaag
     1921 ccactttgcc tatcactcgg aatattaaca tcaaattctc ccacactact ttttacagtt
     1981 tttttgcggt atccattcct atattctggc tcatctgtat tagttctttc atatttttta
     2041 cgtcccatat actcttcgat ctcggcttcc atcatttttt caattgcatc cttaatgagt
     2101 tttttcagta acccattttc accaaaaaga tcatcctgag tcttacattt tttatttctt
     2161 cttcataatt aatgatattt cccgtatttc cgcttgcttt tactgtactc atatttccca
     2221 tcctcctaaa aaaactgttt tttgaattct tgccaatttc ctaacagtat tttcagagtt
     2281 taatgaaaaa actgccagaa agagttttta ttttacacac tcgttctgac agtctctaca
     2341 tatatttgta taacaagctc ggacaaggta gggccgataa agtttgagac tgtcaaaaag
     2401 agtttttaca cactcattct gacagtctcg cagtaaaaac tatttcttgt ttttggcaag
     2461 aatctcattt gagcctgtaa acactttgtc agctgcttct gcaggggtta tttttccggc
     2521 taacagctca ttgtagacct cactgataag tttcatgacc gagtcagcac ctttaggctc
     2581 cggcgggtca ataggagaga ccattccgga tacactgttt acatagtcca aagtcttttt
     2641 tgcaataccg tcaagagttt ttgcaacttc cggcctgact tttgatgaaa ctggtatgcc
     2701 acgttctgcg gcaagtataa tattggcttc agtatcattg atgaaaaagt ctatgaattt
     2761 ggctgccatt tccttgttag gagatgtttt tgtaattgag aaaaacatcg acggtttcaa
     2821 atacattcca ggtttgtctc cggtaggagc ttgcggtaat gctacaagtt caaattcttt
     2881 attggccaaa gtggattcgt gtactgcaag attactccat gaatggtggg taaatgcatt
     2941 acctgcaacc aaagcacttt tacctgtttg gtctcttatt gtggcagcca aaacaggatc
     3001 aaacagtgca ccggttttta ataatgc
//
LOCUS       DAOTQL010000164         3023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25115_length_3023_cov_4.413747, whole genome shotgun sequence.
ACCESSION   DAOTQL010000164 DAOTQL010000000
VERSION     DAOTQL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25115_length_3023_cov_4.413747"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..548)
                     /locus_tag="P5293_07970"
     CDS             complement(126..548)
                     /locus_tag="P5293_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49869.1"
                     /translation="MFPEKMTNFIEEHHVLTLAVTDGDDLWCSHAFYTFVEAEQLFVI
                     TSEDKTRHTTLIERGSNIVAGAIALETEKIGLIRGLQFKAEAIKCDRSLFNKYRFMYL
                     KRFPYAILTSSDIWILRIIEAKFTDNRLVFGKKLIWKR"
     gene            complement(538..>1509)
                     /locus_tag="P5293_07975"
     CDS             complement(538..>1509)
                     /locus_tag="P5293_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRR49870.1"
                     /translation="AIIASDMAVIQYARKVGMEVHISTQLNVSNIESVRYHSQFADVI
                     VLARELTLTQIKNIGEAIRREQIKGPSGRLVALELFCHGALCMAVSGKCYLSLHQYNA
                     SANRGACYQICRRGYQVIDLETGMQLEIDNKYIMSPKDLCTIEFVDEILDAGVTVLKI
                     EGRARSAEYVKYVTSAYRSAVDAVCNGTYSKEMAAELKQRLSTVFNRGFWDGYYQGAK
                     IGEWSNVYGNKATRRKRYVGKVTNYFSNLSVAEVLIETGELCQGDTVLISGPSTGVIE
                     CNVEEIRVDLQVAVKAVKGDYCSIPVPGGVKLRRSDKVYIWEKSDVS"
     assembly_gap    1510..1609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1610..1952)
                     /locus_tag="P5293_07980"
     CDS             complement(<1610..1952)
                     /locus_tag="P5293_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRR49871.1"
                     /translation="MNIKREDIEIMSPAGNFEALQAAIQGGADSVYFGVGELNMRSHS
                     ANNFLIDDLPKIVEICRGNGVKSYLTLNIIIYDNELGQMRQTVDAACRAGVSAIIASD
                     MAVIQYARKVGM"
     gene            complement(1952..2971)
                     /locus_tag="P5293_07985"
     CDS             complement(1952..2971)
                     /locus_tag="P5293_07985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRR49872.1"
                     /translation="MGKRTRHIILLRFSAIGDVAIGAPLIRYYAGANPDIKFTIVSQP
                     RLEPLFSGIDNLYFFPVDFKKKHKGFKGLTRLFLELSRLGPTDIADLHNVTRTWVLRS
                     YFFFTFKRIAFIHKGRFEKAKLTRKRNKVLKQLPSTISRYEKVLVKLGLKDLNFSGRV
                     NKPFSVKRVNYFKIGIAPFAKHKGKSWPFEYIEEVVAALSKEGHYSIFLFGGGKVEGN
                     MLHALEQRYSGVHSVAGKYSLREELEIIKDLDMMLSMDSANMHLASFVGVPVISIWGA
                     THPYTGFYGWNQSPDNAVQIEMECRPCSVFGNKKCFRGDYACLLNIKPQLVLNKIEEL
                     TSGEV"
BASE COUNT          864 a          656 c          482 g          921 t
ORIGIN      
        1 accaaagtag tggaaattat agaaaacgga gtgcctgtgc gtaaggagat acaattcttt
       61 ggatgggaaa aacttgattc agtcgaaatg attaagaagg agtttggaat tatctaacga
      121 tccctttaac gtttccatat taattttttg ccgaaaacca gacgattgtc agtaaatttt
      181 gcctcaatga ttctcaaaat ccagatatca ctgcttgtaa gtatagcata gggaaatctt
      241 tttaaataca taaatcggta tttattaaaa agagaacggt cacattttat tgcttcagct
      301 ttgaattgta aacctcgaat aagacctatt ttttcagtct caagagctat tgctcctgct
      361 actatgttgc tccctctttc aatcagagtt gtgtgtcttg tcttatcttc tgaagtaata
      421 acaaaaagtt gctctgcttc aacaaatgtg taaaaggcat gagagcacca taaatcgtct
      481 ccgtcagtta cagcaagtgt gaggacatga tgctcctcaa tgaagtttgt cattttttca
      541 ggaaacatct gatttttccc aaatatatac tttatccgat cgtctaagct ttactcctcc
      601 tggtacaggt atagaacagt aatcaccttt gacagccttt acagcaactt gaagatcgac
      661 tcttatctcc tcaacattgc attcaatcac tcccgtggaa ggaccggaaa taagaacggt
      721 atctccttga cacagttctc ctgtctcaat caaaacttcg gcaactgaaa ggttggagaa
      781 ataatttgtt actttaccaa catacctttt tcttcgagtt gctttgttac catatacatt
      841 gctccattct ccaattttag ctccctgata ataaccatcc caaaaacctc tgttaaagac
      901 tgttgaaagt ctctgtttaa gttctgctgc catttccttg ctgtatgtgc cattacatac
      961 tgcatccacg gcgcttctat aggcagaggt gacatactta acatattcag ccgatcttgc
     1021 tcttccttca attttcaata cagtcacgcc cgcatctaaa atttcgtcaa caaattctat
     1081 tgtacaaaga tccttggggg acattatata tttgttgtct atctccagct gcatccctgt
     1141 ttcaagatca attacctgat aacctcttct gcaaatttga tagcaggcac ctctattagc
     1201 agaagcatta tattgatgaa gactgagata acactttcct gaaacagcca tacaaagagc
     1261 tccgtgacaa aaaagctcta aggctacaag tcttcctgaa ggtcctttaa tctgctctcg
     1321 ccttatagcc tctccaatat tcttaatttg agtaagggtc aactctcttg ctaaaacaat
     1381 aacatcggca aactgtgagt gatatcttac gctctcaata tttgaaacat tcagctgagt
     1441 agaaatgtga acctccatgc caacttttct tgcatactga atgacggcca tgtcagaagc
     1501 tatgatggcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc catgccaact
     1621 tttcttgcat actgaatgac ggccatgtca gaagctatga tggccgaaac ccctgctctg
     1681 caggcagcat ctacagtctg tcgcatctgt cctaactcat tgtcgtatat tattatgttt
     1741 agagtcaaat atgatttaac tccatttccc ctgcatattt caacaatctt tggcaaatca
     1801 tctatcagaa aattatttgc agaatgagac cgcatattca attctccaac tccaaaatat
     1861 acagaatcgg ctcctccctg tatcgccgcc tgcagagctt caaagttgcc tgccggtgac
     1921 ataatctcta tatcctctct tttaatattc atcaaacctc tccagatgtg agttcttcaa
     1981 tcttattcaa cactaattgt ggtttaatgt tcaacagaca tgcatagtcc cccctgaagc
     2041 attttttatt gccaaaaaca gaacaaggtc tacactccat ctctatctgg acagcattat
     2101 cagggctctg attccaacca taaaaaccag tatagggatg agttgctccc catattgata
     2161 tcacaggaac accaacaaat gaagctaaat gcatgtttgc agaatccatg gagagcatca
     2221 tgtcaagatc tttaataatt tcgagctctt ctcttaaaga gtacttgccg gcgacagaat
     2281 gtactcctga atatctttgt tccaatgcgt gcagcatgtt cccctcaacc ttgccacctc
     2341 caaatagaaa tatactgtaa tgcccttctt tgctgagagc tgccacgact tcctctatat
     2401 attcaaatgg ccaagacttg cctttatgct ttgcaaaagg agcaattcct attttaaaat
     2461 aatttaccct ttttacagaa aacggcttat ttaccctgcc tgaaaagtta agatctttca
     2521 aaccaagttt tacaagaact ttctcatatc gacttatagt cgatggcaat tgtttcaaca
     2581 ccttgttcct tttccttgtc aactttgctt tttcgaatct tcccttgtga ataaaggcaa
     2641 ttctcttgaa agtaaaaaaa aagtaactcc gaagcaccca tgtgcgtgta acattatgca
     2701 gatctgcgat atctgtaggt cctaacctgg aaagttccag aaaaagtctg gtaagaccct
     2761 taaagccttt atgtttcttt ttaaagtcaa ccggaaagaa gtaaagattg tctattccgg
     2821 aaaacaatgg ttcgagcctg ggttgtgaca caattgtaaa tttaatatct ggattagccc
     2881 cggcataata cctgataagt ggcgctccta tggcaacatc acctatggct gaaaacctta
     2941 ataaaatgat atgcctagtt ctttttccca ctctgataaa gtatcggatt taatgcgggg
     3001 tcattataca tcttcatctg ttt
//
LOCUS       DAOTQL010000165         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25126_length_3022_cov_4.181665, whole genome shotgun sequence.
ACCESSION   DAOTQL010000165 DAOTQL010000000
VERSION     DAOTQL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25126_length_3022_cov_4.181665"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5293_07990"
     CDS             complement(<1..269)
                     /locus_tag="P5293_07990"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRR49873.1"
                     /translation="MMKILEYSQIKGSEIMNSRNREFNDIIQLRVSEIINRVVKGGDK
                     ALRELTKELDGADIDSLFVTNEELLSEGNLSDIELEQALNVAASN"
     gene            complement(266..1117)
                     /gene="hisG"
                     /locus_tag="P5293_07995"
     CDS             complement(266..1117)
                     /gene="hisG"
                     /locus_tag="P5293_07995"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRR49874.1"
                     /translation="MIRLAVQAKGRLNEESVELLQEAGFPVEMSKRKLVAKCGSFPLE
                     ILYLRDDDIPQAVSMGVADAGIVGLNEVKERQFEVGQVHKLSFGNCRLSLAIPKSEEY
                     TGMEYFNGKRIATSYPNILTDFFSRSGVRAEIHQITGSVEIAPSVGLADAIFDIVSSG
                     GTLLSNGLKEVLTVMESQAVVIATPNINGDKKELLDQMIIRFEAVERSKGMKYLLMNL
                     PKDRIEDVTKIFPGMRSPTILPLAQEGWCSLHVVVAESDLWTRLAGLKAIGAEGILVF
                     SLEKMMP"
     gene            complement(1342..2115)
                     /gene="ccsA"
                     /locus_tag="P5293_08000"
     CDS             complement(1342..2115)
                     /gene="ccsA"
                     /locus_tag="P5293_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRR49875.1"
                     /translation="MIWDKFIYFALASIVFWCVAAVFLYFAKKKTATHFLVGAGLIVF
                     FSFIVLLWCENGYPPFRTVGETRLWYSLFLIIVGYIIWLHWGYKWILSYSVMVASVFV
                     IINLLKPEIHSSGLIPALQSFWFVPHVIVYILSYALMGAAVIAAFVALVAGEKKDVEL
                     TTLIDNLVNTGMGLLLMGILMGAVWAKEAWGDYWTWDLKEIWAFITALTYLIYIHLRR
                     MNYNRKISFLVIIIAFVMLMITWLGVGYLPSAGQSIHTY"
     gene            complement(2112..2927)
                     /locus_tag="P5293_08005"
     CDS             complement(2112..2927)
                     /locus_tag="P5293_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRR49876.1"
                     /translation="MKREGIFVMGAIIVGVVLQIVSGPFPMELIKFPVNFILALVLLI
                     LSVKGGKVFRKSVHSLKFSVFLILLLTVQVLIMGFMPDNRIKDSWPFVITYFFILVDL
                     AIVISERLKKKGRNSSSFLLIHAGLLILLIAAGLGSSDKIQLFLKVNEGESVRNGYDR
                     LTGKTYRLPFEITLEDFSIEYYPDRSIKSYNSLVTLKNSDSKAKKWDIRVNHPLRYGS
                     WWIYQYSYDLDKGGDSKYAVLQLVRDPWLILAWVGISILFAGAVNMFWKGGRP"
BASE COUNT          992 a          698 c          448 g          884 t
ORIGIN      
        1 atgtttgagg ctgctacatt caatgcttgc tctaactcta tatcagataa attaccttca
       61 gataaaagct cttcatttgt cacaaaaagt gaatcaatgt ctgctccatc aagctctttc
      121 gttaactccc tcaaggcttt gtcaccacct tttactacac gatttatgat ttctgatact
      181 cgaagctgta taatatcatt gaactctcta tttctcgaat tcattatttc gctcccctta
      241 atttgagaat attctaatat cttcatcatg gcatcatttt ttcgagtgaa aaaacaagaa
      301 taccttctgc accaatggcc ttaagtccag caagccgcgt ccaaagatca ctttcagcca
      361 ctactacatg aagtgagcac catccctcct gagcaagtgg aagaatagtg ggacttctca
      421 tccctggaaa gatttttgta acatcttcta tcctgtcctt tggtaaattc ataagtaagt
      481 atttcatacc cttgctgcgt tcaactgcct caaaacgaat aatcatctgg tcaagaagtt
      541 ctttcttgtc accattaata tttggagtcg cgatgaccac agcttgagat tccatgacag
      601 tcaagacctc tttcaatcca tttgaaagaa gagtgccccc tgagctgaca atgtcaaaaa
      661 tggcatctgc aagtcctact gaaggcgcta tctctactga acctgttatt tgatgaattt
      721 ctgcccttac acctgatctg ctaaaaaaat cagttagaat attgggatag gaagtcgcaa
      781 ttcttttccc gttaaaatat tccattcctg tatattcctc actttttggt atggcaagtg
      841 aaagtctgca atttccaaat gacagcttat gaacctgccc aacttcaaac tgacgctctt
      901 taacctcgtt caaacccacg attccagcgt ctgccactcc catcgataca gcctgaggaa
      961 tatcatcatc cctaagataa agaatttcaa gcgggaatga tccgcatttt gcaacaagtt
     1021 ttcttttact catttctaca gggaaccccg cttcctgcaa cagttccaca ctttcttcat
     1081 ttaaacgtcc ctttgcttgg acagccagtc taatcatgtt cgaatattta tttaaaaaaa
     1141 aagagcttgc caaatttggc aagctctgct ctaattattt atataactat gaatatcttt
     1201 ctatatataa acagaaacaa caacctgcca cctgtgatga caatgatgat gatggatagc
     1261 gttgttgttt gttcttatta acatctcttc attaattgct gcaaaattaa aaaaaatctg
     1321 aattatcaaa aaatattttc attagtatgt atgaatgctt tgacctgcag acggaaggta
     1381 accaactcca agccacgtga tcataagcat cacaaaggca attattatca caagaaaaga
     1441 aattttccga ttatagttca tccttcttag atgaatgtaa atgagatatg tcaatgcagt
     1501 aataaaagcc catatttcct tgagatccca tgtccagtaa tctccccatg cctctttggc
     1561 ccaaacagcc cccatgagta ttcccatcaa aagtaatccc atgcctgtgt taacaagatt
     1621 atcaattagc gtagtaagtt caacatcttt tttttctcct gcgacaaggg caacaaaagc
     1681 agcaattaca gcggctccca taagtgcgta agaaagaata tagacaatta catgcggcac
     1741 aaaccagaaa ctttgcaaag ccggaataag accggaagag tgaatctccg gtttaagcaa
     1801 attaataatc acaaatacag aagcaaccat aacagaatag ctcaagatcc atttataacc
     1861 ccaatgaagc catataatat acccaacaat tatcagaaat aaagagtacc agagtcttgt
     1921 ttcacctacc gttctaaatg gtggatatcc attttcacac cacaacaaaa caataaatga
     1981 gaaaaataca attaatcctg ctccaacaag aaagtgagtc gctgtcttct tttttgcaaa
     2041 ataaagaaat actgccgcaa cacaccaaaa gacaattgat gcgagagcaa aatatataaa
     2101 tttatcccag atcaaggcct tcctcctttc caaaacatgt taaccgcacc tgcaaaaagt
     2161 atggatatac ctacccatgc taatatcaac cagggatctc gaacaagttg aagcacagca
     2221 tactttgagt cacccccttt atccaagtca tatgaatatt gataaatcca ccaacttcca
     2281 taccttaatg gatgattgac tcttatatcc cattttttag ccttactatc actgtttttc
     2341 agtgtcacca acgaattgta actttttatc gacctgtcag ggtagtactc aatgctgaaa
     2401 tcctccaaag taatttcgaa aggtaaccga tatgttttcc cagttaatct gtcataacca
     2461 ttcctgacag attctccttc attcaccttc aaaaaaagtt ggattttatc tgaacttccc
     2521 aaccctgctg ctatcagcag aatcaataaa ccagcatgaa ttaacaaaaa actcgaactg
     2581 tttcttcctt tttttttcaa acgttctgaa ataacaatcg cgagatcaac caatataaag
     2641 aaataggtga tgacaaaagg ccaggaatct ttaatcctgt tatcaggcat aaaccccatt
     2701 atcaaaacct gtacagtcaa caaaagtata agaaagaccg agaattttag tgaatgaaca
     2761 ctcttgcgaa aaactttccc gccttttacg gaaagaatta ataaaacaag cgcaagaata
     2821 aaatttaccg gaaattttat caattccata gggaaaggcc cggaaactat ttgtaagaca
     2881 actcccacaa taatagctcc cattacaaat atgccttccc tcttcatgcc agcagattta
     2941 gattatctca ccctaagcct ctctccggca tcttctttga ctgctctctt ccatttttca
     3001 gaataacccg gctgcaaagg ag
//
LOCUS       DAOTQL010000166         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25229_length_3012_cov_4.201556, whole genome shotgun sequence.
ACCESSION   DAOTQL010000166 DAOTQL010000000
VERSION     DAOTQL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25229_length_3012_cov_4.201556"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>3012
                     /locus_tag="P5293_08010"
     CDS             246..>3012
                     /locus_tag="P5293_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49877.1"
                     /translation="MKQLTKRIALTAIGLFVAIASFAQVTTASISGFVKDTKGAPIAG
                     AVIKATHNPTGTVYYVSTQNNGQYNISGMRIGSQYQIEASCMGYAAVKITDVTLTLGD
                     ELKFDFSLQEEAVNLGEIVVTAEINPAINRNRTGAQEIFTRERMSQIPTINRSLTDFT
                     KLTPMASGSNFAGVSYRYNNMTVDGASFNNSFGLSSSLGASGIEPISLEAIEQIQVMI
                     APFDVRNGAFTGGAINSVTRSGNNQWNGSAYYYTKSPSTRGYIQKDAIIAVTDFFNRQ
                     YGATVSGPIIKNKLFFFVNAEFDRQEVPISWRPRPNKSTAVSGYYSAADVETLEEIVQ
                     LLKDNYNYNPGTYNVTSTPTEADKVTARFDWNINTKNKLSFKYFYLRSFSTSNPSTSG
                     AMANGRGPNSFAIPFSSAYYRLNNNFNIFIADLNTIVNDRMSNSLTFGYSRLRDFREM
                     DGGFFPEVSIGDGSASKNSLTTFGTEANSYNNKLDSDIYQIQDNFIINLNKHQLTIGT
                     QSDYRMFVNGFANSFAGQWQFASLDDFKSDVLAYKAWKDAGSNPATRPGTKAIYYKQT
                     YSMMDDGRFPYAEVNVMSLGFYAQDKWSLKDNLNITMGLRVDVPLFMTDLPRNEMLEG
                     LTFQGGRKIDVSKYPSAKPLFSPRLGFNWDVFSNKTLQVRGGTGIFSGTPPYVWLSNQ
                     AGNNGILFGSTTVRDHAFDGVTELPKPTGATLPKASIAITDPDFKYPQLWKTNLAVDY
                     KFGKGWIATAEVLYNKDVNSIYHMDINHPDISDATKVTKLGGVDQRPYFIDNTLNDAT
                     YDVILMTNTSKGYSIYTTLQLQRNFTSGPLNGLYLNASYTFGKAKSVTDGSSSVASSA
                     YKYRPAINPDAEELGFSAGSFPQRILVQGSYRREWGPNFATSIGFVYQMYMPFRYSYT
                     YNGDVNND"
BASE COUNT          941 a          590 c          595 g          886 t
ORIGIN      
        1 gatttgagtt tgctgaaaat taacgaccga aagctcaatt ttagcatttt ttcacgtggt
       61 tgcaagcagt caaatctttc cacttttatg cctgcaaatt tagtataaat tgaaatagtt
      121 ttatatttat tattaaattt attgcaaaat tgttaaaatg ttaataaaat cattaagtaa
      181 tttacaagtt cttatccctt tttcttaatt ttgctccata ataactataa tttatctaat
      241 catctatgaa acaactaaca aagagaattg ctctgacagc gatagggctg tttgtggcca
      301 ttgcgtcttt cgcacaggtg accactgcaa gcatctccgg atttgtgaag gacactaagg
      361 gtgcacccat tgccggtgct gtcataaagg cgactcacaa tcctacaggg acagtctatt
      421 atgtttccac tcagaataat ggacagtaca acatctctgg catgaggata ggatctcaat
      481 accagataga agcttcatgc atgggatatg cggcagttaa aattactgac gtaaccctga
      541 ctcttggcga cgagcttaag ttcgacttct ctttacagga ggaggctgta aatttgggag
      601 agatcgtcgt tactgcggaa attaatcctg caatcaatag aaacagaacc ggagcgcagg
      661 aaatttttac acgtgaaagg atgtctcaaa ttcctacaat caacagatct ctgacagact
      721 ttaccaaact tacccctatg gcaagtggaa gtaattttgc tggagtttcc tacagataca
      781 acaacatgac cgttgatgga gcatctttca ataactcgtt tggtctttca tcttcacttg
      841 gagcttcagg tatagagccg atttcactcg aggccataga gcagattcag gtgatgatag
      901 ctccatttga cgtgagaaac ggcgctttca caggtggagc tattaactct gtaaccagat
      961 ccggaaacaa tcaatggaat ggctctgcat actattatac aaaaagtcct tcaaccaggg
     1021 gctatattca gaaagatgca attattgcag ttacagattt ttttaaccgt caatacggtg
     1081 caacagtcag tggtccgatt atcaaaaaca aactgttttt ctttgttaat gctgaatttg
     1141 acaggcagga agttcctata agctggaggc cgagaccaaa taaaagcact gctgtttcag
     1201 gatattattc agcagccgat gttgaaacac ttgaggagat tgttcaattg ttaaaggaca
     1261 attataatta taaccccgga acatataatg taaccagtac tccaactgag gcagacaaag
     1321 ttaccgcaag atttgactgg aatatcaata caaagaataa gctaagtttc aaatattttt
     1381 atcttagatc attcagtaca agcaatccga gcacatcagg cgcaatggca aatggacgcg
     1441 gacctaacag ttttgcaata ccattttcaa gcgcgtatta caggctaaac aataacttca
     1501 acatatttat tgctgaccta aacactattg taaacgacag gatgtccaac tctcttactt
     1561 tcggttattc aagacttaga gactttagag agatggatgg tgggtttttc cctgaggtta
     1621 gtatcggaga tggcagcgca tccaaaaatt ctttgacaac attcgggaca gaagcaaact
     1681 cttataataa caaactggac agtgatatat atcagattca ggataacttt atcattaacc
     1741 tgaacaagca tcagttgaca attggtaccc aatcagacta cagaatgttt gtcaatggtt
     1801 ttgcaaacag ttttgccggt caatggcaat ttgcatccct tgatgatttc aagtctgatg
     1861 ttcttgcata caaggcttgg aaagacgcag gttctaatcc tgcaacaaga cccggaacca
     1921 aagcaattta ttacaagcag acctacagta tgatggatga cggcaggttc ccatatgctg
     1981 aagtaaatgt tatgtcactt ggcttttatg ctcaggacaa gtggagcctt aaggataatc
     2041 ttaacataac aatgggtctc agggttgatg ttccgttatt tatgaccgat ctgcctagaa
     2101 atgaaatgct cgagggtctt acattccagg gtggaaggaa aatagacgtt tcaaaatatc
     2161 cttcagctaa gccactcttt tcaccaagac ttggtttcaa ttgggatgtc ttttccaaca
     2221 agactcttca ggtcagaggc ggtacaggca ttttctcagg tactcctcca tatgtatggc
     2281 tttctaatca ggcaggaaac aatggtattc ttttcggttc aaccacagtc agggatcacg
     2341 cgtttgacgg tgtaacagaa cttccaaaac ctacaggagc tactttgcca aaggcctcta
     2401 tagccataac cgatcctgat ttcaaatatc ctcaactttg gaagaccaat ttagctgtgg
     2461 attacaagtt tgggaaagga tggatagcaa ctgcagaagt gctttataat aaggatgtta
     2521 attccatata tcacatggat ataaatcatc ctgacataag tgatgccacc aaggttacaa
     2581 aactgggagg tgttgatcag agaccatatt ttattgataa tacattgaat gatgctacat
     2641 atgatgtcat acttatgaca aatacatcta agggttattc aatttacaca actcttcagt
     2701 tacagcgaaa tttcacaagc ggaccactta atggtttgta tctaaatgcc tcatacacat
     2761 ttggcaaagc aaagagtgtg acagacggtt cctcttcagt cgcctcttca gcttacaaat
     2821 atcgtcccgc catcaatcct gatgctgaag agttgggatt ttctgcaggt tcgttcccac
     2881 aaagaattct tgtacaggga tcttacagga gagaatgggg acctaacttt gctacatcaa
     2941 tcggttttgt ttatcaaatg tatatgcctt tcagatattc ttatacatat aatggtgatg
     3001 taaataatga tt
//
LOCUS       DAOTQL010000167         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25359_length_3002_cov_2.949440, whole genome shotgun sequence.
ACCESSION   DAOTQL010000167 DAOTQL010000000
VERSION     DAOTQL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25359_length_3002_cov_2.949440"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..432)
                     /locus_tag="P5293_08015"
     CDS             complement(37..432)
                     /locus_tag="P5293_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49878.1"
                     /translation="MIQFEFSKVERWLKSIVALITILGVVWGVFEFVSSTRQTITMQK
                     QTRDEFVEFRQDIGGKIDTLKNSVDEIKTIQYNQQILLDNFIRSYKDYLSDYAKLNTQ
                     QYRSLTDDMEKMRDDLKKNQYQIPYNTEK"
     gene            complement(442..2052)
                     /locus_tag="P5293_08020"
     CDS             complement(442..2052)
                     /locus_tag="P5293_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRR49879.1"
                     /translation="MRQYYEYTNRSFQYVIDQYGRHLRVGVRNNKFVVDAELTANGFS
                     GVENIGWVNVRKSNYEILGNKFREGIRNNEYKVDAERTYLGFNGIAGIDWEEVGFLDN
                     YLNDGHTLAYYDAFDLRTITKNSSNVVTQWKDKLQNLNRTLTGTAHWHPRDGMIFNIY
                     AANEYLRTETNAWTTGQPFYVYMLVKIDEWNSNGYLIDGRTSPFSPCMLIMLTNNYGF
                     LKIGGSSPNNIFKNIHNITSWGIIRYYLNGENSFFKPDHQIFSGANCGYGVDGITLGQ
                     RYDLDYNYYAKYRLKGLILRDSDANETEIYNCLLKSFQRDNFGTGKVVFTWDGNLKGI
                     KDGIDILDSYNKKSTVWVLADNTYGGVSGNFTFSQVSQWLANGHDIQSHSYNHTRVTN
                     QSEAEFKAMLEQCNAIHIANGWGAIKHFAYPYGSNRPEYHNWASEYILSARGIRENMI
                     WSRTYVDPYDLPAIETDGVKTVNEVKAILDLVKQNRKAVILYAHNVKNEEGYLTPSML
                     AEYIEYAESIGLEIITYDQLYNLLKLTD"
     gene            complement(2049..>3002)
                     /locus_tag="P5293_08025"
     CDS             complement(2049..>3002)
                     /locus_tag="P5293_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49880.1"
                     /translation="LSAGVARLGRPAEGAFTSYGISKFSTLYWDSQYYYYQDEDDNIL
                     REGYRGNYYVIDRTLTELGFDGDEDTDWENIKCSKIYPQIPTNVTLTLISRGVKIDWT
                     AEADDIVEIWGQSEGADYTLLYTIDAGIYTKDDIIPAVSMRYYKLRSYNNLIYSEFTE
                     PVSIAMLGSNIITNTGTPDTTDWIDSNSDGLADEFYGKAGSWSTPACEIITGGGFSGN
                     AQRMNPAALGARVFHSLLGSGLVAGHSYRFRFKLKSNNNFRLYTNNTAELIISQNSTN
                     EVLTTITCYYTVTTGTNLCFYGGSAGYFEIDEFEIKEVLMP"
BASE COUNT          961 a          621 c          350 g         1070 t
ORIGIN      
        1 tttgaattct tttaacagtt atcttaacgc tatctattat ttttctgtgt tgtatggaat
       61 ctgatattga ttttttttta aatcgtcacg cattttttcc atgtcatcag tcaacgaccg
      121 atattgttgt gtattcaatt ttgcataatc tgacagataa tccttgtaac tcctgataaa
      181 gttatcaagc aaaatttgct gattatattg gattgtttta atttcatcaa ctgaattttt
      241 caaagtatct atcttacctc ctatgtcttg ccggaactca acaaactcat cacgtgtctg
      301 tttctgcatc gtaatagtct gacgtgtcga agatacaaac tcaaaaacac cccacactac
      361 accaagtatc gttattaaag ctacaatgct ttttaaccat ctctctacct ttgaaaactc
      421 aaattgtatc atttcttttt attaatctgt taatttcaaa agattatata actggtcgta
      481 agtaataatt tcaagcccaa tactttcggc atactctata tattctgcta acattgaagg
      541 tgttaaatat ccttcttcat tttttacatt atgagcatat aagataactg ctttcctatt
      601 ttgcttaact aaatctaata ttgccttcac ttcattaaca gtttttactc catcagtttc
      661 tattgcaggc aagtcataag gatcaacata agttctactc catatcatat tttccctaat
      721 gcctcttgca gaaagtatgt attctgatgc ccaattgtga tattcaggcc gatttgatcc
      781 atagggatat gcaaaatgtt ttattgcacc ccatccattt gctatatgaa ttgcattaca
      841 ttgttccaac attgctttaa attctgcttc actttggtta gtaactcttg tatgattata
      901 ggaatgactt tgtatgtcat gtccatttgc caaccattgt gaaacttgag aaaatgtaaa
      961 atttccactc acaccaccat aagtattatc ggcaagaacc caaacggtac tttttttatt
     1021 ataactatct aatatatcaa taccatcttt aataccttta agattaccat cccaagtaaa
     1081 tacaacttta cctgtaccaa aattatctct ttgaaatgat tttaatagac aattatatat
     1141 ttctgtttca ttggcatcac tatcccgtaa tattaatcct ttaagtctat atttagcata
     1201 ataattataa tctaaatcat atctttgtcc aagagttatt ccatcaactc cataaccaca
     1261 atttgctcca ctgaatatct gatgatccgg cttaaaaaaa ctattttctc cattaagata
     1321 atatctaata attccccaag aagtaatatt atgtatattt ttaaaaatat tatttggcga
     1381 actaccacct atttttaaaa aaccataatt attagtaagc ataattaaca tacatggaga
     1441 aaaaggactg gttctaccat caattaaata accattagag ttccattcat caatttttac
     1501 aagcatatat acatagaaag gctggccggt agtccaagca tttgtctctg ttcttaaata
     1561 ttcattcgct gcataaatat taaatatcat accatccctg ggatgccaat gtgctgtacc
     1621 ggtaagtgtt ctgttaagat tttgtaattt atctttccac tgtgtaacta catttgaact
     1681 attctttgtt attgttctca aatcaaaagc atcataatat gcaagagtat gcccatcatt
     1741 taaatagtta tccaaaaacc caacttcttc ccaatctata cccgcaatac cattaaatcc
     1801 caaatatgtt ctttcagcat caactttata ttcattattc cttatccctt ctctgaattt
     1861 atttccaaga atctcgtaat tactttttct tacattaacc caccctatat tttctacacc
     1921 agaaaatcca tttgctgtaa gttctgcgtc caccacaaat ttattatttc tcactccaac
     1981 cctcaaatgc cttccatatt gatcaatgac atactgaaat gacctattcg tatattcata
     2041 atattgtctc atggcattaa aacttcttta atttcaaatt catcaatttc aaaataaccg
     2101 gcagatcctc cataaaaaca taaatttgtt ccagtagtaa ccgtataata acatgttata
     2161 gttgtcaata cttcattcgt actattttga ctaattataa gttcggccgt attatttgta
     2221 tataaacgga aattattatt actctttaat ttgaatctaa acctataact atgaccagct
     2281 actaatccac ttcctaaaag agaatgaaat actcttgcac ctaatgctgc gggattcata
     2341 cgctgagcat ttccactaaa tccgccacct gtaataattt cacaagcagg agttgaccac
     2401 gagccagctt ttccataaaa ttcatcagcc aatccatcac tattagaatc aatccagtca
     2461 gtcgtatcag gtgtgcctgt attagtaatt atattgcttc ccagcattgc gatagatacc
     2521 ggttctgtaa attcagaata aattaaatta ttatacgagc gcaacttata atatctcata
     2581 ctaaccgccg gaataatatc atcctttgta taaataccgg catcaattgt ataaagcaag
     2641 gtataatcgg caccttctga ttgaccccat atttcaacaa tatcatctgc ttcggcagtc
     2701 caatcaattt taacacctct acttattaat gttaatgtga catttgttgg tatttgagga
     2761 tatattttac tacatttaat attttcccag tctgtatcct catcaccatc aaaacctaat
     2821 tctgtcaatg ttctatcaat gacatagtaa ttacctcgat aaccctcacg taatatatta
     2881 tcatcttcat cctgataata ataatattgt gaatcccagt ataatgtact aaatttgcta
     2941 ataccataag acgtaaaagc cccctcagcc ggacggccaa gcctcgcaac acctgctgac
     3001 aa
//
LOCUS       DAOTQL010000168         2995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25436_length_2995_cov_3.046259, whole genome shotgun sequence.
ACCESSION   DAOTQL010000168 DAOTQL010000000
VERSION     DAOTQL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25436_length_2995_cov_3.046259"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="P5293_08030"
     CDS             <1..278
                     /locus_tag="P5293_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD-dependent epimerase/dehydratase"
                     /protein_id="HRR49881.1"
                     /translation="GMIKVMDTDDSFIGPINIGNPDEFSIIDLAEKVIRLTGSKSRIV
                     KRDLPMDDPRQRKPDITLAKILLEWEPKIKFDDGLERTIDYFRNKVF"
     gene            281..1420
                     /locus_tag="P5293_08035"
     CDS             281..1420
                     /locus_tag="P5293_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRR49882.1"
                     /translation="MDINNHDYTILIVDDVDANVLLLRLLISKAGYKTMTALNGRDAL
                     EAVNKQKPDLILLDIMMPVMDGHEVAKRLKETPEYADIPIIFLSALNSTEDVVQGFRF
                     GAADYISKPFNKDELLTRINHQISLIESKRIIQRQTEELRKTIIGRDKLYSVIAHDLR
                     GPIASIRMVMELLISEIKPENIGEDMYELLTMANRLTDDSFSLLDNLLKWTKSQTGRL
                     NTVFQDNVDVMNLINGVVEVQKGVAALKKVKINVIGSTNRIARIDIDMAKTSLRNLIS
                     NAIKFSYENSNVDIVIEEAEDRIYIAVRDYGTGISQDNQEKLFKPDIHFTSYGTDNEE
                     GSGLGLLICNEFVRRNGGTLKFTSTEGEGSTFVFDIPTATSLEKK"
     gene            1430..1783
                     /locus_tag="P5293_08040"
     CDS             1430..1783
                     /locus_tag="P5293_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRR49883.1"
                     /translation="MQDSIDNQKYPIPDLSYLRELTDGDADSMKEIISIFMDEAPKLI
                     KMMKESAEVKDHEKLKFATHKLITQLTYVGINSIVPEVKLINSGSVKIKDLDDRIDRI
                     IRVIENSITYLKGLI"
     gene            1800..>2995
                     /locus_tag="P5293_08045"
     CDS             1800..>2995
                     /locus_tag="P5293_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRR49884.1"
                     /translation="MKKILIIDDDKLVRKVFKMTLMKEGYDVLEAEDGPSGLQLIRSE
                     QPDLTITDYQMPGMNGIEVLEEIRKRKMNMPVIILTAFGDVVLTIKAIQLGAYDFLEK
                     PVSPELLKSTVRNALDSVERSKKLVEVVKAGVSSESILENNILVGKTPGMKEIFKSIG
                     MVSMNRVNVLIQGETGTGKEMIARMIHYSGITRSEPLVVVNCSALTETLLESELFGHV
                     KGAFTDSIRDKKGKFELAGEGTIFLDEISEVSLNTQVKLLRVIQELEFERVGGETPIP
                     MRARIITATNKNLEKLIEEGKFREDLYYRLKVFTIDMPPLRERKEDISDLVIHFLYKL
                     NKRYNRNITKIGDGVIEMLQQHNWPGNVRELENTILQAMIVSKNDVLEKENIVLINRF
                     QGDIYK"
BASE COUNT         1049 a          456 c          670 g          820 t
ORIGIN      
        1 atgggatgat aaaagtaatg gatacagacg attcctttat tgggccaatt aacattggga
       61 accctgacga gttctcaatc attgatttgg cagaaaaagt gataaggcta acaggttcaa
      121 aatccaggat tgttaagaga gatttgccaa tggacgatcc ccgtcaacga aagcccgata
      181 ttactctggc aaagattttg ttagaatggg agccgaagat aaagtttgat gacggtttgg
      241 aaaggacaat tgattatttc aggaataagg ttttttagaa atggatataa acaatcatga
      301 ttacacaata ttaattgtgg atgatgtaga tgcaaacgtt cttctcctcc ggttgttaat
      361 atccaaggct ggttataaaa caatgacagc cttaaatggc agggacgcac tcgaagctgt
      421 gaataagcaa aaacctgatc tcattttgct cgacataatg atgcctgtaa tggatggaca
      481 cgaggtggca aaaagattaa aggagacacc tgaatatgcc gatattccaa taatcttttt
      541 gtctgcttta aactctacag aagatgttgt ccaaggtttc agatttggag cagccgatta
      601 catatccaaa ccatttaaca aagatgagct tctcacgaga attaatcatc agatctcact
      661 tattgagtca aagagaatta ttcaaagaca gacagaagag cttaggaaga caataatagg
      721 aagagataaa ctttattcag tcatagcaca tgacctaaga ggtcctatag cctccataag
      781 gatggtaatg gagttactaa tatcagaaat taagcctgaa aacattggag aagacatgta
      841 tgaacttttg acaatggcca acagacttac tgacgacagt ttttctcttc ttgataatct
      901 tctaaagtgg acaaaaagtc agacgggaag gttgaatact gtttttcagg ataatgtcga
      961 tgttatgaac cttattaacg gtgttgtcga ggttcagaaa ggtgtagctg cactaaaaaa
     1021 ggtcaaaatc aacgttattg gttcgacaaa ccgtattgca agaatagata tcgatatggc
     1081 taaaacttcc ctgagaaatt taataagcaa tgccataaag ttcagttacg agaattccaa
     1141 tgtcgacata gttatcgaag aggccgaaga tagaatatac atagcagtac gggattatgg
     1201 tacaggaata agtcaggaca atcaggaaaa gctctttaag cctgacatac attttacaag
     1261 ttatggaacg gacaatgagg agggatccgg actaggactc ttaatatgta acgaatttgt
     1321 taggcgtaac ggaggaacat tgaagtttac ctcaactgaa ggagagggtt ccacttttgt
     1381 ttttgacata ccaactgcca ccagtttgga aaaaaaataa atattttata tgcaagatag
     1441 tatcgataat cagaaatatc ctatacccga tctctcatat ttgagagaat tgacggatgg
     1501 agatgcggat tctatgaaag agataatttc aatatttatg gatgaggctc caaagttaat
     1561 aaaaatgatg aaagagagtg cggaggtaaa ggatcatgaa aaactcaaat ttgctactca
     1621 caaattgata acccagctaa catacgttgg cataaattcg attgttccag aagtcaagct
     1681 cataaattcg ggaagtgtca agattaaaga cttggatgat agaatagata gaatcatccg
     1741 ggtgattgag aacagtataa cttatttgaa aggacttatt taagaacatt taaatctgta
     1801 tgaaaaaaat attaatcata gacgatgaca aactcgttcg caaagtgttt aaaatgacgc
     1861 taatgaagga gggatacgat gttcttgagg ctgaagatgg tccttctgga ttgcaattga
     1921 tacgttctga acaacctgat ttgactatta cagattatca gatgcccgga atgaacggta
     1981 ttgaggtgct tgaagagatc aggaaaagaa agatgaatat gcccgtgatc attcttactg
     2041 catttggcga tgtagtcttg acgattaagg caattcaact tggggcttac gattttcttg
     2101 agaagccggt ttcgccggaa cttttaaaat cgactgtcag gaatgcttta gattctgtag
     2161 agagaagtaa aaagcttgtt gaggttgtta aagcaggtgt atccagtgaa agcatattgg
     2221 aaaataacat tttggtcgga aaaactcccg ggatgaaaga gattttcaaa agtattggga
     2281 tggtctctat gaacagagtg aatgtactta ttcagggaga gacaggtacg ggaaaggaga
     2341 tgattgcaag aatgatacac tactccggaa taactcgttc cgaacccttg gtggttgtta
     2401 attgcagtgc acttactgaa actttgcttg aaagcgaact gtttggacat gtcaaggggg
     2461 cttttacaga ctcaatcagg gataaaaagg gcaagtttga acttgcagga gaggggacta
     2521 ttttccttga tgaaatatca gaagtatctc taaatacaca ggttaaactt ttaagagtta
     2581 ttcaagaact tgaatttgaa agggtaggag gagagactcc tattccgatg agagcaagga
     2641 taattactgc aactaataag aaccttgaaa agcttattga agaaggaaaa ttcagggaag
     2701 atctctatta caggttgaaa gtatttacca ttgatatgcc gccattgagg gagaggaaag
     2761 aggacataag tgatttggtt attcattttc tatataagct taacaaaaga tataacagaa
     2821 atattaccaa aataggtgac ggagtcatcg aaatgcttca gcaacacaat tggccgggta
     2881 atgtcagaga gcttgaaaac acaattctgc aagccatgat tgtttccaag aatgatgtgc
     2941 ttgagaaaga gaatattgta ctaataaaca gattccaggg ggatatttac aagca
//
LOCUS       DAOTQL010000169         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_25942_length_2951_cov_3.482390, whole genome shotgun sequence.
ACCESSION   DAOTQL010000169 DAOTQL010000000
VERSION     DAOTQL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25942_length_2951_cov_3.482390"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="P5293_08050"
     CDS             <1..299
                     /locus_tag="P5293_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49885.1"
                     /translation="SEYTPLTPFPGSLLMEQTKNDITTTEPALYDMQHIVLPTKITLK
                     EFYKQMAKTYLYSHSPINAFRAKLTLRISFNPFSKTMLSYFKFLYNLRRAYLHH"
     gene            366..806
                     /locus_tag="P5293_08055"
     CDS             366..806
                     /locus_tag="P5293_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRR49886.1"
                     /translation="MNVKFSYEEISSEVKSAIAEIVGIEDEELHDDMSLISDLGIDSL
                     DLVDLLFGLGRKFDVEISIQYWTEKIHSESLKIEEVGVENTLLSISELTGLEFDENDS
                     NLLKELISGDYQTWDVVRTILSFIKIKSLCRCIEIKTQENHNEK"
     gene            796..2094
                     /locus_tag="P5293_08060"
     CDS             796..2094
                     /locus_tag="P5293_08060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRR49887.1"
                     /translation="MKNKRVVVTGVGVVTPIGNNLNDFWRNSLEGKSGCKKISLFDTR
                     NFKCKIAAEVKGELAHLPEYLEYDRNEKLSRATVFAITALAEAMENAKLNAKLIDPYE
                     TSIILGAGISNSNFHPLYDIIAEQVFVGADFDFKRLEECINNRIVPLEELENFTCGYN
                     PGSKIASKLGIRGLCRTISTACASGTQAIGEAYRRISRGDTEIVITGGTDAMIDIEGF
                     TLFSILNVMSCRNDEPEKASRPFDAKRDGFVLGEGAGILILESLESAIRRKADILGEV
                     IGFGCTTDINHVTTPEPNGLACANTMKTAIEDAGISKDEVDYINAHGTSTFYNDHIET
                     LGIKNVFGDRAYSIPVTSNKSMIGHLVSAAGAVEFISCLLSMKHSIIPPTINLENPDP
                     ACDLDYVPNKAREASINVSLSNSFGFGGQNSTLIVKKYAE"
     gene            2106..>2951
                     /locus_tag="P5293_08065"
     CDS             2106..>2951
                     /locus_tag="P5293_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRR49888.1"
                     /translation="MKNRVYITGYASIVPKHQEETYSDVFGNRKIQRFSRRSGTLFME
                     AVKNALAMSEINISEINSYRTAIILGEYMNQKQDIDSIINVLKKSISESGKNVFDDGL
                     YSAYSVKEWNTSSALANIPNIPGFLCALSLQNKGYLSTELLTCAGGLFSITEGFSKIQ
                     DGLADIAIAGGTSSKSDLLEVIKYRAMGYYSDDDYQISEGAGVVILEAESSVMKRGVK
                     PYAEILGSANSFSPETYKTKEIDSKSVEFLLKQTMENADLNEEKPIDYITGSFSTKYV
                     DKEIKI"
BASE COUNT         1052 a          400 c          602 g          897 t
ORIGIN      
        1 acagcgaata tactccgctg acaccttttc ctgggagttt gcttatggag caaactaaaa
       61 atgatataac aaccactgaa ccggcactct atgatatgca gcatattgtt ttgccaacca
      121 aaataacttt gaaagagttc tataagcaga tggcaaaaac ttatctttac agccattctc
      181 cgattaatgc ttttcgagca aaattgactt tgcgaatcag ttttaatccg tttagtaaaa
      241 cgatgttgtc gtactttaaa tttctctaca atttgagaag agcttatttg caccattaaa
      301 caaatattta aaataaaaag ttttaattat taagcaataa ataaaaaatt tttaggggga
      361 tttgtatgaa tgtaaaattc agttatgagg aaattagcag tgaagtaaaa tcggccattg
      421 ctgagattgt cggcatagag gatgaagaat tacatgatga tatgtctttg attagtgatt
      481 tgggaattga ctctcttgac ttagttgatt tattattcgg acttggtaga aagtttgatg
      541 tagaaatttc aatacaatac tggacagaga agatacactc agaatctcta aaaattgaag
      601 aagtaggtgt ggaaaacaca ttattgtcca tatcggagct tactggattg gaatttgatg
      661 aaaatgattc aaatttactt aaggagctta taagtggaga ttatcagact tgggacgtgg
      721 tgagaacaat actgagcttt attaaaatta agagtttatg tcgttgtatt gaaataaaaa
      781 ctcaggagaa ccacaatgaa aaataaaaga gttgtagtta caggagttgg tgtagttaca
      841 cctataggga ataatttaaa tgatttctgg cgtaattctc ttgaaggaaa gtctggatgt
      901 aaaaaaattt ctctttttga tactaggaat tttaaatgta aaattgcagc agaagtaaaa
      961 ggtgaattag cacatttacc tgaatatttg gagtatgata gaaatgaaaa gcttagcagg
     1021 gcaacggtat ttgcaataac agctttagca gaggcaatgg aaaatgcgaa gctcaatgca
     1081 aaacttatag atccttatga gacatctata atccttggag caggtatttc aaattcgaat
     1141 ttccatccat tatatgatat tatagccgaa caagtttttg taggtgcaga ttttgacttt
     1201 aaacgactcg aagagtgtat aaataatcga atagtaccat tagaagagct tgagaatttt
     1261 acatgtggat ataatccggg atctaaaatt gcatcaaaac ttggtataag aggtctttgc
     1321 agaacaattt ccacggcttg tgcgtcagga acacaggcta tcggagaagc ttataggaga
     1381 atttcaagag gagacacaga aattgttata actggtggaa cagatgcaat gatcgatatt
     1441 gaaggcttca cattgttttc aattttaaat gttatgtcct gtagaaatga tgaacctgaa
     1501 aaagctagca gaccttttga tgcaaaaagg gatggatttg ttttgggaga aggtgcgggc
     1561 atattaatat tagaaagtct tgaaagtgct ataagaagaa aggccgatat attgggagag
     1621 gttatagggt ttggatgtac tacggatatt aaccatgtga ctactcctga acccaatggt
     1681 ttagcatgtg caaatacaat gaaaacagca attgaagatg caggaatttc aaaagacgaa
     1741 gttgattaca taaatgctca tggtacttct actttttata atgaccatat tgaaacattg
     1801 ggaattaaga atgtgttcgg agaccgagca tattctatac ctgtaacttc aaacaagtca
     1861 atgataggac atttggtttc tgcagctggt gccgttgaat ttatatcttg tctattgagc
     1921 atgaaacatt caattatacc accaacaata aacttggaaa atcctgaccc tgcatgtgat
     1981 ttggattatg ttcctaataa ggcaagagaa gcttctatta atgtttcgtt atctaattca
     2041 tttgggtttg ggggacagaa ttcaactctt atagtaaaaa aatatgctga gtaaaagagg
     2101 tagctatgaa aaatagagta tatattaccg gttatgctag tattgtacca aaacatcaag
     2161 aagaaacata ctcagatgtt tttgggaata gaaaaatcca acgtttttca agaagaagcg
     2221 gtacactgtt tatggaagcc gtaaaaaatg ctttggcaat gtcggagatt aacataagtg
     2281 aaataaacag ctataggaca gcaataatat taggcgagta tatgaatcaa aaacaggata
     2341 ttgattcaat aatcaatgtt ttgaaaaaaa gcatttcgga atcagggaag aatgtttttg
     2401 atgatggctt atattctgct tattcagtta aagaatggaa tacatcgagt gctttagcaa
     2461 atattccaaa tataccagga tttttatgtg cgttatccct tcagaataaa ggatatctct
     2521 caacagaact attaacttgt gctggaggac ttttttcaat tacagaagga ttcagcaaaa
     2581 ttcaagacgg cttggctgat attgctattg ctgggggcac aagttcaaaa tcggatttac
     2641 ttgaagtgat taaatatagg gcaatgggtt attattctga tgacgattat caaatatccg
     2701 aaggtgcagg tgtagttatt cttgaagcag agagtagtgt tatgaaaaga ggagttaaac
     2761 catatgccga gatattaggt tcagctaatt cattttcacc agagacatac aaaacaaaag
     2821 aaatcgactc aaaaagtgtt gagtttttgc tcaaacaaac tatggaaaat gccgatttaa
     2881 atgaggaaaa gccaatagat tatattacag gatcattttc aacaaagtat gttgataaag
     2941 aaattaaaat a
//
LOCUS       DAOTQL010000170         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_26113_length_2937_cov_4.771339, whole genome shotgun sequence.
ACCESSION   DAOTQL010000170 DAOTQL010000000
VERSION     DAOTQL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26113_length_2937_cov_4.771339"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..906)
                     /locus_tag="P5293_08070"
     CDS             complement(49..906)
                     /locus_tag="P5293_08070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRR49889.1"
                     /translation="MTRVKKGKIRILNSKFRFSIFNDTSHEELFVFRAKGLMFVLSIC
                     LAIIFIIASVTILISYTPLREFIPGYPNAQTRKLLVQNALKIDSLERALKLSEYQITN
                     IQRIVTGQKPEEMELAAKPATSADTTVINSKKVKEDSLLRIEVLKQEQLNPTKRKGIE
                     QIEGLHFFPPVKGIISNGFNAALNHPYLDIATPANSVVCAVLDGTVIQANWNDETGYI
                     IEIQHSNDLVSIYKHNSKLLKKVGDKVTAGTAIALTGSTGSLSTGTHLHFELWHKGTA
                     IDPSKYIKF"
     gene            complement(893..1279)
                     /locus_tag="P5293_08075"
     CDS             complement(893..1279)
                     /locus_tag="P5293_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HRR49890.1"
                     /translation="MPRLFTYTDFAKGSDKVKAYADKHTPVIICENEAERDKLFSVKV
                     KLGISTKHPNTAEHHFEFIQLWNLETLIGEIKLQRSSYGKDPLQIEAVFSFYPRVSQR
                     LTALAYCNKHGLWKSEEIFIKVYDKG"
     gene            1330..1746
                     /gene="ruvX"
                     /locus_tag="P5293_08080"
     CDS             1330..1746
                     /gene="ruvX"
                     /locus_tag="P5293_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRR49891.1"
                     /translation="MERIIGIDYGKKRVGVAVSDPLGIFASPLDTVPSAGIIDFLKSY
                     MEKEKVVLIVVGYPLNMNNKPSEAAAYVDQFLNRLRKQFPSLPVKLEDERFTSQLAFQ
                     AMIDGGVKKMDRRVKGNVDKISAAIILQSYLDRNKL"
     gene            1764..2327
                     /gene="def"
                     /locus_tag="P5293_08085"
     CDS             1764..2327
                     /gene="def"
                     /locus_tag="P5293_08085"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRR49892.1"
                     /translation="MVLPIYLYGSEILRKKAEEVDISNMELKDEIRQLIEDMKETMTK
                     ADGVGLAAPQVGKSIRVIVVDGALLGSEDPKLAGFNRAMINPVILEESQQTVEYNEGC
                     LSIPDIHAAVVRSEMIKVSYYDTDFVEHEEILNGFACRMVQHEISHLDGLLFTDLVAP
                     IRKKMIQSRLNNIKSGKVNTFYKAIVK"
     gene            2337..>2937
                     /locus_tag="P5293_08090"
     CDS             2337..>2937
                     /locus_tag="P5293_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49893.1"
                     /translation="MGAFHAYDIRGIYNQDFDKNIAYKVGYFIPELLHTDKVLVGRDA
                     RISSPEIHQALTSGIMDAGADVFDLGLSTTPMVYYATAKYGFKASAQITASHNPKEYN
                     GLKVSGENALPVGLDSGLGTIKSWIDDGRETPVACKRGAINNFDVKSEYVHFLMQYKK
                     DISNLNIAVDLSNGMAALLIKDILGHEGERLHYIFDELDG"
BASE COUNT          906 a          513 c          595 g          923 t
ORIGIN      
        1 agagcctgtt gatccgagaa ctgcaactct tcgcttcatc atatgagatt agaatttaat
       61 atacttggaa ggatcaatag cggttccttt atgccataac tcgaaatgaa gatgagttcc
      121 ggtactcaaa gatcctgtgc tgcctgtaag agcaatagct gtgcctgcag taactttgtc
      181 tcccaccttc tttagtaatt tagagttatg tttatatata gacaccaaat cattgctgtg
      241 ttgaatttca attatatatc ctgtttcatc attccaattt gcctggataa cggtgccgtc
      301 aaggacagca cacaccaccg aattggcagg ggttgcaatg tcaagatagg gatgattgag
      361 agcagcgtta aacccgttag agatgatccc tttgacaggt ggaaaaaaat gaagcccttc
      421 aatttgttct atcccctttc tttttgtggg gttaagttgc tcctgcttca atacttcaat
      481 ccttagcaag gaatcttctt tgactttctt gctatttata acagtagtat cagcagaggt
      541 ggcaggtttt gctgcaagtt ccatctcttc aggcttttgt cccgtaacga ttctttgaat
      601 atttgtaatt tgatattctg aaagctttaa ggctcgctca agagaatcaa ttttaagagc
      661 attttgcact agcaattttc ttgtctgggc gtttggatat cccggaataa attcacgaag
      721 aggtgtatat gaaataagaa ttgtaacaga agctattata aatataatag caagacagat
      781 actcaataca aacatcaatc ctttagctct gaaaacaaac agctcttcat gtgatgtatc
      841 gttgaatatt gaaaacctga acttgctgtt aagtattcta atttttcctt ttttaaccct
      901 tgtcatatac ttttataaat atctcttcgc ttttccacaa tccgtgctta ttacagtacg
      961 caagtgccgt gagtcgctgg ctgactcttg gatagaagct gaaaacagct tctatctgga
     1021 gaggatcttt accataactg cttctttgga gtttaatctc tccaattaaa gtttcaaggt
     1081 tccagagttg aataaattca aaatggtgtt ctgcagtgtt tggatgtttt gtagaaatac
     1141 ccaattttac cttaactgag aagagtttgt ctctttcagc ctcattttca caaataataa
     1201 caggagtgtg tttgtcggca taagccttca ccttatcact tccttttgca aaatcggtgt
     1261 atgtgaatag tcgtggcata atgattttat gagtaacaaa agtactaaaa aaataactaa
     1321 atttgcgtaa tggagcgtat tataggcata gattacggca aaaagagagt tggagtagca
     1381 gtcagcgatc ctcttggcat ttttgcttct cctcttgaca cagttccttc tgccgggata
     1441 atcgactttt taaaaagtta catggaaaaa gaaaaagttg tgttgatagt tgtcggatat
     1501 cctctcaaca tgaacaataa accttctgaa gctgcagcgt atgttgatca atttcttaac
     1561 aggcttagga aacaatttcc ctcgctacct gttaaattgg aagatgaacg ttttacatct
     1621 caacttgctt ttcaagctat gattgatggc ggagtaaaaa aaatggatcg cagagttaag
     1681 ggaaatgttg acaagataag tgctgccata attttacaaa gttaccttga caggaacaaa
     1741 ttataatttt aaataacaac ataatggtac ttccgattta tttatatgga tctgaaattc
     1801 ttcgaaaaaa agcagaagag gttgatattt ccaatatgga acttaaggat gaaatacgac
     1861 agcttataga agatatgaaa gagacgatga ccaaagcaga tggcgtaggt cttgctgcgc
     1921 cccaagttgg taaatcgatc agagttatag ttgttgatgg agctttactt ggaagtgaag
     1981 atccgaaact tgccggattt aatagagcaa tgatcaatcc cgtcattttg gaagagagtc
     2041 agcagacagt cgaatacaat gaaggatgcc tcagtattcc agatatacat gcagctgtcg
     2101 tgagatctga aatgatcaaa gtttcatatt atgatactga ctttgtcgag catgaagaga
     2161 tccttaatgg atttgcctgc cggatggttc agcacgaaat tagccatctt gatggtttgc
     2221 tctttacaga tcttgtagct cctatcagga aaaagatgat acagagtagg ctcaataata
     2281 taaaatctgg aaaggttaac actttctaca aagcaatagt caaataattt agatttatgg
     2341 gagcttttca tgcttacgat attagaggaa tttataatca ggattttgac aaaaatattg
     2401 cctataaggt tggatatttt attcctgaac ttttgcacac agacaaagtc cttgtaggca
     2461 gggatgcaag aatatcatct cctgaaatac accaggcgct gacatcgggc ataatggatg
     2521 caggagctga tgtgtttgat ctcggtcttt caactacgcc gatggtgtat tatgcaactg
     2581 ccaaatatgg ttttaaagct tctgctcaga taactgcttc tcataatcct aaggagtata
     2641 atggtcttaa agtttcagga gagaatgcac ttcctgtagg cttagattca ggacttggca
     2701 caattaaaag ttggatagac gatggacgag aaacccctgt ggcttgtaaa aggggagcaa
     2761 ttaacaattt cgacgttaaa agtgagtatg tgcatttttt aatgcaatac aaaaaagata
     2821 ttagcaatct taatattgct gtagaccttt caaatggcat ggcagctctt ttgatcaaag
     2881 atattcttgg acatgaagga gagcgattgc actacatttt tgacgaactt gacggca
//
LOCUS       DAOTQL010000171         2931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_26179_length_2931_cov_4.196801, whole genome shotgun sequence.
ACCESSION   DAOTQL010000171 DAOTQL010000000
VERSION     DAOTQL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26179_length_2931_cov_4.196801"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2277)
                     /locus_tag="P5293_08095"
     CDS             complement(<1..2277)
                     /locus_tag="P5293_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRR49894.1"
                     /translation="MSDVIEEAVDFVGQESNKELIKWVNSSENGSLVVEGLCCSAKSL
                     AMASAMKRGIHMVILNSREDAVYFASDLSALLDSESVYFFPSSVNTALRNKHYDSSSQ
                     VQRTAAIKALSSYSDNSTNNNGRVILVGYPESFSEKVVNKKKLKSNILKISTGDALSH
                     EFVKETLQLNSFRRVDFVQEPGEYALRGSIIDLFSFSDSKPYRLDFFGDQIESIKVFD
                     IDTQRSLEEIQSVEIYPNIYEGTESEELIDVFSFTGNNSILWLCDYDFFAQKLEFLED
                     LTKHKKIFLSSVSSNILCDKKIFYKTVPQPVFNKNFELLAREIKERQNAGYKVYILSD
                     NSHQIDRLRSIFSSSFSDDKVIFLTKSISLHEGFIDHIAKLCIFSDHQIFERYHRVKP
                     NRSVERSERLTINELNSYQIGDYIVHIDHGIGIFGGLVKTNINGKQQEAVKIMYKDGD
                     VIFVSIHGLHRISKYKSADSQPPKIYKLGTGAWQKLKSQTKSKVKDIAKDLILLYAKR
                     MDSKGFAFSPDSYLQNELEASFQFEDTPDQIKATIAVKEDMEQPYPMDRLICGDVGFG
                     KTEVAIRAAFKAVIDGKQVALLVPTTILALQHYKTFTSRLKDFPCTVDYICRLRGSKE
                     ISEISKQIENGKVDIIIGTHRLLNKEIKFKDLGLLIIDEEQKFGVAAKERLRQMKTNV
                     DTLTMTATPIPRTLQFSLLGSRDLSIINTPPPNRLPVETEIISFDEDIIRDAINYEVE
                     RGGQVFFVHNRVENIKSVE"
     gene            2693..>2931
                     /locus_tag="P5293_08100"
     CDS             2693..>2931
                     /locus_tag="P5293_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRR49895.1"
                     /translation="MSMKAFDPREERENKDKDFDGEIRPQDFSEFAGQEKIIENLKIF
                     VKAAMLRGEALDHILLHGPPGLGKTTLAHIVANELG"
BASE COUNT          980 a          508 c          434 g         1009 t
ORIGIN      
        1 ttcaacactt ttaatatttt ccactctgtt atgcacaaaa aaaacctgac ctcctcgttc
       61 tacttcataa ttaatagcat ctctaataat atcctcgtca aaactaataa tttcagtctc
      121 aaccggaagt ctgttgggtg gcggagtatt aataattgaa agatccctgg atcccaaaag
      181 agaaaattga agagtcctcg gtattggagt tgcagtcata gtcaatgtgt ccacattcgt
      241 tttcatttgt ctgagtctct ctttggcagc aacaccaaat ttctgctctt catcaataat
      301 tagcagccca agatccttaa acttaatttc cttatttagc agtctgtgcg taccaattat
      361 aatatcaact ttcccatttt caatctgttt tgaaatttca gatatctctt ttgaacctct
      421 tagtctgcat atgtaatcaa ccgtacatgg aaagtcttta agacgtgaag taaaagtttt
      481 atagtgctga agagccagta ttgttgtagg gactagtaaa gcaacctgct taccgtcgat
      541 aactgcttta aatgcagccc ttattgccac ttcagtcttc ccaaaaccca catctccaca
      601 tataagacga tccataggat aaggctgttc catatcttcc tttactgcta ttgttgcttt
      661 tatctgatca ggtgtatcct caaactgaaa tgatgcctca agttcattct gcaaataaga
      721 atcaggagaa aaagcaaatc cttttgaatc cattcttttt gcatatagca aaatcaagtc
      781 tttggcaata tccttaacct ttgactttgt ctgactcttg agtttttgcc aggcaccagt
      841 gccaagttta tatattttag gtggttggga atcagcagat ttgtactttg agatacggtg
      901 gaggccatga attgagacaa aaattacatc tccgtccttg tacattattt ttacagcttc
      961 ttgctgtttt ccgttgatat ttgtctttac aagacctccg aaaataccta tgccgtgatc
     1021 aatgtgaaca atataatctc ctatctgata tgaattgagt tcattaatag ttagtctttc
     1081 acttctctca acacttctgt taggctttac cctgtgatat ctttcaaaaa tctgatgatc
     1141 tgaaaagata catagtttag ctatatgatc aataaatcct tcatgaagag atatggattt
     1201 agtcagaaaa atcaccttat catctgaaaa ggaagaagaa aaaatgctcc tgagtctgtc
     1261 aatttgatga gagttatcac ttaatatata aactttgtaa ccagcgttct gcctttcttt
     1321 gatttctctt gcaagcagtt caaagttttt attaaatacc ggttgtggaa cagttttata
     1381 aaagattttt ttatcacata aaatatttga agatacagat gataaaaaaa tttttttatg
     1441 ctttgtcaga tcttctaaaa attccaactt ttgagcaaaa aaatcataat cacacaacca
     1501 taaaatagaa ttgtttcctg tgaaagaaaa aacatctatt aattcttctg attctgtacc
     1561 ctcgtatata ttaggataaa tctctacgct ttgtatctcc tctaaagacc tttgagtgtc
     1621 aatatcgaaa acttttatgc tttcaatctg gtcgccgaaa aaatccagtc tgtatggctt
     1681 actgtctgaa aatgaaaata gatctataat actacccctt agtgcatatt ctccaggttc
     1741 ttgcacaaaa tcaacccttc taaaagaatt gagttggagc gtttctttta caaactcatg
     1801 tgaaagggcg tccccagtag aaatttttaa aatatttgat ttaagttttt tcttattaac
     1861 tactttttct gagaaggatt caggataccc aacaagaata actcgcccgt tattattagt
     1921 actattatct gaataagaac tcaatgcttt gatagccgca gttctttgaa cctgagacga
     1981 agagtcgtaa tgtttatttc taagagctgt attaactgaa gatgggaaaa aataaacact
     2041 ttcactatct aaaagagcag aaagatctga agcaaaataa accgcatctt ctctgctgtt
     2101 taaaataacc atatgaatcc ctcttttcat ggcagatgcc atggctaaag atttggccga
     2161 acagcataat ccttctacaa caagagatcc gttttctgaa ctatttaccc atttaataag
     2221 ctctttatta gactcttgtc ctacgaaatc taccgcttct tctataacat cactcataca
     2281 ctgcagcaaa tatagtttat taacattaaa aataaagagc tgtttatatt aatttgattg
     2341 tgtgttgaaa attattgata agttattttt tctatttcag aaaaatttat atactaaaaa
     2401 gtatctgaat tttccaaatt tttcaataat taattatcat caatctatcc aataattttt
     2461 aacaataata attgttgaaa aaaattatgt tatgatatta actaatgtta ataagaatcc
     2521 taaattatta tttattaata gattaatttt aaatatagtg ttattaaaat tgtttatata
     2581 tgttaataag tagttttttt agttaataag aaacattata aacatattaa cagacaataa
     2641 ataatatcaa actatattat aaaaagaata atatatattt gtataaagat aaatttcaat
     2701 gaaagctttt gatcctcgtg aagagagaga aaacaaagat aaagattttg atggagagat
     2761 cagaccacag gatttttctg aatttgcagg ccaagaaaaa attatcgaaa atctcaaaat
     2821 ttttgtaaaa gccgctatgc ttagaggaga agctttagat catattttgc ttcatggacc
     2881 tccgggattg ggaaaaacaa cacttgcaca tatcgtggct aatgagctgg g
//
LOCUS       DAOTQL010000172         2927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_26223_length_2927_cov_4.969011, whole genome shotgun sequence.
ACCESSION   DAOTQL010000172 DAOTQL010000000
VERSION     DAOTQL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26223_length_2927_cov_4.969011"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="P5293_08105"
     CDS             complement(<1..255)
                     /locus_tag="P5293_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRR49896.1"
                     /translation="MSEKLISRVPEGPLSQKWTEHKAHIKVVSPANKRKLEIIVIGTG
                     LGGASAAASLGELGYNVKVFCISDSPRRAHSIAAQGGINAA"
     gene            393..2588
                     /locus_tag="P5293_08110"
     CDS             393..2588
                     /locus_tag="P5293_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRR49897.1"
                     /translation="MKKTLSFLITLCISFSLSAQSDIIPKPQKEIYKDGFFVIDKNCV
                     IYSNEKNAFNCNYLQDKIQKATGFPFTKVKKHPLNNYVILEVDPQMEIPVEGYVLKSD
                     STGIIIKAKSKAGIFYGVQSFLQLLPPAVYSGRTTGREEWKIPICNIEDYPRFSYRGM
                     MLDVSRTFFDYDVIIKYIDWLSYHKINKFHWHLADDNGWRIEIKKYPELTTKGAWRGP
                     EEVLQPSYGSGNKRYGGFYTQEQIKKIVAYAAERNIEIIPEIDLPGHSRAVTSTYPQV
                     LCRKTDTSLSIQGEGKNVWCVSKESNYKMLEDIIAEIAHLFPSKYIHIGGDEVNYSAW
                     KNCPDCNALMEKMGMKEPAELLGYFVKRMENIVEKHDKHMAGWDEIIDGEKLRPETRV
                     YAWKSVDRGINAVMKGQPTVMQPSQFCYLDMKQSEVERGHNWAGIVTLEKTYSFDPAA
                     KNQIPDSLNYMVLGVQGGLWTELLNKPARFIEYQTFPRLASLAEVGWTNQELRDWNDF
                     KDRIEKKHYNRMFNMGISFRLPPPVITYEGEALKSQLPYPWAVVRYTSDESNPTEFSP
                     VFRGEIYTDNPFKYRFATFYKDELISQTVKVSNVSYNYQKPATKIETSILFQSKFPLE
                     NICDYNFNTYSRSIGKLKEGDYLLYLFDNPVCTKKITVTSGIPQIDFYTITDGYVEYS
                     YDGKEFIKGNKFTEGISVIYPEKPVLAVKIVITGANDGSTAAFNDLQID"
     gene            complement(2585..>2927)
                     /locus_tag="P5293_08115"
     CDS             complement(2585..>2927)
                     /locus_tag="P5293_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49898.1"
                     /translation="AFPLWVKAGDNQPSVMVKSTTSNNAFACDQALRLKNLTFSLKRG
                     NGSKYMNFNLIYNSKNGGYMSRTINADSSIFLLFERNIEKWRVNGLNLHELFELNKQA
                     ENIVIKTYSSI"
BASE COUNT         1052 a          477 c          533 g          865 t
ORIGIN      
        1 tgcagcatta atacctccct gagctgctat agaatgtgct cttcgaggag agtcactgat
       61 acagaaaact ttaacattat atccaagttc tccaagtgat gctgcagctg aagcccctcc
      121 taaacctgta ccaataacaa taatttctaa ttttcttttg ttagcaggac ttacaacttt
      181 tatatgtgcc ttatgttctg tccacttctg ggacaatggg ccttcaggaa ctcttgaaat
      241 cagtttttcg gacattttaa actattttaa aaagttcaca aaggtaggca tattaagata
      301 gatagcaaat tctaaatcaa tttttgtaac tttacaacgc cagaatttcg ggaatataaa
      361 atcatgtata acagataatt atcaatagaa ttatgaaaaa aacattatct tttcttataa
      421 ctttgtgtat ctccttctcc ttaagtgctc aaagtgatat aataccaaaa cctcaaaaag
      481 agatctataa ggatggattc tttgttattg acaaaaattg tgtaatctac agcaacgaaa
      541 aaaatgcttt taattgtaac tatcttcagg ataaaattca aaaagccaca ggattcccct
      601 ttaccaaagt caaaaaacat cctttaaaca attatgttat acttgaagtt gacccacaga
      661 tggaaatccc tgtagaagga tatgttttga aaagcgattc aaccggaatt ataattaaag
      721 caaagtcaaa ggccggaatt ttttatggtg tacagtcatt tctccaatta cttccaccgg
      781 cggtatattc aggaaggaca accgggagag aggagtggaa gattccaatt tgtaatatag
      841 aagattatcc aagattttca tacagaggaa tgatgctcga cgtttcacgg acattttttg
      901 attatgatgt gataatcaaa tatatagact ggttatctta ccataaaatt aacaagtttc
      961 attggcatct ggctgacgat aacggatgga gaatagaaat aaaaaaatat cctgaactta
     1021 ctaccaaagg agcatggaga ggcccggaag aagtattgca accatcgtat ggctcaggta
     1081 acaaaagata cggaggtttt tatactcagg aacagatcaa aaaaatagtt gcttatgcag
     1141 cagaaaggaa tattgaaata attcctgaaa ttgaccttcc aggccacagt agagctgtaa
     1201 catcaactta tcctcaggtt ttatgtagaa aaactgatac aagcctcagt attcagggag
     1261 aagggaaaaa tgtctggtgt gttagtaaag aaagtaatta caaaatgctt gaagatatta
     1321 tagcagaaat tgcacatctt tttccttcaa aatatattca tataggagga gatgaggtaa
     1381 actactccgc ctggaaaaat tgtccggatt gcaatgcttt aatggaaaag atgggaatga
     1441 aggaaccggc tgaattatta ggttactttg ttaaaagaat ggaaaatatt gtcgaaaaac
     1501 atgataagca tatggcagga tgggatgaaa tcattgatgg cgaaaaactt agaccggaaa
     1561 ccagagttta tgcatggaaa agtgttgaca gaggaattaa cgcagttatg aagggacagc
     1621 caacagtaat gcagcccagc caattttgtt acttggatat gaagcagtct gaagtagaaa
     1681 gaggacacaa ctgggcagga attgtaactc ttgaaaaaac ctattcattt gatcctgctg
     1741 caaagaatca aatacctgac agtttaaatt atatggtact cggggttcaa ggaggtttat
     1801 ggacagaact tctcaataaa cctgcaagat ttattgaata tcagacattt cccagacttg
     1861 catctctggc agaagtaggt tggacaaatc aggaacttag agactggaat gattttaaag
     1921 ataggattga aaagaaacat tacaacagaa tgttcaatat gggaatatct ttccggcttc
     1981 ctccaccagt aattacctat gaaggtgaag cattgaaaag ccaacttcca tatccttggg
     2041 cagtagtaag atatacttct gatgaaagta acccaacaga attttctcct gtcttcagag
     2101 gagaaatcta tacagacaac ccttttaaat atagatttgc aacgttttat aaagatgaat
     2161 taataagcca gacagtaaag gttagtaatg tttcatataa ttaccaaaag cctgcaacca
     2221 aaattgaaac atcaatatta ttccagtcaa aattcccttt ggaaaacatt tgtgactata
     2281 attttaacac ttattcaaga agtattggaa aattaaaaga aggagattat ctcttgtatc
     2341 tttttgacaa tcctgtatgc acaaaaaaga ttactgttac ttcaggaatc ccacaaatag
     2401 acttttatac aattactgat ggctacgttg aatactccta tgacggaaaa gagtttatca
     2461 aaggaaataa atttacagag ggaatttctg taatttaccc tgaaaaacca gtattggcag
     2521 ttaaaattgt aataactgga gcaaatgatg gttcaacagc tgcatttaat gacctgcaaa
     2581 tagattaaat acttgaatat gttttaatta ctatattctc agcctgtttg tttaactcaa
     2641 agagttcatg tagattaaga ccgttaactc tccatttctc aatatttctt tcaaaaagta
     2701 aaaaaataga ggagtctgca ttaatggtgc gactcatgta accaccattt tttgaattat
     2761 atattaaatt gaaattcatg tatttacttc cgttaccgcg ttttaatgag aatgttaaat
     2821 ttttaagacg aagagcctgg tcgcaagcaa aagcattatt tgaagttgta gatttaacca
     2881 ttacagaagg ttgattatct ccagccttta cccatagagg aaaagcg
//
LOCUS       DAOTQL010000173         2854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27110_length_2854_cov_4.972847, whole genome shotgun sequence.
ACCESSION   DAOTQL010000173 DAOTQL010000000
VERSION     DAOTQL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27110_length_2854_cov_4.972847"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="P5293_08120"
     CDS             complement(<1..751)
                     /locus_tag="P5293_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRR49899.1"
                     /translation="MICLSIKEKGLNSVQDALSKVHLAEIRLDYTNLTREETVTLFRT
                     KKDLIATCRLTELPVEECRKRLTWAIMASRTKKTVGKRYIDIEYDSPSDYRNDLITAA
                     HKRGFNIILSYHNYKNTDSFERLCEIYEACKSSGADIIKIVTTAQSMHDCTRVLRLYK
                     EYKGIPLIAFCMGETARFTRILSLYLGAPFTFVSLSHTKESAEGQFTIAEAGSLLNKK
                     SYPNKVNGKILNELLVAPASKSHSQRAILAAA"
     gene            complement(738..1742)
                     /locus_tag="P5293_08125"
     CDS             complement(738..1742)
                     /locus_tag="P5293_08125"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HRR49900.1"
                     /translation="MERCVLNLNYCADIDCIDSFIPRDCLVVTIIDAKIAEIYGKYFP
                     TPQIRVVASENNKSLQSVESISLQLLEMGVDRSSFLLAVGGGVISDLVGFIGSIYMRG
                     IKFAYVPTTLLAQVDAAVGGKTAVNLSGFKNILGVVNQPAFTLICPEFLNSLPKNEML
                     AGLSEMLKTFIIADRDNYFNFLGEVRSRQLEAKILWPFIKKCSSIKASIVEQDPFDNG
                     VRKLLNLGHTFAHALEKITQLHHGEAVSIGIILASKLSVKLSLLQSDEELTIESDFKD
                     LGLPVASPVKVSSLTDVMLKDKKKTGGNLDLILIESIGKAIIYSINVKDLEGLLNDLS
                     "
     gene            1862..>2854
                     /locus_tag="P5293_08130"
     CDS             1862..>2854
                     /locus_tag="P5293_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRR49901.1"
                     /translation="MLCRTFCSFCLGIEAIIVEVEVEITQGISFFLVGLPDNAVRESQ
                     QRIEAALGSIGCHLPGKKVIINLAPGNIRKEGTGFDLAIAIGILAASGQYAFKSLSDC
                     VIVGELSLDGTLRPVPGALPIAISCKHEGFKYCIFPEESAYEASAVDGITIYGAKNLL
                     ETVTLLCGESFKEPVTYNDKSEENICSTKVNIPDFSDIKGQESAKRGLEIAASGGHNI
                     LLCGAPGSGKSLMAKALAGILPPMNRNESLETSIVYSVAGSGNSFNGLIKERPFRSPH
                     HSTSLYAMTGGGASAQPGEVSLAHNGVLYLDELPEFSRSVLEVLRQPMEERVISI"
BASE COUNT          920 a          589 c          528 g          817 t
ORIGIN      
        1 aggcagctgc aagtattgct ctttgagaat gactcttgga agccggagca accaacaatt
       61 cattcaaaat cttcccgttt accttattcg gatagctttt tttgttcaac aagctcccgg
      121 cttcagcaat agtaaattga ccttctgcac tctcctttgt atgcgaaagc gagacaaaag
      181 taaacggagc acctaaataa agagataaaa tcctagtaaa tctggcagtc tcacccatac
      241 aaaaagcaat aagaggaatc cccttatatt ccttgtaaag ccttagaacc cttgtacaat
      301 catgcatcga ttgagcagtt gtgacaattt taataatatc ggcacctgaa cttttacaag
      361 cctcatatat ttcacaaagc ctctcaaaac tgtcagtgtt tttataatta tgatatgaga
      421 gaataatatt gaatcccctc ttgtgagcag cagttataag atcatttctg tagtctgaag
      481 gtgaatcata ttcaatgtct atatatcttt tgccaaccgt tttttttgtg cggctggcca
      541 taatggccca tgtcagtctc tttctgcact cctcgacagg aagctcggta agacggcatg
      601 tggcaatcag atcctttttt gtacgaaaaa gagtaaccgt ctcttctctc gtaaggttag
      661 tgtaatcaag ccttatttct gctaaatgca cctttgagag cgcatcctgt acgctgttga
      721 gacctttttc ctttatgcta agacaaatca tttaacaaac cctccaaatc cttaacatta
      781 atagaatata taattgcttt gccaatactt tcaataagaa ttaaatcaag attacctccc
      841 gtcttcttct tatctttcag cataacatca gtcaaagaag acacctttac aggagaagcg
      901 acaggcaaac ccaaatcttt gaaatcagat tcaatagtaa gttcttcatc cgattgcaat
      961 agagacagtt taacagaaag tttggacgca agaattattc cgattgagac agcctctcca
     1021 tgatgtaatt gtgttatctt ttcaagtgca tgagcaaaag tatgtccaag attaagcagt
     1081 ttacgtaccc cattgtcaaa cggatcttgt tcgacaatag aagctttgat cgagctgcat
     1141 tttttaataa aaggccaaag aatttttgct tcaagctgtc ggctacgaac ctctccaaga
     1201 aaattaaagt aattatctct gtcagcaatt ataaaagttt tcaacatctc agaaagcccc
     1261 gctaacatct catttttagg caacgaatta agaaattcag gacaaataag cgtgaaagcg
     1321 ggctgattga caacacctaa aatattcttg aagccggata aatttacggc agtttttcct
     1381 ccaacagcag catcaacttg tgcgagcaaa gttgtaggaa cataagcaaa cttaatacca
     1441 cgcatataga ttgagcctat gaaacccaca agatcagaaa taactccacc tccaaccgcg
     1501 agtaaaaaag agcttctgtc aacccccatt tcaagaagtt gaagagatat actctccaca
     1561 ctctgcaagc ttttattatt ctctgaagca acaactctta tctgaggcgt aggaaaatat
     1621 tttccataga tctctgcgat cttcgcgtct atgattgtaa ccaccaggca atcccggggg
     1681 ataaaagaat caatacaatc tatgtcggca caatagttta ggttaagtac acaccgttcc
     1741 ataattacaa aattaacaaa ttattgaaaa gacagcatca gatatttttt attttgaaac
     1801 caataatcta acgttcagat atgtacaatt ttacaatatt cattctactt ttaaagaaaa
     1861 aatgctttgt cgtacttttt gtagtttttg ccttggcata gaggctatta tagtagaggt
     1921 agaagttgag ataactcaag gcatcagctt tttccttgtg ggactacctg acaatgccgt
     1981 aagagagagt cagcaaagaa ttgaggccgc acttggaagc ataggttgcc atcttcccgg
     2041 taaaaaagtt ataattaacc ttgcaccagg aaatattaga aaagaaggaa ctggttttga
     2101 tctggcaatt gctattggaa tattagctgc ttcgggtcaa tatgcgttta aatcactatc
     2161 agactgcgta atagtcggag aactttcact tgacggaaca ctaagaccgg tcccgggcgc
     2221 tttgcctata gcaatttctt gcaaacacga aggatttaaa tattgcatat tccccgaaga
     2281 atctgcctat gaagcatcgg cagtagacgg aattacaata tacggagcaa aaaatttgct
     2341 tgaaactgta acacttcttt gtggagagtc ttttaaagag cctgttactt ataacgataa
     2401 atcagaagaa aatatatgta gtacaaaagt caatattccc gatttttcag atataaaggg
     2461 tcaagagagt gctaaaagag gacttgaaat agcagcaagt ggaggacata acatactcct
     2521 ttgtggagca cccggttcgg gcaaaagtct aatggccaaa gcacttgccg gcatacttcc
     2581 accaatgaac agaaatgaat cattggaaac aagtatagta tactcagtgg cggggagcgg
     2641 aaattcattt aacggattaa ttaaagaacg tcccttccgc tccccgcatc actccacatc
     2701 attatatgcc atgacaggcg gaggggcttc tgcccaaccc ggagaggtgt cgctcgctca
     2761 caatggagta ctttatctgg atgagttacc ggaattttca cgttctgtcc ttgaagttct
     2821 tcgccagcca atggaagaaa gagttatatc aatt
//
LOCUS       DAOTQL010000174         2854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27114_length_2854_cov_3.952483, whole genome shotgun sequence.
ACCESSION   DAOTQL010000174 DAOTQL010000000
VERSION     DAOTQL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27114_length_2854_cov_3.952483"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="P5293_08135"
     CDS             complement(<1..462)
                     /locus_tag="P5293_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49902.1"
                     /translation="MRVDIHVNPEFSSLNRFIELLPDNFDNTGEVIHLGRNQIRTVEI
                     NGEKLVIKYFKKITLFNRFIYGNLRKSKAKRSFENSVTLLKNGIGTPKPVGYIDTFSH
                     LLLKKSFYICLYTDYTDIKNLLDLQLSEAENGIRALARVSGKMHHSGIFHGD"
     gene            complement(459..1271)
                     /locus_tag="P5293_08140"
     CDS             complement(459..1271)
                     /locus_tag="P5293_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR49903.1"
                     /translation="MEAFFVIDNISCGYGNKFKISEISISVQQGNFLGIIGPNGSGKT
                     TLFRAITGDLKFESGSIKLNGKNLKKFSYKERAQNIAIVTQDIESPDITVEDYVLMGR
                     LPFHKGYQFFESKEDVRIAEKYMKMTAVNKMKNKYLSQLSGGERQMVAITRALVQEPK
                     LLLLDEPTSHLDIALQIQILNLVQQLNAELGLTVLMIIHDLNLAGEYCDELIMMSNGK
                     IKDIGSPEKVLTYSNIEDVYGTVVFTQPNPLSGRPSIFLVSKRILNSINNTK"
     gene            complement(1282..>1625)
                     /locus_tag="P5293_08145"
     CDS             complement(1282..>1625)
                     /locus_tag="P5293_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRR49904.1"
                     /translation="DKARHLGINTDLSIKLLFIISSLLTGMCVSIAGVIGFVGLIIPQ
                     FMRLIVGSDFRILLISSFLTGGGFIVFCDTIARTIVAPNELPVGVITGIIGGISFIAI
                     LINLGKNTRHI"
     assembly_gap    1626..1725
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1726..2456)
                     /locus_tag="P5293_08150"
     CDS             complement(<1726..2456)
                     /locus_tag="P5293_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRR49905.1"
                     /translation="MSRSKYIKWGLWLITFLLIAVMTVIFSLAKGEIDIPLSKIYNIL
                     INRNNDLEYTVLKEIRLPRIILGLSVGGALSLAGVILQGIYRNPLVEPYTLGISGGAA
                     LGVAFAIVAGLNFSVGSFMLPASGFVGALITVFLVYILSVRKGKIHTQNMLLIGVMVS
                     FIASSAIMFIMATTTNENLHNIVFWVMGSLDETNKDLIKVAVIVSMLCLLISYIFIDP
                     LNALRLGEDKARHLGINTDLSIKLL"
     gene            complement(2453..>2854)
                     /locus_tag="P5293_08155"
     CDS             complement(2453..>2854)
                     /locus_tag="P5293_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRR49906.1"
                     /translation="EYQKRRLDSIKKSIPKGEKPKIFIELGAKPLFTAVPNTFMDDFI
                     AQAGGINIATDISTGIITRENVILKDPDAIVIVTMGIVGEEEKAVWKRYKQLSAARKG
                     KIFTVDPYKSCGPNPVTFVDVVEELVTKLYK"
BASE COUNT          863 a          520 c          441 g          930 t
ORIGIN      
        1 atctccatga aagatacctg aatgatgcat ctttcctgac actcttgcca aagctcttat
       61 tccgttctca gcctcagaca gttgtaaatc aagtaaattt tttatatctg tgtaatctgt
      121 atacaggcag atataaaaac ttttcttaag aagaagatga ctgaacgtat caatatatcc
      181 aacaggttta ggggttccta ttccattttt caatagagtt actgaatttt caaaagacct
      241 tttagctttg ctttttctca gatttccata tataaatctg ttgaaaagag taattttttt
      301 gaaatatttt ataacaagtt tttcgccgtt tatttcaaca gttcttattt gatttcttcc
      361 aagatgaata acttctccag tattatcaaa attgtctggc agcagttcaa taaatcggtt
      421 caatgaacta aattcggggt taacatgaat atctactctc attttgtgtt gtttatacta
      481 ttgagtatcc tttttgagac taaaaaaatt gaaggtcttc ctgataaagg attgggctga
      541 gtaaatacta ctgtaccata tacgtcttcg atgtttgaat atgtaagtac tttttctggg
      601 gaacctatat cctttatttt accattactc atcatgatta attcgtcaca atattctcct
      661 gccagattaa gatcgtgaat aatcatcaat actgtcagac ccaattctgc attaagttgt
      721 tgtaccagat taagaatctg gatttgtaga gcaatatcga gatgagaggt tggttcatca
      781 agtaaaagaa gtttgggctc ctgtacaaga gctcttgtaa ttgcaaccat ttgtctctct
      841 ccaccactta attgagagag atatttattt ttcattttat taacagccgt cattttcata
      901 tacttttctg ctattctgac atcctctttg ctttcaaaaa actgataccc cttgtgaaaa
      961 ggcagtcttc ccattaacac ataatcctcg actgttatat caggactttc aatgtcctgt
     1021 gtcactattg ctatattttg ggctctctct ttgtaagaga attttttaag atttttccca
     1081 tttaatttga ttgatccgct ctcaaatttt aaatctccgg ttatagccct gaataatgta
     1141 gtttttcctg atccattagg accaataatt ccaagaaagt taccttgttg tacactaata
     1201 cttatttcag atatcttgaa tttatttcca tatccacacg aaatattatc tattacaaaa
     1261 aaagcttcca ttttcttaaa atcatatatg tctcgtgttt ttgccgagat ttatcaatat
     1321 tgcaataaaa gatattccac caatgatacc ggtaataact cccaccggca attcgttagg
     1381 agctacaatt gttcgtgcaa tagtatcgca aaaaactata aatccaccgc cagtaagaaa
     1441 tgagctgatt agtaatattc tgaaatcaga gcctacaata agcctcataa attgaggtat
     1501 tatcaaccca acaaaaccta taacaccggc tatcgagaca cacatccccg tgagaagaga
     1561 agagataatg aacaggagtt taatagagag atctgtatta atgcccaagt gtcttgcttt
     1621 atcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacag gagtttaata
     1741 gagagatctg tattaatgcc caagtgtctt gctttatctt cacccagtcg gagagcattg
     1801 agaggatcga taaaaatata ggagattaaa agacaaagca tagaaactat tacagccacc
     1861 tttattaaat ccttgtttgt ttcatctaga gaacccatta cccaaaagac tatattatga
     1921 agattttcgt tggttgttgt tgccataata aacataattg ctgaagaggc tataaaactg
     1981 accattacac caatcaggag catgttttga gtatgaattt tgccttttcg gacactcaaa
     2041 atatacacaa gaaagacggt aattaaagcg ccaacgaaac cagaagcagg aagcataaaa
     2101 gaaccgactg aaaaattaag accggctact attgcgaatg caactccaag ggcggctcca
     2161 cctgaaattc ctagagtgta tggttcaacc agaggattcc gatatatacc ttgaagtatt
     2221 accccagcga gacttaatgc tcctcctaca gaaagtccaa gaataattct tggcagtcta
     2281 atttctttta aaactgtata ttcgagatcg ttatttctat taataagaat gttgtatatt
     2341 tttgacaaag gaatatctat ctctcctttt gcaagagaga atattactgt cattactgca
     2401 atcagcaaaa atgtaataag ccaaagtccc catttaatat acttagaacg gctcatttgt
     2461 aaagttttgt aacaagttct tcaacaacat cgacaaaagt cactggattt gggccacaag
     2521 acttgtaagg gtcaacagta aatatttttc cttttctggc agctgaaagt tgcttatatc
     2581 ttttccatac cgctttttct tcttcaccca ctatgcccat ggttactata acaatggcgt
     2641 ctggatcttt taatattaca ttttcacgtg taataattcc ggtgctaata tcagtggcta
     2701 tattaatgcc tcctgcctga gctataaagt catccataaa agtgtttgga acggcggtaa
     2761 aaagcggttt tgctccaagt tctatgaaaa ttttaggttt ttcccctttc ggaattgatt
     2821 ttttaatgct gtcgagcctt ctcttttggt attc
//
LOCUS       DAOTQL010000175         2851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27147_length_2851_cov_4.005722, whole genome shotgun sequence.
ACCESSION   DAOTQL010000175 DAOTQL010000000
VERSION     DAOTQL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27147_length_2851_cov_4.005722"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..875
                     /locus_tag="P5293_08160"
                     /pseudo
     CDS             1..875
                     /locus_tag="P5293_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
     gene            1233..1502
                     /locus_tag="P5293_08165"
     CDS             1233..1502
                     /locus_tag="P5293_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRR49907.1"
                     /translation="MITLEKAAKLIITDSGGVQKEAFFFKKPSLILRPETEWVEIVEC
                     GAAKLVDADYVKILAGFEEFKNKEIYFPQIFGDGHAAEHTCRSLL"
     gene            1679..2332
                     /locus_tag="P5293_08170"
     CDS             1679..2332
                     /locus_tag="P5293_08170"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRR49908.1"
                     /translation="MSWYVLYTSPRAEKRVAARLRNDGVEVFLPIHKKRRKWSDRMKI
                     VEVPLFNSYVFVRCSEQNLYSLLMIYGIKKIVYYLHRPAIVRECEIEAIKEFLKIAEN
                     KDIIYAGDQVEIVSGPFHQKCCKVLKVEKDRLILFLEELGAKIYVSLSEVDKLKSEGV
                     KSKSEGVKLKSEGVKLKSEDVKSKSEGVKSKSKGGELKPELESEKLQSEVGVLKTGS"
     gene            2405..>2851
                     /locus_tag="P5293_08175"
     CDS             2405..>2851
                     /locus_tag="P5293_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRR49909.1"
                     /translation="MDYNTQRPKLIMPEYGRNVQKMIEHVKNIKDRDKRNEQIRAVVA
                     VMGILNPQLRDLSDFRHKLWDHVYIISDFQIDIDSPYPVPTRETFVTKPNPIPLEKRP
                     LKAPHYGRNIQNMIEMVANKEEGEARDGMILVLANYMRQQYLIWNKD"
BASE COUNT          890 a          392 c          662 g          907 t
ORIGIN      
        1 atcattttca ggagagcata actgaggtta ttcttcatac agggcagcat tatgatcaga
       61 atatgtcaga ggtttttttt tcagagcttg gagttccgac accacactat aatcttaatg
      121 ttggttcagg ttctcacgga agacaaactg ctaaaatgat agaaggtatt gaaaatgtgt
      181 tgcttgcaga atcgcctgat gtggttttac tctttggcga tacaaattca actttggcgg
      241 gagcaattgc agcatctaag ttacacatcc cggttgctca tgttgaggct ggtttgcgtt
      301 ctttcaataa atcaatgcct gaggagatta acagaatcgt atgtgatcat gtatctactt
      361 ttctatttgt tccaactgag tcgggaataa aaaatctgaa gcgtgaaggt attggcgaaa
      421 agtttttcga aatccagagt gaaaagtcgc atgaagggtc agatgctgaa ggtgatacag
      481 gaaaagaaaa ttatcagaat tttaatgttg gaaatgttaa aaatagattt tcgattgaca
      541 atcctggaat ttttctcact ggagatgtaa tgtacgacaa ttctcttttt tttgctgatt
      601 tggcagatga gaaggcacca cttgagggta ttgttgagag aatcttaagt aactctttaa
      661 atgttaatat tgctaatatt tttgatcaaa agcacttgac aaagcaggtg agggtgacaa
      721 agaatcggtg gcagttaaca aaaaatcatt tcgttttagc tactgttcat agggatatca
      781 atacagataa tccgatgagg ttaaatgtca tatttaaggc acttgcagac attgctgaaa
      841 atattcctgt aattgttcca cttcatcctc gaactgcaaa gatgttaaaa agctctttgg
      901 aaacagctct ttttgagaga gtcgtcaagc ttaggcgaga gattgatact tttatgagcg
      961 acgctgacag gtttgatgat attgacaagt ttgatgatat tgacaagttt gatgatattg
     1021 acaggtttga tgatgataat gtaattggga cggataaaag tgatcatggg gatgtttctt
     1081 cttgtaatgt ttcttcttgt aatgagctta tttataagta tatgaataag tttgagtctg
     1141 aaacatttga gaatggaact gaaactcggg catttgactt tgaaactctt cgttctggag
     1201 tactgcttct acctccggca tctttttttg agatgattac tcttgaaaag gctgctaaac
     1261 ttataattac tgactcaggt ggtgtgcaga aagaggcttt cttttttaaa aaaccttctc
     1321 tgattcttcg gcctgaaact gaatgggttg agatagtgga atgtggtgct gccaaacttg
     1381 tggatgctga ttatgttaaa attttagcgg gttttgagga gtttaagaat aaagaaatat
     1441 attttcctca aatttttggg gatggccatg ctgctgagca cacctgtcgg agtttgttgt
     1501 aaaacgctga aaataaatga tgtaaaattt ggctctcaaa aagacgcaat ttttagacat
     1561 ctgtaaacca gcgttttaca acaaactccg acaggtacag ccacgacagg tgcagcccgg
     1621 tgcgacaggt gtgagttttt tttcttaaat tgcaataaat ttcagattaa ttagtgctat
     1681 gtcttggtat gttttatata cctctcccag agcggagaaa cgtgtggcag cacgtttaag
     1741 aaatgatggc gttgaggttt ttcttccgat tcacaagaag agaaggaaat ggagtgacag
     1801 aatgaaaata gttgaagttc cgctttttaa ctcttatgtt tttgtcaggt gttctgagca
     1861 gaatttgtat tctttattaa tgatatacgg cattaaaaag attgtgtatt atctgcatcg
     1921 tcctgctatt gttagagaat gtgagattga ggctattaag gaattcctga agattgccga
     1981 gaataaagat attatttatg ctggtgacca ggtggaaatt gtttctggtc cttttcatca
     2041 gaagtgttgt aaggttttga aagtagagaa ggatcggctg attctttttt tggaggaatt
     2101 aggtgctaaa atttatgtat ctttgtcgga agtcgataag ttaaaatcag aaggtgtaaa
     2161 atcaaaatct gaaggtgtaa agttaaaatc tgaaggtgta aagttaaaat cagaggatgt
     2221 aaaatcaaaa tctgaaggtg taaagtcaaa atcgaaaggt ggtgaattaa aaccggaatt
     2281 ggaaagtgaa aagttgcagt cggaggttgg agtgttgaag actggctcgt agtatagtta
     2341 aaaagatgta gtatagttaa aaagatggtt attaataagt ttagtgtgaa aaatacggat
     2401 aattatggat tataatactc aaaggcctaa gttgattatg ccggaatatg gcaggaatgt
     2461 tcagaagatg attgaacatg ttaagaatat taaggatagg gataagcgaa atgagcagat
     2521 aagagcggtt gtggctgtga tgggcatttt gaatcctcaa ctcagggatt tgagtgattt
     2581 taggcacaag ctttgggatc atgtttatat aatttctgat tttcaaatag atatagattc
     2641 tccttatccc gtgccaacaa gggaaacttt tgtaacaaag cccaacccta ttcctctgga
     2701 aaagaggcca ttaaaggcac ctcactatgg acgcaatatt cagaatatga ttgaaatggt
     2761 ggcaaacaag gaagagggtg aagcacgtga cggtatgatt ctggttttgg ccaattatat
     2821 gagacagcag tatcttatct ggaataagga c
//
LOCUS       DAOTQL010000176         2840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27290_length_2840_cov_3.526032, whole genome shotgun sequence.
ACCESSION   DAOTQL010000176 DAOTQL010000000
VERSION     DAOTQL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27290_length_2840_cov_3.526032"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="P5293_08180"
     CDS             complement(<1..525)
                     /locus_tag="P5293_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRR49910.1"
                     /translation="MKLDSELLSITVLMDDSDNSVREAVIERLIARGEEALSVLEQFV
                     AENKLNISSYQQLITEVKERIAFNKFLNYLNSKDHSLIDGLVLLTKCVDPQWDERNFL
                     FVVSEMSDDIIKEFSDDNTDFEKLNIFNYCFFKRFGFNISLTDASEEWREIDKVIEKR
                     DGSSITVSIIYFLLA"
     gene            complement(534..1409)
                     /locus_tag="P5293_08185"
     CDS             complement(534..1409)
                     /locus_tag="P5293_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRR49911.1"
                     /translation="MKPIAPSELILNSDGSVFHLHLRPEELADNVILVGDPGRVEMIS
                     SFFETKEIVRSSREFNTVTGKYKGKRITVLSTGIGTDNIDIVMNEIDALVNIDLEKRV
                     VKDEHKSLNILRIGTSGAIQPDIPLGSFVLSHISIGCDGLLNWYSGRDRVCDLDMERE
                     FVERTGWISNLPAPYFIHSSDKMINILKDETIEGVTISASGFYGPQGRVLRIPLAMPD
                     MLEQFENFRYHGRRITNFEMESSAIAGLSAILGHNATTVCCIIANRHRKESRADYSPD
                     IKRLVELSLNRIIQL"
     gene            1476..1907
                     /locus_tag="P5293_08190"
     CDS             1476..1907
                     /locus_tag="P5293_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HRR49912.1"
                     /translation="MIQRVQTLFLLAVTLLMGSQFFSVMVYSPSEKVKYLAFFPCIIF
                     IVITTLISFFTIFLYHHRMLQIRITIFNTVLLIGYQIWLMYYFFKRPEGTAFSWTAIF
                     PAVSAILSILAIRYIARDEALLRTAKHLRSVEKNRNKIKEK"
     gene            complement(1912..>2840)
                     /locus_tag="P5293_08195"
     CDS             complement(1912..>2840)
                     /locus_tag="P5293_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR49913.1"
                     /translation="IAQSKIKAYRSMASDSIKVSAFADTTQKYNKLLALDADFAKKEF
                     DNNLLQYRDVDITLKPMYKFMATKAIEEYAIVLNRGYEDSYLNDFVSRIPIPVRISSA
                     PVELSGEQQHIYMETIDNLIRENRNGYILFAKALLESNMKKFNLALDFYNQAITTEPD
                     QTFFYINRGALQAEMIDFISSIESNVQVLTLDDAGATRAKVQGKSSGNYDYSAAIQDM
                     KKAASLSPNFPFIYYNLGNLYCLSNNLPESINNYTKALEIYPYIAEAYYNRGLVLIYL
                     KDREKGCYDLSKAGELGLREAYAVIKKYCSEE"
BASE COUNT          915 a          559 c          427 g          939 t
ORIGIN      
        1 ggctaataaa aaataaataa tagatacagt tatagaactc ccatctcttt tttctatgac
       61 tttgtcaatt tctctccact cttcagatgc atcagtcaaa gagatattaa atccaaaacg
      121 tttgaaaaaa caataattga aaatatttag cttttcaaag tcggtattat catctgaaaa
      181 ctctttaatt atgtcatccg acatctctga gacaacaaaa agaaaattac gttcgtccca
      241 ttgaggatca acacacttag tcagaagaac aagaccatca atcaaagaat gatctttaga
      301 atttaaataa ttaagaaact tattgaaggc tattctctcc tttacttctg taatcaactg
      361 ttgatatgat gaaatattca gtttattttc agcaacaaac tgttccaaaa cagacaaagc
      421 ctcctcacct cttgctatta atctttcaat aacagcttca cgaacagaat tgtccgaatc
      481 atccattaaa acagttatcg aaagaagttc cgagtcaagt ttcatcacaa ttgttacaat
      541 tgaataattc tgttcaagga cagctctaca agcctcttta tatctgggct gtaatctgct
      601 ctgctctctt ttctatgccg attggcaata atacagcaaa cagtagtagc gttatgaccc
      661 aaaattgcag acagacctgc tatcgccgaa ctctccatct caaagtttgt aattcttctg
      721 ccatgatacc tgaaattctc aaattgttcc aacatgtcag gcatagcaag agggattctc
      781 aaaactcttc cctggggacc ataaaatcct gaagcggata tggttactcc ttcaattgtt
      841 tcatccttca aaatgttaat catcttatca cttgaatgaa tgaaataggg tgcaggtaga
      901 ttactaatcc atccagtacg ttcaacaaac tcacgttcca tatccaaatc acacactctg
      961 tctcttcctg agtaccagtt taaaagccca tcacacccaa tagaaatatg tgaaagcaca
     1021 aatgacccaa ggggaatatc aggttggata gctccagaag tacctatacg aaggatattc
     1081 agacttttat gttcgtcttt cacaactctt ttttccaaat caatattgac aagagcatct
     1141 atctcattca tgacaatatc aatattgtca gtacctatac cagtagacaa gacagttatt
     1201 cttttcccct tatattttcc ggtaacagta ttaaactctc ttgaagatct aacaatctct
     1261 ttagtctcaa aaaaagaaga aatcatttct actcgtcccg gatctccgac aagaattaca
     1321 ttgtcagcca attcttctgg tctgagatgt aaatgaaaaa ctgatccgtc actatttaat
     1381 atcaattctg aaggggcaat aggtttcatg tttaattaat taatcattta caaaaataac
     1441 cttatatttt atatttttgt aaaaatttac gattcatgat acaaagagtt caaacactat
     1501 ttctgcttgc tgtcacatta ctgatgggct cccaattttt ctccgttatg gtttattccc
     1561 cttctgaaaa agtcaaatat cttgcatttt ttccctgtat tatctttata gttataacta
     1621 ctctgatatc tttctttaca atttttctct atcatcacag gatgcttcag atcagaatta
     1681 caatcttcaa cactgtgctt ctgataggat atcaaatatg gttaatgtat tattttttta
     1741 aacgtcctga aggaactgct tttagctgga cagctatatt tcctgctgta tcagcaatac
     1801 tttcgatact tgcaatccgt tacatagcga gagatgaggc ccttttacga acagccaaac
     1861 atttgagaag tgttgaaaaa aacagaaaca aaataaaaga aaaataactg attattcctc
     1921 cgaacaatat ttctttatta ctgcatatgc ctctctgagc cctaattctc cggccttact
     1981 caggtcataa cacccttttt ctctgtcttt gagataaata agcacaagac ctctgttata
     2041 ataggcttca gcaatatatg gatagatttc cagggctttt gtataattat tgatcgattc
     2101 aggtaaattg ttcgacagac agtataaatt accgagattg taatatatga atggaaagtt
     2161 tggtgacaag gatgcagctt tcttcatgtc ctgaattgct gccgaataat cataattacc
     2221 agaagatttt ccctgcactt tagctcttgt tgcacctgca tcatcaagtg taagcacttg
     2281 cacatttgat tcaattgaag atataaaatc aatcatttct gcttgtaagg ctcccctgtt
     2341 tatatagaaa aaagtctgat caggttcagt tgtaattgcc tgattataaa aatcaagtgc
     2401 aaggttgaat ttcttcatgt tagactcaag aagagctttg gcaaataata tatatccgtt
     2461 tctgttttct ctaattaagt tatcaattgt ttccatgtag atatgctgtt gttccccgga
     2521 caattctaca ggggcagaag aaatccggac aggtatagga attcttgaaa caaaatcatt
     2581 gagataagaa tcttcatagc ctctgttaag tactattgca tactcttcta tagctttagt
     2641 cgccataaat ttatacatag gcttgagtgt tatatcgaca tctctatact gaagaagatt
     2701 gttatcaaac tctttctttg caaaatcagc atcaagtgca agaagcttgt tatatttctg
     2761 agttgtatcg gcaaatgctg aaaccttgat agaatctgaa gccattgatc tgtaggcctt
     2821 aatttttgat tgagcaattt
//
LOCUS       DAOTQL010000177         2807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27742_length_2807_cov_3.534157, whole genome shotgun sequence.
ACCESSION   DAOTQL010000177 DAOTQL010000000
VERSION     DAOTQL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27742_length_2807_cov_3.534157"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="P5293_08200"
     CDS             complement(<1..377)
                     /locus_tag="P5293_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49914.1"
                     /translation="MKITLTTLLALLPIFSAAQNFSNLNFEQAKSVAEKEKKIILVDV
                     TSKRMLNDQKFKSEKLLASDKGVADFCSNNLVAIRIDMETQEGKEFAPLLQMNMYPTY
                     AFMMPNGDLLGVIHPSRIYNNPTL"
     gene            complement(393..1907)
                     /locus_tag="P5293_08205"
     CDS             complement(393..1907)
                     /locus_tag="P5293_08205"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR49915.1"
                     /translation="MKKILNFIVIAFTLTITFSCNLDLAPENVIVDEITYKDSSTAEA
                     ALIGAYTRLNASISGAPTGVNNYANSGYFILFSEIGTPTLTVRSNSSFVNMVTSQYNS
                     SDHDGFIQSIWGTTYNAIDYANNVIANIEKYGHYSTSAMKRHIAEAKFIRAYEYLVLL
                     QAYGDGALTGDMNGLGVIMRLKPYDGYNPKDIQARVTVGETYNQIITDLNEALNDLPN
                     GTANTLVSRTRASKTAAFALLSRVYLYRGTYKNDLQDMQMAANYADSVINNTKGFNFS
                     TSYTEHANNMFPLNSTGTETNSTNYSSEVILLSPCYSSANNYSNGLGSYFYNKSYFYV
                     DLDFAGIYAAGDRRGYIDPATATSLLWYGSSTSNPNDLTSYKYNNGGGYNNVIYLRVS
                     EAKLTKAEALARINGINDVSIAQLNDIRRRPFSTKPAALTAANFATPQALIDEILLER
                     MKELAFEGHTRWDLIRTGRALRDPSVTNDRKILPIPNYDINISYGVIIQNKGYR"
     gene            complement(1926..>2807)
                     /locus_tag="P5293_08210"
     CDS             complement(1926..>2807)
                     /locus_tag="P5293_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49916.1"
                     /translation="KQDQNIGDMSNSGIELRFTSYNISGKDFQWMTTLTLSRNTNKIL
                     KLNFEGNQIDELNSSYKYYAVGHSAAQFYLHKWYGVNPESGNPLWVYKDGTISETPPA
                     ADNSTAPYNKFIMGDATPDFYGGFNNSFIYKGFEVNFLFTFSYGGQMINNTKAQLMTY
                     STKDANNLDKDILKFWQIYGHKTDVPKLKNASITGNYDYTASSTITRFLEDNSYLRLK
                     NLEIAYSLPENLLQKYKLFKQVRIYAIATNLLTFTKYSGIDPEVSAFGSSATASGYDN
                     LTMPQTRAFQFGVKLGF"
BASE COUNT          778 a          497 c          563 g          969 t
ORIGIN      
        1 agagtgggat tattgtaaat gcgtgaagga tgtattacgc caagaagatc gccgtttggc
       61 atcataaacg cataggttgg gtacatattc atttgtaaca gaggagcgaa ttcctttccc
      121 tcttgtgttt ccatatctat tctgattgca actaagttgt ttgagcagaa atcagcaaca
      181 cctttgtccg aagccagcaa tttttctgac ttaaactttt gatcgtttaa cattcttttg
      241 ctggtaacat caactaaaat tatttttttt tctttttcgg caactgattt ggcctgttca
      301 aaattaagat tagaaaaatt ctgggcagcc gaaaaaatag gcagcaatgc gagtagtgtt
      361 gtaagagtaa ttttcataca aatatttgtt tattaacgat atcctttatt ttgaattatg
      421 actccatagg atatgttgat gtcataattt ggtattggca atatttttct gtcatttgtg
      481 accgaagggt ccctaagtgc tcttccggtt ctaataagat cccatcttgt gtggccttca
      541 aatgcaagtt ctttcatcct ttcgagtaat atttcatcta tgagagcctg aggagtagca
      601 aaattagcgg ctgtgagtgc tgcaggtttt gtagagaatg gacgtcgtct tatatcgttc
      661 aattgagcaa ttgagacgtc attgatgccg tttattctgg caagagcctc cgcctttgta
      721 agcttggcct ctgatactct taaatatatt acattattat acccaccgcc attgttgtat
      781 ttgtaggaag tcaagtcatt tggatttgaa gttgagctgc cataccacaa caaagaagtt
      841 gctgttgcag gatctatata gcctcttctg tcgcctgcag cgtagatgcc tgcaaaatca
      901 aggtccacat agaaataact tttgttataa aagtaactgc ctaaaccgtt tgaatagtta
      961 ttggcacttg agtaacatgg tgaaaggaga ataacttccg aactgtagtt tgtgctgttt
     1021 gtctcagttc ctgtgctgtt gagtggaaac atgttattag catgttctgt gtaagatgta
     1081 gaaaaattaa accctttggt attatttatt accgaatctg cataatttgc tgccatctgc
     1141 atatcctgaa gatcattttt gtaagttcct ctgtaaagat agactcttga cagcaaggca
     1201 aaagcggcag tttttgaagc cctcgttctt gacacaagtg tatttgctgt gccatttgga
     1261 aggtcattga gagcttcatt gagatctgtg ataatctggt tataggtttc acctacagta
     1321 actcttgcct gaatatcttt tggattgtaa ccatcatatg gtttcagtct cattattact
     1381 ccaagtccgt tcatgtctcc tgtgagggca ccgtctccat atgcctgaag cagaactaga
     1441 tattcatatg ctctaatgaa ttttgcttct gcaatatgtc tcttcattgc tgaagtgctg
     1501 tagtgaccat atttttcaat attggcaatt acattgtttg cataatcaat tgcattgtaa
     1561 gtagttcccc agatgctctg aatgaatcca tcgtggtcgg aactgttata ttgagaagtg
     1621 accatattta caaatgagga attgcttctg acagtcagcg ttggtgtgcc aatttcgctg
     1681 aaaagaatga aatatcctga atttgcgtag ttgttgacac ctgtgggagc tccggagata
     1741 ctggcgttga gtcttgtgta ggctccaata agagctgctt ctgccgtact ggagtccttg
     1801 tatgtaattt catctacaat tacattttca ggagcaagat ctaagttgca ggaaaatgtg
     1861 attgtcaatg tgaatgcaat tactataaaa tttaatatct ttttcataat catcaattta
     1921 gtcatttaaa aacccaattt cacaccaaat tgaaatgccc tggtttgagg catggtcagg
     1981 ttatcatacc ctgacgcggt tgccgaagaa ccgaatgcac ttacttcagg atcaataccg
     2041 gagtatttgg tgaatgtcaa aagatttgtt gcaattgcat atattctcac ctgtttgaat
     2101 aatttgtatt tctgaagcaa attttcagga agagagtatg caatctcaag attcttcagt
     2161 ctcaaatatg agttatcttc aaggaaacga gtaatagtac ttgaagcagt ataatcatag
     2221 tttcctgtta tagatgcgtt cttaagtttt ggaacatctg tcttgtggcc gtatatctgc
     2281 cagaatttca aaatgtcctt gtcaagattg ttggcatctt ttgtagagta ggtcattagc
     2341 tgcgcttttg tattatttat catttgacct ccataagaaa atgtgaagag gaaatttact
     2401 tcaaaaccct tgtatataaa agagttgtta aatcctccat agaaatcggg agttgcatca
     2461 cccattataa acttattgta gggagctgta gaattgtctg cagccggagg agtttcactt
     2521 attgtaccat ctttgtatac ccacaatgga tttccgcttt caggatttac tccataccat
     2581 ttatgtaaat agaattgagc tgccgaatga cctactgcat aatacttgta tgaactgttg
     2641 agttcatcaa tctgattccc ttcgaaattg agctttaata ttttgtttgt gtttcttgag
     2701 agtgtaagtg tagtcatcca ttggaaatcc ttgccagaga tattgtaaga agtgaacctg
     2761 agttcaattc ccgagttaga catatcaccg atgttttggt cttgttt
//
LOCUS       DAOTQL010000178         2806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27750_length_2806_cov_4.111959, whole genome shotgun sequence.
ACCESSION   DAOTQL010000178 DAOTQL010000000
VERSION     DAOTQL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27750_length_2806_cov_4.111959"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="P5293_08215"
     CDS             <1..663
                     /locus_tag="P5293_08215"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRR49917.1"
                     /translation="ILLTIIFLVAIACLLIFTELIYRRYELKGEMTRKFAHFAATLST
                     TVFPFLFNSHWYVLILATIFFIVLLISKQIKQLQSINDIDRKSTGSYLLPVSIYLTFL
                     ISDLLDSKIMFILPMLVLAICDPMAGILGLNFKRRNRNIRIFGHTLHKTVLGSGSFFV
                     SCFIICILALYFNNMDFNFKTFWLSACIASVSTLVEMFSWRGTDNLLIPLSVLFMLVI
                     FL"
     gene            699..1769
                     /locus_tag="P5293_08220"
     CDS             699..1769
                     /locus_tag="P5293_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR49918.1"
                     /translation="MNRIIIDGANGYVASHFAAELLKNNNQVLALARSNGDLSPSMRM
                     KKILDEINENREDDYKNLKVYNYSLLDKDFSLTKDELKEIFREKSDFFHFAASLKFAE
                     KDKKEIFETNVEGLENAISLFLNYSLPGSRFFYISTAYSCGNNGNLFMEQFYPNEDIS
                     AFRNYYEQSKRYGENIVKKYIDENSLNGYVLRLSQVAGNNITGVTTTDYGIFDFVKRL
                     QSFSRRYPGETVRISATPESTQNIIAIDKIVNYLITLVSIDKLPTIINLVARNYITND
                     EIIKCICRLLPINILQVRELNPDSMNILERIIAAGMSFTGKYSNTNLNFDTTNIDLLN
                     FPRDNEITPDSLCRMIEYVIKH"
     gene            2166..2366
                     /locus_tag="P5293_08225"
     CDS             2166..2366
                     /locus_tag="P5293_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49919.1"
                     /translation="MTKSQVFDCALMAVGITAIGDLISSGINGMSKSAVKKVLKKVAV
                     RYMGWVGAGIALYEFGDCMSWW"
     gene            complement(2748..2805)
                     /locus_tag="P5293_08230"
     tRNA            complement(2748..2805)
                     /locus_tag="P5293_08230"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2778..2780),aa:Met,seq:cat)
BASE COUNT          942 a          429 c          483 g          952 t
ORIGIN      
        1 atattattaa caatcatttt cctcgttgca attgcctgtt tgctgatttt tacagaattg
       61 atatatagaa gatacgaact gaaaggtgag atgacgagaa aatttgctca ctttgcagca
      121 acactttcta caactgtatt cccctttctt tttaacagtc actggtatgt tctgattctg
      181 gcaacgattt tctttatcgt gttgcttatc agcaaacaaa tcaaacaact tcaatcaata
      241 aacgacatag atagaaaatc gacgggaagt tatctgcttc cagtatcaat atatttgact
      301 ttcctgatat cagatctatt agacagcaaa attatgttta ttcttccaat gcttgttctt
      361 gccatctgtg acccgatggc gggtattctt ggccttaatt ttaaaaggag aaacagaaat
      421 atcaggattt ttggtcatac cttacataaa actgttcttg gatcaggatc tttctttgtt
      481 tcatgcttca taatatgcat tttagctttg tatttcaata atatggactt taattttaag
      541 acattttggt tatcagcatg tatcgcttca gtgagtactt tagtagagat gttcagctgg
      601 agaggtactg acaatttgtt gattccacta agtgttttgt ttatgcttgt aatttttctt
      661 taatttataa ctctgttttg atagataaac aataaaacat gaatagaata atcatagatg
      721 gagcaaatgg atatgtggca tcacattttg cagcggaatt acttaaaaac aacaatcagg
      781 tacttgcatt agcaagaagc aacggagact tgtcaccttc aatgaggatg aaaaaaattt
      841 tagatgaaat aaatgaaaat agggaagatg actacaaaaa tctaaaagtt tataattact
      901 ctttgctgga taaagatttt tctcttacta aagatgaatt aaaggagatc ttccgtgaaa
      961 agtctgattt ttttcatttt gcagccagtc ttaaatttgc ggaaaaagat aaaaaggaga
     1021 tttttgaaac taatgtagag ggtctcgaga atgcaatctc tttatttcta aactattctt
     1081 tgcccggctc cagatttttt tacatcagca cagcctattc atgtggaaat aacggaaacc
     1141 tctttatgga gcaattctac cccaatgaag acatttctgc ttttcgtaac tattatgaac
     1201 aatcaaaaag atatggtgaa aacattgtta aaaaatatat agatgagaac agccttaatg
     1261 gatatgtgtt gagactgtca caagttgcag gaaataatat tacaggtgtt acaacaacag
     1321 attacggaat attcgatttt gtcaagagac tccagtcttt ttcaagaaga taccctggtg
     1381 agaccgttag aatatcagca actcctgagt ctacacaaaa cattatagct attgataaaa
     1441 tcgtcaacta tcttatcact cttgtctcaa tcgacaaatt gcccactata atcaatcttg
     1501 tggcaagaaa ctatattact aatgacgaaa taattaaatg catatgcaga ttattaccaa
     1561 tcaatattct gcaagtgaga gaacttaatc ctgactcgat gaatattctt gaaaggatca
     1621 ttgctgcagg gatgtcattt acaggcaaat actcaaatac aaatctgaat tttgatacaa
     1681 ctaatattga cctgctcaat tttccaaggg acaatgaaat tacacccgac tcactatgca
     1741 gaatgattga atatgtgata aaacactaag tcatctattt tccttacctg ttcctttgtc
     1801 tatggcaaga atcaagtaat ctatcttact tcttgcatct caaaatctgt aatcaaatca
     1861 ggacaacacc tcatcatgag gtttgaatat gagtattgat tatcttctga aactgtcctg
     1921 tttttaggga atgctacaac ttcagtgttg tttggcggtt atataattgc ttaatcaatg
     1981 taattttttt ataaaacaac ttgtaaagta ttgattgtaa gaaaaaaaag ctaactttat
     2041 acgaaaacat tattttaatg gctgtattgg actatccatc cctttatgtg cctaaacatt
     2101 cagctgcgta ttatgtgtta taagcagaat ggtataagtt ctattaatta gtaaaattga
     2161 attttatgac aaaaagtcaa gttttcgatt gtgctttaat ggctgtgggc attacagcta
     2221 taggagactt aatatcaagt ggaataaatg gtatgagtaa atctgctgta aaaaaagttt
     2281 taaaaaaagt tgctgtaaga tatatggggt gggttggagc tggtattgct ttatatgaat
     2341 ttggtgattg tatgtcctgg tggtaattat atatatattg atatgaaaaa tattcctttt
     2401 gtactattgg ctattatatt attaatagtg atgatactag aaatagtaaa attagatttt
     2461 tccggtttgg acggtaatga tatttttaaa atgacactgt tatttgtctc ttttataata
     2521 gtgattttca tgtttgttaa aagtttagca aaaaaaggaa aagataagta gtagagttat
     2581 agaagaactg tcctgtcctg aaaaccgtta caggtttgac aatctaaaat aagtaaaaat
     2641 ttgaacttct aaatatcgtt taagaactac ttgaatgatg ccctattaat cataaataaa
     2701 gcccacaact aatgatatat caaatagttg tgggcttttg tagcggagga aggactcgaa
     2761 cctacgacct tcgggttatg agcccgacga gctaccaact gctcca
//
LOCUS       DAOTQL010000179         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27860_length_2798_cov_5.805323, whole genome shotgun sequence.
ACCESSION   DAOTQL010000179 DAOTQL010000000
VERSION     DAOTQL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27860_length_2798_cov_5.805323"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="P5293_08235"
     CDS             <1..287
                     /locus_tag="P5293_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49920.1"
                     /translation="EDRKRKERKETLNQLAITALIGIGMVTILLIIRYFLQDLSLSIL
                     TVTNDLKSSFSIFANFSNNPGFKEWIIIGANLAFLLTIEQIVSKKMKRKD"
     gene            complement(284..1762)
                     /locus_tag="P5293_08240"
     CDS             complement(284..1762)
                     /locus_tag="P5293_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HRR49921.1"
                     /translation="MFNNEDVEYIKQHGSSVEKVLREIECFKSGFPFLKIVSPATASR
                     GIRVLNQEEQSEAEKRYLNYDGVPCKFVPASGAASRMFKDLFDAKKELELGNDINGTS
                     AELFFTNIEKFAFYDDLTKLKGFDKSNPLKVLDFLLSNSGLNYGNLPKGLIKFHKYSD
                     GSRTAFEEHLVEAALYARQKSGEASLTITVSPEHLEAFKKHFEEVKFKYFERFKTSYR
                     VNFTLQKPDSDTIAVDLDNNPFRSNDGKLLFRPGGHGALIGNLNDLDSDIVFIKNIDN
                     VVREQYIDETVKWKRILAGVLIEMQNKIFNYLKLLDEEFNEALNSEIISFLEKELCIT
                     IPSLPEPIVREYLREKLDRPIRVCGMVKNLGEPGGGPYIVYDADGSTSLQIVESAQLN
                     MLDPQVARIVENSTHFNPVDIVCSLKNYQGERYDLYKYVDSETGFISYKSYQGRSLKA
                     LELPGLWNGSMSQWNTVFVEVPLITFNPVKSINDLLRKEHLA"
     gene            complement(1755..>2798)
                     /locus_tag="P5293_08245"
     CDS             complement(1755..>2798)
                     /locus_tag="P5293_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HRR49922.1"
                     /translation="SSSDLVDVDAMIIRTRTACNESLLHGSSVKFIASATIGKDHVDM
                     DYCKNNNIYFTNAAGCNAWGVVQYVLTSIFTVSHIKHFNVCGKTIGIIGAGNVGERLA
                     WILELLGFKIMRCDPPVRDILVSGQIPEGDLLFSYRSSLKPSDYYSIEEVVKNSDIVS
                     LHLPLDCNTFAMLNDECFSLMKPGCIFINSSRGEVLDEEALVSHSDKLGAVIIDVWSN
                     EPNISLPLLSVADIATPHIAGYSLEGKINATVMSVNALGIFFGIESLSNFRIEYPPYI
                     RMSSLDDKIDDVQEWFFRILYESFPVYETDLLLRKEPLSFEKIRSEYVYRREIPENLY
                     KEIANKIKFNSYV"
BASE COUNT          927 a          557 c          412 g          902 t
ORIGIN      
        1 ttgaggatag aaaaagaaaa gaacgcaaag aaactctcaa tcaattggcg attacggcac
       61 ttattggtat tggtatggtg actatattac tgattattag atattttctc caagatttaa
      121 gcctgtcaat cttaacagta acaaatgatt taaagagcag tttttcaatt tttgccaatt
      181 tttctaataa tccaggattc aaggaatgga ttatcattgg tgcaaatctt gcatttctac
      241 ttactataga acagattgtt tcaaaaaaaa tgaagagaaa agactaggca agatgttctt
      301 ttcttaaaag gtcatttata ctctttacag gattaaatgt aatcaagggg acttcaacaa
      361 aaacagtatt ccactgactc atgcttccat tccacaatcc aggcagttct aaagccttca
      421 agctacgccc ttgatagctt ttataagaaa taaacccagt ctcagaatca acatacttgt
      481 aaagatcata tctttctccc tgataattct ttaatgagca gactatatct accggattga
      541 aatgagttga attttcaaca atcctggcaa cttgaggatc cagcatgttt aattgagccg
      601 attcaactat ttgtaatgat gtagagccat ctgcatcata gacgatataa ggtccgccac
      661 caggttcacc aaggtttttt accattccac atactctaat gggtctgtcc agtttttctc
      721 tcagatattc ccgaactatt ggttctggaa gagaaggaat ggttatacaa agttcttttt
      781 caagaaatga gattatctca ctgttgagag cctcattaaa ttcttcatcc aaaagtttca
      841 aataattgaa aattttattt tgcatttcaa taagaacacc tgccaatatc ctcttccatt
      901 ttactgtctc atcaatatac tgctctctaa caacattatc tatattttta ataaatacta
      961 tgtccgaatc taaatcattc aaatttccaa taagagcacc atgtccaccc ggcctaaaga
     1021 gtaactttcc atcatttgat ctgaagggat tgttgtccaa atctacagca atagtgtctg
     1081 aatcaggctt ttgaagagta aagttaaccc tgtaacttgt cttgaatctt tcgaaatact
     1141 taaattttac ctcttcaaag tgttttttaa aagcttcaag atgttcaggt gaaacggtaa
     1201 tagtaagaga tgcttctcca ctcttttgtc tcgcataaag tgcagcctcc accaaatgct
     1261 cttcaaaagc tgtccttgat ccatcagaat atttatgaaa ttttatcaaa cctttaggca
     1321 agttgccata attaagtcca gagttactta atagaaaatc cagcactttt aaaggatttg
     1381 atttgtcaaa tcctttaagt ttagtcaaat catcataaaa tgcgaatttt tcgatatttg
     1441 taaaaaacag ctctgcagat gtgccattaa tatcattccc aagttccaat tctttttttg
     1501 catcaaacag atctttaaac attcttgaag cagcaccgga agccggaaca aatttgcaag
     1561 gaacaccatc ataattgagg tatctttttt ctgcttcaga ctgttcctcc tgattgagga
     1621 ctcttatacc tctactagct gtagcaggtg aaacaatttt caaaaaaggg aaccctgatt
     1681 taaaacattc tatctctctc aaaactttct caacactgga accatgttgc ttaatatatt
     1741 ctacatcttc attattaaac atatgaattg aattttattt tatttgctat ttccttatat
     1801 aaattttccg gaatctctct cctataaaca tactcacttc tgattttttc aaatgacaaa
     1861 ggctcctttc taagaagtaa atctgtttca tagacaggaa aagattcata taatattcta
     1921 aaaaaccatt cctgaacatc atcaatttta tcatccagac ttgacattcg tatatatggc
     1981 ggatactcta ttctgaagtt ggataaactt tctattccaa aaaaaatacc taaagcattc
     2041 acagacatta cagtagcatt gattttccct tcaagtgaat atcctgctat gtgtggagtg
     2101 gcaatatctg ctactgaaag caatggcagg ctaatattcg gttcatttga ccagacgtca
     2161 ataatcactg cccctaattt atcagaatgc gaaacaagtg cctcttcatc caatacttct
     2221 ccccttgagg aattaatgaa aatacaaccc ggtttcatta gtgaaaaaca ctcatcgttc
     2281 aacatcgcaa aagtgttaca atccaaaggc aagtgtaaag aaacaatatc tgaattttta
     2341 acaacctcct caatcgaata ataatcagaa ggcttaagag aacttctgta agagaatagt
     2401 aagtctcctt ccggaatctg ccctgatacc agaatatctc taaccggcgg atcacaccgc
     2461 atgattttga aacctaatag ttcaagaatc catgccaatc tttctcctac atttcctgcg
     2521 ccaataatac ctattgtttt accacaaaca ttaaaatgtt ttatatgcga tactgtgaaa
     2581 atagatgtaa gtacatattg aaccacaccc caagcattac aaccggcggc attggtaaaa
     2641 taaatattat tgtttttaca gtagtccata tctacatgat ctttaccaat agtagctgag
     2701 gcaataaatt taacagagct tccatgaaga agagattcat tgcatgctgt tcttgtcctt
     2761 atgatcatgg catcgacatc aaccagatct gaagaaga
//
LOCUS       DAOTQL010000180         2791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_27953_length_2791_cov_4.653509, whole genome shotgun sequence.
ACCESSION   DAOTQL010000180 DAOTQL010000000
VERSION     DAOTQL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27953_length_2791_cov_4.653509"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="P5293_08250"
     CDS             complement(<1..530)
                     /locus_tag="P5293_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRR49923.1"
                     /translation="MSIFKIKQTSQTIKNVDKFFDIIDKGLLVFKEGMRNYLYANTTL
                     FEENLSEMAKLESEANYLRREIVASVHTMSFMSQMRGDILKLLERMDNIVDLLNNSLS
                     QLDIEIPHIPSELDADFIKLTELSTMAVESVIPAARAYFRNPDSIQEKINRVYFYEEE
                     TERQSKTLKRKVFHEMP"
     gene            830..1639
                     /locus_tag="P5293_08255"
     CDS             830..1639
                     /locus_tag="P5293_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HRR49924.1"
                     /translation="MIVGIDIGSTTTKAVAVKDGVIVQTVKTRAFDAVTSATGAFGKM
                     IMESKTKILDVEKIIVTGAGSLRINSEIFGIPTSKINEIEAIGIGGMFLSKSDNIIIT
                     NIGTGTSVIEATKEKITHLGGTGVGGGTIIGLAKELIKTQTFDNILNLADKGDLTQVD
                     LLIEDISDLEISFLDRKATAANFGKMLDTARKEDIALAIINLVYQVIGVISMFAAKSR
                     GNDNVLITGNGSKNRIGRKILEEVSRMYGINFIFPAEAEFATAIGAALSQS"
     gene            complement(1914..2315)
                     /locus_tag="P5293_08260"
     CDS             complement(1914..2315)
                     /locus_tag="P5293_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49925.1"
                     /translation="MKTLTLSIKQTEFEEIASGKKRIVTRDIKPSNSHKYIGFEDLIS
                     HTKYTDWKAIPNGTQDIYIQPIKYDYIIIKPSKSADGKECPKLCFKVNDTEVLCLMDE
                     NEDQIYKVEKGQEYPAMCIDYFLGEILNVDE"
     gene            complement(2403..>2791)
                     /locus_tag="P5293_08265"
     CDS             complement(2403..>2791)
                     /locus_tag="P5293_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49926.1"
                     /translation="IGILDMSEYSKELKIVQRSNYWSKNDIYNFKLLTDTEKTFATWI
                     VGPPIKDFLNNKGQPALLCEYVITYPDKSKEKFGPFGFFEPGFSILIINPSTKLIGKW
                     KIDYYIIDRDTGERRHAATRFFTLSE"
BASE COUNT          922 a          417 c          497 g          955 t
ORIGIN      
        1 ggcatctcat gaaatacttt tctttttaaa gtttttgatt gtcgctcggt ctcttcttca
       61 taaaaataga ctctgtttat tttttcctga atggaatcag ggtttctaaa atatgccctt
      121 gctgctggaa taacactttc tacagccata gttgaaagtt cggtgagctt aataaaatca
      181 gcatctaatt ctgatggtat atgtggaatt tcaatgtcca gctgtgaaag gctgttattt
      241 aacaaatcga cgatattatc catcctttct aacaacttga gaatatcccc tctcatctgt
      301 gacataaaag acatggtatg aacagaagcc acaatctctc gtctgagata attagcctca
      361 ctttcaagtt ttgccatctc tgaaagattc tcttcaaaaa gagttgtatt agcatataag
      421 tagttcctca taccctcttt gaaaaccaac aatcctttat cgattatatc aaaaaatttg
      481 tcgacatttt taattgtctg actagtctgt tttattttaa aaatactcat agctcgtcta
      541 aaaagtatct ttttcaaaga tatataaaaa tagtttttgt gtatttaggg gattttaaac
      601 aaaatgtttt aaataattta tgagagttat aacatgaatt aaataacgta aaaccaaata
      661 tataaattta tcttgtagtg gaaattaact tacgatagaa aataatgtag tgttttttta
      721 taattgcatt tattgttttt ttaaaatctg aagagttttt ataacactac atagacggaa
      781 aattatttta aacattaaat ttgaatcaga attcaataat ataattataa tgattgttgg
      841 tattgatatt ggcagtacga ctacaaaagc tgtggctgtg aaggatggcg taattgttca
      901 gactgtaaag acacgagctt ttgatgctgt cacttctgca acaggtgctt ttggaaaaat
      961 gattatggag agtaagacta aaattttgga tgtcgagaaa ataatagtca cgggagccgg
     1021 ttcgttgagg attaattctg aaatttttgg tattccaact tcaaaaataa atgagataga
     1081 ggcaattggt ataggtggca tgtttttatc caaaagtgat aatatcataa ttactaatat
     1141 aggaacaggg acctcagtaa ttgaggcaac taaagaaaaa attacacatc ttggcggaac
     1201 aggagttgga gggggtacca ttattggact tgccaaagag ttgattaaga cacaaacttt
     1261 tgataatatt cttaatctgg cagacaaggg agatcttact caagttgatt tacttatcga
     1321 agatatatct gatctcgaaa ttagctttct tgacagaaaa gctactgctg ccaattttgg
     1381 aaaaatgctt gatactgctc gtaaagaaga tatcgcactg gctataatta atttggttta
     1441 tcaggtaata ggcgtaattt caatgtttgc agcaaaaagt cgaggtaatg ataatgtttt
     1501 gattacgggg aatggaagta aaaacagaat tggcaggaaa atcttggagg aagtttcaag
     1561 gatgtatggt atcaatttta tttttcctgc tgaagctgaa tttgcaacgg ctattggggc
     1621 agcattgtca caatcttaag atatttattt gggcaacatg ctgaaaattt agtatattgc
     1681 attgttctca atattgagat atgattatct aacaagtagt tgttatgtca caggaaaagg
     1741 aacatagaga gaaactaact aagaaggagc caaaaatgac tcttaaagag aagcgcgctg
     1801 caaaagaggc aaaacgcaaa gaaaaaagca atgaatagtc tgatgcttta aagattgaga
     1861 gaaggagctg actaaaaagt acttcttttt agtcagctcc tttttacttg tctttactcg
     1921 tcaacattta atatttctcc caaaaaataa tcaatgcaca ttgcaggata ttcctgtccc
     1981 ttttctactt tgtagatttg atcttcattt tcgtccatca gacataaaac ttcggtatcg
     2041 ttaactttaa aacacaattt aggacactct ttcccatcag cagatttgga aggtttgata
     2101 attatatagt catatttaat aggctgtata taaatatctt gtgttccatt tggaattgct
     2161 ttccaatctg tgtacttagt gtgtgaaatt aaatcttcaa aaccaatata cttgtgggag
     2221 tttgaaggtt tgatgtcgcg ggttacaatt ctttttttcc ctgaagcaat ctcttcgaat
     2281 tcagtctgct tgatgctaag agtgagcgtt ttcataattt gtatttatta taaaattaaa
     2341 tcaagatgtt gagattaaat ataatgaaaa gctcaacatc ttccaaattt tataaagatt
     2401 aattattcgg acagagtaaa gaagcgagta gcagcatgac gtctttctcc agtgtctcta
     2461 tcaataatat aataatcaat tttccatttg ccaatcaatt ttgtgcttgg attgattatt
     2521 aatatgctga aacctggttc aaagaagcca aaagggccaa atttctcttt gcttttatca
     2581 ggatatgtta tgacgtattc acaaagcagc gcgggttgcc ccttattatt aaggaaatct
     2641 ttaattggtg gaccaacaat ccatgttgca aatgtttttt ctgtgtcagt taacagtttg
     2701 aaattgtaaa tatcattttt tgaccagtag ttgctccttt gaacaatctt cagctctttg
     2761 ctatattcag acatgtcaag aataccaatt c
//
LOCUS       DAOTQL010000181         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_28143_length_2777_cov_5.038942, whole genome shotgun sequence.
ACCESSION   DAOTQL010000181 DAOTQL010000000
VERSION     DAOTQL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28143_length_2777_cov_5.038942"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1293)
                     /locus_tag="P5293_08270"
     CDS             complement(91..1293)
                     /locus_tag="P5293_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRR49927.1"
                     /translation="MKKTLIIALILSIASSLSAQKTKNSSADSAQKIKVGAESIEEYL
                     PMVQGKRVAVLSNQTGVIGNTHLVDTLIALKVNVVFIMSPEHGFRGDADAGEHVSSSV
                     DKKTGIPIKSLYEGNTGKPSSELLNQIDVIIFDLQDVGVRFYTYLTTMARMMEACAEN
                     GKKMIVLDRPNPLGFYVDGPILDMKFKSGVGWLPIPVVHGMTFGELAGMINGEKWLNN
                     GIQCDVSVIKCKNYTHASMYTLPVKPSPNLPEMRSIYLYPSMCYFEATPVSLGRGTDH
                     PFQMYGHPNMKGYAFNFTPRSISGAKNPPQLNRVCYGVDLTKEPSIEEINKKGINLEY
                     LIDAYHNLNLDNFFFNSFFELLIGRDYVRKMIIAGNSAAEIKSMWAGDVAKFKEQRKP
                     YLLYPDNN"
     gene            complement(1380..2726)
                     /locus_tag="P5293_08275"
     CDS             complement(1380..2726)
                     /locus_tag="P5293_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRR49928.1"
                     /translation="MKTKKIALILLLNLLSLATFADGYQINSQSARQIGMGHVGTAIK
                     LGSESILFNPAGLSYINGTFDASLGVTAIMSKVKYTNTAGTYKAESDNPVGTPIFGYI
                     GYRISPRFVAGIGLTNPGGNNLEWPDNWAGSHIIQNISLASFSLQPTLSYKISENLSV
                     GAGLMINWGNFELSKGILPVGALNNFINLVPSQYQAIITGASNISPMQITLKGDAKTA
                     YGYNIGLMFSPTNKLCLGISYRSKVMMKVEEGSAKITYASTELNQLIDLLAQASASLA
                     GAKVLDGKKVVAELPIPSNLNIGASYKFSDKLLVSGELQYVGWKAYDTLKIEFPELGA
                     AGKQHIPKNFSNSMIYRIGGEYYFSDSFTLRLGFIYDSTPVDKSYYGPETPATNKISI
                     TTGFTYKPAKSLAIDFGFQYLNGLKINGKYPTPLGSFDGEYDTRAFLPSLGLHFSF"
BASE COUNT          826 a          506 c          537 g          908 t
ORIGIN      
        1 aaagattgaa aaacttctcc ctgacgacac tttgagagta cagataaatg tactggaaaa
       61 tgaagtaagg gagattgtat tttaattggg ttagttattg tcagggtaaa gtaaatacgg
      121 tttacgctgt tctttaaatt ttgcaacatc tccagcccac atggacttga tttctgcagc
      181 agaatttcct gcaattatca tttttcttac ataatctctt ccaataagta attcaaagaa
      241 agagttgaag aaaaagttat caagattgag attgtggtag gcatctatca aatattccag
      301 gttaatcccc tttttgttta tttcttcaat ggaaggttct tttgtcaaat caactccata
      361 acagactcta ttgagttgag gcggattttt tgctccggag atgcttctcg gagtaaaatt
      421 aaatgcgtaa cctttcatat ttggatgacc atacatctgg aatgggtgat ctgttcctct
      481 tcctaggctg acaggggtag cctcaaaata acacattgaa ggatatagat atatagatct
      541 catttcagga aggtttggag atggtttaac aggaagagta tacatggagg cgtgagtgta
      601 gttcttgcac tttataacag aaacatcaca ctggatacca ttatttaacc atttttcacc
      661 attaatcatt cctgcaagtt caccgaatgt cataccatgt actacaggaa tgggtaacca
      721 tcctacgcct gatttaaatt tcatgtcgag aataggcccg tctacataaa agccaagtgg
      781 attggggcgg tcaagaacaa tcattttttt cccgttttct gcacaggctt ccatcattct
      841 tgccatggta gtgagatagg tataaaatcg aactccaaca tcttgaagat caaaaattat
      901 gacatctatt tgattgagaa gttcagagga tggtttacca gtattcccct catataggga
      961 tttaataggg atcccggttt tcttatcaac tgaactcgaa acatgttcgc ctgcatctgc
     1021 atctcctcgg aagccatgtt caggtgacat aataaatact acgttaactt taagtgcaat
     1081 caatgtgtca accaaatgag tgtttccaat aacacctgtt tggtttgaaa gaacggctac
     1141 acgtttgcct tgaaccatag gaagatattc ttctatagat tctgctccta ccttaatttt
     1201 ttgagcagaa tcagcagaac tgttttttgt tttttgcgct gataatgatg aggcaatact
     1261 taaaatcaga gcaataatta aagtcttttt cataactccg tcttttttta agaggttggt
     1321 tttattatga caaatataaa attattagca ttaattcgtc attagaatac aatatttatt
     1381 taaaagctga aatgtaaacc gagacttggt agaaaagccc tggtatcata ctctccgtca
     1441 aaactcccta acggtgtagg atacttgcca ttaattttga gtccgttgag atattggaag
     1501 ccaaaatcta tagcaagaga ttttgcaggt ttgtaagtaa aaccggttgt aatactgatc
     1561 ttgttggttg caggtgtttc cggtccataa taagacttgt ccacaggtgt tgaatcataa
     1621 atgaaaccaa gcctgagagt aaaactgtca ctgaaatagt attctcctcc gattctgtag
     1681 atcattgagt tgctgaagtt tttcggtata tgttgtttgc ctgcggctcc aagttccggg
     1741 aattcgattt taagagtatc ataagctttc catccaacat attgtaattc tccggaaacc
     1801 aaaagtttat ctgaaaattt gtaggatgcg cctatattta aatttgaggg tattggcagt
     1861 tcggccacga cttttttccc atcaagaacc ttggctccgg caagtgaagc actggcttgt
     1921 gccagcaagt caataagttg atttagttct gttgaagcat aagtaatttt ggcagaacct
     1981 tcttcaactt tcatcataac tttggatctg tatgaaatac cgaggcacaa tttattggta
     2041 ggactgaaca tcaacccaat attgtatcca taggcagtct ttgcatctcc cttaagtgta
     2101 atctgcatgg gtgagatatt tgatgcgccg gtaataattg cctggtattg agatggaaca
     2161 aggttaataa aattattcaa cgctcctaca ggcaatatgc ctttacttaa ttcgaaattt
     2221 ccccagttaa tcattaatcc ggctccaact gacaggtttt cactaatttt atatgacaaa
     2281 gtgggttgaa gggagaatga tgcaagagaa atattttgta taatatgaga tcctgcccaa
     2341 ttgtctggcc attcgagatt gttgcctccg ggatttgtca gtcctattcc tgcaacgaat
     2401 cttggagata ttctgtatcc tatgtatcca aatatcggag tacctactgg attatctgat
     2461 tcggctttgt atgtcccggc ggtattggta tatttaactt ttgacatgat tgccgtaact
     2521 cccagcgaag catcaaaggt tccattgatg tatgataatc ctgcaggatt gaacaggata
     2581 ctttcagaac caagtttaat ggctgttccc acatgtccca ttccaatttg tctggcactt
     2641 tgagaattga tttggtaccc atcagcgaac gtggcgagac ttaaaaggtt aagcagtaaa
     2701 atgagagcaa tcttctttgt tttcatcttg ttttgattat tattttcaaa acaaaactaa
     2761 tataatttac aaaaatc
//
LOCUS       DAOTQL010000182         2772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_28219_length_2772_cov_5.200589, whole genome shotgun sequence.
ACCESSION   DAOTQL010000182 DAOTQL010000000
VERSION     DAOTQL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28219_length_2772_cov_5.200589"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1273
                     /locus_tag="P5293_08280"
     CDS             113..1273
                     /locus_tag="P5293_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HRR49929.1"
                     /translation="MKRTFVVATVLLITIAVVLTSCSKDDSESGYNRIILVYLAANNN
                     LSNYASDNVSNLLKGYIPEENSNDILLVYSHLMDHTPVLLRIYRDDSGVPQQEVVANY
                     DNQNSTTPQVLNSVLKKIKIIFPAKEYGLILWSHGTGWLPQNYYDNPGMYGVFSPDPY
                     SDLVKSFGFESGVEMDIKEMAASIPYHFSFIIFDCCLMGGVEVAYEVKDKCDYILASP
                     AEVLATGFPYDVIMEPLFRSKADLEEVCQRYFDFYNSQRGVYKSATIALYRSDKFDEL
                     AAVCKSIFATNRDKLNSLQVTGIQGYFRLNKHWFYDIGDFISRIASQQEFMQFNDALS
                     KVVIKKLTTPTFLDFTINSYSGLSTYIQSNGSSFLDSYYKGYKWNIATEMVK"
     gene            1392..1973
                     /locus_tag="P5293_08285"
     CDS             1392..1973
                     /locus_tag="P5293_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRR49930.1"
                     /translation="MKTIKLNGAPRTVSNKQAIKTLRRNGMVPCVLYGPGVENQLFSV
                     NAKDLLLLTDTPNSYIVDLQIDGKNYKSILHAIQYHPVTDEPLHVDFLLVSDEKPVSI
                     NVPIIITGNSEGVRQGGKLAVSARKLRISAMLKDLPDSVTIDISTLGIGKNITAGDLK
                     YDNIQIITPKSTIICSVKMTRAALGTTAAPEAK"
     gene            1993..2557
                     /gene="pth"
                     /locus_tag="P5293_08290"
                     /pseudo
     CDS             1993..2557
                     /gene="pth"
                     /locus_tag="P5293_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            2554..>2772
                     /locus_tag="P5293_08295"
     CDS             2554..>2772
                     /locus_tag="P5293_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRR49931.1"
                     /translation="MSRIDKYLWSVRIYKTRTDATEACRSGRIRINEIQAKPSKEIKP
                     GDIISVRRGNIHFTYRVIADVERRQPAKM"
BASE COUNT          893 a          449 c          568 g          862 t
ORIGIN      
        1 agcccggaaa ctaaagcaat aagcataatt ttatatcttt tcataaatct aaatttgcac
       61 aaatatacct ctttgtgata tataatattt aaatttggtg aaaataagga agatgaaaag
      121 gacatttgtg gtggcaacgg tactgttaat tactattgca gttgtattga catcctgttc
      181 caaagatgac tctgaatcag gatacaatag gataattctt gtttatcttg cagctaacaa
      241 caatctcagt aattatgctt ctgacaatgt cagcaatctt ttaaaaggat atattccaga
      301 agaaaacagt aatgatattc ttcttgtgta ttctcatttg atggaccata ctcctgtttt
      361 gctgagaata tacagggatg atagcggagt gccgcagcag gaagttgtag ctaattacga
      421 taatcaaaac tcaaccacac cacaggtatt aaactcggta ctcaaaaaga taaaaattat
      481 ttttcctgct aaagaatatg ggcttattct ctggtcgcac ggaacaggct ggttgccaca
      541 aaactattat gacaacccgg gtatgtatgg agttttttct cctgatccct attcagattt
      601 agtgaaaagt ttcgggtttg aatccggagt tgagatggat attaaggaga tggctgcttc
      661 gataccctat cacttttcgt ttattatttt tgactgttgt ctgatgggag gtgttgaggt
      721 agcttacgaa gttaaagaca agtgtgacta tattttggcc tcaccggcag aagtgcttgc
      781 aaccggattt ccgtatgatg ttattatgga accactattt agatcaaaag cagatcttga
      841 agaggtgtgt caacgttatt tcgatttcta caattcacaa cgtggggttt acaagtctgc
      901 cactatagca ctttacagaa gtgataaatt tgatgaactt gctgctgttt gcaaaagtat
      961 ttttgctaca aacagggata aattaaattc tcttcaagta acgggtatac agggatattt
     1021 cagattgaac aaacactggt tttatgatat tggtgatttc atttcaagga tagcatcaca
     1081 acaagaattc atgcaattta atgatgcttt gagcaaagtc gttattaaaa agttgactac
     1141 accaacattc cttgatttta caattaacag ttattcagga ctcagtacat atattcagag
     1201 caatggctct tcgtttctgg attcatatta taaggggtac aaatggaaca ttgcaacaga
     1261 gatggtaaaa tgataaattg ttgtattgag ttatattttt tgtaactttg ccgcctctaa
     1321 aaaggaaact ttttggtgca atggggtccg gaccatttcc ggattgagta acacattttt
     1381 attaattcat tatgaaaaca attaaactta atggagctcc aagaacagta tccaacaaac
     1441 aggcaataaa aactttaaga agaaatggca tggttccgtg cgtactttat ggaccaggag
     1501 tagaaaacca gcttttttca gtaaatgcaa aggatttact gcttttgaca gatacaccta
     1561 attcttatat agttgatctc cagattgatg gcaaaaacta taaatccatt cttcatgcaa
     1621 ttcagtatca tcctgtaaca gatgagcctt tgcacgttga ctttctttta gtttcagatg
     1681 agaagcctgt gtcaataaat gttcctatta ttattacagg taattctgag ggagtgcgtc
     1741 agggcggcaa acttgcagtt tctgcaagaa aactcagaat atcagccatg ctcaaagatt
     1801 taccggattc ggttacaata gatatttcaa ctctcggcat aggaaagaat attacagctg
     1861 gagatcttaa atatgataac attcagatta ttactcccaa aagtactatt atctgttcag
     1921 tgaagatgac tcgtgcagca ttaggcacaa cagctgctcc tgaggcaaag taaatttctt
     1981 attcggtgag ccatgagctt tctgattgca gggttgggca atattggttc tgagtacgct
     2041 gatacccgtc ataatgcagg attttatgtg ttggatactc tggcaagatt atccaacata
     2101 gcttttataa gcagtcgtta tggatatata gccgaatata aatataaggg caaaaaattt
     2161 ttgctgctta aaccatcgac ttacatgaac cttagtggca aagctgtcaa ttactggtta
     2221 aatgcagaaa agattgatca ggagaatatg cttgtggtag tggatgatat agctttaccg
     2281 tttggctctt taaggatgcg gaagcagggt agtgacggag gtcataatgg tcttaaggat
     2341 ataatttctt ctttgggaaa cagtgatttt ccacgtctta gaataggtat tggaggagat
     2401 tttccgaaag ggagggcaaa tagattatgt acttggaaaa tggactgacc aggagataaa
     2461 atctctcccg gagattgcag ataaagctgt tgaagttatt aaattatttg gtacagaagg
     2521 cgctgaaaag gccatgaatt attgcaatgc aaaatgagta gaattgataa gtacttatgg
     2581 tcagtgagaa tatataaaac tcgtactgat gctactgaag cttgtcgatc aggtcgtata
     2641 agaataaatg aaattcaggc caaaccttct aaagaaatta aacctggaga cataatatct
     2701 gtccgaagag ggaacattca tttcacttac agagttattg cagatgtcga acgcagacag
     2761 cctgctaaaa tg
//
LOCUS       DAOTQL010000183         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_28341_length_2765_cov_3.880074, whole genome shotgun sequence.
ACCESSION   DAOTQL010000183 DAOTQL010000000
VERSION     DAOTQL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28341_length_2765_cov_3.880074"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /gene="dnaJ"
                     /locus_tag="P5293_08300"
     CDS             complement(<1..1163)
                     /gene="dnaJ"
                     /locus_tag="P5293_08300"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRR49932.1"
                     /translation="MAKRDYYEVLGVSKGASADEIKKAYRKLALKYHPDRNPGDKDAE
                     EKFKEAAEAYDVLSDPDKRSRYDQFGHAGMNGAAGSSGFSGGGFSMDDIFSQFGDIFG
                     GHFGSGFGGFSSFGSSGRSSSSKRSNRGSDIRIRVKLDLKEIAHGTEKTVKINKQVEC
                     PLCKGKGAVSEADIHTCETCRGTGQVSRVTQTFLGTMQTSSVCPSCHGEGKKIVRPCS
                     RCGGDGLVKDMEEISFKIPAGVAQGMQLTVQGKGNAAIHGGVNGDLLVVIEEEEHPEL
                     QRDGNDLIYTLFISVPDAILGCDAEVPSVDGKLRIKVDPGTQSGKILRLRSKGLPDVN
                     GYGYGDLLVFIQVWIPKRIDRQEREVLEKMRHSDNFKPSPTREDRNLFERLKK"
     gene            complement(1174..1752)
                     /locus_tag="P5293_08305"
     CDS             complement(1174..1752)
                     /locus_tag="P5293_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRR49933.1"
                     /translation="MKDKNKENCQENPDTVSQQVPVNEAADKVVEKDSVVPEPAAADV
                     KDEKEEKIAELNDRYLRLAAEFDNYRRRTAREKLELIQTASEDIIKGILPILDDCERA
                     IDLLKSIEGDHKAAIEGTELIYNKLYSYLESKGLKRMDVMGKELDTDFHEAVAQMPAP
                     DKKHKNKIINVAQQGYTLNGKVIRYAKVVIGV"
     gene            complement(1854..2534)
                     /locus_tag="P5293_08310"
     CDS             complement(1854..2534)
                     /locus_tag="P5293_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRR49934.1"
                     /translation="MENKCQFCNYRSMAESKLSEDELNELSHNSLNVSFKRGDVIIRQ
                     GTYSTNVAYLKSGLVKIHINGPYHEHVVKIVKSSNYLGLPTTFGDKINQYSVTAVENS
                     EVCFIDISFFRKMLAENHDFSSQIIVELCKGEIDSYQKCASRTQKQVRGKVADILIDF
                     ADSIYHSDSFTIQINQEDFGNLVDSSRESVSRVLSDFAKEEIIKILGKNVQILNKEML
                     YKISKLGW"
BASE COUNT          825 a          629 c          409 g          902 t
ORIGIN      
        1 attttcttca accgttcaaa aagattcctg tcttcccttg taggagaagg tttgaaattg
       61 tcagaatgcc gcattttctc aagaacttct ctttcttgtc tatcaatcct cttagggatc
      121 caaacctgaa taaatacaag tagatcgcca tagccataac cattaacatc cggcaaaccc
      181 ttagacctta atcgaagtat cttgcctgat tgagtacccg gatctacctt tattcttaat
      241 ttcccgtcga ctgatggcac ctctgcatca caaccaagaa tagcatcagg aacagaaata
      301 aaaagtgtat atatcaaatc gtttccgtcc ctctgcagtt caggatgctc ctcctcctca
      361 ataacaacga gaagatcacc gttcacacca ccatgtatgg cagcatttcc ctttccctga
      421 actgtaagtt gcattccttg agctacacca gcaggaattt taaaagaaat ctcctccata
      481 tccttcacga gaccatctcc tccacatctt gaacatggtc ttacaatctt tttaccttca
      541 ccatgacatg aaggacagac agaagatgtc tgcatggtgc cgagaaatgt ttgagtaact
      601 cttgaaacct gtcccgtacc cctgcatgtt tcgcaagtat gtatatcggc ttcagaaaca
      661 gcacctttac ccttgcataa tggacattct acttgcttgt ttattttgac tgttttttca
      721 gtaccatgag caatttcttt aagatctagc tttaccctga tccttatgtc ggagcctcta
      781 ttggatcgct tggaactact acttctacca gaacttccga aactgctaaa gcccccgaaa
      841 ccactaccaa aatgtccacc aaatatatca ccaaactgac taaaaatgtc atccattgaa
      901 aagccaccac cgctaaaacc actgcttcct gcagccccat tcatgcctgc atgaccaaat
      961 tgatcataac gcgacctttt atcaggatcg ctaagaacat cataggcctc agctgcttct
     1021 ttaaattttt cctcggcatc tttatctcct gggtttctgt ctggatgata tttaagagca
     1081 agctttctgt atgccttctt aatctcatct gcagatgccc ctttggaaac acccaaaacc
     1141 tcgtaataat ctctctttgc catcttaatt cagttaaaca ccaattacca ctttggcata
     1201 tctgataact ttgccattca aagtatagcc ctgttgggca acattaataa tcttattttt
     1261 atgtttttta tcaggagcag gcatttgtgc tactgcctca tgaaagtcgg tatccagttc
     1321 ttttcccatc acgtccatcc gtttaagccc tttggattcc aaatatgagt acagtttatt
     1381 gtagatgagt tcagtgcctt caatggcagc cttatggtcg ccctcaatgc ttttaagcaa
     1441 atcgattgca cgctcacagt catcaaggat aggaagaatg cctttaatga tatcctctga
     1501 agcggtttga attagttcca gcttctctct tgctgttctg cgtctgtaat tgtcaaattc
     1561 agcagcaagt ctcaaatatc tgtcatttaa ttctgcaatt ttttcttcct tctcatcctt
     1621 gacatctgct gcggctggtt caggtacaac tgaatctttc tctacaactt tatctgcagc
     1681 ctcattcacg ggaacttgct gagaaacagt atctggattt tcttgacaat tttccttatt
     1741 tttatctttc attaccataa taactttact ttccaacgcg aagatagtca caaaattttt
     1801 gccaaaggag tgttatacga caaaatgaca tttttagaca acttccacta acactaccag
     1861 ccgagtttgc taattttgta tagcatctct ttgtttagta tctgaacatt ctttcccaaa
     1921 attttaataa tctcctcttt cgcaaaatca cttaaaaccc tactgacact ttctcttgaa
     1981 gaatcaacca gatttccaaa atcctcctga ttaatctgta tggtaaaact atctgaatga
     2041 tatatactgt ctgcaaaatc tatcaaaata tcggccactt tgcctctaac ctgtttttgt
     2101 gttcggcttg cacatttttg gtaagaatct atctcaccct tgcacaactc cactattatc
     2161 tgactgctaa aatcatgatt ttctgctaac atttttctaa aaaaagatat atcaataaag
     2221 catacttctg aattttcgac agctgtcacc gaatactggt tgatcttatc tccaaaagtg
     2281 gtcggtaaac caagataatt ggaagattta acaattttta ctacatgttc gtgatatgga
     2341 ccattaatat gtatttttac taatccactt ttaaggtaag caacatttgt ggaatatgta
     2401 ccttgcctga taatcacgtc tccccttttg aagctaacat taagagagtt atgtgacaac
     2461 tcgtttaatt catcttctga aagcttactc tcagccatag atctataatt acaaaactga
     2521 catttatttt ccataagaaa attatttata ttgcaaatat aagaaattgt gatatacatc
     2581 actattttat aggatgagaa tcatgtcctt tggaaaaatt atcttgagta aaagatttta
     2641 ttttgtaaaa aaataaagat tatggaagaa aataatcaag tcacaattga actgttacaa
     2701 cagcaaattg acaaattgca aaaaaaggtt caaaaaatgg aaaatggaag aaaagaccaa
     2761 ctttc
//
LOCUS       DAOTQL010000184         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_28436_length_2759_cov_9.013683, whole genome shotgun sequence.
ACCESSION   DAOTQL010000184 DAOTQL010000000
VERSION     DAOTQL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28436_length_2759_cov_9.013683"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="P5293_08315"
     CDS             complement(<1..487)
                     /locus_tag="P5293_08315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRR49935.1"
                     /translation="MPNKKRNRRSLKWIGLLITVIIIIMVLLSMLPTPLKEPKADNVI
                     YPKNRPFISINHLLTNDKSELPETKKLDSYIKQFMRQWRIKGASVAIMKDEKLIYAKG
                     YGWADEEDSVRTEVKHIFRIASVSKLLTATAIMKLVENKMLALDQKPFAKGGILDISA
                     FQ"
     gene            complement(518..1219)
                     /locus_tag="P5293_08320"
     CDS             complement(518..1219)
                     /locus_tag="P5293_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRR49936.1"
                     /translation="MKKICTVTTVLLVITLSLSAQEKNFIDQNYIEITASAEKEVSPN
                     EIYISITIDEKDNKNNSLEKQERDMFSRLKALGIDLEKDMQVKDMETQLQEYFLKKNS
                     VVTSKSYQLKISSTDLLFKVFKELEKMSIPNVNIIRTDVSDKESIKNDVMIMAVNKAK
                     QRAELLANSVGRKLGKAIYIQHSEGYMRGNVVRNLAAGTKMMDINEEEIYSGPNLSFS
                     KISFNQTVYVKFSLE"
     gene            complement(1505..1726)
                     /locus_tag="P5293_08325"
     CDS             complement(1505..1726)
                     /locus_tag="P5293_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49937.1"
                     /translation="MAARRGKKRALIAVGHSILKSVYHVLSGVEEYKELGADYVNQRV
                     EKRRRLYLKRELEKLGYDVQLTQQERQAG"
     gene            complement(1801..>2759)
                     /locus_tag="P5293_08330"
     CDS             complement(1801..>2759)
                     /locus_tag="P5293_08330"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRR49938.1"
                     /translation="EMEEVSFRQVVSRGCGIDVHQKVAVATIGGEALRTQTREFGTTT
                     SSLTEMKEWLLGNGITHVAMESTGVYWKPVYNILEPSGIKVWIVNARHIKYVPGHKTD
                     KKDSAWICKLLLAGLLKPSYVPPKAQRNLRDLTRYRNKLVQNVASEKNRIIRILEDCN
                     IKLSSVMSSTSGVTATKLIDKLCDGKVVTREDIDEVYHGKLQCSKDDLYEACQGFITD
                     HHIFLLQTINRDIESTESLISELDSRIKESLSEYDNAMEVLRGIPGLNRKTVEDLIAE
                     IGLDMSVFPTEKHLASWAGMCPGNNESAGKKKAEESPTGTNR"
BASE COUNT          700 a          581 c          461 g         1017 t
ORIGIN      
        1 tttggaatgc agatatatcc aatattcctc ctttggcgaa tggcttttga tcgagggcaa
       61 gcattttatt ttcgacaagt ttcataatag cagtagccgt caatagtttt gacacagatg
      121 ctattctaaa aatatgctta acttctgttc tgaccgaatc ctcttcatct gcccatccat
      181 aacctttggc ataaataagc ttttcatctt tcattattgc cacagaggct ccttttatcc
      241 tccattgacg catgaattgc ttaatgtaac tgtccagttt tttagtttca ggcagttcgg
      301 atttgtcatt tgtaagaagg tgatttatag aaataaaagg tctatttttt ggatatatta
      361 cattgtctgc ttttggttct tttaatggag ttggtagcat tgaaagcaga accatgataa
      421 ttatgataac ggttatcaat aatcctatcc attttaaaga tcttctattt ctttttttgt
      481 ttggcattaa tgataaggaa agggataatt taagttttta ttccagtgag aatttaacat
      541 atacagtctg attaaagcta attttgctaa acgagaggtt gggcccagag tatatttctt
      601 cttcattgat atccatcatt tttgttccgg ctgccaaatt tctaacgaca ttgcctctca
      661 tatatccttc actgtgttgg atgtatattg cttttccaag ttttcttccc actgagtttg
      721 ccagcaattc agctctctgt ttcgctttat ttactgccat gatcatgaca tcgtttttga
      781 ttgattcctt atctgataca tctgttctga taatattaac gtttggaatg gacatctttt
      841 caagttcttt gaatactttg aatagaaggt cagtgctact tatttttagt tgataggatt
      901 tagaggtaac tactgaattt tttttcaaaa aatattcctg taactgagtt tccatgtctt
      961 ttacctgcat gtctttttca aggtcaattc ctagtgcctt taaccttgaa aacatatcac
     1021 gctcctgttt ctcaagagag ttgtttttat tgtctttttc gtcgattgta atactgatgt
     1081 aaatctcatt gggagaaacc tctttttctg cacttgctgt gatctcaata taattttgat
     1141 caataaaatt tttctcctgg gcagaaagag aaagagttat aacaagcaat acagtcgtta
     1201 ctgtacatat ttttttcata cgtaaatatt ttataattta ttacccataa acagggtatt
     1261 tttcagtgtt ttaaactaat attaataatt ctgacaaaac aaaagagagc acttatcgaa
     1321 taataaatgc gtggttattt tttcggcatt ggtatataaa cagctaaaaa tattaaaatt
     1381 tcatacacgt gctctttgtc actgtctttg ccgcttagct gttgagatca gtttcatgat
     1441 cggtttcctt actattcgta aatagcgggt accagtaaaa atcgaccttt gatttattgt
     1501 ttttttatcc tgcttgtcgt tcctgttgtg ttagctggac atcatatcca agcttttcca
     1561 actctctttt gagatatagc cttcgtctct tctcaacccg ttggtttaca tagtctgctc
     1621 caagttcctt atactcctct actccgctca acacatgata gacagacttc aggatcgaat
     1681 gtcctactgc tatcaaggct ctctttttgc ctcttcttgc tgccaatctg tgatatcttg
     1741 cagagtaaaa tgtattttta gtccttgttg ctgcccatgc tgcctcagtt attactgctt
     1801 ttacctgttt gttcccgtgg gtgactcctc cgcttttttt ttacctgcac tctcgttgtt
     1861 cccgggacac atacccgccc atgaggctaa gtgtttctct gtggggaaaa ccgacatatc
     1921 caggcctatc tccgctatca gatcttcaac ggtctttcta tttaatccgg ggattcctct
     1981 taaaacttcc atcgcattat catattcaga caagctctct ttgattcttg aatcaagctc
     2041 tgatatcaaa gattccgtag actcgatatc ccgattgata gtctgaagta aaaatatatg
     2101 atggtcggtt ataaatccct gacatgcttc atataaatca tccttgctgc attggagctt
     2161 accgtgataa acttcatcta tatcttctct ggtcacaacc ttgccatcac atagtttgtc
     2221 tatcaatttt gttgccgtga caccgcttgt agaactcatc acactggaca gttttatgtt
     2281 acaatcctcc agaatccgta ttatcctgtt cttctcagag gcaacattct gtaccagctt
     2341 attcctgtag cgagtcaaat ctcttagatt ccgctgtgct tttggaggaa cataacttgg
     2401 ctttaataat cctgccagca gtaacttgca aatccaggca ctgtctttct tgtctgtctt
     2461 atgtccggga acatatttga tatggcgagc attgacaatc cataccttga tgccactcgg
     2521 ttcaagaatg ttgtagacag gcttccagta aacccctgtg ctctccatcg ctacatgcgt
     2581 tatgccattg cccaataacc actctttcat ttctgtcaaa gaacttgttg tagtaccaaa
     2641 ctcgcgggtc tgagtccgaa gtgcttctcc tccgattgta gccactgcta ctttctggtg
     2701 tacgtctata ccacagcctc gtgatacaac ttgacgaaag gatacttctt ccatttcct
//
LOCUS       DAOTQL010000185         2756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_28490_length_2756_cov_4.866346, whole genome shotgun sequence.
ACCESSION   DAOTQL010000185 DAOTQL010000000
VERSION     DAOTQL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28490_length_2756_cov_4.866346"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1122
                     /locus_tag="P5293_08335"
     CDS             139..1122
                     /locus_tag="P5293_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRR49939.1"
                     /translation="MNSFGRNLRISIFGESHGEKIGLILDGIPAGISLSSDDFLKDLA
                     RRSGGKSGTTPRIEKDIPEIVSGVYKGFTTGAPIGILFSNDNTRSSDYLNFADIPRPG
                     HADFVSLIKSGGYVDLRGGGHHSGRITLTLVAAGVVAKKILGKILVKADLINVGGVED
                     TEDWELLLQKTREEGDSLGGIIECTVTNMPVGLGEPFFDSLESLLSHIIFAIPGIRGI
                     EFGDGFAAASMKGSEHNDPIVDSKGKTSKNGAGGINGGISNGNDLLFRVAVKPTSSIS
                     KGQRSFNFTTGKIEEFKIEGRHDTCFALRVPVVVEAATAIVLADLILSSTL"
     gene            complement(1112..2308)
                     /locus_tag="P5293_08340"
     CDS             complement(1112..2308)
                     /locus_tag="P5293_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRR49940.1"
                     /translation="MKKLSFLIFSLIISIGAISQPLMRISPEKAGFDATRLMNADRII
                     NESISKGDIPGAVLAVVRDDKMVYIKAYGNKSVYPTVEKMETTTIFDLASVSKCLGTT
                     LSFMQLVEQGKVRLTDNVAMYIKGFQGYADSVTGKTIDIRIVDLMTHSSGLPAYANID
                     ELTAKYGAPSPEALMDYISTCKRDFKPTTKFQYSCLNFITLQNVLQNITGMKLCDYAK
                     QNVYDVLELKNTFYNPDEEYFKNIAPTEKQKDGTVLLGKVHDPLARIFNFGNSGNAGL
                     FSNAEDMAVMASAIMNGGEYNGKRILGKLTVETMISIPPGFEHLGRALGWDLYSSYAS
                     NNGNLFPYGKTYGHTGYTGTSIVIDSVDKVAVILLTNRVHPEDKGGVVRLRALVANAV
                     AGAIIK"
     gene            complement(2389..>2756)
                     /locus_tag="P5293_08345"
     CDS             complement(2389..>2756)
                     /locus_tag="P5293_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49941.1"
                     /translation="GRLGGAEMARRELYKEGRTPLHTFRADIDYAHAEALTKVGILGI
                     KVWICNGEVYGKRDLSPNVGISTTGQGSQQTGGRPERRERRGNRNDDRRGRGESRGNQ
                     KSRGNRDRGGNRSYSPSES"
BASE COUNT          744 a          537 c          598 g          877 t
ORIGIN      
        1 acagaatagc tatggcaatc attactgcag ggttgaaaat caaagaaaat gttttccttg
       61 atgatttaaa ctgtatttca aagtcttttc ctgacttcat gagtaatttt aaatagacaa
      121 tttcattttc attattttat gaatagtttt ggcagaaact tgaggatttc catttttgga
      181 gagtctcatg gcgagaaaat aggtttgata ttggatggga ttcctgctgg aatttcgctt
      241 tcttcagatg attttcttaa ggaccttgcg aggcggagtg gggggaagtc aggtactact
      301 cccagaatag agaaagatat tcctgaaatt gtcagtggcg tttataaagg ctttactaca
      361 ggtgcgccga ttggcatttt atttagcaat gataataccc gctcctctga ttacttaaat
      421 tttgctgaca ttcccaggcc gggtcacgct gattttgtct cattgataaa aagcggaggt
      481 tatgtggatt taagaggcgg aggacatcat tccgggagaa taactcttac tctggtagcg
      541 gcaggtgtgg ttgcaaagaa gatacttgga aagatattgg ttaaggctga tcttatcaat
      601 gttggaggcg tagaagacac agaagattgg gagttacttc ttcaaaagac aagagaagag
      661 ggagattctc ttggaggtat aatagaatgt acagttacta atatgcctgt cggtcttgga
      721 gagccttttt ttgactcact ggaatcactt ctttcacata taatatttgc gatccccggt
      781 attcgaggga tagagtttgg agatggtttt gctgctgctt ccatgaaagg gtctgaacac
      841 aacgatccaa tagtggattc aaaggggaaa acttcaaaga atggtgctgg agggattaat
      901 ggaggtatat ccaatggtaa tgatcttttg tttagggtgg cagtgaaacc gacttcgagt
      961 atttcaaagg gtcagcgatc attcaatttt acaactggga agattgaaga atttaaaata
     1021 gaagggcggc atgacacttg ctttgctcta agagtcccgg tagttgtcga agccgccact
     1081 gctattgtat tagctgatct tatattatct tctactttat aatagctcct gctactgcat
     1141 ttgctactaa tgctctaagt cttacaacac ctcccttatc ttcgggatga acacggtttg
     1201 tcaggagtat tactgcaacc ttatctacag agtctataac aatggatgtg cctgtgtaac
     1261 ctgtatggcc ataagtcttg ccataaggga aaagattacc attgtttgag gcataagaag
     1321 agtaaaggtc ccagccgagg gctcttccca agtgctcaaa tcctggaggg atggagatca
     1381 ttgtttctac tgttagcttt ccaagaattc tttttccatt atattctcct ccattcatta
     1441 ttgctgaagc cattacagcc atgtcctctg cattagagaa tagacctgca tttcccgaat
     1501 tcccgaaatt gaaaattctt gcaagtggat cgtgaacttt tccgagcaga actgtcccat
     1561 ctttttgttt ctctgtagga gctatatttt tgaaatattc ttcatccggg ttgtaaaaag
     1621 tatttttgag ttccagaaca tcatatacat tttgcttcgc atagtcacaa agcttcatcc
     1681 ctgtaatgtt ctgaagcaca ttctgaagtg taataaaatt aagacaactg tattggaatt
     1741 tggtagttgg cttgaaatct ctcttgcatg ttgagatata atccattaag gcttcagggc
     1801 tgggagcacc atattttgca gtaagttcgt ctatattagc atatgctggc agtccggatg
     1861 agtgagtcat gagatctact attcgtatat ctattgtttt gcccgtcact gagtcggcat
     1921 atccttgaaa gcccttgatg tacatagcaa cattgtctgt taaacgaact ttcccctgtt
     1981 cgacaagttg cataaatgaa agggtagttc ctaaacattt actcaccgag gcaaggtcaa
     2041 aaattgtagt tgtttccatt ttctcaacag tcggataaac tgacttgttg ccatatgctt
     2101 tgatatacac catcttgtcg tctcttacaa cggcaagtac tgcgccgggt atatcgccct
     2161 tactgataga ctcgttgatg atgcgatctg cattcatcaa tctggttgca tcgaaaccgg
     2221 ccttttccgg ggagatcctc ataaggggct gggaaattgc tcctattgag atgatcaggg
     2281 agaaaattaa aaaggataat tttttcattt tttgaaagtt taagtaaatg gaaaatgcag
     2341 gcagagctaa ttaagctgcc cgcatttgat attcaatatt attgagaatc agctttcgga
     2401 aggagaatat gagcggtttc caccacgatc acgatttccc cggcttttct gatttcctct
     2461 cgattcacca cgaccccttc tgtcatcatt acggtttcct ctgcgttcgc gacgctcagg
     2521 acggcctcct gtctgttgac ttccctgacc agtagttgat atgcctacat taggagacaa
     2581 gtctcttttt ccataaacct ctccattgca tatccaaact tttatgccaa gtattcctac
     2641 ttttgtaagg gcttcagcat gagcataatc aatgtcggca cggaatgtat gcagaggagt
     2701 tctgccctct ttgtaaagtt ctcttcttgc catctcggct ccaccaagac ggccgc
//
LOCUS       DAOTQL010000186         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_28698_length_2742_cov_3.457760, whole genome shotgun sequence.
ACCESSION   DAOTQL010000186 DAOTQL010000000
VERSION     DAOTQL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28698_length_2742_cov_3.457760"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="P5293_08350"
     CDS             complement(<1..501)
                     /locus_tag="P5293_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49942.1"
                     /translation="MSAHIIITICILLLIAYIFDLTSSKTRIPSVILLLFLGWGVKQI
                     TLLLGIRLPDLNPVLPALGSVGLVLIVLEGSLELDLQRSKVSVITKSLFGALLPMIIV
                     AFGVALLFHITGGFPFRTALLNSVPFSIISSAIAIPSVRTLDSSLKEFVVYESSFSDI
                     LGVLFFN"
     gene            complement(525..851)
                     /locus_tag="P5293_08355"
     CDS             complement(525..851)
                     /locus_tag="P5293_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRR49943.1"
                     /translation="MKWLILVIAGLCETGFAFCLGKAKESSGIITTYWYIGFIAFLTV
                     SMLLLVKAVQELPVGTAYAVWTGIGAVGTVIVGILFFKEPADFWRLFFLTTLIISIVG
                     LKFVSE"
     gene            complement(1054..2214)
                     /locus_tag="P5293_08360"
     CDS             complement(1054..2214)
                     /locus_tag="P5293_08360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRR49944.1"
                     /translation="MKHIIKSKLVIIIGLLQLMLMSATVKAQEKIITVSGSVQFNEPM
                     FKMSILRQEGNEQVTIAEFDIDKDNKFNYKLKIDKPGIYTLDCKKWESIQFWGEDEDI
                     SVDFRGRDTARIKIKNPPFHFINGGPKNEVINHLNFINYRNYQGMIAVSKITYGSQFL
                     NDSVKNATTKKLYDFLDEERRSRIRYVAEMYSDRTSVVAALKHLDSQKDSELIKSVSN
                     ILFSKYPDYPPLVEYFNDIKENELNAARMEIGAYAPQFKYPDVNGKFYGPSDFKGKVL
                     LIDFWASWCGPCRAEIPNLKKVYEKYDRKDVEFLSVSIDKGKEEWIKALSQEKMPWIQ
                     VLAPKSGSEVSKLYQFSGIPFLVVLDKEGRIVSKYVRGVEEISKAIDKALAK"
     gene            complement(2239..>2742)
                     /locus_tag="P5293_08365"
     CDS             complement(2239..>2742)
                     /locus_tag="P5293_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49945.1"
                     /translation="LYNLYPKDIVDRKLRSIWENGANQFIKGEGESAVIDRQGFKEYV
                     KRMKNEGVEDYQTIEEKANIYYAIQTNDWKRMVSLIDAKISKNGLANIPVSELLGWGM
                     QIDKKCKDAKVRKHAAGWFEKYIPIFEANEAMRKAEAEKKGVMLAMSMINYPEEMKKL
                     YKSLSEN"
BASE COUNT          805 a          566 c          456 g          915 t
ORIGIN      
        1 gttaaaaaac agaactccca atatatctga gaaactactt tcgtatacaa caaattcttt
       61 taaagaggaa tccaatgtgc gaacacttgg aatagcaatg gcactgctta ttatgctaaa
      121 gggaacggag ttaaggagag ctgttctaaa tgggaagcca cctgtgatat gaaacagcaa
      181 ggcaacgcca aatgcaacta ttatcattgg cagtagcgct ccaaacaagg attttgtgat
      241 aacagatact ttggagcgtt ggagatcaag ctcaagtgat ccttctaaaa ctattagtac
      301 gagcccaaca ctcccaagcg caggtagtac aggattcaga tctggaagtc ggattccgag
      361 taatagagta atttgtttca ctccccagcc aagaaaaagc agcaaaatga cagaaggaat
      421 ccgggtcttt gaagatgtaa ggtcaaagat atatgctata agcaaaagta tgcagatagt
      481 tatgataata tgagctgaca tattgtgcta ttaaattgtt gttcttattc cgagacgaat
      541 ttcaatccaa caatagatat tattaatgta gtaaggaaaa aaagtctcca aaagtcagca
      601 ggttccttga aaaatagaat tccgactata actgttccaa ctgcaccgat acctgtccag
      661 acagcataag cagtgccgac aggcaattcc tgaacggcct tgacaaggag caacatgctg
      721 acagtcaaaa atgctataaa ccctatatac caataggttg tgataattcc tgaggattct
      781 tttgccttgc caagacaaaa ggcaaaacct gtttcacaca atcctgcaat taccaatatt
      841 aaccacttca ttatataaat tctgctgcaa aagtagaaaa ataaaagaga ttccttgcaa
      901 tctgaaggtt ttcttgctga tattcagtaa taaagccttg atttttagtg actgcagaca
      961 catcttttga tggtcgcata ttatataaac aaagaggcca cctacttttt aggcagcctc
     1021 tcctgggtta atttttgatt tgcgtagcaa ttgttactta gcgagggctt tgtcaattgc
     1081 ttttgatatc tcctctacac ctctgacata tttagaaact atccttccct ctttgtcaag
     1141 aacaacaagg aacggaatgc cagaaaactg gtaaagtttt gaaacttctg agccggattt
     1201 tggtgcgagt acctgtatcc atggcatttt ctcctgagac aaggctttta tccattcttc
     1261 tttgcctttg tcaattgaaa cacttagaaa ctctacatct ttgcgatcat acttttcata
     1321 tactttcttc agattgggaa tttctgctcg acatggtcca caccaggacg cccagaaatc
     1381 tattaacaac acctttcctt tgaaatctga cggtccataa aattttccat tgacatccgg
     1441 atatttaaat tgtggagcat aagcgccaat ttccattctc gcggcattta gctcattctc
     1501 tttgatatca ttaaagtatt ctaccagagg tggatagtct gggtatttgg aaaacaaaat
     1561 attgctgaca ctcttaatca actcgctgtc tttttgagaa tcaagatgtt taagagcagc
     1621 gacaacactt gttctatcag aatacatctc tgcgacatat cttattctgc tccttctctc
     1681 ctcatccaaa aaatcgtaca gtttctttgt cgttgcattt ttaacactgt catttagaaa
     1741 ttgtgagccg taagttatct tggatactgc aatcattccc tggtaatttc ggtagttaat
     1801 aaaattgaga tggttaatta cctcattttt tggtcctcca tttataaaat ggaacggtgg
     1861 gtttttaatc tttattcttg cagtatctct tcctctgaaa tcgacactta tatcttcatc
     1921 ttctccccag aattgaatgc tttcccattt tttacagtca agagtataaa ttccaggctt
     1981 gtcaatttta agtttataat tgaatttgtt atccttgtca atatcaaact cggcaattgt
     2041 cacttgctcg ttgccctctt gccttaaaat agacattttg aacattggtt cattgaattg
     2101 cacactgcca ctgacggtta taattttctc ctgtgctttt acagtggcag acatgagcat
     2161 taattgtaaa agtccaataa taatgacaag ttttgattta ataatatgtt tcataatgaa
     2221 tgactcataa tttatggatt aattctccga cagtgacttg tatagcttct tcatctcttc
     2281 gggatagttt atcatactca ttgcaagcat aacacctttt ttttctgctt ctgcctttct
     2341 cattgcctcg ttggcttcga aaattggtat gtacttttca aaccaacctg cagcatgttt
     2401 tctgacttta gcatccttgc attttttatc tatctgcatt ccccagccga gcaattctga
     2461 aacaggtatg ttagctaatc catttttgct gattttggca tcaattaacg ataccattct
     2521 cttccagtca ttcgtctgta tggcataata aatatttgcc ttctcttcga tagtttgata
     2581 atcctccacc ccttcatttt tcattctttt tacatactct ttaaagcctt gcctgtcaat
     2641 aaccgcactc tctccttctc ctttaataaa ctggtttgca ccattttccc atatacttct
     2701 gagtttcctg tcaactatat cttttggata aaggttgtat aa
//
LOCUS       DAOTQL010000187         2718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_29052_length_2718_cov_4.014270, whole genome shotgun sequence.
ACCESSION   DAOTQL010000187 DAOTQL010000000
VERSION     DAOTQL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29052_length_2718_cov_4.014270"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="P5293_08370"
     CDS             <1..1214
                     /locus_tag="P5293_08370"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRR49946.1"
                     /translation="ISAGGMVTTLLEMCFANCSGGIKADLSIFNEKDPVRLLFAEIAG
                     VIVQIPEIYISKIEEIAEEHGIVIYEIGEVTDERSLQIKSHKLDISLEIDHYRELWYK
                     TSYLLDVDQVGEQLAYKRYTNYLSQPLRFKFPKDFTGKKSNYKLPDITDLSSRPTAAI
                     IREKGSNGDREMAWALHLAGFAVKDVHMTDLISGREDLKNIRFIVFVGGFSNADTLGS
                     AKGWAGAFLYNKKAKESIEEFYSRNDTLSLGICNGCQLMAELGFLTPEERNRSPKMLH
                     NDSRKFESGFVGVTVEPSPSIMFSSLIGSELGIWVAHGEGKFSFPEPISNYNVALRYS
                     YDEYPANPNGSPDRVAGICSNDGRHLAIMPHPERCIYPHNWAYYTRSRIEDEITPWIE
                     MFVNAKKWILK"
     gene            1222..2565
                     /locus_tag="P5293_08375"
     CDS             1222..2565
                     /locus_tag="P5293_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRR49947.1"
                     /translation="MKSQKLPKIFVLDTNVILHDYRAIYNFQENDIVIPIVVLEELDK
                     FKKGDGAINYNAREFLREMDKITDNQLFDKGIRIGPGKGLLKIELGHPFSEQMLQAFF
                     EDIPDHRILATTQWIKEQNPDRIVALVTKDVNLRLKAKALSIDAQDYLTDKIAEERVE
                     NIKKEVIYLDDVSKDIIDRLFYKEDGVPVKEFNLNKLPANQLFSIKNPNGNNNILARY
                     RKTSNSVIGIKRVRAYGIDPRNDEQTFALDALLNPEIKLVSLTGTAGTGKTLLALAAA
                     MAQEQYFDQILLSRPVIPLQNQEMGFLPGDIKEKMGPYMLPLFDNLAVIQKAFKPTSK
                     EAVRIEEMLKSEKLLISPLAYIRGRSLSDSFFIIDEAQNLTPHEVKTIITRAGEGTKL
                     VFTGDIYQIDSPYLDMHSNGLTHLGEKLYGQELFEHINLVKGERSALSELAGKLL"
BASE COUNT          928 a          522 c          542 g          726 t
ORIGIN      
        1 acatatctgc cggaggaatg gttacaactt tgcttgaaat gtgctttgca aactgctccg
       61 gcggtattaa ggcagacctt tcaattttta acgaaaaaga ccctgtcaga ttactatttg
      121 cagagattgc cggcgttata gttcagatac cggaaattta tatctcaaaa attgaggaaa
      181 ttgccgaaga acatggaatt gtgatttatg aaataggtga agttacagat gaaagaagcc
      241 ttcaaattaa atcccataaa ttggacatct ctctggaaat agaccattat cgggagctat
      301 ggtataaaac atcatacctt ctcgatgtcg atcaagttgg tgaacagctt gcttataaac
      361 gttacacgaa ttacttgtct caacctcttc gatttaaatt tcctaaggac tttacaggga
      421 aaaaatccaa ttacaaacta cctgatatta ctgacttgtc ctcgagacct actgctgcaa
      481 tcatcaggga aaaagggtcc aacggagata gagagatggc ctgggcgctg catttagctg
      541 gttttgccgt caaggatgta cacatgactg acctcataag tggacgagaa gatctcaaaa
      601 atatacgttt tatagtgttt gtcggtggat tttccaatgc agatactctc ggctctgcca
      661 aaggctgggc aggagcattc ctatacaaca aaaaggcaaa agaaagcatt gaagagtttt
      721 attcaagaaa tgacactctt tcactcggaa tttgcaatgg atgtcagcta atggcagaac
      781 ttggtttttt aacacctgaa gaaagaaaca ggtcacccaa aatgctgcat aatgacagtc
      841 gcaagtttga aagcggattt gtaggagtta ctgtagaacc ttctccatca atcatgtttt
      901 cttctcttat cggatctgaa cttggaatat gggttgccca cggagaagga aagttcagct
      961 ttcctgaacc aatctctaat tacaacgttg ctttaagata ctcctatgac gagtaccctg
     1021 caaaccctaa cggatctcct gatagagtag ccggcatttg cagtaacgat ggcagacatc
     1081 tggccataat gcctcatcct gaaagatgta tataccccca caattgggct tattacactc
     1141 gtagcaggat tgaagacgaa attactccat ggattgagat gttcgtaaat gcaaaaaaat
     1201 ggattttaaa ataataatta tatgaaatca caaaaactac caaaaatatt tgtacttgat
     1261 acaaatgtca tattgcatga ttacagagca atatataact ttcaggagaa cgacattgtc
     1321 attccgatcg ttgttcttga agaactcgat aaatttaaga agggagatgg agccataaac
     1381 tataatgcga gagaattcct tagagagatg gacaaaataa ctgataacca gctttttgat
     1441 aaaggaataa ggattggtcc tgggaaaggg cttcttaaaa tcgaacttgg acatccattc
     1501 agtgaacaga tgttacaggc atttttcgaa gatattccgg atcatagaat attggcaacc
     1561 actcaatgga tcaaagagca gaatccagac agaatagttg ctcttgtcac caaggacgtc
     1621 aatctgagat tgaaagcaaa ggcactgtcc atagatgctc aggattacct aactgacaaa
     1681 attgcagagg agagagttga aaacataaag aaagaggtta tctatcttga tgacgtcagc
     1741 aaagacataa ttgacagatt gttttacaaa gaggatggag ttccagtcaa ggagtttaat
     1801 ttaaataaac ttccggccaa tcaacttttc tccattaaaa acccaaacgg caacaacaac
     1861 atactcgcaa gatacagaaa aacatccaac tctgtaatag ggattaaaag agtcagagca
     1921 tatggaattg acccaagaaa tgatgaacag acctttgccc ttgatgcact gcttaatcct
     1981 gaaatcaaac tggtttcact tacaggcact gccgggacag gcaaaacatt actcgcactt
     2041 gcagcagcaa tggctcagga acaatatttc gatcagatac tactctccag acctgtaatt
     2101 ccactgcaaa accaggaaat gggatttctt ccgggagata ttaaagaaaa aatgggtcct
     2161 tacatgcttc ccttattcga taaccttgcc gttatccaga aagcattcaa gcctaccagc
     2221 aaggaagcag tgaggataga agagatgctt aaaagtgaaa aacttctgat atctccgctg
     2281 gcatatatca gagggagaag tctatccgat tcgtttttta tcatagatga agcacaaaat
     2341 ttaactcctc atgaagtcaa gacaataata actcgtgccg gtgaaggaac taagcttgtg
     2401 tttacaggag atatctatca aatcgattca ccttacctcg acatgcattc caacggactg
     2461 acacatttgg gagaaaaact atacgggcaa gagttattcg aacacattaa tctagtcaaa
     2521 ggtgaaagaa gtgctctttc ggaattggcc ggaaaattgt tgtaaaaatt attatatttg
     2581 cccccctcat ttaaaaaaag aaaccaatta tttttaacac ataacctaat tttatcacaa
     2641 tgtctgatat taaaagagtt taccttttcg gcggtaaaac agccgaaggt gacggttcaa
     2701 tgaaaaatct cctgggag
//
LOCUS       DAOTQL010000188         2693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_29450_length_2693_cov_4.297953, whole genome shotgun sequence.
ACCESSION   DAOTQL010000188 DAOTQL010000000
VERSION     DAOTQL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29450_length_2693_cov_4.297953"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="P5293_08380"
     CDS             complement(<1..573)
                     /locus_tag="P5293_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR49948.1"
                     /translation="MVSINNLTVSFGGFTLLNDINFHISEKDCIGLVGKNGAGKSTIL
                     KIIAGIQRPTKGQILKPSGLSIGYLPQQMEHEKDKTVFEEALTAFSTLFDLKKKMDEI
                     SILLAERTDYQSKDYHKLIVELNDLNDRINFLSQENPEGMTEKTLLGLGFKRDEFCRQ
                     TSTFSQGWNMRIELAKILLKKPDLLLLDEPT"
     gene            complement(557..1306)
                     /gene="tatC"
                     /locus_tag="P5293_08385"
     CDS             complement(557..1306)
                     /gene="tatC"
                     /locus_tag="P5293_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRR49949.1"
                     /translation="MADEMTFWEHLDELRRVLFRSIIVIMVLMVVVFVNKTFVFEKII
                     LAPSRPDFFLYRILPGFSGFHLDLINIELSAQFFTHLSVSMALSIILAVPYILYQLWN
                     FVRPALYENEKRVVKKAFGFASILFFIGVVVGYMFVFPLTVRFLGSYQVSSSIQNTIS
                     LKSYISMFTQLILIMGIVFEMPALALILAKLGIISASFLKKYRRHALIILLIVAAIIT
                     PSGDAFTLFIVAGPLYLLYEFSILICRGINK"
     gene            complement(1306..1506)
                     /gene="tatA"
                     /locus_tag="P5293_08390"
     CDS             complement(1306..1506)
                     /gene="tatA"
                     /locus_tag="P5293_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRR49950.1"
                     /translation="MKTLLLFGTIGAPEIIIIALIVLLLFGGKKIPELMKGIGKGVKS
                     FKDGMKGIEDDIKDVNNEEEKK"
     gene            complement(1519..1932)
                     /locus_tag="P5293_08395"
     CDS             complement(1519..1932)
                     /locus_tag="P5293_08395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRR49951.1"
                     /translation="MFVHETELDVRYYETDMMGIVHHSNYIRYFECGRNDALISVGLP
                     IWKIEEMGVMTPVIRVECNYKHSAKNGERLKIVTMIKEPPMAKIIIYNEIFNAQGQLL
                     CSGSVTLGFINSVTRKAIRAPHFFLDKFSKYFDKQ"
     gene            complement(1934..>2693)
                     /locus_tag="P5293_08400"
     CDS             complement(1934..>2693)
                     /locus_tag="P5293_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRR49952.1"
                     /translation="DIINDHFDKFRISDALMQLYKLFWDDFCSWYLELVKPQYGEGID
                     VCTYNATIEFFDKLLRLLHPFMPFITEELWQNITKRRDGESIMIVDQPKAKSYNTAFL
                     KQFESVKECVVNVRNIRQSKRISPKESLSLFYRGDFEKEMFGVVGKMANIGTIESMNS
                     KIVDSLGVSFLVDKTEFFIPLEEKIDLNEELNKIEAELQYYNGFIENVMKKLSNEKFV
                     SCAPKNVVDMELKKKQDAEDKIRKLSELKRSLTR"
BASE COUNT          889 a          518 c          395 g          891 t
ORIGIN      
        1 agtcggctca tccagaagca gcagatcggg ttttttaaga agaatctttg ctaattcaat
       61 gcgcatattc catccttgac taaaagtgct ggtttgcctg caaaattcat ctctcttaaa
      121 accaagacct aaaagtgttt tctcagtcat gccctcagga ttttcttgac tgagaaaatt
      181 tatgcgatcg tttaaatcat taagttcaac tatcaattta tgataatctt tactttgata
      241 gtcggttctt tcagcaagca gaatgctaat ttcatccatt ttctttttaa gatcaaacaa
      301 tgtcgaaaat gcagttaagg cttcttcaaa tacagttttg tctttttcgt gttccatttg
      361 ttgaggaagg tacccaatgg ataaacctga aggttttaga atctgacctt ttgttggtct
      421 ctgaatcccg gcaataattt ttaaaatagt agatttcccg gctccatttt tacctacaag
      481 acctatacaa tctttctcag aaatatgaaa atttatatca tttagcagag taaatccacc
      541 aaaagagaca gttagattat ttattgatac cacgacaaat caaaatacta aattcataaa
      601 gcaaatatag cggaccagct acaataaaaa gagtaaaagc atctccactt ggagtaataa
      661 ttgctgcgac aatcaaaagg ataattaatg catggcgcct gtattttttc aaaaatgagg
      721 ctgatataat tccgagtttt gcaagaatga gtgcaagtgc aggcatttca aaaacaattc
      781 ccataatcaa tattaattga gtaaacatcg atatgtagga tttaagggag atggtgtttt
      841 gaattgaaga actcacctgg tatgatccta aaaaacgaac tgtaagtgga aaaacaaaca
      901 tatagcctac aactactcca ataaaaaaaa gtattgaagc aaatccaaaa gcttttttga
      961 caactcgttt ttcattttcg tataaggccg gtctgacaaa attccatagc tgataaagaa
     1021 tatatggcac agccaatatt attgataaag ccatggaaac actcaaatgt gtaaaaaact
     1081 gagcagaaag ttcaatgtta ataaggtcaa ggtgaaatcc agaaaaaccc ggcagtattc
     1141 tgtacagaaa gaaatcagga cgagaaggtg caagtattat tttttcaaaa acaaaagttt
     1201 tattgacaaa aacaacaacc attagcacca ttatgacaat gatagacctg aacaagactc
     1261 ttctgagctc gtccagatgc tcccagaatg tcatctcatc tgccactatt ttttctcttc
     1321 ctcgttgtta acgtccttaa tatcgtcttc aatgcctttc attccatctt tgaagctttt
     1381 aactcccttt ccaatgcctt tcatcagctc gggaattttt ttccctccaa aaagtaacaa
     1441 aacaatcaga gcgattataa ttatttcagg agcaccaatg gttccaaata ataaaagagt
     1501 tttcattgtt tattgttttt attgtttgtc aaaatatttg gaaaatttat ccagaaagaa
     1561 atgtggagca cggattgcct ttcttgtcac gctgttaata aacccaagag ttacacttcc
     1621 actgcacaaa agttgacctt gagcattgaa tatctcattg taaattataa tttttgccat
     1681 cggaggttcc ttaatcatag tcacaatttt aagtctttct ccattttttg cagaatgttt
     1741 gtaattacat tcaaccctta taacgggagt cattacaccc atctcttcga ttttccaaat
     1801 tggaagtcca acggaaatta aagcatcatt tcttccacat tcaaaatacc gaatataatt
     1861 tgagtgatga acaataccca tcatatcggt ttcatagtac ctgacatcca attctgtttc
     1921 gtgtacaaac atatcacctt gttaatgatc ttttaagttc acttagcttc ctgattttgt
     1981 cttcggcatc ttgttttttc tttaactcca tgtctactac atttttaggc gcacaggaaa
     2041 caaatttttc gtttgagagt tttttcataa cattttctat aaaaccatta tagtattgta
     2101 attcagcttc aattttattt aactcttcat tcaaatctat tttctcttca agagggataa
     2161 aaaattcagt tttatctaca aggaatgaaa ctcccaaact gtcaacgatt ttactgttca
     2221 ttgactctat tgtgccaata tttgccatct tcccaaccac tccaaacatc tctttctcaa
     2281 aatctcccct gtaaaacaga gaaagagact cttttggaga gatcctcttt gattgtctga
     2341 tatttctcac attcacaaca cattctttaa cactttcaaa ttgtttcaaa aaggctgtat
     2401 tataactttt agcttttggc tgatcaacaa tcattatact ttcgccgtct cttcttttag
     2461 taatgttttg ccacaactct tctgtaatga aaggcataaa aggatgaaga agccttagca
     2521 atttatcaaa aaattcaata gtggcattat atgtacagac atcaattcct tctccatact
     2581 gaggctttac aagctctaaa taccatgaac aaaaatcatc ccagaacagt ttgtaaagct
     2641 gcataagagc atcggatatt ctgaatttat caaagtgatc attaattata tca
//
LOCUS       DAOTQL010000189         2645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_30159_length_2645_cov_5.169112, whole genome shotgun sequence.
ACCESSION   DAOTQL010000189 DAOTQL010000000
VERSION     DAOTQL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30159_length_2645_cov_5.169112"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="P5293_08405"
     CDS             complement(<1..523)
                     /locus_tag="P5293_08405"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRR49953.1"
                     /translation="MTNLGYRLIDPLEIEENLIKLISSDWMLVTAGNKAKFNTMTANW
                     GGAGYLWNRPVVFVFVRPERYTYQFIESSEKFTLSFFDEKYRSALDICGSKSGRDCNK
                     VAEASLTPVFTELGNTTFKEARLVFECYKLYSEMLSKNFFNDPGPLNAHYEGHSGLHK
                     LYIAEILKVWETEK"
     gene            complement(539..1132)
                     /locus_tag="P5293_08410"
     CDS             complement(539..1132)
                     /locus_tag="P5293_08410"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRR49954.1"
                     /translation="MKVDHHLLSYLYDDMIEAGCDEAGRGCLAGPVFAAAVILPHDFF
                     HPLLNDSKQMTRKNRELLRPIIEKEAISWAVASVSNIEIDRINILNASIFAMHKALDA
                     LDTRPEFIIVDGNKFRNYNNIPHICFIKGDGRFTSVAAASVLAKTHRDEYMVSIASDY
                     PQYGWEKNFAYPTAAHREAIMKYGPCEYHRTSFRLLK"
     gene            complement(1174..1572)
                     /locus_tag="P5293_08415"
     CDS             complement(1174..1572)
                     /locus_tag="P5293_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRR49955.1"
                     /translation="MNDSDIKKRIRELRESLGYTQEDMANYLKMSLNSYRQLESGKTS
                     IINKRVFSIAQLFNMSLSELIFGTNKETSTDEKIAEIAKSYQEQIEKIEADSRIKMIE
                     LEDENKILKMALESKESIIGILKESLNKYR"
     gene            1762..2070
                     /locus_tag="P5293_08420"
     CDS             1762..2070
                     /locus_tag="P5293_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49956.1"
                     /translation="MKTLYSNIALLEMEALFENRKIYLKSEITPSLIADLLKIKRRRL
                     EGIVNNSFGLSIDNIVNMYRLQYARQLLISGEKYDSLWEKSGFSSRVDMERAFENIVV
                     "
     gene            2140..>2645
                     /locus_tag="P5293_08425"
     CDS             2140..>2645
                     /locus_tag="P5293_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRR49957.1"
                     /translation="MKSYPTQSIRNVVLLGSPKSGKTTLAETMLFEGKVIDRRGSVEA
                     KTTVSDNTEIEQIYQRSIYPTLLYAEFMDHKLNIIDTPGSDDFIGGVISAFKVADTGV
                     MIVNAQQGVEVGTEILARYAEKYRMPLIIGVNQLEHEKANWENSIESLKLAFGKKIVL
                     VQFPVEQGA"
BASE COUNT          826 a          460 c          494 g          865 t
ORIGIN      
        1 tcttttctgt ctcccaaacc ttgagtattt ctgctatata tagtttatga agtccggagt
       61 gtccttcata atgtgcatta agagggccgg gatcgttaaa gaaatttttt gacagcatct
      121 ctgagtagag tttataacat tcaaagacaa gccttgcttc tttgaaggtg gtatttccca
      181 actcagtaaa cacgggagtt agtgatgctt cagcaacctt attgcaatct ctgccacttt
      241 ttgatccaca tatatcgaga gcagaacggt atttttcatc aaaaaaactt aatgtgaact
      301 tttctgaact ctcaataaat tgataagtat accgctcggg acgaacaaat acaaatacaa
      361 cgggccgatt ccaaagataa cctgcgccac cccagttggc agtcattgta ttaaattttg
      421 ctttattgcc tgcagttacc aacatccagt cggaggagat tagttttatt agattttcct
      481 caatttcaag aggatctatc agtctgtatc ccaagtttgt catttttgta aaatttagct
      541 atttcaacaa tctaaaagag gtccgatgat attcacacgg gccatatttc ataattgcct
      601 ccctgtgagc tgctgtaggg taggcaaaat tcttttccca tccatactgg gggtagtctg
      661 aagcaattga taccatatat tcatcgcggt gagttttggc aagtaccgaa gcagcagcta
      721 ctgaggtaaa gcgaccatct ccctttataa aacaaatatg gggtatatta ttataatttc
      781 gaaatttgtt gccatcaaca ataataaatt ccggtcttgt atcaagtgca tcaagagctt
      841 tgtgcattgc gaaaattgat gcgttcaata tattaatcct gtcaatctca atgttactga
      901 ccgatgctac agcccaggat attgcttctt tctcaataat tggacgcaat aactccctgt
      961 tctttcgtgt catttgtttg gaatcattga gcagagggtg aaagaaatca tgaggaagta
     1021 taactgcagc tgcaaagaca gggcctgcca gacatcctct gccagcctcg tcacaacctg
     1081 cttcaatcat gtcgtcatag agataggata ataaatgatg atccactttc atgagcttgg
     1141 aaatattccg aaatttagca aaaagagata tatttagcga tatttattta aactctcctt
     1201 tagaatacca attatgcttt ctttagattc gagtgccatc ttgagtattt tattttcatc
     1261 ctcaagctca atcattttta ttcgggaatc ggcttctatt ttctcaattt gctcttgata
     1321 agactttgct atttcagcaa ttttttcatc tgtcgaagtt tctttattag tcccaaagat
     1381 cagttcagaa agtgacatgt tgaataattg tgcaattgaa aagactcttt tattaataat
     1441 ggatgtttta ccagattcaa gttgtctgta agagtttaaa gacattttaa gataattagc
     1501 catgtcctct tgtgtatatc caagagattc tctcagttcc cgaatccttt tttttatgtc
     1561 actgtcattc ataataacaa atataaaata atgtcttatg tgacttggaa caacaaaata
     1621 ttgttattgt gacatgaaat agatgttatt ttaaaattta aaaatcaaaa ttgaacaaat
     1681 taaatcaata atcagacaaa tgacatataa agcaggttgg aaagggtaaa tattgtatta
     1741 ttttcgaaaa aaatcacaat tatgaaaaca ctttattcaa acatcgctct tttggaaatg
     1801 gaggcacttt ttgaaaacag aaagatctat cttaaaagtg agataacgcc ctctcttata
     1861 gctgatcttc ttaaaattaa aaggcggagg ttggagggaa ttgtcaataa ttcttttggt
     1921 ctttcgatag ataacattgt caatatgtac agacttcagt atgccaggca attgttaatc
     1981 tcaggagaaa aatatgactc tttatgggaa aagtcgggtt tcagttcgag agttgatatg
     2041 gagagagctt tcgaaaacat tgtcgtgtaa aaactttttt gtacttttga atctcttaag
     2101 tcaatgaatt tataattaat aaatattcag cgtattagta tgaaaagtta tcctacccaa
     2161 agcatccgga atgtggtgtt actcggtagt cccaaatcgg gtaaaaccac tctggcagaa
     2221 acaatgttgt ttgaaggtaa agttatcgat cgtaggggtt cagttgaagc aaaaaccaca
     2281 gtcagcgata ataccgaaat agaacagatt tatcaacgtt ccatttatcc aactcttctt
     2341 tatgcggagt ttatggacca taagcttaat attattgaca ctccgggctc tgacgatttt
     2401 ataggtggtg ttatttccgc atttaaagtt gctgacacag gagttatgat tgtgaatgca
     2461 cagcagggcg tagaagtagg tactgagatt ttggctcgat atgccgaaaa atacagaatg
     2521 cctttgatta ttggtgtcaa tcagcttgaa catgagaagg ctaattggga gaattccatc
     2581 gaaagtttga aattggcttt cggcaaaaaa attgtactgg ttcaatttcc tgttgagcag
     2641 ggagc
//
LOCUS       DAOTQL010000190         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_30279_length_2637_cov_4.219985, whole genome shotgun sequence.
ACCESSION   DAOTQL010000190 DAOTQL010000000
VERSION     DAOTQL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30279_length_2637_cov_4.219985"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="P5293_08430"
     CDS             complement(<1..757)
                     /locus_tag="P5293_08430"
                     /inference="COORDINATES: protein motif:HMM:NF027542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M64 N-terminal domain-containing
                     protein"
                     /protein_id="HRR49958.1"
                     /translation="MKKSYIFIFFALLTFSAKGQKQDNLVYYDYFTDQRLRIDLVFAG
                     NSEKQELFLKGLHKEKEWSGTKTNLISPFDYGEYHMQVAAGDGKIIYTTGFNNLFQEW
                     RTTAEAKSVSKAFSGACWIPWPKDVVKVIFYERKKSNGKLAKLCSFQIDPKDMSINCE
                     DENDFEIVSLINNGDKSKKVDLLFVAEGYRADQMDKFLQDAKKFTDYLFNIEPYKSRK
                     DDFNIWAIKSISKDSGPDIPHQGIWKQTVLESNF"
     gene            complement(769..2106)
                     /gene="purB"
                     /locus_tag="P5293_08435"
     CDS             complement(769..2106)
                     /gene="purB"
                     /locus_tag="P5293_08435"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRR49959.1"
                     /translation="MDNILAISPVDGRYYEQVEGLSEYFSEFALIKYRLYVEIEYFIF
                     LCEIPLPQLSEVKQETFGTLRNIYKDFSLDDAKEIKGIETVTNHDVKAVEYFVKKRFD
                     TFGLSKFKEFIHFGLTSQDINNTSIPLSMKDALKELYNPLLQSLISKLEELAEEWKDI
                     PMLARTHGQPASPTRLGKEIEVFAVRLKEQYNMYEMIPLAAKFGGATGNLNAHHVAYP
                     SIEWNKLANDFVSNRLGLKRSYPTTQIEHYDYFAALFDCLKRINTILIDLCRDMWLYI
                     SMEYFRQKIKEGEVGSSAMPHKVNPIDFENAEGNLGFANAIYEHLSSKLPVSRLQRDL
                     TDSTVLRNIGVPVAHSIISFKSVLKGLNKLILNRSAIEKDLNANWAVVAEAIQTILRR
                     ENYPNPYETLKALTRTNLPVNQTLISEFIDNLDISDEVKNELRAISPETYTGI"
     gene            complement(2118..>2637)
                     /locus_tag="P5293_08440"
     CDS             complement(2118..>2637)
                     /locus_tag="P5293_08440"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRR49960.1"
                     /translation="FIGDFIINDISAGEDDPEMLNMVAKLELPYIAMHKRGTPETMQS
                     HCQYVDVVSEVRNYFVNFIETATQKGIKEIIIDPGFGFAKDNPQNYTMLNRLDEFKIK
                     KSEDGSFWPILVGISRKSMIYKFLNITPEESLPVTVALNLQALLKGADILRVHDVKEG
                     KQIVKIFSNFMA"
BASE COUNT          774 a          484 c          463 g          916 t
ORIGIN      
        1 agaaatttga ttcgagaact gtttgcttcc aaatgccttg atgaggaata tcaggcccac
       61 tgtctttgga aattgacttg atagcccaaa tgttaaagtc atctttacga gacttgtaag
      121 gctctatgtt aaaaagataa tcagtaaatt tcttggcatc ttgaagaaac ttatccattt
      181 ggtcagctcg atatccttcg gcaacaaaga gaagatccac tttttttgat ttatcgccgt
      241 tatttatcag agatacaatc tcaaagtcgt tttcatcttc acagtttatc gacatatctt
      301 ttggatcaat ttgaaaactg cagagttttg caagcttgcc gtttgatttt tttcgttcat
      361 aaaaaataac cttgacgaca tcttttggcc aagggatcca gcaagcgcca gaaaaagcct
      421 tggatacaga tttggcttcg gcagttgtcc tccattcctg gaataaattg ttaaatccgg
      481 tagtataaat tattttaccg tcgcctgcag ccacttgcat gtgatactct ccataatcga
      541 aaggtgaaat aaggtttgtt ttagtaccac tccattcttt ttccttgtga agtcctttta
      601 aaaataattc ctgtttttcg gaatttcctg cgaacacaag atctattcta agcctttgat
      661 cggtaaaata atcgtaataa accaaattgt cctgtttttg tcctttggct gaaaaagtga
      721 gtaaagcaaa aaaaataaaa atatatgatt ttttcatttt atgagttgtt atatgccggt
      781 gtacgtttct ggtgaaatag cccgaagttc attttttact tcatcactta tatcgaggtt
      841 gtcgataaat tcggaaatga gtgtctgatt aacaggaagg tttgtcctgg taagagcttt
      901 taacgtttca tagggatttg gataattttc tcttctaagt attgtctgta tagcttcggc
      961 cacaacagcc caatttgcat tcagatcttt ttctattgca gacctgttta atataagctt
     1021 gttcaatcct tttaacactg atttgaagga tattatgctg tgggctaccg gaaccccgat
     1081 gttcctaaga acagtggagt cagtcagatc tctttgaagt ctcgaaacag gtaacttcga
     1141 ggaaagatgt tcatatattg catttgcaaa gcctaaattg ccttctgcat tttcaaagtc
     1201 tatagggttg actttgtgtg gcatggctga tgaaccaact tctccttcct taattttttg
     1261 cctgaaatat tccattgata tatatagcca catatcacga caaaggtcaa ttaaaatggt
     1321 atttattctt ttaagacaat caaaaagtgc cgcaaaatag tcgtaatgtt caatctgtgt
     1381 tgtgggataa gatcttttta atcccagcct gttactcaca aaatcgtttg caagtttgtt
     1441 ccattcaatt gaaggataag ctacatgatg agcatttaaa ttgcctgtgg cacctccaaa
     1501 tttggctgcc aacggaatca tttcatacat gttatattgt tccttaagac gaacggcaaa
     1561 aacctcaatc tctttgccaa gccttgtagg agaggctggt tggccgtgtg ttcttgcaag
     1621 catcggtata tctttccact cttctgcaag ttcttcaagc ttagatatga gtgattgcaa
     1681 caaagggtta tataactcct tgagtgcatc tttcatagag agtggaatgg aggtattatt
     1741 aatatcctga gaagtaagtc caaagtgaat aaattctttg aatttggaaa gaccaaatgt
     1801 gtcaaatctt ttttttacaa aatattcaac agctttcaca tcatgatttg taacagtttc
     1861 aatgccttta atctcttttg catcatcaag tgaaaaatcc ttgtaaatat tgcgaagtgt
     1921 gccaaaagtt tcttgtttga cttctgaaag ctgtggcaga ggaatttcac ataagaatat
     1981 aaaatattca atctctacat aaagtctgta ttttatcagt gcaaattctg agaagtactc
     2041 agataatcct tcaacctgtt cgtaatatct tccgtccacg ggtgagattg caagaatatt
     2101 gtccatattg tgtattatta agccataaaa ttactaaaga ttttcacaat ctgtttgccc
     2161 tctttaacat catgtactct taatatgtct gctcctttga ggagtgcttg tagattaaga
     2221 gcaacagtta caggcaatga ttcttcggga gttatgttta aaaatttgta aatcatgctt
     2281 tttctggaga ttcctactaa aataggccag aaacttccat cttcactttt ttttattttg
     2341 aactcatcca atctattcaa cattgtataa ttctgaggat tatccttagc aaatccgaat
     2401 cccggatcaa taattatctc ttttattccc ttttgtgtag ccgtttctat gaaattaacg
     2461 aaatagttgc gaacttcact taccacatcc acatattggc agtggctttg catagtttca
     2521 ggggttcccc ttttgtgcat ggcaatgtat ggtaattcaa gctttgcaac catgttaagc
     2581 atttcaggat catcttctcc ggcagaaata tcatttatga taaaatcacc aataaaa
//
LOCUS       DAOTQL010000191         2625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_30465_length_2625_cov_4.027626, whole genome shotgun sequence.
ACCESSION   DAOTQL010000191 DAOTQL010000000
VERSION     DAOTQL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30465_length_2625_cov_4.027626"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..687)
                     /locus_tag="P5293_08445"
     CDS             complement(10..687)
                     /locus_tag="P5293_08445"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRR49961.1"
                     /translation="MDKRHFAIIVAGGVGTRMGTDIPKQFINLGGKPLLLRTLEVFLN
                     LAFPVEIIIALPVAYMDRWKETCAAAGLDGTHKIVAGGITRFHSVKNALSIIPKGVLV
                     AVHDGVRPFVHSEFIESLYAKASECGSAIPVVKPADSMRVLMSDGKSKPVNREEFVFV
                     QTPQVFDSEILLNAYEQPYSQSFTDDASVVESAGCQISLVEGDPINIKITQQEDLLIA
                     KAILSVF"
     gene            759..2282
                     /gene="purH"
                     /locus_tag="P5293_08450"
     CDS             759..2282
                     /gene="purH"
                     /locus_tag="P5293_08450"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRR49962.1"
                     /translation="MDKKIIKKALISVYHKDGIDTIAWKLHGAGVEIISTGGTYDYLK
                     SLSIPVTSVEEITTYPSILGGRVKTLHPKIFGGILGRREIDSDLKNFDEYKIPQIDLV
                     IVDLYPFEQSVASGVSHEEIIEKIDIGGISLIRAAAKNYKDVVVIPCKGLYDKFLTIL
                     DERGAETSLEERHYFATEAFNISSHYDTAIFTYFNREEKIQVFKKSILTSRPLRYGEN
                     PHQSASFYGSEENLPEQLHGKEISYNNLLDVEAAIELISDFTDTTVAILKHNNACGCA
                     SRETLKDAWSAALACDPVSAYGGIIITNRIIDADTAKEMNTLFCEVVIAPEYEEEALN
                     ILKSKKNRIILVNRGDLRSDYKFRSLLEGVLVQERDTALETRESLKCVTAKAPSESEI
                     EDLIFANKIVKHSKSNAIVLAKDRTLLASGVGQTSRVDALKQAIDKAHSFGFSTEGAV
                     MASDAFFPFSDCVEIAHKAGIKSVIHPGGSIRDNDSIVYCDENNISMVMTGIRHFKH"
     gene            2301..>2625
                     /locus_tag="P5293_08455"
     CDS             2301..>2625
                     /locus_tag="P5293_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRR49963.1"
                     /translation="MAFSFLTQDLAIDLGTANTVIMINDKIVVDEPSIVAIDQNTGKP
                     IAFGQKARMMHGKTHANIKTIRPLRDGVIADFNACEMMIRGFVKMINYKHSLFTPSLR
                     MVVSIP"
BASE COUNT          851 a          522 c          502 g          750 t
ORIGIN      
        1 catctacact caaaaaacac taagaattgc ttttgcaatt aacaggtctt cctgctgagt
       61 tatttttata tttatgggat ctccctctac aagagaaatt tgacagcctg cactctcaac
      121 cactgaggcg tcgtctgtaa atgactgaga atatggttgt tcataagcat ttagcaaaat
      181 ttctgaatca aacacctgtg gagtttgtac aaaaacaaac tcttctctgt ttacaggctt
      241 acttttacca tcagacatca aaaccctcat tgaatctgct ggtttaacaa caggaatagc
      301 tgatccacat tctgaggctt tggcgtataa tgattctata aattcagaat gaacaaatgg
      361 ccttactcca tcgtgaacag caaccaatac tcctttggga ataatactta gagcattttt
      421 taccgaatga aagcgagtta taccgcccgc aacaatttta tgtgtcccat caagacctgc
      481 tgcagcacat gtctccttcc atctgtccat atatgcaacc ggcaatgcga taataatttc
      541 cacaggaaaa gcaaggttta aaaaaacttc aagagtcctt aaaagtaacg gtttcccccc
      601 aagatttata aattgcttgg ggatgtcagt tcccatcctt gttccaactc ctccggcaac
      661 aatgattgca aagtgtcttt tatccatttt tttaatttta tagagacaaa tttatatatt
      721 aattgccttt tttagtagtt ttgcaatcat catcacatat ggacaagaag ataataaaaa
      781 aagctctcat ctcggtatat cataaagatg ggattgacac tattgcctgg aaactacacg
      841 gggcaggtgt tgaaataatt tcaacaggag gtacttacga ttatttaaaa agtctttcca
      901 ttccggtgac ttcagtagaa gagatcacta catatccatc cattctcgga ggaagagtca
      961 aaactcttca tcccaagatt tttggtggta ttctcggacg ccgtgaaata gattcggatt
     1021 tgaaaaactt tgatgaatac aaaatacctc aaatagatct tgtaattgta gatctttatc
     1081 cgtttgagca aagtgtagct tcaggtgttt cccatgaaga gattatcgag aaaatagata
     1141 ttggaggtat atccctaatc agagcagctg ccaaaaatta taaagacgta gttgtaatcc
     1201 cttgtaaggg gctgtacgac aaatttttga caattctcga cgagcgtggt gcagagacct
     1261 ctttggaaga gcgccattat tttgccacag aagcttttaa catatcctca cactacgata
     1321 ctgcaatatt tacatacttc aatagagaag aaaaaataca agttttcaag aaaagtatct
     1381 tgacctcaag gcctcttcgt tatggagaga acccacatca gtctgccagt ttttacggca
     1441 gtgaggaaaa tttgcccgaa cagcttcacg gcaaagaaat ctcttataac aatctgctcg
     1501 atgttgaggc ggcaattgaa cttatctctg actttacaga cacaaccgta gcaatcctta
     1561 aacataataa tgcctgcgga tgtgcctcaa gagagacatt gaaagatgcg tggagtgcag
     1621 cacttgcctg tgatcctgtc tcagcttatg gtggtattat aatcacaaac cgcattatag
     1681 atgctgacac agcaaaagag atgaatactc tgttttgcga agttgtgatt gcccccgaat
     1741 atgaagaaga ggcacttaat attcttaaaa gtaagaagaa cagaatcata ctggtaaaca
     1801 gaggcgatct caggagtgac tataaattca gatctcttct tgaaggcgtt ctggttcagg
     1861 aaagagatac cgcattagag acaagagagt ctctaaaatg tgtcactgct aaggcacctt
     1921 cagaaagtga aattgaagat cttatatttg ccaataagat tgtaaaacat tccaaatcaa
     1981 atgctattgt gcttgcgaaa gacaggactc ttcttgcaag tggagttgga caaacttcga
     2041 gggtagatgc cctgaaacag gcaatagaca aagcccacag ttttggattt tccaccgaag
     2101 gagccgtcat ggcctcagac gcattcttcc cattttccga ctgcgttgag atagcacaca
     2161 aagcagggat taaatctgtc attcaccccg gtggctctat ccgggacaat gatagtattg
     2221 tgtactgtga tgaaaataat atctcaatgg taatgactgg tataagacat tttaaacact
     2281 gataacaagt aaatctaaat atggcatttt cttttttaac acaggatctt gcaattgatc
     2341 tgggcacggc aaacactgta ataatgatca atgataaaat tgtagtagac gaaccttcaa
     2401 ttgtagccat tgatcaaaat accggtaaac ctatagcttt tggtcaaaaa gcaaggatga
     2461 tgcacggcaa gacacatgca aatatcaaga caataaggcc tctccgtgat ggagttattg
     2521 cagactttaa tgcttgcgaa atgatgatac gaggattcgt aaagatgatt aactacaaac
     2581 actctctctt tactccgagt ctcagaatgg tagtcagcat tccat
//
LOCUS       DAOTQL010000192         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_30603_length_2616_cov_4.627489, whole genome shotgun sequence.
ACCESSION   DAOTQL010000192 DAOTQL010000000
VERSION     DAOTQL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30603_length_2616_cov_4.627489"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="P5293_08460"
     CDS             complement(<1..1018)
                     /locus_tag="P5293_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49964.1"
                     /translation="MESPFIFNKPVNVTEAIPRNNEVMKITELLTNKKNVLINEPPRC
                     GKYSLIQQVIDKLKHDKYPFKLCTIDLFNIRTEADLLRAIRVSLINCFANSDLEKEKL
                     EEDLMISFIHEMNEYKDLVLNLSEALASKFKTNIILYFIEFQEILNFENPDKTLKLLE
                     RVWRDHTGTTYLITGSQVNAMKQIFEKDSFFYRYIEKINFQQIPEHLFSEYIIKNFLK
                     AGRVVSKELAAQIYNFTKGHPYYTQQLAEISFYKTKGFMTDQILHESYEDLLQCHMHE
                     FKEKTSKLSLYQLNFLKAVIDGVTKFSETQTMEKYHFNSSANVVRLKEAVLKKEILSY
                     DNETW"
     gene            1257..1565
                     /gene="rplU"
                     /locus_tag="P5293_08465"
     CDS             1257..1565
                     /gene="rplU"
                     /locus_tag="P5293_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRR49965.1"
                     /translation="MYAIVDIAGQQFKVEKDKKIFVNRLHAEQGATLSFDKVLLIENE
                     GKVQIGSPYVEGASVKAKVLEHLKGDKVIVFKKIRRKGYDKKNGHRQCLTKILIEDIA
                     "
     gene            1589..1837
                     /gene="rpmA"
                     /locus_tag="P5293_08470"
                     /pseudo
     CDS             1589..1837
                     /gene="rpmA"
                     /locus_tag="P5293_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
     gene            2253..>2616
                     /locus_tag="P5293_08475"
     CDS             2253..>2616
                     /locus_tag="P5293_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRR49966.1"
                     /translation="MTNSDRCKLLTIVGARPQIIKAAAISRAIRNHFQESITEVILHT
                     GQHYDQNMSEIFFSELGVPTPHYNLNVGSGSHGSQTAKMIEGIENVLLAELPDVVLLF
                     GDTNSTLAGAIAASKLHIP"
BASE COUNT          777 a          407 c          512 g          920 t
ORIGIN      
        1 tccacgtttc gttatcgtag ctcaaaatct cttttttcag gactgcctct tttaggcgca
       61 cgacattggc tgaagagttg aaatgatatt tttccattgt ttgtgtttcg ctaaactttg
      121 taacaccgtc aattacggcc tttaaaaaat tgagctgata taaactcagt ttgctcgttt
      181 tttctttaaa ttcatgcatg tgacattgga gaagatcttc ataagattca tgcaaaatct
      241 gatctgtcat gaatcctttg gttttgtaaa acgatatttc tgcaagttgt tgagtataat
      301 atggatgacc ttttgtgaaa ttataaattt gtgcagcaag ttctttcgag actactcttc
      361 cggcctttaa aaaatttttg ataatatatt cagaaaacaa atgttcagga atttgctgaa
      421 aattaatttt ttcaatatat ctataaaaaa aactatcctt ttcaaaaatc tgtttcattg
      481 cattaacttg agagccggta atcagatatg tagttcctgt gtggtctctc caaactcgtt
      541 cgagtagttt gagggttttg tccggatttt caaagttgag aatttcttga aactctatga
      601 aatacaggat aatatttgtt ttaaatttgc tggccaaagc ttctgagagg ttgagtacca
      661 ggtctttata ttcgttcatt tcgtgaataa acgaaatcat cagatcttct tcaagttttt
      721 ctttttctaa atcagaattt gcaaaacaat ttatcaatga aacccgaata gctcttaaca
      781 gatctgcttc agtacgaata ttaaacaagt cgatggtgca aagtttgaaa ggatatttat
      841 catgtttcag cttatctata acttgttgta taagagagta ttttccgcat cttggaggtt
      901 catttatcaa cacatttttt ttattagtca ggagttctgt aattttcatt acttcattgt
      961 ttcttggaat tgcttctgtt acgtttacag gtttgttaaa aataaaaggg gattccattt
     1021 gtgctgattt ttaaattgtt ttcaaattcg aaaattgtag ttttgtctgc ttcattgttg
     1081 ttttggcata tatttacaaa tgtagcttta tacaaattta aaaatgtttt tgtttaataa
     1141 ataattaata atatctttgc attcctgatt tgggaagttg atgattgtta agctattgaa
     1201 taagagctgc ttaagcagcc gcttacagtc gaaatatata aatttttata ttttttatgt
     1261 acgcaattgt tgatattgca ggacagcaat ttaaagttga aaaagataag aagatctttg
     1321 tcaaccgtct tcatgcagag caaggtgcta ctttgtcttt cgacaaagtt ttgcttatcg
     1381 aaaatgaagg aaaggttcaa attggttctc cttatgttga gggtgcttct gtcaaggcta
     1441 aggtgctgga acatcttaaa ggtgataaag ttattgtttt caagaagata cgccgcaaag
     1501 gctatgacaa gaaaaatggt catcgtcagt gtttgaccaa aattttgatt gaggatatcg
     1561 cttaattaac aaaaacaaat tttaaattat ggctcacaag aaaggtgtcg gtagttctaa
     1621 gaacggccgt gaatcgcaca gtaaaagatt aggcgtcaag ttgtttggag gtcagtatgc
     1681 taaggctggc aacattatag ttcgtcagcg tggtacagtt cacaatcctg gtttgaatgt
     1741 cggtatgggt aaagatcata ctctgtttgc cttggttgac gggattgtta cttttaagaa
     1801 gaaagctgat aataagtcat atgtttctgt ttttcctaag tttttggata ctaagtcttc
     1861 agacgcttct ccagtttcag ttgaggcatc ggttgaggca tcagttgagg catcagttga
     1921 ggcttagact cattagtcaa aacttgggtt agggaaattt ggcaaattta tactgttagt
     1981 gtttgatgaa caacgattca gatttatttt atttaatttt aattgttgca aaatagacag
     2041 caacaggttg ttcctaaagg gttttagtcc attatgaact cagtttgagt gagttagtct
     2101 catgaaactg agttcatttt gtttcagggc acaggaggcc caaggggcag ccacccacct
     2161 gtcggagttt gtcatagtat gcagataatg agccctttaa aaaatatgac gaaaaataat
     2221 tcatttttta aaatacatat aaccagcata ttatgacaaa ctccgacagg tgcaaattac
     2281 ttacgattgt gggcgctcgt ccgcaaatta taaaagctgc agctatttcg cgagcgatca
     2341 gaaatcattt tcaggagagc ataactgagg ttattcttca tacagggcag cattatgacc
     2401 agaatatgtc agagattttt ttctcagaac ttggagttcc gacaccgcac tataatctca
     2461 atgttggctc aggttctcac ggaagccaaa ccgctaaaat gatagaaggc attgaaaatg
     2521 tgttgcttgc agaattacct gatgttgttt tgctctttgg tgatacaaat tcaactttgg
     2581 ctggagctat agcagcgtct aagttacaca ttccgg
//
LOCUS       DAOTQL010000193         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_30777_length_2606_cov_5.552724, whole genome shotgun sequence.
ACCESSION   DAOTQL010000193 DAOTQL010000000
VERSION     DAOTQL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30777_length_2606_cov_5.552724"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..841)
                     /locus_tag="P5293_08480"
     CDS             complement(65..841)
                     /locus_tag="P5293_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRR49967.1"
                     /translation="MNASNGGHTSGSGNYQWGETVSIEAIPNLGCRFIQWTGDLSNYG
                     NNHAFSIQLFFDITAMAVFQKNQPCRDATTADPLMNMALQGTPGNPVNGGRFTALRVR
                     KNKYGELETYQHQGLDLRVEQDTPSFSILEGVIIGIQNIFDPNVPWDEYKEVYGYLGP
                     DTYSAGNMVIVRSYVNGKTIDIVYMHLDNINSNLSVGSTIEPGTVIGVGGSTGAAKSP
                     SSGGPHLHLYVYENGVKKNVENYIYTKFDSNANITRTCNH"
     gene            complement(863..1729)
                     /locus_tag="P5293_08485"
     CDS             complement(863..1729)
                     /locus_tag="P5293_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49968.1"
                     /translation="MMKQNPIRFIKWVMIVVLACPTSCEKADISTQDLNNNDDLTRQI
                     KSTLISKYQGHIFTPDLSDYNYNVTTKASSHEQFTTSDLLWNKALYYSRDSVNYVAVE
                     IVKHNKTAIFSLYRQKPLSCFKRPVKTFLFYKSDDRTGNADMRVVTMINLNGCPTNPG
                     VFNPIDLSSFIGFIIHSDIDGRFISIKLYDGNKFYSVQLAKPSENNHKILYKLFMFSN
                     VETKSETSNEEENGLEIQLDELYCIADKNENKPPQDNEEAEWYDLDGGGHDHIEDMGG
                     EGGGGGSGDKQG"
     gene            complement(2182..>2606)
                     /locus_tag="P5293_08490"
     CDS             complement(2182..>2606)
                     /locus_tag="P5293_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49969.1"
                     /translation="DYYKACESKDMNAMLKAAEKFHSAYEGMNRVIKPFVKEMDTYHK
                     TLYVIYHKMLPENQFNEIASVMDQLIAEADAITKFPQDQLAKRLKDNTPKYYAISKEL
                     YDATVNVKKVLAEKNNDKSKAAIEQMHKTYQKLESVFE"
BASE COUNT          756 a          472 c          476 g          902 t
ORIGIN      
        1 ggaggtttgc tgaaacaaac agcgatacga taataaataa tatttttttc atattctatt
       61 aaaattaatg gttacaagtt ctggtgatat tggcatttga gtcgaattta gtataaatat
      121 agttttccac attctttttc actccatttt catagacata cagatgcagg tgaggtcctc
      181 cgcttgaggg gctttttgcc gctccggtgc taccacccac tccaataacc gtacccggtt
      241 ctatggtgct tccaacagac aagttgctat tgatgttgtc caggtgcatg taaactatgt
      301 caattgtttt cccattaaca taggaccgca ctataaccat gttgcctgcg gaatatgtat
      361 caggtcctaa atatccgtat acttctttat attcatccca cggcacatta gggtcaaaga
      421 tattttgaat accaattatc accccttcca aaatcgaaaa agaaggagta tcctgttcca
      481 ctctcaggtc caatccttga tgctgatatg tttcaagttc accatattta ttttttctta
      541 ccctaagagc agtaaacctt cctccgttga cggggtttcc tggagttccc tggagggcca
      601 tattcatcag tggatcagct gtagttgcat ctctacatgg ttggtttttt tgaaatacag
      661 ccatagctgt aatgtcgaaa aatagctgta tgctgaaagc atgattattt ccgtagttgc
      721 taaggtctcc tgtccattga atgaacctac atccaaggtt aggtattgct tcaatagata
      781 ctgtttctcc ccattggtaa ttgccggatc ctgaagtatg tcctccatta cttgcattca
      841 aagtaacagt aagagttgat gtttatcctt gtttgtctcc tgagccgcca ccgccacctt
      901 ctcctcccat gtcttcgata tgatcatggc cacccccatc taggtcatac cattctgcct
      961 cttcattatc ctgaggcggt ttattttcgt ttttatctgc tatacagtat agttcgtcga
     1021 gttgtatttc caacccattt tcttcttcgt tggaggtttc acttttcgtt tcaacatttg
     1081 aaaacatgaa aagcttatac aatattttat ggttattttc tgaaggtttt gccagttgta
     1141 cactataaaa cttattgcca tcataaagtt ttattgaaat aaatcgtcca tcaatatctg
     1201 aatggattat gaatcctata aaagaagaga ggtcaatagg gttgaatact ccgggatttg
     1261 tggggcagcc attaagattt atcattgtaa ccaccctcat gtcggcattt ccggttctat
     1321 catcagactt ataaaaaagg aatgttttga cgggtctttt aaaacaagac aacggttttt
     1381 gtctatacaa cgaaaagatg gctgttttgt tgtgtttcac aatttctacg gcaacataat
     1441 tgacgctatc tcttgaatag tagagtgctt tgttccatag cagatcagaa gtggtaaact
     1501 gttcatggga gctggccttc gttgttacat tataattgta atctgagagg tctggagtaa
     1561 agatgtgtcc ttggtatttg ctgataagag tcgatttaat ctgtctggtt aaatcatcgt
     1621 tgttattgag gtcttgtgtt gaaatatctg ccttttcgca agatgttgga caagccagaa
     1681 cgacaatcat tacccactta ataaagcgaa ttggattttg tttcatcata aaaagattga
     1741 caaaaaggaa tgaataaatt tttgaaatta aaatacgatc acaagtaatg tcatgtgaaa
     1801 tgaaatctct atattcgcta tgccgttttg caattttaat taatggtgtc gttactttgg
     1861 tgggtaataa tattcattag attaaaatca aaacggcttt aagttggcaa gacttagttc
     1921 cttgttttca atctaataac gaagaacaat ataagtaaat ttttctgaag tttgcaagca
     1981 aaatgctcat aatatgctca ataaaagtca gtaagcatag taaaagtgtg gcggagcaag
     2041 ggtttggaga agaaaatgaa ttttgcaaaa ggaagctaat gtcactgtga cagattagct
     2101 aacacaaaaa aatctgactt aaattatctt ttaagtcaga tttttaaatt agaattaaaa
     2161 ttataattaa atgcttgtca attattcaaa aacagattca agcttttgat aggttttatg
     2221 catttgttca atagctgctt tacttttgtc attatttttt tctgcaagta cttttttgac
     2281 gttgacagta gcatcataga gttcctttga gatggcataa tactttgggg tattatcctt
     2341 tagacgttta gcaagttggt cctgaggaaa ttttgttatt gcatctgctt cggcaatcag
     2401 ctggtccata acagaagcaa tctcattaaa ttgattttca ggaagcatct tgtggtaaat
     2461 aacatacaga gttttgtgat aggtatccat ctcctttaca aacggcttaa taactctgtt
     2521 catcccctca taggctgaat gaaacttttc agcagccttt agcatggcat tcatatcttt
     2581 agattcacaa gccttataat aatctt
//
LOCUS       DAOTQL010000194         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_30857_length_2601_cov_5.202671, whole genome shotgun sequence.
ACCESSION   DAOTQL010000194 DAOTQL010000000
VERSION     DAOTQL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30857_length_2601_cov_5.202671"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="P5293_08495"
     CDS             complement(<1..402)
                     /locus_tag="P5293_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HRR49970.1"
                     /translation="MINDSLKVNVQSEIGKLEAVLLHTPGAEVENMTPRNAQRALYSD
                     ILNLSIAQKEYEQVSGVLAKVCKTYEIKELLTSLLEIHTEREALIGKICIAEGVTDYF
                     EYLMNMPSKSLATVLIEGLPARMDTLTAFLKD"
     gene            complement(408..1499)
                     /locus_tag="P5293_08500"
     CDS             complement(408..1499)
                     /locus_tag="P5293_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HRR49971.1"
                     /translation="MNKNHYCIIMAGGVGSRFWPISRNYRPKQFLDILGLGKSFLQLT
                     FDRFNKIIPKENILIVTSDSYKDLVYEQIPEVLPENLLLEPFRRNTAPCIAYATYKLL
                     KKNPDATVVAAPSDHMITNEELFLTTVSNALKYAESHDVLMTLGIQPTRPETSYGYIQ
                     VNKAEPVDVDGNIAYKIKTFTEKPDLKLAKVFVDSGEFLWNSGLFIWNLKTIADQLEI
                     FQPEISGLFKAITDDYYTDRENKAIMSVYQECMNISIDYAVMEKTSKAVVYPASFGWS
                     DLGTWDSLFSYVDKDENNNYVSSKGDTLIEVKDSIVISEDDRLIVVKGLENYLIINSK
                     DALMICPRDDKSFKSILTDLTIKDLSKYQ"
     gene            complement(1512..1901)
                     /locus_tag="P5293_08505"
     CDS             complement(1512..1901)
                     /locus_tag="P5293_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRR49972.1"
                     /translation="MKNVQKRQELGKRAEEIALEFLEDKGLTFLEKNWRSGHREIDII
                     MKSLNERGGMILHIVEVRSLREPAVRQPFETVGFEKQRAVIAAARSFIFNYNLDCETQ
                     FDIVSVTFGEYGVKITYFPDAFKPTWR"
     gene            complement(2029..>2601)
                     /locus_tag="P5293_08510"
     CDS             complement(2029..>2601)
                     /locus_tag="P5293_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR49973.1"
                     /translation="MYMPFRYSYTYNGDVNNDSYSYNDLMFIPATKDQIRIKPATGDT
                     RTADQIWAEMDAFFNQDPYLKHRRGKYSERNGGMAPYVHSLDFNIAHDLIVKQANGKS
                     HTLRFSFDFYNFLNLLNKDWGIQETTVLGNQQYQYLVMTEKPSAANNYTPAFSTALDK
                     NKQVITDTFMDSVSSSSRWTMMFGIKYIFN"
BASE COUNT          787 a          546 c          437 g          831 t
ORIGIN      
        1 atccttgaga aatgctgtca aagtgtccat tcttgcaggc aaaccttcaa taagtacagt
       61 tgcgagagat ttggagggca tattcatcag atattcaaaa taatcagtca cgccctctgc
      121 tatacatatc ttcccaatca aggcctccct ctcggtgtga atctcaagaa gacttgtaag
      181 taattctttt atctcgtatg ttttgcaaac ttttgcgaga actccggaaa cttgttcata
      241 ctctttctgt gcaatcgaaa ggtttaaaat atcactgtat aaagctcttt gcgcatttct
      301 cggagtcata ttctccactt cggctcccgg agtatggagc agcactgctt caagcttgcc
      361 tatttcagat tgaacattta cttttaatga atcgttgatc ataaaaatta ttgatatttc
      421 gacagatcct taattgtaag gtccgtcaat attgacttaa atgatttgtc gtcacgcgga
      481 cagatcatca aagcatcttt tgaatttatt attaaatagt tttccaatcc tttgacgaca
      541 atcaatctgt cgtcttcaga tataactatg gagtctttaa cctcaattag tgtatctcct
      601 tttgaagaga catagttgtt gttttcatct ttatccacat aactgaataa agaatcccac
      661 gtacctagat ctgaccaacc gaatgaagcc ggatagacaa ctgccttgga agttttctcc
      721 atcactgcat aatcaatgga aatattcatg cattcctgat aaaccgacat aattgcctta
      781 ttctctctgt cagtgtaata atcatcagta atagctttaa aaaggcctga aatctcaggt
      841 tggaaaattt caagctgatc cgcaatggtc tttagattcc atataaaaag accggaattc
      901 cacaaaaact ctccactgtc aacaaaaacc ttggccagtt tcaaatcggg cttttcagta
      961 aaagtcttga ttttataagc aatatttcca tcaacatcta ccggttctgc cttgttaacc
     1021 tgtatataac catatgatgt ttctggcctt gtaggttgaa tacccaatgt catcaacaca
     1081 tcgtgggact ccgcatactt gagtgcatta cttactgttg taaggaaaag ctcttcatta
     1141 gttatcatat gatctgacgg agcagcaaca actgttgcat ctggattctt tttcaaaagt
     1201 ttgtaagtcg cataagcaat acagggtgcg gtgtttcttc tgaaaggctc aagaagcaga
     1261 ttttcgggaa gaacttcagg aatctgctca tatacaagat ctttataact gtctgaggtg
     1321 acaattaaaa tgttttcctt tggtatgatt ttgttaaacc tgtcaaaagt aagctgaagg
     1381 aaagacttgc cgagtccaag aatatcgaga aactgcttgg gcctgtaatt tcggcttata
     1441 ggccagaaac ggcttcccac accgccggcc attattatac agtaatgatt tttattcata
     1501 tattatatta attatctcca tgttggttta aaagcatcgg gaaaataagt aatttttact
     1561 ccatattccc caaacgtcac agaaactatg tcaaactgag tttcacaatc caaattgtaa
     1621 ttgaatatga acgaccttgc agctgcaatt actgctctct gcttttcaaa ccctactgtc
     1681 tcgaatggtt gcctgactgc tggttccctg agcgatctaa cctctacaat gtgtaaaatc
     1741 atgcctcccc gctcgttcaa actcttcata ataatatcaa tctccctgtg accactcctc
     1801 cagtttttct ccaaaaaagt caacccctta tcctcaagaa actccaacgc aatctcctca
     1861 gctcttttcc ctaattcctg tctcttctgc acatttttca tctctgctcc acaaattcaa
     1921 tcaattatat cataacaaat ataagaaaca taattggctt gaccaaagta taatagaaaa
     1981 gagggtcccc aataattgga tgaccctctt taatatatta gtcaaatgtt agttaaaaat
     2041 atactttata ccaaacatca tagtccatct tgacgaagaa gaaacgctat ccatgaaagt
     2101 atcggtaatt acttgcttgt ttttgtccaa tgctgtagag aaagctgggg tatagttatt
     2161 tgccgcagaa ggtttctctg tcataaccag atattggtac tgctgattgc ccaaaacagt
     2221 tgtttcctga attccccagt ccttgttgag aaggttaagg aaattgtaga aatcaaaaga
     2281 gaatcttaaa gtatgtgact tgccatttgc ctgcttaaca ataagatcgt gagcaatatt
     2341 gaaatcaagt gaatgaacat aaggtgccat tccgccgtta cgctccgaat acttacctct
     2401 tcgatgtttc agatatgggt cctgattgaa gaatgcatcc atctctgccc atatttgatc
     2461 tgctgtacga gtatcgcctg tggcaggttt gatacgaatc tgatcttttg ttgcaggaat
     2521 aaacatcaaa tcattgtatg aatacgaatc attatttaca tcaccattat atgtataaga
     2581 atatctgaaa ggcatataca t
//
LOCUS       DAOTQL010000195         2582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_31199_length_2582_cov_3.839335, whole genome shotgun sequence.
ACCESSION   DAOTQL010000195 DAOTQL010000000
VERSION     DAOTQL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31199_length_2582_cov_3.839335"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="P5293_08515"
     CDS             <1..205
                     /locus_tag="P5293_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HRR49974.1"
                     /translation="GQVWITLQTHKNIVAVASLKDLAAIVLVKGFVPEAETVEAAMAE
                     GIPLLGSDLGAFEISGKLYDLLK"
     gene            202..921
                     /locus_tag="P5293_08520"
     CDS             202..921
                     /locus_tag="P5293_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRR49975.1"
                     /translation="MIKADLHLHSVLSPCGDIEMTPSFIIRRAREKNLGMIAVTDHNS
                     TRGYEEIARIGRREGVAVMCGAEITTREEVHVVAIVNDADREKFQLWLDRYLPKIPNR
                     EDIFGYQLVVNEFEEVIYQEPYLLLSALDRSIDQVEEYIHSLGGIFIPAHIDKFQNSI
                     TSQLGFVPQGIRADALEISFRSDLQLLLKNYPWIENYTFVRSSDAHYPDDFGDVFSVF
                     DIQEPSFEALKEALKLKKVIL"
     gene            918..1466
                     /locus_tag="P5293_08525"
     CDS             918..1466
                     /locus_tag="P5293_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRR49976.1"
                     /translation="MNDLSMHIIDIIQNSLRAGATLITVTIEEDREDDLLVITVEDNG
                     CGMSSEQVAKLNDPFFTSRTTRRVGMGIPLLQQTAEQSGGELEIKSEPGIGTTVKASF
                     GYSHIDRPPLGDVANAFMLMVSANPEIDFILRYVVGNADYLFNTVEIKKVLEGIPVNE
                     PSVIRALTEMIRENIEDLKKKK"
     gene            1571..1972
                     /locus_tag="P5293_08530"
     CDS             1571..1972
                     /locus_tag="P5293_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HRR49977.1"
                     /translation="MNKIKSIDDLRKIKQDLENGMNIREKSNSPESLVQIRVAMATCG
                     IAAGARTVMNTLIEKIEKDKVPAVVTQSGCMGYCYAEPTVEVRIPGKDPVVFGYVDSK
                     KAIDIVEKYIVKGELLDGIIPVNYQTINDKI"
     gene            1977..>2582
                     /locus_tag="P5293_08535"
     CDS             1977..>2582
                     /locus_tag="P5293_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRR49978.1"
                     /translation="MAQYRMHLLVCGGTGCRASQSEKIAENLNEEIKNIGLENEVQVV
                     ITGCFGFCEKGPIVKVIPDNTFYTQVKPEDCKEIVNEHVLKGRRVMRLLYVDPATKQH
                     VSDSKHMGFYQKQLRIALRNCGFIDPENIDEYIARDGYAALGKALTEMTPQQVIDLIK
                     KSGLRGRGGGGFPTGLKWEIAAANKADQKYVVCNADEGDPGA"
BASE COUNT          781 a          411 c          618 g          772 t
ORIGIN      
        1 ggggcaggtt tggattacat tgcagactca caaaaatatt gttgcagtag cttctttgaa
       61 agatcttgct gccattgttc ttgtcaaggg atttgtaccg gaggcagaga ccgtcgaagc
      121 cgcaatggca gagggaattc ctcttttggg cagtgacctt ggagcatttg aaatctctgg
      181 aaaactgtat gaccttttaa aatgattaag gcagatcttc atcttcattc tgtactgtct
      241 ccctgtggtg atattgaaat gactccctct tttataatcc gaagagcgag ggagaaaaat
      301 cttgggatga ttgctgttac tgatcataac tccacgaggg gttatgagga gatagcgagg
      361 ataggaagac gcgaaggtgt tgcagtgatg tgtggcgctg agattactac cagagaagag
      421 gtgcatgttg tggctattgt gaatgatgct gacagggaga agtttcaact ttggcttgac
      481 agatatctcc caaaaatacc caacagagaa gatatttttg ggtatcagct tgtggtgaat
      541 gaatttgagg aagttatcta tcaggaacct tatcttttgt taagtgcact tgatcgttca
      601 attgatcagg tggaggagta cattcattct ttaggtggta tctttattcc tgctcacata
      661 gacaagtttc agaattcgat aacaagtcag ttgggctttg tgccacaggg aatcagagcc
      721 gatgcactgg aaatatcttt caggtcagat cttcaacttt tattaaaaaa ttatccctgg
      781 attgagaatt atacttttgt cagatcttcg gatgctcatt acccggatga ttttggagat
      841 gttttttcgg tgtttgatat tcaggaacct tcttttgaag ctttgaagga ggctcttaaa
      901 ctaaaaaagg tcatcttatg aatgatttgt cgatgcatat tatagatatt attcaaaatt
      961 ccctcagggc aggagcaaca ttgataacgg ttactattga ggaagatcgc gaagatgacc
     1021 tgcttgttat tacagttgag gataatggtt gtggaatgag tagtgagcag gtagcaaaac
     1081 tcaatgaccc tttttttaca tcgaggacaa caagaagagt agggatggga attcctctcc
     1141 ttcaacaaac tgcggagcag agcgggggag agcttgagat taaatcagaa cctgggattg
     1201 gcacaactgt caaagcttct tttggatact cacatattga caggcctcca cttggagatg
     1261 tagcgaatgc atttatgttg atggtttctg ccaatcctga gatagatttt attctaaggt
     1321 atgtagtggg gaatgctgat tatcttttta atacagttga aataaaaaag gttttggaag
     1381 gtatacctgt caatgaacca tctgttataa gggctcttac tgaaatgatt agagagaata
     1441 ttgaagatct taaaaagaag aagtgattac aaatgttaag taaaaagtta atatttattt
     1501 gtttttgatt taattattag tacttttgag catcatttaa atagtttaac aatcaaataa
     1561 ccaattattt atgaacaaga ttaaatctat tgacgatttg cgcaagatta agcaggatct
     1621 tgagaacgga atgaatatcc gtgaaaagag caattcgcca gagtctttag ttcagattcg
     1681 tgtggctatg gctacctgtg gtatagctgc gggcgccaga actgtcatga atactcttat
     1741 tgaaaaaata gaaaaggaca aagttcctgc tgtggttact caaagtggat gcatgggtta
     1801 ttgctatgca gagcctacag tcgaggtcag gattccgggt aaggatcctg ttgtctttgg
     1861 ctatgtggat tccaaaaagg caattgacat cgttgagaag tatatcgtta aaggcgagct
     1921 cctcgatggg ataataccgg ttaattatca gaccattaac gataaaatat agtgatatgg
     1981 cacaatacag aatgcacctt ttagtatgtg gcggaactgg ctgccgtgcg tctcagagtg
     2041 aaaaaattgc tgaaaacctt aatgaagaga ttaaaaatat tggtctggaa aatgaggttc
     2101 aggttgtcat aacaggctgt tttggtttct gtgaaaaggg tcctatagtt aaagttattc
     2161 ctgacaatac tttctataca caagttaagc ccgaagattg caaagaaata gtaaatgagc
     2221 atgttctcaa aggcagaaga gtaatgaggc ttctttatgt agatccagct accaaacagc
     2281 atgtttctga ttccaagcat atgggtttct atcaaaagca gttacgtata gcactgagaa
     2341 actgtggctt tattgatccg gaaaatattg atgaatatat agctcgtgat ggctacgctg
     2401 ctcttggcaa ggctcttacg gaaatgactc cacaacaggt aatagacctg attaaaaaat
     2461 ccggattgag aggaaggggc ggcggaggat ttcctacagg tcttaagtgg gaaattgcgg
     2521 ctgctaacaa agctgaccag aaatatgtag tatgtaatgc cgacgagggt gaccctggtg
     2581 ct
//
LOCUS       DAOTQL010000196         2575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_31339_length_2575_cov_3.258333, whole genome shotgun sequence.
ACCESSION   DAOTQL010000196 DAOTQL010000000
VERSION     DAOTQL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31339_length_2575_cov_3.258333"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1452)
                     /locus_tag="P5293_08540"
     CDS             complement(64..1452)
                     /locus_tag="P5293_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49979.1"
                     /translation="MKQHKIVTTVVSYIFCILYLTSCFEVPDRDIKPFYVYSYNDENS
                     NIVAEIYIKTKDNTSFESISASIPYLKYNKSWLMILTMDDCKQDAYCRVWAAINGKPL
                     PVSESVYYNAANLLYGDMPLNVYYLNKTLGSTDGAGNEVRFNFTTTLSPEENFMNAYP
                     SPTGFNMFSGLIWDNVREIVNYDNGIAFHNVKADDESDVEKIKDHFALAQEIIIKQLN
                     GRGCKTLAEPDGNKKYIEAASNYPPIQIMTAQSQAEKLFPGKVNSSIYKNIIQRDFYR
                     PCEWLDNINKILSKSPENREAFCVGIHGMTGRGQIDTLALLNDLFGKDGDDSVWFTSL
                     EEYYEYSEYRLNTTIKKSYSNDTLKLTVSIPSQQYFYYPSITINLTNVSMSNVIDISS
                     SKMVSGMSYAQFGNGIMINIDCRKHLLEHAVYFVEKYEDNPSKSNRDDALFFVNRLKT
                     SQAKQALLQRLN"
     gene            complement(1456..>2575)
                     /locus_tag="P5293_08545"
     CDS             complement(1456..>2575)
                     /locus_tag="P5293_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRR49980.1"
                     /translation="NTAEEMEEAFCGRTSEHVYSRLTNPTVQYFEQRVQEITGALSVT
                     ALNSGMAAISNAIMTLAWSGSNIVTSPHLFGNTYSFFYSTLADFGVEVRFCDMTNIDE
                     VRKFIDKNTAALFLEIITNPQLEVADLKALSAVARSSKIPLVADTTAVPFHIFSSREF
                     GVDIEIVSSTKYISGGATSLGGLIIDYGTADFSSSPKLRRVAISKEAFTYKLKKEVMR
                     NFGAYMTPQVAYMQTLGLETLEVRFDKEASTTLELAERLSSLKGIESVNYTGLKSSSF
                     YAISLRQFGSKPCAFLTFDLPSKEKAFSLINNLKLIRRATNLFDNKTLAIHPATTIFG
                     TFTDEQRAMMNISQKSIRLSVGLENAETLYEDIRQSLN"
BASE COUNT          780 a          459 c          481 g          855 t
ORIGIN      
        1 ccgcataaaa aacgcgggct gcaaagttac gataatttat tgtaaatgca agagcccaaa
       61 gcactaattt aatctttgaa gcaatgcctg tttggcctga gaagttttaa gcctgtttac
      121 aaagaaaagt gcgtcatcac ggttggattt ggaaggatta tcttcatatt tttctacaaa
      181 atatacagcg tgttctaaca aatgtttgcg acaatctata tttatcatta taccatttcc
      241 aaattgggca tatgacattc cggagaccat ctttgaggaa gaaatatcta tgacattaga
      301 catcgaaacg tttgttagat taattgttat tgaaggataa tagaaatact gttgtgaagg
      361 aatggataca gttagcttaa gagtatcatt tgaataggat tttttaattg ttgtgttaag
      421 tcggtattcg gaatattcat aatattcttc cagagaagta aaccacactg aatcatctcc
      481 atcttttcca aacaagtcat tcaataaagc aagagtatct atctgacctc ttccagtcat
      541 gccatgtatg ccaacacaaa aggcttctct gttttcaggg gatttcgaaa ggattttatt
      601 gatattatcg agccattcac aaggtcggta aaaatctctt tgtattatgt tcttgtaaat
      661 acttgaattg acttttcctg gaaataactt ttcagcttgt gattgagccg tcataatttg
      721 tattggaggg taattgctgg cagcttcaat gtatttttta ttcccgtcag gctctgccaa
      781 agttttacat cctcttccgt tgagttgttt aatgattatc tcttgagcta atgcaaagtg
      841 atcttttatt ttttcaacat cggattcatc atctgcctta acattgtgaa aagcaatgcc
      901 attgtcgtaa tttactattt ccctgacatt atcccatatc aacccactga acatattaaa
      961 tccagttgga gaaggataag cattcataaa attctcttcg ggtgacaggg ttgtcgtaaa
     1021 gttaaatcta acttcatttc cagcgccatc tgtcgatccg agagttttat tcaaataata
     1081 gacattcaat ggcatatcac cgtataatag atttgcagca ttataataga cagattcact
     1141 tacaggtaaa ggtttcccgt ttattgcagc ccaaaccctg caataggcgt cttgtttgca
     1201 atcatccata gttaatatca ttaaccagga tttattatac ttaagatatg gaatacttgc
     1261 tgaaatactt tcaaatgagg tgttgtcttt tgtttttata taaatttcgg ctacaatatt
     1321 gctattctca tcattgtatg aataaacata aaaaggttta atatctctat caggaacttc
     1381 aaaacatgaa gttaaataaa gaatgcagaa tatatacgat actactgttg taacaatttt
     1441 atgttgtttc ataacttaat tcaatgactg tcttatgtcc tcatataacg tttccgcatt
     1501 ttcaaggccg acggatagac gtatgctttt ttgagagatg ttcatcattg cccgctgttc
     1561 atctgtgaaa gttccgaaaa tggttgttgc agggtgaata gcgagtgttt tgttgtcaaa
     1621 aagatttgta gctcttctga ttaatttcag attattaatt aatgaaaaag ctttctcttt
     1681 ggatggaaga tcaaatgtta aaaatgcaca tggtttacta ccaaactgtc taaggctgat
     1741 tgcataaaac gagctgctct tcagtccggt ataatttaca ctttctattc ctttaagtga
     1801 ggataggcgt tcagcaagtt caagggttgt tgaggcttcc ttgtcaaaac gaacttcaag
     1861 tgtttccagt ccaagtgtct gcatataggc aacttggggt gtcatgtatg ctccaaaatt
     1921 tctcattacc tcctttttaa gcttgtatgt gaaggcctct ttactaatgg ctacacgcct
     1981 aagtttggga gaagaactaa agtctgcagt accataatca ataataagcc ctccaagtga
     2041 ggtagctcct ccggaaatat attttgtgct ggaaactatt tctatgtcta caccgaattc
     2101 tctggatgag aaaatgtgaa atggcactgc cgtagtatct gccaccaaag ggattttaga
     2161 acttcgtgca acggcagaaa gtgccttgag atctgcgact tcaagttggg ggtttgtaat
     2221 aatctctaaa aaaagtgcgg cagtattttt atcaataaat tttctgactt cgtcaatatt
     2281 tgtcatgtcg cagaatctga cttcaactcc aaaatcggca agtgtagaat aaaagaatga
     2341 gtaagtgttt ccaaagagat gtggggacgt aacaatattg gatcccgacc acgcaagagt
     2401 cattatggca ttactgatgg cagccatgcc ggaattaagt gctgtaacac taagggctcc
     2461 agtaatttct tgaactcttt gttcaaaata ttgaacagta ggattagtta gtcgagaata
     2521 gacgtgctcg gaagtccgtc cacaaaaagc ctcttccatc tcttcagctg tatta
//
LOCUS       DAOTQL010000197         2564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_31521_length_2564_cov_3.706257, whole genome shotgun sequence.
ACCESSION   DAOTQL010000197 DAOTQL010000000
VERSION     DAOTQL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31521_length_2564_cov_3.706257"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..910)
                     /locus_tag="P5293_08550"
     CDS             complement(182..910)
                     /locus_tag="P5293_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HRR49981.1"
                     /translation="MIRIIYYIILAYFLLGAVGFIFINRKKERTVARNNWVKFLTYFI
                     IINIVFFCIVLGPVFFRSVAIIIIISGCLELSRLYVKSGHFHRQVFYFSLLIFLIISA
                     GFFVFSGMEKELVLYVFLILSIFDSFSQISGQLWGKRKLMPTISPNKTWGGLAGGTLI
                     ALISGLLLKNLLYISSIEAIALTVNIILSAFVGDMASSFYKRQYKVKDFSNLIPGHGG
                     FLDRFDSLIASGAWIALLRIVANL"
     gene            complement(920..1717)
                     /locus_tag="P5293_08555"
     CDS             complement(920..1717)
                     /locus_tag="P5293_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRR49982.1"
                     /translation="MFFLSSFKYIYLWLPVIGFIIAFFASMTGGGGGFFFLPVLTIFF
                     GVPARIAVATSLAATLPVCLASSFGHYRKGNVNLKVGLIFSISGIIGALSGAAITSRI
                     NSKQLKICFGIYSIIIAFHMIWTTRKKKKAELNGTCLPELKKIRKITRGSFYGFLSGI
                     ITGTFGTSGTAPVLTGLFDMRIPLKMVVGTSVMIVFVNTISALGGHILVGDIDMTLVV
                     FLTLGTIIGAIVGSKTFAGVELEKAETNIRQWYAIAMIAFGIMMVIY"
     gene            complement(1725..2315)
                     /locus_tag="P5293_08560"
     CDS             complement(1725..2315)
                     /locus_tag="P5293_08560"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRR49983.1"
                     /translation="MKEKQLFNIPNILTLYRIAVFPLILGFILSGKENLFAIFVIINL
                     LTDVADGFIARKFKLETEFGAKIDSFADDLTYLAAIIGIFVFKWEDFMPYMTSFLIFV
                     GFLIIPIIVYLIRLGKIPSFHLYMTKIGGYIQGLFFFCLFTIGFVPSLYYFMIVWGIL
                     GSLEHLAIQFYLKKEIRSNVKGLYWVLKEKKIEKQI"
BASE COUNT          927 a          459 c          388 g          790 t
ORIGIN      
        1 aagaaagggg aatacagttg tagaaagtgt tgctgcaaag tgagcaaatt ttcttgtcat
       61 ctcacctttc agttcatatc ttctatagat taattctgta aaaatcagta aacaggcaat
      121 tgcaaccaga aaaataattg ttaataaaat ttgattgttc atatctctta ttttaaggca
      181 tttacaaatt agcaacaatt cttaacaaag caatccatgc tcctgatgca attaagctgt
      241 caaatctgtc taagaatccc ccgtgcccag gtattaaatt actgaagtct ttgactttgt
      301 attgtctctt gtaaaatgaa cttgccatat caccaacaaa agctgaaaga ataatattta
      361 cagtaagtgc tatagcttct atggaagaga tatataaaag attttttagc aagaggccac
      421 taattaatgc aataagagta ccacctgcaa ggcctcccca tgttttattg ggacttattg
      481 tcggcataag ttttctttta ccccatagtt gtccggatat ttgactgaag ctgtcaaaaa
      541 ttgacaatat taaaaataca tataaaacaa gttctttctc cattccgctg aagacaaaaa
      601 atccggcaga tataattaga aaaattagca gtgaaaaata aaacacttgt ctatgaaaat
      661 gtcctgactt aacatatagt cttgataatt ccagacagcc tgaaatgatg ataattatgg
      721 caactgatct gaaaaataca ggacccagca caatgcagaa aaagactatg tttattatta
      781 taaagtatgt cagaaacttt acccaattat ttcttgcaac tgttcgttcc ttttttctgt
      841 tgataaatat aaatccgaca gctcctaaaa ggaaatatgc caatattata taatagataa
      901 ttctaatcat atcgatgaat taataaataa ccatcattat accaaatgca atcattgcta
      961 ttgcatacca ttgtcttata ttggtttcgg ctttttccag ttcgacaccg gcgaaagttt
     1021 ttgaaccgac aattgcacca ataattgttc ccaaagtaag aaatactacg agagtcatgt
     1081 caatgtctcc aaccagaata tgccctccca atgcagaaat cgtattgaca aaaactatca
     1141 tgacagaagt gccgacaacc atttttaatg ggattctcat atcaaacaat cctgtaagaa
     1201 caggtgcagt cccacttgtc ccaaatgttc ctgtaattat tccggaaagg aaaccataaa
     1261 atgagcctct tgtaattttc ctaatttttt ttaattcggg caggcaagta ccattcaatt
     1321 cagctttctt tttttttctt gttgtccaaa tcatatgaaa agctattata attgaataga
     1381 tcccaaaaca tatttttaat tgtttcgaat ttatccgact ggttatggca gcaccagata
     1441 aagctcctat aatgcctgaa attgagaaga tcaacccaac cttaagattt acgttacctt
     1501 ttctgtaatg cccaaatgaa ctggcaagac aaactggcaa agtggctgca agagatgtag
     1561 ctactgctat tctcgctggc actccaaaaa aaattgtaag aacaggaaga aagaaaaaac
     1621 ctccacctcc ccctgtcatt gaggcaaaaa aggcaataat aaaccctatt accggcagcc
     1681 aaagataaat gtatttaaat gatgacagaa aaaacatatt aatactaaat ttgcttctct
     1741 atttttttct ccttaagtac ccaatataaa ccttttacgt ttgatcgtat ctcttttttc
     1801 aaatagaact gaatagctaa atgctccaaa gaacccaata tcccccaaac aatcatgaaa
     1861 tagtataaag atgggacaaa gccgatggtg aaaagacaaa agaaaaaaag tccctgaatg
     1921 tatcctccga tttttgtcat atacagatga aaactcggaa ttttaccaag tcttatcaga
     1981 taaacaataa ttgggataat caaaaatcca acaaaaatca agaaacttgt catataaggc
     2041 ataaaatctt cccacttgaa aacaaagatg cctatgattg cagcaagata tgtcaaatca
     2101 tcagcgaatg aatctatttt tgcaccaaat tcagtctcaa gcttgaattt tctggcgata
     2161 aaaccatccg caacatctgt cagtaagttg attattacaa aaattgcaaa taaattttct
     2221 tttccactca atataaatcc caaaatcagg ggaaatactg ctattctata tagagttaat
     2281 atatttggaa tattgaataa ttgtttctcc ttcatgaaat atttgctagt tgttacaaat
     2341 atatgaattt atataataaa ttaccactac agtcttggag taataggtaa tttagtaaat
     2401 ttgaattact ttataaaata tgttgtaatt atggaattta ggataagaga agctatgccg
     2461 gatgatgtcg gcattatact tggatttatt tgtaaactag cagaatatga gaaattgagg
     2521 gatgaagtta ctgctaccaa ggagatactg tatgattcac gctt
//
LOCUS       DAOTQL010000198         2558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_31624_length_2558_cov_3.687175, whole genome shotgun sequence.
ACCESSION   DAOTQL010000198 DAOTQL010000000
VERSION     DAOTQL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31624_length_2558_cov_3.687175"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="P5293_08565"
     CDS             complement(<1..890)
                     /locus_tag="P5293_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR49984.1"
                     /translation="MKKNRIKRRSLFMGIVMMIVVGSCQKNEFEYENVSGILTFRFQL
                     LDGRVFDCTVDQPAKTIQNDLDSLLFGTSSATLAKIKPIFTTTIGAQVFVNGTEIKSG
                     ESEIDLNQPIKITTRFKDAQREYSVKAFVEKVDHSQTSGAKINTDMRMTGLPSFNSYS
                     AAWFNNKLYILGAYYPNGTASSGTAYYELYSSDDGGLWTKVETNPKIIGGFGAELAVM
                     NGKLYAIGGVRMFCKDINGTAPDASSTSVWRMMSTTNGTDWTDCTSGQVGNPLGRPFP
                     QIVVHNGQLYLRRGKSYSFG"
     gene            complement(1075..>2558)
                     /locus_tag="P5293_08570"
     CDS             complement(1075..>2558)
                     /locus_tag="P5293_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRR49985.1"
                     /translation="NKINIKKSKDSSGRVVLPHKLLPASISSCKTKADSLLATKKLIS
                     QTINFLESGQTAKALFAINEAISFCPKDDIRTNAIATAYYALVQIKAGCHSKSVTSLN
                     KCDSLFRKLGDVYLLAFHYNNLGLFNQKFLSQKQAEKYFLRALAFSRAIGDSSTIAAT
                     ALNNLAKGEGNASVKIGYLKEAISINKKAKRTLSLGENYNNMAYQYISLGDLSKAQKY
                     LDSALVIGQELNSQEILINNHDYRSILYAKKGNYKAAYEETLKREEAQKKLAEGENIS
                     DIDELIANRLLTKKEYEINLQKKENDIRRLNFSLVITASLLIITVLSLLYIFFYINNK
                     RRIQELESKKIAAEQEVKYTQAELTNLSTYINSRNEILCNIQSSLSKAQKMDEKEVYS
                     ELRRVNLYIRNLQTRNEDVESVLNKIRGINESFIKRLSELHPDLTKNDRNIALLLRAN
                     LSTKQIASLLDCSPKSVNMARYRMRQHLNIDSDINLVKYLKSI"
BASE COUNT          705 a          452 c          479 g          922 t
ORIGIN      
        1 attccgaagc tatatgactt ccctctgcgc aggtataatt gtccgttatg tacaacaatt
       61 tgagggaaag gcctgccgag tggattgccc acttgtccgg aagtacagtc ggtccaatct
      121 gttccattgg ttgtagacat cattctccat acgcttgttg aagaggcatc aggagctgtg
      181 ccatttatat ccttgcaaaa cattctgaca cctcctatgg catataattt tccattcatc
      241 actgccagtt ctgctccaaa acctccaatt attttaggat ttgtttctac tttggtccag
      301 aggcctccgt catctgaaga gtacaattca tagtaagcag tcccggatga agctgttcca
      361 tttggataat atgctcccag tatatacagt ttattgttaa accaggcggc actgtaagaa
      421 ttaaaagaag gtaggccagt catcctcatg tcggtattta tttttgctcc agatgtttga
      481 ctgtgatcta ctttttccac aaatgcttta acggaatatt cacgctgagc gtctttgaaa
      541 cgcgttgtaa tcttgattgg ctgattaaga tcgatttcac tttctccact tttgatttct
      601 gtgccattga caaaaacctg agctccaatg gtagtggtga agatgggttt tatcttggct
      661 aaagtggcag aacttgtgcc aaataaaagg ctgtcgagat cgttttggat tgttttagca
      721 ggctgatcaa ccgtacagtc aaaaacacgt ccgtcaagaa gctgaaatct gaatgtaagt
      781 atgccagaga cattttcata ttcaaactca tttttctgac aggaaccgac tacgatcatc
      841 attactattc ccataaaaag cgaccttctt ttgatcctgt tttttttcat aatggttatt
      901 aagttttgat tggttaatag ttttgtgtca aatttcaaaa aagtaattat ttgatactcc
      961 attattacct taaataaata tctacaaaat tgaaatttgg agaaaattat atctacaaaa
     1021 tattttttaa ctattttgtt tttaggatat tgaatttaaa gatataatct acaattatat
     1081 tgacttgaga tattttacca aattgatgtc actgtcaatg tttagatgct gtctcatacg
     1141 atatcttgcc atattgacac ttttgggtga gcaatcgagg agagatgcta tctgttttgt
     1201 tgatagattg gctctaagaa gcagagcaat gtttctgtca ttttttgtaa ggtcaggatg
     1261 taattcagag agtcgtttga taaaggattc gtttatccct cgtatcttat tcaggacaga
     1321 ttctacgtct tcgtttcgag tctggagatt tcttatgtag agattaactc ttctcagctc
     1381 agaatacacc tctttttcat ccatcttttg agccttgctc aatgagctct gtatattgca
     1441 taaaatctca ttgcgactgt ttatatatgt ggagagattt gttaattccg cttgtgtata
     1501 ttttacctct tgttctgcag caattttctt tgattcaagc tcctgtatgc gacgtttatt
     1561 attaatgtaa aaaaatatat atagaaggga aagaacagta attattaaca gagaagcggt
     1621 aatgactaat gaaaaattga gtcgtctaat gtcattttct tttttttgta aatttatctc
     1681 atattccttc tttgtcaaca acctgttagc gataagctca tctatgtcac tgatgttttc
     1741 tccttcagcc agtttctttt gtgcttcttc tcttttgagt gtctcttcat aagcagcttt
     1801 atagtttcct tttttggcgt ataatataga tctgtagtca tgattattaa ttaggatctc
     1861 ttgagaattc agttcctggc ctattactaa tgctgaatca agatattttt gagccttgct
     1921 caaatctccg agagagatat attgatatgc catattgttg taattttctc caagagaaag
     1981 agttcttttt gcttttttat taatactaat tgcctctttg agatatccaa tcttgactga
     2041 agcattgcct tctccttttg ccaggttgtt gagagctgta gctgcaattg tagaactatc
     2101 cccaattgct ctactgaagg ccaaagctct tagaaaatat ttttcggcct gcttttgtga
     2161 aaggaatttc tgattgaaca aaccaagatt gttataatga aaggctagta gatagacatc
     2221 tccaagcttt ctgaataagg agtcacattt attgaggctc gtaactgatt ttgaatggca
     2281 gccagcttta atctgaacta gagcataata agctgttgct atagcattgg tccggatatc
     2341 atcttttgga caaaatgaga tggcttcatt aattgcaaaa agagccttag ctgtttgccc
     2401 actttcgaga aagttaattg tttgggagat aagttttttt gttgccagaa gactgtcagc
     2461 ctttgttttg caggaggata ttgatgcagg gagcaatttg tggggcaaaa cgactctgcc
     2521 cgaagagtcc ttggattttt taatattaat cttgttaa
//
LOCUS       DAOTQL010000199         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_32031_length_2537_cov_4.609992, whole genome shotgun sequence.
ACCESSION   DAOTQL010000199 DAOTQL010000000
VERSION     DAOTQL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32031_length_2537_cov_4.609992"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..657)
                     /gene="nadD"
                     /locus_tag="P5293_08575"
     CDS             complement(97..657)
                     /gene="nadD"
                     /locus_tag="P5293_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRR49986.1"
                     /translation="MKIALYFGSFNPMHVGHLTICRYLMENCDIDELRLVVSPANPLK
                     DESNEKSAEERLKHVKKVAANLEKEALQKGLKCKIHVSSIEFKLPKPLYTIDTLRTIT
                     EREKGNKLILIIGADNLAIIEKWNRWKELLNEFEVWVYPRKGYDIESLCKKYGVKPLD
                     APMIDISSSEIREAELSGNDMSAYKV"
     gene            complement(654..1223)
                     /gene="gmk"
                     /locus_tag="P5293_08580"
     CDS             complement(654..1223)
                     /gene="gmk"
                     /locus_tag="P5293_08580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRR49987.1"
                     /translation="MKKENRAMIFSAPSGAGKSTIVSHLMKKFPVLGFSISATSRQPR
                     ENEVNGREYYFLSTEEFRKKIANNEFVEYEEVYKGYYYGTLKSEVERLWNEGKVIVFD
                     IDVKGGLNLKKLYGDAALAVFIKPPSIEVLRERLVKRGSEDSASLERRIKKAEYELTF
                     ESQFDKVLVNDSLEQCLKEAEQITEEFLS"
     gene            complement(1207..1980)
                     /locus_tag="P5293_08585"
     CDS             complement(1207..1980)
                     /locus_tag="P5293_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRR49988.1"
                     /translation="MKSSVLEKNTWKSINNSNIKIALLPWGATEAHNLHLPYGTDTML
                     AEKVASEAAAIANQSNADAIVLPSIAYGVNSGQMDIKLCMNMRPSTQMAILDDILTDL
                     NKHSINKLVIINAHGGNSFVPIIREMSLKYPKIMVCNINWWLVCKPENYFEEPGDHAG
                     ELETSVMQYFYPDLVLPLDEAGKGTENQFTVEGLREKWAWTPRKWIYATTDTGVGNPS
                     KSTPDKGKKFFNDCVNKIAKFIIEFSSVNSEKDLYEERK"
     gene            2070..>2537
                     /locus_tag="P5293_08590"
     CDS             2070..>2537
                     /locus_tag="P5293_08590"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRR49989.1"
                     /translation="MVSYKELGLVNSRELFAKAIKGGYAIPAFNFNNMEQMQAIISAC
                     VETKSPVILQVSSGARKYANQTILRYMAQGAVEYAKELGCAIPIVLHLDHGDSFELCK
                     SCIETGFSSVMIDGSHLPFDKNIELTRQVVEYAHSYDVTVEGELGVLAGVEDDV"
BASE COUNT          694 a          486 c          476 g          881 t
ORIGIN      
        1 gcattcacgg agtataaggt ttaaacatcc cgtaaaaggc gatgaggttt atgttgaagc
       61 tccggtccca aacgacaatc tctggagggc acttactcaa actttgtagg ctgacatatc
      121 gttaccggac aattcggctt ctcttatttc tgatgaggat atgtctatca taggagcatc
      181 tagcggtttg actccatatt ttttacataa cgactcaata tcatatcctt ttcgtgggta
      241 aacccagact tcaaattcat tgagtaattc tttccatctg ttccattttt caataatggc
      301 aagattgtct gcccctataa tcaaaattag cttgttgcct ttttcacgtt ctgttattgt
      361 tcttaatgta tcaatcgtgt agagtggttt agggagttta aattctatag atgaaacgtg
      421 tatcttacat ttgagtcctt tttgaagagc ctctttttca agatttgcag ctactttttt
      481 tacatgtttc aatcgttctt cggcactctt ttcattggac tcatctttaa gtggattagc
      541 ggggctgacg acaagtctca attcatcaat gtcgcaattc tccattaagt agcggcagat
      601 agtcagatgc cctacatgca tgggattaaa agacccgaaa tatagggcga ttttcatgac
      661 aggaattctt cggttatctg ttcagcttct tttagacatt gttcaagaga atcatttaca
      721 agtactttgt caaactgact ttcaaatgtg agttcatatt ctgctttttt tattcttcgt
      781 tcaagtgaag cgctgtcttc acttcctctt ttgacaagtc tttcgcgcag aacttcaata
      841 gagggtggtt ttataaaaac agcaagcgca gcatctccgt aaagtttttt tagattcaat
      901 cctcctttga catctatgtc aaatactatt actttacctt cattccaaag cctttcaact
      961 tcacttttaa gtgtgccgta ataatatccc ttataaactt cttcatactc aacaaactcg
     1021 ttattggcta ttttttttcg aaactcctcg gtgctcagga aataatactc tcttccatta
     1081 acttcgtttt ctcttggctg tcgggatgtt gcagaaattg aaaatccaag caccggaaac
     1141 ttcttcatca aatgagagac aatcgtactc ttgcccgcac ccgaaggggc agaaaaaatc
     1201 atggctctat tttctttctt cataaagatc tttttctgag ttcacagagg aaaactcaat
     1261 tataaatttt gctattttat taacacaatc attgaaaaac tttttcccct tatccggagt
     1321 agacttcgaa ggattcccga cgccagtgtc agttgttgcg tagatccatt ttcgaggtgt
     1381 ccatgcccac ttttctctaa gcccttcgac tgtaaattga ttttctgttc cttttcctgc
     1441 ttcgtcaaga gggagtacaa gatcaggata gaaatattgc attacggaag tttccaactc
     1501 ccctgcatga tcacctggtt cttcgaaata gttttccggt ttgcaaacaa gccaccagtt
     1561 tatattgcaa accattattt ttggatattt caaagacatt tctctgatta tgggaacaaa
     1621 ggaattgcct ccatgtgcat tgataattac aagtttatta atactatgtt tgttaagatc
     1681 agtcaaaatg tcatctaaaa tagccatttg agtggaagga cgcatgttca tgcaaagttt
     1741 tatatccatc tggccactgt ttactccata tgctattgaa ggaagaacaa ttgcatctgc
     1801 atttgactgg ttggctattg cagcagcttc tgatgcaact ttttctgcaa gcatagtgtc
     1861 ggttccatat gggaggtgaa gattatgggc ttcagtagca ccccatggaa gaagtgcaat
     1921 tttaatattg ctattattta ttgatttcca cgtatttttc tcaagcacgg atgatttcat
     1981 gcatcaaatt gttttaaata caaagttaaa ttttattgtt aactttgagg aagtttattc
     2041 attttcaatt ttattaaaaa cattttcaaa tggtatctta caaagaactt ggcttggtaa
     2101 attcaaggga gttgtttgcc aaagcaatta aagggggata tgctattcct gcattcaatt
     2161 tcaataatat ggagcaaatg caggctatta tctccgcatg tgtcgagacg aagtctcctg
     2221 ttatactgca ggtttcaagc ggagccagaa aatacgccaa tcagacaatt ctgaggtata
     2281 tggctcaggg agcggttgag tatgccaaag agttgggatg tgccatacca attgttttac
     2341 atctggatca tggtgattct tttgaacttt gcaaatcatg tatcgagaca ggattttctt
     2401 cagtaatgat tgatggttca catcttcctt ttgataagaa tattgaactt accagacagg
     2461 ttgtagagta tgctcattcg tatgatgtta ctgttgaggg tgagcttgga gttcttgccg
     2521 gagttgagga cgatgtt
//
LOCUS       DAOTQL010000200         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_32510_length_2510_cov_5.026069, whole genome shotgun sequence.
ACCESSION   DAOTQL010000200 DAOTQL010000000
VERSION     DAOTQL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32510_length_2510_cov_5.026069"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="rsgA"
                     /locus_tag="P5293_08595"
     CDS             complement(<1..858)
                     /gene="rsgA"
                     /locus_tag="P5293_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRR49990.1"
                     /translation="MDKETIKQARVVKHTGSHYLVSDLPEWKLTKCIVRGKLRLTDSR
                     TTNPIAVGDMVDYELQSDGTGIITKIYPRHNYIIRKSANLSKESHIIAANIDMAYLIV
                     TLICPKTPLPFIDRFLVTCEAYSVPVKIILNKCDLYEEIKESVESFKDLYKSAGYTTI
                     EVSCTTGQNIEILKEECKGKITLFAGISGVGKSTLINTLDSSLNLKTREISEYHLQGK
                     HTTTFYEILPLRSGGFVIDSPGIKGFGLIEMKPEELSHYFPEMLRVLDNCRFAPCTHT
                     HEPDCAVKKA"
     gene            897..1901
                     /locus_tag="P5293_08600"
     CDS             897..1901
                     /locus_tag="P5293_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRR49991.1"
                     /translation="MKNIQNKIVMYTLEELDSIVERALMNINLKGKPEELYSPIEYLI
                     SIGGKRLRPKLALMTYNLFSDDINNEILYPALAMEIFHGFTLIHDDIMDDASMRRNQM
                     TVHKKWNNNIAILSGDVMCIKSYQYIAHAPEQVLSKVLELFSKTAAQVCEGQQLDLNY
                     ESVPFITMDDYTEMISLKTAVLIACSAKIGAIIAKAEDKITKFLYDYAFKLGLAFQIK
                     DDYLDSYGDASTFGKAIGGDILSNKKTWLLVEAFRRANSEQTNTLLALFRDENISAKE
                     KIIKVLSVYDEIGVREHAEKAIARYHAEARDIVLSAGLTCAQEDQLISFSERLINREK
                     "
     gene            1910..>2510
                     /locus_tag="P5293_08605"
     CDS             1910..>2510
                     /locus_tag="P5293_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HRR49992.1"
                     /translation="MKYSDKFNEIFDRSVNDYHKWDNVDAEMINPFDLGTLEYFLYKK
                     NWIDAVQWHLEDIIRDPGIDPVKALTIKRRIDNSNQERTDLVEMIDSYFMELFSNVIP
                     RNDATINTETPAWAIDRLSILALKIYHMKIESERNDASSDHIARCKEKYRTLLTQRED
                     LSLAIDQLLTDIEEGRKFMKFYKQMKMYNDPALNPILYQS"
BASE COUNT          802 a          423 c          497 g          788 t
ORIGIN      
        1 agcttttttg acggcacaat caggctcgtg agtgtgagtg cagggagcaa atctgcaatt
       61 gtctaaaact cggagcattt caggaaaata gtgactaagt tcttctggtt tcatttcaat
      121 cagaccaaat cccttgatac ctggagaatc aataacaaaa cccccacttc tcagaggtag
      181 tatttcataa aatgttgtag tgtgttttcc ctgaagatga tattctgaaa tctctcttgt
      241 tttgagatta agtgagctgt caagtgtatt gataagagtg gacttcccaa ctcctgaaat
      301 tcctgcaaac agagttattt ttcctttgca ctcttcttta agtatttcaa tattctggcc
      361 agttgtacag gatacttcaa ttgtagtgta gccggcagat ttatagagat ctttaaagga
      421 ttctacactc tcttttattt cttcgtacag gtcacacttg ttaagaataa tcttgacagg
      481 cacgctatat gcttcacaag ttaccagaaa tctgtcaata aagggaagag gagttttagg
      541 gcatataaga gtaacaatta gatatgccat atcaatattt gcagcaataa tatgggattc
      601 tttggaaaga ttggcagatt tgcgaataat gtaattatgc cttgggtaaa tttttgtaat
      661 tatgcctgtt ccatcactct gcaattcata atcaaccatg tctcctactg caatggggtt
      721 tgttgttctg ctatctgtca gtcggagttt gccacgtaca atacatttag taagcttcca
      781 ttctggcaag tcgcttacaa gataatgact tccagtgtgt tttactactc ttgcctgctt
      841 tattgtttct ttgtccacag ctgcgaatat acaaaattgt ctaaaaaaga ttaactttga
      901 aaaatattca gaacaaaata gttatgtata cgctggaaga gcttgattcc attgttgaga
      961 gggctttgat gaatataaat ctcaaaggaa agcccgaaga actttatagc cctatagaat
     1021 atctaatttc tataggtggt aaaagattga gaccgaaact cgctttgatg acctataatt
     1081 tattttcgga tgatatcaat aatgaaattt tatatccggc tcttgcgatg gagatattcc
     1141 atggatttac tcttattcat gatgacatta tggatgatgc ctccatgcgg aggaatcaaa
     1201 tgaccgttca caaaaaatgg aataacaata ttgcgattct ttctggtgac gtgatgtgta
     1261 taaaatctta tcaatatatt gcacatgctc cagagcaggt tttaagcaaa gtcctggaac
     1321 ttttttcaaa aacagctgct caggtttgtg aaggtcagca gttagatttg aattacgaat
     1381 ctgttccttt tattactatg gatgattaca ctgaaatgat atcgcttaaa acagctgtat
     1441 taattgcttg ttctgccaaa ataggagcaa ttattgccaa agccgaagat aagattacaa
     1501 aatttttgta tgattatgcc tttaaacttg gtctggcatt tcagataaag gatgactatc
     1561 ttgacagtta tggagatgct tcaactttcg gcaaagcaat aggaggtgat atattatcta
     1621 acaaaaaaac atggttgctt gtcgaagcct tcagacgtgc aaattccgag caaacaaata
     1681 ctctacttgc gctattcaga gatgaaaata tttctgctaa agaaaaaata attaaagttt
     1741 tgtctgttta cgatgagata ggtgtaaggg aacatgctga aaaagcgatt gcaagatatc
     1801 atgctgaagc gagagatata gtgctttcag ctggcttgac atgtgctcag gaggaccagc
     1861 tgatttcctt tagcgaacgg ttgattaaca gagagaaata aatcagggaa tgaaatacag
     1921 cgataagttt aatgaaattt ttgatagatc tgtaaatgat tatcacaaat gggacaatgt
     1981 tgatgcggag atgattaatc catttgatct cgggactctt gaatatttct tatacaaaaa
     2041 aaactggata gatgctgttc aatggcatct cgaagatatt atcagagacc ctgggataga
     2101 tccggtcaaa gctcttacta tcaaacggag gattgacaat tccaatcagg agagaacaga
     2161 tcttgttgag atgatagaca gttatttcat ggaacttttt tccaatgtta ttcctcggaa
     2221 tgatgccaca atcaatacag agactcctgc ttgggctatt gacagactct caatacttgc
     2281 tcttaagata taccacatga agattgagag tgaaaggaac gatgcttcaa gtgatcatat
     2341 tgccaggtgt aaagaaaaat atcgcacact tcttactcaa agagaagacc tttcgcttgc
     2401 aatagaccag cttttaactg atatcgaaga ggggagaaaa ttcatgaagt tctataaaca
     2461 gatgaagatg tataatgacc ccgcattaaa tccgatactt tatcagagtg
//
LOCUS       DAOTQL010000201         2496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_32772_length_2496_cov_3.181483, whole genome shotgun sequence.
ACCESSION   DAOTQL010000201 DAOTQL010000000
VERSION     DAOTQL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32772_length_2496_cov_3.181483"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="P5293_08610"
     CDS             <1..136
                     /locus_tag="P5293_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRR49993.1"
                     /translation="YLSKGVLAKSNGELVSKVVRLAGELGREIASPSEARKILGLKEK
                     "
     gene            169..1209
                     /locus_tag="P5293_08615"
     CDS             169..1209
                     /locus_tag="P5293_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRR49994.1"
                     /translation="MKKGNKYGIHRVIEPKGVLPQPANKLDNNMDEIYDNEILIDVKT
                     LNIDSASFTQIEEQAGGDKAKIAEIMMDIVAKQGKHRNPVTGSGGMLLGVVEKIGDAL
                     IGKTDLKVGDKIATLVSLSLTPLRIDKIKEIRSDVDQVDIDGKAILFESGIYAKIPDD
                     MPEKLALSALDVAGAPAQTAKLVKPGDTVLIIGAGGKSGMLCCYEAKKRAGVTGKVIG
                     MCHSEKSTQRLKDLDLCDYVFSGDATQPVAILEKIEEITKGEMCDITINNVNITDTEM
                     TSILCTKDKGIVYFFSMATSFTKAALGAEGVGSDVTMIVGNGYTKGHAAITLQILRES
                     EKLRKVFTELYA"
     gene            1218..2252
                     /locus_tag="P5293_08620"
     CDS             1218..2252
                     /locus_tag="P5293_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRR49995.1"
                     /translation="MTKACRYGTHRVISPAGTLPQPAYKLDNTMRIYENEVLIDVKTL
                     NIDSASYTQIKNACNSDAEKMKQMILSIVEERGKLQNPVTGSGGMLIGTVKEIGNEFP
                     ANRLKMGDRIATLVSLSLTPLKIYSIEKLSPDSDQVDVKAEAILFESGLYAILPDDIP
                     EKLALAALDVAGAPAQVDRLVTEGDIVCIIGGGGKSGILCCYQAMQNVGPYGKVIVVE
                     HSKENAQRIIDMNLATDVIIADATNVMNVYNKVMAVTGGRGCDVTVNNVNVPSTEMTS
                     ILVTKGQGYVYFFSMATSFTKAALGAEGVGKDINLIVGNGYAKGHANLTLNILRESKP
                     IRELFEKIYT"
     gene            2255..>2496
                     /locus_tag="P5293_08625"
     CDS             2255..>2496
                     /locus_tag="P5293_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRR49996.1"
                     /translation="MAVDRRKILFPEVSDEQWNDWKWQVKNRIETLEDLKKYIKLTAE
                     EEEGVKKSLETLRMAITPYYLSLIDPDNPDCPIRKQA"
BASE COUNT          841 a          381 c          554 g          720 t
ORIGIN      
        1 ttatttgtcc aagggagttc ttgcaaaatc aaatggggag ctggtatcaa aagttgtaag
       61 gttggctggc gaactgggtc gagaaattgc atctccttct gaagcacgta aaattttagg
      121 tttaaaagaa aagtaaaaaa ttaacaacaa tttaaattat aaataaatat gaaaaaaggt
      181 aataaatatg ggattcacag agttatagaa cctaaaggtg ttcttcctca accagccaac
      241 aaacttgaca acaatatgga tgaaatctat gataatgaaa tacttatcga tgttaaaact
      301 ttaaacatag attctgccag ttttacacag attgaagagc aagctggagg tgacaaggct
      361 aaaatagctg aaataatgat ggatatagtt gcaaagcagg gaaagcacag aaatcctgtg
      421 acaggatcag gaggcatgtt gctgggagtt gtagagaaaa taggagatgc acttattggc
      481 aaaacagatt tgaaagttgg cgataaaatt gcgacacttg tttctctctc tcttacacct
      541 ttaagaattg ataaaattaa ggagattcgt tcagatgtcg accaggttga tattgatggt
      601 aaggcaattt tgttcgaaag cggaatatat gctaaaattc ctgatgatat gcctgaaaaa
      661 cttgctcttt ctgctcttga tgttgccgga gctccggcac agacagcaaa attggttaag
      721 cctggtgaca cagttttgat tataggcgcc ggcggaaagt ccggtatgct atgttgctat
      781 gaggcaaaaa agcgggcagg agttacagga aaagtgatag gcatgtgtca tagtgaaaag
      841 agcactcaga gacttaagga tttggattta tgtgattatg ttttttcagg cgatgcaaca
      901 caaccggtcg ctattcttga aaaaattgaa gagattacaa aaggagaaat gtgtgatatt
      961 actataaata atgtaaatat tactgatact gagatgacct ctattctttg tacaaaagat
     1021 aaaggtattg tttatttctt ctctatggct acaagcttca caaaggcagc tctgggagca
     1081 gaaggagttg gcagtgacgt taccatgatt gttggaaacg gatacaccaa aggacatgct
     1141 gcaataactc ttcaaatatt aagagaaagt gagaagctta gaaaagtgtt tacagaactt
     1201 tatgcatagt tttttttatg acaaaagctt gcagatatgg aactcataga gtgatttccc
     1261 ctgccggtac tcttcctcaa ccggcctata agctggataa taccatgaga atatatgaga
     1321 atgaggtatt gattgatgtt aagactctta acattgattc tgcatcttat actcagataa
     1381 aaaatgcttg taattctgat gcagaaaaga tgaaacaaat gatattatca attgttgaag
     1441 aaaggggaaa attacaaaat cctgttactg gttcaggagg gatgttaatc ggaacagtca
     1501 aggagatagg aaatgaattt cctgcaaaca ggcttaaaat gggagacagg attgcaactc
     1561 ttgtctctct ctctttgacc cctttgaaga tttattcaat tgaaaaacta tctcctgatt
     1621 cggatcaagt tgatgttaaa gctgaagcta ttctttttga aagtggactt tatgcaatat
     1681 tacctgacga tattccagag aaacttgctc ttgcagcact cgatgtagca ggagcacctg
     1741 cccaggtgga cagactcgtt acagaaggag atatcgtatg tattattggt ggaggcggca
     1801 agtcgggtat tctttgctgt tatcaggcaa tgcaaaatgt aggaccttac ggtaaagtta
     1861 ttgtcgttga acattcaaaa gagaatgccc aaagaattat tgacatgaat cttgcgactg
     1921 atgtgataat tgctgatgcc accaatgtta tgaatgttta caataaagtc atggctgtta
     1981 ccggaggcag gggatgcgat gttactgtaa ataatgtcaa tgtaccttct actgagatga
     2041 cttcaattct tgtaacaaaa gggcagggat atgtttactt tttttcaatg gcgacaagtt
     2101 ttacaaaagc tgcattggga gctgaaggag tgggaaaaga catcaatctt attgttggca
     2161 atggttatgc aaaaggacac gccaatctta ctctcaatat tctgagagaa tccaaaccaa
     2221 tacgtgaatt atttgaaaaa atttatacct aattatggca gttgatagaa gaaaaatttt
     2281 atttccggaa gtttctgatg aacagtggaa tgactggaaa tggcaagtta agaacagaat
     2341 tgagactctt gaagatctta aaaaatatat taagttgaca gcagaagagg aggaaggtgt
     2401 taagaagtca cttgagactc ttagaatggc tattactcct tattatcttt cactgattga
     2461 tcctgacaat cctgattgtc cgatcagaaa gcaggc
//
LOCUS       DAOTQL010000202         2492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_32835_length_2492_cov_4.637259, whole genome shotgun sequence.
ACCESSION   DAOTQL010000202 DAOTQL010000000
VERSION     DAOTQL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32835_length_2492_cov_4.637259"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="P5293_08630"
     CDS             complement(<1..1294)
                     /locus_tag="P5293_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRR49997.1"
                     /translation="MYNDLVSKKAKLALVGLGYVGLPIALEFAKKISVIGFDINEERL
                     DKMRQGIDPCNELGPEAFEGCDIVYTSSLEVLGKASFYIVAVPTPIDDANMPDLRPLL
                     SATRSVGTVLEKGNYVVYESTVYPGCTEEDCVPLLEELSGLKCGVDFKVGYSPERINP
                     GDAVHTISSTLKIVSGCDKEALETIASVYELVVKPGVHKAPSIKVAEAGKIIENTQRD
                     VNIALMNELSILFGRMGINTYDVLEAAGTKWNFLKFSPGLVGGHCIGVDPYYLVYKSH
                     KLKYHPQVIDAGRFVNDSMGGYIGKKVVKSLIHLGRNVMASKVLVMGITFKEDVSDIR
                     NSKVVDIIRELQDYGASVDIVDPLADGAEVRAEYGLELVSSPQPQSYDAVIVAVPHKK
                     YRGLTEEYFQSLTTSPNALLADIKGLYRGQITHMTYWSL"
     gene            complement(1359..2393)
                     /locus_tag="P5293_08635"
     CDS             complement(1359..2393)
                     /locus_tag="P5293_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR49998.1"
                     /translation="MTKKTALQINELRQTPTGGLRGSGSLLITGGAGFIGSNLCEFFL
                     NSGYEVTCLDNLSTGFKSNMDEFIDHPAFKFVEGDIRDIDVCRQVTKGVDYVLHEAAL
                     GSVPRSINDPITSNAVNVGGFLNMLVAARDAGVKRFIYAASSSTYGDSQELPKVEEKI
                     GKPLSPYAVTKYVNELYADVFSKTYGISCIGLRYFNVFGRRQSPEGAYAAVIPLFVKD
                     LIKHQRPVINGDGEYSRDFTYIDNVILANYLAVTTPNTDALNQVYNVAFGERTTLNQL
                     FEYIRNGLSKFDPTIAEIVPVYGPFRAGDIPHSLASVEKARRLLGYEPQFSVKSGLEK
                     ALQWYWENLQ"
BASE COUNT          773 a          633 c          434 g          652 t
ORIGIN      
        1 acaggctcca ataggtcatg tgagtaattt ggcctcggta aagacccttg atatcggcaa
       61 gaagagcgtt aggtgaggtg gtgagtgact gaaaatactc ttcggtaagg ccgcgatatt
      121 ttttgtgagg aacggccaca atcacagcat catagctttg aggctgaggt gaggatacca
      181 actcaagccc atactcggca cgcacctcag ccccatccgc cagcgggtcc acaatatcaa
      241 cacttgcccc ataatcctgg agctccctga taatatcgac aacttttgaa ttgcgaatat
      301 cagaaacatc ttccttgaaa gtaattccca tcacgagcac cttggaagcc atcacattac
      361 gcccaagatg aatcagactt ttaacaactt tctttccaat ataaccaccc atagagtcat
      421 tcacaaaacg tcccgcgtca ataacctgtg gatgatattt caacttatgg gacttatata
      481 caaggtaata tgggtcaaca ccaatgcagt gaccaccaac cagaccaggc gagaacttca
      541 gaaaattcca cttagtccca gcggcctcaa gcacgtcata agtattaatt cccatccttc
      601 caaaaagaat cgaaagctca ttcatcaacg caatattcac gtcacgctga gtattctcaa
      661 taatcttgcc cgcctcagcc accttaatac ttggtgcctt gtgcacaccc ggtttcacaa
      721 ccaactcata tacagaggca atagtctcaa gagcctcctt atcgcaacct gacacaattt
      781 tcaaagtact ggaaatagta tgaacagcat cgcctggatt tatcctttca ggtgagtacc
      841 cgactttaaa atcaacccca cacttcagtc ctgacagttc ctccagaaga ggtacacagt
      901 cttcctcagt acaaccaggg tagacagtag actcgtaaac aacataattt cctttttcca
      961 acacagttcc aacacttctt gtagcactaa gcaacggtct caaatccggc atgttagcgt
     1021 cgtcaatcgg agtaggcacg gcaactatat aaaatgaagc cttaccaaga acttccaaag
     1081 aagaagtgta aacaatgtca cacccctcaa atgcttctgg gccaagttca ttgcaaggat
     1141 caataccttg tcgcatctta tccagccgct cctcatttat atcaaatcca ataacagaaa
     1201 ttttctttgc aaattcaagt gcaatcggca aaccaacata tcccagccct accagagcga
     1261 gctttgcctt tttggaaacg agatcgttat acatatttta tattttaatt aacatttaca
     1321 aatcaccaaa aatcgtcaca aatcaccaaa aaattatttt attgcaaatt ttcccaatac
     1381 cattgcaatg ctttttcaag tccgctttta actgaaaatt gaggctcata cccaagtaac
     1441 ctccttgcct tctcaacaga tgccagagaa tgaggaatat caccagccct gaacggacca
     1501 tatacaggga caatttccgc aatagtcgga tcaaatttgg aaagtccgtt tcttatatat
     1561 tcaaacagct gattcaaagt ggtccgttct ccaaaagcaa cattgtaaac ctgattaagc
     1621 gcgtcagtat tgggagttgt aactgcaaga taattagcca aaatcacatt gtcaatatac
     1681 gtaaaatctc tcgaatattc tccgtctcca ttgatcaccg gcctctgatg tttaattaaa
     1741 tctttgacaa acagaggaat aacagctgca taagcaccct caggactctg ccgccggcca
     1801 aaaacattaa aatatctcaa ccctatacaa ctaatcccat aagttttaga aaacacatca
     1861 gcgtaaagct cattcacata tttggttaca gcataaggcg ataacggctt gccaatcttc
     1921 tcctcgacct tcggcagctc ttgcgaatct ccataagttg acgatgacgc tgcataaata
     1981 aacctcttga caccggcgtc cctggccgca accaacatat tcagaaaccc gcccacgtta
     2041 acagcgttag aagttatggg atcattaatc gagcgcggta cagagccaag tgcagcctca
     2101 tgaagcacat agtcaacacc tttggtaacc tgcctgcaca catcaatgtc gcggatatca
     2161 ccctcaacaa atttaaaagc agggtggtca ataaactcat ccatgttcga tttgaatcct
     2221 gtagaaagat tgtcgaggca agtcacctcg tagcctgaat ttagaaaaaa ctcacaaaga
     2281 ttagagccga taaaaccggc tcctccagtg atgagcaaag acccactccc gcgcagacca
     2341 cctgtcggag tttgtcgtaa ttcgttgatt tgtagagctg ttttttttgt cataaagttg
     2401 ttactttttt tggttataac cgtaaaaatt tcctcgccca tctgcgtatt tcaatctgcg
     2461 aagatctgcg agagatgtat ttttttcttt ct
//
LOCUS       DAOTQL010000203         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_33354_length_2464_cov_2.846409, whole genome shotgun sequence.
ACCESSION   DAOTQL010000203 DAOTQL010000000
VERSION     DAOTQL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33354_length_2464_cov_2.846409"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="P5293_08640"
     CDS             <1..1324
                     /locus_tag="P5293_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HRR49999.1"
                     /translation="ADPYPVYPIGISYLQSYLSKHLPELNITSVDMNLIDMDGLAILL
                     NREHFDYIGVSLRNIDGANSLDRTSFISGYNEIIDCIKSNSDAVLILGGAGFSIFPEA
                     LMSILEPDYGIIGEGEESLRLLLQKLDCGGDVLDIEGIVKKTKDGFVRKEHSEYLRSL
                     ELCFDDTLAYYYWEHSGMLNIQTKRGCCYNCIYCSYPLIDGRKVRTLDPDLIVETLVR
                     LEKDKGINYVFFTDSVFNIQNDYNVELAEKIIRSGVKINWGAYFSPHNLTDELLEIFR
                     RSGLKHIEFGTESLSSSQMERYGKNFSFEDVLLNSERCLKHNIFYAHFLILGGLGETH
                     ETLKETMENSKKIRFSVFFPYIGMRIYPGTPLQKIAIEMGKIDVNDTLLEPTYYIEEG
                     FDLEECRKMALDTNKAWVFPDDPLFGQLEYMRSVKKKKGLIWEYLRKP"
     gene            1300..1749
                     /locus_tag="P5293_08645"
     CDS             1300..1749
                     /locus_tag="P5293_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HRR50000.1"
                     /translation="MGIFEKALISGEEILTFIPQRFPIVMVDSYYGKDGNVSYTGYTP
                     AESTLFCRDGVFTEPGIIEHMAQSAALSAGVDFKCRGEEIPVGFIGALSKLKIERLPE
                     TGHMLETTVSEIQKYQDISLVSVRVESFGKVVAEGELKIFLQREEKQ"
     gene            1753..2202
                     /locus_tag="P5293_08650"
     CDS             1753..2202
                     /locus_tag="P5293_08650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRR50001.1"
                     /translation="MRKKTVTDSKELVCRSKIKVRFNEVDSLKIVWHGEYVKYMEDGR
                     EAFGLEYPGLGYLDIYNFGYTAPVVDLHISYKSPLVYGDSAIIETRYIYTQAAKIMFE
                     FRIFRESDGQLAAEGSSVQVFLDSEGELVLINPEFYTNWQNKWLKGI"
     gene            2184..>2464
                     /locus_tag="P5293_08655"
     CDS             2184..>2464
                     /locus_tag="P5293_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50002.1"
                     /translation="MAEGDLRKVYVASSSMISPLGTTVEENMRAIEHYKSAISLIDDR
                     SVFCMPLYAAKISEDLSYGDNLSILEGRAVLSVQRALEALGKEPDEEFW"
BASE COUNT          763 a          358 c          570 g          773 t
ORIGIN      
        1 ggcagatccc tatcctgttt atcctatagg tatatcctat ttgcaaagct atttatccaa
       61 acaccttccg gagctcaata ttacttctgt tgatatgaac ctgattgata tggatgggct
      121 tgctatactt ctcaatagag agcactttga ttatataggt gtatcactca gaaacattga
      181 tggagcaaat tctttggaca ggacaagttt tatatcgggt tataatgaga ttattgattg
      241 tatcaagagt aattctgatg ctgttttaat tcttggtggt gctggatttt caatatttcc
      301 tgaggctctc atgtctattc ttgagcccga ctacggaatt attggagagg gagaagaaag
      361 tctgaggtta cttctgcaga agctcgactg cggaggagat gtgcttgata tagaaggcat
      421 tgttaagaaa acaaaggatg ggtttgtcag aaaagaacat tctgaatatt taagatcact
      481 cgagctttgc tttgacgaca ctcttgctta ctattactgg gagcatagtg gaatgctgaa
      541 tatacaaaca aaaaggggat gctgctataa ttgtatttat tgttcttatc ctcttattga
      601 tggaagaaag gtaagaacac ttgatccgga tttgatcgta gagacattag tcagactgga
      661 aaaagacaaa ggtattaatt atgttttctt tactgattca gtattcaata ttcaaaatga
      721 ctataatgta gaattggctg aaaagataat caggagtgga gtgaagatta actggggagc
      781 atatttctct cctcataatc ttactgacga gttattggaa attttcagaa gatccggtct
      841 taaacacata gaatttggca cagaatctct ctcgtcatca cagatggaac gttatgggaa
      901 aaacttttca tttgaggatg tgctgttaaa ttcggagaga tgtttaaagc ataatatttt
      961 ttatgcacac tttttaattt tgggaggttt gggtgagact cacgaaacat taaaagagac
     1021 gatggaaaat tctaaaaaaa ttcgtttttc tgtatttttc ccttatattg gtatgcgaat
     1081 atatcccgga acgcctttgc aaaagatagc cattgagatg gggaagattg atgtaaatga
     1141 taccctgctt gaaccgactt actatattga agaaggattt gatctggaag agtgtagaaa
     1201 gatggcactt gacacaaata aagcgtgggt atttccggat gatcctcttt ttggacaatt
     1261 ggagtatatg agaagtgtaa aaaagaaaaa aggattgata tgggaatatt tgagaaagcc
     1321 ttaatatcag gtgaggaaat tttgacattt atacctcaga gatttccaat agtaatggta
     1381 gacagttatt atggtaaaga cggcaatgtc tcttataccg gatatacacc tgcagaaagt
     1441 actctttttt gtcgagatgg agtttttaca gagcctggta ttattgagca tatggcgcag
     1501 tctgcagcat tgtcggccgg agtggatttt aaatgcagag gcgaggagat tcccgtgggt
     1561 tttatagggg cactaagtaa acttaagatt gaaagacttc cggaaactgg ccatatgtta
     1621 gaaacaactg taagtgaaat tcagaagtat caggatatat ctcttgtatc tgtcagagtt
     1681 gaaagttttg gaaaagttgt ggcagaggga gaattaaaga ttttccttca aagggaagaa
     1741 aaacaataag gtatgaggaa aaaaacagtg acagacagca aagaattagt ttgcagatct
     1801 aaaattaagg tcagattcaa tgaggtagat tctctcaaaa ttgtatggca tggtgagtat
     1861 gtaaaataca tggaagatgg gagagaggct ttcggattgg aatatcctgg tcttgggtat
     1921 cttgatattt ataattttgg ttatactgcg cctgttgtcg atttgcatat cagctacaag
     1981 tctccgcttg tctatggtga tagtgccatt atagagacaa ggtacatcta tactcaggct
     2041 gccaaaataa tgttcgaatt tcgtattttc agagaatccg acggacagct tgctgctgaa
     2101 ggttcttctg ttcaggtctt tcttgattct gagggagaac ttgtgcttat taatcctgag
     2161 ttttacacaa actggcagaa taaatggctg aaggggattt aaggaaagta tacgtagcct
     2221 cgagctcaat gatatctcct cttggtacaa ctgtggagga gaatatgaga gcaattgagc
     2281 attacaagtc tgcaataagt ttgatagatg acagatctgt attctgtatg cctctttatg
     2341 ctgccaagat ttcagaggat ctttcttatg gtgataactt atcgattttg gaaggtaggg
     2401 ctgttttatc tgttcaaaga gcactggaag cacttggcaa agagcctgat gaagaatttt
     2461 ggga
//
LOCUS       DAOTQL010000204         2417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_34267_length_2417_cov_5.078323, whole genome shotgun sequence.
ACCESSION   DAOTQL010000204 DAOTQL010000000
VERSION     DAOTQL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34267_length_2417_cov_5.078323"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..1188
                     /locus_tag="P5293_08660"
     CDS             37..1188
                     /locus_tag="P5293_08660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRR50003.1"
                     /translation="MKIISSILITLAISSVAFGKDIKENLAATQLQQIMKENKAVGLA
                     VAVVKGGKIVYVGSFGKKNIENDVDLKKDDLFRIASISKSFTTTALMTLVDKGLVDIN
                     QDVSELIGFKVRNPKFPDIPITLKMLLSHTSSLRDAAGYFTLDVVNPEKNEKYGEAYH
                     DYAPGTAYDYCNLGFNIIGTIVERVSGKRFDDYVRNSVIRPLGLYADYNVDSLDTSKF
                     VTLYGNDDNGIWGARPEAYMSRGDEIKNNYVFGYSTPIFSPTGGIKISAQDLARYMIM
                     HMNYGKYGKVRVISEKSSRMMQTPVVKVDDFTEYCMALEKVTNLIPGEVMTGHTGSAY
                     GVYSAMFFNPDKRFGIVMMTNGCPPVYKDGFTVIQGEVIRALYNIFIAK"
     gene            1190..>2417
                     /locus_tag="P5293_08665"
     CDS             1190..>2417
                     /locus_tag="P5293_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR50004.1"
                     /translation="MKKLLIITYYWPPAGGSGVQRWLKFVKYFRRYGYEPVVYTPENP
                     EFMAHDQGLLAEIPQGIEVIKRKIFEPYNLYKIFTGRRGEAIKPGFIAESGGNSKKSF
                     KETFSLFVRSNLFVPDPKCMWIRPSVKYLTRYLKTNPVDIIVSTGPPHSMHLIARGLA
                     RRTGIKWIADFRDPWTTMYTFKYMKNTSLTKLLHKRMERSVIREADAVVVVTNFMKEE
                     FLALKPKRIKVITNGFDASDFAQINESLEDKFTITYTGLFVKDRNPSILWRILGDMVK
                     EVQGFGEDLLLRIIGHTDGSVWSDILGNSLDSNLVSMDYMPHEEVIKWQRRARILLLA
                     GGQEPEAKGILTGKFFEYLAARRPILGFGPLGGDMDIALKESEAGVLFDYNDYTGARI
                     WIENQYTLYKKGEIYIP"
BASE COUNT          756 a          397 c          540 g          724 t
ORIGIN      
        1 ttatctttgt attattcccc tctaaaaaaa caatgtatga aaataatttc gtcgatttta
       61 atcactttgg ctatctcttc agttgctttt ggaaaagata taaaagaaaa tttggcagcc
      121 actcaactgc aacaaataat gaaagaaaac aaagctgtag gtctcgccgt ggcagtcgtc
      181 aaaggtggta aaatagtata tgtcgggtct tttggtaaga aaaatattga aaacgatgtt
      241 gatcttaaga aagatgatct tttcagaata gcatcaattt ccaaatcttt taccacaact
      301 gctttgatga ctctggtgga caaagggctg gtggacatca atcaggatgt aagtgagctt
      361 atcggtttta aagtccgcaa tccaaaattc cccgatattc ctattactct taagatgctt
      421 ctttcacata cgtcaagtct tcgggatgca gcaggttatt ttacgcttga tgtagtcaat
      481 cccgaaaaga atgaaaagta cggagaggca tatcatgact atgctccagg aacagcatat
      541 gactactgta atctcggatt taacattatc ggcactattg ttgaaagagt gtccggaaag
      601 agatttgatg attatgtaag aaacagcgta ataagacctt tgggtctata tgcagattac
      661 aatgttgatt ctcttgacac ttcaaagttt gtgactttat atggcaatga tgataatggc
      721 atatggggag caaggccaga ggcgtatatg agcagaggag atgaaattaa aaacaattat
      781 gtttttggct acagtactcc aattttttct ccaacaggag gaattaaaat atctgcacag
      841 gatcttgcac gttatatgat aatgcatatg aattacggta aatatggcaa agtgagggtt
      901 atttccgaga agagttcaag aatgatgcag acgcctgtgg taaaggtgga cgattttact
      961 gagtattgta tggctcttga aaaagtcaca aacttgattc cgggggaggt tatgaccgga
     1021 cacacaggtt cggcatatgg agtctatagt gcaatgtttt ttaatcctga caagcgattc
     1081 ggaatagtaa tgatgacaaa tggttgccct cctgtctaca aggatggttt tacagttatc
     1141 caaggtgagg taataagagc tttgtacaac atatttatcg caaagtagat tgaaaaagct
     1201 gttgattatt acctactact ggcctcctgc aggaggatcg ggtgtccagc gatggttaaa
     1261 gtttgtaaaa tactttagaa gatatggtta cgaacctgta gtctatacgc cggaaaatcc
     1321 tgaatttatg gctcatgacc aagggcttct tgctgagatt cctcagggaa tagaggtgat
     1381 aaaaaggaaa atttttgagc cttataacct ctataagata tttacaggca gacgaggaga
     1441 agccattaag cccggattta ttgccgagag tggtggaaac agtaaaaaat cctttaaaga
     1501 aactttttct ctttttgtca gaagtaactt gtttgttcca gatccaaaat gtatgtggat
     1561 acgaccttct gtcaagtacc tcaccagata tttgaaaacg aatcctgttg atattattgt
     1621 cagtacggga cctcctcatt ccatgcattt gattgcaaga ggtctggctc ggaggactgg
     1681 aataaaatgg attgccgatt tccgtgatcc ctggactact atgtacacat tcaagtacat
     1741 gaagaataca tctttaacca aattgttaca taaaagaatg gagcgaagcg ttatcaggga
     1801 agcagatgca gttgtggtag tgacaaactt tatgaaggag gaatttcttg cactcaagcc
     1861 taagagaatc aaagtaatta ctaatggatt tgatgcctct gattttgcac aaataaatga
     1921 atctttagaa gataagttta ctattactta tactggtctg tttgttaaag accgaaaccc
     1981 atccattttg tggcgcatac ttggtgacat ggtaaaagaa gttcaaggat ttggagagga
     2041 ccttttgctt agaattattg gtcatactga cggctctgta tggagtgaca ttttgggtaa
     2101 ttctcttgat tcaaatcttg tcagtatgga ttatatgcct catgaagaag tgattaaatg
     2161 gcaaagacgt gctcggatac ttttgctggc aggaggacag gaacctgaag caaagggtat
     2221 tcttactggt aaattctttg aatatctggc tgcaagacgc cccattctgg gttttggccc
     2281 acttggaggc gatatggata ttgctttgaa ggagagcgag gccggtgttc tttttgacta
     2341 caatgactat acaggtgcaa ggatctggat agagaatcaa tatactctgt acaagaaagg
     2401 agaaatatat atacctt
//
LOCUS       DAOTQL010000205         2413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_34348_length_2413_cov_4.948261, whole genome shotgun sequence.
ACCESSION   DAOTQL010000205 DAOTQL010000000
VERSION     DAOTQL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34348_length_2413_cov_4.948261"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="P5293_08670"
     CDS             <1..692
                     /locus_tag="P5293_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR50005.1"
                     /translation="NGSESIFELGVYLSEADLRTSSLGYYLLRTGTLKGAMGWFMASD
                     SFLARLNQDPSDVRWGIMDYDETYSETGVKRYGSCRKYTGVDLKGDKGSTSAVNIKVI
                     RLSEIYLIAAEAAFPTDKALAASYLKEIRKRSPNLPEPTATTITMQMIKDERSKELFA
                     EGQRFFDMMRWNEQITFNDDFISPSVVITHRPQTIDRTFYKTILPIPQVEIDANPAIK
                     SQQNPGYAEAD"
     gene            809..2296
                     /gene="hutH"
                     /locus_tag="P5293_08675"
     CDS             809..2296
                     /gene="hutH"
                     /locus_tag="P5293_08675"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRR50006.1"
                     /translation="MDYFHITLNEQTLEDIEPLFSHETKIKLSDDAVKRIVRCREYLE
                     SKMEETDSPIYGITTGFGSLCNISINFDQLSQLQRNLVVSHSCGVGEEVPVDIVRLMM
                     VLKIRSLSFGYSAVRLETVERLVDMFNNGVYPVVYRQGSLGASGDLAPLANMSLPLLG
                     LGNVNFRGEKRLADEVNKMFGWQPLTLASKEGLALLNGTQFMLAYSLWNLRRAKKMVA
                     LADKIAALSLDAFDGRIEPFNRAVHVIRPHKGQIDTAANMLKLLEGSEIVLREKQHVQ
                     DPYSFRCIPQVHGAVRDALDYVEKAFTTELNSATDNPSVLPDEDLIISGGNFHGEPLA
                     IPLDLLSISLAELGNISERRTYQLIGGKRSLPSFLVAHPGLNSGFMIPQYVQASIVSE
                     NKQLATPACVDTIDSSQGQEDHVSMGANAATKCFRVVENLEKILAIELMNGAQALEFR
                     RPLRSSLILEEFVADYRKVVPFIAEDEVMYPHIHNSIKYIRQLTQ"
BASE COUNT          723 a          425 c          540 g          725 t
ORIGIN      
        1 agaatggcag cgaatcgata tttgaacttg gggtatatct gagtgaagca gacttaagaa
       61 cttcatctct tggttattat cttctccgta caggtacttt aaaaggagct atgggctggt
      121 tcatggccag tgactcattc cttgccagac tcaatcaaga cccttcagat gtgcgttggg
      181 gcattatgga ttatgatgag acatatagtg aaactggcgt taaaagatat gggtcatgcc
      241 gtaaatatac cggtgtcgat cttaagggag ataagggatc aacttcagct gtgaacatca
      301 aagtaattcg tctttccgag atatatctta ttgctgctga agctgccttc ccaaccgaca
      361 aagcacttgc tgccagctat cttaaagaga tacgcaagag atctccaaat ctgcctgagc
      421 caactgcaac tacaataaca atgcagatga tcaaggatga gagaagtaag gaactttttg
      481 cagaaggaca aaggtttttt gatatgatga gatggaatga gcaaatcact tttaatgatg
      541 attttatctc tccttctgtt gttataactc atagaccaca aacgatagac agaacattct
      601 acaaaacgat tcttcctatt cctcaggttg agatagatgc aaatccggct ataaagagcc
      661 agcaaaatcc tggttatgca gaggctgatt aataattatt gattatggat tgaaggggtg
      721 ctgttttaac agtgcccctt ttaatttgaa gtaattttat ttaaatttgc actcatttta
      781 aatattttaa ttataaaatc gtactataat ggactatttt catataactc tcaacgaaca
      841 gacccttgag gatattgaac ctctctttag tcatgaaaca aagattaagc tttctgatga
      901 tgccgtcaag cggatagtga gatgcagaga gtatcttgag agtaaaatgg aagagacaga
      961 cagtccaatt tatggtatta caacaggctt tggctctctt tgtaatatat caatcaattt
     1021 tgaccaatta tctcaattgc aaagaaatct ggttgtgtct cactcttgcg gtgtaggtga
     1081 ggaggttcct gttgatatag taagattgat gatggtgtta aaaataagat cattatcctt
     1141 tggatattct gcagtcaggc ttgaaacagt agaaagactt gtggatatgt ttaataacgg
     1201 agtatatcct gttgtttaca gacagggatc tttaggggca tctggcgatc ttgcaccttt
     1261 ggctaacatg tctttacctc ttctgggact ggggaatgtc aatttcagag gggaaaaacg
     1321 acttgctgat gaggtcaata aaatgtttgg ttggcaacct cttaccttgg cttcaaaaga
     1381 aggtttggct ctcctgaacg gtacacagtt catgcttgcc tatagtctat ggaatctaag
     1441 aagggcaaaa aaaatggttg cacttgccga taagattgca gcactttcat tggatgcgtt
     1501 tgatggaaga attgaacctt ttaatcgtgc ggtgcatgtt atcagacctc acaaggggca
     1561 aattgatact gctgccaata tgctcaagtt gttggaaggc agtgagattg ttttaagaga
     1621 aaagcagcat gttcaggatc cttattcatt tcgatgtata cctcaggtcc atggtgcagt
     1681 tagagatgca ctggactatg ttgaaaaagc ttttacgaca gagttaaata gtgccacaga
     1741 caatccttct gtgttgccgg atgaggattt gattatttcc ggtggtaatt ttcatgggga
     1801 accccttgca attccgctgg acctcctctc tatctctctt gcagaactgg gcaacatatc
     1861 tgaaaggaga acatatcaat tgattggagg taaacgatct cttccatctt ttctcgttgc
     1921 acaccctgga cttaattccg gttttatgat tccacaatac gttcaggctt caatagtaag
     1981 tgaaaacaaa cagctggcca cacctgcatg tgtagatact atcgattcgt cacagggtca
     2041 ggaagaccat gtaagtatgg gagcgaatgc ggcgaccaag tgctttagag ttgtggaaaa
     2101 ccttgagaag atacttgcaa tcgaattgat gaacggagcg caggcgcttg aatttaggag
     2161 accgttgagg agttcactca ttttggaaga atttgtggca gattacagaa aagtagtacc
     2221 ttttattgct gaagacgagg tgatgtatcc acatattcat aactcaatta aatatataag
     2281 acagcttact caatgagaaa attgttttat aatattggga aactggttca gactgaatct
     2341 ggtgaattac tccgtcgtcg tagtggagca gaaatggccg atttgaacat tctcgaaaag
     2401 gcatatatgt tga
//
LOCUS       DAOTQL010000206         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_35122_length_2376_cov_4.311935, whole genome shotgun sequence.
ACCESSION   DAOTQL010000206 DAOTQL010000000
VERSION     DAOTQL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35122_length_2376_cov_4.311935"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1463
                     /locus_tag="P5293_08680"
     CDS             243..1463
                     /locus_tag="P5293_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRR50007.1"
                     /translation="MSLLGKVNRKAHLFTSGRKGLIKKINAEIDHSAEIIWVHCASVG
                     EFEQGRPLIEQYRKEHLDKKILITFFSPSGYELRKNYSQADWVYYLPIDTKRNAVAFL
                     DAVKPTMALFIKYEYWYNYLQELKRRNIPTYVVSAIFIPGQRFFKWYGVIFRKMLKCF
                     SHIFVQDERSKTLLKSIGIENVTVSGDTRFDRVKTIVQSGITIPLIEFFKGDFLCWVA
                     GSTWPADERLIASVMADLPSHKLIIAPHEVDGKHIATLVNMFAECRVVRYSEVIGKEN
                     DLKTKLLLREAKVMIIDTVGILSSVYHYGLFAYIGGGFGTGIHNILEAATYGLPVIFG
                     PKYSRFKEAVDLVSLGGAKSITGSGELAESCRLYLEDPVIRKTNGRICLDYIDRNVGA
                     TRKVLDGIYNLNRI"
     gene            complement(1465..>2376)
                     /locus_tag="P5293_08685"
     CDS             complement(1465..>2376)
                     /locus_tag="P5293_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR50008.1"
                     /translation="PSSLATGSKSDLVTVSGFSAKGGANYKLNKKSSIYLNAAAYSRI
                     PYSSVFFAQGTNEITSGVKNERNFLSESGYRYIYQRGSLELTGYWAYWKNKTIVSNPY
                     TQQDNNDVSFMIQGLDALHYGTELTVTFAPYRWLSFEGNLSVGHWNWKNDVKANIYDQ
                     YSGLKIDEINVYSDGLPVGDAPQTQGGITSSFKLRNGIEIYLDWMYNGRLYADFEPTD
                     RINPNDRSYSLKLPDYNLVNLSASWRRSIKNAGKNSNYTLFLNINNLLDERYIERGKD
                     GADHTMATFRGYWGFGINGNIGIRVDL"
BASE COUNT          689 a          421 c          481 g          785 t
ORIGIN      
        1 agtctttcgg catcaacaaa aaactgtttc caaaatattt tagctttttc aggcctgctg
       61 aaaaccattt tcattagtag gagatatcta cctaccagct caagggcaac tttcattttt
      121 ttaattttat tggctcttaa actacaaaaa tactcttttt tgactatatt cgtgcccgat
      181 ttttaactga gaagaggttg aattttatat acaattttgt tttagagtta ttttatgcag
      241 ccatgtcgct gctgggcaag gtaaaccgca aggctcacct ctttacttcc ggaaggaaag
      301 gtctgataaa aaaaattaat gcggaaattg atcactcggc tgaaattata tgggttcact
      361 gtgcttctgt aggagaattt gaacagggaa ggcctttaat tgagcaatat agaaaggaac
      421 atcttgataa gaagatttta attacttttt tttctccatc cggttatgaa ttaagaaaga
      481 attacagtca ggcagactgg gtttattatt tgcccataga taccaaaaga aatgcagtcg
      541 cttttttgga tgctgtcaaa ccaacaatgg cactttttat caagtatgaa tattggtaca
      601 attatcttca ggaacttaag agaaggaaca ttcccacata tgttgtatct gccatcttta
      661 ttccaggaca gcgtttcttt aagtggtatg gtgtaatatt cagaaagatg cttaaatgct
      721 tttcgcatat ttttgttcaa gatgaaaggt caaagacatt actaaagagt attggaatcg
      781 aaaatgttac agtcagtggt gatactcgtt ttgacagggt taagacaatt gttcagtctg
      841 gtatcactat cccccttatc gaatttttta aaggagattt tctttgttgg gtagccggta
      901 gtacctggcc tgcagatgag agactaatag cttcagttat ggctgatttg ccgtcgcata
      961 aacttatcat tgcaccgcat gaggttgatg gaaagcatat tgcaactttg gttaacatgt
     1021 ttgcagaatg ccgagttgtc agatattctg aagtaattgg taaagagaat gatctaaaga
     1081 caaaattgtt attacgggaa gcaaaggtaa tgattattga tacagttgga atactctctt
     1141 cggtatatca ttatggtttg tttgcatata ttggtggagg ctttggtact gggattcata
     1201 atatacttga ggctgcaact tacgggttgc ctgtcatatt cggtccgaaa tattctcgtt
     1261 tcaaagaggc tgtagatctt gtcagtttgg gaggagcaaa aagcattacc ggatctgggg
     1321 agcttgctga atcatgcagg ctctatctcg aagatccggt aatacgtaaa acaaatggcc
     1381 ggatttgtct tgattacatt gatagaaatg taggtgctac aagaaaagta ctggatggaa
     1441 tttataactt aaatcgaatt taagttataa atcgactcgt atacctatat taccatttat
     1501 cccaaacccc cagtagcctc tgaatgtggc catagtatga tcggcgccgt ctttccctct
     1561 ttctatgtat ctttcgtcaa ggagattgtt gatattaaga aatagagtat agtttgaatt
     1621 tttccctgca tttttaatac ttcttctcca gctggcactt aaatttacca agttgtagtc
     1681 aggcaatttg agcgaatagg acctgtcgtt aggattaatc ctgtcagtag gttcaaaatc
     1741 tgcatataga cgaccattat acatccagtc aagatagatc tcaataccat ttcttaattt
     1801 gaaactggag gtaataccgc cttgggtttg tggtgcatct cctacaggca gaccatcact
     1861 ataaacattt atttcgtcta ttttaagtcc tgaatattgg tcataaatat tagccttaac
     1921 atcatttttc caattccaat gtccaactga aagattacct tcgaaagaga gccatctata
     1981 tggggcaaat gtgacagtca gttccgttcc atagtgcaac gcatcaagcc cctggatcat
     2041 gaaagagacg tcgttgttat cctgttgagt atacggattt gatacaatag tcttattttt
     2101 ccaatatgcc caatagccag tcagttccag agagcctctc tgatagatat atctgtatcc
     2161 tgattcggaa aggaagtttc tttcattttt cacaccggat gttatctcat tagtgccttg
     2221 ggcaaagaaa acactggagt agggtattct gctgtaggca gccgcgttga ggtaaatgga
     2281 acttttcttg ttaagtttat aatttgctcc gccttttgca ctgaatcctg atactgttac
     2341 aagatcactc ttgcttcctg ttgcaagact ggaggg
//
LOCUS       DAOTQL010000207         2362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_35406_length_2362_cov_4.452969, whole genome shotgun sequence.
ACCESSION   DAOTQL010000207 DAOTQL010000000
VERSION     DAOTQL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35406_length_2362_cov_4.452969"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="P5293_08690"
     CDS             <1..317
                     /locus_tag="P5293_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding oxidoreductase"
                     /protein_id="HRR50009.1"
                     /translation="ESGEKYDQAVAIGPMIMMKFVTLTARPFNLPLIVSLNTLMVDGT
                     GMCGACRVSVGGKTKFACVDGPEFDGYKVDFDEAMRRQMMYKNIEIEADHKCKIGLNN
                     EK"
     gene            307..1692
                     /gene="gltA"
                     /locus_tag="P5293_08695"
     CDS             307..1692
                     /gene="gltA"
                     /locus_tag="P5293_08695"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRR50010.1"
                     /translation="MRNKIPRVPVREQDPAVRIKNFDEVCYGYNEEEARLEASRCLGC
                     KKPSCIGQCPVSIQIPQFILEVENGNFEKAAAIIAKDSSLPAVCGRVCPQETQCEGAC
                     ILGVKGEPVAIGKLERFVADWAREKGICFSSSANLNGKKVAVIGSGPAGLACSADLAK
                     LGYQVTIFEALHEPGGVLQYGIPEFRLPKEKVVKAEVDNVRALGVNIETNVIVGRTVT
                     IDSLMNEEGFDAVFIGSGAGLPKFMGIPGENLNGVVSSNEFLTRNNLMRAYDPSYDTP
                     IYVGKKVAVVGGGNVAMDAARTALRLGADVTIVYRRTEKELPARVEEVHHAKEEGIVF
                     KMLTNPVAVLGNDKGWVTGIRCIRMELGEPDESGRRSPIPIPDSEFEMDVDVVIMALG
                     TSPNPLIANTTPGLETNKWKCIVADENGVTTKPGVFAGGDIVTGAATVILAMGAGRKA
                     AAAIDRYLQSK"
     gene            1711..>2362
                     /locus_tag="P5293_08700"
     CDS             1711..>2362
                     /locus_tag="P5293_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRR50011.1"
                     /translation="MAQKPSIPKGTRDFTPVEMARRDYIFSTIKSVFKLYGYSPIETP
                     AIENLSTLLGKYGDEGDKLLFKILNSGNAFDNIEPTLLKDSSALSLKVCEKGLRYDLT
                     VPFARFVVQHQNELQFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVVGSNSLLNELE
                     LGQIVDEVFTRFGINVIIKINNRKILSGLAEVAGCPEKLTDITIAIDKIDKVGLEEV"
BASE COUNT          732 a          350 c          580 g          700 t
ORIGIN      
        1 tggaatcagg agaaaaatat gatcaggcag tagcaatagg acctatgatt atgatgaaat
       61 ttgtaacctt aacagctcga ccttttaatt tgccattgat tgtaagtctt aatacgctga
      121 tggttgatgg tacaggaatg tgtggtgcat gcagggtttc tgtaggaggt aaaacaaaat
      181 ttgcatgtgt tgatggacca gagtttgatg gctacaaggt tgattttgat gaagccatga
      241 gacgtcaaat gatgtataaa aatattgaaa ttgaggctga tcataaatgt aaaattggat
      301 tgaacaatga gaaataagat accaagagtt cctgttagag agcaggatcc tgctgtaaga
      361 attaaaaact ttgacgaagt atgttatggt tataacgagg aagaagccag gctcgaagct
      421 tcaagatgct tgggttgtaa aaagccttcc tgtatagggc agtgtcctgt gtcaattcaa
      481 atacctcaat ttattcttga ggttgaaaat ggaaattttg aaaaagcagc tgctataatt
      541 gcaaaggata gttctcttcc tgcagtttgt ggtagggttt gccctcagga gacccaatgt
      601 gaaggggctt gtattttagg tgttaaagga gagcctgtag ctatcggcaa gcttgaaaga
      661 tttgtagctg attgggcaag agagaagggt atttgttttt caagttctgc aaatttaaat
      721 ggtaaaaaag ttgcagttat tggaagtggt cctgcaggat tagcctgttc tgccgatctt
      781 gccaagttgg gctatcaagt gactattttt gaggcacttc atgagccggg tggagtgctg
      841 cagtatggaa ttccagaatt tcgtctacca aaagaaaaag tggttaaggc tgaggtagat
      901 aatgttcgtg ctcttggagt aaatattgag acaaatgtta tcgttggtcg aactgtaacg
      961 attgattctt tgatgaatga agagggcttt gatgctgttt ttattggttc aggtgcagga
     1021 ttgcctaaat ttatgggtat tccgggagaa aatcttaacg gagtagtttc ttcaaatgaa
     1081 tttcttacca gaaataacct tatgagggct tatgatccaa gctacgatac gcctatttac
     1141 gttgggaaaa aagttgctgt tgtaggtgga gggaatgtag ctatggatgc tgcaaggaca
     1201 gctttaagac taggcgcgga tgtgacaatc gtttacagaa gaactgaaaa agaacttccg
     1261 gccagggtag aagaggttca tcatgcaaag gaagaaggca ttgtttttaa gatgcttaca
     1321 aatcctgtag cagttttagg taatgataaa ggatgggtaa cagggataag atgtatcaga
     1381 atggaacttg gggaacctga cgaaagtggg agacgttcgc ctattcctat tccggattca
     1441 gagtttgaaa tggatgttga tgtagtgata atggctctcg gtacatctcc caatccgctt
     1501 attgcaaata caactcctgg tttggaaaca aacaaatgga aatgtattgt tgccgatgaa
     1561 aatggagtga ccactaaacc tggagtattt gcaggaggag acatcgtcac aggagctgca
     1621 accgttattt tggcgatggg agctggaagg aaggctgcag ctgcaattga cagatatctg
     1681 caatctaaat aataagtttt aatatttttt atggcacaga aaccatcaat tcctaaagga
     1741 accagagatt ttacacctgt tgaaatggct cgcagggact atattttttc caccattaaa
     1801 agtgttttta aactttatgg ttattctcca attgagactc ctgcaattga aaatctttca
     1861 acacttttgg gtaaatatgg tgatgaggga gacaagttgc tgtttaaaat tcttaattca
     1921 ggcaatgctt ttgataacat tgaaccaaca cttttgaaag atagcagtgc gttgtcattg
     1981 aaagtttgtg aaaaaggtct tcgctatgat cttactgtac catttgccag atttgttgta
     2041 cagcatcaaa atgagcttca gtttccattc aaaagatatc agattcagcc tgtctggcgt
     2101 gctgacagac cacaaaaggg acgctacaga gagttttacc agtgtgatgt agatgtagtt
     2161 ggaagcaatt cactgttgaa cgagttagag ttaggacaga ttgtagatga agtgtttaca
     2221 agattcggaa ttaatgtcat tatcaaaata aacaatcgaa aaattctttc cggattggct
     2281 gaagtagcag gttgtccgga aaaacttacc gatattacaa ttgcaatcga caagattgat
     2341 aaagtaggac ttgaagaggt ca
//
LOCUS       DAOTQL010000208         2349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_35682_length_2349_cov_4.126853, whole genome shotgun sequence.
ACCESSION   DAOTQL010000208 DAOTQL010000000
VERSION     DAOTQL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35682_length_2349_cov_4.126853"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="P5293_08705"
     CDS             <1..1169
                     /locus_tag="P5293_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR50012.1"
                     /translation="YTTENAPRSLWEQPYKVIRLANNILAVIDNIKVTDAQKATLNDL
                     KGQALFFRALAHFDITRVYGYPYAKDNGASWGAAIITQSNGPEVKPPRNTVAECYNNL
                     IIPDLIQASNLMIGTKNNTTGRVNKWGAKLLLSRVYLYKGDNINALKEAEECIAGAEA
                     ASYSLWTNSGYLTGWTTKFSSESLFELVNLITDRTGNEGIGYLCWDRGYDDIILTRSF
                     WDLLSSDPNDVRVKLTTKGTKNPSSKSYNVYILKYADDPDVQNADVMILRLSEAYLNA
                     AEAAVKLNNNDKAIRYLNPIVTRANPTKSVSGTVTLDRVLLERRLELFGEGHRAFDLL
                     RNNKTIERIGNSHSKVLPDYAKSLDWNNFRIVLPIPKYETDANPSIKAQQNPEW"
     gene            1170..2234
                     /locus_tag="P5293_08710"
     CDS             1170..2234
                     /locus_tag="P5293_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRR50013.1"
                     /translation="MKKKLFFLLFFLGNILSLYGTENKPLTDGPYVFYKDNLVQVYNF
                     DVSGQISKNTFFLNSINKLKVSSEDNKYSFYVQLHPNKRESSKIIAPEKIFAISDPHG
                     NLTAFIKILQQGGVIDNSLSWKFGKNHLAILGDVADRGRDVTAIYWLIYKLEQEAEIS
                     GGKVHFILGNHEVMLLQNDLRYTNRKYITNASSLSMSLPELYGHDTELGRWLRNKNTI
                     QIIGNTLFVHGGISKEFAERYKDISIVNQLVSNNIGKAKRDMNEEELFIFSMIGPVWY
                     RGLIYKENKYFPATKEDVDMILETYKVSRIVIGHTTLNDITSTYSGKVIAIDCESKQY
                     KHLSKGLLINGNNIIIVEIK"
BASE COUNT          875 a          380 c          406 g          688 t
ORIGIN      
        1 catacactac tgaaaatgca cctcgttctt tatgggaaca accatataag gttatacgtc
       61 ttgcaaataa tatacttgca gtaattgata atattaaagt tacagatgca caaaaggcta
      121 ctttaaatga tttaaaaggt caggctcttt tttttagagc acttgcacat tttgacatca
      181 caagagtcta tgggtatccc tatgcaaaag acaatggagc ttcatgggga gctgcaataa
      241 ttactcaatc caatggtcct gaagtaaaac ctccacgaaa tactgtcgcc gaatgttaca
      301 acaatttaat tatacctgat cttattcaag cttctaattt aatgataggc acaaaaaata
      361 atacaactgg acgagtaaac aaatggggtg ctaaactgtt gcttagtaga gtatatttat
      421 acaagggaga caacataaat gctctaaaag aagccgaaga atgcattgct ggagcagagg
      481 ctgcatctta ttcattatgg accaattcag gatatttaac aggatggacc acaaaattct
      541 cttctgaaag cctttttgaa cttgtaaatt taataaccga tcgtacggga aatgagggaa
      601 taggttattt atgctgggat agaggatatg acgacataat actgaccaga tctttctggg
      661 atcttctgag ttcagatcca aatgatgtga gggttaaact aacaaccaaa ggaaccaaga
      721 acccttcttc caaatcatat aatgtatata ttttgaaata tgcagacgat cctgacgttc
      781 aaaacgctga cgttatgatt ttaagactct ctgaagcata cctgaacgcc gctgaagctg
      841 cagttaaact taacaacaat gacaaggcaa taagatactt gaatccaata gtgacaagag
      901 ccaatccaac taaatcagtt tcaggcacag ttactcttga cagagtattg cttgaaagac
      961 gactcgaatt gtttggagaa ggccacagag cttttgatct tttgagaaac aataaaacga
     1021 ttgagagaat tggaaacagt cacagcaaag ttttgcccga ttatgcaaaa tcactggact
     1081 ggaacaattt cagaattgtt cttcctatac ctaaatacga aacagatgct aatccttcaa
     1141 tcaaagccca gcaaaatccg gaatggtaga tgaaaaaaaa attattcttc cttcttttct
     1201 ttcttggaaa catattatca ctttatggaa cagaaaataa gccattaaca gatggtccat
     1261 acgtctttta taaagataat cttgtccaag tgtacaactt cgatgtatcc ggacaaatat
     1321 caaaaaatac tttttttctt aattcaataa acaaacttaa agtatcgtca gaagataata
     1381 aatacagctt ttatgtacag ctacatccta acaaacgaga atcaagcaag ataatagcac
     1441 ctgaaaaaat ctttgctata tcagatccac atggaaatct aacagcattt ataaagattc
     1501 ttcaacaagg aggggttatt gataattcat tatcatggaa atttggcaaa aatcatttgg
     1561 ctattcttgg agatgtagca gatagaggtc gtgatgtaac tgcaatttat tggttaatat
     1621 acaaacttga acaagaagca gaaattagtg gaggtaaagt tcactttata ttggggaatc
     1681 acgaagttat gttgctgcaa aatgatttaa gatacaccaa taggaaatat ataactaatg
     1741 cttcatcact ctcaatgtca ttacctgaat tgtatgggca tgatacagaa cttggaagat
     1801 ggttgagaaa caaaaataca atacaaataa ttggcaatac tctttttgtt catggaggaa
     1861 tcagcaaaga gtttgcagaa agatacaaag atatatctat tgtaaatcaa cttgtttcta
     1921 acaacattgg taaagcaaaa cgcgacatga atgaagaaga actctttata ttcagtatga
     1981 taggccccgt atggtataga ggtttaattt ataaagaaaa taaatatttt cctgcaacaa
     2041 aagaagatgt tgatatgatt cttgaaacct acaaagtttc cagaatagta ataggacaca
     2101 caacactaaa tgatattaca agtacctatt cagggaaagt gatagcaata gattgtgaat
     2161 caaaacaata taaacatctt tcaaaaggtt tgcttattaa cggaaataat ataatcatag
     2221 tcgaaattaa atagtagctc agatagttat tgattaaata ataatacgtt atgttttttt
     2281 atatttgtca ggaatattta taaatggaag aggatttttt aaatcatgct ttatcacttc
     2341 aaaacctct
//
LOCUS       DAOTQL010000209         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_35734_length_2347_cov_3.702443, whole genome shotgun sequence.
ACCESSION   DAOTQL010000209 DAOTQL010000000
VERSION     DAOTQL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35734_length_2347_cov_3.702443"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="P5293_08715"
     CDS             <1..1113
                     /locus_tag="P5293_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRR50014.1"
                     /translation="VVYDFMNSFYRTKGVSPGRSGFDAALEAEDVVNNTRKILTKLFN
                     GGVDHNRLTFSYNATDSINLIINGLVENGIHVVTTLLEHNSVLRPLYMHQQAGTIDVT
                     YVPFDPLTGYVDPDDIKKAIKENTKFVIVTHCSNVLGTIQPLAEIGKICKDKGVIFVV
                     DGSQGAGAVDFDMQACNADVYCFTGHKCLMGPTGIGGSYVREGIDIKHTRAGGTGVRS
                     AYPGHLEEYPYRLEYGTLNILGVAGLYAGVKWITEQGIMTIHNREVELWDKLRRELQQ
                     IEGVTTYCATSKVNKNPVLSFNIKGFEAGDVGTMLDVDYGIAVRTGLQCAPKVHQHIG
                     TADIHGTVRMSVGAFNTEEDINAAISAVKEIAALKN"
     gene            1326..2261
                     /locus_tag="P5293_08720"
     CDS             1326..2261
                     /locus_tag="P5293_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRR50015.1"
                     /translation="MQRILVTGGAGFIGSHLCKRLLAEGNDVICLDNYFTGSKDNLLS
                     LIGDPHFEVIRHDVINPFHAEVDQIYNLACPASPIHYQYNPIKTIKTSVMGAINMLGL
                     AKRVNAKILQASTSEIYGDPTVHPQPESYWGNVNPIGIRSCYDEGKRCAETLFMDYHR
                     QNKVRIKIVRIFNTYGPNMHPCDGRVISNFIMQAIKNEDITIYGDGLQTRSFQYVDDL
                     IDGMIKVMDTDDSFIGPINIGNPDEFSIIDLAKKVIRLTGSKSRIVKRDLPMDDPRQR
                     KPDITLAKNLLNWEPKIQLDDGLERTIDYFRNKVF"
BASE COUNT          772 a          389 c          513 g          673 t
ORIGIN      
        1 gttgtctacg atttcatgaa ctctttttac cgaactaaag gggttagtcc cggaagatca
       61 ggttttgatg cagctcttga ggcagaggat gtagtaaaca acacaagaaa aatactcact
      121 aaacttttta atgggggagt agatcataac cgtctaacat tcagctataa cgctacagac
      181 tcaatcaatt tgattattaa tgggctagta gagaatggca tacatgtagt tacaacttta
      241 ctggagcata actctgtact aaggcctctt tacatgcatc aacaggctgg aaccatagat
      301 gtgacttatg ttccgtttga tccgttgaca ggatatgttg atcctgatga tattaagaag
      361 gccataaagg aaaacacaaa atttgtaatc gtaacacact gttcaaatgt acttggaaca
      421 atacagcctt tagcagaaat aggtaaaata tgtaaggaca agggagtgat atttgtcgtt
      481 gacggcagcc agggtgctgg agcagtagat ttcgatatgc aggcctgcaa tgcagatgtt
      541 tattgtttca cagggcataa atgtcttatg ggtcctactg gaataggagg ttcatatgtg
      601 cgtgaaggga ttgacataaa acacacaaga gcaggtggta ccggagtacg ttcagcttat
      661 cccggacatc ttgaggagta tccatacaga ctggagtacg gaactcttaa tattctcgga
      721 gtagctggtc tttatgcagg agttaagtgg ataacagagc agggcataat gacaattcac
      781 aacagagaag ttgaactttg ggacaaactt cgtagagaac ttcagcagat agagggcgta
      841 accacatatt gtgcaacgtc aaaagtgaac aaaaaccctg ttctttcgtt taatataaaa
      901 ggatttgaag caggagatgt aggaacaatg cttgatgtag attatggcat tgcagtcaga
      961 acaggtttac aatgtgctcc aaaggttcac cagcatattg gtactgcaga tattcatgga
     1021 acagtacgta tgagtgttgg agcctttaat actgaagagg acataaatgc agcaatctct
     1081 gccgtgaagg agattgcagc tttgaagaac tgattcacgg aaataaagcg gagctcagtt
     1141 ccgagggtgt gcagaagaaa ttttgggcac cctctttttt tttgtccaaa ttagtattta
     1201 aattatgtta cacaagtgta aaaattttta ttacatatgt gaaatttaaa aagctaatct
     1261 gtttgtaata ataattattt atatttgtaa tagacattac tttaataatt aaaataaaga
     1321 gaagcatgca aagaatttta gtgacaggtg gggctggatt tataggttct catctctgta
     1381 aaaggcttct cgcagagggc aacgatgtca tatgcctgga taattatttc acaggtagta
     1441 aggataatct tctttcactt attggggatc ctcattttga ggtaatcagg cacgatgtaa
     1501 taaacccgtt tcatgctgag gttgaccaga tttacaatct tgcatgtcct gcatctccca
     1561 ttcattatca atacaatcca attaaaacca taaagacttc tgttatggga gccataaata
     1621 tgctcggtct ggcaaaaaga gtaaatgcaa aaattcttca agcttccaca agtgagattt
     1681 acggggatcc tactgttcat ccgcaacctg aatcctattg ggggaatgta aatccaatag
     1741 gtatcagatc gtgttatgat gagggtaaaa ggtgtgcaga aactcttttt atggattatc
     1801 acagacagaa caaagtaaga ataaagattg tacggatatt caacacctat ggtccaaata
     1861 tgcatccctg tgacggaaga gtcatttcca atttcattat gcaggccatt aaaaatgaag
     1921 acattaccat atatggagat ggcttgcaga cgagaagttt tcaatatgtt gatgatttga
     1981 ttgatgggat gataaaagta atggacacag acgattcctt cattgggcca attaacattg
     2041 ggaaccctga cgagttctcg atcattgatt tggcaaaaaa agtgataagg cttacaggtt
     2101 caaaatccag gattgtaaag agagatttgc cgatggacga tccccgtcaa cgaaagcccg
     2161 atattactct ggcaaagaat ttgttaaact gggagccgaa gatacagctt gatgacggtt
     2221 tggaaaggac aattgattat ttcaggaata aagtttttta gaaatggata taaacaatca
     2281 tgattacaca atttatctct atgggatatt tacactttgt ttcactcaat gaccacaaac
     2341 aactaag
//
LOCUS       DAOTQL010000210         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_36017_length_2333_cov_6.858209, whole genome shotgun sequence.
ACCESSION   DAOTQL010000210 DAOTQL010000000
VERSION     DAOTQL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36017_length_2333_cov_6.858209"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..754)
                     /locus_tag="P5293_08725"
     CDS             complement(74..754)
                     /locus_tag="P5293_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50016.1"
                     /translation="MFKRFFILFIMIVFISCGKSDTETKRSDKTNIKSEEERLLEQQE
                     RDKARQQMFAERKEKYKDMLAENRDSGDVRGIWIINGDDIKNIINFLNIEIKPSMHYC
                     IQVSDDMQTGKIYFTVIDKVFDIKIEKISETKYKMIYNNKERKFDVVKLKGSRDPTEW
                     YLEIFYDDFHKYNPSLTLQSSDGISFYEICTSGINDWECSVEGCLQYIKQMDEISKAE
                     SDGPLGIE"
     gene            complement(754..1872)
                     /locus_tag="P5293_08730"
     CDS             complement(754..1872)
                     /locus_tag="P5293_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50017.1"
                     /translation="MDKATVNLIAHPENIGVNTVRTVDKGIDEGKDRLIDTTERIINC
                     YKSINQRDGLTWESKDERLSRLENELLFNDNVKYAEGDNIQRISLATDPNKMHQESYE
                     LKKTIGEVYDEIHKDDSKMLSTIDKYMEIQENNLEKAQEKWKLLYPEGGSETLVYDKN
                     NNTKERNQIYDELLYYSSKSQIAALKYQDQILIHDYIIETPKEQFVNETTSKLCNVET
                     YWAYGRAVDKEERSFKEFYSEELNAGRIGSVKNGKANAYYGAGGYEWSQKYGLDLDGS
                     LKNNVGTEKRPIYRPINDKDIKDYLDKNAEGVAIIWKETNTVGVPTHYSCIVKNEKGI
                     WLDYDHNMKSNGKPVDFSKVYKITQTKIKQEKVIKENN"
BASE COUNT          634 a          488 c          257 g          954 t
ORIGIN      
        1 gtcattatct gttgaggcta ttactgctcc tttgagcgtc gtcttgtctt caacagttat
       61 atctacactg ctatcactca attccaagag ggccatcact ctcagcttta gaaatttcat
      121 ccatttgttt tatatattgc aagcaacctt caactgaaca ttcccaatca tttataccgg
      181 atgtacaaat ctcataaaaa ctaatgccgt cagaagattg aagagttaat gaaggattgt
      241 atttatgaaa atcatcataa aagatttcca agtaccattc ggtaggatct ctagatcctt
      301 ttaattttac aacgtcaaac tttctttctt tgttattata aatcatttta tatttagttt
      361 cagatatttt ttctatctta atatcaaaga ccttatcaat tacagtaaaa tatatctttc
      421 ctgtttgcat atcatctgat acttgaatgc aatagtgcat tgaaggtttt atttctatat
      481 taagaaaatt tattatattt tttatatcat caccatttat tatccagata ccccttacat
      541 ctccagaatc cctattttct gctaacatgt ctttatattt ttcttttctt tcagcaaaca
      601 tctgctggcg agccttatca cgttcctgct gttctaaaag tctttcttct tctgatttta
      661 tatttgtttt atcagatcgt tttgtctctg tatctgattt accacaacta ataaaaacta
      721 tcattataaa taaaataaaa aaccttttga acattagtta ttctccttaa ttactttttc
      781 ttgttttatt tttgtctgtg ttatcttata tactttagaa aagtcaacag gcttcccatt
      841 gcttttcatg ttatgatcat aatcaagcca tattcctttt tcatttttaa ctatacaact
      901 atagtgtgta ggtactccaa ctgtatttgt ttctttccag ataatagcaa ctccttctgc
      961 attcttatct aaatagtctt ttatatcttt atcgttaatt ggccgataaa tcggtctttt
     1021 ttcagtccca acattatttt ttaaacttcc atctaaatcc aaaccatact tctgagacca
     1081 ttcgtaacca ccagcaccat aatatgcatt tgcttttcca tttttaactg atccaattct
     1141 accagcattc aactcttcac tataaaattc tttaaaagac cgttcctctt tatcaacagc
     1201 tctcccgtat gcccaatatg tttctacatt acataacttt gatgttgttt cattaacaaa
     1261 ctgttcctta ggtgtttcaa tgatataatc atgtattaaa atctggtctt gatatttcaa
     1321 tgcagcaatt tgagatttcg agctataata taataattca tcataaattt gatttctttc
     1381 tttagtattg ttatttttat catatactaa tgtttcactt cctccttcag gatataataa
     1441 tttccatttt tcttgtgctt tttctaaatt attttcttga atttccatat acttatcaat
     1501 agttgacaac attttagaat catctttatg aatttcatca tatacttcac caattgtttt
     1561 ttttaattca tatgactcct gatgcatttt attaggatct gtcgcaagag atattctttg
     1621 tatattatca ccttcagcat atttaacatt atcattaaat aataattcat tttcaagtct
     1681 ggataatctt tcatccttac tttcccaagt cagaccatct ctttgattta tgcttttgta
     1741 acaattaata attctttcag ttgtatcaat taacctatct ttaccttcat caattccttt
     1801 atccacagtt cttactgtat tcactcctat attctcagga tgtgctataa ggttcaccgt
     1861 tgccttatcc acagtaaatc ctccactcag acttacatcc tttgtgttat actgtgatat
     1921 tgacacatct ctgtttagac cttcaattcc ggcagtgccc tctgctgcgt cccttactat
     1981 tattgtccct tcacctattg tcgcaaaatt tgtctgtctc ttatctgtaa aaccgtatgt
     2041 cgcgtttaca ctgccggtta agttatccga acctataccg cttgcattga ctccgccgcc
     2101 aagattgttt gagctgtcat tatcctttat atgactatac tctaaagtac ccgtgtcaag
     2161 ctttaattta tctctatctg atgctattac tgctccttta agggtagtct tatcctctac
     2221 atatatatct actctcccac ctgtaagact ggtctgctcc gttacccatc ttttgtttcg
     2281 gaaaatttac taacttattt ttttccctat ttctttcccg ctcccattga agc
//
LOCUS       DAOTQL010000211         2317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_36369_length_2317_cov_3.343501, whole genome shotgun sequence.
ACCESSION   DAOTQL010000211 DAOTQL010000000
VERSION     DAOTQL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36369_length_2317_cov_3.343501"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="P5293_08735"
     CDS             <1..893
                     /locus_tag="P5293_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50018.1"
                     /translation="ATSMMAALQAAGFTAATGWSCTAYQDYNCVVVQQNWYTPNVTVF
                     TVTDADLKVNRTILTENYQTALSGVVTPYGYIRRNDGVDTWSAITNLEKGLLYYSVSG
                     QDLTGQAVGADSIIKESRFNANDNPLLVAAYGTYRNYMQAKMPRYPFSKGAIIDDNGK
                     GNTAALASIMYTDYDGALKPAHPAAYNAKNYGIATDGYTTGFEAGNWYLESVRLTNIL
                     MKDITYGLTGITGLNADPVNRSIYAAGGSMISLAGYPWTSTEYSSKDAWFYFGFTGRM
                     RNLNKGTVTDVRPVSAFQLR"
     gene            927..1277
                     /locus_tag="P5293_08740"
     CDS             927..1277
                     /locus_tag="P5293_08740"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRR50019.1"
                     /translation="MIEKLQIYKDSYLLAGKLYQAMPQMDKMHRHVIGTKILDCALDL
                     FKWISLANQSREKGERMRYLDSFLSTFEQLRVYLRICNDFKLLKLSTLSDIYLLTTSI
                     SKQLSGWRNATTRT"
     gene            1496..>2317
                     /locus_tag="P5293_08745"
     CDS             1496..>2317
                     /locus_tag="P5293_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HRR50020.1"
                     /translation="MVKAEDILTAYNDCRKNKRNSASTIEFECDYESNCVSLYEEINN
                     RTYHPTKSIAFIVTKPRRREIFAANFRDRVVHHLIDIKIRPLLENVFIEKTCNNRIGK
                     GTFACTQYVRNDIIEVSRNYTEDCWICKMDMQSFFMSIKKSLLVERIVSFIDINYSGD
                     DKEDIKWLIGIVLMDNPENNCVLKSPSSEWNKLSRNKSLFWVDDDLGLPIGNLISQLM
                     ANFYLNEFDHYVVEELGFTHYGRYVDDFYIIDTDKQKILRCIPLMRAKLEEVGITL"
BASE COUNT          721 a          444 c          539 g          613 t
ORIGIN      
        1 tcgcgacctc gatgatggca gcacttcagg cagcagggtt tacggccgca actgggtgga
       61 gctgtaccgc ctatcaggat tacaactgcg tggttgtcca gcaaaactgg tacactccta
      121 atgtgaccgt cttcaccgtt acggatgcag acttaaaggt gaataggact atcctgacag
      181 aaaattatca gaccgcgttg tcaggagttg ttacgccgta tggttatata cgcaggaatg
      241 atggtgttga cacttggtct gcaataacta atttagaaaa aggtctacta tattattccg
      301 taagcggaca agacttaaca ggtcaggcag ttggtgcaga ctctataatc aaggagtctc
      361 gattcaacgc aaacgataat cctttgcttg ttgccgctta cgggacttac aggaattaca
      421 tgcaagctaa gatgccgaga tatcctttca gcaagggagc gataattgac gacaatggca
      481 agggcaacac ggctgctctg gcctccatta tgtacacaga ctatgatggt gcactaaagc
      541 ctgctcatcc tgctgcatat aatgcgaaga attacggaat agcaactgat ggatatacaa
      601 ctggattcga ggcagggaac tggtatctgg aaagtgtcag gcttactaac atcctgatga
      661 aggatatcac ttatggcctc actggaataa caggcttaaa cgcagatcct gtaaatcgct
      721 caatatacgc tgctgggggt tcgatgatat cattggccgg atacccgtgg acaagcactg
      781 agtattccag caaggacgcc tggttctact tcggcttcac cggtcgcatg cgcaacctca
      841 ataagggcac cgtaaccgat gttcggccgg tctccgcttt tcaattacgt taacctttta
      901 acttacctgc gcagcaggtc tattatatga tagagaaatt acagatatac aaagactcat
      961 acctactcgc aggtaaactg tatcaggcga tgccgcagat ggacaagatg caccggcatg
     1021 taataggtac gaaaatcctc gactgtgcat tggatctgtt caagtggata tcgcttgcaa
     1081 atcagtcaag ggaaaagggc gagagaatga ggtatctgga ttccttttta tcaacatttg
     1141 agcaacttag ggtttacctg aggatttgca acgattttaa attattaaaa ttaagcacat
     1201 tatcagatat atacttactg acaacgagta tctctaaaca gttatctgga tggagaaacg
     1261 caacaacgag gacataatta cagagtgaat tatggggagt gatatttatt tgaaaagggc
     1321 tatccgctct catttagagc taagaataaa aaacagaggt agtaacaact gagtattcca
     1381 gcaataacac ctggatctac aacggcaaca acggcaacac gaacaacaac aataagaaca
     1441 acgtaaacaa tgttcggccg gtctccgaat ttcgaatggt attatttgtc agtatatggt
     1501 aaaggcagaa gacatattaa cggcttacaa tgattgcagg aagaataaaa ggaattctgc
     1561 aagcacgatt gagttcgagt gtgactatga gtctaattgt gtgtctcttt atgaggagat
     1621 aaacaatagg acatatcatc ctacaaaatc cattgccttt atagtcacaa agccaaggag
     1681 aagggagatt tttgctgcaa atttcaggga cagggtggtg caccacctta ttgatataaa
     1741 gatccgcccc cttttggaaa atgtctttat agaaaagacc tgcaacaata gaataggaaa
     1801 aggcactttt gcgtgtactc aatatgtcag gaatgatata atcgaggtct cccggaatta
     1861 tactgaggat tgctggattt gcaagatgga tatgcaaagc ttcttcatgt caataaaaaa
     1921 gtccctgttg gtggagcgaa ttgtgtcatt cattgatatt aactattccg gcgatgataa
     1981 ggaggatata aagtggctta ttggaatagt gttaatggat aatccggaaa acaattgcgt
     2041 gttaaaatcc ccctcttcag agtggaataa gttaagcagg aataagagtc ttttctgggt
     2101 tgatgatgat ctggggcttc caatcggaaa tctcatctct caattgatgg ctaatttcta
     2161 tctgaacgaa tttgaccact atgtggtgga agaattggga tttacccact atggcagata
     2221 tgtggatgac ttttacatca tagacacgga taagcagaag atattgagat gcattccttt
     2281 gatgcgggca aaattggaag aggtaggcat tacacta
//
LOCUS       DAOTQL010000212         2283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_37139_length_2283_cov_3.207361, whole genome shotgun sequence.
ACCESSION   DAOTQL010000212 DAOTQL010000000
VERSION     DAOTQL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37139_length_2283_cov_3.207361"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="P5293_08750"
     CDS             <1..364
                     /locus_tag="P5293_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HRR50021.1"
                     /translation="IDHEPVDDLSFQGKTVAIIENSGCMVCHSEDPKLPWYNKVPLIG
                     NKIRNDSKKGLSSINLRPYYESVLEAGLIDKNAAIRIDSVLISRTMPPTSYTILRPGS
                     RVNPKEREIILEWNRLHQ"
     gene            complement(385..1590)
                     /gene="hydF"
                     /locus_tag="P5293_08755"
     CDS             complement(385..1590)
                     /gene="hydF"
                     /locus_tag="P5293_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRR50022.1"
                     /translation="MSKVRHIGVFGRRNTGKSSLINILVGQDVAIVSNVPGTTTDPVR
                     KRMEINGLGPVVFIDTAGIDDSGVLGEQRVEKTKSVIKIIDLAILIFTNNQIEKYELD
                     LISSFREEGIPFIMLHNQSDIVPLDPELALDLSEKENIDVLEFSCSLLDEDDQNKAID
                     TLISMIVFELQKSEAVSKTILEGLVEKGDHIVLVCPIDSEAPEGRLILPQVNAIRDAL
                     DRKAVAIVIQPEQLGDYLKYLSVKPKLVVTDSQVFEMVSKIVPEDIPLTGFSLLLARV
                     KGAFQYYLDGIYNIDNLKDGDKILILESCTHHATCDDIGRVKIPNLLKKKSGKQLTFD
                     IVGGLDTLPEDICSYSMVIQCGGCMITDRQLQSRLRPAIKAGIPVANYGMVIAYCTGI
                     YNRAIEPLK"
     gene            1713..1844
                     /locus_tag="P5293_08760"
     CDS             1713..1844
                     /locus_tag="P5293_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50023.1"
                     /translation="MKKKAKQIITWSIVLIIIILVVDIACNIDGFVEGFKAGSNLAK"
     gene            1900..>2283
                     /locus_tag="P5293_08765"
     CDS             1900..>2283
                     /locus_tag="P5293_08765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRR50024.1"
                     /translation="MINQFQDLGLKDNLIKAVSDLGFTTPTEVQQRIIPKLINETGDL
                     VCLAQTGTGKTAAFGLPLLQSINTQSKNTQVLILSPTRELCRQISQDLDNYGKYIENL
                     NIVSVYGGAGIDGQIKLIRKGAQIIV"
BASE COUNT          764 a          460 c          373 g          686 t
ORIGIN      
        1 aattgaccat gaaccggtag atgatctttc ttttcaagga aaaacagtag ccattattga
       61 aaactcaggt tgtatggtat gccacagtga agatccaaaa ttgccatggt acaacaaagt
      121 accacttatt ggcaacaaaa taagaaacga ttctaaaaag gggctttcaa gcataaacct
      181 ccgtccctat tatgaatccg ttcttgaagc cggcctcatt gataaaaatg cagccataag
      241 aattgacagt gtccttattt cccgaacaat gcctcctacc tcttatacca ttttaagacc
      301 agggtcaaga gttaatccaa aagaaaggga aattatcctt gaatggaata gactgcatca
      361 atagtacaaa atttattggt ccttttattt caaaggctca attgccctat tgtaaatacc
      421 tgtacaataa gctatcacca tgccataatt agcaacagga atccctgctt ttatagcagg
      481 tcttagtctc gactgcagtt gtctgtctgt tatcatacaa cctccacatt gaatcaccat
      541 ggaataacta caaatatcct caggaagagt atcaagccct ccaacaatgt caaaagtcaa
      601 ttgttttcca ctctttttct tcaataaatt tggaatttta acccttccaa tatcatcgca
      661 tgtggcgtga tgtgtacatg attcgagaat tagtatctta tcaccatcct tcaggttgtc
      721 aatgttataa atcccatcca aatagtattg aaaagctcct tttacccgtg caagcaacaa
      781 actaaaacca gtaagaggaa tgtcttccgg aacaatttta cttaccattt caaatacctg
      841 actatcggtc actactaatt taggtttgac cgaaagatat tttaaataat ccccaagttg
      901 ctccggttga ataactatgg ccaccgcctt tcgatcgaga gcgtctctga tagcattaac
      961 ttgaggaaga atcaacctgc cctcaggagc ttcgctgtca atagggcaaa caagaacaat
     1021 gtgatcccct ttttctacca ggccttcaag aatagttttt gagacagcct ctgatttctg
     1081 aagttcaaaa acaatcattg aaattaaagt atcaatagct ttattctgat catcttcatc
     1141 aagaagagaa catgaaaatt ctaacacatc aatattttct ttttcactca aatccagagc
     1201 aagttcagga tcaagtggaa cgatatcaga ctgattgtga agcattataa aaggtatgcc
     1261 ctcttccctg aaactgctta tcaaatcaag ttcatacttc tcaatctgat tattagtaaa
     1321 aatcaaaatt gccagatcaa taatttttat cacagactta gttttctcga ccctctgttc
     1381 tccaagcact ccactatcat ctatacctgc agtatcgata aaaaccacag gtccaagtcc
     1441 gttaatctcc attcgcttac gaacaggatc tgttgttgtt ccaggtacat tggaaacaat
     1501 tgccacatct tgtccaacga gtatgttgat taaagagctt tttccggtat ttctacgtcc
     1561 aaaaactcct atatgtctga ctttgctcat aattataata ttggaaaaaa attaaaaaaa
     1621 gataaatttt cttttcaaag gtaatatttt tctaattatg aagtcgtttg tgggtagaat
     1681 aagctatgtt tgagcaaaac aaagaaatca aaatgaaaaa aaaggcaaaa caaatcatta
     1741 cttggagtat tgtactcatt attattattt tagttgttga tatcgcatgc aatatcgatg
     1801 gatttgtaga aggatttaag gctggaagta acctggctaa ataaataatt gctggttatt
     1861 agagaaatta gtataccttt gccgccttaa aataaataga tgataaatca atttcaagac
     1921 cttggtctta aagacaactt gatcaaggca gtgtcagacc ttggttttac tacccctaca
     1981 gaggttcagc aaagaattat accaaaactc ataaacgaaa ccggtgattt agtgtgcctt
     2041 gcccagactg gaacaggaaa aactgcagct tttggattgc ctcttttgca atccatcaat
     2101 actcaatcca aaaacactca ggtgcttatt ttaagcccta caagagagct ctgcagacaa
     2161 atctcccagg atttggataa ttacgggaaa tatattgaaa atctcaatat tgtctctgtt
     2221 tatggtggcg ccggcattga tggccaaatt aaactaatca gaaagggagc acaaattata
     2281 gtc
//
LOCUS       DAOTQL010000213         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_37433_length_2270_cov_5.471332, whole genome shotgun sequence.
ACCESSION   DAOTQL010000213 DAOTQL010000000
VERSION     DAOTQL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37433_length_2270_cov_5.471332"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..555)
                     /locus_tag="P5293_08770"
     CDS             complement(142..555)
                     /locus_tag="P5293_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50025.1"
                     /translation="MMIHPPNHWQVSNEDKNLFPVQFYKLEVPFAFPDEKKYVAFFAY
                     ARGKIYKLVRINYDGKDYIISTASFTGFSIGQATLYIKDITLRKNGGIGSILPENHFN
                     IIEDIIYCYNEKIGVFTPKTQGPIGLDDKLFTIDE"
     gene            complement(597..800)
                     /locus_tag="P5293_08775"
     CDS             complement(597..800)
                     /locus_tag="P5293_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50026.1"
                     /translation="MSTKDVKKYLVEWIYRQLLFIITLTFIFGVCAGVYGGKMLYEWR
                     MKQATTLKAFVYDKEIYDLKLRP"
     gene            complement(811..1332)
                     /locus_tag="P5293_08780"
     CDS             complement(811..1332)
                     /locus_tag="P5293_08780"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRR50027.1"
                     /translation="MYHINIHYKQHKHYLVLFLSFFLLFYFIHDFYISLPVPVDLSGG
                     GQTEKIEKILVAAGAPKDRIPVLRDAVATASGHTGFSPALIVALMKTESTFKKNAISP
                     LGYKGEMQTPFATRFSEVNVLYGVKILQEKLLYADGDILLALALYKGGNNSMAKRQAK
                     ETYKLYKQLAKLS"
     gene            complement(1714..2103)
                     /locus_tag="P5293_08785"
     CDS             complement(1714..2103)
                     /locus_tag="P5293_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50028.1"
                     /translation="MTKFIWTAATVFVMFLIFHVYAEAQQIVPQWELIGKSDQGEFVY
                     TDLKSKQTIKEPKYSSAWFRFVKDDEDIQIRFYSTFENKAVCYDLIRLNGKLIPVTEK
                     TCFLVQKNTIGEEIFRVLIVFYIVSNE"
BASE COUNT          686 a          483 c          332 g          769 t
ORIGIN      
        1 ggttttattc ctgccttgga gaaggaaaac cttctctgat tttctcaatc tctggtcact
       61 cgtaccctca cccgtggcgg cactatctcc acccatataa attacattat tatcaacata
      121 tcctactaca caactcatat atcattcatc aattgtaaac agtttatcgt caagaccgat
      181 tgggccttgt gtttttgggg taaaaactcc tattttttcg ttatagcaat aaataatatc
      241 ctcgataata ttaaaatgat tttccggtaa aatagaacca atacccccgt tttttctcaa
      301 ggtaatatcc ttgatataaa gggtggcttg tcctatagaa aatccggtaa aggaggcagt
      361 agaaataata taatcctttc cgtcataatt gattctcacg agcttgtaaa tcttaccccg
      421 tgcatatgca aagaaggcaa catatttttt ctcatccgga aaggcaaagg gaacttctaa
      481 tttataaaat tggacaggaa acaggttttt gtcctcattg gaaacttgcc aatgatttgg
      541 tggatgtatc atcattattt caccacttca ttttataata acttcacaga aatacattac
      601 gggcgtagct tcaaatcata aatttccttg tcatagacaa aggccttcaa cgtagtggcc
      661 tgtttcattc tccactcata aagcatcttg ccaccataaa caccagcaca cactccaaaa
      721 atgaaagtca atgtaataat aaaaagaagc tgtctatata tccactccac aagatatttt
      781 ttcacatctt tcgtactcat aacaacctcc ttatgatagt tttgctaatt gtttatacag
      841 tttataggtt tctttagcct gtcttttcgc catagaattg ttcccacctt tatacaaggc
      901 aagagccaaa agaatatctc catccgcata cagtaacttc tcttgaagaa ttttcactcc
      961 atagagaaca tttacttcac taaatcttgt tgcaaaagga gtttgcattt cacccttata
     1021 tcctaatggg gaaatagcgt tcttcttgaa tgttgattct gttttcatca gagcaacaat
     1081 caatgccggc gaaaatccgg tatgaccaga tgcggtagca accgcatccc tcaatactgg
     1141 tattcggtct ttcggtgcac cggccgccac cagtattttt tctatcttct ctgtctgccc
     1201 gcccccactc aaatcaaccg gaacaggtaa cgaaatataa aagtcgtgaa taaaataaaa
     1261 gagtaggaag aagcttaaaa acaatacaag ataatgtttg tgctgtttat aatgtatgtt
     1321 tatatggtac attatttcat cttctccttg taattgttaa tttttcttga tacaatctct
     1381 ccgtgacctg ctacggggca agccccgtag cttcctttct tcatcgcttt accctaacgg
     1441 gcaaccgttc tgaaaggctt tgtttttctt cgattccagg tccttgaaac cgaatgtatc
     1501 caaatttctt tatgtttttc atccctttcc tatttatctt tttctatcct cttttatagg
     1561 ataacataat ttaagtaact tgtaaagtag aaaaaagacc aataatcttg aatatataga
     1621 caaaaacagg gagaaatctt atattgtaat tctccctgtt tttgttgttt ttccatagtt
     1681 tttcactaaa agaatgagtt ttttctccac aaattattca ttactgacaa tataaaacac
     1741 aatcaatacc cgaaatattt cctcacctat agtgttcttc tggacaagga aacaagtctt
     1801 ttcagtaacc ggaattaatt ttccatttaa tcttataagg tcataacaga cggccttatt
     1861 ctcaaaggta gaataaaatc ttatctggat atcttcatca tcctttacaa accgaaacca
     1921 agcagaagaa tattttggtt cttttattgt ctgtttggat ttcaaatcgg tataaacaaa
     1981 ctctccctga tcactcttac caattagttc ccattgtgga acaatctgtt gcgcctcagc
     2041 atacacatga aaaattaaaa acattacaaa aactgttgcc gctgtccaga taaatttagt
     2101 catagtttag ctccatagac aaaagaagta tttttcgaag agtttccacc cttctttgta
     2161 tttctcaatt tcttcatcgg tggcaatccg tttgccgtac ttgtcacaga tgtttttctg
     2221 tacccgttct tttacttcca gatatctttc ttttgattcg aaaaactctt
//
LOCUS       DAOTQL010000214         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_37587_length_2263_cov_3.800725, whole genome shotgun sequence.
ACCESSION   DAOTQL010000214 DAOTQL010000000
VERSION     DAOTQL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37587_length_2263_cov_3.800725"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="P5293_08790"
     CDS             complement(<1..392)
                     /locus_tag="P5293_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRR50029.1"
                     /translation="MNILRKIIDNVKPTFSKGGKLGFLHSTFEAFEAFLFVPDSVTTN
                     GVHIRDALDLKRTMSVVIIALMPALLFGMWNTGYQYNLSIGGSMTFWQMFWFGFLKIL
                     PLIVVSYVVGLAIEFTSAQIRGHQVNEG"
     gene            complement(403..1776)
                     /locus_tag="P5293_08795"
     CDS             complement(403..1776)
                     /locus_tag="P5293_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HRR50030.1"
                     /translation="MSNHIRLRRGLNIPLKGKASNQITKNISSNIVAVKPSDFRNFSP
                     KLLVKEGDAVLAGSPVLADKKNLSIRLCSPVSGTVKEIVRGEKRKLLAVLIENDHKNE
                     HLQFTTLSKNAKKEEIIERLLEGGLWPTIKQRPYGIIPNPGVEPKSIFISGFDSSPLA
                     PDLDFTLKRETEAIQAGIDVLNLLTKGGIHFSLNIDNFAGSPFHRIERVIFHTFEGPH
                     PAGNVGIQINHVDPINKGDIVWTTDLYSLAAIGKLFTRGIYDVKRIIAITGPRCINPS
                     YVECLPGMCMSDIKEYSDENSEVKNNNLPVRWISGNVLTGVNVGANGYTGFFHNQVTL
                     ISEGNYYEMLGWIKPFRSKKFSISRTYFSWLNCKKQYELDTNTNGDERAFVMTGVYEK
                     VLPMDIYPTYLLKAILAEDIDNMEALGIYEVIEEDFALCEFVCPSKIKIQEIISKGID
                     LMLKEMA"
     gene            1829..>2263
                     /locus_tag="P5293_08800"
     CDS             1829..>2263
                     /locus_tag="P5293_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRR50031.1"
                     /translation="MKEEFKTIFEGLNSAQREAVERFEGPSLIIAGAGSGKTRVLTCR
                     IANILAHGHRAGSVLALTFTNKASREMKERIAGIVGYDVARYLWMGTFHSIFVKFLRE
                     SADLLGFPKNFTIYDTADSRSALRGCIKELSLDEKVYKPNEVL"
BASE COUNT          613 a          423 c          497 g          730 t
ORIGIN      
        1 tatccttcat tgacttgatg tcctctgatc tgtgcggatg taaactcaat ggcaagccct
       61 acgacgtatg aaactacgat gagtgggagt atcttgagaa acccgaacca aaacatttgc
      121 caaaaagtca ttgagcctcc gattgacaga ttgtactgat aaccagtatt ccacatgcca
      181 aaaagcaatg cgggcatcag agcaattatg acgacggaca ttgtgcgttt gagatccagt
      241 gcgtctctga tgtgaactcc gtttgttgtg acagaatcgg gtacaaaaag gaatgcttca
      301 aatgcttcaa atgtggagtg aagaaaaccc aatttgccgc ctttgctgaa ggtgggctta
      361 acgttgtcta ttatttttct tagtatattc atctctttgc tgttaggcca tttcttttaa
      421 cattaagtca atacctttag aaataatctc ctgtatctta attttggatg ggcagacaaa
      481 ttcgcaaagg gcaaaatcct cttcgattac ttcatatatg cccaatgcct ccatgttgtc
      541 aatatcttcg gcaagaatcg ccttgagaag ataggtggga taaatgtcca tcggtagaac
      601 tttttcgtag actcccgtca tgacaaatgc tctctcatcg ccattggtgt tggtgtcgag
      661 ttcatattgt tttttgcagt taagccatga aaagtaagtg cgtgatatgc tgaacttttt
      721 ggatcgaaat ggttttatcc acccaagcat ctcgtaataa ttgccttctg aaataagagt
      781 gacctggttg tggaagaatc ctgtgtaacc attggctcct acgttgactc ctgtaaggac
      841 atttccgcta atccatctta caggtaaatt attgtttttt acttcactgt tttcatcgga
      901 gtattccttt atatcgctca tgcacatacc gggaagacat tctacataac tcgggttgat
      961 acatcttgga ccggtaattg caatgattct tttgacgtcg tatattccac gggtgaatag
     1021 ttttcctatg gcagccaatg aatagaggtc tgttgtccag acaatgtcac ctttatttat
     1081 cgggtcaacg tgatttatct gtattccgac gtttcctgca ggatgaggtc cttcaaatgt
     1141 gtggaaaatt actcgttcaa ttctgtgaaa cggacttcct gcaaaattgt caatattgag
     1201 gctgaaatgt attcctcctt ttgtgagaag attcaaaaca tcaatacctg cctggattgc
     1261 ttctgtttct ctcttaaggg tgaaatcgag gtcaggtgca agtggtgaag aatcaaatcc
     1321 ggagataaag atagacttag gctcaacacc cggatttggg ataataccgt agggacgttg
     1381 tttgatagta ggccaaagtc cgccttcaag caacctttcg ataatctctt cttttttggc
     1441 atttttgctt agagtcgtaa attgcagatg ttcatttttg tgatcgtttt caatgagaac
     1501 cgcaagcagt ttacgctttt ctcctctaac tatctccttg actgtcccgc tgacaggcga
     1561 acacagtcgt atagagagat tctttttgtc tgccagaact ggtgaacctg ccaaaactgc
     1621 atcaccttcc ttaacaagga gcttgggact gaaatttcta aagtcggagg gtttcaccgc
     1681 tactatattc gaagagatat tttttgttat ttggtttgaa gcttttcctt ttaacggaat
     1741 gttcaaacct ctgcgtaatc gaatatggtt agacattatt gtgaaaaatt ttgcggcaaa
     1801 gatacacaat atttataatt ttgctcaaat gaaagaggaa tttaaaacaa tattcgaggg
     1861 gttgaatagt gctcaaagag aggcagttga gcgtttcgag ggtccttctt tgataattgc
     1921 cggtgcaggt tccggtaaaa caagggtact tacttgccga atagctaata ttcttgctca
     1981 tggacacaga gctggaagtg tgcttgcttt gacttttact aataaggctt ccagggaaat
     2041 gaaggagagg attgcaggta tagtgggtta tgatgtggcg cgatatcttt ggatgggtac
     2101 ttttcactcg atttttgtga agtttttgag ggaaagtgct gatttactgg ggtttcctaa
     2161 aaattttacg atttatgata cggcagattc aagaagtgct ttgaggggtt gtattaaaga
     2221 gctttctctc gatgagaaag tttataaacc aaacgaggtg ctt
//
LOCUS       DAOTQL010000215         2261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_37633_length_2261_cov_4.375793, whole genome shotgun sequence.
ACCESSION   DAOTQL010000215 DAOTQL010000000
VERSION     DAOTQL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37633_length_2261_cov_4.375793"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="P5293_08805"
     CDS             <1..758
                     /locus_tag="P5293_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRR50032.1"
                     /translation="SGINYDSDKYDHTELGMYLEAVASAAIKMKKRSPEYYEIINNIL
                     DSYLGKRPEKFTYNGVEYTPKSFLKYLGINLSDYVELTSFNHIPFYKASVLEIPDNWD
                     YGRLYNIPIDEFMEVINYALKSGYCIAWDGDVSEKGFSHKNGIAINPILEKGETEIDF
                     TKTYKEKQVDQQSRQQGFETFATTDDHLMLLTGISKDQNGNIYYITKNSWGPDGIFGG
                     YLNMSESYVRAKSISMLVNKNSIPKQIREKLGI"
     gene            771..1493
                     /gene="pssA"
                     /locus_tag="P5293_08810"
     CDS             771..1493
                     /gene="pssA"
                     /locus_tag="P5293_08810"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRR50033.1"
                     /translation="MRIARYLPNTITCLNLLSGTIAVIVGIEGNFLLSVYFVLLASLF
                     DFMDGFTARLLKAYSPMGKELDSLADLVSFGFAPAILLYKEYNLTQGMSVLTFMPLLI
                     IISSALRLAKFNIDTQQSENFIGMPTPTNAIIICMLIHFSLNNQFLGSITGSIWFIPV
                     FSIVMSALLISKIPMFSLKFKSLKWEGNEKRISIIIIAIILFVISIFFGITWSASLLA
                     TFVIYLLINVIEFSIKFFALKQ"
     gene            1490..>2261
                     /locus_tag="P5293_08815"
     CDS             1490..>2261
                     /locus_tag="P5293_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50034.1"
                     /translation="MNKEELSRQTILLEASVKASQILISNHSFYDAVNEVIEIIGNSL
                     PNKRVFVYELHKDLSGSNDLASLRFEKLNGEKAKRIFENSHQNIPLSFLPHNIIDDLL
                     KGKTVSSKEYSFNELGNFLLVPIFVEGSKWGYLGIESFDEEKVWSTSDKNVLNSIASS
                     LGQYINRHYQSLELIMAKELAEESEIYANSLFEQSPLCIHIFNTSGIIERVNKSSEHI
                     FSIPNDKIISKFNILTNRKSIEQGWNRFFKEALQTENEV"
BASE COUNT          787 a          374 c          375 g          725 t
ORIGIN      
        1 atagtgggat caactacgat tcagacaaat atgatcacac agagttagga atgtatcttg
       61 aagcagttgc aagtgctgcc ataaaaatga aaaaaagaag tccggaatat tatgaaatta
      121 ttaataatat acttgactct tatctgggaa aaagacctga aaaatttaca tacaacggag
      181 ttgagtatac acctaaaagt tttcttaaat acttgggtat taatttatca gattatgtag
      241 agttgacaag tttcaatcat atcccgtttt acaaggcatc tgttcttgaa atacctgata
      301 actgggatta tggcagattg tacaacatac caattgatga atttatggaa gtgataaatt
      361 atgcccttaa atcaggatac tgcattgcat gggatggaga tgtgagtgaa aaaggcttct
      421 cacacaaaaa tggaattgct attaatccta tccttgaaaa aggagagact gaaatagact
      481 ttactaaaac ttacaaagaa aaacaggtcg atcaacagag ccgccaacaa ggttttgaaa
      541 catttgccac tacagatgat catcttatgc tacttaccgg aatttcaaaa gatcaaaatg
      601 gtaatattta ctacataacc aagaattctt gggggcctga cggtatcttt ggcggttatc
      661 tcaacatgtc agaaagttac gttagagcga aaagtatatc aatgcttgta aataaaaact
      721 ccattcctaa acagattaga gaaaaactcg ggatataatt attaaattcg atgagaattg
      781 ccagatatct tccaaacaca atcacttgtc ttaacctttt gtcgggaaca atagccgtaa
      841 tagttgggat agaaggaaat ttcctactgt ctgtttattt tgttcttctt gcctctcttt
      901 ttgactttat ggatggattt actgcccgcc ttcttaaagc atattctcca atgggaaaag
      961 agcttgactc actggcagat ttggtaagtt ttggttttgc tccggcaatt cttttataca
     1021 aagaatacaa tctgacacaa ggcatgagtg tgttaacatt catgccttta cttattataa
     1081 tttcatcagc tctcagactt gctaaattta acattgatac tcagcagtct gaaaatttca
     1141 taggcatgcc tacaccaacc aatgcaatta taatttgcat gttaatacac ttttcactta
     1201 ataatcaatt tcttggatca ataaccggct ctatatggtt catacctgtt ttttccattg
     1261 taatgtctgc tcttctcata agtaaaatac caatgttttc actaaagttt aaatctttaa
     1321 aatgggaagg caatgaaaaa cgaatatcta taatcattat agctattatt ctttttgtta
     1381 tttcaatttt ctttggaatt acctggtcag catcactact tgcaacattt gtaatttacc
     1441 ttttaataaa cgttatcgaa ttttctataa aattttttgc tctcaaacaa tgaataaaga
     1501 agagcttagt agacagacaa ttttgttgga agcatcggtt aaggcgagcc aaatacttat
     1561 ttccaaccat agtttttacg atgctgtaaa cgaagttatt gaaataattg gcaattcctt
     1621 acccaataaa agagtttttg tttatgaact tcataaagat ttgtctggta gtaatgattt
     1681 ggccagcctt cgatttgaaa aattaaatgg agaaaaagca aagagaatat ttgagaattc
     1741 acatcaaaat attcctttaa gttttcttcc tcacaatatt attgacgatt tattaaaagg
     1801 aaagactgtc tcatcaaaag aatattcatt caatgaattg ggcaattttt tgctggtacc
     1861 aatatttgtc gaaggttcaa aatggggata tcttggaatt gagagttttg acgaggaaaa
     1921 agtatggagc acaagtgaca aaaatgtttt aaacagcata gcttcatcgc ttggacaata
     1981 tattaaccgc cattatcaaa gccttgaact tattatggca aaggaacttg cagaagaaag
     2041 tgaaatctat gccaattccc tttttgaaca gtctcctctt tgcatacata ttttcaacac
     2101 ttccggtata attgaaagag tcaacaagtc atcagaacat attttcagca tacccaacga
     2161 taaaataatt tcgaaattta acatactgac taacagaaaa tctattgaac aaggctggaa
     2221 cagatttttt aaggaagcat tacaaacaga aaacgaagtc t
//
LOCUS       DAOTQL010000216         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_37768_length_2255_cov_3.072273, whole genome shotgun sequence.
ACCESSION   DAOTQL010000216 DAOTQL010000000
VERSION     DAOTQL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37768_length_2255_cov_3.072273"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="P5293_08820"
     CDS             complement(<1..534)
                     /locus_tag="P5293_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRR50035.1"
                     /translation="MRRVVVTGMGIYSCIGQDLDTVRDSLMTGKSGIIVDPVREQMGF
                     RSYLTGFLPEPNLNSMLDRRKRVCMSQEASYAYLATLSALEQAKIDNDFLTKHEVGIL
                     YGNDSSALAVVEGVDILREKKNTTLIGSGSVFQSMNSSVTMNLSVIFKLKGINLTIAG
                     ACASGSHALGMGLMMIQS"
     gene            complement(543..1271)
                     /gene="fabG"
                     /locus_tag="P5293_08825"
     CDS             complement(543..1271)
                     /gene="fabG"
                     /locus_tag="P5293_08825"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HRR50036.1"
                     /translation="MKYALVTGGGRGIGRETCLKLSDMGYYVIINYNNSEQAALEVKE
                     NIVSKGGRASVIKFDVCDNLEVTKALEFWKNEHPDEYIEILVNNAGIRDDNLMIWMEP
                     ESWHRVINVSLNGMFYVTRLLIKDMLVNRFGRIVNVVSLSGIKGLPGQTNYSAAKGGV
                     IAATKALAQEVAKKGVTVNAVAPGFIDTDMTKNMDKETLRQQIPVGRFGTPGEVAALI
                     AFLASKDSSYITGEVISINGGLYT"
     gene            complement(1273..>2255)
                     /locus_tag="P5293_08830"
     CDS             complement(1273..>2255)
                     /locus_tag="P5293_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HRR50037.1"
                     /translation="TMHIREGLALANGTSMMTGIGLVNLYYSKKLLKWSILSSLMMNE
                     ITGSYDDFASERLNGAKPHYGQQYIAKELRKLMTTSKRLKKRESELYSGENHAPVFDE
                     KVQSFYSIRCIPQILGPVSDEIDNAEKVLINELNSANDNPIVDFRAGNIFHGGNFHGD
                     YVSFEMDKLKIAITKLTMLAERQLNYLYHDKINGILPPFVNLGVLGLNYGMQAAQFTA
                     TSTTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALIASKIIANSFEVLSILFISIL
                     QAVDYLKIQQDLSQNSRSVYNEMRNLCPIFVEDTPKYEEIASIVNYLKDN"
BASE COUNT          685 a          507 c          357 g          706 t
ORIGIN      
        1 agattgaatc atcattaacc ccatgccaag agcatgagaa ccactggcac atgcacctgc
       61 aatagttaga tttataccct ttagtttaaa aataacagaa agattcatgg taaccgatga
      121 attcatggac tggaaaaccg agccggatcc tatcaaggtg gtgtttttct tctccctgag
      181 tatatccaca ccctccacaa cagcaagtgc actgctgtca ttgccataaa gaatccctac
      241 ctcatgttta gtaagaaaat cattgtcaat cttagcctgt tccaatgcag acagtgtggc
      301 caaataggca tatgatgctt cctgagacat gcatactctc ttccttctat cgagcattga
      361 gttgagattt ggctcaggta aaaatcctgt taaatacgat ctgaaaccca tctgttccct
      421 gacaggatca actatgatgc ccgattttcc ggtcatgagt gaatccctga ctgtatcaag
      481 gtcctgacca atgcaagaat atatacccat tccggtgaca actactctcc tcatactgct
      541 ctctatgtat aaagtcctcc gtttattgaa attacttcac ctgtaatata tgatgagtcc
      601 tttgaggcta aaaaagcaat taaagccgca acttcccccg gagttccaaa tcttccaaca
      661 gggatttgtt gtctgagagt ctctttgtcc atattcttag tcatatctgt atcaataaaa
      721 cctggagcaa ctgcattgac tgtaacaccc ttctttgcca cctcctgagc aagagccttt
      781 gtagcagcta taactcctcc tttggctgca gaataattgg tttgacccgg caaacctttg
      841 attcctgata gagaaacaac attgacaatc ctcccaaatc tattgacaag catgtcttta
      901 atcaacagtc tggtaacata gaacatacca tttaatgaca cattaattac tctgtgccaa
      961 ctctcgggtt ccatccaaat cataagatta tcatctctga taccggcgtt gttaaccagt
     1021 atctcaatat attcgtcggg atgctcgttc ttccaaaatt caagagcttt tgtaacttcc
     1081 aaattgtcac aaacatcaaa ttttattact gatgctcttc ctcctttaga aacaatattt
     1141 tctttaactt ccaaagcagc ttgttcactg ttattataat tgatgataac ataatagccc
     1201 atatccgaca gtttcagaca tgtctcacgt cctattcccc ttcctccacc tgtcaccaaa
     1261 gcatatttca tatcagttat ccttaaggta gttaacaata gatgctatct cttcatactt
     1321 aggagtatct tctacaaata taggacaaag atttctcatt tcattatata cagatctgct
     1381 gttttgagaa agatcttgct gaatcttcaa ataatccaca gcctgcagta ttgaaataaa
     1441 aagaatggag agcacttcaa aagaattcgc aataattttt gatgcaataa gtgcagaatt
     1501 agtccccata cttacaatgt cctgattgtc attattattt gggatgctat gcacgtacat
     1561 cgggtttgag agagtttgac actcggcagt agtagaagta gcagtaaact gagctgcctg
     1621 cattccgtag ttaagtccta aaactcccaa gttgacaaaa ggtggtaaaa ttccatttat
     1681 cttatcatga taaagataat tcaactgtct ctcagcaagc attgtgagtt tagtaattgc
     1741 aattttcagc ttgtccatct caaaactcac ataatcacca tgaaaatttc ctccatgaaa
     1801 tatatttccg gctctgaaat caactatggg attgtcattt gcactgttta attcattaat
     1861 gagtactttt tcagcattat caatctcatc tgagacaggg ccaagtattt gggggataca
     1921 tcttattgaa tagaaactct gaaccttctc atcaaacaca ggagcatgat tctcacctga
     1981 ataaagttct gattctcttt tcttcaaccg cttagaagta gtcatcaact ttcttaattc
     2041 tttagcaata tactgctgac cataatgagg ttttgctcca ttaagcctct cagaagcaaa
     2101 atcatcatat gaacctgtaa tttcattcat catcagtgaa gaaagaatcg accatttaag
     2161 taattttttc gaataataaa gatttacaag cccaatccct gtcatcatgc tcgttccgtt
     2221 tgccaaggca agcccctctc ttatatgcat agtca
//
LOCUS       DAOTQL010000217         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_37865_length_2251_cov_4.753643, whole genome shotgun sequence.
ACCESSION   DAOTQL010000217 DAOTQL010000000
VERSION     DAOTQL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37865_length_2251_cov_4.753643"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2251
                     /gene="purL"
                     /locus_tag="P5293_08835"
                     /gene_synonym="purI"
     CDS             <1..>2251
                     /gene="purL"
                     /locus_tag="P5293_08835"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRR50038.1"
                     /translation="KDLDEEIFTINREPEKIKYITDIEEYNQQEGLALNIEEIEYLRK
                     LSDKLGRALTDSEVFGFSQVNSEHCRHKIFNGTFIIDGVEMPSTLFGLIKKTSKLNPN
                     RIVSAYKDNCAFIEGPDIQLFRPQSGDKPDFFEISQEKSVISLKAETHNFPTTVEPFN
                     GAATGSGGEIRDRMAGGRGSFPLAGTAVYMTSYPRFEVDGRYWECNEPRKWLYQTPEE
                     ILIKASNGASDFGNKFGQPLICGSVLTFEHSEFHIEYGYDKVIMLAGGVGFAKKEYSA
                     KNKPETGDKIAILGGENYRIGMGGGAVSSVDTGEYDNSIELNAIQRSNPEMQKRVYNA
                     IRALAESAHNSIISIHDHGAGGHLNCLSELVEDTGADIDLSKLPVGDSTLSAKEIIGN
                     ESQERMGLLIKPNDMEDLEKIASRERAPLYIVGETTGKKNFTFINSQTGEKPIDLQLE
                     DMFGSAPKTVIEDRNFKIHWDPLEYDEQDIEYYLEMVLQLESVACKDWLTNKVDRSVT
                     GLVANQQTAGEIQLPLNNLGVVALGYNNLEGIATSIGHAPAIALINAEAGSRMAISES
                     LTNIVWTPLKEGLRGISLSANWMWPCKNLGEDARLYSAVKAASDFAIALGINIPTGKD
                     SLSMTQKYPDGSKVLSPGTLIISAAGESSDIRRVVRPVLANRQDTRLFVVPFTIIDET
                     SFALGGSALAQITGQLGNEVPDIDNAEYFAECFNAVQEMISSDYIVAGHDISAGGMVT
                     TLLEMCFAN"
BASE COUNT          711 a          432 c          497 g          611 t
ORIGIN      
        1 ataaagatct ggacgaagaa atttttacaa ttaacagaga gccggagaaa attaaatata
       61 taactgatat tgaagagtat aatcaacaag aaggacttgc tctcaacatt gaagagattg
      121 aatatctcag aaaattgagc gataagcttg gaagagctct taccgacagc gaagtgtttg
      181 gattctcgca ggtcaactca gaacactgcc gtcataaaat ctttaatggt acatttatta
      241 ttgatggtgt ggagatgccg tccactcttt ttggtttgat aaagaagacc tctaagctta
      301 atcctaacag aatagtttca gcatataaag ataattgcgc atttatagag gggccggaca
      361 ttcagttgtt tcgtcctcaa agtggagata aacctgattt ttttgaaata agccaagaaa
      421 agagtgtaat ctcattaaaa gccgaaactc acaactttcc caccacagta gagcctttta
      481 atggagctgc aacaggtagc ggaggagaaa tccgggacag aatggccgga ggcagaggtt
      541 cttttcctct ggcaggcaca gccgtttata tgacctccta tccacgattt gaagttgatg
      601 gaagatactg ggaatgcaac gaaccacgca aatggttata tcagactcct gaagagattc
      661 ttatcaaagc ctcaaacggt gcaagtgatt ttggaaataa gtttggacaa cctcttattt
      721 gcggttctgt tttgactttt gaacactcag agtttcatat agagtacgga tatgacaaag
      781 taatcatgct tgcaggaggt gtcggatttg caaaaaaaga atattctgct aaaaacaagc
      841 ctgagacagg agacaagatt gcaattcttg gaggagaaaa ttacagaata ggaatgggag
      901 gtggcgccgt ttcctctgtt gacacaggag aatatgacaa ttcaattgaa ttgaatgcca
      961 tccagcgatc taatcctgaa atgcaaaaaa gagtttacaa tgcgattcgg gcgctggctg
     1021 aaagtgcaca taactccatc atatcgattc acgatcacgg agcgggcgga catctgaact
     1081 gtctgtcaga acttgttgaa gacaccggtg cagacataga ccttagcaaa cttcctgtag
     1141 gagattctac tctctctgcc aaagaaataa taggtaacga atctcaggag agaatgggtt
     1201 tacttattaa gcctaatgat atggaagatc tcgaaaagat agcatctcgt gagagagctc
     1261 ccctatacat tgttggcgaa acaaccggga aaaagaattt cacttttata aatagtcaga
     1321 ctggagaaaa accaattgat cttcaacttg aagacatgtt cggttctgca cccaaaacag
     1381 taattgaaga cagaaatttt aaaattcatt gggatcctct tgaatatgac gaacaggata
     1441 tagaatatta ccttgaaatg gttcttcaac tcgaatctgt agcttgcaaa gactggttga
     1501 caaataaggt tgatcgctct gttaccggac tggtggccaa tcagcagact gcaggcgaaa
     1561 ttcagttgcc tctaaacaat cttggtgtcg ttgctttggg ctataacaat cttgaaggaa
     1621 tagctacctc tatcggacac gctcctgcca tagcccttat aaatgcagaa gcaggaagta
     1681 ggatggcaat atccgaatct ctaacaaata ttgtttggac tccccttaaa gaaggactaa
     1741 gaggtatttc tctttcagcc aactggatgt ggccatgtaa aaacctggga gaagatgcaa
     1801 gattgtattc agcagttaag gcagctagtg actttgcaat agctttaggc atcaatattc
     1861 cgacaggcaa agattcgctt tcaatgacac aaaaatatcc tgacggcagt aaagtccttt
     1921 cacctggcac acttattatt tctgctgcag gagaaagctc tgacatcaga agagttgttc
     1981 gtcctgtcct tgctaatcgt caagacacta ggctatttgt tgtgcctttc acaataatcg
     2041 atgagactag ttttgctctc ggtggttctg cacttgcaca gattaccggc caacttggca
     2101 atgaagtccc tgacattgac aatgctgaat actttgcaga gtgcttcaat gctgttcagg
     2161 agatgatttc ttctgattac atagttgcgg gacacgacat atctgccgga ggaatggtta
     2221 caactttgct tgaaatgtgc tttgcaaact g
//
LOCUS       DAOTQL010000218         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_38976_length_2208_cov_4.013005, whole genome shotgun sequence.
ACCESSION   DAOTQL010000218 DAOTQL010000000
VERSION     DAOTQL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38976_length_2208_cov_4.013005"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="nfo"
                     /locus_tag="P5293_08840"
     CDS             complement(<1..564)
                     /gene="nfo"
                     /locus_tag="P5293_08840"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRR50039.1"
                     /translation="MKFIGAHVSAAGGVENAPFNAHQIEATAFALFTKNQRQWFSPPL
                     TKSSIELFKQRCIEYGYKPNQILPHDSYLINLGHPEKEGLEKSRNSFIHELQRCEQLG
                     LDRLNFHPGSHLNKIDIDTCLRIISDSINIALERTKGVTAVIENTAGQGSNLGHTFEQ
                     IEQILYYVEDKTRVGVCIDTCHSFAAGY"
     gene            complement(571..>2208)
                     /gene="recG"
                     /locus_tag="P5293_08845"
     CDS             complement(571..>2208)
                     /gene="recG"
                     /locus_tag="P5293_08845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRR50040.1"
                     /translation="ERLKSSGITNKQIAKYQSLLIKMTSDAIKETLPSYLIENKKLLP
                     LRHALKMIHFPENQASLNQAVFRIKLEELLYLQLSLLRQKKLRERYATGFMMPRIGKY
                     FNACYHNIPFELTNAQKKVIKEIRSDMVSGKQMNRLLQGDVGSGKTMVAVLCALLAAD
                     NGFQSCIMAPTEVLANQHYKSALKIVEGSGIEVALLTGSTKPFERNKILEELSQGKIH
                     ILFGTHALIEDRVKFNNLGFAVIDEQHRFGVEQRARLWTKSEEYPHILVMTATPIPRT
                     LAMTLYGDLDVSVIDELPPGRKPVRTIHATEAQRSRIYEFMKKQIKAGKQIFVVYPLI
                     KESEKLDYKNLQEGYEQIIQYFPAPEYVTAVVHGQQKNDDKAYDMSLFSEGKANILVS
                     TSVIEVGVDVPNATVMLIESAERFGLSQLHQLRGRVGRGSDESYCILMTGYKLSKESR
                     KRIELLCTTQDGFELAEADLRMRGPGDIEGTQQSGLAIDLHLSDLSKDSQILEEARRL
                     ATAILAEDPFLEDNDNKIFGEVLESLRKEVKDYSKIS"
BASE COUNT          584 a          446 c          406 g          772 t
ORIGIN      
        1 atatcctgca gcaaatgagt gacacgtgtc tatgcaaact ccaactctgg ttttatcttc
       61 aacgtaatac aatatttgtt caatctgttc aaatgtgtgt ccaaggttgc tgccttgtcc
      121 tgctgtattt tctataacag cagttactcc ttttgttcgt tcgagtgcaa tattaattga
      181 atcagaaatt attcgcagac aagtgtctat gtcaattttg ttcaaatggc ttccgggatg
      241 gaagttcagc ctgtcaagac cgagctgttc acatcgctgc agttcatgaa taaaagagtt
      301 tctggatttt tcaagtccct ctttttctgg atgtcccaga tttatcagat aactgtcatg
      361 tggaagtatc tgatttggtt tgtatccata ttcgatgcat ctctgtttga aaagttcaat
      421 actgcttttt gtcagaggag gtgaaaacca ttgcctctgg ttttttgtaa agagagcgaa
      481 agctgttgct tctatctgat gtgcattaaa aggagcattt tcaactcctc cggctgcact
      541 tacatgtgct cctataaatt tcataatagt ttaacttatt tttgagtaat cttttacctc
      601 ttttctcaga ctttctaata cttcgccaaa aattttattg tcattgtctt caagaaatgg
      661 atcttctgct aaaattgcag tggccaatct tcttgcttct tcaagaattt gagaatcttt
      721 agataaatcc gaaaggtgca aatcaattgc aagtccactt tgttgagtgc cttcaatgtc
      781 tccaggacct ctcattctca aatcagcctc agcgagttca aacccgtctt gtgttgtaca
      841 aaggagttca attctttttc ttgattcctt acttaatttg tatccggtca tcaaaataca
      901 gtatgattca tcactgcctc ttcccacccg tcctctcaac tggtgcaact gagacagacc
      961 aaatctttct gcactttcaa tcaacataac tgtagcatta ggaacatcta cgccaacttc
     1021 aataacagat gtggatacca gaatgtttgc tttgccttct gaaaaaagtg acatgtcata
     1081 tgctttatcg tcattcttct gctgtccatg tacaacggca gtaacatatt caggtgcagg
     1141 aaaatattga ataatctgtt catatccttc ctgcaggttt ttatagtcca gtttttcact
     1201 ttccttaata agaggataaa ctacaaaaat ctgtttgccg gccttaatct gttttttcat
     1261 gaattcataa atcctgcttc tttgagcttc agtagcatgg attgttctta ccggttttct
     1321 gccaggcgga agttcatcta tgactgagac atcaagatct ccataaagag tcatggcaag
     1381 ggtgcgaggg ataggagttg ctgtcattac aagaatatgc gggtattctt ctgattttgt
     1441 ccacagtcgc gctctttgtt caactccaaa cctatgttgc tcatctatta cggcgaatcc
     1501 gagattgttg aatttaactc tatcttcaat aagtgcatgt gtgccgaaaa gtatatggat
     1561 tttgccttga gacagttctt ctaaaatctt atttctttca aacggctttg tgcttccggt
     1621 aagcaaggcg acttcaatcc ctgatccctc aacaattttt aatgcacttt tataatgttg
     1681 atttgcaagt acttctgtag gggccataat gcaggactga aatccgttgt ctgcagcaag
     1741 caaagcgcaa agaacagcaa ccatggtttt cccactgccg acatctcctt gaagtaatct
     1801 gttcatctgt ttgccgctta ccatgtcaga ccggatctct tttataactt ttttttgagc
     1861 atttgttagt tcaaatggga tgttatggta acaggcatta aaatattttc ctattcgagg
     1921 catcataaat cctgtcgcat atctctctct taattttttt tgacgtagaa gagaaagttg
     1981 aagataaagc aactcttcta acttgattct gaaaaccgcc tgattcaatg atgcctggtt
     2041 ctcaggaaag tgtatcattt ttagggcatg tcggagggga agaagttttt tattctcaat
     2101 caagtaagaa gggagtgttt cttttatagc atcacttgtc atctttataa ggagagactg
     2161 gtatttagca atttgtttgt ttgtgatacc acttgacttg agcctttc
//
LOCUS       DAOTQL010000219         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_39706_length_2180_cov_4.786353, whole genome shotgun sequence.
ACCESSION   DAOTQL010000219 DAOTQL010000000
VERSION     DAOTQL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39706_length_2180_cov_4.786353"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="P5293_08850"
     CDS             complement(<1..386)
                     /locus_tag="P5293_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRR50041.1"
                     /translation="MKYYIIAGEASGDLHGSNLMKALFEVDPEAQIRFWGGELMASVG
                     GTLVKHYKQTAVMGLFDVISKLGPIIRNLQFCKKDIVEYQPDVIILIDYPGFNFKIAK
                     FAKKHSLRVFYYIPPTVWGWKEWRIK"
     gene            complement(383..1150)
                     /gene="surE"
                     /locus_tag="P5293_08855"
     CDS             complement(383..1150)
                     /gene="surE"
                     /locus_tag="P5293_08855"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRR50042.1"
                     /translation="MRKILITNDDGVKSKGISHLIEMMLPYGELTVVAPFEAQSGMSV
                     ALTIGKPLRLTELKSGNGLKVYACSGTPADCVKMAMNQLFQDELPDFLVSGINHGSNA
                     SIASLYSGTLGAAIEGTIYGIPSIGLSLCTHKQDADFSGVSHFGKIIIDNFIKNPVGP
                     DAYLNVNFPDLKPEEIKGIKFAKQGKGKWIKEFEQREDPYGFKYYWMTGEFLNLEPDN
                     DKSDNNVVDSGYVSVVAHKIDNTDYDEVKRLKSLWKI"
     gene            1273..>2180
                     /gene="gap"
                     /locus_tag="P5293_08860"
     CDS             1273..>2180
                     /gene="gap"
                     /locus_tag="P5293_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRR50043.1"
                     /translation="MNKIKVGINGFGRIGRLVFRAAQSRDDIQIVALNDLISVDYMAY
                     MLKYDTVHGKFDGTVEVKDGMLIVNGNPIRVTAEKNPGDLKWGEVGAEYVVESTGLFL
                     TKESAQLHIDAGAKRVIMSAPSKDDTPMFVYGVNHKKYAGESIVSNASCTTNCLAPVV
                     KVLHENFGIIEGLMTTVHATTNTQKTVDAPSAKDWRGGRAAGANIIPSSTGAAKAVGK
                     VLPEMKGKLTGMSFRVPTVDVSVVDLTCRLEKPATYDEIKAAMKKASENELKGILGYT
                     EDAVVSSDFITDPRTSIFDAGAGISL"
BASE COUNT          639 a          417 c          417 g          707 t
ORIGIN      
        1 cttttaattc tccattcttt ccatccccag acagtaggag gtatgtagta aaatactctt
       61 aatgaatgtt tcttggcaaa tttggctatt ttgaaattaa aacctggata gtcaatgaga
      121 attataacat caggttgata ttcaacaata tcttttttac aaaattgtag attgcgaata
      181 atgggtccta attttgaaat cacgtcaaat aatcccatca ctgcagtttg cttatagtgt
      241 tttacaagag ttccacctac tgaagccatc aattcacctc cccagaagcg aatctgagct
      301 tctggatcaa cttcaaaaag tgctttcatg agattggatc cgtgaagatc tcctgatgct
      361 tcccctgcaa tgatgtaata tttcatattt tccagagtga ttttagtctt tttacctcat
      421 cgtagtccgt attgtctatc ttgtgagcga caacactaac atatccactg tcaactacat
      481 tgttgtcaga tttatcattg tcaggttcaa ggttgaggaa ttctccagtc atccagtaat
      541 acttgaaacc ataaggatct tctctttgtt caaattcttt tatccactta ccttttccct
      601 gtttggcaaa tttaatacct tttatctctt caggtttgag atccggaaaa ttaacattta
      661 aataggcatc tggtccaacc ggattcttaa taaaattatc aattattatc tttccgaaat
      721 gtgaaacacc tgaaaaatcg gcatcttgtt tatgtgtgca tagtgaaagt ccaatagacg
      781 gaatgccgta aatagtgcct tctattgctg cgccaagtgt cccggagtat aaagatgcaa
      841 ttgatgcatt tgagccgtga ttaatacccg atacgagaaa atcaggtaac tcgtcttgaa
      901 acaactgatt cattgccatt tttacgcaat ctgccggtgt tccagaacat gcgtaaactt
      961 tcaatccatt accactcttc aattctgtca atctcagcgg tttgccaata gtcagagcaa
     1021 cagacatacc tgattgtgcc tcaaaaggtg caacaactgt taactctcca tatgggagca
     1081 tcatctctat caagtgcgaa attccttttg atttaactcc gtcatcatta gtaatcagaa
     1141 tcttcctcat tttttattta aatcgatttc tttctttaat cttacaaaaa tagtaatttt
     1201 gccggagtat tccccaaaaa tgcttatatt tgcccaagat tataaagtgt taatctaata
     1261 aataattgca agatgaacaa aattaaagtt ggtattaacg gtttcggccg tataggccgt
     1321 ttggttttcc gtgctgctca gtcaagggat gatattcaga ttgttgcctt aaatgacctg
     1381 ataagtgtcg attatatggc ttacatgttg aagtatgata cagtccatgg aaagtttgac
     1441 ggtactgttg aggttaagga tggcatgctt atcgtaaatg ggaatcctat tagagttaca
     1501 gctgagaaaa atcctggaga tctgaaatgg ggtgaagtag gtgctgaata tgttgttgaa
     1561 tcaacaggtc tatttcttac aaaggagagt gcccaacttc atatagatgc aggtgctaaa
     1621 agagttatta tgtcagctcc ttccaaggac gatactccta tgtttgttta tggagtcaat
     1681 cataagaaat atgccggaga gtcaattgtt tcaaatgcat catgtacaac taactgtctt
     1741 gctcctgttg ttaaggtgct ccatgagaac tttggcatta ttgaaggatt gatgacgact
     1801 gttcatgcaa caaccaatac tcagaaaact gttgatgctc cttcagctaa ggactggaga
     1861 ggtggtcgcg ctgccggagc taatattatt ccttcttcta caggagctgc caaggctgtt
     1921 gggaaagtac tccctgaaat gaaaggcaag ttgactggga tgtcatttag ggttccaact
     1981 gtagatgtat ctgtggttga tcttacatgt cgccttgaga aacctgctac ttacgatgag
     2041 ataaaagctg ccatgaaaaa agcttccgaa aatgaactta aagggattct tggatacaca
     2101 gaggatgctg tagtttcatc tgattttatt acagatccgc gtacatctat tttcgatgct
     2161 ggagccggca tatcactcaa
//
LOCUS       DAOTQL010000220         2179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_39726_length_2179_cov_3.988230, whole genome shotgun sequence.
ACCESSION   DAOTQL010000220 DAOTQL010000000
VERSION     DAOTQL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39726_length_2179_cov_3.988230"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="P5293_08865"
     CDS             complement(<1..818)
                     /locus_tag="P5293_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRR50044.1"
                     /translation="MSKTFKIVVLAKQVPDTRNVGKNAMKEDGTVNRAALPAIFNPED
                     MNALEQALLIKDKFPGTRVELLTMGPLRAADIIREGYFRGADGGVLLTDRKFAGADTL
                     ATSYALSMAIRKINPDIIISGRQAIDGDTAQVGPQVAEKLGIPQITYAENIISIKDGT
                     ITVIRRLERGIEEVRSIYPVLITVNSSAPACRPRNAKQVMKYKHARTVSELNDKEDHS
                     YFPVTSFQELVIPEWGVADVGGEEEKYGLSGSPTKVKKIENIVFQVKESKRLTSS"
     gene            complement(833..1141)
                     /locus_tag="P5293_08870"
     CDS             complement(833..1141)
                     /locus_tag="P5293_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRR50045.1"
                     /translation="MDSDNEINIELSSEVALGSYSNFAIITHSSSEFILDFLSMLPGI
                     PKPQVTNRIIMTPEHAKRLMVALEDNIAKFENKFGEIVLYNEQEKIFPMGFGGNSAEA
                     "
     gene            1261..>2179
                     /locus_tag="P5293_08875"
     CDS             1261..>2179
                     /locus_tag="P5293_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRR50046.1"
                     /translation="MELSAKYDPSLTENKWYDYWLKYKFFHSEPDEREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLVRRARMMGKNACWVPGTDHASIATEAKVVAKLKSEGIEK
                     SNLTREEFLKYAWEWKEKHGGIILEQLKKLGASCDWNRTRFTMDFDLSKGVINTFVYF
                     YKKGWIYRGVRMVNWDPVGQTAISDEEVIHKETSSKLYYLKYFISSNGKPSEEYLVVA
                     TTRPETIMADTAVCINPEDERYFNLKGKRVFIPLINKEIPIIEDDYVSMDFGTGCLKV
                     TPAHDVNDYEIGLRHKLPVIDILDQFGRLN"
BASE COUNT          671 a          377 c          419 g          712 t
ORIGIN      
        1 gaagaggtaa gtctcttgga ctctttgact tgaaatacaa tattttcaat ttttttaacc
       61 tttgtaggag atccagaaag accatatttt tcttcttcac cgccaacatc tgcaactccc
      121 cattcaggaa taacgagttc ctgaaagctt gtgacaggga aataggaatg gtcttcctta
      181 tcattgagtt ccgaaacagt cctggcatgt ttatatttca taacttgttt tgcatttctt
      241 ggcctgcatg ctggtgcaga tgagtttacc gtaattaata caggataaat acttctgacc
      301 tcctcgatgc ctctttcaag tcttcttata acagtaattg tgccgtcttt aattgaaata
      361 atattttcgg cataagtaat ctgaggaatt cccaattttt cagctacttg agggcctact
      421 tgtgcagtgt cgccgtctat agcctgtctt cctgatataa ttatgtcagg attaattttt
      481 cttatagcca ttgaaagagc atatgatgtg gcaagtgtat cagctcctgc aaattttctg
      541 tcagttaaaa ggactccgcc atcagcaccc ctaaaataac cctctcttat aatatctgca
      601 gctcttaagg ggcccattgt cagtaactca actctggtac cgggaaattt gtccttaatt
      661 agtaaagcct gctcaagggc attcatgtct tcagggttaa atattgcagg aagtgcggct
      721 ctattaacag ttccatcctc tttcatggca ttctttccta cattgcgcgt gtcaggaacc
      781 tgctttgcaa gtacaacaat tttgaaggtt ttactcatgt tttgttaatt aattatgctt
      841 cagcggaatt gccaccaaag cccattggaa atattttttc ttgttcgtta tataagacta
      901 tctctccaaa tttgttttca aatttggcaa tattatcctc aagagcaacc attagtcgtt
      961 tggcatgctc gggtgtcatt attatcctgt tggttacttg tggtttaggt ataccaggaa
     1021 gcatactcaa aaaatcaagg ataaattcac tgcttgaatg tgtaattatc gcaaagtttg
     1081 agtatgaacc taatgcaacc tcactactta attcaatatt tatctcatta tcagagtcca
     1141 tgacgttttt tattaattta tttcctgcaa agatagtttt ttttcttaaa agcgaatttt
     1201 gagaagggtg attaaatatt taactttgaa gtttaattaa ttcatagata gatagagtat
     1261 atggaattat ctgcgaaata tgatccttca ttaacagaga ataaatggta tgactattgg
     1321 ttgaaataca agttttttca ttctgaacca gatgaaagag agccttatac tatagtaatt
     1381 cctcctccaa atgttacagg tgttcttcat atggggcaca tgttaaacaa cacaattcag
     1441 gatgtgcttg tcagaagggc aaggatgatg ggaaaaaatg catgttgggt acctgggaca
     1501 gatcacgcat caattgccac tgaagctaag gttgttgcaa aattaaaatc ggaaggcatc
     1561 gaaaaaagta acctgacaag agaagagttt cttaaatatg cctgggagtg gaaagagaaa
     1621 cacggaggaa taattcttga acagttaaaa aaattgggag catcttgtga ttggaacaga
     1681 acgaggttta caatggactt tgacttaagc aagggagtta ttaatacttt tgtttatttc
     1741 tacaaaaaag ggtggattta cagaggtgtt aggatggtta actgggatcc cgttggtcag
     1801 acagccataa gtgatgagga ggtaattcat aaggaaactt catctaaact atattactta
     1861 aagtatttta tatcaagtaa tggcaaaccg tctgaagaat atctggtagt tgcaactaca
     1921 agaccagaaa ctatcatggc cgatacggct gtctgcatta atccggaaga tgagcgatat
     1981 tttaatctta aaggtaaaag agtatttatt ccacttatta acaaagaaat accaataatc
     2041 gaagatgatt acgtctcaat ggattttggt acaggttgtc ttaaagttac tcccgctcac
     2101 gatgttaatg actatgaaat tggccttaga cataaactcc cggttataga tattttagat
     2161 caatttggaa gattaaatg
//
LOCUS       DAOTQL010000221         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_40878_length_2136_cov_4.098030, whole genome shotgun sequence.
ACCESSION   DAOTQL010000221 DAOTQL010000000
VERSION     DAOTQL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40878_length_2136_cov_4.098030"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="P5293_08880"
     CDS             <1..547
                     /locus_tag="P5293_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRR50047.1"
                     /translation="MKLFIDNWRWEGVPFYIRTGKEMPTKVTEIVIHFKKTPYFPFLG
                     GKTIPEANQLVIRIQPNEGAILKLDMKVPGPGFKLKQVSMDFTYDKLGILPTEDAYTR
                     LIEDCLNGDSTLFTRSDAVIESWKFFDKIIRFWKENDVPLYGYPAGTWGPPESETIFS
                     GNGNWTNPCKNLINTDLYCEL"
     gene            537..1217
                     /gene="pgl"
                     /locus_tag="P5293_08885"
     CDS             537..1217
                     /gene="pgl"
                     /locus_tag="P5293_08885"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HRR50048.1"
                     /translation="MNSNYLYAKELTENLNIEVRSKKSSFYLAISGGTGASILFKLWS
                     GMYQNIIQWENIDFFWVDERCVPPDHIESNYGNAVRDFFSKVDINPNRIHRIYGENDN
                     RKEAVRYSEEVCNIVPKYNLLPKFDMIILGIGDDGHTASIFPGQTHLLSCMDPYAPSV
                     NPYDGVKRVTMTAPLILNSEKAVFYLYGEAKSLIMEKILNFSGNECIYPASYLLTRRA
                     DIKIFWDR"
     gene            complement(1218..2003)
                     /locus_tag="P5293_08890"
     CDS             complement(1218..2003)
                     /locus_tag="P5293_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRR50049.1"
                     /translation="MSYKYKLGIALSGGGVKGFAHAGALKAIEEFGIRPEIISGTSAG
                     AIVGAMYASGLPPEKIIEIFRNRYINNFIEFTLPKAGLFGTAGFIKFLDENIPAKTFE
                     ELKIPLLVVATDFDHGKSVVFSSGELINKVMASACVPVFFKPITIDGINYVDGGLFMN
                     FPVSVIRKDCEKVIGINVSPLIADKYSKSIIGVVERTYHYIFRANTILEKRLCDVLVE
                     VEEAMQFKVFDLDKVDKIFELGYQVMKSKLEQKTDILKNYLIC"
BASE COUNT          684 a          366 c          389 g          697 t
ORIGIN      
        1 catgaaactc tttattgaca attggcgttg ggagggtgta ccattttata tcagaacagg
       61 caaggagatg ccgacaaagg tgaccgagat agttattcat tttaagaaaa caccatattt
      121 tccttttttg ggaggcaaga caatccccga agctaatcaa ttggtaatta gaatacaacc
      181 aaatgaggga gcgattttga aattagacat gaaagtacct ggtcctggtt ttaaacttaa
      241 acaagtaagt atggatttta cttatgataa gttgggcatt cttccaactg aagatgcata
      301 tacaaggctt attgaagatt gccttaacgg cgattcaacg ctttttacaa gaagtgatgc
      361 agtgatagaa agttggaaat tctttgacaa aatcattcga ttttggaaag aaaatgatgt
      421 tcctttatat ggttatccag caggaacatg gggaccacct gagagcgaaa ctattttctc
      481 gggcaacggc aattggacaa atccatgtaa aaacttgata aatacagatt tgtattgtga
      541 actctaatta tttgtacgcg aaggaactga ctgaaaattt aaacattgaa gtcaggtcaa
      601 agaagtcttc cttttatttg gctatttccg gaggaactgg tgcttcaatt ttgtttaaat
      661 tgtggagtgg aatgtatcaa aatattattc aatgggaaaa tatagatttt ttctgggtgg
      721 atgaacgttg tgtgcctccg gatcatatag aaagcaatta tggcaatgct gttagagatt
      781 tctttagtaa agtagatatt aatcccaacc ggatccacag gatttatggt gaaaatgaca
      841 atcgtaaaga ggcggtcaga tattctgaag aggtctgtaa cattgttcct aaatataatt
      901 tattgcccaa atttgacatg ataatacttg gtattggaga tgacggacat acagcgtcaa
      961 tttttcccgg tcaaacacat cttttgagtt gtatggaccc atatgcgcca tcagtcaatc
     1021 catatgatgg tgttaagagg gtaacaatga ctgcaccttt aatccttaac tctgaaaaag
     1081 cggtatttta tctttatgga gaagctaaaa gtttaataat ggaaaagata ttgaactttt
     1141 ccggcaatga atgtatatat cctgcttcct atttacttac aagaagagca gatattaaaa
     1201 tattttggga cagataatca acaaataaga taattcttca aaatatctgt tttttgttca
     1261 agtttagatt tcattacttg atatccgagt tcaaatattt tgtctacttt gtcaaggtca
     1321 aatactttaa attgcatggc ctcttcaact tcaacaagaa cgtcacaaag cctcttttca
     1381 agtattgtgt tggctctgaa aatatagtga tatgttcttt caactactcc tatgatgctt
     1441 tttgaatatt tatcagctat aagtggacta acatttatgc ctattacttt ttcacaatct
     1501 tttcttatga cagagacagg aaaattcata aataatcctc catctacata attaattccg
     1561 tcaatggtaa ttggtttaaa aaaaacagga acacatgccg atgccataac tttatttatc
     1621 agttctcccg aactgaaaac aacgctttta ccatggtcaa aatcagttgc tactactaaa
     1681 agggggattt ttaattcttc aaaagtcttt gcaggaatat tttcatcgag gaactttatg
     1741 aaacctgcag ttccaaacaa tccggcctta ggtaatgtga actcaataaa attgttaata
     1801 tatctgtttc tgaaaatttc aattatcttt tcaggaggaa ggcctgaagc atacatagcg
     1861 ccaacaattg cacctgcact tgtgccagat attatttcag gacgaatacc aaactcttca
     1921 atggccttga gagctccggc atgtgcaaaa cctttaactc ctcctcctga aagagcaatt
     1981 cctaacttgt atttataaga catattccag tatcttacgt gtggcagaag agtcaattgt
     2041 tttattttcg ccaaaatgaa cgtttctctt ctcgaatctt tttacaatct cgtcaatagt
     2101 ctccttgcca atgccgtttt cggatagtct tgtttt
//
LOCUS       DAOTQL010000222         2121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_41251_length_2121_cov_4.523717, whole genome shotgun sequence.
ACCESSION   DAOTQL010000222 DAOTQL010000000
VERSION     DAOTQL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41251_length_2121_cov_4.523717"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="P5293_08895"
     CDS             complement(<1..909)
                     /locus_tag="P5293_08895"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRR50050.1"
                     /translation="MKKIALLLLAVILISLTPLRADEGMWLLPLLEKLNQQKMKELGC
                     KLTAKDIYNVNGTSLKDAVIQFGNGCTGEVISAEGLIITNHHCGYGAIQKLSSVEHDY
                     LTDGYWAMSREQELPVYGLTVTFLESFTDVTDKINNAMSNAATSEEKVNAMNNIIAQL
                     KKEAIGNNNYLKANVVPMYAGNMYYLVVTKTFNDIRFVGAPPSSIGKFGADTDNWMWP
                     RHTGDFSMFRIYADKNNNPAPYSPENVPYQAKRHLNISIKGINHNDYAMIIGYPGRTN
                     RFMTSAEVRQTSDISNAITIFARGLRQ"
     gene            945..2090
                     /locus_tag="P5293_08900"
     CDS             945..2090
                     /locus_tag="P5293_08900"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRR50051.1"
                     /translation="MLDTFKILIVIDKFKGSLTSFQACFAIKKGIEKRWEDLFPSLNL
                     SIEALPMADGGEGTLDVVEKSIGGIRHYASVSDPLFREIKVPFLLKNKTAFIEMALCS
                     GLQLLTKDEYNPMITSTYGLGELILEAVRYGAEDIVIGIGGSATNDGGMGMLKALGYE
                     FFDRRGDEALSLNEVYEISDFNVSQSLHKVKFTVACDVTNPLLGEKGASIVYAPQKGA
                     TPEMVEKLEIGMRNYAEICERFTGRCCRDYPGSGAAGGIGFAFKLFFDAEIIPGWMIL
                     SRLTNMEKLISTADLVITGEGSLDQQSVSGKLVYGVCMTGIMYNKSIWAFCGINHLNV
                     NELSNLGISKVFEIITVARDIDDSIKNASYYLEQMAFNAISFLSPKP"
BASE COUNT          626 a          348 c          435 g          712 t
ORIGIN      
        1 ttgacgaagt cctcgagcaa atatcgtaat ggcatttgaa atatcacttg tttgcctcac
       61 ctctgcagag gtcatgaaac gattggttct gccagggtat ccaataatca ttgcgtagtc
      121 attgtgattt attcctttga tagagatatt taaatgcctt ttagcctgat atggtacgtt
      181 ttctggagag tatggtgctg gattattatt cttatctgcg tatattctga acatcgaaaa
      241 atcaccagtg tgtcttggcc acatccaatt gtcggtatca gcgccaaact tgccaatgga
      301 tgaaggaggg gctcctacaa atctgatgtc attgaatgtt tttgtcacta caagatagta
      361 catgttgcct gcatacattg gaactacatt tgcctttaaa taattattgt taccaatagc
      421 ctctttttta agttgagcaa ttatattatt cattgcatta actttctctt cagaagtagc
      481 agcatttgac atagcgttgt tgattttatc ggtcacatca gtaaaacttt caagaaaagt
      541 gacagtgaga ccatataccg gaagttcctg ctcccggctc attgcccagt atccatctgt
      601 aagatagtcg tgttctacac tgctaagttt ttgaattgct ccataaccgc agtgatgatt
      661 ggtaattata agtccttcag cagatatcac ttctccggta catccgttac caaactggat
      721 gacagcatct tttaagcttg ttccattgac attgtatata tcctttgccg tgagtttaca
      781 tccaagttct ttcatttttt gctgattgag cttttctagc aaaggaagca gccacattcc
      841 ctcatctgct ctaagtggag ttagcgagat aaggattaca gctaatagta ataatgcaat
      901 ttttttcatg aatttttatc tatttttaac aaaaataagc aaaattgtta gatacattta
      961 aaattctgat tgtaattgac aagtttaaag gaagtctcac atcttttcaa gcttgttttg
     1021 ccattaaaaa gggcattgaa aagaggtggg aagatttatt tccttcatta aacttatcaa
     1081 tagaagccct accgatggcc gacggaggag aaggcactct cgatgtcgtt gagaaaagta
     1141 ttggaggaat tagacattat gcttctgtaa gtgatccttt atttagagaa ataaaagttc
     1201 catttttact gaagaataaa acagcattta ttgaaatggc cttatgctct ggtcttcagc
     1261 ttttaaccaa agatgagtat aatcctatga taacttcaac ttatggtctt ggagagttaa
     1321 ttcttgaagc tgtaaggtat ggagctgaag atattgttat tggaattggg gggagtgcaa
     1381 ctaatgatgg tggtatgggt atgcttaaag ctctgggtta tgagtttttt gacaggagag
     1441 gagatgaagc attatcttta aatgaagttt atgaaatatc agatttcaat gtttctcaat
     1501 cattacataa agttaaattt accgttgcct gtgatgttac aaatcctctt ctgggggaaa
     1561 agggagcttc aattgtttat gcgcctcaaa aaggagctac tcctgaaatg gttgagaagc
     1621 tggagattgg catgagaaat tatgctgaga tttgtgaaag atttactggc agatgctgca
     1681 gggattaccc gggatctgga gctgccggtg gaattggatt tgcattcaaa ttattttttg
     1741 atgcagaaat tattcctgga tggatgatac tatcaaggct caccaatatg gagaaattaa
     1801 tttctactgc agatttggta ataacagggg aaggatccct ggatcaacag agtgtttcgg
     1861 gcaaactagt atatggagta tgtatgaccg gtataatgta taacaaatct atatgggcat
     1921 tttgtgggat aaatcatcta aatgttaatg aactcagcaa tttggggatc tcaaaagttt
     1981 ttgagataat aactgttgca cgagacattg acgactcaat caaaaatgct tcatattatc
     2041 ttgaacagat ggcttttaat gctatttctt ttctgtctcc caaaccttga gtatttctgc
     2101 tatatatagt ttatgaagtc c
//
LOCUS       DAOTQL010000223         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_41619_length_2108_cov_4.633707, whole genome shotgun sequence.
ACCESSION   DAOTQL010000223 DAOTQL010000000
VERSION     DAOTQL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41619_length_2108_cov_4.633707"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="P5293_08905"
     CDS             <1..604
                     /locus_tag="P5293_08905"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRR50052.1"
                     /translation="AEAGADFIKVGIGGGSICITREQKGIGRGQATALIEVAQARDEY
                     FERTGIYIPICSDGGIVQDYHMVLALAMGADFLMMGRYFARFDESPTKKLMVGGTYVK
                     EYWGEGSNRARNWQRYDAGGTDSLKFEEGVDSYVPYAGKLRDNLDITLGKVKATMCSC
                     GALTIKELKKLAKITLVSSTSIIEGGTHDVILKDTNSSGI"
     gene            771..1208
                     /locus_tag="P5293_08910"
     CDS             771..1208
                     /locus_tag="P5293_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRR50053.1"
                     /translation="MSLVKFSNQFPTLFDRFFDNDLFDWSNKNFSLTNTTLPSVNILE
                     DDNSYEVEMAAPSLNKDDFKIELNNDVLTISSEKQTENETKEGQRYTRKEFSYQSFSR
                     SFTLPDSVDNDKIKAKYENGVLRVIIPKREDAKPRPVKQIAIE"
     gene            1229..1678
                     /locus_tag="P5293_08915"
     CDS             1229..1678
                     /locus_tag="P5293_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50054.1"
                     /translation="MKKLALLLVSFSIVVAGCYGQSDKKGQPATSVQPKANIKVNKEY
                     DKNGRLIRYDSTYTYVYPGTDSLFTDEFFKEFEQRFNSYFISPRTYMFPVYPFPQMSP
                     FYEFFQNDSSMVKYNQYRKQIEKLYKEIDSIRNAFYFEQFNSKTKKK"
     gene            complement(1703..>2108)
                     /locus_tag="P5293_08920"
     CDS             complement(1703..>2108)
                     /locus_tag="P5293_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRR50055.1"
                     /translation="MLFLYNVEITRVQCAFIDTKEIDRIVDFISSQQGYATAHMLPEY
                     VGDENDGYGVGEVDLSKRDKMFEEAARLIVQYQQGSTSLIQRRLNLGYNRAGRIMDQL
                     EAAGIVGPSEGSKARQVLISDYDSLDRILNSL"
BASE COUNT          735 a          449 c          395 g          529 t
ORIGIN      
        1 cgcagaagct ggagcagatt ttataaaggt aggcatagga ggaggctcta tctgcatcac
       61 aagagaacaa aaaggaatag gaagaggaca agcaacagcc ttgatagagg tagcacaagc
      121 acgagacgaa tactttgaac gcaccggcat ctatatccct atatgctcag acggaggaat
      181 agttcaggac taccacatgg tactcgcatt ggcaatggga gcagattttc tgatgatggg
      241 cagatacttt gcaagatttg acgaaagccc cacaaaaaaa cttatggtcg gaggcaccta
      301 tgtcaaggaa tattggggag aaggctccaa cagagcaagg aactggcagc gttacgacgc
      361 aggaggaaca gactcactta aatttgaaga aggcgtagac agctatgtac cttatgccgg
      421 caaactaaga gacaacctcg acataacact cggcaaagtc aaagccacaa tgtgcagctg
      481 cggagcatta accataaaag agcttaaaaa actggccaaa atcacactcg tctcatccac
      541 aagcatcatc gaaggaggaa ctcacgacgt catactcaaa gacacaaatt cttccggcat
      601 ataaaatgct gcttatgtca aactgccatt atgtcatagc atcacttcat ttgcaatcac
      661 ctgtaaatca atagttctta aaagctacat tgatatttgg catccatttt gaaattattt
      721 aagtcagaat ttaaaatgaa tgtctaactt aaaaaatgga ggatctggtt atgtcactag
      781 ttaaattttc aaatcaattc ccaacattgt tcgatagatt tttcgacaat gatctttttg
      841 actggtcaaa caagaatttc tcacttacca acacaactct tccatcagta aatattttgg
      901 aagacgacaa ttcatatgaa gtagaaatgg ctgcaccaag ccttaataaa gatgacttca
      961 aaattgagct gaacaatgat gtgctgacta tctcttctga aaagcaaact gaaaacgaaa
     1021 caaaagaggg gcagagatac acacgtaaag aattcagtta tcaatcattc agccgctcct
     1081 ttactttgcc tgattctgtt gacaacgaca aaatcaaagc aaaatatgaa aatggcgtcc
     1141 tgagagtcat aatacctaag agagaggatg caaagccaag accggttaaa caaattgcaa
     1201 tcgagtaatc gagtaattta tcagttctat gaaaaagtta gcactattat tggtcagctt
     1261 ttcaatagtt gtggccggct gctatggcca atcagacaaa aaaggccagc cggccacttc
     1321 tgttcaacct aaggctaaca taaaggtaaa caaggaatat gacaaaaacg gaagattaat
     1381 aaggtatgat tcaacctata cctatgtcta tcccggaacg gactcactct ttacagatga
     1441 gtttttcaaa gaatttgagc aaagattcaa ttcatacttt atttcacctc gcacatacat
     1501 gttcccggtt tacccgtttc cacaaatgag tcctttctac gaatttttcc agaacgactc
     1561 atctatggta aagtacaacc aatacagaaa acagatcgaa aagctgtata aggaaattga
     1621 ctcgataaga aacgcctttt actttgaaca gttcaacagt aagactaaga aaaagtaatt
     1681 ggaaattatt tgcttgttct tgttaaagag aatttaaaat cctgtcaaga gaatcataat
     1741 ctgagatcag cacctggcga gccttagaac cttcagaagg tccaacaata ccggccgcct
     1801 cgagctgatc cattattctt ccagccctgt tataacccag atttagtctg cgctgaatca
     1861 aggaagtaga accttgttga tactgtacaa tcagcctggc agcctcctca aacatcttgt
     1921 ccctcttcga aaggtcaacc tcaccaacac catatccatc attttcatca ccaacatatt
     1981 caggaagcat atgagcagta gcataaccct gctgactgct gataaaatcc acaatcctgt
     2041 caatctcctt ggtatcgata aaggcacact gaacccgtgt aatttccaca ttataaagga
     2101 aaagcatg
//
LOCUS       DAOTQL010000224         2107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_41651_length_2107_cov_4.193957, whole genome shotgun sequence.
ACCESSION   DAOTQL010000224 DAOTQL010000000
VERSION     DAOTQL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41651_length_2107_cov_4.193957"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="P5293_08925"
     CDS             complement(<1..339)
                     /locus_tag="P5293_08925"
                     /inference="COORDINATES: protein motif:HMM:NF037184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50056.1"
                     /translation="MILFFSKDQQIIIAVKIKEALSDDNLKALKWLLGNADEVPSELL
                     QGYFVGPRKEMVTPWSTTAVEITINMNIQGIERMEEFIRVDSPDAKHDKMLQRIYKDL
                     DEEIFTISREP"
     gene            415..1716
                     /locus_tag="P5293_08930"
     CDS             415..1716
                     /locus_tag="P5293_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRR50057.1"
                     /translation="MFDIDSYNREIENLFNQFPSYQRVGKEAYKPGLESMLRFDNALG
                     NPHKKFMSIHVAGTNGKGSVSHMIAAVIQKTGAKTGLYTSPHLTDFRERIRINGEMVS
                     REFVYDFLLKWKPFMEENKPSFFEITTAMAFDYFAKEGVDIAVIETGLGGRLDSTNII
                     TPILSVITNIALDHCEQLGNTLGEIAREKAGIIKHNVPVVVGEVLYSTRPVFVRKAEE
                     MESKIIFAQEYKFKDVKIADYNLDLKGEYQNVNIRTVLTALNLLSTNDKFQNLIHHNW
                     SDALIREALQNVAVSTGLRGRWEYLISNPPVLCDTGHNANGLEFVFAQLRSLKPKRLF
                     IILGFVADKKLDAIMGIMPHNAYYFLTQAKIPRALDANILAERVFKLGIKGEVKSSVE
                     EAIISFKKVQKDGDLLFIGGSNFIVAEAINFFENKPDFFAE"
     gene            1765..1839
                     /locus_tag="P5293_08935"
     tRNA            1765..1839
                     /locus_tag="P5293_08935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Val,seq:tac)
BASE COUNT          664 a          345 c          427 g          671 t
ORIGIN      
        1 aggttctctg ctaattgtaa aaatctcttc gtccagatct ttataaattc tttgcagcat
       61 tttgtcatgc tttgcgtcag gagaatctac ccttatgaat tcttccatcc tttcgatacc
      121 ttgaatattc atatttatgg tgatctcaac agctgttgta ctccatggag ttaccatctc
      181 ctttcttggt cccacaaaat aaccttgtaa cagttctgaa ggtacttcat cggcatttcc
      241 gagtaaccat ttcaatgctt ttaaattgtc atcagataaa gcttctttaa ttttgactgc
      301 aataataatc tgttgatctt tggaaaagaa tagaatcata aaaaaataat gtgatttgat
      361 tcgcaaaatt aattttttta agcgattaat tgccattttt gcaaaaaaca taagatgttc
      421 gatattgatt cttataacag ggaaatcgaa aatttattta atcaatttcc ttcttaccaa
      481 agggtgggca aggaggctta caaacctggt ctggaaagca tgttaaggtt tgacaatgct
      541 ttaggcaatc ctcataaaaa attcatgtca attcatgtgg cagggacaaa cggtaaaggt
      601 tctgtttcac atatgattgc ggctgttatt cagaaaacgg gagctaaaac tggactttat
      661 acatctcctc atttgactga tttcagagag agaataagga ttaacgggga aatggtttct
      721 cgtgaatttg tctatgattt tctgttgaag tggaagccat ttatggaaga aaacaaacca
      781 tcattctttg agatcacaac tgcaatggct tttgattact ttgcaaaaga aggggtggat
      841 attgcagtta ttgaaacagg acttggcgga agattggact ctacaaatat tattactcct
      901 attttgagcg taattacaaa tatagcactt gaccattgtg aacagcttgg taatactttg
      961 ggtgaaattg cgagggaaaa ggcggggatt attaagcata acgtccctgt agtagtcgga
     1021 gaggtgcttt attctacgag acctgttttt gtcagaaagg cagaggaaat ggaatctaaa
     1081 attatatttg ctcaagagta taaatttaag gatgtgaaga ttgcagatta taacctggat
     1141 ctcaagggag aatatcagaa tgttaatatc aggactgttc ttactgctct caatctgctt
     1201 tcgacaaatg acaagtttca aaatttaatt catcataatt ggagtgacgc tcttataaga
     1261 gaagcattgc aaaatgttgc cgtgagcact ggtttgcgtg gacgatggga gtatttgatt
     1321 agtaatccgc ctgttctttg tgatacaggc cataatgcaa acggtttaga atttgttttt
     1381 gcccaattgc gctctttgaa gccaaagcgt ctttttataa tacttggatt tgtggctgat
     1441 aagaagctgg atgctataat ggggattatg ccccataatg catactattt tttgactcag
     1501 gctaagattc caagggctct tgatgcaaat attcttgctg aaagagtttt taaacttggc
     1561 ataaagggag aagttaaatc ttcagtagag gaggcgataa tttccttcaa aaaggttcaa
     1621 aaggatggag atctgctgtt tataggcggt tcaaacttca ttgtggccga ggcaatcaat
     1681 ttctttgaaa acaaacctga tttttttgca gaataaaaaa atgaatctat atttgcagtc
     1741 ccaaacagaa cagtaagttc taaggggagc atagctcagt tggtttagag catctgcctt
     1801 acaagcagag ggtcgggggt tcgactccct ctgctcccac aaaagcggaa aaaacaagaa
     1861 ataaaggctt tatagcggtt aaaacaccaa aataaggcct tttttcatgc cacccaacct
     1921 cacttgcacc tgtcggagtt tgttgtaaat agctaataat taattttttg tcaatttggc
     1981 ttttaaaaaa ttcaaaaatt aaatcactgt taatgtgtta tttacaacaa actccgacag
     2041 gtgcgacagg tgattgagca aagagttttt aaagcaattc aagacagaag aagatgtaag
     2101 caagttt
//
LOCUS       DAOTQL010000225         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_41708_length_2105_cov_3.505854, whole genome shotgun sequence.
ACCESSION   DAOTQL010000225 DAOTQL010000000
VERSION     DAOTQL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41708_length_2105_cov_3.505854"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1583
                     /locus_tag="P5293_08940"
     CDS             165..1583
                     /locus_tag="P5293_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRR50058.1"
                     /translation="MIHSLLQLFGVTEDKLKELITISLAEGGDYSDLFFEHTLVDELI
                     LRDGEVNGAGSHIDYGMGIRVLAGDRTGYAYTEITSMKEMVNAAKSAYIIAKECKKSE
                     PAINVTPLENKNHYPIMVPWEESSITSRSIYIKELNDKIFSLDSRVNKVLGKLTISLT
                     EILFYNSEGILFTDERPMATLSVTVIMQQGSRIENGSSSRSFRMGAEFLTHQLIDEIA
                     KEVVEKTSILFDAEIPQGGEMPVVMGAGGSGILLHEAIGHSFEADFNRQNISIFSEKF
                     GKKICSEEISVVDDGTITSNRGAVNFDDEGVSGQKTYMVTQGYLTSYLHDRISAKFYN
                     TAPTGNGRRESFRFMPIPRMRATYMLNGKSKESDIIASVKKGIYVDTFSNGQVQIGAG
                     DFTFYVKSGNLIENGRLTKPIKDINIIGNGPMALADIVAVADNSIIDNGAWICGKEQY
                     CPVSCGMPSVLISKLIIGGVQQ"
     gene            1580..>2105
                     /locus_tag="P5293_08945"
     CDS             1580..>2105
                     /locus_tag="P5293_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HRR50059.1"
                     /translation="MKNHNISTAQFALETALEKGCNDARITLNESRQNTFTFRNDQLD
                     RLQQATSSSLFLQLYVDGKYGTFSTNKMDRKDLLDFISKGVEITKLITEDKDRGLPDK
                     NLYYRGGGKDLRQYDKLYSNISTEQKINIAKECNYEILGKDERILNVTSEFSDYEDYN
                     LIIDSQGFMGETEQT"
BASE COUNT          763 a          356 c          405 g          581 t
ORIGIN      
        1 ctactataga ttttacatca tcaagagaga tgtctccgtt tattatcaac actttagact
       61 ggtaatttta attcgacaaa tatagtttat attagaaaaa ttgccaaatt tataatttgt
      121 gatataatta atttttgggt tactttgcga aaaataaatt tttcatgatt catagcttat
      181 tgcaactttt tggagtcaca gaagataaac ttaaagaatt aataacaatt tcacttgccg
      241 aaggcggaga ttactcagac cttttttttg aacatacatt agtagacgaa ctcatattga
      301 gagacggtga agtcaatgga gccggttctc atatcgacta tggaatgggg ataagagtcc
      361 ttgctggtga caggactgga tatgcctaca cagaaataac ttcaatgaag gagatggtca
      421 atgctgcaaa aagtgcctat attatagcaa aagagtgtaa aaagtctgaa ccggccatta
      481 atgttacccc tctcgaaaac aaaaaccact atcctataat ggtaccttgg gaagaaagtt
      541 ccataacctc gagaagtata tatataaaag agttgaacga caaaatattt tctctggaca
      601 gcagagttaa taaagtatta ggcaaactga cgatatcgct tacagagata cttttttaca
      661 actccgaagg gatactcttt acagatgaaa gacccatggc aaccctttca gtaacagtaa
      721 taatgcagca gggatcaagg atagaaaatg gaagttcttc aagaagtttc agaatgggag
      781 ctgaatttct cactcatcag ctcatcgatg aaattgcaaa agaagtggtt gagaaaacct
      841 ccattctttt tgatgcagag ataccccaag gaggtgaaat gccggtagta atgggtgccg
      901 gaggctccgg catccttctc cacgaggcca taggacactc tttcgaagca gattttaacc
      961 gacaaaacat atcaatattt tcagaaaaat ttgggaaaaa aatttgttca gaagagatat
     1021 ctgtcgtcga tgatggaaca attacttcaa acagaggagc tgtcaatttt gatgacgaag
     1081 gtgtctccgg acaaaaaacc tatatggtaa cacagggata cttaacctca taccttcatg
     1141 accgaataag tgccaaattc tataataccg ctcccacagg caatggcaga agagaatcat
     1201 tccgttttat gcctattccg aggatgagag caacttatat gttaaatggc aaaagtaaag
     1261 aatcagatat aatcgcttca gtaaaaaaag gcatctacgt agatactttt tccaacggac
     1321 aggttcaaat aggagccgga gactttacat tttatgttaa gtcaggtaat cttatcgaaa
     1381 acggaagact cacaaagcca ataaaggata taaatattat tgggaatgga ccaatggctt
     1441 tggcagatat tgtagcagtt gccgataatt caattatcga caatggagca tggatatgcg
     1501 gtaaagaaca gtattgccct gtttcatgcg gaatgccttc tgtactgata agcaaattga
     1561 taataggagg agtacagcaa tgaaaaatca taatatttca acagcccaat ttgcattgga
     1621 aactgcttta gaaaaaggat gtaatgatgc cagaattact ttaaacgaat cacgtcaaaa
     1681 tacttttact tttaggaacg atcaactcga cagacttcaa caagccacaa gctcgtctct
     1741 ttttcttcaa ctttatgttg atggaaaata cggtaccttt tccactaata aaatggacag
     1801 gaaagacctt ctcgatttta tttctaaagg agtagaaatc actaaactaa taacagagga
     1861 caaagataga ggacttcctg acaagaattt gtactataga ggaggaggaa aagatttgag
     1921 acaatatgat aaattatatt ccaatattag cactgaacaa aaaattaata tagctaaaga
     1981 atgcaattat gaaattctcg gtaaagatga aagaatctta aatgtaacaa gcgaattctc
     2041 agattatgaa gactataacc ttatcataga ttctcagggt ttcatgggag aaactgaaca
     2101 gacaa
//
LOCUS       DAOTQL010000226         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_42434_length_2080_cov_4.434568, whole genome shotgun sequence.
ACCESSION   DAOTQL010000226 DAOTQL010000000
VERSION     DAOTQL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42434_length_2080_cov_4.434568"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="P5293_08950"
     CDS             complement(<1..1245)
                     /locus_tag="P5293_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50060.1"
                     /translation="MRKIFAISTILVFLCFSAILNGCVDLKTDNKPIPSSLGLIPSDF
                     DWKTVTEINCSIQVQSVSGISDAAPRTIKIFNSPLLNSSALIASGAAKPGFPYSVSLT
                     LPVAAKTIYIQEILPTGSSTVQSADVTSSSLNFTFTKSGMASFGAPLMVGDDPVPPVN
                     IPSNFDITLNASSSGTLSGFNSGESSAYGNKYKAYYLPAGVTNSNIKGINGWNNHLVL
                     YVAGKLTLSDFSINQGSLFILDGGIVEIPEFSLSAQTATDIPAIYIAPGGTLSLTSDK
                     KDSQTSGSSYIINRGKITSARNVTFSVSSTFYNVGTIDLSSTKPNFKPTLNFTNNSIL
                     YNSGTINTYTLDLNSAVTITNTSSGKISVKEYSQSNSNILDNYGEIAAEKTFTISSGP
                     KINNHCHITANAVSFYGTTVNMF"
     gene            complement(1394..>2080)
                     /locus_tag="P5293_08955"
     CDS             complement(1394..>2080)
                     /locus_tag="P5293_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 90"
                     /protein_id="HRR50061.1"
                     /translation="RYFKKNLIASFLFGDITEVPKIPSFVKSRPVEGENANSVLLPLD
                     KVRHFTTVNDRLKFVEKKDMLIGRAYVDQPHRIKFWEMYFNHPKCNLGNINPNLKDHE
                     EWRVKPISIDEHLKYKFILCIEGNDVASNLKWVMSSNSLAVMPKPTYETWFMEGKLIA
                     DYHYVEIKKDYSDLEEKLNYFIAHPQEAELISFHANEYVKKILDNKLEKLIALMVIKK
                     YFLSTGQIVK"
BASE COUNT          630 a          346 c          398 g          706 t
ORIGIN      
        1 aaacatgttc acagttgttc cgtaaaaaga taccgcattc gcagtgatgt gacaatggtt
       61 attaatttta ggaccggagg atatcgtaaa agttttttct gcagctatct ctccatagtt
      121 atctaaaata ttactgttgc tttgtgagta ttccttgact gagatctttc cactggaggt
      181 gttggtaata gtaactgctg agttaaggtc taaagtatat gtattgatgg taccactgtt
      241 atataatatt gagttgtttg taaaattgag tgtaggcttg aaatttggct tggtagagct
      301 aaggtctata gtgccaacat tgtaaaatgt tgatgacact gaaaaagtaa cgtttcttgc
      361 tgaggtgatc ttccccctat taataatgta ggatgagcca cttgtttgcg aatccttctt
      421 atcacttgtc aatgatagag ttccgccggg agcaatatat attgctggaa tatcagtagc
      481 agtttgtgca gaaagagaaa attcgggtat ttcaacaatg cctccatcca gaataaataa
      541 agaaccctga tttatactga aatccgataa tgtcagtttt cctgcgacat atagtacaag
      601 atgattattc cagccattta tgcccttgat attactattt gtaacgcccg cgggcagata
      661 gtaagctttg tatttgttgc catatgcact gctttctcca gaattaaatc cacttaaagt
      721 gccactagaa gacgcattga gtgttatgtc aaaatttgat ggaatgttta ccggagggac
      781 gggatcgtct ccaaccatca gaggagctcc aaaagaagcc atgcctgact ttgtaaatgt
      841 aaaattcaaa ctgcttgagg taacgtcagc gctttgaacc gtacttgaac ctgttgggag
      901 tatttcctgt atatagattg ttttggcggc aacaggcaaa gttaaactga ctgaataggg
      961 aaatcctggt tttgctgctc ccgaagctat taatgcagac gaattaagta gaggggagtt
     1021 aaaaatctta atagttcttg gtgcagcatc acttataccg gaaactgact gcacctgtat
     1081 ggaacagttt atttcagtta ctgttttcca atcaaaatct gagggtataa gacccaatga
     1141 agaaggaata ggtttattgt ctgttttaag atctacacaa ccgttcagaa tagcagaaaa
     1201 acataaaaaa actaatattg tggagattgc aaaaatcttt ctcatttaaa tgattttttt
     1261 tatttgtgcg aaaatatgaa atttatttaa ataaaaacaa gttttgttaa taaaattact
     1321 ttgaaatgtt tggatatttt aaagtaattt ttatatgaac atggtctaag ttgtgccagc
     1381 ctttagtttg ctattattta acaatttgcc ctgtcgaaag aaaatatttt tttattacca
     1441 tgagagcaat gagtttttca agcttattat ccaggatttt ctttacatat tcattggcat
     1501 gaaaggatat cagttctgct tcttgtggat gggcaataaa ataattcagc ttttcttcaa
     1561 ggtcagaata atcctttttt atctcgacat aatgatagtc cgctataagt tttccttcca
     1621 taaaccaggt ttcgtaagtt ggttttggca taacggcaag tgagttagac gacataaccc
     1681 atttaagatt tgaagccaca tcgtttcctt caatacataa aataaattta tacttcaggt
     1741 gttcatcaat agaaatcggt ttaactctcc actcttcatg atctttaaga ttggggttta
     1801 tgttgcccag attgcacttg ggatggttaa agtacatctc ccagaatttg atcctatgtg
     1861 gttgatcaac atatgcacgt ccaatcaaca tgtctttttt ttctacgaat ttcagtctgt
     1921 cgtttacagt tgtgaaatga cggactttat cgagtggcag taaaactgaa ttcgcatttt
     1981 ctccttcaac aggcctgctt tttacaaatg atgggatttt tggcacttca gttatatcac
     2041 cgaaaagaaa acttgctatc agattctttt taaaataacg
//
LOCUS       DAOTQL010000227         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_42571_length_2076_cov_5.057892, whole genome shotgun sequence.
ACCESSION   DAOTQL010000227 DAOTQL010000000
VERSION     DAOTQL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42571_length_2076_cov_5.057892"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="P5293_08960"
     CDS             complement(<1..1224)
                     /locus_tag="P5293_08960"
                     /inference="COORDINATES: protein motif:HMM:NF019902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50062.1"
                     /translation="MMNNDFRSIILDQRKDQKLAQWEGKCLDYLYIVKENPDVANFAP
                     GRIYNMIMRHGIEDVDNSLKIRGYEDLVRYKFFDGKIYGTLEALHDIMKFLKAAARRT
                     ETGKRILIMVGPVASGKSTIAALIKRGLERDKTPKYTIKGCPIHEEPLHLIPLDDRPR
                     WEQELGVKIEGTLCPVCQQMVEDNFMGEDGHIKWEDVPIVSIKFSEQKRVGIGTFQPS
                     DPKSQDISELIGRVNMSKMTRYGETDPRAYQFNGELQVANGGVIEYIELLKADVKFHY
                     VLITAAQEQMIKAPGFPQMYIDTLILAHTNQTEYDGFRGEKKNEALHDRMYPITVPWN
                     LRVDDEIKIYEKMIRESDFRGIHIAPNTLKVAAQFAILTRLVPSNKVSSIIQKMKIYN
                     GEITEELKKQEIDVKA"
     gene            complement(1214..1687)
                     /locus_tag="P5293_08965"
     CDS             complement(1214..1687)
                     /locus_tag="P5293_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50063.1"
                     /translation="MPELNRSNLEKILDVEVVEVQKEMDSAPTQEEPEQILRKNIIRA
                     NNILDSVETELKNGNFSARLVEVASLIINSVTQASTQIMINKSNLNSLQIKRDVLKLK
                     EKELEIKSRLLAGQQPVGRDRLVITDRETILKYLKDGQDVKQLETNKQMEANNDE"
     gene            complement(1808..>2076)
                     /locus_tag="P5293_08970"
     CDS             complement(1808..>2076)
                     /locus_tag="P5293_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50064.1"
                     /translation="KMGSLDGRITVFRDTFAASDYMTVGYKGPSEYDAGIIYLPYIQL
                     MVSKTIYEQSFHPTVGLMSRYAIHQHIFGSNLYYKKVDITGLPT"
BASE COUNT          562 a          457 c          352 g          705 t
ORIGIN      
        1 agctttgaca tcaatctcct gtttcttcag ctcctcagta atctcgccat tataaatttt
       61 cattttctga ataatggaag aaaccttatt tgaaggaaca agccttgtca gaatagcgaa
      121 ctgtgcagca actttcagag tatttggagc tatatggatt ccgcgaaaat cagattctcg
      181 aatcatcttc tcgtagattt ttatttcatc gtctacacga agattccaag gaacggtaat
      241 aggatacatt ctatcgtgta gtgcctcgtt cttcttctcc cccctgaaac catcatattc
      301 cgtttggttc gtgtgcgcca aaattaatgt atcaatatac atttgtggaa aaccgggcgc
      361 cttaatcatc tgctcctgtg cagcagtaat cagtacatag tggaatttca catcggcttt
      421 caacaattcg atgtattcaa taacaccacc attggctacc tgtaattcac cattgaactg
      481 atatgcacgg ggatctgttt caccgtatct tgtcatcttg gacatattga cacgaccaat
      541 caattcagaa atgtcctgcg atttgggatc cgatggctgg aatgtcccaa taccaactct
      601 cttctgttca gaaaacttta ttgacactat cggaacatct tcccatttaa tatggccatc
      661 ttctcccata aagttatctt caaccatttg ctgacaaacg gggcagaggg ttccttcaat
      721 cttcacaccc aattcctgtt cccaccttgg acggtcatcg agcggaataa ggtgtagagg
      781 ttcttcgtga ataggacatc ctttaattgt atatttgggt gtcttgtctc tttcaagacc
      841 tctcttgata agagcagcaa tcgtagactt accggaagct acagggccta ccataataag
      901 aatcctctta ccagtttcgg ttcttctggc ggccgccttg aggaatttca taatgtcgtg
      961 taatgcctca agagtaccgt aaattttacc atcaaaaaac ttgtatcgca ccaaatcttc
     1021 ataacctctg attttgaggg agttgtcaac atcttcaatt ccatgcctca taatcatatt
     1081 gtaaatcctg ccgggtgcaa aattggcaac atccggattc tcctttacga tgtaaaggta
     1141 gtccaaacat tttccttccc attgggcaag tttttgatct ttgcgctgat ccaatatgat
     1201 gcttcggaaa tcattattca tcattattcg cctccatttg tttatttgtt tctaattgtt
     1261 taacatcttg tccgtcttta agatacttga gaatagtttc tctatcggta ataactaatc
     1321 tatctcgacc cactggttgt tgaccggcga gtaatctact ttttatttca agttcctttt
     1381 cttttaattt taacacatct ctttttatct gtaaactatt taaatttgac ttatttatca
     1441 taatctgtgt tgatgcttga gttactgaat ttattatcag gctcgcaact tcaacaagtc
     1501 ttgcagagaa atttccattc ttcaattctg tttctactga atcaagaatg ttattcgccc
     1561 tgatgatatt ctttcttaaa atctgctcag gttcctcctg agtgggggca gaatccattt
     1621 ctttctggac ttccactacc tcgacatcaa gaattttctc aagattactt ctgtttaatt
     1681 ctggcatatt ccatacactc ctaataacat atatacttat tataccatat ttattaagaa
     1741 ttgtaaagaa taaaaaaacc ccctcctgta agggaggggg ttgattttac tctctaccta
     1801 atttacatta ggttggtagt cctgtgatat ccaccttctt gtagtagaga ttactaccaa
     1861 agatgtgctg atgaattgca tatctgctca tcaaacctac ggttggatgg aatgactgct
     1921 catagattgt ctttgatacc atcagctgaa tgtatggaag gtaaataata cctgcatcat
     1981 actccgatgg acctttatat ccaactgtca tataatctga tgcggcaaat gtatccctga
     2041 ataccgtgat acggccgtca agggaaccca tctttg
//
LOCUS       DAOTQL010000228         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_43562_length_2042_cov_3.082033, whole genome shotgun sequence.
ACCESSION   DAOTQL010000228 DAOTQL010000000
VERSION     DAOTQL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43562_length_2042_cov_3.082033"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..2013)
                     /locus_tag="P5293_08975"
     CDS             complement(22..2013)
                     /locus_tag="P5293_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRR50065.1"
                     /translation="MLIALVSGLSICSCKQSSEKKIQEPFKYLADEFADIKIIRFQIP
                     GWDSLSLQQKEYIYHLSEAVKCGRDIFWDQNSKYGLKVRKVIESILDNYNGDKECNDY
                     KEFIVYAKRVFFSNGIHHHYAEEKIIPGCSKEYFGELMSQSGLDTLKQEMLPVIFDLS
                     IAPYRKNISGKGDLIAESAVNFYEGVTHSEAESFYAKMEVPNDPRPISYGLNSRLVKK
                     NGILMEETCKIGGLYGEAIEKIVYHLEKAATVAENDIQKQYIKLLCDYYRSGDLKKWD
                     EYNIAWVKDTISRVDFINGFIETYNDPLGLKGTWEGMVNIKDIEASKRTQIISENAQW
                     FEDHSPIDPQYRKKNVKGVSAKVINAVQLGGDCYPVSPLGINLPNADWIRKEYGSKSV
                     TIANITEAYDKAAEESPKSMTAEFSWDDAEVQLIKQYGTLTGSLHTDLHECLGHGSGQ
                     LREGTSPNALKEFSSTLEESRADLFALYYLADPKLVELGVIPNDTAYKAEYINYIRNG
                     IFTQFVRIDLGKNVTQAHMQGRKMISEWCYAKGKSENVIEKRIRDGKTYFVINDYARL
                     RSLFEELLKELQRIKSEGDYEAGKKLVETYGIKIDPNLHKEVKERYAALNLKPYGGFI
                     NPEIIPVEKNGKVIDYKVEYPSDFLQQMIEYGKKYNFLN"
BASE COUNT          566 a          421 c          331 g          724 t
ORIGIN      
        1 gcaggttaat ttacgagtct tttagttcaa aaagttatat ttttttccat attctatcat
       61 ttgttgaaga aaatctgaag gatattcaac cttgtaatca atgaccttac catttttctc
      121 cacaggaata atttcaggat ttataaatcc tccgtaaggt ttaagattaa gagcagcata
      181 cctctctttg acctctttgt gaagattagg atcaatcttt ataccatagg tttctacaag
      241 tttttttcct gcctcgtagt ctccctctga tttgattctt tgcagttctt tcaaaagttc
      301 ttcaaaaagg gaacgaagtc tggcatagtc gtttatcaca aaataagttt taccgtctct
      361 aattcttttt tcaatcacat tttctgattt tccttttgca taacaccact ctgagatcat
      421 tttgcgcccc tgcatatgtg cttgagtgac gttttttcca aggtcgattc taacgaattg
      481 agtgaagatt ccattacgaa tatagttgat atattcggcc ttgtaggcag tatcatttgg
      541 tattactcca agctcgacaa gtttggggtc tgcaagataa taaagcgcga ataaatctgc
      601 tctggactct tctagagttg aactgaattc tttcaaggca tttggtgatg tgccttctct
      661 aagttggcct gatccatgcc caagacattc atgaagatct gtatgtaaac tgcctgtcag
      721 agttccatac tgtttgatta gttgtacctc tgcatcatcc catgaaaact cggccgtcat
      781 actctttgga gactcttctg cggccttgtc atatgcttca gtaatattgg caattgtgac
      841 agattttgag ccatactctt tgcgaatcca atcggcattc ggcaaattaa tcccaagagg
      901 tgacacagga taacagtctc cacctagttg tactgcattg ataacttttg cggacacacc
      961 cttgacattc ttttttctgt attgtgggtc tataggtgaa tgatcttcaa accattgagc
     1021 attttcactt attatctgtg tgcgttttga ggcttcaata tccttaatat ttaccattcc
     1081 ttcccaagtg cctttaagcc caagaggatc attataagtt tcaataaaac cattaataaa
     1141 atctacccgg gagattgtat ccttaaccca tgctatatta tattcgtccc attttttaag
     1201 atccccgctt ctataatagt cacagagaag cttaatatat tgtttctgga tatcgttctc
     1261 tgccactgtt gccgcctttt caagatgata gactattttt tcgatcgctt ctccataaag
     1321 acctccgatt ttacaagtct cttccatcag gatcccgttc ttcttgacaa gtctggaatt
     1381 aagtccatat gatataggtc ttggatcatt aggtacttcc atttttgcat aaaaggattc
     1441 agcctctgag tgtgtaacac cttcataaaa attgactgca ctttctgcaa tcagatcacc
     1501 ttttccagat atgttttttc tatatggggc aatggataga tcaaaaatga caggaagcat
     1561 ttcttgcttc agggtatcca aaccgctctg cgacatcagt tctccgaaat attccttaga
     1621 acaaccggga ataattttct cctctgcata atgatgatga ataccgttgc taaaaaacac
     1681 tctcttggca tatactataa attctttgta atcgttacat tccttgtctc cattgtaatt
     1741 gtcaagtata gattctatga cttttcttac cttaagtcca tattttgaat tttgatccca
     1801 aaatatatcc cttccacatt taacagcttc acttaaatga tagatatatt ctttctgctg
     1861 aagagacagt gaatcccatc ctggaatctg aaaacggatt attttaatat cagcaaattc
     1921 gtctgccaaa tatttgaacg gttcctgaat tttcttttca gatgactgtt tgcatgagca
     1981 aattgaaagc ccggaaacta aagcaataag cataatttta tatcttttca taaatctaaa
     2041 tt
//
LOCUS       DAOTQL010000229         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_44020_length_2028_cov_3.737456, whole genome shotgun sequence.
ACCESSION   DAOTQL010000229 DAOTQL010000000
VERSION     DAOTQL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44020_length_2028_cov_3.737456"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="P5293_08980"
     CDS             <1..1449
                     /locus_tag="P5293_08980"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRR50066.1"
                     /translation="KARYTINGQTKLHENGLVFVNTSITDRYGSKGVINGGITYTHLR
                     NLGFNTTIHFSNIELINLTESDNSTFYGKAFGNGYFNLRGNLKKIFMELSAQSNKNTE
                     IHIPLSNGTEAVNSNLLKFVQNTSFTADKITIHQTQEKKEKPASTELEVQLKLNITPD
                     AAMLIEIDKSAGDVIKGFGSGLISMDIKPSKHIFNIQGDYLIESGSYKFVLQGIFNRD
                     FIIQQGGTIGFNGDINKTTLNLTAKYRTKTSINTLISDTSSVSNRRTVDCLIKLTGPL
                     MNPNIAFDIEIPDLDPITKARVDAALNTEDKVIKQVMSLLVSNSFIPDIQSSVVNNST
                     LLYSNATEVLSNQINKIFNQLDIPLDLSFNYQPGQNGRDIFDAAVSTQLFNNRVIING
                     NIGNARYSNSTNEVVGDVDVEIKLDEKGKFRAKLFSHSADQFSNYLDNSQRNGGGFVY
                     QEEFTTFKELLKSIFGNKKKRKAENKANNIKE"
     gene            complement(1439..>2028)
                     /locus_tag="P5293_08985"
     CDS             complement(1439..>2028)
                     /locus_tag="P5293_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR50067.1"
                     /translation="IERYRISSIEPNLLTEDIIKWVAENSKFLHHFHIPLQSGSDKIL
                     ALMRRRYNTSLFREKVNLVRRYMPDAFIGVDVIVGFPGEGVSEFNESFNFLKTLRPAF
                     IHVFPYSKRANTLAANMDEQVDEKNKHERVIRLESLCKILHDEFVDSQKGKKEYVLFE
                     STQKGGKMYGYTRNYIRVERPYDRYAIGKIVEVTL"
BASE COUNT          714 a          390 c          335 g          589 t
ORIGIN      
        1 aaagcaagat atactataaa tgggcaaaca aaacttcacg aaaacggact tgtctttgta
       61 aatacatcta ttacagaccg atatggatca aaaggtgtca ttaatggtgg aattacatat
      121 acacacctcc gtaatcttgg tttcaatact actattcatt tttccaatat agaacttatc
      181 aatctcactg agtcagataa cagcacattt tatggaaaag cctttggtaa tggttatttc
      241 aatctaagag gcaatctgaa aaagatattt atggagctat cagctcagtc caataaaaac
      301 actgagatac atattcctct ttcaaacggt actgaagccg taaattccaa tttattgaaa
      361 tttgttcaga atacaagttt tacagcagat aaaatcacaa ttcaccaaac tcaagaaaaa
      421 aaagaaaaac cagcatctac agagctcgag gtccaactta aattgaatat cacccctgat
      481 gctgcaatgc tcatagaaat tgacaagtct gcgggtgatg tcatcaaagg atttggaagc
      541 ggactaatta gtatggatat caaaccttca aaacacattt ttaacattca aggagactac
      601 ttaattgaaa gtggttcata taaatttgtt ttgcaaggca tatttaacag agattttatc
      661 attcagcaag ggggtactat aggttttaat ggagatatta ataaaacgac tctcaatctt
      721 acggcaaaat acagaactaa gacatcaatc aacactttaa tttcagatac cagctcagtc
      781 agtaacagaa gaaccgtaga ctgtctgatt aagttaacag gacctttgat gaaccctaat
      841 atcgcctttg acatagaaat acccgatctg gatcccatta cgaaagcaag agttgacgca
      901 gcacttaata ccgaagataa agttattaaa caggtaatgt ctctcctcgt ttccaacagt
      961 tttattccgg atatccagtc aagtgttgta aataattcca ctctgttgta ttccaatgcc
     1021 acagaagtgt tatccaatca gataaacaaa attttcaatc aattggacat cccacttgac
     1081 ctgagtttca attatcagcc tgggcaaaac ggcagagata ttttcgacgc cgctgtgtca
     1141 acacagcttt tcaataacag agtcataatc aacggcaaca taggaaatgc aagatattca
     1201 aacagtacaa atgaagttgt gggcgatgtg gacgttgaaa taaaactgga tgaaaaggga
     1261 aagttccgtg ccaagttgtt ctcacattct gcagatcaat tttcaaatta tttggacaac
     1321 agccagagaa atggtggcgg tttcgtttat caggaagagt ttacaacatt caaggaattg
     1381 ctaaaaagca tattcggaaa caagaagaaa cgtaaggccg agaacaaagc caataatatc
     1441 aaagagtaac ttcaacaatt tttcctattg cataacggtc ataaggccgt tcaaccctta
     1501 tataatttct tgtgtagcca tacattttac cacccttttg agtactctca aaaagtacat
     1561 attctttttt acccttttga gaatccacaa attcatcatg caatattttg catagagatt
     1621 caagtcttat aacacgttca tgtttatttt tttcatccac ctgctcatcc atattggctg
     1681 caagagtatt tgctcttttg gagtatggaa aaacatgaat aaatgctggt cgtagagttt
     1741 ttagaaaatt aaaactctca ttaaattcgc tcacaccctc acctggaaac cccacaatca
     1801 cgtcgacacc tataaaagcg tcaggcatat atcttctcac aagattaacc ttctctctaa
     1861 aaagagaagt attataacgt cgacgcatta acgcaagaat tttgtctgaa cccgattgta
     1921 gtggaatatg aaaatgatga agaaatttag aattttctgc cacccattta attatatcct
     1981 cagttagcaa atttggctct atagaagata tccggtaacg ttctatac
//
LOCUS       DAOTQL010000230         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_46160_length_1964_cov_4.627554, whole genome shotgun sequence.
ACCESSION   DAOTQL010000230 DAOTQL010000000
VERSION     DAOTQL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46160_length_1964_cov_4.627554"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..876)
                     /locus_tag="P5293_08990"
     CDS             complement(121..876)
                     /locus_tag="P5293_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HRR50068.1"
                     /translation="MKPQVLVMNFTHVHEHEKSLQNCEYKWIDCTKLSGTSRYCDNEA
                     EKQLIEKIAPYTPEGIHFIDSGNYHYMSKLWIDKITRPFYLIVFDHHPDMQPALFDNL
                     LSCGCWVKKLLDTNHYLKEVYILGVSEEYINNIEPEYKDRLVVYSDKDLNRKDTLLHI
                     ADLKLDAPVYISIDKDVLDTNCAATNWNQGSLSLTKLRDMLSNLAKINNVIGVDICGE
                     CSTNLDLYNLRKCTAINNFANKVLIDTLSDHMR"
     gene            complement(1002..1319)
                     /locus_tag="P5293_08995"
     CDS             complement(1002..1319)
                     /locus_tag="P5293_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50069.1"
                     /translation="MNTVIYYSTGDNKVHYYNPSNQTEKRDLITLPAGEEIVCIEQSY
                     DYYYKVNSFAVLSNLNGNWILRTYDMVGSTPDVALPEKAIYHGTGIAKNFLYRHANTS
                     LTY"
BASE COUNT          585 a          344 c          344 g          691 t
ORIGIN      
        1 tcggaaccga accgagaatg tcaattacag cgaacattac aataaatgca ctgaagatct
       61 ccctgaaatt tatacccatc tttattattg ttataatgtt ggctgtaaag ataataaaaa
      121 ttatctcata tggtcggaaa gagtgtctat aagcactttg tttgcaaaat tatttatagc
      181 cgtacatttt cgaagattgt aaaggtcaag gtttgtagaa cattcgccac atatatctac
      241 tcctatgaca ttattaatct tggcaagatt tgagagcata tccctaagtt ttgtcaaaga
      301 gagtgatccc tggttccaat tggtcgcagc acagtttgtg tccagaacat ccttgtctat
      361 agatatgtat acaggtgcat caagtttgag gtctgcaata tggagcaaag tatcttttct
      421 gtttaaatct ttatcgctgt agacaaccaa tctatcttta tattcgggtt ctatattgtt
      481 aatgtattct tctgatacgc cgagaatata aacttctttg aggtaatggt tagtgtcaag
      541 aagtttttta acccagcagc cacaggatag taaattatca aacaaagcag gttgcatgtc
      601 agggtgatga tcgaaaacaa tcaaatagaa aggtctggtt atcttatcaa tccatagttt
      661 actcatatag tgataatttc ctgaatctat aaaatggatt ccctcagggg tgtagggagc
      721 aattttctca attagttgtt tttctgcttc attgtcgcaa taacgactgg tgccagataa
      781 ttttgtgcag tcaatccact tatattcaca attttgtagg cttttctcat gctcatgaac
      841 atgagtgaag ttcataacca aaacttgggg tttcatacac agtatatatt tttcccaagt
      901 tatgaaatta ttttaactca gtggctgcca aaagaaatta aatggcatat tgttcttccg
      961 tacaattgat attgtttctt ttggcagcca ctgagttagg tttaataggt caaagaggta
     1021 ttggcgtgtc tgtataaaaa gttctttgct attccggtac catgataaat tgctttttca
     1081 ggaagagcaa catcaggtgt agagccaacc atatcatagg ttctcaatat ccagtttcca
     1141 ttgagatttg agaggacagc aaaagagttg actttgtaat aatagtcata tgattgctcg
     1201 atacatacaa tttcttcgcc agcaggcaga gttattaaat ctctcttttc tgtctgattg
     1261 cttgggttgt agtaatgtac cttgttatca ccggtagagt agtatataac tgtattcaat
     1321 gctgtacttg ctccaccttt caaagcaaat acttttgcgt ttaaaactcc aaatgtttaa
     1381 ggaataacaa gtccctcggc tccattatca tgaatgtagc catattatat gttgccaata
     1441 ccgatttgcc acaagtgaga cacctttgta tttctgtttt ttacaatcac atagctgcta
     1501 cctaaggtat agtttttcat attgtcattt gagtatgggt atccaaattg tccaacattg
     1561 gcgccattat tatttacgaa gctcacagca ttattattta gtaaaacaaa gccgttggta
     1621 agagcccaaa ctctttgaga taggatgcaa caagtttatt gtttcatcct cctgcttgat
     1681 atctttgttg aagtttgcca aatgcaagat cagggttaaa agcttcttct actttatcta
     1741 taaaacttaa gtttaatact cctccggcaa acatatgaat caagttgcct tcattgtcga
     1801 gaattacaaa tgtaggatat gcatgtatgc cgtatttgtt tttaaaggct ggaccctctt
     1861 ctcctttctc tgcatttaat ttgatgctta cacatatttt ggattaaaat aatctcccat
     1921 taaaattatc aaaatctttt tcattttttg atatttgttt atat
//
LOCUS       DAOTQL010000231         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_46164_length_1964_cov_3.970665, whole genome shotgun sequence.
ACCESSION   DAOTQL010000231 DAOTQL010000000
VERSION     DAOTQL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46164_length_1964_cov_3.970665"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="P5293_09000"
     CDS             <1..926
                     /locus_tag="P5293_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HRR50070.1"
                     /translation="KPMAIFFSIFGLIGTLCLMQANQLTEALFATFVVPNGYKSTFGL
                     KLGIGIFICVIVSIVVLGGIERISKVSAKLVPTMIALYFILVGYIIVTNLPLIPDILS
                     DIFTQAFQFKAGLGGLAGSAIVIGARRAVFVNEAGVGTASMMHGASKNSEPVREGLVA
                     MIAPSIDSGLVCTLTAFAILINAQYEVSQIQGIQMAINSFEASIPGLGSYFLMLISII
                     FAFSTMFSYSYYGVKCTNFLFGAKYAHYYNYFFLIMLIVGAVISVDVVVGVIDSAYAL
                     MAIPTMTCLILLAPNVKREMKKYFAANKKIL"
     gene            923..>1964
                     /gene="argS"
                     /locus_tag="P5293_09005"
     CDS             923..>1964
                     /gene="argS"
                     /locus_tag="P5293_09005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRR50071.1"
                     /translation="MNPEELIKEKVNEAISELYGVNAGKDIIQIQATRKEFEGDLTAV
                     IFPLVKYSKKTPEITGEEVGSYIKEHSSEIASFNVVKGFLNIKLSGRYWYNIFKEIYG
                     NEDFGQAKPTGKNIMVEFSSPNTNKPLHLGHIRNNLLGWSLSKLLEANGHNVIKVNLV
                     NDRGIHICKSMIAWLREAKGETPETTGIKGDHLVGKYYVAFNDEYKKEVDELVAKGIP
                     KEDAEKNAPIMREAQSMLVKWEKGDKEVRDLWSTMNSWVYKGFDLTYKRLGIKFDRVY
                     YESQTYLLGKALVKIGLEKGVFFEKEDGSVWCDLTSDGLDEKLLLRSDGTSVYITQDL
                     GTAHQRFSEYNLD"
BASE COUNT          614 a          277 c          424 g          649 t
ORIGIN      
        1 ggaaaccaat ggccattttc ttttctattt ttggtttaat aggtactctt tgtcttatgc
       61 aggcaaacca acttacggag gcactgtttg caacatttgt agtgccaaat ggttacaaaa
      121 gtacatttgg ccttaaattg gggatcggga tttttatatg tgttattgtc tcgattgttg
      181 ttttaggtgg aatcgagcgt atttccaaag tttcagccaa acttgtccca actatgatag
      241 ctctttattt tattcttgta gggtatataa ttgtaactaa tcttcctctt atcccagata
      301 ttttgagtga tatatttact caggcatttc agttcaaagc aggtttagga ggactggccg
      361 gctctgctat cgtgataggt gcaaggcgag cagtttttgt aaatgaggca ggggtgggta
      421 ctgcctcaat gatgcacggg gcttctaaaa acagtgaacc tgttagagaa ggtcttgttg
      481 ccatgattgc tccttcaatt gattcaggat tagtttgtac cttgacggca tttgctattt
      541 taataaatgc tcagtatgaa gtatcacaaa ttcaggggat acaaatggct ataaattcgt
      601 ttgaagcttc aattccagga cttgggagtt attttttgat gcttatatct attatttttg
      661 cgttttcaac aatgttttcc tactcatact atggagtaaa atgtactaac ttcttatttg
      721 gagccaaata tgctcattat tataattatt tctttcttat aatgttgata gtaggtgctg
      781 taatatctgt tgatgtggtg gttggagtga ttgacagtgc atatgccctt atggctattc
      841 ctacgatgac ttgtcttatt ttgcttgctc ctaatgtaaa gagagaaatg aaaaaatatt
      901 ttgctgctaa caaaaaaata ctatgaatcc tgaagagctt ataaaagaaa aagtaaatga
      961 agcaatttct gaattgtacg gagttaatgc tggaaaagat attattcaga tacaagctac
     1021 aagaaaagag tttgaaggag atttaacagc cgttatattt ccattagtga aatattccaa
     1081 aaaaacacct gaaattaccg gagaagaagt cggatcttat attaaggagc actcttctga
     1141 aattgcctca tttaatgtag ttaaaggctt tttgaacata aaactttcgg gcagatactg
     1201 gtataatata ttcaaggaaa tatatggtaa tgaagatttt ggtcaggcaa aacctacagg
     1261 taaaaatatt atggtagaat tttcctctcc aaatacaaac aaacctcttc atctgggtca
     1321 tataaggaat aatttgcttg gttggtctct ttcaaagttg ctagaggcaa acggtcataa
     1381 tgttataaag gttaatttgg tgaatgacag gggaattcat atttgcaagt caatgattgc
     1441 ttggttgaga gaagcaaaag gagaaacccc tgaaactacg ggcattaaag gagaccatct
     1501 tgttggcaaa tattatgtgg catttaatga cgaatacaaa aaggaagtgg atgaattggt
     1561 agcaaaaggt atcccgaagg aagatgccga gaaaaatgct ccaataatga gagaggctca
     1621 gtcaatgctt gttaaatggg aaaaaggaga taaagaggta agagatttgt ggtctacaat
     1681 gaattcttgg gtatataaag gatttgattt gacttataag aggttaggga ttaaatttga
     1741 cagggtttat tatgagtctc agacatatct tcttggtaaa gctcttgtta agattggtct
     1801 tgagaaaggt gttttttttg aaaaagagga tggatctgta tggtgtgacc ttacttcaga
     1861 tggtcttgat gagaaactgc ttctcagatc agatggaacg tctgtatata taacacagga
     1921 tttgggaaca gctcatcaaa gattttctga atataatctt gatg
//
LOCUS       DAOTQL010000232         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_46281_length_1961_cov_5.043022, whole genome shotgun sequence.
ACCESSION   DAOTQL010000232 DAOTQL010000000
VERSION     DAOTQL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46281_length_1961_cov_5.043022"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /gene="rpsC"
                     /locus_tag="P5293_09010"
     CDS             complement(<1..522)
                     /gene="rpsC"
                     /locus_tag="P5293_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRR50072.1"
                     /translation="MGQKTNPISNRLGIIRGWDSNWYGGTDYAGKLLEDAKIREYLNT
                     RLSKASLSRIVIERTLKLITVTIHTARPGIIIGKGGQEVDKLKEELKKISNKEVQINI
                     FEVKRPEIDANIVANNIARQVEGRVSYRRAIKMAMASTMRMGAEGIKVLISGRLGGAE
                     MARRELYKEGRTPL"
     gene            complement(524..946)
                     /gene="rplV"
                     /locus_tag="P5293_09015"
     CDS             complement(524..946)
                     /gene="rplV"
                     /locus_tag="P5293_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRR50073.1"
                     /translation="MGARKRESAERRKEIKRQTAIAKLNNVPTSPRKMRLVADNIRGL
                     EVNHALDVLKYTKREAAVRLEKFLRSAIANWEAKNESDRKELESGNVIVKTIMVDPGR
                     SLKRIRTAPQGRAARIRKRSNHVTIVLDKKSKTVKEEQ"
     gene            complement(976..1239)
                     /gene="rpsS"
                     /locus_tag="P5293_09020"
     CDS             complement(976..1239)
                     /gene="rpsS"
                     /locus_tag="P5293_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRR50074.1"
                     /translation="MSRSLKKGPYIEASLEKRVLAMNESGKKEVIKTWSRASMISPDF
                     VSHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHSGNR"
     gene            complement(1258..>1961)
                     /gene="rplB"
                     /locus_tag="P5293_09025"
     CDS             complement(1258..>1961)
                     /gene="rplB"
                     /locus_tag="P5293_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRR50075.1"
                     /translation="GRNAEGKMTMRYIGGGHKRMYRVIDFLRNKDNYTATVKTIEYDP
                     NRSARIALVVYGDGEKRYIIAPHGLKVGQVIASGPGVAPELGNALPLSEIPLGTLIHN
                     IELHPGQGAAMARSAGTYAQLTAREGKHAILRLPSGETRMVPVTCRATVGTVSNPDHN
                     LESDGKAGRQRWLGRRPRTRGVAMNPVDHPMGGGEGKASGGHPRSRKGLLAKGYKTRN
                     PKKTTKKFIIEKRKK"
BASE COUNT          466 a          446 c          373 g          676 t
ORIGIN      
        1 cagaggagtt ctgccctctt tgtagagttc tcttcttgcc atttcggctc ctccaagacg
       61 accgcttatc aaaactttta tgccttctgc tcccattctc atagttgagg ccattgccat
      121 cttgatagca cggcggtagg atacacgacc ctcaacttga cgtgcaatat tgttggcaac
      181 tatattggca tcaatctcag gacgtttaac ttcaaagata ttgatttgta cctctttgtt
      241 ggagattttt ttcaactcct cttttagttt gtcaacttct tgtccgcctt tgccaataat
      301 aatccctggt cgtgctgtgt gtatagtaac tgttatcagt tttaaagttc tctcaataac
      361 aattcttgaa agacttgcct ttgataatcg ggtatttaag tactctctta tttttgcgtc
      421 ttctaataat ttgccggcat aatcagtacc accgtaccag ttagagtccc agccacggat
      481 aatgccaagt ctgtttgata taggatttgt tttttgtccc atcttattgc tcctctttta
      541 cggttttact ttttttgtca agcactatag taacgtggtt ggaacgtttg cgaattctgg
      601 ctgcacgacc ttgaggggca gtacgaatac gcttgagtga gcgacccgga tcaaccataa
      661 ttgtcttaac gattacattg ccactttcca attctttacg atctgattcg tttttagctt
      721 cccaattagc aattgctgaa cgaagaaact tctccaaacg aacagctgct tctcttttgg
      781 tatattttaa tacatccaga gcgtggttaa cttcaagtcc tcttatatta tctgcaacta
      841 aacgcatctt gcgaggtgaa gtcgggacat tattcagctt ggcaatagct gtttgtctct
      901 ttatttcttt acgcctttcg gcagactctc tttttcgagc tcccattatt taattctgtt
      961 ttttcaaatt aatgattaac gattacccga gtgacctcta aaggtgcgtg tgggtgcaaa
     1021 ttcaccgagt ttgtgaccta ccatattctc agtaatgtaa acaggaataa acttgtttcc
     1081 attatgaaca gctatagtgt gacttacaaa atcgggcgat atcatacttg cgcgtgacca
     1141 agttttaatt acctctttct tgccagactc attcatggca agtactcttt tttcgagact
     1201 agcctcaatg taaggccctt tttttaatga acgactcata atattcagtt aattccgtta
     1261 ttttttcctt ttttcaataa taaatttctt agtcgtcttt ttaggattgc gggtcttata
     1321 accttttgca agcaagcctt ttcttgaacg agggtgtcca ccggatgctt taccttcacc
     1381 acctcccata gggtgatcaa cagggttcat tgcaacacct cgagtacgag gtctgcgtcc
     1441 tagccaacgt tgacgtccgg cttttccgtc agactccagg ttatggtcag gattggacac
     1501 tgtaccaaca gtagcacgac atgttacagg aaccattctg gtctctcctg atgggagtct
     1561 cagaatagca tgttttcctt cacgagctgt aagctgagca tatgttccgg cgcttcgtgc
     1621 cattgcagca ccttgtcctg gatgtaactc aatgttgtga ataagtgttc caagtggaat
     1681 ttctgataat gggagagcgt ttcctaattc cggagcaact ccagggccgg aggcaattac
     1741 ttgtccgact ttaagcccgt gtggagctat aatatagcgt ttctctccat ctccataaac
     1801 aactaatgca attcgggcac ttctgttagg atcatactcg attgtcttta ctgttgcagt
     1861 gtaattatct ttatttcgta aaaagtcgat gacgcggtac attcttttgt gaccgcctcc
     1921 aatatagcgc attgtcatct taccttcagc atttcttcct c
//
LOCUS       DAOTQL010000233         1951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_46628_length_1951_cov_3.197785, whole genome shotgun sequence.
ACCESSION   DAOTQL010000233 DAOTQL010000000
VERSION     DAOTQL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46628_length_1951_cov_3.197785"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="P5293_09030"
     CDS             complement(<1..1829)
                     /locus_tag="P5293_09030"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRR50076.1"
                     /translation="MSDIKRVYLFGGKTAEGDGSMKNLLGGKGANLAEMCKLGMPVPA
                     GFTITTETCAEYYKNNMQFPEGLKEEVDKALAKTEAIMNMRFGDKDNPLLVSCRSGAR
                     SSMPGMMETVLNIGLCSETIPGLIKKTQNPRFVYDAYRRLIMMYSDVVMEKAENIEPA
                     EGMGIRVQLDRMLNELKQKKGYKSDTDLTTEDLIQLCEDFKNKIIEVLGKPFPDNAYE
                     QLWGGIGAVFKSWNGKRAIAYRRIEGIPDEWGTAVTVQSMVFGNMGDKSATGVAFSRN
                     PATGEAKFYGEWLINAQGEDVVAGIRTPNPLNEATKNEHNRHLSSLETAMPSVYAELF
                     EIQKKLEQHFHDMQDIEFTIQDGKLWMLQCRVGKRTGLAALNMAMDMLQEGLIDEKTA
                     VMRVAPVQLDELLHPILDPAAEKVANVVAKGLPAGPGGSVGEIVFTAETAVEAAKKGK
                     KVILVREETNPEDVEGMRAADGILTARGGMTSHAALVARGWGKCCIVGCDSLHIDLHS
                     KKMTINGKTYSEGSVFSLNGSKGFVYDTAISTVNASENPRFVEYMKIVDKYRVLGVRT
                     NADTPEDAQNAIDFGAEGIGLFRIEHMFYGKNSEQPLAKLRKM"
BASE COUNT          507 a          435 c          356 g          653 t
ORIGIN      
        1 atcattttac gaagcttagc caaaggttgt tctgaatttt tgccataaaa catatgttca
       61 atcctgaata gcccaatacc ttctgcgcca aaatcaatag cattttgtgc atcttctggt
      121 gtgtctgcgt ttgtacggac cccaagaact ctatatttgt ctacaatctt catgtattcg
      181 acaaaacgag ggttttctga agcgtttacg gttgaaatag cagtgtcata aacaaaacct
      241 ttactcccat tcaaactgaa aacactgcct tcgctatatg ttttaccatt aatggtcatt
      301 ttttttgaat gtaaatctat atgaagtgaa tcgcaaccca caatgcaaca tttaccccat
      361 cctcttgcaa caagagctgc atgagatgtc attcctcctc ttgctgtaag aataccatca
      421 gctgcacgca tgccttcaac atcttctgga ttggtttctt cacgtacaag aataactttt
      481 ttaccttttt ttgcagcttc aactgcggtt tctgccgtga agacaatttc ccctactgag
      541 ccgccaggac ctgcaggcag tcctttggct acaacatttg caaccttttc ggctgcggga
      601 tccaaaatag gatgaagaag ttcgtcaagt tgtactggag ctactctcat gacagctgtc
      661 ttctcgtcta tcaatccttc ttggagcata tccatggcca tgttcagagc tgccaagcct
      721 gttcttttgc caacgcggca ttgaagcatc cacaattttc cgtcttgaat tgtgaattcg
      781 atgtcctgca tgtcgtggaa atgttgttca agtttcttct ggatttcgaa aagttctgca
      841 taaactgatg gcatggctgt ttcaagagat gacagatgtc tgttatgctc atttttggta
      901 gcttcattaa gaggattagg tgtgcggata cctgctacga catcttctcc ttgagcattg
      961 ataagccatt ctccgtaaaa ctttgcctct cctgtagccg ggtttcttga gaaagcaaca
     1021 ccagtagcag atttgtctcc catattgcca aaaaccatcg attgtacagt tactgcagta
     1081 ccccattcat caggaattcc ttcaattctt ctatatgcta tggcacgttt tccgttccat
     1141 gatttgaaaa ctgcaccaat tcctccccac aattgttcat atgcattgtc aggaaaaggt
     1201 tttcctaata cttcaattat tttattttta aaatcttcac ataattgtat aagatcttca
     1261 gtagttagat cagtatcact cttgtagcct tttttctgct tgagttcatt gagcattctg
     1321 tcaagttgga cgcgtatgcc cattccttca gcaggttcga tgttttctgc cttttccatt
     1381 actacatcag aatacatcat tatcaaacga cgatatgcgt cgtaaacaaa acgtggattt
     1441 tgagtttttt ttataagtcc tgggatggtt tcggagcaaa ggccaatatt cagtactgtt
     1501 tccatcatgc cgggcatgga gcttcttgca cctgaacgac atgaaacaag aagaggatta
     1561 tctttatctc caaatctcat gttcattatt gcttcagttt ttgcaagagc tttatcaact
     1621 tcttctttga gcccttcggg aaattgcatg ttgtttttgt agtactcagc acaggtctct
     1681 gttgttattg taaagcctgc aggtacaggc atcccaagtt tgcacatctc agccaggttg
     1741 gcgcccttgc ctcccaggag atttttcatt gaaccgtcac cctcggctgt cttaccgccg
     1801 aaaaggtaaa ctcttttaat atcagacatt gtgataaaat aggttatgtg ttaaaaataa
     1861 ttggtttctt ttaaaaaatg agggggcaaa tataataatt ttataataat tttccggcca
     1921 actctgaaag tgcacttctt tcacctttga c
//
LOCUS       DAOTQL010000234         1944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_46870_length_1944_cov_3.191106, whole genome shotgun sequence.
ACCESSION   DAOTQL010000234 DAOTQL010000000
VERSION     DAOTQL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46870_length_1944_cov_3.191106"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..620
                     /locus_tag="P5293_09035"
     CDS             141..620
                     /locus_tag="P5293_09035"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRR50077.1"
                     /translation="MNNKSITIPYIECGFGEELDEKDNELLNAAKEATKLSYAPYSGF
                     CVGAALRLANGEIISASNQENAAYPSGLCAERVAIFYANSKYPDVAVESLAVTAAVDG
                     KICEMPTYPCGACRQVIAESEKRFGKRIRIIAGGEKIIQIIDGINGLLPFAFDNLPR"
     gene            658..757
                     /gene="ffs"
                     /locus_tag="P5293_09040"
     ncRNA           658..757
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5293_09040"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            792..>1944
                     /gene="mnmA"
                     /locus_tag="P5293_09045"
     CDS             792..>1944
                     /gene="mnmA"
                     /locus_tag="P5293_09045"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRR50078.1"
                     /translation="MKVAVGLSGGVDSSVAALLLKRAGHDVIAVFMQNWHDTTGTLHG
                     DCSWEEEKIVAEMVAKKLDIPFHFADLSDIYRERVVDYMFREYSAGRTPNPDVMCNRE
                     IKFDAFMNVAFDLGADYVATGHYSRREVIKNENGTEISRLLAGKDSNKDQSYFLCQLS
                     QNQLSRVIFPIGGMLKPEVRHLAALTGLPSAEKKDSQGICFVGKVDLPLFLQQKLAAK
                     EGDVIEISADFYAGTSYSAMLEEGYGLLDRFSLDELTSEYKYNHADGKIIGRHQGAHF
                     YTIGQRKGLNIGGHAEPLFIIGTDVTRNLIFVGEGNTHPGLFRKGLFISEENIHWIRE
                     DLRMTEGEVRDYQVRIRYRQPLQNAELFKTNSGLYIIFDLPQRSVTPGQF"
BASE COUNT          566 a          308 c          464 g          606 t
ORIGIN      
        1 tcgatttttt gcgaaaagag agggttgtaa aatatcatca ttaatgatgc ggcgaagagg
       61 tatttcttct gaaataacat atgtgaataa tgctcaattc tgcaaaatta ctaattttgt
      121 atcaattact ataaatacat atgaataata aaagtattac tatcccttat attgagtgtg
      181 gtttcggtga agaattggat gaaaaggaca atgaattgtt aaatgcagct aaagaggcca
      241 cgaaactttc atatgctccc tattcgggat tttgtgttgg tgccgcattg agactggcaa
      301 atggtgaaat tatttctgca agtaatcagg agaatgctgc atatccatcc ggactttgtg
      361 cagagcgtgt agctatattt tatgctaatt caaaatatcc tgatgtggct gttgaatctt
      421 tggcagttac tgctgctgtg gacgggaaga tatgtgagat gcctacatat ccttgtggag
      481 catgtcgtca ggttattgca gagtcagaaa agagattcgg taaaagaatc agaatcattg
      541 caggaggaga gaagattatt cagataatag atggaatcaa tggtttgttg ccttttgctt
      601 ttgacaattt gccaagatag tttttcgtca ctttgaaatt tattgtatct ttgtgcgggg
      661 taagtcttat acgaccagct cccactgaac tcccccaggg ccggaaggca gcaagggtag
      721 gtggttgtag cggtgcgata tattcagctt gccccttttt tttattttaa tagtctattg
      781 acatagctac tatgaaggtg gcagttggcc tttcaggcgg agttgattcg agcgtggctg
      841 ctcttttgct taaaagagcg ggtcatgatg tgattgctgt atttatgcaa aattggcatg
      901 atacaacagg tactcttcat ggagattgct cttgggaaga ggagaagatt gtagctgaaa
      961 tggttgccaa aaaacttgat attccttttc attttgcaga tttgagcgat atttacaggg
     1021 aacgtgtagt ggattacatg tttagagaat atagtgctgg cagaacaccc aatccggatg
     1081 ttatgtgtaa cagagagatt aaatttgatg cttttatgaa tgtcgctttt gaccttggag
     1141 ctgattatgt tgcaacagga cattattctc gcagggaggt gattaaaaat gagaatggaa
     1201 ctgaaatttc acgtctcctt gccggtaagg acagtaacaa ggatcaaagc tattttcttt
     1261 gccagctttc tcaaaatcaa ctgtctagag tgatttttcc cataggaggt atgctgaaac
     1321 cggaggtaag acatcttgct gctttaacag gacttccttc ggctgaaaag aaggattctc
     1381 agggaatatg ctttgttggt aaagtcgatt tgccgctgtt tcttcagcaa aaacttgctg
     1441 caaaggaggg ggatgtaatc gagatttctg ctgactttta tgctggcaca tcatattctg
     1501 caatgcttga agaaggttat ggattattag acagattttc acttgatgag ctcacatcgg
     1561 agtataagta taaccatgcg gatggaaaaa ttattggcag acaccaaggt gcacattttt
     1621 acacaatcgg gcaaagaaaa gggcttaata ttggagggca tgctgaacct cttttcataa
     1681 ttggaacaga tgtcaccaga aatttgattt ttgttggaga aggcaatact catcctggtc
     1741 tttttcgtaa gggacttttt atttcagagg aaaatattca ctggattaga gaggatttga
     1801 gaatgacaga gggagaggtt cgtgactacc aagtgagaat cagatacagg cagccacttc
     1861 agaatgcaga acttttcaaa acaaatagtg ggctctatat tatttttgat ctgccacaaa
     1921 ggagtgtgac tcccggtcaa tttg
//
LOCUS       DAOTQL010000235         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_47832_length_1917_cov_4.740602, whole genome shotgun sequence.
ACCESSION   DAOTQL010000235 DAOTQL010000000
VERSION     DAOTQL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47832_length_1917_cov_4.740602"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="P5293_09050"
     CDS             complement(<1..915)
                     /locus_tag="P5293_09050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRR50079.1"
                     /translation="MKILDFPDKFVEYLIEAAGDETACRVLETISESSPETAVRINDL
                     KVDDPFSLGLKIESKIPWCNNGYYLKERADFTFDPLFHAGGYYVQEPSSMFLAVLEPI
                     FREVKPKSFLDLCAAPGGKSTHLVSMVARSIVGSENLCSFNSMCSVVCNEVIGSRASV
                     LAENIAKWGVPGVTVTSHDPRYFSKGGWLFDFILVDAPCSGEGMFRKDTKVISEWSES
                     GVNLCAARQRRILSDIWGALAPGGLLAYSTCTFNRRENDDNVRWLKSEFGALSYDLGL
                     AEDVIESWGILVTQEGGYQFFPGLARGEG"
     gene            complement(916..>1917)
                     /locus_tag="P5293_09055"
     CDS             complement(916..>1917)
                     /locus_tag="P5293_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRR50080.1"
                     /translation="EIGSWRAGTIIGLFPGTGVDSTRYGGYYTIEQMKDIVKYAADRY
                     ITIVPEIDIPGHSMAIISAYPELSTTPDIPKQPAITWGIFNRQNNVLLPSEQTFKFLK
                     DVFNELMDIFPGEYIHVGGDECSPIWWRDSRETQAFMKNYGIKDETELQGYFLKRVYD
                     VVKARGRKLVGWDEIMESPSYLEKDMVVMSWRDARHGWKAAEMGNKTIIASVNQHYFN
                     TAQRKDETALCHKNFFVPLDSVYYFEPIPDIYNIAYEENILGGEGCMWTEYFPDKAAV
                     EYAIFPRMSAFSEVYWSQSKNKNWPKFMLKLVEQFNRYDLWGAGYCTYIFESGHYSR"
BASE COUNT          613 a          499 c          296 g          509 t
ORIGIN      
        1 tccctctccc cttgcaagac ccgggaaaaa ttgatatccc ccttcttgag tcactaatat
       61 tccccaactc tcaattacat cttcagccaa ccccaaatca taggagagtg ccccaaattc
      121 actctttaac catctcacat tgtcatcatt ctccctgcgg ttgaaagtgc atgtagaata
      181 agccagaaga cctcccggag ccaatgcacc ccaaatatcc gacaagatcc ttctctgtct
      241 tgcagcacat aaatttacgc cagattctga ccattccgat atcactttag tgtccttacg
      301 aaacataccc tcacccgaac atggagcatc taccagaata aaatcgaaca accatccacc
      361 ttttgaaaaa tatctcgggt catgacttgt cacagttact ccgggcacgc cccatttagc
      421 aatattctct gcaagaacgg aagccctgct gccaataacc tcattgcaaa caacagaaca
      481 cattgaattg aatgaacaca aattctcaga accgacaata ctccttgcca ccatagagac
      541 taaatgagtg cttttaccac caggagcagc acaaagatcc agaaaagact tgggcttcac
      601 ctctctgaaa atgggctcca aaacagcaag aaacatagat gaaggctcct ggacatagta
      661 tcctcccgca tgaaatagag gatcaaaagt aaaatcagcc ctttccttca aataataacc
      721 attattgcac caaggaatct tactttcaat ttttaaccca agagaaaaag gatcatcaac
      781 cttcaaatca tttattctca cagcagtttc aggactgctt tcagagatag tctccagaac
      841 ccggcaagct gtctcatcac cggcagcctc aatcagatat tccacaaact tgtcaggaaa
      901 gtccaaaatt ttcacttacc ttgaatagtg accagattca aaaatatatg tacaataccc
      961 tgctccccac aaatcataac ggttaaactg ttccacaagc ttcagcataa atttcggcca
     1021 gtttttattt ttgctttgag accagtaaac ttcagaaaaa gcagacattc ttggaaatat
     1081 tgcatattcc acggctgctt tgtcaggaaa atattcagtc cacatacaac cctcacctcc
     1141 aagaatattc tcttcgtaag caatattgta tatatcagga ataggctcga aataatatac
     1201 agaatcaagt ggtacaaaaa aattcttgtg acaaagagct gtctcatcct ttctctgtgc
     1261 agtattaaaa tagtgctggt ttacagaagc aattattgtc ttattaccca tctctgcagc
     1321 tttccagcca tgcctggcat ctctccagct catcacaacc atgtcttttt caagatagct
     1381 cgggctctcc ataatttcat cccaaccaac aagcttcctc ccacgagctt taacaacatc
     1441 atagactctc ttcaaaaaat agccctgaag ttcagtttcg tctttaatcc cataattctt
     1501 cataaaagcc tgagtctctc tagaatctct ccaccagata ggagaacatt cgtcaccacc
     1561 aacatgtata tattccccag gaaatatatc catcagttca ttaaatacat ccttcaaaaa
     1621 cttgaaagtc tgctccgaag gaagcaaaac attattctga cgattgaaaa ttccccatgt
     1681 aatagcgggt tgcttgggaa tatccggagt agtacttaat tcaggatagg ctgaaataat
     1741 tgccatagag tggcccggaa tatcaatttc gggaacgatg gtaatataac gatcagcagc
     1801 atattttaca atatccttca tctgctcgat agtgtaatag ccaccgtatc tggtactgtc
     1861 cacaccagtc ccagggaaaa gcccgatgat tgttccggct ctccatgaac caatctc
//
LOCUS       DAOTQL010000236         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_48304_length_1904_cov_4.187128, whole genome shotgun sequence.
ACCESSION   DAOTQL010000236 DAOTQL010000000
VERSION     DAOTQL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48304_length_1904_cov_4.187128"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="P5293_09060"
     CDS             complement(<1..1300)
                     /locus_tag="P5293_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRR50081.1"
                     /translation="MKKISKICCIGAGYVGGPTMAVIAFKCPEINVTVVDVNPERIAK
                     WNSDDLSQLPVYEPGLQDVVAKTRGKNLFFSTDIESGIKNAEMIFISVNTPTKTYGKG
                     KGMAADLKYVELCARQIADVADEEKIIVEKSTLPVRTAQTIRTIMENCGKNIQFQILS
                     NPEFLAEGTAISDLENADRVLIGGDHTSEEGREAIDSLAAIYEHWIPSEKIIKTNLWS
                     SELSKLTANAFLAQRVSSINSMSALCELTEADVDEVAAAIGADKRIGPKFLKASVGFG
                     GSCFQKDILNLVYICRSLGLDEVADYWQGVIDINNYQKRRFAEKIVTNLFNTVAGKKI
                     AFLGWAFKKDTNDTRETPAMYVADYLLEETAKITVYDPKVNHLQMLSDVNHLGTRDEA
                     INEKLLTCSSDPYETCHDAHAVAILTEWDEFKGYNWQRIFD"
     gene            complement(1474..>1904)
                     /locus_tag="P5293_09065"
     CDS             complement(1474..>1904)
                     /locus_tag="P5293_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRR50082.1"
                     /translation="FKMSLIEIAWGLGMLVGGIFISISKSKNKESILINSSYIVLGTT
                     FLLSGFLPSNAFYVFAALSAIGGIAGAVFWGAFTVLLQRSIEPGVLGRVFSIHGSLIM
                     IPAMFSLVATGYIADAIGITNTFICGGGALILIGAFSFRL"
BASE COUNT          599 a          402 c          312 g          591 t
ORIGIN      
        1 tgtcaaaaat cctttgccag ttataacctt tgaattcgtc ccattcagtt aaaattgcca
       61 ctgcatgggc atcatgacat gtctcgtaag gatctgaaga gcaggttaat aatttttcat
      121 taatcgcttc atctctggtt cccaaatgat tgacatcgga aagcatttgc aaatgattga
      181 ccttagggtc ataaacagtt attttggcag tctcttctag cagataatca gccacataca
      241 tggcaggtgt ttcacgagta tcatttgtat cttttttgaa agcccaccct aaaaatgcaa
      301 ttttctttcc tgcaacagtg ttaaaaaggt ttgtgacaat tttttcagca aatcttcttt
      361 tttgataatt attaatgtca ataacgcctt gccaataatc agcaacctcg tctaaaccaa
      421 gtgatctaca gatatataca agattaagaa tgtctttttg aaaacaagaa ccaccgaagc
      481 ccacagatgc tttgagaaat tttggtccaa tcctcttatc tgcgccaata gctgcagcta
      541 cctcatctac atcagcttca gtaagttcac ataaagcact catagagtta attgaagaaa
      601 ctctctgtgc aagaaaagca ttagctgtta atttggaaag ctctgatgac cagaggtttg
      661 ttttaattat tttctctgaa ggtatccaat gctcatatat agctgccaga gaatcaatgg
      721 cttctctacc ttcttcagaa gtatgatctc cgccaattaa cactctgtcg gcattttcaa
      781 gatcagaaat tgctgttcct tctgccaaaa attctggatt tgataaaatc tggaattgga
      841 tatttttgcc acaattctcc ataattgttc gaattgtctg agccgtacga acaggcagag
      901 tcgatttttc aacaataatt ttttcctcgt cggccacatc agcaatttgc ctggcacaaa
      961 gttcaacata tttaaggtca gcggccatcc ccttgccttt gccataagtc tttgtaggag
     1021 tgttaactga gataaaaatc atttcggcat ttttgatacc actctcgata tctgtcgaaa
     1081 aaaacaaatt tttgcctctt gttttagcca ctacatcttg aagaccaggc tcatatacag
     1141 gcaattggct caagtcgtcg ctattccatt ttgcaatcct ttcggggtta acgtcaacca
     1201 ctgtgacatt tatctcaggg catttgaaag caataacggc catagttgga cctccaacat
     1261 aaccggctcc aatgcagcaa atttttgata tttttttcat tatttagatt tggaaaaaat
     1321 tatgacaaat atacataaat gtggaaacaa ccggctctga cctgtcggag tttgtcgtaa
     1381 aacgcacata ttcaaatgtt taaattttaa agtttattga agtgattttt ttaaaacaca
     1441 ctaaatctgc gttttacgac aaactccgac aggtcagagc ctaaacgaga atgctccgat
     1501 aagtattaat gcgcctcctc cacaaataaa ggtatttgta atacctattg catcagcaat
     1561 ataacctgtc gccacaaggc taaacattgc tggaatcatt attaaactac catgaataga
     1621 aaacaccctg cctaatactc ctggctcaat gctcctttga agaagcactg taaatgcacc
     1681 ccaaaaaacg gctccggcaa ttccaccaat tgcagacagt gctgcaaaga cataaaatgc
     1741 attcgaaggt aaaaaacctg ataataaaaa agttgtgcct aaaactatgt acgatgaatt
     1801 aataagtatg gattccttat tctttgattt tgaaatactt atgaatatcc ctccaacaag
     1861 cattccgagt ccccaagcta tttcaattaa actcatttta aagg
//
LOCUS       DAOTQL010000237         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_49674_length_1868_cov_4.361831, whole genome shotgun sequence.
ACCESSION   DAOTQL010000237 DAOTQL010000000
VERSION     DAOTQL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49674_length_1868_cov_4.361831"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="P5293_09070"
     CDS             complement(<1..502)
                     /locus_tag="P5293_09070"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HRR50083.1"
                     /translation="MLLDLLLPRICPVCGRVLLKGEKQLCLKCLSEIPFTYFWTWSGN
                     PAENRFLGKVDIVRAASLFYYRDGSCYKNIIHLFKYKGRRKLGKFMGEMLGEKLLSNG
                     IYEDIDVIIPVPLHPLKRWKRGYNQAEIIALAIGNVIRKPVDCSSLVRRRYTVSQTRK
                     SASQREK"
     gene            complement(502..>1868)
                     /locus_tag="P5293_09075"
     CDS             complement(502..>1868)
                     /locus_tag="P5293_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRR50084.1"
                     /translation="TYDYRFLWRHRSINRKIDNGILIGEVYENSPAAKSGLVAGDIIL
                     KIDNQDISGLIVDSCSARMRGRPGSQVAFLVKKLRGGDTVEVKVIRDKVHFPDVAYSG
                     FVNDTTGYIRLNGFTFGGSKDFRRELLELKKNSDMKRLMIDIRGNGGGLLDEAVNIVS
                     LFVPKGTKVVSAIGRTKQSSMDYYTKDEPVDTSLAIIVLVNSGSASSSEIVAGALQDL
                     DRATIVGTRTYGKGLVQSIRDVGYGDRIKLTTAKYYTPSGRCVQAIDYSHRNEDGSVG
                     NIPDSLRRPFTTLKGRTVYDGGGITPDVIINPQTVPRAVMSLIFAEVLSDYSILYFRD
                     HISIAKPKDFKLTDAEYEEFVSYASSRNFDGRSASEVELDRLVSILKEESMYDEFKDK
                     VTALEENVKLTKEEMLRRNSNIIRPFLEEEIVNRYYYERGQIESVLRNDYQVKKALEV
                     KLIN"
BASE COUNT          555 a          433 c          288 g          592 t
ORIGIN      
        1 ttttttctct ttgagatgca ctttttctgg tttggctcac agtatagcgc cttctgacaa
       61 gtgaagagca atcaacaggt tttcttataa catttccaat ggcaagggca attatctctg
      121 cctggttata ccctctcttc cagcgtttaa ggggatgtaa aggaacagga attataacat
      181 cgatgtcttc gtaaatacca ttgctcaaaa gtttttctcc aagcatttcc cccataaatt
      241 tgccgagttt gcgacggcct ttgtacttaa aaaggtgaat tatgttttta taacagcttc
      301 cgtctctata gtaaaacaaa gatgctgccc tgacaatatc gactttgcca agaaacctgt
      361 tttcagcagg attgcctgac catgtccaaa aataagtgaa aggaatctca gaaagacatt
      421 taagacaaag ttgtttctcc ccttttaaaa gtactcttcc acaaacaggg catatccttg
      481 gcagaagcaa atcgagcaac atcagttaat caatttgact tcaagggctt tcttaacttg
      541 ataatcgttt cttaatacac tctctatctg acctctttca taataatatc tattaacaat
      601 ctcctcctct agaaaaggcc ttataatatt tgagtttctt cgcaacatct cctccttggt
      661 aagtttaaca ttctcttcaa gggctgtaac tttgtcttta aattcgtcgt acatcgactc
      721 ttcctttaaa atagatacta acctgtccaa ctcaacttct gaagcactcc taccatcgaa
      781 attacgtgaa gaagcataag aaacaaattc ttcatattcg gcatcagtaa gtttgaaatc
      841 ttttggtttg gcaatcgaga tatgatctct aaagtataga attgaataat ctgaaagcac
      901 ttcggcaaaa ataagtgaca taactgctct cggaacggtt tgaggattaa taataacatc
      961 aggagtgatt cctcccccat cataaactgt cctgccttta agtgttgtaa acggtctacg
     1021 taatgaatca ggaatatttc caacacttcc atcctcgttt ctatgtgaat agtctattgc
     1081 ctgaacacac cttccacttg gtgtgtaata tttagctgta gtaagcttta ttctgtcgcc
     1141 gtaaccaaca tctcttattg actgcaccaa gcctttacca taagttcttg ttcctactat
     1201 cgttgctctg tcaagatcct gaagagctcc tgcaacaatt tctgatgagg atgcagaacc
     1261 ggaattaaca agaactatta tagccagaga tgtgtcaaca ggttcatcct tagtatagta
     1321 atccatgctc gattgctttg ttcttcctat ggcagaaaca actttagtcc ccttaggcac
     1381 aaacaatgag acaatattaa ccgcttcatc cagcaatccg cctccattac ctctgatatc
     1441 aatcatcaat cttttcatat cggaattttt cttgagttca agcaattccc gacggaaatc
     1501 tttcgaacct ccaaaagtaa atccattaag tcttatgtat cctgtagtgt cattcacaaa
     1561 tccgctataa gcaacatccg gaaaatgaac cttatccctt ataactttta cctccactgt
     1621 gtcacctcct ctaagttttt tgacaagaaa agcaacctgc gaacccggtc ttcccctcat
     1681 ccttgcacta caagaatcta caataagacc cgatatgtcc tgattatcaa tcttaagaat
     1741 gatatctcca gctacaagcc ctgacttagc tgcaggcgaa ttttcataaa cttctcctat
     1801 caaaatgcca ttgtcaattt ttctattaat gctcctatgc cgccataaga acctgtagtc
     1861 ataagttc
//
LOCUS       DAOTQL010000238         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_49752_length_1866_cov_3.782441, whole genome shotgun sequence.
ACCESSION   DAOTQL010000238 DAOTQL010000000
VERSION     DAOTQL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49752_length_1866_cov_3.782441"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="P5293_09080"
     CDS             complement(<1..641)
                     /locus_tag="P5293_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRR50085.1"
                     /translation="MEKSNNSPFEEWGLGKLERPLVIAGPCSAESEEQVVETATQLKT
                     LGIEVFRAGIWKPRTRPNQFEGVGISGLEWMKRVQKEVGMKVSTEVGNVKHVYDSLRH
                     GVDIIWIGARTSADPFAMQEIADALRGVDIPVLVKNPVNPDLNLWIGAIERISAAGIR
                     RVGAIHRGFSTYDTTRYRNIPQWQVPIELKQKMPEIPMICDPSHMGGKRELIQ"
     gene            complement(643..1497)
                     /locus_tag="P5293_09085"
     CDS             complement(643..1497)
                     /locus_tag="P5293_09085"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRR50086.1"
                     /translation="MTVTIIGIGLIGGSAAIDLRKRGFAATILGVDNDKINASAALSL
                     GLVDEICTLEEGVKRGDLVIISTPANATLTLLPKILDIAGNKTIITDVSSTKFEICQK
                     VKNHVNRGRFVASHPMAGTEFSGPWAAISGLFDGRGAIVCDPEQSDKDALDTIQKMYE
                     TLNMRIIYMTSSSHDTHAAYVSHISHISSFALALTVLEKEQDEKNIFDMASGGFDSTV
                     RLAKSSSDMWVPVFLQNKENVLTVLRTYIDKLREFETFIESEEEEKIKSLIESSNKIR
                     KVLNCNKN"
     gene            complement(1511..>1866)
                     /locus_tag="P5293_09090"
     CDS             complement(1511..>1866)
                     /locus_tag="P5293_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR50087.1"
                     /translation="SGMYKPLQMAAAQALSCNEDWFNRINDEYKRRRELVWKIFEATG
                     STYDKRQSGLFVWAKIPQDYENSTSFSNKYLYESNVFIAPGFIFGTNGEGFARISLCC
                     NIETLNEALNRIKKK"
BASE COUNT          469 a          411 c          356 g          630 t
ORIGIN      
        1 tcctgaatta actctctttt gccgcccata tgacttggat cacaaatcat aggtatctca
       61 ggcattttct gctttagttc gataggtact tgccattgag gaatattcct gtatcgtgtt
      121 gtgtcgtaag ttgaaaaacc tctatgtatt gctccaactc tgcgaatccc tgctgctgaa
      181 attctctcta ttgctccaat ccataagtta agatctggat taacagggtt tttgacaagg
      241 actggaatgt ccactcccct tagggcatct gcaatctctt gcatggcaaa tggatctgca
      301 gacgttctgg ctccaatcca gattatatca actccgtgtc tcaatgagtc gtatacgtgt
      361 ttaacatttc caacttcagt agatactttc attccaacct ctttttggac tcttttcatc
      421 cattcgagac cggaaattcc tactccttca aattgattcg gtctggtacg tggcttccat
      481 attcctgcac ggaaaacctc aatacccaac gttttgagtt gtgtggcagt ttctacaacc
      541 tgttcctcag attctgcact gcagggaccg gcaatgacta ggggacgttc taacttcccg
      601 agcccccatt cttcaaaagg tgaattattg ctcttttcca tatcagtttt tattacaatt
      661 caatactttt cttattttat ttgaactctc tataagagat ttgattttct cttcttcttc
      721 tgattcaata aaggtctcga actctctcag tttatcaata tatgttctga gaacagtaag
      781 tacgttttct ttgttttgca ggaagacagg cacccacatg tcggaagagc ttttagcaag
      841 tctgacagtc gaatcgaaac caccgctggc catgtcaaaa atattttttt catcctgttc
      901 cttttcaagt actgtcagtg caagagcaaa ggaacttatg tgagatatat gagagacata
      961 ggcagcatgc gtgtcgtgac tggagcttgt catgtagatg attctcatat taagggtttc
     1021 atacattttt tggattgtat caagggcatc tttgtcagat tgctctggat cacatacaat
     1081 agcacctctc ccgtcaaaaa gtccggaaat tgcagcccat gggcctgaaa actcagtccc
     1141 tgccattgga tgagaggcaa caaaacgtcc tcggttaaca tgatttttaa ctttttgaca
     1201 tatctcaaat ttagttgagc tcacgtctgt aataatcgtt ttgtttccgg ctatgtcaag
     1261 aatctttggg agcagagtga gagttgcatt ggcaggggtg gaaattataa cgagatcgcc
     1321 tcttttgaca ccctcttcga gggtacatat ttcgtctacc agtcctagtg ataatgctgc
     1381 gctggcgttg atcttgtcat tgtcaactcc caatatggtt gcagcaaaac ctctttttct
     1441 gagatctatt gcagcggaac cacctattag accaatgcct ataattgtta ctgtcatctt
     1501 agatcaattt ttattttttc ttgattctgt tgagagcctc gttaagagtc tctatattgc
     1561 agcataggga tattctggca aatccttccc catttgtccc gaaaataaaa ccaggagcaa
     1621 tgaatacgtt actttcatac agatatttat tggaaaagct ggtggagttt tcgtaatcct
     1681 gtggaatttt tgcccagaca aacagtccac tctgtcgctt gtcataggtt gatccggttg
     1741 cttcaaatat tttccatacg agttctctcc ttcttttgta ctcgtcgttt attctattga
     1801 accaatcctc gttgcatgaa agtgcttgtg cagcagccat ttgcaaaggt ttgtacattc
     1861 cactgt
//
LOCUS       DAOTQL010000239         1850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_50399_length_1850_cov_3.767131, whole genome shotgun sequence.
ACCESSION   DAOTQL010000239 DAOTQL010000000
VERSION     DAOTQL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50399_length_1850_cov_3.767131"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="P5293_09095"
     CDS             <1..763
                     /locus_tag="P5293_09095"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HRR50088.1"
                     /translation="MQTYLDADYDIRVHVLGDKVIAAMKRFKIKKDFRSNYSLGGKIE
                     KVDLTEEQEKIAINAAQAVGAVWAGVDIITNKKDKKNYVIEINSSPGTEGIEKATRKD
                     IVSKVINFVLNKNNWKRPATECGYLEVVKVEEIGEMDAKLDTGNGSYCVIHADEYEVD
                     GDKVNWRIGKREFSHKIEGIKTINVGGIETKKEDRPVLSLKVTFNNETYDMKFTLSNR
                     KGFSTPILLNRKFLRLANLSVNSAKKYVLTTKKSV"
     gene            complement(755..1363)
                     /locus_tag="P5293_09100"
     CDS             complement(755..1363)
                     /locus_tag="P5293_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRR50089.1"
                     /translation="MTRKFKKEAQEVRFIIDPQEIIKDIQPQRSGKRLDRIIYIVGNL
                     DENLAREVNERLLDYQAEAPLKEITLILDCYGGEVDSMFAVTDMMNIIMSPIRTICIG
                     KAMSAAAFIFINGKKGRRFMTPHSRLMFHQISSWHGGTLADVVIDTEEIKFLQNQMVE
                     EIAMRSKLSSKEVIKLIDRNTYLRPEEAIKLGMCDGLIKRLN"
     gene            complement(1347..>1850)
                     /locus_tag="P5293_09105"
     CDS             complement(1347..>1850)
                     /locus_tag="P5293_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50090.1"
                     /translation="KNVMEMILRGETDDNILMTIEQYKKELKQVYPEEISVNIGASDI
                     DKYVNKDGEIEKGTPFHLRGINNYRKLLKHLKLEKKYEDIYSGAKAKVVYLKKNPLNL
                     DILSFLRWPKEFDKVVQIDYDKMLEKYFLSKIEILLEPMNKKELLSGSSGAGLNLFFG
                     EENDKKI"
BASE COUNT          614 a          356 c          295 g          585 t
ORIGIN      
        1 tatgcaaaca tatcttgacg ccgactatga cataagagtt catgttctcg gtgacaaggt
       61 aattgctgct atgaaaaggt tcaagataaa gaaggacttc agatcaaatt actcacttgg
      121 tggaaaaata gagaaagtgg atctcacaga ggaacaggaa aagattgcca taaatgctgc
      181 tcaagccgtg ggagctgtct gggcgggtgt tgatataatc acaaacaaga aagacaaaaa
      241 gaattatgta atagaaataa actcttcgcc aggaacggaa ggtatagaaa aggccacgag
      301 aaaagatatt gtaagtaaag ttattaattt tgtgctgaat aaaaataact ggaaaagacc
      361 ggccacagaa tgtggttact tagaagtggt caaggtggaa gaaattggtg agatggatgc
      421 aaaattagat acgggcaatg gttcatattg cgtcatacac gccgatgaat atgaagttga
      481 cggagacaaa gtaaattggc gcattggtaa aagagaattt tcccataaaa tagaaggcat
      541 caaaacaata aatgtaggtg gcatagaaac caaaaaagag gacaggcctg ttctatcctt
      601 gaaagtcact ttcaacaatg aaacatacga tatgaaattt actttaagta atagaaaagg
      661 attttctaca cctatattac tcaacagaaa atttttgcgt cttgcaaacc tatcagtaaa
      721 ctcagcaaaa aaatatgtac tgacaacaaa gaaatcagtt tagcctttta ataagaccgt
      781 cacacattcc aagttttatt gcttcttccg gtcggaggta agtattccta tcaatcaatt
      841 ttataacttc ctttgacgaa agtttacttc gcatcgcaat ctcttccacc atctggttct
      901 gaagaaattt tatctcttct gtatcaataa ccacatctgc aagtgttcca ccatgccatg
      961 aacttatctg atgaaacatc aatctggaat gtggtgtcat aaatcgccta ccttttttac
     1021 cgttgataaa aataaaagcg gcagctgaca tagcctttcc aatacagatt gtccgaattg
     1081 gtgacatgat tatattcatc atatcagtaa cagcaaacat actgtcaacc tcaccaccat
     1141 agcaatctaa tatcaatgta atctctttta acggtgcttc agcctgataa tccaatagcc
     1201 tttcatttac ttctcttgca agattttcat caagattacc cacaatgtat ataatcctgt
     1261 ccaatctttt gccagatcgt tgaggttgta tatcttttat tatttcctgt ggatcaatta
     1321 taaatcgtac ttcctgtgct tcttttttaa attttcttgt cattttcttc tccaaagaat
     1381 aaattcaatc ccgcaccaga tgaaccagag agtaattcct ttttattcat tggttccaga
     1441 agaatttcaa tttttgatag aaagtatttt tccaacatct tatcataatc tatctgaaca
     1501 accttatcaa attcttttgg ccatctcaag aatgataata tatccaaatt taatggattt
     1561 ttcttgagat agacaacctt cgctttagct ccactataaa tatcctcata tttcttttca
     1621 agtttcaaat gttttaggag tttcctataa ttatttatac ccctcaaatg gaagggagtt
     1681 cccttctcga tttctccatc cttgttcaca tacttatcaa tatcactcgc cccaatatta
     1741 actgaaatct cctcaggata aacctgcttc aattcttttt tgtattgttc tattgtcata
     1801 agaatgttat catccgtttc ccccctcaaa atcatttcca taacattctt
//
LOCUS       DAOTQL010000240         1845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_50625_length_1845_cov_2.831285, whole genome shotgun sequence.
ACCESSION   DAOTQL010000240 DAOTQL010000000
VERSION     DAOTQL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50625_length_1845_cov_2.831285"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /locus_tag="P5293_09110"
     CDS             complement(<1..1784)
                     /locus_tag="P5293_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRR50091.1"
                     /translation="MGAFVIPFFFLGIALYCLKIKRISLLKLFFVTVFGAIIFSVFFS
                     YVFSFTRFNNLFGSGVGGSYGYYVNKWLMDMLGASGAGALVVVLMIGWFSLVSYGVVR
                     WFNRVIAKTFHPEKDEIAVAETEVDAPDNEHIPLMGVAAAAGNEAAGQSDDILFEIIE
                     NEDGTDTDIDADTDEDFIKNLPQGSLETLFDPRLDLSNYKSPPLSLLEEYKDKVYEVS
                     REELELNNKKIVKTLSDYKIGIERIFARPGPTVTLYEIVPQAGVRISQIKRLEDDIAL
                     QLAAKGVRIIAPIPGTNYVGIEVANNKPSIVSMRSVLEDPKFKVAAANFDLPVALGRT
                     ISNEVLTIDLAKMPHLLVAGATGQGKSVGLNAIITSLLYTKHPSELKFVLVDPKKVEL
                     SLYEKLEHHFLAKLPEGEEAIITDTQKVIYTLKSLLIEMESRYDLLTLARVRSIKEYN
                     DKFLSRKLNPLKGHRFLPFIVVIIDEFADMLMTAGREVEDPITRLAQKARAIGIHLVI
                     ATQRPSVNIITGLIKSNFPARMAFKVISSIDSRTILDTTGANQLVGRGDMLFLYNVEI
                     TRVQCAFIDTREIDRIVDFISSQQGYATA"
BASE COUNT          632 a          448 c          312 g          453 t
ORIGIN      
        1 gcagtagcat aaccctgctg actgctgata aaatccacaa tcctgtcaat ctccctggta
       61 tcgataaagg cacactgaac ccgcgtaatc tccacattat aaaggaaaag catatcacct
      121 cgccctacga gttgattagc tccagtagta tcaagaatag ttctcgaatc aatacttgaa
      181 ataaccttga aagccatcct tgcagggaaa ttggacttaa taagaccggt aataatattt
      241 acagaaggac gctgagtagc tatcaccaaa tgaataccta tagcacgagc cttctgagca
      301 agccgtgtaa taggatcctc aacctcgcgg ccggcagtca taagcatatc ggcaaattca
      361 tcaattatca caacgataaa cggaagaaaa cggtgaccct taagaggatt aagtttacga
      421 gacaaaaatt tgtcattgta ctccttaata gaacgaactc ttgcaagagt aagaagatca
      481 taccgactct ccatctcaat caacagagat ttaagagtat agataacctt ctgagtatct
      541 gtaataattg cctcctctcc ttcaggaagt ttcgccaaaa aatgatgctc aagcttttca
      601 tatagagaga gctccacttt ttttggatca accaggacaa atttcagctc agaaggatgc
      661 ttggtgtaaa gaagagaagt aataatagca ttaagcccca cagacttgcc ctgtcctgtt
      721 gcaccagcaa ccagcaagtg aggcattttt gcaagatcga tagtaagaac ctcattggaa
      781 atagttctcc caagagctac aggcaaatca aaattggcag cagcaacctt aaacttggga
      841 tcctccagaa cagaccgcat agaaacaata ctcggcttgt tattcgctac ctcaatcccc
      901 acataattgg tcccgggaat aggagcaatg atacgcaccc cctttgccgc aagttgaaga
      961 gctatgtcat cttccagcct tttaatctga gaaatacgca ctcccgcctg aggcacaatt
     1021 tcgtataaag taacagtagg ccccggcctc gcaaaaattc tctcaatacc tatcttataa
     1081 tcactcagag tcttaacaat cttcttatta ttaagctcga gctcctcgcg agatacttca
     1141 taaactttat ccttatattc ctcaagtaaa gagagaggtg gagatttgta attagataaa
     1201 tccaaacgag gatcaaaaag agtctctaaa gagccctgag gaagattttt aataaaatcc
     1261 tcatctgtat cagcatcaat gtcagtgtca gttccatcct cattttcaat aatttcaaac
     1321 agaatatcgt cagattggcc ggcagcttca tttcctgctg ctgcagctac tcccatgagc
     1381 ggaatatgtt cgttatcagg cgcatccact tctgtttcag caactgcgat ttcatctttc
     1441 tcaggatgaa atgttttggc aattaccctg ttaaaccacc taacaacacc atagctcaca
     1501 agagaaaacc agccaatcat caacacaaca accaacgccc ctgctccgga agcaccgagc
     1561 atatccatca gccacttatt cacataataa ccgtatgaac cacctactcc actgccaaac
     1621 aaattgttaa atcttgtaaa actaaagacg taagaaaaaa atacagaaaa gataatagcg
     1681 ccaaaaacag tgacaaagaa aagcttcaac aagcttattc tcttgatttt aagacagtac
     1741 agagcaatcc ccaaaaagaa aaacggaatt acgaaagccc ccaatccaaa aagctccgac
     1801 aacaaaaaat tagcccagaa aaaacccaat tttccaccac cgttt
//
LOCUS       DAOTQL010000241         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_50657_length_1844_cov_5.217440, whole genome shotgun sequence.
ACCESSION   DAOTQL010000241 DAOTQL010000000
VERSION     DAOTQL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50657_length_1844_cov_5.217440"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /gene="proS"
                     /locus_tag="P5293_09115"
     CDS             <1..1301
                     /gene="proS"
                     /locus_tag="P5293_09115"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRR50092.1"
                     /translation="TGHQNAYFPLFIPKSFLSREAEHVEGFAKECAVVTHHRLMANPD
                     GPGVVVDPDAKLEEELVVRPTSETIIWNTYKNWIKSYRDLPILVNQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATSEEAIEETLKMVQVYAEFARKYMSMPVIVGRKTESERFA
                     GAIDTLCIEAMMQDGKALQAGTSHFLGQNFAKAFDVQFANKEGGLDYVWATSWGVSTR
                     LMGALVMAHSDNNGLVLPPKLAPIQVVMVPVIKSDGDFSNILENMSQIKKRLEGVGIS
                     VKIDDRDNLRSGFKFAEWELKGVPVRLTVGPRDLQNGTVEISRRDTLTKEIASQDGLE
                     TRISLLLEDIQENIYNKALKFRAENTFKVDDYNTFKEKIEDGGFLLCHWDGTAETEAK
                     IKEETKATIRCIPLDSEEEEGKCIYSGKPSHRRVVFARAY"
     gene            1352..>1844
                     /locus_tag="P5293_09120"
     CDS             1352..>1844
                     /locus_tag="P5293_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50093.1"
                     /translation="MATELPSRQQEILEALNKKAQLKLQVFNQTSEVFSQLKDIINEM
                     SNDLNDLLDNDNRRIRLEYRDRGKFEAEFKFADDVLIFSMHSDVFLFDRNHPIWKNEY
                     VKQDPTNSYCGIISIYNFLSDSLKYNRGEDLGYLVARVFVNKDKFFLVEGKRQRNKVV
                     SAFG"
BASE COUNT          609 a          272 c          429 g          534 t
ORIGIN      
        1 agacaggcca tcaaaatgcc tatttccctc tatttatacc aaaatctttt ttaagcaggg
       61 aggcagaaca tgttgaaggg tttgccaaag agtgtgccgt tgttactcat cacagactaa
      121 tggcaaatcc agatggtcca ggtgtggtag tagatcctga tgcaaaactt gaagaggagc
      181 ttgttgtaag gcctacatct gagacaatta tatggaatac atataaaaat tggattaaat
      241 catatagaga ccttcctatt cttgtcaatc aatgggcaaa tgttgtaaga tgggagatgc
      301 gtacaaggct gtttctcaga acaactgagt tcctttggca agagggtcat acagcacatg
      361 ctacaagcga agaggcaatt gaggagacat tgaaaatggt gcaagtttat gctgaatttg
      421 caagaaagta catgtcaatg cctgtaattg tgggaagaaa gactgaaagc gagagatttg
      481 caggagcaat agatactctt tgcatagagg ctatgatgca ggatggtaag gctcttcagg
      541 caggcacttc tcattttctc ggtcaaaatt ttgccaaggc ttttgatgtt cagtttgcca
      601 ataaagaggg tggacttgat tatgtatggg ctacttcatg gggagtttct acaaggctta
      661 tgggagcact tgttatggca cacagtgaca ataatgggct ggtgcttcct ccaaagcttg
      721 ctccgattca ggtagtaatg gttcctgtta ttaagagcga tggagatttt tccaatattt
      781 tggaaaacat gtctcaaatt aaaaaaagat tggaaggagt tggcatttca gttaaaattg
      841 atgataggga caatctgcgt tcaggcttca aattcgcaga atgggaactt aaaggtgttc
      901 ctgtaagatt gactgttgga ccaagagatt tacaaaatgg cactgttgaa atttcaagga
      961 gagatacatt aaccaaggag atagcaagtc aagatggtct tgaaacacga atttcacttt
     1021 tgttagagga tattcaggaa aatatttata ataaggcttt gaaattcagg gcagaaaata
     1081 catttaaggt agatgattac aataccttta aagaaaaaat tgaagatggg ggttttttgc
     1141 tgtgtcactg ggacggtact gcagagactg aagcaaaaat caaggaggag actaaagcaa
     1201 ctatcaggtg tatcccattg gattcagagg aggaggaggg caaatgcatt tatagtggta
     1261 aaccttcaca tagaagagtt gtttttgcaa gagcatacta aattgaaact tattttctat
     1321 ttttacagaa aaatgaatta taataactaa tatggcaacg gaattaccgt caagacagca
     1381 ggaaatactg gaagcgctta ataaaaaggc gcaacttaaa cttcaggtat ttaatcagac
     1441 ctcagaggtt ttttctcagt taaaggatat tattaatgag atgagtaatg accttaatga
     1501 tcttctcgat aacgacaaca ggaggataag gcttgagtac agggacaggg gtaaatttga
     1561 agcggagttt aaatttgctg atgatgttct aattttcagc atgcattctg atgtttttct
     1621 ttttgataga aaccatccca tatggaaaaa tgaatatgtt aagcaagatc cgactaattc
     1681 ttactgcgga atcataagta tttataattt tctttcagac tcccttaagt ataacagagg
     1741 agaggatttg gggtatcttg tggcaagagt ttttgtaaat aaagacaagt tctttcttgt
     1801 agagggcaaa agacaaagga acaaagttgt cagtgcattt ggaa
//
LOCUS       DAOTQL010000242         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_51012_length_1836_cov_3.258843, whole genome shotgun sequence.
ACCESSION   DAOTQL010000242 DAOTQL010000000
VERSION     DAOTQL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51012_length_1836_cov_3.258843"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="P5293_09125"
     CDS             complement(<1..1242)
                     /locus_tag="P5293_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRR50094.1"
                     /translation="MLIINNARKLALITIAIFTTSVIYGQAVKNIARPVGWKDNNTVL
                     LSGMRNMKRISYEYDTKTAQLREIEVKDDTAAGKDLEPYKLIKKGDKNPTISPDGKMI
                     AFTRNNDLYVIEIASGIEKRYTFDGSSLIKNGYASWVYYEEIFGRLSEYRAFWWSPDS
                     KKIAFYRFDDTNVPMFPIFNYSGQHGSVAETRYPKAGDPNPEVKIAIIDILSGKTLWA
                     DFDSKEDQYFGMPVWRPDSEALLIQWMNRDQNDFRLYSFSASSGSKTQIYRENQPTWI
                     EWIEEYKTGKQGLYFVRDFDLWEQIYYVDYNGGTLHKLTNGNFWGTKLLELNEDLCSI
                     YFTSRGETSVRNDVYFLKWKSNFQVESVKKTSVGDFNYTSVVFSPDKRQFAAIISNLK
                     TPPASVVVNSGMLKQIEISMGE"
     gene            complement(1245..>1836)
                     /locus_tag="P5293_09130"
     CDS             complement(1245..>1836)
                     /locus_tag="P5293_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HRR50095.1"
                     /translation="YNREIRYRILCREEHINKGDKVMVVKNCYQFAGNDVAELDFIAN
                     GDVAELVKISHFEERYGFHFADAVLKFSDYHDIEISAKVILDTLESESASLSSEQQKS
                     LFYGVFADYSEIRTKRKRLAAVREDNYFNAMQIKFAAAVTCHKSQGGQWKCVFIDNPF
                     WRDYLTLDDLKWLYTAITRGFEHVYLVNFNNKFFNN"
BASE COUNT          570 a          400 c          272 g          594 t
ORIGIN      
        1 ttcacccatg ctgatctcaa tctgctttaa catgccagaa ttgaccacaa ctgaggcagg
       61 cggagttttc aaattagata tgattgctgc aaactgtctc ttatcaggtg aaaatacaac
      121 tgaagtataa ttaaaatctc caaccgaagt cttttttaca ctttcaacct gaaaattact
      181 tttccactta aggaaatata catcgtttct cactgaagtt tcacctctcg acgtaaaata
      241 tattgagcac aaatcctcat taagctcaag taacttggta ccccaaaaat tgccatttgt
      301 cagtttatga agagtaccac cattataatc cacatagtag atttgttccc acaagtcaaa
      361 atctctgaca aaatataatc cctgtttccc tgttttatat tcctcaatcc attctatcca
      421 tgtcggctga ttttctctgt aaatctgtgt tttactgcca cttgaagcag agaaagaata
      481 gagacggaaa tcattctgat cacgattcat ccactgtatt aacaatgcct ccgaatcggg
      541 tctccaaaca ggcataccga aatactgatc ttctttactg tcaaaatccg cccataaggt
      601 ttttcctgac aaaatatcta ttatggcaat ctttacctcg ggatttggat cgcctgcttt
      661 gggatatcga gtttctgcaa cagaaccgtg ctgtcctgaa taattgaaaa tcggaaacat
      721 gggcacatta gtatcgtcaa atctgtaaaa tgcaatcttt ttgctgtcag gtgaccacca
      781 gaaagctctg tattcactga gacggccaaa aatttcttcg taatataccc atgatgcata
      841 tccgtttttt ataagcgacg atccatcaaa tgtgtagcgt ttttcaatac ctgaagctat
      901 ttcaatcaca taaagatcat tattgcgtgt aaaagctatc atcttaccat caggagagat
      961 agttgggttt ttatccccct ttttaatcaa tttataaggc tcaagatctt tgcctgcagc
     1021 agtatcatcc ttaacctcta tctcacgtaa ttgagcagtt ttggtatcat attcataact
     1081 tatccgtttc atgtttctca ttcctgagag caaaacagta ttgttatctt tccaacctac
     1141 aggtcttgca atgtttttaa ccgcctgtcc atagataact gaagtggtaa aaatggcaat
     1201 agttataagt gccaacttcc ttgcattatt aattataagc ataattaatt attaaaaaat
     1261 ttgttattaa aattcacaag ataaacatgt tcaaatcctc tcgtaatagc agtataaagc
     1321 catttaagat cgtcaagtgt caaataatcc ctccaaaaag gattgtcaat aaaaacacat
     1381 ttccattggc ctccttgtga tttatgacat gtgactgctg ctgcaaactt tatctgcatg
     1441 gcgttgaaat agttgtcttc cctcactgct gcaagtctct ttcttttagt ccgaatttct
     1501 gaataatcag caaaaacacc ataaaataga cttttttgct gctctgaaga caaagaggca
     1561 ctttcgcttt ccagtgtatc caatataact ttagcactaa tctctatatc atgatagtct
     1621 gagaatttca aaacggcatc tgcaaagtga aaaccatacc gttcttcaaa atgagatatc
     1681 ttaaccagct ctgcaacgtc accattagca ataaaatcaa gttctgcaac gtcattacca
     1741 gcgaactggt aacaattctt aacaaccatc accttgtcgc ctttattaat atgctcttcc
     1801 ctgcaaagaa ttctatatct tatctctcta ttgtac
//
LOCUS       DAOTQL010000243         1812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_51979_length_1812_cov_3.421742, whole genome shotgun sequence.
ACCESSION   DAOTQL010000243 DAOTQL010000000
VERSION     DAOTQL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51979_length_1812_cov_3.421742"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="P5293_09135"
     CDS             <1..693
                     /locus_tag="P5293_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRR50096.1"
                     /translation="GDARAKFRYVEGGSTLALYGLEWAGVDDETGKNVWYLNNDATPD
                     LTVNGRPATFSYSKASEVILGDVNPKLFGGINTDVKWKNFSAGLNFTYKIGGYTYDGA
                     GRDVTDDGYYWERIVSKDQYKNRWTPEHKDAKLPMRAAIDMEDVNQKSSRHMHKADYL
                     RLKNITIGYSLPKNLISKVNISDVRIYFNGSNLWTLSAYDVYDPEVNEYGSRGWETPI
                     SKTYTFGVEFTF"
     gene            711..>1812
                     /locus_tag="P5293_09140"
     CDS             711..>1812
                     /locus_tag="P5293_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRR50097.1"
                     /translation="MKKINIFFAILSIVLLSSCEGFLDVKPTNSADAGTSITTAADAK
                     VIINGLMSKMVSSNYYGRNFFLYGDAKGGDFAIRSQGRGNDALYVFNHSATSNSYSGY
                     WSQMYHCLLQINNLLANIEKIEAAGKGSATLSDYKGQALTARALIYFDLVRLYGKPYN
                     MDKNSLGVPLELTPLDASAQPTRATVTQVYDQILADLTAAAPLMSKTKTLGFLNYYAN
                     KAIQARVYLYMENYPAALTAAQEIINSGKYSLYSNANWVSSWSTENGSESIFELGVYL
                     SEADLGTSSLGYYLLRTGTLKGAMGWFMASDSFLARLNEDPSDVRWGIMDYDETYSET
                     GVKRYGSCRKYTGVDLKGDKGSPSAVNIKVIRL"
BASE COUNT          556 a          320 c          406 g          530 t
ORIGIN      
        1 ggagatgcga gagcaaaatt cagatatgtt gaaggtggtt caactcttgc tctgtatgga
       61 cttgagtggg caggtgtaga tgacgagaca ggaaagaatg tctggtatct taataacgat
      121 gctacgcctg atttgactgt caatggacgt ccggcaacat tttcctattc caaggcgagt
      181 gaggtgattc ttggtgatgt taatccaaaa ctatttggtg gtattaatac tgacgttaaa
      241 tggaaaaatt tttctgcagg attaaacttc acatataaaa taggtggata tacatatgat
      301 ggtgcaggaa gagacgtaac tgatgatggt tattactggg agagaatagt atctaaggat
      361 cagtacaaga acagatggac accggagcat aaagatgcaa agttacctat gcgtgcagca
      421 attgatatgg aggatgtaaa tcagaagagt agtcgtcaca tgcataaggc agattatctc
      481 aggttgaaga acataacaat aggttacagt cttccaaaga accttatcag caaggttaat
      541 atttccgatg tgaggattta ctttaatgga tcaaatcttt ggactctttc agcctatgat
      601 gtatatgacc cagaagttaa tgaatatggc tcaaggggtt gggagactcc tataagcaag
      661 acctatactt tcggtgttga atttactttc taaaataaaa ggactctaca atgaaaaaga
      721 taaatatatt ttttgcaata ttgagcattg tactgctttc atcatgcgaa ggttttcttg
      781 atgttaagcc aacaaactct gctgatgcag gtacttctat tactacagca gccgatgcta
      841 aagttataat aaacggtttg atgagtaaaa tggtctcatc caactattat gggagaaact
      901 tcttccttta cggagatgcc aaaggaggag attttgccat tcgttcacaa gggcgaggga
      961 atgatgcgct ttatgttttt aatcactcag cgacttcaaa ctcttattca ggatattggt
     1021 cacagatgta tcattgctta cttcagataa acaatcttct tgcaaacatt gaaaagattg
     1081 aggcagccgg aaaagggtca gcaactttga gtgattacaa gggacaggct cttacagcac
     1141 gtgcgctcat ctattttgat cttgtaagat tatatggcaa gccatataac atggacaaaa
     1201 attcactcgg agtaccactt gagcttactc ctcttgatgc atctgcacag cctacaagag
     1261 ccactgtaac tcaggtctat gatcagatac tcgcagatct tactgctgct gcgccactta
     1321 tgagcaaaac aaaaactttg ggatttctta attactatgc caataaagcc attcaggcca
     1381 gagtatacct ttatatggag aattatcctg cagcccttac agcagctcag gaaattatta
     1441 atagtggcaa gtattctctc tattcaaatg ccaactgggt tagttcatgg tctacagaga
     1501 atggcagcga atcgatattt gaacttgggg tatatctgag tgaagcagac ttgggaactt
     1561 catcgcttgg ttattatctt cttcgtacag gtactttaaa aggagctatg ggctggttca
     1621 tggccagtga ttcattcctt gccagactca atgaagaccc ttcagatgtg cgttggggca
     1681 ttatggacta tgatgagaca tatagtgaaa ccggcgtcaa gagatatggg tcatgtcgta
     1741 aatataccgg tgttgatctt aagggagata agggatcacc ttccgctgtg aacatcaaag
     1801 taattcgtct tt
//
LOCUS       DAOTQL010000244         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_52148_length_1808_cov_4.188249, whole genome shotgun sequence.
ACCESSION   DAOTQL010000244 DAOTQL010000000
VERSION     DAOTQL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52148_length_1808_cov_4.188249"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..659
                     /locus_tag="P5293_09145"
     CDS             141..659
                     /locus_tag="P5293_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRR50098.1"
                     /translation="MAIIRKLNDIEPKIGERCFIAENAVIIGDVIIGDDCSIWYGAVL
                     RGDVNPIRIGNRVNIQDGAVLHTLYKKSTVEIGNDVSVGHNAIVHGAKVGNNVLVGMG
                     AVLMDNAVIHDNTIIAAGAVVLSNGELESGVYAGVPAKKVKSESEDIGIAAHKNAEGY
                     MMYKEWYLKEEN"
     gene            682..>1808
                     /locus_tag="P5293_09150"
     CDS             682..>1808
                     /locus_tag="P5293_09150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRR50099.1"
                     /translation="MKKLLLFMAVIGLVACSGQKKNTYKIEGTITGDSITATKVYLKN
                     FSRTNPIKDTADLIKGKFVFKGSVTTPDRYMITFDSIKGRIVFFLENAKYTIEADASD
                     LSKAVVKGGVTNDLITALNAKKDSVAQAYKMSDLIKEYNNAATNEARKKEIDSIYEQM
                     QKEINVLDSLFYVNNPTSPYTLIQYVQNLEKFSIEDAEAKLANFKSLPEFAGNTYVTE
                     LETSIATLKSLQPGNKAPEFTLSDPEGKQISLSSIYSQNKITMIDFWAGWCSPCRNFN
                     PELVKIYKEMHKAGFGIIGVSLDQDAELWKKAIEEDKLEWPQVSELKYWDSEVAKMYH
                     VKYIPQNIFIDQQGNIIKRQIPEEEILGFIKGYLEGLKGKKT"
BASE COUNT          620 a          275 c          393 g          520 t
ORIGIN      
        1 taagtaatta tgacctcaat ccttataatt atgcaatatt agatacagtt ttgctaattg
       61 cataaattac tctataaaca tcgtctttaa ttgttaatta gttcaattat tattatattt
      121 gtttttctag agatataatt atggcaatta tcagaaaact gaacgatatt gaaccaaaga
      181 ttggagaaag atgctttata gctgaaaatg ctgtgatcat cggtgatgtg ataattggtg
      241 atgactgcag tatatggtac ggagctgttt taagagggga tgtcaatcct atccgaattg
      301 ggaatagagt taacattcag gatggagctg ttctgcatac tctttacaag aaatccacag
      361 ttgaaatagg caacgatgtc tcagtagggc acaacgcaat tgttcatgga gctaaagtgg
      421 gcaataatgt gctggttggt atgggagctg tacttatgga caatgctgtt attcatgata
      481 acactattat tgctgcaggt gcagtggttt tgagtaatgg agaactggag tccggagttt
      541 acgcgggagt tcctgcaaag aaagttaaaa gtgaatcaga agatatagga attgctgcac
      601 acaagaatgc agagggctat atgatgtaca aagagtggta tttaaaagaa gaaaattgat
      661 attaaccaaa aaaccggcat tatgaaaaaa ctgttattgt tcatggctgt aataggcctg
      721 gtggcctgtt cgggacaaaa gaaaaacact tacaaaatcg aggggacaat tacaggtgac
      781 agtataactg ctaccaaagt ttacctgaaa aacttttcaa gaactaatcc gattaaggac
      841 accgcagatt tgattaaggg taagttcgta tttaaaggga gtgttacaac tcctgaccgt
      901 tatatgatta catttgacag tattaagggt agaattgtat ttttccttga aaatgccaag
      961 tacactattg aagctgatgc aagtgattta agcaaagctg tcgttaaggg aggagttacc
     1021 aatgatctca taacagcatt aaatgccaaa aaggattcag tagcgcaagc atataagatg
     1081 agtgatttga ttaaggaata caataatgct gctacaaatg aggcacgcaa gaaagagatt
     1141 gattctatct atgagcagat gcagaaagag ataaatgtac ttgattctct tttctatgtg
     1201 aacaatccaa cttcgcctta tactttgatt cagtatgttc aaaatcttga aaaattctca
     1261 atcgaggatg cagaggctaa acttgctaac tttaagtctc ttccggaatt tgctggaaac
     1321 acttatgtga cagaacttga aacatctatt gcaaccctta agagtctgca acccggaaac
     1381 aaagctcctg aattcacttt gagcgatcct gaaggcaagc agatttcatt gtcaagcatt
     1441 tacagtcaga acaaaatcac aatgattgat ttctgggcag gatggtgtag tccttgccgt
     1501 aactttaacc cagaactggt aaaaatatac aaggagatgc ataaagccgg gtttggaatc
     1561 attggtgtgt cacttgacca ggatgcggag ttatggaaaa aggctattga agaagacaaa
     1621 cttgagtggc ctcaagtttc agaattgaaa tattgggata gtgaagttgc gaaaatgtat
     1681 catgtaaaat atattcctca aaacattttc atagatcagc agggcaacat tattaaacgc
     1741 cagattcctg aggaagagat acttggtttt attaaaggat atctcgaagg attaaagggc
     1801 aaaaagac
//
LOCUS       DAOTQL010000245         1806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_52230_length_1806_cov_4.715020, whole genome shotgun sequence.
ACCESSION   DAOTQL010000245 DAOTQL010000000
VERSION     DAOTQL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52230_length_1806_cov_4.715020"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /gene="hcp"
                     /locus_tag="P5293_09155"
                     /gene_synonym="priS"
     CDS             complement(<1..1560)
                     /gene="hcp"
                     /locus_tag="P5293_09155"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRR50100.1"
                     /translation="MFCFQCQETSKNQGCTLQGVCGKTADVANLQDLLIYLCKGISHF
                     SHPLRSMGYESAEVNKFITDSLFMTITNANFDKDRFVKRIREALVLRDNLRSLYVEKG
                     GKIEEIKFEGAFWSSYDVAVMEEKAGQVGVLSTENEDIRSLRELTVYGLKGMAAYAEH
                     AFNLGMEDVDVYAFMQKALVAVTDDSLSADELVSLVLETGAHGVKVMALLDKANTTKY
                     GNPEITKVNIGVGKNPGILISGHDLRDMEDLLKQTEGTGVDVYTHSEMLPAHYYPAFK
                     KYSHFVGNYGSSWWKQTQDFETFNGPILFTTNCIVPPRSNSTYRDRVYTTGAAGFPGF
                     KHIEDRKEGGQKDFSEIIELAKKCQPPVEIERGEIVGGFAHEQVFVLADKVVDAVKSG
                     AIKKFFVMAGCDGRMKDRSYYTDFAAALPKDTVILTAGCAKYRYNKLGLGDIGGIPRV
                     LDAGQCNDSYSLAVIAMKLKEIFNANDINDLPIAYNIAWYEQKAVIVLLALLSLGVKN
                     IHLGPTLPGFLS"
BASE COUNT          567 a          411 c          278 g          550 t
ORIGIN      
        1 agaaagaaaa ccagggagtg taggaccaag gtgaatgttc ttaacaccaa gagagagaag
       61 tgcaagcagc acaatcacag ctttttgttc ataccaggcg atgttgtaag caattggtag
      121 atcattaatg tcatttgcat taaaaatctc ctttaatttc atggcaatta cagccagcga
      181 ataagagtca ttgcactggc ctgcatcaag tacacgtggt atcccaccaa tatctccaag
      241 tccaagttta ttataacgat attttgcaca accagcagta agtatcacag tatccttcgg
      301 aagtgcggca gcaaaatctg tataataact cctgtccttc atcctgccat cgcaacctgc
      361 cattacaaaa aactttttaa ttgctcctga cttgactgca tccaccacct tatcagcaag
      421 aacaaatacc tgctcatggg caaatccccc tactatctct cccctttcaa tctcaaccgg
      481 aggctgacat ttttttgcaa gttcaataat ttcagagaaa tccttttgac caccttcctt
      541 tctatcttca atatgtttga aacccggaaa accagcagct ccggttgtat atactctgtc
      601 tctatatgta gagttagacc ttggaggaac aatacagttg gttgtaaaaa gaataggtcc
      661 gttgaaagtt tcaaaatcct gtgtctgttt ccaccatgaa cttccataat ttcccacaaa
      721 atgactgtac tttttaaaag caggatagta gtgtgccggt agcatttcgg agtgagtata
      781 tacatctact cctgttccct ctgtctgttt caacagatct tccatatccc tcaaatcgtg
      841 gccagaaata agaataccag gatttttccc aacgccaata ttaaccttag taatctcagg
      901 attcccgtac ttagtagtat ttgccttatc aagcaaagcc ataaccttta cgccatgagc
      961 acctgtctcc agtacaagag agactagttc atctgctgat aatgaatcat cagtaactgc
     1021 caccaatgcc ttttgcataa aagcatatac atctacatct tccattccaa gattaaacgc
     1081 atgttcagca taagcagcca ttcccttcaa accgtaaaca gtcaactctc ttagtgaacg
     1141 aatgtcctca ttctccgtag agagcacacc cacctggcct gccttttctt ccataacagc
     1201 cacatcataa gagctccaaa aagctccttc aaatttaatc tcttcaattt ttcctccttt
     1261 ttccacatac aaagacctca gattgtctct taatactaat gcctccctaa tccttttaac
     1321 aaatctatct ttatcaaaat tggcattagt aatagtcata aaaagagagt ctgtaatgaa
     1381 cttgttaact tcagcactct catagcccat acttctcaaa ggatgggaaa aatgagagat
     1441 ccccttacaa aggtatatca gcaaatcttg caaatttgct acatcggctg tcttgccaca
     1501 tactccctga agtgtgcagc cttggttttt tgatgtctcc tgacattgaa aacaaaacat
     1561 cttattcata ataattgtat taagttattt taatttaatt tattcaaatg tcgaaagtta
     1621 ttttacagaa atgtgtaata attgttacac atgtgaaaat attgtatttt tgaattaaac
     1681 attttaatca aacattttca cagactgtgc aatttgaaaa caaccaagag agtactaagt
     1741 ttaatcttct cggaaagtca tatgacaact ttcaagaagc tattcctgaa ggtgtagagt
     1801 attcgg
//
LOCUS       DAOTQL010000246         1795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_52722_length_1795_cov_3.474138, whole genome shotgun sequence.
ACCESSION   DAOTQL010000246 DAOTQL010000000
VERSION     DAOTQL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52722_length_1795_cov_3.474138"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="P5293_09160"
     CDS             <1..695
                     /locus_tag="P5293_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRR50101.1"
                     /translation="LYLIPSPVSDGEINEVIPEGVICKILPLRVFVVEEVRSARRFLS
                     KCGFKGKIDELLFLELNEHTAVSLIDSYINLLVEGKDVGLISEAGLPAVADPGALLVA
                     AAHREGIRVIPLVGPSSLMLALMASGMNGQSFAFVGYLPVKPEERRSEIKRLEKLSDR
                     TGQSQIIIETPYRNNALFSDLVQVCSPSKLICVASAITSQEESIRTLTAFDWGNEKEK
                     VDLNKKPCVFII"
     gene            728..1093
                     /gene="folB"
                     /locus_tag="P5293_09165"
     CDS             728..1093
                     /gene="folB"
                     /locus_tag="P5293_09165"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRR50102.1"
                     /translation="MKTESCDYIELTGMEFFSFHGHYQEEQIVGNRYIVDLRVSTDFT
                     KAGLSDDLSDTIDYQELYKIVANVMSTPSKLLESVAKKILDDIRDRYPDVSGATVSIA
                     KLNPALGGQVSAAKLVMSF"
     gene            1099..>1795
                     /locus_tag="P5293_09170"
     CDS             1099..>1795
                     /locus_tag="P5293_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRR50103.1"
                     /translation="MDSEFGVKGEKVSKRVAFLTLGCKLNYSESSSLARIFVDNGYER
                     VSSSAIADVYVVNTCSVTEHADKKCRSAIRKLHRQNPDAIIAVTGCYAQLKPKEILDI
                     EGVDLVIGTDQKQDVFLRVSQLVSEKHNIKNISSSSKKEFISDIHTIFPAYSSDDRTR
                     SFLKVQDGCDYHCAYCTVPLARGKSRNLPVDFLVKEANEIASKGIKEIVLTGVNTGDF
                     GKSTGENFLDLLKE"
BASE COUNT          544 a          272 c          414 g          565 t
ORIGIN      
        1 gtttatatct tattccatct cctgttagtg atggtgaaat caacgaagta attccggagg
       61 gagtcatttg taaaatactg cctttgagag tttttgtggt tgaggaagtc cgaagtgcaa
      121 gaaggttcct ttcaaaatgt ggttttaagg gaaaaataga cgagttattg tttcttgaat
      181 tgaacgagca tacagctgtt tcattaattg atagttatat aaatctatta gttgagggga
      241 aggacgttgg gttaatcagt gaagccgggt tacctgccgt ggctgatccc ggagcactgc
      301 tggtagcagc agcccataga gagggaatca gggtaattcc attagttgga ccgtcctcct
      361 taatgcttgc tttgatggct tcaggaatga acggacaatc ttttgcattt gtgggatatt
      421 tgccggtgaa gcctgaagag agaaggtcgg agataaaaag acttgaaaaa ctttctgaca
      481 gaaccggaca aagtcagata ataattgaaa ctccgtatag aaataatgct cttttttctg
      541 atctggtgca ggtatgttct ccttcaaagt taatttgcgt ggcttctgca attacttcgc
      601 aagaagaatc tattcgtacc ttaacggctt ttgactgggg aaatgaaaag gaaaaggttg
      661 atttaaacaa gaaaccttgt gtctttatta tatgattatt atattcttta caataaataa
      721 tttttgaatg aaaacagaat cttgtgatta tattgagctt accgggatgg agtttttttc
      781 gtttcatgga cattaccaag aggagcagat agttggtaat cgttatattg tagatttgag
      841 agtgagcaca gattttacaa aagcgggctt aagtgatgac ctgtctgata caattgacta
      901 ccaggaactc tataaaattg tggcaaatgt gatgtctact ccttcaaaac ttttggagag
      961 cgttgccaaa aaaattcttg acgacataag agacagatat ccggatgttt caggtgcaac
     1021 cgtttctatt gcaaagttaa atccggctct tggaggtcag gttagtgctg caaaattggt
     1081 tatgtcgttt taatagagat ggatagtgaa tttggggtaa aaggggaaaa agtctccaaa
     1141 cgtgtggcat ttcttacgct cggatgtaaa ttgaattatt ctgagagttc ttctcttgca
     1201 agaatttttg ttgacaatgg ttatgaaaga gtttcttcgt cggcaatagc agatgtgtat
     1261 gttgttaata catgttcagt tacagagcat gccgataaaa aatgccgcag tgccatcaga
     1321 aagcttcatc gccaaaatcc tgatgcaatt attgcagtta ctggctgtta tgctcagttg
     1381 aaaccaaaag agattcttga tattgaaggt gttgacttag ttattggcac agatcagaaa
     1441 caggatgttt tcttaagagt ttctcaatta gttagcgaaa agcataatat taaaaatata
     1501 tcgagcagtt caaaaaaaga gtttataagt gatatacata ccatatttcc tgcctattca
     1561 tctgatgaca ggacaaggtc ttttttaaaa gttcaggatg gttgtgacta tcactgtgct
     1621 tactgtacag ttcccctcgc aagaggtaaa agcagaaatt tgcctgttga ctttctggtg
     1681 aaagaggcta atgagatagc ctctaaaggc ataaaggaga ttgttttgac cggggttaat
     1741 accggtgatt ttggaaaaag tactggagag aattttctcg atttgctcaa ggaac
//
LOCUS       DAOTQL010000247         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_53096_length_1787_cov_3.354503, whole genome shotgun sequence.
ACCESSION   DAOTQL010000247 DAOTQL010000000
VERSION     DAOTQL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53096_length_1787_cov_3.354503"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1787
                     /locus_tag="P5293_09175"
     CDS             39..>1787
                     /locus_tag="P5293_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRR50104.1"
                     /translation="MQRKISNLEFQGLSEQEVKNRQTREGFNELPSSKRKGVFKIIAG
                     IFTEPMFILLVTCGLLYLFLGDVQEGIMLMSFVVFVMGIEYFQERKTEKALDALKDLA
                     SPRALVIRGGEVKRIAGREVVRGDIVVVQEGDRIPADGKVLNSVNLMIDESLLTGESV
                     PVRKREWIEGDIYSIPGGDDLPYLFSGSMVVQGNGVFEVTAIGHSTEIGKIGKALESV
                     KEEPTKLKREMGILVKRLAILGLLLCVLVIVIYTLTRGDLLQGFLAGITLAMAMLPEE
                     FPVVLTIFMALGAWRIAKNSVLTRKSSAIETLGSATVLCTDKTGTLTQNKMTISALYN
                     GNNLFKLSNATFFPEEFHEIIEYGLLASQVRPFDPMEKAIEALSEKFLKESEHIHSDW
                     KMVKEYPLSREMLAMSRVFESPESDIKVIATKGAPEAIFDLCHLDSDNVARLSAVVKE
                     MASDGLRVLGVAKAVLAPETLPHVQHDFDFEFVGLIGLHDPIRENVPEAVRECYRAGI
                     RVIMITGDYPVTAMNIAKEIGLDNYGEYITGNELMEMSDDELAKRIKNVNIFARVVPE
                     QKLKIINALKRNGEIVA"
BASE COUNT          564 a          257 c          438 g          528 t
ORIGIN      
        1 aattgttgaa aaaatagttg gttgaataat aaaattctat gcaaagaaag atatcaaatc
       61 tcgaatttca aggactgagc gaacaagagg tcaaaaacag gcagacaaga gagggattta
      121 atgaacttcc atcttccaaa agaaaaggag ttttcaaaat tattgcaggc atttttacgg
      181 aaccaatgtt tatcttactg gttacgtgcg gcttacttta tctttttctt ggagatgttc
      241 aggagggtat catgttaatg tcatttgtgg tctttgtgat ggggatagag tattttcaag
      301 agagaaagac tgagaaggca ctggatgctt tgaaagatct tgcaagtccc agagctttgg
      361 ttataagggg tggagaggtt aaaagaattg ccggaagaga ggttgtgaga ggagacatag
      421 tggttgttca ggagggagac agaatacctg cagatggtaa ggtacttaat agtgtcaatc
      481 ttatgataga tgaatctcta ttaacaggag aatcagtacc ggttagaaaa cgagagtgga
      541 tagaaggaga tatttattct atacccggag gggatgactt accttatctt ttttcagggt
      601 cgatggttgt tcagggaaat ggtgtgtttg aagttacggc cattggacac tctactgaga
      661 tagggaaaat aggcaaagca ctggaatctg tgaaggaaga acctaccaaa ctcaaacgtg
      721 aaatggggat tctggttaaa agattggcga tattaggact attgttatgt gttttagtaa
      781 ttgtcatata tacattgaca agaggcgatt tacttcaagg atttcttgca ggtattacac
      841 tggccatggc tatgttgcct gaggaattcc cagtagttct tactattttc atggctcttg
      901 gtgcatggag gattgcaaaa aattcagtac ttacaaggaa atcatcggct attgaaactc
      961 ttggctcagc aacagttctt tgtactgaca aaacaggaac tcttacgcag aataaaatga
     1021 ccatcagtgc tttgtataac ggaaataatt tgtttaaact ttccaatgct actttttttc
     1081 ccgaagagtt tcatgagata attgaatatg gtcttcttgc aagccaggtt aggcctttcg
     1141 atcccatgga aaaggccatt gaagctctaa gtgagaaatt tctcaaggaa agcgagcata
     1201 tacattcaga ttggaaaatg gtaaaggagt atcctttatc cagagagatg ctggcaatgt
     1261 cgagggtttt tgaaagtcct gagtcggata ttaaagttat tgcgacaaaa ggtgctcctg
     1321 aggctatttt tgatctttgc catcttgata gtgacaatgt ggccagatta agcgccgttg
     1381 tgaaggaaat ggcttcagat ggcttgagag ttcttggtgt tgcaaaggca gtccttgcac
     1441 ctgaaacatt acctcatgtc caacatgatt ttgattttga atttgttggt ctcatagggc
     1501 ttcatgatcc tattcgagaa aatgttcctg aagcagtgag agagtgttat agagcgggta
     1561 taagagtgat aatgattaca ggagattatc cggtaacagc catgaatatt gctaaagaga
     1621 ttgggcttga caattacggt gaatacatta ccggaaatga gttgatggaa atgagtgatg
     1681 atgaacttgc caaaaggata aagaatgtta atatttttgc cagagtggtg ccagagcaaa
     1741 aattgaaaat tataaatgca cttaaaagaa atggtgaaat tgtagca
//
LOCUS       DAOTQL010000248         1772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_53770_length_1772_cov_4.306348, whole genome shotgun sequence.
ACCESSION   DAOTQL010000248 DAOTQL010000000
VERSION     DAOTQL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53770_length_1772_cov_4.306348"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="P5293_09180"
     CDS             <1..895
                     /locus_tag="P5293_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50105.1"
                     /translation="LLGTTNYLFEVIREREYRKIGYELEKNKISNAIIRVPATIQWNR
                     INFKLKDHEEIKNFIKPDIIVTLIDAEWLIRERFEKAPENNLFLNRIKEQKHTIRDIL
                     SWMNEEVSLSEDWAAYMGVRHYVIPTGQDPRSISKLIKFKNVPSFYASYSMTHCDKEA
                     RDEINNVIKRLSKYGLVLDPQAIEIGFNYDSVEDKEATYAYTVHRDLHWFVGKVDAVV
                     AIHPYAERPPLSTGMMDELGHARDYLKKRYMIFPPRNLSPFTTDSYIEKGHIFTNADE
                     FFLCLEAEGFKPLSEGTKTKK"
     gene            911..>1772
                     /locus_tag="P5293_09185"
     CDS             911..>1772
                     /locus_tag="P5293_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50106.1"
                     /translation="MPDKELEVSCPGCSAIFNVPAELAGEIAECSECGAVFEIPKPEE
                     SPQEPGTETGAIKGKITEESETATNTVKLSRTSIGMIPSLKDSFTFGDPVKSTTPPPP
                     RPGSGPTFQQPTQVQKPASTPTQSGFPIPPARQQPIQQTTPPVESPQFKTQMRHPSPP
                     PQQPHTPPVSSPPPPPPSSASSAQKMTFKKPTVPSQTQAPHQSTPPLQQNIQLPPWTR
                     IQLRPDEQLYGFREITGNPVGTAFGASFPILISLIAVFMAKSDAVISYIIVVAIWLVT
                     FVTIALISAKS"
BASE COUNT          641 a          387 c          293 g          451 t
ORIGIN      
        1 gctcctcggg actacaaatt atctttttga agtaatccga gaaagagaat accgcaaaat
       61 cggatatgaa cttgaaaaaa ataaaatttc aaatgcaata atcagagtac ctgctacaat
      121 tcaatggaat agaataaatt tcaaactaaa agaccatgaa gaaataaaaa acttcattaa
      181 gccggacata atcgtaaccc tcatagacgc tgaatggtta ataagagaga gatttgaaaa
      241 agcaccagaa aataatctct tcctgaacag aataaaagaa caaaaacaca ctataagaga
      301 tatattaagc tggatgaatg aagaagtaag cttatcagaa gattgggctg cttatatggg
      361 agtcagacac tatgttatac caacggggca agatccccgt tcaatttcaa aactcataaa
      421 atttaaaaat gtcccttctt tttacgcttc ctattcaatg actcattgtg ataaagaagc
      481 aagagatgag ataaataatg taataaaaag gctttccaaa tatggtcttg tcctggatcc
      541 acaagcaata gaaattggat tcaattatga ttccgttgaa gataaagaag ctacatacgc
      601 ttatactgtt catcgcgacc ttcattggtt cgttggaaaa gtagatgctg tagtggcaat
      661 acatccttat gctgagaggc cccctctttc aacaggtatg atggatgaac tcggacacgc
      721 aagggattat cttaaaaaaa gatatatgat atttcctcca agaaatctca gtcccttcac
      781 aactgacagt tatatagaaa aaggccatat ttttactaat gcagatgaat ttttcctatg
      841 ccttgaggct gaaggattta aacctctatc agaaggaaca aaaacaaaga aataaaaggg
      901 agtatcgaaa atgcctgata aagaacttga agtatcatgt ccaggttgtt ctgctatatt
      961 taatgttcct gccgaattag ccggggaaat tgcagaatgt tcagagtgtg gagcagtatt
     1021 tgaaatacca aaaccagaag aaagtcccca ggagccagga actgaaacag gcgcaataaa
     1081 aggtaaaatt actgaagaaa gtgaaacagc tacaaataca gtaaaacttt caagaacaag
     1141 catcggaatg ataccttctc ttaaagattc atttactttt ggcgatcctg tcaaatcaac
     1201 aacccctccc ccccccagac caggctcagg ccctacattt caacaaccta ctcaagtgca
     1261 aaagccagca agtactccaa ctcaatctgg atttccaata ccacctgccc gtcaacaacc
     1321 aatacaacaa acaactccac ctgtagagtc acctcaattc aaaacacaga tgagacatcc
     1381 atcgccaccg ccacaacaac cgcatactcc tccagtatca tcccctcctc caccaccacc
     1441 atcttcagca tcttcagcac aaaagatgac atttaaaaaa cctactgttc cttcacaaac
     1501 acaggctcca catcaatcaa caccaccatt gcaacaaaat atccaattac caccatggac
     1561 gagaattcaa cttcgtccgg atgaacaact ttatggattt agagaaataa ctggaaatcc
     1621 cgtaggaact gcttttggtg cttcattccc aattttaatc tctctcatcg ccgtttttat
     1681 ggcaaagtcg gacgcagtaa tttcatatat catagtagtt gctatctggt tagttacctt
     1741 cgttacaatt gctttgatat ctgcaaaatc ta
//
LOCUS       DAOTQL010000249         1739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_55309_length_1739_cov_3.272565, whole genome shotgun sequence.
ACCESSION   DAOTQL010000249 DAOTQL010000000
VERSION     DAOTQL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55309_length_1739_cov_3.272565"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="P5293_09190"
     CDS             complement(<1..416)
                     /locus_tag="P5293_09190"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRR50107.1"
                     /translation="MQCTLSKVVKFEGKGLHTGLFVHMEIHPASINHGIVFRRVDLPG
                     EPEVPAVAEYTVSTQRGTTIGKGDAQVSTIEHIMATLFALGIDNALIKIDGPEVPIID
                     GSALGFANRLSESGLEKQNAPSNYYTPERTIYYKDD"
     gene            complement(421..1428)
                     /gene="lpxD"
                     /locus_tag="P5293_09195"
     CDS             complement(421..1428)
                     /gene="lpxD"
                     /locus_tag="P5293_09195"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRR50108.1"
                     /translation="MKKQMMITAGTIAKLVHGEIFGDPETVVSSITKIEQSKRGSISF
                     YANQKYEKYLYNTKASIILINKDFIPSAPLPAPCIIKVDNAYQCMPVLLDLFNTIKAK
                     NKRGRGFFTKISWSAKIGKNAYIGSHSYVGKSTVIGTGSQVYPHSYIGDNVTIGENTI
                     IYPGVKIYAACKVGSNCIIHANAVIGADGFGFSPDSNGVYNKVPQIGNVVIEDNCEIG
                     ANTTIDRATMGSTIIRKGVKMDNLIQIAHNCDIGENTVIAAQAGIAGSTVIGKNCIIG
                     GQVGIGDHLVIADNTTIVSQAGVLSNIKVPGTILAGSPAFDYKKFMKSYALFRDLNKK
                     K"
     gene            complement(1425..>1739)
                     /locus_tag="P5293_09200"
     CDS             complement(1425..>1739)
                     /locus_tag="P5293_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50109.1"
                     /translation="ISAIKQWQYCDDKILSLLSRNLSMRRLPKIILSSEPINPELCTG
                     EDYLVQCGEVVNEGYDIEGEFIGIMSKDGIVKNIYDISDMLSAKAFSQVTKKYFLCTA
                     RL"
BASE COUNT          521 a          368 c          282 g          568 t
ORIGIN      
        1 ttgtcatctt tgtaataaat tgtacgttca ggagtataat agtttgaagg tgcattttgc
       61 ttttccaaac cactctcaga caacctattt gcaaacccca atgcacttcc atcaataatt
      121 ggaacttcag ggccatctat ctttatgagt gcattgtcta ttccaagggc aaacagagtc
      181 gccataatat gctctatggt agatacttga gcatcgcctt ttccaattgt ggtgcctcgt
      241 tgggtagaaa cagtatattc agcaacggca ggcacttcag gttctcctgg gaggtcaact
      301 cttctaaaca caatgccatg atttatcgaa gcaggatgga tttccatatg cacaaaaagt
      361 ccggtatgta atcctttccc ctcaaatttt acaacctttg acagagtaca ctgcatagca
      421 ttattttttt ttgttaagat ccctgaaaag tgcatatgac ttcatgaact ttttgtagtc
      481 aaaagcagga gagccagcca aaattgtgcc tggaacttta atattgctaa gaactccagc
      541 ctgggaaaca atagttgtgt tgtctgcaat gacaagatga tcacctatgc cgacttgccc
      601 acctattata caatttttac ctattacagt agaacctgca attcctgcct gagcagcaat
      661 aacagtgttt tctccaatat cacaattatg tgctatctga ataaggttgt ccatcttgac
      721 tcccttccta attattgttg accccattgt tgctctgtca atggtagtat ttgctccaat
      781 ttcacaattg tcttcgatga ctacattccc aatctgaggt actttattgt agactccatt
      841 actatcagga gaaaatccaa acccgtctgc tcctataact gcgtttgcat gaattataca
      901 atttgaacct accttgcaag cagcataaat cttaactccc ggataaataa tagtattctc
      961 cccaatagtg acattatcac caatataaga atgtgggtaa acttgagacc ctgtaccaat
     1021 cactgtcgac ttaccaacat aagaatgtga acctatatat gcatttttcc caatttttgc
     1081 actccaggaa atcttcgtaa aaaaacctct tcctcttttg tttttagcct taatagtatt
     1141 gaagagatca agtaaaaccg gcatacactg atatgcatta tcaaccttaa ttatacacgg
     1201 agctggtaag ggggcagaag ggataaaatc cttgttaatc agtataatac ttgccttagt
     1261 attataaaga tatttttcat atttttggtt agcgtaaaaa cttattgagc ctcgcttgct
     1321 ttgttcaatc tttgtgattg aacttactac tgtttcagga tctccaaaaa tttctccatg
     1381 aaccaactta gcaatagttc cagctgttat catcatctgt tttttcataa tctggctgtg
     1441 caaagaaagt atttttttgt gacttgagaa aaagccttag ctgaaagcat gtccgagata
     1501 tcatatatgt ttttcacaat tccatcttta gacataatgc caataaattc accttcaata
     1561 tcatatcctt catttacaac ctcaccgcac tggacaagat aatcttcgcc tgtacataat
     1621 tccggattaa ttggctctga agaaaggata atttttggca atcgacgcat tgatagattt
     1681 ctactcaata gcgacaaaat tttgtcatca cagtactgcc actgtttaat agctgaaat
//
LOCUS       DAOTQL010000250         1736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_55450_length_1736_cov_5.509816, whole genome shotgun sequence.
ACCESSION   DAOTQL010000250 DAOTQL010000000
VERSION     DAOTQL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55450_length_1736_cov_5.509816"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1111)
                     /locus_tag="P5293_09205"
     CDS             complement(311..1111)
                     /locus_tag="P5293_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRR50110.1"
                     /translation="MKRLMIAIVVMTMLVTVIGGVLLVSADADVSVTVDGNKVVFPDA
                     KPFIDENGRTLIPVRFVTEDLGATVEWNAQDREVYITKDGVNIMIRIGQEIILVNGRT
                     KTMDTKAIIKEDRTYVPIRYVAEELGATVGWDASTRTVIITTVGGSTDPTPTPTPSKV
                     SSVTPTKDPITGWIKVEENVADTEFSMSILWDPDNEILNKRYDAAERLLAERYGDEIA
                     KEVFDYVRLKQTRSYKLEMKKFRLNNQIVTASGSGIAVDVYVWKEGVL"
     gene            complement(1188..1322)
                     /locus_tag="P5293_09210"
     CDS             complement(1188..1322)
                     /locus_tag="P5293_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50111.1"
                     /translation="MVSIHKDRIELEDALDVESDKYINKAASFKGKIEKEKTIEILDR
                     "
     gene            complement(1295..>1736)
                     /locus_tag="P5293_09215"
     CDS             complement(1295..>1736)
                     /locus_tag="P5293_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50112.1"
                     /translation="ELNATESGIIYLGMTMDELWNALEIANIDRKKLTRVEITDHPAS
                     WHYGNIYLESVDCSFEFNKENRLHSIRIEKTIPGKGKFIPTKLGLEIGDSIEKMEQLY
                     GTEYEKSIRGDNEIGFSYDMGEYTFWGIFKDNELILWSVFTKIE"
BASE COUNT          453 a          421 c          208 g          654 t
ORIGIN      
        1 ttctctatat cttttaatgc ctctgtatct acagaaatta gtgacattga aaatcgcccc
       61 ctttgcaatc ctattgtaac caatttggaa attattatat tttattgggt tattgattac
      121 aatagatttt gacaaatggt acatttcatt ggacaaaaga cattgttttt ctatttttta
      181 cctttcgaat ttgattcctc gtttgcaatt ttcaccaaat caggtcttgg aatatcttcc
      241 gccatagcaa tactcttgca taaaaacata cacataataa aaccagtcaa agcaaaagct
      301 ttctcattcc ttaaagtacc ccttctttcc acacatatac atccacagca atacctgaac
      361 cagaagccgt tacaatctgg ttattcagcc taaatttctt catctctaat ttataacttc
      421 gtgtttgctt taacctcaca taatcaaaca cttcttttgc aatctcatct ccatacctct
      481 ccgccaacaa tctctccgct gcatcgtacc tcttgtttaa aatctcgttg tccggatccc
      541 atagaattga catagaaaac tctgtatctg ctacattttc ctcaaccttt atccatccgg
      601 taataggatc ctttgtcggt gtaaccgatg atacctttga aggtgtcgga gtaggtgtcg
      661 gatccgtgct tccgcctact gtcgttataa ttacagtcct tgtgcttgca tcccagccta
      721 ctgttgctcc caactcttct gccacatacc ttatcggtac atatgttctg tcctctttta
      781 ttatcgcctt tgtatccatt gtcttggttc tgccgtttac cagtattatc tcctggccta
      841 ttcttatcat tatatttact ccgtcttttg ttatgtacac ttctctgtct tgtgcattcc
      901 actccactgt tgctcccaaa tcctccgtta cgaatcttac cggtatcaat gttctgccat
      961 tttcatctat aaacggcttc gcatcaggaa ataccacctt attaccatct accgttacac
     1021 ttacatctgc atcggcactt accaataata ctccgcctat tactgtcacc agcattgtca
     1081 ttacaactat tgctatcatt aatcttttca tatgaattca cctttttatg tctaattttt
     1141 tattaattta tatacatgga tatttggtaa ttcaccagac ttacattcta tctgtccaga
     1201 atttcaattg ttttctcctt ttctattttc cctttaaagc tcgcagcctt attgatatac
     1261 ttgtcacttt ctacatctaa cgcatcctct agttctattc tatctttgtg aatactgacc
     1321 ataatatcag ttcattatct ttaaatattc cccaaaaggt atattccccc atgtcatatg
     1381 aaaaaccaat ttcattatca cctctgatac ttttctcata ttctgtacca taaagctgtt
     1441 ccattttctc tattgaatca ccaatttcca agccaagctt ggtaggaata aattttcctt
     1501 tacccggtat agttttttct attcttatag aatgaagcct gttttcttta ttgaactcaa
     1561 aactacaatc tacagactct aaatatatgt ttccatagtg ccatgaagcc ggatggtcag
     1621 ttatctcaac ccttgtcaac tttttcctat ctatgtttgc aatttctaag gcattccaaa
     1681 gttcatccat tgtcatacct aagtaaataa ttccgctttc tgtggcattt aattca
//
LOCUS       DAOTQL010000251         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_55592_length_1733_cov_5.230036, whole genome shotgun sequence.
ACCESSION   DAOTQL010000251 DAOTQL010000000
VERSION     DAOTQL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55592_length_1733_cov_5.230036"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..933)
                     /gene="mazG"
                     /locus_tag="P5293_09220"
     CDS             complement(106..933)
                     /gene="mazG"
                     /locus_tag="P5293_09220"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRR50113.1"
                     /translation="MKKDRNEEIKAFVRLLDIMDELREKCPWDREQTAESLRPLTIEE
                     TYELSDAIINSDDYNISKELGDILLHIVFYAKIASEKGSFDISDVINRCCDKLIYRHP
                     HVFGETQVSGAGEVIKNWEQLKRKEKDGNKTILSGVPSSLPSIVKAYRMQDKVRAVGF
                     DWEKREEVWEKVREEIAEFEEELKNNAAGDLEKETENNVDCNLEGEFGDLLFSLINAA
                     RLYNINPDTALEMTNRKFFKRFGYIESETTKRGLSIKSLSLEEMDRLWNEAKNLENR"
     gene            complement(947..>1733)
                     /locus_tag="P5293_09225"
     CDS             complement(947..>1733)
                     /locus_tag="P5293_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRR50114.1"
                     /translation="FRLMTIVTLTSDWSKGDYYTGALKGMLLSLDGGIEVVEISKSIT
                     PFDVLQEVFILKNTYRFFPVGSIHLMGVMSEPSPDVDMIVVYSDGHYFIGVNDGRFSL
                     LLDSVPAIAFSIVEDIVRNSFSSLGLFVKGVKTIMGNSFNENTVAAEVKIETSPQLVY
                     SEESITGRVVYIDSFGNAITNIEKTLFDKIHRGRNFTIFVAGPYSKIRKISSSYFSHD
                     PGEIFALFNSLGLLEIAVYQGNIAVLENIDTSTEIKIRFENSY"
BASE COUNT          547 a          415 c          243 g          528 t
ORIGIN      
        1 aagctccaac acctccaaca cccacaacaa ctactcgttt ggatgcaaag ctatctatca
       61 ctttgcgacc taacaatagt tcactccttt ccaaccaatt ctccatcatc tattttcaag
      121 atttttagct tcattccaca atctgtccat ctcttcgagt gagagagact ttatagacaa
      181 acccctttta gttgtttcgc tttcaatata gccaaagcgt ttgaaaaatt tacggtttgt
      241 catctcaaga gccgtatcag gatttatatt ataaagtctt gccgcattaa tcaaagagaa
      301 caaaagatcg ccaaattcac cctccaagtt gcaatccaca ttattttcag tttctttttc
      361 aagatcacct gcggcattat tcttaagttc ctcttcaaat tcagctatct cttcacgaac
      421 cttctcccac acctcttctc ttttctccca gtcaaaacct acagctctaa ctttgtcctg
      481 catgcggtat gccttaacaa tagatggcaa ggatgaagga actccagaaa gaatagtctt
      541 attgccatcc ttctcctttc tcttaagttg ctcccagttt tttataacct ctcccgctcc
      601 tgaaacctga gtctcaccaa aaacatgagg atgcctgtat ataagcttgt cacaacacct
      661 gttaataaca tcggaaatat cgaaactgcc cttttcagaa gcaatttttg catagaaaac
      721 aatatgaagc agtatgtctc caagctcctt gcttatatta taatcatccg aattaataat
      781 cgcatcactc aattcataag tctcctcaat agtaagaggt ctaagagatt ctgcagtctg
      841 ctccctgtcc cagggacatt tttctcttag ctcatccatt atgtcgagaa gccttacaaa
      901 agctttaatc tcctcatttc tatccttttt cattactatt tataatttaa taactattct
      961 caaatctgat tttaatctca gtagatgtat caatgttttc caaaaccgct atattaccct
     1021 ggtatacagc tatctcaagc agtcctagtg aattaaacaa agcaaaaatt tcaccgggat
     1081 catgagaaaa gtaacttgaa gatatctttc ggattttaga atatggccct gccacaaata
     1141 ttgtaaaatt tcttcccctg tggattttgt caaaaagtgt cttttcgata tttgtaatcg
     1201 cgttaccaaa agagtcaata tacacaactc tgcctgtaat cgactcttca ctgtaaacca
     1261 actgaggtga agtttcaatt ttcacctctg ctgcaacagt gttttcatta aatgagttac
     1321 ccataattgt ttttactccc tttacaaaaa gtcccaatga ggaaaaagaa ttgcgtacaa
     1381 tatcttcaac tatggaaaaa gctatagcag gcactgaatc caacaaaaga gaaaaacgtc
     1441 cgtcattgac acctatgaaa taatgaccat ccgaatacac aacaatcata tccacatccg
     1501 gggaaggctc actcattaca cccatcagat gaatagaacc tacaggaaaa aacctgtaag
     1561 tattttttag aataaacacc tcctgtaaaa catcaaaagg ggtaatagac tttgaaattt
     1621 caaccacttc aatcccgccg tcaagagata acaacattcc tttgagagca ccggtatagt
     1681 agtcaccctt gctccagtcg ctcgtaagtg taacaatagt catcagccgg aat
//
LOCUS       DAOTQL010000252         1733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_55597_length_1733_cov_3.655542, whole genome shotgun sequence.
ACCESSION   DAOTQL010000252 DAOTQL010000000
VERSION     DAOTQL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55597_length_1733_cov_3.655542"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1733)
                     /locus_tag="P5293_09230"
     CDS             complement(<1..>1733)
                     /locus_tag="P5293_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRR50115.1"
                     /translation="LERAVPHIDDGLKPVQRRILHAMKKMDDGRYNKVANIIGFTMQF
                     HPHGDASIGEALVQLGQKEYLIDCQGNWGNILTGDSAAAPRYIEARLSKFANEVLFNP
                     KTTVWMKSYDDRNLEPVNLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDASIDYL
                     NNRDFILYPDFPTGGLIDCSRYNQGLRGGVVKVRAKITKLDKRTLVITEIPFGLTTSK
                     LIESILKANDKGKIRIKKVDDNTAAGAEIVVHLANEVSPDKTIDALYAFTDCEISISP
                     NSCVIKDNYPHFIGVDEILRHNTDVTKSLLNKELEIRLSELEEAWHYTSLEKIFFEQR
                     VYKILEDKAESWDDQLDAVETELKKYESQLRKEVVHDDVLRLVEKPVRKISSFDIKQL
                     DISIKNIESEEKTVKHNLDHLVDYTIQYFSQLKKKYGAKYPRKTEICNFETIEATRVV
                     VANAKLYANKAEGFVGMDLKRDENAEYICECSDIDDIIVFLKNGKYLVTKISEKAFVG
                     KDIIHVGVFTKNDDRCIYNVVYRNGKGGETYVKRFAVTGVTRDKEYDLTQGKEGSQVL
                     WFTANPNGEAE"
BASE COUNT          482 a          384 c          315 g          552 t
ORIGIN      
        1 ctctgcctca ccatttggat tggcagtaaa ccaaagcacc tgagaaccct ccttcccctg
       61 cgtaagatca tactctttat cgcgagtgac accggtaaca gcaaatcttt tgacataagt
      121 ttcgccgcct ttcccattac gatagactac attgtaaatg catctgtcat catttttcgt
      181 aaaaacaccc acatgaatga tatccttgcc tacaaaagcc ttttcgctta ttttggtcac
      241 taaatatttt ccattcttaa gaaaaacaat aatatcgtct atatcagaac attcacagat
      301 atattcggca ttttcatccc tcttaaggtc catacctaca aaaccctcag ccttgttggc
      361 atagagtttg gcattagcga ctacaactct ggtggcttca atggtttcaa agttgcagat
      421 ttcagtcttt cgaggatatt tagcaccata tttctttttt aactgagaaa aatattgaat
      481 agtataatcc acaaggtgat cgagattgtg ttttactgtt ttctcctcac tctcaatatt
      541 cttaatagat atatcgagct gcttgatatc aaaggaagat attttcctta cgggcttttc
      601 aacaagacgt aggacatcat catgtacaac ctcctttcta agctgtgatt catacttttt
      661 aagttcagtc tcaacagcat caagctgatc atcccaactt tcagctttat cttccaatat
      721 tttatatacg cgctgttcaa aaaatatctt ctcgagagag gtgtaatgcc atgcctcttc
      781 cagttcagac aatctgattt caagctcttt gttgagaagt gactttgtga catcagtgtt
      841 gtgcctgagt atttcgtcca ctcctatgaa atgaggatag ttatccttga tgacacaact
      901 atttggagat attgatatct cgcagtcagt aaaggcataa agcgcatcaa tagtcttatc
      961 gggagatact tcgtttgcaa ggtgaaccac aatttcagcc cctgcagctg tattgtcgtc
     1021 aactttcttt attctgattt tccccttgtc atttgccttg aggatagatt ctatcaactt
     1081 ggaggttgta agtccaaaag ggatctcagt aatgaccaaa gtccttttat cgagcttcgt
     1141 aattttggct ctaaccttta ccactcctcc tctgagtccc tggttatatc tggaacaatc
     1201 aatcagacct cctgtaggga aatctggata gagtataaaa tcccgattgt tcagatagtc
     1261 tatggaggca tcaataagct cattgaaatt gtgtggaaga attttagaag caagacctac
     1321 agcaatacct tctacaccct gtgcaagcaa tagtggaaat ttgacaggaa ggtttacagg
     1381 ctccagattc ctgtcgtcat aagatttcat ccagacagtt gtctttggat tgaaaagcac
     1441 ctcatttgca aatttgctga gtcgtgcctc tatataacga ggggcagcag cagaatctcc
     1501 cgtaagaata ttaccccagt taccctgaca gtcaatgaga tattcttttt ggcccaactg
     1561 taccaatgct tcaccaatag atgcatctcc gtgtggatga aattgcattg taaagccgat
     1621 gatgttggcc actttgttgt agcggccgtc atccatcttt ttcatggcat ggagaatgcg
     1681 tctctgtaca ggcttcagac catcatcgat atgaggaacg gctctttcga gaa
//
LOCUS       DAOTQL010000253         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_58401_length_1678_cov_4.502773, whole genome shotgun sequence.
ACCESSION   DAOTQL010000253 DAOTQL010000000
VERSION     DAOTQL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58401_length_1678_cov_4.502773"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="P5293_09235"
     CDS             complement(<1..121)
                     /locus_tag="P5293_09235"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRR50116.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDVLAKDYPVVARFQGGPNAG"
     gene            complement(149..571)
                     /locus_tag="P5293_09240"
     CDS             complement(149..571)
                     /locus_tag="P5293_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRR50117.1"
                     /translation="MSLNKVMLIGNVGKDPEIRHLDNDNIVAKFTLATTEKYRDRDGK
                     WQEQTEWHNIVCWRSLAERAEKYVKRGTQLFVEGKLRTREWLDRNTDQKKISVDIVAD
                     NFQVLGRRSEAPQQPQNSHAPASFSIPEDDLPDDSLPF"
     gene            complement(613..1371)
                     /locus_tag="P5293_09245"
     CDS             complement(613..1371)
                     /locus_tag="P5293_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRR50118.1"
                     /translation="MRFAVAGNPVNHSLSPALFRAAYGNSEDSYGLIEAKSVEEVIER
                     FRGEGYDGINVTAPFKEGVLGFVDIRSELDIAIGAANTLLMRNGLIYSFNTDYNGVQE
                     CIKPFAGSGEKVLVIGCGGAGKAAALAVAGLKCQVTVVNRSLEKAQQFAKIHSLAAGR
                     LNDLITLIDRSDIIINTLPSNLELIDRANFTGKTVLEANYKSPQIGRLPFITKSTNYI
                     SGKYWLLYQAVDSFRLFKRTEPDFNSMLLFIENI"
     gene            complement(1390..>1678)
                     /locus_tag="P5293_09250"
     CDS             complement(1390..>1678)
                     /locus_tag="P5293_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRR50119.1"
                     /translation="CSSEECCLWLKREKILIFTSEVQASEWYYNSDFTMPSALVMGSE
                     ANGLSQYWRDNADRRIKIPMKGFVDSLNVSVSAAILCFEAVRQRDSKEDFT"
BASE COUNT          509 a          387 c          303 g          479 t
ORIGIN      
        1 gtcctgcatt tgggccaccc tgaaaccttg caaccacagg ataatctttt gctaaaacat
       61 ccacgatctt acccttacct tcatctcccc attggagccc gagaattaca tcaactttca
      121 tatgttacca gtttttactt aataaaaatt aaaatggcag actgtcatcc gggagatcat
      181 cttcaggaat tgaaaaagaa gcaggggcgt gtgaattttg aggttgctgc ggagcctcac
      241 ttcttcggcc aagaacttga aaattatcag ccacgatatc taccgaaatt ttcttttgat
      301 cggtattacg gtcaagccat tctctagttc tcagttttcc ttcgacaaac agttgtgtgc
      361 cgcgtttcac atatttctca gcgcgttctg caagagacct ccagcacaca atattgtgcc
      421 actcagtctg ttcctgccat tttccgtctc tgtcacgata cttttctgta gtagccagag
      481 taaatttggc aactatattg tcgttgtcaa gatgccttat ttcaggatct ttcccaacat
      541 tgccaatgag cataaccttg tttaatgaca taatgaatcc ccccttaata gtaatgacaa
      601 agatatataa attcagatat tttcaatgaa caaaagcatc gagttaaaat caggctctgt
      661 tctcttaaaa agtctgaatg aatcaacagc ctggtagagc aaccaatatt tgcccgaaat
      721 gtaatttgtt gatttggtaa tgaatggcaa tcttcctatt tgaggagatt tgtagttggc
      781 ctcaaggacg gttttgccag taaaatttgc tcggtcaatc aattcgagat ttgaaggcag
      841 agtatttata attatatccg atctatctat caaagttatc aagtcattca ggcggccggc
      901 ggcaagcgaa tgaattttgg caaattgttg agccttttct aaagaacggt taacaacggt
      961 aacctgacac ttaaggccgg ccaccgccag ggcagcagct ttaccagcac ctccgcatcc
     1021 tatcacaagc accttctcac cactgccagc aaaaggtttg atacactcct gaactccatt
     1081 atagtcagta ttgaaagaat aaatcaatcc atttctcatc aaaagagtat ttgcagcacc
     1141 aatagcaatg tcaagttcac ttcggatgtc cacaaaccca agaacaccct ccttaaaagg
     1201 agctgtaaca ttaattccgt catagccctc acctctgaac cgttcaataa cttcttctac
     1261 agattttgct tcaattagtc cataactatc ttcagaattg ccataggcag ctctgaacag
     1321 ggcagggctg agagagtgat tgacaggatt accggcgaca gcaaaacgca ttataataaa
     1381 aattagaatc tatgtaaaat cttctttgga atccctctgg cggacagctt cgaaacagag
     1441 aattgcagca gagaccgaca catttagaga atcaacaaac cccttcatgg gaatttttat
     1501 tcttcggtct gcattgtctc tccaatattg agaaagacca ttagcctcac ttcccatcac
     1561 caaagccgaa ggcatggtaa aatcggaatt atagtaccac tcagaagcct gtacctcaga
     1621 ggtaaaaatt aaaatttttt ctcttttaag ccaaagacag cattcttcgg aagagcaa
//
LOCUS       DAOTQL010000254         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_59309_length_1661_cov_4.301993, whole genome shotgun sequence.
ACCESSION   DAOTQL010000254 DAOTQL010000000
VERSION     DAOTQL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59309_length_1661_cov_4.301993"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1021
                     /locus_tag="P5293_09255"
     CDS             131..1021
                     /locus_tag="P5293_09255"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRR50120.1"
                     /translation="MIDFKQVTISDKIWIDELLRYSDYRGAEYCFTTLFIWDESYLSR
                     CARYKDFLLLKSGSGEETRYLYPAGRGDRREVIELLMQDADERGNSFSLISLTPQTKA
                     ELEELFPGTFNFKVVRNNFDYIYETEKLISLSGKKLQSKRNHINYFKDFFKGWSYEEL
                     TEDTIDEIIGFNEEWCKEADCGKSQSLCLESAAVSKCLANFGKLSVKGGVLRIDGKIV
                     AYTIGEQLNSDTFIIHIEKAFASIRGAYPMINREFAQREAIGLKYINREDDAGDEGLR
                     KAKNSYHPAFMLEKFNAFIK"
     gene            1179..>1370
                     /locus_tag="P5293_09260"
     CDS             1179..>1370
                     /locus_tag="P5293_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50121.1"
                     /translation="MLSSGEKEEEEAMRFAFIQAEVVLITSTLLTVLSIFRLFMNGGE
                     EGFIFYLLSFFVTIILLFLS"
     assembly_gap    1371..1380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1381..>1661
                     /locus_tag="P5293_09265"
     CDS             <1381..>1661
                     /locus_tag="P5293_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50122.1"
                     /translation="QGFDFVKKRKHLISVIYWFGKPLYALIAKLKEIYGKVAEEVTAQ
                     SIEEITEVDDTMDKEEVEEKLLLKGIVGFQNRSAKDIMTPRISIVSLDK"
BASE COUNT          557 a          239 c          345 g          510 t
ORIGIN      
        1 acacaaaaat gttgttgtaa tggtgcaacc aaaaggaagc cacagatccc gcataggtaa
       61 tatgccggag gagctcagaa atgaggccat aagaacactc aagcaatggc tttctgaata
      121 aataaattat atgatagatt ttaagcaggt tacaatttca gacaagatct ggattgatga
      181 attgctgaga tactccgatt acagaggagc ggagtattgt tttacaactc tctttatatg
      241 ggatgagtct tacttgtcac gttgtgcacg atacaaggat tttcttttgt taaagtcggg
      301 aagtggagaa gaaacccggt atttgtatcc tgcaggaaga ggtgacagac gtgaagtaat
      361 agagttgtta atgcaggatg cagatgagag aggcaactca ttcagtttaa tatctctcac
      421 tccacagaca aaagcagaat tagaagagct ttttcccgga acattcaatt tcaaggttgt
      481 tagaaacaac tttgactata tatatgaaac tgagaaatta atttcattat ctggcaaaaa
      541 attgcagtcc aaaaggaatc atattaatta ttttaaggat ttttttaagg gatggagtta
      601 tgaagagctt actgaagaca caatcgacga gataattggt tttaatgaag aatggtgtaa
      661 ggaagccgat tgtggtaaaa gtcagtcact ctgtcttgaa tctgcagctg tttctaaatg
      721 tttggcaaac tttggcaagt tgtcagttaa aggaggtgtc ctcagaattg acggtaaaat
      781 tgttgcttac acaataggtg agcaacttaa ttcagacaca tttattattc atatagaaaa
      841 ggcttttgct tcaatccgag gtgcctatcc aatgatcaat agagaatttg ctcaaagaga
      901 agcaataggt ttgaaatata tcaaccggga agacgatgct ggagatgaag gtttaagaaa
      961 agccaagaac tcctatcacc cagcatttat gttagaaaaa ttcaacgctt ttatcaaata
     1021 gtaattataa tttttatatt tgtgtccaga accattagct tagtttatta gagttggaac
     1081 ctcccagttg caatctgacc gttgattatt tcttactggg gattgtttta atcctcatat
     1141 tactcgtaaa ttcccttgta aaaggaatag agattgtttt gctttcttca ggagaaaaag
     1201 aagaggaaga ggcaatgcgg tttgcattta ttcaagctga agtagtacta ataacttcaa
     1261 cattgctgac tgttctttcg atattcagac tttttatgaa tggtggcgaa gaaggattta
     1321 tattttattt actctctttt tttgtgacca ttatattact ttttctaagt nnnnnnnnnn
     1381 acaaggtttt gattttgtca aaaaaagaaa acatctgatt tcagttattt actggttcgg
     1441 gaaacctctt tatgcattga ttgcaaaatt aaaggagatt tatggcaaag ttgcagagga
     1501 ggttactgct caatccatag aagaaatcac agaggtagat gacacaatgg ataaggaaga
     1561 agtcgaagaa aaactcttgc ttaaaggaat agttggattt caaaatagaa gtgcaaagga
     1621 tataatgact ccaaggataa gtattgtatc tctggataaa a
//
LOCUS       DAOTQL010000255         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_60403_length_1641_cov_2.696721, whole genome shotgun sequence.
ACCESSION   DAOTQL010000255 DAOTQL010000000
VERSION     DAOTQL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60403_length_1641_cov_2.696721"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="P5293_09270"
     CDS             complement(<1..312)
                     /locus_tag="P5293_09270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRR50123.1"
                     /translation="MEHNPKVSVIIPVYNSADYLKETLLSVISQTLRDTEIIVVNDGS
                     TDKSEEIIESYQKGDSRIKYLFQPNQGQSIARNVGLDNATGDFVYFMDSDDLLEQDAL
                     GM"
     gene            complement(296..1078)
                     /locus_tag="P5293_09275"
     CDS             complement(296..1078)
                     /locus_tag="P5293_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRR50124.1"
                     /translation="MKTGNPYDKNSLHIKKDYKVLEVGPGSYPTKRANVLVEKYLYNN
                     RYRRGDFKIYLHQTVIEADAVELPFNNDEFDYVICSHVIEHVDDPIRFAAEQSRVAKA
                     GYLEAPSIIGEFLAPKKSHKWVSLEIDNKFVMFEKSALPFEFKADFGDLFLNYLPYHS
                     LPYRLQVMTRNNFNAVRYEWKDSIDLIVNPADEYLQSFFVKQWDITMVRQIFPEYSIL
                     KETNSTLKAFCHFIKLKLLRDLGLIKDPVSFETYNKMHGAQS"
     gene            1152..>1641
                     /locus_tag="P5293_09280"
     CDS             1152..>1641
                     /locus_tag="P5293_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRR50125.1"
                     /translation="MESLTIAYCIPSLYSMSGMERTLTVKSNYFAENYNYKIYLILTD
                     GGDKDPAFPLSSKITIINLDINYDKIWGKPFFKQLVGYLNKQIKFRKRLTQKLHEIKP
                     DITISMLRRDINFITKIHDGSKKVGELHFNKNNYRDLNNLPNLPLRNFFAKLWMHQLV
                     SNL"
BASE COUNT          569 a          289 c          238 g          545 t
ORIGIN      
        1 catccccaat gcatcttgct ccaaaaggtc atcactatcc ataaaataga caaaatctcc
       61 tgttgcatta tcgagtccta cattccttgc aatagactgc ccttgatttg gttgaaacaa
      121 atattttatt cttgagtcac ctttctgata agattcaatt atttcttcac tcttatcagt
      181 agagccatca ttgactacaa taatttcagt atctctaaga gtctggctaa taaccgacag
      241 gagagtctct ttcaaataat ctgcagaatt gtaaacagga attatgacac taactttagg
      301 attgtgctcc atgcatttta ttataagttt caaatgatac tggatcctta ataaggccta
      361 aatctcttag taacttcagt tttatgaaat gacaaaaagc ctttaatgtg ctattggtct
      421 ccttcaatat agaatattca ggaaagattt gtctaaccat agtgatatcc cattgtttaa
      481 caaaaaagga ttgcaaatat tcatcagcag gattaacaat taaatcaata gaatctttcc
      541 actcatacct tacagcatta aaattatttc ttgtcattac ttgtagtctg tatggcaaag
      601 agtgatatgg tagatagttc aaaaaaagat ctccaaaatc agccttaaat tcaaatggta
      661 aagctgattt ttcaaacatt acaaatttat tgtctatttc aagtgaaacc catttgtgag
      721 atttttttgg agccaaaaat tctccaataa tactgggagc ctccagatat cctgcttttg
      781 cgactctgct ctgttctgct gcaaatctga tcggatcgtc aacatgctca attacatggc
      841 tacaaataac ataatcaaat tcgtcattgt taaaaggcag ttcaacagca tctgcctcta
      901 taacagtttg gtgtaaataa attttgaagt ctccccttct gtaacgatta ttatatagat
      961 acttttcaac cagcacatta gctctttttg tagggtaact ccctggacca acttcaagaa
     1021 ccttataatc tttttttata tgaagagaat tcttatcgta tggattgcct gttttcataa
     1081 aattatatac tttgtaaaat taagaaaaat cctaaatttg attaaaaatc attcaacaaa
     1141 agggcttgat tatggaatca ctcacaattg catattgtat accatcactc tattcaatga
     1201 gtggtatgga gagaactcta actgtcaaga gcaattactt tgcagaaaac tataattata
     1261 aaatctattt gatcctgaca gatggtgggg ataaagatcc ggctttccct ctttcttcaa
     1321 aaataacaat aattaatctt gatattaatt atgataaaat ctggggaaaa ccctttttta
     1381 aacaattagt cggttattta aacaagcaga tcaaattcag aaagagactt acacaaaagc
     1441 ttcatgagat aaaacccgat atcacaatat caatgttgag aagagatatt aattttataa
     1501 caaagataca tgacggaagt aaaaaagttg gagaacttca ttttaataaa aacaattata
     1561 gagatttgaa caatttgccc aatctgcctc taaggaattt ttttgcgaaa ttatggatgc
     1621 atcaacttgt ttctaatctg a
//
LOCUS       DAOTQL010000256         1639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_60492_length_1639_cov_5.215278, whole genome shotgun sequence.
ACCESSION   DAOTQL010000256 DAOTQL010000000
VERSION     DAOTQL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60492_length_1639_cov_5.215278"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /gene="hisC"
                     /locus_tag="P5293_09285"
     CDS             complement(<1..1110)
                     /gene="hisC"
                     /locus_tag="P5293_09285"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRR50126.1"
                     /translation="MMELNKLVRKNIRELNSYSTARDEEGERISIYLDANENPFENGV
                     NRYPDPKQRDLKKIISSIYNVPVKNIFAGNGSDEAIDLLYRVFCEPKSDNVLSISPTY
                     GMYKVAAQINDVEFREILLEDDFSINPEKMITAADNNTKLIFVCSPNNPTGNIIDENS
                     IVQIANNFDGIVVIDEAYCEFSRKPGFIGKIESHPNIVVLRTLSKAWGMAGLRIGFAI
                     ADERIVSFLSSVKYPYNIGSDTLSLAVKYLNRSPASKIDKIISERERVSAHLENLLDV
                     EKVFPSDANFILVKFKDSSSIYKKLAENGISVRDRSNQPKCDNCLRLTIGLSEENNKL
                     LKVLAGENLNQDINETRRAFIERRTKETYVSLKMEF"
     gene            complement(1094..>1639)
                     /locus_tag="P5293_09290"
     CDS             complement(1094..>1639)
                     /locus_tag="P5293_09290"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRR50127.1"
                     /translation="DLLSQAEHGNDSQLSFLTTDKYLAEEVNRLLMLQLSELPRRSIA
                     EISISNGYSIVCSSVDEMVDFANLYGAEHLIISMREPWNIADRIENAGSVFLGNYSPE
                     SAGDYASGTNHTLPTAGWTCSTGGISVDSFMHKITLQQITSEGLAALGGTIEKMAEAE
                     GLTAHKNAVSIRLGRINDGVK"
BASE COUNT          455 a          349 c          274 g          561 t
ORIGIN      
        1 gaattccatc ttaagagaga cataggtctc ttttgttctt ctctctataa atgctcttct
       61 tgtttcatta atatcttggt tcagattctc tcctgcaagc actttcaaaa gtttattatt
      121 ttcctccgaa aggccgattg tcaatctaag acaattatca cattttggct ggttggaacg
      181 atctctcaca gatataccgt tttcggcaag ttttttatat atagagctac tgtctttgaa
      241 tttgaccaaa atgaaatttg catcactcgg aaaaactttt tcaacatcta ataagttttc
      301 caaatgagcc gaaactctct cccgttctga tattatctta tctatctttg atgcaggact
      361 cctgttgaga tacttcacag ccagagaaag cgtgtctgaa cctatattgt atggatattt
      421 cacagacgag agaaaagaca caattctttc atctgcaatt gcaaatccta ttcgtaagcc
      481 ggccattccc catgctttgg ataatgtacg caggacaaca atatttggat gactctctat
      541 ttttccaata aaccctggct tccgggaaaa ttcacaatag gcttcgtcta ttacaactat
      601 tccgtcaaaa ttattagcaa tctgtacaat tgaattttca tctattatat tacctgtcgg
      661 gttgttggga gaacagacaa aaatcaactt agtgttgttg tctgctgcag ttatcatttt
      721 ttcaggattt atagagaaat catcttcaag aagtatctct ctaaattcca catcgtttat
      781 ttgagcagct actttgtaca ttccataagt tggagaaata gatagaacgt tgtccgactt
      841 tggctcgcaa aaaactctgt acaacaagtc tatggcctca tcacttccat taccggcaaa
      901 gatattcttc accggcacat tgtatattga agagataatt ttctttaaat ctcgttgttt
      961 aggatcaggg tatctgttta ctccattctc gaaaggattt tcgttggcat cgagatatat
     1021 cgagatcctc tcgccttctt catcccttgc tgtagaataa ctattgagct ctcttatgtt
     1081 ttttctgact aatttattta actccatcat ttatcctccc caatcttatt gaaactgcat
     1141 ttttatgtgc tgtaagacct tctgcctcag ccattttttc tattgtacca cctaaagcag
     1201 ccagaccttc gcttgtaatt tgttgaagag ttattttgtg cataaaacta tccacagaga
     1261 ttcctcccgt tgagcatgtc caaccggccg taggtagagt atgatttgtg cctgaagcat
     1321 aatctcctgc actctccgga gaatagtttc caagaaaaac acttccggcg ttttcaattc
     1381 ggtcagcaat attccatggt tctctcatgg aaataatgag gtgttctgct ccataaaggt
     1441 ttgcaaaatc caccatttca tctacactac tgcatactat cgaatatccg ttgcttattg
     1501 aaatttcggc aattgacctg cgagggagtt cagataactg aagcattaat aacctgttaa
     1561 cctcttctgc cagatattta tcagttgtga gaaaagatag ttgcgaatcg tttccatgct
     1621 ctgcctgtga aagaagatc
//
LOCUS       DAOTQL010000257         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_60846_length_1633_cov_2.987326, whole genome shotgun sequence.
ACCESSION   DAOTQL010000257 DAOTQL010000000
VERSION     DAOTQL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60846_length_1633_cov_2.987326"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="P5293_09295"
     CDS             complement(<1..396)
                     /locus_tag="P5293_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRR50128.1"
                     /translation="MNYGNLIKISFRALMNNRLRGFLTMLGVIIGVASVIAMLAIGQG
                     SKASIKNQISEMGSNLIMIQPGNGQFGGVRLSASSMQTLKLADYQRVANEAQYISRCS
                     PIVSSSGQIIYGSNNAPSTITGVNTEYLEI"
     gene            complement(393..1094)
                     /locus_tag="P5293_09300"
     CDS             complement(393..1094)
                     /locus_tag="P5293_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR50129.1"
                     /translation="MSEIIKTEKLSRDFVIGDETIHALREVSFTIKEGEFVTIMGASG
                     SGKSTLLNLLGCLDTPTSGEYYLDGISVKTINKNERATLRNRKIGFVFQSYNLLPKTT
                     ALENVELPLFYNPSVSQKEREKRAMAALKSVGLADRMNHKSNQMSGGQMQRVAIARAL
                     VNNPVLLLADEATGNLDSKTSAEIITLFQSLHREGRTIVFVTHNPELSQYSSRTIVIK
                     DGIIIEDVANKNYRK"
     gene            complement(1100..>1633)
                     /locus_tag="P5293_09305"
     CDS             complement(1100..>1633)
                     /locus_tag="P5293_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRR50130.1"
                     /translation="VDEADIGEVEEGQKVTFTVDAFPDDIFNGNVTQVRLQPTTTSNV
                     VTYEEVVDAFNPNYKLKPGLTANITIITMEKEDILMVPVKALRFSPESEQNKETKVSD
                     NSTKRIWIKKSDGALLPVDVITGVSDGIHIEVTGNIKAGDLVVTGLSTSPVKTVSKKS
                     DTGESPFMPARPGQKKK"
BASE COUNT          415 a          345 c          288 g          585 t
ORIGIN      
        1 tatttcaaga tactccgtat ttactcccgt tattgtgctt ggagcattat ttgagccgta
       61 aataatttgt ccgctactac ttacgatagg tgaacaacga gagatatatt gtgcttcatt
      121 agccacccgt tgataatctg caagtttaag cgtttgcatg ctgctggcac tcagtcttac
      181 ccctccgaat tgcccgtttc cgggttggat cataattaaa ttggagccca tttctgaaat
      241 ttgatttttg atactggcct tggagccttg gccgatcgca agcattgcaa tgacagaagc
      301 aacccctatt atgacaccaa gcatagtgag aaatcctctt aacctgttat tcatcagggc
      361 tctgaaggat atttttatta aattgccgta attcattttc tataattttt attagcgaca
      421 tcttcgataa ttattccgtc ttttattact atagttctac tgctgtattg agataattcg
      481 ggattgtgtg taacaaatac aatcgttctt ccctctctgt gaagtgattg aaagagagtg
      541 ataatttctg ctgatgtttt agagtcaaga ttaccagtag cctcatcggc cagtagcagt
      601 acaggattat tgacgagtgc tcttgcgatt gccactctct gcatttgtcc tcctgacatc
      661 tgattggatt tatgattcat tctgtctgca agacctacag acttaagagc tgccatagct
      721 cttttttctc tctctttttg tgaaactgaa gggttgtaaa aaagaggaag ttctacattt
      781 tccaatgcag tggtctttgg aagaagatta tatgactgaa atacaaatcc gatttttcga
      841 tttctcaaag tagccctttc attcttattt atggttttaa cagatatccc atccaggtaa
      901 tattctccag atgttggagt atcaagacat ccaagcagat tgagcagtgt ggatttgccc
      961 gaaccgctcg cccccatgat tgttacaaac tctccttctt taattgtgaa actgacctcc
     1021 ctcaaagcat gtattgtttc atctccaatt acgaagtctc ttgacagctt ttctgttttt
     1081 ataatttcac tcataatttt tattttttct tttgacctgg acgcgccggc ataaaagggc
     1141 tttctcctgt atcacttttc tttgacacag tcttcacagg tgatgtcgac aatcctgtaa
     1201 ctacaagatc acctgcctta atgttgcctg ttacttctat atgaatacca tcagatacac
     1261 cagtaattac atctacaggc aagagtgccc cgtcactttt tttaatccat attcttttcg
     1321 ttgagttatc tgagaccttt gtctctttat tttgttcaga ctccggagag aatcttaaag
     1381 cctttacagg aaccattaga atatcttctt tttccatggt gataatagta atatttgctg
     1441 tcaatccggg tttaagttta tagttcggat tgaaagcatc cactacctct tcatatgtta
     1501 ccacatttga tgttgtagtt ggttgaagcc ttacttgtgt tacgtttccg ttaaatatat
     1561 cgtccgggaa tgcatctaca gtaaatgtga ctttttgtcc ttcttccact tctccaatat
     1621 cagcttcgtc cac
//
LOCUS       DAOTQL010000258         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_61780_length_1616_cov_4.300448, whole genome shotgun sequence.
ACCESSION   DAOTQL010000258 DAOTQL010000000
VERSION     DAOTQL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61780_length_1616_cov_4.300448"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1402
                     /locus_tag="P5293_09310"
     CDS             206..1402
                     /locus_tag="P5293_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRR50131.1"
                     /translation="MLINCGMSPHLADWLDEIISITLLFLIVYVADLICRFIVNKVVG
                     HIAKMTKSRWDDIFLEYKVFRYLTHAIPGLIFFLCTPIAIKSVIWVKLFQKAALIYIT
                     VVIIRAIVAATNAMTRIYAESESYAKKPVKIIFQIVAVIAYFIGGISILSIIINASLA
                     TLFAGLGAFMAVLMLVFKDSILGFVAGWQLSSNDMLRIGDWITAPKHGADGTVEEVSL
                     YSVKVRNFDNTIVTIPPYSLVSDSFQNWRGMEESEGRRIKRYISIDMTSVKFCTPEMI
                     EKFRKIHYLKDYIENTEQIITEYNEENKIDNSVLVNGRRQTNIGVFRAYLRSYINNHP
                     DINHNLTCIVRQLQPSEKGVPIEIYCFTKEKGWINYENVQSDIFDHVIAIVDQFDLKI
                     YQLKSV"
     gene            complement(1389..>1616)
                     /locus_tag="P5293_09315"
     CDS             complement(1389..>1616)
                     /locus_tag="P5293_09315"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRR50132.1"
                     /translation="FNNKIMTELSEENWIWRSIYLRLRREGFNNVNYIKGAELVGEDN
                     EGTVDGVHMSDLGFYRYANILSKYLSSSKHF"
BASE COUNT          525 a          308 c          299 g          484 t
ORIGIN      
        1 ctcttgaatg ggtgaaagag aacaaatttt ggccatatgt tgccagagta gataatggat
       61 atggtgatcg caacttaaag tgcggttgcg atgcatggcc atccttactg taattattac
      121 tatacaatta aatgctatct tgatcgagga gcaaataaca accagagcta cagccctttt
      181 cggagtagta gatgccataa acaaaatgct tataaattgt ggcatgtctc ctcatcttgc
      241 agactggcta gatgaaatta tcagtataac tcttctattt ctaattgtat atgttgccga
      301 cctcatttgc aggtttatag taaataaagt tgtaggtcat attgcaaaaa tgacaaagtc
      361 cagatgggat gatatatttc tggaatataa agtatttcgt tatctaacac acgctatccc
      421 aggacttata ttttttcttt gcactccaat agccatcaaa tctgtaatct gggtaaaatt
      481 atttcagaaa gcagctctca tatacataac tgtggttatt ataagagcta ttgtggctgc
      541 cacaaatgcc atgactcgca tttatgccga atctgagagc tatgcaaaaa aacccgtcaa
      601 gattattttc cagattgttg ctgtaatcgc ttactttatt ggaggcatct caattctcag
      661 catcattata aatgcctccc ttgcaaccct ctttgctgga ctcggggctt tcatggctgt
      721 cctcatgctt gttttcaagg acagtattct cgggtttgtg gccggatggc aactttcctc
      781 aaatgacatg ttgcgcatcg gagactggat tactgctcca aaacacggag cggatggaac
      841 tgtagaagaa gtaagtctat actccgtaaa agttagaaat ttcgataata ctatcgttac
      901 gattccgccc tactctctgg tcagcgattc tttccagaac tggcggggga tggaagagtc
      961 agaaggcaga cgaatcaaac gttacataag tattgacatg acaagtgtca aattctgtac
     1021 acctgaaatg atagaaaagt ttagaaaaat acactattta aaggactata ttgaaaatac
     1081 agaacaaata ataactgagt ataacgaaga gaataaaata gacaacagtg tgctggtcaa
     1141 tggaagaagg cagacaaaca taggggtttt cagagcttac cttcggtcat atatcaataa
     1201 ccatccggat attaatcaca atcttacctg tatagtgaga cagcttcaac caagtgaaaa
     1261 aggagtacct atcgaaattt actgtttcac caaagaaaaa ggatggataa attacgaaaa
     1321 tgttcagtca gatatatttg atcacgtaat tgcaatagtc gatcagtttg atctcaaaat
     1381 ctaccaactc aaaagtgttt agaacttgaa agatatttag aaagtatatt tgcatatcgg
     1441 taaaacccca aatcactcat atgcactcca tctacagtgc cctcattgtc ttccccgaca
     1501 agttccgctc ctttaatgta attaacattg ttaaatcctt ctcttctaag tctgagataa
     1561 atagacctcc atatccaatt ctcttcagaa agttcagtca tgattttatt gttaaa
//
LOCUS       DAOTQL010000259         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_64607_length_1568_cov_2.694646, whole genome shotgun sequence.
ACCESSION   DAOTQL010000259 DAOTQL010000000
VERSION     DAOTQL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64607_length_1568_cov_2.694646"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /gene="argS"
                     /locus_tag="P5293_09320"
     CDS             <1..749
                     /gene="argS"
                     /locus_tag="P5293_09320"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRR50133.1"
                     /translation="NLDEHIYVVGNEQNYHFQVLKLILKKLGFSWADSIYHLSYGMVE
                     LPEGKMKSREGTVVDADDLIENMVNTAKETSLELGKLENMSKEEQENLFEILGLGALK
                     YFIIKVDPKKTMLFDPKESIDFNGNTGPFIQYTHARIKSILRKAIEYKFVPGLNDVDF
                     NEKEIEIIKLLTLFPDRIKQGGEELSPALIANYCYDLAKEFNQYYHEVSVLKEDDSKV
                     RDQRLALISKIATVLSKGMKILGITLPERM"
     gene            751..>1568
                     /locus_tag="P5293_09325"
     CDS             751..>1568
                     /locus_tag="P5293_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRR50134.1"
                     /translation="MISVSENFIPTSKKEVEALGWDYIDVIIFSGDAFVDHPSFGTAV
                     IARVLQKEGYKVAVVPQPNWRDDLRDFRKLGKPRLFFGVNSGVMDSMVNHYTANKRLR
                     SNDAYTPGGKSGQRPDYAVTVYSQILKKLYPDVPLVIGGIEASLRRLTHYDYWKDALQ
                     PSILFDSGADILVYGMGEKSIIEIAGKLAAKASVEQLHNIPQIGFINDFEDLSLSDNK
                     LLLNSYEECLENKKTFVNNFNLIEKESISINSRHIIEPAKGRFVHINPPYGLLS"
BASE COUNT          517 a          243 c          324 g          484 t
ORIGIN      
        1 ataatcttga tgaacatatt tatgtagtgg gaaacgagca gaattatcat ttccaggtgc
       61 ttaaattgat tcttaagaag cttggtttca gttgggctga ttcaatttat catttgtcat
      121 atggcatggt tgaactcccg gaaggaaaaa tgaaatctag ggaaggaaca gttgttgatg
      181 cagatgatct tatagagaat atggttaata cggcaaaaga gacctctctt gaattgggca
      241 aactggaaaa catgagtaaa gaggaacagg aaaacctttt tgaaattttg ggcttgggtg
      301 cgctcaagta ttttataatt aaagttgacc ctaagaaaac aatgttgttt gatccaaaag
      361 aatcaattga ttttaatgga aacactggac cgtttattca atatacccat gcgagaatca
      421 aatcaatact aaggaaggca atagagtata aatttgttcc aggtctcaat gatgtggatt
      481 ttaatgaaaa agagatagaa attataaaac tgctgactct ttttccggac aggataaagc
      541 aaggaggtga agagctttct cctgcactta ttgccaatta ttgttatgat ttggctaaag
      601 agttcaacca atactatcac gaagtatcag ttttaaaaga ggatgattct aaggttagag
      661 atcagagact tgctctcata tcaaaaattg caacagtgct ttcaaaaggg atgaaaatcc
      721 ttggcatcac tcttcccgaa agaatgtgaa atgatttcag tttcagaaaa ttttattcct
      781 acttcaaaaa aagaagttga agctctcggt tgggattaca ttgatgttat aattttttcg
      841 ggtgatgcat ttgtcgatca cccatctttt ggcacagctg ttattgcaag agtgcttcag
      901 aaagaaggat acaaagttgc agttgtccct cagccaaatt ggagggatga tttacgtgat
      961 tttcgcaagt tggggaagcc cagacttttt tttggagtta attctggtgt aatggattcc
     1021 atggtaaacc attatactgc caataagcgg ctaagaagta acgatgcgta tacgcctggc
     1081 ggaaaatcag gacaaagacc agactatgca gtgacagtgt attcacaaat acttaaaaaa
     1141 ctttatccgg atgtaccatt agttattggg ggcatagagg cctcactgcg acgtcttaca
     1201 cattatgatt attggaaaga tgctttgcag ccgtccatcc tttttgattc cggtgctgat
     1261 attcttgtat atggaatggg tgaaaaatct attattgaaa ttgctggcaa actggcggca
     1321 aaagcatctg tagagcaatt acacaacata ccacagattg gttttataaa tgattttgaa
     1381 gatctatctt tatctgacaa taaattatta ttaaattcct atgaggagtg cctggaaaat
     1441 aaaaaaactt ttgttaataa ttttaatttg attgagaaag aatcaatttc aattaattca
     1501 aggcacatta ttgagccagc aaagggaaga tttgtccata tcaatcctcc ttatggactt
     1561 ttgtccca
//
LOCUS       DAOTQL010000260         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_65067_length_1561_cov_2.179283, whole genome shotgun sequence.
ACCESSION   DAOTQL010000260 DAOTQL010000000
VERSION     DAOTQL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65067_length_1561_cov_2.179283"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="P5293_09330"
     CDS             complement(<1..933)
                     /locus_tag="P5293_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50135.1"
                     /translation="MKRRDFIRNTTLGGASVAITSRVNIPFVQNRPSTLTINSTPLFE
                     LSPWLYMQFMEPLGVTDSSVEAAWDHQNDRWREDVVQVTKDLSPGMIRWGGIFSSYYR
                     WKEGVGPRNKRKPMLNINWNGIESNQVGTAEFVDFCRQVRADPLMVVNFESEGFAEYA
                     ATKKGELRSADSKEAGEWVDYCNNPSNKLRIKHGFKDPLTIKTWQLGNETSYGRGKFN
                     VEQASSKTIEFAKAMRKVDPSIKLIGWGDSGWAKRMIDIAGEYIDFIAFHHMFNPGQN
                     IKNSPLRDNEYRKDPAGTWEILMDAYKIHENKIRE"
     gene            complement(1149..>1561)
                     /locus_tag="P5293_09335"
     CDS             complement(1149..>1561)
                     /locus_tag="P5293_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50136.1"
                     /translation="SFTEPSSDWYKRCNLGKWNSGLGPFGAKQMLTPEVNSIWSTRSL
                     YLQKDFNLPSIPKKAAIVIYNVGITDIYINGEFAIRINNLRRWDTEVKVSEVTLPPNA
                     ASLLRKGTNCISVKFEFVQTDEPFNYFDIGLKIY"
BASE COUNT          427 a          328 c          258 g          548 t
ORIGIN      
        1 ttctcttatt ttattctcat gaattttata ggcgtccata agtatttccc atgtgccggc
       61 cggatccttc cgatactcat tatcacgaag aggtgagttt ttaatattct ggccaggatt
      121 gaacatatgg tggaaagcaa taaaatcaat gtactctcct gctatatcaa tcatcctttt
      181 tgcccatcca ctgtctcccc atccaattaa cttgatagaa ggatcgactt ttctcatcgc
      241 ttttgcaaat tcaatagttt tactggaggc ttgttctaca ttaaatttac ctctgccata
      301 agatgtttcg ttcccaagtt gccaggtttt tattgtcaga ggatctttaa agccatgctt
      361 tattctgagt ttatttgaag gattattgca ataatcaacc cattcacctg cttcttttga
      421 atctgcagaa cggagttccc ccttttttgt agcagcatac tccgcaaatc cttccgattc
      481 aaaatttact accataagag ggtcggctct cacctgtctg cagaaatcaa caaattctgc
      541 agtacctacc tgatttgatt caattccgtt ccagttgata ttcagcattg gctttctctt
      601 atttcttggg cctaccccct ctttccagcg ataatatgaa gaaaaaatcc ctccccaccg
      661 gatcatccct ggagacaggt cttttgtcac ctgaacaaca tcctccctcc atctatcatt
      721 ctgatgatcc catgctgctt ccaccgaaga gtcagttact cccagcggct ccataaactg
      781 catatacaac catggagaca attcaaacag gggagtggaa ttaattgtca atgttgatgg
      841 cctgttttga acaaaaggga tgtttactct tgatgtaatt gcaacagagg ctcctcctaa
      901 tgttgtgttc ctgataaaat ctcgtctttt cacatttatg attttcttaa taatgttatg
      961 taaagcctct atttttaagt gagtatattc ataattgttt gtttcaccat tgtaggaaag
     1021 agatttctta ttaaaacatt tgtttttaat accagattta atatttcttt atgccaatgt
     1081 caataaattt aacacaggca gattggtaag tctatgtaag tccggtttca aagttctctt
     1141 ataatttatt agtatatttt taaaccaata tcaaaataat tgaagggttc atctgtctga
     1201 acaaattcaa attttacgga gatacaattt gtacctttcc tgagaagact ggctgcattt
     1261 gggggcaatg ttacttcgct aaccttaact tcggtatccc atcttctcag gttatttatc
     1321 cggatagcaa attccccgtt tatgtatata tcggttatac cgacattgta tataacaatc
     1381 gcagctttct taggtataga agggagatta aaatcttttt gcagataaag gcttctagtg
     1441 ctccatatag aatttacctc cggtgtaagc atttgttttg ctccaaatgg accaagtccg
     1501 ctattccatt ttcctaagtt gcatcttttg taccagtcag atgaaggttc tgtaaatgaa
     1561 t
//
LOCUS       DAOTQL010000261         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_66847_length_1533_cov_3.072395, whole genome shotgun sequence.
ACCESSION   DAOTQL010000261 DAOTQL010000000
VERSION     DAOTQL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66847_length_1533_cov_3.072395"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="P5293_09340"
     CDS             complement(<1..187)
                     /locus_tag="P5293_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRR50137.1"
                     /translation="MKKLKIAIQKTGRLSENSHKLLEECGIKFSNGVNVLKTSAVNFP
                     VEVLFLRDDDIPQYVEQK"
     gene            430..>1533
                     /gene="rodA"
                     /locus_tag="P5293_09345"
     CDS             430..>1533
                     /gene="rodA"
                     /locus_tag="P5293_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRR50138.1"
                     /translation="MDQNLSKVENSNLALFLKRFDFLHFFVIISLSLSGVLFIYGIGQ
                     QVGGRIQDYWKMQIIWLAVGFSLWIYLSFFFDLKKLRLLAPIIFVISVLFLILVLFQG
                     QRINMAKRWINIMGFRFQPSELAKTALVIMLAWILTLRDFNVNKLYHLLLCLIIAAIP
                     FILICVEPDLGTAVVIIPLTFSMLFVAGLNKKWIFSGIIAMLILLPSAYPFLKEYQKE
                     RIRVFLDPNRDIRNRGWNSLQAELAVGSGGLYGKGFMQGTQHSLGFLPQTVSNTDFIF
                     SVIGEEMGFIGSLGLVILYLSLCFSLLRTSMSADPFGKYIAVGVCILIFIHSFINIGM
                     TIRLLPVTGLPLPLISYGGTFTINTSILLGLANS"
BASE COUNT          452 a          246 c          262 g          573 t
ORIGIN      
        1 ctttttgttc aacgtactga ggaatgtcat cgtccctcag gaataaaact tcaaccggaa
       61 aattaacagc tgaggtcttt aagacattca ctccgtttga gaatttaatt ccgcactcct
      121 caagcagttt atgtgagttt tcacttaatc ttcctgtttt ctggatcgca atcttaagtt
      181 tcttcattct tataactagt tatttaggtt gatttaaagg cataaaaaaa cccgcctgtt
      241 ctctcagacg ggtctttatg atattttgat ttaactgaca tatcaatttc ctctcgcctg
      301 agagcaagtg tgaaaatgat gatgatgatg taattttatc tgactcatta atacgaaaat
      361 gacatgcaaa tatacaaaat catgaataat aattacaaaa atatcataaa aagttaagta
      421 gtttataaca tggatcagaa tttaagtaaa gtggaaaact ctaatttagc tctttttctt
      481 aaaagatttg attttttgca cttttttgtg attatttctc tttctttatc aggggtgctt
      541 tttatctacg gaataggtca acaggttggt ggcagaatac aggattattg gaaaatgcaa
      601 ataatttggc ttgctgttgg cttttcttta tggatatatc tttcattttt ttttgatctc
      661 aaaaagttaa gattattagc cccaattata tttgttatat cagttttgtt tttgatttta
      721 gttctttttc aaggacaacg tataaacatg gcaaaacgct ggataaatat catgggattt
      781 agatttcaac cttctgaatt ggctaaaact gctctggtaa taatgctggc atggatttta
      841 actttgcgag attttaatgt gaacaaatta taccatcttc tactttgtct aattattgct
      901 gctattccat ttattttaat atgtgttgaa cccgatctcg gaactgcagt agtaataatt
      961 cctttgacat tttctatgct atttgtggct ggccttaata aaaaatggat attttctgga
     1021 atcattgcta tgctcatttt attgccttca gcatacccat ttcttaaaga atatcagaag
     1081 gagagaataa gagtatttct tgacccaaac agggacatca gaaatagagg ttggaatagt
     1141 ttacaagctg aactggcagt aggtagtgga ggcctctatg gaaaaggatt tatgcaggga
     1201 acccaacatt ctcttggttt tcttcctcaa actgtttcaa atacagactt tattttttca
     1261 gtaataggcg aggaaatggg atttattggc tcacttggat tagttatttt atacctttct
     1321 ctctgtttct ctctcctaag aacttcgatg tcggcagatc catttggaaa atatattgca
     1381 gtaggtgtat gtatccttat ttttattcat agttttataa acattggaat gacaattcgt
     1441 cttttgcctg ttactggatt acctttaccg cttataagct atggaggtac atttacaatc
     1501 aatacatcaa ttttactcgg attggcaaac tct
//
LOCUS       DAOTQL010000262         1519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_67844_length_1519_cov_2.740437, whole genome shotgun sequence.
ACCESSION   DAOTQL010000262 DAOTQL010000000
VERSION     DAOTQL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67844_length_1519_cov_2.740437"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="P5293_09350"
     CDS             complement(<1..350)
                     /locus_tag="P5293_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HRR50139.1"
                     /translation="MILQFTKFLYRIKYWLIFLPMVAALVAIYLTKNIIRTYEVNMTI
                     YTGIASGFTIESGVESGRIDWASVNNGMDNLISIIKSKSTLYEVSLRLYAQNMIYGDS
                     TKDNNYILARNYRE"
     gene            complement(356..1102)
                     /locus_tag="P5293_09355"
     CDS             complement(356..1102)
                     /locus_tag="P5293_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRR50140.1"
                     /translation="MKRRATILLIIGLLPTVAWGQKEPIVIVKSDSSDLLLDVILPPI
                     EILFENAKNGPSWEFYNLKKDELNRKYVTEKRSWYQFVDLFGTYQYGVVGMNSYTDLG
                     TNYPIVYQNSGGEQLWYNAGVSVRFPLDKVIDRKNRIKTQKSVVDQAEKEQEMWFEDR
                     KMKIIDQYTKSEEMISNLKTALEQFSLSQAEYDMAEKDYIMGTITISELGIAKGKQVQ
                     SIQQLSKIKSELKNSILQLEILSCTKILNK"
     gene            complement(1107..>1519)
                     /locus_tag="P5293_09360"
     CDS             complement(1107..>1519)
                     /locus_tag="P5293_09360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRR50141.1"
                     /translation="PESVYLREKKIRQKNNFVKYVNDPRITKVGRFLRKFSLDELPQL
                     INILKGDMSFVGNRPLPLYEAEQLTTDEYIDRFFAPAGLTGIWQVEKRGVSGSLSPEE
                     RKQLDIYYAHNYNFWMDIKILFKTLTAFIQKENV"
BASE COUNT          451 a          302 c          221 g          545 t
ORIGIN      
        1 aattctctgt aatttcttgc caaaatatag ttgttatctt ttgtagaatc tccgtatatc
       61 atattttgtg catataatcg caacgatacc tcataaagag tacttttgga tttaattatg
      121 cttatgagat tgtccatacc gttgttgacg cttgcccagt ctatacgtcc tgattccact
      181 cctgactcaa tagtaaatcc agatgcaatc cctgtataaa tagtcatatt gacctcatag
      241 gtgcgaatta tgttctttgt taagtaaatt gcaacaagtg cagcaaccat cggcaaaaaa
      301 ataagccagt atttaattcg atataggaat ttagtgaatt gcaaaatcat ctttattact
      361 tattaagtat ctttgtgcaa gaaaggattt caagctgaag gattgagttt ttaagttctg
      421 actttatttt tgataattgc tgaattgatt ggacctgttt gccttttgct attccaagct
      481 ctgaaatagt aatagtcccc ataatatagt ccttttccgc catgtcatat tctgcttgag
      541 aaagagaaaa ctgttccaaa gctgttttaa gattacttat catctcttct gatttggtgt
      601 attgatctat tattttcatc tttctgtcct caaaccacat ctcctgctct ttttcggcct
      661 gatcaaccac actcttttga gtttttattc tattttttct gtcaataacc ttgtccagcg
      721 gaaatctgac tgaaactcct gcattgtacc aaagttgctc tccaccactg ttttgataaa
      781 caataggata atttgtcccc agatcagtat aagagttcat cccaaccact ccgtactgat
      841 atgtgccaaa taaatcgaca aactgatacc aactcctctt ctctgtaaca tattttctat
      901 taagttcatc cttcttcaaa ttgtaaaatt cccaagaggg accattctta gcattttcaa
      961 acagaatttc aataggaggt aaaataacat ccagcaataa atcactactg tcagatttaa
     1021 caatcacaat aggttctttc tgtccccatg caactgttgg gagtaaccca ataattaaca
     1081 atattgtagc tcttctcttc ataaaatcat acgttttctt tttgaataaa agcagttaga
     1141 gttttaaaaa gaatttttat atccatccaa aagttgtaat tatgtgcata atatatatct
     1201 aattgcttac gttcttccgg agataggctg cctgaaacac ctctcttttc tacctgccat
     1261 attcctgtaa gtcccgcagg agcaaaaaat ctgtcaatat actcatctgt tgtgagttgc
     1321 tctgcttcat atagaggcag tggtctgtta cctacaaaag acatgtcgcc tttgagtatg
     1381 ttaatcaatt gaggtaattc atccaaactg aacttcctta aaaatcttcc tacctttgtt
     1441 atacgtggat cattgacata tttaacaaaa ttatttttct gacgtatttt tttctctctt
     1501 aaatatacag attcgggaa
//
LOCUS       DAOTQL010000263         1506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_68704_length_1506_cov_3.966230, whole genome shotgun sequence.
ACCESSION   DAOTQL010000263 DAOTQL010000000
VERSION     DAOTQL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68704_length_1506_cov_3.966230"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="P5293_09365"
     CDS             complement(<1..1421)
                     /locus_tag="P5293_09365"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRR50142.1"
                     /translation="MEVDYNRPQVYEIGGLTVSGIKYLNPDQILSLTGIQIGDKIEIP
                     GEELSSIIKRIWQQRWFSEIAFYIDSVSNGKVYLNLYLQERPRISMLEFEGVKSSERT
                     ELIDKLKIKRGGELSDYLEKSACDIIKKYYVDKGFLQTEVKVLKVQDTTINNAIRVTF
                     VVDRKQKVKIQDINFNGNENIKDSKLAGSMKKTRAKSRVLNVLSSKKFNEKEYENDKK
                     NIIKVYNEKGFRDAKIVKDSIYYVRNGRLGIDITIDEGRQYYFRNITWTGNSQYSSDF
                     LNSILRIKKGDIYDMVSMEERLTRDEKGNVSKLYTDNGYLFFNVTPVEVRIENDSVDV
                     EMRIYEGKPAIFNRIMISGNTVTNEKVVRRELYTRPGYLYSQSNIERSLRELANMGHF
                     EPEKLQSADAINIVPNQHDNTVDITYNVVEKSNSQFEIAGGWGGNTFVGTLGVSFNNF
                     SIKRIFDKRAWRPVPLGDGQQLA"
BASE COUNT          421 a          335 c          221 g          529 t
ORIGIN      
        1 aaggcaagct gctgtccgtc tcccaatgga acaggtctcc aagcccgctt gtcaaaaatt
       61 ctttttattg aaaaattgtt aaaactaacc ccgagggtcc caacaaaagt atttccaccc
      121 caacccccgg ctatttcaaa ctgactgttt gacttttcaa caacattata agtaatgtct
      181 acagtgttgt catgttgatt aggtactata ttgatcgcat cggcagactg aagtttttct
      241 ggctcgaaat gtcccatatt tgccaactct ctcaatgagc gctcaatatt tgactggctg
      301 taaagatatc caggtcttgt ataaagttct ctccgtacta ccttttcgtt tgttacagtg
      361 ttgccactaa tcattatcct gttgaatatg gccggtttac cctcatatat tctcatctca
      421 acatccaccg aatcattctc tatcctaacc tcaacaggtg ttacattgaa aaataaataa
      481 ccattatcag tataaagctt gctgacatta cccttttcat ctcttgtgag tctctcctcc
      541 attgaaacca tatcataaat atcacccttc tttatcctga gtatactgtt aagaaaatca
      601 cttgaatatt gtgaattgcc tgtccacgtg atatttctaa aataatattg cctgccttca
      661 tctatagtaa tatctattcc caatctgcca ttccttacat aataaatgct gtccttcact
      721 attttagcgt cacggaaacc tttctcatta tacactttga tgatgttttt cttatcgttt
      781 tcgtactctt tttcgttaaa ttttttgctt gacaacacat taagaactct gctttttgct
      841 cttgtctttt tcatggagcc ggcaagtttt gagtctttta tgttttcatt tccattgaaa
      901 tttatgtctt ggattttaac tttttgtttc cggtctacta cgaaggtcac tctaattgca
      961 ttatttattg tcgtatcctg cactttaagc actttaacct cagtttgaag aaaaccttta
     1021 tcaacataat attttttgat aatgtcacaa gccgatttct caagatagtc cgacaattct
     1081 cctcctcgct taatcttaag cttatcaata agttctgtcc tctccgacga ctttactcct
     1141 tcaaattcca acatggaaat acgtggtctc tcttgaagat aaaggttcaa ataaaccttt
     1201 ccatttgaga cagaatctat gtaaaaagcg atttcagaaa accatctctg ttgccagatt
     1261 cttttaataa tagatgatag ctcctctccc ggaatctcaa ttttatctcc tatctgtatt
     1321 cctgttaagg agagtatctg atctggattg aggtatttta ttcctgaaac tgtcagcccg
     1381 ccaatttcat aaacctgtgg cctgttataa tcgacttcca cagttcgggc gaaagtcgaa
     1441 tcctgtgcct ggacccgaat cgaaaataaa aaacatgtta agaaaaaaag tatcggaaaa
     1501 aaggat
//
LOCUS       DAOTQL010000264         1444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_73289_length_1444_cov_3.486681, whole genome shotgun sequence.
ACCESSION   DAOTQL010000264 DAOTQL010000000
VERSION     DAOTQL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73289_length_1444_cov_3.486681"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="P5293_09370"
     CDS             <1..788
                     /locus_tag="P5293_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRR50143.1"
                     /translation="YIDQSPVGKSTRSNPVTYIKAYDEIRKLFSEQPYAKMNGYGHSH
                     FSFNIDGGRCPECQGEGVITIEMQFMADVKMVCESCGGKRFKPDILEVKYYGKNINDI
                     LNMSVEEAINFFMAQKEPISHRIADKLQPLASVGLSYVKLGQSSSTLSGGESQRVKLA
                     SFLGKDSSNDSTLFIFDEPTTGLHFYDIKKLMDSFDALIAKGHSVIVVEHNMDVVKCA
                     DWIIDLGPEGGDAGGYVNFEGRPEELIKIEHNYTARALRMKMQ"
     gene            complement(769..>1444)
                     /locus_tag="P5293_09375"
     CDS             complement(769..>1444)
                     /locus_tag="P5293_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRR50144.1"
                     /translation="IGRNIIGVVRAKYYSEKYLIISAHYDHLGILEGKIYNGADDNAS
                     GVAAMLALSKYFSQLRNEGKGLTVNLIFAAFDGKEYNMAGSENFINNLPVSKKNIICD
                     INIDQIGSIFAPPSKDSNYILVLGTKSYPRLKSTIEAANNKMKYPLEINYDFYGSKVF
                     AELFYKISDQNSFVSVGIPSLFFTSGIHMHTYKPTDDYYFINFPVLFNRTVLIKNIVE
                     IIASSF"
BASE COUNT          458 a          227 c          304 g          455 t
ORIGIN      
        1 aatacataga tcaaagtcca gtaggaaaat ctacaagatc taatccagta acgtatataa
       61 aggcatatga tgagattagg aaactctttt cggaacaacc ttatgcaaag atgaatggat
      121 atggtcattc tcatttttcg tttaatattg acggaggcag gtgccccgaa tgtcagggtg
      181 agggtgttat aactatcgag atgcaattta tggcagatgt taagatggta tgtgagtcat
      241 gtggagggaa aagatttaaa ccagacattc ttgaggtcaa gtattatggg aaaaatatta
      301 acgatatttt gaatatgagc gtagaagagg caattaactt ttttatggct caaaaggagc
      361 cgatatcaca tagaattgcc gataaacttc aaccacttgc ctccgttggt ctttcttatg
      421 tgaaacttgg acagagcagc agcacactta gtggtggaga gagtcagaga gtgaaacttg
      481 cttcgttttt gggaaaagat tcttcaaacg attctacact ttttattttt gatgaaccta
      541 ctacagggtt gcatttttat gacataaaga aacttatgga ttcatttgat gcgcttatcg
      601 caaagggaca ttcagtaatt gttgtggagc acaatatgga cgtagttaag tgtgctgact
      661 ggattatcga tcttggaccc gagggaggag atgccggcgg atatgtaaat tttgaaggta
      721 gacctgaaga gttgattaaa atagaacaca actatactgc tagggcgctc agaatgaaga
      781 tgcaataatt tctactatat tttttattaa gacagttctg ttgaagagta cagggaagtt
      841 gataaagtag tagtcatctg tgggtttgta agtatgcatg tgaatgcctg aggtaaaaaa
      901 tagtgacgga atacctacgc ttacaaatga attttgatcg gatatcttgt aaaacagttc
      961 agcaaagacc ttgctgccgt aaaagtcgta attaatctcg agggggtatt tcatcttgtt
     1021 atttgctgct tcaatggtgc tttttaaccg aggatagctc tttgttccca aaacaagtat
     1081 ataattggag tccttgcttg gcggggcaaa aatggaacca atctgatcaa tgttaatatc
     1141 acaaattata ttctttttag aaacgggaag attattgatg aaattttcag atccggccat
     1201 attatattct ttcccgtcaa aagccgcaaa tattaaatta acagttaatc cttttccttc
     1261 atttcttaat tgagaaaaat attttgaaag tgccaacatg gctgcaactc cagaggcatt
     1321 gtcatctgca ccgttatata ttttaccttc aagaatgccg agatggtcat aatgggcaga
     1381 tataattaaa tatttttcac tgtaatattt tgctctcacc acaccaataa tatttcttcc
     1441 tatt
//
LOCUS       DAOTQL010000265         1443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_73373_length_1443_cov_3.144092, whole genome shotgun sequence.
ACCESSION   DAOTQL010000265 DAOTQL010000000
VERSION     DAOTQL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73373_length_1443_cov_3.144092"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1425
                     /locus_tag="P5293_09380"
     CDS             133..1425
                     /locus_tag="P5293_09380"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRR50145.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDVLAKDYPVVARFQGGPNAGHSLQ
                     FDDKKVVLHSIPSGVFHKGTTNIIGNGVVLDPLIFREECRNLISMGVPVNERVVISKR
                     AHLILPTHKIIDAASEQSKGASKIGSTLKGIGPAYVDKTGRNGLRVGDILSDNFKERF
                     YALKEKHINFLKQFDYDYDASEHESEWLEAAEYLKNFSIVDGEYEINNLVDNSKRVLA
                     EGAQGSLLDIDFGSYPFVTSSSTTCAGACTGLGVAPCRIGTVYGIFKAYCTRVGSGPF
                     PTEQNNATGELLRTLGSEFGATTGRPRRTGWLDLVALKYAVMINGVKSLVMMKADVLD
                     TIEHIKVAVAYEVDGKVTDRVPFDTYANVTPVYKEFPGWKKDLTKIKAEEDLPFEFMN
                     YVRFIEKEVGIPVNIISLGPDREQTIIRESSKTAEFFD"
BASE COUNT          429 a          264 c          338 g          412 t
ORIGIN      
        1 ttaggtagaa gaagtgagac tccgcaacaa gttcaaaatt cacacgctcc agcacctttt
       61 tcagtttctg aagaagatct ccctgatgac agtctgccat tttaatttat attaagtaat
      121 aactggtaac atatgaaagt tgatgtaatt ctcggactcc aatggggaga tgaaggtaag
      181 ggcaagattg tggatgtctt agcaaaagat tatcctgtgg ttgcaaggtt tcagggtggc
      241 ccaaatgcag gacactcctt gcagtttgat gataaaaaag ttgttcttca ttctattccc
      301 tccggagttt ttcataaagg tacaaccaat ataatcggta atggcgtggt acttgatcct
      361 ctaatcttca gggaagaatg ccgcaatctc atatccatgg gtgtccctgt caatgaaaga
      421 gtcgttatat ccaagagagc tcatctcatc cttccaactc acaaaatcat tgatgctgca
      481 tcggaacaat caaagggggc ttcaaaaatt ggttcaactc ttaaaggtat aggtccggcc
      541 tacgttgata aaactgggag aaatggtctg agggttggag atattctttc ggacaacttc
      601 aaggaaaggt tctatgctct taaagagaag catatcaatt tccttaaaca gtttgactat
      661 gactatgatg ccagtgaaca tgaatcagag tggcttgagg ctgcagaata tttgaagaac
      721 ttttctattg ttgatgggga atatgagatt aacaatctgg ttgacaacag taaaagggtg
      781 cttgccgaag gtgctcaagg ttctttgctc gatatagatt tcggctccta cccttttgtt
      841 acatcttcaa gtactacttg cgcaggtgca tgtacaggtc ttggagtagc accttgcagg
      901 attggtacag tatacggtat ttttaaggct tattgtacga gagtaggcag tggcccgttt
      961 cctactgagc agaataatgc cacaggtgaa ttgctcagga ctctgggctc agaatttgga
     1021 gctactacag gcagaccccg cagaacaggc tggttggatc tggttgccct gaaatatgct
     1081 gtgatgatca acggtgtcaa gagtttggtt atgatgaagg cggatgtgct ggacactatc
     1141 gaacatatta aagttgctgt tgcatatgaa gttgacggaa aggttacaga tagagtgcct
     1201 tttgacacat atgccaatgt cactcctgtt tacaaagaat tccccggatg gaaaaaggat
     1261 ctcaccaaga taaaagcaga agaggattta ccttttgaat ttatgaatta tgtcagattt
     1321 atagagaaag aggtgggaat ccctgtcaac attatttctc ttggtccgga cagagaacag
     1381 actattataa gagaaagcag taaaactgcc gaatttttcg attaagatcc aaaacccggc
     1441 agt
//
LOCUS       DAOTQL010000266         1422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_75028_length_1422_cov_4.813460, whole genome shotgun sequence.
ACCESSION   DAOTQL010000266 DAOTQL010000000
VERSION     DAOTQL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75028_length_1422_cov_4.813460"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..911
                     /locus_tag="P5293_09385"
     CDS             189..911
                     /locus_tag="P5293_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRR50146.1"
                     /translation="MAAAERLFEVMDLDGEPYNEGLSVELDIEELKMEKIVFGYAGRD
                     KVLQDFNLCLKSGQITALCGENGSGKSTVALLLMRMLHAESGSIAADGVDINQINLQL
                     WRNIITIVPQNPGLFSGTISQNIAPGETEPDCDLILQLCRELGLLDLLKRLPLGLDTF
                     LGENGSRLSRGEQQRIAVARALYREPKILILDEATSSCDTESKNLILNSVVRQRERGC
                     IILLISHQKEDLAIADRHVNIK"
     gene            981..1169
                     /locus_tag="P5293_09390"
     CDS             981..1169
                     /locus_tag="P5293_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50147.1"
                     /translation="MKELVEREWEVEALDSEGMQVQGGLTFRELLNLLEKAAALAEEL
                     EKYWPRFRRGFVAGWEAA"
     gene            1169..1390
                     /locus_tag="P5293_09395"
     CDS             1169..1390
                     /locus_tag="P5293_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50148.1"
                     /translation="MKTNTLFDRGELVMISYQESLGIIGGAGEGWKYDMAEYVGMGVG
                     YGAKKLWKLFKVISNNIYSMRSNPVLLYQ"
BASE COUNT          445 a          243 c          334 g          400 t
ORIGIN      
        1 tttcatttgc gggatcaact gactttaact caaagttgct ttcaattacc accttgtggg
       61 caggcgggct ttttattctt gccgacaagc ttacacttgg agaattaatt ttattctaaa
      121 cgctcacagc attattttct tctcccttaa acagtttcat cgctctcaac acatctatca
      181 gagacggaat ggcagctgcc gaaagacttt ttgaagttat ggatcttgac ggagaacctt
      241 ataatgaggg tttatcagta gaactggata tcgaggagct taaaatggag aaaattgtgt
      301 ttggctatgc aggaagggac aaagtgttac aagatttcaa tctttgtctt aaatctggac
      361 aaattacagc tctttgcggg gaaaacggaa gtggtaaaag tactgtagcg ttgctgttga
      421 tgagaatgtt acatgctgaa tcaggatcta ttgctgccga cggtgtagat ataaatcaga
      481 taaatcttca attatggaga aatataataa caattgttcc tcaaaatccg ggactatttt
      541 caggaactat cagtcaaaac atcgctccgg gagaaacaga acctgattgt gatcttattc
      601 tccaattatg cagggaactt ggtcttcttg acctccttaa aagactgcca cttggtcttg
      661 atacgttcct tggagagaat ggctcaaggc tctcgagagg tgaacagcag agaatagccg
      721 tagcaagagc tctttacaga gaacccaaaa tattgatttt ggatgaggca acttcctcgt
      781 gcgatactga atctaaaaat ttaatattaa attcagtagt cagacaaaga gaaagaggtt
      841 gcattatcct gcttatctca catcagaaag aagaccttgc tattgctgac agacatgtaa
      901 acattaaata aaaaatggag ggtgcacact cctggtgaaa ctcgcacacc gttttgcgag
      961 aaatataaaa ataatctgtt atgaaagagt tagttgaaag agaatgggaa gttgaagctc
     1021 tggacagtga aggaatgcag gttcagggcg gacttacatt cagagaattg ttaaatctgc
     1081 ttgaaaaggc tgctgctttg gcagaggagt tggagaaata ctggccgcgt ttccgccgag
     1141 gatttgttgc cggatgggag gctgcgtgat gaaaaccaat acgttgtttg acagaggcga
     1201 acttgttatg atctcttatc aggagagcct cggcatcatt ggaggtgcag gtgaaggatg
     1261 gaaatacgac atggccgagt atgtcggtat gggtgtagga tacggggcga aaaagctttg
     1321 gaagcttttt aaagtaataa gtaacaatat ttactcaatg agatctaatc ctgtattatt
     1381 gtaccagtga caaaaaaaat aatttaaaaa tcattgaccg gg
//
LOCUS       DAOTQL010000267         1388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_77831_length_1388_cov_6.011253, whole genome shotgun sequence.
ACCESSION   DAOTQL010000267 DAOTQL010000000
VERSION     DAOTQL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77831_length_1388_cov_6.011253"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="panB"
                     /locus_tag="P5293_09400"
     CDS             complement(<1..596)
                     /gene="panB"
                     /locus_tag="P5293_09400"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRR50149.1"
                     /translation="MSVEGKNRIITTQRLLEMKHNGEKIAMLTAYDYTFAKIIDEAGI
                     DIILVGDSAANVMAGHETTLPITIEQMIYHAQSVVRAVKNPMVVVDMPFGSYQGNSKL
                     AVSNAIKIMKETEADAIKVEGGSEIIDSVLTIISAGIPVMGHLGLTPQSIHKFGTFSV
                     RAKEEAEAKKLLEDAVLLEQAGCFAIVLEKIPAELGKKV"
     gene            766..>1388
                     /locus_tag="P5293_09405"
     CDS             766..>1388
                     /locus_tag="P5293_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRR50150.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPTVIINSEGKRTTPSVVAFTE
                     NGERKVGDPAKRQAITNPTRTIYSIKRFMGEPYDRVTAEIARVPYKVERGDNNTTRIN
                     IDGRLYTPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFSDSQRQATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKTHKDMKVAVFDLGGGTFDISILELGDG"
BASE COUNT          449 a          277 c          248 g          414 t
ORIGIN      
        1 acctttttgc ccaattctgc aggaattttc tctaaaacaa tagcaaaaca tcctgcctgc
       61 tcaagcaaaa ctgcatcttc aagaagtttt ttagcttcag cttcctcctt tgcacgaaca
      121 ctaaatgtcc caaacttatg aattgattga ggagttaatc caagatgccc cataacaggg
      181 ataccggcag atataatagt aagcacggaa tcaataatct cagatcctcc ctccacttta
      241 atggcatcag cttctgtctc tttcattatc ttaattgcat ttgaaacagc cagttttgaa
      301 ttaccctgat aacttccaaa aggcatgtca actacaacca tgggattttt aacagctcta
      361 accacacttt gagcatgata aatcatctgt tcaatagtta ttggaagagt agtttcatga
      421 cctgccataa cgttcgcggc tgagtctcca actaaaataa tatcaattcc tgcctcgtca
      481 atgatcttgg caaaagtata gtcataggct gtcagcatag ctattttttc accattgtgc
      541 ttcatttcca acaatctctg tgttgttatg attctgtttt taccttccac agacatgatc
      601 tgtaatttta taattaatgt ttttctaatg tccaacaaag ctatataatt atttcgaatt
      661 gactttatga caccttgtca gtttttcctt ttggcacaat gttagatgta tttcatacag
      721 actttataaa aattaatttt tgacaacaat taaaatataa atattatggg aaagataatt
      781 ggaattgacc ttggtacaac aaactcctgc gttgccgtaa tggagggaaa tgaacctaca
      841 gtcataatta acagcgaagg caaaagaacc acaccttcgg ttgttgcttt taccgaaaat
      901 ggtgaaagaa aagttggtga tcctgcgaaa agacaggcta taacaaatcc aacaagaacc
      961 atctactcaa ttaagagatt catgggtgag ccttatgaca gggttacagc tgaaattgca
     1021 cgtgtcccat ataaggttga aaggggcgat aataatacaa cccgtataaa tattgatgga
     1081 cgtctttata ctccacagga gatatcagca atgattcttc aaaagatgaa aaagacagcc
     1141 gaagattatc ttggacaaga ggtcactgaa gctgtaatta cagttcctgc atatttcagc
     1201 gattctcaaa gacaagccac aaaagaagcc ggagagatag caggtctcaa ggttagaaga
     1261 attatcaacg aacctacagc tgctgctctc gcatatggtc ttgacaagac tcataaagat
     1321 atgaaggttg ccgttttcga ccttggtgga ggtacattcg atatatctat actcgaattg
     1381 ggtgatgg
//
LOCUS       DAOTQL010000268         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_78226_length_1384_cov_2.772009, whole genome shotgun sequence.
ACCESSION   DAOTQL010000268 DAOTQL010000000
VERSION     DAOTQL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78226_length_1384_cov_2.772009"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="P5293_09410"
     CDS             complement(<1..553)
                     /locus_tag="P5293_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRR50151.1"
                     /translation="MEVNLSKKIILLLGALLMSIAVWAQAAFTVDVPKVVGLDENFRL
                     VFTATGEPSNFNPPEFKDFDVLAGPSTSTMTSTQIINGKRTDSYQVSYTFILQPKSTG
                     TFSIPSASVTISGKVYHTNPVSIEVVKGEGSSASAPLGNATSSSGTSISSDDIFMKMS
                     VSKGKVVKGETLVATLKLYTKVPI"
     gene            complement(566..1273)
                     /locus_tag="P5293_09415"
     CDS             complement(566..1273)
                     /locus_tag="P5293_09415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRR50152.1"
                     /translation="MRGKILTILFLFLYSISWGQADKWKVRQGNASYEKGKYQDAELE
                     YRRALEKNPKSTKASYNLSNTMYRLNKPAEAEKLMMKVADSVDDRGIKSAAYHNLGNF
                     NLAQKKYQEAVEAYKNSLRINPNDMETKANLAYAQKMLKNQQNNNDQNKDNKDQDKDQ
                     NKDKNKDKDKNQNDKDQNKDQNKDQNQNQQSPPKITPQTAEQMLQAIQNQEKETQDKV
                     KKEKAKVFQERQKEKNW"
BASE COUNT          352 a          288 c          236 g          508 t
ORIGIN      
        1 ctataggcac ttttgtataa agtttgagcg ttgcaacaag agtctctccc ttaactacct
       61 tccccttgct gacactcatt ttcataaaaa tatcgtctga agagatagaa gtaccgcttg
      121 atgaagttgc atttcccaaa ggagctgatg cagatgaacc ttctccttta accacctcaa
      181 tagaaacggg attagtatga taaaccttcc cgctaattgt cacagaagca gaaggtatgg
      241 aaaacgttcc tgtacttttg ggttgaagga taaaagtata gctcacctga taactgtcag
      301 ttctctttcc gttaattatc tgagtgctgg tcatcgttga tgtagatggt ccggccaata
      361 catcaaaatc cttaaattcg ggtggattga aattggacgg ctcacctgtc gcagtaaaaa
      421 caagtctgaa attttcgtcc aaccctacaa ctttcgggac atcgacagtg aatgctgcct
      481 gtgcccagac agctatcgac atgagcaaag ctccgagaag cagtattatc ttttttgata
      541 ggttaacttc catatattaa atactttacc aatttttttc cttttgtctt tcttgaaaga
      601 cctttgcctt ttctttcttc actttatcct gagtctcctt ctcttgattc tgaattgcct
      661 gaagcatctg ttctgcagtt tgaggagtta tttttggcgg agactgttga ttctgatttt
      721 ggtctttgtt ctgatcttta ttttggtctt tatcgttctg attcttgtcc ttatctttgt
      781 ttttgtcctt attttgatcc ttatcttgat ctttgttgtc cttattttgg tcattgttat
      841 tctgctgatt cttcagcatc ttctgggcat atgcaagatt tgccttggtt tccatgtcat
      901 taggattgat tctcaacgag tttttgtaag cttcaactgc ttcctgatac ttcttttgtg
      961 ccagattgaa atttcccaaa ttatgataag ccgctgattt tatccctctg tcatctacag
     1021 aatcggcaac tttcatcatt aacttttccg cttctgcagg tttgttaagc ctgtacattg
     1081 tgttactgag attataggaa gcttttgtgg actttggatt tttttccaaa gcccttctgt
     1141 attccaattc agcatcctga tacttgcctt tttcatatga ggcattaccc tgtctgactt
     1201 tccacttatc agcctgcccc catgaaattg agtataaaaa tagaaaaaga attgttaaaa
     1261 ttttacctct catgtctctc ttatttaaaa agattctttc ccttcctgtc tactaccaaa
     1321 aagtcaagaa taaataaaac aagagcaata gcaaaaaaat acatgtattg ctcgtcaaaa
     1381 tctt
//
LOCUS       DAOTQL010000269         1360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_80395_length_1360_cov_4.597701, whole genome shotgun sequence.
ACCESSION   DAOTQL010000269 DAOTQL010000000
VERSION     DAOTQL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80395_length_1360_cov_4.597701"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="P5293_09420"
     CDS             <1..591
                     /locus_tag="P5293_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRR50153.1"
                     /translation="NYSKGDIIARQGESCKGLYMLIKGSVRTEMIAENGSLLYIETID
                     AIKPLAPAFIFATVNNFPVDVIALEESKVLFISREEILKILQNDQDLLLRYLRYNSDK
                     TQFLSNKIQMLSIKTIKSKLSHYILELLKVEQKTKPGSSTIIMDKNQTELAAYFGVTR
                     PSLARALGEMARDGLISVNRKTVSIKNLKGLIETEG"
     gene            complement(599..1075)
                     /gene="nuoE"
                     /locus_tag="P5293_09425"
     CDS             complement(599..1075)
                     /gene="nuoE"
                     /locus_tag="P5293_09425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRR50154.1"
                     /translation="MGAFKLQECHKKTIADICTYYHNDPGNLIKILYSAQREFGYLPE
                     DVQREIAHILNISVARVYGVVSFYSFFTMTPKGKYPISVCMGTACYVRGGEKILDELK
                     KQLNIKVGGVTDDGKFSLDCLRCVGACGLAPVMLIGDKVYGRLEPSMIKEILDSYE"
     gene            complement(1081..>1360)
                     /locus_tag="P5293_09430"
     CDS             complement(1081..>1360)
                     /locus_tag="P5293_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HRR50155.1"
                     /translation="GIRAGLYNFHAIEIMACPGGCIGGAGQPLHHGNSDIIKARFGAI
                     YREDASKTIRKSHENPYIIKLYEEFLGHPMSEKAHHLLHTQYFEKNKN"
BASE COUNT          440 a          290 c          234 g          396 t
ORIGIN      
        1 aattattcta aaggcgatat tattgcaaga cagggagaaa gctgcaaagg tctgtatatg
       61 cttatcaagg ggagtgtcag aacagagatg attgccgaaa acggcagttt gctttatata
      121 gaaacaattg atgcaatcaa acctcttgca ccagcattca tttttgcaac agtcaacaac
      181 ttcccggttg atgtaatagc actcgaagaa agcaaagtac tttttatctc aagagaagaa
      241 atacttaaaa tacttcagaa cgatcaagat cttcttctga gatatttaag atacaactca
      301 gataaaaccc agtttctttc aaacaagatc caaatgcttt caatcaagac cattaagtcc
      361 aaactatctc actatattct tgaactctta aaagttgaac aaaaaacaaa acccggttct
      421 tccacaatta ttatggacaa gaatcagact gaacttgctg catattttgg agtaacaagg
      481 ccgtctcttg cgcgagcctt aggtgaaatg gcaagagacg gcctgatatc tgtaaacaga
      541 aaaacagtct caattaaaaa cctgaaaggt ttaatagaga ctgaaggata atctaaatct
      601 attcgtaact gtcaagaatt tccttgatca tagaaggttc cagacgtcca tacaccttgt
      661 cgccaataag cattaccgga gccaggccac aggcacctac acaccgcagg caatccagtg
      721 aaaattttcc atcatctgtc acacctccaa cttttatatt aagctgtttc ttaagctcat
      781 cgagaatttt ctcccctcca cgtacataac atgctgttcc catacacacc gaaataggat
      841 acttgccttt cggagtcatt gtgaagaaag agtaaaacga aaccactcca tatactcttg
      901 caaccgatat attgagaata tgtgcaattt ctctctgtac atcctccgga agatatccaa
      961 actctctctg agcactgtag agaattttga tcaagttacc gggatcgtta tggtaatatg
     1021 tacaaatatc tgctatagtc tttttatgac actcttgtaa tttaaatgcg cccatggtga
     1081 ttagttttta tttttttcaa aatattgagt atggagtaga tgatgagcct tctcactcat
     1141 cggatgacca aggaattcct cataaagttt tatgatataa ggattttcat gagattttct
     1201 gatcgttttg ctggcatctt ctctgtaaat tgccccaaaa cgcgctttta tgatatcaga
     1261 gtttccatga tgaagaggtt gccctgcccc accgatacat cctcccggac atgccattat
     1321 ctcaattgcg tgaaaattgt acaatcctgc acgaatacca
//
LOCUS       DAOTQL010000270         1270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_89521_length_1270_cov_5.411523, whole genome shotgun sequence.
ACCESSION   DAOTQL010000270 DAOTQL010000000
VERSION     DAOTQL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89521_length_1270_cov_5.411523"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="P5293_09435"
     CDS             complement(<1..640)
                     /locus_tag="P5293_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRR50156.1"
                     /translation="MGFELKSDYKPTGDQPEAIEQLVNYLQNGVEAVTLLGVTGSGKT
                     FTMANVIERLQRPALILSHNKTLAAQLYGEFKEFFPNNAVEYFVSYYDYYQPEAYLPT
                     TDTYIEKDLSINDEIEKLRLHTSSSLLSGRRDVIVISSVSCLYGIGNPEDFHSSTVKL
                     KKKQKISRSKLLYALVDSQYSRNEADFKRGTFRVKGDTIDIYPAYSDTACRII"
     gene            complement(648..>1270)
                     /locus_tag="P5293_09440"
     CDS             complement(648..>1270)
                     /locus_tag="P5293_09440"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRR50157.1"
                     /translation="SDNSLVISVKHSPNPTLKGMLIGLDAGHGGSASGAVSPSGIKEK
                     DLNLSMVYMLKEEFEKRGAKVVLSRKDDSDMTMAERKKIFKDAGIDMLISIHCNASGN
                     PLVSGGTSAYYKHIEYRDLTAAILKRLVAIDGGIKNFGLVGNFNFSLNGPTDYPSVLV
                     ETLFLSSLPDEEKISDPQFRRKMMIEVAEGVEDYLKIVKKEAKKEK"
BASE COUNT          374 a          287 c          201 g          408 t
ORIGIN      
        1 atataattct acatgcagta tcagaatatg caggatagat atcaattgta tcgcctttga
       61 cacgaaaggt ccctctttta aaatcggctt cgtttcttga gtactgacta tcgacaagtg
      121 catagagcaa tttgctcctt gaaatttttt gctttttttt caattttaca gtactggaat
      181 gaaaatcctc aggattcccg ataccataca aacatgagac acttgaaatc actatcacat
      241 ccctcctccc tgaaagaagt gaactggaag tgtgaagtct cagcttttca atttcatcat
      301 taatgctcag atctttttca atatatgtat cggtagtagg caaatatgct tctggttggt
      361 aatagtcata ataagaaaca aagtactcaa cggcattgtt gggaaaaaac tctttaaact
      421 ctccgtacag ctgggcagcg agagtcttgt tatgacttaa tataagagca ggtctctgta
      481 atctttctat cacattggcc atagtgaaag tcttgcctga accggtcaca ccaagcaagg
      541 tcacagcttc tacgccgttt tgaagatagt tgaccagttg ctcaatagct tcaggctgat
      601 ctcccgtagg cttatagtca gactttagtt caaatcccat cagactgtta cttctccttt
      661 ttagcctcct ttttcacaat tttcagatag tcttcaaccc cttctgccac ttcaatcatc
      721 atttttctcc tgaattgagg gtcagatatt ttctcctcat ctggcaaaga actcaaaaac
      781 aaagtttcaa caaggactga cggataatct gtcggtccat tcagagaaaa attaaaatta
      841 cctacaagac caaaattctt tattcctcca tcaatggcaa ccaacctttt aagaatggct
      901 gcagtcagat ctctatattc aatatgcttg taatatgcac ttgtgccacc acttactaat
      961 ggattgccac ttgcattaca gtgtatggat ataagcatgt ctattccggc atctttgaaa
     1021 attttctttc tttcagccat agtcatatct gaatcatcct ttctgctcaa aaccactttg
     1081 gcacctcttt tttcaaactc ctccttcaac atatatacca ttgaaagatt caaatccttt
     1141 tccttaatac cggatggact aaccgcacct gatgcacttc ctccatgccc ggcatccaga
     1201 ccaattaaca tacctttaag agtaggattt ggagaatgtt taacactaat tactaaagag
     1261 ttgtcagaat
//
LOCUS       DAOTQL010000271         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_89644_length_1269_cov_3.299835, whole genome shotgun sequence.
ACCESSION   DAOTQL010000271 DAOTQL010000000
VERSION     DAOTQL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89644_length_1269_cov_3.299835"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="P5293_09445"
     CDS             complement(<1..371)
                     /locus_tag="P5293_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HRR50158.1"
                     /translation="MKKGNKYGIHRVIEPKGVLPQPANKLDNNMDEIYDNEILIDVKT
                     LNIDSASFTQIEEQAGGDKAKIAEIMMDIVAKQGKHRNPVTGSGGMLLGVVEKIGDAL
                     IGKTDLKIGDKIATLVSLSLTP"
     gene            complement(415..1239)
                     /locus_tag="P5293_09450"
     CDS             complement(415..1239)
                     /locus_tag="P5293_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRR50159.1"
                     /translation="MEKLIITAAICGAEVLKEHNPAVPYTVEECAREAKSAYDAGASI
                     IHLHVRTDDGTPTQDKNRFKEVMDAIYKLCPDVIIQPSTGGAVGMSNDERLWPTELYP
                     EMATLDCGTLNFGGDEIFENTENTIKYFGTKMIERGIKPELEVFDKSMIDMALRLHKK
                     GYILSPMHFDFVMGVNGGISGELRDFVFLRGSIPPDATYTVAGVGRYEFPLAAAAIID
                     GGHVRVGFEDNVYLSKGVLAKSNGELVSKVVRLAGELGRDIASPSEARKILGLKEK"
BASE COUNT          366 a          287 c          178 g          438 t
ORIGIN      
        1 ggtgtaagag agagagaaac aagtgtcgca attttatcgc caattttcaa atctgtcttg
       61 ccaataagcg catctcctat tttctcgaca acacctagca acatgccacc tgatcctgta
      121 acaggatttc tgtgctttcc ctgttttgct actatatcca tcattatttc agctatttta
      181 gccttatcac ctccagcttg ctcttcaatt tgtgtaaaac tagcagaatc tatgtttaaa
      241 gtcttgacat cgataagtat ttcattatca tatatctcat ccatgttatt atcaagcttg
      301 ttagccggtt gaggtagaac tccttttggt tctatgactc tgtgaatccc atatttatta
      361 ccttttttca tattaattta ttattaaatt attattttaa ttttaatatt ctttttactt
      421 ttcttttaat cctaaaattt tgcgggcttc agaaggtgat gctatgtcac gacccaattc
      481 tccggccaat cttacaactt ttgaaaccag ctccccattt gatttcgcaa gaactccctt
      541 ggacaaataa acgttgtctt caaatcctac ccttacatgg ccaccatcaa taatggcagc
      601 agcagcaagt ggaaattcat atcttccaac tcctgcaact gtataagtag catccggagg
      661 aatactgcct ctcaaaaaaa cgaagtctct taattctcct gaaataccac cattcactcc
      721 cattacaaag tcaaaatgca tagggcttag tatatatcct ttcttgtgaa gacgcaatgc
      781 catatcaatc atacttttgt caaatacttc gagttccggc ttaattcccc tttcaatcat
      841 ttttgtccca aaatacttaa tagtattttc agtattttca aagatttcat ctcctccaaa
      901 attaagagta ccacaatcaa gagtagccat ttcgggataa agttctgtag gccaaagtct
      961 ttcatcatta gacataccta cagcaccgcc agtagacggc tgaataataa catcaggaca
     1021 caatttatag attgcatcca ttacctcctt aaatctattt ttatcctgag taggagttcc
     1081 atcatctgtt ctaacatgaa gatgaattat acttgcaccg gcatcgtatg ccgatttagc
     1141 ctctcttgca cactcctcta ctgtataagg gacagcagga ttgtgctctt tgagcacttc
     1201 tgccccgcat atagcggctg taataattaa cttttccata taattataat ttttataatt
     1261 atctatttt
//
LOCUS       DAOTQL010000272         1238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_93206_length_1238_cov_3.202874, whole genome shotgun sequence.
ACCESSION   DAOTQL010000272 DAOTQL010000000
VERSION     DAOTQL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93206_length_1238_cov_3.202874"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..>1238
                     /gene="dxs"
                     /locus_tag="P5293_09455"
     CDS             53..>1238
                     /gene="dxs"
                     /locus_tag="P5293_09455"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRR50160.1"
                     /translation="MEILNRINNPSDLKKLNISELHKLCDELREYLIECCANNPGHVA
                     SSLGTVELAVALHYVYDTPEDKLVWDVGHQAYAHKILTGRRDAFVNNRKYKGISGFPK
                     ISESEYDAFGVGHSSTSISAALGMSVAFTLQGKNNKVVAIIGDGAMTGGLAFEGLNNA
                     GAMKSDILVILNDNGISIDPNVGALHNYLLKISTSRTYNRFKNKIWELVGSNRLRKFF
                     QKIIFSTKMAFFKSGSIFESLGFRYFGAIDGNNLDQLITTLSNLKEIKGPKLLHIVTT
                     KGKGYKPAEDNQIEWHAPGAFDIDTGAILKNPAEKRTKFQDVFGETIVKLARENKKIV
                     GITPAMPTGCSLNLMMKEFPERVFDVGIAEQHAVTFSAGLASQGMLPFCNIYSSFMQR
                     AYD"
BASE COUNT          379 a          240 c          271 g          348 t
ORIGIN      
        1 aatgatggca aatattttgt aactttcgga gttggaaaag tgagaattta tcgtggaaat
       61 ccttaataga attaacaatc cttcagacct taaaaaatta aatatcagcg aactccacaa
      121 gttgtgcgat gagttgagag aatatctcat agagtgttgt gcaaataatc ccggtcatgt
      181 tgcttcaagt cttggcactg ttgaactcgc ggttgcactc cattatgttt acgatacccc
      241 agaggataag ttagtatggg atgtgggtca ccaggcctac gcccataaaa ttttgacagg
      301 cagaagagat gcttttgtca ataacagaaa atacaaaggt ataagcggat tcccaaaaat
      361 aagcgaaagt gaatatgatg cttttggcgt agggcactct tctacttcaa tctctgcagc
      421 ccttggtatg tcagtggctt ttacacttca aggtaaaaac aacaaggttg tggcaattat
      481 tggtgatgga gccatgacag gaggtcttgc ttttgaaggt ctcaacaatg ccggtgcaat
      541 gaaatcggat attctggtta tcctcaatga taacggcata tccattgatc ccaatgtcgg
      601 tgcgcttcac aactatctgc ttaaaatatc tacttctcgc acatacaaca gatttaaaaa
      661 caaaatttgg gagctggttg gttccaacag actgagaaaa ttttttcaga aaattatctt
      721 cagcactaaa atggctttct tcaaaagcgg ctcaatcttc gagtctctcg gatttaggta
      781 ttttggtgca atcgacggga acaatctcga tcagcttata actacactga gcaatctcaa
      841 agagataaaa ggccctaaat tgcttcatat agttaccaca aaaggtaagg gatacaaacc
      901 agccgaagat aatcagatcg aatggcatgc tcccggggct ttcgatattg ataccggagc
      961 tattctgaaa aatcctgccg agaagaggac aaagtttcag gacgtctttg gcgagactat
     1021 agttaagctt gccagagaga acaagaagat tgtgggtatt actcctgcca tgcctactgg
     1081 atgctctctc aatctaatga tgaaggagtt tcctgaaaga gtatttgacg ttggcattgc
     1141 agagcagcac gctgttacat tttctgcagg gctggcctcc cagggtatgc tgccattttg
     1201 taatatctat tcaagcttta tgcaaagggc atatgaca
//
LOCUS       DAOTQL010000273         1233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_93793_length_1233_cov_3.182513, whole genome shotgun sequence.
ACCESSION   DAOTQL010000273 DAOTQL010000000
VERSION     DAOTQL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93793_length_1233_cov_3.182513"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="P5293_09460"
     CDS             complement(<1..222)
                     /locus_tag="P5293_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50161.1"
                     /translation="MGDLMLFFILTASGLLLAGGMYFIFAEALYLPRIATTKAVLNVA
                     RQDKKQTKNMEAIIFELASKLSKFIRMDDY"
     gene            complement(210..995)
                     /locus_tag="P5293_09465"
     CDS             complement(210..995)
                     /locus_tag="P5293_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRR50162.1"
                     /translation="MKKKYDEAYASSFIKGLTRLTGISESKLKKYAAENNLFNVLEHP
                     NIIEPNKQQLEKIHVLNEFISTYKLLKLQEKENKIVLNSSTVAGEYFVSLLGGIKDRE
                     KFMAAFLDSGNNIIETRTLSEGSIGEAVVYPRMVLKAALDCDCKSIILAHNHPGGSLN
                     PSIQDRDITEKLVSIFAPLQISVIDHIIVANINYYSMAEKGNIPLPSPKSISYDAVPL
                     SDGKIVGEETMGFNADTLDKDDLELDSMYFFEQDGEEEEEWEI"
     gene            complement(992..>1233)
                     /locus_tag="P5293_09470"
     CDS             complement(992..>1233)
                     /locus_tag="P5293_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50163.1"
                     /translation="GRLFEVDSDITEQISRCIQYYGAVEFFKNLESFDFSADVFEKLD
                     AVRLVLFGMGEEVMPELLTDEIRTQIDSTEGGAEQ"
BASE COUNT          338 a          289 c          212 g          394 t
ORIGIN      
        1 gtaatcatcc atccttataa atttagaaag cttggaagcc aattcaaaaa taatggcttc
       61 catgtttttt gtctgctttt tatcctgcct tgcaacattt agtactgcct tggttgtcgc
      121 tatccttggc aggtaaagtg cctctgcaaa gatgaaatac atacctcctg ccagaagcaa
      181 cccggaagct gtcaaaatga agaacaacat caaatctccc attcttcttc ctcctctcca
      241 tcctgttcaa aaaaatacat gctgtccagt tctaaatcat ctttgtccaa cgtgtcagca
      301 ttgaatccca ttgtttcttc tccgactatc ttcccatcac tcaaaggaac cgcatcataa
      361 cttatacttt tgggagatgg taggggaata ttcccctttt cggccatgga gtagtaattg
      421 atatttgcaa cgataatatg gtctatcacg ctgatttgaa gtggtgcaaa gatggaaacc
      481 aatttttctg tgatatctcg gtcttggatt gaaggattca ggcttccccc aggatgattg
      541 tgtgctaaaa tgattgattt gcagtcacag tcaagagcag ccttcagaac catcctcgga
      601 taaacaactg cttctccgat acttccttca gataatgtcc ttgtttcaat gatgttgttg
      661 ccgctatcca aaaaggctgc cataaacttc tctctgtctt taatgccgcc caataaggaa
      721 acaaaatatt ctcctgctac cgttgaagag tttaacacaa tcttgttctc tttctcctgt
      781 aatttgagaa gcttgtaggt agatataaac tcatttaaga catggatctt ttcaagctgc
      841 tgtttgttag gctcaataat gttgggatgc tctaaaacat taaatagatt gttctcggct
      901 gcatattttt tgagtttact ttcagaaata cctgtaagcc tcgtcaaacc tttaatgaaa
      961 ctggacgcat aagcctcatc atactttttc ttcactgctc tgcaccacct tccgtgctgt
     1021 caatctgtgt tcttatttca tcggtcagca actccggcat tacttcttcg cccataccga
     1081 acagcacaag cctgacagca tccagctttt caaagacatc agctgaaaag tcgaaggatt
     1141 caagattttt aaaaaattcc actgctccat agtactggat acacctgctg atttgttctg
     1201 taatgtccga gtcaacttca aagagcctcc cta
//
LOCUS       DAOTQL010000274         1212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_96418_length_1212_cov_2.636992, whole genome shotgun sequence.
ACCESSION   DAOTQL010000274 DAOTQL010000000
VERSION     DAOTQL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96418_length_1212_cov_2.636992"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="P5293_09475"
     CDS             <1..310
                     /locus_tag="P5293_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRR50164.1"
                     /translation="RMNTTRKKHSAAFKAQVAIEAIKEQETLSELAKRFEVHPQMISN
                     WKREFLSRSSEIFSTKAPDEEAHRREQALYEKIGRLEIEVDFCKRASEKLGILKSSKK
                     "
     gene            361..534
                     /locus_tag="P5293_09480"
     CDS             361..534
                     /locus_tag="P5293_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50165.1"
                     /translation="MNRSTHYYKPVEEDPEDLDVMRKMDEEYLEHPTKGVLGMVDFIK
                     KNGCYGHLSGEKP"
     gene            553..1032
                     /locus_tag="P5293_09485"
     CDS             553..1032
                     /locus_tag="P5293_09485"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRR50166.1"
                     /translation="MHSNQVWSIDITYIPMEHGFLYLTAIIDVYSRYIVGWSLSNTLG
                     AECSLNVLKQAIAEFGKPEIINSDQGSQFTCPLWTKYLEEQGITISMDGRGRALDNIY
                     IERFWKTIKQEYIYIWPAENGNTLVRGIKEYMNYYNNQRTHQNLDRLTPFDWYEFAA"
BASE COUNT          388 a          213 c          292 g          319 t
ORIGIN      
        1 tcgtatgaac acaaccagaa aaaaacacag tgcagcattt aaggcgcagg tagccataga
       61 ggcaatcaag gaacaggaga cactcagcga gctggcaaag cggtttgagg tacatcccca
      121 gatgatctcg aactggaaac gggagtttct gtcccggagt tcggagatat tttcgaccaa
      181 ggcacccgac gaggaagccc ataggcgaga acaggcacta tatgagaaga tagggagatt
      241 ggagatagag gtggattttt gcaagagggc ctcagaaaag ctggggatac tgaagtcctc
      301 aaagaaatag tatcccctgg agaaaggctc agtatccgtc gacaatgtga actttttggt
      361 gtgaacagaa gcactcatta ctataaacct gttgaagagg atcctgaaga tcttgatgtg
      421 atgcgcaaga tggatgagga gtatcttgag catcctacta agggagttct tggcatggtt
      481 gattttatta agaaaaatgg gtgttatggc catttatccg gggaaaaacc ttagtaagtt
      541 gggaacggca aaatacattc aaatcaggtc tggagcattg acattacata tattccaatg
      601 gaacacgggt ttctttatct tacggcaatc attgatgttt acagccgcta tattgttgga
      661 tggagtctct ccaacactct tggtgccgaa tgttccctta atgtgctcaa acaggctatt
      721 gccgagtttg gcaagcctga gattataaat tctgaccagg gttcacagtt tacctgtccc
      781 ttatggacaa aatatcttga agagcaagga atcacgatta gtatggatgg cagaggcagg
      841 gcgcttgata acatttacat agaaagattt tggaaaacaa ttaaacaaga gtacatttac
      901 atttggccgg ctgagaatgg aaacacactt gtaagaggta taaaggaata tatgaactac
      961 tacaataatc agcgaactca ccaaaatctt gacagattga caccttttga ttggtatgaa
     1021 tttgcagctt agaatttaaa ctaaataacc attacccgtg gttggaaatt ggggagtatt
     1081 atacttttac cagggtcttc agagtttggt catgcattcg agcactttat catacaggaa
     1141 ataattgctt acatattcag gatacgaagt agatgcagtt attgggaatg acaatgttgc
     1201 tatcgaaatt aa
//
LOCUS       DAOTQL010000275         1179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_100689_length_1179_cov_3.809609, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000275 DAOTQL010000000
VERSION     DAOTQL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100689_length_1179_cov_3.809609"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1179
                     /locus_tag="P5293_09490"
     CDS             <1..>1179
                     /locus_tag="P5293_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRR50167.1"
                     /translation="IRNLGAKVKVEKCKFLRQKVEIVSPGIEEIRKQLSKSDIFYRSN
                     KSLELCVGESGLLARLLIPIAGHFLSSDSKGVQSVKITGKGTLNNRIMFYPATDPFPE
                     IGLQIESNNDRLPATIKGHIKEGNLKLLGGGGSQLLSGLLMSLPMCEKNSTIEVKEPT
                     STPYVDLTIQTLKDFGVVVANHDFHEFKIPGKQRYKARPFYPIEGDWSGASMLFVAGA
                     ITNGVSITNLPVNSKQADEKIISILRGCGVDINIVEYPKYLVMCGDVPCAFKEDSTKE
                     VILGSKIDLLKPKGSLLPFEFDATNAPDLFPSLVVLALNCEGESRIKGIKRLSNKESN
                     RAESLYTEFTKLGADIDLDGDWMIVKGAQLHGGFCSSHNDHRIAMAIITAGLKIKENV
                     "
BASE COUNT          356 a          190 c          272 g          361 t
ORIGIN      
        1 aatcagaaat ttaggtgcaa aagtaaaagt tgaaaaatgc aagtttctca gacaaaaggt
       61 tgagattgtg agtcctggta tagaggagat taggaaacaa ctgtccaagt cagatatttt
      121 ttaccgttcc aataagtctc ttgaactttg tgttggtgaa tcaggattat tggctcgttt
      181 actgattcct attgctggtc attttctcag ttctgacagc aagggagtgc aatccgttaa
      241 aattaccggg aaggggactc taaacaacag aattatgttt tatcctgcta ctgatccttt
      301 tccagagata ggtcttcaga ttgaatcaaa caatgacagg ttgcctgcaa caatcaaagg
      361 ccatattaag gagggtaatt taaaattgct tggaggagga ggttctcagc tgctttcagg
      421 attactcatg tcgctcccta tgtgtgaaaa gaacagtaca attgaggtta aggagcctac
      481 gagtactcct tatgtcgatt taactattca aactctcaag gattttggag ttgttgttgc
      541 taatcatgat tttcacgaat ttaaaatacc tggtaaacaa agatataagg cacgtccatt
      601 ttatcctatc gagggggatt ggagtggggc atcaatgctt tttgttgcag gagcaattac
      661 caacggtgta tctataacta accttcctgt caattccaag caggcagatg aaaaaataat
      721 atctatcctt agaggttgtg gtgttgacat taatattgtc gaatatccta aatatctggt
      781 gatgtgtggt gatgttccgt gtgcgtttaa ggaagacagc acaaaagagg ttattctcgg
      841 ttctaagata gatcttctta aaccaaaagg cagccttttg ccgttcgagt ttgatgccac
      901 taatgctcct gatctttttc cgtctttagt ggttcttgca ttaaactgcg agggggagag
      961 caggataaag ggaattaaaa ggttgtcgaa taaagagagt aacagagctg aaagtttata
     1021 tacagagttt acgaaactgg gagctgatat agatttagac ggagattgga tgattgtgaa
     1081 gggcgctcag cttcatggtg gtttttgctc atctcacaat gatcacagaa tagctatggc
     1141 aatcattact gcagggttga aaatcaaaga aaatgtttt
//
LOCUS       DAOTQL010000276         1157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_103798_length_1157_cov_3.896552, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000276 DAOTQL010000000
VERSION     DAOTQL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103798_length_1157_cov_3.896552"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="P5293_09495"
     CDS             <1..470
                     /locus_tag="P5293_09495"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRR50168.1"
                     /translation="IISDSINIALERTKGVIAVIENTAGQGSNLGHTFEQIAQILYYV
                     EDKSRVGVCIDTCHSFAAGYDLSTPEGFTDTFNKFEKTIGLEYLKGMHLNGARKGLNS
                     HIDRHEPIKEGVLGTDVFKLIMRDTRFDNMPLILETPNESLWKEEIALLKSWV"
     gene            complement(477..>1157)
                     /locus_tag="P5293_09500"
     CDS             complement(477..>1157)
                     /locus_tag="P5293_09500"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF015883.2"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HRR50169.1"
                     /translation="SLLEWCVREDLNKRSNRYMAVLNPVKLVIVNYPEDMVETMTAPL
                     NPDHPDGPSRKIPFSKELYIERDDFMENPPKKYFRLQPGGEVRLKYSYIIKCQEVIKD
                     SEGNVVEIRCSYDPSSRPGAGEWRSVKGTIHWVSIKHSQCAEVRLFDRLFTLADMDTV
                     PEGEQYTDYLNPHSLELTVAYCEPNLFEDKSGIAVQFERIGYFIKDNDSTADKVIFNR
                     TVTLKSAW"
BASE COUNT          367 a          231 c          218 g          341 t
ORIGIN      
        1 gaataatttc tgattcaatt aatattgcac tcgaacgaac aaaaggagta attgctgtta
       61 tagaaaatac agcaggacaa ggcagcaacc ttggccacac atttgaacag attgctcaaa
      121 tattgtacta cgttgaagat aaaagcagag tcggagtttg tattgacaca tgccattcat
      181 ttgcagctgg gtatgatttg tctactccgg aaggttttac agacactttc aacaaatttg
      241 aaaaaacaat cggtctggaa tatttgaaag gcatgcatct caatggagcc aggaaaggcc
      301 ttaactctca tatagacaga catgagccca taaaggaggg agtgcttgga actgatgttt
      361 ttaaattaat aatgcgtgat acacgatttg acaatatgcc acttatactc gaaaccccta
      421 atgaatctct ctggaaagaa gagattgctc ttctcaaaag ttgggtataa aataaattac
      481 catgccgact taagagtcac agtcctgtta aaaatcacct tatccgctgt tgaatcatta
      541 tccttaataa aatagcctat cctttcaaat tggacagcta ttccggattt gtcttcaaaa
      601 aggttcggct cacaataggc tacggttagt tccaaagaat gcggattaag gtagtctgtg
      661 tattgttccc cctctgggac agtatccata tctgcaagtg taaaaagtct gtcaaacaga
      721 cgtacttcag cacattgaga atgtttaatg gatacccagt ggatggtacc ttttacagaa
      781 cgccattctc ctgctccagg acgacttgaa ggatcatatg aacatcttat ctcaacaacg
      841 ttaccctcag aatccttaat aacttcctga catttaatta tataagaata tttcaatctg
      901 acctcgcctc caggttgcaa tctgaaatat ttttttggag ggttctccat aaaatcatcc
      961 ctttcaatat agagctcctt tgaaaaagga atctttcttg aggggccatc aggatgatca
     1021 ggatttaatg gagcagtcat ggtttcaacc atatcctctg gatagttaac tattaccagt
     1081 tttacaggat ttagtactgc catgtaacga ttggatcgtt tgttcaaatc ctccctgaca
     1141 caccattcga gaagaga
//
LOCUS       DAOTQL010000277         1139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_106429_length_1139_cov_5.669742, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000277 DAOTQL010000000
VERSION     DAOTQL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106429_length_1139_cov_5.669742"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1139
                     /locus_tag="P5293_09505"
     CDS             <1..>1139
                     /locus_tag="P5293_09505"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRR50170.1"
                     /translation="MNMVNGKNVNKGAVWVAVRIPDGCISAHANQARITTFPLNDPDN
                     CLYSPDVITFARKNGYYKGDDKDFNFSEAYAPLDFGAMRGCEARVWAAFNILGGGMIG
                     EKPYTAYEDYAMGYNKTNRLPLYIRPKEKITVKMLADVMRDHYEGTVLEMTKDIGAGG
                     NELPYRWRPLTFKVDSLTYTNERAIATQQTGFWLIGQSRDWLPDEIGGVLWFGVDDAA
                     TSTLSPIYVGIQKVPECFRVGNGDMMTYSPTSAFWLFNRISNFAYSRYNLIFPEIRAA
                     IDKRERQLLHRMPVIDAAALTFYKRSPDEAREFLTDYSVNTAQEMFNTWVEFERHLLV
                     KYIDGNVKRIGPDGHFLNNGYSKAIPAPPLQPGYSEKWKRAVKEDA"
BASE COUNT          345 a          199 c          259 g          336 t
ORIGIN      
        1 atgaatatgg tgaatggtaa aaatgtaaat aaaggtgcag tatgggttgc cgttagaatc
       61 ccagatggat gtatatctgc acatgccaat caggcaagga ttactacatt tccactcaat
      121 gatcctgaca actgtcttta ttctcctgat gtaataactt ttgcacgcaa aaatgggtat
      181 tataaaggcg atgataagga tttcaacttt tctgaagctt atgctccttt ggattttgga
      241 gctatgagag gttgtgaagc aagagtttgg gctgcattca atattcttgg aggagggatg
      301 attggagaaa aaccatatac tgcttatgaa gattatgcaa tgggatacaa taaaaccaac
      361 agattgcccc tttatatcag accaaaggag aagattactg ttaaaatgct tgccgacgtt
      421 atgagagatc attacgaggg gacagtcctt gaaatgacaa aagatattgg agcgggcgga
      481 aatgaattgc cctacaggtg gagacctttg acatttaagg ttgattctct cacatatacc
      541 aatgaaaggg caattgccac acagcagaca ggtttttggt tgattggaca gtcccgtgac
      601 tggttgcctg atgaaatagg tggagttctt tggtttggag tggatgatgc cgccacttcc
      661 actctttctc ctatatatgt tggaattcag aaagttccgg aatgcttcag agtaggtaat
      721 ggtgacatga tgacctattc acctacatct gccttttggt tgtttaacag gatttcaaat
      781 tttgcatatt ccagatataa ccttattttt ccagaaattc gtgcagcgat tgacaaaaga
      841 gaacgtcaac tgcttcatag aatgcctgta atagatgctg cagccctcac tttctataaa
      901 agatcgcctg atgaagccag agagtttttg acagactatt ctgttaatac tgctcaggag
      961 atgttcaata catgggttga atttgaaaga catctcctgg taaaatatat tgatggtaat
     1021 gtcaaacgaa tcgggccaga tggtcatttt ctgaataatg gttattccaa agcaatccct
     1081 gcacctcctt tgcagccggg ttattctgaa aaatggaaga gagcagtcaa agaagatgc
//
LOCUS       DAOTQL010000278         1139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_106431_length_1139_cov_4.939114, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000278 DAOTQL010000000
VERSION     DAOTQL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106431_length_1139_cov_4.939114"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="P5293_09510"
     CDS             <1..619
                     /locus_tag="P5293_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRR50171.1"
                     /translation="AEMIARAGIGAMYVIDPDIVTYSNKNRQLLALESTIGMKKVDVI
                     SSRLRDINPEIDIKTVADFLNEDNVALLLGGEKIDYIVDAIDTLSPKIALIKYALKNG
                     IPIVSSMGAGAKYDITAIEIADISKSYNCPLAYMLRKRLRKEGISSGFKVVFSSELPD
                     KNAIVPVEEQNKKSVVGTLSYLPAAFGIACAQVVICYFIDISKKN"
     gene            667..>1139
                     /locus_tag="P5293_09515"
     CDS             667..>1139
                     /locus_tag="P5293_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRR50172.1"
                     /translation="MKVEIREAVTTSEIKKFVCFPHKLYKNCKQYVPVLDKDEIRSLT
                     ESPSLRYCKLRLWLAYSDHNRIVGRIAGIYNPRANEYHNNKRIRFGWFDFVEDIEVAA
                     ALLGKVEEWGVSLGMEEIHGPLGYNTWYRQGVLIEGFENTPPANCLYNYPYYRSIL"
BASE COUNT          360 a          170 c          255 g          354 t
ORIGIN      
        1 tgccgaaatg atagcgagag ccggtatagg ggctatgtat gttattgatc cggatattgt
       61 gacatattca aataaaaacc gccagttgct tgctcttgaa agcacaattg ggatgaaaaa
      121 agtggatgtg atatcttccc gattgagaga tataaaccct gagattgaca ttaagactgt
      181 tgcagatttt cttaacgaag ataatgttgc tcttttgctt ggaggagaaa agattgatta
      241 tattgtggat gcaattgata cgctgtctcc aaagatagca cttatcaaat atgctttaaa
      301 aaatgggatt ccgattgtca gctccatggg agccggtgcc aaatatgaca taacagcaat
      361 tgagattgct gatatatcaa aatcttataa ctgtccgctg gcttacatgc tgaggaaaag
      421 attgaggaaa gagggaatca gtagcggatt caaggtggtg ttttctagtg aattacctga
      481 taaaaatgcc attgtgcctg tagaagaaca aaataaaaaa tcagtggtag gtactttatc
      541 gtatctacct gcagcatttg ggattgcatg tgcacaggtg gtaatttgtt actttataga
      601 tatctccaaa aaaaattaaa attattaatt acatttgttg cctttaaaca ttaatactga
      661 ccgggaatga aagttgaaat tcgtgaggcc gtaactactt ctgagataaa aaaatttgtc
      721 tgttttcccc ataagcttta taaaaattgc aaacaatatg tacccgttct tgataaggat
      781 gaaattcgat cattgactga aagtccatct ttgagatatt gtaaattaag attgtggctg
      841 gcttattccg atcataacag aattgttggc cgtattgcag gaatttataa cccgagggct
      901 aacgaatacc ataataacaa gagaattagg tttggatggt ttgactttgt ggaggatatt
      961 gaggtcgctg ctgctcttct tggtaaagtt gaagagtggg gagtttcttt ggggatggaa
     1021 gagattcatg ggccgctcgg atataatact tggtatagac agggagtttt aattgaagga
     1081 tttgaaaaca caccgcctgc taactgtttg tataactatc cttattacag atctattct
//
LOCUS       DAOTQL010000279         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_107224_length_1134_cov_3.807229, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000279 DAOTQL010000000
VERSION     DAOTQL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107224_length_1134_cov_3.807229"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..551)
                     /locus_tag="P5293_09520"
                     /pseudo
     CDS             complement(1..551)
                     /locus_tag="P5293_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          263 a          296 c          272 g          303 t
ORIGIN      
        1 ccctcgattg tgagccgctt catttcagcc tttcggatct cagagaccac cttgtcatta
       61 aagttcttta ctatatgaaa gtagtcgaag accacctgga tgtgggggca tttttcctcg
      121 aatgcctcct ggaagtcgct gttcatgtcg caggcgactg catccacccc gttcatccac
      181 tccttgccca cgtgctcgat gaacgaatag accgaggcct tcttctttcc atgggcaagc
      241 cacaggatgt ggccggtctc catgtctatg atgaccactg catacttgtg cccgtcgtgg
      301 agcttgaact catcaatgcc caggaacctg gcctgcctct cgggctggat gagccgctct
      361 ccgtccacgg tgtacttctc tttcagccgc tctaggtcga tgtccttgac ggtgttcttt
      421 cccaagccgg tgatgtatgc aacttccttg ttggtaaagc cataggcgag caggtccctg
      481 gtatagttca acaactcgca gctgatgcgg tgtccccggg cctggaatgg gacggcctgc
      541 atgtggctat gaccgcatcg cggacaataa taccggagct tggagaacct cagacagctc
      601 agcttgtctc cgaaacacag atgcctcagg gtgacctcac agcggtcatg gatgtgcatc
      661 gagcttccgc acaatggaca caggcaatcc tcggcactgc ccttgagctc cccatggagc
      721 aggtacatct ctcgattgga tgcagtcgtt acgatttccg tcttgtaatt gtggaagcct
      781 accaatgatg aaggcaatgt gatagagtgc atatggggct gtcttcctgt ttgtgagttt
      841 ggttactaaa caatacaggg acggcccttc cttatgcaat catcttcaaa tcctctgaag
      901 ttctcgattc tctatacttt gacagctttg gccaatccca caggaaaagt gattgaccca
      961 aatttagcat atacttggag ttaaaaggag aggaacaatg cttttacaat tcaatttcaa
     1021 gaacttcaag tcctttcgcg atgatgcaac gttggatatg acggcaacaa aaatcactga
     1081 gttaagcggt cacgtaatac ctataggtaa tgaaaaagtg cttcctgttg cttc
//
LOCUS       DAOTQL010000280         1101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_112497_length_1101_cov_3.973231, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000280 DAOTQL010000000
VERSION     DAOTQL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112497_length_1101_cov_3.973231"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="P5293_09525"
     CDS             <1..555
                     /locus_tag="P5293_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRR50173.1"
                     /translation="FSAFIVMFAVIDILGSVPIIVGMKENNKKFNPFNASWISLVIMV
                     AFLFLGQALLGLFGVDISSFAVAGGLVLLVLAVEMIFGVQIFKDDGPTQSATIVPIVF
                     PLIAGAASFTTILSLRAEYEVINIIIALFINILIVYFVLSNIDYISQKIGKSGIYVMR
                     KFFGIILMAIAVKLIVSNLNSLIS"
     gene            584..>1101
                     /locus_tag="P5293_09530"
     CDS             584..>1101
                     /locus_tag="P5293_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRR50174.1"
                     /translation="MKYDFDEIIDRKGSDALKVEALKARWGREDLIPMWVADMDFRTP
                     PYIIDAIRKRCDAGILGYTSVPENWSESIINWLKTRYNWDISKDILTFSPGIVQGLAF
                     AIKCFTKQKDKIMVMSPVYHPFFLVSEHNNREVVYSSLKIENGSIEIDFEKFRTDIKG
                     CNVLILCNPHNPG"
BASE COUNT          335 a          195 c          207 g          364 t
ORIGIN      
        1 ttcagtgcat ttattgtaat gttcgctgta attgacattc tcggttcggt tccgatcatt
       61 gtcggaatga aagaaaacaa taaaaagttc aaccctttca atgcatcatg gatttcactg
      121 gtaatcatgg tggctttttt atttttagga caggcactcc tcggactttt tggagtagat
      181 atttcatcct tcgcagttgc aggaggactt gttctgctcg tccttgccgt tgaaatgatt
      241 ttcggcgttc aaatatttaa agatgatggt ccaactcagt cagccacaat agttcccata
      301 gttttccctc tcattgcagg tgctgcttcc tttactacta tactttctct cagagcagag
      361 tatgaagtaa taaatattat tatcgctctg tttataaata tactgattgt ctattttgta
      421 ctcagcaaca ttgattatat ttctcaaaaa ataggcaaaa gtggtattta tgtcatgcgt
      481 aaattttttg gcattatcct aatggccata gcagtaaaac ttatcgtctc taacctgaat
      541 tctctgataa gctgatcatt aaataacttg ctttttacag acaatgaaat atgattttga
      601 cgaaataata gacagaaaag gcagtgatgc tcttaaagtt gaagcactca aagcaagatg
      661 gggaagagaa gacttaatcc ccatgtgggt tgcagatatg gatttcagga ctccgccata
      721 tatcatcgac gctatcagaa aacgttgcga tgcaggcatt cttggttata caagtgtgcc
      781 tgaaaattgg tcagaatcaa taattaattg gcttaaaaca agatacaatt gggacatttc
      841 taaagatata ctgacttttt ctcccgggat tgttcaggga ttggcttttg caataaaatg
      901 ttttacaaaa cagaaagaca agataatggt aatgtcccct gtatatcacc ctttttttct
      961 tgtcagtgag cacaataaca gagaggtagt ttactcctca cttaaaattg agaatggcag
     1021 tattgagatt gatttcgaaa aattcagaac tgacattaaa ggatgtaatg tgcttattct
     1081 atgcaatcct cataatcccg g
//
LOCUS       DAOTQL010000281         1026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_126183_length_1026_cov_5.063852, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000281 DAOTQL010000000
VERSION     DAOTQL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126183_length_1026_cov_5.063852"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1026
                     /locus_tag="P5293_09535"
     CDS             <1..>1026
                     /locus_tag="P5293_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRR50175.1"
                     /translation="FIYKTALEHHLGEVAFFRVMSGKLTEGMDLTNPENGAKERISII
                     YAVAGKKKERVPEMMAGDIGCTVKLKSAKTNQTLNGGGKDWIFEEIEFPPSKFRTAIK
                     AKSEKDDEKLGEILNRTHAEDPTIVVEYSKELKQTILSGQGEHHINILKWHLTNTYKI
                     EVDLFPPKIPYRETITKVAAGDYRHKKQSGGAGQFGEVHLIIEPIVEGVPAGNKFKVE
                     GRDLILNIKGKEEYPLEWGGMLEYYNCIVGGAIDARFMPAILKGIMEKMEEGPLTGSY
                     ARDIRVFVYDGKMHPVDSNEISFKLAGRNAFKEAFKKAGPKIMEPIYNVEVMVPSDCM
                     GDVMSDL"
BASE COUNT          342 a          162 c          247 g          275 t
ORIGIN      
        1 tttttattta caaaactgcc cttgaacatc atcttggaga ggttgcattt tttagagtca
       61 tgtccgggaa actgactgaa gggatggatt tgacaaatcc ggaaaatggt gcaaaggaac
      121 gtatttccat tatttatgca gtcgcaggaa agaaaaaaga gagagtacct gaaatgatgg
      181 caggagatat aggttgtact gttaaactta agtctgccaa gacaaatcag acactcaatg
      241 gaggtggcaa ggattggatc tttgaagaga ttgaatttcc gccttcaaaa tttaggacag
      301 caatcaaggc aaagtctgag aaagatgatg aaaaactcgg tgagattctc aacagaactc
      361 atgcagagga tcctactatc gtagtcgaat actcaaaaga gctaaagcaa acaatcctga
      421 gcggacaggg agagcaccat ataaatattt taaaatggca tttgacaaat acttataaga
      481 ttgaggttga cttgtttcct cctaagatac cttataggga aactattaca aaagttgctg
      541 ctggcgatta cagacataag aaacagtccg gtggggctgg ccaattcgga gaagtgcatt
      601 taattattga accaatagta gaaggtgtgc ccgcaggaaa taaatttaaa gttgagggac
      661 gagaccttat tttgaatatc aaaggtaaag aagagtatcc acttgaatgg ggagggatgc
      721 tggaatatta taactgtatt gtaggtggcg ctattgatgc tcgttttatg ccggccattt
      781 taaaaggaat tatggaaaag atggaagagg gtccactcac tggatcatat gcccgtgata
      841 tcagagtttt tgtctatgac ggtaaaatgc atcctgtgga ttccaatgaa atttccttta
      901 aactggctgg acgtaacgca tttaaggagg ccttcaagaa agcaggtccc aaaataatgg
      961 aaccaatata caatgttgaa gttatggtgc caagtgactg tatgggtgat gttatgagtg
     1021 accttc
//
LOCUS       DAOTQL010000282         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_126793_length_1023_cov_3.743802, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000282 DAOTQL010000000
VERSION     DAOTQL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126793_length_1023_cov_3.743802"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="P5293_09540"
     CDS             complement(<1..394)
                     /locus_tag="P5293_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRR50176.1"
                     /translation="MVNIIPAIDIISGECVRLSKGKFSTKKVYSSSPVEVAKVYSDLG
                     CRRLHIVDLDGARSNHPENLKILEQIASVTQMDIQFGGGIKDENSLISALNAGASRVI
                     CGSVAIYDPELFVEWLNKYGSQRVILGAD"
     gene            complement(399..989)
                     /gene="hisH"
                     /locus_tag="P5293_09545"
     CDS             complement(399..989)
                     /gene="hisH"
                     /locus_tag="P5293_09545"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRR50177.1"
                     /translation="MMTIIIDYNTGNLFSLTAALNRLGSDYTISSAPEEILSADAVIL
                     PGVGEASNAFSSLKKKGLEKVIPQIKAPVLGICIGMQLMCAYSEEGDTKCLGIFPNIV
                     KKLCGQGIKVPHMGWNTVQMSDSPLFEGMANDTYFYFLHSFAPDCGQYTVARTVHGLQ
                     FSSALSKNNFYGTQFHPEKSGIVGMKLLENFLKIKI"
BASE COUNT          312 a          214 c          169 g          328 t
ORIGIN      
        1 aatctgctcc aagaatcact ctttgagaac catatttatt aagccattcg acaaacaact
       61 ctggatcata aattgcaaca cttccgcaaa ttactcttga agcaccggca ttcaaggctg
      121 aaatcaaaga attttcatct tttatgccac caccaaattg aatgtccatt tgtgtcacag
      181 aggctatctg ttcaagtatt ttgagatttt caggatgatt acttcttgct ccgtcgagat
      241 ctacaatatg caaccttcta cagcccaaat cactgtaaac ttttgctacc tccaccggcg
      301 aagatgaata tacttttttt gtagaaaact ttcccttaga gagcctgacg cattctccag
      361 aaataatatc tatagctgga attatattaa ccattttatc aaattttaat ttttaaaaaa
      421 ttttccagaa gtttcattcc aactattcca cttttctctg gatggaactg cgtcccataa
      481 aaattatttt ttgaaagcgc cgaactgaat tgtaagccat gtactgttcg agctacagtg
      541 tattggccac agtcaggggc aaatgagtgt aaaaaataga aatatgtatc attggccata
      601 ccctcaaaaa gtggagaatc tgacatttga acagtattcc atcccatatg aggcacttta
      661 atgccttgcc cgcacagttt ttttacaata ttgggaaata tgccaagaca tttcgtatct
      721 ccttcttcac tgtaggcaca catcagttgc ataccaatac atatcccgag aaccggagcc
      781 ttaatttggg ggataacttt ctctaaacct ttttttttca aagatgagaa agcgtttgaa
      841 gcttctccta caccgggaag aattactgca tctgctgata aaatctcttc cggggctgat
      901 gagattgtat aatccgagcc caatctgttc aatgcggctg taagtgaaaa aagattcccc
      961 gtattgtagt caataattat tgtcatgatt tattataata gaccttttgt agttggtatt
     1021 tca
//
LOCUS       DAOTQL010000283         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2818
            NODE_127821_length_1018_cov_3.280374, whole genome shotgun
            sequence.
ACCESSION   DAOTQL010000283 DAOTQL010000000
VERSION     DAOTQL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867842
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 16:54:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,846
            Genes (coding)                    :: 1,831
            CDSs (with protein)               :: 1,831
            Genes (RNA)                       :: 65
            tRNAs                             :: 62
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 3 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127821_length_1018_cov_3.280374"
                     /isolate="AMR_MDS_2818"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..872)
                     /locus_tag="P5293_09550"
     CDS             complement(213..872)
                     /locus_tag="P5293_09550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRR50178.1"
                     /translation="MKFKGAIFDLDGTLVDSIEDLAESMNTVLKENNFPEHEIDKYKL
                     FVGNGIKTLVRRSLPAGSDEETVSKCYLSMMNVYDNNCVSKSRPYDGIRELLQEFTDK
                     KIKLAVLSNKADNLTKKIIYSLFPEVPFVKVVGLVDENLKKPNPESALAICREMHASP
                     DEVLFLGDTAVDMQTAKKGGFVAIGVLWGFRGKDELIEAGADIIVEKPVDLMNFLDSK
                     R"
     gene            complement(884..>1018)
                     /locus_tag="P5293_09555"
     CDS             complement(884..>1018)
                     /locus_tag="P5293_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HRR50179.1"
                     /translation="MGGGIGQSRLCMLLLQKCHIGEVQSSMWPEETLRTCAERGVFIF
                     "
BASE COUNT          313 a          242 c          142 g          321 t
ORIGIN      
        1 agcgtatact gccatcaaat gtatattctc ccctcttcca actcgcacaa agccctccgg
       61 gaacagcatt tttttcgaaa atctcagttt caaaaccata catctgaagg taacaaccta
      121 atgtcaggcc tgacattcct ccacctatta tgttaacctt catgtaatat catttgagac
      181 tcttcaaatt tacaataaaa ttaaattgtt agctatcttt tgctatccaa aaaattcatt
      241 aaatctactg gtttttctac tataatatct gcgccagcct caattaactc atctttgcct
      301 ctaaagcccc acaaaacgcc gattgcaaca aagccgccct ttttggcagt ttgcatatct
      361 acagccgtat cgcctaaaaa aagaacttcg tctggggaag cgtgcatttc tctacaaatt
      421 gcaagggcac tttcaggatt cggcttctta agattttcat ccacaagacc aactaccttt
      481 acaaatggaa cctcagggaa aagcgagtaa ataatctttt ttgtaagatt atccgctttg
      541 ttcgaaagca cggcaagttt tatctttttg tcggtaaatt cttgcaaaag ctccctgatt
      601 ccatcatatg gcctgctttt gctgacacag ttgttatcat aaacattcat cattgacaga
      661 taacactttg agacagtctc ctcatcacta ccagcaggaa gagatcttct gacaagtgtt
      721 tttataccat ttccaacgaa caatttatac ttgtcaattt catgttctgg aaaattattt
      781 tcttttaata cagtattcat tgactctgcc agatcttcaa ttgaatccac aagagtgccg
      841 tcaaggtcaa aaatagctcc tttaaatttc ataaaaacat tctttaaaaa ataaaaactc
      901 ctctttcagc acaagttcgc agagtctctt caggccacat ggaagattga acctcaccaa
      961 tatgacattt ttgcaacaat agcatgcaaa gccttgattg acctattcct cctcccat
//
